TY - JOUR. T1 - Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging. T2 - A proof of concept study. AU - Renne, Julius. AU - Hinrichs, Jan. AU - Schönfeld, Christian. AU - Gutberlet, Marcel. AU - Winkler, Carla. AU - Faulenbach, Cornelia. AU - Jakob, Peter. AU - Schaumann, Frank. AU - Krug, Norbert. AU - Wacker, Frank. AU - Hohlfeld, Jens M.. AU - Vogel-Claussen, Jens. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Purpose: To evaluate oxygen-enhanced T1-mapping magnetic resonance (MR) imaging as a noninvasive method for visualization and quantification of regional inflammation after segmental allergen challenge in asthmatic patients compared with control subjects.Materials and After institutional review board approval, nine asthmatic Methods: and four healthy individuals gave written informed consent. MR imaging (1.5 T) was performed by using an inversion-recovery snapshot fast low-angle shot sequence before (0 hours) and 6 hours and 24 hours ...
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MicroRNAs are small non-coding RNAs that regulate gene expression at the post-transcriptional level. While they have been implicated in various diseases, the profile changes in allergen inhalation challenge are not clarified in human. We aimed to evaluate changes in the microRNA profiles in the peripheral blood of asthmatic subjects undergoing allergen inhalation challenge. Seven mild asthmatic subjects participated in the allergen inhalation challenge. In addition, four healthy control subjects (HCs) were recruited. MicroRNA profiles in peripheral blood samples (pre-challenge and 2 hours post-challenge) were measured by the NanoString nCounter assay to determine changes in miRNA levels as these asthmatic subjects underwent an allergen inhalation challenge. One common miRNA, miR-192, was significantly expressed in both comparisons; HCs vs. pre-challenge and pre- vs. post-challenge, showing that miR-192 was significantly under-expressed in asthmatics compared to HCs and decreased in post-challenge at an
Purified Native Bovine Bos d 11 (β-casein) Allergen Protein from Creative Biomart. Native Bovine Bos d 11 (β-casein) Allergen Protein can be used for research.
Arrow Scientific offers a wide range of smart tools to effectively manage and monitor HACCP systems within the food manufacturing industry. The product range includes allergen kits, bacteria tests, hygiene training, pH meters, sanitation verification, and thermometers.. Arrow Scientific provide allergen testing equipment such as Enzyme Linked Immunosorbent Assay (ELISA) based test kits, AgraQuant® Allergen ELISA Kits and AgraStrip® Allergen Test Strips.. AgraStrip® Allergen Test Strips are a lateral flow strip which aids in quick and simple detection of common allergens making it ideal for use in commercial kitchens, and production facilities. Kits are available for over 20 allergens, including the big 8; soy, eggs, milk, fish, wheat, shellfish, tree nuts and peanuts.. ELISA based test kits and AgraQuant® Allergen ELISA Kits are designed for use in the quantitative analysis of food allergens in laboratories.. ...
TY - JOUR. T1 - Removal of bronchoalveolar cells augments the late eosinophilic response to segmental allergen challenge. AU - Hunt, L. W.. AU - Gleich, G. J.. AU - Kita, H.. AU - Weiler, D. A.. AU - Schroeder, D. R.. AU - Vuk Pavlovic, Z.. AU - Sur, Sanjiv. PY - 2002. Y1 - 2002. N2 - Background: In patients with quiescent asthma, macrophages are the most prevalent cells recovered by bronchoalveolar lavage (BAL). Through activation via their FcεRII receptors or by acting as antigen-presenting cells, macrophages could, in theory, promote the late airway response to allergen. Objective: In order to investigate the importance of macrophages and other airway luminal cells in inducing the late airway response, a novel washout experiment was designed. Methods: Five patients with ragweed-allergic asthma underwent bronchoscopy and segmental bronchial challenge with either normal saline or short ragweed extract in two segments of one lung. In a third segment of the opposite lung, 12 successive BALs ...
Allergen Labeling. The age-old wine label is getting more complicated by the year what with Organics, Meritage, Import Certification - and the new allergen labeling requirements.. Allergen Labeling is on the way - On July 26, 2006, TTB began allowing allergen information on labels for beer, wine and spirits. For now and the next several months at least, allergen labeling is voluntary. But if you elect to make any reference to any of the eight major food allergens, you would need to comply with the interim rule, and you would probably need to get a new label approval. The major food allergens are: milk, egg, fish, shellfish, tree nuts, wheat, peanuts, soybeans, and their proteins. The interim rule does not seem to have any special treatment if producer is very small, or if the amount of the allergen is very small. By way of example, many wines contain fish proteins as a fining agent. The bottler is not required to say anything about these proteins under current rules, but if the bottler ...
p,Asthma is a chronic respiratory disease which is characterized by reversible bronchoconstriction, airway hyperresponsiveness, and airway inflammation. Allergen inhalation by sensitized atopic asthmatics enhances airway hyperresponsiveness and inflammation, providing a model to study mild asthma exacerbation. Airway inflammation can be measured non-invasively from airway secretions by sputum induction. Prior to starting this thesis, information on allergen-induced inflammation measured from sputum was limited. Furthermore, the pro- or anti-inflammatory effects of asthma therapies had not been investigated using this model of allergen-induced airway inflammation. The aim of this thesis was to first characterize the allergen-induced changes in sputum inflammatory cells and determine the repeatability of measurements of sputum inflammatory cells following allergen inhalation challenge. In addition, this thesis was aimed to investigate the pro- or anti-inflammatory effects of asthma therapies on ...
Allergen exposure leads to allergen sensitization in susceptible individuals and this might influence allergic rhinitis (AR) phenotype expression. We investigated whether sensitization patterns vary in a country with subtropical and tropical regions and if sensitization patterns relate to AR phenotypes or age. In a national, cross-sectional study AR patients (2-70 y) seen by allergists underwent blinded skin prick testing with a panel of 18 allergens and completed a validated questionnaire on AR phenotypes. 628 patients were recruited. The major sensitizing allergen was house dust mite (HDM) (56%), followed by Bermuda grass (26%), ash (24%), oak (23%) and mesquite (21%) pollen, cat (22%) and cockroach (21%). Patients living in the tropical region were almost exclusively sensitized to HDM (87%). In the central agricultural zones sensitization is primarily to grass and tree pollen. Nationwide, most study subjects had perennial (82.2%), intermittent (56.5%) and moderate-severe (84.7%) AR. Sensitization was
BACKGROUND: The cysteinyl leukotrienes (LTC4, LTD4 and LTE4) have been implicated in the pathogenesis of allergen-induced airway responses. The effects of pretreatment with BAYx 1005, an inhibitor of leukotriene biosynthesis via antagonism of 5-lipoxygenase activating protein, on allergen-induced early and late asthmatic responses has been evaluated. METHODS: Eight atopic subjects with mild asthma participated in a two period, double blind, placebo controlled, cross-over trial. Subjects were selected on the basis of a forced expiratory volume in one second (FEV1) of , 70% predicted, a methacholine provocative concentration causing a 20% fall in FEV1 (PC20) of , 32 mg/ ml, a documented allergen-induced early response (EAR, , 15% fall in FEV1 0-1 hour after allergen inhalation) and late response (LAR, , 15% fall in FEV1 3-7 hours after allergen inhalation), and allergen-induced airway hyperresponsiveness (at least a doubling dose reduction in the methacholine PC20 30 hours after allergen ...
Using a combination of in vitro and in vivo experiments and both artificial and natural allergen particles, we have presented evidence that the cross-linking of IgE/FcεRI complexes on MCs by particulate allergens induces not only MC degranulation but also phagocytosis of the activating allergens. Following phagocytosis, the allergens avoid intracellular degradation and acquire the potential for extracellular release. This remarkable phenomenon is directly attributable to lipid raft-mediated phagocytosis of allergen followed by the conversion of allergen-containing phagosomes into secretory compartments within the cells. Evidence that the IgE/FcεRI-mediated MC phagocytosis of particulate allergens involves lipid raft entities comes from the observations that: 1) the phagocytic event is preceded by the association of IgE/FcεRI complexes with lipid rafts; and 2) the disruption of lipid rafts in MCs blocked the phagocytosis of allergens. Lipid rafts have previously been reported to be essential ...
Expression of pulmonary eotaxin protein and mRNA was determined in six subjects with atopic asthma and five nonatopic normal subjects. Levels of eotaxin expression and eosinophil mobilization were compared before and after segmental allergen challenge in subjects with atopic asthma. In the absence o …
Researchers suspect that allergies to common inhaled allergens (such as house dust mite, cat dander, and grass pollens) are a major cause of childhood asthma. Recent evidence suggests that if allergies to inhaled allergens are prevented, this can cause changes in the immune system that may inhibit the development of asthma. Although strategies to prevent allergies generally focus on avoiding the allergen, complete avoidance of the common allergens linked to asthma would require extreme measures and is impractical.. Oral mucosal immunoprophylaxis (OMIP) therapy is an allergy treatment that can induce long-lasting immune tolerance in people already suffering from allergies. By exposing the patient to small, repeated, but increasing doses of the problem allergen over a long period of time, the patients immune system is eventually desensitized to that particular allergen. OMIP therapy has been shown to be safe in children as young as 2 years old. This study will evaluate if OMIP therapy against ...
Monotrace Food Allergen Elisa Kits Food Safety Biofront Technologies. BioFront Technologies introduces the first comprehensive line of monoclonal antibody-based enzyme-linked immuno-sorbent assay (ELISA) kits designed to accurately detect and quantify trace amounts food allergens in complex food matrices and surface rinses. Both approaches rapidly validate removal of potentially allergenic protein residues on food-contact surfaces. MonoTrace(R) ELISAs provide an unmatched union of specificity and sensitivity in food safety testing by incorporating precisely defined sets of monoclonal antibodies optimized to detect major allergens while minimizing cross-reactivity and food matrix interference. Be sure to browse our entire line of MonoTrace food allergen ELISA kits by clicking below or by downloading our product brochure. CLICK HERE TO DOWNLOAD OUR MONOTRACE FOOD ALLERGEN ELISA KITS BROCHURE TREE NUTS PEANUT MILK EGG GLUTEN SOY SEEDS SHELLFISH LUPIN
This course will discuss how food workers need to act, dress and be aware of food safety hazards and how they can prevent them. This course will provide you with the knowledge and skills to understand how GMPs support safe food manufacturing and your role in supporting GMPs. You will be able to identify food safety hazards and understand how food workers behavior and can have either a positive or a negative impact on food safety. This course will provide you with the knowledge and skills to understand how GMPs support the safe manufacturing of food and how to implement GMPs. *Become a member to receive 20% off ...
Analysis of a variety of allergenic foods has resulted in the identification of certain biochemical characteristics that are shared by most, but not necessarily all food allergens (16). One characteristic that appears to be shared by most food allergens is that they are extremely stable proteins resistant to denaturation. Resistance to denaturation of food allergens is thought to be an important characteristic because the longer significant portions of the protein remain intact the more likely it is to trigger an immune response (17). However, this property is not a predominant characteristic of aeroallergens, primarily because their route of sensitization is through the respiratory tract. The observation that many of the food allergens are proteins containing intramolecular disulfide bonds that may be important to their allergenicity (18) has led to the assumption that protein structure may be an important factor in an allergens ability to resist denaturation. This assumption was tested using ...
Kosher food manufacturers have stepped up their program to warn consumers about potential allergens. Even horseradish prepared at Brooklyns upscale Pomegranate carried a warning about nuts since equipment used to produce the horseradish presumably also was used to prepare foods containing nuts.. A review of some of the products on kosher shelves indicates that there were more warnings on allergens than ever before. One cautionary note came from a new Canadian study. We should narrow (various allergy labels) to only one which will be clear, said Dr. Moshe Ben-Shoshan, a professor of allergy and immunology at McGill University Health Center in Montreal, who worked on the study. An estimated 2.5 million Canadians and 15 million Americans suffer from food allergies, which can cause reactions ranging in severity from itchiness and vomiting to breathing problems, loss of consciousness and even death.. Allergy labels are governed in the U.S. by the Food Allergen Labeling and Consumer Protection Act ...
The AOAC Food Allergen Community has published its first newsletter for 2017, with a focus on reference materials for food allergen analysis. Contributing author Roland Poms from MoniQA Association describes the first validated set of reference materials, commercially released in January 2017. Katharina Scherf from the German Institute for Food Chemistry provides details about her investigations into gluten protein types from wheat, rye, barley and oats that can be used as reference materials.. As Editorial Board Member Bert Popping points out in his introductory comments, there are several factors that complicate the development of standardized reference materials for allergen analysis. For example, peanuts in USA and China can be significantly different in their composition, while internationally-standardised reference materials for allergen analysis must reflect what is currently traded on the market as a food ingredient.. While several food allergen reference materials have been developed ...
Allergy is a form of hypersensitivity to normally innocuous substances, such as dust, pollen, foods or drugs. Allergens are small antigens that commonly provoke an IgE antibody response. There are two types of bioinformatics-based allergen prediction. The first approach follows FAO/WHO Codex alimentarius guidelines and searches for sequence similarity. The second approach is based on identifying conserved allergenicity-related linear motifs. Both approaches assume that allergenicity is a linearly coded property. In the present study, we applied ACC pre-processing to sets of known allergens, developing alignment-independent models for allergen recognition based on the main chemical properties of amino acid sequences. A set of 684 food, 1,156 inhalant and 555 toxin allergens was collected from several databases. A set of non-allergens from the same species were selected to mirror the allergen set. The amino acids in the protein sequences were described by three z-descriptors (z 1
Allergen analysis is central to implementing and monitoring food allergen risk assessment and management processes by the food industry but current methods for determination of allergens in foods give highly variable results. The EU-funded iFAAM project has been working to address gaps in knowledge regarding food allergen management and analysis, including the development of novel mass spectrometry (MS) and immuno -based allergen determination methods. Common allergenic food ingredients (peanut, hazelnut, walnut, cows milk and hens egg) and common food matrices (chocolate dessert and cookie) have been used for both clinical studies and analytical method development to ensure that the new methods are clinically relevant. Allergen molecules have been used as analytical targets and allergenic ingredients incurred into matrices at levels close to reference doses that may trigger the use of precautionary allergen labelling. An inter-laboratory method comparison has been undertaken for the ...
TY - JOUR. T1 - IgE sensitization patterns to commonly consumed foods determined by skin prick test in Korean adults. AU - Kim, Sung Ryeol. AU - Park, Hye Jung. AU - Park, Kyung Hee. AU - Lee, Jae Hyun. AU - Park, Jung Won. PY - 2016. Y1 - 2016. N2 - Offending food allergens can vary with regional preferences in food consumption. In this study, we analysed sensitization rates to commonly consumed foods in Korean adults suspected of having food allergy. One hundred and thirty four subjects underwent a skin prick test (SPT) with 55 food allergens, of which 13 were made by our laboratory and the rest were commercially purchased. Of the 134 patients, 73 (54.5%) were sensitized to one or more food allergens. Sensitization to chrysalis was detected most frequently, at a rate of 25.4%. Sensitization rates to other food allergens were as follows: maize grain (13.4%), shrimp (11.9%), almond (11.1%), wheat flour (8.2%), lobster (8.2%), buckwheat (8.2%), mackerel (5.2%), pollack (5.2%), halibut (4.5%), ...
Detect possible allergic responses to various substances in the environment and evaluate for hay fever, asthma, atopic eczema, and respiratory allergy. The quantitative allergen-specific IgE test is indicated (1) to determine whether an individual has elevated allergen-specific IgE antibodies; (2) if specific allergic sensitivity is needed to allow immunotherapy to be initiated; (3) when testing individuals for agents that may potentially cause anaphylaxis; (4) when evaluating individuals who are taking medication (eg, long-acting antihistamines) that may interfere with other testing modalities (eg, skin testing); (5) if immunotherapy or other therapeutic measures based on skin testing results have not led to a satisfactory remission of symptoms; (6) when an individual is unresponsive to medical management where identification of offending allergens may be beneficial. ...
That exposure to high levels of house dust mite allergen was associated with deteriorating BHR was not an unexpected finding. House dust mite allergens include a number of proteases and trypsins that may disrupt cell membranes and have direct pro-inflammatory actions, and they also mediate airway inflammation through IgE activation [21]. They may therefore contribute to the persistence of airway inflammation over time, and perhaps a consequent increase in bronchial hyperresponsiveness.. Early-life exposure to dogs is associated with a reduced likelihood of developing asthma in children,[22] but there are no data for adults with asthma. It is interesting therefore that we have found an association between exposure to high levels of dog allergen and improvement of BHR. We considered the possibility that subjects may have gotten rid of their dog between visits, but even after controlling for this, we discovered that the association remained the same. It is also possible that dog ownership per se ...
Food allergens continue to be a significant issue for FSIS. From 2008 through 2013, there has been a sustained increase in the number of recalls of FSIS-regulated product that contained undeclared allergens. The proportion of recalls attributed to undeclared allergens and ingredients of public health concern has also increased, from 13% in 2008 to 35% in 2012. Furthermore, 14% of undeclared allergen recalls from 2000 through 2012 were the result of investigations following consumer complaints, 9 of which were associated with reports of allergic reactions.. The trend of allergen recalls in FDA-regulated products from fiscal year 2007 through 2012 follows a similar increasing trend over time, with the exception of fiscal year 20091. Approximately 12% of the 732 allergen recalls over the six year study period resulted from the report of a consumer reaction1.. In a 2001 study, of individuals with self-reported food allergies who recalled their last allergic reaction to food, approximately 50% ...
The mechanisms by which immune responses to nonpathogenic environmental antigens lead to either allergy or nonharmful immunity are unknown. Single allergen-specific T cells constitute a very small fraction of the whole CD4(+) T cell repertoire and can be isolated from the peripheral blood of humans according to their cytokine profile. Freshly purified interferon-gamma-, interleukin (IL)-4-, and IL-10-producing allergen-specific CD4(+) T cells display characteristics of T helper cell (Th)1-, Th2, and T regulatory (Tr)1-like cells, respectively. Tr1 cells consistently represent the dominant subset specific for common environmental allergens in healthy individuals; in contrast, there is a high frequency of allergen-specific IL-4-secreting T cells in allergic individuals. Tr1 cells use multiple suppressive mechanisms, IL-10 and TGF-beta as secreted cytokines, and cytotoxic T lymphocyte antigen 4 and programmed death 1 as surface molecules. Healthy and allergic individuals exhibit all three ...
The availability of allergen molecules (components) from several protein families has advanced our understanding of immunoglobulin E (IgE)-mediated responses and enabled component-resolved diagnosis (CRD). The European Academy of Allergy and Clinical Immunology (EAACI) Molecular Allergology Users Guide (MAUG) provides comprehensive information on important allergens and describes the diagnostic options using ... read more CRD. Part A of the EAACI MAUG introduces allergen molecules, families, composition of extracts, databases, and diagnostic IgE, skin, and basophil tests. Singleplex and multiplex IgE assays with components improve both sensitivity for low-abundance allergens and analytical specificity; IgE to individual allergens can yield information on clinical risks and distinguish cross-reactivity from true primary sensitization. Part B discusses the clinical and molecular aspects of IgE-mediated allergies to foods (including nuts, seeds, legumes, fruits, vegetables, cereal grains, ...
INCLUSION CRITERIA:. 1) Male or female, aged 18-65 years. (2) Asthmatic subjects with mild allergic asthma. (3) Positive skin prick test to cat allergen with a wheal diameter at least 2mm l. (4) RAST score of at least 1 or equivalent international units/mL.. (5) Subjects must express one (or more) of the following Human Leukocyte Antigens (HLA): HLA-DRB1*0101, HLA-DRB1*0301, HLA-DRB1*0401, HLA-DRB1*0405, HLA-DRB1*0701, HLA-DRB1*0901, HLA-DRB1*1001, HLA-DRB1*1101, HLA-DRB1*1301, HLA-DRB1*1401, HLA-DRB5*0101.. (6) Methacholine provocative concentration causing a 20% fall in the FEV1 (PC20) , 0.125 mg/ml and ,16 mg/mL.. (7) Development of early asthmatic response (≥20% fall in FEV1) with or without a late phase asthmatic response during a screening whole lung allergen challenge.. (8)Willing and able to provide written informed consent. (9) The subject must be willing and able to comply with the study requirements. (10) If the subject is female and of childbearing potential she must practice an ...
SDAP (Structural Database of Allergenic Proteins) is a Web server that provides database information and various cobputational tools for the study of allergenic proteins. The database component of SDAP contains information the allergen name, source, sequence, structure, IgE epitopes, and literature references, and links to the major protein (PDB, SWISS-PROT, PIR, NCBI) and literature (PubMed, MEDLINE) servers. The computational component in SDAP uses an original algorithm based on conserved properties of amino acid side chains to identify regions of known allergens similar to user-supplied peptides or selected from the SDAP database of IgE epitopes. This and other bioinformatics tools can be used to rapidly determine potential cross-reactivities between allergens and to screen novel proteins for the presence of IgE epitopes they may share with known allergens. SDAP was developed guided by the allergens list from the IUIS (International Union of Immunological Societies) website, ...
A healthy home begins with cleaner air. Improve the air quality in your house by reducing the amount of airborne allergens with a Filtrete™ Allergen Defense Micro Allergen Filter. Using electrostatic technology, this high-performance furnace filter turns your central heating and cooling system into a whole-house air cleaner by attracting and capturing microscopic allergens like pet dander, smoke and household dust, while also taking care of larger airborne allergens such as mould spores and pollen. For maximum performance, replace your Filtrete™ Filter seasonally (every 90 days) to ensure you and your household are breathing cleaner air.. ...
A radioimmune method based on detection of IgE antibodies (RAST) has been used for allergen quantitation, both as a direct test and as an inhibition test. Commercially available allergen extracts from birch pollen, timothy pollen and horse dandruff were studied. Variations of the allergen concentration up to a thousandfold were found between some extracts and these differences were confirmed by the results obtained with the chopped human lung test and in provocation tests. The use of RAST for estimation of allergen concentrations seems to be of great value, since the method combines easy performance with high capacity and independence of allergic patients as test objects. The same set of test sera can be used for several years, ensuring standardized conditions.. ...
Figure 4. Contact dermatitis to gold is seen in areas of skin where eye makeup, foundation or sunscreens containing harder metals (eg, titanium, zinc) are applied.. Conclusion. The Allergen of the Year is an annual award of dubious distinction voted upon by members of the ACDS. When Ponciano Cruz, MD, created the section, the original intent was to call attention to individual allergens and specifically draw attention to allergens that are very common, underrecognized, merit more attention (because they are causing significant ACD) and are no longer causing significant relevant disease (as in thimerosal in the United States). The core intent is academic in nature - to highlight societal and public awareness. Nominations for the Allergen of the Year are solicited from the ACDS, and final determination is made through a committee, chaired by Donald Belsito, MD. Afterwards, an expert on the allergen is selected to review and author a manuscript on the allergen for publication in Dermatitis. ...
Immunoglobulin E (IgE)-mediated allergy occurs when our immune system causes a reaction to otherwise harmless substances (allergens). Allergens are predominantly proteins present in biological materials such as pollens, mites, animal epithelia, moulds and foods. In vitro tests for specific IgE antibodies usually employ an allergen source extract as an antibody capturing reagent. The proportion of allergenic molecules in these biochemically complex extracts may vary.. Recombinant allergens may be obtained in large quantities with biotechnological techniques. These proteins can be characterized biochemically and immunologically, resulting in tests with minimal batch-to-batch variation. This thesis describes different uses of recombinant allergens in component-resolved diagnostics (CRD).. In CRD, single allergenic proteins are used to establish a sensitization profile of the patient. Two timothy grass (Phleum pratense) pollen allergens, Phl p 11 and Phl p 4, were cloned and expressed as recombinant ...
All chefs, cooks and staff must be aware of the food allergens contained in their prepared foods. Here at Stephensons catering equipment suppliers, we stock and supply a wide range of innovative food allergy labels and allergen notices for staff and customers. View our complete allergen signage and labels for stickers, skewers and wall/table notices.. For more information about new allergen law and for advice on the best allergen notice systems and products contact our Customer Services team on 0161 483 6256. ...
Egg proteins represent one of the most important sources evoking food allergic reactions. In order to improve allergy diagnosis, purified and well-characterized proteins are needed. Although the egg white allergens Gal d 1, 2, 3 and 4 (ovomucoid, ovalbumin, ovotransferrin, and lysozyme) are commercially available, these preparations contain impurities, which affect exact in vitro diagnosis. The aim of the present study was to set up further purification protocols and to extend the characterization of the physicochemical and immunological properties of the final batches. The egg white allergens Gal d 1-4 were purified from commercial preparations, whereas Gal d 5 (a-livetin) was purified from egg yolk. The final batches of Gal d 1-5 consisted of a range of isoforms with defined tertiary structure. In addition, the IgE binding capacity of the purified egg allergens was tested using allergic patients sera. The allergen batches will be further used to set up allergen specific diagnostic assays and ...
Due to the amount of air a whole house fan moves when properly sized to the home, dust does not have any time to settle in the home! Most customer, even in the dustiest of area, do not notice a big increase in the amount of dust in the home.. As well, because the fan moves so much air, it is taking any allergens that may be inside or outside the home and exhausting it so fast they have no chance to circulate inside of the home. The fan sucks any allergens into the attic and then exhausts it through the attic vents. In fact, the allergens inside the home can be much worse than the allergens outside of the home. We even have some customers who have very bad allergies state that their allergies almost went away after installing the QuietCool!. For customers that are still concerned with bringing in dust or allergens, we have a new product to help. We have partnered with PollenTec, a manufacturer of window screens that block 99.9% of dust and allergens from entering the home. The screens are very ...
TY - JOUR. T1 - Effects of overexpression of IL-10, IL-12, TGF-β and IL-4 on allergen induced change in bronchial responsiveness. AU - Fu, Chi Ling. AU - Ye, Yi Ling. AU - Lee, Yueh L.. AU - Chiang, Bor Luen. PY - 2006/5/8. Y1 - 2006/5/8. N2 - Background: An increasing prevalence of allergic diseases, such as atopic dermatitis, allergic rhinitis and bronchial asthma, has been noted worldwide. Allergic asthma strongly correlates with airway inflammation caused by the unregulated production of cytokines secreted by allergen-specific type-2 T helper (Th2) cells. This study aims to explore the therapeutic effect of the airway gene transfer of IL-12, IL-10 and TGF-β on airway inflammation in a mouse model of allergic asthma. Methods: BALB/c mice were sensitized to ovalbumin (OVA) by intr aperitoneal injections with OVA and challenged by nebulized OVA. Different cytokine gene plasmids or non-coding vector plasmids were instilled daily into the trachea up to one day before the inhalatory OVA ...
TY - CHAP. T1 - Validation by Mass Spectrometry Food Allergen Analysis: Detection, Quantification and Validation by Mass Spectrometry. AU - Planque, Mélanie. AU - Arnould, Thierry. AU - Gillard, Nathalie. PY - 2017/10/4. Y1 - 2017/10/4. N2 - Worldwide, food-allergy-related diseases are a signi cant health problem. While the food industry works on managing cross-contaminations and while clinicians deal with treatment, laboratories must develop e cient analytical methods to ensure detection of hidden allergens that can cause severe adverse reactions. Over the past few years, huge progress has been made in mass spectrometry for the analysis of allergens in incurred and processed foodstu s, especially as regards sample preparation and enrichment (solid phase extraction, protein precipitation and ultra ltration). These achievements make it possible to meet the Allergen Bureaus Voluntary Incidental Trace Allergen Labelling (VITAL) sensitivity criteria. The present chapter details the di erent steps ...
Testing for IgE antibodies may be useful to establish the diagnosis of an allergic disease and to define the allergens responsible for eliciting signs and symptoms.. Testing also may be useful to identify allergens which may be responsible for allergic disease and/or anaphylactic episode, to confirm sensitization to particular allergens prior to beginning immunotherapy, and to investigate the specificity of allergic reactions to insect venom allergens, drugs, or chemical allergens.. ...
Ambient inhalable particulate matter acts as an efficient carrier of allergens allowing penetration deep into the distal airways. Epidemiological studies have shown an association between the increased incidence of asthma and levels of ambient particulate matter from air pollutants. Most experimental models investigating the pathogenesis of allergic asthma have used soluble allergens (sAgs) whereas knowledge is limited regarding the responses evoked by particulate allergens (pAgs). In this study, pathological responses to sAgs were compared with the same amount of allergens adsorbed on to the surface of polystyrene particles, in sensitised mice.. Regardless of allergen type, airways hyper-responsiveness and eosinophil infiltration were significantly greater with pAgs compared with sAgs. The response was blunted in mast cell (MC)-deficient mice suggesting that MCs are crucial in this response. MCs were able to distinguish between pAgs and sAgs. A fivefold greater MC IgE receptor-mediated response ...
Health,Common allergens (such as dust mite and grass pollen) and viruses may ...Sixty patients (aged 17 to 50) admitted to hospital over a year wit...A significantly higher proportion of patients admitted with asthma ...Viruses were detected in 26% of patients admitted with asthma compa...Allergens and viruses may act together to exacerbate asthma indica...,Allergens,and,viruses,act,together,to,worsen,asthma,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
AllerGen investigator and pediatric allergist Dr. Edmond S. Chan (The University of British Columbia & BC Childrens Hospital) is the winner of the 2019 Robyn Allen Leadership Award.. The award is presented by AllerGen Legacy Partner Food Allergy Canada (FAC) to recognize an individual who has made a unique contribution to the lives of Canadians with food allergy through his or her efforts in education, advocacy, community building, leadership, or fundraising.. AllerGen NCE was also a nominee for this years award, for its contributions to the understanding of food allergy, anaphylaxis and other related conditions through its research efforts, which have important implications for national food allergy management policy and practice.. Dr. Chan is a clinician-scientist interested in food allergy prevention, diagnosis, and management who has contributed to AllerGens NFASt research portfolio and who is involved in the National Food Allergy Action Plan championed by AllerGens Legacy Partners ...
The most common allergens include: House dust mites House dust mites, which are part of the spider family, measure between 0.2-0.4mm and are prevalent in all households, where they tend to be more numerous in bedding, upholstery and carpets). High levels of exposure to house dust mite allergens can occur throughout the day in a range of indoor environments both inside and
Specific allergy vaccination is an efficient treatment for allergic disease; however, the development of safer vaccines would enable a more general use of the treatment. Determination of molecular structures of allergens and allergen-Ab complexes facilitates epitope mapping and enables a rational approach to the engineering of allergen molecules with reduced IgE binding. In this study, we describe the identification and modification of a human IgE-binding epitope based on the crystal structure of Bet v 1 in complex with the BV16 Fab fragment. The epitope occupies approximately 10% of the molecular surface area of Bet v 1 and is clearly conformational. A synthetic peptide representing a sequential motif in the epitope (11 of 16 residues) did not inhibit the binding of mAb BV16 to Bet v 1, illustrating limitations in the use of peptides for B cell epitope characterization. The single amino acid substitution, Glu(45)-Ser, was introduced in the epitope and completely abolished the binding of mAb ...
An allergy is a state of over-reactivity or hypersensitivity of the immune system of a pet to a particular substance called an allergen. Most allergens are proteins. The allergen protein may be of insect, plant or animal origin. Initial exposure of the dog, or more likely multiple exposures, to the allergen may over-sensitize the immune system, such that a subsequent exposure to the same or related allergen causes an over-reaction. This means that the immune response, which normally protects the dog against infection and disease, can be harmful. The actual immune reactions involved in allergies are quite complex. Most reactions involve an antibody in the blood called Immunoglobulin E (IgE). In an allergic reaction the allergen protein molecules combine with IgE antibody molecules and attach to a type of cell called mast cells, found in many tissues. When these cells are attached to the allergen, they break up and release potent chemicals such as histamines, which cause local inflammation. This ...
The National Asthma Education and Prevention Program Expert Panel guidelines for the management of asthma recommend that patients who require daily asthma medications have allergy testing for perennial indoor allergens and that, when triggers are found, exposure to allergens and pollutants be controlled through avoidance and abatement. For patients whose symptoms are not controlled adequately with these interventions and who are candidates for immunotherapy, the guidelines recommend referral to an allergist. However, the data supporting these recommendations are not consistent. Although there is evidence that simple allergen avoidance measures are ineffective, there is good evidence for the effectiveness of a comprehensive approach based on known sensitization. Thus, allergen avoidance may include removal of pets, use of high-efficiency particulate air filtration and vacuum cleaners, use of allergen-impermeable mattress and pillow covers, cockroach extermination, smoking cessation, and measures to
Resistance to proteolytic enzymes and heat is thought to be a prerequisite property of food allergens. Allergens from peanut (Arachis hypogaea) are the most frequent cause of fatal food allergic reactions. The allergenic 2S albumin Ara h 2 and the homologous minor allergen Ara h 6 were studied at the molecular level with regard to allergenic potency of native and protease-treated allergen. A high-resolution solution structure of the protease-resistant core of Ara h 6 was determined by NMR spectroscopy, and homology modelling was applied to generate an Ara h 2 structure. Ara h 2 appeared to be the more potent allergen, even though the two peanut allergens share substantial cross-reactivity. Both allergens contain cores that are highly resistant to proteolytic digestion and to temperatures of up to 100 °C. Even though IgE antibody-binding capacity was reduced by protease treatment, the mediator release from a functional equivalent of a mast cell or basophil, the humanized RBL (rat basophilic ...
Polymorphism is a frequent feature for allergens molecules. Different degrees of polymorphism have been described in the allergens of many different sources, including house dust mites [26], foods [27, 28] and allergens from different grass and tree pollens [29, 30]. However, although allergen polymorphism is beginning to be deeply characterized at the molecular level, little is known in many cases regarding the origin of such polymorphism. In several cases, allergen polymorphism has been attributed to the presence of multigene protein families [31]. In other allergens, the presence of post-translational modifications may also determine the presence of multiple forms of the allergen, as is the case of Ole e 1 [32]. In apple (Malus domestica), up to 18 Mal d 1 genes have been characterized. Allelic diversity regarding this allergen has been considered as a major explanation for the considerable differences in allergenicity, widely described among apple cultivars [28]. The high rate of nucleotide ...
Hui Zoi et al. Correlation between fungal sIgE and bronchial asthma severity. Experimental and Therapeutic Medicine 2013;6:537-41.. The World Health Organization (WHO) has officially recognized 120 allergens from fungi (molds). Several hundred additional fungal proteins believed to be allergenic (allergy causing) have also been described. Fungal allergens most commonly trigger type I immediate type hypersensitivity reactions such as asthma, rhinitis and anaphylaxis. Fungal allergens can also trigger type III hypersensitivity reactions (such as hypersensitivity pneumonitis) and type IV hypersensitivity reactions (allergic bronchopulmonary aspergillosis [ABPA] and hypersensitivity pneumonitis). ...
The Nasal Allergen Challenge (NAC) model allows the study of Allergic Rhinitis (AR) pathophysiology and the proof of concept of novel therapies. The Allergic Rhinitis - Clinical Investigator Collaborative (AR-CIC) aims to optimize the protocol, ensuring reliability and repeatability of symptoms to better evaluate the therapies under investigation. 20 AR participants were challenged, with 4-fold increments of their respective allergens every 15 minutes, to determine the qualifying allergen concentration (QAC) at which the Total Nasal Symptom Score (TNSS) of ≥10/12 OR a Peak Nasal Inspiratory Flow (PNIF) reduction of ≥50% from baseline was achieved. At the NAC visit, the QAC was used in a single challenge and TNSS and PNIF were recorded at baseline, 15 minutes, 30 minutes, 1 hour, and hourly up to 12 hours. 10 additional ragweed allergic participants were qualified at TNSS of ≥8/12 AND ≥50% PNIF reduction; the Cumulative Allergen Challenge (CAC) of all incremental doses was used during the NAC
Abstract Introduction: Field studies for allergic rhinitis (AR) commonly have inconsistent allergen concentrations and subject exposure patterns due to varying environmental conditions and subject behaviors. A technical and clinical validation study was conducted for the Allergen BioCube® using timothy grass to confirm uniform allergen concentration and clinically relevant subject symptom responses. Methods: Allergen concentrations were verified by laser particle counts. Subjects (N = 14) with positive skin test reactions and no symptoms at screening received four 3‐h timothy grass exposures in the BioCube over consecutive days. Subjects evaluated nasal itching, sneezing, rhinorrhea, and nasal congestion while in the BioCube; Total Nasal Symptom Score (TNSS) was computed. Peak Nasal Inspiratory Flow (PNIF), Peak Expiratory Flow Rate (PEFR), sIgE blood tests, and Nasal Inflammation Score (NIS) were assessed. A correlation analysis was conducted for mean sIgE, skin test, and TNSS. Results: ...
An allergen-specific immunoglobulin E (IgE) blood test is done to check whether a person is allergic to a particular substance.. An allergic reaction occurs when the immune system overreacts to something, often in the environment, thats harmless to most people. To protect the body from this perceived threat, or allergen, the immune system of an allergic person produces antibodies called immunoglobulin E.. IgE antibodies are found mostly in the lungs, skin, and mucous membranes. They cause mast cells (a type of cell involved in the bodys immune response) to release chemicals, including histamine, into the bloodstream. Its these chemicals that bring on many of the allergy symptoms that affect a persons eyes, nose, throat, lungs, skin, or gastrointestinal tract.. Because IgE antibodies are unique to each allergen (for example, IgE produced in response to pollen differs from IgE produced after a bee sting), checking for specific variants in the blood can help determine if an allergy is ...
We are very attentive to cross-contamination issues and with all of our products we implement allergen-conscious storage controls, and wash and sanitize any utensils that are used in processing products with different allergen profiles. Our Quality Control team conducts Allergen Changeover Inspections between runs of any products with different allergens. This inspection includes a visual check of all equipment and utensils as well as a test for organic material residue. There is a small possibility that our products could come in contact with airborne particles of potential allergens in our facility, but we do take all necessary precautions to ensure that there is no direct contact between products with different allergen profiles. Our facility does not contain fish, shellfish, peanuts, treenuts, or eggs.. Back to Top ...
Ara h 1, a major peanut allergen, is known as a stable trimeric protein. Nevertheless, upon purification of native Ara h 1 from peanuts using only size exclusion chromatography, the allergen appeared to exist in an oligomeric structure, rather than as a trimeric structure. The oligomeric structure was independent of the salt concentration applied. Subjecting the allergen to anion exchange chromatography induced the allergen to dissociate into trimers. Ammonium sulfate precipitation did not bring about any structural changes, whereas exposing the allergen to hydrophobic interaction chromatography caused it to partly dissociate into trimers, with increasing amounts of trimers at higher ionic strengths. The (partial) dissociation into trimers led to a change in the tertiary structure of the monomeric subunits of the allergen, with the monomers in Ara h 1 oligomers having a more compact tertiary structure compared with the monomers in Ara h 1 trimers. As structural characteristics are important for ...
The complete mechanisms of allergen immunotherapy are not clear and remain the subject of investigation. The allergic reaction is dependent on the presence of allergen-specific immunoglobulin E (IgE) antibodies that are bound to specific receptors on mast cells and basophils. The presence of IgE antibodies sensitizes these cells, and upon interaction with the appropriate allergens, histamine and other mediators are released which produce local or systemic responses in sensitive individuals, and characteristic symptoms of atopic diseases, such as allergic rhinitis and allergic asthma. Changes in serum antibody and T-lymphocyte responses resulting from immunotherapy have been demonstrated, and these changes often correlate closely with clinical (symptom) improvements. Specific mechanisms may vary depending on the nature of the allergic disease, the allergenic specificities of patients and populations, extract formulations, route of administration, dose and duration of treatment.². Subcutaneous ...
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Background and Objectives: Anaphylaxis, a severe allergic disease, can be triggered by various causes. This study investigated the association between allergic sensitization and anaphylactic symptoms and the severity of anaphylaxis in children.Patients and Methods: A retrospective review of 107 pediatric patients with anaphylaxis was performed between January 1, 2015, and December 31, 2017; 74 patients underwent allergen testing for specific immunoglobulin E. Allergic sensitizations and associations with anaphylactic symptoms and severity were investigated.Results: Overall, 64 (86.5%) patients were sensitized to food or inhalant allergens. In children under 2 years of age, 90.5% were sensitized to food (P = 0.001); in those over 6 years of age, 84.6% were sensitized to inhalant allergens (P = 0.001). Milk sensitization was significantly associated with severe anaphylaxis (P = 0.036). The following symptoms showed significant associations with certain allergen sensitizations: facial edema with food;
Allergic diseases represent a significant burden in industrialized countries, but why and how the immune system responds to allergens remain largely unknown. Because many clinically significant allergens have proteolytic activity, and many helminths express proteases that are necessary for their life cycles, host mechanisms likely have evolved to detect the proteolytic activity of helminth proteases, which may be incidentally activated by protease allergens. A cysteine protease, papain, is a prototypic protease allergen that can directly activate basophils and mast cells, leading to the production of cytokines, including IL-4, characteristic of the type 2 immune response. The mechanism of papains immunogenic activity remains unknown. Here we have characterized the cellular response activated by papain in basophils. We find that papain-induced IL-4 production requires calcium flux and activation of PI3K and nuclear factor of activated T cells. Interestingly, papain-induced IL-4 production was dependent
AllerGen is pleased to announce that Dr. Catherine Biggs has been awarded the prestigious AllerGen Emerging Clinician-Scientist Research Fellowship.. During the two-year Fellowship, Dr. Biggs will train under the supervision of AllerGen investigator Dr. Stuart Turvey, pediatric immunologist and director of clinical research at BC Childrens Hospital, and professor at The University of British Columbia.. This is the fourth such fellowship that AllerGen has awarded since 2011. Valued at $250,000, these awards enable newly trained Canadian allergists and immunologists to advance their research expertise and pursue a combined career as clinicians and academic researchers.. Bringing Dr. Biggs back to Canada with this important AllerGen Fellowship is a big win for the Canadian clinical immunology community, says Dr. Turvey.. Read the press release. ...
Allergen Awareness. Food allergy is an immune system response to a protein in food that the body mistakenly believes is harmful. A food allergy reaction can potentially be life threatening.. Food labelling rules in Australia state that the ten most common declared allergens (peanut, tree nuts, milk, egg, fish, shellfish, wheat, sesame, soy and lupins) must be declared on food packaging.. The law in Australia requires food businesses to provide accurate information when a customer asks about potential allergens in the food they are served or sold.. This non-accredited Allergen Awareness course has been designed to assist owners and staff in any food business to gain knowledge about food allergens and develop best practice procedures to minimise the risks for customers with food allergies.. This is not an accredited training course. Training is delivered by Master Grocers Australia - RTO Code: 21148. ...
Allergic rhinitis is an inflammatory disease of the nasal mucosal membranes that causes sneezing, rhinorrhea, nasal pruritis and congestion. Patients who have seasonal rhinitis (hay fever) exhibit symptoms at specific times during the year, while patients who have perennial rhinitis have symptoms all year. Seasonal exacerbations of perennial rhinitis can also occur. Tree, grass and weed pollens are common seasonal allergens. Important perennial allergens include house dust mites, indoor molds, animal dander and occupational allergens.. Lee and Arriola discuss three therapies: (1) allergen avoidance, (2) pharmacologic treatment to prevent and control symptoms and (3) allergen immunotherapy for recalcitrant patients. Allergen avoidance is accomplished through environmental control aimed at reducing exposure to potential allergens. This may require patients to stay indoors as much as possible during times when the offending allergen is at its seasonal peak. Other measures include enclosing ...
The Respiratory Allergen Panel Region 2 is a great way to test for common allergens in your area. Request A Test is a convenient way to order affordable nationwide allergy testing.
Allergen immunotherapy, also known as desensitization or hypo-sensitization, is a medical treatment for some types of allergies. It is useful for environmental allergies, allergies to insect bites, and asthma. Its benefit for food allergies is unclear and thus not recommended. Immunotherapy involves exposing people to larger and larger amounts of allergen in an attempt to change the immune systems response. Meta-analyses have found that injections of allergens under the skin are effective in the treatment in allergic rhinitis in children and in asthma. The benefits may last for years after treatment is stopped. It is generally safe and effective for allergic rhinitis, allergic conjunctivitis, allergic forms of asthma, and stinging insects. The evidence also supports the use of sublingual immunotherapy against rhinitis and asthma, but it is less strong. In this form the allergen is given under the tongue and people often prefer it to injections. Immunotherapy is not recommended as a stand-alone ...
Background. Several studies have investigated levels of T-cell-derived interleukin (IL)-10 in individuals with atopic dermatitis, with conflicting results. Aims/Hypothesis. In order to address whether stratification of disease severity may help resolve the different findings, the hypothesis was tested that individuals with severe atopic dermatitis have a lower frequency of circulating IL-10-producing, allergen-specific CD4+ T cells than do individuals with mild disease. Methods. Using peripheral blood mononuclear cells derived from individuals with severe (n = 12) and mild atopic dermatitis (n = 10) and from nonatopic controls (n = 10), we investigated production by CD4+ T cells of tumour necrosis factor (TNF)-α, IL-4, IL-5, IL-13 and IL-10 in response to phorbol myristate acetate/ionomycin and Der p1 allergen. Results. It was observed that there were significantly higher frequencies of allergen-specific circulating CD4+ T cells producing TNF-α- IL-4-, IL-5- and IL-13, and lower frequencies of these
TY - JOUR. T1 - Characterization of the allergens of the house dust mite Euroglyphus maynei. AU - Morgan, Marjorie S.. AU - Arlian, Larry G.. AU - Barnes, Kathleen C.. AU - Fernandez-Caldas, Enrique. PY - 1997. Y1 - 1997. N2 - Background: The house dost mite Euroglyphus maynei inhabits homes in many parts of the world and is the source of many allergens. Objective: The purpose of this study was to biochemically and immunologically characterize the major allergens of E. maynei. Methods: Proteins in an extract of E. maynei were separated into 20 fractions by using preparative isoelectric focusing. Most proteins and allergens were contained in fractions 7 to 17 with pH of 4.8 to 8.0. The fractions were further characterized by nonreducing sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting with the serum of 16 individuals sensitive to E. maynei. Results: Molecular weights and isoelectric points were assigned to 47 IgE- binding proteins in an E. maynei extract, and the ...
Food allergy is a serious health concern among infants and young children. Although immunological mechanism of food allergy is well documented, the molecular mechanism(s) involved in food allergen sensitization have not been well characterized. Therefore, the present study analyzed the mesenteric lymph node (MLN) transcriptome profiles of BALB/c mice in response to three common food allergens. Microarray analysis identified a total of 1361, 533 and 488 differentially expressed genes in response to β-lactoglobulin (BLG) from cows milk, ovalbumin (OVA) from hens egg white and peanut agglutinin (PNA) sensitizations, respectively (p | 0.05). A total of 150 genes were commonly expressed in all antigen sensitized groups. The expression of seven representative genes from microarray experiment was validated by real-time RT-PCR. All allergens induced significant ear swelling and serum IgG1 concentrations, whereas IgE concentrations were increased in BLG- and PNA-treated mice (p | 0.05). Treatment with OVA and
Skin prick tests are regarded as means to determine sensitization and should be interpreted in the light of clinical history, clinical picture and results of testing for specific IgE. According to the American College of Allergy, Asthma and Immunology (ACAAI) [11], 10-50 allergen extracts are used for skin prick testing. The European guidelines propose a panel of 18 respiratory allergens of which, simultaneously, improved standardization is encouraged [12]. While skin prick testing in respiratory allergies is a reliable diagnostic tool, in food allergy more false positive results are seen on the one hand, while on the other hand over 95% of patients negative in skin prick tests with food do not present with immediate type symptoms [13]. The skin prick results should be compared to the positive control prick with histamine dihydrochloride 10 mg/ml [14]. The calculation of a histamine equivalent prick -index (HEP) area may be helpful, where the allergen prick size is correlated to the size of the ...
In an effort to tighten the belt, I registered on mygrocerydeals.com a few years ago. Its a website which aggregates grocery market or supermarket flyers. You enter a minimal amount of personal information, the most critical being your zip code, and youre then able to view all of the special deals and sales listed in local supermarket flyers, by food category or by store. At some point, I fell off the penny pinching wagon, life got too busy or something, and I stopped using that website. When I visited the website today, I remembered it offers a feature to flag common food allergens as well as a few restricted diets so that an additional symbol is included in your results; in the case of food allergens, youll see an exclamation point indicating products containing or possibly containing a major food allergen. Red exclamation point for foods containing the allergens you flag, and yellow for potentially containing ...
This test is done to check for allergies to specific allergens. Its especially useful in kids whove had life-threatening reactions to a certain allergen and for whom a skin-prick test would be too dangerous.
The present invention relates to the following [1] to [21].[1] A method for immunological measurement of a specific protein in a processed food with the use of an antibody against the protein, comprising adding animal tropomyosin having no antigen cross-reactivity with the specific protein to be measured to an assay solution upon measurement and then measuring the specific protein.[2] The method according to [1], which is a method for immunological measurement of a specific protein that is a food allergen protein in a processed food with the use of an antibody against the food allergen protein, comprising adding animal tropomyosin having no antigenic cross-reactivity with the specific protein to be measured to an assay solution upon measurement and then measuring the specific protein.[3] The method according to [1] or [2], comprising extracting a food allergen protein from a processed food using an extraction solution containing a surfactant and a reducing agent, reacting the extracted food ...
Allergen results of 0.10-0.34 kU/L are intended for specialist use as the clinical relevance is undetermined. Even though increasing ranges are reflective of increasing concentrations of allergen-specific IgE, these concentrations may not correlate with the degree of clinical response or skin testing results when challenged with a specific allergen. The correlation of allergy laboratory results with clinical history and in vivo reactivity to specific allergens is essential. A negative test may not rule out clinical allergy or even anaphylaxis. ...
Allergen results of 0.10-0.34 kU/L are intended for specialist use as the clinical relevance is undetermined. Even though increasing ranges are reflective of increasing concentrations of allergen-specific IgE, these concentrations may not correlate with the degree of clinical response or skin testing results when challenged with a specific allergen. The correlation of allergy laboratory results with clinical history and in vivo reactivity to specific allergens is essential. A negative test may not rule out clinical allergy or even anaphylaxis. ...
A comprehensive safety evaluation was conducted to assess the potential allergenicity of newly introduced proteins in genetically modified (GM) crops. We assessed the allergenicity of CP4 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in GM soybeans. This assessment was performed by IgE immunoblotting with soy-allergic childrens sera, amino acid sequence homology with known allergens, and the digestibility of CP4 EPSPS. No differences in IgE-antigen binding by immunoblotting were found between GM soy samples and the corresponding non-GM samples ...
Author Summary Parasitic nematode worms cause serious health problems in humans and other animals. They can induce allergic-type immune responses, which can be harmful but may at the same time protect against the infections. Allergens are proteins that trigger allergic reactions and these parasites produce a type that is confined to nematodes, the nematode polyprotein allergens (NPAs). These are synthesized as large precursor proteins comprising repeating units of similar amino acid sequence that are subsequently cleaved into multiple copies of the allergen protein. NPAs bind small lipids such as fatty acids and retinol (Vitamin A) and probably transport these sensitive and insoluble compounds between the tissues of the worms. Nematodes cannot synthesize these lipids, so NPAs may also be crucial for extracting nutrients from their hosts. They may also be involved in altering immune responses by controlling the lipids by which the immune and inflammatory cells communicate. We describe the molecular
RESULTS. The median patient age was 8.2 years (range, 5.7-11.3 years), and the median 1-second forced expiratory volume was 95% of the predicted value. The median absolute eosinophil count in the peripheral blood was 0.48 x 109 /L and the ratio of total serum immunoglobulin E to the age-adjusted upper limit of the normal range was 2.7. Atopy, as defined by at least one positive response to skin prick testing, was found in 170 (83.3%) of the 204 patients. House dust mites were the most commonly sensitised aero-allergen (n=167; 81.9%). Sensitisation to food allergens was found in 47 (23.0%) of the patients. The self-reported frequency of asthmatic attacks was associated with a positive response to skin prick testing with animal allergens (P for trend = 0.001), whereas spirometric indices correlated with the degree of atopy and the presence of in vivo cockroach-specific immunoglobulin E ...
Dust mites are the largest offender in house dust worldwide and are the single, most important allergen associated with asthma. Thriving in humid, dark and warm environments, they make their homes primarily in pillows, mattresses, carpets and upholstered furniture, burrowing themselves deep into the fabric. Our homes are inundated with these microscopic, insect-like creatures and their debris, creating a hazardous environment for the allergy sufferer.. It is virtually impossible to completely eliminate dust mites from your home, just as it is impossible to cure allergies. The key is allergen avoidance, which can significantly reduce patients symptoms, decrease medication requirements, and dramatically improve their quality of life. By encasing mattresses, pillows and box springs in zippered covers with a dust mite barrier, the mites are removed from their food source and ultimately die. There are also products available which can kill the dust mite in carpets or deactivate mite protein in the ...
Kulkarni and colleagues first evaluated food and aeroallergen sensitization in the participants through history, skin prick tests for common aeroallergens and food substances, and, in the food allergy group, through the presence of IgE in serum. The results showed that all of the subjects with food allergies were additionally sensitized to at least one aeroallergen, and sensitization to house dust mites was the highest, followed by pollen, pet dander, and fungi. Sensitization to dust mites was also elevated in asthmatic children without food allergies. No statistically significant differences were found between aeroallergen sensitization in asthmatic patients with or without food allergies. The authors then measured exhaled nitric oxide through FeNO analysis, a surrogate marker of eosinophilic inflammation, and found that children with both asthma and food allergies had significantly higher FeNO median than asthmatic children without food allergy. Next, the authors determined eosinophil counts ...
Cross-Network collaborations among AllerGens Enabling Platform teams have resulted in translational research results in food allergy, epigenetics and biomarker development that facilitated the development of new approaches to allergy diagnosis and clinical care. These collaborations have also supported the launch of The Canadian Urban Environmental Health Research Consortium (CANUE)-a new pan-Canadian research consortium that links exposome data with AllerGen research and CHILD Study data to advance our understanding of how cities can be designed or modified to improve population health.. In 2016-2017, AllerGen teams joined with global partners to provide novel capacity building opportunities to outstanding young trainees and investigators, accelerating their rise through the academic ranks. AllerGen was also delighted to award its fourth Emerging Clinician-Scientist Research Fellowship, valued at $250,000, to support the development of Canadian clinician-scientists in the field of allergy and ...
Contrary to previous advice, new EU rules relating to allergens labelling will apply to all wine labelled after 30 June 2012.. Wine Australia had previously been informed that 2012 and earlier vintage dated wine would be exempt from the labelling requirement regardless of when labelled, but that is no longer the EU position. Consequently, wine arriving in the EU after 30 June will only be exempt from the requirement to declare the presence of allergens if it was labelled before 30 June 2012.. For wines fined with allergenic substances, it will not be necessary to declare the presence of egg and milk allergens on the label provided the wine has tested negative for the presence of these residual allergens using a technique with a detection limit of 0.25 mg/L (ppm).. The EU is yet to publish a list indicating the languages accepted in each of the 27 member states. Wine Australias Export Market Guide to the EU (http://www.wineaustralia.com) includes a list of acceptable languages for the contains ...
The Journal of Investigational Allergology and Clinical Immunology (J Investig Allergol Clin Immunol) provides an attractive and very active forum for basic and clinical research in allergology and clinical immunology. The journal publishes original works, reviews, short communications and opinions. It is the Official Journal ofthe Spanish Society of Allergology and Clinical Immunology (SEAIC). The journal will appear bimonthly (6 issues each year). The peer-review system, based on a team of internationally renowned and committed editors, guarantees the scientific quality of the papers.. ...
Families across the country have plenty to think about during back-to-school season. For parents, new school supplies and changing schedules are top of mind, while kids are thinking about new friends, classes and teachers. But for families of children with food allergies, returning to school can be even more stressful as they might worry about accidental exposure and potential anaphylaxis.. Food allergies are increasingly common in American schools. According to Food Allergy Resources and Education (FARE), one in 13 children have at least one food allergy, and more than 15% of school-aged children with a food allergy have had a reaction while at school. Some schools are taking steps to create safer environments for students with food allergies - like establishing nut-free classroom policies - and researchers are studying new oral immunotherapy (OIT) candidates as an option to help desensitize children to their allergen. OIT involves providing controlled doses of an allergen to a child with the ...
Caesars Pasta, LLC Recalls Beef Meatball Products Due To Misbranding and Undeclared Allergens Caesars Pasta, LLC, a Blackwood, N.J. establishment, is recalling approximately 46,810 pounds of beef meatball products due to misbranding and undeclared allergens. The products contain egg, a known allergen, which is not declared on the product label. ...
To determine the effect of humidity on the levels of the mouse allergen Mus m 1, an experimental animal room was constructed to control environmental variables. The sex, strain, age, and number of mice was constant in the room, so that the average daily production of Mus m 1 would not vary greatly. Six different levels of relative humidity from 15% to 65% were maintained for a minimum of a week each. Daily collections of airborne particulates were eluted from filters and Mus m 1 content measured by immunological assay. Increasing relative humidity caused a decrease in Mus m 1 levels from a high of 3 ng/m3 at 15% humidity to a low of 0.5 ng/m3 at 65% humidity. Thus, reduction of airborne allergen levels can be achieved by careful attention to humidity control, especially during the winter heating season when humidity levels may be low. This experimental room can be used to measure the effect of other variables such as ventilation rate, caging, bedding, and work practices on the levels of
Dust builds up throughout your home. The dust may contain substances that trigger asthma symptoms, such as wheezing or coughing, or another allergic reaction, such as the rash of atopic dermatitis or stuffy nose of allergic rhinitis. These substances are called allergens. Dust mites are another example of an allergen...
TY - JOUR. T1 - Allergenic characterization of 27-kDa glycoprotein, a novel heat stable allergen, from the pupa of silkworm, Bombyx mori. AU - Jeong, Kyoung Yong. AU - Son, Mina. AU - Lee, June Yong. AU - Park, Kyung Hee. AU - Lee, Jae Hyun. AU - Park, Jung Won. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Boiled silkworm pupa is a traditional food in Asia, and patients with silkworm pupa food allergy are common in these regions. Still now only one allergen from silkworm, arginine kinase, has been identified. The purpose of this study was to identify novel food allergens in silkworm pupa by analyzing a protein extract after heat treatment. Heat treated extracts were examined by proteomic analysis. A 27-kDa glycoprotein was identified, expressed in Escherichia coli, and purified. IgE reactivity of the recombinant protein was investigated by ELISA. High molecular weight proteins (above 100 kDa) elicited increased IgE binding after heat treatment compared to that before heat treatment. The molecular ...
What Allergies Can Pets Have? The most common types of allergies in pets are flea allergy, food allergy, and a condition called atopy. Atopy is sometimes called atopic dermatitis or allergic inhalant dermatitis, and it occurs when allergens that are inhaled or that contact the skin cause an allergic reaction in the body. In dogs (and, less commonly, cats), this allergic reaction is focused largely in the skin. Animals with atopy become very itchy; the resultant scratching can lead to skin injuries and subsequent skin infections. Atopy is usually first noticed in dogs younger than 3 years of age, although older pets can also be affected. Unfortunately, some pets that develop atopy continue to have problems throughout their lives. Many types of allergens can cause a pet to develop atopy. A wide variety of pollens, grasses, dander, insect proteins (such as in cockroaches), molds, and even house dust can cause animals to develop atopy. Animals can even develop allergies to multiple allergens at the ...
Learn how to reduce airborne allergies & allergens from the Cleveland Clinic, including tips on how to reduce animal allergens & other allergens.
TY - JOUR. T1 - Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation. AU - Zosky, G. R.. AU - Larcombe, A. N.. AU - White, O. J.. AU - Burchell, J. T.. AU - Janosi, T. Z.. AU - Hantos, Z.. AU - Holt, P. G.. AU - Sly, P. D.. AU - Turner, D. J.. PY - 2008/5. Y1 - 2008/5. N2 - Background: Asthma is a chronic inflammatory disease that is characterized clinically by airway hyperresponsiveness (AHR) to bronchoconstricting agents. The physiological response of the asthmatic lung to inhaled allergen is often characterized by two distinct phases: an early-phase response (EPR) within the first hour following exposure that subsides and a late-phase response (LPR) that is more prolonged and may occur several hours later. Mouse models of asthma have become increasingly popular and should be designed to exhibit an EPR, LPR and AHR. Objective: To determine whether a common model of asthma is capable of demonstrating an EPR, LPR ...
According to the Centers for Disease Control and Prevention, more than 8% of children in the United States currently have asthma, a chronic disease that intermittently inflames and narrows the airways. Asthma can result in missed time from school and work and is a major cause of emergency department visits and hospitalizations. According to an article published on line in the Journal of Allergy and Clinical Immunology (19 September 2017), children exposed to high indoor levels of pet or pest allergens during infancy have a lower risk of developing asthma by 7 years of age. While previous studies have established that reducing allergen exposure in the home helps control established asthma, the new findings suggest that exposure to certain allergens early in life, before asthma develops, may have a preventive effect. The observations come from the ongoing Urban Environment and Childhood Asthma (URECA-pronounced Eureka ) study, which is funded by NIAID through its Inner-City Asthma ...