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Components: s2146 Acridine Orange Stock Solution s1656 Sorensens Phosphate Buffer pH 6.0 s2147 Calcium Chloride 0.1M Solution s100 Acetic Acid 1% Aqueous
For speedy, intra-operative pathological margin assessment to steer staged cancers excisions, multimodal confocal mosaic check picture wide surgical margins (approximately 1?cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is definitely to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, […]. ...
We do routinely use acridine orange using FACScan. A 20 min rinse with 10 times water-diluted Chlorox bleach is adequate to clean the instrument. Zbigniew Darzynkiewicz -----Original Message----- From: Karen Creswell [mailto:creswelk at georgetown.edu] Sent: Friday, June 04, 2004 1:17 PM To: cyto-inbox Subject: Acridine Orange Does anyone routinely run acridine orange through their FACScan/FACSort? I would like suggestions for cleaning the instrument afterwards. Thanks for your help, Karen -- Karen Creswell, Ph.D. Director, Flow Cytometry/Cell Sorting Shared Resource Lombardi Cancer Center Georgetown University Medical Center Research Bldg W304 Box 571469 Washington, DC 20057-1469 202-687-2233 creswelk at georgetown.edu ...
... ,Application: Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a concentration of 120 μM will detect purified DNA in gels with a sensitivity of 25-50 ng per band. It has also been used extensively for cell staining of,biological,biology supply,biology supplies,biology product
The adsorption of the metachromic dye acridine orange (AO) by Na-beidellite was investigated by visible spectroscopy. Different types of clay-AO association were identified from the appearance and location of absorption bands alpha or beta . The colloidal properties were determined from curves of the absorbance vs. the degree of saturation. Three regions were identified in the absorbance curve. In the first region beidellite is peptized with small amounts of AO and the dye penetrates into the interlayer space where it undergoes metachromasy due to pi interactions between the aromatic entity and the oxygen plane of the clay. With larger amounts of AO (second region), the clay flocculates due to the aggregation of the dye cations in the interparticle space of the flocs. In excess AO (third region), beidellite is gradually peptized, forming small tactoids with monomeric AO in the interlayer space and at the same time adsorbing dimeric and polymeric AO cationic species at the solid-liquid interface. ...
BioAssay record AID 693243 submitted by ChEMBL: Induction of apoptosis in human MGC803 cells assessed as pyknosis at 5 uM after 12 to 48 hrs by acridine orange/ethidium bromide staining.
Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts.
HeLa cells. Light micrograph (acridine orange stain) of a culture of HeLa cancer cells. The nuclei of the cells are stained yellow while the cytoplasm is orange. HeLa cells are the first human cell line established in 1952 to research cancer. They were obtained from the cervix of Henrietta Lacks (after whom the cells are named) from Baltimore, USA, in 1951, who died of cervical cancer eight months later. HeLa cells thrive unusually well in the laboratory and are used in research worldwide. Professor Anderson (1911-2006) was the first to use acridine orange staining to study cells, bacteria and viruses. - Stock Image G442/0059
Background: Mitochondrial permeabilization provokes cardiomyocyte death, and causes adverse left ventricular remodeling in cardiac-restricted TNF transgenic mice (MHCsTNF). Autophagy, a lysosomal degradative pathway, is essential for removal of ubiquitin-tagged damaged mitochondria. Our prior work suggests that TNF receptor associated factor-2 (TRAF2), a multifunctional protein with E3 ubiquitin ligase activity, facilitates removal of damaged mitochondria. The role of TRAF2 vis-à-vis PARKIN, another E3 ubiquitin ligase essential for mitophagy, is unknown.. Methods and Results: MHCsTNF hearts and TNF-treated neonatal rat cardiac myocytes (NRCMs) demonstrate upregulation of both TRAF2 and PARKIN, with increased evidence for mitochondrial autophagy and reduced mitochondrial mass. To evaluate the role of TRAF2 and PARKIN in autophagic removal of damaged mitochondria, we employed gain-of-function and loss-of-function approaches; and assessed mitochondrial mass with nonyl-acridine orange fluorescence ...
Stain F-actin in cell samples with F-actin Staining Kit - Orange Fluorescence - Cytopainter ab112126. For fluorescence microscopes.
A while ago, scientists at the National University of Singapore created transgenic, fluorescent zebra fish by injecting red, green, and orange fluorescence genes from a jellyfish into the fish eggs. They did this so the fish could help detect environmental pollutants.Now, you can actually have them for pets! They are marketed under the name GloFish, and look very cool under a black light. Link - Thanks moniA!Previously on Neatorama: Fluorescent pig, rabbit, butterfly, and frog...
The metal chelating compound Dp44mT is a di-2-pyridylketone thiosemicarbazone (DpT) which displays potent and selective anti-tumor activity. This compound is receiving translational attention but its mechanism is poorly understood. Here we report that Dp44mT targets lysosome integrity through copper binding. Studies using the lysosomotropic fluorochrome acridine orange established that the copper-Dp44mT complex (Cu[Dp44mT]) disrupted lysosomes. This targeting was confirmed with pepstatin A-BODIPY FL, which showed re-distribution of cathepsin D to the cytosol with ensuing cleavage of the pro-apoptotic BH3 protein Bid. Redox activity of Cu[Dp44mT] caused cellular depletion of glutathione and lysosomal damage was prevented by co-treatment with the glutathione precursor N-acetylcysteine. Copper binding was essential for the potent anti-tumor activity of Dp44mT, since co-incubation with non-toxic copper chelators markedly attenuated its cytotoxicity. Taken together, our studies show how the lysosomal ...
The majority of phosphatase (PO4ase) activity detected in fresh aerobic activated sludge from a municipal wastewater treatment plant was associated with suspended floc material. PO4ase activity appeared to be localized in discrete bacteria-containing areas of the floc matrix based on the distribution of nucleic acid-stained cells and precipitated fluorescent crystals produced as a result of reaction of the enzyme(s) with the artificial substrate ELF™-PO4. Of the total floc-associated bacterial cells that stained positive with the nucleic acid-binding fluorochrome acridine orange (AO), 8.8 ± 1.2% displayed PO4ase activity based on the proximity of AO-stained cells to precipitated ELF crystals. Using a 16S rRNA oligonucleotide probe specific for the cytophaga-flavobacteria group, it was determined that 17-20% of the floc-associated bacteria that probed positive also displayed PO4ase activity. Furthermore, 35-45% of the ELF fluorescence was associated with bacterial cells that probed positive ...
Aims: In view of recent findings that a multidrug efflux pump CmeABC exists in Campylobacter jejuni, 391 C. jejuni and 52 Campylobacter coli of human and animal origin were examined for a multidrug resistance phenotype.. Materials and methods: The MICs of ampicillin, chloramphenicol, ciprofloxacin, erythromycin, kanamycin, tetracycline, cetrimide, triclosan, acridine orange, paraquat and ethidium bromide were determined. Resistance to organic solvents and the effect of salicylate (known inducer of the marRAB operon in Escherichia coli and Salmonella) were also examined.. Results: Two C. coli and 13 C. jejuni isolates, mainly from pigs or poultry, were resistant to three or more antibiotics and 12 of these strains had reduced susceptibility to acridine orange and/or ethidium bromide. Strains (n = 20) that were less susceptible to acridine orange, ethidium bromide and triclosan were significantly more resistant (P , 0.05) to ampicillin, chloramphenicol, ciprofloxacin, erythromycin, nalidixic acid ...
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The accuracy of techniques for the diagnosis of malaria are usually compared with optical microscopy, which is considered to be a gold standard. However, microscopy is prone to error and therefore makes it difficult to assess the reliability of other diagnostic techniques. We did a systematic review to assess the specificity and sensitivity of diagnostic techniques in different settings, using a statistical method that avoided defining a gold standard. Performance varied depending on species of the malaria parasite, level of parasitaemia, and immunity. Overall, histidine-rich protein 2 (HRP2)-based dipsticks showed a high sensitivity (92.7%) and specificity (99.2%) for Plasmodium falciparum in endemic areas. The acridine orange test was more sensitive (97.1%) in detecting P falciparum in epidemiological studies, with a specificity of 97.9%. In the absence of a gold standard, HRP2 dipsticks and acridine orange could provide an alternative for detecting falciparum infections in endemic areas and
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PromoKine offers a wide range of dyes for fluorescently staining dead cells. The membrane-impermeable nuclear stains can also be used for fixed cells. Commonly used dyes for staining dead cells include; orange-fluorescent ethidium bromide (EB), red-fluorescent propidium iodide (PI), green-fluorescent acridine orange (AO) and blue-fluorescent DAPI. The toluidine derivative Trypan Blue is a classical non-fluorescent dye used to selectively stain dead cells and shows a distinctive blue colour under the microscope. ...
A quiescent [denoted as Q(G0/G1)] subpopulation was isolated from EMT6/Ro-fed plateau monolayers by centrifugal elutriation. The median Coulter volume of these cells was significantly smaller than that of the original population from which they were elutriated. Using two-step acridine orange staining and dual parameter flow cytometric analysis, over 95% of quiescent cells were found to have G1 DNA content, and 80% of the cells had a decreased RNA content as compared to rapidly proliferating exponential G1 cells. After labeling for 24 hr (two doubling times) with [3H]thymidine, less than 2% of the quiescent cells incorporated [3H]thymidine as measured by autoradiography. The colony-forming efficiency of these cells was not significantly different from that of exponential cells. When such Q(G0/G1) cells were replated in fresh medium at a lower density, there was a lag time of 30 hr before any increase in cell number was detected, after which the cell-doubling rate matched that of exponential ...
contact Luis Benitez at Oncological Research Unit, kAP 107-014, UNAM, Hosp. 21 Sieglo, Mexico DF 06700, Mexico. He has some interesting results along this line. In article ,905kf544 at invrof.anmat.sld.ar, elisa at invrof.anmat.sld.ar (Dra. Elisa Bal de Kier-Joffe) writes: ,Dear netters: ,We are using Acridine Orange (AO) to stain apoptotic cells. , ,The nuclei staining of apoptotic cells is very easily recognized when it is ,in an advanced stage. ,However when the process is early I observe only a brighter staining of ,chromatin clumps, and a more marked limit in nuclei periphery. When this ,happens, the cytoplasm shows a diffuse red staining which is also evident in ,definite apoptotic cells. , ,In contrast normal alive cells only show a pale diffuse green staining of ,the nuclei and cytoplasm and only a kind of red granule (RNA) in the ,cytoplasm next to the nucleus. , ,I have not found any comments concerning this early diffuse intense red ,staining of the cytoplasm with AO in apoptotic ...
A novel method for the detection, screening, the quantitation of compounds that interact with nucleic acids (DNA or RNA) is provided. The basis for this method is the inhibition of acridine orange binding to the nucleic acid. Measurement of binding inhibition utilizes the rapid, repeatable technique of fluorescence polarization.
Fluorescent micrographs and quantitative analysis of CAOV-3 cells double-stained with acridine orange and propidium iodide. Cells were treated with liriodenine
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17784-47-3 - XUESTGHCVFYOLL-UHFFFAOYSA-N - Acridine, hydrochloride - Similar structures search, synonyms, formulas, resource links, and other chemical information.
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Deep, Dark Salted Butter Caramel Sauce, for serving (after using Zoe Nathans, decided that I definitely prefer my own, reprinted below). 1. In a food processor, pulse the 1 cup of flour with 2 tablespoons of the sugar and the baking powder and salt. Add the stick of cold butter and pulse several times, just until it is the size of peas. Sprinkle the dough with the ice water and pulse just until moistened crumbs form. Turn the crumbs out onto a work surface, knead once or twice and pat the pastry into a disk. Wrap the pastry in plastic and chill for 30 minutes.. 2. On a floured work surface, roll out the pastry to an 11-inch round, about 1/4 inch thick. Transfer the pastry to a parchment paper-lined flat cookie sheet and refrigerate for 15 minutes, or until chilled.. 3. Meanwhile, peel the blood oranges, removing all of the bitter white pith. Thinly slice 2 of the oranges crosswise; remove the pits. Transfer the orange slices to a plate. Working over a sieve set over a bowl, cut in between the ...
Deep, Dark Salted Butter Caramel Sauce, for serving (after using Zoe Nathans, decided that I definitely prefer my own, reprinted below). 1. In a food processor, pulse the 1 cup of flour with 2 tablespoons of the sugar and the baking powder and salt. Add the stick of cold butter and pulse several times, just until it is the size of peas. Sprinkle the dough with the ice water and pulse just until moistened crumbs form. Turn the crumbs out onto a work surface, knead once or twice and pat the pastry into a disk. Wrap the pastry in plastic and chill for 30 minutes.. 2. On a floured work surface, roll out the pastry to an 11-inch round, about 1/4 inch thick. Transfer the pastry to a parchment paper-lined flat cookie sheet and refrigerate for 15 minutes, or until chilled.. 3. Meanwhile, peel the blood oranges, removing all of the bitter white pith. Thinly slice 2 of the oranges crosswise; remove the pits. Transfer the orange slices to a plate. Working over a sieve set over a bowl, cut in between the ...
Pill with imprint SHIRE 381 30mg is Orange / Orange, Capsule-shape and has been identified as Adderall XR 30 mg. It is supplied by Shire US Inc..
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Youre in the right place if you want to avoid the orange, and who doesnt. Lets begin by explaining how skin can turn orange. Over-application (that can be too much in one application and/or applying too frequently), using budget brands that use lower-quality ingredients, and applying when your skin is noticeably dry. Follow these tips to lessen the dreaded orange:. ...
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What does God want for your life? What is Gods plan for you? Want some orange drink? These were the questions people asked me on Sundays when I was growing up as a teenager in church. We wondered about what God wanted for us, and wondered how we all could hold ourselves and our friends accountable for our behavior to keep us walking in the path of holiness. All, of course, discussed over the ubiquitous orange drink served at youth meetings across the country. Orange drink is the most righteous of bulk beverages.
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Dry Soda introduced its new Blood Orange flavor of less sweet soft drinks. Blood Orange Dry Soda has fruity, bright and high acidic characteristics and 50 calories per 12-ounce bottle.
Protein content and RDA percentage, per serving and per 100g, in 26 types of an orange. The amount of Protein is 3.6 g to 0 g per 100g, in an orange.
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