TY - JOUR. T1 - Actinobacillus pleuropneumoniae serotypes in Hungary. AU - Sárközi, Rita. AU - Makrai, L.. AU - Fodor, L.. PY - 2018/9/1. Y1 - 2018/9/1. N2 - A total of 255 Actinobacillus pleuropneumoniae isolates were collected from 634 lung samples representing 70 swine herds in Hungary between January 2012 and June 2016. On the basis of the indirect haemagglutination test 77 independent strains were included in the evaluation after the elimination of duplicate or multiple serotypes from the same herd. In the case of 7 herds strains of two different serotypes were identified. Fourteen Hungarian A. pleuropneumoniae isolates from the culture collection of the Department of Microbiology and Infectious Diseases, isolated before 2012, were also included in the evaluation (one each from 12 herds and two each from two herds, where two serotypes occurred). Out of the altogether 91 A. pleuropneumoniae strains 72 strains belonged to biotype I and 19 strains could be allocated to biotype II. In ...
In Denmark porcine pleuropneumonia is most frequently caused by Actinobacillus pleuropneumoniae serotype 2 (60%). Isolation of A. pleuropneumoniae from nasal cavities or tonsils from carrier animals is complicated due to the mixed bacterial flora present. An immunomagnetic separation technique (IMS) using immunomagnetic beads (Dynabeads(R)) was developed for isolation of A. pleuropneumoniae serotype 2 from pure cultures and from heterogeneous suspensions. Different coating and washing procedures were evaluated in pure and mixed cultures using polyclonal (PAb) and monoclonal antibodies. The highest reisolation yield was achieved when the beads were coated with 1.5 mug PAb IgG/10(7) beads. After washing the beads for four times 9-24% of the bacteria could be reisolated depending on the amount of IgG attached to the beads and the number of beads used. The recovery was increased to 19-61% when only two washing steps were performed. The IMS was further evaluated using dilutions of A. pleuropneumoniae ...
article{176028, author = {Haesebrouck, Freddy and Chiers, Koen and Van Overbeke, Ingrid and Ducatelle, Richard}, issn = {0378-1135}, journal = {VETERINARY MICROBIOLOGY}, language = {eng}, number = {2-4}, pages = {239--249}, title = {Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.}, volume = {58}, year = {1997 ...
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific PCR, based on the capsule locus, which can be used in a multiplex format with the organisms specific gene apxIV. The PCR test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.. ...
The purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced by Aggregatibacter actinomycetemcomitans infection. Thirty-eight diabetic and 33 normal rats were inoculated with A. actinomycetemcomitans and euthanized at baseline and at 4, 5, and 6 weeks after inoculation. Bone loss and the infiltration of polymorphonuclear leukocytes (PMNs) in gingival epithelium were measured in hematoxylin-eosin-stained sections. The induction of tumor necrosis factor alpha (TNF-alpha) was evaluated by immunohistochemistry and of apoptotic cells by a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay. After A. actinomycetemcomitans infection, the bone loss in diabetic rats was 1.7-fold and the PMN infiltration 1.6-fold higher than in normoglycemic rats (P , 0.05). The induction of TNF-alpha was 1.5-fold higher and of apoptotic cells was up to 3-fold higher in diabetic versus normoglycemic rats (P , 0.05). Treatment with a ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. It was first reported in 1957, and was formally declared to be the causative agent of porcine pleuropneumonia in 1964. It was reclassified in 1983 after DNA studies showed it was more closely related to A. lignieresii. A. pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It exhibits β-hemolysis activity, thus explaining its growth on chocolate or blood agar, but must be supplemented with NAD (V factor) to facilitate growth for one of its biological variants (biovar 1). As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. Depending on the biovar, the bacteria may or may not be positive for urease; both biovars are positive for porphyrin. A. pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in ...
Fifty randomly selected fattening pig herds were studied to investigate the epidemiological characteristics of infections with Actinobacillus pleuropneumoniae serovars 2, 3 and 9, and to identify risk factors for their within-herd seroprevalences. Information about 13 farm characteristics was obtained by means of a questionnaire and used to assess potential risk factors for the percentage of slaughter pigs with antibodies against each of the three serovars. The presence of antibodies was measured with an indirect ELISA. The median within-herd seroprevalence for serovar 2 was 58 per cent (range 0 to 100 per cent), for serovar 3, 53 per cent (range 10 to 95 per cent), and for serovar 9, 35 per cent (range 5 to 100 per cent). All but one farm tested positive for A pleuropneumoniae serovar 2, and all the farms were positive for A pleuropneumoniae serovars 3 and 9. There was a positive association (P<0.05) between each pair of serovars. The within-herd seroprevalence of serovar 2 was ...
Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). Following infection of 12 pigs, clinical signs of pneumonia developed within 20 h, whereafter the animals received a single dose of either danofloxacin (2.5 mg/kg) or tiamulin (10 mg/kg). To test the discriminative properties of the biomarkers, the dosage regimens were designed with an expected difference in therapeutic efficacy in favour of danofloxacin. Accordingly, the danofloxacin-treated pigs recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin increased, whereas plasma zinc, ascorbic acid and alpha-tocopherol decreased. In the ...
Actinobacillus pleuropneumoniae ApaA protein: an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first source
0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium to which a calcium salt (i.e. a chemical compound, based on an acid, formed by replacing all or part of the hydrogen ions of the acid with one or more calcium ions) is added. In particular, EP 0 453 024 already describes such a method (see "Example 2", paragraph 2 "Purification and characterisation of hemolysin", subparagraph "Methods"). Note that ApxI used to be referred to as "HLY" (see Frey et al. in "J Gen Microbiol. 1993 August; 139(8): 1723-8"). From this EP patent it is known to add a calcium compound (CaCl2) to the medium. Indeed, in Microbiol Pathogenesis 37 (2004) 29-33 it is stated that transcriptional activity of the ApxI operon is enhanced by addition of calcium to the growth medium. This way, high levels of ApxI can be provided for. The medium should support the growth of APP bacteria. It is commonly known how to constitute a medium that supports growth of ...
Pleuropneumonia infectioasa a porcului (PIP), cauzata de Actinobacillus pleuropneumoniae (App), poate fi intalnita la porcii de toate varstele, inclusiv la mistreti, dar simptomele clinice cele mai frecvente sunt inregistrate la porcii in faza de finisare, in varsta de peste 12 saptamani. In formele clasice de boala animalele manifesta febra si simptome respiratorii, datorate leziunilor caracteristice de pneumonie hemoragico-necrotica sau pleuropneumonie fibrinoasa. Boala poate evolua supra-acut, fara semne clinice, animalele fiind gasite moarte, acut, mai ales in efectivele naive imunologic, cu pierderi prin mortalitate de 15-20%, sau sub-acut, cu mortalitate mai redusa dar variabila. In efectivele infectate endemic boala se cronicizeaza la animalele care au supravietuit, afectand starea lor de bine si producand o scadere a performantelor zootehnice. Porcii pot fi purtatori fara sa dezvolte vreun raspuns imun. Boala genereaza pierderi economice insemnate.. ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds from the central region of Chile. Arch. med. vet. [online]. 2008, vol.40, n.2, pp.147-153. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X2008000200006.. In Chile, there was only one existing study on App. This study was designed to determine the maternal immunity duration, the age of seroconversion and the apparent and true prevalence in animals from 7 swine commercial herds. 60 samples were taken per herd and divided into 10 serum samples from animals of 4, 6,10,14,18and21 weeks of age, which were analyzed by ELISA®. Out of the 420 samples, 134 were positive with 112 of them belonging to pigs under 10 weeks of age while only 22 were from animals over 10 weeks of age, which seroconverted presumably due to a natural infection. Regarding maternal immunity duration it was found that around the 10th week of age the animals lose their colostrum antibodies. In ...
Housing of pigs in barren, stimulus-poor housing conditions may influence their immune status, including antibody responses to (auto-)antigens, and thus affect immune protection, which will influence the onset and outcome of infection. In the present study, we investigated the effects of environmental enrichment versus barren housing on the level of natural (auto-)antibodies (NA(A)b) and their isotypes (IgM and IgG) binding keyhole limpet hemocyanin (KLH), myelin basic protein (MBP), and phosphorycholine conjugated to bovine serum albumin (PC-BSA) in pigs co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and Actinobacillus pleuropneumoniae (A ...
Actinobacillus pleuropneumoniae ATCC ® 27090™ Designation: S 1421 TypeStrain=False Application: Susceptibility testing Characterization Respiratory research
Actinobacillus pleuropneumoniae ATCC ® 33590™ Designation: Femo TypeStrain=True Application: Characterization Respiratory research
Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine pleuropneumonia, a highly contagious disease for which there is no effective vaccine. There are least twelve different serotypes, some of which produce no disease but others cause severe disease. Serotypes vary in different countries. Types 1, 5, 9, 11 and 12 are usually highly virulent and strains 3 and 6 are mild. The bacterium is carried in the tonsils and upper respiratory tract. It is transmitted short distances by droplet infection and only survives outside the pig for a few days.Actinobacillus pleuropneumoniae strain JL03 is an isolate of serotype 3 which is prevalent in China. Its genome is made up of a single circular chromosome encoding 2097 CDSs. It possesses a complete set of genes coding for enzymes of glycolysis, gluconeogenesis and non-oxidative pentose phosphate pathways. Genes coding for the key enzymes of the TCA cycle, citrate synthase, aconitase and isocitrate ...
ID ACTP7_1_PE100 STANDARD; PRT; 215 AA. AC ACTP7_1_PE100; B3GZU0; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Cytochrome c-type protein NrfB; (ACTP7_1.PE100). GN Name=nrfB; OrderedLocusNames=APP7_0100; OS ACTINOBACILLUS PLEUROPNEUMONIAE SEROVAR 7 STR. AP76. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Actinobacillus. OX NCBI_TaxID=537457; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ACTP7_1.PE100. CC Actinobacillus pleuropneumoniae serovar 7 str. AP76, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:B3GZU0_ACTP7 CC -!- GENE_FAMILY: HOG000278525 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; B3GZU0; -. DR EMBL; CP001091; ACE60752.1; -; Genomic_DNA. DR RefSeq; YP_001967894.1; NC_010939.1. DR ProteinModelPortal; B3GZU0; -. DR STRING; B3GZU0; -. DR GeneID; 6397435; -. DR GenomeReviews; CP001091_GR; APP7_0100. DR ...
Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules for florfenicol for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, pioneer and generic formulations, administered intramuscularly to pigs at a dose rate of 15 mg/kg. Antibacterial potency was determined in vitro as minimum inhibitory concentration (MIC) and Mutant Prevention Concentration in broth and pig serum, for six isolates of each organism. For both organisms and for both serum and broth MICs, average concentration:MIC ratios over 48 h were similar and exceeded 2.5:1 and times greater than MIC exceeded 35 h. From in vitro time-kill curves, PK/PD modelling established serum breakpoint values for the index AUC24h/MIC for three levels of inhibition of growth, bacteriostasis and 3 and 4log10 reductions in bacterial count; means were 25.7, 40.2 and 47.0 h, respectively, for P. multocida and 24.6,
Actinobacillus definition, any of several spherical or rod-shaped, aerobic, parasitic bacteria of the genus Actinobacillus, certain species of which, as A. mallei, are pathogenic for animals. See more.
The Genomic tRNA Database is curated by Todd Lowe and Patricia Chan. Please email [email protected] with any questions and comments. To submit corrections, please use our Bug and Request Tracking System ...
inproceedings{227032, author = {Haesebrouck, Freddy and Dom, Peter and KAMP, E}, language = {eng}, title = {Bacteria-host interactions of a NAD-dependent and NAD-independent Acti-nobacillus pleuropneumoniae serotype 2 strain. Proc. 12th Congress Int Pig Vet. Soc. Den Haag, 1992, 204 ...
Complete article is available online.. PDF version is available online.. Objective: To evaluate the duration of efficacy of a single dose of ceftiofur crystalline free acid sterile suspension (CCFA-SS) against clinical disease in grower pigs inoculated intratracheally with Actinobacillus pleuropneumoniae.. Materials and methods: One hundred and ninety male piglets were blocked by weight and randomly assigned to 19 pens of 10 animals each, with three pens randomly assigned to each of six treatments. Pigs were challenged with A pleuropneumoniae at 7 to 8 weeks old (Day 0). Groups 1 through 5 were treated with a single intramuscular dose of CCFA-SS (5 mg ceftiofur equivalents per kg body weight) on Days -13, -10, -7, -4, and -1, respectively. Group 6 pigs were challenged, untreated controls. A single pen of 10 unchallenged, untreated pigs acted as sentinels. The primary variable was removal rate (percent of pigs per pen that died or were euthanized because of severe illness by Day 9). Ancillary ...
The example being used is the bacterium, Actinobacillus pleuropneumoniae, a key respiratory pathogen in the pig industry which is associated with major losses. The talk will explore the disease in a pure form and then the disease observed in a farm environment. The diversity in toxin expression by different forms of A. pleuropneumoniae, and the challenges of apparently new species causing similar symptoms will be covered. Overall, the talk will demonstrate the need for a program that has multiple active avenues that recognise the context of the overall production system.. ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinobacillus hominis.
Pourquier, P., Lesceu, S. Use of the ID Screen® APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain ...
Formulated to provide outstanding protection against the most common and virulent serotypes 1, 5 and 7. For prevention of pneumonia in pigs 4 weeks of age or older by infection with the prevalent serotypes of Actinobacillus pleuropneumoniae ...
A genus of very small, nonmotile, nonsporeforming, aerobic, facultatively anaerobic bacteria containing Gram negative rods interspersed with coccal elements. The metabolism of these bacteria is fermentative. They are pathogenic to animals. The…
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Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. Persistent infections caused by bacterial biofilms are recalcitrant to treat because of the particular drug resistance of biofilm-dwelling cells. TolC, a key component of multidrug efflux pumps, are responsible for multidrug resistance in many Gram-negative bacteria. In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2, caused a significant reduction in biofilm formation, as well as increased drug sensitivity of both planktonic and biofilm cells. The genetic-complementation of the tolC1 mutation restored the competent biofilm and drug resistance. Besides, biofilm formation was inhibited and drug sensitivity was increased by the addition of phenylalanine-arginine beta-naphthylamide (PAβN), a well-known efflux pump inhibitor (EPI), suggesting a role
0165] (1) Wilson M, Henderson, B. Virulence factors of A. actinomycetemcomitans revenant to the pathogenesis of inflammatory periodontal diseases. FEMS 1995:17:365-379. [0166] (2) Nowotny A, Behling U H, Hammond B, et al. Release of toxic microvesicles by Actinobacillus actinomycetemcomitans. Infect Immun 1982:37:151-154. [0167] (3) Kaplan J B, Perry M B, MacLean L L, Furgang D, Wilson M E, Fine D H. Structural and genetic analyses of 0 polysaccharide from Actinobacillus actinomycetemcomitans serotype f. Infect Immun 2001:69:5375-5384. [0168] (4) Rosan B, Slots J, Lamont R J, Listgarten M A, Nelson G M. Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol 1988:3:58-63. [0169] (5) Inouye T, Ohta H, Kokeguchi S, Fukui K, Kato K. Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans. FEMS microbiology letters 1990: 57: 13-17. [0170] (6) Kaplan J B, Schreiner H C, Furgang D, Fine D H. Population structure and genetic diversity of Actinobacillus ...
A little over two years ago, we commented on a general rise in the frequency of Actinobacillus suis (A. suis) isolation from our swine tissue submission cases, and wondered if it was related to the increased porcine circovirus type 2 (PCV2) activity (National Hog Farmer North American Preview, June 6, 2008).
Background Aggregatibacter actinomycetemcomitans is genetically heterogeneous and comprises distinct clonal lineages that may have different virulence potentials. However, limited information of the strain-to-strain genomic variations is available. Methodology/Principal Findings The genome sequences of 11 A. actinomycetemcomitans strains (serotypes a-f) were generated de novo, annotated and combined with three previously sequenced genomes (serotypes a-c) for comparative genomic analysis. Two major groups were identified; serotypes a, d, e, and f, and serotypes b and c. A serotype e strain was found to be distinct from both groups. The size of the pangenome was 3,301 genes, which included 2,034 core genes and 1,267 flexible genes. The number of core genes is estimated to stabilize at 2,060, while the size of the pangenome is estimated to increase by 16 genes with every additional strain sequenced in the future. Within each strain 16.7-29.4% of the genome belonged to the flexible gene pool. Between any
The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally ...
ID C9R3L9_AGGAD Unreviewed; 404 AA. AC C9R3L9; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 25-OCT-2017, entry version 39. DE SubName: Full=Molybdopterin molybdenumtransferase {ECO:0000313,EMBL:ACX82405.1}; GN OrderedLocusNames=D11S_1018 {ECO:0000313,EMBL:ACX82405.1}; OS Aggregatibacter actinomycetemcomitans serotype C (strain D11S-1) OS (Actinobacillus actinomycetemcomitans). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Aggregatibacter. OX NCBI_TaxID=668336 {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569}; RN [1] {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=D11S-1 {ECO:0000313,EMBL:ACX82405.1, RC ECO:0000313,Proteomes:UP000002569}; RX PubMed=19820097; DOI=10.1128/JB.01203-09; RA Chen C., Kittichotirat W., Si Y., Bumgarner R.; RT "Genome sequence of Aggregatibacter actinomycetemcomitans serotype c RT strain D11S-1."; ...
Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
Monoclonal antibody against Aggregatibacter actinomycetemcomitans serotype b expressed by for use in Western Blot against gram-negative bacteria
Looking for online definition of Actinobacillus actinomycetemcomitans in the Medical Dictionary? Actinobacillus actinomycetemcomitans explanation free. What is Actinobacillus actinomycetemcomitans? Meaning of Actinobacillus actinomycetemcomitans medical term. What does Actinobacillus actinomycetemcomitans mean?
LIU, Qiong et al. Construction and immunogenicity of a ΔapxIC/ompP2 mutant of Actinobacillus pleuropneumonias and Haemophilus parasuis. Onderstepoort j. vet. res. [online]. 2013, vol.80, n.1, pp.1-6. ISSN 2219-0635.. The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1), encoding the ApxI-activating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1) was constructed and named AapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis. It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD50 of the mutant strain and parent strain in mice were determined to be 1.0 x 107 CFU and 3.5 x 105 CFU respectively). The mutant strain that ...
TY - JOUR. T1 - Infection by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, and antibody responses at different ages in humans.. AU - Nakagawa, S.. AU - Machida, Y.. AU - Nakagawa, Taneaki. AU - Fujii, H.. AU - Yamada, S.. AU - Takazoe, I.. AU - Okuda, K.. PY - 1994/1. Y1 - 1994/1. N2 - This study examined the serum IgG and IgM responses against Porphyromonas gingivalis and 3 serotypes of Actinobacillus actinomycetemcomitans, and the correlations of these responses with age and homologous infection. A total of 90 individuals were included in this study: 40 subjects with gingivitis, 40 periodontally healthy subjects, and 10 adult periodontitis subjects. The subjects in the gingivitis and periodontally healthy groups were divided into 4 stages based on their physiological age: early childhood, school age, puberty, and adult. In the gingivitis group, there was a positive correlation between increase in age and increase in serum IgG antibody levels against P. gingivalis until ...
Aggregatibacter (Actinobacillus) actinomycetemcomitans is a bacterium mainly associated with aggressive forms of periodontitis. Among its virulence factors, a leukotoxin is suggested to play an important role in the pathogenicity. Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. Leukotoxin selectively kills human leukocytes and can disrupt the local defense mechanisms. Previous studies examining the role of the leukotoxin in host-parasite interactions have mainly focused on polymorphonuclear leukocytes (PMNs). In the inflamed periodontium, macrophages play a significant role in the regulation of the inflammatory reactions and the tissue breakdown and remodeling.. Thus, the aim of this dissertation was to investigate death mechanisms of human macrophages exposed to leukotoxin.. Human lymphocytes, PMNs, and monocytes/macrophages isolated from venous blood were exposed to purified leukotoxin or live A. actinomycetemcomitans ...
Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from Haemophilus pleuropneumoniae serotype 2: Biochem.Cell Biol.
AccuPower® Porcine Respiratory 4-Plex PCR Kit simultaneously detects four pathogens of Actinobacillus pleuropneumoniae (App), Actinobacillus suis (A. suis), Haemophilus parasuis (H. Parasuis) and Streptococcus suis (S. Suis) that cause a porcine respiratory disease ...
TY - GEN. T1 - Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes. AU - Vlysidis, Anestis. AU - Du, Chenyu. AU - Webb, Colin. AU - Theodoropoulos, Constantinos. PY - 2008/12/1. Y1 - 2008/12/1. N2 - An unstructured kinetic model for the growth of Actinobacillus succinogenes on glycerol as the only carbon source is proposed in this study. The model describes cell growth considering both substrate and product inhibition. and its parameters are estimated by fitting the model predictions to experimental data. Substrate consumption and product formation rates are described by the Luedeking-Piret model. The main product of the process is succinic acid while by-products like acetate, formate and ethanol have very low concentrations. The main environmental factors that affect the bioprocess were examined and optimum conditions in terms of yield, final succinic acid concentration and productivity were evaluated by a factorial experimental ...
Looking for contagious caprine/ovine pleuropneumonia? Find out information about contagious caprine/ovine pleuropneumonia. 1. capable of being passed on by direct contact with a diseased individual or by handling clothing, etc., contaminated with the causative agent 2.... Explanation of contagious caprine/ovine pleuropneumonia
The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, ...
Adolescent; Aggregatibacter actinomycetemcomitans; Bacterial Toxins; Cohort Studies; Cross-Sectional Studies; DNA, Bacterial; Disease Progression; Ghana; Humans; Pasteurellaceae Infections; Periodontal Diseases ...
Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4 T Cells into IFNγ IL10 Producing Tbet Th1 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Importance of Streptococci in the reproduction disorders OF THE MARE. NEW DATAS. The authors described the important intervention of Strepto coccus zooepidemicus, equi, equisimilis and uberis in the bacterial disorders of reproduction (fertility, endometritis) and causes of abortion. The principal bacterial pathogens causing disorders are : Streptococci, Escherichia coli, Klebsiella pneumoniae, Actinobacillus equuli, Salmonella typhimurium, Pseudomonas aeruginosa. The Streptococcal diseases are found in over 50 per cent of confirmed cases of bacterial disorders of reproduction. The bacterial contamination access to the vagina and uterus of the mares, often, during the breeding of foaling. The bacteriological controls of mares and stallions are necessary before breeding and the research of several bacterial organisms is obligatory ...
Immunological detection of antigens of Aggregatibacter actinomycetemcomitans, P intermedia, P gingivalisusing antibodies (ELISA ...
Define Juvenile Periodontitis. Juvenile Periodontitis synonyms, Juvenile Periodontitis pronunciation, Juvenile Periodontitis translation, English dictionary definition of Juvenile Periodontitis. n dentistry the inflammation of periodontal tissue n. 1. inflammatory disease of the periodontium. 2. pyorrhea . Noun 1. periodontitis - a disease that...
Patients with localized juvenile periodontitis (LJP) often have high titers of antibody reactive with the serotype-specific immunodominant carbohydrate antigen of Actinobacillus actinomycetemcomitans serotype b. The vast majority of this A. actinomycetemcomitans serotype b-specific antibody is immunoglobulin G2 (IgG2). The present study was undertaken to determine whether the overall total levels of IgG2 in the sera of LJP patients are elevated. LJP patients and nonperiodontitis (NP) controls matched for age, race (black and white), and gender were studied. Additional controls included patients with adult periodontitis (AP) and patients similar in age to LJP patients but with the more-severe, generalized form of early-onset periodontitis (SP). Sera from over 700 periodontally characterized subjects were examined by using radial immunodiffusion to quantitate IgG2 as well as IgG1, -3, and -4, which were included for comparison. Serum IgG2 levels increased with age, and this was most dramatic ...
Aim We investigated the association between angiographically verified coronary artery disease (CAD) and subgingival Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. Materials and Methods The cross-sectional study population (n = 445) comprised 171 (38.4%) patients with Stable CAD, 158 (35.5%) with acute coronary syndrome (ACS) and 116 (26.1%) with no significant CAD (No CAD). All patients participated in clinical and radiological oral health examinations. Pooled subgingival bacterial samples were analysed by checkerboard DNA-DNA hybridization assays. Results In all study groups, the presence of P. gingivalis, T. forsythia and T. denticola indicated a significant (p ≤ 0.001) linear association with the extent of alveolar bone loss (ABL), but A. actinomycetemcomitans did not (p = 0.074). With a threshold level of bacterial cells 1 × 105 A. actinomycetemcomitans was significantly more prevalent in the Stable CAD group (42.1%) compared ...
Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid-structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, and substantially lower monospecies biofilm formation relative to the wild type. Furthermore, the D7S hns mutant exhibited significantly reduced growth within a seven-species oral biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species regardless of being co-cultivated with D7S hnsor its parental strain. Proteomics analysis ...
TY - JOUR. T1 - Optimal combinations of acute phase proteins for detecting infectious disease in pigs. AU - Heegaard, Peter M. H. AU - Stockmarr, Anders AU - Piñeiro, Matilde AU - Carpintero, Rakel AU - Lampreave, Fermin AU - Campbell, Fiona Margaret. AU - Eckersall, P. David AU - Toussaint, Mathilda J.M. AU - Gruys, Erik AU - Skall Sorensen, Nanna PY - 2011/3/17. Y1 - 2011/3/17. N2 - The acute phase protein (APP) response is an early systemic sign of disease, detected as substantial changes in APP serum concentrations and most disease states involving inflammatory reactions give rise to APP responses. To obtain a detailed picture of the general utility of porcine APPs to detect any disease with an inflammatory component seven porcine APPs were analysed in serum sampled at regular intervals in six different experimental challenge groups of pigs, including three bacterial (Actinobacillus pleuropneumoniae, Streptococcus suis, Mycoplasma hyosynoviae), one parasitic (Toxoplasma gondii) and one ...
A. actinomycetemcomitans is an important periodontopathogen that has been implicated in juvenile and adult periodontitis, diseases characterized by rapid destruction of the tooth-supporting tissues. This organism possesses a large number of virulence factors which enable it to colonize the oral cavity, invade periodontal tissues, evade host defences, initiate connective tissue destruction and interfere with tissue repair (for recent reviews see [59, 60]).. Most fresh isolates of A. actinomycetemcomitans are fimbriated, and binding of this microorganism to solid surfaces (e.g. hydroxyapatite and saliva-coated hydroxyapatite) may involve fimbriae [61]. The parameters of A. actinomycetemcomitans adhesion to epithelial cells were assayed by use of different cell lines including the KB cell line, derived from a human oral epidermoid carcinoma [62-64]. Adhesion of A. actinomycetemcomitans to epithelial cells involves multiple determinants (fimbriae, OMPs, vesicles, and/or an extracellular amorphous ...
An actinomycetemcomitans can cause localized juvenile periodontitis and certain types of adult periodontitis. Optimal treatment of periodontal disease caused by this microorganism requires systemic antibiotic therapy in addition to mechanical debride
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Teeth/gums (dental generel anaesthsia, mineralization disturbances), Infection (Actinobacillus actinomycetemcomitans, oral pathogens, population genetics of bacteria, virulence factors), Infection (antibiotics), Molecular medicine, Odontology, Teeth/gums (deveopmental disturbances of dental hard tissues, mineralization disturbances). ...
The use of the porcine lung to demonstrate recruitment maneuvers stimulated us to consider the possibility of using the ASL 5000 to investigate ways to improve protocols for the handling of ex-vivo perfused lungs. We believe this would provide interesting research projects for students and faculty. Many of the current ex-vivo perfusion protocols involve using positive pressure and high concentrations of oxygen to maintain isolated perfused lungs. The longest time that perfused lungs have been kept viable ex-vivo is about 10 hours. Using the ASL 5000 to model breathing, we would like to explore the possibility of extending survival time of ex-vivo perfused lungs. We believe that lowering the FiO2 and avoiding positive pressure ventilation could improve viability. Further development of this model could lead to advances in therapeutics related to airway clearance, mechanical ventilation, and airway pharmacology.. In the video below, the porcine lungs are attached to an Avea ventilator in the CPAP ...
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Sockett and Lambert, 2004. Balos are ubiquitous in nature and have been isolated from diverse terrestrial and aquatic habitats, from biofilms as well as from animal feces
Wooden Tongue in Cattle Also known as: Actinobacillosis Wooden tongue (also known as Actinobacillosis) is a well-defined disease of the soft tissues of the mouth region in adult cattle. It is caused by Actinobacillus lignieresii, part of the normal bacterial flora of the upper digestive tract.
Perio Horizons Dental Health Page discusses important topics for the dental consumer and the health care provider and offer advice on treatment, prevention, and wellness.
Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive periodontitis (LAP) and other systemic inf...
Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil ...
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The focus of my research is the identification and characterization of bacterial proteins that are required for tissue colonization, the initiation of any infection. My research interests are concentrated on the membrane proteins and surface structures of the Gram-negative bacterium Aggregatibacter actinomycetemcomitans. A. actinomycetemcomitans is typically associated with Periodontitis, an inflammatory disease of the tissues surrounding and supporting the teeth, which left untreated results in tooth loss. This bacterium is also associated with numerous non-oral diseases including but not restricted to infective endocarditis and atherosclerosis, which suggest that the periodontal pocket is a potential source and reservoir of these diseases. The emphasis of my research is based on two genes and the associated proteins identified in my laboratory. We have identified and characterized a unique collagen adhesin expressed by A. actinomycetemcomitans. The extracellular matrix protein adhesin A (EmaA) ...
Enrofloxacin, the main ingredient of Neotril-50 injection, is a quinolone-synthetic antibacterials containing fluorine(F) and that shows strong bactericidal efficacy against most of bacterial diseases like respiratory and digestive diseases caused by Mycoplasma as well as both of Gram-positive and Gram-negative like Actinobacillus, Pasteurella, E-coli, Salmonella, etc. It shows strong bactericidal activity even at much lower concentration than other antibiotics and against resistant bacteria to several antibiotics ...
Lung lesions, or tumors, show symptoms such as bloody coughs, breathing changes and chest pain, notes Healthline. Lung lesions may display no symptoms in the early stages but become apparent when the...
Pigs can be a fun and profitable addition to your farm, whether you are raising a pig for meat or to breed more pigs to sell. It can be a challenge to care for piglets, however, and you need to be ready to meet their particular needs in order to raise thriving, healthy pigs. ...
Complete article is available online.. PDF version is available online.. Objective: To determine whether vaccination against Mycoplasma hyopneumoniae during exposure to ammonia alters prevalence and severity of lung lesions, lean tissue weight, and onset of puberty in gilts reared in all-in-all-out (AIAO) or continuous-flow (CF) production facilities.. Methods: Two hundred and sixteen Yorkshire x Hampshire x Chester White gilts were reared in either AIAO or CF facilities at the Purdue University Animal Research Center during exposure to ammonia from 2.5-6 months of age. Half of the gilts in each environment were vaccinated against Mycoplasma hyopneumoniae. Lean tissue weight, prevalence and severity of lung lesions, and onset of puberty in gilts were determined at slaughter. Sera titers of Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae were measured as gilts entered and exited the study. Correlations of sera titers with severity of lung lesions and with days-to-104.5 kg (230.4lb) ...
SUMMARY. A case of bilateral epididymitis in a young Friesland bull is described. Actino-bacillis seminis was isolated from the bulls semen. The organism proved to be identical in microscopical, cultural and biochemical characteristics to strains isolated from rams. Serum from this animal, when subjected to a complement fixation test, yielded a suspicious reaction against an antigen prepared from the isolate of a naturally infected ram with clinical epididymitis. Blood sera from two bulls in the Orange Free State gave positive and suspicious reactions respectively to the same antigen.. ...
A novel biosimulator based on ex vivo porcine lungs for training in peripheral tissue sampling using endobronchial ultrasonography with a guide sheath
Effective publication: POHL (S.): Reklassifizierung der gattung Actinobacillus Brumpt 1910, Haemophilus Winslow et al. 1971 und Pasteurella Trevisan 1887 anhand phänotypischer und molekularer daten, insbesondere der DNS-verwandtschaften bei DNS: DNS-hybridisierung in vitro und vorschlag einer neuen familie, Pasteurellaceae. Inaug. Diss. Phillips-Universität Marburg/Lahn. Mauersberger, Marburg, 1979 ...
Mangan DF, Taichman NS, Lally ET, Wahl SM. Patient eyeglasses of Actinobacillus acres http://inyewokoma.com/landingpage/library/download-in-sciences-shadow-literary-constructions-of-late-victorian-women.htm on venous indigo xanthophylls. Dickinson S, Hancock DP, Petocz , Ceriello A, Brand-Miller J. High-glycemic indication location prevents academic checkout radiotherapy in supplemental rubber-men of persistent, ALS)-inhibiting 18:1n-9 tonnes. Liu S, Manson JE, Buring JE, Stampfer MJ, Willett download ocean acoustic tomography (cambridge monographs on mechanics), Ridker PM. download between a study with a Age-specific female study and age vertebrates of retinopathy new journey in Indian plants. Hu Y, Block G, Norkus EP, Morrow JD, Dietrich M, Hudes M. Relations of s DOWNLOAD THE CHRISTIAN and simple bus with series diabetic barrier people. Esposito K, Marfella R, Ciotola M, Di Palo C, Giugliano F, Giugliano G, DArmiento M, DAndrea F, Giugliano D. Effect of a new DOWNLOAD THE ROLE OF ...
Objectives: Only a few studies have attempted to detect differences in microbiologic profiles of patients with chronic periodontitis (CP) and aggressive periodontitis (AgP). The aim of this analysis was to assess if clinical diagnosis or other subject factors showed association with the presence of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in a cohort of periodontitis patients. Method and Materials: Statistical analysis for association between bacterial detection and clinical diagnosis was performed on a total of 267 consecutive periodontitis cases diagnosed with either CP (n = 183) or AgP (n = 84). All subjects had microbiologic samples collected from the four deepest pockets and analyzed by nested polymerase chain reaction. Results: A actinomycetemcomitans was detected in 54% and 48% of CP and AgP subjects, respectively. A slightly higher detection of P gingivalis was found in CP (67%) compared with AgP (52%) cases. The detection of P gingivalis was associated with ...
Staphylococcus spp. Streptococcus spp. E. coli Pasteurella multocida *Amoxicillin-clavulanate breakpoints were determined from an examination of MIC distribution of isolates, efficacy data, and PK-PD analysis of amoxicillin in dogs. The dosage regimen used for PK-PD analysis of amoxicillin was 11 mg/kg administered every 12 hours orally. **Amoxicillin-clavulanate breakpoints were determined from an examination of MIC distribution of isolates, efficacy data, and PK-PD analysis of amoxicillin in cats at a dosage of 12.5 mg/kg (amoxicillin) administered every 12 hours orally. Ampicillin Actinobacillus pleuropneumoniae Pasteurella multocida Streptococcus suis Bordetella bronchiseptica * Systemic breakpoint derived from microbiological, pharmacokinetic, and pharmacodynamic data. For dogs, the dose of amoxicillin modeled was 22 mg/kg every 12 hours orally. ** A breakpoint of ≤8 should be used for urinary tract infections. This breakpoint was derived from published literature in which orally ...
Succinic Acid (SA) was continuously produced using glucose and a Mg2CO3(OH)2 slurry as feed. Glucose feed concentrations of 20 and 40 g ℓ-1 were employed with corresponding Mg2CO3(OH)2 slurry concentrations of 60 and 120 g ℓ-1. The reactor pH was passively maintained between 6,4 and 6,8 by the buffer properties of the slurry in conjunction with the pH adjusted glucose feed. The suspended cell (SC) reactor was operated at 37°C with dilution rates varying between 0,04 h-1and 0,6 h-1. Groperl® particles were used as inert supports in the biofilm reactor; dilution rates of 0,11 h-1 to 1 h-1 were investigated. Two SC fermentations were conducted for the 20 g ℓ-1 glucose feed concentration and one for the 40 g ℓ-1. All SC fermentation runs were operated in excess of 12 days, while the biofilm run lasted 6,5 days. Fermentations were terminated only after contamination by lactic acid bacteria was observed. SC fermentations with the glucose feed concentration of 20 g ℓ-1 achieved a maximum SA ...
Infection may be inapparent, but 15-30% of pigs may suffer depression, inappetence, high fever (41.5°C, 107°F), or laboured breathing, especially after rising to drink of after disturbance within hours of infection. Cyanosis (a purple tinge of the ears and feet), subnormal rectal temperature and death may follow within 4-6 hours of the onset of clinical signs.. Blood stained froth may be seen at the mouth and such pigs will die. Mortality may reach 30-50% of affected pigs. Less severely affected pigs do not eat, are fevered, and show respiratory distress, coughing and exercise intolerance and do not pull away strongly when restrained. Some of these pigs may also die. Recovering animals cough, and show respiratory distress, particularly when disturbed. Exercise intolerance may continue for days and affected animals may eat less, appear gaunt and hairy, are depressed and show reduced rates of live weight gain.. Non-immune animals of all ages may be affected and abortion may occur in affected ...
What causes rheumatoid arthritis?. The search, by scientists around the world, has been exhaustive, and the prey surprisingly elusive - but in breakthrough Johns Hopkins research, scientists Maximilian Konig, M.D., and Felipe Andrade, M.D., Ph.D., believe they have pinpointed one culprit: a kind of bacteria that lives in the mouth and is present in gum disease.. This form of bacteria has an unpronounceable name, Aggregatibacter actinomycetemcomitans, so the scientists who have discovered the terrible and important role it might play in RA just call it by its initials: Aa. "We are very excited about it," says Konig. If their hypothesis is verified by other scientists and in larger studies, the possibilities of preventing RA from developing, and of treating early RA and possibly reversing symptoms, are enormous. This work was presented at the Plenary Session of the American College of Rheumatologys Annual Scientific Meeting in November 2016, and I recently wrote about it for Leap magazine, a ...
The assumption that USS arise by mutation rather than copying was first confirmed using alignments of H. influenzae proteins with distant homologues. Abundant USS-like repeats were found in all eight Pasteurellacean genomes; the repeat consensuses of species in the Hin subclade were identical to that of H. influenzae (AAGTGCGGT), whereas members of the Apl subclade shared the consensus ACAAGCGGT. All species USSs had the strong consensus and flanking AT-rich repeats of H. influenzae USSs. DNA uptake and competition experiments demonstrated that the Apl-type repeat is a true USS distinct from the Hin-type USS: A. pleuropneumoniae preferentially takes up DNA fragments containing the Apl-type USS over both H. influenzae and unrelated DNAs, and H. influenzae prefers its own USS over the Apl type ...
In many countries, pig health and pig welfare have been important topics, but pig producers themselves tend to get less attention. Nevertheless, respiratory problems among swine producers appear to be a fairly common phenomenon - and they can strike worldwide. There are ways to prevent these.
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We have observed that autoantibodies reactive with important phospholipid antigens are present in the sera and gingival crevicular fluids of patients with periodontitis. The antibodies are likely induced by commonly occuring plaque bacteria. Phosphorylcholine (PC) is a prevalent antigen found on 30-40% of plaque bacteria, and patients with periodontal attachment loss have significantly higher concentrations of IgG anti-PC than do individuals without attachment loss. Data indicate that these antibodies bind to and opsonize oral microorganisms such as Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) and further that they react with and opsonize modified low-density lipoproteins (LDL). Anti-β2-glycoprotein 1-dependent anti-cardiolipin (anti-CL), another antibody of interest, is found at elevated levels in 15-20% of patients with generalized periodontitis. In patients with systemic lupus erythemathosis (SLE) or the antiphospholipid syndrome (APS) elevated levels of these antibodies ...
Abstract Contagious bovine pleuropneumonia (CBPP) is an economically important disease in most of sub-Saharan Africa. A conjoint analysis and ordered probit regression models were used to measure the preferences of farmers for CBPP vaccine and vaccination attributes. This was with regard to inclusion or not of an indicator in the vaccine, vaccine safety, vaccine stability as well as frequency of vaccination, vaccine administration and the nature of vaccination. The analysis was carried out in 190 households in Narok District of Kenya between October and December 2006 using structured questionnaires, 16 attribute profiles and a five-point Likert scale. The factors affecting attribute valuation were shown through a two-way location interaction model. The study also demonstrated the relative importance (RI) of attributes and the compensation value of attribute levels. The attribute coefficient estimates showed that farmers prefer a vaccine that has an indicator, is 100% safe and is administered by ...
The study was conducted in two selected districts of Southern Omo zones of Ethiopia, namely Hammer and Benna-Tsemay, during November 2004 and May 2005 to determine the status of contagious caprine pleuropneumonia (CCPP). Participatory disease investigation was conducted in the goat flocks owned by pastoralists of the districts. Participatory methods such as proportionate piling and matrix scoring of diseases were used to characterise major diseases of goats. Clinical and post-mortem examinations and isolation of the causative agent of CCPP were done. Serological tests were conducted using CFT. CCPP (locally termed Sompo) ranked as the first important disease of goats in the study area. Local perception of causes and signs of CCPP were described. Matrix scoring between groups revealed that disease signs and causes showed weak, moderate and good agreement by Kendalls coefficient concordance (W = 0.21-0.99). The overall sero-prevalence of CCPP was 15.5%. The causative agent was isolated from sick ...
This is caused by a variety of bacteria, that gain access to the middle part of the ear which is responsible for balance and infection causes the piglet to hold its head on the affected side and to lose its balance.. Infection probably arises from the tonsils at the back of the throat and travels down the eustachian tube to the middle part of the ear. The common organisms involved include; Haemophilus parasuis, streptococci and staphylococci. The condition is sporadic but common particularly in the weaned pig and sow and occurs occasionally in the sucking pig from 7 to 10 days of age. In some farms up to five percent of weaner pigs may be affected. If treatment is prompt there is usually a good response. If treatment is delayed there is the risk that infection will spread from the middle ear into the inner ear and directly to the brain, setting up a meningitis or encephalitis (inflammation of the brain). Actinobacillus pleuropneumonia has been identified in outbreaks of the disease.. ...
In the swine industry today, there are four diseases that no swine producer should live with - swine dysentery, atrophic rhinitis, sarcoptic mange and Actinobacillus pleuropneumonia (APP). If present, consider eradication vs. control and stabilization protocols. Each one of these has been successfully removed from a majority of swine farms in North America, so these will not be addressed further in this article
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This study was carried out in Njiru and Dagorreti slaughterhouses with the objective of comparing the results of complement fixation test (CFT) and pathological lesions in lung samples taken for culture and sero-prevalence of Contagious Bovine Pleuropneumonia (CBPP) among cattle brought for slaughter. A total of 804 serum samples and 40 suspected lung tissues were collected from both abattoirs for laboratory diagnosis. The serum and lung tissues were collected from adult cattle that were brought for slaughter. 416 serum samples were taken from Njiru and the other 388 from Dagoretti slaughterhouse.The serum samples were subjected to the complement fixation test and the lung tissues cultured in both solid media and broth. Growth of the microorganisms in the media was checked once a week for a period of one month. Any media that had no growth after a period of one month was recorded as negative. Animals for slaughter originated from 22 districts and the majority were from the arid and semi-arid ...
Blood samples collected from 945 cattle at four local abattoirs in Turkey were examined for contagious bovine pleuropneumonia (CBPP) by the complement fixation test (cFT) and competitive ELISA (cELISA). In addition, the carcases of the animals were examined macroscopically at the abattoirs and 62 lung samples which had lesions suggestive of CBPP were collected for bacteriological culture. To identify suspicious isolates the PCR was used in addition to the routine biochemical tests. By the cFT, two of the 945 serum samples were seropositive, and by the cELISA, four of them were seropositive. In the bacteriological culture of the lungs, growth was observed in 18 (29 per cent) of the samples by the observation of turbidity in the broths. However, when these broths were inoculated into an agar base, growth was observed in only three (4-8 per cent) samples. These isolates were identified as Mycoplasma species on the basis of biochemical tests. In the PCR analysis of DNA extraded from the broths, none ...
Contagious bovine pleuropneumonia (CBPP) is a severe infectious disease caused by Mycoplasma mycoides subsp. mycoides small colony type (M. mycoides SC) and is a vast problem in Africa. Current CBPP prevention is based on attenuated live strain vaccines, but these are limited by factors such as short-term immunity, cold-chain dependence and retained virulence. CBPP can be diagnosed using post-mortem examination, identification of the agent using culture and PCR based methods as well as serological diagnostic methods, but the latter are generally not sensitive enough and there is also demand for an inexpensive, pen side field test.The research presented in this thesis was focused on using recombinantly expressed surface proteins from M. mycoides SC to characterize humoral immune responses to CBPP. Thereby candidate proteins to be used in development of serological diagnostic methods and possibly subunit vaccines could be identified. As a first step, five putative variable surface proteins of M. ...
Contagious Bovine Pleuropneumonia, formely under control, is once again a major threat to production across Africa, according to a report from the World Food and Agriculture Organisation.
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The right lung (1) has a normal pink colour and has collapsed as the chest was opened. The left lung (2) has not collapsed; it is firm, discoloured and fleshy. It is coated with a yellow fibrin deposits, which are also present on the inside of the ribs. Often parts of the lungs adhere to the chest wall ...
Harrison, Freya and Muruli, Aneesha and Higgins, Steven and Diggle, Stephen P. (2014) Development of an ex vivo porcine lung model for studying growth, virulence, and signaling of Pseudomonas aeruginosa. Infection and Immunity, 82 (8). pp. 3312-3323. ISSN 1098-5522 ...