Development of Recombinant Vaccine against A(H1N1) 2009 Influenza Based on Virus-like Nanoparticles Carrying the Extracellular Domain of M2 Protein
Page contains details about virus-like nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Marginal zone (MZ) B cells are thought to be responsible for the first wave of Abs against bacterial Ags. In this study, we assessed the in vivo response of MZ B cells in mice immunized with viral particles derived from the RNA phage Qbeta. We found that both follicular (FO) and MZ B cells responded to immunization with viral particles. MZ B cells responded with slightly faster kinetics, but numerically, FO B cells dominated the response. B1 B cells responded similarly to MZ B cells. Both MZ and FO B cells underwent isotype switching, with MZ B cells again exhibiting faster kinetics. In fact, almost all Qbeta-specific MZ B cells expressed surface IgG by day 5. Histological analysis demonstrated that a population of activated B cells remain associated with the MZ, probably due to the elevated integrin levels expressed by these cells. Thus, both MZ and FO B cells respond with rapid proliferation to viral infection and both populations undergo isotype switching, but MZ B cells remain in the MZ and may be
SWISS-MODEL Template Library (SMTL) entry for 4qss. Structure of the bromodomain of human ATPase family AAA domain-containing protein 2 (ATAD2) in complex with N-Methyl-2-pyrrolidone (NMP)
chains in the Genus database with same CATH superfamily 2LXP C; 4TU4 A; 5CUB A; 4BW4 A; 2YQL A; 4QYL A; 4FIP A; 5FE4 A; 2E7O A; 2RFJ A; 4QSS A; 5LVR A; 2C2V S; 5E74 A; 5HLS A; 5C85 A; 2I8N A; 5FE9 A; 5KU3 A; 4O71 A; 5FH7 A; 1WEQ A; 5B79 A; 2IDA A; 1VD4 A; 4PPE A; 2YT5 A; 5ENJ A; 5HEN A; 4UIY A; 4QNS A; 5IGM A; 4O7A A; 5CQ4 A; 2LV9 A; 5CUC A; 5ENC A; 2MIQ A; 2PNX A; 3U5J A; 5FB1 A; 5HQ5 A; 5HM0 A; 5I7X A; 3ZYQ A; 2EGP A; 5EWD A; 4X2I A; 2YQD A; 3GV4 A; 5HQ6 A; 5LUU A; 2E6R A; 4A9F A; 5HEM A; 1T1H A; 4MSX A; 4N3W A; 3N9P A; 4TT6 A; 5C8G A; 5A82 A; 2KR4 A; 3N9M A; 1E4U A; 3S92 A; 1WEE A; 5IGN A; 5FE8 A; 5J0D A; 5K29 A; 4NR6 A; 4L7X A; 4LLB A; 4A9H A; 3D7C A; 5I83 A; 2YUR A; 4A4B A; 5ETB A; 2VJF A; 2WP1 A; 4A9N A; 3I2D A; 5TAB A; 4IOQ A; 5H1V A; 5G4R A; 4YYK A; 5KTU A; 4MEP A; 5E73 A; 4CLB A; 5A5P A; 4TKP B; 2LN0 A; 3FKM X; 2FSA A; 2CT2 A; 4QSQ A; 2YW8 A; 2VPB A; 5IBN A; 2L0B A; 5DKH A; 3U5M A; 2CKL A; 4HBW A; 1F68 A; 5CFW A; 2YW5 A; 4UYF B; 4YY6 A; 3ZYU A; 4UYF A; 4CUU A; 3MUK A; 4NR5 A; 1WEW A; ...
Allolevivirus is a genus of viruses, in the family Leviviridae. Enterobacteria serve as natural hosts. There are currently only two species in this genus including the type species Enterobacteria phage Qbeta. Group: ssRNA(+) Order: Unassigned Family: Leviviridae Genus: Allolevivirus Enterobacteria phage FI Enterobacteria phage Qbeta Viruses in Allolevivirus are non-enveloped, with icosahedral and Spherical geometries, and T=3 symmetry. The diameter is around 26 nm. Genomes are linear and non-segmented, around 4kb in length. The genome codes for 4 proteins. Entry into the host cell is achieved by adsorption into the host cell. Replication follows the positive stranded RNA virus replication model. Positive stranded rna virus transcription is the method of transcription. Translation takes place by suppression of termination. The virus exits the host cell by bacteria lysis. Enterobacteria serve as the natural host. Viral Zone. ExPASy. Retrieved 15 June 2015. ICTV. Virus Taxonomy: 2014 Release. ...
In the present study, we describe a therapy for cat allergy based on immunization with the recombinant cat allergen Fel d1 displayed on VLPs derived from the bacteriophage Qβ (Qβ-Fel d1). The therapy is characterized by three key features: (a) Qβ-Fel d1 is highly immunogenic and a single vaccination is sufficient for therapy; (b) Qβ-Fel d1 is essentially nonreactogenic; and (c) the effector mechanism of the therapy is based upon the induction of allergen-specific IgGs.. Qβ-VLPs consist of 180 subunits of a 14-kD coat protein. These VLPs elicit strong B cell responses as a result of their highly organized and repetitive structures (Jegerlehner et al., 2002a,b). This feature can be exploited to enhance the immunogenicity of self- and foreign antigens. Chemical coupling of antigens via a cysteine to surface lysine residues on the VLP renders these antigens equally repetitive and consequently immunogenic. Moreover, host cell RNA, a ligand for TLR3 and TLR7 (Kanzler et al., 2007), is ...
DACCOSIM NG is a co-simulation environment (in the sense of the FMI standard) able to offer fixed or variable step numerical integration methods (Euler, Adams-Bashforth, QSS) which takes advantage of the main functionalities of the FMI-CS 2.0 standard (interpolation of inputs and outputs, error estimation, backtracking, ...). The tool also offers two co-initialization methods, one of which is based on the Newton-Raphson algorithm.. The developments are done in Java requiring a JRE version 8. The code implicitly exploits the software bricks of the JavaFMI suite (bitbucket.org/siani/javafmi) but the installation is very simple since only two .jar files need to be placed on the host machine. Moreover, there is an installer for Windows. It should also be noted that it is very easy to exchange complete co-simulation models between several machines with DACCOSIM NG thanks to a proprietary data exchange format.. The input data of the platform is a set of binary codes called FMU according to the FMI ...
JPT Peptide Technologies is a DIN ISO 9001:2015 certified and GCLP compliant integrated provider of innovative peptide based catalog products and custom services.
Stoll, E., Wilson, K. J., Reiser, J., Weissmann, C. Revised amino-acid sequence of qbeta coat protein between positions 1 and 60 Journal of Biological Chemistry 1977 252:990-993 PMID:838709 ...
Miranda Castro QSS: Pathology Set - Pathology Set: $215 (save $10). Price includes 357 Pathology Cards, 6 CDs and study guidelines. And shipping: use FREESHIP40 on checkout. The Quick Study System by Gwynn Cadwallader is a beautiful learning tool specifically created to help homeopathic students, study groups and homeopaths prepare for the certification examination of the Council for
Creative Biostructure now can provide custom Mempro™ Acyl CoA binding protein (ACBP) production service based on virus-like particles.
Rich graphical models for real-world scene understanding encode the shape and pose of objects via high-dimensional, continuous variables. We describe a particle-based max-product inference algorithm which maintains a diverse set of posterior mode hypotheses, and is robust to initialization. At each iteration, the set of particle hypotheses is augmented via stochastic proposals, and then reduced via an optimization algorithm that minimizes distortions in max-product messages. Our particle selection metric is submodular, and thus efficient greedy algorithms have rigorous optimality guarantees. By avoiding the stochastic resampling steps underlying standard particle filters, we also avoid common degeneracies where particles collapse onto a single hypothesis. Our approach significantly outperforms previous particle-based algorithms in the estimation of human pose from images and videos, and the prediction of protein side-chain conformations ...
1. AAN QSS Init. Treatment PD (Jan 2002) Miyasaki JM, Martin W, Suchowersky O, Weiner WJ, Lang AE. Practice parameter: initiation of treatment for Parkinsons disease: an evidence-based review: Report of the Quality Standards Subcommittee of the American Academy of Neurology. Neurology 2002 Jan 8; 58(1):11-7. 2. AAN QSS PD Diag. (April 2006) Suchowersky O, Reich S, Perlmutter J, Zesiewicz T, Gronseth G, Weiner WJ, Quality Standards Subcommittee of the American Academy of Neurology. Practice parameter: diagnosis and prognosis of new onset Parkinson disease (an evidence-based review): report of the Quality Standards Subcommittee of the American Academy of Neurology. Neurology 2006 Apr 11; 66(7):968-75. 3. AAN QSS PD Dyskin (April 2006) Pahwa R, Factor SA, Lyons KE, Ondo WF, Gronseth G, Bronte-Stewart H, Hallet M, Miyasaki J, Stevens J, and Weiner WJ. Practice Parameter: Treatment of Parkinsons disease with motor fluctuations and dyskinesia (an evidence-based review): Report of the quality ...
Simplified Particle Input ConnEction Specification (SPICES) is a particle-based molecular structure representation derived from straightforward simplifications of the atom-based SMILES line notation. It aims at supporting tedious and error-prone molecular structure definitions for particle-based mesoscopic simulation techniques like Dissipative Particle Dynamics by allowing for an interplay of different molecular encoding levels that range from topological line notations and corresponding particle-graph visualizations to 3D structures with support of their spatial mapping into a simulation box. An open Java library for SPICES structure handling and mesoscopic simulation support in combination with an open Java Graphical User Interface viewer application for visual topological inspection of SPICES definitions are provided.
Mesothelin is found in other cancers for several years, said Yao, also a researcher in the Dan L. Duncan Cancer Center at BCM. However, we didnt know the role it played in pancreatic cancer: until she and her colleagues reported in this article. In fact, they found very high levels of mesothelin in 18 of 21 samples of patients pancreatic tissues compared to amounts found in nearby normal tissues. In studies of this protein in the lab, pancreatic cancer cell lines that produced high levels of mesothelin grew faster and spread more than those in which mesothelin levels were lower. Pancreatic cancer cells grew and spread faster in mice whose tumors expressed high levels of mesothelin than in those whose cancer did not, said the researchers, who conducted the studies in an immune deficient mouse. ...
Viral Biorealm Family}} ==Baltimore Classification== Leviviridae; Levivirus ==Higher Order Categories== Family: Leviviridae Genus: Levivirus Species: Enterobacteria phage BZ13, Enterobacteria phage MS2[4] ==Description and Significance== ssRNA positive-strand viruses, no DNA stage Levivirus is one of two known genera of the family Leviviridae, with Allolevivirus being the other. It replicates in only three bacteria genera: Escherichia, Pseudomonas, and Caulobacter. The virus attaches to the pili, sometimes the virion receptor site, and transiently exposes viral RNA while penetrating the cell [2]. The family Leviviridae is found to have one of the fastest known mutation rates, at 10-3 bp/replication, but also one of the smallest genomes at 4,268 nt which code for 4 protein subunits [3]. The distinguishing factor of levivirus is a cell-lysis protein coded within its genome, something that is absent in allelovirus. In 1976 the species Enterobacteria phage MS2 became the first organism ever to have ...
Talking to the Misinformed: How Politicians Communicate with Constituents Who Lack Accurate Information.. Party Polarization in Factual Beliefs: Evidence from Government Officials and the Mass Public (with Nathan Lee and Brendan Nyhan).. Evaluating New Approaches to Promoting Vaccination: A Field Experiment on Parents in Vermont (with Bridget Ahrens, Christine Finley, Shari Levine, and Brendan Nyhan).. Combating Conspiracy Theories about Disease Epidemics: An Experiment on Zika in Brazil (with Victoria Chi and Brendan Nyhan).. Misperceptions, Corrections, and the Structure of Political Opinions (with Caitlin Davies).. Decider in Chief? Public Misperceptions about Presidential Power (with Scott Clifford and Brendan Nyhan).. Factual (In)accuracy and Partisan Selective Exposure (with students in my QSS 30.08 seminar).. ...
Novavax is developing vaccines based on virus-like particle (VLP) technology developed at the University of Massachusetts Medical School (UMMS) in Worcester.
A crack in a high mineral-content material, like bone or a synthetic mineral/polymeric composite, generates ion gradients which can be utilized for active targeting and repair. Employing is technique, an active self-propelled particle-based detection, delivery, and repair strategy for cracks is designed by utilizing the damaged matrix itself as both the trigger and the fuel.
Gene expression is typically regulated by gene regulatory proteins that bind to the DNA. Experiments have shown that these proteins find their DNA target site via a combination of 3D diffusion in the cytoplasm and 1D diffusion along the DNA. This stochastic transport sets a fundamental limit on the precision of gene regulation. We derive this limit analytically and show by particle-based GFRD simulations that our expression is highly accurate under biologically relevant conditions ...
Fingerprint Dive into the research topics of High-throughput automated luminescent magnetic particle-based immunoassay to monitor human exposure to pyrethroid insecticides. Together they form a unique fingerprint. ...
To determine the prevalence of parvovirus 4 infection and its clinical and sociodemographic correlations in Finland, we used virus-like particle-based serodiagnostic procedures (immunoglobulin [Ig] G, IgM, and IgG avidity) and PCR. We found 2 persons with parvovirus 4 primary infection who had mild or asymptomatic clinical features among hepatitis C virus-infected injection drug users.
This thesis outlines adaptivity schemes for particle-based methods for the simulation of nearly incompressible fluid flows. As with the remeshing schemes used in mesh and grid-based methods, there is a need to use localized refinement in particle methods to reduce computational costs. Various forms of particle refinement have been proposed for particle-based methods such as Smoothed Particle Hydrodynamics (SPH). However, none of the techniques that exist currently are able to retain the original degree of randomness among particles. Existing methods reinitialize particle positions on a regular grid. Using such a method for region localized refinement can lead to discontinuities at the interfaces between refined and unrefined particle domains. In turn, this can produce inaccurate results or solution divergence. This thesis outlines the development of new localized refinement algorithms that are capable of retaining the initial randomness of the particles, thus eliminating transition zone ...
The presence of obstacles modifies the way in which particles diffuse. In cells it is observed that the mean-square displacement of biomolecules scales as a power law with exponent smaller than one. This behavior, called anomalous diffusion, is due to the presence of macromolecules playing the role of obstacles. We discuss the effect of fixed macroscopic obstacles on the time needed by particles to cross a strip and we consider both a diffusive and a ballistic regime. We find that in some regimes this residence time is not monotonic with respect to the size and the location of the obstacles. We discuss our results for particles performing random walks on a two dimensional strip considering also the effect of an exclusion rule. Results obtained in collaboration with A. Ciallella (Rome), O. Krehel (Eindhoven), A. Muntean (Karlstadt), R. van Santen (Eindhoven), and A. Sengar (Eindhoven) will be discussed.. ...
Dynamic multidimensional modeling of structure and activity in multispecies biofilm systems - a particle-based approach Electronic version of a poster presented at the Biocomplexity VI workshop (May 12-16, 2004, University of Indiana, Bloomington). This version contains several videos of simulations carried out with our biofilm modelling framework ...
A new type of imaging that does not require a lens and uses reconfigurable particle-based masks to take multiple shots of an object is being developed by resear
The gene that encodes the SERTs is SLC6A4. The promoter region of SLC6A4 has two well-known polymorphisms aptly named short and long corresponding to the number of repeats in the 5-HTT-linked polymorphic region (HTTLPR). The short variation of this promoter leads to less transcription of the SERT gene SLC6A4.[2] Further, studies have found that the short allele of HTTLPR is associated with changes in the brain structure such as reduced grey matter in the perigeniculate region surrounding Cg25 and in the amygdala, areas important for emotional processing and mood regulation.[3] Massive genetic analysis of autistic patients reveals that the S allele is present significantly more in patients with Autism than without.[4] Since the presence of the short-HTTLPR promoter results in fewer SERTs being produced, and SERTs function by reuptaking and thus limiting serotonin-induced signal transduction, it is not unexpected that over 30% of autistic individuals, who more commonly have the short-HTTLPR ...
The Obama administration says it is concerned that some states are restricting access by low-income people to costly, revolutionary drugs for a liver-wasting disease called hepatitis C.
The RNA world hypothesis is supported by RNAs ability to store, transmit, and duplicate genetic information, as DNA does. RNA can act as a ribozyme, a special type of enzyme. Because it can perform the tasks of both DNA and enzymes, RNA is believed to have once been capable of supporting independent life forms.[15] Some viruses use RNA as their genetic material, rather than DNA.[45] Further, while nucleotides were not found in experiments based on Miller-Urey experiment, their formation in prebiotically plausible conditions was reported in 2009;[22] the purine base known as adenine is merely a pentamer of hydrogen cyanide. Experiments with basic ribozymes, like Bacteriophage Qβ RNA, have shown that simple self-replicating RNA structures can withstand even strong selective pressures (e.g., opposite-chirality chain terminators).[46] Since there were no known chemical pathways for the abiogenic synthesis of nucleotides from pyrimidine nucleobases cytosine and uracil under prebiotic conditions, it ...
In Western nations, the Recovery approach has become a widely accepted philosophy and treatment concept in mental health. Yet, community understanding of the Recovery approach remains largely unexplored. This study aimed to investigate (i) peoples awareness of the principles underpinning the Recovery approach in mental health, and (ii) the treatment approaches people consider most important, and whether these align with the Recovery approach. To achieve these aims, a random sample of 1217 Australian adults participated in the National Social Survey (QSS) via telephone interview. Peoples experience with mental health services, the importance they place on various treatment approaches, and their awareness of principles underpinning the Recovery approach were assessed. Analyses were conducted using descriptive statistics. Most participants (94%) agreed that regardless of the severity of symptoms experienced and/or the mental illness diagnosis, being diagnosed with a mental illness means there is ...
Why would they need lysogeny-or even, how could they lysogenize? I cant speak for them all, but MS2 is a (+)-stranded ssRNA phage. On infection, the RNA can be directly translated into proteins that replicate the phage RNA and direct the synthesis of any capsid proteins required to form new phage particles. The replicase enzyme makes copies of both plus and minus strands, though the latter are only used as a template to make more (+) strand for both translation and packaging into phage. To lysogenize, there needs to be a DNA phase to the replication cycle and there isnt any such phase for MS2 ...
Analysis and computational tools and modeling capability for high-temperature electrolysis, low-temperature electrolysis, photoelectrochemical, and solar thermochemical technologies at Sandia National Laboratories
Assume a particle-based fundamental physics. Then the non-living things in the universe outnumber the living by many orders of magnitude. But here is a striking fact given a restricted compositionality like van Inwagens, Toners or mine on which all there are is in the universe are particles and organisms: the number of kinds of living things outnumbers the number of kinds of non-living things by several orders of magnitude. The number of kinds of particles is of the order of 100, but there are millions of biological species (they may not all correspond to metaphysical species, of course).. Counting by individuals, living things are exceptional. But counting by kinds, physical things are exceptional. Only a tiny portion of the universe is occupied by life. But on the other hand, only a tiny portion of the space of kinds of entities is occupied by non-life.. I am not sure what to make of these observations. Maybe it is gives some credence to an Aristotelian rather than Humean way of seeing the ...
Circulating tumor cells (CTCs) are shed from solid tumors and found at extremely low frequencies in the blood of patients in most cancers. A subset of these cells can seed and give rise to metastases, which is the primary cause of cancer-related mortality. Isolation and characterization of these cells from blood as a liquid biopsy can be a sensitive, non-invasive method for early detection, disease monitoring and therapy selection. CTCs can be found even at early disease stages in preclinical models and patients. There is increasing evidence that clusters of CTCs in blood are associated with higher metastatic potential; however, efficient isolation and interrogation of these rare clusters is challenging. In this study, we utilize an in-line rare cell enrichment platform developed by Becton Dickinson (BD), coupled with the BD FACSTM Influx cell sorter to rapidly isolate both single cells and clusters from blood. This platform utilizes magnetic particle-based depletion of unwanted leukocytes and ...
MCell (Monte Carlo Cell) is a program for simulating spatially resolved cell models using particle-based Monte Carlo algorithms. Biological processes at the cell level take place in small and often...
We have recently developed a simulation approach to calculate the equilibrium phase diagram of particle-based microphase formers. Here, this approach is used to calculate the phase behavior of the square-well-linear model for different strengths and ranges of the linear long--range repulsive component. The results are compared with various theoretical predictions for microphase formation. The analysis further allows us to better understand the mechanism for microphase formation in colloidal suspensions. ... [Read More] ...
We employ a spectrum of theoretical and numerical methods such as particle-based computer simulations, classical density functional theory, Langevin- and Fokker-Planck equations, continuum equations, and coarse-graining strategies. ...
VV B337 Title: Relationships between several particle-based stochastic reaction-diffusion models. Abstract: Particle-based stochastic reaction-diffusion models have recently been used to study a number of problems in cell biology. These methods are of interest when both noise in the chemical reaction process and the explicit motion of molecules are important. Several different mathematical models have been used, some spatially-continuous and others lattice-based. In the former molecules usually move by Brownian Motion, and may react when approaching each other. For the latter molecules undergo continuous time random-walks, and usually react with fixed probabilities per unit time when located at the same lattice site. As motivation, we will begin with a brief discussion of the types of biological problems we are studying and how we have used stochastic reaction-diffusion models to gain insight into these systems. We will then introduce several of the stochastic reaction-diffusion models, ...
Provide an easy-to-use program for modeling particle-based reaction-diffusion dynamics in cells Provides an expandable Java library for particle reaction-diffusion simulations
from Genetic Engineering News by Michael Tackett, Graeme Doran, Daniel Pregibon Particle-Based Approach Profiles Up to 68 miRNAs from Plasma, Serum, and Exosomes MicroRNA (miRNA) profiling has tremendous potential for the diagnosis and prognosis of a broad range of diseases including cancer, cardiovascular disorders,
My late 2013 13 rMBP has a really annoying mura in the lower right quadrant of the display. The original display was replaced under warranty when the...
Recently, Ge et. al. reported on an investigation of electric-field responsive nanoparticles for drug release. In their study, the authors utilized emulsion polymerization methods to encapsulate drug compounds in conductive polypyrrole (PPy) nanoparticles that are suspended in a hydrogel. The temperature sensitive hydrogel, which is a liquid at low temperature, and can be tailored to become a gel at body temperature, is used to localize the PPy nanoparticles after they are subcutaneously injected at the point of interest. The hydrogel further provides a biodegradable and biocompatible matrix for the conductive nanoparticles. The nanoparticles provide additional features including increased surface area for high drug loading, along with the small size (50-100 nm) that enables ease of passage and excretion through the circulatory system. Flourescein and daunorubicin compounds were selected to be loaded into PPy nanoparticle during synthesis, which after polymerization were completely encapsulated. ...
Assistant Professor. research • biography • lab members • publications • data and analysis tools. Biography:. Dennis Ko received a B.S. from Cornell University in 1997. His research career began in the lab of Dr. Jeffrey W. Roberts at Cornell University. Using a genetic screen of the bacterial initiation factor σ70 followed by biochemical characterization, he identified a surface on the protein important for transcriptional regulation by the phage Q protein and revealed a novel role for a sigma factor beyond transcription initiation.. His training continued from 1997-2005 in the MSTP at Stanford University. He completed both MD and PhD degrees but clearly saw that his future was in research aimed at understanding the biology of disease. His thesis project was carried out in the laboratory of Dr. Matthew P. Scott. Using a combination of biochemistry, cell biology, genetics and animal models, he pioneered a new subject in the lab examining how genetic alterations lead to the ...
Extensive studies have been undertaken to describe naive B cells differentiating into memory B cells at a cellular and molecular level. However, relatively little is known about the fate of memory B cells upon Ag re-encounter. We have previously established a system based on virus-like particles