TY - JOUR. T1 - Correlation of Red Blood Cell Acetylcholinesterase Enzyme Activity with Various RBC Indices. AU - Gupta, Shalvika. AU - Belle, Vijetha Shenoy. AU - Kumbarakeri Rajashekhar, Ramya. AU - Jogi, Sushma. AU - Prabhu, RV Krishnananda. PY - 2018/9/4. Y1 - 2018/9/4. N2 - Cholinesterases belongs to class hydrolases. There are two types acetylcholinesterase and butyryl cholinesterase. Acetylcholinesterase present in nerve endings and also in the RBC membrane. It helps to maintain the shape and size of RBCs. Any change in shape and size of RBCs may affect the activity of Acetylcholinesterase. Thus this study aimed to estimate RBCs Acetylcholinesterase enzyme activity in various types of anemias and correlate the RBCs Acetylcholinesterase enzyme activity with various hematological indices such as Erythrocyte Sedimentation Rate (ESR), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Red cell Distribution Width (RDW) etc. After ...
Free Online Library: AhR-mediated effects of dioxin on neuronal acetylcholinesterase expression in vitro.(Research, Report) by Environmental Health Perspectives; Health, general Environmental issues Acetylcholinesterase Chlorocarbons Analysis Dibenzofurans Dichloropropane Dioxin Health aspects Dioxins Enzymes Neurons
Define acetylcholinesterase. acetylcholinesterase synonyms, acetylcholinesterase pronunciation, acetylcholinesterase translation, English dictionary definition of acetylcholinesterase. n. An enzyme in the blood and in certain tissues that catalyzes the hydrolysis of acetylcholine. n an enzyme in nerve cells that is responsible for the...
Objective:To investigate the effect of YiShenJiangZhuo decoction on intracerebral AchE activity of ischemia reperfusion rat.Methods:After preparing a model of ischemia-reperfusion based on hypotension,we observed the praxiology of rat by using water labyrinth test and AchE activity of hippocampus by colorimetric method.Results:The model rat had distinct disturbances of learning and remembrance the Ache activity increased.The result of learning and remembrance in YiShenJiangZhuo decoction group improved significantly (P0.01)compared with the model rat;And AchE activity in YiShenJiangZhuo decoction group significaitly decreased(P0.05).Conclusion:It is suggested that the YiShenJiangZhuo decoction can improve the learning and remembrance by decreasing the AchE activity of ischemia reperfusion
In non-neuronal contexts, ACh (acetylcholine) is thought to be involved in the regulation of vital cell functions, such as proliferation, differentiation, apoptosis and cell-cell interaction. In airways, most cells express the non-neuronal cholinergic system, each containing a specific set of components required for synthesis, signal transduction and ACh hydrolysis. The aim of the present study was determine the expression of cholinergic system components in bronchial aspirates from control subjects and patients with lung cancer. We conducted an analysis of cholinergic components in the stored soluble and cellular fraction of bronchial aspirates from non-cancerous patients and patients diagnosed with lung cancer. The results show that the fluid secreted by human lung cells contains enough AChE (acetylcholinesterase) activity to control ACh levels. Thus these findings demonstrate that: (i) AChE activity is significantly lower in aspirates from squamous cell carcinomas; (ii) the molecular ...
Irreversible inhibition of the essential nervous system enzyme acetylcholinesterase by organophosphate nerve agents and pesticides may quickly lead to death. Oxime reactivators currently used as antidotes are generally less effective against pesticide exposure than nerve agent exposure, and pesticide exposure constitutes the majority of cases of organophosphate poisoning in the world. The current lack of published structural data specific to human acetylcholinesterase organophosphate-inhibited and oxime-bound states hinders development of effective medical treatments. We have solved structures of human acetylcholinesterase in different states in complex with the organophosphate insecticide, paraoxon, and oximes. Reaction with paraoxon results in a highly perturbed acyl loop that causes a narrowing of the gorge in the peripheral site that may impede entry of reactivators. This appears characteristic of acetylcholinesterase inhibition by organophosphate insecticides but not nerve agents. ...
Molecular isoform distribution and glycosylation of acetylcholinesterase are altered in brain and cerebrospinal fluid of patients with Alzheimers disease
An acetylcholinesterase has been purified from Torpedo californica by mild proteolysis of electroplax membranes with trypsin (5 µg/ml for 5 min) to solubilize the enzyme, followed by affinity chromatography of the soluble enzyme. The procedure yields an apparently homogeneous enzyme whose molecular weight (approximately 335,000), frictional coefficient (1.65), and amino acid composition all distinguished the Torpedo acetylcholinesterase from that which has been prepared by lytic procedures from Electrophorus. The enzyme is composed of four similar, if not identical, subunits, each of which possesses a catalytic and inhibitor binding site. Torpedo acetylcholinesterase contains 7.9% carbohydrate present as hexoses, hexosamines, and sialic acid, and at least some of these residues are exposed on the outer surface of the molecule. Concanavalin A, a plant lectin with specificity toward mannose residues at nonreducing positions, forms a sedimentable complex with the purified acetylcholinesterase ...
Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa. Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects. This antibody is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and erythrocyte AChE. There is a weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE ...
Background: Neuropsychological studies have extensively described the presence of cognitive dysfunction in MS patients. One possible pharmacological treatment of the impairment could be based on acetylcholinesterase inhibitors (AChEIs), which have sh
2HA4: Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding
The bovine acetylcholinesterase (BoAChE) gene was cloned from genomic DNA and its structure was determined. Five exons coding for the AChE T-subunit and the alternative H-subunit were identified and their organization suggests high conservation of structure in mammalian AChE genes. The deduced amino acid sequence of the bovine T-subunit is highly similar to the human sequence, showing differences at 34 positions only. However, the cloned BoAChE sequence differs from the published amino acid sequence of AChE isolated from fetal bovine serum (FBS) by: (1) 13 amino acids, 12 of which are conserved between BoAChE and human AChE, and (2) the presence of four rather than five potential N-glycosylation sites. The full coding sequence of the mature BoAChE T-subunit was expressed in human embryonal kidney 293 cells (HEK-293). The catalytic properties of recombinant BoAChE and its reactivity towards various inhibitors were similar to those of the native bovine enzyme. Soluble recombinant BoAChE is ...
Acetylcholinesterase (AChE) is a tetrameric serine hydrolase that rapidly catalyzes the hydrolysis of acetylcholine to acetate and choline. The breakdown of acetylcholine is critical for the termination of impulse transmissions at cholinergic synapses within the nervous system. Progressive loss of cholinergic neurons in Alzheimers disease (AD) patients results in severe memory loss and impairment of cognitive function. AChE inhibitors are a strategic approach to symptomatic treatment for AD, since AChE inhibitors increase the levels of acetylcholine in the synapse, thereby enhancing cholinergic activity in the affected regions of the brain. AChE also plays an important role in agriculture since modifications in AChE can confer resistance to pesticides. AChE is a key component in many snake venoms, and AChE staining is routinely used for the initial diagnosis of Hirschsprungs disease, a congenital disorder caused by the absence of ganglion cells in the distal colon ...
Recombinant Acetylcholinesterase (AChE) Protein. Species: Mouse (Murine). Source: Escherichia coli (E. coli). Order product ABIN6301593.
The peripheral anionic site on acetylcholinesterase (AChE), located at the active center gorge entry, encompasses overlapping binding sites for allosteric activators and inhibitors; yet, the molecular mechanisms coupling this site to the active center at the gorge base to modulate catalysis remain unclear. The peripheral site has also been proposed to be involved in heterologous protein associations occurring during synaptogenesis or upon neurodegeneration. A novel crystal form of mouse AChE, combined with spectrophotometric analyses of the crystals, enabled us to solve unique structures of AChE with a free peripheral site, and as three complexes with peripheral site inhibitors: the phenylphenanthridinium ligands, decidium and propidium, and the pyrogallol ligand, gallamine, at 2.20-2.35 A resolution. Comparison with structures of AChE complexes with the peptide fasciculin or with organic bifunctional inhibitors unveils new structural determinants contributing to ligand interactions at the ...
The long article for discussion on the August 31, 2018 #GeriMedJC will take a look at Acetylcholinesterase inhibitors and risk of stroke and death in people with dementia.. Remember during the live hour, you can view the presentation live via Zoom https://zoom.us/j/102282147 . Have you missed our previous sessions? The 2018 presentations are all available on YouTube (click the Subscribe button!): https://www.youtube.com/channel/UC0lfYRt-7pBKFG_81JHUyWg. Acetylcholinesterase inhibitors and risk of stroke and death in people with dementia. Alzheimers Dement. 2018 Jul;14(7):944-951. INTRODUCTION ...
2JGJ: Crystal Structures of Acetylcholinesterase in Complex with Organophosphorus Compounds Suggest that the Acyl Pocket Modulates the Aging Reaction by Precluding the Formation of the Trigonal Bipyramidal Transition State.
TY - JOUR. T1 - Prediction of the binding site of 1-benzyl-4-[(5,6-dimethoxy-1-indanon-2-yl)methyl]piperidine in acetylcholinesterase by docking studies with the SYSDOC program. AU - Pang, Yuan-Ping. AU - Kozikowski, Alan P.. PY - 1994/12. Y1 - 1994/12. N2 - In the preceding paper we reported on a docking study with the SYSDOC program for predicting the binding sites of huperzine A in acetylcholinesterase (AChE) [Pang, Y.-P. and Kozikowski, A.P., J. Comput.-Aided Mol. Design, 8 (1994) 669]. Here we present a prediction of the binding sites of 1-benzyl-4-[(5,6-dimethoxy-1-indanon-2-yl)methyl]piperidine (E2020) in AChE by the same method. E2020 is one of the most potent and selective reversible inhibitors of AChE, and this molecule has puzzled researchers, partly due to its flexible structure, in understanding how it binds to AChE. Based on the results of docking 1320 different conformers of E2020 into 69 different conformers of AChE and on the pharmacological data reported for E2020 and its ...
Two novel families of dual binding site acetylcholinesterase (AChE) inhibitors have been developed, consisting of a tacrine or 6-chlorotacrine unit as the active site interacting moiety, either the 5,6-dimethoxy-2-[(4-piperidinyl)methyl]-1-indanone fragment of donepezil (or the indane derivative thereof) or a 5-phenylpyrano[3,2-c]quinoline system, reminiscent to the tryciclic core of propidium, as the peripheral site interacting unit, and a linker of suitable length as to allow the simultaneous binding at both sites. These hybrid compounds are all potent and selective inhibitors of human AChE, and more interestingly, are able to interfere in vitro both formation and aggregation of the beta-amyloid peptide, the latter effects endowing these compounds with the potential to modify Alzheimers disease progression ...
Due to the diversity of biological activities that can be found in aquatic ecosystems, marine metabolites have been an active area of drug discovery for the last 30 years. Marine metabolites have been found to inhibit a number of enzymes important in the treatment of human disease. Here, we focus on marine metabolites that inhibit the enzyme acetylcholinesterase, which is the cellular target for treatment of early-stage Alzheimers disease. Currently, development of anticholinesterase drugs with improved potency, and drugs that act as dual acetylcholinesterase and amyloid-β aggregation inhibitors, are being sought to treat Alzheimers disease. Seven classes of marine metabolites are reported to possess anti-cholinesterase activity. We compared these metabolites to clinically-used acetylcholinesterase inhibitors having known mechanisms of inhibition. We performed a docking simulation and compared them to published experimental data for each metabolite to determine the most likely mechanism of
Complete information for COLQ gene (Protein Coding), Collagen Like Tail Subunit Of Asymmetric Acetylcholinesterase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Vol 14: Acetylcholinesterase overexpression mediated by oncolytic adenovirus exhibited potent anti-tumor effect.. This article is from BMC Cancer, volume 14.AbstractBackground: Acetylcholinesterase (AChE) mainly functions as an effici. Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
In the fetal and neonatal monkey, periodically organized regions of high activity of acetylcholinesterase were found in the visual cortical area V2 (Area 18). The acetylcholinesterase bands, like the thin and thick stripes of cytochrome oxidase, were found to run orthogonal to the area 17/18 border. During neonatal development these bands progressively narrow and finally disappear shortly after four months of age.
Small numbers of postganglionic cells persist in sympathetic ganglia after the treatment of newborn animals with the nerve growth factor antiserum. In the present study the possibiity was explored whether the cells which survive the immunosympathectomy may be predominantly those which have been designated, in the cat, as cholinergic sympathetic cells. The acetylcholinesterase activity of sympathetic ganglia (superior cervical, stellate, thoracic chain, superior mesenteric, celiac and cardiac) from immunosympathectomized and control rats was visualized by the histochemical method of Koelle. As in the cat, the sympathetic ganglia of rats contain postganglionic cell bodies with either marked, moderate or slight acetylcholinestrase activity. Contrary to cat sympathetic ganglia, the cells with marked and moderate acetylcholinesterase activity predominate in the sympathetic ganglia of control rats. In the various sympathetic ganglia studied in this investigation, cells with slight acetylcholinesterase ...
Acetylcholinesterase小鼠单克隆抗体[HR2](ab2803)可与小鼠, 兔, 豚鼠, 牛, 猫, 人, 猕猴样本反应并经IP, ELISA, IHC, Flow Cyt, ICC/IF实验严格验证,被3篇文献引用。
inproceedings{BUT7539, author="Lukáš {Fujcik} and Radimír {Vrba} and Jan {Krejčí} and Jiří {Háze} and Michal {Skočdopole} and Ondřej {Sajdl}", title="INPUT CONTROL OF SENSOR PARAMETERS AND REPRODUCIBILITY OF SENSOR MEASUREMENT", annote="Fast detection of pesticide toxicity in the field conditions is very important in many aspects (longtime influence of low concentrations on man´s health, pesticide storage, price of one test etc.). The pesticide concentration measurements are performed on a biosensor with acetylcholinesterase (AChE). The AChE is a very sensitive compound. The measurement uses principle of an artificial synapse (AS) and inhibition of an enzyme Acetylcholinesterase (AChE). The AS is a very effective detector of the pesticide toxicity. Therefore properties of the sensor have to be measured without AChE at first. This process is called input control of the sensor. The reproducibility of electrochemical detector is studied first using hydrogen peroxide and second ...
Acetylcholinesterase (HGNC symbol ACHE), also known as AChE or acetylhydrolase, is the primary cholinesterase in the body. It is an enzyme that catalyzes the breakdown of acetylcholine and of some other choline esters that function as neurotransmitters. AChE is found at mainly neuromuscular junctions and in chemical synapses of the cholinergic type, where its activity serves to terminate synaptic transmission. It belongs to carboxylesterase family of enzymes. It is the primary target of inhibition by organophosphorus compounds such as nerve agents and pesticides. AChE is a hydrolase that hydrolyzes choline esters. It has a very high catalytic activity - each molecule of AChE degrades about 25000 molecules of acetylcholine (ACh) per second, approaching the limit allowed by diffusion of the substrate. The active site of AChE comprises 2 subsites - the anionic site and the esteratic subsite. The structure and mechanism of action of AChE have been elucidated from the crystal structure of the enzyme. ...
The researchers) demonstrate that the active component of marijuana, Delta9-tetrahydrocannabinol (THC), competitively inhibits the enzyme acetylcholinesterase (AChE) as well as prevents AChE-induced amyloid beta-peptide (Abeta) aggregation, the key pathological marker of Alzheimers disease. Computational modeling of the THC-AChE interaction revealed that THC binds in the peripheral anionic site of AChE, the critical region involved in amyloidgenesis. Compared to currently approved drugs prescribed for the treatment of Alzheimers disease, THC is a considerably superior inhibitor of Abeta aggregation, and this study provides a previously unrecognized molecular mechanism through which cannabinoid molecules may directly impact the progression of this debilitating disease. ...
Has anyone had any luck using acetylcholinesterase inhibitors like razadyne/ aricept to improve cognitive function? My Doc prescribed razadyne, but insurance wont cover it unless I get a DX of Alzheimers. Im not willing to do that. I saw and ...
Characterization and gene cloning of acetylecholinesterase (AChE) in the insecticide-resistant (R) and -susceptible (S) insects have been reported in the past. However, the studies focused mostly on herbivorous pests, rather than predacious species, such as ladybird beetles. Using R and S Propylaea japonica (thunberg), a full-length cDNA sequence (2928 bp) of the ace1-type AChE gene was determined for the first time. The ace1 encoding a protein of 645 amino acids contained typical conserved motifs, such as FGESAG domains, catalytic triad, acyl pocket, oxyanino hole, choline binding site, peripheral anionic site, omega loop and conserved aromatic residues. R P. japonica displayed 50-times greater resistance to chlorpyrifos or mathamidophos with a significantly lower AChE sensitivity to paraoxon, malaoxon, chlorpyrifos or methamidophos than its S counterpart. Five amino acids in the ace1 of R P. japonica differed from those found in S P. japonica. One of them, F358S, located in the acyl-binding ...
Silver, Ann - 07 The Acetylcholine System: Mapping the Enzymes, Choline Acetyltransferase (ChAT) and Acetylcholinesterase (AchE) (mp3 ...
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We have developed a highly selective sensitive fluorescent detection of acetylcholine (ACh) using bovine serum albumin (BSA) protected atomically precise clusters of gold. The gold quantum clusters ([email protected]) synthesized using bovine serum albumin and conjugated with acetylcholinesterase (AChE), an enzyme specific for acetylcholine, resulting in [email protected] The enzyme, AChE hydrolyzes acetylcholine (ACh) to choline (Ch) which in turn interacts with [email protected] and quenches its fluorescence, enabling sensing. We have carried out the real time monitoring of the hydrolysis of ACh using electrospray ionization mass spectrometry (ESI MS) to find out the mechanism of fluorescent quenching. The validity of present method for determination of concentration of acetylcholine in real system such as blood was demonstrated. Further, the sensor, [email protected] can be easily coated on paper and an efficient and cheap sensor can be developed and detection limit for ACh is found to be 10nM. The fluorescent ...
From a pharmacological perspective, these substances act via the parasympathetic nervous system (PSNS) as reversible inhibitors of the enzyme acetylcholinesterase (AChE). Typically, acetylcholine (ACh) is released into the neural synaptic cleft and binds to ACh receptors post-synapse, which then relays a signal from the nerve. AChE halts the signal transmission via hydrolysis of ACh into acetate and choline. AChE functions rapidly and has a very high catalytic activity - it has been demonstrated that AChE can degrade about 25,000 molecules of ACh per second. Choline is then taken up by the presynaptic nerve and ACh is constructed from choline and Acetyl Coenzyme A (acetyl-CoA) via another enzyme, choline acetyltransferase. As AChE inhbitors, aldicarb and carbofuran inhibit or slow down the normal function of this enzymatic pathway of ACh which results in an accumulation of ACh in the neural synaptic cleft. This accumulation can result in devastating consequences for the organism. ...
Acetylcholinesterase (AChE) exhibits functions unrelated to the catalysis of acetylcholine (ACh) in particular during development. Although the underlying mechanism(s) is presently unknown, a candidat
Various types of acetylcholinesterase (AChE) subunits are characterized by specific C-terminal peptides. In Torpedoand mammals, alternative exons encode H peptides, which contain a C-terminal signal...
Lanks, K W.; Dorwin, J M.; and Papirmeister, B, "Increased rate of acetylcholinesterase synthesis in differentiating neuroblastoma cells." (1974). Subject Strain Bibliography 1974. 492 ...
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Brain Protex with Huperzine (60 caps)-Benefits:May help block the enzyme acetylcholinesterase, helping to prevent the breakdown of acetylcholine, an i
Alzheimers disease may be the top reason for dementia Among the many aged, and with the ever-growing size of this population, situations of Alzheimers disease are expected to triple about the next fifty decades. Consequently, the development of treatments that gradual or halt the disease progression became very important to both of those Enhance the Standard of living for sufferers and lessen the wellness care prices attributable to Alzheimers disease. Here, we demonstrate which the Lively component of marijuana, Delta9-tetrahydrocannabinol (THC), competitively inhibits the enzyme acetylcholinesterase (AChE) together with prevents AChE-induced amyloid beta-peptide (Abeta) aggregation, The crucial element pathological marker of Alzheimers disease ...
OGUNDELE, Olalekan Michael and ADEKEYE, Adeshina Oloruntoba and ADENIYI, Philip Adeyemi and OGEDENGBE, Oluwatosin Olalekan and ENYE, Linus Anderson and SAHEED, Sabiu and ANOSIKE, Victor Ikenna (2013) P53, Bax and Cathepsin D Dysregulation in Neurons Subjected to Cyanide Toxicity and Oxygen Deprivation. World J Life Sci. and Medical Research, 3 (1). pp. 1-6. ISSN 2249-0574 Obajuluwa, Adejoke Olukayode and Akinyemi, Ayodele Jacob and Afolabi, Olakunle Bamikole and Adekoya, Khalid and Sanya, Joseph Olurotimi and Ishola, Azeez Olakunle (2017) Exposure to radio-frequency electromagnetic waves alters acetylcholinesterase gene expression, exploratory and motor coordinationlinked behaviour in male rats. Toxicology Reports, 4. pp. 1-5. ISSN 2214-7500 Ogundele, O. M. and Olaniyan, T. O. and Dare, B. J. and Omotoso, D. R. and Omotosho, O. D. and Akintayo, O. C. and Memudu, A. E. and Caxton-Martins, E. A. (2012) Neuroprotective Properties of NMDA R1 Antagonist (Ketamine) in Cyanide Treated Neurons in vitro. ...
An example of an effective prokinetic agent would be represented by muscarinic receptor agonist or by inhibitors of the enzyme acetylcholinesterase ...
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Dr Douglas Caruana, who carried out the experiments, stated: Disruptions in cholinergic signaling in the prefrontal cortex are known to affect how the mind encodes enduring associations between objects and areas, and a depletion of brain acetylcholine levels in the cortex can be a traditional hallmark of Alzheimers dementia. Professor Bashir added: Acetylcholinesterase inhibitors will be the most widely used medication to treat individuals with Alzheimers dementia and the improvement of synaptic plasticity by acetylcholinesterase inhibition that we now demonstrate may be a way in which these drugs provide clinical efficacy.In addition, infections invariably happen in patients with urinary catheters. All invasive procedures carry the risk of organ damage and can increase health care costs due to medical interventions and longer hospital stays. The Abreu btt 700 system instead relies upon continuous, noninvasive temperature measurement that enables clinicians to get rid of these complications ...
Adenosine 5-triphosphate (ATP) functions as an extracellular signaling molecule that is co-stored and co-released with neurotransmitters at central and peripheral neuronal synapses. Stimulation by ATP up-regulates the expression of synaptic genes in muscle - including the genes for nicotine acetylcholine receptor (α-, δ- and ϵ-subunits) and acetylcholinesterase (AChE) - via the P2Y receptor (P2YR), but the trophic response of neurons to the activation of P2YRs is less well understood. We reported that cultured cortical neurons and the developing rat brain expressed different types of P2YRs, and among these the UTP-sensitive P2Y2R was the most abundant. P2Y2R was found to exist in membrane rafts and it co-localized with the post-synaptic protein PSD-95 in cortical neurons. Notably, agonist-dependent stimulation of P2Y2R elevated the neuronal expression of cholinergic genes encoding AChE, PRiMA (an anchor for the globular form AChE) and choline acetyltransferase, and this induction was ...
Mouse monoclonal Acetylcholinesterase antibody [4E11] validated for WB, ELISA and tested in Human. Referenced in 1 publication. Immunogen corresponding to…
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Brigita Mányová Supervisor: PharmDr. Marie Vopršalová, CSc. Supervisor-specialist: PharmDr. Vendula Hepnarová, Ph.D. Title of diploma thesis: Development of insecticides inhibiting acetylcholineseterase Pest insects cause problems and damage all over the world. They are vectors of serious diseases such as malaria, dengue fever, yellow fever, Zika virus disease and chikungunya disease. They cause huge damage to agricultural crops and are annoying to everyday life in living spaces. The aim of this diploma thesis was in vitro testing of selected compounds from the group of bis-isoquinoline and bispyridinium acetylcholinesterase inhibitors as potential insecticides. Another goal was also to create relationships between structure and effect. The ability of these compounds to inhibit both human (hAChE) and fly acetylcholinesterase (MdAChE) was evaluated. The modified Ellman ...
M. Bartolini, V. Cavrini, V. Andrisano Journal of Chromatography A, 1144 (2007) 102-110 The aim of the present study was the application of a human
Morel, N., S. Bon, H. M. Greenblatt, D. Van Belle, S. J. Wodak, J. L. Sussman, J. Massoulié, and I. Silman, Effect of mutations within the peripheral anionic site on the stability of acetylcholinesterase., Mol Pharmacol, vol. 55, issue 6, pp. 982-92, 1999 Jun. ...