Acinetobacter junii is a species of bacteria. Its type strain is ATCC 17908. It can be pathogenic. This bacterium has been linked to nosocomial infections including catheter-related blood stream infections and cellulitis. Vaneechoutte, M.; De Baere, T.; Nemec, A.; Musilek, M.; Van Der Reijden, T. J. K.; Dijkshoorn, L. (2008). "Reclassification of Acinetobacter grimontii Carr et al. 2003 as a later synonym of Acinetobacter junii Bouvet and Grimont 1986". International Journal of Systematic and Evolutionary Microbiology. 58 (4): 937-940. doi:10.1099/ijs.0.65129-0. PMID 18398198. Bouvet, P. J. M.; Grimont, P. A. D. (1986). "Taxonomy of the Genus Acinetobacter with the Recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and Emended Descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii". International Journal of Systematic Bacteriology. 36 (2): 228-240. doi:10.1099/00207713-36-2-228. ...
The Acinetobacter radioresistens strain was isolated in our laboratories from an activated sludge pilot plant. It is able to grow in presence of either phenol or benzoate as the sole carbon and energy source, metabolizing them via the ortho pathway. A proteomic approach to the study of the regulation of these catabolic pathways showed that the expression of most of the catabolic enzymes is modulated by the growth substrate (1, 2). Moreover, "satellite" proteins were identified (porins, chaperonins), specifically induced by aromatics and probably involved in the uptake of these molecules and in the physiological cell response to their presence. In the present research these results have been extended by a more precise kinetic analysis of the bacterial growth on either an aromatic (phenol or benzoate) or a non-aromatic (acetate) carbon source. From these experiments it can be seen that cultures grown in presence of phenol or acetate show similar specific growth rates (=0.769 and 0.766 h-1 ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Acinetobacter radioresistens.
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Biosurfactant producers are crucial for incremental oil production in microbial enhanced oil recovery (MEOR) processes. The isolation of biosurfactant-producing bacteria from oil reservoirs is important because they are considered suitable for the extreme conditions of the reservoir. In this work, a novel biosurfactant-producing strain Acinetobacter junii BD was isolated from a reservoir to reduce surface tension and emulsify crude oil. The biosurfactants produced by the strain were purified and then identified via electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR-MS). The biosurfactants generated by the strain were concluded to be rhamnolipids, the dominant rhamnolipids were C26H48O9, C28H52O9 and C32H58O13. The optimal carbon source and nitrogen source for biomass and biosurfactant production were NaNO3 and soybean oil. The results showed that the content of acid components increased with the progress of crude oil biodegradation. A glass micromodel test
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Bacteria of the genus Acinetobacter are ubiquitous in nature. These organisms were invariably susceptible to many antibiotics in the 1970s. Since that time, acinetobacters; have emerged as multiresistant opportunistic nosocomial pathogens. The taxonomy of the genus Acinetobacter underwent extensive revision in the mid-1980s, and at least 32 named and unnamed species have now been described. Of these, Acinetobacter baumannii and the closely related unnamed genomic species 3 and 13 sensu Tjernberg and Ursing (13TU) are the most relevant clinically. Multiresistant strains of these species causing bacteraemia, pneumonia, meningitis, urinary tract infections and surgical wound infections have been isolated from hospitalised patients worldwide. This review provides an overview of the antimicrobial susceptibilities of Acinetobacter spp. in Europe, as well as the main mechanisms of antimicrobial resistance, and summarises the remaining treatment options for multiresistant Acinetobacter infections. © ...
Ver más] As a part of a nationwide study in Spain, 15 clinical isolates of Acinetobacter genomic species 3 (AG3) were analyzed. The main objective of the study was to characterize the ampC genes from these isolates and to determine their involvement in B-lactam resistance in AG3. The 15 AG3 isolates showed different profiles of resistance to ampicillin (range of MICs, 12 to ,256 μg/ml). Nucleotide sequencing of the 15 ampC genes yielded 12 new AmpC enzymes (ADC-12 to ADC-23). The 12 AG3 enzymes showed 93.7 to 96.1% amino acid identity with respect to the AmpC enzyme from Acinetobacter baumannii (ADC-1 enzyme). Eight out of fifteen ampC genes were expressed in Escherichia coli cells under the control of a common promoter, and with the exception of one isolate (isolate 65, which showed lower B-lactam MICs), significant differences in overall B-lactam MICs for E. coli cells expressing AG3 ampC genes were not revealed. No significant differences in ampC gene expression in AG3 clinical isolates ...
Optimum conditions for the activity of the new DNA methylase in cell lysate were determined. Methylation of DNAs of bacteriophages λ and T7 and plasmid pBR322 (dcm+) in the 5′-Cm5CWGG-3′ region blocked M.AjnI activity. The specificity of M.AjnI was determined using λ DNA methylated by this enzyme as well as computer modeling and data on the sensitivity of restriction endonucleases Mval, HinfI, and BstMAI to methylation.
COGEM released a comprehensive database of pathogenicity assessment of around 2575 bacterial species in 2011. The database ranks the pathogenicity of species on a scale of 1 to 4. Acinetobacter johnsonii ranks 2 on this scale: Species that can cause diseases in humans or animals, which are unlikely to spread in the human population and for which an adequate prophylaxis or therapy ...
การจำแนกเชื้อ Acinetobacter species สามารถใช้วิธี multiplex PCR เพื่อตรวจหา natural occurring blaOXA gene ที่จำเพาะต่อเชื้อแต่ละ species ได้ ดังนี้ blaOXA23 ในเชื้อ blaOXA134 ในเชื้อ A. lwoffii/A. schindleri, blaOXA211 ในเชื้อ A. johnsonii, blaOXA213 ในเชื้อ A. calcoaceticus, blaOXA214 ในเชื้อ A. haemolyticus, และ blaOXA228 ในเชื้อ A. bereziniae. ...
Three samples of terra rossa were shown to be efficient adsorbents of phosphate [P(V)] from wastewater and removed 29.9-32.6% of P(V). The total iron content in terra rossa was the key factor which determined the P(V) removal from wastewater. The original samples of terra rossa were effective support materials for the immobilization of metabolically active P(V)-accumulating bacteria Acinetobacter junii (0.56-2.47×1010 CFU g-1). The removal of oxalate-extractable iron from original sample of terra rossa increased the number of immobilized bacteria to 1.34×1011 CFU g-1, which is the largest number of immobilized bacteria reported in the literature so far. In reactors containing the A. junii and terra rossa P(V) was removed from wastewater by simultaneous adsorption onto terra rossa and accumulation inside bacterial cells, resulting in 40.5-62.5% of P(V) removal. Terra rossa is a promising substrate for biological P(V) removal from wastewater, acting both as adsorbent of P(V) and carrier of ...
The SOS response to DNA damage that induces up to 10% of the prokaryotic genome requires RecA action to relieve LexA transcriptional repression. In Acinetobacter species, which lack LexA, the error-prone polymerase accessory UmuDAb is instead required for ddrR induction after DNA damage, suggesting it might be a LexA analog. RNA-Seq experiments defined the DNA damage transcriptome (mitomycin C-induced) of wild type, recA and umuDAb mutant strains of both A. baylyi ADP1 and A. baumannii ATCC 17978. Of the typical SOS response genes, few were differentially regulated in these species; many were repressed or absent. A striking 38.4% of all ADP1 genes, and 11.4% of all 17978 genes, were repressed under these conditions. In A. baylyi ADP1, 66 genes (2.0% of the genome), including a CRISPR/Cas system, were DNA damageinduced, and belonged to four regulons defined by differential use of recA and umuDAb. In A. baumannii ATCC 17978, however, induction of 99% of the 152 mitomycin C-induced genes depended on recA,
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Strategy for In Situ Detection of Natural Transformation-Based Horizontal Gene Transfer Events - they used a pUC derived plasmid called pCLT that they got from Palmen and Hellingwerf ...
Acinetobacter sp. ATCC ® 49467D™ Designation: Genomic DNA from Acinetobacter sp. strain AmMS 248 TypeStrain=False Application:
1EO2: Structure of Acinetobacter strain ADP1 protocatechuate 3, 4-dioxygenase at 2.2 A resolution: implications for the mechanism of an intradiol dioxygenase.
1EOC: Structure of Acinetobacter strain ADP1 protocatechuate 3, 4-dioxygenase at 2.2 A resolution: implications for the mechanism of an intradiol dioxygenase.
Based on imipenem resistance in an Acinetobacter genospecies 3 clinical isolate, we were able to identify, for the first time in this genomic species, a plasmid-encoded blaOXA-58 gene that was 100% homologous to the same ...
The World Health Organization has just released its list of the 12 bacteria that pose the greatest risk to humanitys existence, stating that new antibiotics are "urgently needed" to counter them. WHO held a press conference recently to unveil the list of the most dangerous "superbugs," which are resistant to most antibiotics making them difficult or nearly impossible to treat.. The bacteria Acinetobacter baumannii topped the list. This disease can result in pneumonia, blood infections, and more. It affects people with compromised immune systems, and it attacks organ systems with a high fluid content, like the respiratory or urinary tract. ...
Microbial taxonomy remains a conservative discipline, relying on phenotypic information derived from growth in pure culture and techniques that are time-consuming and difficult to standardize, particularly when compared to the ease of modern high-throughput genome sequencing. Here, drawing on th ...
Acinetobacter sp. ATCC ® 49137™ Designation: AmMS 203 TypeStrain=False Application: Quality control strain Quality control strain for MicroScan [Reg TM] products
Domain architectures containing the following SCOP superfamilies _gap_,52518,52518,52922,_gap_ in Acinetobacter sp. ADP1. Domain architectures illustrate each occurrence of _gap_,52518,52518,52922,_gap_.
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Acinetobacter calcoaceticus is a bacterial species of the genus Acinetobacter. It is a nonmotile, gram negative coccobacillus. It grows under aerobic conditions, is catalase positive and oxidase negative. It is part of the normal human intestinal flora. Together with A. baumannii, it is referred to as the A. calcoaceticus-A. baumannii complex, which is relatively simple to identify based on the beforementioned phenotypic characteristics. To identify other Acinetobacter species genotyping is required. A. calcoaceticus is a soil bacterium. It has been shown to be prevalent in the tiger mosquito Aedes albopictus microflora. Phloroglucinol carboxylic acid is a degradation product excreted by A. calcoaceticus grown on (+)-catechin as the sole source of carbon. A. calcoaceticus can be pathogenic and cause an opportunistic infection in patients with multiple underlying diseases. A. calcoaceticus can be used as an alternative to A. baumannii in the laboratory setting. The interchangeability of the two ...
Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows the use of yeast cells for the production of wax esters that are high-value materials and can be used in a variety of industrial applications. The products of WSs include fatty acid ethyl esters, which can be directly used as biodiesel. Here, heterologous WSs derived from five different organisms were successfully expressed and evaluated for their substrate preference in Saccharomyces cerevisiae. We investigated the potential of the different WSs for biodiesel (that is, fatty acid ethyl esters) production in S. cerevisiae. All investigated WSs, from Acinetobacter baylyi ADP1, Marinobacter hydrocarbonoclasticus DSM 8798, Rhodococcus opacus PD630, Mus musculus C57BL/6 and Psychrobacter arcticus 273-4, have different substrate specificities, but they can all lead to the formation of biodiesel. The best biodiesel producing strain was found to be
Acinetobacter calcoaceticus MopR protein: Member of the NtrC family of transcriptional activators with significant homology to XylR and DmpR from Pseudomonas; regulates phenol degradation in Acinetobacter calcoaceticus; has ATP-binding activity; binds phenol; GenBank CAA93242
Acinetobacter Infections - Pipeline Review, H1 2017 Summary Latest Pharmaceutical and Healthcare disease pipeline guide Acinetobacter Infections - Pipeline
Teck Wee Boo, Molecular characterisation of carbapenem resistance of Acinetobacter species in an Irish tertiary care hospital, [thesis], Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2010, pp 377 ...
Genus and Species: Acinetobacter calcoaceticus (strepomycin sensitive) Domain: Prokaryote Optimal Growth Medium: Brain Heart Infusion Agar Optimal Growth Temperature: 30° C Package: MicroKwik Culture® Vial Biosafety Level: 2 Gram Stain: Gram-Negative Shape: Bacillus (rod-shaped)
Domain architecture and assignment details (superfamily, family, region, evalue) for HMPREF0012_00695T0 from Acinetobacter calcoaceticus ruh2202. Plus protein sequence and external database links.
HTF MI published a new industry research that focuses on Acinetobacter Infections Treatment market and delivers in-depth market analysis and future prospec
Complementing our current clinical pipeline, we have three additional drug candidates in preclinical development. AR-501 has broad bactericidal activity against Gram-negative and Gram-positive bacteria, including antibiotic-resistant strains. AR-201 is a human IgG1 mAb directed against the F-protein of respiratory syncytial virus (RSV). AR-401 is a mAb discovery program to treat infections caused by the Gram-negative bacterium Acinetobacter baumannii.. ...
I am interested in DNA repair mechanisms and recombination. These processes are essential for the protection of organisms from DNA-damaging agents such as ultraviolet light and chemical mutagens. These mechanisms also contribute to the evolution of new traits. Projects in my lab involve using genetic and molecular techniques to study DNA repair and recombination in the bacteria Escherichia coli and Acinetobacter baylyi. In E. coli, DNA damage causes the induction of over 20 genes and this is termed the SOS response. Although the SOS system has served as a model for understanding DNA repair and recombination in other systems such as yeast, plants and humans, there is still much that we dont understand about the SOS response in E. coli. For example, we have not yet identified the precise inducing signal of the SOS response or the biological functions of many of the SOS gene products. It is not yet clear whether A. baylyi exhibits an E. coli-like SOS response. Characterization of DNA repair and ...
By Stewart MacInnis. Spectrum Volume 18 Issue 21 - February 22, 1996 John L. Johnson, whose pioneering research on differential Bacteroides species won him international recognition as a microbiologist, died last Friday (Feb. 16) at his Blacksburg home after a long illness. He was 59.. "He was an outstanding scientist, he was an excellent teacher, and he really cared about his work," said W.E.C. Moore, a friend and former colleague of Johnsons. "The world lost a top scientist in microbiology.". Moore, then head of the anaerobe laboratory, brought Johnson to Virginia Tech from his post-doctoral studies at the University of Washington-Seattle in 1968. "John did his best work here," Moore said.. That work earned Johnson the 1980 Bergeys Manual Trust Award, one of the most prestigious international awards given in his field. The Pasteur Institute of Paris named a bacterial species for Johnson, Acinetobacter johnsonii. That, Moore said, is a sign of exceptional respect and an indication of the ...
Resistência aos antibióticos β-lactâmicos em isolados clínicos de Acinetobacter spp : caracterização molecular de novas carbapenemases, IMP-5 e OXA-33, e estudo da relação clonal entre os isolados resistentes ao ...
SUMMARY Steel and Cowan (1964) designated Schaub's Biol. 1 as the type strain for Bacterium anitratum Schaub and Hauber 1948. However, this strain was not included among those originally studied by Schaub and Hauber (1948) and cannot be recognized (Rule 9d, Note b, Intl. Code of Nomenclature of Bacteria). One of the original strains used by Schaub and Hauber (Schaub 81, American Type Culture Collection (ATCC) 19606, RH 2208) is here designated as the type for B. anitratum Schaub and Hauber 1948. The morphology, physiology, and per cent guanine plus cytosine of strain 81 are described and more than sixty characters of the strain recorded. The characteristics of strain 81 were found to be in good agreement with those given in the original description by Schaub and Hauber (1948).
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Accepted name: 2-hydroxycyclohexanone 2-monooxygenase. Reaction: 2-hydroxycyclohexan-1-one + NADPH + H+ + O2 = 6-hydroxyhexan-6-olide + NADP+ + H2O. Systematic name: 2-hydroxycyclohexan-1-one,NADPH:oxygen 2-oxidoreductase (1,2-lactonizing). Comment: the product decomposes spontaneously to 6-oxohexanoic acid (adipic semialdehyde).. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 62628-31-3. References. 1. Davey, J.F. and Trudgill, P.W. The metabolism of trans-cyclohexan-1,2-diol by an Acinetobacter species. Eur. J. Biochem. 74 (1977) 115-127. [PMID: 856571]. ...
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As part of the SENTRY Antimicrobial Surveillance Program, a total of 1078 Acinetobacter species and 842 Stenotrophomonas maltophilia isolates were collected between January 1997 and December 1999 from 5 geographic regions (Canada, the United States, Latin America, Europe, and the Asia-Pacific). The frequency of infections (by geographic region and body site), including those due to imipenem-resistant Acinetobacter species and trimethoprim-sulfamethoxazole (TMP-SMZ)-resistant S. maltophilia, was evaluated. The possibility of seasonal variations in bloodstream infections caused by Acinetobacter species was studied, as was the activity of several therapeutic antimicrobials against all strains. Acinetobacter species and S. maltophilia were most frequently associated with pulmonary infections, independent of the region evaluated. In contrast, patterns of antimicrobial resistance markedly varied among distinct geographic regions, especially for nosocomial isolates. Although the carbapenems were the ...
Acinetobacter calcoaceticus BD413 accumulates wax esters and triacylglycerol under conditions of mineral nutrient limitation. Nitrosoguanidine-induced mutants of strain BD413 were isolated that failed to accumulate wax esters under nitrogen-limited growth conditions. One of the mutants, Wow15 (without wax), accumulated wax when grown in the presence of cis-11-hexadecenal and hexadecanol but not hexadecane or hexadecanoic acid. This suggested that the mutation may have inactivated a gene encoding either an acyl-acyl carrier protein or acyl-coenzyme A (CoA) reductase. The Wow15 mutant was complemented with a cosmid genomic library prepared from wild-type A. calcoaceticus BD413. The complementary region was localized to a single gene (acr1) encoding a protein of 32,468 Da that is 44% identical over a region of 264 amino acids to a product of unknown function encoded by an open reading frame associated with mycolic acid synthesis in Mycobacterium tuberculosis H37Ra. Extracts of Escherichia coli ...
Acinetobacter spp. are a diverse group of Gram-negative bacteria frequently implicated in nosocomial infections. Genotypic methods have been instrumental in studying Acinetobacter, but few offer high resolution, rapid turnaround time, technical ease and high inter-laboratory reproducibility, which has hampered understanding of disease incidence, transmission patterns and diversity within this genus. Here, we further evaluated multilocus PCR electrospray ionization/ mass spectrometry (PCR/ESI-MS), a method that is simple and robust, and provides both species characterization and strain-level resolution of Acinetobacter spp. on a single platform. We examined 125 Acinetobacter isolates from 21 hospitals, laboratories and medical centres spanning four counties in Arizona, USA, using PCR/ESI-MS. We compared PCR/ESI-MS with an in-house amplified fragment length polymorphism (AFLP) genotyping scheme. PCR/ESI-MS demonstrated that Acinetobacter spp. from Arizonan hospitals had similar species and strain
Given the growing interest in the production of new and low cost bioemulsifiers, the rice and wheat bran and straw were investigated in this study for the production of bioemulsifier by Lactobacillus plantarum subsp. plantarum PTCC 1896 (probiotic). The strain produced bioemulsifier only in the rice bran hydrolysate medium. The bioemulsifier amount reached around 0.7 g L-1 for 72 hours of fermentation. The new biomolecule was extracted, purified, and its structural and thermal properties were evaluated. The functional groups and the structure of the molecule were revealed by GPC, FT-IR, 1HNMR and 13CNMR techniques. The bioemulsifier was a water soluble extracellular high molecular weight (|107 Da) α-glucan (81.74%) bound with protein (18.18%). Thermal behavior was studied using DSC and TG analysis. Thermal analysis showed the bioemulsifier broke down above 211.74°C, and the melting point was 182.0°C with the enthalpy value of 101.7 J g-1. These results might provide incentives for the industrial
Given the growing interest in the production of new and low cost bioemulsifiers, the rice and wheat bran and straw were investigated in this study for the production of bioemulsifier by Lactobacillus plantarum subsp. plantarum PTCC 1896 (probiotic). The strain produced bioemulsifier only in the rice bran hydrolysate medium. The bioemulsifier amount reached around 0.7 g L-1 for 72 hours of fermentation. The new biomolecule was extracted, purified, and its structural and thermal properties were evaluated. The functional groups and the structure of the molecule were revealed by GPC, FT-IR, 1HNMR and 13CNMR techniques. The bioemulsifier was a water soluble extracellular high molecular weight (|107 Da) α-glucan (81.74%) bound with protein (18.18%). Thermal behavior was studied using DSC and TG analysis. Thermal analysis showed the bioemulsifier broke down above 211.74°C, and the melting point was 182.0°C with the enthalpy value of 101.7 J g-1. These results might provide incentives for the industrial
Bacterial infections due to Acinetobacter species are typically encountered in health care settings and can be particularly difficult to treat due to the propensity of the organism to incorporate multiple antibiotic resistance mechanisms. Hospital outbreaks of multidrug-resistant Acinetobacter infections have been reported. A wide range of infections are possible with this organism, including bloodstream infections, pneumonia (occasionally even community-acquired pneumonia), urinary tract infections, and wound infections (wound infection in soldiers after traumatic injury have been reported ...
Pseudomonas aeruginosa and Acinetobacter spp. are found to be associated with biofilm and metallo-β-lactamase production and are the common causes of serious infections mainly in hospitalized patients. So, the main aims of this study were to determine the rates of biofilm production and metallo beta-lactamase production (MBL) among the strains of Pseudomonas aeruginosa and Acinetobacter spp. isolated from hospitalized patients. A total of 85 P. aeruginosa isolates and 50 Acinetobacter spp. isolates isolated from different clinical specimens from patients admitted to Shree Birendra Hospital, Kathmandu, Nepal from July 2013 to May 2014 were included in this study. The bacterial isolates were identified with the help of biochemical tests. Modified Kirby-Bauer disc diffusion technique was used for antimicrobial susceptibility testing. Combined disc diffusion technique was used for the detection of MBL production, while Congo red agar method and tube adherence method were used
TY - JOUR. T1 - Comparative genomic analysis of Acinetobacter oleivorans DR1 To determine strain-specific genomic regions and gentisate biodegradation. AU - Jung, Jaejoon. AU - Madsen, Eugene L.. AU - Jeon, Che Ok. AU - Park, Woojun. PY - 2011/10. Y1 - 2011/10. N2 - The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplete glucose metabolism, metabolic pathways of aromatic compounds, biofilm formation, antibiotics and metal resistance, and natural competence genes were conserved in four compared genomes. Interestingly, only strain DR1 possesses gentisate ...
Fig. 1. (a) PFGE analysis of Acinetobacter baumannii strains. (b) Plasmid identification by digestion with S1 nuclease. (c) Hybridization with blaNDM-1 probe. Lanes: 1, A. baumannii AB-I1; 2, AB-I2; 3, AB-I3; 4, AB-I4; 5, AB-I5. Lanes 6 to 8, A. baumannii European clones EC-I (strain RUH-875), EC-II (strain RUH-134), and EC-III (strain RUH-5875), respectively. Bands with white arrows indicate the presence of plasmids without signal hybridization with the blaNDM-1 probe; black arrow indicates the chromosomal position with positive hybridization with the blaNDM-1 probe. ...
Motility plays an essential role in the host–parasite relationship of pathogenic bacteria, and is often associated with virulence. While many pathogenic bacteria use flagella for locomotion, Acinetobacter baumannii strains do not have flagella, but have other features that aid in their motility. To study the genes involved in motility, transposon mutagenesis was performed to construct A. baumannii mutant strains. Mutant strain MR14 was found to have reduced motility, compared to wild-type ATCC 17978. NCBI BLAST analysis revealed that the Tn10 transposon in the MR14 genome is integrated into the gene that encodes for carboxy-terminal processing protease (Ctp). Additionally, MR14 exhibits a mucoidy, sticky phenotype as the result of increased extracellular DNA (eDNA) caused by bacterial autolysis. Transmission and scanning electron microscopy revealed cytoplasmic content leaving the cell and multiple cell membrane depressions, respectively. MR14 showed higher sensitivity to environmental stressors.
Journal of Shahid Sadoughi University of Medical Sciences ماهنامه علمی پژوهشی دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید صدوقی یزد
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Acinetobacter baumannii bacteria, coloured scanning electron micrograph (SEM). This bacterium has developed resistance to a number of antibiotics and is increasingly seen in opportunistic infections in hospitals. It typically infects the lungs, leading to a form of pneumonia. It can develop resistance to antibiotics even as they are being used to treat an infection. Because of this, and because it is generally found in weakened patients, the mortality rate for infections with A. baumannii is high. In healthy individuals, however, it is a normal part of the skin flora. Magnification: x11,000 when printed 10 centimetres wide. - Stock Image B220/1513
Altun, Hatice Uludag and Bulut, Cemal and Sahin, Hunkar and Kinikli, Sami and Yagci, Server and Kudret Adiloglu, Ali and Pekcan Demiroz, Ali (2014) Antimicrobial Susceptibilities of Clinical Acinetobacter baumannii Isolates With Different Genotypes. Jundishapur Journal of Microbiology, 7 (12). p. 1. ISSN 2008-4161 ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Acinetobacter baumannii.
Corrected Genome Sequence of Acinetobacter Baumannii Strain AB0057, an Antibiotic-Resistant Isolate from Lineage 1 of Global Clone 1 ...
Corrected Genome Sequence of Acinetobacter Baumannii Strain AB0057, an Antibiotic-Resistant Isolate from Lineage 1 of Global Clone 1 ...
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Increase Fiber: Foods that are a good source of fiber contain at least 3 grams of fiber per serving. Check the Nutrition Facts label on a food or beverage package to ensure that you are getting at fiber in your diet. Adult men (ages 19-50 years old) need at least 38 grams of fiber, and women of the same age should aim for at least 25 grams per day. The best sources are brown rice, brown pasta, multi-cereal breads, whole grain toast, fruits and vegetables. Legumes such as beans, chickpeas, lentils,etc. are also an excellent source of dietary fiber ...
Prompt detection of metallo-beta-lactamase (MBL) producing isolates is necessary to prevent their dissemination. Frequency of MBLs producing strains among multidrug resistant (MDR) Acinetobacter species and Pseudomonas aeruginosa was evaluated in critical care Patients using imipenem-EDTA disk method. One hundred MDR Acinetobacter spp. and 42 Pseudomonas aeruginosa were checked for MBL production, from January to June 2001. MBL was produced by 96.6 % of imipenem-resistant Acinetobacter isolates, whereas 100% imipenem-resistant Pseudomonas aeroginosa isolates were MBL producers. Carbapenem resistance in MDR Acinetobacter spp. and Pseudomonas aeruginosa isolates in this study was due to MBLs. This calls for strict infection control measures to prevent further dissemination.
Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.
HANIFAH, YASMIN BINTI ABU (2009) Resistant Phenotypes and Genetic Diversity of Nosocomial Acinetobacter baumanii Using Pulsed-Field Gel Electrophoresis in Intensive Care Unit, University Malaya Medical Centre. Full text not available from this repository ...
COVID-19 (short for "Coronavirus Disease 2019) is the disease caused by the novel coronavirus, SARS-CoV-2. Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. COVID-19 represents a particular challenge to people with serious chronic medical conditions such as diabetes. To keep our community as informed as possible, the American Diabetes Association is partnering with the Chan Zuckerberg Initiatives Meta to highlight the latest research developments at the intersection of COVID-19 and diabetes. ...
TY - CHAP. T1 - Desiccation tolerance assays for acinetobacter baumannii. AU - Wang, Xun. AU - Trent, M. Stephen. AU - Davies, Bryan William. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of A. baumannii in clinical settings through contaminated healthcare equipment and personnel. Desiccation is a multifaceted stress, and many physiological and environmental factors can influence its impact on bacterial survival. This chapter provides a protocol for assessing desiccation survival that facilitates comparisons among A. baumannii strains under various environmental conditions.. AB - Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important ...
Anonymous, 2012: Biochemical and Genetic Characterization of Carbapenem-Hydrolyzing beta-Lactamase OXA-229 from Acinetobacter bereziniae
The goal of our study was to link the resistance phenotypes and the genetic determinants of resistance in A. baumannii, giving a "snapshot" of the complex nature of this endemic situation. From these data, it is clear that the potential impact of A. baumannii infections on military and civilian personnel in receiving hospitals is significant.. A unique characteristic of our study is that bloodstream infections were the most common clinical source of Acinetobacter spp. This may reflect the nature and severity of injury among these patients: more than two-thirds of the patients were critically ill, had central venous catheters, and were on mechanical ventilation when their isolates were obtained. Hence, in light of our susceptibility testing, empirical monotherapy for bloodstream infections would be inappropriate. Only two isolates in this collection are resistant to both amikacin and imipenem-cilistatin, and therefore, combination therapy with these antibiotics would ensure that at least one ...
Ochratoxin A (OTA) is a mycotoxin with a main nephrotoxic activity contaminating several foodstuffs. In the present report, five soil samples collected from OTA-contaminated vineyards were screened to isolate microorganisms able to biodegrade OTA. When cultivated in OTA-supplemented medium, OTA was converted in OTα by 225 bacterial isolates. To reveal clonal relationships between isolates, molecular typing by using an automated rep-PCR system was carried out, thus showing the presence of 27 different strains (rep-PCR profiles). The 16S-rRNA gene sequence analysis of an isolate representative of each rep-PCR profiles indicated that they belonged to five bacterial genera, namely Pseudomonas, Leclercia, Pantoea, Enterobacter, and Acinetobacter. However, further evaluation of OTA-degrading activity by the 27 strains revealed that only Acinetobacter calcoaceticus strain 396.1 and Acinetobacter sp. strain neg1, consistently conserved the above property; their further characterization showed that they were
Acinetobacter baumannii remains an important and difficult-to-treat pathogen whose resistance patterns result in significant challenges for the clinician. Despite the prevalence and interest in A. baumannii infections, there is relatively limited wellcontrolled scientific data to help the clinician select optimal empirical and subsequent targeted therapy for a variety of infections. We will review the currently available antimicrobial agents and discuss the clinical data supporting the use of the various agents.. ...
Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity.: Acinetobacter baumannii is an emerging opportunistic pathogen re
Abstract Background Acinetobacter species have become increasingly common in the intensive care units (ICU) over the past two decades, causing serious infections. At the American University of Beirut Medical Center, the incidence of multi-drug resistant Acinetobacter baumannii(MDR-Ab) [...] ...
Five methods for assaying bacterial surface hydrophobicity, namely, bacterial adherence to hydrocarbons, salt aggregation, hydrophobic interaction chromatography, adhesion to polystyrene and latex particle agglutination were used to compare the hydrophobic surface properties of Escherichia coli, Acinetobacter calcoaceticus, Staphylococcus aureus and Streptococcus mitis. Two strains of A. calcoaceticus, including RAG-1, gave strong positive results by all five methods. S. mitis gave weak or negative results by all methods. The results for the other bacteria varied with the method. We conclude that reliance on one method for such tests is inadequate ...
Richmond, G. E., Chua, Kim Lee, Piddock, L. J. V. (2013-03-09). Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide). Journal of Antimicrobial Chemotherapy 68 (7) : 1594-1600. [email protected] Repository. https://doi.org/10.1093/jac/ ...
Lingelsheimia anitrata --, acinetobacter calcoaceticus a species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals. ...
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The main character, Ray, used to be an actor, now making ends meet by collecting the bounty on bringing in wanted men and women. Him and his two bounty hunter friends attend a music festival where a drunk girl says something about going right through the meninges. Theres later talk about insects that feed on other insects by camouflaging themselves and ambushing their victims. The characters are inexplicably drawn to the Mima Mounds National Park for some sightseeing, something which feels unusual to do, yet they do it anyway. Ray has a strange experience in a town near the Mima Mounds - he realises the people and the town dont seem real, theyre just macaroni and glue, and the buildings were cardboard. The criminals they chased, who have since been cut loose by the Canadian authorities for some reason, dont seem human ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Transmissible CRE infections have been recognized for the last two decades. The first major documentation of CRE occurred in Okazaki, Japan, in the 1980s. Today, CRE infections tend to be most common in India and Southeast Asia. Read More. ...
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Theres an outlaw in the waters of Yellowstone National Park: a rainbow trout. The non-native fish has been part of the park for more than a century. But officials are now worried that its pushing
Clinical features and outcomes of Acinetobacter species and Pseudomonas aeruginosa. Presented at the 46th Interscience infections due buy Xanax tablets online UK multidrug resistant baumannii Ab bloodstream infections BSI. Antimicrob Agents Chemother 200751376 378 391 Fournier PE. Clin Infect Dis 1996221026 1032 Carey RB, Banerjee SN. baumannii correlated with an increased 1999 Marmara earthquake. Surveillance cultures and duration of carriage of multidrug resistant Acinetobacter. Seasonal variation of Acinetobacter infections Clin Microbiol Rev 200619257. J Clin Microbiol 2006443623 3627 of resistance to tigecycline has. Crit Care Med 2005331136 1140 of broad spectrum antibiotics. References Schreckenberger PC, Daneshvar with multidrug resistant Pseudomonas aeruginosa. Presented at the 46th Interscience outbreaks of acinetobacter infections, most even more limited. PLoS Genet 20062e7 e7 of Acinetobacter spp. Source Information From Medical for nonsusceptibility in Acinetobacter baumannii. 79 An ...
Carbapenem resistance in A. baumannii is most often associated with class D β-lactamases (OXA-23-like, OXA-40-like and OXA-58-like) and MBLs. OXA-type carbapenemases are predominant in A. baumannii, particularly in worldwide outbreaks of OXA-23 [24]. The molecular analysis of the isolates tested in this study revealed that 14 strains (51.8 %) carried the blaOXA-23-like gene and that two strains carried a blaOXA-24-like gene. All of the strains had a blaOXA-51-like gene, and four strains had a blaOXA-58 gene. In this study, the OXA-58 isolates presented lower MIC values for meropenem than OXA-23-like-positive isolates, which systematically exhibited higher MIC values (Table 1). The isolates with non-acquired OXA genes displayed a marked variation and included some carbapenem-resistant genes. Naturally occurring OXA carbapenemases, such as OXA-51-like enzymes (e.g., OXA 64-66, OXA 68-71, OXA 78-80, OXA-82, OXA-86, OXA-92 and OXA104-112), have been identified in A. baumannii isolates worldwide. In ...
Acinetobacter baumannii causes severe nosocomial infections such as pneumonia, meningitis and sepsis with high mortality rates. This organism represents an increasing danger for immunocompromised adults, especially since there are an increasing number of resistances against antibiotics. Until now, scientific investigation was mainly focused on taxonomy and antibiotic resistance mechanisms. The goal of this project was to analyse the interaction between clinical strains of Acinetobacter baumannii and human cells in order to address the molecular mechanisms causing pathogenicity. Adherence is the first step in colonization of human tissue, and therefore a key event in pathogenesis. To demonstrate the adhesion of bacteria to human cells, a colony counting assay has been established. These experiments used the the type strain of A. baumannii ATCC 19606, as well as clinical isolates. All A. baumannii strains investigated showed adhesion to the lung epithelial cells A549, but the adhesion capacity was ...
Hospital-acquired infections due to Acinetobacter baumannii have become problematic because of high rates of drug resistance. A. baumannii is usually harmless, but it causes sepsis resulting in a high mortality rate in compromised hosts. Therefore, we must consider its interaction with host cells to understand diseases resulting from A. baumannii infection. Neutrophils play a critical role in infective protection against the extracellular growth of bacteria. However, their interactions with A. baumannii remain largely unknown. Recently, a new biological defense mechanism called neutrophil extracellular traps (NETs) has been attracting attention. In the present study, we investigated the responsiveness of human neutrophils to A. baumannii focusing on NET formation. The results demonstrated that infective protection against Pseudomonas aeruginosa via NETs formation was observed, but for A. baumannii NETs formation did not occur. It seems that the innate infective protection against A. baumannii ...
TY - JOUR. T1 - Preclinical advantages of intramuscularly administered peptide A3-APO over existing therapies in Acinetobacter baumannii wound infections. AU - Ostorhazi, Eszter. AU - Rozgonyi, Ferenc. AU - Sztodola, Andras. AU - Harmos, Ferenc. AU - Kovalszky, Ilona. AU - Szabo, Dora. AU - Knappe, Daniel. AU - Hoffmann, Ralf. AU - Cassone, Marco. AU - Wade, John D.. AU - Bonomo, Robert A.. AU - Otvos, Laszlo. PY - 2010/9/1. Y1 - 2010/9/1. N2 - Objectives: The designer antibacterial peptide A3-APO is efficacious in mouse models of Escherichia coli and Acinetobacter baumannii systemic infections. Here we compare the efficacy of the peptide with that of imipenem and colistin in A. baumannii wound infections after burn injury. Methods: CD-1 mice were inflicted with burn wounds and different inocula of A. baumannii, isolated from an injured soldier, were placed into the wound sites. The antibiotics were given intramuscularly (im) one to five times. Available free peptide in the blood and the ...
In February 2006, a patient colonized with a multidrug-resistant sequence type 56 Acinetobacter baumannii strain was admitted to a hospital in Madrid, Spain. This strain spread rapidly and caused a large outbreak in the hospital. Clinicians should be ...
We all know of, or have read, "The Double Helix", James Watsons account of the discovery of the structure of DNA. It is a classic. No need to dwell on the books merits and controversies here; this unorthodox report of scientific competition, brilliant deduction, unlikely collaborations and serendipitous encounters and exchanges between researchers from the US and England is a good read even today.. In the 1950s, top-notch researchers working on the structure of DNA seem to have known each other quite well - and followed each others every move. But it may be less known that Lwoff wrote a most fascinating review of "The Double Helix", analyzing the personalities and the dynamics of the research effort, retelling the story from his vantage point, and providing a psychoanalytical profile of the protagonist and writer, Nobel laureate James Watson himself.. Lwoffs article is so much more than just a book review; it is a very personal account and deep reflection on the personalities involved, about ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Two methods of bioemulsifier separation were applied. The first method was performed according to the procedure described by others [5,8,11,18,19], in which growth the culture was filtered through a filter paper and later through a cellulose membrane. The cell-free medium was transferred to a separatory funnel and extracted with 500 ml of chloroform-methanol solution (2:1). The creamy precipitate formed between the aqueous and the organic phase was collected on cellulose filter and nitrogen stream dried. In the second method, the culture medium was stored at 4°C for 24 h to ensure cell sedimentation. The broth of nitrogen-limited culture on glycerol was separated from cells by centrifugation at 10000×g for 10 min. The resulting cell-free medium was then filtered through a 0.45 µm membrane (Millipore). Approximately 80 ml of the cell-free filtrate was transferred to a separatory funnel and supernatant was acidified to pH 2 with H2SO4 and extracted with 50 ml of ethyl acetate. The aqueous phase ...
ID ACIBC_1_PE11 STANDARD; PRT; 305 AA. AC ACIBC_1_PE11; B2I3S8; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Transposase; (ACIBC_1.PE11). GN Name=insB; OrderedLocusNames=ACICU_p0013; OS ACINETOBACTER BAUMANNII ACICU. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii OC complex. OX NCBI_TaxID=405416; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ACIBC_1.PE11. CC Acinetobacter baumannii ACICU plasmid pACICU1, complete sequence. CC sequence. CC -!- ANNOTATIONS ORIGIN:B2I3S8_ACIBC CC -!- GENE_FAMILY: HOG000023134 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; B2I3S8; -. DR EMBL; CP000864; ACC58990.1; -; Genomic_DNA. DR RefSeq; YP_001840868.1; NC_010605.1. DR ProteinModelPortal; B2I3S8; -. DR STRING; B2I3S8; -. DR GeneID; 6233706; -. DR GenomeReviews; CP000864_GR; insB. DR ...
Antibiotic-Resistant Acinetobacter baumannii Increasing Success Remains a Challenge as a Nosocomial Pathogen. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
BioAssay record AID 534361 submitted by ChEMBL: Antibacterial activity against Acinetobacter baumannii GIL1 isolate harboring intrinsic blaOXA-51/69-like gene by Etest method.
Second cheap ampicillin 500 mg fast delivery antibiotic 83 3147, there is increasing use of chemotherapy both for palliation and buy ampicillin 250mg visa virus.INTRODUCTION. Acinetobacter species have emerged as important hospital-acquired pathogens causing infectious outbreaks in critically-ill patients [1].. Ampicillin omnipen. Review of antibiotic prophylaxis essential for infected joints. Table 2 Procedure Antimicrobial of antibiotic the administration of ampicillin.Looking for a ampicillin? Not a problem! More info about ampicillin Guaranteed Worldwide Shipping Discreet Package Low Prices 24/7/365 Customer Support 100 ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii ...
1) Magnet S, et al. (2001) Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454.. Antimicrob Agents Chemother 45(12):3375-80 PubMed: 11709311 ...
1) Magnet S, et al. (2001) Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454.. Antimicrob Agents Chemother 45(12):3375-80 PubMed: 11709311 ...
Affiliation:国際医療福祉大学,臨床医学研究センター,医師, Research Field:Cerebral neurosurgery,Nerve anatomy/Neuropathology, Keywords:ラット,神経分化,nerve growth factor,brain-derived neurotrophic factor,neuronal differentiation,細胞分裂,アドリアマイシン,アントラサイクリン,ニワトリ,gene transfer, # of Research Projects:4, # of Research Products:0
A key review article has been published in the Journal of Hospital Infection summarising the accumulating evidence that the status of the prior room occupant can influence the chances of the incoming occupant picking up an MDRO. The bottom line is that if you are admitted to a room previously occupied by a patient with an MDRO (including MRSA, VRE, C. difficile and Acinetobacter sp.), you are twice as likely to acquire that MDRO. The impact of these findings is clear: we need to improve standards of discharge disinfection to protect the incoming occupant. So how can we do this? There are several paths to follow towards the same end - and these are not mutually exclusive:. Improve the cleaning / disinfection process ...
3 died in hospital outbreak possibly caused by scopes - AP News: PASADENA, Calif. (AP) - At least three patients died last .02/21/2018 21:44:18PM EST.