A generation ago, childhood vaccines werent so controversial. Doctors told parents that vaccines saved lives, and parents listened. But then, a widely publicized but since-retracted study in the Lancet journal linked vaccines with autism, and autism activists like Playboy model Jenny McCarthy ...
A generation ago, childhood vaccines werent so controversial. Doctors told parents that vaccines saved lives, and parents listened. But then, a widely publicized but since-retracted study in the Lancet journal linked vaccines with autism, and autism activists like Playboy model Jenny McCarthy ...
A new Pediatrics study found that nearly 90% of parents to young children vaccinate their children following the CDC recommended schedule. However, some parents still dont feel at ease...
Purpose: One of the key limiting parameters in the penetration and effectiveness of topically applied ocular therapeutics is their mean residence time. Most of an instilled solution clears through drainage, with first-order clearance about 4 times that of bulk tear flow (~5 ul/min). For protein drugs, the problem becomes more acute due to the additional burden of reduced penetrance through the cornea and conjunctiva. Residence time might be increased however by fusing a protein of interest to a mucosal surface binding protein, such as galectin-3 (gal-3). Gal-3 is a small pentameric ocular surface resident protein that cross-links O-type mucins.. Methods: We used Gaussia luciferase (luc) as our test protein, fused at its N-terminus to one or 2 copies of the carbohydrate binding domain of gal-3 (gal). Fusions were expressed in HEK cells and purified by IMAC chromatography, and evaluated mucin binding by ELISA and by their ocular surface half-lives on mouse eyes and rabbit corneas, using luciferase ...
Do you have questions about vaccinations for your children? Dr. Randall Neustaedter addresses choosing vaccines, the adverse side-effects, legal requirements and exemptions, as well as alternative vaccines.
We have received an update from Public Health England with regards to hepatitis A vaccine supply. There is an ongoing global shortage which is impacting on continuity of supply in the UK. Anybody requiring boosters of this vaccination can be safely delayed at the present time. We are currently investigating alternative vaccine options as advised by Public Health England. ...
The monumental 2017 publication, "Arts of Living on a Damaged Planet," invites readers to learn new ways to understand ourselves in the face of unprecedented social and ecological turmoil.. As part of this rethinking, scholars marshal research that upends the most fundamental unit of biological and social analysis - the individual. Building from the work of famed University of Massachusetts Amherst Professor Lynn Margulis, one essay shows the proliferation and importance of nonhuman cells that comprise human bodies. Another essay proposes that evolution depends in part on relationships between species, and not simply individual fitness. And yet another essay argues that collective behavior might best be understood as an emergent process, rather than as a planned outcome.. These growing scientific insights are profound. They suggest that our very existence depends not on individual success, but on a deepening understanding of and attunement to our interrelationships with others - both human and ...
At ABB, we understand the challenges you face in the food and beverage processing industry today. Were focused on providing electrical solutions that address the critical issues in every area of your operation, so you can focus on plant sustainability, cost, quality, flexibility, safety and regulatory challenges across the production cycle. Our family of electrical solutions matches specific application criteria from start to finish inside food processing areas, assuring the quality and reliability of your electrical system throughout your facility, from incoming raw materials through shipping of finished goods. And with the industrys most efficient distribution system, were prepared to meet your ongoing MRO, OEM and construction needs down the road.. ...
We evaluated the prevalence of hepatitis E virus (HEV) in the pork production chain in Czech Republic, Italy, and Spain during 2010. A total of 337 fecal, liver, and meat samples from animals at slaughterhouses were tested for HEV by real-time quantitative PCR. Overall, HEV was higher in Italy (53%) and Spain (39%) than in Czech Republic (7.5%). HEV was detected most frequently in feces in Italy (41%) and Spain (39%) and in liver (5%) and meat (2.5%) in Czech Republic. Of 313 sausages sampled at processing and point of sale, HEV was detected only in Spain (6%). HEV sequencing confirmed only g3 HEV strains. Indicator virus (porcine adenovirus) was ubiquitous in fecal samples and absent in liver samples and was detected in 1 slaughterhouse meat sample. At point of sale, we found porcine adenovirus in sausages (1%-2%). The possible dissemination of HEV and other fecal viruses through pork production demands containment measures ...
Dear AAP Pediatricians, As a physician, scientist, and father of a vaccine-injured child, I have many issues with Offit and Moser�s critique of Dr. Sears vaccine book, particularly its authoritarian tone and content. Offit is certainly entitled to his opinion, but it must be recognized as that. We must stick to the science and recognize the open questions with regards to vaccine safety. Excerpt from Offit and Moser article: {Sears has a poor grasp of the scientific method. "Some studies have been published in recent years that have failed to show statistical proof of a relationship between vaccines and autism," he writes. "However, by the same token, it is also difficult to prove that there is not a connection." Using the scientific method, investigators form the null hypothesis. Good epidemiological studies are powered to reject or not to reject the null hypothesis. However, the scientific method does not allow investigators to accept the null hypothesis. Said another way, scientists can ...
Cells derived from different organisms and tissues have been established and are used in bioresearch and for the production of complex biological products. The cell lines can be grown in labs for extended periods, but changes in the DNA sequence and biological responses of the cells can occur. The changes in the cell lines during culturing or inadvertent mixing or mislabeling cannot be readily identified by their appearance under a microscope. A major issue has continued to plague the scientific community for over 50 years: cell line contamination and misidentification. Human cell lines can be identified by existing multiplex polymerase chain reaction (PCR) assays that target short tandem repeat (STR) markers in DNA that are used as fingerprints for authentication. Methods to authenticate nonhuman cell lines are lacking. Our efforts have focused on developing methods for authentication of important nonhuman cell lines to achieve measurement assurance of their use.
M2.DM.17.4769) A five-year-old boy presents with his mother to your clinic complaining of a fever and rash. His mother says the patient was in his usual state of health until two weeks ago when he developed "strep throat." He was prescribed a ten day treatment with oral amoxicillin by his primary care physician, which he has since completed. The mother reports that he seemed to feel better until two days ago when he began to complain of joint pain and an itchy rash. His mother notes the patient is on an "alternative vaccine schedule." She is nervous because her son attends day-care three days a week, and there has been a recent "viral" outbreak. The patients temperature is 103°F (39.4°C), blood pressure is 99/59 mmHg, pulse is 110/min, and respirations are 20/min with an oxygen saturation of 99% O2 on room air. On physical exam, you note cervical adenopathy, joint swelling, mild peripheral edema, and a diffuse rash (shown in Figure A). Which of the following is the best treatment for this ...
Flaviviruses were proposed to prevent rather than to induce host translation shutoff (36, 37, 40). Several technical aspects might explain this difference from our findings, including the use of nonhuman cells, different DENV serotype 2 strains, and time points p.i. chosen for analysis. Of note, polysome profiles of Huh7 cells infected with the DENV serotype 2 Bangkok strain 16681 (40) revealed in our experiments a similar translation repression to that induced by DENV1 infection (see Fig. 1F for reference), which also correlated with reduced cytopathic effect in Huh7 cells (data not shown). Analyses of human cells early p.i. by polysome profiling identified host cell translation repression as a new feature of flavivirus infection.. Flaviviruses actively block eIF2α-mediated stress response at different levels, supposedly to avoid the associated host translation suppression. First, WNV and DENV inhibit SG formation in nonhuman cells (36). Consistently, we observed that DENV and WNV, as well as ...
Since were on the note of breastfeeding, let me tell you new mommies how important chlorella is for you specifically. Breastfeeding provides your baby with a huge extra load of nutrients and boosts his/her immunity. The exact composition of breast milk changes with each feeding to adapt to your individual babys needs. A study in Japan followed 35 breast-feeding mothers. Eighteen of them took chlorella supplements and were compared to the rest. Breast milk from mothers who supplemented with chlorella had higher concentrations of Immunoglobulin A (IgA), which is an antibody. These antibodies help prevent viruses, bacteria and fungus from creating infections (very important if you are doing an alternative vaccine schedule, or choosing not to vaccinate at this time). IgA, in particular, helps protect the surfaces of the body that are exposed to foreign substances like the nose, digestive tract, eyes, ears, saliva and tears. This is a big advantage to young babies. This same Japanese study showed ...
As acknowledged, biomass has been identified as the most promising alternative for replacing fossil fuel due to its sustainability. Grinding is known...
Infectious bronchitis virus (IBV) causes an acute, highly contagious respiratory disease in chickens, which results in significant economic losses in commercial flocks. One of the major hurdles associated with the control of the disease is the continued emergence of new serotypes/variants due to mutation or recombination. This also makes the diagnosis of disease using conventional serological methods difficult. Therefore, there is a need for alternative vaccines and serotyping methods. ^ A multiplex PCR for Massachusetts and Arkansas serotypes of IBV was developed. Two serotype specific PCR products, 1026 bp for Massachusetts and 896 bp for Arkansas were amplified; the detection limits for both were 5 pg of viral RNA. By testing different isolates/serotypes of IBV and other avian bacterial and viral pathogens, the high specificity of the multiplex PCR was demonstrated. In addition, its ability to detect Massachusetts and Arkansas as co-infections was revealed. ^ To explore the potential use of
The vaccine. There are two possible vaccines for Japanese encephalitis. Ideally, they need to be completed a month before you leave. One vaccine requires two doses, with the second dose given 28 days after the first. This vaccine is only suitable for people who are over 18 years of age. The alternative vaccine consists of three doses, and is suitable for people who are over one year of age. The second dose is given after seven days, and the third dose is given 21 days after this. This vaccine needs to be completed at least 10 days before you leave, in case you have an allergic reaction. Both vaccines will require a booster after one year. The Japanese encephalitis vaccine is not suitable for children who are under one year of age.. If there is not enough time to complete a normal course of the vaccine, you may be able to have an accelerated course. This involves two doses being given one week apart, or three doses with a week in between each dose. This still needs to be completed at least 10 ...
Pneumococcal surface protein A (PspA) and pneumococcal surface protein C (PspC) are important candidates for an alternative vaccine against pneumococcal infections. Since these antigens show variability, the use of variants that do not afford broad protection may lead to the selection of vaccine escape bacteria. Epitopes capable of inducing antibodies with broad cross-reactivities should thus be the preferred antigens. In this work, experiments using peptide arrays show that most linear epitopes recognized by antibodies induced in mice against different PspAs were located at the initial 44 amino acids of the mature protein and that antibodies against these linear epitopes did not confer protection against a lethal challenge. Conversely, linear epitopes recognized by antibodies to PspC included the consensus sequences involved in the interaction with human factor H and secretory immunoglobulin A (sIgA). Since linear epitopes of PspA were not protective, larger overlapping fragments containing 100 ...
A new blood thinner might be a viable alternative to warfarin (Coumadin), the standard for decades to treat patients with the dangerous heart rhythm disorder kn
Mycobacterium tuberculosis (MTB), which is the causative agent of tuberculosis (TB), is among the most important infectious bacteria with high morbidity and mortality rates worldwide. Bacilli Calmette-Guerin (BCG) vaccine has been discovered for about a century, and it is considered as a major vaccine for humans. However, some factors, such as its attenuated nature and its inefficacy against the latent form of the disease, have led to the use of alternative vaccines. Multi-epitope subunit vaccines are new-generation vaccines that are being developed in clinical trial phases. For the production of a subunit vaccine, the selection of immunodominant antigens and targeted delivery systems to antigen presenting cells (APCs) are considered as basic parameters. In the present study, we designed the novel multi-epitope ESAT-6:Ag85B:Fcγ2a, which was evaluated completely by various online tools as an optimum vaccine against TB. The early secreted antigenic target of 6 kDa (ESAT-6) and antigen 85B (Ag85B)
The BCG vaccine has been widely available for several decades. It is easy and cheap to produce, and when given to neonates or young children it is effective in preventing severe manifestations of disease such as meningeal tuberculosis and miliary tuberculosis. However, in terms of the capacity of the vaccine to protect adult humans it shows a wide range of efficacy, including zero levels of protection. Due to the general realization that BCG is losing its protective effect, particularly in terms of preventing adult-onset tuberculosis, a major effort has been made to try to develop new alternative vaccines. One such candidate, Mtb72F/AS02A, is a polyprotein derived from two known M. tuberculosis antigens adjuvanted with AS02A. Mtb72F/AS02A is a candidate TB vaccine under development for two indications: prevention of primary TB infection in young children in highly endemic areas and as an adjunct to treatment for TB in adolescents and adults ...
Vertical transmission from an infected cow to its fetus accounts for the vast majority of new Neospora caninum infections in cattle. A vaccine composed of a chimeric antigen named recNcMIC3-1-R, based on predicted immunogenic domains of the two microneme proteins NcMIC1 and NcMIC3, the rhoptry protein NcROP2, and emulsified in saponin adjuvants, significantly reduced the cerebral infection in non-pregnant BALB/c mice. Protection was associated with a mixed Th1/Th2-type cytokine response. However, the same vaccine formulation elicited a Th2-type immune response in pregnant mice and did not prevent vertical transmission or disease, neither in dams nor in offspring mice. In this study, an alternative vaccine formulation containing recNcMIC3-1-R emulsified in Freunds incomplete adjuvant, a stimulator of the cellular immunity, was investigated. No protection against vertical transmission and cerebral infection in the pregnant mice and a very limited protective effect in the non-pregnant mice were observed.
Vertical transmission from an infected cow to its fetus accounts for the vast majority of new Neospora caninum infections in cattle. A vaccine composed of a chimeric antigen named recNcMIC3-1-R, based on predicted immunogenic domains of the two microneme proteins NcMIC1 and NcMIC3, the rhoptry protein NcROP2, and emulsified in saponin adjuvants, significantly reduced the cerebral infection in non-pregnant BALB/c mice. Protection was associated with a mixed Th1/Th2-type cytokine response. However, the same vaccine formulation elicited a Th2-type immune response in pregnant mice and did not prevent vertical transmission or disease, neither in dams nor in offspring mice. In this study, an alternative vaccine formulation containing recNcMIC3-1-R emulsified in Freunds incomplete adjuvant, a stimulator of the cellular immunity, was investigated. No protection against vertical transmission and cerebral infection in the pregnant mice and a very limited protective effect in the non-pregnant mice were observed.
Human immunodeficiency virus-type 1 (HIV-1) entry requires fusion cofactors on the CD4+ target cell. Fusin, a heterotrimeric GTP-binding protein (G protein)-coupled receptor, serves as a cofactor for T cell line-tropic isolates. The chemokines RANTES, MIP-1α, and MIP-1β, which suppress infection by macrophage-tropic isolates, selectively inhibited cell fusion mediated by the corresponding envelope glycoproteins (Envs). Recombinant CC CKR5, a G protein-coupled receptor for these chemokines, rendered CD4-expressing nonhuman cells fusion-competent preferentially with macrophage-tropic Envs. CC CKR5 messenger RNA was detected selectively in cell types susceptible to macrophage-tropic isolates. CC CKR5 is thus a fusion cofactor for macrophage-tropic HIV-1 strains. ...
0691133255 A Shoemakers Story: Being Chiefly about French Canadian Immigrants, Enterprising Photographers, Rascal Yankees, and Chinese Cobblers in a Nineteenth-Century Factory Town,books, textbooks, text book
Hydrogen offers a promising alternative for supplying clean and sustainable energy to meet increasing demands worldwide. However, materials are key to transforming the technology into a viable industry. Materials for the Hydrogen Economy describes the…
Tidal stream energy has emerged as one of the most promising alternative energy sources to the use of fossil fuels. Nevertheless, there are some crucial is