Investigation of the structure of lipid A from Actinobacillus actinomycetemcomitans strain Y 4 and human clinical isolate PO 1021-7
TY - GEN. T1 - Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes. AU - Vlysidis, Anestis. AU - Du, Chenyu. AU - Webb, Colin. AU - Theodoropoulos, Constantinos. PY - 2008/12/1. Y1 - 2008/12/1. N2 - An unstructured kinetic model for the growth of Actinobacillus succinogenes on glycerol as the only carbon source is proposed in this study. The model describes cell growth considering both substrate and product inhibition. and its parameters are estimated by fitting the model predictions to experimental data. Substrate consumption and product formation rates are described by the Luedeking-Piret model. The main product of the process is succinic acid while by-products like acetate, formate and ethanol have very low concentrations. The main environmental factors that affect the bioprocess were examined and optimum conditions in terms of yield, final succinic acid concentration and productivity were evaluated by a factorial experimental ...
Succinic acid (SA) is poised to become a significant building-block or platform chemical in the bio-based economy. Of the microbial strains that show promise for biological SA production, the wild-type bacterium Actinobacillus succinogenes is one of the top-contenders. While strides have been made towards understanding the behaviour of the organism and developing the fundamentals for an industrial process based on the organism, there is still a large scope of research required and a multitude of challenges to be addressed. In particular, an improved understanding of the metabolism of the organism under favourable biofilm conditions is required and its potential as a microbial host in a biorefinery setting needs to be established. Therefore, the aim of this thesis is to develop a fundamental understanding of the central metabolism of the organism under biofilm conditions on relevant substrates, and to determine its performance as a SA producer on scalable biorefinery streams. Continuous operation ...
Looking for Actinobacillus capsulatus? Find out information about Actinobacillus capsulatus. A genus of gram-negative, immotile and nonspore-forming, oval to rod-shaped, often pleomorphic bacteria which occur as parasites or pathogens in mammals ,... Explanation of Actinobacillus capsulatus
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinobacillus hominis.
Actinobacillus equulis: especie que se encuentra en la cavidad bucal y amígdalas del caballo y causa lesiones supurativas de los riñones y articulaciones de los cerdos y caballos y endocarditis en el cerdo ...
University of California School of Veterinary Medicine, Department of Medicine and Epidemiology, Davis, CA 95616 Introduction and Objectives. Bacterial infections are the leading cause of death in foals during the first month of life. Ceftiofur, a 3rd-generation cephalosporin, shows excellent in vitro activity against many Gram-positive and Gram-negative bacteria, including E. coli, Actinobacillus spp, and other important pathogens of neonatal foals. This antimicrobial has recently been approved for treating Streptococcal respiratory tract infections in horses at a 24-hour dosage interval and at doses (2.2 to 4.4 mg/kg IM) substantially lower than those recommended for use of other 3rd-generation cephalosporins. The pharmacokinetics of ceftiofur administered IM to foals and adult horses have been reported, but no data is available concerning the pharmacokinetics of ceftiofur after IV use, the preferred administration route for critically ill foals.. The objectives of this study were:. 1. To ...
Aggregatibacter (Actinobacillus) actinomycetemcomitans is a bacterium mainly associated with aggressive forms of periodontitis. Among its virulence factors, a leukotoxin is suggested to play an important role in the pathogenicity. Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. Leukotoxin selectively kills human leukocytes and can disrupt the local defense mechanisms. Previous studies examining the role of the leukotoxin in host-parasite interactions have mainly focused on polymorphonuclear leukocytes (PMNs). In the inflamed periodontium, macrophages play a significant role in the regulation of the inflammatory reactions and the tissue breakdown and remodeling.. Thus, the aim of this dissertation was to investigate death mechanisms of human macrophages exposed to leukotoxin.. Human lymphocytes, PMNs, and monocytes/macrophages isolated from venous blood were exposed to purified leukotoxin or live A. actinomycetemcomitans ...
TY - JOUR. T1 - Infection by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, and antibody responses at different ages in humans.. AU - Nakagawa, S.. AU - Machida, Y.. AU - Nakagawa, Taneaki. AU - Fujii, H.. AU - Yamada, S.. AU - Takazoe, I.. AU - Okuda, K.. PY - 1994/1. Y1 - 1994/1. N2 - This study examined the serum IgG and IgM responses against Porphyromonas gingivalis and 3 serotypes of Actinobacillus actinomycetemcomitans, and the correlations of these responses with age and homologous infection. A total of 90 individuals were included in this study: 40 subjects with gingivitis, 40 periodontally healthy subjects, and 10 adult periodontitis subjects. The subjects in the gingivitis and periodontally healthy groups were divided into 4 stages based on their physiological age: early childhood, school age, puberty, and adult. In the gingivitis group, there was a positive correlation between increase in age and increase in serum IgG antibody levels against P. gingivalis until ...
Two previous studies have observed natural transformation in A. actinomycetemcomitans. Tøonjum et al. (30) used a broth-based method and identified two naturally competent strains among five study strains of A. actinomycetemcomitans with transformation frequencies of 1.3 × 10−3 and 6.2 × 10−5. In our present study, we identified a single highly competent A. actinomycetemcomitans strain among 17 strains examined. Although this is an apparently lower prevalence of naturally competent A. actinomycetemcomitans strains than was reported by Tøonjum et al., the transformation frequencies reported in this study were similar.. Sato et al. (23) generated transposon insertional mutants from the A. actinomycetemcomitans strain Y4 by natural transformation. The transformation was performed with broth-cultured exponential-phase A. actinomycetemcomitans cells. However, strain Y4 was not transformable in our study. While the loss of competence is sometimes a consequence of laboratory propagation, it is ...
0165] (1) Wilson M, Henderson, B. Virulence factors of A. actinomycetemcomitans revenant to the pathogenesis of inflammatory periodontal diseases. FEMS 1995:17:365-379. [0166] (2) Nowotny A, Behling U H, Hammond B, et al. Release of toxic microvesicles by Actinobacillus actinomycetemcomitans. Infect Immun 1982:37:151-154. [0167] (3) Kaplan J B, Perry M B, MacLean L L, Furgang D, Wilson M E, Fine D H. Structural and genetic analyses of 0 polysaccharide from Actinobacillus actinomycetemcomitans serotype f. Infect Immun 2001:69:5375-5384. [0168] (4) Rosan B, Slots J, Lamont R J, Listgarten M A, Nelson G M. Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol 1988:3:58-63. [0169] (5) Inouye T, Ohta H, Kokeguchi S, Fukui K, Kato K. Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans. FEMS microbiology letters 1990: 57: 13-17. [0170] (6) Kaplan J B, Schreiner H C, Furgang D, Fine D H. Population structure and genetic diversity of Actinobacillus ...
Structures of the antigenic O-polysaccharides of lipopolysaccharides produced by Actinobacillus actinomycetemcomitans serotypes a, c, d and e
A little over two years ago, we commented on a general rise in the frequency of Actinobacillus suis (A. suis) isolation from our swine tissue submission cases, and wondered if it was related to the increased porcine circovirus type 2 (PCV2) activity (National Hog Farmer North American Preview, June 6, 2008).
Actinobacillus suis has been isolated from the lungs of a 9-month-old cat. The bacterium was characterized biochemically as well as genetically, and its sensitivity profile to different antimicrobial agents was established. The role of this isolate in the cats condition is discussed ...
Many gram-positive and gram-negative bacterial species express LuxS and secrete and respond in various ways to a signal that is related to autoinducer 2 of V. harveyi (4, 7, 9, 10, 14, 16, 18, 19, 20, 21, 30, 31, 41). However, few of these organisms possess the dedicated two-component circuit that mediates the cell density-dependent response of V. harveyi to AI-2. Our previous studies showed that AI-2 secreted by the oral pathogen A. actinomycetemcomitans was capable of inducing V. harveyi bioluminescence (10) and also influenced aerobic growth of A. actinomycetemcomitans under iron-limiting conditions (9) These studies also suggested that AI-2-mediated regulation of iron uptake and storage genes contributed to optimal growth of A. actinomycetemcomitans under iron limitation. However, the mechanism by which A. actinomycetemcomitans responds to AI-2 is not fully understood. Analysis of the complete genome sequence of A. actinomycetemcomitans HK1651 (26; www.oralgen.lanl.gov ) indicates that this ...
A disease characterized by suppurative and granulomatous lesions in the respiratory tract, upper alimentary tract, skin, kidneys, joints, and other tissues. Actinobacillus lignieresii infects cattle and sheep while A. equuli infects horses and pigs ...
Wooden Tongue in Cattle Also known as: Actinobacillosis Wooden tongue (also known as Actinobacillosis) is a well-defined disease of the soft tissues of the mouth region in adult cattle. It is caused by Actinobacillus lignieresii, part of the normal bacterial flora of the upper digestive tract.
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Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
Abiotrophia balaenopterae Lawson et al. 1999, sp. nov. (Part 2) Abiotrophia elegans Roggenkamp et al. 1999, sp. nov. (Part 1) Acidovorax defluvii Schulze et al. 1999, sp. nov. (Part 4) Actinobacillus succinogenes Guettler et al. 1999, sp. nov. (Part 1) Actinobispora alaniniphila Xu et al. 1999, sp. nov. (Part 2) Actinobispora aurantiaca Xu et al. 1999, sp. nov. (Part 2) Actinobispora xinjiangensis Xu et al. 1999, sp. nov. (Part 2) Actinomyces bowdenii Pascual et al. 1999, sp. nov. (Part 4) Aerococcus christensenii Collins et al. 1999, sp. nov. (Part 3) Agrobacterium meteori Rüger and Höfle 1992 is assigned to be a synonym of Agrobacterium atlanticum Rüger and Höfle 1992. (Part 1) Agrococcus citreus Wieser et al. 1999, sp. nov. (Part 3) Ahrensia Uchino et al. 1999, gen. nov. (Part 1) Ahrensia kielensis corrig. (ex Ahrens 1968) Uchino et al. 1999, sp. nov., nom. rev. - Synonym: Agrobacterium kieliense Ahrens 1968. (Part 1) Alterococcus Shieh and Jean 1999, gen. nov. (Part 2) Alterococcus ...
Effective publication: POHL (S.): Reklassifizierung der gattung Actinobacillus Brumpt 1910, Haemophilus Winslow et al. 1971 und Pasteurella Trevisan 1887 anhand phänotypischer und molekularer daten, insbesondere der DNS-verwandtschaften bei DNS: DNS-hybridisierung in vitro und vorschlag einer neuen familie, Pasteurellaceae. Inaug. Diss. Phillips-Universität Marburg/Lahn. Mauersberger, Marburg, 1979 ...
Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL_1366 (APJL_1366) datasheet and description hight quality product and Backed by our Guarantee
Actinobacillus pleuropneumoniae ApaA protein: an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first source
Fifty randomly selected fattening pig herds were studied to investigate the epidemiological characteristics of infections with Actinobacillus pleuropneumoniae serovars 2, 3 and 9, and to identify risk factors for their within-herd seroprevalences. Information about 13 farm characteristics was obtained by means of a questionnaire and used to assess potential risk factors for the percentage of slaughter pigs with antibodies against each of the three serovars. The presence of antibodies was measured with an indirect ELISA. The median within-herd seroprevalence for serovar 2 was 58 per cent (range 0 to 100 per cent), for serovar 3, 53 per cent (range 10 to 95 per cent), and for serovar 9, 35 per cent (range 5 to 100 per cent). All but one farm tested positive for A pleuropneumoniae serovar 2, and all the farms were positive for A pleuropneumoniae serovars 3 and 9. There was a positive association (P<0.05) between each pair of serovars. The within-herd seroprevalence of serovar 2 was ...
0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium to which a calcium salt (i.e. a chemical compound, based on an acid, formed by replacing all or part of the hydrogen ions of the acid with one or more calcium ions) is added. In particular, EP 0 453 024 already describes such a method (see "Example 2", paragraph 2 "Purification and characterisation of hemolysin", subparagraph "Methods"). Note that ApxI used to be referred to as "HLY" (see Frey et al. in "J Gen Microbiol. 1993 August; 139(8): 1723-8"). From this EP patent it is known to add a calcium compound (CaCl2) to the medium. Indeed, in Microbiol Pathogenesis 37 (2004) 29-33 it is stated that transcriptional activity of the ApxI operon is enhanced by addition of calcium to the growth medium. This way, high levels of ApxI can be provided for. The medium should support the growth of APP bacteria. It is commonly known how to constitute a medium that supports growth of ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds from the central region of Chile. Arch. med. vet. [online]. 2008, vol.40, n.2, pp.147-153. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X2008000200006.. In Chile, there was only one existing study on App. This study was designed to determine the maternal immunity duration, the age of seroconversion and the apparent and true prevalence in animals from 7 swine commercial herds. 60 samples were taken per herd and divided into 10 serum samples from animals of 4, 6,10,14,18and21 weeks of age, which were analyzed by ELISA®. Out of the 420 samples, 134 were positive with 112 of them belonging to pigs under 10 weeks of age while only 22 were from animals over 10 weeks of age, which seroconverted presumably due to a natural infection. Regarding maternal immunity duration it was found that around the 10th week of age the animals lose their colostrum antibodies. In ...
Actinobacillus pleuropneumoniae ATCC ® 33590™ Designation: Femo TypeStrain=True Application: Characterization Respiratory research
Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). Following infection of 12 pigs, clinical signs of pneumonia developed within 20 h, whereafter the animals received a single dose of either danofloxacin (2.5 mg/kg) or tiamulin (10 mg/kg). To test the discriminative properties of the biomarkers, the dosage regimens were designed with an expected difference in therapeutic efficacy in favour of danofloxacin. Accordingly, the danofloxacin-treated pigs recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin increased, whereas plasma zinc, ascorbic acid and alpha-tocopherol decreased. In the ...
Periodontitis is mankinds most common chronic inflammatory disease. One severe form of periodontitis is localized aggressive periodontitis (LAP), a condition to which individuals of African origin demonstrate an increased susceptibility. The main causative organism of this disease is Actinobacillus …
Aggregatibacter aphrophilus (formerly Haemophilus aphrophilus and H. paraphrophilus ) is part of the normal oropharyngeal flora. It is a
SUMMARY. A case of bilateral epididymitis in a young Friesland bull is described. Actino-bacillis seminis was isolated from the bulls semen. The organism proved to be identical in microscopical, cultural and biochemical characteristics to strains isolated from rams. Serum from this animal, when subjected to a complement fixation test, yielded a suspicious reaction against an antigen prepared from the isolate of a naturally infected ram with clinical epididymitis. Blood sera from two bulls in the Orange Free State gave positive and suspicious reactions respectively to the same antigen.. ...
ID ACTP7_1_PE100 STANDARD; PRT; 215 AA. AC ACTP7_1_PE100; B3GZU0; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Cytochrome c-type protein NrfB; (ACTP7_1.PE100). GN Name=nrfB; OrderedLocusNames=APP7_0100; OS ACTINOBACILLUS PLEUROPNEUMONIAE SEROVAR 7 STR. AP76. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Actinobacillus. OX NCBI_TaxID=537457; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ACTP7_1.PE100. CC Actinobacillus pleuropneumoniae serovar 7 str. AP76, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:B3GZU0_ACTP7 CC -!- GENE_FAMILY: HOG000278525 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; B3GZU0; -. DR EMBL; CP001091; ACE60752.1; -; Genomic_DNA. DR RefSeq; YP_001967894.1; NC_010939.1. DR ProteinModelPortal; B3GZU0; -. DR STRING; B3GZU0; -. DR GeneID; 6397435; -. DR GenomeReviews; CP001091_GR; APP7_0100. DR ...
Food animals in the United States are often exposed to antimicrobials to treat and prevent infectious disease or to promote growth. Many of these antimicrobials are identical to or closely resemble drugs used in humans. Precise figures for the quantity of antimicrobials used in animals are not publicly available in the United States, and estimates vary widely. Antimicrobial resistance has emerged in zoonotic enteropathogens (e.g., Salmonella spp., Campylobacter spp.), commensal bacteria (e.g., Escherichia coli, enterococci), and bacterial pathogens of animals (e.g., Pasteurella, Actinobacillus spp.), but the prevalence of resistance varies. Antimicrobial resistance emerges from the use of antimicrobials in animals and the subsequent transfer of resistance genes and bacteria among animals and animal products and the environment. To slow the development of resistance, some countries have restricted antimicrobial use in feed, and some groups advocate similar measures in the United States. ...
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3.0.CO;2-5. PMID 10710235. Dawson SJ, White LA (1992). "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin". J. Infect. 24 (3): 317-20. doi:10.1016/S0163-4453(05)80037-4. PMID 1602151. Angel TA, Faust CJ, Gonzales JC, et al. (1993). "Genetic mapping of the X-linked dominant mutations striated (Str) and bare patches (Bpa) to a 600-kb region of the mouse X chromosome: implications for mapping human disorders in Xq28". Mamm. Genome. 4 (3): 171-6. doi:10.1007/BF00352233. PMID 8439729. Levin ML, Chatterjee A, Pragliola A, et al. (1996). "A comparative transcription map of the murine bare patches (Bpa) and striated (Str) critical regions and human Xq28". Genome Res. 6 (6): 465-77. doi:10.1101/gr.6.6.465. PMID 8828036. Heiss NS, Rogner UC, Kioschis P, et al. (1996). "Transcription mapping in a 700-kb region around the DXS52 locus in Xq28: isolation of six novel transcripts and a novel ATPase isoform (hPMCA5)". Genome Res. 6 (6): 478-91. doi:10.1101/gr.6.6.478. PMID 8828037. Mallon AM, ...
SWISS-MODEL Repository entry for B3GZ69 (KTHY_ACTP7), Thymidylate kinase. Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Importance of Streptococci in the reproduction disorders OF THE MARE. NEW DATAS. The authors described the important intervention of Strepto coccus zooepidemicus, equi, equisimilis and uberis in the bacterial disorders of reproduction (fertility, endometritis) and causes of abortion. The principal bacterial pathogens causing disorders are : Streptococci, Escherichia coli, Klebsiella pneumoniae, Actinobacillus equuli, Salmonella typhimurium, Pseudomonas aeruginosa. The Streptococcal diseases are found in over 50 per cent of confirmed cases of bacterial disorders of reproduction. The bacterial contamination access to the vagina and uterus of the mares, often, during the breeding of foaling. The bacteriological controls of mares and stallions are necessary before breeding and the research of several bacterial organisms is obligatory ...
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Introduction. The incidence of infective endocarditis (IE) has increased over the past years as survival rates of patients with congenital heart disease improve.1 Main agents of IE are: (i) Viridans group Streptococci (S. milleri, S. mitior, S. salivarius, S. mutans and S. sanguis), mainly on patients with congenital heart disease; (ii) Staphylococcus aureus, generally associated with placement of central venous catheter and use of injecting drugs; (iii) Staphylococcus epidermidis, usually affecting patients following cardiac surgery and catheterized premature newborns.1 On the other hand, the HACEK group of bacteria (Haemophilus ssp, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens and Kingella kingae) is responsible for 3% of IE in adults.1-3 The objective of this study is to report a case of rare occurrence of IE caused by Haemophilus aphrophilus in a child. Report of case The patient was a 12-year-old boy with a 20-day history of fever accompanied by chills ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. It was first reported in 1957, and was formally declared to be the causative agent of porcine pleuropneumonia in 1964. It was reclassified in 1983 after DNA studies showed it was more closely related to A. lignieresii. A. pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It exhibits β-hemolysis activity, thus explaining its growth on chocolate or blood agar, but must be supplemented with NAD (V factor) to facilitate growth for one of its biological variants (biovar 1). As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. Depending on the biovar, the bacteria may or may not be positive for urease; both biovars are positive for porphyrin. A. pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in ...
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific PCR, based on the capsule locus, which can be used in a multiplex format with the organisms specific gene apxIV. The PCR test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.. ...
Abstract Introduction Aggregatibacter aphrophilus formerly Haemophilus aphrophilus and H. Other infections reported in the literature include brain abscess, bone and joint infections and endophthalmitis.. There are only two cases of empyema ever reported due to this organism. We report the isolation of A. Case presentation A year-old female patient of Caucasian origin presented with a three-week history of fever, shortness of breath and dry cough. She was found to have a pleural empyema so a chest drain was inserted and a sample of pus was sent to the microbiology laboratory. They were oxidase- and catalase-negative. It was susceptible to amoxicillin, levofloxacin and doxycycline.. Our patient was treated with intravenous amoxicillin with clavulanic acid and clarithromycin followed by oral doxycycline, but was re-admitted twice over the next three months with recurrent empyema and the same organism was isolated.. Each episode was managed with chest drainage and a six-week course of ...
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. Persistent infections caused by bacterial biofilms are recalcitrant to treat because of the particular drug resistance of biofilm-dwelling cells. TolC, a key component of multidrug efflux pumps, are responsible for multidrug resistance in many Gram-negative bacteria. In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2, caused a significant reduction in biofilm formation, as well as increased drug sensitivity of both planktonic and biofilm cells. The genetic-complementation of the tolC1 mutation restored the competent biofilm and drug resistance. Besides, biofilm formation was inhibited and drug sensitivity was increased by the addition of phenylalanine-arginine beta-naphthylamide (PAβN), a well-known efflux pump inhibitor (EPI), suggesting a role
Pleuropneumonia infectioasa a porcului (PIP), cauzata de Actinobacillus pleuropneumoniae (App), poate fi intalnita la porcii de toate varstele, inclusiv la mistreti, dar simptomele clinice cele mai frecvente sunt inregistrate la porcii in faza de finisare, in varsta de peste 12 saptamani. In formele clasice de boala animalele manifesta febra si simptome respiratorii, datorate leziunilor caracteristice de pneumonie hemoragico-necrotica sau pleuropneumonie fibrinoasa. Boala poate evolua supra-acut, fara semne clinice, animalele fiind gasite moarte, acut, mai ales in efectivele naive imunologic, cu pierderi prin mortalitate de 15-20%, sau sub-acut, cu mortalitate mai redusa dar variabila. In efectivele infectate endemic boala se cronicizeaza la animalele care au supravietuit, afectand starea lor de bine si producand o scadere a performantelor zootehnice. Porcii pot fi purtatori fara sa dezvolte vreun raspuns imun. Boala genereaza pierderi economice insemnate.. ...
The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally ...
A preliminary study has been performed for the valorization of citrus peel waste (CPW) through the biorefinery platform aiming to produce succinic acid. Following extraction of essential oils and pectin, different conditions of dilute acid hydrolysis were evaluated based on estimation of the sugars liberated and subsequent fermentation of hydrolyzates for production of succinic acid by Actinobacillus succinogenes. The most suitable pretreatment conditions involved 116 degrees C for 10 min using 5% (w/v) of dry raw material (drm). Thus, a total sugar (ts) yield of 0.21 g(ts) g(drm)(-1) and a succinic acid (sa) yield via microbial fermentations of 0.77 g(sa) g(tsc)(-1) was achieved, while the use of lower solid contents resulted in higher sugar yields. The residues from dilute acid hydrolysis were applied for subsequent enzyme hydrolysis using commercial enzymes and the most suitable combination of enzyme units included 30 IU cellulases and 25 BGL beta-glucosidases achieving a yield of 0.58 g(ts) ...
Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules for florfenicol for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, pioneer and generic formulations, administered intramuscularly to pigs at a dose rate of 15 mg/kg. Antibacterial potency was determined in vitro as minimum inhibitory concentration (MIC) and Mutant Prevention Concentration in broth and pig serum, for six isolates of each organism. For both organisms and for both serum and broth MICs, average concentration:MIC ratios over 48 h were similar and exceeded 2.5:1 and times greater than MIC exceeded 35 h. From in vitro time-kill curves, PK/PD modelling established serum breakpoint values for the index AUC24h/MIC for three levels of inhibition of growth, bacteriostasis and 3 and 4log10 reductions in bacterial count; means were 25.7, 40.2 and 47.0 h, respectively, for P. multocida and 24.6,
ID C9R3L9_AGGAD Unreviewed; 404 AA. AC C9R3L9; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 25-OCT-2017, entry version 39. DE SubName: Full=Molybdopterin molybdenumtransferase {ECO:0000313,EMBL:ACX82405.1}; GN OrderedLocusNames=D11S_1018 {ECO:0000313,EMBL:ACX82405.1}; OS Aggregatibacter actinomycetemcomitans serotype C (strain D11S-1) OS (Actinobacillus actinomycetemcomitans). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Aggregatibacter. OX NCBI_TaxID=668336 {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569}; RN [1] {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=D11S-1 {ECO:0000313,EMBL:ACX82405.1, RC ECO:0000313,Proteomes:UP000002569}; RX PubMed=19820097; DOI=10.1128/JB.01203-09; RA Chen C., Kittichotirat W., Si Y., Bumgarner R.; RT "Genome sequence of Aggregatibacter actinomycetemcomitans serotype c RT strain D11S-1."; ...