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Inflammatory mechanisms contribute to neurodegeneration in AD. Acute phase proteins such as the antichymotrypsin (a1ACT), pro-inflammatory cytokines IL-6 and IL-1, and activated microglial cells are all associated with neuritic plaques. a1ACT levels are elevated in AD, correlate with cognitive decline and serve as a biological marker of intervention. a1ACT is intimately associated with the 42-AA b peptide (Ab) in filamentous amyloid deposits and stimulates the polymerization of Ab into amyloid filaments. IL-6 is found in AD cortices prior to the onset of neuritic change, serum and stimulated PMNC IL-6 levels are higher in AD, and may induce b-amyloid protein deposition. Microglia, CNS resident inflammatory cells, produce IL-6 within the adult human brain, are IL-1a +, and are a prominent component of the neuritic plaque. Because the pathogenesis of AD appears in part immune mediated, we propose testing directly in humans whether ingested IFN-a can ameliorate AD. Because there is no good model ...

Macroscopically, the tumor was of yellow-greyish color and medium consistency. Histologic examination of both the primary and recurrent tumors showed solid nests of tumor cells surrounded by a small rim of connective tissue. The neoplasm consisted of polygonal cells with abundant, eosinophilic, granular cytoplasm and relatively sharp cellular margins. Cytoplasmic granules were distinctly PAS-positive and resistant to diastase digestion. There was remarkable cellular and nuclear polymorphism with hyperchromatic nuclei and prominent nucleoli (Fig. 2).There were small areas of foamy cells and perivascular lymphocytic aggregates. Mitoses were rarely registered. The tumor was moderately vascularized. Frank necrosis was not evident. Immunohistochemically, tumor cells were strongly positive for neuron-specific enolase, S-100 protein, a1-antitrypsin and a1-antichymotrypsin (Fig. 3), but negative for glial fibrillary acidic protein, vimentin, various cytokeratins, melanoma marker HMB 45, chromogranin A, ...

article{9714ffdf-1e21-475d-ac0b-daebdc2182ca, abstract = {Clearance of the amyloid-P peptide (A beta) as a remedy for Alzheimers disease (AD) is a major target in on-going clinical trials. In vitro studies confirmed that A beta is taken up by rodent astrocytes, but knowledge on human astrocyte-mediated A beta clearance is sparse. Therefore, by means of flow cytometry and confocal laser scanning microscopy (CLSM), we evaluated the binding and internalization of A beta 1-42 by primary human fetal astrocytes and adult astrocytes, isolated from nondemented subjects (n = 8) and AD subjects (n = 6). Furthermore, we analyzed whether alpha 1-antichymotrypsin (ACT), which is found in amyloid plaques and can influence A beta fibrillogenesis, affects the A beta uptake by human astrocytes. Upon over night exposure of astrocytes to FAM-labeled A beta 1-42 (10 mu M) preparations, (80.7 +/- 17.7)% fetal and (52.9 +/- 20.9)% adult A beta-positive astrocytes (P = 0.018) were observed. No significant difference ...

A A beta 42 abl acid phosphatase ACSM1 actin-general actin-alpha cardiac actin-alpha smooth muscle actin-muscle specific AE1/AE3 (cytokeratin) AF4 AGR2 AKT1 albumin alcian blue ALK alkaline phosphatase ALL1 alpha-1-antichymotrypsin alpha-1-antitrypsin alpha-fetoprotein alpha-lactalbumin AMACR AML1 androgen receptor APC API2-MALT1 Apolipoprotein D arginase1 ATM ...

The free/total PSA ratio (%fPSA) is an additional new parameter used in assessing PSA levels. Some authors 1,2 recommend that men with PSA levels of 4.1 to 10 ng/mL who are not suspected of having prostate cancer by whatever means should undergo ...

C-Peel Delivery Serum has multiple benefits. Formulated with Vitamin C, the serum acts as a skin protector against radiations, protecting skin from oxidative damage. Stimulates collagen and elastin synthesis. Moisturises the skin. Inhibits melanogenesis and eases epithelization. Hyaluronic acid exerts a natural effect

In this demonstration, students will observe a reaction between baking soda and vinegar in the presence of a variety of different heights of lit candles. The initial environment has plenty of oxygen present in order to sustain the candles flame; however the reaction will produce carbon dioxide which will cause the lit candles to extinguish in order of height. Students will analyze and compare the presence of the gases in the container and make determinations about the densities of each.. ...

In this lab, students will learn that even the smallest spaces can have their own climate. They will create a miniature sized terrarium and will observe how sunlight enters in order to warm the air, and in turn heat the soil and seeds. Condensation will build up in the container and the students can observe the water cycle in action, as well as the growth of the seeds over time.. ...

Yashraj Biotechnology is an ISO 13485:2003 certified company. Since 1999, we are engaged in manufacturing of Native & Recombinant antigens. We serve our high quality purified antigens to the worldwide, leading diagnostic companies. We currently manufacture CRP • HBsAg-Ay & Ad • CA 15-3 • CA-125 • CA 72-4 • CA 19-9 • B2M • PSA & PSA-ACT • CEA • AFP • A1M • B2M •CYS-C • PR3 • MPO • D-Dimer • NGAL • PAPP-A • sTfR • Ferritin •KFLC • LFLC • RBP. HE-4 , rec-SLO. Mabs- PSA • CRP • CA 125-3 • HbA1c. ...

Rabbit polyclonal AACT antibody. Validated in WB, IHC, sELISA and tested in Human. Cited in 1 publication(s). Immunogen corresponding to full length native protein (purified).

Hello again - the hormone shot is doing its thing - no side effects at all - June 2018 blood work is - - PSA - <0.008 -free PSA - <0.01 - testo...

The authors acknowledge the following members of the Okayama Urological Cancer Collaborating Group; Toyoko Yamato, M.D., Ph.D. (Okayama City Hospital); Michinao Saegusa, M.D., Ph.D. (Hiroshima Citizens Hospital); Toshihiko Asahi, M.D., Ph.D. (Kagawa Prefectural Central Hospital); Bunzo Suyama, M.D., (Mitoyo General Hospital); Tadashi Oeda, M.D., Ph.D. (Onomichi Municipal Hospital); Takanori Murakami, M.D. (National Okayama Hospital); Yoshio Maki, M.D., Ph.D. (Konko Hospital); Teruaki Akaeda, M.D., Ph.D. (Tsuyama Central Hospital); Masatoshi Noda, M.D., Ph.D. (Saint Maria Hospital); Teruhisa Ohashi, M.D., Ph.D. (Okayama Redcross Hospital); and Masahiro Hara, M.D., Ph.D. (Fukuyama Municipal Hospital). ...

Stenman UH, Leinonen J, Alfthan H, Rannikko S, Tuhkanen K, Alfthan O. A complex between prostate-specific antigen and α1-antichymotrypsin is the major form of prostate-specific antigen in serum of patients with prostatic cancer: assay of the complex improves clinical sensitivity for cancer. Cancer Res 1991; 51: 222-6 ...

Since 1987 her research has focused on the serpin family of SERine PRoteinase INhibitors, which play important regulatory roles in vivo. They now recognise that the physicochemical properties of the serpin structure that confer protease inhibitor activity also render these proteins sensitive to post-translational modifications (i.e. oxidation, nitrosylation, inter- or intramolecular interactions and non-specific cleavage). These altered forms of inhibitory serpins are detected in tissues and fluids recovered from inflammatory sites, but the important questions of how they are generated, what their biological activities are, and which of them are directly linked to pathologies and/or may be useful markers for characterisation of disease states, remain to be answered. Her objective is to address this challenge, focusing on the role of native (inhibitory) and post-translationally modified (non-inhibitory) forms of Alpha1-Antitrypsin in obstructive pulmonary disease and Alpha1-antichymotrypsin in ...

The superfamily of ser ine p roteinase in hibitors (serpins) is involved in numerous fundamental biological processes as inflammation, blood coagulation and apoptosis. Our interest is focused on the SERPINA3 sub-family. The major human plasma protease inhibitor, α1-antichymotrypsin, encoded by the SERPINA3 gene, is homologous to genes organized in clusters in several mammalian species. However, although there is a similar genic organization with a high degree of sequence conservation, the reactive-centre-loop domains, which are responsible for the protease specificity, show significant divergences. We provide additional information by analyzing the situation of SERPINA3 in the bovine genome. A cluster of eight genes and one pseudogene sharing a high degree of identity and the same structural organization was characterized. Bovine SERPINA3 genes were localized by radiation hybrid mapping on 21q24 and only spanned over 235 Kilobases. For all these genes, we propose a new nomenclature from SERPINA3-1 to

Serpins (serine protease inhibitors) comprise a superfamily of more than forty proteins which are implicated in various regulatory cascades in vivo such as blood coagulation, fibrinolysis, complement activation, matrix proteolysis, and inflammation. Since these 45 kD proteins undergo a massive beta-sheet rearrangement during the course of their biological function, serpins are intriguing from the perspectives of protein folding as well as medical relevance. Antichymotrypsin has been engineered to be an inhibitor of human neutrophil elastase in vitro, and the engineered serpin exhibits antiinflammatory properties in vivo. We have determined the structure of this serpin, and current work focuses on a structure-assisted approach toward engineering the specificity and stability of the active serpin. Additionally, we will determine the structure of the serpin in its complex with a target serine protease, and we will also determine the structures of other members of the serpin superfamily, e.g., those ...

TY - JOUR. T1 - Selection by phage display of a mustard chymotrypsin inhibitor toxic to pea aphid.. AU - Ceci, L.R.. AU - Volpicella, M.. AU - Conti, S.. AU - Gallerani, R.. AU - Beekwilder, M.J.. AU - Jongsma, M.A.. PY - 2003. Y1 - 2003. N2 - The mustard trypsin inhibitor, MTI-2, is a potent inhibitor of trypsin with no activity towards chymotrypsin. MTI-2 is toxic for lepidopteran insects, but has low activity against aphids. In an attempt to improve the activity of the inhibitor towards aphids, a library of inhibitor variants was constructed and cloned into the pRlac3 phagemid vector. The library of 9.3 107 independent colonies was created by randomisation of a stretch of five consecutive codons in the reactive site. Repeated selection rounds against bovine trypsin and chymotrypsin allowed the identification of novel, MTI-2 derived, antitrypsin and antichymotrypsin inhibitors. Chy8, the selected variant with highest affinity for bovine chymotrypsin (Ki = 32 nm versus ,1000 nm for the ...

The activity of proteases is inhibited by protease inhibitors.[18] One example of protease inhibitors is the serpin superfamily. It includes alpha 1-antitrypsin (which protects the body from excessive effects of its own inflammatory proteases), alpha 1-antichymotrypsin (which does likewise), C1-inhibitor (which protects the body from excessive protease-triggered activation of its own complement system), antithrombin (which protects the body from excessive coagulation), plasminogen activator inhibitor-1 (which protects the body from inadequate coagulation by blocking protease-triggered fibrinolysis), and neuroserpin.[19] Natural protease inhibitors include the family of lipocalin proteins, which play a role in cell regulation and differentiation. Lipophilic ligands, attached to lipocalin proteins, have been found to possess tumor protease inhibiting properties. The natural protease inhibitors are not to be confused with the protease inhibitors used in antiretroviral therapy. Some viruses, with ...

In this study, we focused on four glial proteins that are abundant in amyloid plaques and/or that are known to interact with Abeta: alpha1-antichymotrypsin (ACT), interleukin-1beta (IL-1beta), S100beta, and butyrylcholinesterase (BChE). We examined the ability of these proteins to activate rat cortical astrocyte cultures and to influence the ability of Abeta to activate astrocytes. Treatment of astrocytes with ACT, IL-1beta, or S100beta resulted in glial activation, as assessed by reactive morphology, upregulation of IL-1beta, and production of inducible nitric oxide synthase and nitric oxide. The ability of Abeta to induce astrocyte activation was also enhanced in the presence of each of these three proteins. In contrast, BChE alone did not activate astrocytes and had no effect on Abeta-induced activation ...

14.3.3 Pan, Acinus, AIF (Apoptosis Inducing Factor), Alpha-1-antichymotrypsin, alpha-1-antitrypsin, ARC, Ask1/MAPKKK5, ATM, BAG-1, Bak, Bax, Bcl10/CIPER/CLAP/mE10, bcl-2a, Bcl-6, bcl-X, bcl-XL, Bim (BOD), Caspase 1, Caspase 2, Caspase 3, Caspase 5, Caspase 6 (Mch 2), Caspase 7 (Mch 3), Caspase 8 (FLICE), Cathepsin D, CD137 (4-1BB), CD14, CD95/Fas, CDK2, CDK4, CDK5, c-fos, CIDE-A, CIDE-B, c-jun, CREB, CREB-Binding Protein, Cytochrome c, D4-GDI, Daxx, DcR1, DcR2/TRAIL-R4/TRUNDD, DFF40 (DNA Fragmentation Factor 40)/CAD, DFF45/ICAD, DR3, DR5, ERK1, ERK2, FADD (FAS-Associated death domain-containing protein), Fas-ligand, FLIP, Granzyme B, Heat Shock Protein 70/hsp70, I-FLICE/CASPER, IGF-1R, IGF-I, I-Kappa-B Kinase b (IKKb), JNK Activating kinase (JKK1), MADD, Mcl-1, Mekk-1, NF kappa B/p50, NF kappa B/p65 (Rel A), p53, PARP, PARP (Poly ADP-Ribose Polymerase), Prohibitin, RAIDD, SODD (Silencer of Death Domain), Survivin, TNF alpha, TNFa, ...

Armstrong RA, Winsper SJ, Blair JA (1995) Hypothesis: is Alzheimers disease a metal_induced immune disorder? Neurodegeneration 4:107_111 Bennett SA, Stevenson B, Staines WA, Roberts DC (1995) Periodic acid_Schiff (PAS)_positive deposits in brain following kainic acid_induced seizures: relationships to fos induction, neuronal necrosis, reactive gliosis, and blood_brain barrier breakdown. Acta Neuropathol Berl 89:126_138 Cras P, Smith MA, Richey PL, Siedlak SL, Mulvihill P, Perry G (1995) Extracellular neurofibrillary tangles reflect neuronal loss and provide further evidence of extensive protein cross_linking in Alzheimer disease. Acta Neuropathol Berl 89:291_295 Das S, Potter H (1995) Expression of the Alzheimer amyloid_promoting factor antichymotrypsin is induced in human astrocytes by IL_1. Neuron 14:447_456 De Simone R, Giampaolo A, Giometto B, Gallo P, Levi G, Peschle C, Aloisi F (1995) The costimulatory molecule B7 is expressed on human microglia in culture and in multiple sclerosis acute ...

Urticaria cholinergic is another type of the Urticaria that is caused by a certain physical stimulus of some or of the other class. It is classified like physical urticaria, meaning that is caused by a physical stimulus of a certain class. The physical stimulus could be indicated as heat although the real cause is defined as sweating. The study of the activity of the cell of the mast includes Urticaria generally cholinergic because most of the cases of Urticaria cholinergic looks like to critically imply the cells of the mast. The main mediator is the histamina of the serum, that rises in the concentration when an induced test exercise is introduced. This treatment also implies quimiotácticos factors eosinophil and of the neutrophil as well as it tryptase. The levels of the alpha 1-antichymotrypsin diminish, and the use of danazol gives rise to the improvement in the eruption. Due to these results, some have discussed in please proteases like a cause of the histamina that was sent. The skin ...

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I am 61 y.o. male having frequency urination for years. My Total PSA=4.4 and free PSA 11% ... or just monitor my PSA and do it after six months?

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Serpins: A family of serine proteinase inhibitors which are similar in amino acid sequence and mechanism of inhibition, but differ in their specificity toward proteolytic enzymes. This family includes alpha 1-antitrypsin, angiotensinogen, ovalbumin, antiplasmin, alpha 1-antichymotrypsin, thyroxine-binding protein, complement 1 inactivators, antithrombin III, heparin cofactor II, plasminogen inactivators, gene Y protein, placental plasminogen activator inhibitor, and barley Z protein. Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES, and some serpins occur in plants where their function is not known.

Polymorphonuclear neutrophils (PMN) play an important role in myocardial ischemia/reperfusion (MI/R) injury; however, the role of neutrophilic proteases is less understood. The effects of a novel serine protease inhibitor (serpin), LEX032, were investigated in a murine model of MI (20 min) and R (24 hr) injury in vivo. LEX032 is a recombinant human alpha 1-antichymotrypsin in which six amino acid residues were replaced around the active center with those of alpha-1 protease inhibitor. LEX032 has the ability to inhibit both neutrophil elastase and cathepsin G, two major neutral serine proteases in neutrophils, as well as superoxide generation. LEX032 (25 or 50 mg/kg) administered i.v. 1 min before reperfusion significantly attenuated myocardial necrotic injury evaluated by cardiac creatine kinase loss compared to MI/R rats receiving only vehicle (P , .001). Moreover, cardiac myeloperoxidase activity, an index of PMN accumulation, in the ischemic myocardium was significantly attenuated by LEX032 ...

Access Hybritech® free PSA Test,, PSA exists in multiple forms in the blood. Most is bound to proteins, but some is free-floating. In the early 1990s, it was discovered that measuring the ratio of free to total PSA could furth,medicine,medical supply,medical supplies,medical product

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Objectives: Compose a list toxins causing metabolic acidosis and hemodynamic instability Describe therapeutic interventions favoring aerobic metabolismin hemodynamically unstable patients Describe the mechanisms for paracetamol-inducedearly metabolic acidosis Outline the management of the...

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河南快36号开奖结果:The total PSA (prostate-specific antigen, prostate-specific antigen) value in serum of normal people is 10.0ng/ml, the probability of prostate cancer is very high. Between 4.0 to ...

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Objectives. Improved discrimination between prostate cancer (PC) and benign prostatic hyperplasia (BPH) is clearly needed. Our aim in this study was to evaluate whether the free to total prostate-specific antigen (PSA) ratio would be useful in the gray zone of 1.8-10 ng/mL total PSA range. Methods. In a consecutive series of 435 clinic patients referred for prostate evaluation, 308 had a total PSA ,10 ng/mL (92 had PC and 216 BPH). Free and total PSA were measured, and the free to total PSA ratio calculated. Results. Total PSA values were significantly different between the two groups. For the 200 patients with a total PSA ,6 ng/mL, no significant difference in total PSA values were seen (P = 0.411), whereas free to total PSA ratios remained statistically different (P , 0.001). Receiver operating characteristic (ROC) curve analysis comparing the performances of total PSA over the ratio of free to total PSA showed a clear advantage for the ratio at all sensitivity levels. Conclusions. These data ...

The mustard trypsin inhibitor, MTI-2, is a potent inhibitor of trypsin with no activity towards chymotrypsin. MTI-2 is toxic for lepidopteran insects, but has low activity against aphids. In an attempt to improve the activity of the inhibitor towards aphids, a library of inhibitor variants was constructed and cloned into the pRlac3 phagemid vector. The library of 9.3 x 107 independent colonies was created by randomisation of a stretch of five consecutive codons in the reactive site. Repeated selection rounds against bovine trypsin and chymotrypsin allowed the identification of novel, MTI-2 derived, antitrypsin and antichymotrypsin inhibitors. Chy8, the selected variant with highest affinity for bovine chymotrypsin (Ki = 32 nm versus |1000 nm for the wild-type) represents the strongest known recombinant chymotrypsin inhibitor of the MTI-2 family. It is highly toxic to nymphs of the aphid Acyrthosiphon pisum, and moderately toxic to nymphs of Aphis gossypii and Myzus persicae. The LC50 of 73 microg ml-1

Several PSA isoforms have been identified that can further increase the specificity of PSA for prostate cancer. In particular, the [-2] form of proPSA (p2PSA) shows improved performance over either total or free PSA for prostate cancer detection on biopsy. The prostate health index (phi) is a formula that combines all 3 PSA forms (total PSA, free PSA, and p2PSA) into a single score. Phi is calculated using the following formula: (p2PSA/free PSA) x square root (PSA).. In a multicenter study that compared the performance of PSA, free PSA, p2PSA, and phi in men undergoing prostate biopsy due to a serum PSA concentration between 4 and 10 ng/mL, phi was the best predictor of any prostate cancer, high-grade cancer, and clinically significant cancer. At 95% clinical sensitivity, the clinical specificity of phi was 16.0%, compared to 8.4% for free PSA and 6.5% for PSA.. Prostatic biopsy is required for diagnosis of cancer.. ...

How is it used?. If prostate cancer is diagnosed, the total PSA test may be used as a monitoring tool to help determine the effectiveness of treatment. It may also be ordered at regular intervals after treatment to detect recurrence of the cancer.. The total PSA test and digital rectal exam (DRE) may be used to screen both asymptomatic and symptomatic men for prostate cancer. If either the PSA or the DRE are found to be abnormal, then the doctor may choose to follow this testing with a prostate biopsy and perhaps imaging tests, such as an ultrasound. If the DRE is normal but the PSA is moderately elevated, the doctor may order a free PSA test to look at the ratio of free to total PSA. This can help to distinguish between prostate cancer and other non-cancer causes of elevated PSA. Since the total PSA test can be elevated temporarily for a variety of reasons, a doctor may order another PSA a few weeks after the first to determine if the PSA is still elevated.. Currently there is no consensus ...

Plays a role in setting standards of care for HIV infection and opportunistic diseases related to HIV/AIDS. Find information about the AACT, annual reports and structures and operations of the committees. ...

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Looking for serine proteinase inhibitor? Find out information about serine proteinase inhibitor. A substance bonded, taped, or dip-dried onto a solid propellant to restrict the burning surface and to give direction to the burning process. Explanation of serine proteinase inhibitor

TY - JOUR. T1 - Expression and Automated Purification of Acetoacetyl CoA Thiolase from Sunflower Cotyledon. AU - Dyer, James H.. AU - Becker, James. AU - Geraldo, Victor. AU - Giron, Mario. PY - 2006/4. Y1 - 2006/4. N2 - In the sunflower (Helianthus annuus L.) cotyledons, two distinguishable thiolase activities have been identified: acetoacetyl CoA thiolase (AACT), specifically active during oxidation of short chain acetoacetyl CoA, and 3-oxoacyl CoA thiolase (OACT), active towards short, medium, and long-chain acyl CoAs. The purpose of this research was to optimize the purification of the AACT expressed in bacteria. Escherischia coli (E. coli) with the full-length sunflower AACT cDNA cloned into the expression vector pBAD-His B was induced for production of the AACT using 0.2% arabinose. Optimizing the conditions for protein purification is often an extremely empirical process requiring several iterations of a basic protocol each with specific changes. This iterative process was simplified ...

The lack of sufficient sensitivity and specificity of PSA as a screening modality for the diagnosis of prostate cancer underscores the importance of improving its operating characteristics to considerably improve the quality of predicting individual ...

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