Phylogenetic analysis of Streptomyces tenebrarius NBRC 16177 on the basis of the 16S rRNA gene sequence revealed that this organism was related to Streptoalloteichus hindustanus. It possessed glutamic acid, alanine and meso-diaminopimelic acid as cell-wall amino acids, galactose, mannose and glucose as whole-cell sugars and the menaquinones MK-10(H6), MK-10(H4), MK-9(H6) and MK-9(H4). The chemotaxonomic characteristics of this strain were consistent with those of the genus Streptoalloteichus. DNA-DNA hybridization, physiological, biochemical and chemotaxonomic data revealed that Streptomyces tenebrarius can be easily differentiated from the other member of the genus Streptoalloteichus and that it merits separate species status. The phenotypic and genetic evidence reveals that Streptomyces tenebrarius represents a novel species of the genus Streptoalloteichus; the name Streptoalloteichus tenebrarius (ex Higgins and Kastner) nom. rev., comb. nov. is proposed for this species. The type strain is NBRC
Acidothermus cellulolyticus ATCC ® 43068D-5™ Designation: Genomic DNA from Acidothermus cellulolyticus strain 11B TypeStrain=True Application:
Kineococcus radiotolerans is a radiation-resistant, motile, coccus-shaped, gram-positive bacterium with type strain SRS30216T (= ATCC BAA-149T = DSM 14245T). Phillips, R. W. (2002). Kineococcus radiotolerans sp. nov., a radiation-resistant, Gram-positive bacterium. International Journal of Systematic and Evolutionary Microbiology. 52 (3): 933-938. doi:10.1099/ijs.0.02029-0. ISSN 1466-5026. PMID 12054260. Ahmed, Niyaz; Bagwell, Christopher E.; Bhat, Swapna; Hawkins, Gary M.; Smith, Bryan W.; Biswas, Tapan; Hoover, Timothy R.; Saunders, Elizabeth; Han, Cliff S.; Tsodikov, Oleg V.; Shimkets, Lawrence J. (2008). Survival in Nuclear Waste, Extreme Resistance, and Potential Applications Gleaned from the Genome Sequence of Kineococcus radiotolerans SRS30216. PLoS ONE. 3 (12): e3878. doi:10.1371/journal.pone.0003878. ISSN 1932-6203. PMC 2587704 . PMID 19057647. Bagwell, C. E.; Milliken, C. E.; Ghoshroy, S.; Blom, D. A. (2008). Intracellular Copper Accumulation Enhances the Growth of Kineococcus ...
TY - JOUR. T1 - Genome of the actinomycete plant pathogen Clavibacter michiganensis subsp. sepedonicus suggests recent niche adaptation. AU - Bentley, Stephen D.. AU - Corton, Craig. AU - Brown, Susan E.. AU - Barron, Andrew. AU - Clark, Louise. AU - Doggett, Jon. AU - Harris, Barbara. AU - Ormond, Doug. AU - Quail, Michael A.. AU - May, Georgiana. AU - Francis, David. AU - Knudson, Dennis. AU - Parkhill, Julian. AU - Ishimaru, Carol A.. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2008/3. Y1 - 2008/3. N2 - Clavibacter michiganensis subsp. sepedonicus is a plant-pathogenic bacterium and the causative agent of bacterial ring rot, a devastating agricultural disease under strict quarantine control and zero tolerance in the seed potato industry. This organism appears to be largely restricted to an endophytic lifestyle, proliferating within plant tissues and unable to persist in the absence of plant material. Analysis of the genome sequence of C. michiganensis subsp. ...
The ability to deconstruct plant biomass without conventional pretreatment has made members of the genus Caldicellulosiruptor the target of investigation for the consolidated processing of lignocellulosic biomass to biofuels and bioproducts. These Gram-positive bacteria are hyperthermophilic anaerobes and the most thermophilic cellulolytic organisms so far described. They use both C5 and C6 sugars simultaneously and have the ability to grow well on xylan, a major component of plant cell walls. This is an important advantage for their use to efficiently convert biomass at yields sufficient for an industrial process. For commodity chemicals, yield from substrate is perhaps the most important economic factor. In an attempt to improve even further the ability of C. bescii to use xylan, we introduced two xylanases from Acidothermus cellulolyticus. Acel_0180 includes tandem carbohydrate-binding modules (CBM2 and CBM3) located at the C-terminus, one of which, CBM2, is not present in C. bescii. Also, ...
Kineococcus radiotolerans ATCC ® BAA-149D-5™ Designation: Genomic DNA from Kineococcus radiotolerans strain SRS30216 TypeStrain=True Application:
TY - CHAP. T1 - Order Pseudonocardiales. AU - Franco, Christopher. AU - Labeda, David. PY - 2014. Y1 - 2014. N2 - The order Pseudonocardiales is made of up a single family Pseudonocardiaceae as it now contains all the genera that belonged to the other family in the order, the Actinosynemmataceae. It also covers the suborder Pseudonocardineae which forms a distinct cluster between the Frankineae and Streptomycineae when members of the taxa are subjected to 16S rRNA gene phylogenetic analysis. The family has 26 genera Pseudonocardia, Actinoalloteichus, Actinokineospora, Actinomycetospora, Actinophytocola, Actinosynnema, Alloactinosynnema, Allokutzneria, Amycolatopsis, Crossiella, Goodfellowiella, Haloechinothrix, Kibdelosporangium, Kutzneria, Labedaea, Lechevalieria, Lentzea, Prauserella, Saccharomonospora, Saccharopolyspora, Saccharothrix, Sciscionella, Streptoalloteichus, Thermocrispum, Umezawaea, and Yuhushiella. These genera contain strains which are heterogenous in their morphological and ...
A polyphasic study was designed to determine the taxonomic provenance of three Modestobacter strains isolated from an extreme hyper-arid Atacama Desert soil. The strains, isolates KNN 45-1a, KNN 45-2b(T) and KNN 45-3b, were shown to have chemotaxonomic and morphological properties in line with their classification in the genus Modestobacter. The isolates had identical 16S rRNA gene sequences and formed a branch in the Modestobacter gene tree that was most closely related to the type strain of Modestobacter marinus (99.6% similarity). All three isolates were distinguished readily from Modestobacter type strains by a broad range of phenotypic properties, by qualitative and quantitative differences in fatty acid profiles and by BOX fingerprint patterns. The whole genome sequence of isolate KNN 45-2b(T) showed 89.3% average nucleotide identity, 90.1% (SD: 10.97%) average amino acid identity and a digital DNA-DNA hybridization value of 42.4±3.1 against the genome sequence of M. marinus DSM 45201(T), ...
The crude extracts of the fermentation broth from a marine sediment-derived actinomycete strain, Saccharothrix sp. 10-10, showed significant antibacterial activities against drug-resistant pathogens. A genome-mining PCR-based experiment targeting the genes encoding key enzymes involved in the biosynthesis of secondary metabolites indicated that the strain 10-10 showed the potential to produce tetracenomycin-like compounds. Further chemical investigation of the cultures of this strain led to the identification of two antibiotics, including a tetracenomycin (Tcm) analogs, Tcm X (1), and a tomaymycin derivative, oxotomaymycin (2). Their structures were identified by spectroscopic data analysis, including UV, 1D-NMR, 2D-NMR and MS spectra. Tcm X (1) showed moderate antibacterial activities against a number of drug-resistant pathogens, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) pathogens, with the MIC values in the range of 32-64 microg x ...
In September 2009, the University of Nebraska-Lincoln Plant and Pest Diagnostic Clinic received leaf samples of hybrid corn (Zea mays L.) displaying long, necrotic lesions with wavy margins. The lesions had discontinuous water-soaked spots that are indicative of Gosss bacterial wilt and leaf blight. The symptomatic leaves were submitted from Dallam County, located in the Texas Panhandle (northwest Texas). According to the USDA Farm Service Agency and the National Agricultural Statistics Service, in 2009 Dallam County had 54,025 ha planted to corn. This is approximately 19% of the total corn planted in the 26 counties in the Texas Panhandle and 6% of the total corn planted in the state of Texas. Extracts from the infected leaf tissue tested positive for Clavibacter michiganensis subsp. nebraskensis with a commercially available ELISA test (Neogen Inc., Scotland, UK). Isolation from the infected tissue onto CNS selective media (1) resulted in round, dark orange, mucoid colonies that tested gram ...
A novel actinomycete that was capable of degrading poly(l-lactic acid), strain CMU-PLA07T, was isolated from soil in northern Thailand. Strain CMU-PLA07T had biochemical, chemotaxonomic, morphological and physiological properties that were consistent with its classification in the genus Amycolatopsis. 16S rRNA gene sequence analysis showed that the isolate formed a phyletic line within the genus Amycolatopsis. Strain CMU-PLA07T was most similar to Amycolatopsis coloradensis IMSNU 22096T (99.5 % 16S rRNA gene sequence similarity) and Amycolatopsis alba DSM 44262T (99.4 %). However, strain CMU-PLA07T was distinguishable from the type strains of species of the genus Amycolatopsis on the basis of DNA-DNA relatedness and phenotypic data. Therefore, strain CMU-PLA07T is considered to represent a novel species of the genus Amycolatopsis, for which the name Amycolatopsis thailandensis sp. nov. is proposed. The type strain is CMU-PLA07T ( = JCM 16380T = BCC 38279T).
Plasmids were constructed using Q5 High-Fidelity DNA polymerase (New England BioLabs, Ipswich, MA, USA) for PCR reactions, restriction enzymes (New England BioLabs, Ipwich, MA, USA), and the Fast-link DNA ligase kit (Epicentre Biotechnologies, Madison, WI, USA) according to the manufacturers instruction. Plasmid pSKW10 (Fig. 1b) was constructed in two cloning steps (Additional file 1: Fig. S10). First, a 1.9 kb DNA fragment containing the coding sequence of Acel_0180 without the signal sequence was amplified with primers DC701 (with an ApaLI site) and DC702 (with an AvrII site) using A. cellulolyticus 11B gDNA as template. The 5.9 kb DNA fragment containing the regulatory region of Cbes2303 (P S-layer), the signal CelA signal sequence, a C-terminal 6X histidine tag, and a rho-independent transcription terminator was amplified by PCR with primers DC699 (with ApaLI site) and DC700 (with AvrII site) using pDCW212 as template. This 5.9 kb DNA fragment was also amplified from plasmid pDCW175 [10], ...
This directive contains the guidelines for the investigative procedure that must be followed upon the detection of Clavibacter Michiganensis on a seed potato farm unit.
1ECE: Crystal structure of thermostable family 5 endocellulase E1 from Acidothermus cellulolyticus in complex with cellotetraose.
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinoplanes utahensis.
Citation: Labeda, D.P., Kroppenstedt, R.M. 2008. Proposal for the new genus Allokutzneria gen. nov. within the suborder Pseudonocardineae and transfer of Kibdelosporangium albatum Tomita et al. 1993 as Allokutzneria albata comb. nov. International Journal of Systematic and Evolutionary Microbiology. 58:1472-1475. Interpretive Summary: Gene sequence-based molecular analyses for reassessment of evolutionary relationships of microoganisms at the species, genus, or higher taxonomic levels can sometimes reveal unexpected problems with existing classification of microbial species where conclusions based incomplete morphological or chemical composition data resulted in incorrect decisions. The microorganism that produces the antiviral antibiotic cycloviracin was described as a species of the genus Kibdelosporangium in 1993 based on data available to the researchers at that time. A molecular systematic analysis of the 16S ribosomal RNA gene sequences of all members of the bacterial family ...
A Gram-positive bacterium strain, designated TT02-04T, was isolated from a forest soil sample in Iriomote Island, Japan, and its taxonomic position was investigated by using a polyphasic approach. The peptidoglycan type of this organism was found to be A4α and lysine was the diagnostic cell-wall diamino acid of the peptidoglycan. The only menaquinone was MK-8(H4), and the major fatty acid was anteiso-C15 : 0. Galactose was detected as the cell-wall sugar. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides. The DNA G+C content was 73.0 mol%. 16S rRNA gene sequencing studies indicated that strain TT02-04T was closely related to the type strains of Georgenia ruanii (99.0 %) and Georgenia muralis (97.7 %). However, DNA-DNA hybridization results and phenotypic characteristics revealed that the strain differed from the currently recognized species of the genus Georgenia. Therefore, strain TT02-04T represents a novel species of the genus Georgenia, for
Citation: N/A Interpretive Summary: A previous study of a taxonomic group of soil bacteria indicated a strain which produces the novel antibiotic dopsisamine had been mis-classified at least twice. A detailed study of all of the properties of this strain, including morphology, chemical composition, and physiology, was undertaken to properly describe this new genus. The name Crossiella is proposed for this new genus in recognition of contributions of Thomas Cross, a distinguished microbiologist at the University of Bradford. The knowledge regarding the properties of this new genus will be valuable to biotechnologists searching for microorganisms capable of producing other new antibiotics or natural products. Technical Abstract: Phylogenetic analysis of the genera within the suborder Pseudonocardineae based on almost complete sequences of 16S rDNA showed that Saccharothrix cryophilis NRRL B-16238T was misplaced within the genus Saccharothrix. S. cryophilis NRRL B-16238T appeared to be ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form of acetate, derived from glycolysis and pyruvate oxidation for carbohydrates and from beta oxidation of fatty acids. The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. In a series of reactions two carbons in citrate are oxidized to CO2 and the reaction pathway supplies NADH for use in the oxidative phosphorylation and other metabolic processes. The pathway also supplies important precursor metabolites including 2-oxoglutarate. At the end of the cycle the remaining four-carbon part is transformed back to oxaloacetate. According to the genome sequence data, many organisms seem to lack genes for the full cycle [MD:M00009], but contain genes for specific segments [MD:M00010 M00011 ...
The sponge-associated actinomycetes were isolated from the marine sponge Dendrilla nigra, collected from the southwest coast of India. Eleven actinomycetes
Taxonomy of the species Kitasatospora azatica corrig. (Nakagaito et al. 1993) Zhang et al. 1997 and Kitasatosporia azatica (sic) (Nakagaito et al. 1993) Zhang et al. 1997
Sigma-Aldrich offers abstracts and full-text articles by [Alon Savidor, Laura Chalupowicz, Doron Teper, Karl-Heinz Gartemann, Rudolf Eichenlaub, Shulamit Manulis-Sasson, Isaac Barash, Guido Sessa].
The citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form of acetate, derived from glycolysis and pyruvate oxidation for carbohydrates and from beta oxidation of fatty acids. The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. In a series of reactions two carbons in citrate are oxidized to CO2 and the reaction pathway supplies NADH for use in the oxidative phosphorylation and other metabolic processes. The pathway also supplies important precursor metabolites including 2-oxoglutarate. At the end of the cycle the remaining four-carbon part is transformed back to oxaloacetate. According to the genome sequence data, many organisms seem to lack genes for the full cycle [MD:M00009], but contain genes for specific segments [MD:M00010 M00011 ...
TY - JOUR. T1 - Gene cloning and molecular characterization of an extracellular poly(L-lactic acid) depolymerase from Amycolatopsis sp. strain K104-1. AU - Matsuda, Emiko. AU - Abe, Naoki. AU - Tamakawa, Hideyuki. AU - Kaneko, Jun. AU - Kamio, Yoshiyuki. PY - 2005/11. Y1 - 2005/11. N2 - We have isolated a polylactide or poly(L-lactic acid) (PLA)-degrading bacterium, Amycolatopsis sp. strain K104-1, and purified PLA depolymerase (PLD) from the culture fluid of the bacterium. Here, we cloned and expressed the pld gene encoding PLD in Streptomyces lividans 1326 and characterized a recombinant PLD (rPLD) preparation. We also describe the processing mechanism from nascent PLD to mature PLD. The pld gene encodes PLD as a 24,225-Da polypeptide consisting of 238 amino acids. Biochemical and Western immunoblot analyses of PLD and its precursors revealed that PLD is synthesized as a precursor (prepro-type), requiring proteolytic cleavage of the N-terminal 35-amino-acid extension including the ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
A novel Gram-positive, aerobic, actinobacterial strain, CF6/1T, was isolated in 2007 during environmental screening of arid desert soil in the Sahara near to Ourba, Chad. The isolate was found to grow best in a temperature range of 20-37 °C and at pH 6.0-8.5 and showed no NaCl tolerance, forming black-coloured and nearly circular colonies on GYM agar. Chemotaxonomic and molecular characteristics determined for the isolate match those previously described for members of the genus Geodermatophilus. The DNA G + C content of the novel strain was determined to be 74.9 mol %. The peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid. The main phospholipids were determined to be phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, diphosphatidylglycerol and traces of phosphatidylglycerol; MK-9(H4) was identified as the dominant menaquinone and galactose as the diagnostic sugar. The major cellular fatty acids were found to be the branched-chain ...
SWISS-MODEL Repository entry for A0LRP7 (RS4_ACIC1), 30S ribosomal protein S4. Acidothermus cellulolyticus (strain ATCC 43068 / 11B)
SWISS-MODEL Repository entry for A0LQZ8 (SYC_ACIC1), Cysteine--tRNA ligase. Acidothermus cellulolyticus (strain ATCC 43068 / 11B)
ID STANL_1_PE2867 STANDARD; PRT; 458 AA. AC STANL_1_PE2867; D3Q9A8; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Drug resistance transporter, EmrB/QacA subfamily;Flags: DE Precursor; (STANL_1.PE2867). GN OrderedLocusNames=Snas_2915; OS STACKEBRANDTIA NASSAUENSIS DSM 44728. OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Glycomycineae; Glycomycetaceae; Stackebrandtia. OX NCBI_TaxID=446470; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS STANL_1.PE2867. CC Stackebrandtia nassauensis DSM 44728 chromosome, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:D3Q9A8_STANL CC -!- GENE_FAMILY: HOG000239124 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; D3Q9A8; -. DR EMBL; CP001778; ADD42590.1; -; Genomic_DNA. DR RefSeq; YP_003511683.1; NC_013947.1. DR GeneID; 8884114; -. DR GenomeReviews; CP001778_GR; Snas_2915. DR KEGG; sna:Snas_2915; -. DR GO; ...
Actinobacterium strain S63T isolated from a soil sample collected from Spindrift Col on Signy Island (South Orkney Islands, maritime Antarctic) is a new species of the Intrasporangiaceae family. Here we report a draft genome sequence with an approximate size of 5 Mbp contained in 54 contigs (69.33% GC content). Preliminary analysis revealed the presence of cold active protein coding sequences, which may indicate an adaptation to the harsh polar environment from which the strain was isolated ...
Phytopathology 101:349-357...Phytopathology 101:349-357...Silencing of Host Basal Defense Response-Related Gene Expression Increases Susceptibility of Nicotiana benthamiana to Clavibacter michiganensis subsp. michiganensis...
Streptosporangium cinnabarinum GE82832 peptide: a secondary metabolite produced by Streptosporangium cinnabarinum (strain GE82832) that is a translational inhibitor
Marcone, G.L.Nonomuraea sp. ATCC 39727 a rare actinomycete producing the glycopeptide A40926: mechanism of self resistance. (Doctoral Thesis, Università degli Studi dellInsubria, 2010 ...
Wink JM, Kroppenstedt RM, Ganguli BN, et al. 2003. Three new antibiotic producing species of the genus Amycolatopsis, Amycolatopsis balhimycina sp. Nov., Amycolatopsis tolypomycina sp. Nov., Amycolatopsis vancoresmycina sp. Nov., and description of Amycolatopsis keratiniphila subsp. Keratiniphila subsp. Nov. and Amycolatopsis keratiniphila subsp. Nogabecina subsp. Nov. Syst Appl Microbiol 26:38. PUBMED Abstract ...
TAN 999: has macrophage-activating properties; from Nocardiopsis dassonvillei; structure given in first source; indolocarbazole alkaloid
Complete genome sequence of Catenulispora acidiphila type strain (ID 139908T). Energy Technology Data Exchange (ETDEWEB). Copeland, A [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Glavina Del Rio, Tijana [U.S. Department of Energy, Joint Genome Institute; Nolan, Matt [U.S. Department of Energy, Joint Genome Institute; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Chen, Feng [U.S. Department of Energy, Joint Genome Institute; Tice, Hope [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Mikhailova, Natalia [U.S. Department of Energy, Joint Genome Institute; Pati, Amrita [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, ...
ACTINOMYCETALES (MIKROBIOLOGIE); ZELLDIFFERENZIERUNG + ZELLREIFUNG (CYTOLOGIE); REGULATION AUF MOLEKULARER EBENE (BIOLOGIE); ACTINOMYCETALES (MICROBIOLOGY); CELL DIFFERENTIATION + CELL MATURATION (CYTOLOGY); REGULATION ON MOLECULAR LEVEL (BIOLOGY ...
Are you a fan of prog metal bands like Between The Buried and Me or The Odious? Well, I have just the thing to whet your whistle. Bisporas first full length definitely has a lot of stuff going on! A little less jazz strut than BTBAM, but a bit more funk-esque groove, and even some technical death metal-ish riffs (like in the intro of the second preview track)! Try to buy the album if you can, Bispora is trying to be able to afford merch ...
Kibdelone B is a minor analogue of a potent antitumor complex isolated from Kibdelosporangium sp.. Structurally, kibdelone B is related to lysolipin and albofungin, however no comparative investigation of this class has been reported ...
Serum antibody titres to Dermatophilus congolensis demonstrated by an enzyme-linked immunosorbent assay (ELISA) in young steers and in adult cows from an Ayrshire herd showed a bimodal distribution and provided evidence of subclinical infection. Very high titres detected in sera from crossbred Galloway steers were indicative of recent or existing infection which may have been masked by concurrent ringworm. The ELISA is a sensitive and technically simple method which enables sera to be screened for evidence of infection by D congolensis which may otherwise pass unrecognised. Such infections may be of importance not only in the epidemiology of the disease in farm animals but also as a potential source of infection for man and his domestic pets.. ...
A novel halophilic actinomycete, strain H32T,was isolated froma Saharan soil sample collected in El-Oued province, south Algeria. The isolate was characterized by means of polyphasic taxonomy. Optimal growth was determined to occur at 28-32°C, pH 6.0-7.0 and in the presence of 15-25 %(w/v) NaCl. The strain was observed to produce abundant aerial mycelium, which formed long chains of rod-shaped spores at maturity, and fragmented substrate mycelium. The cell wall was determined to contain meso-diaminopimelic acid and the characteristic whole-cell sugars were arabinose and galactose. The predominant menaquinoneswere found to beMK-10(H4) andMK-9(H4). The predominant cellular fatty acids were determined to be anteiso C17:0, iso-C15:0 and iso-C16:0. The diagnostic phospholipid detected was phosphatidylcholine. Phylogenetic analyses based on the 16S rRNA gene sequence showed that this strain formed a distinct phyletic line within the radiation of the genus Actinopolyspora. The 16S rRNAgene sequence ...
FERRO, Maria Inês Tiraboschi et al. Differential gene expression in sugar cane infected by Leifsonia xyli subsp: xyli. Summa phytopathol. [online]. 2007, vol.33, n.2, pp.157-166. ISSN 0100-5405. http://dx.doi.org/10.1590/S0100-54052007000200010.. The macroarray nylon membrane technology was used to study the differential gene expression of 3.575 ESTs clones from sugarcane libraries. Total RNA was extracted from two varieties, one known as tolerant (SP80-0185) and another known as susceptible (SP70-3370) to the ratoon stunting disease (RSD), after being inoculated with Lefsonia xyli subsp. xyli bacterial extracts, and used as probe. Out of the 3.575 ESTs, 49 showed differential expression levels. Most of the selected ESTs were found in the resistant variety, 41 being induced and 3 being repressed in response to bacterial inoculation. On the other hand, the susceptible variety showed only 5 differentially expressed genes, 2 being induced and 3 being repressed. These results indicate that the ...
Drought tolerance of plants such as tomato or pepper can be improved by their inoculation with rhizobacteria such as Microbacterium sp. 3J1. This interaction depends on the production of trehalose by the microorganisms that in turn modulate the phyto-hormone profile of the plant. In this work we describe the characterization of metabolic changes during the interaction of pepper plants with Microbacterium sp. 3J1 and of the microorganism alone over a period of drought. Our main findings include the observation that the plant responds to the presence of the microorganism by changing the C and N metabolism based on its glutamine and alpha-ketoglutarate content, these changes contribute to major changes in the concentration of molecules involved in the balance of the osmotic pressure. These include sugars and amino-acids; the concentration of antioxidant molecules, of metabolites involved in the production of phytohormones like ethylene, and of substrates used for lignin production such as ferulic ...
Leifsonia xyli subsp. xyli, causal agent of ratoon stunting disease (RSD) of sugarcane (Saccharum interspecific hybrids), is the most well-known member of the Microbacteriaceae genus Leifsonia. However, the presence of other Leifsonia strains associated with sugarcane has not been reported. A total of 697 Australian and 40 Indonesian sugarcane fields were screened by leaf sheath biopsy (LSB) PCR using primers specific for L. xyli subsp. xyli, in addition to primers designed to amplify DNA from other members of the genus Leifsonia. While L. xyli subsp. xyli was detected in 126 fields, a total of 37 distinct and novel Leifsonia and non-Leifsonia strains were detected in 116 fields. Representatives of these strains were also detected in multiple samples of expressed xylem sap. Sequencing and phylogenetic analyses demonstrated the presence of a broad complex of novel Leifsonia strains, in addition to strains closely related to the recently erected Cnuibacter genus. Attempts to isolate Leifsonia strains were
Mud fever, also known as scratches or pastern dermatitis, is a group of diseases of horses causing irritation and dermatitis in the lower limbs of horses. Often caused by a mixture of bacteria, typically Dermatophilus congolensis, and Staphylococcus spp, mud fever can also be caused by fungal organisms (dermatophytes). Photosensitization, chorioptic mange mites, contact dermatitis and other conditions also contribute to some cases. This condition is also known as dew poisoning, grease heel, or greasy heel. Mud fever affects most horses and ponies during winter and early spring, resulting in painful sores and scabs, which in severe cases can make a horse lame. Mud fever most commonly affects the pastern and heel area but can also affect the upper leg, the belly, and in some cases the neck area (also known as Rain Scald). Non-pigmented skin tends to be more severely affected. Mud fever is caused by an infection of the skin by bacteria, including Dermatophilus congolensis, and often Staphylococcus ...
81957PRTAcidothermus cellulolyticusmisc_feature(957)..(957)Xaa can be any naturally occurring amino acid 1Met Asp Arg Ser Glu Asn Ile Arg Leu Thr Met Arg Ser Arg Arg Leu1 5 10 15Val Ser Leu Leu Ala Ala Thr Ala Ser Phe Ala Val Ala Ala Ala Leu 20 25 30Gly Val Leu Pro Ile Ala Ile Thr Ala Ser Pro Ala His Ala Ala Thr 35 40 45Thr Gln Pro Tyr Thr Trp Ser Asn Val Ala Ile Gly Gly Gly Gly Phe 50 55 60Val Asp Gly Ile Val Phe Asn Glu Gly Ala Pro Gly Ile Leu Tyr Val65 70 75 80Arg Thr Asp Ile Gly Gly Met Tyr Arg Trp Asp Ala Ala Asn Gly Arg 85 90 95Trp Ile Pro Leu Leu Asp Trp Val Gly Trp Asn Asn Trp Gly Tyr Asn 100 105 110Gly Val Val Ser Ile Ala Ala Asp Pro Ile Asn Thr Asn Lys Val Trp 115 120 125Ala Ala Val Gly Met Tyr Thr Asn Ser Trp Asp Pro Asn Asp Gly Ala 130 135 140Ile Leu Arg Ser Ser Asp Gln Gly Ala Thr Trp Gln Ile Thr Pro Leu145 150 155 160Pro Phe Lys Leu Gly Gly Asn Met Pro Gly Arg Gly Met Gly Glu Arg 165 170 175Leu Ala Val Asp Pro Asn Asn Asp Asn Ile Leu Tyr Phe Gly Ala Pro 180 185 190Ser Gly Lys Gly ...
The taxonomic position of an actinomycete isolated from a brown hypermagnesian ultramafic soil was examined using a polyphasic approach. The organism, which was designated SBHS Strp1T, was found to have chemical and morphological properties typical of Amycolatopsis strains. It was most closely associated with Amycolatopsis kentuckyensis, Amycolatopsis lexingtonensis, Amycolatopsis rifamycinica, Amycolatopsis pretoriensis and Amycolatopsis tolypomycina on the basis of 16S rRNA gene sequence data, and showed a unique pattern of phenotypic properties that distinguished it from the type strains of these taxa. The combined genotypic and phenotypic data show that the organism merits description as a novel species of Amycolatopsis. The name proposed for the novel species is Amycolatopsis plumensis sp. nov.; the type strain is SBHS Strp1T (=DSM 44776T=NRRL B-24324T).
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Other names: ATCC 25835, CFBP 4266, CIP 100331, Cellulomonas turbata, Cellulosimonas turbata, DSM 20577, IFO 15015, JCM 3160, LMG 4072, NBRC 15015, NCIB 10587, NCIMB 10587, NCTC 11973, NRRL B-8019, Nocardia turbata, O. turbata, Oerskovia turbata, strain 891, strain rskov 27 ...
Other names: ATCC 25835, CFBP 4266, CIP 100331, Cellulomonas turbata, Cellulosimonas turbata, DSM 20577, IFO 15015, JCM 3160, LMG 4072, NBRC 15015, NCIB 10587, NCIMB 10587, NCTC 11973, NRRL B-8019, Nocardia turbata, O. turbata, Oerskovia turbata, strain 891, strain rskov 27 ...
General Information: Amycolatopsis belongs to the actinomycete family Pseudonocardiaceae. Amycolatopsis mediterranei produces the antibiotic rifamycin, derivatives of which are used for curing tuberculosis and leprosy. Antibiotic producing bacterium. ...
ZEOCIN RESISTANCE. For Selecting Transfected Cells. To easily select cells that were transfected with our genetic circuit, we required a selectable marker that would work in all of our chassis, particularly HeLa cells and microglia, and would enable us to easily eliminate cells that have not taken up our recombinant plasmid. Zeocin is a widely used glycopeptide antibiotic, a formulation of phleomycin D1. It is capable of binding to and cleaving DNA, leading to cell necrosis in both eukaryotes and aerobic prokaryotes. Commonly outside of cells, in copper-chelated form, zeocin is inactive. When zeocin enters a cell, the Cu2+, which makes it appear blue, is reduced to Cu+ and then removed, activating zeocin, which then intercalates into DNA (Invitrogen). A 375 base pair bacterial gene encodes the Streptoalloteichus hindustanus bleomycin resistance protein (She ble protein). The She ble protein in mammalian cells is predominantly localised at the nucleus, specifically at euchromatin (Calmels et al. ...
ZEOCIN RESISTANCE. For Selecting Transfected Cells. To easily select cells that were transfected with our genetic circuit, we required a selectable marker that would work in all of our chassis, particularly HeLa cells and microglia, and would enable us to easily eliminate cells that have not taken up our recombinant plasmid. Zeocin is a widely used glycopeptide antibiotic, a formulation of phleomycin D1. It is capable of binding to and cleaving DNA, leading to cell necrosis in both eukaryotes and aerobic prokaryotes. Commonly outside of cells, in copper-chelated form, zeocin is inactive. When zeocin enters a cell, the Cu2+, which makes it appear blue, is reduced to Cu+ and then removed, activating zeocin, which then intercalates into DNA (Invitrogen). A 375 base pair bacterial gene encodes the Streptoalloteichus hindustanus bleomycin resistance protein (She ble protein). The She ble protein in mammalian cells is predominantly localised at the nucleus, specifically at euchromatin (Calmels et al. ...
Members of this protein family are restricted to members of the Actinobacteria (Mycobacterium smegmatis, Streptomyces hygroscopicus, Geodermatophilus obscurus, Pseudonocardia dioxanivorans, Saccharomonospora marina, etc) that synthesize PQQ. This small protein, 155 amino acids long on average, is found regularly next to a much larger protein, a PQQ-dependent oxidoreductase, and might be a companion subunit or an accessory protein such as chaperone involved in cofactor insertion ...
General Information: This strain was isolated from a diary herd in Wisconsin, USA in the 1970s. Environmental organism which causes infections in birds and humans. This genus comprises a number of Gram-positive, acid-fast, rod-shaped aerobic bacteria and is the only member of the family Mycobacteriaceae within the order Actinomycetales. Like other closely related Actinomycetales, such as Nocardia and Corynebacterium, Mycobacteria have unusually high genomic DNA GC content and are capable of producing mycolic acids as major components of their cell wall. Mycobacterium avium is ubiquitous in the environment, and can be found in stagnant waters and soils. This organism causes tuberculosis in birds and disseminated infections in immunocompromized humans (the elderly, children, and especially patients with AIDS). Infection results in a characteristic pulmonary disease which requires expensive drug therapy for successful treatment. Most prevalent colony morphotypes are smooth opaque, smooth ...
The Diseases of Elaeagnus. The Elaeagnus plant genus (Elaeagnus spp.) consists of large shrubs or small trees with bushy forms and silvery-gray foliage. These plants bear fragrant, light-colored flowers that give way to edible, red or yellow, olive-like fruits. Commonly grown plants in this genus include the Russian ...
Introduction: Elaeagnus spp. is one in the family of riparian trees growing near the rivers or water corridors. In this family, Elaeagnus angustifolia (Russian olive) is famous because of its medical applications. Methods: A comprehensive review was performed to extract the related data from published literature. Results: Traditionally, it has been used as an analgesic, antipyretic and diuretic herbal medicine. A large number of compounds have been derived from Russian olive and made this plant a source of flavonoids, alkaloids, minerals and vitamins. Although the purpose of most studies is to use this plant for preparation of herbal medicines and as an ingredient for drug formulation, there is no available drug dosage form commercially. Conclusion: This review aimed to provide the most important documentary information on the active components of Elaeagnus spp. and their relation to the pharmacological properties and compare them with reported medicinal effects.
16079331] Identification of tailoring genes involved in the modification of the polyketide backbone of rifamycin B by Amycolatopsis mediterranei S699. (Microbiology. , 2005 ...
Actinomycetes are gram-positive, free-living, saprophytic bacteria widely distributed in soil, water and colonizing plants showing marked chemical and morphological diversity. They are potential source of many bioactive compounds, which have diverse clinical effects and important applications in human medicine. In the present work, we have studied some of the physiological and biochemical characteristics of 36 actinomycete strains isolated from the shola soils of tropical montane forest; a relatively unexplored biodiversity hotspot. Ability of actinomycetes isolates to ferment and produce acids from various carbohydrate sources such as innositol, mannose, sorbitol, galactose, mannitol, xylose, rhamnose, arabinose, lactose and fructose were studied. Almost all the carbon compounds were utilized by one or other actinomycete isolates. The most preferred carbon sources were found to be xylose (94.44%) followed by fructose and mannose (91.66%). Only 41.76% of the isolates were able to ferment ...
Cell morphology of filamentous microorganisms is highly interesting during cultivations as it is often linked to productivity and can be influenced by process conditions. Hence, the characterization of cell morphology is of major importance to improve the understanding of industrial processes with filamentous microorganisms. For this purpose, reliable and robust methods are necessary. In this study, pellet morphology and physiology of the rebeccamycin producing filamentous actinomycete Lentzea aerocolonigenes were investigated by microscopy and flow cytometry. Both methods were compared regarding their applicability. To achieve different morphologies, a cultivation with glass bead addition (Ø = 969 μm, 100 g L-1) was compared to an unsupplemented cultivation. This led to two different macro-morphologies. Furthermore, glass bead addition increased rebeccamycin titers after 10 days of cultivation (95 mg L-1 with glass beads, 38 mg L-1 without glass beads). Macro-morphology and viability were
7-Chloro-8-methoxytetracycline isolated from antibiotic ES-119 and antibiotic Tet. 7 which are produced by fermentation of mutants of Actinomadura brunnea, namely Actinomadura brunnea var. antibiotica, ATCC 53108 and Actinomadura brunnea var antibiotica ATCC 53180 and its use as an antibiotic against gram-positive and gram-negative organisms are disclosed.
Pseudonocardia asaccharolytica sp. nov. and Pseudonocardia sulfidoxydans sp. nov., two new dimethyl disulfide-degrading actinomycetes and emended description of the genus Pseudonocardia ...
You searched for: All Fields sustainable agriculture Remove constraint All Fields: sustainable agriculture Topic crop rotation Remove constraint Topic: crop rotation Topic Zea mays Remove constraint Topic: Zea mays Topic tillage Remove constraint Topic: tillage Topic Actinomycetales Remove constraint Topic: Actinomycetales Topic Fusarium Remove constraint Topic: Fusarium Format Article OR Electronic Remove constraint Format: Article ,strong class=text-muted constraint-connector,OR,/strong, Electronic ...
The taxonomic position of a soil isolate, strain E626, was evaluated using the polyphasic approach. The organism was found to have chemical and morphological features consistent with its assignment to
Valid publication: PATHOM-AREE (W.), NOGI (Y.), WARD (A.C.), HORIKOSHI (K.), BULL (A.T.) and GOODFELLOW (M.): Dermacoccus barathri sp. nov. and Dermacoccus profundi sp. nov., novel actinomycetes isolated from deep-sea mud of the Mariana Trench. Int. J. Syst. Evol. Microbiol., 2006, 56, 2303-2307 ...
ID FRASU_1_PE451 STANDARD; PRT; 518 AA. AC FRASU_1_PE451; E3J9X7; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Drug resistance transporter, EmrB/QacA subfamily; DE (FRASU_1.PE451). GN OrderedLocusNames=FraEuI1c_0456; OS FRANKIA SP. EUI1C. OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Frankineae; OC Frankiaceae; Frankia. OX NCBI_TaxID=298654; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS FRASU_1.PE451. CC Frankia sp. EuI1c chromosome, complete genome. CC complete sequence. CC -!- ANNOTATIONS ORIGIN:E3J9X7_FRASU CC -!- GENE_FAMILY: HOG000239124 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; E3J9X7; -. DR EMBL; CP002299; ADP78539.1; -; Genomic_DNA. DR RefSeq; YP_004014409.1; NC_014666.1. DR GeneID; 9970870; -. DR GenomeReviews; CP002299_GR; FraEuI1c_0456. DR KEGG; fri:FraEuI1c_0456; -. DR GO; GO:0016021; C:integral to membrane; ...
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Lineage: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinobacteria; Streptosporangiales; Nocardiopsaceae; Streptomonospora; unclassified ...
Lineage: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis; Amycolatopsis ...
Actinomycetes also refers as filamentous Actinobacteria and acts as a connecting link between the bacteria and fungi as it shows resemblance with both. In this article, definition, characteristics, classification and economic importance of Actinomycetes are explained.
46. Elaeagnus mollis Diels, Bot. Jahrb. Syst. 36(Beibl. 82): 78. 1905. 翅果油树 chi guo you shu Trees or shrubs, deciduous, erect, 2-10 m tall, to 8 cm d.b.h., with grayish brown bark. Spines absent; young branches grayish green, densely stellate-scaly. Petiole 6-10 mm; leaf blade abaxially grayish green, adaxially dark green, ovate or ovate-elliptic, 6-9(-15) × 3-6(-11) cm, papery, abaxially densely grayish stellate-tomentose, adaxially sparsely stellate-hairy, lateral veins 6-10 per side of midrib, base obtuse or rounded, apex acute. Flowers often 1-3(-5) in leaf axils. Pedicel 3-4 mm. Flowers nodding, grayish green, fragrant, grayish white stellate-tomentose. Calyx tube campanulate, ca. 4 mm, abruptly constricted above ovary; lobes subtriangular or sublanceolate, 3.5-4 mm, inside sparsely white stellate-hairy, apex acuminate. Anthers ellipsoid, ca. 2 mm. Style erect, curved in upper part, lower part densely villous. Drupe globose-ovoid, or subglobose, ca. 1.3 cm, conspicuouly 8-ribbed, ...