1J9R: Alternate substrate binding modes to two mutant (D98N and H255N) forms of nitrite reductase from Alcaligenes faecalis S-6: structural model of a transient catalytic intermediate

Biomolecular Engineering, Georgia Institute of Technology,. Parker H. Petit Institute for Bioengineering and Bioscience,. Engineered Biosystems Building, 950 Atlantic Drive N.W., GA 30322, USA. #School of Chemistry ...

At present, Foreign Direct Investment (FDI) up to 49% is permitted in an Indian Joint Venture Company for setting up hardware, Up-linking HUB or Teleports and complying with guidelines of GOI and Ministry of Information and Broadcasting for Up-linking and broadcasting. India Government, Public Sector Mulla & Mulla & Craigie Blunt & Caroe 25 Jan 2006

Recently, the structure of a Cu-containing nitrite reductase (NiR) from Hyphomicrobium denitrificans (HdNiR) has been reported, establishing the existence of a new family of Cu-NiR where an additional

1l9s: Directing the mode of nitrite binding to a copper-containing nitrite reductase from Alcaligenes faecalis S-6: characterization of an active site isoleucine.

1ET7: Catalytic roles for two water bridged residues (Asp-98 and His-255) in the active site of copper-containing nitrite reductase.

The SCOP classification for the Quinohemoprotein amine dehydrogenase A chain, domain 3 superfamily including the families contained in it. Additional information provided includes InterPro annotation (if available), Functional annotation, and SUPERFAMILY links to genome assignments, alignments, domain combinations, taxonomic visualisation and hidden Markov model information.

The mechanism of the enzymatic degradation of poly([R]-3-hydroxybutyrate) (PHB) was investigated by using well-defined model substrates, including both linear and cyclic [R]-3-hydroxybutyrate (3HB) and [R]-3-hydroxyvalerate (3HV) oligomers, with two different PHB depolymerases. The linear and cyclic oligomers containing from 2 to 10 repeating units were hydrolyzed in solutions of the depolymerase isolated from Aspergillus fumigatus and Alcaligenes faecalis, and the rates of hydrolysis and types of products formed were characterized. Both of the depolymerases catalyzed the hydrolysis of the cyclic oligomers (macrolides) which contained more than three 3HB and 3HV repeating units. The degradation reactions of the linear and cyclic 3HB oligomers with the A. fumigatus depolymerase gave similar ratios of monomer-to-dimer products, but PHB itself formed mostly monomer on hydrolysis, indicating that the enzymatic hydrolysis reactions occurred by different mechanisms for these different types of ...

Phosphorus Compounds Inorganic Basic Chemicals: Producer of phosphate products in China. Products include labile calcium phosphate and food-grade tricalcium phosphate, as well as the salts of boron, magnesium, zinc, aluminum,

Reference: Raiskina M.E., Metabolism of macro-energy phosphorus compounds in the heart, Voprosy meditsinskoi khimii, 1959, vol: 5(2), 83-97 ...

Chemistry of Plant Phosphorus Compounds è un libro in lingua di Frank Arlen W. pubblicato da Elsevier Science Ltd nella collana Elsevier Science Ltd (Hardcover) - ISBN: 9780124071940

In a BIM for a mononuclear centre, the central atom (metal) is written first, followed by the endogenous ligands (amino acid residues), and then the exogenous ligands, e.g. water. If the ligating atom needs to be indicated to avoid ambiguity, the symbol for this is separated from the ligand symbol by a dot, e.g. NE.His stands for the Nε atom of a His residue. This is a simplistic description that does not take into account the stereochemistry at the metal atom. The polyhedral symbol is not mandatory for it may be unknown. It also does not make sense for polynuclear metal centres (see below).. Example 4.1 in Table 4 shows a mononuclear centre found in the blue copper protein azurin. In this centre, one copper atom is coordinated by the Nδ atoms of two His residues, one mainchain oxygen derived from Gly, one Sδ atom of a Met residue and one Sγ atom of a Cys residue. The coordination geometry is trigonal bipyramidal (TBPY-5; the polyhedral symbols used are as in Table S3 in Additional file ...

The bacterial redox protein azurin has been shown to be able to enter into cancer cells and induce apoptosis by stabilizing p53. Although the formation of a complex between the two proteins has been demonstrated, little is known about their binding ...

Fryzuk, M. D. ; Johnson, S. A. ; Patrick, B. O. ; Albinati, A. ; Mason, S. A. ; Koetzle, T. F. New Mode Of Coordination For The Dinitrogen Ligand: Formation, Bonding, And Reactivity Of A Tantalum Complex With A Bridging N-2 Unit That Is Both Side-On And End-On. Journal of the American Chemical Society 2001, 123, 3960-3973. ...

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Members of the Acidovorax genus are frequently encountered in association with PAH degradation, notably that of phenanthrene, but prior to this work little was known of the underlying genetic determinants behind the phenotype. The particular Acidovorax strain used in this study has genes very similar in sequence and arrangement to those in Alcaligenes faecalis AFK2, and the initial dioxygenase sequence is also highly similar to those in several Burkholderia strains, although not to that of Burkholderia sp. strain RP007. These observations suggest that this particular genotype may be widespread among some PAH-degrading members of the order Burkholderiales.. Unfortunately for comparative purposes, while the genes determined in this study bear significant resemblance to the Alcaligenes faecalis AFK2 sequences deposited in GenBank, there does not appear to be a publication associated with the entry (GenBank accession number AB024945). However, other previously published research on AFK2 revealed the ...

TWENTY FIVE Aspergillus isolates were screened from Giza Governorate and Saint Catherine Protectorate soils in Egypt. The antimicrobial activity of the crude extracts was tested against two Gram positive bacteria (Bacillus subtilis NRRL-B-4219, Staphylococcus aureus ATCC29213), four Gram negative bacteria (Alcaligenes faecalis B-170, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC10131, Pseudomonas aeruginosa ATCC27953), and one yeast (Candida albicans ATCC10231). The antioxidant activity using free radical scavenging model was assayed for the crude extracts. The antitumor activity for all of crude extracts was determined against HCT116 (Colon carcinoma cell line), HEPG2 (Liver carcinoma cell line), and MCF-7 (Breast carcinoma cell line). Aspergillus nomius was the most potent fungal species accordingly, it was chosen for bioactivity assay. Identification of this species was further confirmed at the molecular level based on nuclear ribosomal DNA 18s identities. An accession number, LC199488, was

The Tg2576 mouse, which carries the Swedish mutant form of human beta-amyloid precursor protein (hAPP(swe)), develops Alzheimers Disease (AD)-like phenotype (synaptic pathology, cognitive impairment and beta amyloid -A beta-plaques.) in the absence of significant neuronal loss. We have analyzed the hippocampal proteome of Tg2576, focusing on changes at 7 months of age, when A beta levels begin to increase but cognitive symptoms are still not evident, and at 16 months, when most AD-like features are manifested. Proteins differentially expressed with respect to wild-type animals were grouped according to their biological function and assessed in the context of AD. Metabolic enzymes, propionyl-CoA carboxylase, which has not been previously related to AD, and glutamine synthetase, which is a key enzyme for ammonium removal, were among deregulated proteins. Mitochondria of young animals have to cope with the metabolic stress and elevated ATP demand caused by overexpression of hAPP(swe). ...

Biochemists at the University of Georgia have discovered an unusual phosphorus-containing compound in an extreme heat-loving bacterium -- a discovery that will advance thinking about how life exists near the boiling point in underwater volcanic vents

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Role of arginine residues of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6.: To investigate the role of arginine in the folding of d-amin

A method for depositing copper on a titanium-containing surface of a substrate is provided. The method includes forming a patterned catalyst material on the substrate, such that the titanium-containing surface is exposed in selected regions. The catalyst material has an oxidation half-reaction potential having a magnitude that is greater than a magnitude of a reduction half-reaction potential of titanium dioxide. Copper is then deposited from an electroless solution onto the exposed regions of the titanium-containing surface.

We havent talked much yet about properly balancing half-reactions, but I know that there are a lot more steps involved, such as adding H2O to balance any oxygens and adding H+ to balance any hydrogens. The number of electrons you add should be to properly balance the charges on both sides, and yes the number of electrons for each half-reaction should equal each other (you can multiply one half-reaction by a certain factor to get them to equal each other) so that they cancel out. Prof Lavelle might discuss this further (or not!) so I wouldnt worry too much about it ...

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Special guest DJ Pioneer joins us for Part II of MC Kie Presents on GetDarkerTV, alongside live Sax from CJ Turner & Bongo Ben on the drums. […]. ...

TY - JOUR. T1 - A comparison of the mechanism for the reductive half-reaction between pea seedling and other copper amine oxidases (CAOs). AU - Prabhakar, Rajeev. AU - Siegbahn, Per E.M.. PY - 2003/7/30. Y1 - 2003/7/30. N2 - In a previous DFT study a mechanism for the reductive half-reaction of pea seedling amine oxidase (PSAO) was suggested. In many of the suggested steps a lysine at the active site plays an important role. However, this lysine is not found in other amine oxidases. The primary aim of the present DFT study is therefore to investigate alternative mechanisms for those amine oxidases (CAO) where the lysine residue is not present. One of the most important roles suggested for the lysine in PSAO was to protonate the O2-site of TPQ before the critical C-H bond cleavage of the substrate. In the absence of lysine the O2-site of TPQ is now suggested to be protonated by a water ligand on the copper metal complex, in line with experimental suggestions. In other steps the role of lysine is ...

Content is to be added here. Family GH128 contains b-1,3-glucanases that cleave b-1,3 linkages in various b-glucans such as lentinan from Lentinula edodes, laminarin from Laminaria digitata, pachyman from Poria cocos and curdlan from Alcaligenes faecalis. The first GH128 enzyme, GLU1, was cloned from L. edodes fruiting bodies (shiitake mushroom). GLU1 did not degrade b-1,3-linkages within b-1,3-1,4-glucans such as barley glucan, indicating the enzyme is categorized into EC 3.2.1.39 [1]. ...

TY - JOUR. T1 - Susceptibilities of enterococci to twelve antibiotics. AU - Tofte, R. W.. AU - Solliday, J. A.. AU - Crossley, K. B.. PY - 1984. Y1 - 1984. N2 - The susceptibilities of 347 urine isolates of enterococci (Streptococcus faecalis, 44%; S. faecalis subsp. zymogenes, 37%; S. faecalis subsp. liquefaciens, 19%) to ampicillin, azlocillin, mezlocillin, piperacillin, vancomycin, gentamicin, erythromycin, rosaramicin, rifampin, rifampin plus trimethoprim (1:4), trimethoprim-sulfamethoxazole (1:20), and chloramphenicol were determined by the agar dilution technique. There were no significant differences in susceptibility to individual agents among the subspecies of S. faecalis. Azlocillin and mezlocillin (MIC for 90% of isolates, 0.78 μg/ml) and piperacillin, ampicillin and vancomycin (MIC for 90% of isolates, 1.56 μg/ml) were the most active agents and were significantly more potent than the other reference antibiotics tested.. AB - The susceptibilities of 347 urine isolates of ...

It is generally assumed that tethering enhances rates of electron harvesting and delivery to active sites in multidomain enzymes by proximity and sampling mechanisms. Here, we explore this idea in a tethered 3-domain, trimeric copper-containing nitrite reductase. By reverse engineering, we find that …

An electroconductive polymer composition of the invention contains a conductive polymer and a trivalent phosphorus compound. The trivalent phosphorus compound is preferably a compound represented by

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Looking for online definition of Achromobacter xylosoxidans in the Medical Dictionary? Achromobacter xylosoxidans explanation free. What is Achromobacter xylosoxidans? Meaning of Achromobacter xylosoxidans medical term. What does Achromobacter xylosoxidans mean?

The oxidation and exchange reactions of simple phosphorus compounds and the behaviour of a range of low valent triphenylphosphine complexes of the transition metals in liquid hydrogen chloride at room temperature were studied by (^31)P nuclear magnetic resonance spectroscopy. When oxidation did occur, the phosphorus was oxidised from the +3 to the +5 oxidation state. In general, five co-ordinate fluorine containing phosphorus(V) species were produced from the oxidation of phosphorus(III) fluoride and four co-ordinate species of type PBr(_x)C1(_4-x)(^+) (O ≤ x ≤ 4) from the oxidation of phosphorus(ill) bromide or chloride. The (^31)P n.m.r. chemical shifts of the complete series PBr(_x)C1(_4-x)(^+) (O ≤ x ≤ 4) were recorded in solution for the first time, stabilised by tetrachloroborate anions in liquid hydrogen chloride. The protonation of a series of oxygen containing phosphorus (V) compounds in the solvent at room temperature was investigated using P n.m.r. spectroscopy. A measure of ...

penicillin G acylase, alpha-subunitpenicillin G acylase, beta-subunit4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACIDCALCIUM IONGLYCEROL

Pantoea agglomerans ATCC ® 33243D™ Designation: Genomic DNA from Pantoea agglomerans strain NCPPB 2971 TypeStrain=True Application:

Today industrial development trends are toward smaller, hotter, more compact and overloaded hydraulic systems with high power transfer requirements. This results in significantly extending the severity of operation of hydraulic equipment. Vanderbilt Chemicals, LLC offers a broad range of antioxidants, corrosion inhibitors, antiwear and extreme pressure additives to lubricate the critical hydraulic system components such as pump, control valves, motor / actuators. We also offer excellent friction reducers and antiwear to reduce friction and prevent wear of sliding vane, piston or gear pumps.

Alcaligenes Polysaccharides are manufactured by Hakuto under the tradename Alcasealan, and probably distributed in Europe by Nagase. It is described as a natural polysaccharides produced from fermentation. The polymer chain of Alcasealan forms a three-dimension network to hold free water as well as binding it. This 3D network has a unique characteristic which forms a moisturising film on the skin with unique lightweight texture. Alcasealan is widely used as emulsion and dispersion stabiliser; it also helps to suspense powders and glitters in cosmetics formulations. ...

Next-day shipping cDNA ORF clones derived from AT5G28350 Quinoprotein amine dehydrogenase, beta chain-like; RIC1-like guanyl-nucleotide exchange factor available at GenScript, starting from $99.00.

Sabale S. S.*, Koli A.D., Marathe R. J. and Phatake Y. B.. ABSTRACT. The secondary metabolites from microorganisms play a vital role in developing new chemotherapeutics. Microorganisms especially bacteria produce different secondary metabolites which had proved to be one of the most important source of lead compounds. In the present study potent azurin producing bacteria were isolated and screen from from three soil samples (Maharashtra, India) and identified by morphological, biochemical and molecular method (16s r DNA) as Pseudomonas aeruginosa which produce a biologically active protein azurin, that has array of bioactivities. The produced azurin was extracted and qualitatively characterized by using conventional and modern analytical methods. The spectroscopic study shows lambda max of produced protein as 225nm. Finally the production process of azurin was optimized by classical method. The selected strain of bacteria produces maximum azurin in selective media-I containing yeast extract and ...

Principal Investigator：HIRAMATSU Osamu, Project Period (FY)：1994 - 1995, Research Category：Grant-in-Aid for Developmental Scientific Research (B), Research Field：Biomedical engineering/Biological material science

PER-22 was intended to be a tool to help auditors in transfer pricing audits but has in fact led to an overcomplicated and burdensome process.

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Achromobacter xylosoxidans (formerly Alcaligenes xylosoxidans) is a Gram-negative, aerobic, oxidase and catalase-positive, motile bacterium with peritrichous flagella, from the genus Achromobacter. It is generally found in wet environments. Achromobacter xylosoxidans can cause infections such as bacteremia, especially in patients with cystic fibrosis. In 2013, the complete genome of an A. xylosoxidans strain from a patient with cystic fibrosis was sequenced. A. xylosoxidans is a Gram-negative rod that does not form spores. It is motile, with peritrichous flagella that distinguish it from Pseudomonas species, and is oxidase-positive, catalase-positive, and citrate-positive. It is urease and indole-negative. It produces acid oxidatively from xylose, but not from lactose, maltose, mannitol, or sucrose. It grows well on MacConkey agar and other inhibitory growth media such as deoxycholate, Salmonella-Shigella, and nalidixic acid-cetrimide agars. It is usually resistant to a variety of antibiotics ...

Shared Genotypes of Achromobacter xylosoxidans Strains Isolated from Patients at a Cystic Fibrosis Rehabilitation Center: During a study examining transmission

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Pantoea agglomerans HsvG protein: hsv - host-specific virulence factor; isolated from Erwinia herbicola; amino acid sequence in first source; GenBank U61275

InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

This is a water gelling agent made from a Gram-negative bacteria, similar to xanthan gum.. A 0.001% concentration can thicken up a product quite nicely, which is good because it costs more than $10 000 for a kilo.. Beyond thickening water, it also has moisturizing properties, similar to hyaluronic acid.. Is it better or worth the money? Im not sure - but if the popularity of this ingredient increases, the cost will go down. Much of the cost is the low yield from production. You can read more about how its produced here.. Japanese and Korean suppliers tend to have many unique and interesting raw materials, from my experience.. Its found in a few commercial products, which are very luxury and expensive (as is to be expected).. ...