AIMS--To determine the best medium for the primary isolation of Helicobacter pylori. METHODS--Sixty six gastric mucosal biopsy specimens frozen in 1 ml Cysteine Albimi media with 20% glycerol from 22 histologically proven H pylori infected patients were cultured on brain heart infusion agar (BHIA) with 7% fresh whole defibrinated horse blood, egg yolk agar (EYA), Columbia blood agar-cyclodextrin agar (CBA-Cd), and commercial trypticase soy agar (TSA) supplemented with 5% sheep blood. RESULTS--Successful primary isolation of H pylori was 96% with BHIA, 78% with TSA, 64% for EYA, and 32% with CBA-Cd. Colonies appeared earlier on BHIA (4.7 +/- 0.1 days, 5.3 +/- 0.4 days, 5.3 +/- 0.4 days, and 7.1 +/- 0.9 days for BHIA, TSA, EYA, and CBA-Cd) and there were more colonies on BHIA than on CBA-Cd, EYA or TSA (599 +/- 88, 104 +/- 66, 260 +/- 107, and 358 +/- 89, respectively). CONCLUSIONS--Success of a medium for passage of isolates apparently does not reliably predict usefulness for primary isolation. ...
Chocolate agar (CHOC) or chocolate blood agar (CBA) - is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80 °C. Chocolate agar is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae and Neisseria meningitidis. In addition, some of these bacteria, most notably H. influenzae, need growth factors such as NAD (factor V) and hemin (factor X), which are inside red blood cells; thus, a prerequisite to growth for these bacteria is lysis of the red blood cells. The heat also inactivates enzymes which could otherwise degrade NAD. The agar is named for the color and contains no actual chocolate. Chocolate agar with the addition of bacitracin becomes selective, it is most critically, for the genus Haemophilus. Another variant of chocolate agar called Thayer-Martin agar contains an assortment of antibiotics which select for Neisseria ...
Mug Agar found in: Nutrient Agar w / MUG, 14mL, ECD MUG Agar, VRB MUG Agar, MUG Nutrient Agar, Plate Count MUG Agar, ECD MUG Agar, MacConkey MUG Agar, E...
Depending on the agar concentration, the film of liquid on the surface of the agar may be thick enough (?) to allow bacteria that have flagella to swim along the surface. Because the bacteria often move side-by-side in large groups this behaviour is called swarming. Often swarming on agar surfaces is facilitated by surfactants that the bacteria produce, which reduce the surface tension of the aqueous layer. Ive always assumed that bacteria living in soil and on other surfaces produce such surfactants as a way of getting surface-adsorbed nutrients into solution (thats how the surfactants we use in soaps and detergents do their job), but maybe surfactants are also advantageous for moving across surfaces with air-water interfaces, such as damp soil. The side-by-side cell orientation and movement may also be a consequence of surface-tension effects, as illustrated in this sketch ...
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The overall performance of SCITB agar plus the spot indole test for the detection of K. oxytoca was superior to that of MacConkey agar by McNemars test (P = 0.022). The sensitivities and specificities of the use of SCITB agar plus the spot indole test and MacConkey agar alone as a screening test were analyzed and are listed in Table 1 ...
BioAssay record AID 771708 submitted by ChEMBL: Antibacterial activity against Escherichia coli ATCC 25922 at 25 ug/disk after 24 hrs by agar diffusion assay.
Figure 3: Colonies from Sheeps blood agar plate. MICROBIOLOGY DIAGNOSIS:. Streptococcal pneumoniae is a Gram positive cocci arranged in chains and pairs. On blood agar plates, colonies are surrounded by a green alpha-hemolytic zone (due to production of pneumolysin which breaks hemoglobin into a green pigment). Optichin disks, which contain a quinine derivative, selectively inhibit the growth of S. pneumoniae on blood agar plates, and are used to differentiate pneumococcus from other viridans streptococci [1]. Susceptibility testing of the isolate revealed that it was sensitive to levofloxacin, erythromycin, tetracycline, sulfamethoxazol, chloramphenicol, and vancomycin. The MIC for penicillin and ceftriaxone were 0.023 µg/mL and 0.016 µg/mL, respectively.. This patients recurrent Streptococcus pneumoniae bacteremia, as well as his hyper-IgG, hypo-IgA and IgM prompted a search for an underlying immune disorder. PATIENT MANAGEMENT:. Upon identification of the Streptococcal pneumoniae, the ...
How is cycloserine-mannitol-blood agar , and cycloserine-cefoxitin agar abbreviated? M-CCA stands for cycloserine-mannitol-blood agar , and cycloserine-cefoxitin agar. M-CCA is defined as cycloserine-mannitol-blood agar , and cycloserine-cefoxitin agar rarely.
Specimen Collection: Consult listed references for information on specimen collection.(1,2,4,5,7-10) Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport media and refrigerated until inoculation. Consult the listed references for information regarding the processing and inoculation of specimens.(1,2,4,5,7-10) Method of Use: Allow media to warm to room temperature, and the agar surface to dry before inoculating. Inoculate and streak the specimen as soon as possible after collection. If the specimen to be cultured is on a swab, roll the swab over a small area of the agar surface. Streak for isolation with a sterile loop. Incubate plates in an inverted position. The caps of tubed media should be slightly loosened. Once inoculated, media should be protected from light and incubated aerobically at 25-35ºC. with increased ...
Specimen Collection: Consult listed references for information on specimen collection and processing of food, dairy, water samples, and other materials of sanitary significance. (1-8) Prior to inoculation, warm prepared media to room temperature. For melting bottled media: Autoclave at 121ºC. for one to three minutes or until melted. Alternatively, a covered, boiling waterbath (100ºC.) can be used. There should be enough water in the waterbath to reach the media line. A covered waterbath will help to reach and maintain the temperature. Heat in waterbath until melted. Spread Plate Method: 1. Prepare decimal dilutions in sterile diluent to obtain 30-300 CFU per plate. 2. Aseptically inoculate agar surface with 0.1ml of well mixed diluted sample. 3. Using a sterile spreader device, spread the dilution evenly over the surface of the agar. 4. Incubate plates aerobically for 48 +/- 2 hours at 35ºC. Pour Plate Method: 1. Melt agar by placing in a boiling waterbath until liquified. 2. Cool media to ...
Yeast And Mold Agar found in: M-Green Yeast and Mold Bottled Agar, Yeast Mold Agar (YM), Bottle Yeast Mold Agar 500G, Petrifilm™ Rapid Yeast and Mold Count..
Agar plates are commonly used in laboratories to grow bacteria for research purposes. The agar on the plate serves only as a medium to hold the bacteria, while added nutrients help the bacteria grow. Laboratories may use ready-made plates because they are consistent and faster than preparing plates from scratch.
Agar plate preparation. Young scientist pouring liquid agar growth medium into Petri dishes in a laminar flow cabinet. The agar will set into a gel when cool and is used as a medium for culturing micro-organisms such as bacteria and fungi. - Stock Image C028/7989
i have given an assignment to identify the fungi strain from about 50 of samples culture on agar plate. my lecturer have me to find the antibiotic and antifungal agent to add into the agar medium and redo the screening process of these samples ...
Hmmh... as far as I can tell that looks quite normal non-hemolytic growth typical to Bacillus species, though the angle and light are not ideal for spotting it. I am not sure what causes the darker coloration of the agar (Id call it deep red rather than black from what I see), but it is a common phenomenon possibly related to the use of other nutrients in the medium and to the change of pH or something. Bacteria break down and secrete various compounds, so I would not be surprised if some of those cause the darkening of the agar. Admittedly, I do not know the exact answer so if someone does, let us know. And the dark spot seems to be created by the administration of bacteriophages, as indicated in the text ...
If long term obsrvation is desired, a special slide needs to be made. Place masking tape on a slide, with a hold cut in the middle. Put a small drop of liquified NG agar on the slide and place a siliconized coverslip immediately on the agar. When the agar solidifies, remove the coverslip. Just prior to use, place a very small amount of E. coli on the agar, transfer a worm to the agar, and place a coverslip over the agar and the tape. Once the worm takes to eating the bacteria, it will settle down and can be readily observed for hours. ...
A blood agar plate is put in boiling water for about 1 hour until its colour changes from red to chocolate brown. Heating converts Haemoglobin into hematin ...
my competent cell grow on any agar plate - posted in Microbiology: I have been given a batch of XL1 blue cells. I made them competent using calcium chloride procedure. To test them, i plated them out on agar plate containing: Kanamycin, Tetracyclin, Ampicilin and control. After overnight incubation, there is bacteria colonies in all plates. These colonies are so dense we cant not see them separately.Since I have kept my original stock of cells before making them competent, I did the same t...
Introduction: Materials and Methods: 1.Using the swirled up Hay infusion from Lab 1 and 2, 100 ml of the hay infusion culture was added to 10mls of nutrient broth into a tube. This made a 1:100 dilution or 10^-2. Next 100microliters from that tube was placed into 10mls of nutrient broth to make a dilution of 1:10,000 or 10^-4. Next 100microliters from tube 4 was placed into 10mls of nutrient broth to make a dilution of 1:1,000,000 or 10^-6. Next 100microliters from tube 6 was placed into 10mls of nutrient broth to make a dilution of 1:10,000,000 or 10^-8. 2. Four nutrient agar plates were labeled. 10^-3, 10^-5,10^-7,10^-9. Another set of Agar plates with tetracycline were labeled with the same labels. 3. 100 microliters of the 10^-2 solution was placed on the surface of the 10^-3 agar plate and the 10^-3 agar plate with tetracycline. 100 microliters of the 10^-4 solution was placed on the surface of the 10^-5 agar plate and the 10^-5 agar plate with tetracycline. 100 microliters of the 10^-6 ...
Sigma-Aldrich offers Sigma-M7408, MacConkey Agar for your research needs. Find product specific information including CAS, MSDS, protocols and references.
Microbiological quality control of pharmaceutical water systems is of importance in ensuring that trends in contamination are detected and responded to. This is not least because water is a niche environment for many types of microorganisms and a vector for their transfer. Trending relates to actual microbial counts recorded, incidents and the types of species recovered. To facilitate species identification, microorganisms need to be subcultured from the isolation medium (R2A agar in Europe). Transfer onto the wrong media can result in the microorganism not growing. This paper describes research into three different media for subculturing: low nutrient (R2A); highly nutritious (TSA) and medium nutrient (R3A) and concludes that a higher recovery is obtained where R3A agar is used ...
We got back the agar that we made yesterday and we are supposed to interpret results. The different agars contain different substances which only allows some type of bacteria to grow on such as the MacConkey Agar (MAC) only allows gram-negative bacteria to grow. As for the agar that are in tubes, there was no bacteria on all of the samples except 1. I feel that it was because we did not smear it properly and hence we did not see any bacteria growth. We also get to use the microscope to see the different specimens. ...
The best way to grow bacteria on agar depends on the type of bacteria you want to grow. Agar is a substance extracted from red algae that forms a gel when mixed with water. It is a good medium for growing bacteria because it can be poured into forms such as a plate or test tube; it provides nutrients and moisture; and ...
PERFORMANCE OF THE ASSAY (Direct plate assay): Top agar in top agar tubes was melted by heating to 45 ± 2°C. The following solutions were successively added to 3 ml molten top agar: 0.1 mL of a fresh bacterial culture (10^9 cells/mL) of one of the tester strains, 0.1 mL of a dilution of the test item in DMSO and either 0.5 mL S9-mix (in case of activation assays) or 0.5 mL 0.1 M phosphate buffer (in case of non-activation assays). The ingredients were mixed on a Vortex and the content of the top agar tube was poured onto a selective agar plate. After solidification of the top agar, the plates were inverted and incubated in the dark at 37.0 ± 1.0 °C ...
agar polymerization - posted in General Lab Techniques: Hello everybody! I was wondering what could stop agar from polymerizing? I poured plates (5g agar, 0,9 g NaCL in 100ml H20) and left them to polymerize but in 24 hours they are still half liquid! What did I do wrong?
After meeting in art school on the outskirts of Paris, Clara and Armand came together to form techno pop duo Agar Agar. Hard synth bass lines bump behind Claras dark and sensual voice, at times giving their 80s era sound an almost angry intensity. Their first album Cardan was released in late 2016 and the pair is currently on tour in France.. ...
BioAssay record AID 1084736 submitted by ChEMBL: Antimicrobial activity against Escherichia coli 5933 after 24 hr by agar cup diffusion technique.
Ive got petri dishes, Ive got agar powder and dry malt extract. I did a prior run with ground up agar sticks into MEA, but when I put it into the petri dishes I had no idea  how much 
Nutrient agar medium for general bacteriological work. Supplied as 100cm3 lots in screw-cap bottles. This bulk pack is cost-effective, but the mediumtakes longer to melt so there is some risk of caramelisation, or contamination if not all used immediately. Pack size 2.
The noble agar assay is based on the principle that the cancer cells are anchorage-independent and do not show contact inhibition. This type of soft agar assay can give insight into two important properties of a subset of cancer cells. Firstly, the tumorigenic efficiency of cancer cells can be determined based on number of colonies that emerge. This is based on the ability of cells to grow unattached and to remain suspended in agar. Secondly, the self-renewal properties of cancer cells can be assessed based on the types of progeny produced in noble agar.. ...
Triple Sugar Iron Agar and Its Use and Interpretation see Difco Manual, 9th Ed, (1953) pp. 166-168 (Table on p. 161.) INTRODUCTION: Triple Sugar Iron medium is a differential medium that can distinguish between a number of Gram-negative enteric bacteria based on their physiological ability (or lack thereof) to: a. metabolize lactose and/or sucrose…
Place a coffee filter in a strainer and hang the strainer over the opening of the clear bowl.. Slowly pour the broth into the filter, making sure that the broth doesnt overflow from the sides of the filter. If the filter becomes clogged with particles, remove the used filter, replace with a clean filter and continue pouring the broth.. Remove the strainer and filter from the top of the bowl and observe whether the broth appears clear (i.e., there are no suspended particles in the liquid). If particles are floating in the liquid, repeat Steps 1 and 2 using a second clear bowl and a fresh coffee filter.. Place the pot on the stove and pour the clear broth into the pot.. Add 16 ounces of distilled water to the pot containing the broth.. Turn on the stove and adjust the heat to medium-low. Heat the broth to a simmer (i.e., small bubbles appear near the side of the pot).. Add 1 teaspoon of the agar-agar powder to the simmering broth (approximately 2.5 grams) and stir the mixture until the powder is ...
At 11:17 AM 5/17/01 -0800, Karen Larison wrote: ,We first embed them in a ,melted agar-sucrose matrix, which allows us to orient them very ,precisely within the block for serial sectioning. Karen, What is the formula for the agar and sucrose? I am interested in trying this. Do you need to let the tissues infiltrate in the agar for a while? Connie McManus We then let the ,block solidify and then sink it in in sucrose before sectioning. The ,nice thing about these agar blocks is that they ribbon nicely. You ,can cut a series of sections with the antiroll plate, then use a ,brush to straighten them out, and then pick them up. Using this ,method, we are better able to position these tiny sections on a ,slide, we are less likely to experience leaping sections, and we ,can get 70-100 sections on one slide in very short order! , ,We also use Tissue Freezing Medium (made by TBS, available from ,Fisher Scientific) to adhere our agar block to the chuck, and find ,that this curls less, and appears to ...
PRE-INOCULATION: The Petri dish and agar are sterilised to kill any unwanted bacteria. This is achieved by passing the inoculation loop through a flame so that it becomes sterile. INOCULATION: The now sterile loop is dipped in a suspension of bacteria which are transferred to the agar (made of carbohydrates). The lid of the Petri dish is replaced quickly to prevent microbes from the air entering. POST-INOCULATION: The Petri dish is sealed with tape to prevent microbes from the air entering and contaminating the culture. It is important to not seal the dish all the way to ensure oxygen can enter. Otherwise harmful anaerobic bacteria would grow. The Petri dish is then incubated at 25oC max to allow the bacteria to grow. Above 25oC pathogens might grow instead. ...
1.2 Use half-strength (nutrient and agar) tryptic soy agar as the inoculum vehicle for bacteria and half-strength potato dextrose agar as the inoculum vehicle for mold conidia. Use of half-strength agars may reduce undue neutralization of an antimicrobial due to excessive organic load. ...
Each year, many people die of nosocominal infections - infections that the patients acquired in a hospital. However, these infections would be preventable in large part through better hygiene programs. Diseases caused by multi-resistant pathogens are especially critical because therapy options are very limited in these cases. But actually, what are pathogens exactly and where do they come from? In order to answer these questions, we start the teaching module with the topic "Microorganisms in our Environment". In this part, we explain which germs we encounter every day, and how be can best protect ourselves from them. In order to demonstrate how many and which microorganisms are our steady companions, we conduct an experiment with the students. A pair of students received 3 agar plates: one with regular LB agar, one plate with agar supplemented by an antibiotic, and one selective agar plate for yeasts and fungi. Equipped with the plates, the students wander through their school and take contact ...
Does anyone have any experience, tips, or suggestions for a multispore agar innoculation? I have spore prints, and I have read some of the documents regarding techniques. Any advice though would
i had kept the rest of the almond milk in the fridge this whole time and adding the cold milk to the thickened heated agar milk mixture instantly gelled it! so it got clumpy! this is what i was trying hard to avoid in the first place, ugh! i tried beating it at high for a longer time but it still was clumpy. (the clumps taste more like clumps of agar than clumps of extra thick chocolatey stuff, so kinda not so great) oh well ...
An apparatus for automatically transferring a desired one of bacterial colonies grown in a medium in a culture Petri dish onto a medium in a test Petri dish or a test tube by using a bacterial colony pick-up/transfer element of the disposable type or a bacterial colony pick-up/transfer element of the reuse type and by using various mechanisms controlled by a computer.
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View the plots, npcs, items, lifeforms, locations, articles, dungeons, and societies written by Agars at the role playing site of Strolens Citadel. The Role Playing Game Workshop: Read. Post. Play.
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Confocal and apotome microscopy images and a schematic representation of an A. fumigatus biofilm (StA) (Beauvais et al., 2007). (A1 to A4) Depth projections from a confocal image stack recorded from an StA culture after calcofluor white staining (24 h of growth; Plan-Apochromat, 20X/0.75 objective). Panels A1 to A3 are sections of a 24-h StA culture from the agar surface (in red) to the air surface (blue). A4 is a reconstitution of panels A1 to A3. (B1 and B2) 3D images of an StA culture (Plan-Neofluar; 40X objective, 0.8 numerical aperture), showing the vertical growth of the fungus (16 h of growth [B1]), followed by the appearance of extracellular material holding the hyphae together (20 h of growth [B2]). (C) Schematic representation of growth of an A. fumigatus biofilm compared to colony formation ...
summit ,- function(x,y,output){ y,- Before chrom ,- x[1] start ,-x[2] end ,-x[3] startLine ,- y[which((y$V1 == chrom) & (y$V2==start)),] endLine ,- y[which((y$V1 == chrom) & (y$V3==end)),] Subset ,- y[which((y$V2 ,= startLine$V2) & (y$V3 ,= endLine$V2))] maximum ,- Subset[which(Subset$V4 == max(Subset$V4))] output ,- print(maximum) } apply(Peaks,1,summit,output = peaks_list.bed ...
Agar bacteriological powder with minimal amounts of salt or extraneous matter, for use as a solidification agent in the preparation of microbiological culture media.
Coba kau tunjuk satu bintang. Coba kau tunjuk satu arah perjalanan agar bensin di mobil kita tidak habis untuk berputar-putar tanpa tujuan. Coba kau tunjuk satu tujuan agar tenaga dan pikiran kita tercurah untuk mencapainya. Coba kau tunjuk sebuah sasaran yang jelas agar kita mudah mengarahkan kemampuan kita untuk menggapainya. Coba kau tunjuk sebuah pilihan yang jelas agar gaji-mu tidak tersebar kemana-mana tanpa satu pun hal-hal berarti yang kamu miliki ...
Lift the top four plates plus the lid of the bottom-most plate so that only the bottom part of the bottom-most plate is on the bench. Carefully tilt the agar flask and pour the agar into the empty plate just until the surface is covered with agar. Do not over-pour ...
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Pada kesempatan ini, Binmas mengajak warga agar bersama sama di wilayah untuk mengantisipasi curat, curas dan curanmor dan congkel spion, serta selalu mewaspadai dan mencegah terjadinya curanmor curas dan curat dilingkungan kawasan pemukiman warga optimalkan pam swakarsa.. Kemudian Binmas mengimbau agar memantau dan mengawasi segala bentuk guantibmas terhadap lingkungan di sekitarnya. Apabila terjadi guantibmas agar segera menghubungi pihak Kepolisian terdekat atau polsubsektor terdekat dan tidak main hakim sendiri apabila ada pelaku tindak pidana tertangkap tangan. Tujuan patroli dialogis ini untuk mencegah bertemunya niat dan kesempatan di lokasi-lokasi rawan guantibmas serta menggali informasi yang ada di masyarakat sebagai masukan dalam melaksanakan tugas polri untuk terciptanya wilayah yang kondusif.. (Tribratanews.com). ...
Testul de identificare specie: Examen microscopic nativ si colorat, cultură și identificare bacteriana. Proba de sensibilitate la antibiotice prin urmatoarele metode: Microdiluţie, Agar difuzie Kirby-Bauer, Agar difuzie E-test Probe din: Puroi. ...
Testul de identificare specie: Examen microscopic nativ si colorat, cultură și identificare bacteriana. Proba de sensibilitate la antibiotice prin urmatoarele metode: Microdiluţie, Agar difuzie Kirby-Bauer, Agar difuzie E-test Probe din: Materii fecale. ...
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Extracto de ciertas especies de algas rojas que se usa como gelificante en microbiología y en la preparación de alimentos. Se emplea mucho en los laboratorios como medio inespecífico para cultivar bacterias, hongos y algunas algas.. ...
Salah satu masalah yang selalu dihadapi oleh orang perempuan ialah haid tidak teratur. Haid tak lancar boleh beri banyak kesan kepada kehidupan seorang wanita
Salah satu masalah yang selalu dihadapi oleh orang perempuan ialah haid tidak teratur. Haid tak lancar boleh beri banyak kesan kepada kehidupan seorang wanita
Hal tersebut merupakan langkah Kepolisian dalam menciptakan situasi kamtibmas dilingkungan masyarakat agar tidak berjiwa apatis dalam menyikapi setiap kejadian terutama yang menyangkut keamanan lingkungan.. "Kami himbau masyarakat agar berperan aktif dalam menjaga keamanan lingkungan dengan menanamkan sikap peduli terhadapa setiap kejadian," ungkap Kanit Binmas Aiptu Didi Abadi.. [Tribratanews.com/Humas Polsek Cidahu] ...
Salah satu bagian tubuh kita yang paling terlihat dominan adalah bahu kita. Jika bahu terlatih dengan benar, maka tubuh akan terlihat lebar dan kuat. Namun bahu
Das Pump Set mit Hantelstange, Grifffläche, Aufnahmen Chrom, ca. 140 cm und ca. 2 kg, 2 Federverschlüssen für ein perfektes Training
Kliniixslim since 2005.. Halo sahabat instagram semua nya, saya dr.Imelda Tjoe dipl.AAAM, kliniixslim sudah berdiri 13 thn dikota medan, tetapi sejak saat tahun 2009 sampai sekarang, saya sudah mengaplikasikan / menyuntikan FILLER, BOTOX , TANAM BENANG, untuk merubah bentuk wajah saya yg tadinya bulat, lebar, petak serta bibir yg asimetris. Semua ini saya kerjakan sendiri di wajah saya ( menyuntikan diri sendiri ) , utk menjaga anti aging pada wajahku agar tetap segar dan kelihatan muda ...
Jadwal Maintenance Rutin di Jayatogel Jadwal Maintenance Rutin di Jayatogel - Maintenance yang biasanya disebut perawatan / pemeliharaan, adalah aktivitas untuk merawat sebuah sistem agar dapat bekerja dengan baik dan benar ...
Jadwal Maintenance Rutin di Jayatogel Jadwal Maintenance Rutin di Jayatogel - Maintenance yang biasanya disebut perawatan / pemeliharaan, adalah aktivitas untuk merawat sebuah sistem agar dapat bekerja dengan baik dan benar ...
Program ini untuk Wanita Diberkati agar keluarganya hidup maksimal menikmati janji-janji Allah. Diutamakan bagi yang akan/sudah menikah Angkatan XI: 21-22 Juli 2012 MDC Mega Development Center Gadok Jl. Raya Pasir Muncang Km. 3,5 No. 316 Desa Sukamaju, Kecamatan Megamendung, Bogor Registrasi/Daftar Ulang pk. 07.30 WIB Investasi Rp 495.000 Pembicara: Heidi Awuy Lisna Arifin Emny June…
KELELAHAN OTOT Pengertian Kelelahan adalah suatu mekanisme perlindungan tubuh agar tubuh terhindar dari kerusakan lebih lanjut sehingga terjadi pemulihan setelah istirahat. Penyebab terjadinya kelelahan otot A.Teori Kimia menjelaskan bahwa terjadinya kelelahan otot karena: Sirkulasi darah terhambat karena terjadi vasokontriksi. Menurunnya kadar gula di dalam darah yang akan mempengaruhi produktifitas kerja otot. Meningkatnya sisa metabolisme berupa…
Menurut Legenda, Penyair kuno, Aeschylus, terbunuh gara-gara kura-kura. Aeschylus berkepala botak sehingga seekor burung rajawali salah mengira kepalanya adalah batu. Rajawali tersebut menerbangkan kura-kura dan dijatuhkan ke batu agar tempurungnya pecah. Sang penyair pun langsung tewas seketika. [Dramatis ya ...
The new vaccine is also adapted to the type of rotavirus most commonly found in sub-Saharan countries. It is affordable, with a price of under US$2.50, which is much cheaper than the lowest price of rotavirus vaccines currently available. This should allow it to be rolled out quickly as part of routine immunization programmes. The new vaccine, manufactured by Serum Institute of India Pvt Ltd, will also fill the current supply gaps for the existing vaccines ...
Looking for online definition of Sabouraud dextrose agar in the Medical Dictionary? Sabouraud dextrose agar explanation free. What is Sabouraud dextrose agar? Meaning of Sabouraud dextrose agar medical term. What does Sabouraud dextrose agar mean?
There is growing concern regarding the contribution of infilled turf fields on increased athlete infections. Abrasions that occur on these fields create a port of entry for pathogens such as Staphylococcus aureus that are present on the athletes skin or possibly on the fields surface. This study compares the occurrence of microbial populations on two infilled synthetic turf fields (year old turf vs. 6 year old turf) in three locations. Both fields were sampled once a week for at least 14 weeks (exact number varied on field and location) during the late summer and fall of a football season. Sites sampled included the sidelines, the middle of each field, and the end of each field. Tryptic Soy Agar was used to determine total microbial load, Mannitol Salt Agar for Staphylococcus, and Eosin Methylene Blue Agar to count the number of enteric organisms such as Escherichia coli. Much higher microbial populations were found on the older turf field with as much as a 104 increase over similar locations ...
Acumedia BD / Difco BD / BBL Oxoid Merck Violet Red Bile Agar (7165) 211695 N/A CM0107 1.01406 Intended Use Violet Red Bile Agar is used for the enumeration of coliforms in food and
A 38-year-old woman presented to her clinician with a red, swollen right finger. The patient reported a dog bite two days prior. The clinician submitted a swab of the finger for Gram stain, aerobic, and anaerobic bacterial cultures. The Gram stain is shown in Image 1. After 24 hours of incubation, bacterial grown was noted on sheep blood agar (Image 2) but not the eosin methylene blue agar. A drop oxidase was positive as shown in Image 2. ...
Objectives: Removable surface-protecting disposable plastic barriers and disinfectant solutions are commonly used in dental clinics in order to sterilize surfaces and instruments from contaminations. In this study we isolated and identified 3 distinct bacteria from plastic barriers at the dental clinic, and compared the bactericidal effect of 5 different commercially available disinfectants on these bacteria. Methods: After dental procedures, bacteria were collected from surface-protecting plastic barriers at the dental clinic. Bacteria were cultured aerobically on Trypticase Soy Agar plates at 370C for 24hr, and 16S RNA analysis was used for the identification of 3 isolated and selected red, yellow, and white bacterial colonies. Bacteria were streaked on agar plates, exposed to the aerosol spray of Cavicide, Micrylium, OPTIM 33TB, UNISEPTA Plus and PureGreen-24 disinfectant solutions. Plates were incubated for 24hr to determine the extent of bacterial growth on the agar surface. Results: ...
Kingella oralis is a gram-negative, bacilli bacteria that was first observed by Chen et al in 1989, who were investigating the presence of Eikenella corrodens in the human oral cavity.[2] This isolate was further investigated by Dewhirst et al in 1993, who identified several characteristics that distinguished the two species from each other including colony morphology on agar and acid production from sugars. In early identification and research into K. oralis, the bacteria was successfully grown on Trypticase soy agar plates that contain 5% sheep blood, 5mg of hemin per litre and 0.5mg of menadione per litre at 37°C in a humidified anaerobic chamber containing 5% CO2.[3] However the specific growth requirements of K. oralis are still unknown. K. oralis is found in both supragingival and subgingival plaque, as well as in mucosa and saliva.[4] There is a high association between K. oralis presence and periodontitis.[3][4] Therefore research into K. oralis can potentially aid in periodontitis ...
Blood Agar and Hemolysis Certain bacterial species produce extracellular enzymes that lyse red blood cells in the blood agar (hemolysis). These hemolysin (extotoxin) radially diffuses outwards from the colony (or colonies) causing complete or partial destruction of the red cells (RBC) in the medium and complete denaturation of hemoglobin within the cells to colorless products.. Four types of hemolysis are produced in Sheep blood agar by Streptococci namely; Alpha hemolysis, Beta hemolysis, gamma hemolysis and alpha prime or wide zone alpha hemolysis.. Hemolysis is best observed by examining colonies grown under anaerobic conditions or inspecting sub-surface colonies.. How does one know if the colonies they are observing on a plate have caused alpha hemolysis or beta hemolysis?. Note: To know the type of blood agar hemolysis, the blood agar plate must be held up to a light source and observed with the light coming from behind (transmitted light).. If either type of hemolysis is present, then one ...
Blood Agar and Hemolysis Certain bacterial species produce extracellular enzymes that lyse red blood cells in the blood agar (hemolysis). These hemolysin (extotoxin) radially diffuses outwards from the colony (or colonies) causing complete or partial destruction of the red cells (RBC) in the medium and complete denaturation of hemoglobin within the cells to colorless products.. Four types of hemolysis are produced in Sheep blood agar by Streptococci namely; Alpha hemolysis, Beta hemolysis, gamma hemolysis and alpha prime or wide zone alpha hemolysis.. Hemolysis is best observed by examining colonies grown under anaerobic conditions or inspecting sub-surface colonies.. How does one know if the colonies they are observing on a plate have caused alpha hemolysis or beta hemolysis?. Note: To know the type of blood agar hemolysis, the blood agar plate must be held up to a light source and observed with the light coming from behind (transmitted light).. If either type of hemolysis is present, then one ...
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SOP: Cloning of PCR Product. Add 1 ml room-temperature LB broth (without ampicillin) to the tube. A-9518) agar plate pre-spread with 20 µl X-Gal (50 mg/ml.haemophilus ducreyi. FAQ. Haemophilus ducreyi; Haemophilus;. and then to plate it on blood agar and chocolate agar.thoroughly and pour plates. YEPD (YPD) PLATES: Agar 20 g. Peptone 10 g. Yeast Extract 900 ml distilled water: 5 ml of 1 M HCl (do not mouth pipette) 20 g. Agar Autoclave.1 Genomic tools and cDNA derived markers for butterflies. the library was plated out on LB agar plates with Xgal blue/white 116 screening and 0.1% ampicillin,.. ...
thoroughly and pour plates. YEPD (YPD) PLATES: Agar 20 g. Peptone 10 g. Yeast Extract 900 ml distilled water: 5 ml of 1 M HCl (do not mouth pipette) 20 g. Agar Autoclave.Préparation de membranes pour. Ampicilline A~P 100 Carbeniciline Cb 40 Kanam ycine Km 20. - Milieu LB agar:.Lb agar with ampicillin, ampicillin agar plates - wwgcsa. What is the best ampicillin to chloramphenicol ratio for.. TP biorad (B)TP sauce Paul Éluard (PE) Jour 1 Préparation de 8 milieux LB (B)Couler. Ampicilline et Arabinose. de soja 5 g Chlorure de sodium 5 g Agar.Periplasmic Expression of a Novel Human Bone Morphogenetic Protein-7 Mutant in. of a Novel Human Bone Morphogenetic Protein-7 Mutant in. in LB agar and LB Broth.Préparation de la recette: Râpez la plaquette de chocolat blanc en fins copeaux ou hachez-la à laide dun couteaux-scie. Rassemblez les copeaux dans un saladier.Inoculum and plasmid preparation. [LB] medium) overnight,. (LB medium containing, per liter, ampicillin, 100 mg;.Préparation du milieu ...
HI I HOPE YOU CAN HELP ME. I PERFORMED A TEST WHICH IS A UNKNOWN THEY GAVE US 2 BACTERIA ONE IS S.EPIDERMIS AND S. AUREUS. THE FIRST TEST WAS THE COAGUALSE TEST WHICH I GOT POSSITIVE FOR COAGULATION WHICH IS S. AUREUS BACTERIA, THE TELLURITE TEST WAS POSITIVE AS WELL IT TURN BLAC WHICH INDICATES S. AUREUS, FOR THE CATALASE IT WAS POSITIVE BECAUSE IT BUBBLES WHICH IS S. AUREUS AGAIN, MY GRAM STAIN WAS PURPLE, CLUSTER SHAPE WHICH IS S.AUREUS, THE PROBLEM IS WHY I AM GETTING S. EPIDERMIS IN BLOOD AGAR, MANNITOL SALT AGAR AND DNASE ON MY RESULTS WHEN IT SUPPOSED TO BE S.AUREUS ALL THE WAY THROUGH PS: PLEASE HELP ME WITH THIS CONFUSION SINCE I HAVE TO DO A REPORT HOMEWORK FOR MY MICRO CLASS. THANK YOU SARA ...
CA and BiGGY agar are two chromogenic media which allow the presumptive differentiation of yeasts. CA contains various substrates for the enzymes of yeast species. It has been demonstrated that β-N-acetylgalactosaminidase which was produced by C. albicans enables the chromogenic substrates to be incorporated into the medium and the isolates of these species were seen as green colored colonies [16]. BiGGY agar contains bismuth sulphite and the growth on this medium produces brown to black colonies because of the extracellular reduction of bismuth sulphite to bismuth sulphide.. CA is reported to give green colonies of C. albicans and blue colonies of C. tropicalis. In this study 168 of 169 C. albicans isolates grew as distinctive light green colonies on CA, only one isolate was seen as off white colonies. Although we didnt have any C. dubliniensis isolates, differentiation of this species from C. albican s seems to be a problem in CA. Tintelnot et al [17] and Willinger et al [18] reported that ...
Five percent sheep blood and nicotinamide adenine dinucleotide may also be added when susceptibility testing is done on Streptococcus species. This type is also commonly used for susceptibility testing of Campylobacter.. It has a few properties that make it excellent for antibiotic use. First of all, it is a non-selective, non-differential medium. This means that almost all organisms plated on here will grow. Additionally, it contains starch. Starch is known to absorb toxins released from bacteria, so that they cannot interfere with the antibiotics. Second, it is a loose agar. This allows for better diffusion of the antibiotics than most other plates. A better diffusion leads to a truer zone of inhibition.. Mueller-Hinton agar was co-developed by John Howard Mueller and Jane Hinton at Harvard as a culture for gonococcus and meningococcus, who published the method in 1941.[2]. ...
ONDARZA, Mauricio A. Substitutions on the D-Galactose unit of agar polymers: Metabolic implications . Rev. biol. mar. oceanogr. [online]. 2007, vol.42, n.2, pp.201-204. ISSN 0718-1957. http://dx.doi.org/10.4067/S0718-19572007000200007.. Regarding macroalgae´s physiology, it is considered that substitutions on the L-Gal unit of agar polymers are representative of their biosynthesis. Studies performed within the Plant Cell Wall Polysaccharides Laboratory team have demonstrated the presence of 4-O-methyl-L-galactose in agar polymers from the rhodophycean thallophyte Gracilaria verrucosa and its linkage on the C-6 position of the D-Gal unit, which is also the site of O-methylation. Based on the 6-O-Me-Gal/ 4-O-Me-Gal ratio and the degree of substitution of D-Gal unit, a new approach for monitoring the characteristic events of agar metabolism was defined. Palabras clave : Agar; Gracilaria verrucosa; biosynthesis; O-methylation. ...
bioWORLD MacConkey Agar Plates w/ 1% Galactose & Chlor-12.5 - 1.7% Peptone. 0.3% Protease Peptone, 0.15% BileSalts #3, 0.5% NaCl, 1% Galactose, 0.003% Neutral Red, 0.0001% Crystal Violet, 12.5 �g/mL Chloramphenicol, 1.35% Agar
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Cell hybrids between malignant mouse hepatoma cells and normal rat fibroblasts with approximately one set of chromosomes from each parent exhibited remarkable karyotypic stability. Most chromosomes of both parents were retained even after prolonged culture in vitro. Normally, such hybrids showed suppression of the transformed phenotype and formed no colonies in soft agar. However, two hybrids, BS140 and BS181, formed a few colonies in soft agar when many cells were seeded, and also occasional foci of cells were detected piling up in monolayer cell cultures. We isolated soft agar colonies (a-subclones) and sub-clones from foci (h-subclones) of both hybrids, and, as a control, subclones of cells from random areas without foci of one hybrid (BS181 p-subclones). When tested for soft agar growth, cells from the a- and h-subclones of both BS140 and BS181 formed colonies at frequencies comparable to the malignant mouse hepatoma parent, whereas the control cells of the BS181 p-subclones (like the normal ...
Differential media are especially helpful when trying to identify a specific species. Bacteria can show widely different characteristics when grown in contact with various nutrients. A blood plate, one type of differential media, is often used to identify whether bacteria can digest blood cells. This is especially useful when diagnosing infection diseases. Streptococcus pneumoniae, a species that causes one type of pneumonia, can be identified partly thanks to its hemolytic activity, hemolysis being the breakdown of red blood cells. There are three different characteristic types of hemolysis that can be seen by growing bacteria on blood agar: alpha, beta, and gamma. Alpha hemolytic bacteria will only affect the medium that the colony is touching, turning the agar from red to green. Beta hemolytic bacteria cause a rather more dramatic change, removing the color from the media for some distance around each colony. Alpha hemolysis is only a partial digestion of the blood cells, while beta is a ...
Differential media are especially helpful when trying to identify a specific species. Bacteria can show widely different characteristics when grown in contact with various nutrients. A blood plate, one type of differential media, is often used to identify whether bacteria can digest blood cells. This is especially useful when diagnosing infection diseases. Streptococcus pneumoniae, a species that causes one type of pneumonia, can be identified partly thanks to its hemolytic activity, hemolysis being the breakdown of red blood cells. There are three different characteristic types of hemolysis that can be seen by growing bacteria on blood agar: alpha, beta, and gamma. Alpha hemolytic bacteria will only affect the medium that the colony is touching, turning the agar from red to green. Beta hemolytic bacteria cause a rather more dramatic change, removing the color from the media for some distance around each colony. Alpha hemolysis is only a partial digestion of the blood cells, while beta is a ...
Figure 1: Aerobic heterotrophic bacteria of 71 samples from the output water of dental chair units. The boxplot diagram shows the median, interquatrile range and outlier.. Abbreviations: R2A3572 = R2A agar at 35 C for 72 h; R2A35120 = R2A agar at 35 C for 120 h; R2A22120 = R2A agar at 22 C for 120 h; R2A22168 = R2A agar at 22 C for 168 h ...
... E. coli on Nutrient agar, Blood agar, MacConkey agar, EMB agar, m-ENDO agar, MHA, VRBA, CLED agar and Liquid media.
Get information, facts, and pictures about agar at Encyclopedia.com. Make research projects and school reports about agar easy with credible articles from our FREE, online encyclopedia and dictionary.
MacConkey, without Salt Agar Taylor Scientific 233120 233120 Difco™ Restricts the swarming of most Proteus sp., facilitating gram-negative bacilli isolation.
Agar project produces a portable and window system independent graphics toolkit for SDL and OpenGL In addition to a fully functional GUI toolkit a set of higherlevel graphics related libraries are also included in Agar For a complete
TGF-beta has the ability to transform rat fibroblasts, but it also induces neigbouring normal cells to eliminate newly arizing transformed cells. Therefore, the transforming effect of TGF-beta can only be demonstrated when it is added to rat fibroblasts sparsely seeded in soft agar. Combination of monolayer and soft agar cultures revealed the unexpected result that transformation of rat fibroblasts by TGF-beta acts on a distinct subpopulation rather than reaching the total population. As a consequence, the comparison of transformed cells with the parental untransformed random cell population is not adequate to define alterations acquired during the transformation process ...
Pour Plate Technique for Bacterial Enumeration RELATED PROTOCOLS: Bacterial Contamination of Milk and Meat , Yeast Plate Count PURPOSE: The pour plate technique can be used to determine the number of microbes/mL or microbes/gram in a specimen. It has the advantage of not requiring previously prepared plates, and is often used to assay bacterial contamination…
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A cell line designated MES-SA has been developed from a uterine sarcoma. Cells from the surgical tumor specimen were grown in a soft-agar clonogenic assay, with a relatively high plating efficiency of 0.5% and sensitivity to multiple drugs. Histologically, the surgical specimen and tumors developing after MES-SA inoculation into nude mice were identical, consisting of sheets of anaplastic sarcoma cells amid scant hyalinized stroma. The nonepithelial origin of this line was supported by ultrastructural analysis and negative mucin staining. Growth in monolayer was established by seeding colonies from soft agar into liquid media and has been maintained for over 21 months (greater than 100 passages), with a population-doubling time for the cell line of 22 hr. The MES-SA line readily forms colonies in soft agar with plating efficiencies ranging from 10 to 20%. Tumor cell inoculation s.c. into nude mice produces tumors within 2 to 3 weeks and subsequent tumor volume-doubling times of 7 to 10 days. ...
Grate 50g of potato, with skin, into a small sauce pot. Add 100ml of water and bring to a simmer for 30 minutes. Strain the potato extract through a fine-meshed wire strainer - if clearer plates are required additional filtration through cheesecloth or a coffee filter can also be performed - warning: this will be extremely slow and is generally unnecessary. Next, dissolve 2%/weight (2g/100ml) of dextrose (corn sugar) or another fermentable sugar like table sugar (sucrose) into the potato extract. Then slowly stir in 2%/weight agar, being careful to avoid agar lumps. Steralize and cast as described above/in the video ...
The identification of molecules involved in the mitogenic signaling and participating to the process of neoplastic transformation and progression has fostered the synthesis of novel agents able to selectively down-regulate such targets. PKAI seems to be one of such relevant targets suitable for therapeutic intervention, and antisense oligonucleotides against its RIα subunit have shown promising results in inhibiting human cancer cell growth in vitro and in vivo (9, 10, 11, 12 , 15) .. In the present study, we have demonstrated that HYB 165, a novel DNA/RNA hybrid MBO with improved pharmacokinetic and bioavailability properties in vivo, exhibits a dose-dependent inhibitory effect on soft agar growth of ZR-75-1 breast cancer cells.. We have also shown that HYB 165, but not its control oligo HYB 508, has a synergisitic inhibitory effect on ZR-75-1 colony formation when used in combination with docetaxel, a cytotoxic drug very active in breast cancer patients (26) . The antiproliferative activity ...
Need help with your Microbiology homework? Learners examine microscope images of blood agar and the various types of hemolysis that can be detected. A quiz completes the activity.
Definition of Novy and MacNeal blood agar. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
Bbl sabouraud dextrose agar bbl sabouraud dextrose agar bd. Sabouraud dextrose agar (agar dextrosa sabouraud) licuar sabouraud dextrose agar deeps en tubos a mediante ebullición en baño maría.* candida albicans.. medicine for yeast contamination facet results candida. I requerir un experto en este resolver mi problema. Medicinal drug for yeast contamination aspect effects garlic will help due to candida albicans are usually.. Que significa candidato a magister candida albicans en. Fermenti lattici consistent with candida quali; candida albicans sintomas how long does it take to kill the candida on average? Que significa candidato a magister these.. Candida albicans en agar dextrosa sabouraud. Candida albicans en agar dextrosa sabouraud if you have a susceptible immune device, remedy might be greater hard. Candida albicans en agar dextrosa sabouraud the.. Sabouraud agar wikipedia. Candida albicans in sabouraud agar. Trichophyton terrestre in sabouraud agar. Sabouraud agar normally includes 40 ...
Although we discussed agars origin briefly in the soy vanilla ice cream recipe, we didnt really cover some of its culinary uses.. A few interesting culinary quirks of agar: because it sets firmer and faster than gelatin, and at well above room temperature (122 degrees Fahrenheit, actually), using agar instead of gelatin can produce molds that will hold up to a fair bit of heat, where gelatin would melt. And if you remember, I mentioned in that ice cream post that before agar is heated, the carbohydrates that form its structure absorb water? Granulated agar can be a diet aid-it swells to three times its volume and makes you feel full with very few calories. (Its structure is about 80% dietary fiber, so if you go this route, you might want to cut back your Raisin Bran a little.). Powdered agar is a 1:1 substitute for gelatin, while granulated agar requires three times the amount (as the recipe here notes). From a box sold in Chicago, Illinois.. ...
In temperate and cold areas of the world, Yersinia enterocolitica is the third most common human bacterial enteropathogen (1). Although it mainly causes sporadic cases of gastroenteritis, outbreaks have been reported (2, 3). The species is subdivided into six biovars (1A, 1B, 2, 3, 4, and 5) and can also be separated into serogroups on the basis of the somatic (O) antigens, some of which are common to two or more biovars (2). However, the pathogenicity of the various serogroups of Y. enterocolitica is heterogeneous. When considering the 30 O serogroups defined by Wauters and coworkers (4, 5), most of the strains isolated from humans with diarrhea come from serogroups O:3, O:5,27, O:8 and O:9 (2). Despite the publication of a controversial report by Australian microbiologists 10 years ago (6), biovar 1A strains are generally considered to be nonpathogenic because they lack (in contrast to biovars 1B and 2 to 5) the pYV plasmid that is essential for bacterial virulence (2). This 70-kb ...
Members of the Pseudomonas family are commonly found in nature, some species are pathogenic for humans, as well as being resistant to multiple disinfectants. Various studies have revealed that benzalkonium chloride (BKC) has an inhibitory effect on many bacteria but it has no significant effect on Pseudomonas aeruginosa. Cetrimide agar medium is recommended for the isolation and enumeration of Ps. aeruginosa in food and environmental samples. However, there are claims that for some food factories and in particular the bottled water industry, the selectivity of this medium is not sufficient. The aim of the current research is the creation of a more selective medium for Ps. aeruginosa with BKC. A total of 28 isolates were isolated with Cetrimide agar from raw water samples and identified using biochemical tests and commercial identification kits. All the bacteria were inoculated in Cetrimide agar plates containing 0 - 625 μg/mL BKC. The Petri dishes were incubated at 37°C and 42°C for 24 h. The results
Background and aim: About 20% of non-pregnant women aged 15 to 55 harbour Candida albicans in the vagina .the aimed to determine the Characterization of Candida Species Isolated from women with Vulvovaginitis candidates (VVC) of reproductive ages. Methods: this descriptive study was conducted on 280 of who were selected for gathering samples by Purposive sampling based on their history and characteristics of vaginal discharges in 2009. Among these patients, 105 ones were diagnosed with candidiasis. The data were collected using demographic information form and disease symptoms. the species were differentiated using germ tube test, chrome agar test, and chlamidospore test. Data analysis was performed in SPSS V.16, using Descriptive Statistics Results: the prevalence of candida vaginitis was 9.3%.105 samples obtained from patients.. Chlamidospore was detected in 54.3% of the corn meal agar media. Besides, in chrome agar test, 41.9% of the samples turned into green representing candida albicans. In germ
ESBL/AmpC producing Enterobacteriaceae have been reported worldwide amongst isolates obtained from humans, food-producing animals, companion animals and environmental sources. However, data on prevalence of fecal carriage of ESBL/AmpC producing Enterobacteriaceae in healthy companion animals is limited. This pilot study describes the prevalence of ESBL/AmpC encoding genes in healthy cats and dogs, and cats and dogs with diarrhea. Twenty fecal samples of each group were cultured on MacConkey agar supplemented with 1 mg/L cefotaxime and in LB-enrichment broth supplemented with 1 mg/L cefotaxime, which was subsequently inoculated on MacConkey agar supplemented with 1 mg/L cefotaxime. ESBL/AmpC genes were identified using the Check-Points CT103 micro array kit and subsequently by sequencing analysis. Chromosomal ampC promoter mutations were detected by PCR and sequencing analysis. From the healthy and diarrheic dogs, respectively 45% and 55% were positive for E. coli with reduced susceptibility for
The ulcers of successive patients attending EITC, Chittagong, with suppurative keratitis were scraped for microscopy and culture, after the history had been taken and the visual acuity measured. The specimens were examined microscopically as a wet mount in 10% potassium hydroxide and as a heat fixed mount with Gram stain. It was necessary that fungal hyphal elements were observed for the patients to be recruited to the trial. Corneal scrapings were cultured at 28°C in Sabourauds dextrose agar (SDA), chocolate agar, and blood agar media. If there was no growth on the 14th day the cultures were discarded. The sensitivity tests for fungi were done by the well diffusion method, filling 7 mm wells punched in SDA in a plate with 0.2% chlorhexidine, 2.5% natamycin, and 1% econazole. The diameters of the zones of inhibition were taken and 7 mm subtracted.. Exclusion criteria were patients with only one eye; those with diabetes mellitus; those with polymicrobial infections; those who were unwilling to ...
Staphylococcus aureus is an important opportunistic pathogen of humans and animals and a leading global cause of foodborne disease (1). Poultry as a potential reservoir for the zoonotic transmission of drug-resistant S. aureus to humans has been well documented (2). Despite the apparent threat, the genomics of antibiotic-resistant S. aureus in food animals appear to be neglected in Africa (3, 4). Toward this end, S. aureus SA9, isolated from a slaughterhouse chicken carcass in South Africa, was subjected to whole-genome sequencing (WGS). Antimicrobial susceptibility testing utilizing the broth microdilution method revealed its multidrug-resistant profile.. Strain SA9 was isolated and confirmed using HiCrome Aureus agar base (HiMedia Laboratories, Mumbai, India) and an API Staph kit (bioMérieux, Marcy-lEtoile, France), respectively. It was streaked onto Mueller-Hinton agar (MHA) plates and incubated at 37°C for 24 h. Following incubation, genomic DNA was extracted from 1 CFU of a visibly pure ...