The present study aimed to perform a deep phenotypic and genotypic analysis of 15 clinical carbapenem-resistant Acinetobacter baumannii (CRAb) strains isolated in Madagascar between 2008 and 2016 from diverse sources. CRAb isolates collected from the Clinical Biology Centre of the Institut Pasteur of Madagascar, from the neonatal unit of Antananarivo military hospital, and from intensive care units of Mahajanga Androva and Antananarivo Joseph Ravoahangy Andrianavalona (HJRA) hospitals were subjected to susceptibility testing. Whole-genome sequencing allowed us to assess the presence of antibiotic-resistance determinants, insertion sequences, integrons, genomic islands and potential virulence factors in all strains. The structure of the carO porin gene and deduced protein (CarO) were also assessed in CRAb isolates. All isolates were found to be multidrug-resistant strains. Antibiotic-resistance genes against six classes of antimicrobial agents were described. The four carbapenem-resistance genes: blaOXA
This study reports the dissemination of multidrug-resistant (MDR) OXA-23-producing Acinetobacter baumannii clones in hospitals in Antananarivo, Madagascar. A total of 53 carbapenem-resistant A. baumannii isolates were obtained from September 2006 to March 2009 in five hospitals. These resistant strains represent 44% of all A. baumannii isolates. The double disk synergy test was performed to screen for production of metallo-beta-lactamases. Polymerase chain reaction (PCR) and DNA sequencing were performed for the detection of bla(AmpC), bla(OXA-51),bla(OXA-23), bla(OXA-24), bla(IMP), bla(VIM). The presence of the insertion sequence ISAba1 relative to bla OXA-23 and bla OXA-51 was assessed by PCR. Isolates were typed by Rep-PCR. All the isolates were MDR and produced the OXA-23 carbapenemase, which was confirmed by sequencing. PCR analysis for AmpC and OXA-51 gave positive results for all strains studied. No isolates produced metallo-beta-lactamases. In all isolates ISAba1 laid upstream of bla OXA-23. The
This study reports the dissemination of multidrug-resistant (MDR) OXA-23-producing Acinetobacter baumannii clones in hospitals in Antananarivo, Madagascar. A total of 53 carbapenem-resistant A. baumannii isolates were obtained from September 2006 to March 2009 in five hospitals. These resistant strains represent 44% of all A. baumannii isolates. The double disk synergy test was performed to screen for production of metallo-beta-lactamases. Polymerase chain reaction (PCR) and DNA sequencing were performed for the detection of bla(AmpC), bla(OXA-51),bla(OXA-23), bla(OXA-24), bla(IMP), bla(VIM). The presence of the insertion sequence ISAba1 relative to bla OXA-23 and bla OXA-51 was assessed by PCR. Isolates were typed by Rep-PCR. All the isolates were MDR and produced the OXA-23 carbapenemase, which was confirmed by sequencing. PCR analysis for AmpC and OXA-51 gave positive results for all strains studied. No isolates produced metallo-beta-lactamases. In all isolates ISAba1 laid upstream of bla OXA-23. The
The antibiotic susceptibility of Acinetobacter calcoaceticus-Acinetobacter baumannii complex strains recovered from the intensive care unit (ICU) of West China Hospital, Sichuan, PR China, from 2006 to 2009 was investigated. The identification of A. baumannii and analysis of carbapenemase-encoding genes and their relationship with ISAba1 were performed by PCR. Furthermore, a DiversiLab repetitive extragenic palindromic sequence-based PCR (rep-PCR) microbial typing system and a multilocus sequence typing (MLST) scheme were applied to assess the genetic relationship of the isolates. The results showed that the antibiotic susceptibility of the A. calcoaceticus-A. baumannii complex isolates changed and imipenem resistance increased rapidly between 2006 and 2009. The blaOXA-51-like and ISAba1-associated blaOXA-23 genes were prevalent in the imipenem-resistant A. baumannii isolates. However, the blaOXA-58-like gene was found in only one isolate and no metallo-β-lactamase genes were detected. The ...
Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon. Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis. Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D
Volume 48, no. 1, p. 224-228, 2004. Page 224, column 2, line 16: risk of IRAB infection should read risk of IRAB occurrence.. Page 225, column 2, line 49: IRAB should read imipenem-resistant Pseudomonas aeruginosa.. Page 225, column 2, line 51: ISAB should read imipenem-susceptible P. aeruginosa.. ...
Journal Article: Small-Molecule Transport by CarO, an Abundant Eight-Stranded beta-Barrel Outer Membrane Protein from Acinetobacter Baumannii ...
Objective: Carbapenem-resistant Acinetobacter baumannii (CR-AB) is an emerging cause of nosocomial infections worldwide. Combination therapy may be the only viable option until new antibiotics become available. The objective of this study is to identify potential antimicrobial combinations against CR-AB isolated from our local hospitals. Methods: AB isolates from all public hospitals in Singapore were systematically collected between 2006 and 2007. MICs were determined according to CLSI guidelines. All CR-AB isolates were genotyped using a PCR-based method. Clonal relationship was elucidated. Time-kill studies (TKS) were conducted with polymyxin B, rifampicin and tigecycline alone and in combination using clinically relevant (achievable) unbound concentrations. Results: 31 CR AB isolates were identified. They are multidrug-resistant, but are susceptible to polymyxin B. From clonal typing, 8 clonal groups were identified and 11 isolates exhibited clonal diversity. In single TKS, polymyxin B, ...
OmpA Binding Mediates the Effect of Antimicrobial Peptide LL-37 on Acinetobacter baumannii. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Background Kentucky Department for Public Health (KDPH) notified of Acinetobacter outbreak in September, 2010 Kentucky Department for Public Health (KDPH) notified of Acinetobacter outbreak in September, cases initially reported 66 cases initially reported Assistance requested from KDPH Assistance requested from KDPH New Infection Preventionist New Infection Preventionist Reported gaps in infection control process Reported gaps in infection control process Hand hygiene Hand hygiene Lacked comprehensive environmental cleaning protocols Lacked comprehensive environmental cleaning protocols 3
Acinetobacter baumannii has been associated with high morbidity and mortality rates, even in pediatric patients. Therapeutic options are limited, especially when the strain is multidrug resistant. Clinical and microbiological analyses of 4 cases of systemic infections caused by multi drug resistant A. baumannii treated with colistin/vancomycin combination at a Pediatric Intensive Care Unit were performed in order to explore the potential synergistic activity of colistin plus vancomycin. All the patients were treated with colistin, meropenem and vancomycin. Four severe infections due to MDR A. baumannii were observed. All patients treated with colistin/vancomycin combination had a positive outcome with no infection relapses. Most importantly, no significant adverse events related to the simultaneous administration of COL plus VAN were observed. In our in-vitro experiments, the synergistic effect of the combination COL plus VAN showed an early bactericidal activity even at VAN concentration of 16 mg/L,
Clinical features and outcomes of Acinetobacter species and Pseudomonas aeruginosa. Presented at the 46th Interscience infections due buy Xanax tablets online UK multidrug resistant baumannii Ab bloodstream infections BSI. Antimicrob Agents Chemother 200751376 378 391 Fournier PE. Clin Infect Dis 1996221026 1032 Carey RB, Banerjee SN. baumannii correlated with an increased 1999 Marmara earthquake. Surveillance cultures and duration of carriage of multidrug resistant Acinetobacter. Seasonal variation of Acinetobacter infections Clin Microbiol Rev 200619257. J Clin Microbiol 2006443623 3627 of resistance to tigecycline has. Crit Care Med 2005331136 1140 of broad spectrum antibiotics. References Schreckenberger PC, Daneshvar with multidrug resistant Pseudomonas aeruginosa. Presented at the 46th Interscience outbreaks of acinetobacter infections, most even more limited. PLoS Genet 20062e7 e7 of Acinetobacter spp. Source Information From Medical for nonsusceptibility in Acinetobacter baumannii. 79 An ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
TY - JOUR. T1 - Acinetobacter species as model microorganisms in environmental microbiology. T2 - current state and perspectives. AU - Jung, Jaejoon. AU - Park, Woojun. PY - 2015/3/1. Y1 - 2015/3/1. N2 - Acinetobacter occupies an important position in nature because of its ubiquitous presence in diverse environments such as soils, fresh water, oceans, sediments, and contaminated sites. Versatile metabolic characteristics allow species of this genus to catabolize a wide range of natural compounds, implying active participation in the nutrient cycle in the ecosystem. On the other hand, multi-drug-resistant Acinetobacter baumannii causing nosocomial infections with high mortality has been raising serious concerns in medicine. Due to the ecological and clinical importance of the genus, Acinetobacter was proposed as a model microorganism for environmental microbiological studies, pathogenicity tests, and industrial production of chemicals. For these reasons, Acinetobacter has attracted significant ...
outbreaks of carbapenem-resistant A. baumannii infection occurred in a hospital in New York City. Subsequently, numerous other hospitals in the United States and South America have had outbreaks of carbapenem-resistant A. baumannii. The incidence of infections with A. baumannii among military personnel from the United States and Canada has increased since 2002; 102 patients had bloodstream infections at facilities treating U.S. military personnel injured in Iraq or Afghanistan from January 1, 2002, through August 31, 2004. An epidemiologic investigation revealed that A. baumannii could be grown from environmental sites in field hospitals and that the environmental strains were closely related genotypically to clinical isolates. A. baumannii strains from injured military personnel from the United States and the United Kingdom were also genotypically related; this finding provided further evidence that A. baumannii was being acquired in field hospitals.. ...
War wound infection and osteomyelitis caused by multidrug-resistant (MDR) Acinetobacter species have been prevalent during the 2003-2005 military operations in Iraq. Twenty-three soldiers wounded in Iraq and subsequently admitted to our facility from March 2003 to May 2004 had wound cultures positive for Acinetobacter calcoaceticus-baumannii complex. Eighteen had osteomyelitis, 2 burn infection, and 3 deep wound infection. Primary therapy for these infections was directed antimicrobial agents for an average of 6 weeks. All soldiers initially improved, regardless of the specific type of therapy. Patients were followed up to 23 months after completing therapy, and none had recurrent infection with Acinetobacter species. Despite the drug resistance that infecting organisms demonstrated in this series, a regimen of carefully selected extended antimicrobial-drug therapy appears effective for osteomyelitis caused by MDR Acinetobacter spp.
REQUIMTE- Microbiology Research Group Goals Goals Epidemiology study of antibiotic resistant bacteria in order to implement containment measures, methodologies for their detection, and design of new compounds Hospitals Food and animal production environment Humans community Environment VRE, Enterobacteriaceae- PL/TEM-52; PL/CTX-M-15, Pseudomonas-VIM Enterococcus gentaR, Tn1546 PL/CTX-M-14; PL/CTX-M-15 (Enterobacteriaceae) VRE-CC17 Acinetobacter OXA-23 Salmonella-intI1 OXA-30, sul3 Enterobacteriaceae- PL/TEM-52 Salmonella-intI1MDR, Enterobacteriaceae- PL/TEM-52 Strategies Characterization of bacteria, genes and mobile genetic elements from different ecological niches AAC :1001 JAC (In press) AAC :1545 CMI :1131 JAC :297 CMI :1047 JAC :1139 EID :1985 AAC :836 AAC : AAC :451 JAC :1370 AAC :3613 AEM :3743 AAC :2140 AEM :3364 CMI :755 AAC :3220 Main Achievements (1) Emergence and International dissemination of MDR strains: VRE, ESBL-producing Enterobacteriaceae, Carbapenemase-producing Pseudomonas and
Infection with antibiotic-resistant Acinetobacter spp. is an increasing problem in critical care environments worldwide. Acinetobacter spp. are known to produce an insulin-cleaving protease. We hypothesized that infection with Acinetobacter spp. was associated with the acquisition of glucose intolerance in burn patients. Data were collected prospectively on all 473 patients admitted to the Burns Centre between January 2002 and March 2003. A total of 3.4% of patients acquired glucose intolerance during admission. Patients with Acinetobacter spp. infection were 9.8 times more likely to develop glucose intolerance than those without the infection (P | .0001). The association persisted after controlling for TBSA (P | .001). In patients with deep Acinetobacter spp. infection, 47% had glucose intolerance, compared with 12% in those with infection of the burn only (P = .03). In patients with pre-existing diabetes mellitus, 27% developed Acinetobacter spp. infection compared with only 8.5% of patients without
Introduction: Ventilator-associated pneumonia (VAP) caused by multidrug-resistant Acinetobacter baumannii (MDR-AB) is common in hospitals and impacts patient survival. We determined the incidence of MDR-AB VAP in critical care units and examined the predictors of 14-day mortality in these patients. Methodology: A retrospective case series study was conducted at a tertiary referral teaching hospital in north Jordan. A list of patients with a positive culture of A. baumannii between January 2007 and June 2013 was retrieved using computerized hospital databases. Medical records of all these patients were reviewed, and cases of VAP infected with MDR-AB were identified. Predictors of 14-day mortality were determined using multivariable logistic regression adjusted for possible confounders. Results: Out of 121 A. baumannii-VAP cases, 119 (98.3%) were caused by MDR-AB. The incidence rate of MDR-AB VAP was 1.59 cases per 100 critical care unit admissions. The mortality of A. baumannii-VAP cases in critical
title: In Vitro Interactions of Antibiotic Combinations of Colistin, Tigecycline, and Doripenem Against Extensively Drug-Resistant and Multidrug-Resistant Acinetobacter baumannii, doi: 10.3343/alm.2016.36.2.124, category: Article
Sayer refers to an example from the Korean Journal of Physiology and Pharmacology titled, Black raspberry root polyphenols exhibit antibacterial activity against drug resistant bacteria. He notes that the root-not the berry-of the black raspberry plant contains polyphenols which are lethal to methicillin-resistant Staphylococcus aureus (MRSA),carbapenem-resistant Acinetobacter baumannii (CRAB), and Bacillus anthracis (Anthrax). The roots contain these polyphenols because they dig into soil, a potentially hostile environment, while the berries dont have it because they serve as food for animals that help spread the black raspberry seed.. The fauna and flora of Gaia-our earth, our home-have learned over eons how to survive. Yet medicine presumes to know better. Antibiotics were first used in wars because the most common means of death in war isnt wounds, but the infections that develop from them. For this purpose, antibiotics worked brilliantly. But it must be noted that wholesale war is a ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. ...
The pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathway. One is irreversible oxidative phase in which glucose-6P is converted to ribulose-5P by oxidative decarboxylation, and NADPH is generated [MD:M00006]. The other is reversible non-oxidative phase in which phosphorylated sugars are interconverted to generate xylulose-5P, ribulose-5P, and ribose-5P [MD:M00007]. Phosphoribosyl pyrophosphate (PRPP) formed from ribose-5P [MD:M00005] is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway map also shows the Entner-Doudoroff pathway where 6-P-gluconate is dehydrated and then cleaved into pyruvate and glyceraldehyde-3P [MD:M00008 ...
tRNA-specific adenosine deaminase; Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2); Belongs to the cytidine and deoxycytidylate deaminase family (158 aa ...
Cytosine/purine/uracil/thiamine/allantoin permease family protein; Derived by automated computational analysis using gene prediction method- Protein Homology; Belongs to the purine-cytosine permease (2.A.39) family (468 aa ...
Rayani, Arezoo and Ahanjan, Mohammad and Goli, Hamid Reza and Naderifard, Niloofar and zamanzdeah, Maryam (2020) Comparing the Effect of Probiotic and Non-probiotic Yogurt Drinks on Two Common Oral Microorganisms: An In Vitro Study. Journal of Mazandaran University of Medical Sciences, 30 (185). pp. 33-40. Ranjbar, Termeh and Hashemi, zahra and Sadeghian, Fereshteh and Goli, Hamid Reza and Ahanjan, Mohammad and Ebrahimzadeh, Mohammad Ali (2020) Green Synthesis of Silver Nanoparticles with Allium paradoxum Extract and Evaluation of their Antibacterial Activities. Journal of Mazandaran University of Medical Sciences, 29 (182). pp. 1-11. NorouziBazgir, Zahra and Ahanjan, Mohammad and MohammadBagher, Hashemi Soteh and Goli, Hamid Rez (2019) Correction to: Prevalence of IMP and SPM Genes in Clinical Isolates of Carbapenem Resistant Acinetobacter baumannii in Educational Hospitals of Sari, Iran. Journal of Mazandaran University of Medical Sciences, 29 (176). pp. 227-228. NorouziBazgir, Zahra and ...
Bacterial infections due to Acinetobacter species are typically encountered in health care settings and can be particularly difficult to treat due to the propensity of the organism to incorporate multiple antibiotic resistance mechanisms. Hospital outbreaks of multidrug-resistant Acinetobacter infections have been reported. A wide range of infections are possible with this organism, including bloodstream infections, pneumonia (occasionally even community-acquired pneumonia), urinary tract infections, and wound infections (wound infection in soldiers after traumatic injury have been reported ...
TY - JOUR. T1 - A Novel Phenotypic Method To Screen for Plasmid-Mediated Colistin Resistance among Enterobacteriales. AU - Bell, Drew T.. AU - Bergman, Yehudit. AU - Kazmi, Abida Q.. AU - Lewis, Shawna. AU - Tamma, Pranita. AU - Simner, Patricia. PY - 2019/5/1. Y1 - 2019/5/1. N2 - Plasmid-mediated colistin resistance (PMCR), a consequence of the mcr genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity. Thus, we modified the colistin broth-disk elution (CBDE) test to screen for plasmid-mediated colistin resistance (PMCR) genes based on any reduction of colistin MIC in the presence of EDTA. Eighty-five isolates of the order Enterobacteriales (12 mcr positive) were tested by CBDE ± EDTA. The sensitivity and specificity of the EDTA-CBDE method to detect PMCR compared to the molecular genotype results were 100% and 95.8%, respectively. Isolates positive by the EDTA-CBDE test ...
TY - JOUR. T1 - Comparative genomic analysis of Acinetobacter oleivorans DR1 To determine strain-specific genomic regions and gentisate biodegradation. AU - Jung, Jaejoon. AU - Madsen, Eugene L.. AU - Jeon, Che Ok. AU - Park, Woojun. PY - 2011/10. Y1 - 2011/10. N2 - The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplete glucose metabolism, metabolic pathways of aromatic compounds, biofilm formation, antibiotics and metal resistance, and natural competence genes were conserved in four compared genomes. Interestingly, only strain DR1 possesses gentisate ...
Tigecycline is a last-resort antibiotic that is used to treat severe infections caused by extensively drug-resistant bacteria. tet(X) has been shown to encode a flavin-dependent monooxygenase that modifies tigecycline1,2. Here, we report two unique mobile tigecycline-resistance genes, tet(X3) and tet(X4), in numerous Enterobacteriaceae and Acinetobacter that were isolated from animals, meat for consumption and humans. Tet(X3) and Tet(X4) inactivate all tetracyclines, including tigecycline and the newly FDA-approved eravacycline and omadacycline. Both tet(X3) and tet(X4) increase (by 64-128-fold) the tigecycline minimal inhibitory concentration values for Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii. In addition, both Tet(X3) (A. baumannii) and Tet(X4) (E. coli) significantly compromise tigecycline in in vivo infection models. Both tet(X3) and tet(X4) are adjacent to insertion sequence ISVsa3 on their respective conjugative plasmids and confer a mild fitness cost (relative ...
Acrônimos com ACINETOBACTER. As definições de siglas Acinetobacter. As definições de acrónimo Acinetobacter. Sigla Acinetobacter significa para. Além de encontrar siglas. Encontre o que significam as siglas!
Newly published findings by a team of researchers in China describe the discovery of a plasmid-mediated mechanism in E. coli isolated from pigs that confers resistance to colistin - widely regarded as an antibiotic of last resort for treating drug resistant infections.. The researchers discovered the mechanism during a surveillance project looking at antibiotic resistance in E. coli from food animals when they found a large increase in the prevalence of colistin resistance in isolates. Further investigation revealed that a strain isolated from a pig, SHP45, possessed colistin resistance that could be transferred horizontally to another strain.. These findings led to the identification of a gene, mcr-1, conferring resistance to colistin and located on a plasmid within the bacterial cell. This is the first time that a resistance mechanism against polymyxin antibiotics like colistin has been found that is plasmid mediated and can be transferred horizontally to other bacteria.. Further studies ...
We characterized two new gene cassettes in an Acinetobacter isolate: one harbored the metallo-β-lactamase (IMP-4) gene blaIMP-4, the other harbored the rifampin ADP-ribosyltransferase (ARR-2) gene arr-2, and both arrayed with the aminoglycoside acetyltransferase [AAC(6′)-Ib7] gene cassette aacA4 in two separate class 1 integrons. The epidemiology of these gene cassettes in isolates from blood cultures obtained from 1997 to 2000 was studied. Isolates bearing either the blaIMP-4 or the arr-2 gene cassette or both represented 17.5% (10 of 57) of isolates in 1997, 16.1% (10 of 62) in 1998, 2.5% (1 of 40) in 1999, and 0% (0 of 58) in 2000. These two gene cassettes, probably borne on two separate integrons, were found in at least three genomic DNA groups, with evidence of clonal dissemination in the intensive care unit during 1997 to 1998. Seventeen of the 52 Acinetobacter baumannii (genomic DNA group 2) isolates from 1997 to 2000 harbored intI1, but only one was positive for these gene cassettes, ...
Carbapenems have traditionally been used to treat infections caused by Gram-negative bacteria resistant to many other agents, as they usually escaped the hydrolytic activity of most clinically relevant β-lactamases and are little affected (in terms of absolute MIC changes) by permeability and active efflux mechanisms. For years, resistance to these agents was mainly confined to non-fermentative Gram-negative bacteria resistant to ertapenem, and a few species (most notably Stenotrophomonas maltophilia) with intrinsic resistance to all carbapenems, because of a chromosomal encoded carbapenemase.. In 1991, a transferable carbapenemase was reported in Pseudomonas aeruginosa (strain GN17203).1 Shortly after, OXA-23 (ARI-1)2 in Acinetobacter baumannii and KPC-1 in Klebsiella pneumoniae3 were also described. In the following years, organisms producing plasmid-mediated carbapenemases have rapidly spread worldwide. A pooled analysis of nine studies comparing mortality in infections caused by ...
Aim: To determine the prevalence of New Delhi metallo-β-lactamase (NDM)-producing Gram-negative pathogens isolated from childrens samples. Materials & methods: Carbapenem-resistant clinical isolates (n = 117) were confirmed by VITEK® 2 compact system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing. MIC (μg/ml) of various antibiotics was determined by VITEK 2 compact system. Molecular characterization of the isolates was performed by PCR, DNA sequencing, PFGE and DNA hybridization. Results: Out of 117 carbapenemase producers, 37 (31.6%) and 29 (24.7%) were Klebsiella pneumoniae and Acinetobacter baumannii, respectively. 72 (61.5%) isolates were NDM positive and among these 60, 9 and 3 were NDM-1, -5 and -7, respectively. Majority of the NDM-producing K. pneumoniae belonged to ST11 and ST273 while most of the E. coli belonged to ST405 and ST101. blaNDM were mainly located on 150kb plasmids. MIC displayed high resistance against β-lactams drugs ...
海词词典,最权威的学习词典,专业出版acinetobacter winogradskyii是什么意思,acinetobacter winogradskyii的用法,acinetobacter winogradskyii翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
海词词典,最权威的学习词典,专业出版acinetobacter baummanii是什么意思,acinetobacter baummanii的用法,acinetobacter baummanii翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
Abstract: Chlorhexidine is widely used as an antiseptic or disinfectant in both hospital and community settings. A number of bacterial species display resistance to this membrane-active biocide. We examined the transcriptomic response of a representative nosocomial human pathogen, Acinetobacter baumannii, to chlorhexidine to identify the primary chlorhexidine resistance elements. The most highly up-regulated genes encoded components of a major multidrug efflux system, AdeAB. The next most highly overexpressed gene under chlorhexidine stress was annotated as encoding a hypothetical protein, named here as AceI. Orthologs of the aceI gene are conserved within the genomes of a broad range of proteobacterial species. Expression of aceI or its orthologs from several other γ- or β-proteobacterial species in Escherichia coli resulted in significant increases in resistance to chlorhexidine. Additionally, disruption of the aceI ortholog in Acinetobacter baylyi rendered it more susceptible to ...
Abstract: Chlorhexidine is widely used as an antiseptic or disinfectant in both hospital and community settings. A number of bacterial species display resistance to this membrane-active biocide. We examined the transcriptomic response of a representative nosocomial human pathogen, Acinetobacter baumannii, to chlorhexidine to identify the primary chlorhexidine resistance elements. The most highly up-regulated genes encoded components of a major multidrug efflux system, AdeAB. The next most highly overexpressed gene under chlorhexidine stress was annotated as encoding a hypothetical protein, named here as AceI. Orthologs of the aceI gene are conserved within the genomes of a broad range of proteobacterial species. Expression of aceI or its orthologs from several other γ- or β-proteobacterial species in Escherichia coli resulted in significant increases in resistance to chlorhexidine. Additionally, disruption of the aceI ortholog in Acinetobacter baylyi rendered it more susceptible to ...
The use of imipenem antibiotics to treat A. baumannii infections as the premier agents continued well in the 1990s. Despite the growing incidence of resistance, the level of imipenem use remained relatively high. The persistence of its use in clinical settings can be explained by its superior activity against A.baumannii compared to other antimicrobial agents, even with its growing resistance[63]. As the resistance of imipenem continued to grow, another bacteriostatic carbapenem began to be used in greater frequency-meropenem. In addition to be a broad-spectrum agent against gram-negative bacteria, meropenem is also less likely to cause seizures than imipenem[64]. Besides the continued the use of carbapenems, the combination of anti-A.baumannii agents was a major therapeutic strategy employed in the 1990s. The main purpose of these pairings was to put together drugs that would have similar effects as a monotherapy drug of the same class. This usually involved pairing a drug with high resistance ...
From the Staphylococcus aureus, 40% strains presented resistance to oxacillin (MRSA), 96.9% were resistant to penicillin G, 75.8% to erythromycin and 63.3% to ciproflxacin and levoflxacin. S. aureus strains resistant to linezolid (3.9%), daptomycin (4.7%) and vancomycin (2.3%) wer e also found.. When analyzing the susceptibility profile for Klebsiella pneumoniae, it was possible to observe that this strain presented the highest resistance rates to the following antimicrobials: aztreonam, cefepime and cefotaxime (75.2%) and ertapenem (69.7%); and the lowest rate of resistance was 2.6% to amikacin.. Acinetobacter baumannii presented the most worrying susceptibility profile, presenting a resistance frequency of 100% to imipenem; 91.1% to ciprofloxacin, ceftazidime and cefotaxime; 88.8% to cefepime; 86% to meropenem and 82.2% to levofloxacin.. The highest resistance rates to Pseudomonas aeruginosa were 54.3% to ciprofloxacin, 53.1% to levofloxacin and imipenem, and 47% to norfloxacin. While that to ...
The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. In this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species. Wide members of enterobacteriaceae, most commonly Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, and gram-negative non-fermenters Pseudomonas spp. and Acinetobacter baumannii were found to carry these markers. Moreover, at least seventeen variants of bla NDM-type gene differing into one or two residues of amino acids at distinct positions have been reported so far among different species of bacteria
About ContraFect: ContraFect is a biotechnology company focused on the discovery and development of DLAs, including lysins and amurin peptides, as new medical modalities for the treatment of life-threatening, antibiotic-resistant infections. An estimated 700,000 deaths worldwide each year are attributed to antimicrobial-resistant infections. We intend to address life threatening infections using our therapeutic product candidates from our platform of DLAs, which include lysins and amurin peptides. Lysins are a new class of DLAs which are recombinantly produced antimicrobial proteins with a novel mechanism of action associated with the rapid killing of target bacteria, eradication of biofilms and synergy with conventional antibiotics. Amurin peptides are a novel class of DLAs which exhibit broad-spectrum activity against a wide range of antibiotic-resistant Gram-negative pathogens, including P. aeruginosa, Acinetobacter baumannii, and Enterobacter species. We believe that the properties of our ...
Background: Metallo-β-lactamases (MBLs) are bacterial enzymes that hydrolyze carbapems. MBL-producing gram-negative bacilli have been emerging worldwide. In this study, different MBLs were identified in various lung diseases in the japanese clinical hospitals.. Methods: From Jan. 2009 to Dec. 2010, 1618 GNB strains were submitted to the laboratory of 6 general hospitals in Aichi prefecture. Strains demonstrating a high level ceftazidime resistance (MIC, ,128 mg/ml) were subjected to a screening test for MBL production by using disks containing an MBL inhibitor, sodium mercaptoacetic acid (SMA). PCR and sequencing analyses were performed to confirm the types of MBLs and integrases using primers specific for each gene.. Results: Fifty-three strains (34 Pseudomonas aeruginosa, 11 P. putida and 8 Acinetobacter baumannii) were isolated from elderly patients in various wards. These strains were isolated from various respiratory specimens (sputum, pus). By PCR analyses, 35 IMP-1 producers (26 P. ...
METHODS: In silico translation and digestion were performed on 14-25 whole genomes representing strains of Acinetobacter baumannii, Moraxella catarrhalis, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Klebsiella pneumoniae. Peptides constituting theoretical core peptidomes in each were identified. Rapid tryptic digestion was performed; peptides were analyzed by LC-MS/MS and compared with the theoretical core peptidomes. High-confidence core peptides (false discovery rate ,1%) were identified and analyzed with the lowest common ancestor search to yield potential species-specific peptide markers. The species specificity of each peptide was verified with protein BLAST. Further, 1 or 2 pathogens were serially diluted into pooled inflamed BAL, and a targeted LC-MS/MS assay was used to detect 25 peptides simultaneously. ...
Infectious diseases represent an ever-changing field of practice. Virtually each week brings new information, some with the potential to adjust or reinvent the current clinical practice. It is our task as clinicians to browse through field literature, through reports of randomized controlled trials, excerpts of conference proceedings, and to choose relevant scoops with high applicability in patient management.. This supplement brings together four articles relevant to four different areas of medical practice, from HIV infection to Gram-negative bacilli such as Acinetobacter baumannii and Pseudomonas aeruginosa, and interdisciplinary studies on infectious acute maxillary sinusitis.. Non-infectious comorbidities are of utmost importance in the management of HIV infection, as occurrence of kidney impairment, or fractures following decrease in bone mineral density, can significantly impact the quality of life and/or even life expectancy. The study presented at the 11th Edition of the Scientific Days ...
NIAID recently announced a research funding opportunity to develop and/or produce diagnostics to quickly detect the key bacteria responsible for antibacterial-resistant infections in hospital settings. The request for applications (RFA) will support diagnostics research related to one or more of the following types of bacteria: Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species, and extra-intestinal pathogenic Eschericihia coli. NIAID expects to fund 10-15 awards for a total of up to $12 million in 2015, and the maximum length of each award is 5 years ...
Acinetobacter baumannii is well-recognized as an important nosocomial pathogen, however, due to their intrinsic resistance to several antibiotics, treatment options are limited. Synergistic effects between antibiotics and medicinal plants, particularly their active components, have intensively been studied as alternative approaches. Fifty-one ethanol extracts obtained from 44 different selected medicinal plant species were tested for resistance modifying agents (RMAs) of novobiocin against A. baumannii using growth inhibition assay. At 250 μg/ml, Holarrhena antidysenterica, Punica granatum, Quisqualis indica, Terminalia bellirica, Terminalia chebula, and Terminalia sp. that possessed low intrinsic antibacterial activity significantly enhanced the activity of novobiocin at 1 μg/ml (1/8xminimum inhibitory concentration) against this pathogen. Holarrhena antidysenterica at 7.8 μg/ml demonstrated remarkable resistant modifying ability against A. baumannii in combination with novobiocin. The
Acinetobacter junii is a species of bacteria. Its type strain is ATCC 17908. It can be pathogenic. This bacterium has been linked to nosocomial infections including catheter-related blood stream infections and cellulitis. Vaneechoutte, M.; De Baere, T.; Nemec, A.; Musilek, M.; Van Der Reijden, T. J. K.; Dijkshoorn, L. (2008). Reclassification of Acinetobacter grimontii Carr et al. 2003 as a later synonym of Acinetobacter junii Bouvet and Grimont 1986. International Journal of Systematic and Evolutionary Microbiology. 58 (4): 937-940. doi:10.1099/ijs.0.65129-0. PMID 18398198. Bouvet, P. J. M.; Grimont, P. A. D. (1986). Taxonomy of the Genus Acinetobacter with the Recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and Emended Descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. International Journal of Systematic Bacteriology. 36 (2): 228-240. doi:10.1099/00207713-36-2-228. ...
Bacteria of the genus Acinetobacter are ubiquitous in nature. These organisms were invariably susceptible to many antibiotics in the 1970s. Since that time, acinetobacters; have emerged as multiresistant opportunistic nosocomial pathogens. The taxonomy of the genus Acinetobacter underwent extensive revision in the mid-1980s, and at least 32 named and unnamed species have now been described. Of these, Acinetobacter baumannii and the closely related unnamed genomic species 3 and 13 sensu Tjernberg and Ursing (13TU) are the most relevant clinically. Multiresistant strains of these species causing bacteraemia, pneumonia, meningitis, urinary tract infections and surgical wound infections have been isolated from hospitalised patients worldwide. This review provides an overview of the antimicrobial susceptibilities of Acinetobacter spp. in Europe, as well as the main mechanisms of antimicrobial resistance, and summarises the remaining treatment options for multiresistant Acinetobacter infections. © ...
Ver más] As a part of a nationwide study in Spain, 15 clinical isolates of Acinetobacter genomic species 3 (AG3) were analyzed. The main objective of the study was to characterize the ampC genes from these isolates and to determine their involvement in B-lactam resistance in AG3. The 15 AG3 isolates showed different profiles of resistance to ampicillin (range of MICs, 12 to ,256 μg/ml). Nucleotide sequencing of the 15 ampC genes yielded 12 new AmpC enzymes (ADC-12 to ADC-23). The 12 AG3 enzymes showed 93.7 to 96.1% amino acid identity with respect to the AmpC enzyme from Acinetobacter baumannii (ADC-1 enzyme). Eight out of fifteen ampC genes were expressed in Escherichia coli cells under the control of a common promoter, and with the exception of one isolate (isolate 65, which showed lower B-lactam MICs), significant differences in overall B-lactam MICs for E. coli cells expressing AG3 ampC genes were not revealed. No significant differences in ampC gene expression in AG3 clinical isolates ...
The highly variable nature of the internal transcribed spacer region (ITS) has been claimed to represent an ideal target for designing species-specific probes/primers capable of differentiating between closely related Acinetobacter species. However, several Acinetobacter species contain multiple ITS copies of variable lengths, and these include Acinetobacter bereziniae, Acinetobacter guillouiae and Acinetobacter baylyi. This study shows these length variations result from inter-genomic insertion/deletion events (indels) involving horizontal transfer of ITS fragments of other Acinetobacter species and possibly unrelated bacteria, as shown previously by us. In some instances, indel incorporation results in the loss of probe target sites in the recipient cell ITS. In other cases, some indel sequences contain target sites for probes designed from a single ITS sequence to target other Acinetobacter species. Hence, these can generate false positives. The largest of the indels that remove probe sites is 683 bp
Acinetobacter baumannii remains an important and difficult-to-treat pathogen whose resistance patterns result in significant challenges for the clinician. Despite the prevalence and interest in A. baumannii infections, there is relatively limited wellcontrolled scientific data to help the clinician select optimal empirical and subsequent targeted therapy for a variety of infections. We will review the currently available antimicrobial agents and discuss the clinical data supporting the use of the various agents.. ...
Prompt detection of metallo-beta-lactamase (MBL) producing isolates is necessary to prevent their dissemination. Frequency of MBLs producing strains among multidrug resistant (MDR) Acinetobacter species and Pseudomonas aeruginosa was evaluated in critical care Patients using imipenem-EDTA disk method. One hundred MDR Acinetobacter spp. and 42 Pseudomonas aeruginosa were checked for MBL production, from January to June 2001. MBL was produced by 96.6 % of imipenem-resistant Acinetobacter isolates, whereas 100% imipenem-resistant Pseudomonas aeroginosa isolates were MBL producers. Carbapenem resistance in MDR Acinetobacter spp. and Pseudomonas aeruginosa isolates in this study was due to MBLs. This calls for strict infection control measures to prevent further dissemination.
The aim of this study is to determine the frequency of quaternary ammonium compound-resistant genes, including qacA/B, qacC/D, qacE, qacΔE1, qacG, qacJ, and β-lactamase genes, including bla CTX-M, bla TEM, bla SHV, bla OXA-23, bla VIM, bla IMP, bla NDM and bla GIM in 51 isolates of carbapenem-resistant Acinetobacter baumannii collected over a period of 2 years and to determine the MIC of chlorhexidine and quaternary ammonium compound-based disinfectant benzalkonium chloride. The qac and bla genes were detected by the PCR method. The MICs were determined using the broth microdilution method. The MIC of benzalkonium chloride was in the range of 4 to 64 µg l−1 and chlorhexidine in the range of 4 to 64 µg l−1. The qacΔE1 gene was detected in 96.07 % (49/51) isolates and qacE in 31.37 % (16/51), qacG in 23.52 % (12/51) and qacA/B in 13.72 % (7/51). The qacC/D genes and qacJ were not found in any of these strains in this study. The frequency of bla genes was as follows: 84.31 % (43/51) for bla OXA-23
Abstract Background Acinetobacter species have become increasingly common in the intensive care units (ICU) over the past two decades, causing serious infections. At the American University of Beirut Medical Center, the incidence of multi-drug resistant Acinetobacter baumannii(MDR-Ab) [...] ...
The SOS response to DNA damage that induces up to 10% of the prokaryotic genome requires RecA action to relieve LexA transcriptional repression. In Acinetobacter species, which lack LexA, the error-prone polymerase accessory UmuDAb is instead required for ddrR induction after DNA damage, suggesting it might be a LexA analog. RNA-Seq experiments defined the DNA damage transcriptome (mitomycin C-induced) of wild type, recA and umuDAb mutant strains of both A. baylyi ADP1 and A. baumannii ATCC 17978. Of the typical SOS response genes, few were differentially regulated in these species; many were repressed or absent. A striking 38.4% of all ADP1 genes, and 11.4% of all 17978 genes, were repressed under these conditions. In A. baylyi ADP1, 66 genes (2.0% of the genome), including a CRISPR/Cas system, were DNA damageinduced, and belonged to four regulons defined by differential use of recA and umuDAb. In A. baumannii ATCC 17978, however, induction of 99% of the 152 mitomycin C-induced genes depended on recA,
Members of the genus Acinetobacter are ubiquitous in the environment and the multiple-drug resistant species A. baumannii is of significant clinical concern. This clinical relevance is currently driving research on bacterial viruses infecting A. baumannii, in an effort to implement phage therapy and phage-derived antimicrobials. Initially, a total of 42 Acinetobacter phage genome sequences were available in the international nucleotide sequence databases, corresponding to a total of 2.87 Mbp of sequence information and representing all three families of the order Caudovirales and a single member of the Leviviridae. A comparative bioinformatics analysis of 37 Acinetobacter phages revealed that they form six discrete clusters and two singletons based on genomic organisation and nucleotide sequence identity. The assignment of these phages to clusters was further supported by proteomic relationships established using OrthoMCL. The 4067 proteins encoded by the 37 phage genomes formed 737 groups and 974
Multi-drug resistant Acinetobacter New Delhi Metallo-beta-lactamase-1 (NDM-1) is an enzyme that makes bacteria resistant to a broad range of beta-lactam antibiotics. These include the antibiotics of the carbapenem family, which are a mainstay for the treatment of antibiotic-resistant bacterial infection, among those Acinetobacter and more specifically Acinetobacter baumannii. Here are some links to studies that include [...]. ...
Acinetobacter Infections - Pipeline Review, H1 2017 Summary Latest Pharmaceutical and Healthcare disease pipeline guide Acinetobacter Infections - Pipeline
Gram-negative bacteria are a major cause of pulmonary infection in patients with cystic fibrosis (CF).The study aimed to conduct the molecular identification of Gram-negative bacterial flora causing pulmonary infection in children with CF.In this study, sputum samples were taken from 64 CF children undergoing treatment as outpatients or inpatients at a referral childrens hospital in Tehran. The PCR technique was used to detect the presence of Gram-negative bacteria, namely Pseudomonas spp., Acinetobacter baumannii, Stenotrophomonas maltophilia, and Burkholderia cepacia.All samples were positive for 16srRNA. Pseudomonas spp. and A. baumannii were detected in 47% and 14% of the studied samples, respectively. Co-colonization by Pseudomonas spp. and A. baumannii was observed in three (5%) samples.According to this survey, Pseudomonas spp. were the most prevalent Gram-negative bacteria isolated from CF patients with pulmonary infection by molecular assays.
Introduction: Ventilator associated pneumonia and tracheobronchitis (VAP/VAT) due to multiresistant A. baumannii are preeminent causes of mortality and morbidity at ICUs. Inhaled antibiotics, allowing high antibiotic concentrations at airways and secretions, are described as an alternative or complement to systemic therapy.. Objectives: Primary - microbiology outcome on A. baumannii VAP/VAT treatment with inhaled colistin; loss of polymyxin sensitivity after inhaled colistin. Secondary - characterization of clinical outcome; of the A. baumannii isolated; of inhaled colistin use; of systemic antibiotic co-administration; of ICU and hospital mortality.. Material and Methods: Observational, longitudinal, retrospective study, through file consult, of patients admitted at UUM from 01/2005 to 12/2011, with A. baumannii VAP/TAV, treated with inhaled colistin.. Results: 23pts included, 18 male, mean age 70±18 yrs, with mean IUC admission APACHE II and SAPS II score of 23 and 54. Microbiologic ...
Teck Wee Boo, Molecular characterisation of carbapenem resistance of Acinetobacter species in an Irish tertiary care hospital, [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2010, pp 377 ...
Acinetobacter Infections/*epidemiology/*microbiology; Acinetobacter baumannii/classification/drug effects/*genetics/isolation & purification; Adult; Aged; Anti-Bacterial Agents/pharmacology; Asia, Southeastern/epidemiology; Australia/epidemiology; Biofilms/growth & development; Drug Resistance, Multiple, Bacterial/genetics; Female; Genes, Bacterial; Genetic Variation; Genome, Bacterial; Humans; Intensive Care Units; Male; Microbial Sensitivity Tests; Middle Aged; *Molecular Epidemiology; Phylogeny; Type VI Secretion Systems; Virulence/genetics; Virulence Factors/genetics; Whole Genome Sequencing ...
Acinetobacter Infections/*epidemiology/*microbiology; Acinetobacter baumannii/classification/drug effects/*genetics/isolation & purification; Adult; Aged; Anti-Bacterial Agents/pharmacology; Asia, Southeastern/epidemiology; Australia/epidemiology; Biofilms/growth & development; Drug Resistance, Multiple, Bacterial/genetics; Female; Genes, Bacterial; Genetic Variation; Genome, Bacterial; Humans; Intensive Care Units; Male; Microbial Sensitivity Tests; Middle Aged; *Molecular Epidemiology; Phylogeny; Type VI Secretion Systems; Virulence/genetics; Virulence Factors/genetics; Whole Genome Sequencing ...
Abstract: The study compares the bacteriological quality on Asian seabass (Lates calcarifer) between ice and salt storage methods. The main objectives of the study were to identify different bacteria constituents and quantitative bacterial load in Asian seabass when preserved with ice and sea salt. For the purpose of this study, Asian seabass was stored in two different conditions of ice-chilled and salted for 2 days. All fish samples were analyzed by performing bacteriological analysis and the isolated bacteria were identified by using API® identification system. In case of the quantity of bacteria in the flesh, Chilling and salting had no significant difference to the quantity of bacteria on fish flesh. As for the skin, salt-preserved fish showed higher quantity of bacteria than ice-preserved fish. Acinetobacter baumannii and Pseudomonas fluorescens had been identified from skin sample of ice-chilled fish. Besides P. fluorescens and A. baumannii other isolates identified include Vibrio and ...
Lu et al prospectively studied 165 patients with culture-confirmed (bronchoalveolar lavage samples), ventilator-associated pneumonia (VAP) caused by either Pseudomonas aeruginosa or Acinetobacter baumannii.
References. Use of meropenem by continuous infusion to treat a patient with a Bla(kpc-2)-positive Klebsiella pneumoniae blood stream infection. - https://www.ncbi.nlm.nih.gov/pubmed/21859337. Efficacy of high-dose nebulized colistin in ventilator-associated pneumonia caused by multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii. - http://www.ncbi.nlm.nih.gov/pubmed/23132092. ...
Multidrug resistant Gram-negative bacteria (MDRGN bacteria) are a type of Gram-negative bacteria with resistance to multiple antibiotics. They can cause bacteria infections that pose a serious and rapidly emerging threat for hospitalized patients and especially patients in intensive care units. Infections caused by MDR strains are correlated with increased morbidity, mortality, and prolonged hospitalization. Thus, not only do these bacteria pose a threat to global public health, but also create a significant burden to healthcare systems. These bacteria pose a great threat to public health due to the limited treatment options available as well as lack of newly developed antimicrobial medications. MDR strains of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii have become of most concern because they have been reported by hospitals all around the United States. There are many factors which could be contributed to the existence and spread of MDR gram-negative bacteria such as ...
Antibiotic resistance is a major global health challenge. Due to the dry antibiotic discovery pipeline, polymyxins are used as the last-line therapy against Gram- negative superbugs such as Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae. However, current dosing recommendations of parenteral polymyxins are suboptimal, in particular for lung infections due to very limited access of polymyxins into the infection site. Moreover, nephrotoxicity is the dose-limiting factor and can occur in up to 60% of patients. Pulmonary delivery of polymyxins thus holds a great promise for treatment of lung infections with minimum systemic exposure. Unfortunately, there is a lack of information on the pharmacokinetics (PK), pharmacodynamics (PD) and toxicodynamics (TD) for inhaled polymyxins. Worryingly, emergence of polymyxin resistance due to suboptimal use has been increasingly reported worldwide. The overarching aim of this thesis was to utilise antimicrobial PK/PD/TD and ...
Dr. Munoz-Price is a professor of clinical medicine in the Medical College of Wisconsin division of infectious diseases, currently serving as the Enterprise Epidemiologist for Froedtert & the Medical College of Wisconsin. Until 2014, she was the Hospital Epidemiologist for Jackson Memorial Hospital, one of the largest public hospitals in the US. Additionally, Dr. Munoz-Price holds a PhD in Epidemiology from University of Miami. Before working in academia, she worked for 5 years as a private ID consultant in the Greater Chicago area, where she described multicity outbreaks of resistant bacteria throughout the continuum of care. Most of her publications deal with Infection Control, Acinetobacter baumannii, and carbapenemase producing enterics (CRE). Her current research focus deals with the gut microbiome and intestinal pathogens such as Clostridium difficile. With over 70 peer review publications, Dr. Munoz-Prices work has been published in prestigious academic journals, including the New ...
The reassessment of known but neglected natural compounds is a vital strategy for providing novel lead structures urgently needed to overcome antimicrobial resistance. Scaffolds with resistance-breaking properties represent the most promising candidates for a successful translation into future therapeutics. Our study focuses on chelocardin, a member of the atypical tetracyclines, and its bioengineered derivative amidochelocardin, both showing broad-spectrum antibacterial activity within the ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) panel. Further lead development of chelocardins requires extensive biological and chemical profiling to achieve favorable pharmaceutical properties and efficacy. This study shows that both molecules possess resistance-breaking properties enabling the escape from most common tetracycline resistance mechanisms. Further, we show that these compounds are potent ...
Antibiotics are among the most important discoveries of medical science. Analysis of infectious disease mortality data from the U.S. government reveals that antibacterial agents may save over 200,000 American lives annually, and add 5-10 years to U.S. life expectancy at birth. The spread of antibiotic immunity among bacteria – an evolutionary phenomenon mediated by plasmids, transposons, and integrons (carrying DNA encoding attack enzymes, efflux pumps, and other protective devices) – threatens these public health achievements. The examples of increasingly resistant strains of Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa demonstrate the importance of continued development of new antimicrobials, especially ones to treat nosocomial, gram-negative infections. Unfortunately, studies indicate that antibiotics comprise less than 1.5% of compounds under investigation at the largest pharmaceutical and biotechnology companies. Data from ...
HTF MI published a new industry research that focuses on Acinetobacter Infections Treatment market and delivers in-depth market analysis and future prospec
Abstract The CDC estimates that 65-80% of clinically significant drug resistant bacterial infections are drug refractory due to a change in physiological state of pathogens associated with biofilm formation. TRL1068 is a high affinity (100 pM) native human monoclonal antibody (mAb) that disrupts biofilms by extracting a key bacterial scaffolding protein. The epitope is highly conserved in the target protein homologs across a broad spectrum of gram positive and gram negative bacteria, including all ESKAPE pathogens. The released bacteria regain sensitivity to antibiotics. Biofilm disruption has been demonstrated in vitro for Staphylococcus aureus and for several gram negative species: Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae. In vivo, TRL1068 in combination with an antibiotic vs. antibiotic alone has shown statistically significant efficacy against methicillin resistant S. aureus (MRSA) in two animal models (infected implants in mice and infective endocarditis in ...
Results: Among the aerobic organism from meatus and oropharynx, the most frequent isolated strains were alpha-hemolytic Streptococcus (15.4%), followed by coagulase-negative Staphylococcus (14.6%), and Branhamella catarrhalis (13.2%), and the most prevalent isolated strains from sinus were S. aureus (19.1%), Klebsiella pneumonia (16.4%), and B. catarrhalis (15.6%), respectively. The highest antibiotic susceptibility was detected to ciprofloxacin and ceftriaxone in most of the strains; susceptibility to ciprofloxacin ranged from 76.7% (for Pseudomonas aeruginosa) to 100% (for Escherichia coli and Haemophilus influenza); susceptibility to ceftriaxone ranged from 71.4% (for Acinetobacter baumannii) to 100% (for S. pneumonia, Corynebacterium diphtheria, and H. influenza). Besides, regardless of strain, the highest resistance was mostly detected to penicillin (ranging from 33.3% to 91.7%), and to ampicillin (ranging from 38.4% to 83.7%). ...
5Department of Child Development, School of Health, Kırsehir Ahi Evran University, 40100, Kırşehir, Turkey DOI : 10.12991/jrp.2019.182 Salvia L., the largest genus of the family Lamiaceae, is composed of many well-known plants of medicinal value. This study provides the first data on micromorphology, myxocarpy, mineral content and in vitro antimicrobial and antiproliferative activities of mericarps of Salvia pilifera, considered to be a vulnerable endemic species from Turkey. The macro- and micromorphological mericarp traits were documented and illustrated via stereo microscopy and scanning electron microscopy. Mineral content of mericarps was analyzed using ICP-MS. Ethanol extract of mericarps was tested against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Acinetobacter baumannii, Aeromonas hydrophila, Candida albicans, Candida tropicalis, and Candida glabrata using broth microdilution method. Antimycobacterial activity was performed against Mycobacterium tuberculosis H37Rv ...
Ho, Wing Sze and Ou, Hong-Yu and Yeo, Chew Chieng and Thong, Kwai Lin (2015) The dnd operon for DNA phosphorothioation modification system in Escherichia coli is located in diverse genomic islands. BMC Genomics, 16. p. 199. ISSN 1471-2164 Lean, Soo Sum and Suhaili, Zarizal and Yeo, Chew Chieng and Thong, Kwai Lin (2015) Whole genome analyses of an extensive drug-resistant clinical isolate of Acinetobacter baumannii AC12: insights into the mechanisms of resistance of an ST195 clone from Malaysia. International Journal of Antimicrobial Agents, 45 (2). pp. 178-182. ISSN 0924-8579 Lim, King Ting and Yeo, Chew Chieng and Suhaili, Zarizal and Thong, Kwai Lin (2012) Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia. Japanese Journal of Infectious Diseases, 65 (6). pp. 502-509. ISSN 1344-6304 ...
Patient #10, a 47-year-old male who suffered from significant head trauma after a motor vehicle accident. He was initially treated for a subdural hematoma, but soon after receiving surgery for facial fractures, he developed an abscess and ventriculitis caused by a multidrug resistant strain of Acinetobacter baumannii. The patient was in critical condition with dangerously […]. ...
Complementing our current clinical pipeline, we have three additional drug candidates in preclinical development. AR-501 has broad bactericidal activity against Gram-negative and Gram-positive bacteria, including antibiotic-resistant strains. AR-201 is a human IgG1 mAb directed against the F-protein of respiratory syncytial virus (RSV). AR-401 is a mAb discovery program to treat infections caused by the Gram-negative bacterium Acinetobacter baumannii.. ...
NEWARK, NJ - Health professionals investigate bacterial infections in neonatal intensive care at a New Jersey hospital after an early babys death.The State Health Department has found four Acinetobacter baumannii cases at University Hospital in Newark since October 1. The child had the bacteria and was transferred to another facility where the child died. The department says that the exact cause of death is under investigation due to compound medical conditions. The department says it has discovered major deficiencies in the injection control at the hospital and has ordered a correction plan. Bacteria can cause pneumonia or severe blood or wound infections. The cases are not related to the virus that has infected 1 9 patients and was killed seven in a child rehabilitation center in New Jersey. Copyright 2018 Associated Press. All rights reserved. This material may not be published, transmitted, rewritten or redistributed.. ...
Ventricular assist devices (VAD) have benefited patients with end-stage heart failure as a bridge to heart transplantation (HTx). We present our experience of HTx in the presence of device-related infection (DRI) including driveline exit site with pocket infections. From May 1996 to April 2003, mechanical circulatory support with the HeartMate VAD was performed in eight patients, and with the Thoratec VAD in seven patients. Although 151 patients underwent HTx during that period, only 8 of the 15 patients had suitable donors and underwent orthotopic HTx. Six of the eight patients developed DRI. Their ages ranged from 18 to 59 years (mean = 36 +/- 14 years). The duration of VAD support ranged from 8 to 287 days (mean = 125 +/- 117 days). The general condition and cardiac function improved gradually under VAD support. At the time of HTx, all six male patients were suffering from DRI. The causative microorganisms were Acinetobacter baumannii (n = 3) methicillin-resistant Staphylococcus aureus (n = ...
Tilley, D., Premjani, V., Kumar, A., and Samis J. A. (2012) Factor V degradation by Acinetobacter baumannii: part of a novel virulence mechanism to attenuate blood coagulation. In A New Coalition for a Challenging Battlefield: Military and Veteran Health Research E. A. Aiken and S. A. H. Belanger (eds), p215-234. Canadian Defence Academy Press, Kingston, ON, Canada ...
Peptidyl-tRNA hydrolase (Pth) catalyzes the breakdown of peptidyl-tRNA into peptide and tRNA components. Pth from Acinetobacter baumannii (AbPth) was cloned, expressed, purified and crystallized in a native unbound (AbPth-N) state and in a bound state with the phosphate ion and cytosine arabinoside (cytarabine) (AbPth-C). Structures of AbPth-N and AbPth-C were determined at 1.36 and 1.10 Å resolutions, respectively. The structure of AbPth-N showed that the active site is filled with water molecules. In the structure of AbPth-C, a phosphate ion is present in the active site, while cytarabine is bound in a cleft which is located away from the catalytic site. The cytarabine-binding site is formed with residues: Gln19, Trp27, Glu30, Gln31, Lys152, Gln158 and Asp162. In the structure of AbPth-N, the side chains of two active-site residues, Asn70 and Asn116, were observed in two conformations. Upon binding of the phosphate ion in the active site, the side chains of both residues were ordered to ...
Domača sredstva za zdravljenje prostatitisa. Acinetobacter baumannii Ginkgo biloba prostatitis. Testi prostatitis PSA v krvi domača sredstva za zdravljenje adenoma prostate, bolečina v simptome prostatitisa kot za zdravljenje Staphylococcus aureus na gabyfota.magazinmt.ruča trdna limona mila z zelišči Zdravljenju prostatitisa folk pravna sredstva, preventivni ukrepi Folk pravna sredstva za nohte. propolis - čebela lepilo za popravilo panja od znotraj, matični mleček je skrivnost, pridobljena iz čeljusti mladih čebel, Čebelji izdelki za zdravljenje prostatitisa. Raztopite svinjsko maščobo, dodajte 75 g propolisa, 4 g cvetnega prahu in matični mleček, 15 g medu. Mešanico segrejemo do plastičnega stanja in oblikujemo majhne. Apr 05, · Po mojem mnenju bi bila upravicena samo uporaba sok terapije,pa se za to bi rabili nacin,kjer hitro dosezes pravo gabyfota.magazinmt.ru pomeni razlicna doza za razlicen volumen panja.V panj pa bi moral ze vpihniti,ali na kaksen drug nacin dodati ...
April 16, 2010 Identifying essential genes of Acinetobacter baumannii using an antisense expression strategy Irving Phillips MBRS-RISE MS to PhD Scholar Department of Biological Sciences California State University, Los Angeles
Acinetobacter sp. ATCC ® 49467D™ Designation: Genomic DNA from Acinetobacter sp. strain AmMS 248 TypeStrain=False Application:
Twenty-two MDR A. baumannii isolates from Intensive Care Unit (ICU) patients and two reference strains for the European clonal lineages I and II (including 3, 15 and 6 isolates that were resistant, intermediate and susceptible to tigecycline, respectively) were tested. Antimicrobial agents were: tigecycline, levofloxacin, piperacillin-tazobactam, amikacin, imipenem, rifampicin, ampicillin-sulbactam, and colistin. MICs were determined by the broth microdilution method. Antibiotic interactions were determined by chequerboard and time-kill assays. Only antibiotic combinations showing synergism or antagonism in both chequerboard and time-kill assays were accepted as authentic synergistic or antagonistic interactions, respectively ...
Results: Multidrug-resistant Acinetobacter was isolated. She was prescribed meropenem. A. bereziniae was successfully identified by MALDI-TOF MS with an updated library, and 16S rRNA gene sequencing analysis supported the result ...
The Acinetobacter radioresistens strain was isolated in our laboratories from an activated sludge pilot plant. It is able to grow in presence of either phenol or benzoate as the sole carbon and energy source, metabolizing them via the ortho pathway. A proteomic approach to the study of the regulation of these catabolic pathways showed that the expression of most of the catabolic enzymes is modulated by the growth substrate (1, 2). Moreover, satellite proteins were identified (porins, chaperonins), specifically induced by aromatics and probably involved in the uptake of these molecules and in the physiological cell response to their presence. In the present research these results have been extended by a more precise kinetic analysis of the bacterial growth on either an aromatic (phenol or benzoate) or a non-aromatic (acetate) carbon source. From these experiments it can be seen that cultures grown in presence of phenol or acetate show similar specific growth rates (=0.769 and 0.766 h-1 ...
Microbiome analysis and confocal microscopy of used kitchen sponges reveal massive colonization by Acinetobacter, Moraxella and Chryseobacterium ...
The 9th International Symposium on the Biology of Acinetobacter will be held from June 19 - 21, 2013 in Cologne, Germany. Travel grants for up to 20 young scientists are available.
Domain architectures containing the following SCOP superfamilies _gap_,52518,52518,52922,_gap_ in Acinetobacter sp. ADP1. Domain architectures illustrate each occurrence of _gap_,52518,52518,52922,_gap_.
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Acinetobacter radioresistens.
TY - JOUR. T1 - Invasive infection caused by carbapenemresistant acinetobacter soli, Japan. AU - Kitanaka, Hiromitsu. AU - Sasano, Masa Aki. AU - Yokoyama, Satoru. AU - Suzuki, Masahiro. AU - Jin, Wanchun. AU - Inayoshi, Masami. AU - Hori, Mitsuhiro. AU - Wachino, Jun Ichi. AU - Kimura, Kouji. AU - Yamada, Keiko. AU - Arakawa, Yoshichika. N1 - Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 2014/9. Y1 - 2014/9. UR - http://www.scopus.com/inward/record.url?scp=84906260981&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84906260981&partnerID=8YFLogxK. U2 - 10.3201/eid2009.140117. DO - 10.3201/eid2009.140117. M3 - Letter. C2 - 25151987. AN - SCOPUS:84906260981. VL - 20. SP - 1574. EP - 1576. JO - Emerging Infectious Diseases. JF - Emerging Infectious Diseases. SN - 1080-6040. IS - 9. ER - ...