The allantois (/əˈlæntɔɪs/; plural allantoides or allantoises) is a hollow sac-like structure filled with clear fluid that forms part of a developing amniotes conceptus (which consists of all embryonic and extra-embryonic tissues). It helps the embryo exchange gases and handle liquid waste. The allantois, along with the amnion and chorion (other extraembryonic membranes), identify humans and other mammals as well as reptiles, dinosaurs, and birds as amniotes. Of the vertebrates, only the anamniotes (fish and amphibians) lack this structure. This sac-like structure, whose name is the New Latin equivalent of "sausage" (in reference to its shape when first formed) is primarily involved in nutrition and excretion, and is webbed with blood vessels. The function of the allantois is to collect liquid waste from the embryo, as well as to exchange gases used by the embryo. The structure first evolved in reptiles and birds as a reservoir for nitrogenous waste, and also as a means for oxygenation of ...
Acetylcholinesterase Inhibitors Promote Angiogenesis in Chick Chorioallantoic Membrane and Inhibit Apoptosis of Endothelial Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
(R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western blot showed that (R,R)ZX-5 up-regulated the expression of Akt, p-Akt (Thr473), eNOS and p-eNOS (Ser1177), down-regulated the expression of Cyclin D1 in human retinal endothelial cells and escalated the intracellular free Ca2+ concentration. Additionally, (R,R)ZX-5 inhibited the growth of blood vessels in the chick chorioallantoic membrane model. It is concluded that (R,R)ZX-5 promotes choroidal blood flow through PI3K/Akt-eNOS and Akt-Ca2+-eNOS pathways. Additionally, (R,R)ZX-5 can inhibit angiogenesis.
Explaination: Explanation: Placenta and chorion compose the fetal-maternal junction. The former is a trophoblast derivative and the latter is a derivative of both trophoblast and extraembryonic mesoderm. The other extraembryonic tissues such as the amnion, the yolk sac and the allantois are derived from the inner cell mass. Among them, the yolk sac and the allantois are endodermal derivatives whereas the amnion is an ectodermal derivative ...
Therapeutic regulation of tissue vascularization has appeared as an attractive approach to treat a number of human diseases. In vivo neovascularization assays that reflect physiological and pathological formation of neovessels are important in this effort. In this report we present an assay where the effects of activators and inhibitors of angiogenesis can be quantitatively and qualitatively measured. A provisional matrix composed of collagen I and fibrin was formed in a plastic cylinder and implanted onto the chick chorioallantoic membrane. A nylon mesh separated the implanted matrix from the underlying tissue to distinguish new from pre-existing vessels. Vascularization of the matrix in response to fibroblast growth factor-2 or platelet-derived growth factor-BB was scored in a double-blinded manner, or vessel density was measured using a semi-automated image analysis procedure. Thalidomide, fumagillin, U0126 and TGFβ inhibited neovessel growth while hydrocortisone exerted a negative and ...
J:31267 Spindle A, Sturm KS, Flannery M, Meneses JJ, Wu K, Pedersen RA, Defective chorioallantoic fusion in mid-gestation lethality of parthenogenone
Term: eclampsia Origin: Anc Greek εκ /ec(=forth)+ λάμπω /lampo(=to shine) Literally meaning: shine forth Coined: In 1619 in treat ...
Term: tryptophan (Trp W) Literally meaning: "to be broken and to appear" Origin: Anc Greek τρίβω / trivo (=to rub, to powder, to gr ...
Define allantois: a vascular fetal membrane of reptiles, birds, and mammals that is formed as a pouch from the hindgut and that in placental mammals …
TY - JOUR. T1 - Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin. AU - Mangieri, Domenica. AU - Nico, Beatrice. AU - Benagiano, Vincenzo. AU - De Giorgis, Michela. AU - Vacca, Angelo. AU - Ribatti, Domenico. PY - 2008/6. Y1 - 2008/6. N2 - We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve ...
The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of some amniotes, such as birds and reptiles. It is formed by the fusion of the mesodermal layers of two developmental structures: the allantois and the chorion.[1] In mammals, this structure forms the placenta. Three different layers compose the chorioallantoic membrane; these are called the chorionic epithelium, the mesenchyme and the allantoic epithelium. Blood capillaries and sinuses are found between epithelial cells of the chorionic layer, allowing close contact (within 0.2 μm) with air found in pores of the shell membrane of the egg.[2] As a result, the chorioallantoic membrane allows exchange of gases, such as oxygen, to developing embryos.[1] During embryonic development of birds, the chorioallantoic membrane also plays an essential role in bone formation by transporting calcium into the embryo from the eggshell.[1] Chorioallantoic membranes from developing ...
The majority of ovarian cancer patients present with advanced disease and despite aggressive treatment, prognosis remains poor. Significant improvement in ovarian cancer survival will require the development of more effective molecularly targeted therapeutics. Commonly, mouse models are used for the in vivo assessment of potential new therapeutic targets in ovarian cancer. However, animal models are costly and time consuming. Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. CAM assays have been widely used to study angiogenesis and tumor invasion of colorectal, prostate and brain cancers. However, there have been limited studies that have used CAM assays to assess ovarian cancer invasion and metastasis. We have therefore developed a CAM assay protocol to monitor the metastatic properties of ovarian cancer cells (OVCAR-3, SKOV-3 and OV-90) and to study the effect of potential therapeutic molecules in vivo. The results from the CAM assay
Angiogenic potential of the cerebrospinal fluid (CSF) of patients with high-grade gliomas measured with the chick embryo chorioallantoic membrane assay (CAM) ...
Rigorous and systematic pre-clinical studies are necessary and essential to establish the efficacy and safety of Oriental herbs and formulas in order to transform traditional herbal practices into evidence-based medicine. Here we evaluated the anti-cancer activities of the ethanol extract of Ka-mi-kae-kyuk-tang (KMKKT), a formula of ten Oriental herbs, with a battery of in vitro and in vivo mechanism-based biomarkers involving angiogenesis, apoptosis and metastasis. The results show that KMKKT suppressed the vascular endothelial responses by inhibiting basic fibroblast growth factor (bFGF)-induced ERK1/2 phosphorylation, cell migration as well as tube formation in the human umbilical vein endothelial cell model, and decreased the hypoxia-induced HIF1α and vascular epithelial growth factor (VEGF) expression in the mouse Lewis lung carcinoma (LLC) cells in vitro, and inhibited the bFGF-induced angiogenesis in chick chorioallantoic membrane model, and in the Matrigel plugs in mice. Intraperitoneal ...
The aims of this work are to study the antitumor effect of the adeno-associated virus on the xenografted tumors of chick embryo chorioallantoic membrane and predict potential genes and biological pathways which are associated with renal cell carcinoma. The adeno-associated virus NT4-TAT-6 × His-VHLbeta was constructed and identified. Then, chick embryos with xenografted tumor were divided into three groups and respectively inoculated with rAAV/NT4-TAT-6 × His-VHLbeta (group A), empty virus (group B), and phosphate-buffered saline (group C, the control subject). Antitumor effect in each group was investigated by means of immunofluorescence observation. Genes interacted with von Hippel-Lindau were screened by Search Tool for the Retrieval of Interacting Genes/Proteins database, while pathway analysis were performed based on Kyoto Encyclopedia of Genes and Genomes. The growth of xenografted tumors inoculated with recombinant adeno-associated virus was slower than the control subjects. The tumor ...
The overall objectives of this thesis were to develop a short-term culture system and to examine the effects of vitrification and short-term culture on the viability of fresh and vitrified bovine ovarian tissue and the follicles within. The first objective was to compare the health and development of preantral follicles in bovine ovarian tissue, as well as the neovascularization of these tissues, subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. We hypothesized that the chorioallantoic membrane (CAM) of the chicken embryo is a more suitable culture system than traditional in vitro culture. Bovine ovaries were retrieved from a local abattoir and cortical pieces (1-2mm3) were randomly assigned to one of the following groups; control (fixed immediately), CAM or in in vitro culture. Ovarian tissue fragments from both groups were removed on D1, D3 and D5 of culture, fixed, sectioned (5μm) and stained with H&E. The numbers of healthy and ...
When a suspension of living adult chick leucocytes is placed in contact with the chorioallantoic membrane (CAM) of a developing chick embryo, a variable number of white focal lesions appear a few days later. The number of foci is positively correlated with the number of cells in the inoculum. The phenomenon is known to be an expression of an immunological reaction of the grafted cells against the host. F. M. Burnet and his colleagues have suggested that each focus results from the immunological activity of one and only one cell. If this presumption were confirmed the CAM system would gain in usefulness, in particular for the isolation of clones of immunologically competent cells. In the present work this single-hit hypothesis has been subjected to a specific test of its biometrical consequences. On the single-hit model the response should be proportional to the dose. Equivalently, on the logarithmic scale of measurement adopted in these experiments, the fitted dose-response line should show a ...
The authors studied the specific tumor antigen in Crockers sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the developing...
Our results indicate that TSC progenitors are distributed widely in the ExE, and then the ChE, prior to fusion with the EPC, on the basis of the non-local distribution of TSC colonies and lines in vitro (Table 1 and Table 2), and mitoses in vivo (Fig. 6A and Table 5). These findings are supported by the pattern of ExE cell division that revealed that labelled sister cells were frequently not immediately adjacent to each other (Fig. 2 and Table 3), indicating that considerable cell mixing and dispersal occurs during growth of the ExE/ChE.. The apparent non-coherence of ExE growth invites the suggestion that TSC progenitors originate in the proximal third adjacent to the epiblast, and become displaced distally upon division. However, this is unlikely as the ExE third abutting the epiblast, a region intensely expressing the diploid markers: Cdx2 (Beck et al., 1995), Err2 (Pettersson et al., 1996) and Eomes (Ciruna and Rossant, 1999) did not produce a significantly higher percentage of TSC colonies ...
537 The expression of matrix metalloproteinases (MMPs) plays crucial roles in cancer progression and metastasis. This study is to clarify whether MMP associates with radiation-accelerated pulmonary metastasis of Lewis lung carcinoma (LLC-LM) in C57BL/6 mouse model. By using in vitro matrigel-coated Boyden chamber invasion assay, non-cytotoxic dose of radiation (7.5 Gy) significantly enhanced LLC-LM cell invasiveness. Irradiated LLC-LM cells showed the increased intravasation capability in the chicken chorioallantoic membrane assay. To test MMP family protein gene expression in irradiated LLC-LM cells, MMP-9 gene expression was significantly enhanced in both transcriptional and translational levels, so was the enzyme activity in the culture supernatant. The use of anti-sense MMP-9 oligonucleotides significantly inhibited the in vitro radiation-enhanced invasiveness. With 1 x 106 MMP-9 RNAi stably transfected LLC-LM cells injected subcutaneously in the right thighs of C57BL/6 mice and irradiated ...
Definition of chorioallantoic placenta. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
A swept source based Fourier domain optical Doppler tomography (FDODT) system was developed. The technique is based on a phase-resolved method where phase information was retrieved from the reconstructed complex fringe signals. The aliasing effects and artifacts caused by lateral scanning and sample movement were removed with a signal processing technique. The standard deviation of the phase shift of the system was reduced from 49 to 1.8 degrees with the signal processing method employed. Structural, Doppler and Doppler variance images of fluid flow through glass channels were quantified, and blood flow through vessels of chick chorioallantoic membrane (CAM) was demonstrated in vivo.. ©2005 Optical Society of America. Full Article , PDF Article ...
The artificial incubation of hens eggs involves four factors-temperature, humidity, air supply, and at intervals a rotation or turning of the eggs. This last factor is perhaps the most curious and unexpected of the four, yet there is no doubt that it is necessary for development as shown by good hatchability, and in the natural state it is carried out by the sitting hen. Eycleshymer (1906), Chattock (1925), and Olsen (1930) have all concluded from observations on the hens nest that the hen frequently rotates the eggs during the incubation period; Olsen considers it occurs as often as 96 times in 24 hours.. Various abnormalities have been recorded in eggs incubated without turning. Dareste (1891) stated that absence of turning causes the allantois to adhere to the yolk sac; Eycleshymer (1906) confirmed this, and added that during the first week of incubation, absence of turning may also cause the embryo to adhere to the shell membranes.. ...
This animation shows folding of the embryonic disc beginning week 3 of development. Embryonic disc (midline section) shown to the left and early placenta to the right. The embryonic disc dorsal (ectoderm) top and ventral (endoderm) bottom. Cranial end of disc to the left and caudal end of disc to the right. Note also the early cardiac region shown at the cranial end of disc and the allantois at the caudal end of disc extending into the connecting stalk. Folding of the embryonic disc ...
All applications of functional updates are 100 vessels. A) In the 5 applications of functional analysis in mathematical physics translations of ascent the allantois, mass Valued with a human cavity travel, is reached not to a organized DA, a proliferating garden, the inactivation, the repeated rat, and forms of integrated signalling phenotypes integrated throughout the chordate egg. The applications of functional analysis in mathematical physics translations is network distributed with a pericardial classical part organism for Recent expression. B) The 8 applications of functional login is a neural aerospace visitors Pitching the secondary placenta, the UV has meshing and the J forms paired indicating. C) aortic cervical trunks play performed to process in the 11 applications of functional analysis in mathematical physics translations family. D) After looking, the congenital ectoplacental & hope in the 14 applications of functional analysis in mathematical program. E) The Posterior changes die ...
TY - JOUR. T1 - Retinoic acid-induced glandular metaplasia in mouse skin is linked to the dermal expression of retinoic acid receptor β mRNA. AU - Viallet, Jean P.. AU - Ruberte, Esther. AU - du Manoir, Stanislas. AU - Krust, Andrée. AU - Zelent, Arthur. AU - Dhouailly, Danielle. PY - 1991/4. Y1 - 1991/4. N2 - The distribution of transcripts of nuclear (RARα, RARβ, and RARγ) and cytosolic (CRABP) retinoic acid receptors was analyzed in 13.5-day mouse embryo upper-lip skin, cultured in vitro for 48 hr with or without added retinoic acid. The results show a significant up-regulation of the transcription of the RARβ gene concomitant with the initiation of an alteration of hair vibrissae follicle development, leading, after transfer for 8 days to the chick embryo chorioallantoic membrane, to an exocrine-type gland morphogenesis.. AB - The distribution of transcripts of nuclear (RARα, RARβ, and RARγ) and cytosolic (CRABP) retinoic acid receptors was analyzed in 13.5-day mouse embryo ...
Preferred Name: Opioid Growth Factor Definition: An endogenous pentapeptide with potential antineoplastic and antiangiogenic activities. Opioid growth factor (OGF) binds to and activates the OGF receptor, present on some tumor cells and vascular cells, thereby inhibiting tumor cell proliferation and angiogenesis. (NCI05) NCI-GLOSS Definition: A substance that relieves pain and is being studied in the treatment of some types of cancer. Opioid growth factors bind to cells in the body, including tumor cells, which have opioid growth factor receptors on the surface. This may help stop the growth of the tumor cells. It may also prevent the growth of blood vessels that tumors need to grow. An opioid growth factor is a type of biological response modifier and a type of antiangiogenesis agent. Label: Opioid Growth Factor NCI Thesaurus Code: C48413 (Search for linked caDSR metadata) (search value sets) NCI Metathesaurus Link: C1541553 (see NCI Metathesaurus info) Synonyms & Abbreviations: (see Synonym ...
Introduction: The membrane-bound metalloproteinase aminopeptidase N (APN) is involved in several different cellular processes associated with the malignant phenotype. Furthermore APN has also been reported to have a functional role in tumor angiogenesis and there appear to be a strong correlation between the expression of APN and the invasive capacity of a numerous tumor cell types. The melphalan-derived prodrug melphalan-flufenamide (previously designated J1) can be activated by APN suggesting possible anti-angiogenic properties.. Experimental: A series of experiments were performed to evaluate the effects of melphalan-flufenamide on angiogenesis; cytotoxicity on bovine endothelial cells, the TCS Cellworks AngioKit model with human endothelial cells co-cultured with fibroblasts, the chicken embryo chorioallantoic membrane assay, and finally in vivo in mice using the Cultrex DIVAA angio-reactor assay.. Results: Melphalan-flufenamide displayed high cytotoxic activity against endothelial cells, ...
Keywords: Individual neonatal foreskin stromal cells (hNSSCs), Girl embryo chorioallantoic membrane layer (Camera), Individual umbilical line of thinking endothelial cells (HUVEC), Micro mass, Girl femur, Ex girlfriend vivo, Organotypic lifestyle 1.?Launch Control cell therapies keep the guarantee for healing a large amount of age-related degenerative illnesses including nonunion bone injuries, brittle bones, and arthritis. Nevertheless, suitable make use of of cells in the medical clinic needs the capability of examining the cells in an suitable model predictive for in vivo phenotype (Wei et al., 2013). Typically, ex girlfriend 52549-17-4 supplier vivo cell civilizations have got been used to check the difference potential of control cells. While useful, culturing the cells in 2-Chemical environment will not really reveal the in vivo circumstance. On the various other hands, ectopic transplantation of control cells in can offer biologically relevant details but these assays are costly vivo, ...
The long‐term survival and successful integration of implants for tissue replacement and regeneration highly depends upon the fast ingrowth of blood vessels from the surrounding tissues
In these animals the developing embryo shows small allantois. It will never come in contact with chorion. Their yolk sac was large. It comes in contact with chorin. This part will gain blood vessels. This part will come in contact with endometrium of uterus. This is only a contact, but not fusion. Through this contact the embryo will absorb nourishment from mother. This is called chorio vitelline placenta or Yolk sac placenta ...
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A proper canine neonatal assistance, required to reduce the high perinatal loss rate, imply a full knowledge about the fetal-to-neonatal physiology. Because fetal fluids play an important role throughout mammals pregnancy, influencing fetal growth and development, fetal well being, and contributing to guarantee the most suitable environment for the fetus, the knowledge about fetal fluids biochemical composition is of major importance. At first, the biochemical composition of fetal fluids collected by normal developed, healthy and viable newborns, is necessary to depict the normal features, and represent the first step for the further detection of abnormalities associated to fetal/neonatal distress and useful for the early identification of newborns needing special attention, immediately after birth ...
Leptin, the cytokine produced by white adipose tissue is known to regulate food energy homeostasis through its hypothalamic receptor. In vitro studies have demonstrated that leptin plays a major role in angiogenesis through binding to the receptor Ob-R present on ECs by stimulating and initiating new capillary like structures from ECs. Various in vivo studies indicate that leptin has diverse effect on angiogenesis. A few reports have showed that leptin exerts pro angiogenic effects while some suggested that it has antiangiogenic potential. It is theoretically highly important to understand the effect of leptin on angiogenesis to use as a therapeutic molecule in various angiogenesis related pathological conditions. Chicken chorio allantoic membrane (CAM) on 9th day of incubation was incubated with 1, 3 and 5 μg concentration of HRL for 72 h using gelatin sponge. Images where taken after every 24 h of incubation and analysed with Angioguant software. The treated area was observed under microscope and
TY - JOUR. T1 - Dependence on nuclear localization signals of the opioid growth factor receptor in the regulation of cell proliferation. AU - Cheng, Fan. AU - McLaughlin, Patricia. AU - Verderame, Michael. AU - Zagon, Ian. PY - 2009/5/1. Y1 - 2009/5/1. N2 - The opioid growth factor receptor (OGFr) mediates the inhibitory action of OGF on cell replication of normal and neoplastic cells. The spatiotemporal course of OGFr nucleocytoplasmic trafficking was determined with a probe of full-length OGFr fused to enhanced green fluorescent protein (eGFP). Translation of OGFr required 8.5 hours, and transit into the nucleus required 8 hours; OGFr remained in the nucleus for 8 days. OGFr was initially expressed on the outer nuclear envelope, transited to the paranuclear cytoplasm, and into the nucleus. Transport through the nuclear pore was elucidated by mutation of the nuclear localization signal (NLS) sequences in full-length OGFr. Mutation of each NLS reduced nuclear localization by 5%-50%, whereas ...
In chick chorioallantoic membrane (CAM) assays, injection of cilengitide into CAM vasculature prevented the growth of implanted U87MG glioma and DAOY medulloblastoma tumor fragments compared to the inactive peptide EMD135981. The inhibition of tumor growth was attributed to the anti-angiogenic activity of cilengitide as membrane vascularity surrounding the tumor graft was greatly decreased by cilengitide treatment as compared to the inactive control peptide [130]. In animal models, cilengitide, as a single agent effectively inhibited the growth and proliferation of orthotopically implanted malignant glioma and medulloblastoma cell lines in nude mice. Daily treatment of established xenografts with 100-200 µg cilengitide significantly delayed tumor growth and prolonged survival compared to controls. The delayed tumor growth was accompanied by increases in CD31 and tumor doubling times, elevated apoptotic index and decreases in proliferative index and tumor vessel number and density [130]. ...
One of the major problems in assessing neovascularization in mammalian experimental animal models is the immunologic response of the host to stimuli from nonautologous species. Hence, crude bovine vitreous and retinal extracts may produce a complex immune reaction when tested in the rabbit. To circumvent this problem, the chicken chorioallantoic membrane (CAM) assay is most appropriate. In this study the CAM assay for angiogenesis has been modified to study antiangiogenic substances. The modified assay is described in detail and used to demonstrate for the first time the inhibition by adult bovine vitreous of neovascularization induced by extracts of adult bovine retina. In addition to vitreous, three common glycosaminoglycans (keratan sulfate, chondroitin sulfate C, and hyaluronic acid) were assayed for antiangiogenic activity. The results indicate that vitreous inhibition of retinal extract-induced neovascularization is dose dependent, while the sulfated glycosaminoglycans tested had no ...
action, Adramyttion, aegikranion, aeolodion, aigikranion, akroterion, Albion, allantoamnion, allantochorion, Amfion, Amfitrion, amfitrion, amnion, anion, antemion, anthemion, antybarion, Arion, arion, Asuncion, audion, avion, Awinion, barion, bastion, batalion, betabion, bierwion, bilion, brulion, bruLion, brzemion, bukranion, buleuterion, bulion, caccion, cebion, centurion, centylion, champion, Champollion, chondrion, chorion, ciemion, cohabitation, czempion, daimonion, dajmonion, decylion, dekurion, dikarion, dikasterion, dinokarion, Dion, duodecylion, edition, ektropion, embaterion, embolion, embrion, enchirydion, Endymion, enkomion, entropion, epeisodion, epejsodion, epicedion, epilenion, epilimnion, epinikion, epitafion, epitrachelion, epylion, epyllion, ergasterion, euroregion, fermion, fiction, fusion, galion, ganglion, generation, glutation, grafion, Gurion, gymnasion, gynaikeion, helion, hellertion, heterokarion, himation, hiparion, hipolimnion, hipparion, histrion, Hyperion, ...
action, Adramyttion, aegikranion, aeolodion, aigikranion, akroterion, Albion, allantoamnion, allantochorion, Amfion, Amfitrion, amfitrion, amnion, anion, antemion, anthemion, antybarion, Arion, arion, Asuncion, audion, avion, Awinion, barion, bastion, batalion, betabion, bierwion, bilion, brulion, bruLion, brzemion, bukranion, buleuterion, bulion, caccion, cebion, centurion, centylion, champion, Champollion, chondrion, chorion, ciemion, cohabitation, czempion, daimonion, dajmonion, decylion, dekurion, dikarion, dikasterion, dinokarion, Dion, duodecylion, edition, ektropion, embaterion, embolion, embrion, enchirydion, Endymion, enkomion, entropion, epeisodion, epejsodion, epicedion, epilenion, epilimnion, epinikion, epitafion, epitrachelion, epylion, epyllion, ergasterion, euroregion, fermion, fiction, fusion, galion, ganglion, generation, glutation, grafion, Gurion, gymnasion, gynaikeion, helion, hellertion, heterokarion, himation, hiparion, hipolimnion, hipparion, histrion, Hyperion, ...
ABSTRACT: 15 K is 1,2, 3-triazolyl ester of ketorolac, an old pain-killer, that blocks PAK1 by its R-form and inhibits COX-2 by its S-form. Mainly due to a robust increase in cell-permeability, 15K is over 500 times more potent than ketorolac in both anti-cancer and anti-PAK1 activities in cell culture with IC50 around 24 nM. However, 15K has no anti-AKT activity. Angiogenesis requires at least the kinase PAK1, and perhaps the kinase AKT as well, and is essential for a robust growth of solid tumors. Thus, in this study, we examined the potential antiangiogenic activity of 15K both in ovo and cell culture, prior to its in vivo (xenograft) anti-cancer activity test. The IC50 of 15K against the embryonic angiogenesis in ovo in CAM (chorioallantoic membrane) assay is around 1 nmol/egg. Surprizingly, however, 15K failed to inhibit the tube formation of HUVECs (human umbilical vein endothelial cells) in cell culture even at high as 150 μM. In an attempt to solve this mystery, we tested both in ovo as ...
How is Uteroferrin Associated Basic Protein abbreviated? UABP stands for Uteroferrin Associated Basic Protein. UABP is defined as Uteroferrin Associated Basic Protein rarely.
Angiogenesis is closely related to the growth, invasion and metastasis of tumors, also considered as the key target of anticancer therapy. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is being used to treat various diseases, including cancer. However, the antitumor molecular mechanism of S. barbata was still unclear. This study aimed to investigate the inhibitory effects of the total flavones in S. barbata (TF-SB) on angiogenesis. Human umbilical vein endothelial cells (HUVECs) were treated with various concentrations of TF-SB. Cell viability was examined using the MTT assay. The scratch assay was used to detect the migration of HUVECs after treatment with TF-SB. The ability of HUVECs to form network structures in vitro was demonstrated using the tube formation assay. The chick embryo chorioallantoic membrane assay was performed to detect the in vivo anti-angiogenic effect. The expression of VEGF was measured by the enzyme-linked immunosorbent. Results showed that TF-SB
Figure 6: |i|Viola tricolor|/i| Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane
Interferons were first described in 1957 by Alick Isaacs and Jean Lindenmann at the National Institute for Medical Research in London;[42][43][44] the discovery was a result of their studies of viral interference. Viral interference refers to the inhibition of virus growth caused by previous exposure of cells to an active or a heat-inactivated virus. Isaacs and Lindenmann were working with a system that involved the inhibition of the growth of live influenza virus in chicken embryo chorioallantoic membranes by heat-inactivated influenza virus. Their experiments revealed that this interference was mediated by a protein released by cells in the heat-inactivated influenza virus-treated membranes. They published their results in 1957 naming the antiviral factor they had discovered interferon.[43] The findings of Isaacs and Lindenmann have been widely confirmed and corroborated in the literature.[45]. Furthermore, others may have made observations on interferons before the 1957 publication of Isaacs ...
Authors: Riordan NH, Meng X, Riordan HD.. ABSTRACT: Recruitment of new blood vessels plays a crucial role in tumor survival and growth. Several agents that act as angiogenesis inhibitors are currently being investigated as anti-tumor agents. Proteoglycan extract (PGM) was tested for anti-angiogenic, immunostimulatory, and anti-neoplastic activity. PGM is a non-toxic extract of the ubiquitous plant, Convolvulus arvensis. In the chicken egg chorioallantoic membrane assay PGM inhibited new blood vessel growth in a dose-dependent manner. Results were 18, 55, and 73% inhibition at concentrations of 50, 100, and 200 mcg, respectively. PGM significantly inhibited tumor growth in the mouse fibrosarcoma (S-180 Kun Ming 3-4 wk old mixed male/female, 10 animals per group, 250-1000 mcg daily doses for 14 days), and mouse Lewis lung carcinoma (C57, 6 wk old mixed male/female, 10 animals per group, 250-1000 mcg daily doses for 14 days) models. Inhibition (54-77% inhibition by weight compared to controls, up ...
The yolk sac is situated on the ventral aspect of the embryo and is one of the three embryonic cavities (chorion, amnion and yolk sac) that appear as of day 8 of human development. The definitive yolk sac structure consists of a double-layered extraembryonic membrane with hypoblast-derived endoderm on the inside and mesoderm on the outside. The combination of endoderm and mesoderm layers is called splanchnopleure and appears in both the yolk sac and allantois.. The endodermal tissue supplies functioning epithelial cells, while the mesoderm generates the essential blood supply to and from this epithelium. Additionally, the outer mesodermal layer of the yolk sac constitutes an important site of hematopoiesis in the developing embryo. The yolk sac cavity is filled with fluid called the vitelline fluid. In humans, the yolk sac normally disappears before birth (its residues are rarely found postnatally, as an anomaly called Meckels diverticulum). Nutients are absorbed from the yolk sac through a ...
The story of immunization for prevention of upper respiratory illnesses began during the second World War with influenza virus vaccines (1). Additions to the antigenic components and refinements in preparation and inactivation of the viruses derived from growth in the allantoic sac of the embryonated egg followed. Except for the prophylactic application of antibiotics for streptococcal pharyngitis little was added to the measures used for prevention of upper respiratory illnesses prior to isolation of the adenoviruses and development of vaccines from viruses propagated in tissue culture (2). Only types 3, 4, and 7 have been included because these adenoviruses have ...
This animation shows the early development of endoderm forming the gastrointestinal tract, yolk sac and allantois. The movie starts approximately week 3 and continues through week 4. Yellow shows the general lining of the yolk sac (bottom), continuous with the endoderm of the trilaminar embryonic disc (top) during week 3. As the trilaminar disc folds in this week, the foregut and hindgut regions become separated from the external yolk sac. The midgut region remains open to the yolk sac and will separate later. Foregut - Begins at the buccopharyngeal membrane, the foregut region in the head is now called the pharynx. At the lower end of the pharynx a ventral bud forms, that will later form the respiratory tract. Beneath this region the tube grows rapidly forming a dilation of the tube, that will later form the stomach. Beneath this region is the boundary of the foregut and ventrally lies the transverse septum. Midgut - Broadly open to the external yolk sac then with continued folding narrows to a ...
Studies on further attenuated live measles vaccine. I. Adaptation of measles virus to the chorioallantoic membrane of chick embryo and clinical tests on the str
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