Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.
Four or five slender jointed digits in humans and primates, attached to each HAND.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Inorganic compounds that contain zinc as an integral part of the molecule.
A compound given in the treatment of conditions associated with zinc deficiency such as acrodermatitis enteropathica. Externally, zinc sulfate is used as an astringent in lotions and eye drops. (Reynolds JEF(Ed): Martindale: The Extra Pharmacopoeia (electronic version). Micromedex, Inc, Englewood, CO, 1995)
A family of zinc finger transcription factors that share homology with Kruppel protein, Drosophila. They contain a highly conserved seven amino acid spacer sequence in between their ZINC FINGER MOTIFS.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A mild astringent and topical protectant with some antiseptic action. It is also used in bandages, pastes, ointments, dental cements, and as a sunblock.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Stable zinc atoms that have the same atomic number as the element zinc, but differ in atomic weight. Zn-66-68, and 70 are stable zinc isotopes.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
One of several general transcription factors that are specific for RNA POLYMERASE III. It is a zinc finger (ZINC FINGERS) protein and is required for transcription of 5S ribosomal genes.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A salt produced by the reaction of zinc oxide with acetic acid and used as an astringent, styptic, and emetic.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A zinc-binding domain defined by the sequence Cysteine-X2-Cysteine-X(9-39)-Cysteine-X(l-3)-His-X(2-3)-Cysteine-X2-Cysteine -X(4-48)-Cysteine-X2-Cysteine, where X is any amino acid. The RING finger motif binds two atoms of zinc, with each zinc atom ligated tetrahedrally by either four cysteines or three cysteines and a histidine. The motif also forms into a unitary structure with a central cross-brace region and is found in many proteins that are involved in protein-protein interactions. The acronym RING stands for Really Interesting New Gene.
Proteins that have one or more tightly bound metal ions forming part of their structure. (Dorland, 28th ed)
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Bones that make up the SKELETON of the FINGERS, consisting of two for the THUMB, and three for each of the other fingers.
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
Established cell cultures that have the potential to propagate indefinitely.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A painful disability in the hand affecting the finger or thumb. It is caused by mechanical impingement of the digital flexor tendons as they pass through a narrowed retinacular pulley at the level of the metacarpal head. Thickening of the sheath and fibrocartilaginous metaplasia can occur, and nodules can form. (From Green's Operative Hand Surgery, 5th ed, p2137-58).
An early growth response transcription factor that has been implicated in regulation of CELL PROLIFERATION and APOPTOSIS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Transport proteins that carry specific substances in the blood or across cell membranes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
A ZINC FINGER MOTIF containing transcription factor that was originally identified as one of the IMMEDIATE-EARLY PROTEINS. It shuttles between the CYTOPLASM and the CELL NUCLEUS and is involved in destabilization of mRNAs for TUMOR NECROSIS FACTOR-ALPHA.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Constituent of the 50S subunit of prokaryotic ribosomes containing about 120 nucleotides and 34 proteins. It is also a constituent of the 60S subunit of eukaryotic ribosomes. 5S rRNA is involved in initiation of polypeptide synthesis.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
A low-molecular-weight (approx. 10 kD) protein occurring in the cytoplasm of kidney cortex and liver. It is rich in cysteinyl residues and contains no aromatic amino acids. Metallothionein shows high affinity for bivalent heavy metals.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A transcription factor that plays a role as a key regulator of HEMATOPOIESIS. Aberrant Ikaros expression has been associated with LYMPHOBLASTIC LEUKEMIA.
Isoforms encoded by the WT1 Wilms tumor suppressor gene (GENES, WILMS TUMOR) and produced by alternative splicings. They are zinc finger-containing transcription factors involved in both transactivation and repression, and are critical for normal development and function of the urogenital tract.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Proteins prepared by recombinant DNA technology.
A group of transcription factors that were originally described as being specific to ERYTHROID CELLS.
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
The distal part of the arm beyond the wrist in humans and primates, that includes the palm, fingers, and thumb.
A GATA transcription factor that is expressed in the MYOCARDIUM of developing heart and has been implicated in the differentiation of CARDIAC MYOCYTES. GATA4 is activated by PHOSPHORYLATION and regulates transcription of cardiac-specific genes.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Proteins found in any species of fungus.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.
Proteins encoded by the GAG GENE of the HUMAN IMMUNODEFICIENCY VIRUS.
The act, process, or result of passing from one place or position to another. It differs from LOCOMOTION in that locomotion is restricted to the passing of the whole body from one place to another, while movement encompasses both locomotion but also a change of the position of the whole body or any of its parts. Movement may be used with reference to humans, vertebrate and invertebrate animals, and microorganisms. Differentiate also from MOTOR ACTIVITY, movement associated with behavior.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
A GATA transcription factor that is specifically expressed in hematopoietic lineages and plays an important role in the CELL DIFFERENTIATION of ERYTHROID CELLS and MEGAKARYOCYTES.
Genes at loci that are involved in the development of WILMS TUMOR. Included are human WT1 at 11p13 and human WT2 (MTACR1) at 11p15.
Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream promoter elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.
Proteins that are coded by immediate-early genes, in the absence of de novo protein synthesis. The term was originally used exclusively for viral regulatory proteins that were synthesized just after viral integration into the host cell. It is also used to describe cellular proteins which are synthesized immediately after the resting cell is stimulated by extracellular signals.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
An early growth response transcription factor that controls the formation of the MYELIN SHEATH around peripheral AXONS by SCHWANN CELLS. Mutations in EGR2 transcription factor have been associated with HEREDITARY MOTOR AND SENSORY NEUROPATHIES such as CHARCOT-MARIE-TOOTH DISEASE.
The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.
A continuing periodic change in displacement with respect to a fixed reference. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Force exerted when gripping or grasping.
Deletion of sequences of nucleic acids from the genetic material of an individual.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
A family of transcription factors that are induced by GROWTH FACTORS and contain a highly conserved DNA-binding domain composed of three ZINC FINGER MOTIFS.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
The first digit on the radial side of the hand which in humans lies opposite the other four.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
An idiopathic vascular disorder characterized by bilateral Raynaud phenomenon, the abrupt onset of digital paleness or CYANOSIS in response to cold exposure or stress.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.

A Drosophila doublesex-related gene, terra, is involved in somitogenesis in vertebrates. (1/4650)

The Drosophila doublesex (dsx) gene encodes a transcription factor that mediates sex determination. We describe the characterization of a novel zebrafish zinc-finger gene, terra, which contains a DNA binding domain similar to that of the Drosophila dsx gene. However, unlike dsx, terra is transiently expressed in the presomitic mesoderm and newly formed somites. Expression of terra in presomitic mesoderm is restricted to cells that lack expression of MyoD. In vivo, terra expression is reduced by hedgehog but enhanced by BMP signals. Overexpression of terra induces rapid apoptosis both in vitro and in vivo, suggesting that a tight regulation of terra expression is required during embryogenesis. Terra has both human and mouse homologs and is specifically expressed in mouse somites. Taken together, our findings suggest that terra is a highly conserved protein that plays specific roles in early somitogenesis of vertebrates.  (+info)

ETO-2, a new member of the ETO-family of nuclear proteins. (2/4650)

The t(8;21) is associated with 12-15% of acute myelogenous leukemias of the M2 subtype. The translocation results in the fusion of two genes, AML1 (CBFA2) on chromosome 21 and ETO (MTG8) on chromosome 8. AML1 encodes a DNA binding factor; the ETO protein product is less well characterized, but is thought to be a transcription factor. Here we describe the isolation and characterization of ETO-2, a murine cDNA that encodes a new member of the ETO family of proteins. ETO-2 is 75% identical to murine ETO and shares very high sequence identities over four regions of the protein with ETO (domain I-III and zinc-finger). Northern analysis identifies ETO-2 transcripts in many of the murine tissues analysed and in the developing mouse embryo. ETO-2 is also expressed in myeloid and erythroid cell lines. We confirmed the nuclear localization of ETO-2 and demonstrated that domain III and the zinc-finger region are not required for nuclear localization. We further showed that a region within ETO, containing domain II, mediates dimerization among family members. This region is conserved in the oncoprotein AML-1/ETO. The recent identification of another ETO-like protein, myeloid translocation gene-related protein 1, together with the data presented here, demonstrates that at least three ETO proteins exist with the potential to form dimers in the cell nucleus.  (+info)

Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. (3/4650)

Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex.  (+info)

The mouse Aire gene: comparative genomic sequencing, gene organization, and expression. (4/4650)

Mutations in the human AIRE gene (hAIRE) result in the development of an autoimmune disease named APECED (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy; OMIM 240300). Previously, we have cloned hAIRE and shown that it codes for a putative transcription-associated factor. Here we report the cloning and characterization of Aire, the murine ortholog of hAIRE. Comparative genomic sequencing revealed that the structure of the AIRE gene is highly conserved between human and mouse. The conceptual proteins share 73% homology and feature the same typical functional domains in both species. RT-PCR analysis detected three splice variant isoforms in various mouse tissues, and interestingly one isoform was conserved in human, suggesting potential biological relevance of this product. In situ hybridization on mouse and human histological sections showed that AIRE expression pattern was mainly restricted to a few cells in the thymus, calling for a tissue-specific function of the gene product.  (+info)

Identification and characterization of a zinc finger gene (ZNF213) from 16p13.3. (5/4650)

During our search for the familial Mediterranean fever (FMF) gene, we identified by cDNA selection a 1.2 kb cDNA fragment representing a novel human gene that is expressed in a wide variety of tissues. This gene spans approx. 8.0 kb genomic DNA and has seven exons. Its 3' untranslated region contains a long tandem repeat that gives rise to a polymorphism with two alleles of approx. 1.1 kb and 1.0 kb, with the 1.1 kb allele in strong linkage disequilibrium with FMF in patients of different ethnic backgrounds. However, both genetic and mutational analyses have excluded this gene as the one responsible for FMF. The predicted 424 amino acid protein, designated ZNF213, contains three C2H2 zinc fingers, a Kruppel associated A box and a leucine rich motif (LeR domain/SCAN box), strongly suggestive of a transcription factor.  (+info)

Leukemia translocation protein PLZF inhibits cell growth and expression of cyclin A. (6/4650)

The PLZF gene was identified by its fusion with the RARalpha locus in a therapy resistant form of acute promyelocytic leukemia (APL) associated with the t(11;17)(q23;q21) translocation. Here we describe PLZF as a negative regulator of cell cycle progression ultimately leading to growth suppression. PLZF can bind and repress the cyclin A2 promoter while expression of cyclin A2 reverts the growth suppressed phenotype of myeloid cells expressing PLZF. In contrast RARalpha-PLZF, a fusion protein generated in t(11;17)(q23;q21)-APL activates cyclin A2 transcription and allows expression of cyclin A in anchorage-deprived NIH3T3 cells. Therefore, cyclin A2 is a candidate target gene for PLZF and inhibition of cyclin A expression may contribute to the growth suppressive properties of PLZF. Deregulation of cyclin A2 by RARalpha-PLZF may represent an oncogenic mechanism of this chimeric protein and contribute to the aggressive clinical phenotype of t(11;17)(q23;q21)-associated APL.  (+info)

RFLAT-1: a new zinc finger transcription factor that activates RANTES gene expression in T lymphocytes. (7/4650)

RANTES (Regulated upon Activation, Normal T cell Expressed and Secreted) is a chemoattractant cytokine (chemokine) important in the generation of inflammatory infiltrate and human immunodeficiency virus entry into immune cells. RANTES is expressed late (3-5 days) after activation in T lymphocytes. Using expression cloning, we identified the first "late" T lymphocyte associated transcription factor and named it "RANTES Factor of Late Activated T Lymphocytes-1" (RFLAT-1). RFLAT-1 is a novel, phosphorylated, zinc finger transcription factor that is expressed in T cells 3 days after activation, coincident with RANTES expression. While Rel proteins play the dominant role in RANTES gene expression in fibroblasts, RFLAT-1 is a strong transactivator for RANTES in T cells.  (+info)

Mapping the functional domains of BRCA1. Interaction of the ring finger domains of BRCA1 and BARD1. (8/4650)

Breast cancer 1 (BRCA1) and BRCA1-associated RING domain 1 (BARD1) are multidomain proteins that interact in vivo via their N-terminal RING finger motif regions. To characterize functional aspects of the BRCA1/BARD1 interaction, we have defined the structural domains required for the interaction, as well as their oligomerization state, relative stability, and possible nucleic acid binding activity. We have found that the RING finger motifs do not themselves constitute stable structural domains but are instead part of larger domains comprising residues 1-109 of BRCA1 and residues 26-119 of BARD1. These domains exist as homodimers and preferentially form a stable heterodimer. Shorter BRCA1 RING finger constructs do not interact with BARD1 or with longer BRCA1 constructs, indicating that the heterodimeric and homodimer interactions are mediated by regions outside the canonical RING finger motif. Nucleic acid binding is a generally proposed function of RING finger domains. We show that neither the homodimers nor the heterodimer displays affinity for nucleic acids, indicating that the proposed roles of BRCA1 and BARD1 in DNA repair and/or transcriptional activation must be mediated either by other regions of the proteins or by additional cofactors.  (+info)

TY - JOUR. T1 - Custom DNA-binding proteins come of age. T2 - Polydactyl zinc-finger proteins. AU - Segal, David. AU - Barbas, Carlos F.. PY - 2001/12/1. Y1 - 2001/12/1. N2 - Artificial transcription factors based on modified zinc-finger DNA-binding domains have been shown to activate or repress the transcription of endogenous genes in multiple organisms. Advances in both the construction of novel zinc-finger proteins and our understanding of the characteristics of a productive regulatory site have fueled these achievements.. AB - Artificial transcription factors based on modified zinc-finger DNA-binding domains have been shown to activate or repress the transcription of endogenous genes in multiple organisms. Advances in both the construction of novel zinc-finger proteins and our understanding of the characteristics of a productive regulatory site have fueled these achievements.. UR - UR - ...
Zinc-finger nucleases (ZFNs) are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage domain. Zinc finger domains can be engineered to target specific desired DNA sequences and this enables zinc-finger nucleases to target unique sequences within complex genomes. By taking advantage of endogenous DNA repair machinery, these reagents can be used to precisely alter the genomes of higher organisms. Alongside CRISPR/Cas9 and TALEN, ZFN is a prominent tool in the field of genome editing. The DNA-binding domains of individual ZFNs typically contain between three and six individual zinc finger repeats and can each recognize between 9 and 18 basepairs. If the zinc finger domains perfectly recognize a 3 basepair DNA sequence to generate a 3-finger array that can recognize a 9 basepair target site. Other procedures can utilize either 1-finger or 2-finger modules to generate zinc-finger arrays with six or more individual zinc fingers. The main drawback with ...
Read Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
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A zinc finger motif is an element of proteins that can specifically recognize and bind to DNA. Because they contain multiple cysteine residues, zinc finger motifs possess redox properties. Ionizing radiation generates a variety of free radicals in organisms. Zinc finger motifs, therefore, may be a target of ionizing radiation. The effect of gamma radiation on the zinc finger motifs in transcription factor IIIA (TFIIIA), a zinc finger protein, was investigated. TFIIIA was exposed to different gamma doses from 60Co sources. The dose rates were 0.20 Gy/min and 800 Gy/h, respectively. The binding capacity of zinc finger motifs in TFIIIA was determined using an electrophoretic mobility shift assay. We found that 1000 Gy of gamma radiation impaired the function of the zinc finger... motifs in TFIIIA. The sites of radiation-induced damage in the zinc finger were the thiol groups of cysteine residues and zinc (II) ions. The thiol groups were oxidized to form disulfide bonds and the zinc (II) ions were ...
We have taken a comprehensive approach to the generation of novel DNA binding zinc finger domains of defined specificity. Herein we describe the generation and characterization of a family of zinc finger domains developed for the recognition of each of the 16 possible 3-bp DNA binding sites having the sequence 5-GNN-3. Phage display libraries of zinc finger proteins were created and selected under conditions that favor enrichment of sequence-specific proteins. Zinc finger domains recognizing a number of sequences required refinement by site-directed mutagenesis that was guided by both phage selection data and structural information. In many cases, residues not expected to make base-specific contacts had effects on specificity. A number of these domains demonstrate exquisite specificity and discriminate between sequences that differ by a single base with >100-fold loss in affinity. We conclude that the three helical positions -1, 3, and 6 of a zinc finger domain are insufficient to allow for ...
TY - JOUR. T1 - Differentiation dependent expression in muscle cells of ZT3, a novel zinc finger factor differentially expressed in embryonic and adult tissues. AU - Polimeni, M.. AU - Giorgi, S.. AU - De Gregorio, L.. AU - Dragani, T. A.. AU - Molinaro, M.. AU - Cossu, G.. AU - Bouché, M.. PY - 1996/1. Y1 - 1996/1. N2 - ZT3, isolated from a murine muscle cell cDNA library by a low-stringency hybridization, encodes a zinc finger domain containing factor with a transcript of 5.0 kb. A 3 2.5 kb partial nucleotide sequence contains an ORF of 1.5 kb where 17 canonical C2H2 zinc finger domains organized in tandem were identified. It maps on mouse chromosome 11, close to two mutations which affect skeletal formation. ZT3 expression depends upon differentiation of myogenic cells in culture, since it is upregulated with myogenin and inhibited in scr-transfected C2C12 cells. ZT3 is not expressed in NIH3T3 or C3H10T1/2 fibroblasts, but is induced when fibroblasts are myogenically converted by ...
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The corpus luteum (CL) is a temporary organ involved in the maintenance of pregnancy. In the course of its life-cycle, the CL undergoes two distinct and consecutive processes for its inevitable removal through apoptosis: functional and structural luteolysis. We isolated a gene encoding for a novel rat zinc finger protein (ZFP), named rat ZFP96 (rZFP96) from an ovarian lambda cDNA library. Sequence analysis revealed close sequence and structural similarity to mouse ZFP96 and human zinc finger protein 305 (ZNF305). Quantitative reverse transcription-polymerase chain reaction analysis revealed a positive correlation with the end of pregnancy, that is, the onset of structural luteolysis of the CL. Messenger RNA levels increased 3-fold (P < 0.01) between days 13 and 22 of pregnancy and 8-fold (P < 0.01) between day 13 of pregnancy and day 1 post-partum. In addition, we detected rZFP96 expression in mammary, placenta, heart, kidney and skeletal muscle. Sequence analysis predicted that rZFP96 has a ...
Zinc finger nucleases (ZFNs) are proteins composed of a DNA binding-domain and a DNA cleaving-domain. One zinc finger binding domain recognizes and binds to a three nucleotide sequence, one can thus increase the binding specificity of a ZFN by adding more zinc fingers. The DNA cleaving-domain (Fok 1 nuclease) can then be used to create a double-stranded break in DNA at the desired point (Fig. 1). After a double stranded break, DNA is repaired by non-homologous end joining. This often causes small insertions or deletions in the DNA, resulting frame-shift mutations. These frame-shifts result in nonsense mutations or nonsense-mediated decay. These effects are therefore very useful for creating knockout genes. Additionally, genetic material such as DNA plasmids can be integrated into a gene after a double stranded break. A desired donor plasmid will contain arms of DNA which are homologous to the ZFN cut site. The donor plasmid and ZFNs can then be added simultaneously to the cell, the genetic ...
Zinc finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc finger proteins contain a Krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zinc finger protein 75 (ZNF75), also known as ZNF82, is a 289 amino acid member of the Krueppel C2H2-type zinc finger protein family. Localized to the nucleus, ZNF75 contains five C2H2- type zinc fingers and one KRAB domain through which it is thought to be involved in DNA-binding and transcriptional regulation ...
Zinc finger inhibitors, or zinc ejectors, are substances or compounds that interact adversely with zinc fingers and cause them to release their zinc from its binding site, disrupting the conformation of the polypeptide chain and rendering the zinc fingers ineffective, thereby preventing them from performing their associated cellular functions. This is typically accomplished through chelation of the zinc binding site. As zinc fingers are known to be involved in m-RNA regulation, reverse transcription, protection of synthesized viral DNA, transcription inhibition, and initial integration processes, prevention of zinc finger function can have drastic effects on the function of the cell or virus. Zinc finger inhibitors are typically used to combat HIV. HIV treatments usually rely on targeting reverse transcriptases and proteases. However, these methods are proving to be ineffective due to the development of resistant strains of the virus or due to the stoppage of the treatment. This method of using ...
Artificial transcription factors based on modified zinc-finger DNA-binding domains have been shown to activate or repress the transcription of endogenous genes in multiple organisms. Advances in both the construction of novel zinc-finger proteins and our understanding of the characteristics of a productive regulatory site have fueled these achievements ...
Zinc finger nucleases (ZFNs) are associated with cell death and apoptosis by binding at countless undesired locations. This cytotoxicity is associated with the binding ability of engineered zinc finger domains to bind dissimilar DNA sequences with high affinity. In general, binding preferences of transcription factors are associated with significant degenerated diversity and complexity which convolutes the design and engineering of precise DNA binding domains. Evolutionary success of natural zinc finger proteins, however, evinces that nature created specific evolutionary traits and strategies, such as modularity and rank-specific recognition to cope with binding complexity that are critical for creating clinical viable tools to precisely modify the human genome. Our findings indicate preservation of general modularity and significant alteration of the rank-specific binding preferences of the three-finger binding domain of transcription factor SP1 when exchanging amino acids in the 2nd finger. ...
BACKGROUND ZNF32 has been predicted to be a zinc finger protein and is involved in cell differentiation and tumor development, but its precise function is unknown. Specific monoclonal antibodies (mAbs) have been widely used in research and clinical diagnosis and treatments. Therefore, we established an anti-ZNF32 mAb to characterize this proteins function. MATERIAL/METHODS Peptide⁴⁹⁻⁶³, a specific small peptide of ZNF32, was chosen and the synthetic keyhole limpet hemocyanin (KLH)-peptide⁴⁹⁻⁶³ was used as an antigen to immunize mice. A mAb against peptide⁴⁹⁻⁶³ was generated by hybridoma technology, and hybridoma cells were screened by limiting dilution. The isoform of mAb-pZNF32-8D9 was identified by double agar diffusion. The sensitivity and specificity of the mAb and expressed levels of ZNF32 in various cells and tissues were identified by enzyme-linked immunosorbent assay (ELISA), immunocytochemistry, immunohistochemistry, and Western blotting. RESULTS A stable anti
Plasmids for genome engineering using zinc finger nucleases (ZFN) constructed via modular assembly of artificial zinc finger arrays.
We report here an efficient method for targeted mutagenesis of Arabidopsis genes through regulated expression of zinc finger nucleases (ZFNs)-enzymes engineered to create DNA double-strand breaks at specific target loci. ZFNs recognizing the Arabidopsis ADH1 and TT4 genes were made by Oligomerized Pool ENgineering (OPEN)-a publicly available, selection-based platform that yields high quality zinc finger arrays. The ADH1 and TT4 ZFNs were placed under control of an estrogen-inducible promoter and introduced into Arabidopsis plants by floral-dip transformation. Primary transgenic Arabidopsis seedlings induced to express the ADH1 or TT4 ZFNs exhibited somatic mutation frequencies of 7% or 16%, respectively. The induced mutations were typically insertions or deletions (1-142 bp) that were localized at the ZFN cleavage site and likely derived from imprecise repair of chromosome breaks by nonhomologous end-joining. Mutations were transmitted to the next generation for 69% of primary transgenics expressing the
The maize INDETERMINATE1 gene, ID1, is a key regulator of the transition to flowering and the founding member of a transcription factor gene family that encodes a protein with a distinct arrangement of zinc finger motifs. The zinc fingers and surrounding sequence make up the signature ID domain (IDD), which appears to be found in all higher plant genomes. The presence of zinc finger domains and previous biochemical studies showing that ID1 binds to DNA suggests that members of this gene family are involved in transcriptional regulation. Comparison of IDD genes identified in Arabidopsis and rice genomes, and all IDD genes discovered in maize EST and genomic databases, suggest that ID1 is a unique member of this gene family. High levels of sequence similarity amongst all IDD genes from maize, rice and Arabidopsis suggest that they are derived from a common ancestor. Several unique features of ID1 suggest that it is a divergent member of the maize IDD family. Although no clear ID1 ortholog was identified
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Zinc finger proteins (ZNFs) bind DNA and, through this binding, regulate gene transcription. Most ZNFs contain conserved C2H2 motifs and are classified as Kruppel-type zinc fingers. For a general description of these proteins, see ZNF91 (MIM 603971).[supplied by OMIM, Jul 2002]
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Blimp1, a zinc-finger containing DNA-binding transcriptional repressor, functions as a master regulator of B cell terminal differentiation. Considerable evidence suggests that Blimp1 is required for the establishment of anteroposterior axis formation and the formation of head structures during early vertebrate development. In mouse embryos, Blimp1 is strongly expressed in axial mesendoderm, the tissue known to provide anterior patterning signals during gastrulation. Here, we describe for the first time the defects caused by loss of Blimp1 function in the mouse. Blimp1 deficient embryos die at mid-gestation, but surprisingly early axis formation, anterior patterning and neural crest formation proceed normally. Rather, loss of Blimp1 expression disrupts morphogenesis of the caudal branchial arches and leads to a failure to correctly elaborate the labyrinthine layer of the placenta. Blimp1 mutant embryos also show widespread blood leakage and tissue apoptosis, and, strikingly, Blimp1 homozygous mutants
TY - JOUR. T1 - An internal deletion within an 11p13 zinc finger gene contributes to the development of Wilms tumor. AU - Haber, Daniel A.. AU - Buckler, Alan J.. AU - Glaser, Thomas M. AU - Call, Katherine M.. AU - Pelletier, Jerry. AU - Sohn, Robert L.. AU - Douglass, Edwin C.. AU - Housman, David E.. PY - 1990/6/29. Y1 - 1990/6/29. N2 - We have recently described the isolation of a candidate for the Wilms tumor susceptibility gene mapping to band p13 of human chromosome 11. This gene, primarily expressed in fetal kidney, appears to encode a DNA binding protein. We now describe a sporadic, unilateral Wilms tumor in which one allele of this gene contains a 25 bp deletion spanning an exon-intron junction and leading to aberrant mRNA splicing and loss of one of the four zinc finger consensus domains in the protein. The mutation is absent in the affected individuals germline, consistent with the somatic inactivation of a tumor suppressor gene. In addition to this intragenic deletion affecting ...
We have previously reported (Villa et al. (1993), Genomics 18: 223) the characterization of the human ZNF75 gene located on Xq26, which has only limited homology (less than 65%) to other ZF genes in the databases. Here, we describe three human zinc finger genes with 86 to 95% homology to ZNF75 at the nucleotide level, which represent all the members of the human ZNF75 subfamily. One of these, ZNF75B, is a pseudogene mapped to chromosome 12q13. The other two, ZNF75A and ZNF75C, maintain an ORF in the sequenced region, and at least the latter is expressed in the U937 cell line. They were mapped to chromosomes 16 and 11, respectively. All these genes are conserved in chimpanzees, gorillas, and orangutans. The ZNF75B homologue is a pseudogene in all three great apes, and in chimpanzee it is located on chromosome 10 (phylogenetic XII), at p13 (corresponding to the human 12q13). The chimpanzee homologue of ZNF75 is also located on the Xq26 chromosome, in the same region, as detected by in situ ...
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An inducible program of inflammatory gene expression is a hallmark of antimicrobial defenses. This response is controlled by a collaboration involving signal-dependent activation of transcription factors, transcriptional co-regulators, and chromatin-modifying factors. Here we have identified a highly conserved Zinc finger DNA binding protein CNBP (also called ZNF9) upregulated in myeloid cells exposed to lipopolysaccharide. CNBP resides primarily in the cytosol and upon TLR4 engagement, CNBP translocate to the nucleus. To investigate the functional consequences of these events, we generated mice lacking CNBP and characterized the role of CNBP in controlling the inducible transcriptional program using a combination of RNA-sequencing and multiplex gene expression analysis (Nanostring). In response to an array of signals such as LPS, CNBP-deficient macrophages were impaired in their ability to induce important immune genes including IL12p40 and IL6 amongst others. CNBP-deficient cells showed normal ...
EntrezGene ,Full_name_from_nomenclature_authority=CCCTC-binding factor (zinc finger protein) ,GeneID=10664 ,LocusTag=- ,Modification_date=20120108 ,Nomenclature_status=O ,Other_designations=11 zinc finger transcriptional repressor;;11-zinc finger protein;;CTCFL paralog;;transcriptional repressor CTCF ,Symbol=CTCF ,Symbol_from_nomenclature_authority=CTCF ,Synonyms=- ,chromosome=16 ,dbXrefs=HGNC:13723;;MIM:604167;;Ensembl:ENSG00000102974;;HPRD:05005;;Vega:OTTHUMG00000137539;;EpiFactors:10664:genes ,description=CCCTC-binding factor (zinc finger protein) ,map_location=16q21-q22.3 ,tax_id=9606 ,tf?=yes ,transcription_factor= ,type_of_gene=protein-coding ...
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Neale, Matt (2010) PRDM9 points the zinc finger at meiotic recombination hotspots. Genome Biology, 11 (2). p. 104. ISSN 14656914 Full text not available from this repository ...
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Click to launch & play an online audio visual presentation by Prof. Carlos Barbas on Polydactyl zinc finger proteins: software and hardware for genomes, part of a collection of multimedia lectures.
The H3K9me3 histone modification is often found at promoter regions, where it functions to repress transcription. However, we have previously shown that 3′ exons of zinc finger genes (ZNFs) are marked by high levels of H3K9me3. We have now further investigated this unusual location for H3K9me3 in ZNF genes. Neither bioinformatic nor experimental approaches support the hypothesis that the 3′ exons of ZNFs are promoters. We further characterized the histone modifications at the 3′ ZNF exons and found that these regions also contain H3K36me3, a mark of transcriptional elongation. A genome-wide analysis of ChIP-seq data revealed that ZNFs constitute the majority of genes that have high levels of both H3K9me3 and H3K36me3. These results suggested the possibility that the ZNF genes may be imprinted, with one allele transcribed and one allele repressed. To test the hypothesis that the contradictory modifications are due to imprinting, we used a SNP analysis of RNA-seq data to demonstrate that both
Zinc finger CCHC domain-containing protein 8 contains a PF00098 domain.. Zinc finger CCHC domain-containing protein 8 contains a PF04046 domain.. Zinc finger CCHC domain-containing protein 8 is proteolytically cut by granzyme B, human-type (S01.010) cleavage. MDED-ALTL.. ...
TY - JOUR. T1 - Egr3/Pilot, a zinc finger transcription factor, is rapidly regulated by activity in brain neurons and colocalizes with Egr1/zif268. AU - Yamagata, K.. AU - Kaufmann, W. E.. AU - Lanahan, A.. AU - Papapavlou, M.. AU - Barnes, Carol A. AU - Andreasson, K. I.. AU - Worley, P. F.. PY - 1994/7. Y1 - 1994/7. UR - UR - M3 - Article. C2 - 10467592. AN - SCOPUS:0028466211. VL - 1. SP - 140. EP - 152. JO - Learning and Memory. JF - Learning and Memory. SN - 1072-0502. IS - 2. ER - ...
TY - JOUR. T1 - Zinc finger protein 131 inhibits estrogen signaling by suppressing estrogen receptor α homo-dimerization. AU - Oh, Yohan. AU - Chung, Kwang Chul. PY - 2013/1/4. Y1 - 2013/1/4. N2 - Steroid hormone estrogen elicits various physiological functions, many of which are mediated through two structurally and functionally distinct estrogen receptors, ERα and ERβ. The functional role of zinc finger protein 131 (ZNF131) is poorly understood, but it is assumed to possess transcriptional regulation activity due to the presence of a DNA binding motif. A few recent reports, including ours, revealed that ZNF131 acts as a negative regulator of ERα and that SUMO modification potentiates the negative effect of ZNF131 on estrogen signaling. However, its molecular mechanism for ERα inhibition has not been elucidated in detail. Here, we demonstrate that ZNF131 directly interacts with ERα, which consequently inhibits ERα-mediated trans-activation by suppressing its homo-dimerization. Moreover, ...
TY - JOUR. T1 - Testicular zinc finger protein recruits histone deacetylase 2 and suppresses the transactivation function and intranuclear foci formation of agonist-bound androgen receptor competitively with TIF2. AU - Tao, Rong Hua. AU - Kawate, Hisaya. AU - Wu, Yin. AU - Ohnaka, Keizo. AU - Ishizuka, Masamichi. AU - Inoue, Atsuto. AU - Hagiwara, Hiromi. AU - Takayanagi, Ryoichi. PY - 2006/3/9. Y1 - 2006/3/9. N2 - We previously reported that testicular zinc finger protein (TZF) is a corepressor for androgen receptor (AR). The present study demonstrated that a central portion (amino acids 512-663) of TZF, TZF(512-663), is responsible for both binding to AR and repressing the transactivation. TZF recruited endogenous histone deacetylase 2 (HDAC2) and formed a complex with agonist-bound AR. Imaging analyses showed that TZF and TZF(512-663) were recruited by AR and simultaneously impaired distinct AR foci formation. Quantification of the foci number using a three-dimensional imaging method revealed ...
TY - JOUR. T1 - Zinc finger protein 28 as a novel melanoma-related molecule. AU - Yajima, Ichiro. AU - Kumasaka, Mayuko. AU - Thang, Nguyen Dinh. AU - Yanagishita, Takeshi. AU - Ohgami, Nobutaka. AU - Kallenberg, David. AU - Naito, Yuji. AU - Yoshikawa, Toshikazu. AU - Sakashita, Naomi. AU - Kato, Masashi. PY - 2009/7/1. Y1 - 2009/7/1. KW - Cancer. KW - Melanocyte. KW - Melanoma. KW - Zinc finger protein. KW - c-Ret. UR - UR - U2 - 10.1016/j.jdermsci.2009.02.010. DO - 10.1016/j.jdermsci.2009.02.010. M3 - Letter. C2 - 19329283. AN - SCOPUS:67349128973. VL - 55. SP - 68. EP - 70. JO - Journal of Dermatological Science. JF - Journal of Dermatological Science. SN - 0923-1811. IS - 1. ER - ...
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Artificial transcription factors can be engineered to interact with specific DNA sequences to modulate endogenous gene expression within cells. A significant hurdle to implementation of this approach is the selection of the appropriate DNA sequence for targeting. We reasoned that a good target site should be located in chromatin, where it is accessible to DNA-binding proteins, and it should be, in the close vicinity of known transcriptional regulators of the gene. Here we have explored the efficacy of these criteria to guide our selection of potential regulators of gamma-globin expression. Several zinc finger-based transcriptional activators were designed to target the sites proximal to the -117-position of the gamma-globin promoter. This region is proximal to the binding sites of known and potential natural transcription factors. Design and study of three transcription factors identified the potent transcriptional activator, ggl-VP64-RA. This transcription factor was able to interact directly ...
Sangamo BioSciences, Inc. is focused on research and development of novel DNA-binding proteins for therapeutic gene regulation and genome editing. The Company has ongoing Phase 2 clinical trials to evaluate the safety and efficacy of a novel ZFP Therapeutic® for the treatment of HIV/AIDS. Sangamos other therapeutic programs are focused on monogenic diseases, including hemophilia, Huntingtons disease and hemoglobinopathies such as sickle cell anemia and beta-thalassemia. Sangamos core competencies enable the engineering of a class of DNA-binding proteins known as zinc finger DNA-binding proteins (ZFPs). Engineering of ZFPs that recognize a specific DNA sequence enables the creation of sequence-specific ZFP Nucleases (ZFNs) for gene modification and ZFP transcription factors (ZFP TFs) that can control gene expression and, consequently, cell function. Sangamo has entered into a strategic collaboration with Shire AG to develop therapeutics for hemophilia, Huntingtons disease and other monogenic ...
Direct editing of disease-causing mutations has obvious attractions for the treatment of genetic disorders if the many practical obstacles to the technique can be overcome. One promising line of research centres on the development of zinc finger nucleases (ZFNs) produced by fusing an engineered zinc finger DNA-binding domain to an endonuclease. These artificial enzymes induce efficient gene correction in cultured cells. Li et al. now report that zinc finger nucleases induce double-strand breaks in specifically selected locations on the genome and stimulate genome editing at a clinically meaningful level in vivo. In a proof-of-principle experiment, ZFNs delivered to the liver in a mouse model of haemophilia B achieved a level of gene replacement that was sufficient to correct the clotting defect, and the effect persisted following liver regeneration. Editing of the human genome to correct disease-causing mutations is a promising approach for the treatment of genetic disorders. Genome editing improves on
TY - JOUR. T1 - Outlier analysis defines zinc finger gene family DNA methylation in tumors and saliva of head and neck cancer patients. AU - Gaykalova, Daria A.. AU - Vatapalli, Rajita. AU - Wei, Yingying. AU - Tsai, Hua Ling. AU - Wang, Hao. AU - Zhang, Chi. AU - Hennessey, Patrick T.. AU - Guo, Theresa. AU - Tan, Marietta. AU - Li, Ryan. AU - Ahn, Julie. AU - Khan, Zubair. AU - Westra, William H.. AU - Bishop, Justin A.. AU - Zaboli, David. AU - Koch, Wayne M.. AU - Khan, Tanbir. AU - Ochs, Michael F.. AU - Califano, Joseph A.. N1 - Funding Information: The analysis in this article is based on a web database applications provided by Research Information Technology Systems (RITS)- This work was supported by the National Institute of Dental and Craniofacial Research and National Institute of Health Challenge Grant (RC1DE020324); National Institute of Dental and Craniofacial Research and National Cancer Institute grant (P50 DE 019032 Head and Neck Cancer SPORE) to ...
Spodoptera frugiperda SF-21 cells infected with Autographa californica nuclear polyhedrosis virus mutants which lack a functional p35 gene undergo apoptosis, a type of programmed cell death. To identify p35-homologous genes in other baculoviruses, A. californica nuclear polyhedrosis virus DNA containing a deletion in p35 was cotransfected into SF-21 cells along with genomic DNAs from other baculoviruses. One of the viral DNAs which were able to rescue wild-type infection was from Cydia pomonella granulosis virus (CpGV). The CpGV gene responsible for the effect was mapped to a 1.6-kb SalI-SstI subclone of the SalI B fragment of CpGV. The sequence of the SalI-SstI subclone revealed an open reading frame capable of encoding a polypeptide of 31 kDa which was sufficient to rescue wild-type infection; this gene was thus called iap (inhibitor of apoptosis). The predicted sequence of the IAP polypeptide exhibited no significant homology to P35 but contained a zinc finger-like motif which is also found ...
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Nucleases are the enzyme, used to cleave DNA into smaller units. Zinc-finger (ZFN) nucleases are artificial restriction enzyme used to cleave DNA into smaller fragments. It is the class of engineered DNA-binding proteins that creates double standard break at specified locations. It consist of two functional domain, a DNA-binding domain, and a DNA-cleaving domain. DNA binding domain recognizes the unique hexamer sequence of DNA and DNA-cleaving domain consisting nuclease domain of Fok I. The fusion between the DNA-binding domain, and a DNA-cleaving domain creates artificial restriction enzyme known as molecular scissor that cleaves the desired DNA sequence. ZFN is based on the DNA repair machinery and is becoming a prominent tool in the field of genome editing.. Zinc finger nucleases are useful for various biotechnological and life science applications. It is used to manipulate plants and animals for research purpose and is used in the clinical trial of CD4+ human T-cells for the treatment of ...
The first three zinc fingers (ZF1-3) of transcription factor IIIA (TFIIIA) from Xenopus have been shown to contribute the majority of the binding energy to the intact TFIIIA-DNA interaction [Liao et al. (1992) J. Mel. Biol, 223, 857-871]. We have expressed a 92-amino acid polypeptide containing the three N-terminal zinc fingers of TFIIIA. This three-fingered polypeptide has
CCCH zinc finger alignments in Postia placenta . Alignments can be refined by adding alignments from other genomes, adding your own sequences and/or aligning to other models from the same superfamily. The display of alignments can also be customised.
Krüppel-like factor (KLF) family members share a three C2H2 zinc finger DNA binding domain, and are involved in cell proliferation and differentiation control in normal as in pathological situations. KLFs can be deregulated in multiple cancers either by loss of heterozygosity (LOH), somatic mutation or transcriptional silencing by promoter hypermethylation. KLF family member proteins play a critical role in the growth and metastasis of numerous tumor types, at least in part by regulating the expression of cell cycle genes. Globally, KLF4 and KLF6 are considered as tumor suppressor gene, whereas KLF5 promotes cell proliferation. Family members have different transcriptional properties and can modulate each others activity by a variety of mechanisms. Since cells can express multiple KLFs, KLF transcription factors build likely a transcriptional network to control cell proliferation. Effects of changes in KLF factors are context-dependent and can appear contradictory, considering differences in ...
From NCBI Gene:. In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5 exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5 exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]. From UniProt: ...
GATA-1 and FOG-1 zinc fingers. Computer model showing the complex of the zinc finger transcription factors GATA-1 (orange) and FOG-1 (friend of GATA, blue). The two zinc ions shown in grey become visible after removing a few atoms hiding them. - Stock Image C035/5609
Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). Associates with the transcriptional repressor ZNF683 to chromatin at gene ...
Genetic analysis and homology between the phenotypic alterations of the human Greig Cephalopolysyndactyly Syndrome (GCPS) and the mouse mutant extra-toes (Xt) have suggested a dominant mutation in the same gene of both species. Recently, the GLI3 gene, a member of the Kruppel-related zinc finger genes, has been proposed as a candidate gene for GCPS. We examined the expression of the mouse Gli3 gene in both Xt mutant animals and during normal mouse development. Northern and RNAase protection analysis of embryos revealed that Gli3 expression was reduced about 50% in heterozygous Xt/+ mice and completely absent in homozygous Xt/Xt mice. In addition, in situ analysis of wild-type mice documented Gli3 expression in the developing limb and brain, structures affected in Xt mutant mice. This pattern suggests an important function of the Gli3 gene during morphogenesis.. ...
National Cancer Institute. Evi3 is a common site of retroviral integration in B-cell lymphomas of AKXD mice. BLAST searches of Evi3 genomic sequences against the mouse and human databases showed that most viral integrations at Evi3 are located immediately upstream of the first translated exon (exon 2) of a gene encoding a novel zinc finger protein with 30 kr ppel-like zinc finger repeats. Viral integrations at Evi3 upregulate the expression of this gene via promoter sequences present in the viral long terminal repeat. EVI3 protein is highly related to the early B-cell associated zinc finger protein EBFAZ, and all 30 zinc fingers found in EVI3 are conserved in EBFAZ. Ebfaz and Evi3 are coexpressed in many cell types, and EVI3, like EBFAZ, is located in the nucleus. EBFAZ binds to, and negatively regulates, early B cell factor (EBF), a basic helix-loop-helix transcription factor required for B-cell-lineage commitment. EBFAZ also binds to SMAD1 and SMAD4 in response to BMP2 signaling, which in turn ...
Education. -1998 PhD (4 years program) at University of Rome La Sapienza in Human Biology: Cellular and Molecular Biology Bases. -1991 Degree in Biology, University of Rome La Sapienza Department of Human Biopathology. University of Rome La Sapienza, Rome, Italy. Experimental Thesis, summa cum laude.. Current position and research experience. -From 2009 to present CNR Researcher (III level) at Istituto Biologia e Patologia Molecolari (IBPM), CNR, Rome, Italy.. -From 1999 to 2008 post-doctoral fellow at CNR, Istituto Biologia e Patologia Molecolari (IBPM), Rome, Italy.. -From 1994 to1998 PhD (4 years program) at University of Rome La Sapienza in Human Biology: Cellular and Molecular Biology Bases. Thesis on artificial zinc finger transcription factors programmed to upregulate utrophin gene in Duchenne Muscular Dystrophy (DMD). Supervisor: Dr. Claudio Passananti.. -From 1995 to 1993 research experience as PhD/fellow in the field of Regulation of gene expression and development in the ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
In order to study the mechanism of neural patterning in Xenopus, we used subtractive cloning to isolate genes activated early during this process. One gene isolated was opl, (odd-paired-like) that resembles the Drosophila pair-rule gene odd-paired and encodes a zinc finger protein that is a member of the Zic gene family. At the onset of gastrulation, opl is expressed throughout the presumptive neural plate, indicating that neural determination has begun at this stage while, by neurula, opl expression is restricted to the dorsal neural tube and neural crest. opl encodes a transcriptional activator, with a carboxy terminal regulatory domain, which when removed increases opl activity. opl both sensitizes animal cap ectoderm to the neural inducer noggin and alters the spectrum of genes induced by noggin, allowing activation of the midbrain marker engrailed. Consistent with the later dorsal neural expression of opl, the activated form of opl is able to induce neural crest and dorsal neural tube ...
Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA. The epithelium lining the intestine undergoes rapid and continuous renewal. Growth factors play a role in intestinal epithelial growth regulation in vitro and in vivo. In this study, transforming growth factor alpha (TGF alpha) is shown to act as a mitogen and induce the expression of two zinc finger-containing immediate early genes [Zif268 (zinc finger protein 268) and Nup475 (nuclear protein 475)] in rat intestinal epithelial (RIE-1) cells in culture. These two gene products were initially isolated from serum-treated fibroblasts and represent growth-stimulated transcription factors. In TGF alpha-treated RIE-1 cells, nuclear run-on experiments demonstrate that TGF alpha induction of these two genes is regulated predominantly at the level of gene transcription. Utilizing in situ hybridization techniques, we show that systemic administration of TGF alpha induces expression of these two genes in the rat ...
Exploring the Recognition of Quadruplex DNA by an Engineered Cys2-His2 Zinc Finger Protein. Biochemistry 45 (5) , pp. 1393-1399. 10.1021/bi050229x ...
1CS3: Structure-function studies of the BTB/POZ transcriptional repression domain from the promyelocytic leukemia zinc finger oncoprotein.
The ubiquitin-binding zinc finger (UBZ) is a type of zinc-coordinating β-β-α fold domain found mainly in proteins involved in DNA repair and transcriptional regulation. UBZ domains coordinate a zinc ion with cysteine or histidine residues; depending on their amino acid sequence, UBZ domains are classified into several families [1,2]. Type 1 UBZs are CCHH-type zinc fingers found in tandem UBZ domains of TAX1-binding protein 1 (TAX1BP1) [3,4,5], type 2 UBZs are CCHC-type zinc fingers found in FAAP20 which is a subunit of the Fanconi anemia (FA) core complex [6,7], type 3 UBZs are CCHH-type zinc fingers found only in the Y-family translesion polymerase eta [8,9,10], and type 4 UBZs are CCHC-type zinc fingers found in Y-family translesion polymerase kappa, Werner helicase-interacting protein 1 (WRNIP1), and Rad18 [11,12,13]. The UBZ domain consists of two short antiparallel β-strands followed by one α-helix. The α-helix packs against the β-strands with a zinc ion sandwiched between the ...
Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases - Disclosed herein are methods and compositions for inactivating TCR genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided. Disclosed herein are also methods and compositions for expressing a functional exogenous TCR in the absence of endogenous TCR expression in T lymphocytes, including lymphocytes with a central memory phenotype. Polynucleotides encoding exogenous TCR, vectors comprising polynucleotides encoding exogenous TCR and cells comprising polynucleotides encoding exogenous TCR and/or cells comprising exogenous TCR are also provided ...
Zinc is an essential trace element that plays a crucial role in catalytic, structural and regulatory functions of many proteins including enzymes and transcription factors; thus maintenance of zinc balance is critical for normal cellular function. Cellular mechanisms that maintain zinc balance include the regulation of genes coding for proteins that play vital roles in zinc homeostasis. These proteins include zinc transporters belonging to the ZIP (SLC39A) and ZnT (SLC30A) families as well as the zinc-binding metallothionein proteins. In contrast to bacterial and yeast systems, a transcription factor responsible for mediating transcriptional repression of a suite of genes in response to elevated zinc levels in mammals has hitherto not been identified. Using Caco-2 cells as a model of human intestinal epithelial cells and detection of protein binding by electrophoretic mobility shift analysis, we show that zinc finger protein ZNF658 binds specifically to the zinc transcriptional regulatory ...
Inhibitor of apoptosis proteins (IAPs) constitute a conserved family of molecules, which regulate both apoptosis and receptor signalling. They are often deregulated in cancer cells and represent potential targets for therapy. In my work, I investigated the effect of IAP inhibition in vivo to identify novel down-stream genes expressed in an IAP-dependent manner that could contribute to cancer aggressiveness. To this end, immunocompromised mice engrafted subcutaneously with the triple negative breast cancer (TNBC) cell line MDA-MB231 were treated with SM83, a Smac mimetic developed in our laboratory that acts as a pan-IAP inhibitor, and tumour nodules were profiled for gene expression. The analysis revealed that the inhibition of IAPs significantly reduces the expression of SNAI2, a zinc finger transcriptional repressor often associated with cancer aggressiveness, resistance to therapy and metastatic potential, especially in breast cancer. By testing several TNBC cell lines, I found that SNAI2 ...
Krüppel-like factor 5 (intestinal) or Krüppel-like factor 5 (KLF5) is a zinc finger-containing transcription factor and involved in important biological processes including cell proliferation and differentiation. However, clinical significance of KLF5 protein has remained largely unknown in breast cancer. Therefore, in this study, we immunolocalized KLF5 in 113 human breast carcinoma cases. KLF5 immunoreactivity was frequently detected in the nuclei of breast carcinoma cells, and median value of the ratio of KLF5-positive carcinoma cells was 30% and was positively associated with the status of androgen receptor. KLF5 immunoreactivity was also significantly associated with increased risk of recurrence and worse clinical outcome in breast cancer patients by univariate analyses, and subsequent multivariate analyses demonstrated that KLF5 immunoreactivity was an independent prognostic factor for both disease-free and breast cancer-specific survival of the patients. We then examined possible regulation of
TY - JOUR. T1 - Finger inter-dependence. T2 - Linking the kinetic and kinematic variables. AU - Kim, Sun Wook. AU - Shim, Jae Kun. AU - Zatsiorsky, Vladimir M.. AU - Latash, Mark L.. N1 - Funding Information: This study was supported in part by NIH Grants AG-018751, AR-048563, and NS-35032.. PY - 2008/6. Y1 - 2008/6. N2 - We studied the dependence between voluntary motion of a finger and pressing forces produced by the tips of other fingers of the hand. Participants moved one of the fingers (task finger) of the right hand trying to follow a cyclic, ramp-like flexion-extension template at different frequencies. The other fingers (slave fingers) were restricted from moving; their flexion forces were recorded and analyzed. Index finger motion caused the smallest force production by the slave fingers. Larger forces were produced by the neighbors of the task finger; these forces showed strong modulation over the range of motion of the task finger. The enslaved forces were higher during the flexion ...
Each competitor and mentor would love to have something that would avoid finger wounds. A torn tendon can prompt to lessen playing time for a competitor. There is additionally the danger of lasting handicap. These conceivable outcomes cause numerous competitors to wear a finger prop practically speaking and even in amusements. Concentrates on have demonstrated that the prophylactic finger support can forestall harm yet there are clashing reports also. The finger is uncovered and exceptionally helpless amid athletic action and in view of its huge size it is frequently harmed. In sports, finger supports have two purposes. They are intended to ensure the competitor with past finger wounds. The finger prop underpins the finger to diminish torment. It likewise keeps the finger from being harmed again as it mends. The prop additionally ensures the finger amid substantial physical games and keeps the finger from being harmed. Browse around this website ...
Dergai, O., Cousin, P., Gouge, J., Satia, K., Praz, V., Kuhlman, T., Lhote, P., Vannini, A., Hernandez, N. (May 2018) Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters. Genes Dev, 32 (9-10). pp. 711-722. ISSN 0890-9369 Denissov, S., Van Driel, M., Voit, R., Hekkelman, M., Hulsen, T., Hernandez, N., Grummt, I., Wehrens, R., Stunnenberg, H. (February 2007) Identification of novel functional TBP-binding sites and general factor repertoires. Embo Journal, 26 (4). pp. 944-954. ISSN 0261-4189 Emran, F., Florens, L., Ma, B., Swanson, S. K., Washburn, M. P., Hernandez, N. (August 2006) A role for Yin Yang-1 (YY1) in the assembly of snRNA transcription complexes. Gene, 377. pp. 96-108. ISSN 0378-1119 (Print) Kim, Y. S., Kim, J. M., Jung, D. L., Kang, J. E., Lee, S., Kim, J. S., Seol, W., Shin, H. C., Kwon, H. S., Van Lint, C., Hernandez, N., Hur, M. W. (June 2005) Artificial zinc finger fusions targeting Sp1-binding sites and the trans-activator-responsive element ...
Author Summary The ability of sexually reproducing organisms to produce viable offspring depends on their ability to faithfully execute meiosis. Meiosis is a specialized set of two cell divisions that ensures that each sperm and egg receives only one copy of each pair of chromosomes. Thus, in human females, although virtually all somatic cells carry 23 pairs of homologous chromosomes (for a total of 46 chromosomes), the egg needs to possess only one copy of each chromosome (for a total of 23). This reduction in chromosome number requires three basic steps: the pairing of homologous chromosomes, the linking of those pairs by recombination, and the separation of those pairs into two daughter cells at the first meiotic division. Unfortunately, little is known about the mechanism(s) by which the sites of recombination are chosen. Here we describe a Drosophila protein called Trem that both binds to meiotic chromosomes and defines the first known step of recombination initiation in Drosophila. Our studies of
hypothetical protein, PAC1, Anapl_07643, AS27_06939, AS28_03976, bcd1, B-cell-derived protein 1, C86813, CB1_000294041, cba1, copeb, core promoter element binding protein, core promoter element-binding protein, cpbp, FM2, FM6, GBF, GC-rich binding factor, GC-rich sites-binding factor GBF, Ierepo1, Ierepo3, immediate early response, erythropoietin 1, klf6-a, klf6-b, Krueppel-like factor 6, Krueppel-like factor 6-like, Krueppel-like factor 7, Kruppel-like factor 6, Kruppel-like zinc finger protein Zf9, M91_01956, M959_03695, MDA_GLEAN10016415, N300_13616, N301_13483, N302_03995, N303_03824, N305_14493, N306_00708, N307_00582, N308_06795, N309_01017, N312_07173, N320_02403, N321_14125, N322_09693, N324_04458, N325_02508, N326_01608, N327_05534, N328_08868, N329_08139, N330_10147, N331_09768, N332_08191, N334_00881, N335_03384, N336_10967, N339_05665, N340_06919, N341_10990, PAL_GLEAN10001872, PANDA_010138, proto-oncogene BCD1, protooncogene B-cell derived 1, R75280, ST12, suppression of ...
Its been a while since weve seen something from Amon Tobins alter ego - Two Fingers, but thats gonna get fixed really soon. A brand new EP. Check out Six Rhythms by Two Fingers, Noisia on Beatport. Two Fingers, the alias of our longtime friend Amon Tobin emerges on Division. Six new tracks including a Noisia feature, pressed on 1 x grams 12 vinyl. Mixmag - Premiere: Two Fingers - Cashew Rhythm (Original) Amon Tobin - Two Fingers - Saint Rhythm [Nest HQ Premiere] Amon Tobin - Two Fingers - Tasm Fet. Two Fingers, the alias of our longtime friend Amon Tobin emerges on Division. Six new tracks including a Noisia feature. Artwork by. View credits, reviews, tracks and shop for the Vinyl release of Six Rhythms on Discogs. Discover releases, reviews, credits, songs, and more about Two Fingers - Six Rhythms at Discogs. Complete your Two Fingers collection. Six Rhythms. By Two Fingers. • 6 songs. Play on Spotify. 1. Cashew Listen to Six Rhythms in full in the Spotify app. Play on Spotify. Playing. ...
ZC3H10 - ZC3H10 (GFP-tagged) - Human zinc finger CCCH-type containing 10 (ZC3H10) available for purchase from OriGene - Your Gene Company.
Notably, under conditions in which no killing of cells occurred, exposure of yeast over hundreds of generations to increasing concentrations of AmB has yielded resistant strains with permanent changes in the expression of genes such as yor1 and pdr16 (41), which are members of the ATP-binding cassette (ABC) family of transporters (9). The activation of yor1 and pdr16 is controlled by the zinc finger transcription factors Pdr1 and Pdr3, which activate proteins involved in multidrug resistance and in the translocation of plasma membrane phospholipids (9). Among the stably overexpressed genes that also confer resistance to AmB (41) are ict1, which encodes a lysophosphatidic acid acyltransferase that is responsible for enhanced phospholipid synthesis and increased resistance to antifungal drugs, and ygr035C and ypl088, which are activated by Yrm1q and Yrr1, the yeast zinc finger transcription factors which are also controlled by the pleiotropic drug resistance (PDR) gene network (9).. Another yeast ...
Buy our Recombinant Human MBD2 Interacting Zinc Finger MIZF protein. Ab161859 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA…
The binding of Au(iii) complexes to the zinc finger domain of the anticancer drug target PARP-1 was studied using a hyphenated mass spectrometry approach combined with quantum mechanics/molecular mechanics (QM/MM) studies. Competition experiments were carried out, whereby each Au complex was exposed to two t
LIM (Lin-1, Isl1, Mec3) domain proteins all contain least one double zinc finger motif (Fig. 1). LIM family proteins contain between one and five LIM domains plus other that have specific functions such as actin-binding, kinases, and nuclear translocation motifs. We are the first to examine 33 LIM proteins (including three that bind to but do not themselves contain LIM domains) that are implicated in either the development of the heart, heart disorders and failure or both.. We then assembled a cellular snapshot of the LIM proteins known to be expressed in the heart that helps explain how mutations in these proteins may play a role in the development of heart failure. Furthermore, we name the complexity of LIM domain protein interactions as a LIM interactome.. ...
Numbness in right index finger - Hi, I have a random, tingling, and numbness to my right index finger at random times of the day. I havent hit my arm or jammed my finger or any sorts? More information. Many possibilities, more information is needed. If one taps on your median nerve in the wrist does your finger tingle. If one puts pressure on the proper digital nerve in the palm or finger can you reproduce the symptoms. If the problem was in a nerve root (C6) you would also get shooting pains to from the neck down to the finger. Time will tell. It will either or worse.
Recent development in gene targeting tools makes production of knockout (KO) rabbits possible. In the present work, we generated five...
The zinc-finger transcription factor Insulinoma-associated 1 (Insm1, previously IA-1) is expressed in the developing anxious and neuroendocrine systems, and is required for cell type specific differentiation. maintained in accordance with established protocols for zebrafish husbandry (Westerfield, 1995). Larvae and Embryos had been housed at 28C, on the 14 h light:10 h dark routine. Fish had been anaesthetized with Ethyl 3-aminobenzoate methanesulfonate sodium (MS-222, Tricaine, Sigma-Aldrich, St. Louis, MO). Embryos had been staged as previously referred to (Kimmel et al., 1995). Crazy type strains included the Ekwill stress (Ekwill Fish Plantation, Gibsonton, FL), the Stomach strain extracted from the Zebrafish International Analysis Middle (ZIRC, Eugene, OR) and hybrids made by crossing the Ekwill and Stomach strains. The Tg MK-0812 (XRho: distance43-mCFP) q13 transgenic range, called XOPS-mCFP hereafter, continues to be previously referred to (Morris et al., 2011; Morris et al., 2008a). This ...
Arsenic, an ancient drug used in traditional Chinese medicine, has attracted wide interest because it has therapeutic activity in patients with acute promyelocytic leukemia (APL). The drug acts by promoting degradation of an oncogenic protein, PML-RARα, a fusion protein containing sequences from the PML zinc finger protein and retinoic acid receptor α, which is found specifically in APL cells and helps drive their growth. Zhang et al. (see the Perspective by Kogan) now explain how arsenic initiates the molecular events leading to PML-RARα degradation. Arsenic was found to bind directly to cysteine residues within zinc finger domains of PML. Arsenic binding then induced oligomerization of PML, which in turn enhanced its association with an enzyme that helps catalyze SUMOylation, a posttranslational modification that can target proteins for degradation.. X.-W. Zhang, X.-J. Yan, Z.-R. Zhou, F.-F. Yang, Z.-Y. Wu, H.-B. Sun, W.-X. Liang, A.-X. Song, V. Lallemand-Breitenbach, M. Jeanne, Q.-Y. ...
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [(PUBMED:10529348), (PUBMED:15963892), (PUBMED:15718139), (PUBMED:17210253), (PUBMED:12665246)]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both ...
Zinc finger (Znf) domains are relatively small protein motifs which contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not; instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [(PUBMED:10529348), (PUBMED:15963892), (PUBMED:15718139), (PUBMED:17210253), (PUBMED:12665246)]. Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both ...
Zinc finger protein selection[edit]. To select zinc finger proteins (ZFPs) for protein engineering, methods adapted from the ... "Highly specific zinc finger proteins obtained by directed domain shuffling and cell-based selection". Proceedings of the ... "Engineering zinc finger protein transcription factors: the therapeutic relevance of switching endogenous gene expression on or ...
Zinc-fingers[edit]. Main article: Zinc finger nuclease. Zinc-finger nucleases consist of DNA binding domains that can precisely ... Each zinc finger can recognize codons of a desired DNA sequence, and therefore can be modularly assembled to bind to a ... "Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases". Proceedings of the National Academy of ... as opposed to the time consuming assembly of constructs required by zinc-fingers or TALENs.[7] The coupled Cas9 will cause a ...
... uses a Zinc finger domain comprising five tandem Cys2His2 zinc finger motifs (meaning the zinc atom is coordinated by two ... The first finger does make numerous protein-protein interactions with the second zinc finger, however. Glis1 has an activation ... Pavletich NP, Pabo CO (September 1993). "Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers ... fourth and fifth zinc fingers are inserted into the major groove and make the most extensive contact of all the zinc fingers ...
Zinc finger UBDs have a broader range of binding modes including interactions with polar residues. Because many UBDs have a ... zinc fingers; pleckstrin homology (PH) domains; and domains similar to those in ubiquitin-conjugating (also known as E2) ...
2) Zinc finger. DNA-binding domains. 2.1. *(Intracellular receptor): Thyroid hormone resistance ...
2) Zinc finger. DNA-binding domains. 2.1. *(Intracellular receptor): Thyroid hormone resistance ...
2) Zinc finger. DNA-binding domains. 2.1. *(Intracellular receptor): Thyroid hormone resistance ...
2) Zinc finger. DNA-binding domains. 2.1. *(Intracellular receptor): Thyroid hormone resistance ...
2) Zinc finger. DNA-binding domains. 2.1. *(Intracellular receptor): Thyroid hormone resistance ... finger anomaly (e.g. short 5th finger), cleft palate, dental issues, precocious puberty, scoliosis, hip dysplasia. ...
Zinc. 6995320000000000000♠32×10−6. 0.0023. 0.00031. Yes[18][19] (e.g. Zinc finger proteins). 12 ...
2) Zinc finger. DNA-binding domains. 2.1. *(Intracellular receptor): Thyroid hormone resistance ...
2) Zinc finger. DNA-binding domains. 2.1. *(Intracellular receptor): Thyroid hormone resistance ...
2) Zinc finger. DNA-binding domains. 2.1. *(Intracellular receptor): Thyroid hormone resistance ...
ZFY (zinc finger protein). Y-chromosome-linked diseases[edit]. Diseases linked to the Y chromosome typically involve an ...
"The Zinc Finger Consortium , Consortium Members". Retrieved 2016-11-19. Maeder, Morgan L.; Thibodeau-Beganny, ... He was the leader and founder of the Zinc Finger Consortium and co-authored a study on Oligomerized Pool Engineering (OPEN), a ... In the mid-2000s, his research was focused on creating zinc finger nuclease tools for biological research and gene therapy. ... Engineering of Customized Zinc-Finger Nucleases for Highly Efficient Gene Modification". Molecular Cell. 31 (2): 294-301. doi: ...
ZNF23: encoding protein Zinc finger protein 23. *ZNF200: encoding protein Zinc finger protein 200 ... UNKL: encoding protein RING finger protein unkempt-like. *VAT1L: encoding protein Vesicle amine transport protein 1 homolog (T ... ZNF263: encoding protein Zinc finger protein 263. *ZNF629: encoding protein Zinc finger protein 629 ...
2) Zinc finger DNA-binding domains. (2.1) Nuclear receptor (Cys4). subfamily 1. *Thyroid hormone *α ...
ZBED2: encoding protein Zinc finger BED-type containing 2. *ZNF9: zinc finger protein 9 (a cellular retroviral nucleic acid ... ZMYND10: zinc finger MYND-type containing 10. *ZNF502: encoding protein Zinc finger protein 502 ...
2) Zinc finger DNA-binding domains. (2.1) Nuclear receptor (Cys4). subfamily 1. *Thyroid hormone *α ... zinc ion binding. • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific ...
2) Zinc finger DNA-binding domains. (2.1) Nuclear receptor (Cys4). .mw-parser-output .nobold{font-weight:normal}. subfamily 1. ...
Yan W, Burns KH, Ma L, Matzuk MM (Oct 2002). "Identification of Zfp393, a germ cell-specific gene encoding a novel zinc finger ...
2) Zinc finger DNA-binding domains. (2.1) Nuclear receptor (Cys4). subfamily 1. *Thyroid hormone *α ...
... is a 266 amino-acid protein and contains three C2H2 zinc finger domains.[12] OSR1 and OSR2 share 65% amino-acid sequence ... Protein odd-skipped related 1 is a zinc-finger transcription factor that, in humans, is encoded by the OSR1 gene found on ... Green RB, Hatini V, Johansen KA, Liu XJ, Lengyel JA (2002). "Drumstick is a zinc finger protein that antagonizes Lines to ... zinc finger domain similarity.[13] Function[edit]. Early expression[edit]. In mice, during gastrulation on embryological day ...
Chen X, Chu M, Giedroc DP (Sep 1999). "MRE-Binding transcription factor-1: weak zinc-binding finger domains 5 and 6 modulate ... zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal ... "A zinc-responsive factor interacts with a metal-regulated enhancer element (MRE) of the mouse metallothionein-I gene". The ...
ZNF207: encoding protein Zinc finger protein 207. *Several CC chemokines: CCL1, CCL2, CCL3, CCL4, CCL5, CCL7, CCL8, CCL11, ...
2) Zinc finger DNA-binding domains. (2.1) Nuclear receptor (Cys4). .mw-parser-output .nobold{font-weight:normal}. subfamily 1. ... "The herpes simplex virus ICP0 RING finger domain inhibits IRF3- and IRF7-mediated activation of interferon-stimulated genes" ...
Zinc finger protein 19 is a protein that in humans is encoded by the ZNF19 gene.[3][4][5] ... Thiesen HJ (1991). "Multiple genes encoding zinc finger domains are expressed in human T cells". New Biol. 2 (4): 363-74. PMID ... This gene is located in a region next to ZNF23, a gene also encoding a zinc finger protein, on chromosome 16.[5] ... The protein encoded by this gene contains a zinc finger, a nucleic acid-binding domain present in many transcription factors. ...
... zinc finger protein is a protein that in humans is encoded by the ZFP92 gene. GRCh38: Ensembl release 89: ENSG00000189420 ... "Entrez Gene: ZFP92 zinc finger protein". Mallon AM, Platzer M, Bate R, Gloeckner G, Botcherby MR, Nordsiek G, Strivens MA, ...
Zinc finger protein 300 is a protein that in humans is encoded by the ZNF300 gene. The protein encoded by this gene is a C2H2- ... "Entrez Gene: zinc finger protein 300". [email protected], Danielle Thierry-Mieg and Jean Thierry-Mieg, NCBI/NLM/NIH. " ... type zinc finger DNA binding protein and a likely transcription factor. It is antisense to the human gene, C16orf71, indicating ... "Identification and functional analysis of a novel human KRAB/C2H2 zinc finger gene ZNF300". Biochim. Biophys. Acta. 1676 (2): ...
"Entrez Gene: CASZ1 castor zinc finger 1". Human CASZ1 genome location and CASZ1 gene details page in the UCSC Genome Browser. ...
2) Zinc finger DNA-binding domains. (2.1) Nuclear receptor (Cys4). .mw-parser-output .nobold{font-weight:normal}. subfamily 1. ...
Zinc finger protein transcription factor. Failed agents. *Aplaviroc. *Atevirdine. *Brecanavir. *Capravirine. *Dexelvucitabine ...
Brandt S (May 2013). "The clinical effects of zinc as a topical or oral agent on the clinical response and pathophysiologic ... Congenital onychodysplasia of the index fingers. *Green nails. *Half and half nails ... Zinc's capacities to reduce inflammation and sebum production as well as inhibit C. acnes growth are its proposed mechanisms ... Topical and oral preparations of zinc are suggested treatments for acne; evidence to support their use for this purpose is ...
Runx2 (which may also be known as Cbfa1), and Osx (a zinc finger containing transcription factor) are necessary for ...
... zinc oxide, bismuth oxide, antimony oxide, iron rust, iron acetate, Daws (suatu contituent steel), cinnabar (HgS), arsenic ... Finger, Stanley (1994), Origins of Neuroscience: A History of Explorations Into Brain Function, Oxford University Press, m/s. ...
... pasta was eaten dry with the fingers; the liquid sauce demanded the use of a fork.[21] ...
This will induce the change of the resonant frequency of the comb finger structure. This sensor use electrostatic transduction ...
"A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre- ...
The core region has two zinc fingers that are responsible for recognizing the DNA sequences specific to this receptor. The N ...
... hydrochloric acid and zinc chloride for soldering galvanized iron (and other zinc surfaces); and borax for brazing, braze- ... adhere well to fingers. A mass of hot sticky flux can transfer more heat to skin and cause more serious burns than a comparable ... Another possibility is an inorganic flux composed of zinc chloride or tin(II) chloride,[16] ammonium chloride, and a fluoride ( ... A solution of zinc chloride in hydrochloric acid is a common flux for stainless steels; it has however to be thoroughly removed ...
sarcodactylus, a fingered citron.. *Citron varieties with sour pulp - Diamante citron, Florentine citron, Greek citron and ... zinc, or other nutrients it needs to produce chlorophyll. This condition can be cured by adding an appropriate acidic ...
zinc finger inhibitor - zinc fingers Retrieved from " ...
OSR1 · SP(1、2、4、7) · WT1 · Zbtb7(7A、7B) · ZBTB(16、17、20、32、33、40) · zinc finger(3、7、9、10、19、22、24、33B、. 34、35、41、43、44、51、74、 ... zinc ion binding. · DNA bending activity. · protein homodimerization activity. · sequence-specific DNA binding. · metal ion ...
... interacting zinc finger protein, ciz1. „Biochem. Biophys. Res. Commun.". 264 (2), s. 457-64, 1999. DOI: 10.1006/bbrc.1999.1516 ...
"The DNA ligase III zinc finger stimulates binding to DNA secondary structure and promotes end joining". Nucleic Acids Res. 28 ( ... Igual que o dedo de zinc da PARP1, o dedo de zinc da ADN ligase III está implicado na unión a roturas de febras do ADN.[6][7][8 ... é ter un dedo de zinc N-terminal que lembra os dous dedos de zinc N-terminais da poli (ADP-ribosa) polimerase 1 (PARP1).[6] ... "DNA ligase III is recruited to DNA strand breaks by a zinc finger motif homologous to that of poly(ADP-ribose) polymerase. ...
Zinc finger E-box binding homeobox 1). These are compatible with regulation relevant to lymphocytes and deregulation in cancer ...
ZFY (zinc finger protein). Y-chromosome-linked diseasesEdit. Diseases linked to the Y chromosome typically involve an ...
Finger, Andreas; Kuhr, Susanne; Engelhardt, Ulrich (1992). "Chromatography of tea constituents". Journal of Chromatography. 624 ...
... and cast metals such as pewter and zinc (spelter). But a vast number of other materials have been used as part of sculptures, ... where around 12-17,000 years ago a masterful sculptor used a spatula-like stone tool and fingers to model a pair of large bison ...
On 19 March 2015, scientists urged a worldwide ban on clinical use of methods, particularly the use of CRISPR and zinc finger, ... short fingers and toes), and hemophilia. Mathematical approaches were also devised and applied to human genetics. Population ...
Zinc 4% Copper 4% Pop culture[edit]. *Tori Amos was featured in a 1980s commercial promoting the brand. ...
2007). "An improved zinc-finger nuclease architecture for highly specific genome editing". Nature Biotechnology 25 (7): 778-785 ... 2010). "Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures". Nature Methods 8 (1): 74. ... Porteus MH, Carroll D (August 2005). "Gene targeting using zinc finger nucleases". Nat. Biotechnol. 23 (8): 967-73. PMID ... zinc finger fusions to Fok I cleavage domain". Proc Natl Acad Sci USA 93 (3): 1156-60. PMC 40048. PMID 8577732. doi:10.1073/ ...
In the United States, the Marcellus shale runs across the Southern Tier and Finger Lakes regions of New York, in northern and ... but deposited a hard mass of impure zinc oxide known as cadmia, which built up over time near the top of the flue, and had to ... the Marcellus ore occasionally contained zinc, which produced a characteristic green flame in the furnace as it was consumed, ...
Zinc anodes also need to be placed on the ship's hull. Until the mid-1900s, steel sheets were riveted together. ... The painting is usually done with lead paint (Pb3O4). Optionally, the covering with the zinc layer may be left out, but it is ... If steel is used, a zinc layer is often applied to coat the entire hull. It is applied after sandblasting (which is required to ...
... interacting zinc finger protein, ciz1". Biochem. Biophys. Res. Commun. 264 (2): 457-64. doi:10.1006/bbrc.1999.1516. PMID ...
... zinc finger protein 703); rs4849887 and rs17625845 flanking INHBB (inhibin βB); rs12173570 near ESR1 (ERα); rs7089814 in ZNF365 ... zinc finger protein 365); rs12371778 near PTHLH (parathyroid hormone-like hormone); rs62314947 near AREG (amphiregulin);[82] as ...
... synthetic proteins and synthetic zinc fingers. Using synthetic DNA, instead of there being 43 codons, if there are n new bases ...
The most common applications for engineered zinc finger arrays include zinc finger transcription factors and zinc finger ... Zinc Finger Design Tool Zinc Finger Consortium Materials from Addgene Predicting DNA-binding Specificities for C2H2 Zinc Finger ... The most common "fold groups" of zinc fingers are the Cys2His2-like (the "classic zinc finger"), treble clef, and zinc ribbon. ... Another method uses 2-finger modules to generate zinc finger arrays with up to six individual zinc fingers. The Barbas ...
It ejects the zinc from the zinc binding sites in a two steps. The zinc in the carboxyl-terminal zinc finger is released first ... Zinc finger inhibitors, or zinc ejectors, are substances or compounds that interact adversely with zinc fingers and cause them ... This method of using zinc finger inhibitors to target and destabilize zinc fingers represents a new method of fighting HIV. ... residues on the zinc finger of the NCp7 and cause a covalent conformation change which ejects the zinc from the zinc finger ...
Ascending Dose Study of Genome Editing by the Zinc Finger Nuclease (ZFN) Therapeutic SB-913 in Subjects With MPS II. The safety ...
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... Alignments can be refined by adding alignments from other genomes, adding your own ... beta-beta-alpha zinc fingers alignments. These alignments are sequences aligned to the 0052279 model. ... Home > SCOP hierarchy > beta-beta-alpha zinc fingers > Alignments for region 414-461 from F1MXA9 ...
... Alignments can be refined by adding alignments from other genomes, adding your own ... beta-beta-alpha zinc fingers alignments. These alignments are sequences aligned to the 0053891 model. ... Home > SCOP hierarchy > beta-beta-alpha zinc fingers > Alignments for region 862-914 from ENSP00000413073 ...
... Alignments can be refined by adding alignments from other genomes, adding your own ... beta-beta-alpha zinc fingers alignments. These alignments are sequences aligned to the 0050784 model. ... Home > SCOP hierarchy > beta-beta-alpha zinc fingers > Alignments for region 92-148 from G3MYC4 ...
Anti Finger Print Steel, Anti-Finger Steel Coil manufacturer supplier in , offering G550 Az150g Afp Aluminium Zinc-Alloy Hot ... G550 Az150 Anti-Finger Galvalume Coil - Galvalume Strip ...
Hot Dipped Galvalume Steel, ... Anti-Finger Aluminium Zinc Az150 Gl Galvalume Steel Coil …. China Anti-Finger Aluminium Zinc Az150 Gl Galvalume Steel Coil ... Az150 Zinc Aluminium Galvalume Anti-Finger Cold Rolled .... Az150 Zinc Aluminium Galvalume Anti-Finger Cold Rolled Steel Coil ...
anti Zinc Finger, MYM Type 2 ZMYM2. anti Zinc Finger, MYM Type 2 ZMYM2 :. Mouse Zinc finger MYM- type protein 2, Zmym2 ELISA ... Mouse RING finger and CCCH- type zinc finger domain- containing. from Lifescience Market ... Human RING finger and CCCH- type zinc finger domain- containing. from Lifescience Market ... Zinc Finger, Matrin-Type 3 Protein. from Abbexa 20-abx263236 , [ 0.1 mg: 1609.00 EUR ] [ 10 ug: 328.00 EUR ] [ 2 µg: 230.00 EUR ...
Yamaji, N., Huang, C. F., Nagao, S., Yano, M., Sato, Y., Nagamura, Y., & Ma, J. F. (2009). A zinc finger transcription factor ... A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. Plant Cell. 2009 Oct ... A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. In: Plant Cell. 2009 ... A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. / Yamaji, Naoki; ...
Mouse Zinc finger MYM-type protein 2 (ZMYM2) ELISA Kit *. Detection Target: Zinc finger MYM-type protein 2 ...
Zinc finger, CCCH-type. Mapman id. Description. 27.3.99. RNA.regulation of transcription.unclassified. ...
Compound: Zinc-finger protein ush. Species: Drosophila melanogaster [TaxId:7227]. Gene: U-shaped. Database cross-references and ... Keywords: zinc finger, GATA-1, FOG, protein-protein complex, DNA BINDING PROTEIN. Deposited on 2004-11-15, released 2005-01-25 ... Description: Zinc fingers as protein recognition motifs: structural basis for the GATA-1/Friend of GATA interaction. Class: DNA ...
ZFP57 zinc finger protein. GO Process (2). GO Function (0). GO Component (0) ...
Nuclear receptors with C4 zinc fingers. MA0112.2. MA0112. 2. ESR1. Homo sapiens Steroid hormone receptors (NR3). Nuclear ... receptors with C4 zinc fingers. MA0112.3. MA0112. 3. ESR1. Homo sapiens Steroid hormone receptors (NR3). Nuclear receptors with ...
putative zinc finger and SCAN domain-containing protein 5D. *Putative zinc finger and SCAN domain-containing protein 5D ...
Global $8.7 Bn Genome Editing Markets to 2026: Focus on CRISPR, TALENs, Zinc Finger Nucleases, Cell Line Engineering, Animal ... 23, 2021 (GLOBE NEWSWIRE) -- The Global Genome Editing Market, By Technique (CRISPR, TALENs, Zinc Finger Nucleases, Others), ...
B-box type zinc finger protein 13. GO Process (1). GO Function (2) ...
Navega Therapeutics is harnessing the precision of CRISPR and zinc finger epigenome regulation… ...
zinc finger protein 81. GO Process (1). GO Function (1). GO Component (0) ...
Product description:- product type: finger ring color: golden main material: zinc alloy gender: men & women brand: other ... Product description:- product type: finger ring color: golden main material: zinc alloy gender: women brand: other ... Gold plated dolphin ring product type: finger ring brand: local material: gold plated color: golden gender: women. ... Product description:- product type: finger ring material: gold plated good quality product gender: men and women ...
zinc finger and BTB domain containing 41. UPS Project GO Process (0) ...
For example, enzymes called transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases (ZFNs) can cut ...
The first test was developed by scientists in China and analyzed a fragment of the zinc finger protein gene. The second test, ... also using a shorter fragment of the same zinc finger protein gene, was developed by Smithsonian Conservation Biology Institute ...
Lisso, J.; Altmann, T.; Muessig, C.: The AtNFXL1 gene encodes a NF-X1 type zinc finger protein required for growth under salt ...
Fragment: Zinc Fingers (Residues 333-421). Synonym: Early Growth Response Protein 1. Engineered: Yes. Mutation: Yes. Organism_ ... Rearrangement of side-chains in a Zif268 mutant highlights the complexities of zinc finger-DNA recognition., Miller JC, Pabo CO ...
An application on a published phylogeny of KRAB zinc finger genes is presented. ... An application on a published phylogeny of KRAB zinc finger genes is presented. ...
Search zinc finger nuclease target and off-target sites ... such as for pairs of zinc finger proteins (ZFPs), zinc finger ... This can either define a zinc finger nucleases (hetero-dimer) or allow for homo-dimers depending on the status of the checkbox ...
... such as zinc finger nucleases, transcription activator-like effector nucleases, and other more traditional methods," explains ...
Through an IV, he received many copies of a corrective gene and an editing tool called zinc finger nucleases to insert it into ...
  • The HIV-1 nucleocapsid protein 7 (NCp7) is the protein targeted by zinc ejectors. (
  • In the same manner as ADA, the compounds interact with an 18-residue polypeptide on the N terminal zinc knuckle region of the HIV nucleocapsid protein which causes ejection of the zinc from the region by covalently modifying the cysteine residues. (
  • Zinc finger transcription factors or ZF-TFs , are transcription factors composed of a zinc finger - binding domain and any of a variety of transcription-factor effector-domains that exert their modulatory effect in the vicinity of any sequence to which the protein domain binds. (
  • Zinc finger protein transcription factors can be encoded by genes small enough to fit a number of such genes into a single vector , allowing the medical intervention and control of expression of multiple genes and the initiation of an elaborate cascade of events. (
  • Recognition of distinct RNA motifs by the clustered CCCH zinc fingers of neuronal protein Unkempt. (
  • The discovery of the zinc finger revealed a new protein fold but also an important aspect of DNA recognition. (
  • One application of them in 1994 used three finger protein to knock off the expression of oncogene transformed and modified into a mouse cell line. (
  • A grad student named Miller, studied TFIIIA and found out an amazing repeating motif within the protein, which was later called a zinc finger because it had zinc and attached onto the DNA. (
  • This histidine is from a peptide or protein containing a zinc finger domain. (
  • Zinc fingers are naturally occurring protein segments that recognize and bind to specific DNA sequences, typically regulating the output of a given gene. (
  • In a study published in the April 30 online version of Proceedings of the National Academy of Sciences (USA), Jae-Young Koh's group at the University of Ulsan College of Medicine in Seoul, Korea, along with collaborators in the United States, crossbred mice carrying the Swedish mutant AβPP gene with mice lacking the gene for a protein that transports zinc into synaptic vesicles. (
  • Two-finger modules are stitched together to form a Zinc Finger Protein, each with specificity of ≥ 24 bp. (
  • One approach uses zinc finger nucleases, which are artificial proteins made by joining a protein that can specifically bind DNA with an enzyme that can cleave DNA. (
  • In molecular biology the MIZ-type zinc finger domain is a zinc finger-containing protein with homology to the yeast protein, Nfi-1. (
  • In molecular biology the MYND-type zinc finger domain is a conserved protein domain. (
  • ETO has been shown to be a co-repressor recruited by the promyelocytic leukemia zinc finger (PLZF) protein. (
  • Your search returned 1 Zinc Finger Protein 500 ELISA ELISA Kit across 1 supplier. (
  • Kumagai T, Abe K, Yoshida W, Ikebukuro K (2015) DNA Detection Technology Using Zinc Finger Protein. (
  • Zinc finger protein is the major DNA binding protein in nature and it recognizes dsDNA with sequence specific manner. (
  • Using zinc finger protein for DNA detection element, simple, accurate and sensitive DNA detection can be achieved. (
  • In this review, dsDNA detection using zinc finger protein is described and compared with recent advanced technology. (
  • Recently, two groups identified a gene encoding zinc finger protein that was suggested to function as a master regulator of the neuronal phenotype. (
  • We offer Zinc finger protein 668 Peptides and Zinc finger protein 668 Proteins for use in common research applications: ELISA, Protein Array, Western Blot. (
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  • Our Zinc finger protein 668 Peptides and Zinc finger protein 668 Proteins can be used in a variety of model species: Human. (
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  • We offer Zinc finger protein 639 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. (
  • Our Zinc finger protein 639 Antibodies can be used in a variety of model species: Human. (
  • Choose from our Zinc finger protein 639 polyclonal antibodies. (
  • C2H2 zinc fingers are thought to have DNA binding and protein-protein mediation roles. (
  • Taking one step closer to understanding how, researchers led by Brian Black at the University of California, San Francisco, have uncovered a zinc finger protein, Zfp106, which associates with the fateful sequence, as well as with several RNA binding proteins implicated in the disease, including TDP-43 and FUS. (
  • As described in eLife on January 10, mice lacking Zfp106 succumb to a progressive, ALS-like disorder, while expressing the zinc finger protein in a C9ORF72 fly model suppressed motor neuron disease. (
  • Polyclonal rabbit antibodies specific for the WT1 protein were raised to an Escherichia coli-produced 91 amino acid N-terminal segment and to a 136 amino acid C-terminal segment, which contains the zinc finger domain. (
  • Zusätzlich bieten wir Ihnen Zinc Finger Protein 175 Proteine (4) und viele weitere Produktgruppen zu diesem Protein an. (
  • OTK18/zinc finger protein 175 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis. (
  • 1990). Zinc finger protein X linked is one of the members of ZFY family comprised of three members: ZFX, ZFY and ZFA (Palmer et al. (
  • To obtain an adequate amount of zinc finger proteins for studying their properties, structure, and functions, many protein expression systems are used. (
  • ZNF191(243-368) is a zinc finger protein and can be fused with His-tag to generate fusion proteins such as His 6 -ZNF191(243-368) and ZNF191(243-368)-His 8 . (
  • Our findings have furthered our understanding of the structure and folding processes of the zinc finger protein. (
  • As a proof of concept, a sequence-enabled reassembly of a TEM-1 β-lactamase system (SEER-LAC) was previously demonstrated to develop zinc finger protein (ZFP) arrays for the detection of a double-stranded bacterial DNA sequence. (
  • A complementary DNA was isolated that encodes a zinc finger-containing protein that suppressed IL-2 gene expression. (
  • The Zinc-finger protein ASCIZ regulates B cell development via DYNLL1 and Bim. (
  • The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. (
  • As the most abundant DNA binding protein in humans, thousands of naturally occurring zinc finger proteins exist to recognize and bind specific DNA sequences, with each finger binding three DNA nucleotides or "bases" at a time. (
  • Zinc finger protein transcription factors (ZFP-TFs) are used to regulate gene expression by either activating or repressing the activity of a gene or an individual gene allele. (
  • ZFP-TFs are engineered by attaching an activation or repression domain to a designed zinc finger protein. (
  • Rabbit Anti-Mouse Zinc Finger Protein of Cerebellum 2 Polyclonal Antibody, Unconjugated from Meridian Life Science, Inc. (
  • A gene on chromosome 13q22 that encodes a member of the Kruppel-like zinc finger protein family that can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. (
  • BRISBANE, Calif.--( BUSINESS WIRE )-- Sangamo Therapeutics Inc. (Nasdaq: SGMO), a genomic medicine company, today announced the publication of a manuscript describing the activity of allele-selective zinc finger protein transcription-factors (ZFP-TFs) in preclinical models of Huntington's disease (HD). (
  • PATZ1 is a zinc finger protein, belonging to the POZ domain Krüppel-like zinc finger (POK) family of architectural transcription factors, first discovered in 2000 by three independent groups. (
  • Here we explore a novel approach to neurotrophic factor-based therapy by engineering zinc finger protein transcription factors (ZFP TFs) that activate the expression of the endogenous glial cell line-derived neurotrophic factor (GDNF) gene. (
  • The rat zinc-finger antiviral protein (ZAP) was recently identified as a host protein conferring resistance to retroviral infection. (
  • A previously unknown rat protein, designated zinc-finger antiviral protein (ZAP), was recently found to exhibit antiviral activity against Moloney murine leukemia virus (MMLV), a member of the Retroviridae . (
  • Here we have identified a highly conserved Zinc finger DNA binding protein CNBP (also called ZNF9) upregulated in myeloid cells exposed to lipopolysaccharide. (
  • One gene isolated was opl, (odd-paired-like) that resembles the Drosophila pair-rule gene odd-paired and encodes a zinc finger protein that is a member of the Zic gene family. (
  • 1994 ) A novel zinc finger protein, zic, is involved in neurogenesis, especially in the cell lineage of cerebellar granule cells. (
  • a zinc finger pair-rule protein required for the timely activation of engrailed and wingless in Drosophila embryos. (
  • OBJECTIVE The objectives of the study were to evaluate retrograde axonal transport of vascular endothelial growth factor A (VEGF-A) protein to sensory neurons after intramuscular administration of an engineered zinc finger protein activator of endogenous VEGF-A (VZ+434) in an experimental model of diabetes, and to characterize the VEGF-A target neurons. (
  • Given the importance of all VEGF-A isoforms, we have chosen to investigate an alternative strategy for the therapeutic application of this growth factor, namely, the activation of the endogenous VEGF-A gene through the action of an engineered zinc finger protein-transcription factor (ZFP-TF). (
  • METHODOLOGY/PRINCIPAL FINDINGS: Using the genome-scale in vivo DNA methylation data from human brain, we investigated the flanking sequence features of methylation-resistant CpG islands, and discovered that there are several over-represented putative Transcription Factor Binding Sites (TFBSs) in methylation-resistant CpG islands, and a specific group of zinc finger protein binding sites are over-represented in boundary regions ( approximately 400 bp) flanking such CpG islands. (
  • CONCLUSIONS/SIGNIFICANCE: Our results suggest a possible mechanism that certain putative zinc finger protein binding sites over-represented in the boundary regions of the methylation-resistant CpG islands may block the spreading of methylation into these islands, and those TFBSs over-represented within the islands may both reinforce the methylation blocking and promote transcription. (
  • Here we report that the promyelocytic leukemia zinc finger (PLZF) protein can interact with GATA-2 and can modify its transactivation capacity. (
  • Fanconi anemia zinc finger (FAZF), a PLZF-homologous protein that has been variously described as ROG (repressor of GATA), and TZFP (testis zinc finger protein) also interact with GATA-2. (
  • Although the t(15;17) translocation accounts for most APL cases, variant translocations have been described including the t(11;17) translocation, which fuses the promyelocytic zinc finger protein (PLZF) to RARα. (
  • One of the first breakthrough methods of gene targeting was the usage of chimeric proteins called zinc-fingers nucleases (ZFN) to create double-strand breaks. (
  • Zinc-finger proteins (ZNFs) are one of the most abundant groups of proteins and have a wide range of molecular functions. (
  • Given the wide variety of zinc-finger domains, ZNFs are able to interact with DNA, RNA, PAR (poly-ADP-ribose) and other proteins. (
  • Zinc-finger proteins (ZNFs) are involved in several cellular processes acting through different molecular mechanisms. (
  • In classical C2H2 zinc-finger proteins, two cysteines in one chain and two histidines in other one are coordinated by a zinc ion. (
  • The most important and abundant types of zinc-finger domain proteins include C2H2, really interesting new gene (RING), plant homeodomain (PHD), and Lin-ll, Isl-1, and Mec-3 (LIM domains). (
  • In contrast to other DNA binding proteins that usually utilize the two-fold symmetry o the double helix, zinc finger are bonded together in a single file to recognize nucleic acid sequences of differing lengths. (
  • The zinc finger's most suitable role is for engineering proteins to find specific genes and target them. (
  • In addition, it is possible that putting zinc fingers with the other effector domains like from nucleases or integrases, to make chimeric proteins, genomes could be controlled and modified. (
  • There are a couple applications of engineered zinc finger proteins, which can have therapeutic importance. (
  • Zinc finger nucleases (ZFNs) are proteins engineered to make site-specific double-strand breaks (DSBs) in a DNA sequence of interest. (
  • NEW ORLEANS - Sangamo BioSciences , the Richmond, California company that has sped ahead with engineered 'zinc fingers', is pointing to these proteins as a powerful tools in treating an ever-increasing list of illnesses. (
  • Using genetic engineering, the Sangamo researchers designed zinc finger proteins containing a DNA-binding site that recognizes the prolonged tricnucleotide repeat found in the mutant Htt gene. (
  • This is the first attempt to apply the zinc finger approach to Huntington's disease, and the researchers eventually aim to deliver genes for the zinc finger proteins directly into the brain using adeno-associated viral vectors*, which are already being used to successfully deliver therapeutic genes into the brains of people with Parkinson's disease in clinical trials . (
  • In a paper published this week in Science, Isabelle Barde of the EPFL's School of Life Sciences and Frontiers in Genetic Program, and colleagues, describe experiments which show that KRAB-containing zinc finger proteins , working in concert with a cofactor called KAP1, modulate mitophagy in subtle and sophisticated ways. (
  • 2000 Differential effects of toxic metal compounds on the activities of Fpg and XPA, two zinc finger proteins involved in DNA repair. (
  • Richmond, CA) has been granted a new patent in the United States entitled "Selection of Sites for Targeting by Zinc Finger Proteins and Methods of Designing Zinc Finger Proteins to Bind to Preselected Sites. (
  • Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. (
  • Consortium members Keith Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing zinc finger proteins for distribution to academic and nonprofit laboratories for research and educational use. (
  • Cartoon representation of the zinc-finger motif of proteins. (
  • Zinc finger nucleases (ZFNs) are proteins composed of a DNA binding-domain and a DNA cleaving-domain. (
  • Several alternatively spliced REST/NRSF/XBR mRNAs encoding proteins with nine, five, or four zinc finger motifs are transcribed from REST/NRSF/XBR gene. (
  • Zinc finger nucleases (ZFNs) are synthetic proteins commonly used in genome engineering that contain an custom-designed zinc finger DNA-binding domain fused to the cleavage domain of the FokI endonuclease. (
  • Custom zinc finger proteins can be designed to target a specific DNA sequence in a gene of interest in nearly any cell type. (
  • The 5' of CASZ1 contains 2 nuclear localization signals, which tag proteins for import into the cell nucleus, followed by 5 zinc fingers of C2H2 type (Cys 2 His 2 ). (
  • Zinc finger proteins (ZNFs) bind DNA and, through this binding, regulate gene transcription. (
  • Krüppel-related zinc finger proteins constitute the largest single class of transcription factors within the human genome. (
  • Approximately one third of the Krüppel-related zinc finger proteins contain an evolutionarily well-conserved repressor domain termed the KRAB domain. (
  • In order to identify novel KRAB-containing zinc finger proteins, one mouse monocytic cDNA library and two testis cDNA libraries were screened for novel members of this multigene family. (
  • The corresponding proteins were all shown to contain N-terminally located KRAB domains as well as varying numbers of C-terminally located zinc finger motifs. (
  • Zinc finger proteins are associated with hereditary diseases and cancers. (
  • Zinc finger proteins have been expressed, separated, and purified using the His-tag/Ni-NTA system [ 4 - 6 ]. (
  • These drugs induce rapid ubiquitination and proteasomal degradation of two transcription factors, Ikaros (IKZF1) and Aiolos (IKZF3), by recruiting them to the CRL4 CRBN E3 ubiquitin ligase through a Cys2-His2 (C2H2) zinc finger (ZF) domain that is present in both proteins and required for their destruction. (
  • DNA-binding specificity of modular zinc-finger proteins (ZFPs). (
  • Zinc finger domains, which recognize double-stranded DNA, can be engineered to form custom DNA-binding proteins for the recognition of specific DNA sequences. (
  • The Arabidopsis LSD1 and LOL1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (PCD). (
  • Evolutionary success of natural zinc finger proteins, however, evinces that nature created specific evolutionary traits and strategies, such as modularity and rank-specific recognition to cope with binding complexity that are critical for creating clinical viable tools to precisely modify the human genome. (
  • Naturally occurring zinc finger proteins enable our cells to pinpoint a gene among thousands of others and to regulate its expression. (
  • By attaching different functional domains to those location-specific arrays of zinc fingers, or zinc finger proteins, Sangamo is able to edit and repair DNA, or adjust the expression level of a particular gene to treat serious diseases. (
  • ZFNs are engineered by attaching a nuclease, a DNA cutting enzyme, to a pair of zinc finger proteins. (
  • The human genome encodes about 700 proteins with Cys2-His2 zinc finger (C2H2-ZF) domains, the majority of which are likely to bind to DNA and regulate transcription. (
  • Zinc-finger containing proteins, however, do not appear to follow this general rule and have instead undergone very rapid diversification. (
  • The human genome encodes some 350 Kruppel-associated box (KRAB) domain-containing zinc-finger proteins (KZFPs), the products of a rapidly evolving gene family that has been traced back to early tetrapods(1,2). (
  • The zinc finger region of GATA-2 is required for binding to PLZF and FAZF, but distinct interfaces on the PLZF and FAZF molecules mediate the interaction, suggesting that GATA-2 activity is controlled by these 2 homologous proteins through distinct mechanisms. (
  • GATA factors comprise a family of transcriptional regulatory proteins characterized by the ability to bind a common conserved DNA sequence (W GATA R) by virtue of evolutionarily conserved C4 zinc finger domains. (
  • The NCps (nucleocapsid proteins) of HIV-1 (HIV type 1), HIV-2 and SIV (simian immunodeficiency virus) are small highly basic proteins, characterized by the presence of two CCHC ZF (zinc finger) domains. (
  • Recently, targeted cleavage of the genome using engineered DNA scissors called zinc finger nucleases (ZFNs) has successfully supported the precise manipulation of genetic information in various cells, animals, and plants. (
  • Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. (
  • Townsend JA et al (2009) High-frequency modification of plant genes using engineered zinc-finger nucleases. (
  • Zhang F et al (2010) High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. (
  • Shukla VK et al (2009) Precise genome modification in the crop species Zea mays using zinc-finger nucleases. (
  • Osakabe K, Osakabe Y, Toki S (2010) Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases. (
  • Doyon Y et al (2008) Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. (
  • Maeder ML et al (2008) Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification. (
  • Two new techniques identify how often zinc fingers nucleases cleave off-target sites. (
  • Zinc finger nucleases are designed to be like heat-seeking missiles, precisely targeted to find and cut specific sequences of DNA. (
  • As we begin to treat patients with zinc finger nucleases and modify genomes, we need to know where those modifications are being made. (
  • A superficial interpretation of our paper might lead one to be pessimistic about zinc finger nucleases, but actually I'm optimistic," Liu said. (
  • al, "Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection," Nature Methods, doi:10.1038/nmeth.1670, 2011. (
  • In the study published in Science titled "Knockout Rats via Embryo Microinjection of Zinc Finger Nucleases," (Geurts, et al. (
  • The procedure that generates these high frequencies relies on cleavage of the target by designed zinc-finger nucleases (ZFNs) and production of a linear donor in situ . (
  • We report very high gene targeting frequencies in Drosophila by direct embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). (
  • Zinc-finger nucleases (ZFNs) are proving to be powerful tools for directed genome manipulation ( 4 , 5 ). (
  • Graphic shows that with the increasing amount of zinc finger nucleases + integrase-defective lentiviral vectors (ZFN-IDLVs) used for desired gene delivery in human stem cells, the frequency of stem cells carrying the genes, GFP and Puro increases. (
  • Compared to older gene-replacement methods, it was discovered that gene-correction methods like those involving zinc finger nucleases and integrase-defective lentiviral vectors (ZFN-IDLVs) were safer and more effective for gene therapy. (
  • Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. (
  • Zinc finger nucleases (ZFNs), which consist of an engineered zinc finger DNA-binding domain fused to the DNA cleavage domain of the FokI enzyme, are powerful tools for performing genome engineering in a variety of organisms. (
  • Such a technique includes Zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered, regularly-interspaced, short palindromic repeats/CRISPR-associated endonuclease cas9 (CRISPR/Cas9). (
  • In the present work, we generated five Apolipoprotein (Apo) C-III KO rabbits using Zinc Finger Nucleases (ZFN). (
  • Zinc finger nucleases (ZFNs) are associated with cell death and apoptosis by binding at countless undesired locations. (
  • Zinc finger nucleases (ZFNs) enable genome editing, which may be designed to delete a certain gene or sequence of DNA and/or add a new, therapeutic gene or sequence of DNA at a precise location. (
  • Kim S et al (2011) Preassembled zinc-finger arrays for rapid construction of ZFNs. (
  • A complete list of ZNF types with a description of the zinc-finger domain structure, the number of genes included, and the most studied members is summarized in Table 1 . (
  • Therefore, genes can be switched on or off in a carefully chosen manner through the fusion of zinc finger peptides to repression or activation domains. (
  • By stringing together several zinc fingers and adding a DNA-cleaving nuclease, researchers can precisely target specific genes to be cut. (
  • Because zinc fingers can be directed to a broad range of DNA sequences and targeting is very efficient, this approach promises to allow genetic manipulation of many different genes, even in cases where the mutant phenotype cannot be predicted. (
  • The identification of a common cis -acting silencer element, a neuron-restrictive silencer element (NRSE), in multiple neuron-specific genes, together with the finding that zinc finger transcription factor REST/NRSF/XBR could confer NRSE-mediated silencing in non-neuronal cells, suggested that REST/NRSF/XBR is a master negative regulator of neurogenesis. (
  • One of the novel human cDNAs, HKr19, was found to be a member of the large ZNF91 family of KRAB zinc finger genes. (
  • The predicted sequence of the IAP polypeptide exhibited no significant homology to P35 but contained a zinc finger-like motif which is also found in other genes with the potential to regulate apoptosis, including several mammalian proto-oncogenes and two insect genes involved in embryonic development. (
  • a ) A schematic representation of the structure of C2H2, RING, PHD, and LIM zinc-finger domains. (
  • Contains 9 C2H2-type zinc fingers. (
  • contains 8 C2H2-type zinc fingers. (
  • LZ: leucine-rich domain SR: serine rich domain PR: Proline rich domain NLS: Nuclear Localization signal ZFs: Kruppel-type C2H2 zinc finger domains QA: Gln-Ala repeat (See also Martini et al. (
  • ZFs, Kruppel-type C2H2 zinc finger domains QA: Gln-Ala repeat (see also Martini et al. (
  • Most ZNFs contain conserved C2H2 motifs and are classified as Kruppel-type zinc fingers. (
  • The small molecules thalidomide, lenalidomide, and pomalidomide induce the ubiquitination and proteasomal degradation of the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3) by recruiting a Cys-His (C2H2) zinc finger domain to Cereblon (CRBN), the substrate receptor of the CRL4 E3 ubiquitin ligase. (
  • We screened the human C2H2 zinc finger proteome for degradation in the presence of thalidomide analogs, identifying 11 zinc finger degrons. (
  • Structural and functional characterization of the C2H2 zinc finger degrons demonstrates how diverse zinc finger domains bind the permissive drug-CRBN interface. (
  • C2H2-ZF domains are short sequences that contain a beta-hairpin and an alpha-helix stabilized by a zinc ion, with only two cysteines and two histidines that are invariable. (
  • Other viruses such as SARS, polio, Ebola, measles, human coxsackie, Dengue, rabies, human hepatitis, human parainfluenza and human respiratory syncytical have similar zinc finger motifs and could potentially benefit from zinc finger inhibitor technology. (
  • These motifs contain two peptide units of Cys-X2-Cys-X4-His-X4-Cys (CCHC), where the X represents a substituted amino acid, that make up the zinc (II) ion binding sites. (
  • Yang M, May WS, Ito T: JAZ requires the double-stranded RNA-binding zinc finger motifs for nuclear localization. (
  • It contains four zinc finger motifs that encode putative DNA binding domains. (
  • Zinc fingers are the most common family of transcription factors in organisms ranging from yeast to humans. (
  • Computer model showing the complex of the zinc finger transcription factors GATA-1 (orange) and FOG-1 (friend of GATA, blue). (
  • Previous work showed that the drugs bind to the E3 ubiquitin ligase Cereblon, which then targets for degradation two specific zinc finger (ZF) transcription factors with a role in cancer development. (
  • 2001 ). Together with its paralog ZEB1, ZEB2 belongs to the Zeb family of transcription factors characterized by two zinc finger clusters separated by a homeodomain. (
  • ZNF24, also known as ZNF191 ( 14 , 15 ) and KOX17 ( 16 ), is a relatively uncharacterized transcription factor belonging to the family of SCAN box domain-containing Krüppel-like Cys 2 -His 2 zinc finger transcription factors ( 14 , 16 ). (
  • Exon 10 contains 3' UTR and AATAAA polyadenylation signal and encodes C-terminal zinc - finger domain for isoforms 1 and 2 (Schneider-Gadicke et al. (
  • For example, there is no straightforward and easy way to construct zinc finger domains to bind a comprehensive stretch of nucleotides with high affinity. (
  • Zinc fingers, so named because their structure resembles a hand with a pointed finger, bind to different three-letter nucleotide sequences. (
  • Computational zinc finger docking and biochemical analysis predict that more than 150 zinc fingers bind the drug-CRBN complex in vitro, and we show that selective zinc finger degradation can be achieved through compound modifications. (
  • Selection of zinc fingers that bind single-stranded telomeric DNA in the G-quadruplex conformation," Biochemistry, vol. 40, No. 3, Jan. 23, 2001, pp. 830-836. (
  • This cytotoxicity is associated with the binding ability of engineered zinc finger domains to bind dissimilar DNA sequences with high affinity. (
  • A new study uncovers a potential mechanism that may allow zinc-finger domains in metazoans to recognize and bind virtually any DNA sequence. (
  • 5 ] observed that not only could different zinc-finger sequences bind to the same DNA triplet, but the same zinc-finger could also recognize several different DNA targets. (
  • 1. An isolated Cys2 His2 zinc finger polypeptide that binds to a molecule selected from the group consisting of telomeric nucleic acid, G-quadruplex nucleic acid and G-quartetnucleic acid. (
  • The binding of zinc (II) in the CCHC binding site is necessary for the domain to be functional and for the stabilization of the conformation of the structure, allowing the NCp7 to carry out the processes required for HIV replication. (
  • They react with the cysteine residues on the zinc finger of the NCp7 and cause a covalent conformation change which ejects the zinc from the zinc finger domain. (
  • The binding specificity of the designed zinc-finger domain points the ZFN to a specific genomic site. (
  • Currently, 30 types of ZNFs are approved by The HUGO Gene Nomenclature Committee, 9 and ZNF classification is based on the zinc-finger domain structure. (
  • A zinc finger is a self-contained domain that formed through the stabilization of a zinc ion binded to a pair of cysteines and a pair of histidines. (
  • Kim YG, Cha J, Chandrasegaran S (1996) Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. (
  • Here, we report a biophysical and structural characterization of the F1 and F2 fingers of human PARP-1, both as independent fragments and in the context of the 24-kDa DNA-binding domain (F1+F2). (
  • A DNA-binding domain comprised of a chain of two-finger modules, each recognizing a unique hexamer (6 bp) sequence of DNA. (
  • The zinc fingers are part of the binding-domain and have precisely identified and bound to a group of matching nucleobases in the DNA sequence. (
  • One zinc "finger" binding domain recognizes and binds to a three nucleotide sequence, one can thus increase the binding specificity of a ZFN by adding more zinc fingers. (
  • The MYND domain consists of a cluster of cysteine and histidine residues, arranged with an invariant spacing to form a potential zinc-binding motif. (
  • The results indicated that HKr19, or its zinc finger domain in isolation, were toxic to these cells when expressed at high levels. (
  • Acidic domain (orange), nuclear localisation signal (blue) and zinc finger domain (green) are labelled. (
  • 2012). Isoforms I and II contain three domains: acidic domain (transcription activation region), nuclear localization signal (NLS) and 13 zinc finger domains, that differ in the length of their N-terminal (acidic) domain resulting in qualitatively and quantitatively different regulatory properties (Schneider-Gadicke et al. (
  • ZF, zinc-finger domain. (
  • Our findings indicate preservation of general modularity and significant alteration of the rank-specific binding preferences of the three-finger binding domain of transcription factor SP1 when exchanging amino acids in the 2nd finger. (
  • The binding of Au( III ) complexes to the zinc finger domain of the anticancer drug target PARP-1 was studied using a hyphenated mass spectrometry approach combined with quantum mechanics/molecular mechanics (QM/MM) studies. (
  • Notably, the cyclometallated Au-C^N complex was identified as the most selective candidate to disrupt the PARP-1 zinc finger domain, forming distinct adducts compared to the coordination compound Auphen. (
  • By analyzing experimental data for thousands of zinc-finger domain sequences, they discovered that it is the contribution of residues that do not make direct contact with the bases that does the trick. (
  • In other words, given the sequence of a zinc-finger domain, can we predict its DNA target? (
  • PLZF has a pox virus and zinc finger (POZ) domain and 9 homologous C2H4 zinc fingers that mediate binding to the GTACT-AGTAC motif in DNA. (
  • Since monkeys are of great interest for clinical studies of antiviral drugs, the structure of (13-51)NCp8 (zinc finger domain of NCp8, encompassing residues 13-51) from SIVlhoest was determined by NMR to appraise the influence of major differences in the sequence, since Glu21, Gly43 and Met46 in NCp7 are replaced by Pro, Glu and Phe respectively in this particular NCp8. (
  • Until this time there hasn't been a really comprehensive way of defining zinc finger nuclease specificity," said Carlos Barbas , a chemical biologist at the Scripps Research Institute in La Jolla, Calif., who was not involved in the study. (
  • Such specificity raises the possibility of developing zinc finger nuclease (ZFN) gene therapies. (
  • al, "An unbiased genome-wide analysis of zinc-finger nuclease specificity," Nature Biotechnology , doi:10.1038/nbt.1948, 2011. (
  • Sangamo uses a library of more than 3,200 zinc finger modules to engineer potential therapeutic tools for targeting a specific location in the human genome. (
  • Deletion analysis of Ins2 promoter identifies a sequence (5'-GTCCCCTGCTGTGAA-3') from -255 to -241 as the Glis3 response element and binding occur specifically via the Glis3 zinc finger region as revealed by mobility shift assays. (
  • B ) Sequence logos for ZFP monomers recognizing GAG in each finger position. (
  • C ) Sequence logos for ZFPs containing TTG modules at the indicated finger position. (
  • Peptides were examined by CD spectropolarimetry (0.25 mM peptide in 25 mM phosphate buffer, pH 7.0) in the presence and absence of zinc. (
  • Ran binds with the highest affinity to the second zinc finger motif of Nup153 (Nup153ZnF2). (
  • When TFIIIA associates with DNA, it binds along the major groove with each finger binding about five nucleotides of DNA. (
  • Using a combination of NMR spectroscopy and other biophysical techniques, we show that recognition is primarily achieved by F2, which binds the DNA in an essentially identical manner whether present in isolation or in the two-finger fragment. (
  • The paper supports earlier work by Bush, Huang, Atwood and Cherny showing that zinc binds avidly to Aβ and induces its precipitation in vitro, and that chelation therapy reverses amyloid deposition in AβPP-trangenic mice (see ARF news story). (
  • Zinc finger inhibitors, or zinc ejectors, are substances or compounds that interact adversely with zinc fingers and cause them to release their zinc from its binding site, disrupting the conformation of the polypeptide chain and rendering the zinc fingers ineffective, thereby preventing them from performing their associated cellular functions. (
  • NCp7 is initially formed as part of the gag polypeptide and follows a gag-knuckle zinc finger conformation. (
  • 2. A zinc finger polypeptide according to claim 1 wherein said nucleic acid is not in a double-helical conformation. (
  • 3. A zinc finger polypeptide according to claim 1 wherein said nucleic acid comprises single-stranded DNA. (
  • 4. A zinc finger polypeptide according to claim 1 wherein said nucleic acid is contained in a chromosome end. (
  • 5. A zinc finger polypeptide according to claim 1 wherein said nucleic acid is in a non-Watson-Crick base paired conformation. (
  • 6. A zinc finger polypeptide according to claim 1 wherein said nucleic acid comprises Hoogsteen base pairing. (
  • 7. A zinc finger polypeptide according to claim 1 wherein said zinc finger polypeptide comprises at least one zinc finger motif. (
  • 8. A zinc finger polypeptide according to claim 1 wherein said polypeptide has an affinity for G-quadruplex nucleic acid which is different from its affinity for duplex nucleic acid. (
  • and (iv) monitoring the binding of said zinc finger polypeptide to said telomerase treated nucleic acid sample. (
  • 15. A method for discriminating between duplex and quadruplex nucleic acid comprising contacting a sample of nucleic acid with a zinc finger polypeptide according to claim 8 and monitoring the binding of said zinc finger polypeptide to saidnucleic acid. (
  • We have expressed a 92-amino acid polypeptide containing the three N-terminal zinc fingers of TFIIIA. (
  • This three-fingered polypeptide has been isotopically labeled with N-15 and C-13 in E. coli and purified to homogeneity. (
  • all of the materials in this accessory reagents kit are included in the Zinc Finger Consortium Modular Assembly Kit (# 1000000005) and therefore do not need to be purchased in addition to the Modular Assembly Kit. (
  • The broad-complex tramtrack and bric a brac-zinc finger transcriptional regulator(BTB-ZF), promyelocytic leukemia zinc finger (PLZF), was recently shown to control the development of the characteristic innate T cell phenotype and effector functions of NK T cells. (
  • Zinc Finger Nuclease (ZFN) technology has provided researchers with a tool for integrating exogenous sequences into most cell lines or genomes in a precise manner. (
  • Thus, in principle, zinc-finger domains can be designed to target a broad range of DNA sequences. (
  • They are named for how they use a zinc atom to fold into a compact shape that can fit inside the major groove of DNA, enabling recognition of specific base sequences. (
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  • Kit #1000000005 includes 141 zinc finger plasmids encoding different zinc finger modules from the Sangamo, Barbas, and Toolgen module sets, and can be used to efficiently assemble zinc finger arrays via modular assembly. (
  • The zinc ion (green) is coordinated by two histidine and two cysteine amino acid residues. (
  • Najafabadi and colleagues [ 3 ] used information on the residues directly contacting the DNA bases to expand the zinc-finger-DNA recognition code. (
  • Crystallographic studies revealed that classical zinc-finger domains have two β -sheets and one α-helix. (
  • b ) A schematic representation of the structure of some ZNFs with multiple zinc-finger domains. (
  • Each Zinc Finger Nuclease (ZFN) consists of two functional domains: a. (
  • In May 2008, the laboratories of Keith Joung, Daniel Voytas, and Toni Cathomen published a large-scale evaluation of the modular assembly method and reported important guidance for researchers interested in using this approach to engineer multi-finger domains. (
  • To attempt to decipher the code, two studies [ 3 , 5 ] used the one-hybrid system to estimate the DNA-binding affinities of thousands of natural zinc-finger domains. (
  • To overcome this, Sangamo researchers have developed zinc finger transcriptional repressors that specifically target the mutant Htt allele and block its expression while preserving near-normal expression levels of the normal allele. (
  • In the current study, we show that the zinc finger transcription factor ZNF24 may represent a novel transcriptional repressor of VEGF expression. (
  • Zinc finger nuclease mediated gene targeting of a single copy gp91 phox therapeutic minigene into one allele of the "safe harbor" AAVS1 locus in X-CGD iPSCs without off-target inserts resulted in sustained expression of gp91 phox and substantially restored neutrophil ROS production. (
  • Also included in this kit are 5 plasmids and a strain for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. (
  • The Richmond company (NASDAQ: SGMO) will receive $20 million upfront to use its gene-editing zinc finger nuclease platform to fix the abnormal structure or underproduction of hemoglobin. (
  • We show that the fingers are structurally independent in the absence of DNA and share a highly similar structural fold and dynamics. (
  • Structural and functional studies show that either deletion of the N-terminus or mutation in any of the first four zinc fingers of CASZ1b results in a drastic loss in transcriptional activity, which also leads to a decreased tumor suppression activity (Virden et al. (
  • The results show that each finger folds into a canonical beta-sheet-helix zinc finger structural motif, while the linkers adopt an extended structure. (
  • Our analysis is focused on comparison with experimental and quantum mechanical data, concerning the local Zn-finger and overall structural and dynamic properties for these models. (
  • ADA inhibits HIV by electophilically attacking the sulfur atoms of the zinc coordinated cysteine. (
  • Non-classical types of zinc-finger differ in cysteine/histidine combinations, such as C2-H2, C2-CH, and C2-C2. (
  • Eighteen amino acid peptide adopts zinc finger conformation in the presence of zinc. (
  • In the absence of zinc as shown in a , histidine peaks appear at 7.08 and 7.91, which shift upon addition of zinc to those characteristic of metal bound histidine at 7.01 and 7.58 ( b ). (
  • The helix between the two histidine ligands in ZF3 is distorted by zinc coordination, to accommodate the presence of four intervening amino acids instead of three as in ZF1 and ZF2. (
  • Scientists from The Medical College of Wisconsin in Milwaukee, Sangamo Biosciences, Inc., (NASDAQ: SGMO), Sigma-Aldrich Corporation (NASDAQ: SIAL), Open Monoclonal Technology, Inc. (OMT) and INSERM today announced the creation of the first genetically modified mammals developed using zinc finger nuclease (ZFN) technology. (
  • Here, we identify a zinc finger transcription factor, zDC, which is evolutionarily conserved and specifically expressed by cDC but not monocytes or other immune populations. (
  • CASZ1 is a crucial zinc finger transcription factor that controls the differentiation of neural (Cui and Doe, 1992) and cardiac muscle cells (Christine and Conlon, 2008), eye development (Vacalla and Theil, 2002) and has tumor suppressor properties (Liu et al. (
  • DNA of CASZ1a contains 160072 bp composed of 21 exons with 11 zinc fingers, alternative splicing at exon 16 of CASZ1a can result in CASZ1b (16 exons with 5 zinc fingers) (Liu et al. (
  • 2004). "Attenuation of HIV-1 replication in primary human cells with a designed zinc finger transcription factor" . (
  • For some time, in vitro and human research has indicated that heavy metals such as zinc, copper, or iron, are critical for the aggregation of Aβ into plaques. (
  • Ramirez CL et al (2008) Unexpected failure rates for modular assembly of engineered zinc fingers. (
  • In July 2012, a legacy version of the ZiFiT software package (ZiFiT v3.3) was made available to maintain support for modular assembly of zinc fingers using the Modular Assembly Kit developed by the Zinc Finger Consortium (Wright et al. (