TY - JOUR. T1 - Age-related susceptibility to apoptosis in human retinal pigment epithelial cells is triggered by disruption of p53-Mdm2 association.. AU - Bhattacharya, Sujoy. AU - Chaum, Edward. AU - Johnson, Dianna A.. AU - Johnson, Leonard R.. PY - 2012/1/1. Y1 - 2012/1/1. N2 - Relatively little is known about the contribution of p53/Mdm2 pathway in apoptosis of retinal pigment epithelial (RPE) cells or its possible link to dysfunction of aging RPE or to related blinding disorders such as age-related macular degeneration (AMD). Age-associated changes in p53 activation were evaluated in primary RPE cultures from human donor eyes of various ages. Apoptosis was evaluated by activation of caspases and DNA fragmentation. Gene-specific small interfering RNA was used to knock down expression of p53. We observed that the basal rate of p53-dependent apoptosis increased in an age-dependent manner in human RPE. The age-dependent increase in apoptosis was linked to alterations in several aspects of the ...
Age-related immune dysfunction presents serious health concerns for todays society, as the population of individuals over the age of 65 years old continues to expand. The consequences of immunosenescence are obvious, as aged individuals are less able to ward off bacterial, viral, and fungal infections, have higher incidences of cancer, and have overall decreased responses to protective vaccines compared with younger individuals (4, 14). Age-related changes in adaptive immunity are well documented, whereas less is known about the effects of age on the innate immune system, with particular regard to innate lymphocytes such as CD1d-restricted NKT cells. Here, we show that as age advances, the number of CD1d-restricted NKT cells increases and that these cells in the aged immune microenvironment actively suppress, rather than support efferent T cell immunity. Additionally, our findings support the concept that NKT cells may suppress efferent T cell immunity via mechanisms that involve excess ...
TY - JOUR. T1 - Evaluating convergence of within-person change and between-person age differences in age-heterogeneous longitudinal studies. AU - Sliwinski, Martin. AU - Hoffman, Lesa. AU - Hofer, Scott. PY - 2010/1. Y1 - 2010/1. N2 - The distinction of between-person age differences from within-person age changes is necessary for understanding aging-related change processes. Although longitudinal studies are required to address issues relating to within-person change, most studies begin using age-heterogeneous samples and conclude using survival-heterogeneous samples. Given the numerous potential confounds associated with age-heterogeneous samples, careful treatment of between-person age differences is essential to obtain the correct inferences regarding within-person age change. The authors demonstrate how failure to differentiate between-person age effects (and by extension, of survival age or other effects producing sample heterogeneity) will lead to uninterpretable inferences regarding ...
In order to clarify the mechanism through which extract of Ginkgo biloba leaves (EGb) improves cognitive function, we examined the effects of EGb on cerebral blood oxygenation in the prefrontal cortex (PFC) and on performance during a working memory task, using near-infrared spectrometry (NIRS).. First, we evaluated differences in behavioral performance of the Sternberg working memory test (ST) and in the activation pattern of the PFC during ST between 15 young and 19 middle-aged healthy women. Then, we examined the effect of EGb (120 mg/day for 6 weeks) on ST performance and PFC activation pattern in the middle-aged group.. The middle-aged group exhibited a longer reaction time (RT) in ST than the young group and showed a different PFC activation pattern during ST, i.e., the middle-aged group showed bilateral activation while the young group showed right-dominant activation. In the middle-aged group, administration of EGb for 6 weeks shortened the RT of ST and changed the PFC activation pattern ...
TY - JOUR. T1 - Volumetric correlates of spatiotemporal working and recognition memory impairment in aged rhesus monkeys. AU - Shamy, Jul Lea. AU - Habeck, Christian. AU - Hof, Patrick R.. AU - Amaral, David G. AU - Fong, Sania G.. AU - Buonocore, Michael H.. AU - Stern, Yaakov. AU - Barnes, Carol A.. AU - Rapp, Peter R.. PY - 2011/7. Y1 - 2011/7. N2 - Spatiotemporal and recognition memory are affected by aging in humans and macaque monkeys. To investigate whether these deficits are coupled with atrophy of memory-related brain regions, T1-weighted magnetic resonance images were acquired and volumes of the cerebrum, ventricles, prefrontal cortex (PFC), calcarine cortex, hippocampus, and striatum were quantified in young and aged rhesus monkeys. Subjects were tested on a spatiotemporal memory procedure (delayed response [DR]) that requires the integrity of the PFC and a medial temporal lobe-dependent recognition memory task (delayed nonmatching to sample [DNMS]). Region of interest analyses ...
Trehalose is a disaccharide of glucose found in diverse organisms and is suggested to act as a stress protectant against heat, cold, desiccation, anoxia, and oxidation. Here, we demonstrate that treatment of [nematode worms of the species] Caenorhabditis elegans with trehalose starting from the young-adult stage extended the mean life span by over 30% without any side effects. Surprisingly, trehalose treatment starting even from the old-adult stage shortly thereafter retarded the age-associated decline in survivorship and extended the remaining life span by 60%. Demographic analyses of age-specific mortality rates revealed that trehalose extended the life span by lowering age-independent vulnerability. Moreover, trehalose increased the reproductive span and retarded the age-associated decrease in pharyngeal-pumping rate and the accumulation of lipofuscin.. .... The life span-extending effect of trehalose was abolished in long-lived insulin/IGF-1-like receptor (daf-2) mutants. ... These findings ...
Purpose: The mammalian target of rapamycin (mTOR)-mediated signaling network is a central regulator of aging. Inhibiting mTOR is a promising strategy for treating various age-related diseases including macular degeneration (AMD). The goals of our current study are to investigate whether mTOR signaling has age- and/or pathology-associated changes in the retinal pigment epithelium (RPE) and to explore the functional roles of mTOR pathway in RPE aging and degeneration.. Methods: Post-mitotic aging of cultured human RPE cells was induced by altering the medium nutrient compositions. Basal and stimulated activities of mTOR were determined by measuring the phosphorylation status of downstream substrate proteins including S6K and S6. Subcellular localization of mTOR was evaluated using flat mounts of RPE/choroid tissue obtained from wild type and knockout mice deficient of nuclear factor erythroid 2-related factor 2 (Nrf2) at different ages. The in vivo activity of mTOR was further assessed by ...
Abstract: : Purpose: To examine age-dependent differences in activities of antioxidant enzymes (catalase, glutathione peroxidase, superoxide dismutase) in rabbit corneas. Methods: Activities of superoxide dismutase, glutathione peroxidase and catalase were studied in corneas of normal eyes (albino rabbits) of various age (1 month - young eyes, 4 - 6 months - young adult eyes, 2.0 - 2.5 years - middle aged eyes, 3.1 - 5.1 years - aged eyes). Activities of glutathione peroxidase and superoxide dismutase were investigated biochemically in the scraped corneal epithelium. Catalase activity was examined histochemically in the corneal epithelium and endothelium. Results: Activities of corneal antioxidant enzymes differed according to the age of animals. Young corneas revealed lower activities of all enzymes investigated than young adult corneas, where enzyme activities reached the maximum. In middle aged corneas glutathione peroxidase and catalase remained approximately at the same levels as compared ...
TY - JOUR. T1 - Age-dependent changes i n i ntervertebral di sc cell mi tochondri a and bi oenergeti cs. AU - Hartman, R.. AU - Patil, P.. AU - Tisherman, R.. AU - St. Croix, C.. AU - Niedernhofer, L. J.. AU - Robbins, P. D.. AU - Ambrosio, F.. AU - Van Houten, B.. AU - Sowa, G.. AU - Vo, Nam. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Robust cellular bioenergetics is vital in the energy-demanding process of maintaining matrix homeostasis in the intervertebral disc. Age-related decline in disc cellular bioenergetics is hypothesised to contribute to the matrix homeostatic perturbation observed in intervertebral disc degeneration. The present study aimed to measure how ageing impacted disc cell mitochondria and bioenergetics. Age-related changes measured included matrix content and cellularity in disc tissue, as well as matrix synthesis, cell proliferation and senescence markers in cell cultures derived from annulus fibrosus (AF) and nucleus pulposus (NP) isolated from the discs of young (6-9 months) and ...
Age-dependent differences in pseudorabies virus neuropathogenesis and associated cytokine expression: The severity of clinical symptoms induced by pseudorabies
a number of studies have examined age-related differences in thermoregulatory control during prolonged exercise (range 30-90 min) in the heat [range: 30-49°C/20-60% relative humidity (RH)] (4, 11-13, 20, 23, 26, 29, 33, 34). Some studies reported no differences in thermoregulatory function (4, 20, 23, 29, 33), whereas others found significant age-related impairments in heat loss capacity (e.g., reduced local sweating rate/onset/sensitivity and/or greater increments in core and skin temperatures) (11-13, 26, 34). It is possible that these discrepancies reflect that in some studies, older adults were able to achieve heat balance, while in other studies, heat load exceeded their physiological maximal sweating capacity; hence, differences in local sweat rate and/or core temperature were evident. What these studies did not examine, however, is whether age-related impairments in heat loss capacity occur during exercise of short duration (i.e., 15 min) when the rate of heat storage has been shown to ...
TY - JOUR. T1 - T1 values of phosphomonoester and phosphocreatine of brain show no significant change during development. AU - Hida, K.. AU - Kwee, Ingrid. AU - Nakada, T.. PY - 1992. Y1 - 1992. N2 - Changes in apparent 31P spin-lattice relaxation times (T1) associated with brain development were investigated in rat pups. While analysis of variance showed strong age dependency in [PME]/[ATP] and [PCr]/[ATP], the T1 values of PME, PCr, and ATP did not show any age dependency. The studies indicate that in contrast to the data which indicated aging-related changes in the T1 values of PME, the observed changes in PME during neonatal development are likely to be quantitative in nature.. AB - Changes in apparent 31P spin-lattice relaxation times (T1) associated with brain development were investigated in rat pups. While analysis of variance showed strong age dependency in [PME]/[ATP] and [PCr]/[ATP], the T1 values of PME, PCr, and ATP did not show any age dependency. The studies indicate that in ...
Increasing evidence shows an association between the use of vitamin D and improvement in age-related cognitive decline. In this study, we investigated the possible mechanisms involved in the neuroprotective effects of vitamin D on age-related brain changes and cognitive function. Male F344 rats aged 20 months (old) and 6 months (young) were used and randomly assigned to either vitamin D supplementation or no supplementation (control). A total of n = 39 rats were used in the study. Rats were individually housed and the supplementation group received a subcutaneous injection of vitamin D (1, α25-dihydroxyvitamin D3) 42 I.U./Kg for 21 days. Control animals received equal volume of normal saline. Behavioral testing in water maze and spontaneous object recognition tasks started on day 14. Levels of interleukin (IL)-1β and IL-10 were quantified to assess inflammatory state. Also, beta amyloid (Aβ) clearance and Aβ load were measured. Our results show that: (1) aged rats demonstrated significant learning
Kirsty McAleese, PhD, of Newcastle University, Newcastle, UK, discusses her research into fibrinogen leakage in the white matter of Alzheimers disease and normal aged brains, and the implications of the findings for fibrinogen as a biomarker. Dr McAleese highlights the notable results for extravascular fibrinogen and cerebral small vessel disease pathology. This interview took place at the Alzheimers Research UK (ARUK) Conference 2019, held in Harrogate, UK.
Chapter 2 appears to be the equivalent of the peer reviewed version of the following article: Lenehan, M. E., Summers, M. J., Saunders, N. L., Summers, J. J. and Vickers, J. C., 2015. Relationship between education and age-related cognitive decline: a review of recent research, Psychogeriatrics, 15(2), 154-162, which has been published in final form at http://dx.doi.org/10.1111/psyg.12083 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.. Chapter 2 appears to be the equivalent of a post-print version of an article published as: Lenehan, M. E., Summers, M. J., Saunders, N. L., Summers, J. J., Ward, D.D., Ritchie, K., Vickers, J. C., 2015. Sending your grandparents to university increases cognitive reserve: the Tasmanian Healthy Brain Project, Neuropsychology, 30(5), 525-531. This chapter may not exactly replicate the final version published in the APA journal. It is not the copy of record. ...
Epilepsy is widely prevalent in the elderly populations. Besides unpredictable seizure attacks, co-morbidities like cognitive impairment, anxiety, depression and disturbed social behavior are severe in elderly subjects inflicted with epilepsy. Though anti- epileptic drugs (AED) are the most common means of treating TLE, undesirable side effects associated with them limit their usage. Thus, there is a pressing need to develop alternative non-invasive therapeutic strategies to treat elderly individuals with TLE. Environmental enrichment (EE) has been shown to be beneficial in immature and young epileptic rats. However, knowledge regarding the effects of EE in aged epileptic subjects has not garnered much attention. As aging is one of the predominant risk factor involved in seizure development, a non-invasive therapeutic alternative like EE for treating elderly patients with TLE will be extremely beneficial. The proposed hypotheses suggest possible non-invasive therapeutic approaches to mitigate ...
Human brain derived neurotrophic element (BDNF) acting through the tropomyosin-related kinase receptor B (TrkB) enhances neuromuscular transmission in the diaphragm muscle mass of adult mice, reflecting presynaptic effects. vehicle treatment. In early old age (18 months), presynaptic terminal volume decreased compared to 6 month older diaphragm NMJs (~20 %). Inhibition of TrkB kinase activity significantly decreased the presynaptic terminal volume (~20 %) and engine end-plate 2D planar area (~10 %), self-employed of age group. Inhibition of TrkB kinase activity in early old age significantly reduced overlap of pre- and post-synaptic constructions and improved the proportion of denervated NMJs (to ~20 %). Collectively these results support a period of susceptibility in early old age when BDNF/TrkB signaling at diaphragm NMJs helps the maintenance of NMJs structure and muscle mass innervation. software of 1118460-77-7 IC50 BDNF enhanced neuromuscular transmission in 18 month older mice, similar to ...
TY - JOUR. T1 - Functional mapping of the promoter region of the GNB2L1 human gene coding for RACK1 scaffold protein. AU - Del Vecchio, Igor. AU - Zuccotti, Annalisa. AU - Pisano, Federica. AU - Canneva, Fabio. AU - Lenzken, Silvia C.. AU - Rousset, Francoise. AU - Corsini, Emanuela. AU - Govoni, Stefano. AU - Racchi, Marco. PY - 2009/2/1. Y1 - 2009/2/1. N2 - RACK1 (Receptor for Activated C Kinase 1) is a scaffold protein for different kinases and membrane receptors. Previously, we characterized an age-dependent decline of RACK1 protein expression which could be counteracted with DHEA (dehydroepiandrosterone) [Corsini, E., et al. 2002. In vivo dehydroepiandrosterone restores age-associated defects in the protein kinase C signal transduction pathway and related functional responses. J. Immunol. 168, 1753-1758. and Corsini, E., et al. 2005. Age-related decline in RACK-1 expression in human leukocytes is correlated to plasma levels of dehydroepiandrosterone. J. Leukoc. Biol. 77, 247-256.]. ...
Intrinsic neuronal excitability has been reported to change during normal aging. The bed nucleus of the stria terminalis (BNST), a limbic forebrain structure, is involved in fear, stress and anxiety; behavioral features that exhibit age-dependent properties. To examine the effect of aging on intrinsic neuronal properties in BNST we compared patch clamp recordings from cohorts of female mice at two ages, 3-4 months (Young) and 29-30 months (Aged) focusing on 2 types of BNST neurons. Aged Type I neurons exhibited a hyperpolarized resting membrane potential (RMP) of circa -80 mV compared to circa -70 mV in the Young. A key finding in this study is a hyper-excitability of Type II neurons with age reflected in an increase in firing frequency in response to depolarizing current injections; activation of Type II neurons is believed to dampen anxiety like responses. Such age-related changes in intrinsic neurophysiological function are likely to modulate how the limbic system, acting via BNST, shapes ...
Objective To explore the modulating function of isomalto oligosaccharide on intestinal mucosal immunity of aging model mice and its possible mechanisms.Method 48 Kunming mice were randomly divided into 4 group: young control group,aging model group,IMO group and IMOLCM group.After the aging models were made by injection of D-galactose,the IMO group was given IMO while IMOLCM group was given IMO and lincomycin.Each group were killed after 30 days.We detected the quantity of intestinal flora by the method of quantitative detection;the level of sIgA of intestinal mucous membrane by the method of radioimmunity;the expression of the IgA+ plasma cell in mice′s intestinal mucous membrane by the immunohistochemical method.Result(1) Dysbacteria could be observed in aging mice;The level of sIgA of intestinal mucous membrane,IgA+ plasma cell in aging model group were lower than the young control group′s;(2) Dysbacteria had improwed in IMO group;The level of sIgA of intestinal mucous membrane,the number of IgA+
Balance disorders are frequent with aging. They are particularly important because they decrease social autonomy of the aged subjects and they often provoke falls. The cause is always multifactorial. There is evidence that aging affects multiple sensory inputs, as well as the muscoloskeletal system and central nervous system ability to perform sensorimotor integration. For the evaluation of decreased balance skills in elderly, a specific questionnaire has been prepared, in order to identify high risk of falling called falling risk inventory (FRI) questionnaire, and a complex psycho-sensory-motor test has been studied by means of posturography, in order to detect specific vestibular impairment. Regarding ethiopathogenesis of balance disorders in aged subjects, because the decline of behavioral and cognitive performances are due also to decline of biological rhythm control, the role of melatonin (the hormone regulating circadian rhythms, being strictly connected with cerebellar function, and it is ...
Can regular aerobic exercise diminish the damaging effects of aging on the vascular system by improving the health of vascular endothelial cells? In this insightful podcast, Associate Editor Nancy Kanagy interviews lead author Matthew Rossman (University of Colorado Boulder) and content expert Raymond Migrino (Phoenix VA Health Care System) about the work by Rossman et al focusing on age-related changes in endothelial cell senescence and associated changes in endothelial cell function that occurs with normal, healthy aging. Habitual exercise has been shown to reduce age-related phenotypic changes such as increased arterial stiffness and reduced endothelial cell function. Did Rossman and colleagues find that regular aerobic exercise in older adults ameliorated increases in endothelial cell senescence? Listen and learn more.. Matthew J. Rossman, Rachelle E. Kaplon, Sierra D. Hill, Molly N. McNamara, Jessica R. Santos-Parker, Gary L. Pierce, Douglas R. Seals, Anthony J. Donato Endothelial cell ...
Skeletal muscle exhibits remarkable plasticity in its ability to modulate its mass in response to the physiologic changes associated with functional use, systemic disease, and aging. Although a gradual loss of muscle mass normally occurs with advancing age, its increasingly rapid progression results in sarcopenia in a subset of individuals. The identities of muscle-enriched, long noncoding RNAs that regulate this process are unknown. Here, we identify a long noncoding RNA, named Chronos, whose expression in muscle is positively regulated with advancing age and negatively regulated during Akt1-mediated growth. Inhibition of Chronos induces myofiber hypertrophy both in vitro and in vivo, in part, through the epigenetic modulation of Bmp7 signaling. ...
Age is an important prognostic factor in both primary and secondary prevention of coronary heart disease. The progress in molecular biology resulted in the introduction of the notion of biological age which facilitates the objective estimation of the organisms regenerative potential. The purpose of this paper is to review the effects of several aspects of biological age on the development and progression of coronary heart disease. The term biological age is defined and its markers are characterised. The influence of risk factors for coronary heart disease and heart failure on biological age is also discussed. Special emphasis is placed on the clinical aspects of cellular senescence in car- diovascular system.
Scientists have found that the compound resveratrol slows age-related deterioration and functional decline of mice on a standard diet, but does not increase longevity when started at middle age. This study, conducted and supported in part by the National Institute on Aging, part of the National Institutes of Health, is a follow-up to 2006 findings that resveratrol improves health and longevity of overweight, aged mice.
A study exploring the impact of a healthy lifestyle on age-related macular degeneration (AMD) found that women whose diets were the healthiest (scored on the 2005 Healthy Eating Index) had…
TY - JOUR. T1 - Aging-related increase to inducible atrial fibrillation in the rat model. AU - Hayashi, Hideki. AU - Wang, Charles. AU - Miyauchi, Yasushi. AU - Omichi, Chikaya. AU - Pak, Hui Nam. AU - Zhou, Shengmei. AU - Ohara, Toshihiko. AU - Mandel, William J.. AU - Lin, Shien-Fong. AU - Fishbein, Michael C.. AU - Chen, Peng Sheng. AU - Karagueuzian, Hrayr S.. PY - 2002/1/1. Y1 - 2002/1/1. N2 - Introduction: Aging is associated with atrial interstitial fibrosis and increased incidence of atrial fibrillation (AF). We hypothesized that aged rats are suitable for study of aging-related AF and that partial atrial cellular uncoupling induced with heptanol in young rats mimics aging-related AF. Methods and Results: Interatrial conduction time and atrial response to burst atrial pacing were evaluated in 11 young (2-3 months) and 12 old (22-24 months) male rats (Fisher 344) in the Langendorff-perfused setting. At baseline, sustained (, 30 sec) atrial tachycardia (AT) and AF were induced in 10 of 12 ...
locus, play critical roles in inducing and maintaining permanent cell cycle arrest during cellular senescence. Although the elimination of p16INK4a-expressing cells extends the life span of the mouse, it is unclear whether tissue function is restored by the elimination of senescent cells in aged animals and whether and how p19ARF contributes to tissue aging. The aging-associated decline in lung function is characterized by an increase in compliance as well as pathogenic susceptibility to pulmonary diseases. We herein demonstrated that pulmonary function in 12-month-old mice was reversibly restored by the elimination of p19ARF-expressing cells. The ablation of p19ARF-expressing cells using a toxin receptor-mediated cell knockout system ameliorated aging-associated lung hypofunction. Furthermore, the aging-associated gene expression profile was reversed after the elimination of p19ARF. Our results indicate that the aging-associated decline in lung function was, at least partly, attributed to ...
Full Text - The neuropathogenesis of postoperative delirium remains mostly unknown. The gut microbiota is implicated in the pathogenesis of neurological disorders. We, therefore, set out to determine whether anesthesia/surgery causes age-dependent gut microbiota dysbiosis, changes in brain IL-6 level and mitochondrial function, leading to postoperative delirium-like behavior in mice. Female 9 or 18 months old mice received abdominal surgery under 1.4% isoflurane for two hours. The postoperative delirium-like behavior, gut microbiota, levels of brain IL-6, PSD-95 and synaptophysin, and mitochondrial function were determined by a battery of behavioral tests, 16s rRNA sequencing, ELISA, Western blot and Seahorse XFp Extracellular Flux Analyzer. Intragastric administration of Lactobacillus (10 days) and probiotic (20 days) were used to mitigate the anesthesia/surgery-induced changes. Anesthesia/surgery caused different alterations in gut microbiota, including change rate of reduction in the levels of gut
TY - JOUR. T1 - Age-related variations in flavonoid intake and sources in the Australian population. AU - Johannot, Lidwine. AU - Somerset, Shawn M.. PY - 2006/12/1. Y1 - 2006/12/1. N2 - Objective: To estimate flavonoid intake in the Australian population. Design: Flavonoid consumption was estimated from 24-hour recall data and apparent consumption data using US Department of Agriculture flavonoid composition data. Subjects: The National Nutrition Survey 1995 assessed dietary intake (24-hour recall) in a representative sample (n = 13858) of the Australian population aged 2 years and over. Results: Analysis of the 24-hour recall data indicated an average adult intake (, 18 years) of 454 mg day-1 (92% being flavan-3-ols). Apple was the highest quercetin source until age 16-18 years, after which onion became an increasingly important prominent source. Variations in hesperetin consumption reflected orange intake. Apple, apricot and grapes were the major sources of epicatechin and catechin for ...
The increasing number of the elderly in Indonesia as a result of the success of development has led to increasing problems in the elderly group whose natural life journey will experience an old age with all its limitations, especially in the health problems of the elderly who suffer from disabilities compared to younger people. The situation is also added that the elderly usually suffer from a variety of physiological disorders that are chronic, and biological aspect, psychological aspect, socio economic aspect will experience setback. The empowerment of elderly group is an effort to give power or strength so that elderly have independence, especially in aspects of intellectual ability, and aspects Physical, the empowerment of the elderly is done by improving ability through education. This study aims to analyze the influence of empowerment of the elderly group through health education on the independence of Activitiey Daily Living (ADL). The research design used quasi-experiment with the approach of
1. Fights the symptoms of aging naturally:. Aging can be the worst enemy for all those beauty conscious kings and queens out there, especially in the late 30s when everyones dreams are on the route of reaching their destination. When thousands of chemical-based products flood the market with the claim of guaranteed younger look, how will you feel if I say that the simple, natural and glorious fruit like Grapefruit can fight against all the symptoms of aging, just like that? Feeling awesome right!. FRTA or the Free radical theory of aging can assist you in understanding the real causes of aging. This theory states that organisms age because cells accumulate free radical damage over time. These free radicals cause oxidative damage in the cells, which pave way for aging and other age-related disorders like arthritis, diabetes, cancer, atherosclerosis and Alzheimers disease.. Numerous studies have proven that antioxidants are the best remedy for arresting the oxidative damage of cells by ...
Principal Investigator:MATSUNAGA Shunji, Project Period (FY):1998 - 1999, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Orthopaedic surgery
The neuropathological and immunohistochemical analysis found that the extent and distribution of Aβ deposits and NFTs/hyper-phosphorylated tau in the hippocampus is comparable between AD and NDAN cases (Table 1 and Figure 1). Additionally, Aβ1-42 levels are increased in NDAN similar to levels in AD and Aβ species can be detected by Western blot analysis in total protein extracts from both AD and NDAN hippocampi (Figure 1D & E). The presence of LMW Aβ species demonstrates that intact cognition could not be simply ascribed to efficient, or off-pathway aggregation of Aβ resulting in low levels or absence of Aβ oligomers.. When Aβ was assayed by Western blot in purified PSD fractions the results showed a robust presence of Aβ species in the AD cases, consistent with previous studies [26, 51]. However, there was a complete absence of LMW Aβ species at the PSD isolated from NDAN hippocampi (Figure 2C). These species were confirmed to be Aβ oligomers using a LMW Aβ oligomer specific ...
Q&A: Mid Back Pain in Young Individual Aged 45 Years, Know the Causes, Symptoms, Treatment options to get relief from Mid Back Pain.
Alzheimers disease (AD) is the most common form of dementia in elderly and is characterized by beta-amyloid (Abeta) aggregates forming plaques in the brain and neurofibrillary tangles. Abeta-oligomers in particular, cause neurotoxicity and cell death in AD brains. Abeta plaques in the AD brain are surrounded by activated microglia which implies an important role for these cells in the progression of AD. Therefore, microglia can be an interesting therapeutic target for AD but so far, no promising microglial therapies have been developed yet. It has been suggested that defects in synapses have a central role in age-associated changes in the central nervous system. Synapses are especially vulnerable to damage and age-related synaptic dysfunction is thought to precipitate neurological degeneration in neurodegenerative diseases such as AD.. Early in postnatal development neurons generate more synaptic connections than those that are retained in the adult brain tissue. Recently, it has been shown ...
So weve all seen it… the elderly male who presents with such an exaggerated forward-flexed posture it looks as though he will fall over if not for the cane propping up his torso with every step, or the middle aged woman whos hunch or kyphosis is accentuated further by her significant loss of lordosis, stiff pelvis, and short hamstrings. Certainly age related changes and conditions occur, we become stiff, our bones become brittle and our discs become more rigid and narrow. Aside from this, far too many cases of preventable postural deformities occur and unfortunately, once a person is in the later stages of life they are almost impossible to correct.. Of course posture related deformities are no longer exclusive to the older population, it is now more prevalent than ever in our youth. Children and teenagers are receiving increased medical care for posture related conditions; chronic back pain is at the top of the list. I see far too many young men and women with significant slouched ...
Decreased flexibility is involved in the aetiology of a range of physical impairments. Loss of flexibility occurs as we age, although to date no causal relationships have been determined. Degenerative changes are exacerbated by disuse and the development of osteoarthritis, osteoporosis and atherosclerosis. Age related changes in musculoskeletal tissue and increased incidence of joint pathology can negatively affect joint extensibility and stiffness. Flexibility exercises have been recommended to be performed at least twice a week, as unique exercises or as part of a physical activity program (Cress, Buchner et al. 2005). Overall, physical activity interventions emphasising flexibility have demonstrated positive effects on older peoples range of movement (ROM) and physical function, even in frail older people (McMurdo and Rennie 1993). A review of flexibility and physical function in older people noted the paucity of evidence about specific or general physical activity programs to improve joint ...
TY - JOUR. T1 - The aging of hematopoietic stem cells. AU - Morrison, Sean J.. AU - Wandycz, Antoni M.. AU - Akashi, Koichi. AU - Globerson, Amiela. AU - Weissman, Irving L.. PY - 1996/9/1. Y1 - 1996/9/1. N2 - We have purified hematopoietic stem cells (HSCs) from the bone marrow of old mice and compared their properties to HSCs in young and middle-aged mice. Single, reconstituting HSCs (by limit dilution) from old and young mice exhibited indistinguishable progenitor activities in vivo. HSCs were five times as frequent in the bone marrow of old mice; however, HSCs from old mice were only one-quarter as efficient at homing to and engrafting the bone marrow of irradiated recipients. HSCs in young and middle-aged mice rarely were in the S/G2/M phases of the cell cycle, but HSCs in old mice were frequently in cycle. We speculate that the unexpected proliferation of HSCs in old mice might be related to the increased incidence of leukemia in old mice. HSCs change with age, but it is unknown whether ...
Lifelong regular physical activity is associated with reduced risk of type 2 diabetes (T2D), maintenance of muscle mass and increased metabolic capacity. However, little is known about epigenetic mechanisms that might contribute to these beneficial effects in aged individuals. We investigated the effect of lifelong physical activity on global DNA methylation patterns in skeletal muscle of healthy aged men, who had either performed regular exercise or remained sedentary their entire lives (average age 62 years). DNA methylation was significantly lower in 714 promoters of the physically active than inactive men while methylation of introns, exons and CpG islands was similar in the two groups. Promoters for genes encoding critical insulin-responsive enzymes in glycogen metabolism, glycolysis and TCA cycle were hypomethylated in active relative to inactive men. Hypomethylation was also found in promoters of myosin light chain, dystrophin, actin polymerization, PAK regulatory genes and oxidative ...
Loss of immune function and an increased incidence of myeloid leukemia are two of the most clinically significant consequences of aging of the hematopoietic system. To better understand the mechanisms underlying hematopoietic aging, we evaluated the cell intrinsic functional and molecular properties of highly purified long-term hematopoietic stem cells (LT-HSCs) from young and old mice. We found that LT-HSC aging was accompanied by cell autonomous changes, including increased stem cell self-renewal, differential capacity to generate committed myeloid and lymphoid progenitors, and diminished lymphoid potential. Expression profiling revealed that LT-HSC aging was accompanied by the systemic down-regulation of genes mediating lymphoid specification and function and up-regulation of genes involved in specifying myeloid fate and function. Moreover, LT-HSCs from old mice expressed elevated levels of many genes involved in leukemic transformation. These data support a model in which age-dependent alterations
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This section examines evidence for neurovascular changes during normal ageing and for neurovascular and/or BBB dysfunction in various neurodegenerative diseases, as well as the possibility that vascular defects can precede neuronal changes.. Age-associated neurovascular changes. Normal ageing diminishes brain circulatory functions, including a detectable decay of CBF in the limbic and association cortices that has been suggested to underlie age-related cognitive changes77. Alterations in the cerebral microvasculature, but not changes in neural activity, have been shown to lead to age-dependent reductions in functional hyperaemia in the visual system in cats78 and in the sensorimotor cortex in pericyte-deficient mice33. Importantly, a recent longitudinal CBF study in neurologically normal individuals revealed that people bearing the apolipoprotein E (APOE) ɛ4allele - the major genetic risk factor for late-onset Alzheimers disease79, 80, 81 - showed greater regional CBF decline in brain regions ...
Zika virus (ZIKV) is primarily transmitted to humans by Aedes aegypti and Ae. albopictus. Vector-virus interactions influencing vector competence vary and depend on biological and environmental factors. A mosquitos chronological age may impact its immune response against virus infection. Insecticides, source reduction, and/or public education are currently the best defense against mosquitoes that transmit ZIKV. This study assessed the effects of a mosquitos chronological age at time of infection on its response to ZIKV infection. We exposed young (6-7 d post-emergence) and old (11-12 d post-emergence) Ae. albopictus to a sublethal dose of bifenthrin prior to oral exposure to blood meals containing ZIKV (7-day incubation period). Old mosquitoes experienced a significantly (p < 0.01) higher rate of mortality than young mosquitoes. Significantly higher ZIKV body titers (p < 0.01) were observed in the old control group compared to the young control group. Significantly higher (p < 0.01) ZIKV
From Cell Stem CellBy Stuart P. Atkinson Oligodendrocytes precursor cells (OPCs) differentiate into oligodendrocytes with remyelination capabilities which, in the adult central nervous system (CNS), restores conduction, prevents axonal degradation and promotes functional recovery. Reduction in this capacity in aging (Sim et al) leads to demyelinated neurons and axonal degeneration, which is understood to be mediated in part by environmental signals (Hinks and Franklin). This suggests that exogenous factors may be able to reverse this age-associated decline in function, which has now been addressed in an article (Ruckh and Zhao et al) in Cell Stem Cell by researchers in the laboratory of Amy J. Wagers (Howard Hughes Medical Institute) and Robin J.M. Franklin (MRC Centre for Stem Cell Biology and Regenerative Medicine).
The overall goals of the Laboratory of Cardiovascular Science are (1) to identify age-associated changes that occur within the cardiovascular system and to determine the mechanisms for these changes; (2) to study myocardial structure and function and to determine how age interacts with chronic disease states to alter function; (3) to study basic mechanisms in excitation-contraction coupling and how these are modulated by surface receptor signaling pathways in cardiac muscle; (4) to determine the chemical nature and sequence of intermediate reactions controlling the movement of ions through ionic channels and pumps present in myocardium, and how these are affected by aging and disease; (5) to determine mechanisms that govern neuro-hormonal behavioral aspects of hypertension; (6) to determine mechanisms of normal and abnormal function of vascular smooth muscle and endothelial cells; and (7) to establish the potentials and limitations of new therapeutic approaches such as gene transfer techniques. ...
TY - JOUR. T1 - Total free amino acid levels in adult and senescent rat liver. AU - Eichholz, R. L.. AU - Buetow, D. E.. PY - 1978. Y1 - 1978. N2 - The total free amino acid content per gram of liver wet weight does not differ significantly between adult and senescent female rats, being 28.8 ± 3.3 μmol in adult (13-17 months) and 26.2 ± 2.2 μmol in senescent (23-29 months) animals. This is the first study in which these two age-groups of rats have been so compared. These results combined with a survey of the literature show that amino acid levels in rat liver do not change much, if at all, throughout the lifespan of the animal.. AB - The total free amino acid content per gram of liver wet weight does not differ significantly between adult and senescent female rats, being 28.8 ± 3.3 μmol in adult (13-17 months) and 26.2 ± 2.2 μmol in senescent (23-29 months) animals. This is the first study in which these two age-groups of rats have been so compared. These results combined with a survey ...
Brain aging is a natural process, but for many, the aging brain causing levels of mental dysfunction that often reduce the quality and length of life. Loss of short and long-term memory, increased inability to find the right words or connect something you are looking at and recalling its name, mental fogginess, outright dementia and Alzheimers disease and an overall sense of fatigue are just some of the ways in which an aging brain could affect you.. Below are some of my thoughts regarding nutritional and natural ways to offset the degenerative effects of aging upon the human brain that Integrated Medicine of Mount Kisco have researched and perfected for use with our large patient population. These are general considerations to help prevent certain age related changes and to offset those that may be inevitable. It is essential to keep in mind that careful medical and nutritional history, dietary review, personalized laboratory (i.e., blood analysis and other testing), brain imaging (i.e., ...
Life startles at conception. Immediately fertilization takes place changes and fonts occur that go out hold the physical body of person to be born. This enquiry tries to find out the force play of aboriginal life on the later life of an individual. And if early life actuates the later life of the individual, then do pincerren who grow up in crimson communities capture a tendency to exhibit violent looks as adults? The research will apply the cross-sectional design. People who differ in mature argon studied at the very(prenominal) magazine. A comparison of age related changes and the social behavior is make.A hypothesis is put out front and a cross-sectional experiment is done to test it. One advantage of this design is that one burn down collect data from different ages of squirtren over a short period of time. Stages of human development subsequently fertilization takes place, the zygote is formed. The zygote undergoes cell division some hours after it is formed. It takes one week to telescope ...
Published on: October 27, 2014. by Medical XPress:. Dietary cocoa flavanols-naturally occurring bioactives found in cocoa-reversed age-related memory decline in healthy older adults, according to a study led by Columbia University Medical Center (CUMC) scientists. The study, published today in the advance online issue of Nature Neuroscience, provides the first direct evidence that one component of age-related memory decline in humans is caused by changes in a specific region of the brain and that this form of memory decline can be improved by a dietary intervention.. As people age, they typically show some decline in cognitive abilities, including learning and remembering such things as the names of new acquaintances or where one parked the car or placed ones keys. This normal age-related memory decline starts in early adulthood but usually does not have any noticeable impact on quality of life until people reach their fifties or sixties. Age-related memory decline is different from the ...
TY - JOUR. T1 - Chronic melatonin treatment prevents age-dependent cardiac mitochondrial dysfunction in senescence-accelerated mice. AU - Rodríguez, María I.. AU - Carretero, Miguel. AU - Escames, Germaine. AU - López, Luis C.. AU - Maldonado, María D.. AU - Tan, Dun Xian. AU - Reiter, Russel J.. AU - Acuña-Castroviejo, Darío. N1 - Funding Information: This work was supported in part by the Instituto de Salud Carlos III (ISCIII, Spain) through grants G03/137, PI02-1447 and PI03-0817. MIR and MC are predoctoral fellows from the ISCIII, and LCL is a postdoctoral fellow from the Ministerio de Educación (Spain). Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2007/1. Y1 - 2007/1. N2 - Heart mitochondria from female senescence-accelerated (SAMP8) and senescence-resistant (SAMR1) mice of 5 or 10 months of age, were studied. Mitochondrial oxidative stress was determined by measuring the levels of lipid peroxidation, glutathione and glutathione disulfide and glutathione ...
Exercise training and beta-blocker treatment ameliorate age-dependent impairment of beta-adrenergic receptor signaling and enhance cardiac responsiveness to adrenergic stimulation. Am J Physiol Heart Circ Physiol 293: H1596-H1603, 2007. First published June 8, 2007; doi:10.1152/ajpheart.00308.2007.- Cardiac beta-adrenergic receptor (*-AR) signaling and left ventricular (LV) responses to beta-AR stimulation are impaired with aging. It is shown that exercise and beta-AR blockade have a favorable effect on cardiac and vascular *-AR signaling in several cardiovascular diseases. In the present study, we examined the effects of these two different strategies on *-AR dysregulation and LV inotropic reserve in the aging heart. Forty male Wistar-Kyoto aged rats were randomized to sedentary, exercise (12 wk treadmill training), metoprolol (250 mg kg-1 day-1 for 4 wk), and exercise plus metoprolol treatment protocols. Ten male Wistar- Kyoto sedentary young rats were also used as a control group. Old ...
Previously, the word healthspan has been used in many scientific contexts, but the definition has been ambiguous at best. Therefore, in this study, we focused on providing a conceptual framework of healthspan that can be easily verified in a laboratory setting using C. elegans. The current study makes no claim that the healthspan parameters tested cover all aspects of healthspan, nor does it make any assumptions that antiaging therapies that extend lifespan cannot coincide with an increase in healthspan. Rather, we highlight limitations of aging studies focused solely on lifespan extension.. Here we defined healthspan broadly by testing multiple assays as the animals aged. We tested wild type as our benchmark and then compared data from four long-lived mutants with wild-type health. From these data, we calculated and compared both the chronological number of days an animal is healthy vs. frail and the percent of the physiological lifespan the animal spends in each state (healthspan:gerospan ...
TY - JOUR. T1 - Ablation of Neurogenesis Attenuates Recovery of Motor Function after Focal Cerebral Ischemia in Middle-Aged Mice. AU - Sun, Fen. AU - Wang, Xiaomei. AU - Mao, Xiao Ou. AU - Xie, Lin. AU - Jin, Kunlin. PY - 2012/10/26. Y1 - 2012/10/26. N2 - Depletion of neurogenesis worsens functional outcome in young-adult mice after focal cerebral ischemia, but whether a similar effect occurs in older mice is unknown. Using middle-aged (12-month-old) transgenic (DCX-TK(+)) mice that express herpes simplex virus thymidine kinase (HSV-TK) under control of the doublecortin (DCX) promoter, we conditionally depleted DCX-positive cells in the subventricular zone (SVZ) and hippocampus by treatment with ganciclovir (GCV) for 14 days. Focal cerebral ischemia was induced by permanent occlusion of the middle cerebral artery (MCAO) or occlusion of the distal segment of middle cerebral artery (dMCAO) on day 14 of vehicle or GCV treatment and mice were killed 24 hr or 12 weeks later. Increased infarct volume ...
Life Extension Immune Senescene Protection Formula contains Reishi Mushroom Extract & Cistanche Tubulosa Extract. These two Mushrooms are known for their inherent ability to aid in a healthy Immune System. Introducing Life Extension Immune Senescence Protection Formula!!
AGEs have been shown to accumulate in diabetic and ageing organs, including ocular tissues (cornea, lens,vitreous, and retina). Accumulation of AGEs in ECM was shown to elicit several changes of ECM including decreased solubility, decreased susceptibility to enzymes, and changes in such properties as thermal stability, mechanical strength, and stiffness. These changes in the physicochemical properties of ECM are believed to contribute, in part, to the development of age related changes and diabetic complications, including cardiovascular disease, retinopathy, nephropathy, indurated skin and joint stiffness. Albonet al reported a linear age related increase of pentocidine, an advanced glycation end product, in lamina cribrosa. Accumulation of AGEs in ECM of optic nerve heads in the elderly may decrease elasticity of lamina cribrosa and compromise the ability of cribriform plates to bear the strain caused by elevated intraocular pressure. The results of the current study showed that accumulation ...
Recently, research has focused intensely on age-related tissue changes, not only in the field of aesthetic dermatology but also in the dental field. Judging from clinical experience, relevant changes were suspected in the periodontal tissue thus influencing orthodontic tooth movement and the progression of periodontal disease.. Evidence was found that orthodontic tooth movement seems to be impaired in adult patients, thus requiring different treatment protocols than in adolescent patients. These findings will be discussed more thoroughly later in this article.. Periodontal disease however seems to be less dependent on age [1]. A study comparing experimental gingivitis in young vs. old individuals by Fransson et al. [2, 3] did confirm that older individuals experience more severe inflammation in response to experimental gingivitis. Nevertheless, several studies evaluating whether age is a risk factor for increased loss of periodontal support, have shown that aging either has no effect at all or, ...
TY - JOUR. T1 - Energy Metabolism and the Burden of Multimorbidity in Older Adults. T2 - Results From the Baltimore Longitudinal Study of Aging. AU - Fabbri, Elisa. AU - An, Yang. AU - Schrack, Jennifer A.. AU - Gonzalez-Freire, Marta. AU - Zoli, Marco. AU - Simonsick, Eleanor M.. AU - Guralnik, Jack M.. AU - Boyd, Cynthia M.. AU - Studenski, Stephanie A.. AU - Ferrucci, Luigi. N1 - Publisher Copyright: © 2014 The Author.. PY - 2015/11. Y1 - 2015/11. N2 - Excessively elevated resting metabolic rate (RMR) for persons of a certain age, sex, and body composition is a mortality risk factor. Whether elevated RMR constitutes an early marker of health deterioration in older adult has not been fully investigated. Using data from the Baltimore Longitudinal Study of Aging, we hypothesized that higher RMR (i) was cross-sectionally associated with higher multimorbidity and (ii) predicted higher multimorbidity in subsequent follow-ups. The analysis included 695 Baltimore Longitudinal Study of Aging ...
A cross-sectional and a longitudinal design were employed to address whether PDAPP mice exhibit any plaque-related learning deficit. Firstly, the cross-sectional study indicated that PDAPP mice simultaneously displayed an early (plaque-independent) and age-related learning impairment. Further analysis showed that the age-related learning deficit was highly correlated with plaque burden in the hippocampus of aged PDAPP mice, suggesting that amyloid plaques play a very important role in memory loss of AD. Second, the longitudinal study showed that the same PDAPP mice exhibited significant age-related learning deficits in trials to criterion and learning capacity tasks when they aged. Interestingly, cued navigation and object recognition in both cross-sectional and longitudinal studies were unaffected, indicating normal sensorimotor function and recognition memory of PDAPP mice. The longitudinal study further showed that the age-related learning impairment was significantly correlated with ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
TY - JOUR. T1 - Age-related changes in the numbers of mammotrophs, somatotrophs and mammosomatotrophs in the anterior pituitary gland of female rats. T2 - A flow cytometric study. AU - Shinkai, Tadashi. AU - Sakurai, Yoko. AU - Ooka, Hiroshi. PY - 1995/9/15. Y1 - 1995/9/15. N2 - Age-related changes in the numbers of mammotrophs, somatotrophs, mammosomatotrophs, and the cells of other types in the anterior pituitary of female Wistar rat were measured by flow cytometry. The mammotrophs increased with age, and the somatotrophs decreased with senescence. The mammosomatotrophs increased remarkably in senescent rats, and these cells of the rats older than 21 months were about 10 times more than those of 3, and 12-13 months old rats. This result indicates that the stability of gene expression in cell differentiation is reduced in the aging process of the anterior pituitary.. AB - Age-related changes in the numbers of mammotrophs, somatotrophs, mammosomatotrophs, and the cells of other types in the ...
Aging is the greatest risk factor for developing neurodegenerative diseases, which are associated with diminished neurotransmission as well as neuronal structure and function. However, several traits seemingly evolved to avert or delay age-related deterioration in the brain of the longest-lived rodent, the naked mole-rat (NMR). The NMR remarkably also exhibits negligible senescence, maintaining an extended healthspan for ~75 % of its life span. Using a proteomic approach, statistically significant changes with age in expression and/or phosphorylation levels of proteins associated with neurite outgrowth and neurotransmission were identified in the brain of the NMR and include: cofilin-1; collapsin response mediator protein 2; actin depolymerizing factor; spectrin alpha chain; septin-7; syntaxin-binding protein 1; synapsin-2 isoform IIB; and dynamin 1 ...
Although isolated mitochondria experiments showed that aging-related dysfunction of mitochondrial respiration may prevent APC, isoflurane could still protect isolated intact aged human cardiomyocytes from stress-induced cell death. Indeed, pretreatment of MA myocytes with isoflurane improved cell survival during acute oxidative stress, whereas this effect was much less pronounced in OA myocytes, suggesting that aging-related changes in mitochondrial function could affect viability of MA and OA myocytes. The involvement of another potential effector of APC, the sarcKATPchannel,14 in human cardiomyocyte protection was tested using HMR-1098, the sarcKATPchannel blocker. HMR-1098 attenuated the protection by isoflurane, suggesting that in adult human atrial myocytes, protection from stress is mediated partly via sarcKATPchannel. The properties of single sarcKATPchannels from adult human right atrial myocytes, amplitude of unitary KATPcurrent, slope conductance at negative membrane potentials, and ...
The CSF represents the reciprocal of the minimum contrast required to detect sinusoidal gratings that vary in spatial frequency (the number of cycles per degree of visual angle, cpd). As shown in the top panel of figure 3, scotopic CSFs were found to be low pass for all age groups. Statistically significant age related declines in contrast sensitivities were found for spatial frequencies at or below 1.2 cpd. This greater loss in contrast sensitivity at low spatial frequencies is quite different from photopic contrast sensitivity changes with age in which the decline in sensitivity is only at higher spatial frequencies for luminance varying stimuli.26,27 An analysis of these results in terms of optical factors could be rejected, while the results are consistent with age related changes in scotopic neural pathways.. The bottom panel of figure 3 shows the highest spatial frequency (the CSF cut-off frequency) that can be detected under scotopic conditions as a function of age. The axis on the ...
Background-While age-associated changes in LV diastolic function are well-recognized, limited data exist characterizing measures of diastolic function in older adults, including both reference ranges reflecting the older adult population and prognostically relevant values for incident HF, as well as their associations with circulating biomarkers of heart failure (HF) risk.. Methods-Among 5,801 elderly participants in the Atherosclerosis Risk in Communities (ARIC) study (age range 67-90, mean age 76 ± 5, 42% male, 21% black), we determined the continuous association of diastolic measures (TDI e, E/e, and left atrial size) with concomitant NT-proBNP and subsequent HF hospitalization or death. We also determined sex-specific 10th and 90th percentile limits for these measures using quantile regression in 401 participants free of prevalent cardiovascular disease and risk factors. Results-Each measure of diastolic function was robustly associated with NT-proBNP and incident HF or death. ARIC-based ...
Animals have evolved multiple mechanisms to protect themselves from the cumulative effects of age-related cellular damage. Here, we reveal an unexpected link between the TNF (tumour necrosis factor) inflammatory pathway, triggered by the metalloprotease ADAM17/TACE, and a lipid droplet (LD)-mediated mechanism of protecting retinal cells from age-related degeneration. Loss of ADAM17, TNF and the TNF receptor Grindelwald in pigmented glial cells of the Drosophila retina leads to age-related degeneration of both glia and neurons, preceded by an abnormal accumulation of glial LDs. We show that the glial LDs initially buffer the cells against damage caused by glial and neuronally generated reactive oxygen species (ROS), but that in later life the LDs dissipate, leading to the release of toxic peroxidated lipids. Finally, we demonstrate the existence of a conserved pathway in human iPS-derived microglia-like cells, which are central players in neurodegeneration. Overall, we have discovered a pathway mediated
Inadequate pancreatic β cell function underlies type 1 and type 2 diabetes mellitus. Strategies to expand functional cells have focused on discovering and controlling mechanisms that limit the proliferation of human β cells. Here, we developed an engraftment strategy to examine age-associated human islet cell replication competence and reveal mechanisms underlying age-dependent decline of β cell proliferation in human islets. We found that exendin-4 (Ex-4), an agonist of the glucagon-like peptide 1 receptor (GLP-1R), stimulates human β cell proliferation in juvenile but not adult islets. This age-dependent responsiveness does not reflect loss of GLP-1R signaling in adult islets, since Ex-4 treatment stimulated insulin secretion by both juvenile and adult human β cells. We show that the mitogenic effect of Ex-4 requires calcineurin/nuclear factor of activated T cells (NFAT) signaling. In juvenile islets, Ex-4 induced expression of calcineurin/NFAT signaling components as well as target genes ...
Inadequate pancreatic β cell function underlies type 1 and type 2 diabetes mellitus. Strategies to expand functional cells have focused on discovering and controlling mechanisms that limit the proliferation of human β cells. Here, we developed an engraftment strategy to examine age-associated human islet cell replication competence and reveal mechanisms underlying age-dependent decline of β cell proliferation in human islets. We found that exendin-4 (Ex-4), an agonist of the glucagon-like peptide 1 receptor (GLP-1R), stimulates human β cell proliferation in juvenile but not adult islets. This age-dependent responsiveness does not reflect loss of GLP-1R signaling in adult islets, since Ex-4 treatment stimulated insulin secretion by both juvenile and adult human β cells. We show that the mitogenic effect of Ex-4 requires calcineurin/nuclear factor of activated T cells (NFAT) signaling. In juvenile islets, Ex-4 induced expression of calcineurin/NFAT signaling components as well as target genes ...
Inadequate pancreatic β cell function underlies type 1 and type 2 diabetes mellitus. Strategies to expand functional cells have focused on discovering and controlling mechanisms that limit the proliferation of human β cells. Here, we developed an engraftment strategy to examine age-associated human islet cell replication competence and reveal mechanisms underlying age-dependent decline of β cell proliferation in human islets. We found that exendin-4 (Ex-4), an agonist of the glucagon-like peptide 1 receptor (GLP-1R), stimulates human β cell proliferation in juvenile but not adult islets. This age-dependent responsiveness does not reflect loss of GLP-1R signaling in adult islets, since Ex-4 treatment stimulated insulin secretion by both juvenile and adult human β cells. We show that the mitogenic effect of Ex-4 requires calcineurin/nuclear factor of activated T cells (NFAT) signaling. In juvenile islets, Ex-4 induced expression of calcineurin/NFAT signaling components as well as target genes ...
Other proteins that can be both oxidized and reduced by superoxide (e.g., hemoglobin) have weak SOD-like activity. Genetic inactivation (knockout) of SOD produces deleterious phenotypes in organisms ranging from bacteria to mice and have provided important clues as to the mechanisms of toxicity of superoxide in vivo. Yeast lacking both mitochondrial and cytosolic SOD grow very poorly in air, but quite well under anaerobic conditions. Absence of cytosolic SOD causes a dramatic increase in mutagenesis and genomic instability. Mice lacking mitochondrial SOD (MnSOD) die around 21 days after birth due to neurodegeneration, cardiomyopathy, and lactic acidosis.[9] Mice lacking cytosolic SOD (CuZnSOD) are viable but suffer from multiple pathologies, including reduced lifespan, liver cancer, muscle atrophy, cataracts, thymic involution, haemolytic anemia, and a very rapid age-dependent decline in female fertility.[9] Superoxide may contribute to the pathogenesis of many diseases (the evidence is ...
Given that microglia-specific Sirt1-knockout mice display elevated IL-1β production and exhibit exacerbated memory deficits in a neurodegenerative mouse model102, we can infer that SIRT1 affects the crosstalk between neurons and microglia and that its age-induced inactivation may contribute to the pathogenesis of neurodegenerative diseases.. Linking brain ageing and systemic ageingAs described in the previous section, the brain displays a range of crucial pathophysiological changes in its con-stituent cells, structures and functions during the pro-cess of ageing. Its own ageing also affects the functions of peripheral tissues and organs through many hormones and the autonomic nervous system. In particular, the hypothalamus plays a critical part in the production of many hormones and in the regulation of the autonomic nervous system, and it is emerging that age-associated decline in hypothalamic function mediates ageing at a systemic level and ultimately affects longevity.The hypothalamus ...
TY - JOUR. T1 - DNA double-strand breaks. T2 - A potential causative factor for mammalian aging?. AU - Li, Han. AU - Mitchell, James R.. AU - Hasty, Paul. PY - 2008/7/1. Y1 - 2008/7/1. N2 - Aging is a pleiotropic and stochastic process influenced by both genetics and environment. As a result the fundamental underlying causes of aging are controversial and likely diverse. Genome maintenance and in particular the repair of DNA damage is critical to ensure longevity needed for reproduction and as a consequence imperfections or defects in maintaining the genome may contribute to aging. There are many forms of DNA damage with double-strand breaks (DSBs) being the most toxic. Here we discuss DNA DSBs as a potential causative factor for aging including factors that generate DNA DSBs, pathways that repair DNA DSBs, consequences of faulty or failed DSB repair and how these consequences may lead to age-dependent decline in fitness. At the end we compare mouse models of premature aging that are defective ...
TY - JOUR. T1 - White Blood Cell Count and Mortality in the Baltimore Longitudinal Study of Aging. AU - Ruggiero, Carmelinda. AU - Metter, E. Jeffrey. AU - Cherubini, Antonio. AU - Maggio, Marcello. AU - Sen, Ranjan. AU - Najjar, Samer S.. AU - Windham, Gwen B.. AU - Ble, Alessandro. AU - Senin, Umberto. AU - Ferrucci, Luigi. PY - 2007/5/8. Y1 - 2007/5/8. N2 - Objectives: We investigated the secular trend in white blood cell (WBC) count and the relationship between WBC count and mortality between 1958 and 2002. Background: The WBC count is a clinical marker of inflammation and a strong predictor of mortality. Limited data exist on the WBC count secular trend and the relationship between WBC and mortality. Methods: One thousand eighty-three women and 1,720 men were evaluated longitudinally in the Baltimore Longitudinal Study of Aging. Blood samples and medical information were collected at the study entry and every 2 years during follow-up visits. The WBC count and all-cause, cardiovascular, and ...
We tested the hypothesis that regular aerobic exercise reverses arterial inflammation with aging. Compared with young controls (6.3 ± 0.4 mo, n=7), old (31.3 ± 0.5 mo, n=11), male B6D2F1 cage-restricted mice demonstrated increased arterial activation of the pro-inflammatory transcription factor, nuclear factor κB (NFκB), as indicated by greater aortic phosphorylation of both the inhibitor of NFκB kinase (IKK) and the p65 subunit of NFκB (both P,0.05). Similarly, aortic expression of the pro-inflammatory cytokines interleukin-1 and -6, interferon γ and tumor necrosis factor α were greater in the old mice (all P,0.05). Macrophage and T lymphocyte abundance was unchanged with age in the aortic intima and media, but was markedly increased in the adventitia and perivascular fat tissue of old mice (all P,0.05). This pro-inflammatory arterial phenotype with aging was associated with vascular endothelial dysfunction, as reflected by impaired nitric oxide-mediated endothelium-dependent dilation ...
Large breed dogs weighing 55 - 110 lbs (6+ years of age) and giant breed dogs weighing 110+ lbs (5+ years of age).. Hills® Science Diet® Mature Adult Large Breed dog food provides precisely balanced nutrition to sustain mobility and ideal body weight. It is made with high quality ingredients making it easy to digest and ensuring optimal absorption of essential nutrients. It is enriched with L-Carnitine to help turn fat into energy and with a natural source of Glucosamine and Chondroitin Sulphate to maintain healthy joints.. ...
Previous studies have identified CMV infection as a risk factor to survival in older humans (8, 13) possibly through the induction of large expanded populations of highly differentiated CD8+ T cells (7, 14). However, it is not clear how these expanded populations of T cells exert their negative influence. The main aims of the current study were to determine how highly differentiated CMV-specific CD8+CD45RA+CD27− (EMRA) T cell populations are generated and whether they are functionally competent in older humans. These cells accumulate during aging and are also CD28− and constitute part of the increased effector cell populations defined within the immune risk phenotype found in some older individuals in the OCTA/ NONA studies (8, 13).. Human CD45RA+ T cells that are activated by TCR ligation express CD45RO (40). This also applies to CD8+ EMRA T cells that are reactivated in vitro (4, 35, 41) raising the question of how CMV-specific CD8+ EMRA T cells are generated in vivo. It has been shown ...
The GluN2B subunit of the N-methyl-d-aspartate (NMDA) receptor shows age-related declines in expression across the frontal cortex and hippocampus. This decline is strongly correlated to age-related memory declines. This study was designed to determine if increasing GluN2B subunit expression in the frontal lobe or hippocampus would improve memory in aged mice. Mice were injected bilaterally with either the GluN2B vector, containing cDNA specific for the GluN2B subunit and enhanced green fluorescent protein (eGFP); a control vector or vehicle. Spatial memory, cognitive flexibility, and associative memory were assessed using the Morris water maze. Aged mice, with increased GluN2B subunit expression, exhibited improved long-term spatial memory, comparable to young mice. However, memory was rescued on different days in the Morris water maze; early for hippocampal GluN2B subunit enrichment and later for the frontal lobe. A higher concentration of the GluN2B antagonist, Ro 25-6981, was required to ...
People presenting with persistent joint pain over the age 45 years are often given the diagnosis of Osteoarthritis (OA). Osteoarthritis presents as normal age related changes in joints. OA is not simply a process of...
200. Study Of Age Related Changes In Dentate And Edentate Mandible. Sangeeta.J.Rajani. Medical College, Vadodara, Gujarat.. 80 mandibles [40 dentulous and 40 edentulous] were examined for the position of mental foramen and angle of mandible. Radiological position of mandibular canal is studied and all findings compared with other authors. In present study -A) Dentulous mandible: position of mental foramen is. found midway between the borders of the body in 60% of the cases, in 28% cases, it is near the lower border, in 12% cases, it is near the upper border, angle of mandible found between 100 to 130 degrees.. B) Edentulous Mandible: in 84% cases, position of mental foramen is near upper border, in 13% of the cases position of mental foramen is midway between the upper and lower border, in 3% of the cases position of mental foramen is near the lower border, angle found between 120 to 140 degrees. All these observations suggest that as age advances,. in majority of the cases, mental foramen ...
Ageing imposes a barrier to somatic cell reprogramming through mechanisms that are poorly understood. Here we studied the age-associated decline in reprogramming efficiency of fibroblasts der
With the development of society, aged population is growing. Hip fracture is the most common disease for aged people. With the life being longer than before, incidence of this disease is growing. The mortality of this disease is high-- almost 10% patients will die within 1 month, about 1/3 of patients will die within 12 months. About 20%-30% aged people who have hip fracture will die within one year.. The damaged organs caused by excessive inflammatory is one of possible reasons to cause higher mortality. Therefore, the investigators imagined that if they gave medicines to patients in time to reduce the inflammatory level, the inflammatory might have less effects on organs, and the recovery could be improved.. The investigators hypothesis on the basic research: the anti-inflammatory function of non steroidal antiinflammatory drugs (NSAIDs) can inhibit the inflammatory level of elderly hip fracture, so as to improve the recovery level and reduce the complicating disease and mortality. The ...
New research, published today in Nature, reveals how increasing brain stiffness as we age causes brain stem cell dysfunction, and demonstrates new ways to reverse older stem cells to a younger, healthier state. The results have far reaching implications for how we understand the ageing process, and how we might develop much-needed treatments for age-related brain diseases.. As our bodies age, muscles and joints can become stiff, making everyday movements more difficult. This study shows the same is true in our brains, and that age-related brain stiffening has a significant impact on the function of brain stem cells.. A multi-disciplinary research team, based at the Wellcome-MRC Cambridge Stem Cell Institute (University of Cambridge), studied young and old rat brains to understand the impact of age-related brain stiffening on the function of oligodendrocyte progenitor cells (OPCs).. These cells are a type of brain stem cell important for maintaining normal brain function, and for the regeneration ...
Researchers from Mayo Clinic report that targeting senescent cells-cells that are associated with aging and age-related disease-can reverse bone loss and promote an increase in bone mass and strength. Their work with mouse models may open a path to the development of new and more effective treatments for osteoporosis, a condition affecting some 44 million Americans over age 50. Sundeep Khosla, MD, director of the Aging Bone and Muscle program at Mayo Clinics Robert and Arlene Kogod Center on Aging, commented, The novelty of this work for the bone field lies in the fact that, rather than targeting a bone-specific pathway, as is the case for all current treatments for osteoporosis, we targeted a fundamental aging process that has the potential to improve not only bone mass, but also alleviate other age-related conditions as a group. The findings are published online in Nature Medicine.. Working with mice who were equivalent in age to 70+ year old humans, the researchers found that 3 different ...
I have been following this young group of dancers and creators for the last few years. Vishnu Arunasalam, a health practitioner but a dancer, singer and creator at heart, wears the responsibility of creative direction for this young group of Western Sydney artists and dancers with pride. Forging forward between long hours of demanding health covid test camps at work and still keeping his creative energies harnessed and motivated has been his major fete this year. Hence the small residency support they received from Critical Path came at a critical time in their creative journey - exploring dance within the frame of a camera. Vishnu says For many of us in Agal, the pandemic has forced us to slow down with our art-making due to several reasons such as a lack of space, isolation and lockdown restrictions which meant no gathering for creative explorations with fellow company members, funding cuts to our professional development program, lack of performance showcase opportunities and so on. For me ...
During a simple ceremony that preserves the traditions, the philosophy and the values of the Olympic spirit, the President of the International Olympic Academy Mr Michael Fysentzidis declared the opening of the works of the 57th International Session for Young Participants on the hill of the Pnyx.. The Session that will be concluded on the 1st of July will attend 153 young people from 88 countries. The Sessions main subject is: Governance in Sport and the Olympic Movement and its special subject: Ethics, Education and Governance in the Olympic Movement.. The Deputy Minister of Culture and Sport Georgios Vasileiadis, the member of the parliamentary group of New Democracy Anna Karamanli, the IOC Honorary Members Lambis Nikolaou and the former King of Greece Constantine, the Vice President of the International Olympic Academy Athanassios Strigas together with the rest of the IOA Ephoria Members, the President of the Hellenic Olympic Committee Spyros Capralos, the General Secretary of the ...
Stock Footage of Aged people play cards together at famous Temple of Heaven on Feb 20, 2017 in Beijing, China. Aging of Chinese population is a major challenge to China recently years.
The Gompertz-Makeham law states that death rate is a sum of age-independent component (Makeham term) and age-dependent component (Gompertz function), which increases exponentially with age. The compensation law of mortality (late-life mortality convergence) states that the relative differences in death rates between different populations of the same biological species are decreasing with age, because the higher initial death rates are compensated by lower pace of their increase with age. The Late-life mortality deceleration law states that death rates stop increasing exponentially at advanced ages and level-off to the late-life mortality plateau. An immediate consequence from this observation is that there is no fixed upper limit to human longevity - there is no special fixed number, which separates possible and impossible values of lifespan. This challenges the common belief[1][2] in existence of a fixed maximal human life span. Biodemographic studies found that even genetically identical ...
Aging has been associated with the accumulation of damages in molecules and organelles in cells, particularly mitochondria. The rate of damage accumulation is closely tied to the turnover of the affected cellular components. Perturbing mitochondrial turnover has been shown to significantly affect th …
Previously, we found marked age-dependent changes in the occurrence of the C4B*Q0 allele in healthy people: the frequency of the allele was found to drop with increasing age. Frequency values were 0.161 and 0.054 in 252 healthy subjects aged ,45 years and 485 healthy subjects aged ,60 years, respectively.18 To explain this dramatic change, we have assumed that the C4B*Q0 allele is a negative selection factor for health and survival: carriers of C4B*Q0 are continuously selected from the healthy population because of their increased susceptibility for life-threatening and/or chronic diseases.18 The leading cause of death in Hungary, as in many other parts of the developed world, is CHD and its consequence, myocardial infarction. In populations of Hungary and Iceland, we have obtained much evidence indicating that the morbidity in CHD and myocardial infarction is significantly higher in C4B*Q0 carriers than in noncarriers.9 10 The difference in frequency between these patients and age-matched ...
This study examined relations of PP and PWV, markers of arterial stiffness, with longitudinal assessment of cognitive function in stroke- and dementia-free persons using a fairly extensive range of neuropsychological tests. Results revealed that, as individuals grew older, those with accelerated increases in PP or higher PWV at baseline showed a performance decline on tests of verbal and nonverbal learning and memory and a cognitive screening measure weighted for memory and concentration. Those with increasing levels of PP also displayed a longitudinal decline on a test of concentration that taps working memory, and those with higher baseline PWV showed decline in delayed verbal recall. In addition, an interaction of PP and sex on delayed verbal memory revealed that, across all of the testing sessions, men scored lower than women, but this effect was a bit more pronounced at higher levels of PP. Tests of simple attention, perceptuo-motor speed, executive functions, and language were not impacted ...
Raging hormones are often blamed for the sometimes erratic behavior of teenagers; yet, hormonal fluctuations arent saddled with the stigma they deserve for altering biological behaviors in older adults. An example of such a phenomenon is the senescent immune system, which responds poorly to new stimuli. This failure may stem in part from the shrinking of the thymus that begins by puberty (thymic involution). However, in contrast to what one might intuit, this age-related muting of the immune response does not improve tolerance induction and reduce rejection after cell or organ transplantation. Instead, an active immune system is required to promote tolerance to novel antigens. Zhao et al. now show that modifying hormone concentrations can restore the induction of transplant tolerance in aged mice.. The authors found that after the age of 12 months, mice became resistant to tolerance induction for cardiac transplants. However, surgical castration led to long-term graft acceptance and restoration ...
In one of the largest studies of its kind, age-related changes to speech-evoked auditory brainstem responses (ABR) were compared between musicians and non-musicians, using a dataset of more than 700 participants (250-plus musicians), spanning in age from 3 to 73. The outcomes of this cross-sectional study suggest that music-making does not, in fact, speed up or slow down the major milestones of ABR development. Instead, making music routinely may affect how age-dependent changes are expressed in the auditory system, potentially allowing the auditory system to take greater advantage of sensitive periods to promote resilient, more youthful-looking auditory processing later in life ...
The aim of this cross-sectional study was to analyze the typical aging process in adults with Down syndrome, focusing on its variability. The sample comprised 120 adults with Down syndrome who were free of dementia. Ages ranged from 20 to 69 years. Each participant was assessed on cognitive functioning and social adaptation, and was checked for the presence of psychopathological disorders. Results revealed an age-related deterioration in both cognitive and social adaptation skills, the extent of this decline depending on the dimension under scrutiny, and interindividual variability in aging profiles.
In your mind, you might be picturing cholesterol as an order of fries, honey bacon and other oily foods that harm our body. However, studies discovered that cholesterol properties give valuable benefits to our skincare!. A healthy skin depends on lipids which play a crucial role in maintaining a healthy skin barrier and preventing trans-epidermal water loss. Skin aging is caused by a decline in lipid production (especially cholesterol). This includes functional changes such as age-related deterioration of the skin barrier leading to skin dehydration, reduced protection, and repair against daily aggressors (pollution, UV, stress). The clinical implications of depleted lipids include large pores, uneven texture, deep wrinkles and loss of radiance.. Cholesterol can be found in all cells of your body and is essential to produce hormones and vitamin D. But the cholesterol in skin differs from the one found in the blood. It provides skin structure and elasticity. According to Lipidomic studies, they ...
In the article, co-directed by Coral Sanfeliu, researcher at the Higher Council for Scientific Research at the Biomedical Research Institute of Barcelona (IIBB), the scientific team has evaluated whether long-term sports training can delay the onset of physiological memory loss and promote its maintenance as aging occurs. The research has involved athletes from the clubs of veterans XV Matusalem Rugby Sant Cugat and Kings Pebrots, and also of the Club de Rugby Barcelona.. Mª Carmen Gómez Cabrera, professor of the Department of Physiology of the University of Valencia and INCLIVA researcher, explains that for the study a total of eighty-six healthy men of ages between 17 and 68 years were recruited. Among them we include a middle-aged group of rugby players with an average of between 15 and 35 years of sports practice.. The work analyses biomarkers. As the research concludes, physical training allows to modulate various factors involved in molecular communication, through factors released in ...
TY - JOUR. T1 - Dynamic regulation of vascular myosin light chain (MYL9) with injury and aging. AU - Shehadeh, Lina A.. AU - Webster, Keith A.. AU - Hare, Joshua M.. AU - Vazquez-Padron, Roberto I.. N1 - Copyright: Copyright 2012 Elsevier B.V., All rights reserved.. PY - 2011/10/7. Y1 - 2011/10/7. N2 - Background: Aging-associated changes in the cardiovascular system increase the risk for disease development and lead to profound alterations in vascular reactivity and stiffness. Elucidating the molecular response of arteries to injury and age will help understand the exaggerated remodeling of aging vessels. Methodology/Principal Findings: We studied the gene expression profile in a model of mechanical vascular injury in the iliac artery of aging (22 months old) and young rats (4 months old). We investigated aging-related variations in gene expression at 30 min, 3 d and 7 d post injury. We found that the Myosin Light Chain gene (MYL9) was the only gene differentially expressed in the aged versus ...