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Congress is not expected to accomplish much before the general election season begins in earnest this summer, but there is broad bipartisan support for accelerating the discovery, development, and delivery of promising new therapies.
Funding levels reported in the Fiscal Year 2015 Intra-Agency Collaborations Reporting System (CRS) may not be consistent with funding levels reported under the Estimates of Funding for Various Research, Condition, and Disease Categories (RCDC) at the NIH Office of Budget Website at https://report.nih.gov/categorical_spending.aspx. The current process, implemented in 2008 through the Research, Condition, and Disease Categorization (RCDC) system, uses sophisticated text data mining (categorizing and clustering using words and multiword phrases) in conjunction with NIH-wide definitions used to match projects to categories. RCDC use of data mining improves consistency and eliminates the wide variability in defining the research categories reported. The definitions are a list of terms and concepts selected by NIH scientific experts to define a research category. The research category levels represent the NIHs best estimates based on the category definitions ...
Funding levels reported in the Fiscal Year 2016 Intra-Agency Collaborations Reporting System (CRS) may not be consistent with funding levels reported under the Estimates of Funding for Various Research, Condition, and Disease Categories (RCDC) at the NIH Office of Budget Website at https://report.nih.gov/categorical_spending.aspx. The current process, implemented in 2008 through the Research, Condition, and Disease Categorization (RCDC) system, uses sophisticated text data mining (categorizing and clustering using words and multiword phrases) in conjunction with NIH-wide definitions used to match projects to categories. RCDC use of data mining improves consistency and eliminates the wide variability in defining the research categories reported. The definitions are a list of terms and concepts selected by NIH scientific experts to define a research category. The research category levels represent the NIHs best estimates based on the category definitions ...
The American Society for Microbiology (ASM) is the oldest and largest single life science membership organization in the world. Membership has grown from 59 scientists in 1899 to more than 39,000 members today, with more than one third located outside the United States. The members represent 26 disciplines of microbiological specialization plus a division for microbiology educators.
To address whether MyD88 acts in a cell-autonomous fashion, we generated mouse embryonic fibroblasts (MEF) from WT and MyD88-/- mice. MyD88-/- MEFs were resistant to cell transformation by DMBA/TPA in vitro, as assessed by the focus formation assay (Supplemental Figure 2). MyD88-/- MEFs were similarly resistant to transformation after transfection with RasV12/Myc (Figure 1B and Supplemental Figure 2). This resistance was not due to a general defect in transformation, since MyD88-/- MEFs are readily transformed with SV40 T antigen (7), suggesting a selective role for MyD88 in Ras signaling and transformation. We therefore asked whether MyD88 is involved in the canonical Ras/MAPK signaling pathway. While neither p38 nor Akt phosphorylation was affected by absence of MyD88 (Supplemental Figure 3), Erk MAPK phosphorylation was substantially reduced in MyD88-deficient MEFs treated with FGF (Figure 1C). We then retrovirally transduced MyD88-/- MEFs with MyD88. As shown in Supplemental Figure 4, the ...
The DHFR-G8 cell line was produced by M.C. Hung, et al., in 1986 from the NIH/3T3 mouse fibroblast cell line which was cotransfected with the cNEU-p clone and the pSV2-DHFR plasmid. This cell line was developed from one methotrexate resistant colony by selection in 0.6 uM methotrexate and 10% dialyzed calf serum. These cells contain 50-100 copies of the cNeu-P (normal rat cosmid DNA) and produce approximately 4 x 10(5) molecules of encoded p185 protein per cell.
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Site-directed mutagenesis of a synthetic gene coding for low-M(r) phosphotyrosine protein phosphatase from bovine liver has been carried out. The two histidine residues in the enzyme have been mutated to glutamine; both single and double mutants were produced. The mutated and non-mutated sequences have been expressed in Escherichia coli as fusion proteins, in which the low-M(r) phosphotyrosine protein phosphatase was linked to the C-terminal end of the maltose-binding protein. The fusion enzymes were easily purified by single-step affinity chromatography. The mutants were studied for their kinetic properties. Both single mutants showed decreased kcat. values (30 and 7% residual activities for His66 and His72 respectively), and alterations of the Ki values relative to four-competitive inhibitors were observed. The kinetic mechanism of p-nitrophenyl phosphate hydrolysis in the presence of both single mutants was determined and compared with that of the non-mutated enzyme. The rate-determining step ...
Posts about ras-transformed cells were significantly more sensitive to PA than their parental cells ---------------------- profound changes in TUMOR CELL and molecular written by didymusjudasthomas
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An NIH Special Interest Group. From [email protected] Tue Feb 27 14:32:34 2001 Received: from mail.ncifcrf.gov (mail.ncifcrf.gov [129.43.100.100]) by fcs280s.ncifcrf.gov (8.10.0/8.10.0) with ESMTP id f1RJWXA10933; Tue, 27 Feb 2001 14:32:34 -0500 (EST) Received: from gdecker.net.nih.gov (list.nih.gov [165.112.130.6]) by mail.ncifcrf.gov (8.10.1/8.10.1) with ESMTP id f1RJWSN25564; Tue, 27 Feb 2001 14:32:28 -0500 (EST) Received: from list.nih.gov (list.nih.gov [165.112.130.6]) by gdecker.net.nih.gov (8.10.1/8.10.1) with ESMTP id f1RJVi024140; Tue, 27 Feb 2001 14:31:44 -0500 (EST) Received: from LIST.NIH.GOV by LIST.NIH.GOV (LISTSERV-TCP/IP release 1.8d) with spool id 75589 for [email protected]; Tue, 27 Feb 2001 14:31:44 -0500 Received: from helix.nih.gov (helix.nih.gov [128.231.2.3]) by gdecker.net.nih.gov (8.10.1/8.10.1) with ESMTP id f1RJVh024135 for ...
企业荣誉-谱天(天津)生物科技有限公司:谱天所骄傲的合作伙伴a. 蛋白质分析领域:谱天公司与世界顶级蛋白质分析中心有着紧密的合作和业务联系,如美国的NIH、耶鲁医学院、瑞士的苏黎世联邦工学院等著名研究机构,以及美国的Anaspec、德国的JPT等蛋白生物公司。谱天公司还是美国知名蛋白质谱分析专家Balgley博士名下的Bioproximity企业的兄弟公司。谱天的国际合作保证了核心技术与世界同步。临床疾病分析领域:为了获取高质量临床样本进行质谱分析,谱天公司与美国耶鲁大学医学院、杜克大学医学院、
cDNA, FLJ94334, Homo sapiens growth arrest-specific 2 (GAS2), mRNA (Growth arrest-specific 2, isoform CRA_a) contains a PF00307 domain.. cDNA, FLJ94334, Homo sapiens growth arrest-specific 2 (GAS2), mRNA (Growth arrest-specific 2, isoform CRA_a) contains a PF02187 domain.. cDNA, FLJ94334, Homo sapiens growth arrest-specific 2 (GAS2), mRNA (Growth arrest-specific 2, isoform CRA_a) is proteolytically cut by caspase-7 (C14.004) cleavage. SRVD-GKTS.. cDNA, FLJ94334, Homo sapiens growth arrest-specific 2 (GAS2), mRNA (Growth arrest-specific 2, isoform CRA_a) is proteolytically cut by caspase-3 (C14.003) cleavage. SRVD-GKTS.. ...
MDF staff regularly speaks with potential funders and passes the information along to the research community via this space to help increase the breadth and depth of funded research in myotonic dystrophy. In the case of the National Institutes of Health (NIH), annual funding strategies, including pay lines, can be found on the Institute webpages (search "NIAMS," "NINDS," "NHLBI," etc. and "Funding Strategy"). We learned about current funding policies and funding opportunity announcements in a recent meeting with the National Heart, Lung and Blood Institute (NHLBI). Currently, there are very few federally funded projects directed at cardiovascular and respiratory involvement of DM. Because of NIHs focus on funding the best science, increased funding for burdensome heart and respiratory system involvement in DM would require an increase in the number and quality of research grant applications.. Is there an opportunity for DM funding from NHLBI? First, NHLBI is part of the interagency Muscular ...
Anyone could give me some idea where I can find a good protocol for the transformation assay using Rat2 cells. The one you look for transformed foci. Do I need to stain the cells? Alex ...
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The small GTPase RhoA controls many cytoskeletal processes, including actin polymerization, F‐actin bundling, myosin‐based contractility, formation of focal adhesions and formation of the contractile ring at cytokinesis (for a review see Van Aelst and DSouza‐Schorey, 1997). In addition, RhoA plays a less well understood role in the control of cell cycle progression, transformation and tumour cell invasiveness. In fibroblasts, activated RhoA is sufficient to induce DNA synthesis (Olson et al., 1995), and cooperates with activated Raf in focus formation assays (Khosravi‐Far et al., 1995; Qiu et al., 1995). Activated Rho GDP/GTP exchange factors (GEFs) are potent oncogenes in focus assays (for a review see Whitehead et al., 1997), and it has been proposed that their ability to induce anchorage‐independent growth arises because they mimic signalling by adhesion receptors (Schwartz et al., 1996). Rho activity is required for transformation by Ras and for induction of DNA synthesis in ...
Proliferation in cardiac fibroblasts (CFs) can be induced by a wide variety of growth factors that recruit multiple signal transduction pathways, including mitogen-activated protein kinase, phosphatidylinositol 3-kinase and protein kinase C. As a family of dimeric phophoserine-binding proteins, 14-3-3s are associated with a multitude of proteins that regulate signal transduction, apoptosis and checkpoint control pathways. However, it remains unknown whether the 14-3-3 proteins play an active role in cardiac proliferation and alter their expression patterns in response to growth factors in CFs. R18 peptide, an isoform-independent 14-3-3 inhibitor, was used to disrupt 14-3-3 function by adenovirus-mediated transfer of R18-EYFP (AdR18). Our results demonstrate that the 14-3-3 isoforms gamma, zeta and epsilon were highly expressed in CFs and the expression of 14-3-3 epsilon was elevated following serum stimulation. Inhibition of 14-3-3 proteins by AdR18 potentiated mitogen-induced DNA synthesis in ...
TY - JOUR. T1 - Acid-soluble precursors and derivatives of phospholipids increase after stimulation of quiescent swiss 3T3 mouse fibroblasts with serum. AU - Warden, Craig H. AU - Friedkin, M.. AU - Geiger, P. J.. PY - 1980/5/30. Y1 - 1980/5/30. N2 - Automated phosphate analysis of acid-soluble pools of phosphate esters was employed to reveal possible biochemical changes during the transition of Swiss 3T3 mouse fibroblasts from quiescence to active replication of DNA. After 12 hours of stimulation with 10% fetal bovine serum the most notable were 3-fold increases in pools of phospholipid precursors and derivatives. These included glycerophosphocholine, glycerophosphoethanolamine, phosphocholine and phosphoethanolamine. Concurrent but less dramatic increases in pools of ATP, CTP and fructose 1,6-diphosphate were also obtained.. AB - Automated phosphate analysis of acid-soluble pools of phosphate esters was employed to reveal possible biochemical changes during the transition of Swiss 3T3 mouse ...
Cloning, purification, and properties of a phosphotyrosine protein phosphatase from Streptomyces coelicolor A3(2).: We describe the isolation and characterizati
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Tálamus je siva možganovina jajčaste somerne[1] oblike v zadajšnjem delu medmožganov (diencefalona) s številnimi jedri.[2] Pomemben je pri prevajanju senzoričnih in motoričnih signalov v možgansko skorjo[1][3] ter uravnavanju zavesti, spanja in budnosti. Talamus predstavlja del kompleksne strukture možganskih jeder hipotalamusa, epitalamusa, pretalamusa (ventralnega talamusa) in dorzalnega talamusa.[4] ...
Readers will note I do a fair amount of talkin about the ways the NIH-funded research game might be fixed. The NIH has recently posted a request for information on they way they do things. I cant overemphasize how important it is that you comment. Each and every one of you. Whether you are a…