TY - JOUR. T1 - Nrf2 activation inhibits effects of thrombin in human amnion cells and thrombin-induced preterm birth in mice. AU - Chigusa, Yoshitsugu. AU - Kishore, Annavarapu Hari. AU - Mogami, Haruta. AU - Word, Ruth Ann. PY - 2016/6/1. Y1 - 2016/6/1. N2 - Context: Nrf2 is a key transcription factor that modulates cell defense mechanisms against endogenous and exogenous stress. Previously, we reported that thrombin increased matrix metalloproteinases and prostaglandin synthesis in human amnion mesenchymal cells. Objective: We sought to determine whether activation of Nrf2 alters the effect of thrombin on prostaglandin synthesis, protease activation, and cytokine release in human amnion. Furthermore, we analyzed the effect of Nrf2 activation on thrombin-induced preterm labor in mice. Design: Primary human amnion mesenchymal cells and pregnant mice were employed to investigate the effect of Nrf2 on thrombin-induced inflammation and preterm birth. Setting: This was a laboratory-based study ...

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1. Norwitz ER, Starkey PM, Lopez Bernal A. Prostaglandin D2 production by term human decidua: cellular origins defined using flow cytometry. Obstet. Gynecol. 1992;80:440-5 2. Nancy P, Erlebacher A. T cell behavior at the maternal-fetal interface. Int.J.Dev.Biol. 2014;58:189-98 3. Romero R, Munoz H, Gomez R, Parra M, Polanco M, Valverde V. et al. Increase in prostaglandin bioavailability precedes the onset of human parturition. Prostaglandins Leukot. Essent. Fatty Acids. 1996;54:187-91 4. Olson DM, Mijovic JE, Sadowsky DW. Control of human parturition. Semin. Perinatol. 1995;19:52-63 5. Gibb W. The role of prostaglandins in human parturition. Ann. Med. 1998;30:235-41 6. Ledingham MA, Thomson AJ, Greer IA, Norman JE. Nitric oxide in parturition. BJOG. 2000;107:581-93 7. Yallampalli C, Dong YL, Gangula PR, Fang L. Role and regulation of nitric oxide in the uterus during pregnancy and parturition. J. Soc. Gynecol. Investig. 1998;5:58-67 8. Goldenberg RL, Hauth JC, Andrews WW. Intrauterine infection ...

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Purpose: : To evaluate the efficacy of early sutureless amniotic membrane transplantation in the management of severe bacterial keratitis to reduce pain, inflammation, and haze, and to promote healing. Methods: : A non-comparative case series including 3 eyes of 3 consecutive patients with severe bacterial keratitis exhibiting persistent epithelial defect/ulcer were retrospectively reviewed following early sutureless amniotic membrane transplantation via ProKeraTM together with selective topical antibiotics and preservative-free steroid. Pain relief, inflammation, haze, and corneal epithelial healing were monitored. Results: : ProKeraTM was inserted once in one eye, twice in the other two eyes. Pain was significantly relieved, and inflammation was markedly reduced in all cases. The corneal epithelial defect and stromal ulceration rapidly healed while visual acuity improved in two out of the 3 eyes. Conclusions: : Temporary sutureless amniotic membrane transplantation via ProKeraTM allows easy ...

TY - JOUR. T1 - Evidence for active acetylcholine metabolism in human amniotic epithelial cells. T2 - Applicable to intracerebral allografting for neurologic disease. AU - Sakuragawa, Norio. AU - Misawa, Hidemi. AU - Ohsugi, Keiko. AU - Kakishita, Kouji. AU - Ishii, Takashi. AU - Thangavel, Ramasamy. AU - Tohyama, Jun. AU - Elwan, Mohamed. AU - Yokoyama, Yasunobu. AU - Okuda, Osamu. AU - Arai, Hajime. AU - Ogino, Ikuko. AU - Sato, Kiyoshi. PY - 1997/8/22. Y1 - 1997/8/22. N2 - Human amniotic epithelial (HAE) cells have been used for allotransplantation in patients with lysosomal storage disease due to lack of expression of HLA antigens. Previously, we have reported the expression of differentiation markers for both neural stem cells, and neuron and glial cells. In the present study, we investigated the presence of choline acetyltransferase (CHAT) and acetylcholine (ACh) in HAE cells using different experimental approaches. Cultured HAE cells showed strong immunoreactivity against ChAT antibody. ...

Human Amniotic Epithelial Cell Lysate https://www.sciencepro.com.br/produtos/sc-7116 https://www.sciencepro.com.br/@@site-logo/logo-novo.png ...

TY - JOUR. T1 - Inherent propensity of amnion-derived mesenchymal stem cells towards endothelial lineage. T2 - Vascularization from an avascular tissue. AU - Warrier, S.. AU - Haridas, N.. AU - Bhonde, R.. PY - 2012/10. Y1 - 2012/10. N2 - One of the most pressing problems in injury is wound healing and blood vessel formation. The amniotic membrane is important in clinical applications as it is pro-angiogenic, anti-fibrotic and anti-scarring and has low immunogenicity. In this study, we characterized amniotic membrane mesenchymal stem cells (AMMSCs) by their trademark mesenchymal stem cell (MSC) signature and profiled for embryonic pluripotency markers namely alkaline phosphatase, Oct4, Sox2, Nanog, SSEA3 and 4, and Klf4 by RT-PCR and nuclear localization of Oct4 and Nanog by immunocytochemistry. The amnion, although avascular, contains pro-angiogenic factors such as type I, III, IV and V collagen, laminin, and fibronectin in the extra cellular matrix. We, therefore, hypothesized that AMMSCs is ...

Purpose: : To examine the characteristics of iris pigment epithelial (IPE) cells cultured on amniotic membrane (AM) and to determine whether subretinal transplantation of this IPE cell sheet can protect photoreceptor cell loss in dystrophic Royal College of Surgeons (RCS) rats. Methods: : Human and rat IPE cells were cultured on the basement membrane side of dispase treated AM. After 2 weeks of seeding, the expression of several genes participate in the function of differentiated retinal pigment epithelial (RPE) cells was evaluated using real time PCR and Western blotting. Ultrastructural changes were evaluated using transmission electron microscopy. The IPE cell sheet cultured on AM was transplanted into the subretinal space of 4-week-old RCS rats, and the eyes were analyzed histologically at 12 weeks after grafting. The effect of transplantation of IPE cell sheet on the visual function was estimated by optokinetic reflex. Results: : IPE cells cultured on AM exhibited ultrastructural epithelial ...

TY - JOUR. T1 - The effects of thrombin and cytokines upon the biomechanics and remodeling of isolated amnion membrane, in vitro. AU - Kumar, D.. AU - Schatz, F.. AU - Moore, R. M.. AU - Mercer, B. M.. AU - Rangaswamy, N.. AU - Mansour, J. M.. AU - Lockwood, C. J.. AU - Moore, J. J.. PY - 2011/3. Y1 - 2011/3. N2 - Abruption-induced thrombin generation and inflammation/infection induced cytokine production have both been associated with fetal membrane (FM) weakening and preterm premature rupture of the fetal membranes (PPROM). Using our in vitro model system we have demonstrated that thrombin, and separately the cytokines, tumor necrosis factor-alpha (TNFα) and interleukin-1-beta (IL-1β), remodel and weaken full thickness FM. Additionally, we have reported that the anti-oxidant and NFκB inhibitor, alpha-lipoic acid (LA), blocks these thrombin and cytokine induced effects. The purpose of these studies was to determine whether thrombin and cytokines directly weaken the amnion membrane (AM), the ...

TY - JOUR. T1 - Denuded human amniotic membrane seeding bone marrow stromal cells as an effective composite matrix stimulates axonal outgrowth of rat neural cortical cells in vitro. AU - Liang, Hong Sheng. AU - Liang, Peng. AU - Xu, Ye. AU - Wu, Jia Ning. AU - Liang, Tao. AU - Xu, Xiao Ping. AU - Liu, En Zhong. PY - 2009/9. Y1 - 2009/9. N2 - Background: Previous studies have shown that axonal outgrowth in the damaged central nervous system is closely related to the local microenvironment. Transplantation of bone marrow stromal cells (BMSC) or BMSC with some biomaterials has been used to treat various central nervous system diseases with some success. In the current study, we investigated if BMSC on denuded human amniotic membrane (DhAM) as a composite matrix could stimulate axonal outgrowth or not. Method: After completely removing the cells on the amniotic membrane with a tryptic and mechanical approach, we seeded BMSC on it. The MTS was applied to test the cytotoxicity of DhAM compared with ...

Amniotic membrane, or amnion, is the innermost layer of the placenta and consists of a thick basement membrane and an avascular stromal matrix. Amniotic membrane transplantation has been used as a graft or as a dressing in different surgical subspecialties. In the field of ophthalmology, it is used broadly 1) to reconstruct the ocular surface after various procedures; 2) as a graft for ocular surface melts; and 3) as a bandage to promote healing in cases of persistent epithelial defects or ocular surface inflammation. All of these indications make use of amniotic membranes ability to promote healing.[1]

Amniotic membrane graft to conjunctival flap in treatment of non-viral resistant infectious keratitis: a randomised clinical study ...

Objective: To evaluate the use of hypothermically stored human amniotic membrane for cartilage repair in adult sheep. Studies show that human amniotic membrane contains pluripotent mesenchymal stem cells that can be influenced to produce chondrocytes. It is unknown if human amniotic cells can produce hyaline-like cartilage. This study evaluates the use of hypothermically stored amniotic membrane (HSAM) to fill chondral defects in a sheep model. We hypothesized HSAM would fill defects with hyaline-like cartilage with chondrocytes populating the matrix. One sheep was used as a control, and four sheep received amniotic membrane. Two of these sheep were used as a normal control comparison. A 1 cm2 defect was created on the trochlear grove in all specimens. Each membrane was sized and laid over with the stromal layer facing the subchondral bone and covered with Fibrin sealant. The knees were harvested at five months and underwent morphological, histological, and immunohistological evaluation based on the

1. John, T. Human amniotic membrane transplantation: past, present, and future. Ophthalmol Clin North Am. 2003;16(1):43-65. 2. Davis JW. Skin transplantation with a review of 550 cases at the Johns Hopkins Hospital. Johns Hopkins Med J. 1910;15:307-396. 3. Sabella N. Use of fetal membranes in skin grafting. Med Records NY. 1913;83:478-480. 4. Stern M. The grafting of preserved amniotic membrane to burned and ulcerated surfaces, substituting skin grafts. JAMA. 1913;60(13):973-974. 5. De Roth A. Plastic repair of conjunctival defects with fetal membranes. Arch Ophthalmol. 1940;23(3):522-525. 6. Internal records, Surgical Biologics, Inc, Kennesaw, GA. Accessed August 23, 2012. 7. Cornwell KG, Landsman A, James KS. Extracellular matrix biomaterials for soft tissue repair. Clin Podiatr Med Surg. 2009;26(4):507-523. 8. Bose B. Burn wound dressing with human amniotic membrane. Ann R Coll Surg Engl. 1979;61(6):444-447. 9. Quinby WC, Hoover HC, Scheflan M, et al. Clinical trials of amniotic membranes in ...

TY - JOUR. T1 - Paradox of glucocorticoid-induced cytosolic phospholipase A2 group IVA messenger RNA expression involves glucocorticoid receptor binding to the promoter in human amnion fibroblasts. AU - Guo, Chunming. AU - Yang, Zhen. AU - Li, Wenjiao. AU - Zhu, Ping. AU - Myatt, Leslie. AU - Sun, Kang. PY - 2008/1. Y1 - 2008/1. N2 - Glucocorticoids (GCs) are well-known anti-inflammatory drugs inhibiting prostaglandin production. Paradoxically, GCs are reported to stimulate cytosolic phosphoplipase A2 group IVA (PLA2G4A) and prostaglandin-endoperoxide synthase 2 (PTGS2) expression in human amnion fibroblasts. This study was designed to examine the molecular mechanisms underlying glucocorticoid-induced PLA2G4A expression in human amnion fibroblasts. Our data showed that cortisol (0.01∼1 μM) increased PLA2G4A mRNA level in a dose-dependent manner in human amnion fibroblasts, which was blocked by glucocorticoid receptor antagonist RU486 (1 μM) as well as by the mRNA transcription inhibitor ...

A reconstituted collagen membrane from human amnion has been investigated as a source of collagen matrix, which could be used as a substratum for culturing human fibroblasts. The suitability of pepsin-solubilized reconstituted human amniotic membrane, before and after cross-linking with chitosan, as a dermal matrix for culturing fibroblast was assessed by morphologic, physicochemical, cytotoxic and histochemical methods. Measurement of thermodynamic behaviour, by differential scanning calorimetric (DSC) and thermogravimetric analysis (TGA), and tensile strength suggested that the cross-linked membrane had sufficient elasticity to serve as an efficient dermal substrate for in vitro culture of fibroblasts. Fibroblasts cultured on the chitosan cross-linked collagen membrane had good adherence, retaining their morphology as indicated by microscopic analysis. Proliferation of fibroblasts, observed on this membrane affirms its non-toxic nature. These results support the application of reconstituted ...

Increasing evidence has shown that mesenchymal stem cells (MSCs) yield a favorable therapeutic benefit for thermal burn skin wounds. Human amniotic MSCs (hAMSCs) derived from amniotic membrane have multilineage differentiation, immunosuppressive, and anti-inflammatory potential which makes them suitable for treating skin wounds. However, the exact effects of hAMSCs on the healing of thermal burn skin wounds and their potential mechanisms are not explored. hAMSCs were isolated from amniotic membrane and characterized by RT-PCR, flow cytometry, immunofluorescence, and tumorigenicity test. We assessed the effects of hAMSCs and hAMSC conditional medium (CM) on wound healing in a deep second-degree burn injury model of mice. We then investigated the biological effects of hAMSCs and hAMSC-CM on the apoptosis and proliferation of heat stress-injured human keratinocytes HaCAT and dermal fibroblasts (DFL) both in vivo and in vitro. Next, we explored the underlying mechanisms by assessing PI3K/AKT and GSK3β/β

TY - GEN. T1 - Prediction of stem cell differentiation in human amniotic membrane images using machine learning. AU - Obritzberger, Lisa. AU - Borgmann, Daniela. AU - Schaller, Susanne. AU - Dorfer, Viktoria. AU - Lindenmair, Andrea. AU - Wolbank, Susanne. AU - Hennerbichler, Simone. AU - Redl, Heinz. AU - Winkler, Stephan. PY - 2015. Y1 - 2015. N2 - It has been shown that it is possible to differentiate viable amniotic membrane towards osteogenic lineage, i.e. bony tissue. This process of mineralization may take several weeks and can show different manifestations per sample. The tissue can only be used, when the mineralization process is advanced in a certain degree. Therefore, a forecast of the development of mineralization would be helpful to save time and resources. This paper shows how a prediction on the development of mineralization can be made by using several image processing techniques, machine learning methods, and hybrid ensembles of machine learning algorithms.. AB - It has been ...

References: 1. Niknejad H, Peirovi H, Jorjani M, et al. Properties of the Amniotic Membrane For Potential Use in Tissue Engineering. European Cells and Materials. 2008(15):88-89. 2. Gruss J, Jirsch D. Human amniotic membrane: a versatile wound dressing. CMA Journal.1978(18);1237-1254. 3. Data on File, Nutech Medical. 4. Diaz-Prado A, Rendal-Vasques E, Muinos-Lopez E, et al. Potential use of the human amniotic membrane as a scaffold in articular cartilage repair. Cell Tissue Bank. 2010;11:183-195. 5. Garcia D, Longo U, Vaquero J, Forriol F, Loppini M, Khan W, Denaro V. Amniotic Membrane Transplant for Articular Cartilage Repair: An Experimental Study in Sheep. Current Stem Cell Research & Therapy. 2014;9(6);1-7. 6. Cooke, M, Tan, E, Mandrycky, C, He, H, OConnell, J, Tseng, S, Comparison Of Cryopreserved Amniotic Membrane And Umbilical Cord Tissue With Dehydrated Amniotic Membrane/Chorion Tissue, Journal Of Wound Care 2014, 23(10): 465-76.. Document No.: Affinity-1101 Revision: Initial Release ...

Synonyms for amnions in Free Thesaurus. Antonyms for amnions. 2 synonyms for amnion: amnios, amniotic sac. What are synonyms for amnions?

Objective: The aim of this research was to present the results of patients with the indication for treatment of cornea by amniotic membrane transplant over a four-year period, from 2012 to 2015. Study design: Retrospective cohort study. Participants and methods: The study included patients with pathological changes in the cornea, who have undergone amniotic membrane transplantation over the four-year period from 2012 to 2015, at the Department of Ophthalmology, University Hospital Center Osijek. The data for each patient were taken on the following: the indication for the amniotic membrane transplant, clinical findings on the cornea and visual acuity before and after the procedure, the possible reoperation, as well as the demographic characteristics. Results: Out of the total of 11 patients, 9 were women. Inflammation was the most common cause of clinical findings, in 5 patients. Infection was not found in 8 patients. Two patients complained about postoperative pain. Subjective improvement month ...

Wound healing is a complex pathophysiological process that involves a variety of cells and cytokines. In this study, we found that local injection of human amnion mesenchymal stem cells into wounds in rats could promote wound healing. Therefore, we hypothesized that the exosomes of human amnion mesenchymal stem cells contain substances that regulate the migration of epidermal cells. It has been reported that miR-135a is involved in cell migration and transformation. However, there have been no reports of its function in skin wound healing. To test this hypothesis, we injected exosomes overexpressing miR-135a directly into the wound margin. In addition, we tested the migration of BJ cells with overexpression or knockdown of miR-135a in vitro. Additionally, Western blot analysis was used to detect the expression of fibroblast migration-associated proteins after treatment with miR-135a overexpression or knockdown. MiR-135a significantly promoted wound healing compared to the control treatment. Western blot

Results Culture-expanded amnion-derived adherent cells expressed mesenchymal stem cell markers and HLA-ABC molecules and could differentiate into osteoblasts, adipocytes and chondrocyte-like cells, indicating that the cells have the characteristics of mesenchymal stem cells. The Lin-CD34+ cells purified from the frozen umbilical cord blood were strongly positive for HLA-ABC, and contained a large number of hematopoietic stem cells. When the Lin-CD34+ cells were cultured on the autologous (MHC-matched) or MHC-mismatched amnion-derived adherent cells in short-term assays (hematopoietic stem cell-proliferation) and long-term culture-initiating cell assays, greater expansion of the Lin-CD34+ cells was observed in the MHC-matched combination than in MHC-mismatched combinations. The concentration of granulocyte-macrophage colony-stimulating factor in the culture supernatants of the long-term culture-initiating cell assays was significantly higher in the MHC-matched combination than in MHC-mismatched ...

619PRTHuman Immunodeficiency Virus 1Arg Lys Lys Arg Arg Gln Arg Arg Arg1 529PRTHuman Immunodeficiency Virus 2Lys Lys Lys Lys Lys Lys Lys Lys Lys1 539PRTHuman Immunodeficiency Virus 3Arg Arg Arg Arg Arg Arg Arg Arg Arg1 54281PRTHomo sapiens 4Met Asn Gly Glu Glu Gln Tyr Tyr Ala Ala Thr Gln Leu Tyr Lys Asp1 5 10 15Pro Cys Ala Phe Gln Arg Gly Pro Ala Pro Glu Phe Ser Ala Ser Pro 20 25 30Pro Ala Cys Leu Tyr Met Gly Arg Gln Pro Pro Pro Pro Pro Pro His 35 40 45Pro Phe Pro Gly Ala Leu Gly Ala Glu Gln Gly Ser Pro Pro Asp Ile 50 55 60Ser Pro Tyr Glu Val Pro Pro Leu Ala Asp Asp Pro Ala Val Ala His65 70 75 80Leu His His His Leu Pro Ala Gln Leu Ala Leu Pro His Pro Pro Ala 85 90 95Gly Pro Phe Pro Glu Gly Ala Glu Pro Gly Val Leu Glu Glu Asn Arg 100 105 110Val Gln Leu Pro Phe Pro Trp Met Lys Ser Thr Lys Ala His Ala Trp 115 120 125Lys Gly Gln Trp Ala Gly Gly Ala Tyr Ala Ala Glu Pro Glu Glu Asn 130 135 140Lys Arg Thr Arg Thr Ala Tyr Thr Arg Ala Gln Leu Leu Glu Leu Glu145 150 155 160Lys Glu Phe Leu Phe Asn Lys Tyr ...

TY - JOUR. T1 - Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities. AU - Parolini, Ornella. AU - Magatti, Marta. AU - De Munari, Silvia. AU - Vertua, Elsa. AU - Gibelli, Lucia. AU - Wengler, Georg S.. PY - 2008. Y1 - 2008. N2 - Cells derived from the amniotic membrane of human placenta have been receiving particular attention because of their stem cell potentiality and immunomodulatory properties, which make them an attractive candidate source for cell therapy approaches. In this study, we isolated cells from the mesenchymal region of amnion and identified two subpopulations discordant for expression of the HLA-DR, CD45, CD14, and CD86 cellular markers. We therefore refer to the unfractionated cell population derived from this region as amniotic mesenchymal tissue cells (AMTC). We studied the suppressive and stimulatory characteristics of the unfractionated, HLA-DR-positive, and HLA-DR-negative AMTC populations and demonstrated that all three ...

Biobusiness Grant Recipient: John Schorgl, BA. Grant Period: 2017-2018. Award Value: $100,000. Site: Peytant Solutions, Inc.. Project Summary:. Peytant Solutions is developing a human amnion tissue cover scaffold, called the AmnioStent™, for the treatment of malignant airway strictures. The goal of this grant is to conduct sterilization and biocompatibility validation testing of AmnioStent per the FDA Guidance for Premarket Notifications of Esophageal and Tracheal Prostheses and preparation of the FDA 510k application. ...

TY - JOUR. T1 - Fetal rabbit gastric epithelial cells cultured on floating collagen gels. AU - Logsdon, C. D.. AU - Bisbee, C. A.. AU - Rutten, M. J.. AU - Machen, T. E.. PY - 1982/3. Y1 - 1982/3. N2 - Cells were isolated from ∼ 30 d fetal rabbit stomachs and cultured on floating collagen gels. Electron microscopy showed monolayers in which only one cell type persisted. These columnar cells were joined at apical borders by tight junctions and contained an extensive endoplasmic reticular network with an occasional intracellular canaliculus. They also occasionally contained what appeared to be secretory granules (mucus?), and therefore had some characteristics of all the cell types of the intact fetal stomachs, which showed oxyntic, mucous, and undifferentiated cells. In Ussing chambers with Ringers solution on both sides, cultures developed transepithelial potential (potential difference [PD], mV, mucosa ground)=13, resistance (resistance [R], Ω-cm2)=285, and short-circuit current (Isc, ...

c. chorion and amnion. recent questions recent answers. Another form of this procedure is incision and drainage also known as clinical lancing which is a procedure used to drain the pus from an infected portion of the body. b. amnion. ANS: A REF: 326 OBJ: 3 TOP: Neonatology Disease and Disorder Terms Built from Word Parts 27. Amniocentesis is the medical term meaning surgical puncture of the amnion. According to Dictionary.com, the term cervix refers to the neck or any neck-like part of an organ. Traditional sutures or staples, however, are usually removed after about a week or two. Q 75 . J Am Geriatr Soc. Sign up here. The midline incision is the preferred incision due to adequate exposure of intra-abdominal contents, quick access, and relative ease to perform.1-3 Laparotomies are associated with a number of complications like wound dehiscence, wound infection and incisional hernias.1,4,5 Midline laparotomy incision is generally chosen by surgeons because of its ease, speed and excellent ...

Maternal immunoglobulin-G (IgG) is known to be transported across the placental syncytiotrophoblast during the period when the human fetus is incapable of manufacturing these defensive molecules. In this study we investigated the possible role of the amniochorion, that surrounds the amniotic cavity in which the fetus lies, in the transfer of immunoglobulin. Endogenous IgG was localised in the amniochorion by confocal immunofluorescence microscopy and by ultrastructural labelling of ultrathin frozen tissue sections using the protein A-gold technique. Immunoreactivity was identified in the extracellular matrix tissues and necrotic amniotic epithelial cells. Healthy amniotic epithelial cells and cytotrophoblast cells of the chorion laeve were devoid of endogenous IgG. These results suggest a possible nonspecific paracellular transport pathway between cytotrophoblast cells, which may conceivably contribute to the acquisition of passive immunity by the fetus, and offer a rational explanation for the ...

Shortly after the establishment of the circulation of the yolk-sac the folds of the amnion meet and coalesce above the embryo (Fig. 114, 3 and 4, am). After this the inner or true amnion becomes severed from the outer or false amnion, though the two sometimes remain connected by a narrow stalk. The space between the true and false amnion is a continuation of the body cavity. The true amnion consists of a layer of epiblastic epithelium and generally also of somatic mesoblast, while the false amnion consists as a rule of epiblast only; though it is possible that in some cases (the rabbit ?) the mesoblast may be continued along its inner face. Before the two limbs of the amnion are completely severed the epiblast of the umbilical vesicle becomes separated from the subjacent mesoblast and hypoblast of the vesicle (Fig. 114, 3), and, together with the false amnion (sh) with which it is continuous, forms a complete lining for the inner face of the zona radiata. The space between this membrane and the ...

Human amnion as a temporary biologic barrier after hysteroscopic lysis of severe intrauterine adhesions pilot study J Minim Invasive Gynecol. 2010 Sep-Oct;17(5):605-11. doi: [...] ...

Preterm White Matter Brain Injury Is Prevented by Early Administration of Umbilical Cord Blood Cells Researchers assessed the neuroprotective effects of umbilical cord blood cells and optimal administration timing in a fetal sheep model of preterm brain injury. [Exp Neurol] Abstract Amnion-Epithelial-Cell-Derived Exosomes Demonstrate Physiologic State of Cell under Oxidative Stress Investigators determined amnion epithelial cells (AECs) exosome characteristics and their cargo reflected the physiologic status of the cell of origin and suggests that AEC-derived exosomal p38 MAPK plays a major role in determining the fate of pregnancy. [PLoS One] Full Article Reconstitution of Immune Cell in Liver and Lymph Node of Adult- and Newborn-Engrafted Humanized Mice The authors compared reconstitution levels in a variety of immunological tissues, including the lymph node and liver, between mice engrafted intravenously as adults and intrahepatically in newborns. CD34+ cells were enriched from cord blood and ...

2. A Ocular surface reconstruction with allolimbal transplantation and use of two membranes. The inner 9-mm disc acts as a graft and the outer larger membrane as a patch. S. Dua). B The outer membrane has cut through sutures and retracted, exposing the inner membrane. e. 5 Clinical Studies and Outcomes (Definitions of Success and Grading of Disease Severity) A B membrane was intended as a patch and acted as such for the expected duration but the PED did not heal. When the above criteria were applied to 74 procedures involving use of the amniotic membrane, failure of the procedure was observed in 44 % of patients where the membrane was used in the presence of stem cell deficiency, in 33 % of procedures where the membrane was used in the absence of stem cell deficiency and in 44 % of patients where the membrane was used for conjunctival reconstruction. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. References 1. Anderson DF, Ellies P, Pires RT, Tseng SC (2001) Amniotic membrane transplantation for partial ...

Moreover, the reduced proportion of Treg cells in EAU rats was restored upon hAEC treatment during disease development (Fig. therapeutic strategy is urgently required. The present study investigated the therapeutic efficacy of human amniotic epithelial cells (hAECs) on autoimmune uveitis in a rat model. Herein, experimental autoimmune uveitis (EAU) was induced in rats via a subcutaneous injection of interphotoreceptor retinoid-binding protein. EAU rats were treated with hAECs or the vehicle solution via a subretinal injection on day 0 and day 6 after immunization, and rats were sacrificed on day 12 and day 18 for further analysis. The pathological development of EAU was evaluated by slit lamp microscopy. Immune cell infiltration and retinal structure damage were examined by histological examination of hematoxylin TGR-1202 and eosin (H&E) and immunofluorescence staining. T-cell subsets were detected by flow cytometry, and the levels of inflammatory cytokines were quantified by enzyme-linked ...

The basic helix-loop-helix (bHLH) transcription factor, Hand1, plays an important role in the development of the murine extra-embryonic trophoblast cell lineage. In the present study, we have analysed the expression of Hand1 in human extra-embryonic cell types and determined its binding specificity and transcriptional activity upon interaction with different class A bHLH factors. Northern blotting and in situ hybridization showed that Hand1 mRNA is specifically expressed in amnion cells at different stages of gestation. Accordingly, we demonstrate that the protein is exclusively produced in the amniotic epithelium in vivo and in purified amnion cells in vitro using a novel polyclonal Hand1 antiserum. Reverse transcriptase-PCR and immunohistochemical staining of blastocysts revealed the production of Hand1 mRNA and polypeptide in the trophectodermal cell layer. In the presence of E12/E47, Hand1 stimulated the transcription of luciferase reporters harbouring degenerate E-boxes, suggesting that ...

Amnion: Amnion,, in reptiles, birds, and mammals, a membrane forming a fluid-filled cavity (the amniotic sac) that encloses the embryo. The amniotic sac and the fluid it contains are

Abstract: The purpose of this study was to investigate the relationship between cell apoptosis and wide-band electromagnetic pulse (WEMP) irradiation parameters and to provide experimental data to establish the biophysics damage model for WEMP. Cell counting kit-8 was used to detect the cell viability changes after WEMP irradiation and flow cytometry assay was used to detect cell apoptosis after irradiation under different WEMP irradiation parameters. Under experimental conditions, field strength and number of pulses were observed to be the main factors that increased the apoptotic rate of human amniotic epithelial cells. Specifically, electric field intensity and number of pulses were determined to be the primary and secondary factors, respectively. The two factors were confirmed to interact with each other, that is, a logistic function was found to exist between the apoptotic rate and electric field strength.. Key words: Wide-band electromagnetic pulse,Apoptosis,Variance analysisCLC, TL71, ...

TY - JOUR. T1 - Fine structure and immunofluorescent studies of the wish cell line. AU - Meek, William D.. AU - Davis, Walter L.. PY - 1986/12/1. Y1 - 1986/12/1. N2 - Morphological studies of the WISH cell line reveal an epithelioid cell type with some characteristics of both the original human amnion epithelium and a transformed state. WISH cells have a cytoplasm filled with microtubules; however, actin filament bundles are few, with actin localized at areas of cell contact and arranged diffusely through the cytoplasm, as viewed by indirect immunofluorescence. Fingerlike projections or short filopodia are observed connecting cells that grow in a closely apposed monolayer. Other surface features, as viewed by scanning electron microscopy, include microvilli and blebs. Transmission electron microscopy shows that WISH cultures consist of light or dark cells with organelles that include lipid droplets, abundant free ribosomes, tubular mitochondria, lysosomes, annulate lamellae, rough endoplasmic ...

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Placenta, Cord, Amnion, Chorion Model - $89 A great teaching tool, this model of the placenta, cord, amnion, and chorion is perfect for childbirth education courses. Visit Cascade HealthCare Products secure Website to view our complete selection of educational materials for childbirth educators and professionals or to place an order.

ABSTRACT BACKGROUND: The human placenta-derived amniotic mesenchymal stem cells (hAMSCs) exhibit immunomodulatory, partially pluripotent, and precardiac properties. They express the c-kit cardiac progenitor marker and are readily reprogrammed to iPSCs (MiPSCs). Dual contrast manganese-enhanced MRI (MEMRI) and delayed enhancement MRI (DEMRI) has been developed to evaluate the cardiac restorative effects of hAMSCs, c-kit+ hAMSCs, and MiPSCs.. HYPOTHESIS: MEMRI and DEMRI will identify the mechanism of myocardial restoration.. METHODS: SCID-beige mice underwent LAD ligation and received 250,000 cells or normal saline (NS) in the peri-infarct region. The mice were divided into 4 arms: (1) NS (control group, n=7), (2) hAMSC (n=6), (3) c-kit+ hAMSC group (n=2), and (4) MiPSC group (n=3). The hAMSCs were transfected with a luciferase reporter gene. Cardiac MRI measured the scar, viable myocardium, and LVEF at weeks 1, 2, and 4. Bioluminescence imaging (BLI) was also performed and blood was drawn at week ...

One of the most established roles of oxytocin (OT) is in inducing uterine contractions and labour. Apart from contractions, our recent study have shown that OT can also activate pro-inflammatory pathways in both human myometrial and amnion cells which suggests the pro-inflammatory role of OT should be taken into account when developing tocolytics targeting the OT/OT receptor (OT/OTR) system. The OTR antagonist, Atosiban, is currently used therapeutically for the treatment of PTL. We have previously shown that Atosiban fails to inhibit the pro-inflammatory effects of OT in human amnion, and Atosiban alone activates NF-κB and MAPKs thus upregulating downstream pro-labour genes. In contrast to our findings with Atosiban, the presence of the orally active OTR antagonist, Nolasiban, reduced the effect of OT on NF-κB and p38 kinase activation both in myometrial and amnion cells. Consistent with the activation of these inflammatory mediators, OT led to increases in the expression of COX-2 and ...

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Define amnion: a thin membrane forming a closed sac about the embryos or fetuses of reptiles, birds, and mammals and containing the amniotic fluid

The amniotic sac, commonly called the bag of waters, sometimes the membranes, is the sac in which the fetus develops in amniotes. It is a thin but tough transparent pair of membranes that hold a developing embryo (and later fetus) until shortly before birth. The inner of these fetal membranes, the amnion, encloses the amniotic cavity, containing the amniotic fluid and the fetus. The outer membrane, the chorion, contains the amnion and is part of the placenta. On the outer side, the amniotic sac is connected to the yolk sac, the allantois and, via the umbilical cord, to the placenta. Amniocentesis is a medical procedure where fluid from the sac is sampled to be used in prenatal diagnosis of chromosomal abnormalities and fetal infections. The amniotic cavity is the closed sac between the embryo and the amnion, containing the amniotic fluid. The amniotic cavity is formed by the fusion of the parts of the amniotic fold, which first makes its appearance at the cephalic extremity, and subsequently at ...

Research supported by The Eye Cancer Foundation has proved greater efficacy of this new technique in recreating the outer surface of the eye and inner surface of the eyelids. As published in the American Journal of Ophthalmology on November 2018, tumors of the conjunctiva and eyelids were surgically removed, then amniotic membranes from donor human placentas were sewn into the defects to recreate a normal ocular and inner eyelid surface. Thus, amnion can provide a foundational platform for new cells to grow and flourish. In this case series, super thick amniotic membrane grafts (AMGs) were found to facilitate the healing of wounds. How exactly does graft thickness affect the success of treatment? Well, the greater thickness means it is more easily sewn into the affected area, and. also helps the grafts to remain several weeks after placement. Thicker grafts are less likely to tear, rupture, or dissolve during the postoperative period. Most importantly, following treatment with ST-AMG, every ...

Human amniotic membrane is a unique collagenous tissue derived from the innermost submucosa of the placenta, the area in which the human fetus grows and develops within the mothers uterus. Human amniotic membrane is composed of a single epithelium layer, a thick basement membrane, and an avascular stromal matrix.. ...

Looking for online definition of amniotic epithelium in the Medical Dictionary? amniotic epithelium explanation free. What is amniotic epithelium? Meaning of amniotic epithelium medical term. What does amniotic epithelium mean?

In this study we aimed to set up an in vitro culture of the rabbit amnion in order to support in vivo fetal membrane healing capacity following fetoscopy. Fetal membranes were collected from a mid- gestational rabbit, and cultured on collagen support material for 14 days. 34 rabbits at 22 - 23 days gestational age ( GA) underwent fetoscopy. The entry site was randomly allocated to 4 closure technique study groups: group I, human amnion membrane ( n = 23); group II, collagen foil ( n = 16); group III, collagen plug ( n = 19), and group IV, collagen plug with cultured amnion cells ( n = 19). In all groups membrane access sites were additionally sealed with fibrin sealant, and the myometrium was closed with sutures. Fetal survival, amnion membrane integrity, and the presence of amniotic fluid were evaluated at 30 days GA. Cultures showed good survival in the collagen support material. Increased cellularity, survival and proliferations were observed. The amnion at the access site resealed in 58 - ...

Ocular surface is composed of corneal and conjunctival epithelial cells. Normal cornea is essential for normal vision. Defects in renewal and repair of ocular surface as a result of limbal stem cell deficiency are now known to cause varying ocular surface morbidity including persistent photophobia, repeated and persistent surface breakdown and overt conjunctivalisation of the cornea. Restoring ocular health in these eyes has traditionally been frustrating. Ex vivo cultured limbal epithelial stem cells have been used successfully to treat limbal stem cell deficiency. Ex-vivo limbal stem cell allograft transplantation is achieved by harvesting limbal corneal tissue from donor eyes (either matched living relatives or cadaveric donors). The donor stem cells are obtained by excising a small area of the conjunctiva at the limbus and are a minor procedure. The tissue so obtained is then grown in tissue culture and once the cells have multiplied sufficiently, small sheets are transplanted on to the ...

TY - JOUR. T1 - Inhibition of lysyl oxidase by cortisol regeneration in human amnion. T2 - Implications for rupture of fetal membranes. AU - Liu, Chao. AU - Guo, Chunming. AU - Wang, Wangsheng. AU - Zhu, Ping. AU - Li, Wenjiao. AU - Mi, Yabing. AU - Myatt, Leslie. AU - Sun, Kang. N1 - Funding Information: This work was supported by National Natural Science Foundation of China Grants 81330018 and 81270704 and National Key Basic Research Program of China Grant 2014CB943302. Disclosure Summary: The authors have no conflict of interest to disclose. Publisher Copyright: Copyright © 2016 by the Endocrine Society.. PY - 2016. Y1 - 2016. N2 - The mechanisms underlying human parturition are still not understood, yet we need this knowledge to combat preterm birth. Fetal membranes express abundant 11 β-hydroxysteroid dehydrogenase 1 (11β-HSD1), which converts inert cortisone to active cortisol. We examined whether cortisol regeneration in the amnion might play a role in human parturition through ...

TY - JOUR. T1 - Vesicular uptake of macromolecules by human placental amniotic epithelial cells. AU - Sharshiner, Rita. AU - Brace, Robert A.. AU - Cheung, Cecilia Y.. N1 - Funding Information: This work was supported by NIH grant 5R01 HD061541 from the National Institute of Child Health and Human Development; Shared Instrumentation Grant Number S10-RR023432 from the National Center for Research Resources; Oregon Health and Science University Core Pilot Grant; and Oregon Health and Science University Division of Maternal Fetal Medicine. The contents of this study are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH/NICHD. Publisher Copyright: © 2017 Elsevier Ltd Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 2017/9. Y1 - 2017/9. N2 - Introduction Studies in animal models have shown that unidirectional vesicular transport of amniotic fluid across the amnion plays a primary role in regulating amniotic fluid volume. Our ...

Members of the Vampire Facelift® provider group, can login to the members site to see more specifics of how to use Vampire Amnion™ to rejuvenate the hands and face and to receive special pricing from the largest supplier of quality amnion in the U.S.. Over 1,000 research papers about amnion (click),-. ONLY live birth amnion is used (no aborted fetal tissue ...

Methods This retrospective study included 200 patients, above 8 years of age, with clinically diagnosed unilateral total limbal stem cell deficiency due to ocular surface burns treated between 2001 and 2010. A small limbal biopsy was obtained from the unaffected eye. The limbal epithelial cells were expanded ex vivo on human amniotic membrane for 10-14 days using a xeno-free explant culture system. The resulting cultured epithelial monolayer and amniotic membrane substrate were transplanted on to the patients affected eye. Postoperative corneal surface stability, visual improvement and complications were objectively analysed. ...

Aim. This project studies the regeneration of epidermal and dermal components with the use human Whartons Jelly Mesenchymal Cells (WJ-MSCs) on a scaffold of Freeze Dried Human Amniotic Membrane (FD-HAM) and Cryopreserved Human Amniotic Membrane (CP-HAM) in porcine excisional wound model. Methods. Fresh AM was harvested from seronegative term pregnant mothers undergoing elective Caesarean section deliveries under sterile conditions. The membrane was de-cellularised and either cryopreserved (CP-HAM) or freeze dried (FD-HAM) for storage. Commercially available WJ-MSCs were seeded on the different forms of the decellularised membrane. Six, 5×5 cm cutaneous wounds using a dermatome at 0.8mm setting, 2cm apart will be created on the dorsum of 20-25kg Great White pigs on either side of the spine under general anaesthesia. The treatments (Wound control, WJ-MSCs alone, FD-HAM, CP-HAM, FD-HAM + WJ-MSCs, CP-HAM+ WJ-MSCs) were allocated by Latin squares to randomise the application. A barrier dressing ...

Our purpose was to evaluate the diagnostic value of three-dimensional (3 D) CT surface reconstruction in spinal fractures in comparison with axial and reformatted images. A total of 50 patients with different CT-proven spinal fractures were analysed retrospectively. Based on axial scans and reformatted images, the spinal fractures were classified according to several classifications as Magerl for the thoraco-lumbar and lower cervical spine by one radiologist. Another radiologist performed 3 D CT surface reconstructions with the aim of characterizing the different types of spinal fractures. A third radiologist classified the 3 D CT surface reconstruction according to the Magerl classification. The results of the blinded reading process were compared. It was checked to see in which type and subgroup 3 D surface reconstructions were helpful. Readers one and two obtained the same results in the classification. The 3 D surface reconstruction did not yield any additional diagnostic information concerning type

Koizumi, N and Rigby, H and Fullwood, N J and Koizumi, K and Joussen, A M and Kociok, N (2004) Comparison of intact and denuded amniotic membrane as a substrate for human limbal epithelial cell culture. Investigative Ophthalmology and Visual Science, 45. U336-U336. ISSN 0146-0404 Full text not available from this repository ...

Approaches providing the positioning of human amniotic membrane (HAM) within the intra-articular space of arthritic TMJs have never been investigated. This contrasts with the increasing amount of evidence suggesting the potential positive effects of HAM on a number of surgical conditions, even included the interpositional arthroplasty for TMJ ankylosis. Thus, the possible usefulness of HAM to restore joint functions in severely damaged TMJs could be hypothesized. Based on these premises, the clinical research question Is human amniotic membrane positioning effective to reduce symptoms and restore jaw function in patients with severe inflammatory-degenerative disorders of the temporomandibular joint? has been addressed by performing a systematic review of the literature ...

Limbal stem cells were obtained by excising a small area of the conjunctiva at the limbus. The limbal tissue was exposed, for 5-10 min, to Dispase II (1.2 U/mL in Mg2+ and Ca2+-free HBSS) at 37°C in a humidified environment containing 5% CO2. The tissue was rinsed with DMEM/F12 containing FBS (10%) and cut into cubes of approximately 1-2 mm2. The limbal epithelial cells were cultured on the basement membrane side of denuded human amniotic membranes (AMs) and cultured in DMEM/Hams F-12 (1:1) supplemented with FBS (5%), DMSO (0.5%), hEGF (2 ng/mL), insulin (5 μg/mL), transferrin (5 μg/mL), selenium (5 ng/mL), hydrocortisone (0.5 μg/mL), cholera toxin (30 ng/mL), gentamicin (50 μg/mL), and amphotericin B (1.25 μg/mL). Cultures were incubated in a humidified incubator with 5% CO2. The cultures were maintained for 3 weeks and the medium was replaced every 2 days. ...

Introduction: Human amniotic membrane (AM) is the innermost layer of placenta and is composed of three distinct strata, namely single epithelial layer, a thick basement membrane and avascular stroma. AM has been widely adopted as a biomaterial in tissue engineering and surgical reconstructions, since it promotes wound healing and epithelialization, has low immunogenicity, reduces inflammation and scarring, and was also shown to possess anti-cancer properties (1). We have shown that AM promotes formation of highly differentiated urothelium, in particular when urothelial cells are cultured on AM stromal side (2). Although AM stroma scaffold allows establishment of differentiated urothelium, its orientation probably does not favour AM integration into surrounding tissue, if applied in vivo. Therefore, we established an in vitro AM model which would allow incorporation of fibroblasts into the AM stroma as well as promote urothelial differentiation.. Materials and Methods: Porcine bladder fibroblasts ...

During the pathogenesis of Lyme disease, Borrelia burgdorferi spreads hematogenously from the site of a tick bite to several tissues throughout the body. The specific mechanism of spirochete emigration is presently unknown. Using cultured human umbilical vein endothelial cells, we found that Borrelia burgdorferi bound to the endothelial cells and to the subendothelial matrix. Low passage isolates adhered 22-30-fold greater than a strain maintained in culture continuously. Spirochete binding to subendothelial matrix was inhibited 48-63% by pretreatment of the matrix with anti-fibronectin antiserum. Spirochete migration across endothelial monolayers cultured on amniotic membrane was increased when the monolayers were damaged by chemical or physical means. Electron microscopic examination of spirochete-endothelial interactions demonstrated the presence of spirochetes in the intercellular junctions between endothelial cells as well as beneath the monolayers. Scanning electron microscopy identified a ...

The gestational sac is the large cavity of fluid surrounding the embryo. During early embryogenesis it consists of the extra-embryonic coelom, also called the chorionic cavity. The gestational sac is normally contained within the uterus. It is the only available structure that can be used to determine if an intrauterine pregnancy exists until the embryo is identified. On obstetric ultrasound, the gestational sac is a dark (anechoic) space surrounded by a white (hyperechoic) rim. The gestational sac is spherical in shape, and usually located in the upper part of the fundus of the uterus. By approximately 9 weeks of gestational age, the amniotic sac has expanded to occupy the majority of the volume of the gestational sac, eventually expanding to reduce the extraembryonic coelom to a thin layer between the amnion membrane and the mesoderm. By then, the gestational sac is usually simply called the amniotic sac. During embryogenesis, the extraembryonic coelom (or chorionic cavity) that ...

The human tissue inhibitor of metalloproteinases (TIMP) is a glycoprotein with a molecular weight of 28,000. It appears to be ubiquitous in human mesoderm tissues and has previously been shown to be identical to the collagenase inhibitor isolated from human skin fibroblasts. TIMP inhibits type I- and IV-specific collagenases and other neutral metalloen-doproteinases that may be responsible for the degradation of extracellular matrix in tumor cell metastasis. In this work we have utilized recombinant human TIMP (rTIMP) obtained by expression of its cDNA gene (Carmichael et al., Proc. Natl. Acad. Sci. USA, 83: 2407, 1986). The rTIMP is shown to have similar inhibition properties as natural TIMP against human skin fibroblast collagenase. In an in vitro amnion invasion assay system, rTIMP inhibited the invasion of B16-F10 murine melanoma cells through the human amniotic membrane at an identical concentration to that reported previously for natural TIMP. The mechanism by which rTIMP inhibits amniotic ...

Patient I am an Australian whose daughter contacted Dr. Maskin as soon as we heard about him and that he was performing a procedure for extreme Dry Eye Syndrome, called Amniotic Membrane Transplantation, and then made all the arrangements to have a consultation with him within 2 and ½ weeks.. I have Sjogrens Syndrome and for 8 years I have been trying to get help and relief from the acute debilitating pain, burning irritation and discomfort of always feeling as though I have a large piece of foreign matter in my eyes, as well as though grit has been thrown into my eyes and they were being rubbed with sandpaper.. Sometimes I felt as though my left eye, particularly, was being pierced with a sharp instrument and it was being twisted to cause the maximum pain. Repeatedly, I would say to the doctors here: I cant believe that my eyes feel like this-yet you cant see anything.. I had tried every type of drops, ointment, lubricants, antibiotic drops for eyes, steroid drops and autologous ...

56 patients with age ranging from 22 to 96 years (mean: 58.6+/-20.4 years) were admitted for a period of 2 to 34 days (mean: 10+/-6.6 days). Corneal scrapes were positive in 27/54 patients. Commonly identified organisms were Staphylococcus aureus (n=7), Moraxella (n=6) Herpes Simplex Virus (n=5) and Pseudomonas (n=4). Fluoroquinolone monotherapy was used as primary treatment in 83%, which was modified in 6% based on microbial profile. Tectonic grafts were required for 4 patients; amniotic membrane transplantation and corneal glue for 2 patients each; three underwent temporary tarsorraphy; one aphakic patient required AC tap and intravitreal antibiotics; one underwent evisceration ...

Recent years have seen considerable advances in our knowledge of the biology and properties of stem/progenitor cells isolated from placental tissues. This has encouraged researchers to address the potential effects of these cells in animal models of different diseases, resulting in increasing expectations regarding their possible utility for cell-based therapeutic applications. This rapidly evolving research field is also enriched by studies aimed at expanding the use of the whole amniotic membrane (AM), a well-known surgical material, for pathological conditions other than those tested so far and for which clinical applications already exist. In this review, we provide an update on studies that have been performed with placenta-derived cells and fragments of the entire AM to validate their potential clinical applications in a variety of diseases, in particular those associated with degenerative processes induced by inflammatory and fibrotic mechanisms. We also offer, as far as possible, insight ...

Acellular amniotic membrane (AAM) is a biodegradable and biocompatible natural that has a wide range of applications in the field of pharmaceutics, biomedical, chemical and cosmetics industry. One of the most interesting characteristics of AAM is its antibacterial and anti-inflammatory, anti-angiogenic, anti-fibrosis activity is an attractive approach for treating inflammatory disorders, wounds, and burns. The isolated use of AAM may not be sufficient to produce an adequate anti-inflammatory and antimicrobial effect to fulfill different purposes, has attracted considerable attention that has similar properties to the native tissues. This mini-review offers a focuses on modern aspects of the biomaterials, growth factors, biocompatibility, and acellular matrix which are the basic elements for use in the tissue regeneration and replacement after an injury.

A HUMAN O\£UM NINE TO TEN DAYS OLD ova, the diameter of the disc would be 0.117 mm.; that of the disc in the 11.5 day Hertig-Rock ovum is o.I38 mm. At the edge of the disc the ectodermal cells suddenly change to the flattened cells comprising the side walls and roof of the amniotic cavity, a feature which supports the views of Ramsey and Heuser and Streeter that in primates the ectodermal layer of the disc is formed from the inner cell mass, whereas the amnion is derived from the mesoblast, which in its turn has been delaminated from the cytotrophoblast. In the present specimen the roof of the amnion is deficient in part ; here the cavity is roofed over by cytotrophoblast and transition cells between cytotrophoblast and amniotic cells are present. Definite yolk sac has not yet been formed. DISCUSSION AND COMPARISON wrrrr OTHER EARLY HUMAN OVA. The smaller dimensions of the chorionic vesicle and of the embryonic disc, and the lesser degree of enlargement of the endometrial sinusoids in the ...

The role of chemical potentials in surface reconstructions is examined and shown to be crucial for binary semiconductor srufaces such as GaAs(111)2×2. We predict that under As-rich conditions a new model, the As triangle, is the lowest-energy geometry, whereas the Ga-vacancy model is appropriate for Ga-rich conditions. A change in the relative chemical potential of Ga and As should produce a phase transition between the two structures. ...

Amniox launching NEOXTM Grafts at the American College of Foot and Ankle Surgeons Annual Scientific Conference Amniox Medical, Inc., a leader in Amnion

Explaination: Explanation: Placenta and chorion compose the fetal-maternal junction. The former is a trophoblast derivative and the latter is a derivative of both trophoblast and extraembryonic mesoderm. The other extraembryonic tissues such as the amnion, the yolk sac and the allantois are derived from the inner cell mass. Among them, the yolk sac and the allantois are endodermal derivatives whereas the amnion is an ectodermal derivative ...

Free, official info about 2015 ICD-9-CM diagnosis code 762.8. Includes coding notes, detailed descriptions, index cross-references and ICD-10-CM conversion info.

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This stock medical exhibit features normal fetal membrane anatomy during pregnancy. It reveals a sagittal cut-away view of a full term pregnant abdomen with fetus shown in-utero. A blue colored membranous capsule is shown surrounding the fetus. Additionally, a detailed section through the membrane identifies and labels the amnion, chorion, uterus and decidua.

This stock medical exhibit features normal fetal membrane anatomy during pregnancy. It reveals a sagittal cut-away view of a full term pregnant abdomen with fetus shown in-utero. A blue colored membranous capsule is shown surrounding the fetus. Additionally, a detailed section through the membrane identifies and labels the amnion, chorion, uterus and decidua.

Amniotic fluid, known as the patron saint of the baby, can play a role in alleviating external shocks, stabilizing the temperature in the uterus, inhibiting bacteria and reducing infection, lubricating after breaking water and disinfection of the birth canal. In fact, there is a word amniotic fluid in medicine, which is translated into Chinese as amniotic fluid for short. The origin of amniotic fluid has the meaning of nourishing and Yang, but its implication has the meaning of nurturing, sunshine and growth, but in fact, the most appropriate and scientific explanation is the amniotic fluid. < p > < p > because the fetus is wrapped in the amniotic membrane and grows and develops through the nutrient fluid in the amnion. Therefore, the clear amniotic fluid also represents that the baby can absorb more and better nutrition. < / P > < p > although I always want to have a natural birth, for the sake of the health and safety of the baby, a cesarean section was carried out in advance one ...

A human heteroploid epithelial-like cell line, designated as C-SPT, was recovered from one of several lots of human WI-38 fibroblast-like diploid cells that lacked evidence of Regan isozyme in early passages. In later passages with the growth of C-SPT cells, high activity of Regan type of alkaline phosphatase appeared as judged by l-phenylalanine inhibition, heat stability, and immunoelectrophoresis. The C-SPT cells appeared to be the progeny of an unidentified source contaminant rather than of transformed WI-38 cells. Comparative enzymological and immunological studies with HeLa and WISH cells confirmed the presence of Regan type of alkaline phosphatase as a characteristic of HeLa and established its presence in WISH cells. However, the specific activity of Regan isozyme in WISH cells was only 3 to 6% of that in C-SPT cells. The HeLa cells exhibited 33% of C-SPT level on the initial passage level studied, which after further cell passage declined to 8%. A HeLa clone was isolated showing very ...

My doc excised two pterygium from the same eye (one located toward the inner part nasal and one located toward the outer part tempor

Dr Ming Wang is a world-know eye surgeon with specialties in 3D bladeless all-laser LASIK and laser cataract. Dr wang is also a philanthropist and community leader.

The mechanisms responsible for the onset of labor in women are not completely understood. Growing evidence suggests that bacterial products such as endotoxin could stimulate monocytes or macrophages to produce inflammatory cytokines such as IL-1, TNF, IL-6 and IL-8, which in turn stimulate prostaglandin biosynthesis by amnion, chorion and decidua and result in the onset of labor. Amniotic fluid IL-6 could be a sensitive indicator for prospective diagnosis of acute histologic chorioamnionitis, so amniotic fluid IL-6 is considered as an important cytokine. IL-13 and TIMP-3, recently described, have been known to relate to IL-6. IL-13, a novel T-helper cell lymphokin, has been shown to upregulate IL-6 production in keratinocytes, endothelial cells and mesothelial cells, whereas it downregulates IL-6 production in human monocytes. Tissue inhibitor of metalloproteinase-3(TIMP-3), new type distinct from TIMP-1 and TIMP-2, is known to be stimulated by IL-6. In this study , we examined C3H pregnant mice ...

A typical human childbirth will begin with the onset of contractions of the uterus. The frequency and duration of these contractions varies with the individual. The onset of labour may be sudden or gradual. A gradual onset with slow cervical change towards 3 cm (just over 1 inch) dilation is referred to as the latent phase. A woman is said to be in active labour when contractions have become regular in frequency (3-4 in 10 minutes) and about 60 seconds in duration. The now powerful contractions are accompanied by cervical effacement and dilation greater than 3cm. The labour may begin with a rupture of the amniotic sac, the paired amnion and chorion (breaking of the water). The contractions will accelerate in frequency and strengthen. In the transition phase from 8cm-10cm (3 or 4 inches) of dilation, the contractions often come every two minutes are typically lasting 70 - 90 seconds. Transition is often regarded as the most challenging and intense for the mother. Some mothers say things ...

FIG 11.1 Incision line opening. (A) Incision line opening occurring after implant placement. (B) Post allograft incision line opening. (C) Membrane graft dehiscence. (D) Block graft with fixation screw exposure. The prevalence of ILO has been shown in studies to range from 4.6% to 40% around submerged implants.1,2 In a study by Mendoza, 37% had no…

Fetus of a cow, with its membrane. Labels: a, placenta; b, chorion with the allantois adherent to its inner surface; c, amnion seen through outer covering; d, fetus seen through its covering.

Use this economical female pelvis model on its own or with Fetal Model, Perineal Cloth Model, and Placenta/Cord/Amnion/Chorion Model to help expectant parents understand the process of labor and birth.

A human embryo of the fourth week. I, the chorion; 3, part of the amnion; 4, umbilical vesicle with its long pedicle passing into the abdomen; 7, the heart; 8, the liver; 9, the visceral arch destined to form the lower jaw, beneath which are two other visceral arches separated by the branchial clefts; 10, rudiment of the upper extremity, 11, that of the lower extremity; 12, the umbilical cord; 15, the eye; 16, the ear; 17, cerebral hemispheres; 18, optic lobes, corpora quadrigemina.. ...

Anatomical structure in a pouch. Gestational sac: membranes enveloping the foetus or embryo. Composed by the fusion of chorion and amnion.

Anatomical structure in a pouch. Gestational sac: membranes enveloping the foetus or embryo. Composed by the fusion of chorion and amnion.

This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.