The O26 serogroup of enteropathogenic Escherichia coli (EPEC) is one of the serogroups most frequently implicated in infant diarrhea and is also common among enterohemorrhagic E. coli (EHEC) strains. the most common O26 strains belong to EPEC/EHEC serotype O26:H11 and are generally Shiga toxin (Stx) positive. Stx-negative E. coli strains that are negative for the EPEC EAF plasmid and bundle-forming pilus (Bfp) are classified as atypical EPEC. Here, we report a novel adhesin present in an stx-negative bfpA-negative atypical EPEC O26:H11 strain isolated from an infant with diarrhea. A cloned 15-kb genomic region from this strain, designated the locus for diffuse adherence (lda), confers diffuse adherence on HEp-2 cells when expressed in E. coli K-12. Sequence analysis of lda revealed a G+C content of 46.8% and 15 open reading frames sharing homology with the E. coli K88 fae and CS31A clp fimbrial operons. the lda region is part of a putative 26-kb genomic island inserted into the proP gene of the ...
Background: The discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of Escherichia coli. We have previously reported the use of the AIDA-I autotransport system to express the Salmonella enterica serovar Enteritidis proteins SefA and H: gm. The SefA protein was successfully exposed to the medium, but the orientation of H:gm in the outer membrane could not be determined due to proteolytic cleavage of the N-terminal detection-tag. The goal of the present work was therefore to construct a vector containing elements that facilitates analysis of surface expression, especially for proteins that are sensitive to proteolysis or otherwise difficult to express. Results: The surface expression system pAIDA1 was created with two detection tags flanking the passenger protein. Successful expression of SefA and H:gm on the surface of E. coli was confirmed with fluorescently labeled antibodies specific for the N-terminal His(6)-tag and the ...
If screening applications are viable for the success of your R&D efforts, a very unique approach can help:. Our proprietary technologies MATE and Autodisplay enable the development of cost-efficient high-throughput screening tools for the identification of binding partners such as inhibitors, activators, biomarkers or autoantigens. The use of fluorescence marked libraries speeds up the process significantly, when coupled to fluorescence activated cell sorting (FACS).. Find out how our custom-tailored products and services can assist in bringing your R&D processes forward ...
S fimbrial adhesins (Sfa) enable pathogenic Escherichia coli strains to bind to sialic acid-containing eucaryotic receptor molecules. In order to determine the influence of culture conditions on the expression of the sfa determinant in a wild-type strain, we fused the gene lacZ, coding for the enzyme beta-galactosidase, to the sfaA gene, responsible for the major protein subunit of S fimbriae. By using a plasmid which carries an R6K origin, the sfaA-lac hybrid construct was site-specifically integrated into the chromosome of the uropathogenic E. coli strain 536WT. The expression of lacZ, which was under the control of the sfa wild-type promoters, was now equivalent to the sfa expression of strain 536WT. With the help of this particular wild-type construct, it was demonstrated that the sfa determinant is better expressed on solid media than in liquid broth. The growth rate had a strong influence on Sfa expression under aerobic but not under anaerobic conditions. Production of Sfa was further ...
Autotransporter proteins are synthesized as multidomain proteins containing all structural requirements for the transport to the cell surface. They contain an N-terminal signal peptide typical for the Sec pathway, enabling export across the inner membrane. After truncation of the signal peptide in the periplasm, the C-terminal domain folds into the outer membrane as a porine-like structure, a so called β-barrel. The passenger domain can be efficiently translocated to the extracellular milieu by this structure. Recombinant passengers can be transported to the surface by simple insertion of the corresponding coding sequence into a distinct position of the precursor gene.. .reading-box-container-2 .element-bottomshadow:before,.reading-box-container-2 .element-bottomshadow:after{opacity:0.7;} ...
Papež Jan Pavel II. se již poněkolikáté vrátil do nemocnice, vážně nemocen. I pro nekatolíky, jako jsem já, jde o příležitost k zamyšlení, o co svět přijde, až papežovo tělo nakonec podlehne svým neduhům. Obraz, který vyvstane, je směsicí barev. Pro ty z nás, kdo považují pád komunismu z roku 1989 za zásadní událost dějin dvacátého století, je papež Jan Pavel hrdina. V Polsku byl ohniskem všech aktivit občanské společnosti. Zatímco v ostatních zemích, nejsilněji v Rumunsku, ale i v tehdejším Československu a v Maďarsku, vedle komunismu bylo vakuum nebo přinejlepším několik izolovaných organizací občanské společnosti, Polsko mělo alternativní zdroj legitimity. Tehdejší krakovský kardinál Karol Wojtyla byl jeho nejpůsobivějším představitelem, před svým zvolením i po něm. Zvolení kardinála Wojtyly papežem tak svým významem přesahovalo hranice církve. On sám vlastně ztotožňování své církve s občanskou ...
When you exercise heavily, you lose water and salt in your sweat. One good source is Emergen-C®. Emergen-C has an advantage over water because it is packed with the potassium, magnesium, calcium, sodium and sugar to provide electrolyte replenishment and energy during workouts Athletes can stave off fatigue longer if they fortify their water with…
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Moonens, K., J. Bouckaert, A. Coddens, T. Tran, S. Panjikar, M. De Kerpel, E. Cox, H. Remaut, and H. De Greve, Structural insight in histo-blood group binding by the F18 fimbrial adhesin FedF., Mol Microbiol, vol. 86, issue 1, pp. 82-95, 2012 Oct. ...
Michaelis, W. , Seifert, R. , Nauhaus, K. , Treude, T. , Thiel, V. , Blumenberg, M. , Knittel, K. , Gieseke, A. , Peterknecht, K. , Pape, T. , Boetius, A. , Amann, R. , Jorgensen, B. B. , Widdel, F. , Peckmann, J. , Pimenov, N. V. and Gulin, M. B. (2002 ...
Nitsch, J.; Pregger, T.; Naegler, T.; Heide, D.; Scholz, Y.; Luca de Tena, D.; Trieb, F.; Nienhaus, K.; Gerhardt, N.; Sterner, M.; Trost, T.; von Oehsen, A.; Schwinn, R.; Pape, C.; Hahn, H.; Wickert, M.; Wenzel, B.. ...
We recently reported that the type 1-fimbriated Escherichia coli strains CSH-50 and HB101(pPKL4), both K-12 derivatives, have different patterns of adhesion to yeast mannan, human plasma fibronectin, and fibronectin derivatives, suggesting functional heterogeneity of type 1 fimbriae. In this report, we provide evidence that this functional heterogeneity is due to variations in the fimH genes. We also investigated functional heterogeneity among clinical isolates and whether variation in fimH genes accounts for differences in receptor specificity. Twelve isolates obtained from human urine were tested for their ability to adhere to mannan, fibronectin, periodate-treated fibronectin, and a synthetic peptide copying the 30 amino-terminal residues of fibronectin. CSH-50 and HB101(pPKL4) were tested for comparison. Selected isolates were also tested for adhesion to purified fragments spanning the entire fibronectin molecule. Three distinct functional classes, designated M, MF, and MFP, were observed. ...
The adhesin involved in diffuse adherence (AIDA) is an autotransporter protein that confers the diffuse adherence phenotype to certain diarrheagenic Escherichia coli strains. It consists of a 49 amino acid signal peptide, a 797 amino acid passenger domain, and a 440 amino acid beta-domain integrated into the outer membrane. The beta-domain consists of two parts: the beta(1)-domain, which is predicted to form two beta-strands on the bacterial cell surface, and the beta(2)-domain, which constitutes the transmembrane domain. We have previously shown that the beta-domain can be folded from the urea-denatured state when bound to a nickel column during purification. It has not been possible to achieve proper refolding of the beta-domain in solution; instead, a misfolded state C is formed. Here, we characterize this misfolded state in greater detail, showing that despite being misfolded, C can be analyzed as a conventional conformational state, with cooperative unfolding in urea and SDS as well as ...
Surface expression has attracted much recent interest, and it has been suggested for a variety of applications. Two such applications are whole-cell biocatalysis and the creation of live vaccines. For successful implementation of these applications there is a need for flexible surface expression systems that can yield a high level of expression with a variety of recombinant fusion proteins. The aim of this work was thus to create a surface expression system that would fulfil these requirements.. A novel surface expression system based on the AIDA-I autotransporter was created with the key qualities being are good, protein-independent detection of the expression through the presence of two epitope tags flanking the recombinant protein, and full modularity of the different components of the expression cassette. To evaluate the flexibility of this construct, 8 different model proteins with potential use as live-vaccines or biocatalysts were expressed and their surface expression levels were ...
Background & Objective: Patient satisfaction is an indicator of the health services that is increasingly important in today's competitive world and perhaps the simplest method for evaluation of family physician program. This study aimed to determine the level of satisfaction of service recipients in urban areas in Fasa, Iran, with ...
The ability of Escherichia coli to express the K88 fimbrial adhesin was satisfactorily indicated by the combined techniques of ELISA, haemagglutination and latex agglutination. Detection of expression by electron microscopy and the ability to metabolize raffinose were unsuitable. Quantitative expression of the K88 adhesin was determined by ELISA. Expression was found to vary according to the E.coli strain examined, media type and form. In general it was found that the total amount was greater, while the amount/cfu was less on agar than in broth cultures. Expression of the K88 adhesin during unshaken batch culture was related to the growth rate and was maximal during late logarithmic to early stationary phase. A combination of heat extraction, ammonium sulphate and isoelectric precipitation was found suitable for both large and small scale preparation of purified K88ab adhesin. Extraction of the K88 adhesin was sensitive to pH and it was postulated that this may affect the site of colonisation of ...
Define adhesin: any of various specialized molecular components (such as proteins) on the surface of a bacterial cell that… - adhesin in a sentence
I work on an uropathogenic strain of E.coli. When I artificially express from a plasmid one of the transcriptional regulator, it results in secretion of a protein factor in the media, which has interesting biological roles. My aim is to purify this unknown factor present in the growth media and to identify it by Mass Spec ...
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The most important they really understanding in any situation they faced during the day. Pinggan xde di meja kek,Saya yg tiba2 senggugut hari majlis,Saya dn suami dtg lambat di lokasi outdoor.Agaknya mungkin sejam jugak mereka menunggu. *maafkan saye yee...* Tetapi hasil kerja yg mereka berikan tetap berkualiti. Ada umph laaa..:P..And if u like any pose tat u have seen before,atau tiba2 teringin nak bergambar ats pokok ke..just tell them..they will fulfill your wish..*err..boleh ke abg wan?..heheh* ...
The main causative agent of human urinary tract infections is the uropathogenic Escherichia coli (UPEC) pathotype. It may cause disease due to its ability to express a number of bacterial virulence factors. Fimbrial adhesins are particularly important for the initial establishment of infection in the urinary tract. The fimbriae are hair-like structures protruding from the bacterial cell and by attaching to specific receptors in the urinary tract they mediate adherence to different cell types, allowing the bacteria to resist the shear forces from urine flow. The UPEC strains generally carry multiple determinants for fimbrial adhesins. Previous studies have indicated that there is a co-regulation between different fimbrial genes and one factor that has been implicated in this is the PapB protein, acting as a transcriptional regulator of P-fimbrial expression. The PapB protein can be regarded as the prototype of a family of fimbrial regulators that show high homology between different fimbrial ...
Escherichia coli colonizes the human intestine shortly after birth, with most strains engaging in a commensal relationship. However, some E. coli strains have evolved toward acquiring genetic traits associated with virulence. Currently, five categories of enteroadherent E. coli strains are well-recognized, and are classified in regard to expressed adhesins and the strategy used during the colonization. The high morbidity associated with diarrhea has motivated investigations focusing on E. coli adhesins, as well on factors that inhibit bacterial adherence. Breastfeeding has proved to be the most effective strategy for preventing diarrhea in children. Aside from the immunoglobulin content, glycocompounds and oligosaccharides in breast milk play a critical role in the innate immunity against diarrheagenic E. coli strains. This review summarizes the colonization factors and virulence strategies exploited by diarrheagenic E. coli strains, addressing the inhibitory effects that oligosaccharides and
Urinary tract infections (UTIs) result in $1.6 billion in medical expenditures in the United States each year (1), with uropathogenic strains of Escherichia coli (UPEC) accounting for 70 to 95% of all UTIs (2). With the advance of multi-drug-resistant UPEC (3), it is important to determine the pathogenic mechanisms of UPEC. In animal models, UPEC pathogenesis initiates with bacterial binding of superficial bladder epithelial cells via the adhesin FimH at the tips of bacterially expressed type 1 pili (4). Initial colonization events activate inflammatory and apoptotic cascades in the epithelium, which is normally inert and only turns over every 6 to 12 months (5). Bladder epithelial cells respond to invading bacteria in part by recognizing bacterial lipopolysaccharide (LPS) via the Toll-like receptor 4 (TLR-4)-CD14 pathway, which results in strong neutrophil influx into the bladder (6). In addition, FimH-mediated interactions with the bladder epithelium stimulate exfoliation of superficial ...
UPEC utilizes complex mechanisms to subvert innate defenses to persist and cause disease. The ability of UPEC to invade into superficial cells of the bladder has been shown to be a critical mechanism in the ability of UPEC to establish a persistent infection (8, 12, 14, 15). Upon entry, UPEC diverts itself into the cytoplasm by an unknown mechanism that depends on the FimH adhesin present at the tips of type 1 pili (7). Previous light and electron microscopic studies identified loose intracellular collections of bacteria at early time points (≈6 h) in the superficial umbrella cells of the bladder, which were termed bacterial factories (8). In addition, at later time points (24 h) intracellular biofilm-like communities were observed that created a bulging appearance on the luminal surface of the bladder that were termed pods (15). Based on real-time fluorescence microscopy studies presented here, we discovered that these various structures are formed as part of a continuous developmental and ...
The paper in Nature Microbiology can be found here. The work presented in this paper was the result of a strong collaboration between the laboratories of Dr. Scott J. Hultgren and Dr. Peng Yuan at Washington University in St. Louis. Chaperone-usher pathway (CUP) pili are extracellular proteinaceous fibers ubiquitously found on Gram-negative bacteria. Type 1 and P pili are produced by uropathogenic strains of E. coli and are tipped with the FimH and PapG adhesins, respectively, to mediate host and tissue tropism to the bladder and kidney. During pilus assembly hundreds of individual pilus subunits called pilins are first exported across the inner membrane to the periplasm. Chaperone-pilin complexes are then guided to an outer membrane (OM) nanomachine called the usher, which catalyzes subunit-subunit interactions. The usher contains five functional domains: a 24-stranded transmembrane β-barrel domain, a β-sandwich plug domain that resides in the pore of the TD in the apo-usher, an ...
1O9Z: The Fimbrial Adhesin F17-G of Enterotoxigenic Escherichia Coli Has an Immunoglobulin-Like Lectin Domain that Binds N-Acetylglucosamine
1 Sequence alignment [w] Consider the strings APPLE and PAPE over the alphabet E = {A,E,L,P} and a penalty matrix P: A E L P A 1 E 1 1 L. 2 1 2 Compute the sequ
Vanwetswinkel, S., A. Volkov, Y. G. J. Sterckx, A. Garcia-Pino, L. Buts, W. F. Vranken, J. Bouckaert, R. Roy, L. Wyns, and N. A. J. van Nuland, Study of the structural and dynamic effects in the FimH adhesin upon α-d-heptyl mannose binding., J Med Chem, vol. 57, issue 4, pp. 1416-27, 2014 Feb 27. ...
Advocacy, Education, and Research Services The Asthma and Allergy Foundation of America (AAFA), a not-for-profit organization founded in 1953, is the leading organization for people with asthma...
Asthma and allergies impact one in five children in the United States. The Asthma and Allergy Foundation of America (AAFA) exhibited at the 2019 American Academy of Pediatrics (AAP) conference to learn the latest news in pediatrics and to talk to health care providers about the programs and services we offer.
Each year, the Asthma and Allergy Foundation of America (AAFA) declares May to be National Asthma and Allergy Awareness Month. Its a peak season for asthma and allergy sufferers, and a perfect time to educate your patients, family, friends, co-workers and others about these diseases.
Berichte zur Polar- und Meeresforschung (Reports on Polar and Marine Research), Bremerhaven, Alfred Wegener Institute for Polar and Marine Research, 473 , 103 p ...
Cara mengobati Gout - Gout merupakan bentuk dari artritis yang bisa mengakibatkan terjadinya rasa sakit secara tiba - tiba dan kemerahan, parah, sembap, penghangatan serta pembengkakan pada persendian. Penyakit ini…. ...
Cara mengobati Gout - Gout merupakan bentuk dari artritis yang bisa mengakibatkan terjadinya rasa sakit secara tiba - tiba dan kemerahan, parah, sembap, penghangatan serta pembengkakan pada persendian. Penyakit ini…. ...
saya students uitm arau, menyewa di taman sri wang fasa 3, lorong 28.. betul2 depan sawah.. saya merupakan mangsa terbaru gigitan cinta pakcik charlie nih,, iaitu malam semalam. mmg betul kate kamu sebab saye tido x tutup lampu. sedih kot, minggu depan nak raye dah.. kene kat muke pulak tuh. masalah pada saya, saye x kenal rupe pkcik charlie nih. kalo jumpe, ku gigit2 balik siap kamu!! ...
Great value bright Box files, high quality board with a paper cover. Flush fitting lid with a strong press-button catch. Interior lock-spring secures loose pape
Poslat na n j arm du t kood nc a kolonu pandur , chalupu zbourat a d dka zav t na 150 let. Je extr mn spole ensky nebezpe n !!! V echny papou ky na cel m sv t zlikvidovat a p b h napsat do u ebnic.. Ty vynalo en pen ze za to rozhodn stoj !!!. To je opravdu nesl chan .. Bo e a pape i, chra n s p ed t mito hrozn mi narkotiky, men ...
Saya baru je balik kerja ni. Now tgh tunggu mak, abah n adik2 sampai. Mereka bertolak tghhari td dr kedah. Skrg (6.00pm) dah ada di behrang. Menurut mak, ujan lebat sgt. Dianggarkan akan tiba lepas maghrib.. X kisahlah, asalkan semuanya selamat... ...
SUFU 2020 the range of Uropathogenic Escherichia Coli (UPEC) motility types UPEC strains from women with recurrent urinary tract infections (UTIs).
Many bacteria, both environmental and pathogenic, exhibit the property of autoaggregation. In autoaggregation (sometimes also called autoagglutination or flocculation), bacteria of the same type form multicellular clumps that eventually settle at the bottom of culture tubes. Autoaggregation is generally mediated by self-recognising surface structures, such as proteins and exopolysaccharides, which we term collectively as autoagglutinins. Although a widespread phenomenon, in most cases the function of autoaggregation is poorly understood, though there is evidence to show that aggregating bacteria are protected from environmental stresses or host responses. Autoaggregation is also often among the first steps in forming biofilms. Here, we review the current knowledge on autoaggregation, the role of autoaggregation in biofilm formation and pathogenesis, and molecular mechanisms leading to aggregation using specific examples ...
The chaperone-usher (CU) pathway is a translocation system used to assemble adhesive multi-subunit fibres on the outer surface of gram-negative bacteria. CU pili are formed by the non-covalent polymerisation of several hundreds or thousands of pilus subunits which consist of an incomplete immunoglobulin (Ig)-like fold lacking the C-terminal ß-strand. In the periplasm, a cognate chaperone assists in pilus subunit folding by donating a b-strand to complement the truncated Ig-like fold of the pilus subunit, a process termed donor-strand complementation (Figure 69, A*) [1]. Chaperone:subunit complexes are then recruited to a pilus assembly platform in the outer membrane (OM) called the "usher". The usher catalyses ordered subunit polymerisation and mediates translocation of the nascent pilus to the cell surface. Polymerisation of pilus subunits occurs through an intermolecular fold complementation mechanism involving the first 10-20 residues (termed "N-terminal extension" or Nte) of the pilus ...
Bacterial adhesins promote colonization at the initial stages of an infection by mediating attachment to host tissues, thus avoiding nonspecific host defenses such as mechanical clearance and allowing bacterial multiplication to occur within the host (1). To exert these functions, adhesins need to be presented at the surface of the bacterium. Like typical adhesins, B. pertussis FHA attaches the bacterium to receptors in the respiratory tract (17-21, 28). However, in addition to being surface-associated, large amounts of FHA are also released into the extracellular milieu (15). This has so far only been observed in vitro. In this work, we show for the first time that FHA is likely to also be released in vivo, and that its secretion is necessary for efficient colonization in a mouse model of infection. Our results support the paradigm that the secreted form of bacterial adhesins may participate in pathogenesis.. The use of B. pertussis strains deficient in FHA release but presenting FHA ...
WOODWARD, MJ and WRAY, C (1990) 9 DNA PROBES FOR DETECTION OF TOXIN AND ADHESIN GENES IN ESCHERICHIA-COLI ISOLATED FROM DIARRHEAL DISEASE IN ANIMALS ...
Tiba Tempeh is a natural plant-based protein made with organic soybeans. Sensational in stir-fries, salads, curries, wraps and more. Tasty, healthy, easy and sustainable... you can have it all when you grab life by the plants.
Shaffer, Carrie L. and Zhang, Ellisa W. and Dudley, Anne G. et al. (2017) Purine Biosynthesis Metabolically Constrains Intracellular Survival of Uropathogenic Escherichia coli. Infection and Immunity, 85 (1). Art. No. e00471-16. ISSN 0019-9567. PMCID PMC5203662. https://resolver.caltech.edu/CaltechAUTHORS:20170331-075358953 ...
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Aida - Name Meaning. Your name, Aida, gives you the desire to understand and help others ... Is the name of Aida helping you? Discover the full name meaning of Aida. Free Name Report.
Korang pernah tak mengalami bengkak pada mata yang terjadi dengan sendirinya? Entah dari mana tah dia datang. Tiba tiba je da muncul dekat kelopak mata. Pernah tak. Pernah tak? Orang biasa paggil ketumbit ke ketumbr tah. Aku pun tak sure. Ala, yang kalau orang nampak mata kita bengkak, orang akan selalu cakap: " Haa. Tula, gi ngitai orang mandi la tu." Ntah pape je kan alasan tuh. ...
Simple gila bukan heavy gila lil bit gila² tak suka makan gula² senyum manis macam air gula. Suka buat muka bikin entri ikut suka. Thanks reading ...
duh Top Thread ternyata, alhamdulillah :matabelo: kirain gak dibolehin sama perusahaan rokok itu :ngakaks terima kasih banyak yg udah rate n share.. semoga bermanfaat :) ===================================== Terngac.. eh Tercengang kan gan baca judulnya ? Apalagi ane gan sebagai perokok pasif yang tiba tiba disuruh menikmati manfaat dari asap rokok :( Oke deh langsung aja gan ane kasih