Two previous studies have observed natural transformation in A. actinomycetemcomitans. Tøonjum et al. (30) used a broth-based method and identified two naturally competent strains among five study strains of A. actinomycetemcomitans with transformation frequencies of 1.3 × 10−3 and 6.2 × 10−5. In our present study, we identified a single highly competent A. actinomycetemcomitans strain among 17 strains examined. Although this is an apparently lower prevalence of naturally competent A. actinomycetemcomitans strains than was reported by Tøonjum et al., the transformation frequencies reported in this study were similar.. Sato et al. (23) generated transposon insertional mutants from the A. actinomycetemcomitans strain Y4 by natural transformation. The transformation was performed with broth-cultured exponential-phase A. actinomycetemcomitans cells. However, strain Y4 was not transformable in our study. While the loss of competence is sometimes a consequence of laboratory propagation, it is ...

Patients with localized juvenile periodontitis (LJP) often have high titers of antibody reactive with the serotype-specific immunodominant carbohydrate antigen of Actinobacillus actinomycetemcomitans serotype b. The vast majority of this A. actinomycetemcomitans serotype b-specific antibody is immunoglobulin G2 (IgG2). The present study was undertaken to determine whether the overall total levels of IgG2 in the sera of LJP patients are elevated. LJP patients and nonperiodontitis (NP) controls matched for age, race (black and white), and gender were studied. Additional controls included patients with adult periodontitis (AP) and patients similar in age to LJP patients but with the more-severe, generalized form of early-onset periodontitis (SP). Sera from over 700 periodontally characterized subjects were examined by using radial immunodiffusion to quantitate IgG2 as well as IgG1, -3, and -4, which were included for comparison. Serum IgG2 levels increased with age, and this was most dramatic ...

Periodontitis is mankinds most common chronic inflammatory disease. One severe form of periodontitis is localized aggressive periodontitis (LAP), a condition to which individuals of African origin demonstrate an increased susceptibility. The main causative organism of this disease is Actinobacillus …

Aggregatibacter (Actinobacillus) actinomycetemcomitans is a bacterium mainly associated with aggressive forms of periodontitis. Among its virulence factors, a leukotoxin is suggested to play an important role in the pathogenicity. Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. Leukotoxin selectively kills human leukocytes and can disrupt the local defense mechanisms. Previous studies examining the role of the leukotoxin in host-parasite interactions have mainly focused on polymorphonuclear leukocytes (PMNs). In the inflamed periodontium, macrophages play a significant role in the regulation of the inflammatory reactions and the tissue breakdown and remodeling.. Thus, the aim of this dissertation was to investigate death mechanisms of human macrophages exposed to leukotoxin.. Human lymphocytes, PMNs, and monocytes/macrophages isolated from venous blood were exposed to purified leukotoxin or live A. actinomycetemcomitans ...

Aggregatibacter actinomycetemcomitans (A.a) serotypes may add some important information of the pathogenetic background of periodontal infections. (adsbygoogle = window.adsbygoogle ,, []).push({}); When grown in culture, these form small colonies, approximately 0.5-1.0 mm in diameter. The cytotoxin produced by, Almost all strains of A. actinomycetemcomitans have these vesicles. 2008 Apr 1;10(5):489-96. The results of the study showed that these antibodies might contribute to limit the systemic effects of the infection. Ltx B and Ltx D are involved in transporting the toxin to the surface of the cell while Ltx C post-transitionally activates the toxin. The most common bacteria involved in periodontal pathologies of young individuals and in cases of refractory adult periodontitis is Aggregatibacter actinomycetemcomitans. This genus includes Gram-negative, non-motile, facultatively anaerobic rods or coccobacilli that were previously known as Haemophilus. The active subunit, CdtB, exhibits DNase I ...

Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:

Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:

Structures of the antigenic O-polysaccharides of lipopolysaccharides produced by Actinobacillus actinomycetemcomitans serotypes a, c, d and e

The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, ...

Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive periodontitis (LAP) and other systemic inf...

0165] (1) Wilson M, Henderson, B. Virulence factors of A. actinomycetemcomitans revenant to the pathogenesis of inflammatory periodontal diseases. FEMS 1995:17:365-379. [0166] (2) Nowotny A, Behling U H, Hammond B, et al. Release of toxic microvesicles by Actinobacillus actinomycetemcomitans. Infect Immun 1982:37:151-154. [0167] (3) Kaplan J B, Perry M B, MacLean L L, Furgang D, Wilson M E, Fine D H. Structural and genetic analyses of 0 polysaccharide from Actinobacillus actinomycetemcomitans serotype f. Infect Immun 2001:69:5375-5384. [0168] (4) Rosan B, Slots J, Lamont R J, Listgarten M A, Nelson G M. Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol 1988:3:58-63. [0169] (5) Inouye T, Ohta H, Kokeguchi S, Fukui K, Kato K. Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans. FEMS microbiology letters 1990: 57: 13-17. [0170] (6) Kaplan J B, Schreiner H C, Furgang D, Fine D H. Population structure and genetic diversity of Actinobacillus ...

TY - JOUR. T1 - Infection by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, and antibody responses at different ages in humans.. AU - Nakagawa, S.. AU - Machida, Y.. AU - Nakagawa, Taneaki. AU - Fujii, H.. AU - Yamada, S.. AU - Takazoe, I.. AU - Okuda, K.. PY - 1994/1. Y1 - 1994/1. N2 - This study examined the serum IgG and IgM responses against Porphyromonas gingivalis and 3 serotypes of Actinobacillus actinomycetemcomitans, and the correlations of these responses with age and homologous infection. A total of 90 individuals were included in this study: 40 subjects with gingivitis, 40 periodontally healthy subjects, and 10 adult periodontitis subjects. The subjects in the gingivitis and periodontally healthy groups were divided into 4 stages based on their physiological age: early childhood, school age, puberty, and adult. In the gingivitis group, there was a positive correlation between increase in age and increase in serum IgG antibody levels against P. gingivalis until ...

TY - JOUR. T1 - Cell surface-associated enolase in Actinobacillus actinomycetemcomitans. AU - Hara, Hiroaki. AU - Ohta, Hiroyuki. AU - Inoue, Tetsuyoshi. AU - Ohashi, Toshio. AU - Takashiba, Shogo. AU - Murayama, Yoji. AU - Fukui, Kazuhiro. PY - 2000. Y1 - 2000. N2 - Cell surface-associated materials of Actinobacillus actinomycetemcomitans were extracted by a short incubation of the cell suspension in a Tris-buffered saline in the presence and absence of a restriction enzyme, EcoRI. The supernatants (which we termed EcoRI extract and surface extract, respectively) contained a number of extracellularly released proteins. Of these proteins, four major proteins were identified by N-terminal sequencing to be the 34 and 39 kDa outer membrane proteins, the GroEL-like protein, and a 47 kDa protein homologous to Haemophilus influenzae enolase. Enolase activity was found in the extracts and its relative amount of activity in the EcoRI extract from a culture of the mid-exponential growth phase was ...

Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid-structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, and substantially lower monospecies biofilm formation relative to the wild type. Furthermore, the D7S hns mutant exhibited significantly reduced growth within a seven-species oral biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species regardless of being co-cultivated with D7S hnsor its parental strain. Proteomics analysis ...

Many gram-positive and gram-negative bacterial species express LuxS and secrete and respond in various ways to a signal that is related to autoinducer 2 of V. harveyi (4, 7, 9, 10, 14, 16, 18, 19, 20, 21, 30, 31, 41). However, few of these organisms possess the dedicated two-component circuit that mediates the cell density-dependent response of V. harveyi to AI-2. Our previous studies showed that AI-2 secreted by the oral pathogen A. actinomycetemcomitans was capable of inducing V. harveyi bioluminescence (10) and also influenced aerobic growth of A. actinomycetemcomitans under iron-limiting conditions (9) These studies also suggested that AI-2-mediated regulation of iron uptake and storage genes contributed to optimal growth of A. actinomycetemcomitans under iron limitation. However, the mechanism by which A. actinomycetemcomitans responds to AI-2 is not fully understood. Analysis of the complete genome sequence of A. actinomycetemcomitans HK1651 (26; www.oralgen.lanl.gov ) indicates that this ...

Adolescent; Aggregatibacter actinomycetemcomitans; Bacterial Toxins; Cohort Studies; Cross-Sectional Studies; DNA, Bacterial; Disease Progression; Ghana; Humans; Pasteurellaceae Infections; Periodontal Diseases ...

Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4 T Cells into IFNγ IL10 Producing Tbet Th1 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

ID C9R3L9_AGGAD Unreviewed; 404 AA. AC C9R3L9; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 25-OCT-2017, entry version 39. DE SubName: Full=Molybdopterin molybdenumtransferase {ECO:0000313,EMBL:ACX82405.1}; GN OrderedLocusNames=D11S_1018 {ECO:0000313,EMBL:ACX82405.1}; OS Aggregatibacter actinomycetemcomitans serotype C (strain D11S-1) OS (Actinobacillus actinomycetemcomitans). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Aggregatibacter. OX NCBI_TaxID=668336 {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569}; RN [1] {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=D11S-1 {ECO:0000313,EMBL:ACX82405.1, RC ECO:0000313,Proteomes:UP000002569}; RX PubMed=19820097; DOI=10.1128/JB.01203-09; RA Chen C., Kittichotirat W., Si Y., Bumgarner R.; RT Genome sequence of Aggregatibacter actinomycetemcomitans serotype c RT strain D11S-1.; ...

Monoclonal antibody against Aggregatibacter actinomycetemcomitans serotype b expressed by for use in Western Blot against gram-negative bacteria

A major etiologic agent of human periodontal disease. This strain was recovered from the subgingival plaque of a patient diagnosed with generalized aggressive periodontitis ...

TSBV agar is an enriched selective medium used for the isolation of Aggregatibacter actinomycetemcomitans from clinical specimens. The nutritive base consists of casein, soy peptone, and yeast extract. Sodium chloride is added to maintain osmotic equilibrium of the media. Serum is included to enhance the recovery of Aggregatibacter actinomycetemcomitans. The selective agents include bacitracin and vancomycin at concentrations that inhibit most gram-positive and gram-negative anaerobes. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.. ...

Background: This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMC) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. The supernatants and cell wall extracts (CWEs) of eight A. actinomyc...

The focus of my research is the identification and characterization of bacterial proteins that are required for tissue colonization, the initiation of any infection. My research interests are concentrated on the membrane proteins and surface structures of the Gram-negative bacterium Aggregatibacter actinomycetemcomitans. A. actinomycetemcomitans is typically associated with Periodontitis, an inflammatory disease of the tissues surrounding and supporting the teeth, which left untreated results in tooth loss. This bacterium is also associated with numerous non-oral diseases including but not restricted to infective endocarditis and atherosclerosis, which suggest that the periodontal pocket is a potential source and reservoir of these diseases. The emphasis of my research is based on two genes and the associated proteins identified in my laboratory. We have identified and characterized a unique collagen adhesin expressed by A. actinomycetemcomitans. The extracellular matrix protein adhesin A (EmaA) ...

A Periodontite Agressiva (PA), que atualmente pertence ao grupo das Periodontites est gios 3 e 4, distingue-se dos demais tipos de doen a periodontal por seu in cio precoce, agrega o familiar dos casos e por afetar pacientes sistemicamente saud veis. Al m disso, pode ser subclassificada em duas formas, localizada (PAL) e generalizada (PAG), em fun o de sua extens o. Muitas vezes, os dep sitos de biofilme bacteriano s o desproporcionais quantidade de destrui o ssea e perda de inser o que o paciente apresenta, independente da subclassifica o. O microrganismo mais relacionado etiopatog nese da doen a o Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), incluindo os seus principais sorotipos a, b e c, amplamente estudados. Associado a estas condi es, A. actinomycetemcomitans apresenta alguns fatores de virul ncia como uma leucotoxina, principalmente ligada ao sorotipo b - clone JP2 (que altamente leucot xico) e prote nas de membrana externa (OMPs), especialmente Omp29. A resposta de ...

Johns Hopkins Medicine (JHM). December 14, 2016. Investigators at Johns Hopkins report they have new evidence that a bacterium known to cause chronic inflammatory gum infections also triggers the inflammatory autoimmune response characteristic of chronic, joint-destroying rheumatoid arthritis (RA). The new findings have important implications for prevention and treatment of RA, say the researchers.. In a report on the work, published in the Dec. 14 edition of the journal Science Translational Medicine, the investigators say the common denominator they identified in periodontal disease (gum disease) and in many people with RA is Aggregatibacter actinomycetemcomitans. An infection with A. actinomycetemcomitans appears to induce the production of citrullinated proteins, which are suspected of activating the immune system and driving the cascade of events leading to RA.. This is like putting together the last few pieces of a complicated jigsaw puzzle that has been worked on for many years, says ...

Herrera Gonzalez, David and Alonso Álvarez, Bettina and Arriba de la Fuente, Lorenzo de and Santa- Cruz Astorqui, Isabel and Serrano, Cristina and Sanz Alonso, Mariano (2014) Acute periodontal lesions. Periodontology 2000, 65 (1). pp. 149-177. ISSN 0906-6713 Mínguez, María and Pousa, Xiana and Herrera Gonzalez, David and Blasi, Andrea and Sánchez, Mari Carmen and León, Rubén and Sanz Alonso, Mariano (2014) Characterization and serotype distribution of Aggregatibacter actinomycetemcomitans isolated from a population of periodontitis patients in Spain. Archives of Oral Biology, 59 (12). pp. 1359-1367. ISSN 0003-9969 Alonso Álvarez, Bettina and Herrera Gonzalez, David and Sanz Alonso, Mariano and Iniesta Albentosa, Margarita and de Arriba de la Fuente, Lorenzo and Legido Arce, Berta and SantaCruz Astorqui, Isabel and Sánchez Pérez, Nerea and Mínguez Arnau, María (2014) Entrenamiento basado en un entorno interactivo para mejorar las competencias al final del grado. Proyecto de Innovación ...

Immunological detection of antigens of Aggregatibacter actinomycetemcomitans, P intermedia, P gingivalisusing antibodies (ELISA ...

What causes rheumatoid arthritis?. The search, by scientists around the world, has been exhaustive, and the prey surprisingly elusive - but in breakthrough Johns Hopkins research, scientists Maximilian Konig, M.D., and Felipe Andrade, M.D., Ph.D., believe they have pinpointed one culprit: a kind of bacteria that lives in the mouth and is present in gum disease.. This form of bacteria has an unpronounceable name, Aggregatibacter actinomycetemcomitans, so the scientists who have discovered the terrible and important role it might play in RA just call it by its initials: Aa. We are very excited about it, says Konig. If their hypothesis is verified by other scientists and in larger studies, the possibilities of preventing RA from developing, and of treating early RA and possibly reversing symptoms, are enormous. This work was presented at the Plenary Session of the American College of Rheumatologys Annual Scientific Meeting in November 2016, and I recently wrote about it for Leap magazine, a ...

Great news! Youre in the right place for Timken JP10049-JP10010-B bearing. By now you already know that, whatever you are looking for, youre sure to find it on AliExpress.

マーケットレポート.jp（MarketReport.jp）世界の市場調査レポートの総合販売サイトです。IT・通信産業、化学産業、エネルギー産業、自動車産業、医療産業、電子産業、食品産業など多様な産業の調査レポートを15万件以上取り扱っています。日本国内、アメリカ、中国、ヨーロッパなど地域レポートも販売しています。

お料理ブログのレシピポータルサイト。みきママさん、山本ゆりさんなど人気ブロガーが多数活躍！73万件以上の料理レシピの中から、毎日のおかず、弁当、お菓子など簡単料理を検索。さらに人気順の並び替えが無料です！

理論生物物理学ユニット グレッグ・スティーブンズ 准教授（アジャンクト） greg.stephens at oist.jp 物理学者たちは長い間、物質およびエネルギーの本質を説明できる普遍的法則を探し求めてきましたが、最近まで複雑な生物系の研究は困難でした。理論生物物理ユニットでは、生体の脳および行動に関するシンプルで統一的な原理を探索しています。実験研究者と緊密に連携しながら、ユニットのメンバーは定量的な生物測定を、統計物理学、情報理論、およびダイナミカル（動的）システムから導かれる理論的概念と結び付けています。

Investigation of the structure of lipid A from Actinobacillus actinomycetemcomitans strain Y 4 and human clinical isolate PO 1021-7

This study evaluated the effect of oral lactobacilli on the cytotoxicity and cytokine release from peripheral blood mononuclear cells (PBMCs) when exposed to Aggregatibacter actinomycetemcomitans subtypes in vitro. The supernatants and cell wall extracts (CWEs) of eight A. actinomycetemcomitans strains, representing different subtypes, and three Lactobacillus strains were used. The PBMCs from six blood donors were exposed to supernatants and CWEs of A. actinomycetemcomitans or Lactobacillus strains alone or combinations and untreated cells as control. The cytotoxicity was determined by trypan blue exclusion method and IL-1β secretion by ELISA. TNF-α, IL-6, and IL-8 secretions were measured using Bioplex Multiplex Immunoassay. Supernatants or CWEs from all bacterial strains showed cytotoxicity and IL-1β secretion and the subtypes of A. actinomycetemcomitans showed generally a significantly higher effect on PBMCs than that of the Lactobacillus strains. Two highly toxic A. actinomycetemcomitans strains

The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark and the ATCC catalog marks are trademarks of ATCC. Microbiologics, Inc. is licensed to use these trademarks and to sell products derived from ATCC® cultures. Look for the ATCC Licensed Derivative © Emblem for products derived from ATCC© cultures. ...

Sockett and Lambert, 2004. Balos are ubiquitous in nature and have been isolated from diverse terrestrial and aquatic habitats, from biofilms as well as from animal feces

Fingerprint Dive into the research topics of Isolation and characterization of the dnaKJ operon from Actinobacillus actinomycetemcomitans. Together they form a unique fingerprint. ...

Pathogenic bacteria produce numerous virulence factors, including protein toxins, to enhance their growth and survival within the host. Our lab seeks to understand the mechanisms of bacterial toxin delivery to identify and exploit therapeutic targets. In this project, we have focused on a member of the repeats-in-toxin (RTX) family of proteins, which are secreted by many Gram negative bacteria, including Bordetella pertussis, Escherichia coli, and Aggregatibacter actinomycetemcomitans. The leukotoxin (LtxA) secreted by A. actinomycetemcomitans specifically kills human white blood cells to disrupt the host immune response and therefore plays a key role in bacterial colonization of the host. The cell type specificity of LtxA arises from its reported targeting of the lymphocyte function-associated antigen-1 (LFA-1) integrin, which is only expressed by human white blood cells. In addition, we recently showed that LtxA binds with a strong affinity to cholesterol, and inhibition of this binding to ...

Aggregatibacter actinomycetemcomitans (Aa) expresses a 64-kDa GroEL protein belonging to the heat shock family of proteins. This protein has been shown to influence human host cells, but the apoptotic capacity of the GroEL protein regarding T cells is not yet known. The purpose of this study was to investigate the ability of A. actinomycetemcomitans GroEL (AaGroEL) protein to induce human peripheral blood T-cell apoptosis. Endogenous, purified AaGroEL protein was used as an antigen. In AaGroEL-treated T cells, the data indicated that phosphatidylserine exposure, an early apoptotic event, was dose- and time-dependent. The AaGroEL-treated T cells were also positive for active caspase-3 in a dose-dependent manner. The rate of AaGroEL-induced apoptosis was suppressed by the addition of the general caspase inhibitor Z-VAD-FMK. Furthermore, cleaved caspase-8 bands (40/36 kDa and 23 kDa) were identified in cells responding to AaGroEL. DNA fragmentation was also detected in the AaGroEL-treated T cells. ...

We have observed that autoantibodies reactive with important phospholipid antigens are present in the sera and gingival crevicular fluids of patients with periodontitis. The antibodies are likely induced by commonly occuring plaque bacteria. Phosphorylcholine (PC) is a prevalent antigen found on 30-40% of plaque bacteria, and patients with periodontal attachment loss have significantly higher concentrations of IgG anti-PC than do individuals without attachment loss. Data indicate that these antibodies bind to and opsonize oral microorganisms such as Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) and further that they react with and opsonize modified low-density lipoproteins (LDL). Anti-β2-glycoprotein 1-dependent anti-cardiolipin (anti-CL), another antibody of interest, is found at elevated levels in 15-20% of patients with generalized periodontitis. In patients with systemic lupus erythemathosis (SLE) or the antiphospholipid syndrome (APS) elevated levels of these antibodies ...

Objectives: To investigate the short-term effects of nonsurgical therapy (scaling and root planing, SRP) on the subgingival microbiota in chronic (CP) and aggressive (AP) perio dontal disease. Method and Materials: Ninety-seven CP and AP subjects underwent full-mouth SRP on 2 consecutive days. AP patients were randomly assigned to either receive systemic metronidazole plus amoxicillin (AP+AB) or were treated mechanically alone (AP). Pathogens were identified with 16S rRNA oligodeoxynucleotide probes and dot-blot hybridization before and at days 2, 3, 4, 7, 10, and 21 of healing. CP subjects were treated by scaling and root planing along with placebo tablets. Results: Initially, AP cell counts were 69.9- (Porphyromonas gingivalis), 10.2- (Aggregatibacter actinomycetemcomitans), 5.7- (Tannerella forsythia), and 3.3-fold (Prevotella intermedia) enhanced compared to CP cell counts. Following SRP, immediate elimination occurred in single individuals of all three treatment groups at day 2. After SRP ...

Accepted name: dTDP-4-dehydro-6-deoxyglucose reductase. Reaction: dTDP-α-D-fucopyranose + NAD(P)+ = dTDP-4-dehydro-6-deoxy-α-D-glucose + NAD(P)H + H+. For diagram of reaction click here.. Glossary: dTDP-4-dehydro-6-deoxy-α-D-glucose = dTDP-6-deoxy-α-D-xylo-hexopyranos-4-ulose = thymidine 5-[3-(6-deoxy-D-xylo-hexopyranosyl-4-ulose) diphosphate]. Other name(s): dTDP-4-keto-6-deoxyglucose reductase; dTDP-D-fucose:NADP+ oxidoreductase; Fcf1; dTDP-6-deoxy-D-xylo-hex-4-ulopyranose reductase. Systematic name: dTDP-α-D-fucopyranose:NAD(P)+ oxidoreductase. Comments: The enzymes from the Gram-negative bacteria Aggregatibacter actinomycetemcomitans and Escherichia coli O52 are involved in activation of fucose for incorporation into capsular polysaccharide O-antigens [1,3]. The enzyme from the Gram-positive bacterium Geobacillus tepidamans is involved in activation of fucose for incorporation into the organisms S-layer [2].The enzyme from Escherichia coli O52 has a higher catalytic efficiency with ...

Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil ...

The researchers performed full-mouth examinations to assess participants periodontal conditions. Saliva and dental plaque samples were collected to evaluate colonization by several of pathogens - P. gingivalis, T. denticola, T. forsythia, and A. actinomycetemcomitans - and to characterize oral microbial diversity.. Compared with the control group, patients with precancerous lesions experienced higher prevalence of bleeding when probed (31.5 percent versus 22.4 percent), higher levels of two pathogens (T. denticola and A. actinomycetemcomitans), and less bacterial diversity in their saliva.. A further analysis, which took into account sociodemographic factors, oral health behaviors, and periodontal assessments, revealed additional predictors of precancerous lesions: elevated colonization of three pathogens (T. forsythia, T. denticola, and A. actinomycetemcomitans), decreased bacterial diversity in dental plaque, and not flossing regularly.. The researchers concluded that the colonization of ...

The present large-scale, general population-based data suggest that distinct species have a different carriage profile, depending on variables such as age group, educational level, smoking history, and periodontal status. In general, the carriage of periodontal pathogens proved to be common, and only 12% of the 1,294 subjects were free of all six target bacteria examined. More than half of the population had T. forsythensis, one-third T. denticola, P. gingivalis or C. rectus, and one-fifth P. intermedia or A. actinomycetemcomitans. Logistic regression models revealed different variables to be associated with distinct species in dentate subjects.. Periodontitis-associated organisms colonize not only subgingival sites, but also supragingival sites (20, 35), and appear in saliva (5, 7, 8, 15, 30, 32). Stimulation by masticating a piece of paraffin looses still-attached microorganisms or clumps of microorganisms from oral biofilms into salivary sediment. According to Umeda et al. (30), whole saliva ...

IDCore 30 rev B is a highly secured smartcard platform compliant with Javacard 2.2.2, Global Platform 2.1.1 & 2.2 Amendment D standards, designed to operate with Infineon SLE78 chip family. The library implements TDEA, AES, AES-CMAC, SHA1-224-256-384-512, RSA, RSA CRT, ECDSA, ECC CDH, SP800-108 KDF and SP800-90A DRBG ...

The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures. In a second proof-of-principle phase, we found 18 (P. gingivalis, T. forsythia and T. denticola) of the 120 in vitro compounds in the saliva from a periodontitis patient with confirmed infection with P. gingivalis, T. forsythia and T. denticola with enhanced ion intensity compared to two healthy controls. In conclusion, ...

THE EFFECT OF CURCUMA XANTHORRHIZA ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)

The cytolethal distending toxins (CDTs) are a family of exotoxins produced by a wide range of Gram-negative bacteria. They are known for causing genotoxic stress to the cell, resulting in growth arrest and eventually apoptotic cell death. Nevertheless, there is evidence that CDTs can also perturb the innate immune responses, by regulating inflammatory cytokine production and molecular mediators of bone remodeling in various cell types. These cellular and molecular events may in turn have an effect in enhancing local inflammation in diseases where CDT-producing bacteria are involved, such as Aggregatibacter actinomycetemcomitans, Haemophilus ducreyi, Campylobacter jejuni and Helicobacter hepaticus. One special example is the induction of pathological bone destruction in periodontitis. The opportunistic oral pathogen Aggregatibatcer actinoycemetemcomitans, which is involved in the aggressive form of the disease, can regulate the molecular mechanisms of bone remodeling in a manner that favors bone

Obradović, Davor, et al. A Cytolethal Distending Toxin Variant from Aggregatibacter actinomycetemcomitans with an Aberrant CdtB That Lacks the Conserved Catalytic Histidine 160. PloS one 11.7 (2016): e0159231.. Hemp, James, et al. Draft Genome Sequence of Ornatilinea apprima P3M-1, an Anaerobic Member of the Chloroflexi Class Anaerolineae. Genome Announcements 3.6 (2015): e01353-15.. Ward, Lewis M., et al. Draft Genome Sequence of Leptolinea tardivitalis YMTK-2, a Mesophilic Anaerobe from the Chloroflexi Class Anaerolineae. Genome Announcements 3.6 (2015): e01356-15.. Hemp, James, et al. Draft Genome Sequence of Levilinea saccharolytica KIBI-1, a Member of the Chloroflexi Class Anaerolineae. Genome Announcements 3.6 (2015): e01357-15.. Hemp, James, et al. Draft Genome Sequence of Ardenticatena maritima 110S, a Thermophilic Nitrate-and Iron-Reducing Member of the Chloroflexi Class Ardenticatenia. Genome Announcements 3.6 (2015): e01347-15.. Pace, Laura A., et al. Draft Genome of ...

Materials and Methods: The present study was designed as a prospective stratified randomized controlled clinical trial. Patients were scheduled to receive two implants with different types of abutments in the posterior mandible. Three months after implant placement, titanium and zirconium abutments were connected. Five weeks after abutment connections, the abutments were removed, probing depth measurements were recorded, and gingival biopsy samples were obtained. Abutments and biopsy specimens were analyzed by reverse-transcriptase polymerase chain reaction to compare the DNA copy numbers of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and total bacteria. The surface free energy of the abutments was calculated by sesile water drop before replacement ...

Teeth/gums (dental generel anaesthsia, mineralization disturbances), Infection (Actinobacillus actinomycetemcomitans, oral pathogens, population genetics of bacteria, virulence factors), Infection (antibiotics), Molecular medicine, Odontology, Teeth/gums (deveopmental disturbances of dental hard tissues, mineralization disturbances). ...

Periodontal Disease or Gum Disease is a common condition affecting about 47% of the population and has been present for millennia. Early humans also suffered from this debilitating condition.. There are usually no symptoms like pain despite the fact that the bone and ligaments holding teeth are slowly melting or literally rotting away as part of inflammation.. There are a few known disease causing bacteria that are present in all mouths such as P gingivalis, P Intermedia, and A actinomycetemcomitans. They are responsible for causing Periodontitis in susceptible individuals. Susceptibility to Periodontal Disease is largely genetically determined. In other words, if your parents, grandparents or the family in the ancestral line had Periodontal Disease, you are more likely to get the disease. There are also modifying conditions including habits, emotional stress, medications and diseases which affect Periodontal disease, for example, diet, oral hygiene, smoking, medications such as steroids or ...

開站以來我們陸續推出了MP150和JC-3兩款後級擴大機，始終少了一個前級套件來搭配，在幾個月的時間中我們開發試裝了幾套前級，最終決定先推出JP200C真空管前級做為diyResearch的第一款前級套件。. 選擇JP200C為第一款前級的主要幾個原因，第一：以Jadis JP200架構基礎的JP200C擁有很迷人的聲音特質。第二：我們對於這個電路很熟悉，這幾年來前前後後裝了許多各種不同版本的JP200，累積了豐富的調教經驗。第三：經過長時間的調整試聽之後，此版本的JP200C非常穩定，同時訊噪比優良。以下先貼出幾張量產PCB的裝機完成品照片供大家瞧瞧。. ...