TY - JOUR. T1 - Human Amniotic Fluid Stem Cells. T2 - Therapeutic Potential for Perinatal Patients with Intractable Neurological Disease. AU - Ochiai, Daigo. AU - Masuda, Hirotaka. AU - Abe, Yushi. AU - Otani, Toshimitsu. AU - Fukutake, Marie. AU - Matsumoto, Tadashi. AU - Miyakoshi, Kei. AU - Tanaka, Mamoru. PY - 2018/12/26. Y1 - 2018/12/26. N2 - Mesenchymal stem cells (MSCs) have generated great interest in the fields of regenerative medicine and immunotherapy because of their unique biological properties. Among MSCs, amniotic fluid stem cells (AFS) have a number of characteristics that make them attractive candidates for tissue engineering and cell replacement strategies, particularly for perinatal medicine. If various neonatal conditions, including birth asphyxia, preterm birth, and congenital abnormalities, which result in long-lasting severe impairments, could be predicted during pregnancy, it would allow collection of small samples of amniotic fluid cells by amniocentesis. In vitro ...
Firstly, we analyzed hAFC samples for the expression of germ cell markers; in agreement to previous reports [11], we observed a consistently high expression of OCT4 and CD133. However, when we examined additional markers we did not observe expression of genes specific to germ cell differentiation. In this regard, it was reported that hAFCs had the ability to form embryoid bodies (EBs) when cultured under conditions without anti-differentiation factors and without contact to feeder cells. Moreover, the formation of such three-dimensional multicellular aggregates was accompanied by a decrease in stem cell marker expression and by the induction of differentiation into different cell lineages [23]. Hence herein hAFCs kept under a stem cell culture system yielded a high number of clones that when cultured in suspension without anti-differentiation factors, spontaneously formed multicellular EBs and harbored high differentiation potential. Moreover, when we cultured EBs in the presence of germ cell ...
TY - JOUR. T1 - Ethanol alters the osteogenic differentiation of amniotic fluid-derived stem cells. AU - Hipp, Jennifer A.. AU - Hipp, Jason D.. AU - Atala, Anthony. AU - Soker, Shay. PY - 2010/10/1. Y1 - 2010/10/1. N2 - Background: Fetal alcohol spectrum disorder (FASD) is a set of developmental defects caused by prenatal alcohol exposure. Clinical manifestations of FASD are highly variable and include mental retardation and developmental defects of the heart, kidney, muscle, skeleton, and craniofacial structures. Specific effects of ethanol on fetal cells include induction of apoptosis as well as inhibition of proliferation, differentiation, and migration. This complex set of responses suggests that a bioinformatics approach could clarify some of the pathways involved in these responses. Methods: In this study, the responses of fetal stem cells derived from the amniotic fluid (AFSCs) to treatment with ethanol have been examined. Large-scale transcriptome analysis of ethanol-treated AFSCs ...
In recent years, great interest has been devoted to the use of Induced Pluripotent Stem cells (iPS) for modeling of human genetic diseases, due to the possibility of reprogramming somatic cells of affected patients into pluripotent cells, enabling differentiation into several cell types, and allowing investigations into the molecular mechanisms of the disease. However, the protocol of iPS generation still suffers from technical limitations, showing low efficiency, being expensive and time consuming. Amniotic Fluid Stem cells (AFS) represent a potential alternative novel source of stem cells for modeling of human genetic diseases. In fact, by means of prenatal diagnosis, a number of fetuses affected by chromosomal or Mendelian diseases can be identified, and the amniotic fluid collected for genetic testing can be used, after diagnosis, for the isolation, culture and differentiation of AFS cells. This can provide a useful stem cell model for the investigation of the molecular basis of the diagnosed
Bone tissue engineering strategies require cells with high proliferative and osteogenic potential as well as a suitable scaffold to support the development of these as they form new bone tissue. In this study, we evaluated whether the differentiation stage of amniotic fluid stem cells (AFSC) could enhance the regeneration of critical sized femoral defects in a rat model. For this purpose, AFSC were seeded onto a starch-poly(ε-caprolactone) (SPCL) scaffold and were cultured in vitro in osteogenic culture media for different periods of time in order to obtain: i) undifferentiated cells, ii) cells committed to the osteogenic phenotype and iii) "osteoblast-like" cells. In vitro results indicate that AFSC were considered to be osteogenically committed by the end of week 2 and osteoblastic-like after week 3 in culture. Constructs composed of AFSC-SPCL scaffolds from each differentiation stage were implanted into critical sized femoral defects. The quality of new tissue formed in the defects was ...
Vascular Endothelial Growth Factor Up-Regulation in Human Amniotic Fluid Stem Cell Enhances Nephroprotection after Ischemia-Reperfusion Injury in the Rat The authors evaluated if the up-regulation of vascular endothelial growth factor strengthens the protective effect of amniotic fluid stem cells in a renal ischemia-reperfusion injury model. [Crit Care Med] Abstract Secretome of Human Fetal Mesenchymal Stem Cell Ameliorates Replicative Senescence Researchers hypothesized that the bioactive factors secreted by mesenchymal stem cells (MSC) isolated from human fetal tissues (hFMSC), collectively named as hFMSC secretome, could possess beneficial effects on human adult MSC undergoing replicative senescence, thus promoting their capability of proliferation and differentiation. [Stem Cells Dev] Abstract A Novel High Throughput Screening Platform Reveals an Optimized Cytokine Formulation for Human Hematopoietic Progenitor Cell Expansion Scientists used combinatorial cell culture technology ...
Scientists hailing from the Wake Forest Institute for Regenerative Medicine (WFIRM) have been putting their efforts into a revolutionary approach for helping chronic kidney disease sufferers get a firmer grasp on their situation and repair any tissues that may have been ravaged by the disease. This solution comes in the form of human amniotic fluid-derived stem cells, which the scientists have posited would not only repair tissue, but even help recover kidney function that has been lost. The researchers at WFIRM have been at the cutting edge of stem cell research for quite some time, with their teams also pioneering the equally impressive 3D bioprinting of organs and tissues. According to the scientists, the amniotic fluid-derived stem cells are unique in that they can be utilized as universal cell sources, meaning they can fill in for numerous different types of cells, while simultaneously benefiting the entirety of the organism with anti-inflammatory properties and potential ...
Epithelial and fibroblast cells were obtained from cultures of amniotic fluid cells. Epithelial cells demonstrated high activities of histidase. In contrast, histidase activity was not detected in fibroblasts derived from the same original culture. This observation indicates that cultures of amniotic fluid cells consist of cells with different biochemical properties as well as morphological characteristics. ...
Whether these cells are as versatile as embryonic stem cells remains to be determined," said Atala, "but the current finding is certainly encouraging.". Atala stopped short of calling the cells pluripotent, which means the ability to form many cell types. He said while the cells meet some of the characteristics of pluripotency, such as versatility, they do not form tumors when implanted in animals, which is also considered a characteristic. The fact that the amnion cells are less likely to form tumors may be one advantage that they have over embryonic stem cells in their potential for clinical use.. Co-researchers were Alessandro Valli, Ph.D., Margit Rosner, student, Christiane Fuchs, MSc., Nicol Siegel, MSc., and Helmut Dolznig, Ph.D., from the Medical University of Vienna, Colin E. Bishop, Ph.D., from Wake Forest, and Ulrike Mädel, student, and Wilfried Feichtinger, M.D., from Wunschbaby Zentrum, in Vienna, Austria.. About the Wake Forest Institute for Regenerative ...
Amniotic fluid is concentrated in hyaluronic acid, stem cells, cytokines, and proteins. These important compounds are the fundamental cells existing in the body. Hyaluronic acid is a naturally occurring liquid found in the knee that helps to cushion the knee and can help sooth any joint.. You may have heard about amniotic fluid injections and wondered if this treatment option was right for you and your situation. The best way to find out is to discuss your condition with one of our physicians so you can be evaluated. These are some common conditions that can benefit from amniotic fluid injections:. ...
Abstract: Deficiency of glycosidases is a fundamental feature of the hereditary diseases of glycoconjugate accumulation. The data obtained on activity of glycosidases in cell culture of normal amnionic fluid might be used as standards in prenatal diagnostics of hereditary glycolipidoses and glycoproteinoses. Use of cell culture of amnionic fluid for prenatal diagnosis of Tay-Sachs disease is ...
Restricted Item. Print thesis available in the University of Auckland Library or available through Inter-Library Loan. Three major cell types, termed E, F and AF, constitute cultures of second trimester human amniotic fluids and are widely used for the prenatal diagnosis of inherited disorders of metabolism. Current evidence suggests that the predominant cell type, the AF cells, retain properties of trophoblast and it has been proposed that they are derived from the trophoblast of the placenta. Since the trophoblast is thought to be the site of the aromatization of C19 -steroids in the placenta, it was considered that AF type amniotic fluid cell cultures should possess this steroidogenic activity. Investigations were therefore undertaken to explore this hypothesis. Initial experiments involved culturing AF and F type cells in the presence and absence of dehydroepiandrosterone sulphate and subsequently incubating the cell layers with [4-14C] androstenedione. Isolation and identification of the ...
Tendons are highly prone to injury and the intrinsic hypocellularity and hypovascularity make their natural healing extremely slow and inefficient when severed damaged. Surgical repair with grafts is common but unsuccessful in a long term basis. The development of tissue engineering strategies based on stem cells explores a natural endogenous system of regeneration with potential for tendon application. We propose to establish biochemical culturing conditions to assess the tenogenic potential of human adipose stem cells (hASCs) and amniotic fluid-derived stem cells (hAFSCs), known for their proliferative and differentiation capacities. Since several growth factors (GFs) participate in tendon formation and ECM synthesis, these GFs were added to the culture medium to stimulate tenogenic differentiation of these cells. This study also envisions the application of hASCs and/or hAFSCs in cell-based strategies for tendon repair ...
The usage of stem cells is a promising strategy for the repair of damaged tissue in the injured brain. Recently, amniotic fluid (AF) cells have received a lot of attention as an alternative source of stem cells for cell-based therapies. However, the
In a previous study, we isolated human amniotic fluid (AF)-derived mesenchymal stem cells (AF-MSCs) and utilized normoxic conditioned medium (AF-MSC-norCM) which has been shown to accelerate cutaneous wound healing. Because hypoxia enhances the wound healing function of mesenchymal stem cell-conditioned medium (MSC-CM), it is interesting to explore the mechanism responsible for the enhancement of wound healing function. In this work, hypoxia not only increased the proliferation of AF-MSCs but also maintained their constitutive characteristics (surface marker expression and differentiation potentials). Notably, more paracrine factors, VEGF and TGF-β1, were secreted into hypoxic conditioned medium from AF-MSCs (AF-MSC-hypoCM) compared to AF-MSC-norCM. Moreover, AF-MSC-hypoCM enhanced the proliferation and migration of human dermal fibroblasts in vitro, and wound closure in a skin injury model, as compared to AF-MSC-norCM. However, the enhancement of migration of fibroblasts accelerated by AF-MSC-hypoCM
I am 5 weeks into my first pregnancy and I dont know if Im being paranoid or properly cautious. I dont even know if I can leak amniotic fluid at this stage and as my first appointment with my OB isn...
Abbildung 1: Detection of Oct-4 in the nucleus of a human amniotic fluid cell. A phase contrast picture of an amniotic fluid cell population grown under culture conditions used for routine prenatal diagnosis of genetic mutations is presented. For immunocytochemical analysis of cellular Oct-4 expression, cells were fixed and incubated with anti-Oct-4 antibody. Thereafter, cells were washed, incubated with a biotinylated secondary antibody, washed again, and incubated with ExtrAvidin-Cy3 conjugate. One amniotic fluid cell with the red Oct-4-specific signal in the nucleus is shown ...
The CD117 MicroBead Kit has been developed for the positive selection of human hematopoietic stem and progenitor cells from peripheral blood, cord blood or bone marrow. It has also been used for the isolation of various stem cells from adult tissues, e.g. amniotic fluid stem cells1 and cardiomyocyte progenitors2 . - 中国
The CD117 MicroBead Kit has been developed for the positive selection of human hematopoietic stem and progenitor cells from peripheral blood, cord blood or bone marrow. It has also been used for the isolation of various stem cells from adult tissues, e.g. amniotic fluid stem cells1 and cardiomyocyte progenitors2 . - Belgique
1. Steele MV, Breg VR jr. Chromosome analysis of human amniotic fluid cells. Lancet 1966; 1: 383- 385. 2. Valenti C, Schutta EF, Kehaty T. Cytogenetic diagnosis of Downs syndrome in utero. Am J Med Assoc 1969; 207: 1513. 3. Ondrejčák M. Prenatálna cytogenetická diagnostika z buniek plodovej vody. Lek Obzor 1985; 34(6): 329- 333. 4. von Eggeling F, Freytag M, Fahsold R et al. Rapid detection of trisomy 21 by quantitative PCR. Hum Genet 1993; 91(6): 567- 570. 5. Divane A, Carter NP, Spathas DH et al. Rapid prenatal diagnosis of aneuploidy from unculltured amniotic fluid cells using five - colour fluorescence in situ hybridization. Prenat Diagn 1994; 14(11): 1061- 1069. 6. Pertl B, Yau SC, Sherlock J et al. Rapid molecular method for prenatal detection of Downs syndrome. Lancet 1994; 343: 1197- 1198. 7. Kuo WL, Temjin H, Segraves R et al. Detection of aneuploidy involving chromosomes 13, 18 and 21 by fluorescence in situ hybridization (FISH) to interphase and metaphase amniocytes. Am J Hum ...
Background: Spontaneous ascitic fluid infection (SAI) is an acute infection of ascitic fluid. SAI has three subtypes; SBP, CNNA, MNB. The prototype is Spontaneous bacterial peritonitis (SBP). SBP is a frequent and serious complication in patients with cirrhosis leading to significant mortality and morbidity. Diagnosis of SBP is mainly established by elevated polymorphonuclear leukocyte count and positive ascitic fluid bacterial culture. Methodology: Records of patients with suspected diagnosis of Spontaneous ascitic fluid infection were analyzed in the present study. Patients admitted in the last one year i.e., between January 2014 to December 2014 with ascites secondary to cirrhosis were included. All patients with decompensated cirrhosis had undergone ascitic fluid paracenetesis after admission to the hospital. Ascitic fluid was centrifuged and analyzed for Total protein, Albumin, Total and Differential leukocyte count. Ascitic fluid was cultured by using BacTec method to determine the growth ...
This study showed that term amniotic fluid is a relatively rich source of MSCs having high expansion capacities and features similar to MSCs isolated from other sources. We also showed that harvesting amniotic fluid at term caesarean section deliveries by catheter resulted in much higher collection volumes compared with 10-20 ml volumes that were harvested in previous studies of term or pre-term amniotic fluid [24-26]. We were able to harvest on average about 400 ml of fluid from the 20 samples. Comparing the TAF-MSCs described in this study with MSCs isolated from BM and AD, we show that they share a significantly similar transcriptional profile. Taken together, these results suggest that TAF-MSCs may be an alternative and complimentary source of MSCs with features making them highly attractive for further investigation in in-vivo pre-clinical functional assessments.. There are only a limited number of publications characterizing term human amniotic fluid cells [24-26]. In line with these ...
Question - Diagnosed with severely low amniotic fluid levels. On medication. Will amniotic fluid replenish? . Ask a Doctor about Amniotic fluid, Ask a General & Family Physician
The miRNAs are a new class of nucleic acids that show promise as useful clinical biomarkers for several diseases (6-8). Our data revealed that the expressions of miR-125b-2, miR-155, and miR-3156 in the amniotic fluid culture were significantly higher in pregnant women carrying fetuses with DS than in the control group. The study sample size was small; however, to the best of our knowledge, our study is the first to demonstrate significant differences in miRNA expression in cell cultures between fetuses with and without DS. Many studies have evaluated the association of miRNA expression levels with a variety of pathologic conditions, including preeclampsia, fetal congenital cardiac defects, neural tube defects, fetal growth retardation, and DS (9-15). The miRNAs encoded by hsa 21 (i.e., hsa-miR-99a, let-7c, miR-125b-2, miR-155, and miR-802) have been proven to correlate with the complex and variable phenotypes of DS and were shown to be overexpressed in the heart, frontal cortex, and hippocampus ...
Chang Medium® D was developed for the primary culture of human amniotic fluid cells for use in karyotyping and other antenatal genetic testing. This formula has been optimized for both flask and in situ methodologies. Packaged in single bottles, ready-to-use with no mixing required. For in vitro diagnostic use.. ...
De Coppis team has demonstrated that intravenous transplantation of amniotic fluid stem (AFS) cells enhances the muscle strength and improves the survival rate of the affected animals. This is the first study to demonstrate the functional and stable integration of AFS cells into skeletal muscle, highlighting their value as a cell source for the treatment of muscular dystrophies.. However, the research is still at a relatively early stage as the work has only been carried out in animal models.. Dr Coppi said: "Spinal muscular atrophy is a genetic disease affecting one in 6,000 births. It is currently incurable and in its most severe form children with the condition may not survive long into childhood. Children with a less severe form face the prospect of progressive muscle wasting, loss of mobility and motor function. There is an urgent need for improved treatments.. "We are excited by this potential new approach for regenerating skeletal muscle tissue, but much more research is needed. We now ...
The next, I would be plagued with worry that despite all my efforts at natural induction-sex, acupuncture, membrane stripping-that I would reach 42 weeks and, according to my home birth midwives policy, no longer be able to have the home birth that I wanted. And then I would envision a whole host of unlikely and horrendous birth defects that might be the reason I wasnt going into labor, and also the complete financial disaster that would ensue once we had our complicated hospital birth of our very sickly child on our not-so-great insurance policy.. And then, it happened. I had to jump headfirst into my hospital birth fear and let go of the last shred of control I thought I might have when my midwife said, "This looks like a baby that wants to be born in the hospital.". At a routine non-stress test (used to assess the health of the baby by monitoring its heart rate and amniotic fluid levels) the nurse saw one significant deceleration of our babys heart rate. And my amniotic fluid levels were ...
The next, I would be plagued with worry that despite all my efforts at natural induction-sex, acupuncture, membrane stripping-that I would reach 42 weeks and, according to my home birth midwives policy, no longer be able to have the home birth that I wanted. And then I would envision a whole host of unlikely and horrendous birth defects that might be the reason I wasnt going into labor, and also the complete financial disaster that would ensue once we had our complicated hospital birth of our very sickly child on our not-so-great insurance policy.. And then, it happened. I had to jump headfirst into my hospital birth fear and let go of the last shred of control I thought I might have when my midwife said, "This looks like a baby that wants to be born in the hospital.". At a routine non-stress test (used to assess the health of the baby by monitoring its heart rate and amniotic fluid levels) the nurse saw one significant deceleration of our babys heart rate. And my amniotic fluid levels were ...
After a long wait we had the follow-up ultrasound today. Owen has been a very active boy for a while now which has helped us make it through the wait with much optimism. The ultrasound was at 1:30 and our whole clan was allowed into the ultrasound room. The clan being my mom and dad, Joshs mom and sister and of course Josh and I. As the ultrasound progressed every update was better than the last. First off the amniotic fluid level was right on (high amniotic fluid levels are common with trisomy 18 babies). His choroid plexus cysts had resolved, as well as the calcium deposit on his heart. They were just gone, they said there was a good chance they would resolve, but to see it live in person was nothing short of a miracle for us!!! And finally all of the measurements were done and he came in at 3 lbs. 1oz. and measured within one day of his due date, which we were informed never happens. The measurement was the most important thing to us. The geneticist in Little Rock had told us that babies ...
Looking for Non-parametric statistics? Find out information about Non-parametric statistics. Any method of inference that does not depend on the characteristics of the population from which the samples are obtained Explanation of Non-parametric statistics
Health, ...In a breakthrough that may help fill a critical need in stem cell rese...The Mount Sinai researchers found that when compared to cultured adult... There remains today a need in stem cell research for an easily reprog...Amniotic fluid skin cells can be safely obtained from pregnant women u...,Amniotic,fluid,cells,more,efficiently,reprogrammed,to,pluripotency,than,adult,cells,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
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Intra-amniotic infections are associated with an increased risk of preterm delivery, which, in turn, may be associated with neurological sequelae in former preterm infants [80]. Microbial presence in the amniotic fluid may elicit maternal and fetal inflammatory response that are then responsible for neonatal complications. The association between elevated inflammatory cytokines IL-1β and IL-6 in the amniotic fluid and subsequent white matter injury has been noted in preterm infants [81]. Elevated levels of inflammatory cytokines in the cord blood including IL-1β, IL-6 IL-8, and TNF-α have also been shown to correlate with neonatal cerebral lesions as detected by MRI after parturition in human premature infants [82]. Furthermore, clinical evidence shows that elevated inflammatory response in the perinatal period has been demonstrated to correlate with long-term neonatal morbidities including cerebral palsy [83], psychomotor deficits [8], and non-neurological diseases including necrotizing ...
Definition : Immunoassay reagents intended to perform quantitative analyses on amniotic fluid samples obtained from pregnant women to determine the ratio of phospholipids (i.e., surfactants) to albumin. These tests are typically used to estimate fetal lung maturity and to assess the associated risk of respiratory distress syndrome if birth occurs. The predictive values are less than 40mg/g for immature lungs and more than 70mg/g for mature lungs, but values may change as pregnancy progresses.. Entry Terms : "Reagents, Immunoassay, Pregnancy, Phospholipid/Albumin Ratio". UMDC code : 20669 ...
Objective: RANTES (regulated on activation, normal T cell expressed and secreted), a potent and versatile chemokine, is capable of attracting monocytes, lymphocytes, basophils, and eosinophils. This cytokine has been implicated in the regulation of the inflammatory response and in the recruitment of macrophages to the implantation site in early pregnancy. RANTES messenger ribonucleic acid and protein have been detected in fetal tissue and first-trimester trophoblast in response to bacterial endotoxin. The purpose of this study was to determine whether intrauterine infection, parturition (preterm and term), and gestational age affect the amniotic fluid concentrations of RANTES in human pregnancy. Study Design: A cross-sectional study was designed to examine the relationship between labor, microbial invasion of the amniotic cavity, gestational age, and RANTES expression in amniotic fluid. Amniotic fluid was obtained from 214 women in the following groups: (1) midtrimester (n = 22), (2) preterm ...
Our findings confirm the hypothesis that medical induction of labour is related to an increased risk of AFE," added Dr. Kramer. "Although the absolute risk increase of AFE for women is very small (four or five total cases and one or two fatal cases per 100,000 women induced) and is unlikely to affect the decision to induce labour in the presence of compelling clinical indications, women and physicians should be aware of this risk if the decision is elective." ...
Our findings confirm the hypothesis that medical induction of labour is related to an increased risk of AFE," added Dr. Kramer. "Although the absolute risk increase of AFE for women is very small (four or five total cases and one or two fatal cases per 100,000 women induced) and is unlikely to affect the decision to induce labour in the presence of compelling clinical indications, women and physicians should be aware of this risk if the decision is elective ...
Scientist have identified a link between exposure to high levels of oestrogen sex hormones in the womb and the likelihood of developing autism. The discovery adds further evidence to support the prenatal sex steroid theory of autism first proposed 20 years ago.. In 2015, a team of scientists at the University of Cambridge and the State Serum Institute in Denmark measured the levels of four prenatal steroid hormones, including two known as androgens, in the amniotic fluid in the womb and discovered that they were higher in male foetuses who later developed autism. These androgens are produced in higher quantities in male than in female foetuses on average, so might also explain why autism occurs more often in boys. They are also known to masculinise parts of the brain, and to have effects on the number of connections between brain cells.. Today, the same scientists have built on their previous findings by testing the amniotic fluid samples from the same 98 individuals sampled from the Danish ...
The present study investigated: (a) the presence of antiphospholipid antibodies and (b) the obstetric outcome in healthy pregnant women showing false-positive TORCH-Toxoplasmosis, Other: syphilis, varicella-zoster, Rubella, Cytomegalovirus (CMV), and Herpes infections-results. Data from 23 singleton healthy pregnancies with false-positive TORCH results were collected. Each woman was systematically screened for TORCH IgG and IgM during the pre-conception assessment and/or at the beginning of pregnancy. In the presence of IgM positivity, when indicated (CMV, toxoplasmosis, rubella, herpes simplex virus), IgG avidity was evaluated and, if possible, polymerase chain reaction was performed on an amniotic fluid sample in order to distinguish between primary infection or false positivity ...
Not only does death linger on as a memory in the nervous system, but suicides will often choose a method that reflects the prototype of their birth experiences. So, those strangled on the umbilical cord may hang themselves, or an overdose of pills is chosen by someone who was drugged at birth. Why? Because of the prototype; for a neonate strangling on the cord, further strangling would have ended the agony. Those drowning in amniotic fluid at birth will opt for death by drowning. Case in point: the writer-actor Spalding Gray had a lifetime obsession with drowning. He used to swim in the ocean as far out as he possibly could until the point of exhaustion and then struggled to try and make it back. He killed himself by jumping off the Staten Island Ferry in the middle of the night. I hypothesize that he may have been drowning at birth and the end of the original sequence would be drowning. In other words, I think he died like he was born. This is never to minimize the pain in the present; ...
Not only does death linger on as a memory in the nervous system, but suicides will often choose a method that reflects the prototype of their birth experiences. So, those strangled on the umbilical cord may hang themselves, or an overdose of pills is chosen by someone who was drugged at birth. Why? Because of the prototype; for a neonate strangling on the cord, further strangling would have ended the agony. Those drowning in amniotic fluid at birth will opt for death by drowning. Case in point: the writer-actor Spalding Gray had a lifetime obsession with drowning. He used to swim in the ocean as far out as he possibly could until the point of exhaustion and then struggled to try and make it back. He killed himself by jumping off the Staten Island Ferry in the middle of the night. I hypothesize that he may have been drowning at birth and the end of the original sequence would be drowning. In other words, I think he died like he was born. This is never to minimize the pain in the present; ...
We just had our 20 week anatomy scan today, and for the most part things looked good with the exception of some renal abnormalities. DH was born with whats called vesiculoureteral reflux and had to have surgery as a baby to reattach his ureters to his bladder. Based on todays scan, we think that may be whats going on with our first LO. She has dilated collecting systems, normal kidneys,, normal bladder, and normal amniotic fluid levels. We are being referred to the Pediatric Nephrologist (who DH and I both work with and adore), as well as a Pediatric Urologist (not even a part of our hospital). Well be getting ultrasounds every 4 weeks now, with both the MFM and Nephrologists there at those appointments. I feel like we have a plan, and Im only slightly having alarm bells go off. I hope this is what DH had - as DH has normal kidney function as an adult. Does anyone have any experience with kidney issues in particular, or with getting a vague diagnosis like this and having to increase ...
An automated analytical instrument for conducting assays for components of interest in fluid samples. The instrument includes a testing system for assaying a fluid sample for a component of interest, a fluid dispensing assembly, an assembly for providing an assay module to the testing system and a fluid sample holding tray transport assembly for transporting a fluid sample holding tray carrying fluid sample holding containers to the fluid dispensing assembly and away from the fluid dispensing assembly. The transport assembly includes a conveyor, a fluid sample holding tray loading unit and a fluid sample holding tray unloading unit. The conveyor includes a carriage which is slidably mounted on a rail and moved along the rail by an endless belt which is fixedly attached to the carriage and driven by a reversible motor. In the operation of the instrument, one or more fluid sample holding trays are placed onto the fluid sample holding tray loading unit. Each fluid sample holding tray so loaded on to the
After a short nap while Joe and Grandpa took Brendan for a walk, we left Brendan in the care of his grandparents as we left for my ultrasound appointment. As we were well aware, the ultrasound was scheduled in order to determine if the baby was actually measuring the size she was supposed to be and to see if the amniotic fluid was low. So it wasnt a complete shock when the ultrasound tech stated that the baby was, in fact, average size for a 37 week fetus and not a 39 week fetus. And then proceeded to show us that there was very little fluid for measurement, leaving me with whats called a low amniotic fluid index. As a labor and delivery nurse I knew the implications of the ultrasounds results and while an AFI of 5 (normal of 10-25) is not extremely low, its low enough to warrant some concern. We were told to wait while the tech paged the doctor and I turned to Joe and explained that one of two things was likely to happen. I would either need to go to the maternity unit for monitoring (to ...
A proper canine neonatal assistance, required to reduce the high perinatal loss rate, imply a full knowledge about the fetal-to-neonatal physiology. Because fetal fluids play an important role throughout mammals pregnancy, influencing fetal growth and development, fetal well being, and contributing to guarantee the most suitable environment for the fetus, the knowledge about fetal fluids biochemical composition is of major importance. At first, the biochemical composition of fetal fluids collected by normal developed, healthy and viable newborns, is necessary to depict the normal features, and represent the first step for the further detection of abnormalities associated to fetal/neonatal distress and useful for the early identification of newborns needing special attention, immediately after birth ...
Ponevadz jsem se posledni dobou zajimal o NFS pod Linuxem (u portmapu v RedHatu se pise ye tam jsou odstraneny zname chyby NFS a NIS) narazil jsem na AFS. Vsude se totiz pise ze NFS neni moc bezbecne. Muze min nejakz odbornik rici jak je to s bezpecnosti u AFS? Myslim ze ve verzi 2.2 jiz bude defaultne ve zdrojacich jadra. Pokud se nemylim AFS ma jiz RedHat 5.1 Mohl by tedy nekdo zhodnotit vyhody a nevyhody AFS a NFS P.S.: Mimochodem nevite proc v RedHatu neni PCNFS daemon a jak je to s Debianem? P.S.S: Jaky je rozdil mezi PCNFS a BWNFS (zajima mne hlavne bezpecnost ...
Apply with brush to cover entire screen with screen filler; when the screen filler is dry, the drawing fluid can be washed out, allowing for the area to be printed.
A device for transporting a plurality of similarly sized beverage cans includes a transparent, flexible pouch of fixed dimensions, and a rigid housing in which the pouch is slidably received. The front and rear walls of the pouch and housing are spaced apart a distance about equal to the length of the cans, so that when the cans are inserted with their ends adjacent the front and rear walls, they will remain in unidirectional alignment upon removal of the pouch from the housing. The pouch includes a flap for keeping the cans in the pouch, and adhesive tape for securing the flap to the front of the pouch. The tape passes through a facial opening in the housing in order to seal the pouch while it is in the housing. The pouch is preferably transparent so that the number of cans can be accurately counted, and so that it can be visually confirmed that all of the cans are of the type returnable for a refund of any deposit. In one preferred embodiment the housing and pouch walls serve as the sole means for
Current treatments for tendon injuries often fail to fully restore joint biomechanics leading to the recurrence of symptoms, and thus resulting in a significant health problem with a relevant social impact worldwide. Cell-based approaches involving the use of stem cells might enable tailoring a successful tendon regeneration outcome. As growth factors (GFs) powerfully regulate the cell biological response, their exogenous addition can further stimulate stem cells into the tenogenic lineage, which might eventually depend on stem cells source. In the present study we investigate the tenogenic differentiation potential of human- amniotic fluid stem cells (hAFSCs) and adipose-derived stem cells (hASCs) with several GFs associated to tendon development and healing; namely, EGF, bFGF, PDGF-BB and TGF-β1. Stem cells response to biochemical stimuli was studied by screening of tendon-related genes (collagen type I, III, decorin, tenascin C and scleraxis) and proteins found in tendon extracellular matrix (ECM)
What is fetal lung maturity? Fetal lung maturity measures the amount of surfactant in amniotic fluid. Deficiency causes respiratory distress.