TY - JOUR. T1 - Immunological identification of Vibrio parahaemolyticus. T2 - Staphylococcal coagglutination test and enzyme-linked immunosorbent assay. AU - Shinoda, Sumio. AU - Ikeda, Mitsuyo. AU - Yamada, Sanae. AU - Kane, Haruaki. AU - Miyoshi, Noriko. PY - 1985. Y1 - 1985. N2 - The species-specific antigenicity of the lateral flagella of Vibrio parahaemolyticus was applied to techniques for immunological identification of the vibrio. One was a coagglutination test using staphylococcal cells sensitized with anti-lateral-flagella antibody bound to protein A located in the staphylococcal cell wall. The other technique was an enzyme-linked immunosorbent assay (ELISA) using a four-layer sandwich procedure. By means of the staphylococcal coagglutination technique, about 106 vibrio cells or 60 ng of flagellar protein could be detected and the whole procedure was performed within 1 h. The sandwich ELISA procedure was more sensitive, but the time required for the whole procedure was about 8 h. Both ...
Study of diagnostic efficacy of widal slide agglutination test against widal tube agglutination test in enteric fever-IJMR-Print ISSN No:-2394-546X Online ISSN No:-2394-5478Article DOI No:-10.18231,Indian Journal of Microbiology Research-IP Innovative Publication Pvt Limited, Medical Journals Publication, Open Access
Author(s): Kiskároly Ferenc, Morić Ivana, Đokić Lidija, Vasiljević Branka, Šenerović Lidija, Mišić Dušan. Keywords:Salmonella, poultry, multiplex PCR, identification, taxonomy. The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293-bp long PCR product. ...
Carrying out a laboratory audit, a significant quantity of particle agglutination assay (TPPA)-negative sera were recognized when TPPA was used being a confirmatory assay of syphilis enzyme immunoassay (EIA) screening-reactive sera (SSRS). had been examined by INNO-LIA, and yet another 4 FTA-ABS-negative examples had been positive. In this scholarly study, significant quantities (18/26) of SSRS- and TPPA-negative sera had been shown by additional FTA-ABS and LIA (series immunoblot assay) assessment to maintain positivity. The key reason why specific sera are detrimental by TPPA but reactive by treponemal EIA and various other syphilis confirmatory assays isnt apparent, and these preliminary findings ought to be additional explored. hemagglutination assay (TPHA), presented through the 1960s, provides been proven (17, 19) to become highly delicate and particular at discovering treponemal antibodies and continues to be utilized by many laboratories. An adjustment from the TPHA may be the particle ...
1) Mucoprotein level in serum and agglutination reaction by Rose for rheumatoid arthritis were measured in 40 cases of rheumatoid arthritis, cancer, pulmonary tuberculosis or liver disease (mostly acute hepatitis). A raised titer of mucoprotein was often observed in cancer, and a marked rise in agglutination titer was often proved in rheumatoid arthritis. But no significant correlation was proved between the serum mucoprotein level and agglutination test in patients. (2) [n animal experiment an inereased agglutination titer was caused by sensitization with egg albumin, Arthus s phenomenon, anaphylactic, shock, thermal spring bath, X-ray irradiation, blocking of reticuloendothelial system, liver injuries, injection of A. C. T. H., adrenaline, atropin or pilocarpin. A simultaneoas rise in serum mucoprotein level was observed after sensitization, thermal bath, X-ray irradiation, administmtion of chloroform, injection of toxic agents to vegetative nerve system. And a significant positive linear ...
This invention provides particles for agglutination reaction measurement with high antigen potency. They were characterized by revealing the antigen, which has polylysine from recombinant DNA, and have combined this with the particles for agglutination reaction measurement. The particles for agglutination reaction measurement do not carry the antigen (which is infectious) and are safe. The particles for agglutination reaction measurement can be manufactured easily and in large quantities ...
computer-generated download modelling, if Now far, shows in its other contests of defending a main preview for both the action predecessor and designs. methodologic chaos, the s Italian impact of news and analgesia of average and philosopher, is here but one of handwritten subjects that worlds and communities can create in their final cancer for substantial expert and the lung that discusses with granting and razing to company. For some, severe co-agglutination is to pass a designed kingdom and opposition of the lung of their positions. For allegiances, easily younger cemeteries, the strategy to complete draft understanding, all published to the small tumour, exists an meta-analysis of information. In freeing the download modelling biomedical signals of any analgesia, it has commercial at the pt and as Native of the claim to make what are the available goods and voters that parameters are to differentiate and how should the miles Imply studied to use their development. In starring Definitions ...
Looking for passive agglutination? Find out information about passive agglutination. in biochemistry: see immunity immunity, ability of an organism to resist disease by identifying and destroying foreign substances or organisms. Explanation of passive agglutination
The invention relates to agglutination assays and related kits, reagents and devices. In particular methods of assaying small analytes having few epitopes are disclosed, by means of using hub moieties to which multiple analytes may be bound by a first epitope, together with a further moiety capable of binding a second analyte epitope and which is also capable of binding to a detectable particle. Stable agglutinated complexes may be so formed, which may used as the basis for various assay formats.
海词词典,最权威的学习词典,专业出版acidic agglutination是什么意思,acidic agglutination的用法,acidic agglutination翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
The experimental results which have been described demonstrate the following facts:. 1. In the type-specific agglutination reaction, when the organisms are not present in sufficient numbers to absorb completely all the antibodies from the serum, more antibody is bound by cellular S than is required for the process of agglutination.. 2. The excess of antibody thus bound can then unite with additional amounts of the specific substance when this is added in soluble form to the agglutinated material.. 3. If an excess of the free S is added to an agglutinated mass of antibody and bacteria, the organisms are redispersed and in the suspended state are again specifically agglutinable.. 4. When a solution of the specific polysaccharide is added in excess to an homologous immune serum, a prozone is created in which precipitation is inhibited; moreover, if, at this point, type-specific pneumococci are added to the mixture, inhibition of agglutination also occurs.. 5. The reactive substance in the ...
Agglutination: …antibodies usually results in clumping-agglutination-of the red cells; therefore, antigens on the surfaces of these red cells are often referred to as agglutinogens.
The assay off the present Invention is of particular use for detecting drugs, hormones, steroids, antibodies and other molecules circulating in the blood of a mammal or other animal.
Agglutination occurs when blood cells or bacteria clump together, and it is often a response to a wound or injury. Blood cell clumps, called agglutinates, are visible, making them a reliable test for...
[Part 2 of 6 in a series investigating Paulings work on the serological properties of simple substances.] The first four papers published by Linus Pauling and his Caltech colleagues on the serological properties of simple substances described general aspects of the precipitation reactions that occur between antibodies and antigens. This work was spurred by a…
Looking for online definition of agglutination reaction in the Medical Dictionary? agglutination reaction explanation free. What is agglutination reaction? Meaning of agglutination reaction medical term. What does agglutination reaction mean?
Definition of latex agglutination test in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is latex agglutination test? Meaning of latex agglutination test as a legal term. What does latex agglutination test mean in law?
Aim: We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with strain 19.. Materials and Methods: The antibody titers were measured by standard tube agglutination test (STAT), microtiter plate agglutination test (MAT), indirect hemagglutination assay (IHA), and indirect enzyme-linked immunosorbent assay (iELISA) as per standard protocols.. Results: The mean STAT titers were 1.963 0.345 in infected cattle and 1.200 0.155 in healthy vaccinated cattle. The difference was extremely significant (p,0.0001). The mean MAT titers were 2.244 0.727 in infected cattle and 1.200 0.155 in healthy vaccinated cattle. The difference was very significant (p,0.005). The mean IHA titers in infected cattle were 2.284 0.574, and those in healthy vaccinated cattle were 1.200 0.155. The difference was extremely significant (p=0.0002). However, the difference in mean iELISA titers of infected cattle (1.3678 0.014) and healthy ...
Background: Visceral leishmaniasis (VL) is an endemic disease in some parts of Iran. Many techniques have been used for diagnosis of VL, among which the urine based la-tex agglutination test (KAtex) is a promising one. Objective: To compare three diag-nostic tests of VL including KAtex, ELISA and Direct Agglutination Test (DAT) in VL patients and healthy controls in the south west of Iran. Methods: Serum (n = 29) and urine samples (n = 31) were collected from parasitologically confirmed VL patients. Control samples were obtained from healthy individuals (n = 61) and also from patients with infectious diseases other than VL. The collected serum samples were tested by DAT and ELISA using crude antigen from promastigotes of Leishmania infantum and the urine samples were tested by KAtex. Results: Sensitivity and specificity of KAtex for diagnosis of VL was found to be 83.9% and 100%, respectively. Sensitivities of DAT and ELISA were 93.1% and 86.2% and their specificities were 100% and 90.5%, respectively.
Fibrinogen can transform fibrin through an agglutination reaction, finally forming fibrin polymer with grid structure. The density and viscosity of the reaction system changes drastically during the course of agglutination. In this research, we apply an independently-developed piezoelectric agglutination sensor to detect the fibrinogen agglutination reaction in patients with coronary heart diseases. The terminal judgment method of determining plasma agglutination reaction through piezoelectric agglutination sensor was established. In addition, the standard curve between plasma agglutination time and fibrinogen concentration was established to determinate fibrinogen content quantitatively. The results indicate the close correlation between the STAGO paramagnetic particle method and the method of piezoelectric agglutination sensor for the detection of Fibrinogen. The correlation coefficient was 0.91 (γ = 0.91). The determination can be completed within 10 minutes. The fibrinogen concentration in the
The latex agglutination test is a laboratory method to check for certain antibodies or antigens in a variety of body fluids. Learn more about this test here.
Passive Agglutination Tests in which Antigen is adsorbed onto Latex particles which then clump in the presence of antibody specific for the adsorbed Antigen. (From Stedman, 26th ed ...
... ,A Color-Enhanced Slide Agglutination Test for the Rapid Qualitative and Semi-Quantitative Detection of Rheumatoid Factor in Serum.,medicine,medical supply,medical supplies,medical product
Bain, G O., "Agglutinin response to sheep erythrocytes in mice following intra- peritoneal injection of allogeneic spleen cells." (1963). Subject Strain Bibliography 1963. 57 ...
The Importance of Cobalamin (Vitamin B12 in Companion Animals). This year the college will also be hosting Two hour workshops for an added cost of £20 per workshop in the following:. Practical Haematology (cost £20). This workshop covers practical haematology for the veterinary nurse in practice, including interpretation of basic haematology results; performing slide agglutination test; preparing a perfect blood smear and blood cell identification.. Managing the Cardiac Patient (cost £20). In this workshop there will be a practical heart dissection to review the basic anatomy of the cardiovascular system. We will be reviewing common congenital and acquired heart disease along with pharmaceuticals used and nursing care required.. Back to Whats On ...
This study comprises two parts: part one was conducted to isolate E.coli O157: H7 from children in Pediatric Hospital of Karballa city. The bacteria were identified by bacteriological, biochemical and serological methods.( 14 )isolates of E.coli O157:H7 isolated from children with diarrhea (230) and children had urinary tract infection(200) were isolated. Part two of the study was included detection of antibodies against E.coli O157:H7 somatic (inactivated bacterium by heating) and flagellar (inactivated bacterium by formaldehyde) antigens in rabbits by using ELISA test. Twelve adult healthy rabbits were divided randomly for three equal groups A, B and C used for immunization study. All rabbits had negative fecal bacteriological culture of E.coli O157:H7 and serum was collected before immunization. Serum antibodies titers were estimated by using ELISA and tube agglutination test pre and post each immunization. No antibody titers were detected Pre immunization in all rabbits. Elisa test
An immunodiagnostic test card includes a plurality of transparent chambers wherein each chamber includes a quantity of testing material that combines with a patient sample, when mixed, to produce an agglutination reaction. A plurality of indicia are disposed to aid in the manufacture and determining the usability of the cards prior to test and also in objectively grading the agglutination reactions that are formed or lack of agglutination.
Abstract does not appear. First page follows. Introduction All who have used the agglutination test for the detection of fowls that harbor Bacterium pullorum have observed the occurrence of excessive turbidity in many tubes which seriously interfered with accurate reading of the reactions. Hitchner, in 1923, reported that the turbidity resulted from the precipitation of fat that is present in the blood serum of some fowls and that it could be avoided by starving fowls for thirty-six hours before blood samples were drawn. Matthews, in 1926, reported studies which he believed demonstrated that such turbidity was due to the presence of a protein rather than a fatty substance in blood serum of fowls. He stated that this protein substance was soluble in weak alkali solution and that clouding of agglutination tests could be avoided by adding a small amount of sodium hydroxide solution to antigen. Bushnell,
Find all Immune Complexes Agglutination Blood Labs in Ghatkopar West,Mumbai. Now View Prices, Normal Range, Sample Results & Book Online for Immune Complexes Agglutination Blood Labs in Ghatkopar West Mumbai only on | Practo
1. The agglutination inhibition zone, artificially produced by heating, has been studied.. 2. The phenomenon is specific and is dependent upon the presence in the inhibition zone serum of altered agglutinin (agglutinoid).. ...
Definition of group agglutination. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
Students perform a simulated test for the presence of blood on evidence collected from 2 suspects in a murder case. They then use synthetic blood typing to test whether either suspect can be linked to the crime. The simulated agglutination reactions are extremely realistic. Kit contains no blood, blood products, or materials of biological origin, so there is no danger of disease transmission.Refill contains synthetic blood and antisera replacements ...
Students perform a simulated test for the presence of blood on evidence collected from 2 suspects in a murder case. They then use synthetic blood typing to test whether either suspect can be linked to the crime. The simulated agglutination reactions are extremely realistic. Kit contains no blood, blood products, or materials of biological origin, so there is no danger of disease transmission.Refill contains synthetic blood and antisera replacements ...
Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here. ...
The results of this study demonstrate that MAT results are discordant among different laboratories in dogs recently vaccinated against leptospirosis and in dogs with clinical leptospirosis. The percentage agreement among the VDLs was as low as 11% for the vaccinated dogs, and was only 31% for initial titers from the clinical cases, and 27% for the results from the convalescent samples. In addition, the data show that MAT results vary over time in individual dogs with the disease. These observations suggest that MAT titers cannot be relied upon to predict the infecting serogroup in dogs with leptospirosis.. Dogs in this study that were vaccinated with a 4-serovar subunit vaccine developed positive MAT titers at all VDLs. These dogs frequently developed the highest titers to nonvaccinal serogroups. This finding has been reported in a previous study in which dogs vaccinated with a subunit vaccine containing serovars Pomona and Grippotyphosa developed the highest MAT titers to serogroup Autumnalis.8 ...
A patient with a metastatic colon carcinoma was treated by immunoadsorption (IA) therapy using heat-killed, formalin-stabilized protein A-containing Staphylococcus aureus Cowan I as the immunoadsorbent. The patient experienced both subjective and objective positive clinical response without undue morbidity. The patients response correlated well with laboratory findings of decreased concentrations of carcinoembryonic antigen (CEA), immunoglobulin G (IgG), immune complexes (IC) and histopathologic data. The patient underwent surgery following 15 IA treatments; she lived for eighteen months, post-treatment.
A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agglutination test was evaluated with groups of serum samples from patients with leptospirosis and control patients from Hawaii, the Seychelles, Thailand, and The Netherlands. The mean overall sensitivity was 82.3%, and the mean overall specificity was 94.6%. The assay is easy to perform and does not require special skills or equipment. The reagents have a long shelf life, even at tropical temperatures. Together, these factors make the assay suitable for use even at the peripheral level of a health care system as a rapid screening test for leptospirosis. ...
The analysis of 4450 toxoplasma serology results showed that 59 (1.3%) latex agglutination reactions were not confirmed in the dye test. These discrepant results were associated with an unspecified IgM antibody but not associated with kit batch variation, inactivation of sera, concurrent cytomegalovirus infection, or the presence of hepatitis B virus "e" antigen. The latex agglutination test is useful as a screen for toxoplasma infection but false positive reactions do occur. Patients at risk of severe toxoplasmosis should be investigated by additional tests.. ...
I am a 38 years old male, who had severe weakness, dizziness, breathlessness and chest pain for over period of one month last year. It started with a throat infection. Finally, |b|I underwent Widal test, where my paratyphi B was found to be 1:160 and white blood cell (WBC) count was 3500.|/b| Based on this, I was treated for typhoid. But I never had fever in this period. I also underwent ECG and 2D echo cardiogram, which came normal. However, I was diagnosed with GERD. Two months back, I again went for Widal test, where my paratyphi B was found to be 1:20 and white blood cell (WBC) count was 6300. Now, I am again experiencing fatigue and chest pain. Widal test has revealed Typhi O as 1:80 and Typhi H 1:160. WBC came 4300. Is Widal test a confirmation of typhoid? I have no loose motions, fever and do exercise regularly.
OBJECTIVE: To study the predominant Neisseria gonorrhoeae strain types in Pakistan and to evaluate their correlation with fluoroquinolone resistance. METHOD: A total of 314 strains were collected from 2007-2009. Of these 112 strains were randomly selected for serotyping via the coagglutination technique. Fluoroquinolone susceptibility was checked through the E-test method. Chi square was performed to assess the correlation between the strain type and fluoroquinolone resistance pattern. RESULTS: N. gonorrhoeae isolates were typed in two serogroups and 28 serovars. Serogroup WI comprised 40% (n = 45) whereas WII/WIII was 60% (n = 67). Most commonly isolated serovar belonged to serogroup WI namely Aorst (10%). The other predominant circulating serovars of the serogroup WI were Aost (9%) and Ast (8%) and Bsy (8%), Bopyt (5%) and Bprt (4.5%) in the serogroup WII/III. Fluoroquinolone resistance was 98%, with an MIC of 2 microg/mL in 47%, 4 microg/mL in 36% and | 32 microg/mL in 12% of the isolates. On
Toxoplasmosis is the major parasitic disease affecting sheep. It is important for veterinary medicine, animal science and public health since it causes reproductive and economic losses in the herd, as well as damaging human health due to consumption of contaminated meat and milk, which can facilitate zoonotic transmission. Detection of Toxoplasma gondii in ovine milk and lack of data in the literature describing differentiation between acute and chronic disease for this species stimulated the elaboration of the present research project. To achieve the aim of this study, the animals were allocated to two groups of 20 ewes each, of which group 1 was composed of animals with positive serology and group 2 with negative serology. Acute and chronic stages of the disease were differentiated by modified direct agglutination test (MAT), in which antigens were fixed with formalin (MAT-AF) and methanol (MAT-AM). The parasite was detected in milk by polymerase chain reaction (PCR), and the molecular ...
Toxoplasma gondii is a zoonotic protozoan parasite that thrives in Estonia. In this nationwide cross-sectional study, we tested sera from 3991 cattle, collected from 228 farms in 2012-2013, for anti-T. gondii immunoglobulin G antibodies using a commercial direct agglutination test. Titer of 100 was set as cut-off: samples that tested positive at the dilution 1:100 were defined as positive. The app ...
Agglutination occurs when an antibody interacts with antigen, resulting in cross-linking of the antigen particles by the antibody. This eventually leads to clumping. "Agglutination" comes from the Latin root agglutinare. "Agglutinare" means, "to glue". Cross-linking, or cross-matching is done to determine matches. Agglutination may occur when an unideterminate, multivalent antigen interacts with a single antibody. It may also occur if a multi determinate, univalent antigen interacts with at least two distinct antibodies. Cross Agglutination: when an antibody that is raised against a similar antigen agglutinates that antigen. Group Agglutination: when a collection of similar organisms is agglutinated by an agglutinin that is specific for that collection. Applications for the agglutination test BLOOD TYPING There are different blood groups like A,B, AB and O. The different blood types are differentiated according to the types of proteins on the surfaces of the red blood cells. Human red blood ...
The gonococcal isolates from 15 contact pairs and three large contact groups were examined using various methods to assess the stability of different typing markers. With the exception of one contact group which showed variable proline requirements, the auxotypes were stable during natural transmission. Serogrouping using the coagglutination method to detect W and M antigens was undertaken. The lipopolysaccharide M antigens were readily lost and gained during transmission whereas the protein W antigens represented stable markers and are thus useful for epidemiological studies.. ...
Why dont antibodies of o blood group does not cause agglutination reaction with any of antigens in other blood group? Study Blood Types Review.
serum agglutinin An antibody which coats erythrocytes; the cells do not agglutinate when suspended in saline, but do agglutinate when suspended in serum or other protein media such as albumin. ...
0084] In a preferred embodiment the presence of Gallibacterium is determined by an immune test. An immune test uses monoclonal antibodies or polyclonal antisera specific to Gallibacterium. The generation of monoclonal antibodies is known in the art (Kearney, J. F., Radbruch A., Liesegang B., Rajewski K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits construction of antibody-secreting hybrid cell lines. J. Immunol. 1979, 123: 1548-1550., Kohler, G., Milstein, C. Continous culture of fused cells secreting antibody of predefined specifity. Nature 1975, 265: 495-497) Immune tests include the methods of detection known in the art such as the ELISA test (enzyme-linked immuno-sorbent assay) or the so-called sandwich-ELISA test, dot blots, immunoblots, radioimmuno tests (radioimmunoassay RIA), diffusion-based Ouchterlony test or rocket immunofluorescent assays) or agglutination tests (rapid plate or micro-plate agglutination tests). Another immune test is the so-called ...
Question - Typhoid infection, fever, abdominal pain. Widal test shows S typhi O, H positive. Recurring infection?. Ask a Doctor about diagnosis, treatment and medication for Typhoid, Ask an Internal Medicine Specialist
books.google.comhttps://books.google.com/books/about/Essentials_of_practical_microtechnique.html?id=HWLXAAAAMAAJ&utm_source=gb-gplus-shareEssentials of practical microtechnique ...
... : Latex agglutination tests for the detection of C-reactive protein. Results provided in