The O26 serogroup of enteropathogenic Escherichia coli (EPEC) is one of the serogroups most frequently implicated in infant diarrhea and is also common among enterohemorrhagic E. coli (EHEC) strains. the most common O26 strains belong to EPEC/EHEC serotype O26:H11 and are generally Shiga toxin (Stx) positive. Stx-negative E. coli strains that are negative for the EPEC EAF plasmid and bundle-forming pilus (Bfp) are classified as atypical EPEC. Here, we report a novel adhesin present in an stx-negative bfpA-negative atypical EPEC O26:H11 strain isolated from an infant with diarrhea. A cloned 15-kb genomic region from this strain, designated the locus for diffuse adherence (lda), confers diffuse adherence on HEp-2 cells when expressed in E. coli K-12. Sequence analysis of lda revealed a G+C content of 46.8% and 15 open reading frames sharing homology with the E. coli K88 fae and CS31A clp fimbrial operons. the lda region is part of a putative 26-kb genomic island inserted into the proP gene of the ...

Enteropathogenic Escherichia coli-like E. coli strains belonging to serovar O103:K-:H2 and rhamnose-negative biotypes are highly pathogenic diarrhea-inducing strains for weaned European rabbits. We describe here the cloning and sequencing of the major subunit gene of a new fimbrial adhesin, adhesive factor/rabbit 2 (AF/R2), which confers on these strains the ability to attach to rabbit enterocytes and to HeLa cells in a diffuse manner and which is associated with in vivo virulence. The chromosomal operon that encodes functional AF/R2 has been cloned from strain B10. The major subunit gene afr2G, as well as an adjacent open reading frame, afr2H, has been sequenced. The Afr2G protein shows homologies with FaeG and ClpG, which are the respective major subunits of fimbrial adhesin K88 (F4) and afimbrial adhesin CS31A. Plasmid carrying the operon transcomplements an AF/R2-negative TnphoA mutant for its ability to express AF/R2. As a whole, AF/R2 is a new member of the E. coli K88 adhesin family which ...

Background: The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. We have previously reported the use of the AIDA-I autotransport system to express the Salmonella enterica serovar Enteritidis proteins SefA and H: gm. The SefA protein was successfully exposed to the medium, but the orientation of H:gm in the outer membrane could not be determined due to proteolytic cleavage of the N-terminal detection-tag. The goal of the present work was therefore to construct a vector containing elements that facilitates analysis of surface expression, especially for proteins that are sensitive to proteolysis or otherwise difficult to express. Results: The surface expression system pAIDA1 was created with two detection tags flanking the passenger protein. Successful expression of SefA and H:gm on the surface of E. coli was confirmed with fluorescently labeled antibodies specific for the N-terminal His(6)-tag and the ...

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S fimbrial adhesins (Sfa) enable pathogenic Escherichia coli strains to bind to sialic acid-containing eucaryotic receptor molecules. In order to determine the influence of culture conditions on the expression of the sfa determinant in a wild-type strain, we fused the gene lacZ, coding for the enzyme beta-galactosidase, to the sfaA gene, responsible for the major protein subunit of S fimbriae. By using a plasmid which carries an R6K origin, the sfaA-lac hybrid construct was site-specifically integrated into the chromosome of the uropathogenic E. coli strain 536WT. The expression of lacZ, which was under the control of the sfa wild-type promoters, was now equivalent to the sfa expression of strain 536WT. With the help of this particular wild-type construct, it was demonstrated that the sfa determinant is better expressed on solid media than in liquid broth. The growth rate had a strong influence on Sfa expression under aerobic but not under anaerobic conditions. Production of Sfa was further ...

Autotransporter proteins are synthesized as multidomain proteins containing all structural requirements for the transport to the cell surface. They contain an N-terminal signal peptide typical for the Sec pathway, enabling export across the inner membrane. After truncation of the signal peptide in the periplasm, the C-terminal domain folds into the outer membrane as a porine-like structure, a so called β-barrel. The passenger domain can be efficiently translocated to the extracellular milieu by this structure. Recombinant passengers can be transported to the surface by simple insertion of the corresponding coding sequence into a distinct position of the precursor gene.. .reading-box-container-2 .element-bottomshadow:before,.reading-box-container-2 .element-bottomshadow:after{opacity:0.7;} ...

Papež Jan Pavel II. se již poněkolikáté vrátil do nemocnice, vážně nemocen. I pro nekatolíky, jako jsem já, jde o příležitost k zamyšlení, o co svět přijde, až papežovo tělo nakonec podlehne svým neduhům. Obraz, který vyvstane, je směsicí barev. Pro ty z nás, kdo považují pád komunismu z roku 1989 za zásadní událost dějin dvacátého století, je papež Jan Pavel hrdina. V Polsku byl ohniskem všech aktivit občanské společnosti. Zatímco v ostatních zemích, nejsilněji v Rumunsku, ale i v tehdejším Československu a v Maďarsku, vedle komunismu bylo vakuum nebo přinejlepším několik izolovaných organizací občanské společnosti, Polsko mělo alternativní zdroj legitimity. Tehdejší krakovský kardinál Karol Wojtyla byl jeho nejpůsobivějším představitelem, před svým zvolením i po něm. Zvolení kardinála Wojtyly papežem tak svým významem přesahovalo hranice církve. On sám vlastně ztotožňování své církve s občanskou ...

When you exercise heavily, you lose water and salt in your sweat. One good source is Emergen-C®. Emergen-C has an advantage over water because it is packed with the potassium, magnesium, calcium, sodium and sugar to provide electrolyte replenishment and energy during workouts Athletes can stave off fatigue longer if they fortify their water with…

The S fimbrial adhesin (sfa) determinant of E. coli comprises nine genes situated on a stretch of 7.9 kilobases (kb) DNA. Here… Expand ...

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Pentadbiran Makanan dan Dadah AS (FDA) telah meluluskan permintaan ASC Therapeutics untuk membuka percubaan klinikal di AS mengenai keselamatan dan keberkesanan awal ASC618, terapi gen generasi kedua untuk hemofilia A. Percubaan Fasa 1/2 (NCT04676048 ), yang akan bermula pada bulan ini, akan menguji terapi sekali sahaja sehingga 12 orang dewasa dengan hemofilia sederhana hingga teruk A. Maklumat hubungan boleh didapati di sini; laman web pendaftaran belum didedahkan.

Moonens, K., J. Bouckaert, A. Coddens, T. Tran, S. Panjikar, M. De Kerpel, E. Cox, H. Remaut, and H. De Greve, Structural insight in histo-blood group binding by the F18 fimbrial adhesin FedF., Mol Microbiol, vol. 86, issue 1, pp. 82-95, 2012 Oct. ...

Maumivu ya nyonga siyo ugonjwa bali ni dalili ya uwepo wa tatizo la kiafya. Maumivu haya yanaweza kuwa ya muda mfupi au ya muda mrefu na hutokea kweye nyonga

Michaelis, W. , Seifert, R. , Nauhaus, K. , Treude, T. , Thiel, V. , Blumenberg, M. , Knittel, K. , Gieseke, A. , Peterknecht, K. , Pape, T. , Boetius, A. , Amann, R. , Jorgensen, B. B. , Widdel, F. , Peckmann, J. , Pimenov, N. V. and Gulin, M. B. (2002 ...

Nitsch, J.; Pregger, T.; Naegler, T.; Heide, D.; Scholz, Y.; Luca de Tena, D.; Trieb, F.; Nienhaus, K.; Gerhardt, N.; Sterner, M.; Trost, T.; von Oehsen, A.; Schwinn, R.; Pape, C.; Hahn, H.; Wickert, M.; Wenzel, B.. ...

TY - JOUR. T1 - Aggregative adherence fimbriae II of enteroaggregative Escherichia coli are required for adherence and barrier disruption during infection of human colonoids. AU - Gonyar, Laura A.. AU - Smith, Rachel M.. AU - Giron, Jorge A.. AU - Zachos, Nicholas C.. AU - Ruiz-Perez, Fernando. AU - Nataro, James P.. N1 - Funding Information: This work was supported by the National Institutes of Health (P01-AI125181) to J.P.N. and by the Hartwell Foundation through a postdoctoral research fellowship to L.A.G. We acknowledge the Integrated Physiology Core of the Hopkins Conte Digestive Disease Basic and Translational Research Core Center (NIH P30 DK-089502).. PY - 2020/9. Y1 - 2020/9. N2 - Symptomatic and asymptomatic infection with the diarrheal pathogen enteroaggregative Escherichia coli (EAEC) is associated with growth faltering in children in developing settings. The mechanism of this association is unknown, emphasizing a need for better understanding of the interactions between EAEC and the ...

We recently reported that the type 1-fimbriated Escherichia coli strains CSH-50 and HB101(pPKL4), both K-12 derivatives, have different patterns of adhesion to yeast mannan, human plasma fibronectin, and fibronectin derivatives, suggesting functional heterogeneity of type 1 fimbriae. In this report, we provide evidence that this functional heterogeneity is due to variations in the fimH genes. We also investigated functional heterogeneity among clinical isolates and whether variation in fimH genes accounts for differences in receptor specificity. Twelve isolates obtained from human urine were tested for their ability to adhere to mannan, fibronectin, periodate-treated fibronectin, and a synthetic peptide copying the 30 amino-terminal residues of fibronectin. CSH-50 and HB101(pPKL4) were tested for comparison. Selected isolates were also tested for adhesion to purified fragments spanning the entire fibronectin molecule. Three distinct functional classes, designated M, MF, and MFP, were observed. ...

Enteroaggregative Escherichia coli (EAEC) is a recognized cause of acute diarrhea among both children and adults worldwide. EAEC strains are characterized by the presence of aggregative adherence fimbriae (AAF), which play a key role in pathogenesis by mediating attachment to the intestinal mucosa and by triggering host inflammatory responses. The aggregative adherence fimbria II (AAF/II) is the most important adherence factor of EAEC prototype strain 042 (EAEC042) to intestinal cells. Multiple receptors for AAF/II on epithelial cells have been identified including the transmembrane signaling mucin Muc1. This protocol describes a method to measure adherence of EAEC strains to HEK293 cells expressing the Muc1 glycoprotein.

The adhesin involved in diffuse adherence (AIDA) is an autotransporter protein that confers the diffuse adherence phenotype to certain diarrheagenic Escherichia coli strains. It consists of a 49 amino acid signal peptide, a 797 amino acid passenger domain, and a 440 amino acid beta-domain integrated into the outer membrane. The beta-domain consists of two parts: the beta(1)-domain, which is predicted to form two beta-strands on the bacterial cell surface, and the beta(2)-domain, which constitutes the transmembrane domain. We have previously shown that the beta-domain can be folded from the urea-denatured state when bound to a nickel column during purification. It has not been possible to achieve proper refolding of the beta-domain in solution; instead, a misfolded state C is formed. Here, we characterize this misfolded state in greater detail, showing that despite being misfolded, C can be analyzed as a conventional conformational state, with cooperative unfolding in urea and SDS as well as ...

Surface expression has attracted much recent interest, and it has been suggested for a variety of applications. Two such applications are whole-cell biocatalysis and the creation of live vaccines. For successful implementation of these applications there is a need for flexible surface expression systems that can yield a high level of expression with a variety of recombinant fusion proteins. The aim of this work was thus to create a surface expression system that would fulfil these requirements.. A novel surface expression system based on the AIDA-I autotransporter was created with the key qualities being are good, protein-independent detection of the expression through the presence of two epitope tags flanking the recombinant protein, and full modularity of the different components of the expression cassette. To evaluate the flexibility of this construct, 8 different model proteins with potential use as live-vaccines or biocatalysts were expressed and their surface expression levels were ...

Background & Objective: Patient satisfaction is an indicator of the health services that is increasingly important in today's competitive world and perhaps the simplest method for evaluation of family physician program. This study aimed to determine the level of satisfaction of service recipients in urban areas in Fasa, Iran, with ...

Dive into the research topics of Protease activity, secretion, cell entry, cytotoxicity, and cellular targets of secreted autotransporter toxin of uropathogenic Escherichia coli. Together they form a unique fingerprint. ...

The ability of Escherichia coli to express the K88 fimbrial adhesin was satisfactorily indicated by the combined techniques of ELISA, haemagglutination and latex agglutination. Detection of expression by electron microscopy and the ability to metabolize raffinose were unsuitable. Quantitative expression of the K88 adhesin was determined by ELISA. Expression was found to vary according to the E.coli strain examined, media type and form. In general it was found that the total amount was greater, while the amount/cfu was less on agar than in broth cultures. Expression of the K88 adhesin during unshaken batch culture was related to the growth rate and was maximal during late logarithmic to early stationary phase. A combination of heat extraction, ammonium sulphate and isoelectric precipitation was found suitable for both large and small scale preparation of purified K88ab adhesin. Extraction of the K88 adhesin was sensitive to pH and it was postulated that this may affect the site of colonisation of ...

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Define adhesin: any of various specialized molecular components (such as proteins) on the surface of a bacterial cell that… - adhesin in a sentence

I work on an uropathogenic strain of E.coli. When I artificially express from a plasmid one of the transcriptional regulator, it results in secretion of a protein factor in the media, which has interesting biological roles. My aim is to purify this unknown factor present in the growth media and to identify it by Mass Spec ...

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The most important they really understanding in any situation they faced during the day. Pinggan xde di meja kek,Saya yg tiba2 senggugut hari majlis,Saya dn suami dtg lambat di lokasi outdoor.Agaknya mungkin sejam jugak mereka menunggu. *maafkan saye yee...* Tetapi hasil kerja yg mereka berikan tetap berkualiti. Ada umph laaa..:P..And if u like any pose tat u have seen before,atau tiba2 teringin nak bergambar ats pokok ke..just tell them..they will fulfill your wish..*err..boleh ke abg wan?..heheh* ...

Hi guys, little question, on pape 33 of DX8 manual under gyro it says, The DX8 displays gyro gain values as N for Normal gain and a T for tail

隨著民眾的生活水準提升與對自己外在觀感的逐漸重視，美妝保養品由過去的奢侈品轉變為現在大部分民眾生活中的必需品，加上近幾年來消費者環保意識的提升，對於環境永續議題逐漸重視，促使各個產業爭相發展綠色相關產品，綠色美妝保養品也在此趨勢下開始發展。據此，本研究將針對台灣消費者對綠色美妝保養品之相關消費者行為進行探究 ...

The main causative agent of human urinary tract infections is the uropathogenic Escherichia coli (UPEC) pathotype. It may cause disease due to its ability to express a number of bacterial virulence factors. Fimbrial adhesins are particularly important for the initial establishment of infection in the urinary tract. The fimbriae are hair-like structures protruding from the bacterial cell and by attaching to specific receptors in the urinary tract they mediate adherence to different cell types, allowing the bacteria to resist the shear forces from urine flow. The UPEC strains generally carry multiple determinants for fimbrial adhesins. Previous studies have indicated that there is a co-regulation between different fimbrial genes and one factor that has been implicated in this is the PapB protein, acting as a transcriptional regulator of P-fimbrial expression. The PapB protein can be regarded as the prototype of a family of fimbrial regulators that show high homology between different fimbrial ...

Escherichia coli colonizes the human intestine shortly after birth, with most strains engaging in a commensal relationship. However, some E. coli strains have evolved toward acquiring genetic traits associated with virulence. Currently, five categories of enteroadherent E. coli strains are well-recognized, and are classified in regard to expressed adhesins and the strategy used during the colonization. The high morbidity associated with diarrhea has motivated investigations focusing on E. coli adhesins, as well on factors that inhibit bacterial adherence. Breastfeeding has proved to be the most effective strategy for preventing diarrhea in children. Aside from the immunoglobulin content, glycocompounds and oligosaccharides in breast milk play a critical role in the innate immunity against diarrheagenic E. coli strains. This review summarizes the colonization factors and virulence strategies exploited by diarrheagenic E. coli strains, addressing the inhibitory effects that oligosaccharides and

Fig. 2. Biofilm formation by E. coli cells expressing FimH variants. Each FimH variant is indicated by its expression plasmid. (A) Biofilm formation on a microtiter plate surface by cells expressing the K-12 fimH allele when grown in LB under static conditions. (B) Biofilm formation on a microtiter plate surface by cells expressing FimH variants when grown in LB under HDF conditions. Note that no biofilm is produced by the K-12fimH allele (pMAS1) under these conditions. (C) Quantification of biofilm formation as determined by the amount of crystal violet staining for each of the FimH variants. All experiments were performed in the presence and absence of methyl-α-d-mannopyranoside (α mm). Results are expressed as the average of four independent experiments (± standard deviation). ...

The production of fimbrial adhesins K99 and F41 by enterotoxigenic Escherichia coli has been measured in steady-state chemostat experiments at various specific growth rates (microseconds) and in a recycling fermentor across a range of mu values falling to less than 0.004 h-1. It has been demonstrated that the production of K99 and F41 fimbriae is correlated with mu both in aerobic and anaerobic chemostat experiments. A significant production of fimbriae was only detected at mu values higher than 0.2 h-1. This behavior was further examined by culturing the bacteria in a recycling fermentor with complete biomass retention. It could be shown that the production of K99 and F41 fimbriae only occurred during balanced growth, with a high biomass yield at mu values higher than 0.04 h-1 corresponding to mass doubling times (td) of less than 17 h. The production of both fimbriae halted during balanced growth with a lower biomass yield (at mu values between 0.012 and 0.04 h-1 corresponding to td values ...

Urinary tract infections (UTIs) result in $1.6 billion in medical expenditures in the United States each year (1), with uropathogenic strains of Escherichia coli (UPEC) accounting for 70 to 95% of all UTIs (2). With the advance of multi-drug-resistant UPEC (3), it is important to determine the pathogenic mechanisms of UPEC. In animal models, UPEC pathogenesis initiates with bacterial binding of superficial bladder epithelial cells via the adhesin FimH at the tips of bacterially expressed type 1 pili (4). Initial colonization events activate inflammatory and apoptotic cascades in the epithelium, which is normally inert and only turns over every 6 to 12 months (5). Bladder epithelial cells respond to invading bacteria in part by recognizing bacterial lipopolysaccharide (LPS) via the Toll-like receptor 4 (TLR-4)-CD14 pathway, which results in strong neutrophil influx into the bladder (6). In addition, FimH-mediated interactions with the bladder epithelium stimulate exfoliation of superficial ...

UPEC utilizes complex mechanisms to subvert innate defenses to persist and cause disease. The ability of UPEC to invade into superficial cells of the bladder has been shown to be a critical mechanism in the ability of UPEC to establish a persistent infection (8, 12, 14, 15). Upon entry, UPEC diverts itself into the cytoplasm by an unknown mechanism that depends on the FimH adhesin present at the tips of type 1 pili (7). Previous light and electron microscopic studies identified loose intracellular collections of bacteria at early time points (≈6 h) in the superficial umbrella cells of the bladder, which were termed bacterial factories (8). In addition, at later time points (24 h) intracellular biofilm-like communities were observed that created a bulging appearance on the luminal surface of the bladder that were termed pods (15). Based on real-time fluorescence microscopy studies presented here, we discovered that these various structures are formed as part of a continuous developmental and ...

The paper in Nature Microbiology can be found here. The work presented in this paper was the result of a strong collaboration between the laboratories of Dr. Scott J. Hultgren and Dr. Peng Yuan at Washington University in St. Louis. Chaperone-usher pathway (CUP) pili are extracellular proteinaceous fibers ubiquitously found on Gram-negative bacteria. Type 1 and P pili are produced by uropathogenic strains of E. coli and are tipped with the FimH and PapG adhesins, respectively, to mediate host and tissue tropism to the bladder and kidney. During pilus assembly hundreds of individual pilus subunits called pilins are first exported across the inner membrane to the periplasm. Chaperone-pilin complexes are then guided to an outer membrane (OM) nanomachine called the usher, which catalyzes subunit-subunit interactions. The usher contains five functional domains: a 24-stranded transmembrane β-barrel domain, a β-sandwich plug domain that resides in the pore of the TD in the apo-usher, an ...

Previous reports showed successful applications of FimH antagonists in vitro and in vivo. The antagonists reduced bacterial attachment to cells and surfaces and decreased bladder infections in a UTI mouse model. Within this thesis, FimH antagonists from our group were screened for their minimal anti-adhesive concentration (MAC90) using an in vitro cell infection assay. The minimal therapeutic concentration was analyzed in the context of the pharmacokinetic (PK) performance of individual antagonists. It could be shown, that a preventive application and the resulting peak concentration of a FimH antagonist in the urine relative to the MAC90 value is predictive for positive treatment outcome. Guided by this finding, several treatment regimens, including combination therapies with antibiotics, were successfully applied to reduce bladder infections in an experimental mouse model by up to three orders of magnitude. Furthermore, FimH antagonists were effective against catheter-associated UTI (CAUTI), ...

1O9Z: The Fimbrial Adhesin F17-G of Enterotoxigenic Escherichia Coli Has an Immunoglobulin-Like Lectin Domain that Binds N-Acetylglucosamine

K. John Pasi, MB, ChB, PhD, FRCPath, FRCPCH, anajadili dondoo iliyopewa jina la hivi karibuni Utafiti wa Ufuataji wa Miaka Nne wa Binadamu wa Utafiti wa Kudumu wa Tiba na Usalama: Tiba ya Jeni ya AAV Pamoja na Valoctocogene Roxaparvovec kwa Ukali Haemophilia A

Pape NDiasse Sarr is a footballer from Senegal and you can read his player profile to know his nationality, date of birth, height, weight. For statistics on his career transfers, total goals scored and matches played.

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1 Sequence alignment [w] Consider the strings APPLE and PAPE over the alphabet E = {A,E,L,P} and a penalty matrix P: A E L P A 1 E 1 1 L. 2 1 2 Compute the sequ

Vanwetswinkel, S., A. Volkov, Y. G. J. Sterckx, A. Garcia-Pino, L. Buts, W. F. Vranken, J. Bouckaert, R. Roy, L. Wyns, and N. A. J. van Nuland, Study of the structural and dynamic effects in the FimH adhesin upon α-d-heptyl mannose binding., J Med Chem, vol. 57, issue 4, pp. 1416-27, 2014 Feb 27. ...

The clinical presentation, course, and treatment of diarrhea depend on its cause. Are you familiar with common conditions associated with acute or chronic diarrhea? Test yourself with this short quiz.

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Berichte zur Polar- und Meeresforschung (Reports on Polar and Marine Research), Bremerhaven, Alfred Wegener Institute for Polar and Marine Research, 473 , 103 p ...

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saya students uitm arau, menyewa di taman sri wang fasa 3, lorong 28.. betul2 depan sawah.. saya merupakan mangsa terbaru gigitan cinta pakcik charlie nih,, iaitu malam semalam. mmg betul kate kamu sebab saye tido x tutup lampu. sedih kot, minggu depan nak raye dah.. kene kat muke pulak tuh. masalah pada saya, saye x kenal rupe pkcik charlie nih. kalo jumpe, ku gigit2 balik siap kamu!! ...

Great value bright Box files, high quality board with a paper cover. Flush fitting lid with a strong press-button catch. Interior lock-spring secures loose pape

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Saya baru je balik kerja ni. Now tgh tunggu mak, abah n adik2 sampai. Mereka bertolak tghhari td dr kedah. Skrg (6.00pm) dah ada di behrang. Menurut mak, ujan lebat sgt. Dianggarkan akan tiba lepas maghrib.. X kisahlah, asalkan semuanya selamat... ...

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