Revert Destaining Solution is for use with the Revert 700 Total Protein Stain. It consists of 0.1 M sodium hydroxide, 30% (v/v) methanol, in water. This product requires additional fees for hazardous shipping costs.. This product is sufficient for 20 mini Western blots (10 mL/blot).. ...

O pinhão é a semente comestível da Araucaria angustifolia, sendo consumido no sul e sudeste do Brasil principalmente na sua forma in natura. A falta de diversidade em relação ao seu consumo acarreta a sobra da safra e a sua utilização como ração animal, o que diminui o valor de comercialização do produto, prejudicando os ganhos econômicos do produto. A semente do pinhão é composta de 34% de amido e um conteúdo muito baixo de proteínas e lipídios, o que a torna favorável para a obtenção de amido, aditivo amplamente utilizado pela indústria de alimentos. O objetivo deste trabalho foi caracterizar uma nova fonte de amido a partir de semente de Araucária angustifolia, através de um estudo detalhado da metodologia de extração do amido de pinhão, das características físico-químicas, funcionais e reológicas do grânulo e de sua modificação estrutural. Primeiramente foi determinado um protocolo para a extração do amido e realizado o estudo do efeito do tratamento ...

A cause of bad breath is protein stains on your teeth. Using Propolinse mouthwash, you can see the protein stains wash away in your mouth thanks to a special formula. Your mouth will feel refreshed and clean after use. The mouthwash protects against germs that cause bad breath. How to Use: Gargle half a cap-full for 20

Fig. 2C -STAT 3 loading control is actually the b-acting loading control from Fig. 4B of 20103603. (3 years apart, and hes using the same image for 2 totally different things. Also, theres evidence of material being erased from the top-right corner of the image on both cases - thats how we know they are the same!) ...

Fig. 2C -STAT 3 loading control is actually the b-acting loading control from Fig. 4B of 20103603. (3 years apart, and hes using the same image for 2 totally different things. Also, theres evidence of material being erased from the top-right corner of the image on both cases - thats how we know they are the same!) ...

5 Degree Spot (Optional) The Sekonic 5 Degree Spot Viewfinder for the LITEMASTER PRO L-478D Series of light meters is an attachment spot viewfinder that features a 5° metering area for more precise, reflected-light measurment.Built In PocketWizard PocketWizard triggering and Flash Power Setting w/ControlTL radios. Control Nikon, Canon and select studio flashes with a touch of a fingerFull Featured Meter For CinematographersDial in your exact shutter angle and frame rate to get exact exposures, and evaluate the lighting in your scene.Full Featured Meter For CinematographersDial in your exact shutter angle and frame rate to get exact exposures, and evaluate the lighting in your scene.Whats in the Box• Sekonic L-478DR-U LITEMASTER PRO for PocketWizard System Light Meter• Anti-Glare Sheet for LCD Screen• Lens Cap• Strap• Soft Case• Limited 1-Year Warranty

In detail, we demonstrated the capacity of the combined approach to target the CSCs compartment in mCRPC models and unveiled a novel molecular mechanism underlying this synergism based on the inhibition of the oncogene YAP activity. in 22Rv1 in cell adhesion condition, in 1st generation spheres and in 2nd generation spheres. -actin was used as housekeeping control gene to normalize RT-PCR reactions. C. Basal expression of NANOg evaluated by western blotting in 22Rv1 cells in cell adhesion condition, in 1st generation spheres and in 2nd generation spheres. tubulin was used as loading control. D. Surface marker expression (CD44 and CD113) was determined by circulation cytometry on 22Rv1cells at basal level in 22Rv1 in both cell adhesion condition and 1st generation spheres. E. CTGF mRNA expression evaluated by RT-PCR at basal level in Noscapine 22Rv1 in both cell adhesion condition and in 1st generation spheres. -actin was used as housekeeping control gene to normalize RT-PCR reactions ...

Rockland Immunochemicals specializing in Akt Signaling Antibodies Antibody, Custom Assay Development, Custom Antibody Production, Loading Control Antibodies, DyLight Dye Conjugated Antibodies, Secondary Antibody Conjugates, Custom Monoclonal Antibody Production, Anti-GFP Green Fluorescent Protein, Multiplex Fluorescent Western Blotting Assay, Akt PI3 Kinase Signaling Antibodies, Western Blot Blocking Buffer, Streptavidin Peroxidase HRP ELISA, DyLight Dye Immuno fluorescence microscopy, NFkB p65 Antibody, High Content Screen Assay. Rockland Immunochemicals has supported the life science industry for over 40 years with a full range of the highest quality primary and secondary antibodies, fusion proteins, substrates, standards, and controls for basic research, assay development, and preclinical studies.

a) pRB was induced by tetracycline in a stably transfected SAOS2 cell line (SAOS-tetRB) and protein samples for immunoblotting were harvested at various times after pRB induction. GAPDH, loading control. Uncropped images of the gels are shown in the Supplementary Information, Fig. S6e. (b) pRB-mediated G1 arrest of corresponding samples was monitored by FACS counting BrdU-incorporating S-phase cells after tetracycline treatment of parental SAOS2 cells that do not carry inducible pRB (parental, grey bars), and of pRB-inducible SAOS2 as in a (tet-pRB). (c) pRB-APCcdh1 association was monitored over time by immunoprecipitation using anti-pRB antibodies. The pRB antibody concentration was limiting with regard to extract to obtain equal quantities of immunoprecipitated pRB at the different times. Ig: heavy chain. Uncropped images of the gels are shown in the Supplementary Information, Fig. S6f. (d, e) As controls for the specificity of APCcdh1- and pRB-mediated Skp2 protein turnover, SAOS-tetRB cells ...

Figure 7. siRNA-mediated depletion of 4E-T affects mRNA turnover. HeLa cells were transfected with siRNA against 4E-T or a control (Ctrl) siRNA (4AIII inverted). Protein and RNA were harvested for use in Western (C and D) and Northern (A, B, and E) analysis, respectively. (A and B) HeLa cells were transfected with siRNA against 4E-T or with control siRNA and later cotransfected with pTet-β-globin or pTet-β-globin c-fos or GM-CSF ARE and pTet Off. Cells were treated with doxycycline (1 μg/ml) ∼72 h after transfection to block transcription. Total RNA was isolated at 0.5, 2, 3, and 4 h for β-globin/GM-CSF ARE assays (A) and 0.5, 1.5, 3, and 6 h for β-globin/c-fos ARE assays (B) and analyzed by Northern blot. GAPDH served as a loading control. mRNA half-lives were calculated from Northern blots and normalized against GAPDH levels. (C) Protein extracts were collected at 6 h after treatment with doxycycline (1 μg/ml) from cells that were transfected with the reporter plasmid and pTetOff and ...

Fig. 6. WDR34 is required for ciliogenesis. Cells were transfected with siRNA duplexes to suppress WDR34. (A-C) Silencing of WDR34 (red) causes a defect in ciliogenesis (acetylated tubulin labelling, green). Boxed regions show enlargements. Note that in C, the lower inset illustrates that a robust loss of WDR34 correlates with a failure to form cilia. The upper box shows that those cells that are less effectively suppressed still retain the ability to form primary cilia and that these remaining cilia are longer than controls (compare with A). (D) Immunoblotting of WDR34-depleted cells and lamin-A/C-depleted control, α-tubulin is used as a loading control. (E) Quantification of the ciliogenesis defect on WDR34 suppression. *P,0.01 using ANOVA with Dunnetts post-hoc test. Error bars represent s.d. (F) As in C, remaining cilia in WDR34-depleted cells were significantly longer than in control cells. (G) The typical pericentrosomal accumulation of WDR34 seen in control cells (arrows) is lost on ...

Figure 1. Cdh1 phosphorylation at Cdk sites promotes Cdh1 stability. A, Schematic of nine conserved sites of potential Cdk phosphorylation in Cdh1 (S/T-P), numbered according to human Cdh1. B, Lysates of 293T cells transfected with GFP-Cdh1 WT, 9A, or 9D were immunoblotted using a polyclonal antibody to Cdh1 or Erk, the latter to serve as a loading control. Quantitation of Cdh1 levels normalized by Erk revealed that the levels of the 9D and 9A mutant proteins were, respectively, increased by 122% and reduced by 57% relative to wild-type Cdh1 in 293T cells (average of 4 experiments). C, Lysates of Neuro2A cells transfected with GFP-Cdh1 WT, 9A, or 9D and treated with MG132 (5 μm) or the vehicle control DMSO were immunoblotted for GFP or Erk. The levels of the 9D and 9A mutant proteins were, respectively, increased by 150% and reduced by 37% relative to wild-type Cdh1 in Neuro2A cells (average of 2 experiments). MG132 treatment, respectively, increased WT, 9A, and 9D levels by 80, 134, and 9%. D, ...

Fig. 3. Glucose is required for the PM localization of K-RasG12V and LactC2 in cells overexpressing ATP5G1(1-67). (A) Cell lysates of MDCK cells stably co-expressing mGFP-K-RasG12V or mGFP-H-RasG12V with RFP alone (−) or ATP5G1(1-67)-RFP (+) were immunoblotted for phospho-ERK, phospho-Akt (S473), and mGFP-RasG12V, and quantified (mean±s.e.m.) from three independent experiments. Significant differences between cells not expressing (−) or expressing (+) ATP5G1(1-67) were assessed using Students t-test (*P,0.05; ***P,0.001). Representative blots of are shown, with total ERK, total Akt and actin used as loading controls. (B) MDCK cells stably expressing mGFP-K-RasG12V in the presence (+) or absence (−) of ATP5G1(1-67)-RFP were maintained in complete growth medium (DMEM+10% FBS+2 mM L-glutamine). When cells reached full confluence, the growth medium was replaced with fresh complete growth medium and cells were further incubated for indicated time points. Cells were fixed with 4% PFA and ...

Images: MPZ and PMP22 expression in the sciatic nerve of WT and Nox4−/− mice after SNI. A, Western blot analysis of the myelin-specific proteins MPZ and PMP22 in the day 14 SNI sciatic nerve (proximal nerve stump) and the uninjured control sciatic nerve. GAPDH was used as loading control. Note that MPZ and PMP22 protein expression is significantly decreased after SNI in WT mice but not in Nox4−/− mice. n = 3 mice per group. Data are presented as mean ± SEM (*p , 0.05). B, Immunostaining of the day 14 SNI sciatic nerve shows increased MPZ immunoreactivity in Nox4−/− mice compared with WT mice, whereas immunoreactivity of the neuronal marker NF200 is similar in both genotypes. Scale bar, 10 μm. ...

Fig. 4B - this is a b-actin blot. It is IDENTICAL to a STAT3 loading control used in Figure 2C of PMID 17505005 (Clin Cancer Res. 2007 May 15;13(10):3024-32.). Something is erased from the top right coner in both images, so we know theyre the same. ...

Figure and Images: Expression of functional MMP-9 by GLV-1h255-infected tumor cells. (A) Expression cassettes of GLV-1h68 and GLV-1h255. In GLV-1h255 the insert in the Tk locus was replaced by the human mmp-9 gene under control of the PSE promoter. PSEL, synthetic early/late promoter; PSE, synthetic early promoter; P7.5, VACV p7.5 K early/late promoter; P11, VACV p11 late promoter; Tk, thymidine kinase locus, Ha, hemagglutinin locus. (B) Expression of virus-encoded MMP-9 (92 kDa) in GLV-1h255 infected PC-3 cells and supernatants in vitro, β-actin (42 kDa) was used as a loading control. (C) Activity of the MMP-9 protein was tested by gelatin zymography. Lysates and supernatants of infected A549 cells were isolated and separated by non-reducing SDS-PAGE. In zymography, cleavage of the substrate by MMP-9 resulted in a clear band. ...

Neutral cobalt(II) complexes of the dianionic tetrapodal pentadentate ligand B2Pz4Py, in which borate linkers supply the anionic charges, are reported. Both the six-coordinate THF adduct 1-THF and the five-coordinate THF-free complex 1 are in a high-spin S = 3/2 configuration in the ground state and have been structurally characterized by X-ray crystallography. These two Co(II) starting materials react rapidly with aryl azides of moderate steric bulk. The thermodynamic products of these reactions are low-spin, diamagnetic, Co(III) amido complexes that are either monomeric, when an external hydrogen atom source such as 1,4-cyclohexadiene is present, or dimeric products formed via C-C coupling of the azide aryl group and internal transfer of H• to the nitrogen. These products are fully characterized and are rare examples of octahedral Co amido compounds; structural determinations reveal significant pyramidalization of the amido nitrogens due to π-π repulsion wherein the amido ligand is ...

N-[2-(Substituted amido)phenyl]-N,N-bis-carbonylguanidines bearing an optionally substituted phenoxy, phenylthio, phenylsulfinyl or phenylsulfonyl group in the 4- or 5-position of the 2-(substituted amido)phenyl group are anthelmintic agents. The compounds, of which N-(2-acetamido-4-phenylthiophenyl)-N,N-bis-carbomethoxyguanidine is a typical example, are prepared through the reaction of isothiourea-S-alkyl ether and an appropriately substituted 2-aminoanilide.

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4HVG: 3-Amido Pyrrolopyrazine JAK Kinase Inhibitors: Development of a JAK3 vs JAK1 Selective Inhibitor and Evaluation in Cellular and in Vivo Models.

EZBlue Coomassie Brilliant Blue G-250 colloidal protein stain improves protein electrophoresis results while significantly reducing staining time. Conveniently packaged, EZBlue requires no messy weigh-ups or additions of methanol or acid. As a colloidal stain, it reacts only with proteins, not the gel itself.

A quick glance past the purulent picture reveals such gems as guitar-string needles, computer ink-jet printer cartridge, and park bench. Interesting also that the pustules were peripheral to the ink itself, perhaps indicative of lymphatic drainage of the tattoo site.. Taras comment thread was abuzz when she revealed herself as having not one, but two, tattoos. Speculation continues to abound as to the physical sites of her body ink, owing to her own statement, Theyre not in oft-seen areas under normal attire, but neither are they anywhere naughty.. This led me, of course, to wonder how exactly do tattoos work. Like anyone who watches CSI knows, the epidermis is constantly sloughing off dead skin cells as new skin rises to replaces it. This is why Coomassie Blue protein stain colors your fingers for a couple of days, until those skin cells fall off.. Being tattoo-naive, I was aware that needles are involved in the process, but I hadnt truly appreciated that the dermis underlying the ...

A range of chemical techniques were utilised for the enhancement of footwear impressions deposited on a variety of fabric types of different colours with urine as a contaminant. A semi-automated stamping device was used to deliver test impressions at a set force to minimise the variability between impressions; multiple impressions were produced and enhanced by each reagent to determine the repeatability of the enhancement. Urine samples from different donors were analysed using a spectrofluorophotometer revealing differences between individuals. Results indicated that the enhancement of footwear impressions in urine was possible using amino acid staining techniques whereas protein stains failed to achieve successful enhancement.. ...

Multi-purpose oxidizing cleaner for organic soils and molds Developed by AiRD Chemistry in consultation with the Australian wine industry, Destainex products are proprietary sodium percarbonate based cleaning agents with sanitizing abilities. These highly effective formulations can be used at low levels to remove wine color, protein stains, mold, mildew, and biofilms from wine contact surfaces such as: stainless

Multi-purpose oxidizing cleaner for organic soils and molds Developed by AiRD Chemistry in consultation with the Australian wine industry, Destainex products are proprietary sodium percarbonate based cleaning agents with sanitizing abilities. These highly effective formulations can be used at low levels to remove wine color, protein stains, mold, mildew, and biofilms from wine contact surfaces such as: stainless

ABSTRACT: To address questions regarding the potential impact of elevated total phosphorus (TP) inputs (due to relaxed regulations of TP loading), a series of TP enrichment experiments were conducted at pelagic stations in the 3 hydrologically distinct basins of Lake Erie. Results of nutrient assimilation measurements and assays for nutrient bioavailability suggest that the chemical speciation, and not concentration, of nitrogenous compounds may influence phytoplankton community structure; this in turn may lead to the selective proliferation of cyanobacteria in the eastern basin of the lake. Assays with cyanobacterial bioluminescent reporter systems for P and N availability as well as Ntot:Ptot assimilation ratios from on-deck incubation experiments support this work. Considered in the context of a microbial food web relative to a grazing food web, the results imply that alterations in current TP loading controls may lead to alterations in the phytoplankton community structure in the different ...

Fig. 4 Enhanced phosphorylation of BLT1 by sequential exposure to increasing concentrations of LTB4.. (A) Intracellular [Ca2+] in HeLa cells expressing HA-BLT1/0N that were pretreated with 10 nM LTB4 or vehicle before being stimulated with 100 nM LTB4. The response to adenosine triphosphate (ATP) was included as a positive control. (B) HeLa cells expressing HA-BLT1/0N were stimulated with LTB4 as indicated. Membrane fractions were subjected to Phos-tag SDS-PAGE and immunoblotting for HA. WCLs were subjected to SDS-PAGE and immunoblotting for ERK1/2 and phosphorylated ERK1/2 (p-ERK1/2). β-Actin is an experimental and loading control. (C) RBL-2H3, CHO-K1, and Ba/F3 cells expressing HA-BLT1/0N were stimulated with LTB4 as indicated. Membrane fractions were separated by Phos-tag SDS-PAGE and immunoblotted for HA. (D) HeLa cells expressing HA-BLT1/0N were stimulated with LTB4 in the presence or absence of 100 nM CP105696 (CP) or ZK158252 (ZK). Membrane fractions were separated by Phos-tag SDS-PAGE ...

Figure 1. Inhibition of CDK1 enhances cell death induction by cobimetinib in BRAF-mutant colorectal cancer cells. A, A panel of BRAF mutant colorectal cancer cells and A375 melanoma cells were treated with cobimetinib (Cobi) or vemurafenib for 48 hours and expression of pERK1/2 and apoptosis-related proteins were determined by immunoblotting. Basal levels of CDK1 expression were examined in colorectal cancer cell lines with mutant or wild-type BRAF or KRAS genes. B, RKO and HT29 cells were transiently transfected with siRNA against CDK1 versus nontargeting control siRNA. After treatment with cobimetinib for 24 or 48 hours, immunoblotting using designated antibodies or Annexin V labeling was performed, respectively; *, P , 0.05. In HT29 and WiDr colorectal cancer cells with siRNA knockdown of ERK1/2, expression of CDK1, pERK1/2 and ERK1/2 was detected by immunoblotting. Tubulin was probed as a loading control. C, RKO and HT29 cell lines were treated with cobimetinib, R0-3306 (CDK1 inhibitor), or ...

FIG. 7. Reversibility of Ni-induced gene expression. Expression of target genes in strain CC125 at 3 × 106 cells/ml is shown in lane 1 (t = 0). The culture was treated with either 25 μM NiCl2 for 5 h (lane 2) or 50 μM EDTA for 5 or 19 h (lanes 7 and 8) The reversibility of the 5 h Ni2+ treatment was ascertained either by addition of excess EDTA (50 μM) to chelate the Ni2+ ions or by transfer to fresh medium and then sampled 5 or 19 h later to assess gene expression (lanes 5 and 6 and lanes 9 and 10, respectively). Ni-treated cultures were maintained in parallel for another 5 or 19 h as a control for the EDTA treatment (lanes 3 and 4, respectively), or in the case of the washed cells, NiCl2 was added back, and the culture sampled 5 or 19 h later (lanes 11 and 12). The total time of exposure to Ni2+ was, therefore, 10 or 24 h. Total RNA was isolated and analyzed by RNA blot hybridization for Cyc6 and Cpx1 expression. Cβlp expression was used as a loading control. The circular arrow indicates ...

FIG 2 Production of FlaEF and their roles in motility and flagellation of V. vulnificus. (A and B) FlaE and FlaF. Cell lysates and the culture supernatants were prepared from the wild-type, ΔflaABCD, and ΔflaJ strains of V. vulnificus. Sixty micrograms of crude cell extracts (A) and 2 μg of the concentrated proteins in culture supernatants (B) were subjected to SDS-PAGE and then Western blot analysis using polyclonal antibodies reacting with all the flagellin-like proteins from FlaA to FlaF. As loading controls for cell lysate and culture supernatant, the antibodies specific to IIAGlc and OmpU were utilized, respectively. The ΔflaJ strain was included as a negative control in this assay. Each immunoreactive band detected by specific antibodies is indicated with an arrow. Numbers on the left sides of the blots are molecular weight markers, in kilodaltons. (C) Motility on a soft agar plate. Fresh cultures of ΔflaABCD, flaE::nptI, ΔflaF, and ΔflaEF strains were spotted onto a soft agar ...

Fig. 2A,B,C and 4B - same b-actin loading control used for dose response and time course experiments. First 10 lanes of b-actin in 4B are the same as all 10 lanes in 2B. ...

Fig. 1. HRC is upregulated in fibrotic liver. (A) Representative immunohistochemical staining shows the expression of HRC in human samples. S0 represents no inflammation and no fibrosis of liver, S1 represents inflammation in portal area and peri-sinusoid fibrosis, S2 represents mild inflammation and fibrous septum formation. Scale bars: 100 µm. (B) Representative H&E, Masson and Sirius Red staining (100×) of TAA-induced liver fibrosis in rats. W, weeks. 4W represents rats treated with TAA for 4 weeks, 8W represents rats treated with TAA for 8 weeks. (C) Box plot (25th percentile, mean, 75th percentile) of the mRNA levels of α-SMA and HRC in TAA-induced liver fibrosis in rats measured by RT-qPCR (n=6). *P,0.05, **P,0.01. (D) Representative image shows the protein levels of α-SMA and HRC in TAA-induced liver fibrosis in rats. GAPDH was used as a loading control. ...

Squaric Acid Ester Amido Polyethylene Glycols are a novel class of reactive and chemoselective PEGylation reagents. The squaric acid PEG´s are chemoselective towards the amino groups of Lysin in protein PEGylation whereas the guanidine function of Arginine, the hydroxyl group of Serine and the imidazole function of Histidine are not affected. Literature: L 59 ...

Complex (I) was synthesized in the course of our studies on amido complexes of zirconium (Kempe, 2000). The compound was obtained in the reaction Zr(NEt)4 with iPr2C6H3NH2 (molar ratio 1:2). Complex (I) contains four amido ligands (two NEt2 and two iPr2C6H3NH) and one amino ligand (HNEt2). Analyzing bond lengths in title compound it is easily spotted that bond Zr-N3 is much longer than other bonds. It is caused by the fact, that ligand containing N1 is amine ligand. Difference between length of bonds Zr-N between NEt2 and HNEt2 is about 0.25 Å. Distances between N atoms of iPr2C6H3NH and Zr are both about 2.12 Å and very similar to related Zr(IV) amido complexes (Profilet et al., 1990; Blake et al., 1997). In case of two NEt2 ligands distances Zr-N differ by about 0.12 Å but both are in the range typical for zirconium complexes with diethylamido ligands (Porter et al., 2004; Ghesner et al., 2006). Comparing angles between N1-Zr1-N2, N2-Zr1-N5 and N5-Zr1-N1 [table 1] it can be seen that they ...

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Provided is a process for diastereoselectively preparing compounds of the formula ##STR1## which includes the step of subjecting a compound of the formula ##STR2## to a salt whose anion is a nucleophilic base whose conjugate acid has a pKa in the range of between about -7 to about 14, or a silylated derivative of the salt; wherein R.sub.1 is said nucleophile; R is hydrogen, or protected amino, R.sub.2 is R.sub.4 as defined herein below; R.sub.3 is a leaving group; and R.sub.4 is hydrogen, C.sub.1 -C.sub.6 alkyl, or a group of the formula wherein R.sub.6 is 2-furyl, naphthyl, phenyl, phenyl substituted with 1, 2 or 3 substituents selected from C.sub.1 -C.sub.6 alkyl, C.sub.1 -C.sub.6 alkoxy, C.sub.1 -C.sub.6 alkylthio, nitro, halo, carboxy and amido; or R.sub.6 is a group of the formula in which R.sub.7 is selected from C.sub.1 -C.sub.6 alkyl, C.sub.2 -C.sub.6 alkenyl, benzyl, phenyl, or benzyl or phenyl substituted with 1, 2 or 3 substituents selected from C.sub.1 -C.sub.6 alkyl, C.sub.1 ...

Dear Histonetters, I am looking for stains that will identify the basic contents of cultured, mammary alveoli (mammospheres) of Kangaroo. Broad lipid ( ANY FAT STAIN THAT CAN BE DONE ON PARAFFIN EMBEDDED TISSUE???POSSIBLE??), carbohydrate (PAS?? ANY OTHER SUGGESTIONS) and protein stains (???) are required to determine what - IF any, different hormone combinations have/ effect on milk production. Any suggestions would be wonderful......our Histologist has made several good suggestions for me to try & we are both interested to see WHAT POSSIBILITIES/suggestions the scientific minds in Histo-land come up with. Thankyou in advance & Cheers, Sonia -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT.of ZOOLOGY EMAIL: [email protected] THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONES WATCHING Q: Whats a specimen? A: An Italian astronaut. ...

Electroblotted proteins noncovalently bound to polyvinylidene difluoride (PVDF) membranes are typically sequenced using adsorptive sequencer protocols (gas-phase or pulsed-liquid) that do not require a covalent linkage between protein and surface. We have developed simple chemical protocols where proteins are first electroblotted onto unmodified PVDF membranes, visualized with common protein stains, and then immobilized for solid-phase sequence analysis. Adsorbed, stained proteins are first treated with phenylisothiocyanate (PITC) to modify alpha and epsilon amines. The protein is then overlayed with a solution of 1,4-phenylene di-isothiocyanate (DITC), followed by a few microliters of a basic solution containing a poly(alkylamine). As the polymer dries onto the surface both polymer and remaining protein amino groups are crosslinked by DITC. The protein is thus immobilized to the membrane surface by entrapment in a thin polymer coating. The coating is transparent to the degradation chemistry, ...

a, smFISH showing the ectopically expressed CCAT1-5L lncRNA and MYC promoter RNA are colocalized. CCAT1-5L, red; MYC, green; DAPI, blue. b, Dual RNA-DNA FISH showing the ectopically expressed CCAT1-5L RNA is colocalized with MYC locus. CCAT1-5L lncRNA, red; MYC locus, green; DAPI, blue. c, Western blot showing the ectopically expressed CCAT1-5L boosts MYC levels in HeLa cells. GAPDH served as a loading control. The MYC level is quantified in the middle. d, The depletion or ectopic expression of CCAT1-5L (5L) in HeLa cells influences the proliferation rate. e, Knockdown or ectopic expression of CCAT1-5L affects colony formation. f, CCAT1-5L is critical for cell metastasis in a transwell assay. Scale bar, 50 μm. The experiments in a-c were independently repeated three times with similar results. Data in d-f are mean ± s.d.; n = 3 biological replicates, two-tailed, unpaired t-test. ...

Fig. 6 Oscillatory localization of TMEM24 at the PM affects insulin secretion in insulinoma cells.. (A) (Top) High-glucose stimulation of WT INS1 cells triggers oscillations of TMEM24 (TMEM24-mCherry, top) and calcium (GcaMP6s, bottom) opposite in phase as monitored by TIRF microscopy. (Inset) Depletion of TMEM24 (peak inverted) reaches maximum after the peak of calcium influx. Dotted lines indicate corresponding peaks. (Bottom) Kymograph shows that TMEM24 and calcium oscillations are opposite in phase after glucose stimulation. (B) WT and TMEM24 KO INS1 cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies against TMEM24 and insulin, showing no difference in insulin biogenesis in the KO cells. Top band in anti-insulin panel is proinsulin, whereas bottom band is insulin. GAPDH was used as a loading control. (C) Calcium oscillations (monitored by GcaMP6s) are abrogated after glucose stimulation in TMEM24 KO INS1 cells. (D) Exogenous overexpression of TMEM24-mCherry in KO cells ...

Figure 1. Intratumoral administration of a STAT3 decoy oligonucleotide abrogates target gene expression in patients with HNSCC. A, schema of phase 0 trial. Biopsies of HNSCC tumors were performed and the tumors were injected with a single dose of a STAT3 decoy oligonucleotide (or saline) followed by tumor resection and analysis of target gene expression in the paired tumor samples. OR, operating room. B, downmodulation of STAT3 target genes in representative HNSCC tumors injected with STAT3 decoy as shown by Western blot analyses. Whole-tumor lysates were prepared from 4 HNSCC tumors before and after injection with the STAT3 decoy enrolled on the first dose tier. Proteins (40 μg) were resolved on a 12.5% SDS-PAGE gel and subjected to immunoblotting with anti-Bcl-XL and cyclin D1 antibody. β-Actin was used as a loading control. C, representative images of immunohistochemical staining for cyclin D1 protein expression before and after injection of STAT3 decoy oligonucleotide or saline from 6 ...

FIG. 3. RPA for ctxAB transcript in various vieSAB mutant strain backgrounds. (A) Genetic organization of the V. cholerae ctxAB operon. Putative promoters are indicated by arrows. Probes designed to detect the ctxA and ctxB portions of the message are indicated by hatched bars drawn to scale. (B) RPA for ctxA message. Total RNA (1 μg) isolated from strains grown under the AKI inducing condition for 7 h as described in Materials and Methods was analyzed by using the ctxA-specific probe. A probe against rpoB was included as an internal loading control. Lanes: 1, RNA marker; 2 and 3, undigested ctxA and rpoB probes, respectively; 4 and 5, AC-V66 RNA ctxA probe only and rpoB probe only, respectively. Lanes 6 to 12 all contain both ctxA and rpoB probes. Lanes: 6, AC-V66; 7, Bah-2; 8, AC-V494; 9, AC-V752; 10, AC-V765; 11, AC-V279; 12, AC-V323b. wt, wild type. Protected bands of the expected sizes are indicated by arrows to the right. The sizes of the molecular weight markers in base pairs are given ...

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Squaric Acid Ester Amido Polyethylene Glycols are a novel class of reactive and chemoselective PEGylation reagents. The squaric acid PEG´s are chemoselective towards the amino groups of Lysin in protein PEGylation whereas the guanidine function of Arginine, the hydroxyl group of Serine and the imidazole function of Histidine are not affected. Literature: L 59 ...

Next-generation sequencing has generated a need for a broadly applicable method to remove unwanted high-abundance species prior to sequencing. We introduce DASH (Depletion of Abundant Sequences by Hybridization). Sequencing libraries are DASHed with recombinant Cas9 protein complexed with a library of guide RNAs targeting unwanted species for cleavage, thus preventing them from consuming sequencing space. We demonstrate a more than 99 % reduction of mitochondrial rRNA in HeLa cells, and enrichment of pathogen sequences in patient samples. We also demonstrate an application of DASH in cancer. This simple method can be adapted for any sample type and increases sequencing yield without additional cost.

For critical research, you need reliable protein detection, and Life Technologies provides a complete suite of labeling tools for any level of target abundance. Our fluorescent products portfolio is the most peer-referenced in life science research. Check out this simple guide and see which tool is right for your protein. • High-abundance target-primary conjugate, no amplification needed • Medium- to high-abundance target-secondary conjugate, modest amplification • Medium- to low-abundance target-streptavidin conjugate, elevate the signal • Low-abundance target-enzyme amplification, complex process for maximum signal enhancement

Vorrei descrivere nel dettaglio le proprieta di questo riso particolarmente adatto per risotti grazie alla ricchezza di amido che aiuta al momento della mantecatura ottima anche la tenuta di cottura che permette di mantenere il risotto in stand by per parecchi minuti in piu rispetto ad un normale riso commerciale, qualita importante dove i minuti fanno la differenza nelllambito di una collaborazione sala/cucina ...

CD1d-restricted natural killer T cells (NKT cells) possess a wide range of effector and regulatory activities that are related to their ability to secrete both T helper 1 (Th1) cell- and Th2 cell-type cytokines. We analyzed presentation of NKT cell activating α galactosylceramide (αGalCer) analogs that give predominantly Th2 cell-type cytokine responses to determine how ligand structure controls the outcome of NKT cell activation. Using a monoclonal antibody specific for αGalCer-CD1d complexes to visualize and quantitate glycolipid presentation, we found that Th2 cell-type cytokine-biasing ligands were characterized by rapid and direct loading of cell-surface CD1d proteins. Complexes formed by association of these Th2 cell-type cytokine-biasing αGalCer analogs with CD1d showed a distinctive exclusion from ganglioside-enriched, detergent-resistant plasma membrane microdomains of antigen-presenting cells. These findings help to explain how subtle alterations in glycolipid ligand structure can ...

TY - JOUR. T1 - Reducible poly(amido ethylenimine)s designed for triggered intracellular gene delivery. AU - Christensen, Lane V.. AU - Christensen, L.. AU - Chang, Chien-Wen. AU - Kim, Won Jong. AU - Kim, Sung Wan. AU - Zhong, Zhiyuan. AU - Lin, C.. AU - Engbersen, Johannes F.J.. AU - Feijen, Jan. PY - 2006. Y1 - 2006. N2 - Poly(amido ethylenimine) polymers, a new type of peptidomimetic polymer, containing multiple disulfide bonds (SS-PAEIs) designed to degrade after delivery of plasmid DNA (pDNA) into the cell were synthesized and investigated as new carriers for triggered intracellular gene delivery. More specifically, three SS-PAEIs were synthesized from Michael addition reactions between cystamine bisacrylamide (CBA) and three different ethylene amine monomers, i.e., ethylenediamine (EDA), diethylenetriamine (DETA), or triethylenetetramine (TETA). Complete addition reactions were confirmed by 1H NMR. The molecular weight, buffer capacity, and relative degree of branching for each SS-PAEI ...

FIG. 1. The SAHH gene is a Myc target gene. (A) P493-6 cells were incubated with doxycycline (Dox) to inhibit exogenous Myc expression or vehicle control (veh). Rat1A fibroblasts, primary MEFs, and IMECs were infected with retroviruses to express vector control, MycWT, or MycΔMBII, and pools of cells were drug selected. Primary murine T cells were incubated with IL-2, IL-7, and IL-15 for 24 h. Rat1A cells were incubated with a control siRNA (con si; cyclophilin B siRNA) or an siRNA directed against Myc (Myc si) for 24 h. Western blotting was performed to detect SAHH, Myc, β-tubulin, or POLR2A expression, as loading controls. (B) RNA was extracted from the cell lines indicated, and RT-PCR was performed to detect SAHH mRNA levels relative to GAPDH (or 18S rRNA for P493-6). Prim., primary. (C) In the IMEC lines indicated, SAHH mRNA was detected by RT-PCR (black bars) and m7G (cap-methylated) SAHH mRNA was detected by anti-m7G IP followed by RT-PCR (gray bars). (D) Rat fibroblasts expressing the ...

TY - JOUR. T1 - Evidence for Silica Surface Three- and Five-Membered Metallacycle Intermediates in the Catalytic Cycle of Hydroaminoalkylation of Olefins Using Single-Ti-Metal Catalysts. AU - Yaacoub, Layal F.. AU - Aljuhani, Maha A.. AU - Jedidi, Abdesslem. AU - Al-Harbi, Manal S.. AU - Almaksoud, Walid. AU - Wackerow, Wiebke. AU - Abou-Hamad, Edy. AU - Pelletier, Jeremie. AU - El Eter, Mohamad. AU - Cavallo, Luigi. AU - Basset, Jean-Marie. N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: The King Abdullah University of Science and Technology (KAUST) supported the research. The authors acknowledge core lab at KAUST for their assistance. L.C. and A.J. are grateful to the KAUST Supercomputing Laboratory for the resources. A.J. thank High-Performance Computing Center (AZIZ supercomputer) for the support.. PY - 2020/6/30. Y1 - 2020/6/30. N2 - The single-site silica-supported group IV metal amido complex [Ti(NMe2)4] gives the tris(amido)-supported fragment ...

Background The dynamic regulation of cell-cell adhesions is crucial for developmental processes, including tissue formation, differentiation and motility. Adherens junctions are important components...

Extract] the five-coordinate aluminium centre is distorted trigonal bipyramidal with the two aromatic nitrogens in axial positions and the two amido nitrogens and chloride atoms in the equatorial positions. The overall structure is similar to the related [AlCI(8-amidoquinoline)₂], [1] exept that in the present compound, the chelating ligand has a much smaller bite angle, forming a four-menmbered chelate ring rather than a five-membered ring.. ...