The effect of long-chain acyl-CoA on subcellular adenine nucleotide systems was studied in the intact liver cell. Long-chain acyl-CoA content was varied by varying the nutritional state (fed and starved states) or by addition of oleate. Starvation led to an increase in the mitochondrial and a decrease in the cytosolic ATP/ADP ratio in liver both in vivo and in the isolated perfused organ as compared with the fed state. The changes were reversed on re-feeding glucose in liver in vivo or on infusion of substrates (glucose, glycerol) in the perfused liver, respectively. Similar changes in mitochondrial and cytosolic ATP/ADP ratios occurred on addition of oleate, but, importantly, not with a short-chain fatty acid such as octanoate. It is concluded that long-chain acyl-CoA exerts an inhibitory effect on mitochondrial adenine nucleotide translocation in the intact cell, as was previously postulated in the literature from data obtained with isolated mitochondria. The physiological relevance with ...
1NY3: Catalytically active MAP KAP kinase 2 structures in complex with staurosporine and ADP reveal differences with the autoinhibited enzyme
Structures of ATP- and ADP-bound NSFa, Side-view of ATP-bound NSF. b, Side-view ofADP-bound NSF. The six protomer chains are rainbow colored counterclockwise ba
个人简历. 2004年获博士学位,现为遗传与细胞研究所教授、博士生导师。2008-2010年在美国德克萨斯大学医学分校进行博士后研究。2012-2013年在美国德克萨斯大学医学分校进行国际合作研究。多年来从事炎症的信号转导及基因表达调控机制研究。包括两个主要研究方向:1)DNA氧化损伤修复过程偶联基因转录及天然免疫病理发展的分子机制;2)蛋白质的多聚ADP核糖化(PAR化)对基因表达调控的影响、参与炎性疾病病理发展的机制。共发表SCI论文40余篇。主持在研自然科学基金面上项目课题1项,吉林省科技厅自然科学基金1项,及国际合作基金1项。主持完成自然科学基金面上项目课题1项,参加完成自然科学基金面上项目课题1项,参加国家重点基础研究发展规划项目(973)项目2项。学习经历: 1986年--1990年:东北师范大学 生命科学学院 生物学专业 学士 ...
The TReatment with ADP receptor iNhibitorS: Longitudinal Assessment of Treatment Patterns and Events after Acute Coronary Syndrome (TRANSLATE-ACS) study is a prospective, observational longitudinal study to evaluate the real world effectiveness and use of prasugrel and other ADP receptor inhibitor therapies among myocardial infarction (MI) participants treated with percutaneous coronary intervention (PCI) during the index hospitalization. Participant management and treatment decisions are at the discretion of the care team per routine clinical practice. Approximately 17,000 participants will be enrolled at approximately 350 sites in the United States. Follow-up will be conducted through 15 months in approximately 15,650 participants.. TRANSLATE-ACS will complement the results of both randomized controlled clinical trials and current registries in addressing the real world treatment patterns and clinical outcomes for MI participants managed with PCI and initiated on ADP receptor inhibitor ...
Adenosine Diphosphate: Adenosine 5-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5-position.
Adenosine diphosphate (or ADP) is the chemical that plants make ATP from, during photosynthesis. A chemical compound that can be converted to ATP with the addition of one phosphate group. ...
The only disclosure restriction on the PI is that the sponsor can review results communications prior to public release and can embargo communications regarding trial results for a period that is more than 60 days but less than or equal to 180 days. The sponsor cannot require changes to the communication and cannot extend the embargo ...
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Synonyms for ADP in Free Thesaurus. Antonyms for ADP. 2 synonyms for ADP: adenosine diphosphate, automatic data processing. What are synonyms for ADP?
The second mechanism of respiratory control (allosteric ATP-inhibition of cytochrome c oxidase (CcO)) is demonstrated for the first time in intact isolated rat liver and heart mitochondria. The problems of measuring the kinetics of allosteric ATP-inhibition in isolated mitochondria were investigated. And it was found that only at very high ATP/ADP ratios, this inhibition is obtained and requires an ATP-regenerating system consisting of phosphoenolpyruvate (PEP) and pyruvate kinase (PK). The allosteric ATP-inhibition can be switched off probably by dephosphorylation of a serine at CcO subunit-I. The phosphorylation of CcO at serine, threonine and tyrosine was studied in isolated mitochondria by extracting complex IV of the respiratory chain (CcO) by BN-PAGE (blue-nativepolyacrylamide- gel-electrophoresis), SDS-PAGE and Western blotting with the corresponding antibodies against the phosphorylated amino acids. The extent of allosteric ATP-inhibition of CcO varied in different preparations of ...
View Notes - W3OutlineLec7 from BIOL D103 at UC Irvine. • Thymosin-Beta4 Sequesters ATP-actin Have plenty of ATP-actin BUTbut it would be
|Mechanism for increasing muscle endurance via the small molecules AICARAICAR is a nucleoside that is taken up into muscle and converted into the nucleotide ZMP, which mimics the effects of the natural ligand, 5-AMP, on AMPK. The latter is produced by adenylate kinase acting on ADP generated from ATP during muscle contraction. Activated AMPK in…
Interested in using Martini for ADP or ATP with actin?. The attached .zip -file contains topologies for ADP, ATP as well as ADP-actin and ATP-actin systems. PDB files are provided for ADP- and ATP-actin, as well as the F-actin strand. As an added bonus, theres a short bash script which allows the user to build fibers of arbitrary lengths from one of the PDB files (2zwh.pdb).. Download the files here!. ...
LG Get product support for the LG T1603ADP5. Download T1603ADP5 manuals, documents, and software. View T1603ADP5 warranty information and schedule services.
At ADP, we believe that differences are what make us a stronger, smarter, more successful organization, and we believe the same to be true in our communities.
多种适用的ATP5LELISA试剂盒,如小鸡, Cow, 犬等。在antibodies-online.cn对比ATP5LELISA试剂盒,以便找到您需要的产品。
鈉鉀泵可以將細胞外相對細胞内較低濃度的鉀離子送進細胞,並將細胞内相對細胞外較低濃度的鈉離子送出細胞。經由以具放射性的鈉、鉀離子標定,可以發現鈉、鉀離子都會經過這個通道,鈉、鉀離子的濃度在細胞膜兩側也都是相互依賴的,所以顯示了鈉、鉀離子都可以經過這個載體運輸。目前已知鈉鉀泵需消耗ATP,並可以將三個鈉離子送出細胞,同時將兩個鉀離子送進細胞。 鈉鉀泵在1950年被丹麥的科學家延斯·斯科(Jens Skou)發現,它代表了我們對離子進出細胞的認識的一個重要的里程碑。它也在細胞刺激上有著重要的意義,像神經細胞的衝動,就是用鈉鉀泵幫助維持細胞電位使神經衝動得以傳輸。 ...
鈉鉀泵可以將細胞外相對細胞内較低濃度的鉀離子送進細胞,並將細胞内相對細胞外較低濃度的鈉離子送出細胞。經由以具放射性的鈉、鉀離子標定,可以發現鈉、鉀離子都會經過這個通道,鈉、鉀離子的濃度在細胞膜兩側也都是相互依賴的,所以顯示了鈉、鉀離子都可以經過這個載體運輸。目前已知鈉鉀泵需消耗ATP,並可以將三個鈉離子送出細胞,同時將兩個鉀離子送進細胞。 鈉鉀泵在1950年被丹麥的科學家延斯·斯科(Jens Skou)發現,它代表了我們對離子進出細胞的認識的一個重要的里程碑。它也在細胞刺激上有著重要的意義,像神經細胞的衝動,就是用鈉鉀泵幫助維持細胞電位使神經衝動得以傳輸。 ...
BioAssay record AID 336312 submitted by ChEMBL: Antiplatelet activity against bovine citreated platelet assessed as inhibition of ADP-induced platelet aggregation up to 278 ug/ml after 6 mins.
The effect of MgADP on the sarcomere length (SL) dependence of tension generation was investigated using skinned rat ventricular trabeculae. Increasing SL from 1.9 to 2.3 microm decreased the muscle width by approximately 11% and shifted the midpoint of the pCa-tension relationship (pCa(50)) leftward by about 0.2 pCa units. MgADP (0.1, 1, and 5 mmol/L) augmented maximal and submaximal Ca(2+)-activated tension and concomitantly diminished the SL-dependent shift of pCa(50) in a concentration-dependent manner. In contrast, pimobendan, a Ca(2+) sensitizer, which promotes Ca(2+) binding to troponin C (TnC), exhibited no effect on the SL-dependent shift of pCa(50), suggesting that TnC does not participate in the modulation of SL-dependent tension generation by MgADP. At a SL of 1. 9 microm, osmotic compression, produced by 5% wt/vol dextran (molecular weight approximately 464 000), reduced the muscle width by approximately 13% and shifted pCa(50) leftward to a similar degree as that observed when increasing
PAIVA NOVAES, Myriam Stella de; FERREIRA, F. D; NICOLAU, J. Adenine nucleotide contents and atpases activities in porcine deciduous dental pulp during the root formation, fully formed root and root resorption phases. Journal de Biologie Buccale, Paris, v. 17, p. 187-92, 1989 ...
13 sentence examples: 1. Adenosine diphosphate is one of the most important mediators of both physiological hemostasis and thrombosis. 2. Method Platelet aggregation was induced by adenosine diphosphate ( ADP ) in rabbits. 3. ADP ( adenosine diphosph
To compare the newer inductor of platelet aggregation cationic propyl gallate (CPG) with adenosine diphosphate (ADP) for the examination of aspirin (ASA) effectivity with optical aggregometry. In total,116 patients were prospectively enrolled with a stable cardiovascular disease, taking ASA 100 mg/day for |or=1 month. The control group consisted of 62 healthy volunteers. A platelet aggregation was investigated by optical aggregometry (aggregometer LASER 4x; BIO ART, Sint-Katelijne-Waver, Belgium). CPG and ADP were added as aggregating agents. The measured parameters were CPG-slope (%/min) and ADP max (%). Using the CPG-slope values from the control group, the CPG-slope cut-off value was determined to define a laboratory ASA-noneffectively treated patient. The values from control group followed a normal distribution (Shapiro-Wilk test). We calculated the cut-off value using the 1-tailed 95% confidence interval. The CPG-slope cut-off value was 79 %/min for an ASA-effectively treated patient. We marked the
The human body is "powered", essentially, by a substance called Adenosine Triphosphate (ATP). This substance is regarded as the "energy unit" of life. To use this energy source to, for example, contract a muscle, ATP is converted to Adenosine Diphosphate (ADP), resulting in a release of energy. Over time, ADP can be converted back into ADT using the energy obtained from food. ATP can be regarded as the "charged" state, and ADP can be regarded as the "discharged" state of a battery.. This process could be used in two ways.. Firstly, it may be possible to create an "Organic Battery" which converts chemical energy from this process into electrical energy. I found THIS link which seems to indicate that some work is already being done on this process. The only questions would be regarding how small such a power source could be, how efficient the energy transfer would be, and how the battery could be recharged in real-world conditions. This could potentially solve a difficult engineering problem, ...
Intravenous and oral administration of a chemically stable carboprostacyclin analogue, 15-cyclopentyl-ω-pentanor-5(E)-carbacyclin (ONO 41483), resulted in ex-vivo inhibition of ADP-induced platelet aggregation in man. The maximum tolerated intravenous dose was 2.5 ng/kg/min for 1 hour and this produced a mean of 27.1% inhibition in 3 volunteers. For oral administration the tolerated single dose was 200 μg. At this dose, there was 56.3% inhibition of aggregation (mean of 3 results). High oral (400 μg) and intravenous doses (5 and 10 ng/kg/min for 1 hour) of ONO 41483, which caused marked inhibition of aggregation (ranging 39-100%), was accompanied by flushing of face and extremities, headache and phlebitis. However, none of the doses tested produced significant changes in arterial blood pressure or heart rate ...
Approach and Results-Our goal is to determine whether a novel antibody targeting the ligand-binding domain, ie, second extracellular loop (EL2) of the P2Y1R (EL2Ab) could inhibit platelet function and protect against thrombogenesis. Our results revealed that the EL2Ab does indeed inhibit ADP-induced platelet aggregation, in a dose-dependent manner. Furthermore, EL2Ab was found to inhibit integrin GPIIb-IIIa activation, dense and α granule secretion, and phosphatidylserine exposure. These inhibitory effects translated into protection against thrombus formation, as evident by a prolonged time for occlusion in a FeCl3-induced thrombosis model, but this was accompanied by a prolonged tail bleeding time. We also observed a dose-dependent displacement of the radiolabeled P2Y1R antagonist [3H]MRS2500 from its ligand-binding site by EL2Ab. ...
Summary: In Dugesia tigrina, complex I and the ADP phosphorylation system are involved in the loss of oxidative phosphorylation capacity when temperature decreases. These steps are able to adjust following cold acclimation, allowing the animals to occupy a wide thermal range. ...
PDRP is an unusual bifunctional ADP-dependent phosphotransferase involved in the regulation of C4 plant photosynthesis. Despite thirty years of research since its discovery, biophysical, kinetic, and structural characterisations have remained elusive. The discovery of a homologous protein (YdiA) in E. coli provides an unparalleled opportunity to obtain such characterisations. For the first time, YdiA has been cloned, overexpressed, and the biophysical characteristics have been determined. The protein was found to be dimeric by gel filtration, native-PAGE, and AUC. ITC was used to characterise binding affinity for the putative substrates. The protein binds in a largely temperature and pH-independent manner to ADP, AMP, phosphate, and pyrophosphate, in the uM range, with KdS of \.24 ± 0. 76, 5.23 ± l.l0, 15.54 ± 4.30, and 24.18 ± 2.70 respectively. The putative macromolecular substrate, PPS, was cloned, overexpressed, and characterised. This substrate was found to be dimeric at high ...
BioAssay record AID 54289 submitted by ChEMBL: Compound was tested at 1 nM for the inhibition of ADP-induced (20 uM) platelet aggregation in bovine seminal vesicle microsomes; NI=No inhibition.
The primary role of creatine is recharging ATP, by donating a phosphate ion to the ADP molecule. Muscle fibres generate tension by cutting off a phosphate group from ATP (charged form), turning it into ADP (uncharged from).. Three energy systems provide energy to recharge ADP back into ATP: the ATP-phosphocreatine system, the lactic acid system and the aerobic system. The percentage each energy system contributes depends on the intensity and duration of the activity.. The ATP-phosphocreatine system is the major energy provider for high-intense, explosive actions. This system is limited however by the amount of phosphocreatine that is stored in the muscles. After approximately 10 seconds of high-intense activity all the phosphocreatine is depleted and the ATP-phosphocreatine system can no longer contribute to energy production (recharging ADP into ATP) until it is replenished (approximately 3 minutes of rest). The power output drops when phosphocreatine levels in the muscle cells gets depleted, ...
A Calcium-activated enzyme that catalyzes the Hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and Diphosphates. EC 3.6.1.5 ...
Chapter 13 - ATP Hydrolysis as a source of energy The molecular details of ATP hydrolysis - ΔG when ATP is split into ADP and P - Motors move along a ΔG gradient - Motors move faster with higher [ATP] - Structure of ATP and the hydrolysis reaction - At least 8 distinct states are involved in motor stepping - Case study: the complete ATP hydrolysis cycle of Myosin II ...
Energy for this comes from ATP, the energy source of the cell. ATP binds to the cross bridges between myosin heads and actin filaments. The release of energy powers the swiveling of the myosin head. Muscles store little ATP and so must continuously recycle the discharged adenosine diphosphate molecule (ADP) into ATP rapidly. Muscle tissue also contains a stored supply of a fast acting recharge chemical, creatine phosphate which can assist initially producing the rapid regeneration of ADP into ATP ...
The difficulties encountered in the crystallization of membrane proteins in a form suitable for X-ray analysis have stimulated the development of algorithms to predict, from primary amino acid...
Immobilized Nucleotides for Affinity Chromatography Affinity Chromatography Kits for Adenosine Nucleotide binding Proteins Affinity Kit Components
A method for forming a MEMS device is disclosed, where a final release step is performed just prior to a wafer bonding step to protect the MEMS device from contamination, physical contact, or other deleterious external events. Without additional changes to the MEMS structure between release and wafer bonding and singulation, except for an optional stiction treatment, the MEMS device is best protected and overall process flow is improved. The method is applicable to the production of any MEMS device and is particularly beneficial in the making of fragile micromirrors.
PA3467U-1ACA , PA3396U-1ACA,PA3165U-1ACA / PA3380E-1ACA / PA3380U-1ACA / PA3396E-1ACA / PA3432E-1AC3 / PA3432E-1ACA / PA3432U-1AC3 / PA3432U-1ACA / PA3467E / PA3467E-1ACA / PA3467U-1ACA / PA3468E / PA3468E-1ACA / PA3468U / PA3468U-1ACA/ PA3467 / PA3467U / SADP-65KB PA-1500-02 / PA-1600-01 / PA-1600-02 / PA-1650-1601 PA-1650-01 / PA-1650-02 / PA-1700-02 / PA-1750-01 / PA-1750 -04 / PA-1900-15 / 6500767/6500920 / 0335C1965 / 0220A1990 P / N 2521997/5534 / ADP45CB / 10244/102458/102918/103310/103739/103905/103907/105926/105927/105928 / ACE83-110087-3100 / ACE83-110114-7000 / ACE83-110114-7100 / ACE83-110128-0200. A000001200 / A000001210 / A000007020 / A000007030 / ACC10 / ADP-65DB / ADP-65HB / ADP-75FB-A / ADT-W61 / API1AD43 / K000000550 / K000004120 / K000005050 / K000019570 / K000025320 / K000027270 / K000029300 / K000032420 / K000032580 / K000040250 / K000040270 / K000040290 / K000040460 / K000041670 / K000042840 / K000043680 / SADP - 65KB / SADP-65KBB / V000055210 / V000055400 / V000061300 / ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
1H8E: Structure of Bovine Mitochondrial F1-ATPase with Nucleotide Bound to All Three Catalytic Sites: Implications for the Mechanism of Rotary Catalysis
You are viewing an interactive 3D depiction of the molecule n-benzyladenosine 5-(trihydrogen diphosphate) (C17H21N5O10P2) from the PQR.
Effect of Qter® treatment on ATP, protein content and cell growth in H9c2 cells.H9c2 cells were treated up to 72 hours with 100 nM Qter® and the ATP content w
The thienopyridine anti-platelet drugs, such as clopidogrel, require metabolic activation in vivo to effectively block platelet aggregation. The study of the activation of these compounds has been hampered by the lability and reactivity of the ring-opened active metabolite. Many studies have relied solely on the disappearance of the parent drug as an indicator of bioactivation by a particular cytochrome P450 (CYP). We have developed an alternative method for studying the formation of the active metabolite. Conditions were optimized whereby washed human platelets can be incubated in the presence of an individual, recombinant CYP and clopidogrel. At various time points during this incubation, an aliquot is removed and platelet aggregation is measured using 2-(Methylthio)adenosine 5′-diphosphate (2MeSADP) or adenosine 5′-diphosphate (ADP) as the agonist. Inhibition of platelet aggregation, compared to the control lacking active enzyme, suggests the formation of the thienopyridine active ...
An uncoupling protein (UCP) is a mitochondrial inner membrane protein that is a regulated proton channel or transporter. An uncoupling protein is thus capable of dissipating the proton gradient generated by NADH-powered pumping of protons from the mitochondrial matrix to the mitochondrial intermembrane space. The energy lost in dissipating the proton gradient via UCPs is not used to do biochemical work. Instead, heat is generated. This is what links UCP to thermogenesis. UCPs are positioned in the same membrane as the ATP synthase, which is also a proton channel. The two proteins thus work in parallel with one generating heat and the other generating ATP from ADP and inorganic phosphate, the last step in oxidative phosphorylation. Mitochondria respiration is coupled to ATP synthesis (ADP phosphorylation) but is regulated by UCPs. There are five types of homologs known in mammals: UCP1, also known as thermogenin UCP2 UCP3 SLC25A27, also known as "UCP4" SLC25A14, also known as "UCP5" Uncoupling ...
Ticagrelor (AZD6140) is the first reversibly binding oral P2Y(12) receptor antagonist that blocks ADP-induced platelet aggregation.
LABORATORY ADHERENCE TO ISTH GUIDELINES ON PLATELET AGGREGATION TESTING. INITIAL REVIEW OF RESULTS FROM AN INTERNATIONAL SURVEY. Conference Paper ...
The synthesis of [2-3H]ATP with specific activity high enough to use for 3H NMR spectroscopy at micromolar concentrations was accomplished by tritiodehalog
Low-dose, long-term aspirin use irreversibly blocks the formation of thromboxane A2 in platelets, producing an inhibitory effect on platelet aggregation.. ...
Spinal cord damage (SCI) frequently results in everlasting disability or decline of movement (paralyE-7438sis) and feeling below the website of damage leading
The kit is intended for determination of intracellular ATP (adenosine triphosphate) in living cells. Extracellular ATP in the samples is enzymatically degraded. If the samples also contain somatic cells and the intention is to determine microbial ATP, we recommend 266-112 Microbial ATP Kit including a Cell Lysing Reagent for most animal cells. 266-311 ATP Biomass Kit can be used, if there is only one type of cells and no extracellular ATP in the samples. All living cells contain ATP where it plays the role of energy currency between different cellular processes. When cells die of natural causes, ATP is normally degraded. The intracellular concentration of ATP is carefully regulated to similar levels in all types of cells. ATP is therefore a good estimate of the total intracellular volume. Most bacterial cells contain approx. 2×10 -18 mol ATP per cell, while most eukaryotic cells, as a result of their larger size, contain 10 -15 mol ATP or more. ATP Biomass Kit HS, Intracellular ATP Kit HS and ...