AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesAnthropology, Education, Sociology and Social PhenomenaInformation Science
ZebrafishZebrafish ProteinsEmbryo, NonmammalianAnimals, Genetically ModifiedGene Expression Regulation, DevelopmentalMorpholinosIn Situ HybridizationBody PatterningLarvaAnimal FinsLateral Line SystemMolecular Sequence DataRhombencephalonSomitesEmbryonic DevelopmentAmino Acid SequenceGene Knockdown TechniquesMutationMorphogenesisGreen Fluorescent ProteinsOligonucleotides, AntisenseNodal Signaling LigandsHomeodomain ProteinsGastrulationNotochordPhylogenySequence Homology, Amino AcidPhenotypeSignal TransductionBase SequenceTranscription FactorsBranchial RegionCloning, MolecularOrganogenesisBlastulaModels, AnimalMesodermSequence AlignmentMelanophoresHedgehog ProteinsTakifuguDiencephalonRNA, MessengerPronephrosNeural CrestRegenerationRetinaOryziasTailSOX Transcription FactorsSwimmingCell DifferentiationMicroinjectionsNervous SystemDNA, ComplementaryAir SacsOligodeoxyribonucleotides, Antisensep-AminoazobenzenePAX2 Transcription FactorConserved SequenceDisease Models, AnimalT-Box Domain ProteinsReceptors, NotchEndodermVertebratesEyeFibroblast Growth FactorsBone Morphogenetic ProteinsFibroblast Growth Factor 3Fish ProteinsEar, InnerTetraodontiformesReverse Transcriptase Polymerase Chain ReactionVeratrum AlkaloidsBasic Helix-Loop-Helix Transcription FactorsGrowth Differentiation Factor 6Central Nervous SystemCell MovementJaw AbnormalitiesCell LineageNerve Tissue ProteinsGenomeWnt ProteinsDNA PrimersEmbryonic InductionTransgenesHeartRadiation Hybrid MappingGene Expression ProfilingBlood VesselsGillsGene ExpressionTrans-ActivatorsCiliaBehavior, AnimalModels, Biological
Zebrafish
An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
Zebrafish Proteins
Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).
Embryo, Nonmammalian
The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.
Animals, Genetically Modified
ANIMALS whose GENOME has been altered by GENETIC ENGINEERING, or their offspring.
Gene Expression Regulation, Developmental
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Morpholinos
Synthetic analogs of NUCLEIC ACIDS composed of morpholine ring derivatives (MORPHOLINES) linked by phosphorodimidates. One standard DNA nucleic acid base (ADENINE; GUANINE; CYTOSINE; OR THYMINE) is bound to each morpholine ring.
In Situ Hybridization
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
Body Patterning
The processes occurring in early development that direct morphogenesis. They specify the body plan ensuring that cells will proceed to differentiate, grow, and diversify in size and shape at the correct relative positions. Included are axial patterning, segmentation, compartment specification, limb position, organ boundary patterning, blood vessel patterning, etc.
Larva
Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.
Animal Fins
Membranous appendage of fish and other aquatic organisms used for locomotion or balance.
Lateral Line System
Aquatic vertebrate sensory system in fish and amphibians. It is composed of sense organs (canal organs and pit organs) containing neuromasts (MECHANORECEPTORS) that detect water displacement caused by moving objects.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Rhombencephalon
The posterior of the three primitive cerebral vesicles of an embryonic brain. It consists of myelencephalon, metencephalon, and isthmus rhombencephali from which develop the major BRAIN STEM components, such as MEDULLA OBLONGATA from the myelencephalon, CEREBELLUM and PONS from the metencephalon, with the expanded cavity forming the FOURTH VENTRICLE.
Somites
Paired, segmented masses of MESENCHYME located on either side of the developing spinal cord (neural tube). Somites derive from PARAXIAL MESODERM and continue to increase in number during ORGANOGENESIS. Somites give rise to SKELETON (sclerotome); MUSCLES (myotome); and DERMIS (dermatome).
Embryonic Development
Morphological and physiological development of EMBRYOS.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Gene Knockdown Techniques
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Mutation
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Morphogenesis
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
Green Fluorescent Proteins
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Oligonucleotides, Antisense
Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
Nodal Signaling Ligands
Members of the transforming growth factor superfamily that play a role in pattern formation and differentiation during the pregastrulation and GASTRULATION stages of chordate development. Several nodal signaling ligands are specifically involved in the genesis of left-right asymmetry during development. The protein group is named after a critical region of the vertebrate embryo PRIMITIVE STREAK referred to as HENSEN'S NODE.
Homeodomain Proteins
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
Gastrulation
A process of complicated morphogenetic cell movements that reorganizes a bilayer embryo into one with three GERM LAYERS and specific orientation (dorsal/ventral; anterior/posterior). Gastrulation describes the germ layer development of a non-mammalian BLASTULA or that of a mammalian BLASTOCYST.
Notochord
A cartilaginous rod of mesodermal cells at the dorsal midline of all CHORDATE embryos. In lower vertebrates, notochord is the backbone of support. In the higher vertebrates, notochord is a transient structure, and segments of the vertebral column will develop around it. Notochord is also a source of midline signals that pattern surrounding tissues including the NEURAL TUBE development.
Phylogeny
The relationships of groups of organisms as reflected by their genetic makeup.
Sequence Homology, Amino Acid
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Phenotype
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Transcription Factors
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Branchial Region
A region, of SOMITE development period, that contains a number of paired arches, each with a mesodermal core lined by ectoderm and endoderm on the two sides. In lower aquatic vertebrates, branchial arches develop into GILLS. In higher vertebrates, the arches forms outpouchings and develop into structures of the head and neck. Separating the arches are the branchial clefts or grooves.
Cloning, Molecular
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Organogenesis
Formation of differentiated cells and complicated tissue organization to provide specialized functions.
Blastula
An early non-mammalian embryo that follows the MORULA stage. A blastula resembles a hollow ball with the layer of cells surrounding a fluid-filled cavity (blastocele). The layer of cells is called BLASTODERM.
Models, Animal
Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.
Mesoderm
The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Melanophores
Chromatophores (large pigment cells of fish, amphibia, reptiles and many invertebrates) which contain melanin. Short term color changes are brought about by an active redistribution of the melanophores pigment containing organelles (MELANOSOMES). Mammals do not have melanophores; however they have retained smaller pigment cells known as MELANOCYTES.
Hedgehog Proteins
A family of intercellular signaling proteins that play and important role in regulating the development of many TISSUES and organs. Their name derives from the observation of a hedgehog-like appearance in DROSOPHILA embryos with genetic mutations that block their action.
Takifugu
A genus of pufferfish commonly used for research.
Diencephalon
The paired caudal parts of the PROSENCEPHALON from which the THALAMUS; HYPOTHALAMUS; EPITHALAMUS; and SUBTHALAMUS are derived.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Pronephros
The primordial kidney that develops from the INTERMEDIATE MESODERM in the embryos of vertebrates, and is succeeded by the MESONEPHROS. In higher vertebrates and humans, the pronephros is a vestigial and transient structure.
Neural Crest
The two longitudinal ridges along the PRIMITIVE STREAK appearing near the end of GASTRULATION during development of nervous system (NEURULATION). The ridges are formed by folding of NEURAL PLATE. Between the ridges is a neural groove which deepens as the fold become elevated. When the folds meet at midline, the groove becomes a closed tube, the NEURAL TUBE.
Regeneration
The physiological renewal, repair, or replacement of tissue.
Retina
The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.
Oryzias
The only genus in the family Oryziinae, order BELONIFORMES. Oryzias are egg-layers; other fish of the same order are livebearers. Oryzias are used extensively in testing carcinogens.
Tail
SOX Transcription Factors
A large family of structurally-related transcription factors that were originally discovered based upon their close sequence homology to an HMG-box domain found in SEX-DETERMINING REGION Y PROTEIN. Many SOX transcription factors play important roles in regulating CELL DIFFERENTIATION. The numerous members of this family are organized in several subgroups according to structural identities found within the proteins.
Swimming
An activity in which the body is propelled through water by specific movement of the arms and/or the legs. Swimming as propulsion through water by the movement of limbs, tail, or fins of animals is often studied as a form of PHYSICAL EXERTION or endurance.
Cell Differentiation
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Microinjections
The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes.
Nervous System
The entire nerve apparatus, composed of a central part, the brain and spinal cord, and a peripheral part, the cranial and spinal nerves, autonomic ganglia, and plexuses. (Stedman, 26th ed)
DNA, Complementary
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Air Sacs
Thin-walled sacs or spaces which function as a part of the respiratory system in birds, fishes, insects, and mammals.
Oligodeoxyribonucleotides, Antisense
Short fragments of DNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
p-Aminoazobenzene
Used in the form of its salts as a dye and as an intermediate in manufacture of Acid Yellow, diazo dyes, and indulines.
PAX2 Transcription Factor
A paired box transcription factor that is essential for ORGANOGENESIS of the CENTRAL NERVOUS SYSTEM and KIDNEY.
Conserved Sequence
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
Disease Models, Animal
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
T-Box Domain Proteins
Proteins containing a region of conserved sequence, about 200 amino acids long, which encodes a particular sequence specific DNA binding domain (the T-box domain). These proteins are transcription factors that control developmental pathways. The prototype of this family is the mouse Brachyury (or T) gene product.
Receptors, Notch
A family of conserved cell surface receptors that contain EPIDERMAL GROWTH FACTOR repeats in their extracellular domain and ANKYRIN repeats in their cytoplasmic domains. The cytoplasmic domain of notch receptors is released upon ligand binding and translocates to the CELL NUCLEUS where it acts as transcription factor.
Endoderm
The inner of the three germ layers of an embryo.
Vertebrates
Animals having a vertebral column, members of the phylum Chordata, subphylum Craniata comprising mammals, birds, reptiles, amphibians, and fishes.
Eye
The organ of sight constituting a pair of globular organs made up of a three-layered roughly spherical structure specialized for receiving and responding to light.
Fibroblast Growth Factors
A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.
Bone Morphogenetic Proteins
Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Fibroblast Growth Factor 3
A fibroblast growth factor that is expressed primarily during development.
Fish Proteins
Proteins obtained from species of fish (FISHES).
Ear, Inner
The essential part of the hearing organ consists of two labyrinthine compartments: the bony labyrinthine and the membranous labyrinth. The bony labyrinth is a complex of three interconnecting cavities or spaces (COCHLEA; VESTIBULAR LABYRINTH; and SEMICIRCULAR CANALS) in the TEMPORAL BONE. Within the bony labyrinth lies the membranous labyrinth which is a complex of sacs and tubules (COCHLEAR DUCT; SACCULE AND UTRICLE; and SEMICIRCULAR DUCTS) forming a continuous space enclosed by EPITHELIUM and connective tissue. These spaces are filled with LABYRINTHINE FLUIDS of various compositions.
Tetraodontiformes
A small order of primarily marine fish containing 340 species. Most have a rotund or box-like shape. TETRODOTOXIN is found in their liver and ovaries.
Reverse Transcriptase Polymerase Chain Reaction
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Veratrum Alkaloids
Alkaloids with powerful hypotensive effects isolated from American or European Hellebore (Veratrum viride Ait. Liliaceae and Veratrum album L. Liliaceae). They increase cholinergic and decrease adrenergic tone with appropriate side effects and at higher doses depress respiration and produce cardiac arrhythmias; only the ester alkaloids have been used as hypotensive agents in specific instances. They have been generally replaced by drugs with fewer adverse effects.
Basic Helix-Loop-Helix Transcription Factors
A family of DNA-binding transcription factors that contain a basic HELIX-LOOP-HELIX MOTIF.
Growth Differentiation Factor 6
A growth differentiation factor that plays a role in the neural differentiation, specifically in the retinal development of the EYE.
Central Nervous System
The main information-processing organs of the nervous system, consisting of the brain, spinal cord, and meninges.
Cell Movement
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Jaw Abnormalities
Congenital absence of or defects in structures of the jaw.
Cell Lineage
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
Nerve Tissue Proteins
Genome
The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.
Wnt Proteins
Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.
DNA Primers
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Embryonic Induction
The complex processes of initiating CELL DIFFERENTIATION in the embryo. The precise regulation by cell interactions leads to diversity of cell types and specific pattern of organization (EMBRYOGENESIS).
Transgenes
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
Heart
The hollow, muscular organ that maintains the circulation of the blood.
Radiation Hybrid Mapping
A method for ordering genetic loci along CHROMOSOMES. The method involves fusing irradiated donor cells with host cells from another species. Following cell fusion, fragments of DNA from the irradiated cells become integrated into the chromosomes of the host cells. Molecular probing of DNA obtained from the fused cells is used to determine if two or more genetic loci are located within the same fragment of donor cell DNA.
Gene Expression Profiling
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Blood Vessels
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
Gills
Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment.
Gene Expression
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Trans-Activators
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Cilia
Populations of thin, motile processes found covering the surface of ciliates (CILIOPHORA) or the free surface of the cells making up ciliated EPITHELIUM. Each cilium arises from a basic granule in the superficial layer of CYTOPLASM. The movement of cilia propels ciliates through the liquid in which they live. The movement of cilia on a ciliated epithelium serves to propel a surface layer of mucus or fluid. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Behavior, Animal
The observable response an animal makes to any situation.
Models, Biological
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

FGF8 induces formation of an ectopic isthmic organizer and isthmocerebellar development via a repressive effect on Otx2 expression. (1/4712)

Beads containing recombinant FGF8 (FGF8-beads) were implanted in the prospective caudal diencephalon or midbrain of chick embryos at stages 9-12. This induced the neuroepithelium rostral and caudal to the FGF8-bead to form two ectopic, mirror-image midbrains. Furthermore, cells in direct contact with the bead formed an outgrowth that protruded laterally from the neural tube. Tissue within such lateral outgrowths developed proximally into isthmic nuclei and distally into a cerebellum-like structure. These morphogenetic effects were apparently due to FGF8-mediated changes in gene expression in the vicinity of the bead, including a repressive effect on Otx2 and an inductive effect on En1, Fgf8 and Wnt1 expression. The ectopic Fgf8 and Wnt1 expression domains formed nearly complete concentric rings around the FGF8-bead, with the Wnt1 ring outermost. These observations suggest that FGF8 induces the formation of a ring-like ectopic signaling center (organizer) in the lateral wall of the brain, similar to the one that normally encircles the neural tube at the isthmic constriction, which is located at the boundary between the prospective midbrain and hindbrain. This ectopic isthmic organizer apparently sends long-range patterning signals both rostrally and caudally, resulting in the development of the two ectopic midbrains. Interestingly, our data suggest that these inductive signals spread readily in a caudal direction, but are inhibited from spreading rostrally across diencephalic neuromere boundaries. These results provide insights into the mechanism by which FGF8 induces an ectopic organizer and suggest that a negative feedback loop between Fgf8 and Otx2 plays a key role in patterning the midbrain and anterior hindbrain.  (+info)

The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping. (2/4712)

Left-right asymmetry in vertebrates is controlled by activities emanating from the left lateral plate. How these signals get transmitted to the forming organs is not known. A candidate mediator in mouse, frog and zebrafish embryos is the homeobox gene Pitx2. It is asymmetrically expressed in the left lateral plate mesoderm, tubular heart and early gut tube. Localized Pitx2 expression continues when these organs undergo asymmetric looping morphogenesis. Ectopic expression of Xnr1 in the right lateral plate induces Pitx2 transcription in Xenopus. Misexpression of Pitx2 affects situs and morphology of organs. These experiments suggest a role for Pitx2 in promoting looping of the linear heart and gut.  (+info)

A Drosophila doublesex-related gene, terra, is involved in somitogenesis in vertebrates. (3/4712)

The Drosophila doublesex (dsx) gene encodes a transcription factor that mediates sex determination. We describe the characterization of a novel zebrafish zinc-finger gene, terra, which contains a DNA binding domain similar to that of the Drosophila dsx gene. However, unlike dsx, terra is transiently expressed in the presomitic mesoderm and newly formed somites. Expression of terra in presomitic mesoderm is restricted to cells that lack expression of MyoD. In vivo, terra expression is reduced by hedgehog but enhanced by BMP signals. Overexpression of terra induces rapid apoptosis both in vitro and in vivo, suggesting that a tight regulation of terra expression is required during embryogenesis. Terra has both human and mouse homologs and is specifically expressed in mouse somites. Taken together, our findings suggest that terra is a highly conserved protein that plays specific roles in early somitogenesis of vertebrates.  (+info)

Alzheimer's disease: clues from flies and worms. (4/4712)

Presenilin mutations give rise to familial Alzheimer's disease and result in elevated production of amyloid beta peptide. Recent evidence that presenilins act in developmental signalling pathways may be the key to understanding how senile plaques, neurofibrillary tangles and apoptosis are all biochemically linked.  (+info)

Isolation of zebrafish gdf7 and comparative genetic mapping of genes belonging to the growth/differentiation factor 5, 6, 7 subgroup of the TGF-beta superfamily. (5/4712)

The Growth/differentiation factor (Gdf) 5, 6, 7 genes form a closely related subgroup belonging to the TGF-beta superfamily. In zebrafish, there are three genes that belong to the Gdf5, 6, 7 subgroup that have been named radar, dynamo, and contact. The genes radar and dynamo both encode proteins most similar to mouse GDF6. The orthologous identity of these genes on the basis of amino acid similarities has not been clear. We have identified gdf7, a fourth zebrafish gene belonging to the Gdf5, 6, 7 subgroup. To assign correct orthologies and to investigate the evolutionary relationships of the human, mouse, and zebrafish Gdf5, 6, 7 subgroup, we have compared genetic map positions of the zebrafish and mammalian genes. We have mapped zebrafish gdf7 to linkage group (LG) 17, contact to LG9, GDF6 to human chromosome (Hsa) 8 and GDF7 to Hsa2p. The radar and dynamo genes have been localized previously to LG16 and LG19, respectively. A comparison of syntenies shared among human, mouse, and zebrafish genomes indicates that gdf7 is the ortholog of mammalian GDF7/Gdf7. LG16 shares syntenic relationships with mouse chromosome (Mmu) 4, including Gdf6. Portions of LG16 and LG19 appear to be duplicate chromosomes, thus suggesting that radar and dynamo are both orthologs of Gdf6. Finally, the mapping data is consistent with contact being the zebrafish ortholog of mammalian GDF5/Gdf5.  (+info)

A molecular pathway revealing a genetic basis for human cardiac and craniofacial defects. (6/4712)

Microdeletions of chromosome 22q11 are the most common genetic defects associated with cardiac and craniofacial anomalies in humans. A screen for mouse genes dependent on dHAND, a transcription factor implicated in neural crest development, identified Ufd1, which maps to human 22q11 and encodes a protein involved in degradation of ubiquitinated proteins. Mouse Ufd1 was specifically expressed in most tissues affected in patients with 22q11 deletion syndrome. The human UFD1L gene was deleted in all 182 patients studied with 22q11 deletion, and a smaller deletion of approximately 20 kilobases that removed exons 1 to 3 of UFD1L was found in one individual with features typical of 22q11 deletion syndrome. These data suggest that UFD1L haploinsufficiency contributes to the congenital heart and craniofacial defects seen in 22q11 deletion.  (+info)

Expression of CD44 in Apc and Tcf mutant mice implies regulation by the WNT pathway. (7/4712)

Overexpression of cell surface glycoproteins of the CD44 family is an early event in the colorectal adenoma-carcinoma sequence. This suggests a link with disruption of APC tumor suppressor protein-mediated regulation of beta-catenin/Tcf-4 signaling, which is crucial in initiating tumorigenesis. To explore this hypothesis, we analyzed CD44 expression in the intestinal mucosa of mice and humans with genetic defects in either APC or Tcf-4, leading to constitutive activation or blockade of the beta-catenin/Tcf-4 pathway, respectively. We show that CD44 expression in the non-neoplastic intestinal mucosa of Apc mutant mice is confined to the crypt epithelium but that CD44 is strongly overexpressed in adenomas as well as in invasive carcinomas. This overexpression includes the standard part of the CD44 (CD44s) as well as variant exons (CD44v). Interestingly, deregulated CD44 expression is already present in aberrant crypt foci with dysplasia (ACFs), the earliest detectable lesions of colorectal neoplasia. Like ACFs of Apc-mutant mice, ACFs of familial adenomatous polyposis (FAP) patients also overexpress CD44. In sharp contrast, Tcf-4 mutant mice show a complete absence of CD44 in the epithelium of the small intestine. This loss of CD44 concurs with loss of stem cell characteristics, shared with adenoma cells. Our results indicate that CD44 expression is part of a genetic program controlled by the beta-catenin/Tcf-4 signaling pathway and suggest a role for CD44 in the generation and turnover of epithelial cells.  (+info)

Comparative synteny cloning of zebrafish you-too: mutations in the Hedgehog target gli2 affect ventral forebrain patterning. (8/4712)

Zebrafish you-too (yot) mutations interfere with Hedgehog (Hh) signaling during embryogenesis. Using a comparative synteny approach, we isolated yot as a zinc finger transcription factor homologous to the Hh target gli2. Two alleles of yot contain nonsense mutations resulting in carboxy-terminally truncated proteins. In addition to causing defects in midline development, muscle differentiation, and retinal axon guidance, yot mutations disrupt anterior pituitary and ventral forebrain differentiation. yot mutations also cause ectopic lens formation in the ventral diencephalon. These findings reveal that truncated zebrafish Gli2 proteins interfere with Hh signaling necessary for differentiation and axon guidance in the ventral forebrain.  (+info)

HKU Scholars Hub: Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryosHKU Scholars Hub: Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos
We investigated the mechanisms of intermediate cell mass (ICM) expansion in zebrafish chordin (Chd) morphant embryos and examined the role of BMPs in relation to this phenotype. At 24 h post-fertilization (hpf), the expanded ICM of embryos injected with chd morpholino (MO) (Chd MO embryos) contained a monotonous population of hematopoietic progenitors. In situ hybridization showed that hematopoietic transcription factors were ubiquitously expressed in the ICM whereas vascular gene expression was confined to the periphery. BMP4 (but not BMP2b or 7) and smad5 mRNA were ectopically expressed in the Chd MO ICM. At 48 hpf, monocytic cells were evident in both the ICM and circulation of Chd MO but not WT embryos. While injection of BMP4 MO had no effect on WT hematopoiesis, co-injecting BMP4 with chd MOs significantly reduced ICM expansion. Microarray studies revealed a number of genes that were differentially expressed in Chd MO and WT embryos and their roles in hematopoiesis has yet to be ...
http://hub.hku.hk/handle/10722/76317
The role of gamma interferon in innate immunity in the zebrafish embryo | Disease Models & MechanismsThe role of gamma interferon in innate immunity in the zebrafish embryo | Disease Models & Mechanisms
The zebrafish genome contains ten genes that encode class II cytokine-like peptides, of which the two that are related most closely to mammalian interferon gamma (IFN-γ) were named IFN-γ1 and IFN-γ2. Although the zebrafish has become a popular model system to study immune mechanisms, and although interferons are central regulators of immunity, which zebrafish cytokines correspond functionally to mammalian IFN-γ has not been established. We used zebrafish embryos to assay the functions of IFN-γ1 and IFN-γ2, and have identified a subset of zebrafish homologs of the mammalian IFN-responsive genes as IFN-γ targets in the zebrafish embryo: these genes are upregulated in response to raised levels of either IFN-γ1 or IFN-γ2. Infection studies using two different pathogens show that IFN-γ signalling is required for resistance against bacterial infections in the young embryo and that the levels of IFN-γ need to be regulated tightly: raising IFN-γ levels sensitizes fish embryos against ...
http://dmm.biologists.org/content/early/2009/09/21/dmm.003509
Akap12beta supports asymmetric heart development via modulating the Kupffers vesicle formation in zebrafish
Kupffers vesicle (KV) is a spherical left-right organizer which appears transiently during an embryonic stage in zebrafish (1). KV originates from a cluster of dorsal forerunner cells (DFCs). Cadherin1 (Cdh1)-mediated adherens junctions sustain cell cluster formation between the adjacent DFCs and a cluster of DFCs actively migrates towards the vegetal pole to form KV (2). Then, migrated DFCs attach to the overlying surface epithelium and become polarized to construct a rosette-like structure which contains the lumen at the apical point (3). Finally, cilia-formed and fluid-filled KV expands the internal lumen and motile cilia generate fluid flow in a counterclockwise direction to evoke asymmetric signal(s) such as Nodal, Lefty, and Pitx2 (1).. The vertebrate body plan is accomplished by left-right asymmetric organ development. The heart is a representative asymmetric internal organ which jogs to the left-side and proper positioning during embryonic development is crucial for its function (4). ...
https://www.bmbreports.org/journal/view.html?uid=1407&vmd=Full
zebrafish model review paperszebrafish model review papers
and Characterization of zebrafish von Willebrand factor reveals conservation of domain structure, multimerization, and intracellular storage by J. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. Maintaining the Barrier Function of Blood Vessels. I. Zebrafish are a promising model for obesity research, as lipid metabolism pathways are conserved between mammals and fish [44-46].Zebrafish have the key organs that are important for energy homeostasis and metabolism in mammals, as well as other key functions such as appetite regulation in the brain [], insulin regulation [], endocrine signaling through leptin [], and … The present review provides a brief overview of recent studies on wild‐type and transgenic zebrafish as a model system to monitor toxic heavy metals, endocrine disruptors, and organic pollutants for toxicology. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, ...
http://fiorebeauty.com/khq8u8/9ab44c-zebrafish-model-review-papers
Zebrafish In Drug DiscoveryZebrafish In Drug Discovery
Interest in using zebrafish is on the rise for several reasons. Zebrafish are small and inexpensive to maintain, and they produce hundreds of offspring in every mating, easily accumulating statistically significant numbers of animals. Whats more, zebrafish embryos are transparent and develop outside the uterus, making it easy to monitor developing fish. In fact, zebrafish embryos are tiny enough to be raised in microplate wells for high-throughput whole-animal assays. Many test compounds can diffuse into zebrafish embryos, simplifying drug administration. Researchers can also readily identify zebrafish with gene mutations. Lastly, as vertebrates, zebrafish and other fish have most of the same organs and organ systems that mammals do, making them more relevant in whole-animal assays than fruit flies or roundworms, which have also been used in early drug discovery screening.. ...
https://cen.acs.org/articles/85/i39/Zebrafish-Drug-Discovery.html
zebrafish and human genome similarity
The team identified 154 pseudogenes in the zebrafish genome, a fraction of the 13,000 or so pseudogenes found in the human genome. In fact, 70% of human genes are found in zebrafish . Practically speaking they offer many advantages as research models: The understanding of several human diseases has already grown leaps and bounds because of zebrafish studies. Disruption of enhancer function has been shown to lead to abnormal gene expression and thus to disease (2-4). The genetic underpinnings of heart development in zebrafish are highly similar to that in humans, while zebrafish presents many advantages that allow for rapid screening of … This paper focuses on … Hopkins and her colleagues found that a gene called met, which is known to cause cancer, sits on a chromosome found in excess in zebrafish tumors.,Other researchers had previously observed met on a chromosome found in extra numbers in human cancer. This high degree of similarity has led to the broad use of zebrafish to study the ...
http://www.synergy.com.sa/4nwrzprr/zebrafish-and-human-genome-similarity-6b9ae1
Talk:Zebrafish Development - Embryology
J Vis Exp. 2012 Dec 16;(70). pii: 4243. doi: 10.3791/4243. Chang JT, Sive H. Source Department of Biology, Whitehead Institute of Biomedical Research, Massachusetts Institute of Technology. Abstract Cerebrospinal fluid (CSF) is a protein rich fluid contained within the brain ventricles. It is present during early vertebrate embryonic development and persists throughout life. Adult CSF is thought to cushion the brain, remove waste, and carry secreted molecules(1,2). In the adult and older embryo, the majority of CSF is made by the choroid plexus, a series of highly vascularized secretory regions located adjacent to the brain ventricles(3-5). In zebrafish, the choroid plexus is fully formed at 144 hours post fertilization (hpf)(6). Prior to this, in both zebrafish and other vertebrate embryos including mouse, a significant amount of embryonic CSF (eCSF) is present . These data and studies in chick suggest that the neuroepithelium is secretory early in development and may be the major source of ...
https://embryology.med.unsw.edu.au/embryology/index.php?title=Talk:Zebrafish_Development&oldid=251202
Different Wavelengths of Light Have No Effect on Zebrafish Fecundity - Matters
Danio rerio (zebrafish) have rapidly gained popularity in the study of vertebrate development and gene function. Zebrafish are an excellent model for performing large-scale genetic and drug screens to be performed coupled with the visualization of downstream perturbations on live, developing organisms. Due to the fact that large numbers of embryos are required to perform these experiments, we investigated ways to efficiently increase zebrafish embryo production while not being overly invasive or using chemicals that might affect the developmental processes. To perform these experiments, we mated wild type (wt) zebrafish while exposing them to the different wavelengths of light. Fish were exposed to 12 h of dark, followed by 4 h with the different colored light treatments. Once this light cycle was over, embryo production was enumerated. Results indicated that exposing zebrafish to the different wavelengths of fluorescent and LED light had little effect on embryo production. Further research may
https://sciencematters.io/articles/201904000001
Receptor-interacting serine/threonine-protein kinase 4 (ripk4) induces epidermal differentiation in early zebrafish embryos...Receptor-interacting serine/threonine-protein kinase 4 (ripk4) induces epidermal differentiation in early zebrafish embryos...
The epithelium is the outer most layer of cells responsible for mediating the interactions of an organism with its external environment. Squamous cells are scale-like flattened cells that form the most superficial layer of the epithelium. Regulatory errors in these cells can lead to squamous cell carcinoma, the second most common form of skin cancer. Understanding of the underlying molecular mechanisms is integral for improved diagnostics and therapeutics. IKK1 is a known regulator of several important pathways including regulation of epidermal differentiation and proliferation. Changes in IKK1 expression have been linked to tumor proliferation in squamous cell carcinomas. IKK1 -/- knockout mice have proven critical in understanding the function of IKK1 but are difficult in studies of early epidermal development. The novel zebrafish maternal-effect mutant poky contains a mutation in the zebrafish homolog ikk1. This study focuses on a related protein kinase called Ripk4. The mouse homolog RIP4 is ...
https://cache.kzoo.edu/handle/10920/19837
Katharine Lewis Candidate Statement - International Zebrafish Society
Associate Professor, Biology Department. Syracuse University (Syracuse, New York). Service: I have been actively involved in the zebrafish community for my whole career. Ive attended all but one of the international zebrafish meetings since my first international zebrafish conference in 1996, all of the strategic conferences for zebrafish investigators held in the US and a couple of the European PI meetings. Recently I hosted and co-organized (with Jeff Amack and Jason Myers) the first ever Central New York zebrafish conference. I have been a PhD examiner for many zebrafish theses, an ad hoc reviewer for zebrafish grants for 12 different funding agencies in the US, Europe and Hong Kong and an NSF panel member for IOS Neural Organization. I was an elected committee member for the British Society for Developmental Biology for 5 years and was a scientific organizer for the annual BSDB conference in 2010 and a Frontiers of Science Symposium co-funded by the Royal Society (UK) and Japanese Society ...
https://izfs.site-ym.com/page/LEWISSTATEMENT
K-ras/PI3K-Akt signaling is essential for zebrafish hematopoiesis and angiogenesis<...
TY - JOUR. T1 - K-ras/PI3K-Akt signaling is essential for zebrafish hematopoiesis and angiogenesis. AU - Liu, Lihui. AU - Zhu, Shizhen. AU - Gong, Zhiyuan. AU - Low, Boon Chuan. PY - 2008/8/6. Y1 - 2008/8/6. N2 - The RAS small GTPases orchestrate multiple cellular processes. Studies on knock-out mice showed the essential and sufficient role of K-RAS, but not N-RAS and H-RAS in embryonic development. However, many physiological functions of K-RAS in vivo remain unclear. Using wild-type and fli1:GFP transgenic zebrafish, we showed that K-ras-knockdown resulted in specific hematopoietic and angiogenic defects, including the impaired expression of erythroid-specific gene gatal and βe3-hemoglobin, reduced blood circulation and disorganized blood vessels. Expression of either K-rasC40 that links to phosphoinositide 3-kinase (P13K) activation, or Akt2 that acts downstream of P13K, could rescue both hematopoietic and angiogenic defects in the K-ras knockdown. Consistently, the functional rescue by ...
https://mayoclinic.pure.elsevier.com/en/publications/k-raspi3k-akt-signaling-is-essential-for-zebrafish-hematopoiesis-
Zebrafish Health and Welfare Glossary Home - Zebrafish Health and Welfare Glossary - ZFIN Community WikiZebrafish Health and Welfare Glossary Home - Zebrafish Health and Welfare Glossary - ZFIN Community Wiki
Welcome to the Zebrafish Welfare Terms Wiki. The zebrafish welfare terms WIKI provides a standardised approach to the language used across facilities when describing zebrafish characteristics, which have the potential to impact welfare. We hope that by using these standardised terms we raise awareness of potential health concerns specific to genetic background and environmental impacts, which affect the well-being of zebrafish in our facilities and help bridge communication between different constituents involved in animal care. The terms have been created, reviewed, and added to by experienced animal caretakers and veterinarians.. If you would like to add to or edit this document please email one of these contacts. Further information regarding zebrafish welfare terms can be found in: Standardised Welfare Terms for the Zebrafish Community (http://zfin.org/ZDB-PUB-160421-6).. Goodwin et al_zeb_2016_1248.pdf. ...
https://wiki.zfin.org/display/ZHWG/Zebrafish+Health+and+Welfare+Glossary+Home
Chi-Bin Chien Award - International Zebrafish Society
The Chi-Bin Chien Award was established by the zebrafish research community and the International Zebrafish Society (IZFS) in memory of Dr. Chi-Bin Chien (1965-2011). Chi-Bin was Professor of Neurobiology and Anatomy at the University of Utah and served the international zebrafish community in numerous ways, including his service as Director of the Zebrafish Neural Development and Genetics Course at the Woods Hole Marine Biological Laboratory and as an organizer of the International Conference on Zebrafish Genetics and Development. The Award celebrates Chi-Bins enthusiasm and love for the discussion of scientific ideas and the good things that grow out of interactions and open discourse among scientists, his efforts to mentor and support the development of young scientists, and the collaborative and generous spirit with which he contributed to advance research with the zebrafish. The award will be given to an outstanding graduate student, postdoctoral trainee, or recently appointed faculty ...
https://izfs.site-ym.com/page/CBCAwardNew
WIDESPREAD STRUCTURAL AND CODING SEQUENCE VARIATION HIGHLIGHT RAPID AND ONGOING EVOLUTION OF ZEBRAFISH IMMUNE GENES - NACIW 2019WIDESPREAD STRUCTURAL AND CODING SEQUENCE VARIATION HIGHLIGHT RAPID AND ONGOING EVOLUTION OF ZEBRAFISH IMMUNE GENES - NACIW 2019
2. Center for Research Informatics, The University of Chicago, 5751 S. Woodlawn Avenue, Chicago, IL 60637, USA. Zebrafish excel as a model organism for developmental biology and disease modeling. Traditionally, laboratory zebrafish have been maintained as outbred populations with high genetic variability. Our recent work has examined the core MHC locus in zebrafish, where we found alternative pathways of antigen processing and presentation genes that are separated by 500 million years of evolution. Here we performed highcoverage genomic sequencing for two clonal lines of zebrafish, and one partially inbred zebrafish, to uncover additional sources of immune gene variation throughout these genomes. Pathway analysis identified immune genes as highly enriched among genes under positive selection, or associated with structural variation. Overall, zebrafish genomes are enriched by approximately 5 fold higher levels of variation compared with humans, including SNVs, small indels, and structural ...
http://naciworkshop.com/session/widespread-structural-and-coding-sequence-variation-highlight-rapid-and-ongoing-evolution-of-zebrafish-immune-genes/
Induction of axial mesoderm by zDVR-1, the zebrafish orthologue of Xenopus Vg1Induction of axial mesoderm by zDVR-1, the zebrafish orthologue of Xenopus Vg1
The zebrafish DVR-1 (zDVR-1) gene, like Xenopus Vg1, is present maternally as an unprocessed precursor protein which is distributed ubiquitously along the future dorsoventral axis. Also, like Vg1, overexpression of zDVR-1 in zebrafish directs synthesis of more precursor, but no processed protein. Ho …
https://pubmed.ncbi.nlm.nih.gov/8608857/?dopt=Abstract
Human Stem Cell Exposure to Developmental Stage Zebrafish Extracts: a Novel Strategy for Tuning Stemness and Senescence...Human Stem Cell Exposure to Developmental Stage Zebrafish Extracts: a Novel Strategy for Tuning Stemness and Senescence...
INTRODUCTION. Zebrafish maintains a remarkably higher ability than mammals to repair complex tissues after injury, including the heart and the central nervous system. For this reason, zebrafish embryos and their stem cells have been increasingly studied to unravel the molecular mechanisms underlying such regenerative potential, or to dissect evolutionary conserved pathways that may account for the regenerative action afforded by stem cells across different species.. Transplantation of human cord blood-derived CD34+ (hCD34+) cells into pregastrulation zebrafish embryos revealed that these human cells cosegregated with presumptive zebrafish hemangioblasts, being involved in early development of the embryonic vasculature of the recipient 1. Conversely, postgastrulation transplant resulted in the recruitment of hCD34+ cells into developing vessels, where their biology was mainly shifted to a paracrine action 2. These human cells were also found to accelerate vascular repair in adult zebrafish, after ...
http://www.cellr4.org/article/1226
PLOS ONE: Reverse Genetics Screen in Zebrafish Identifies a Role of miR-142a-3p in Vascular Development and IntegrityPLOS ONE: Reverse Genetics Screen in Zebrafish Identifies a Role of miR-142a-3p in Vascular Development and Integrity
MicroRNAs are a well-studied class of non-coding RNA and are known to regulate developmental processes in eukaryotes. Their role in key biological processes such as vasculature development has attracted interest. However, a comprehensive understanding of molecular regulation of angiogenesis and vascular integrity during development remains less explored. Here we identified miRNAs involved in the development and maintenance of vasculature in zebrafish embryos using a reverse genetics approach. Using a combination of bioinformatics predictions and literature based evidences we mined over 701 Human and 329 Zebrafish miRNAs to derive a list of 29 miRNAs targeting vascular specific genes in zebrafish. We shortlisted eight miRNAs and investigated their potential role in regulating vascular development in zebrafish transgenic model. In this screen we identified three miRNAs, namely miR-1, miR-144 and miR-142a-3p that have the potential to influence vascular development in zebrafish. We show that miR-142a-3p
http://journals.plos.org/plosone/article/figure?id=10.1371/journal.pone.0052588.g007
Expression analysis of GFAP like gene in Zebrafish embryogenesis | [email protected]
The present study focuses on a cDNA fragment of 2.6 kb coding for glial fibrillary acidic protein (GFAP) related gene in zebrafish. Earlier work in the lab revealed that this gene showed 72% homology, at the amino acid level, with the mammalian gene coding for GFAP. Due to variations observed in sequence of head domain of GFAP and in expression pattern compared to that of rodent GFAP, this gene was named as zebrafish GFAP like gene (zfgfap-l)( Gene Bank Accession No: AY 397679).In this project, the expression pattern of zfgfap-l was studied by RT-PCR and in situ hybridization. RT-PCR results showed that the expression of zfgfap-l started as early as 1.5 hours post-fertilization (hpf) and increased steadily up to 10 hpf, then became a constant level of expression till 30 hpf. In embryos at sphere stage, the expression was detected in the cells of the superficial layer by in situ hybridization. After gastrulation, the expression became restricted to the neural tube, particularly in the presumptive ...
http://scholarbank.nus.edu.sg/handle/10635/23034
A Defined Zebrafish Line for High-Throughput Genetics and Genomics: NHGRI-1 | GeneticsA Defined Zebrafish Line for High-Throughput Genetics and Genomics: NHGRI-1 | Genetics
THE zebrafish (Danio rerio) is a powerful tool for understanding vertebrate biology. The usefulness of this model organism is bolstered by the availability of a finished sequenced and annotated genome (Howe et al. 2013; Flicek et al. 2014). As a natural extension of this resource, there are several high-throughput efforts to systematically mutagenize all zebrafish protein-coding genes (Moens et al. 2008; Kettleborough et al. 2013; Varshney et al. 2013a,b).. In addition to such projects, the combination of a sequenced genome and developments in targeted nuclease technology mean that the zebrafish community is now able to rapidly take advantage of custom genome-editing technologies (Doyon et al. 2008; Bedell et al. 2012; Hruscha et al. 2013; Hwang et al. 2013; Jao et al. 2013). CRISPRs in particular provide an efficient, easy, and inexpensive means of manipulating and interrogating the genome (Jinek et al. 2012; Cong et al. 2013; Mali et al. 2013). However, because there are very few hardy ...
https://www.genetics.org/content/198/1/167?ijkey=50520c720d63fbfb229f3a16d6f2c935210603c8&keytype2=tf_ipsecsha
The zebrafish as a tool to identify novel therapies for human cardiovascular disease | Disease Models & MechanismsThe zebrafish as a tool to identify novel therapies for human cardiovascular disease | Disease Models & Mechanisms
Several cardiomyopathy models have been developed in the zebrafish. Unlike other vertebrate models, zebrafish larvae have the unique capacity to oxygenate through diffusion alone for the first 7 dpf. This allows for the study of mutations leading to severe cardiovascular compromise, including those that result in a non-contractile heart. One such mutation, silent heart, was initially identified through a forward genetic screen and subsequently attributed to disruption of the gene TNNT2, encoding the cardiac contractile protein troponin T (Chen et al., 1996; Sehnert et al., 2002; Stainier et al., 1996). Zebrafish expressing the silent heart mutation also demonstrate a reduction in α-tropomyosin and cardiac troponins C and I, resulting in severe sarcomere defects and cardiomyocyte disarray. These ultrastructural changes parallel those seen in human TNNT2 mutations, which lead to familial forms of hypertrophic and dilated cardiomyopathy. Similarly, the pickwickm171 mutation in zebrafish causes a ...
https://dmm.biologists.org/content/7/7/763
ZFIN Publication: Kalev-Zylinska et al., 2002ZFIN Publication: Kalev-Zylinska et al., 2002
RUNX1/AML1/CBFA2 is essential for definitive hematopoiesis, and chromosomal translocations affecting RUNX1 are frequently involved in human leukemias. Consequently, the normal function of RUNX1 and its involvement in leukemogenesis remain subject to intensive research. To further elucidate the role of RUNX1 in hematopoiesis, we cloned the zebrafish ortholog (runx1) and analyzed its function using this model system. Zebrafish runx1 is expressed in hematopoietic and neuronal cells during early embryogenesis. runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl, and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche. Transient expression of runx1 in cloche embryos resulted in partial rescue of the hematopoietic defect. Depletion of Runx1 with antisense morpholino oligonucleotides abrogated the development of both blood and vessels, as demonstrated by loss of circulation, incomplete development of vasculature ...
http://zfin.org/ZDB-PUB-020410-3
Single-cell reconstruction of developmental trajectories during zebrafish embryogenesisSingle-cell reconstruction of developmental trajectories during zebrafish embryogenesis
During embryogenesis, cells acquire distinct fates by transitioning through transcriptional states. To uncover these transcriptional trajectories during zebrafish embryogenesis, we sequenced 38,731 cells and developed URD, a simulated diffusion-based computational reconstruction method. URD identifi …
https://pubmed.ncbi.nlm.nih.gov/29700225/
Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation.Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation.
Huang H., Lu F.I., Jia S., Meng S., Cao Y., Wang Y., Ma W., Yin K., Wen Z., Peng J., Thisse C., Thisse B., Meng A.. Angiomotin (Amot), the founding member of the Motin family, is involved in angiogenesis by regulating endothelial cell motility, and is required for visceral endoderm movement in mice. However, little is known about biological functions of the other two members of the Motin family, Angiomotin-like1 (Amotl1) and Angiomotin-like2 (Amotl2). Here, we have identified zebrafish amotl2 as an Fgf-responsive gene. Zebrafish amotl2 is expressed maternally and in restricted cell types zygotically. Knockdown of amotl2 expression delays epiboly and impairs convergence and extension movement, and amotl2-deficient cells in mosaic embryos fail to migrate properly. This coincides with loss of membrane protrusions and disorder of F-actin. Amotl2 partially co-localizes with RhoB-or EEA1-positive endosomes and the non-receptor tyrosine kinase c-Src. We further demonstrate that Amotl2 interacts ...
http://www.uniprot.org/citations/17293535
Retinoic acid signaling regulates expression of the tandemly duplicated LWS1 and LWS2 genes in zebrafish. | IOVS | ARVO JournalsRetinoic acid signaling regulates expression of the tandemly duplicated LWS1 and LWS2 genes in zebrafish. | IOVS | ARVO Journals
Purpose: Differentiation of diverse photoreceptor phenotypes in the vertebrate retina requires multiple signaling pathways that activate cascades of gene expression. The signaling molecule retinoic acid (RA) is known to regulate rod and cone cell fate, differentiation, and survival. The purpose of the current study is to identify photoreceptor genes controlled by RA signaling in the embryonic retina of the zebrafish.. Methods: We treated embryos with RA at 48 hours post-fertilization (hpf) and isolated total RNA from eyes for microarray analysis at 75 hpf in order to identify genes responding to RA over the period of photoreceptor differentiation. Differentially expressed genes were validated by quantitative RT-PCR (qRT-PCR) and in situ hybridization. Wild-type zebrafish and those carrying an RA signaling reporter transgene (RARE:YFP) were used.. Results: We identified 180 genes with significantly altered gene expression. Of interest was the long wavelength sensitive opsin 1 (LWS1) gene, which ...
http://iovs.arvojournals.org/article.aspx?articleid=2269506
Loyola eCommons - Undergraduate Research and Engagement Symposium: Expression of mRNA of the col2a1b gene during zebrafish...Loyola eCommons - Undergraduate Research and Engagement Symposium: Expression of mRNA of the col2a1b gene during zebrafish...
We set out to characterize the expression of col2a1b, the zebrafish ortholog of the human COL2A1. In zebrafish, the col2a1a gene has been shown to be expressed in both chondrocytes and perichondrial cells, whereas our preliminary work suggests col2a1b is expressed only in the perichondrium of cartilage elements; thus, col2a1b collagen promotes cartilage development and ossification of chondrocytes. Our lab is interested in understanding the differential expression of the two homologs. In this report, we document the complete spatiotemporal expression of the zebrafish col2a1b mRNA during the first 5 days of development via in situ hybridization.
https://ecommons.luc.edu/ures/2021/2021/45/
BOC, brother of CDO, is a dorsoventral axon-guidance molecule in the embryonic vertebrate brain. | Semantic ScholarBOC, brother of CDO, is a dorsoventral axon-guidance molecule in the embryonic vertebrate brain. | Semantic Scholar
The early axon scaffolding in the embryonic vertebrate brain consists of a series of ventrally projecting axon tracts that grow into a single major longitudinal pathway connected across the midline by commissures. We have investigated the role of Brother of CDO (BOC), an immunoglobulin (Ig) superfamily member distantly related to the Roundabout (Robo) family of axon-guidance receptors, in the development of this embryonic template of axon tracts in the zebrafish brain. A zebrafish homologue of BOC was isolated and shown to be expressed predominantly in the developing neural plate and later in the neural tube and developing brain. Zebrafish boc was initially highly localized to discrete bands in the mid- and hindbrain, but, as the major brain subdivisions emerged, it became more evenly expressed along the rostrocaudal axis, particularly in dorsal regions. The function of zebrafish boc was examined by a loss-of-function approach. Analysis of embryos injected with antisense morpholinos designed against boc
https://www.semanticscholar.org/paper/BOC%2C-brother-of-CDO%2C-is-a-dorsoventral-molecule-in-Connor-Allen/91e9f3a3116c2905d4135d8d36a524f7dee844f8
Study of pronephric-glomerular morphogenesis in zebrafish
Midline convergence of organ primordia is an important mechanism for shaping the vertebrate body-plan at various stages of development, such as the morphogenesis of the heart and endoderm. Down regulation of wnt or noncanonical wnt signalling components, such as dishelleved (Dvl) or RhoA GTPase (RhoA), impairs midline convergence of the heart primordia and endoderm in zebrafish. This suggests that wnt signaling plays an important role in regulating midline convergence. At the early patterning stage of the zebrafish kidney, the two pronephric-glomerular primodia (PGP), which derive from intermediate mesoderm, converge towards the midline and fuse to form a functional pronephros. In contrast, during development of the mammalian kidney, the pronephros degenerates as the mesonephros develops without midline convergence. The hypothesis is thus that there is/are mechanisms underlying midline convergence of PGP in zebrafish, which is/are in addition to the control of the non-canonical wnt/Dvl/RhoA ...
https://www.era.lib.ed.ac.uk/handle/1842/5671
New MS Drug Candidate From Zebrafish Screen Targets IGF-1R Pathway | Multiple Sclerosis Discovery Forum
The zebrafish has emerged as a premier system for drug discovery. Several small molecules found to be active in zebrafish have been studied in clinical trials. We are at the beginning of an era in which new pathways or chemicals will rescue disease models and lead to new therapeutics, Leonard Zon, M.D., Ph.D., told MSDF. Zon is Grousbeck Professor of Pediatric Medicine at Boston Childrens Hospital and a Howard Hughes Medical Institute investigator. Trede did his postdoctoral work in Zons lab, but Zon was not involved in the LDK study.. At the University of Utah in 2004, Trede began using zebrafish to interrogate a small-molecule library in an effort to identify compounds that kill immature T cells with arrested development. This served as a stand-in for leukemia. He linked the jellyfish gene for green fluorescent protein (GFP) to a zebrafish T cell promoter gene to mark the T cells, which develop in the thymus, easily seen behind a zebrafishs eye. Whenever a T cell lights up green, it ...
https://www.msdiscovery.org/news/new_findings/10628-new-ms-drug-candidate-zebrafish-screen-targets-igf-1r-pathway
Identification of the zebrafish maternal and paternal transcriptomes | DevelopmentIdentification of the zebrafish maternal and paternal transcriptomes | Development
The development of new sequencing technologies permits the study of mRNA expression to an unprecedented level. Recent RNA-seq experiments have analysed the changing transcriptome in the early zebrafish embryo (Aanes et al., 2011; Vesterlund et al., 2011; Pauli et al., 2012), but the work described here provides the first systematic identification of maternal and paternal mRNAs in this species. Previous microarray analyses had suggested that transcription occurs before the MZT in the zebrafish (Mathavan et al., 2005) and RNA-seq experiments have also described changes in mRNA levels prior to the MZT, suggesting that such changes were due to the post-transcriptional regulation of mRNAs (Aanes et al., 2011). By identifying zygotically expressed genes (Fig. 2) and then applying tight temporal resolution (Fig. 5B,C), our work confirms that, consistent with previous radioactivity incorporation experiments (Kane and Kimmel, 1993), zygotic transcription begins after ten cell cycles. Through the ...
http://dev.biologists.org/content/140/13/2703
Zebrafish models for large-scale genetic screens in dyslipidemia and atherosclerosis : Validation and applicationZebrafish models for large-scale genetic screens in dyslipidemia and atherosclerosis : Validation and application
Hundreds of loci have been robustly associated with circulating lipids, atherosclerosis and coronary artery disease; but for most loci the causal genes and mechanisms remain uncharacterized. The overall aim of my thesis is to develop and validate novel in vivo model systems that are suitable for high-throughput, image-based genetic screens in coronary artery disease and related traits, and use these model systems to systematically characterize positional candidate genes.. In Study I, I developed an experimental pipeline to validate the suitability of zebrafish larvae as a model system for systematic, large-scale characterization of drugs and genes associated with dyslipidemia and atherosclerosis. Using this pipeline, I showed that five days of overfeeding and cholesterol supplementation have independent pro-atherogenic effects in zebrafish larvae, which could be diminished by concomitant treatment with atorvastatin and ezetimibe. CRISPR-Cas9-induced mutations in orthologues of proof-of-concept ...
http://uu.diva-portal.org/smash/record.jsf?pid=diva2%3A1372477&c=2&searchType=LIST_COMING&language=en&query=&af=%5B%5D&aq=%5B%5B%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all
Zbrafish] Antibody for SOX9a ZebrafishZbrafish] Antibody for SOX9a Zebrafish
Hi Jorge, AnaSpec sells rabbit polyclonal anti-SOX9 antibody (cat# 28138) which will work for zebrafish SOX9a. Warm regards, Daniel [Zbrafish] Antibody for SOX9a Zebrafish Jorge Soares via zbrafish%40net.bio.net (by j.s.soares from gmail.com) Wed Aug 1 08:07:04 EST 2007 a.. Next message: [Zbrafish] PH meter b.. Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] -------------------------------------------------------------------------------- Hi everyone, Just recently joined this mailing list, mainly because Im searching for an Antibody (mono or poli) that binds to SOX9a from Zebrafish, still leaving the TFBS (transcription Factor Bionding Site) open for it to be able to link to the DNA im studying. Does anyone know of a company that produces it? Cheers. Jorge -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20070801/9cfd411f/attachment.html -- I am using the free version of SPAMfighter. We are a ...
http://www.bio.net/bionet/mm/zbrafish/2008-November/006526.html
Koe-el intoiminta - Seeprakalat - Zebrafish
including: Anesthesia and Euthanasia in Zebrafish Guidance on the housing and care of zebrafish Danio Rerio 2011 Katso my s kaloista yleens : NC3Rs - Fish ...
http://www.laaninhallitus.fi/lh/etela/hankkeet/ellapro/home.nsf/pages/98D66A162D780E2AC2257B1A00253950?opendocument
Manual Drainage of the Zebrafish Embryonic Brain Ventricles | ProtocolManual Drainage of the Zebrafish Embryonic Brain Ventricles | Protocol
We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain...
https://www.jove.com/video/4243/manual-drainage-of-the-zebrafish-embryonic-brain-ventricles
Dimerized glycosaminoglycan chains increase FGF signaling during zebrafish development. | HSPG PEG
Proteoglycans (PGs) modulate numerous signaling pathways during development through binding of their glycosaminoglycan (GAG) side chains to various signaling molecules, including fibroblast growth factors (FGFs). A majority of PGs possess two or more GAG side chains, suggesting that GAG multivalency is imperative for biological functions in vivo. However, only a few studies have examined the biological significance of GAG multivalency. In this report, we utilized a library of bis- and tris-xylosides that produce two and three GAG chains on the same scaffold, respectively, thus mimicking PGs, to examine the importance of GAG valency and chain type in regulating FGF/FGFR interactions in vivo in zebrafish. A number of bis- and tris-xylosides, but not mono-xylosides, caused an elongation phenotype upon their injection into embryos. In situ hybridization showed that elongated embryos have elevated expression of the FGF target gene mkp3 but unchanged expression of reporters for other pathways, ...
http://pegnac.sdsc.edu/hspg-peg/23614643/
Frontiers | Integrating anatomy and function for zebrafish circuit analysis | Frontiers in Neural CircuitsFrontiers | Integrating anatomy and function for zebrafish circuit analysis | Frontiers in Neural Circuits
Due to its transparency, virtually every brain structure of the larval zebrafish is accessible to light-based interrogation of circuit function. Advanced stimulation techniques allow the activation of optogenetic actuators at different resolution levels, and genetically encoded calcium indicators report the activity of a large proportion of neurons in the CNS. Large datasets result and need to be analyzed to identify cells that have specific properties - e.g. activity correlation to sensory stimulation or behavior. Advances in three-dimensional functional mapping in zebrafish are promising; however the mere coordinates of implicated neurons are not sufficient. To comprehensively understand circuit function, these functional maps need to be placed into the proper context of morphological features and projection patterns, neurotransmitter phenotypes, and key anatomical landmarks. We discuss the prospect of merging functional and anatomical data in an integrated atlas from the perspective of our work on
https://www.frontiersin.org/articles/10.3389/fncir.2013.00074/full
Chemokine‐guided cell migration and motility in zebrafish development | The EMBO Journal
Recently, chemokine gradients have been directly visualized in vivo in the context of Ccl21‐mediated dendritic cell migration in mice (Weber et al, 2013) and in zebrafish, where Cxcl8 mediates neutrophil migration (Sarris et al, 2012) (Fig 2B). Cxcl8 forms an extracellular, matrix‐bound gradient that extends at least 100 μm around the cell that expresses the chemokine. Interestingly, Cxcl8 protein was detected beyond this local tissue gradient and was found to be enriched along the venous vasculature, which includes the CHT from which Cxcl8 meditates the mobilization of neutrophils into the vasculature (Sarris et al, 2012). Since Cxcl8 binding to the venous vasculature is also required for neutrophil arrest on the blood vessel wall and to facilitate the subsequent extravasation (Middleton et al, 1997), it appears that Cxcl8 acts at several stages of neutrophil recruitment to sites of infection.. Binding of Cxcl8 to the extracellular matrix, or more specifically to heparan sulfate ...
http://emboj.embopress.org/content/early/2015/03/11/embj.201490105
Zebrafish Lines at ZIRC
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http://zebrafish.org/fish/lineAll.php?Anything=sa374
Zebrafish Lines at ZIRC
Thank you for visiting zebrafish.org. Due to the Covid-19 pandemic, resource distribution has been reduced and services may be intermittent. All orders will be processed in the order received. We will contact you to make arrangements for our next available shipping date. Emergency consultations for health, husbandry, or aquaculture will be addressed as soon as possible. ...
http://zebrafish.org/fish/lineAll.php?OID=ZDB-ALT-131217-12059
Optimization of the spontaneous tail coiling test for fast assessment of neurotoxic effects in the zebrafish embryo using an...Optimization of the spontaneous tail coiling test for fast assessment of neurotoxic effects in the zebrafish embryo using an...
Mentioning: 6 - Optimization of the spontaneous tail coiling test for fast assessment of neurotoxic effects in the zebrafish embryo using an automated workflow in KNIME® - Eberhard Küster, Stefan Scholz, Riccardo Massei, Elisabet Teixidó, Afolarin O. Ogungbemi
https://scite.ai/reports/optimization-of-the-spontaneous-tail-wmXW8QlM
A transcriptomics-based hepatotoxicity comparison between the zebrafish embryo and established human and rodent in vitro and in...A transcriptomics-based hepatotoxicity comparison between the zebrafish embryo and established human and rodent in vitro and in...
The zebrafish revealed a central regulator for the development of the brain histam... ( Research has shown that mutations in.....The zebrafish revealed a central regulator for the development of the brain histam... ( Research has shown that mutations in.....
Health, ...Research has shown that mutations in the psen1 gene are common in the ...Professor Pertti Panulas research team at the University of Helsinki ...In the study the zebrafish was used as a model organism. The rapidly d...The lack of Presenilin-1 protein induced a change in the behavior of t...,The,zebrafish,revealed,a,central,regulator,for,the,development,of,the,brain,histamine,system,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
http://www.bio-medicine.org/medicine-news-1/The-zebrafish-revealed-a-central-regulator-for-the-development-of-the-brain-histamine-system-99561-1/
Atoh7 (zebrafish) blocking peptide | GeneTexAtoh7 (zebrafish) blocking peptide | GeneTex
, Atoh7 (zebrafish) blocking peptide, GTX48514-PEP, Applications: Apuri, Blocking, ELISA; Affinity purification, Blocking, ELISA; CrossReactivity: Zebrafish
http://www.genetex.com/Atoh7-zebrafish-blocking-peptide-GTX48514-PEP.html
Zebrafish primordial germ cell cultures derived from vasa::RFP transgenic embryos<...
TY - JOUR. T1 - Zebrafish primordial germ cell cultures derived from vasa::RFP transgenic embryos. AU - Fan, Lianchun. AU - Moon, Jesung. AU - Wong, Ten Tsao. AU - Crodian, Jennifer. AU - Collodi, Paul. PY - 2008/6/1. Y1 - 2008/6/1. N2 - Although embryonic germ (EG) cell-mediated gene transfer has been successful in the mouse for more than a decade, this approach is limited in other species due to the difficulty of isolating the small numbers of progenitors of germ cell lineage (PGCs) from early-stage embryos and the lack of information on the in vitro culture requirements of the cells. In this study, methods were established for the culture of PGCs obtained from zebrafish embryos. Transgenic embryos that express the red fluorescent protein (RFP) under the control of the PGC-specific vasa promoter were used, making it possible to isolate pure populations of PGCs by fluorescence-activated cell sorting (FACS) and to optimize the culture conditions by counting the number of fluorescent PGC colonies ...
https://utsouthwestern.pure.elsevier.com/en/publications/zebrafish-primordial-germ-cell-cultures-derived-from-vasarfp-tran
Zebrafish Mutation Project: Functional Genomics of Disease.Zebrafish Mutation Project: Functional Genomics of Disease.
The advent of high-throughput sequencing has greatly accelerated the identification of inherited and de novo disease causing mutations. Following discovery the analysis of the developmental and cellular pathways of the affected genes is a crucial step on the path towards therapy with model organisms as the central tool. Traditionally, vertebrate model organisms such as mouse and zebrafish have been used on a gene-by-gene basis, however, in order to keep pace with the increasing speed of discovery, new approaches are needed. Owing to a high quality genome reference sequence and its genetic and embryological tractability the zebrafish is a vertebrate model especially suited for large scale studies. Previously having established methods to generate and identify disruptive zebrafish point mutations on a genome wide scale the Zebrafish Mutation Project is now assigning biological function to every protein-coding gene in the zebrafish genome. We submit alleles to a high-throughput assessment of ...
http://www.ashg.org/2013meeting/abstracts/fulltext/f130121952.htm
Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA...Swap70b is required for convergent and extension cell movement during zebrafish gastrulation linking Wnt11 signalling and RhoA...
Swap70 functions as a guanine nucleotide exchange factor for Rac and RhoA regulating F-actin cytoskeletal rearrangements and playing a crucial role in mammalian cell activation, migration, adhesion and invasion. Here we show that the zebrafish orthologue, Swap70b, is required for convergent and extension cell movement during gastrulation. Swap70b morphants exhibited broader and shorter body axis but cell fate specification appeared normal. While ectopic Swap70b expression robustly rescued Wnt11 morphants, RhoA overexpression was sufficient to rescue Swap70b morphants, establishing Swap70b as a novel member of the non-canonical Wnt/PCP pathway downstream of Wnt11 and upstream of RhoA. This is distinct from the related Def6a protein that acts downstream of Wnt5b. Def6a/Swap70b morphants resemble Ppt/Slb double mutant embryos suggesting that Swap70b and Def6a delineate Wnt11 and Wnt5b signalling pathways.. ...
http://eprints.nottingham.ac.uk/2917/
Dna hypomethylation induces a dna replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish...
TY - JOUR. T1 - Dna hypomethylation induces a dna replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos. AU - Jacob, Vinitha. AU - Chernyavskaya, Yelena. AU - Chen, Xintong. AU - Tan, Poh Seng. AU - Kent, Brandon. AU - Hoshida, Yujin. AU - Sadler, Kirsten C.. PY - 2015/2/1. Y1 - 2015/2/1. N2 - UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication and is essential for maintaining DNA methylation. uhrf1 mutant zebrafish have global DNA hypomethylation and display embryonic defects, includinga small liver, and theydie as larvae. Wemake the surprising finding that, despite their reduced organ size, uhrf1 mutants express high levels of genes controlling S-phase and have many more cells undergoing DNA replication, as measured by BrdU incorporation. In contrast to wild-type hepatocytes, which are continually dividing during hepatic outgrowth and thus dilute the BrdU label, uhrf1 mutant ...
https://nyuscholars.nyu.edu/en/publications/dna-hypomethylation-induces-a-dna-replication-associated-cell-cyc
PLOS Genetics: Transcriptome Analysis of Zebrafish Embryogenesis Using MicroarraysPLOS Genetics: Transcriptome Analysis of Zebrafish Embryogenesis Using Microarrays
Zebrafish (Danio rerio) is a well-recognized model for the study of vertebrate developmental genetics, yet at the same time little is known about the transcriptional events that underlie zebrafish embryogenesis. Here we have employed microarray analysis to study the temporal activity of developmentally regulated genes during zebrafish embryogenesis. Transcriptome analysis at 12 different embryonic time points covering five different developmental stages (maternal, blastula, gastrula, segmentation, and pharyngula) revealed a highly dynamic transcriptional profile. Hierarchical clustering, stage-specific clustering, and algorithms to detect onset and peak of gene expression revealed clearly demarcated transcript clusters with maximum gene activity at distinct developmental stages as well as co-regulated expression of gene groups involved in dedicated functions such as organogenesis. Our study also revealed a previously unidentified cohort of genes that are transcribed prior to the mid-blastula transition,
http://journals.plos.org/plosgenetics/article/related?id=10.1371/journal.pgen.0010029
FYI: Zebrafish as a model system in neurobiology IFYI: Zebrafish as a model system in neurobiology I
Dear Zebrafish Researchers, Methods in Cell Science has a special Issue devoted to Zebrafish coming out in November at the Society for Neurosciences Annual Meeting, and entitled:Zebrafish as a model system in neurobiology I. It is a diverse Issue, documenting some of the many techniques and exciting experiments, beyond genomics, possible with Zebrafish. I edited this Issue, and the table of contents for Zebrafish as a model system in neurobiology I is shown below. Soren Andersen ---- TABLE OF CONTENTS List of contributors Editorial Soren S. L. Andersen 1) Zebrafish as an experimental model: strategies for developmental and molecular neurobiology studies Authors 2) Neural fate analysis in Zebrafish using olig2 BAC transgenics Authors 3) Neuron-specific gene manipulation in transparent Zebrafish embryos Authors 4) Practical procedures for ectopic induction of gene expression in zebrafish embryos using Bhc-diazo-caged mRNA Authors 5) Identifying axon guidance defects in the embryonic Zebrafish ...
http://www.bio.net/bionet/mm/zbrafish/2003-August/005390.html
Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo<...Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo<...
TY - JOUR. T1 - Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. AU - Tseng, Deng Yu. AU - Chou, Ming Yi. AU - Tseng, Yung Che. AU - Hsiao, Chung Der. AU - Huang, Chang Jen. AU - Kaneko, Toyoji. AU - Hwang, Pung Pung. PY - 2009/3/1. Y1 - 2009/3/1. N2 - Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca2+ uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca2+ uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater stimulated whole body Ca2+ influx and zebrafish epithelial Ca2+ channel (zECaC) mRNA expression, while downregulated ...
https://scholar.lib.ntnu.edu.tw/en/publications/effects-of-stanniocalcin-1-on-calcium-uptake-in-zebrafish-danio-r
The Zebrafish GenomeWiki: a crowdsourcing approach to connect the long tail for zebrafish gene annotation. - Radcliffe...The Zebrafish GenomeWiki: a crowdsourcing approach to connect the long tail for zebrafish gene annotation. - Radcliffe...
A large repertoire of gene-centric data has been generated in the field of zebrafish biology. Although the bulk of these data are available in the public domain, most of them are not readily accessible or available in nonstandard formats. One major challenge is to unify and integrate these widely scattered data sources. We tested the hypothesis that active community participation could be a viable option to address this challenge. We present here our approach to create standards for assimilation and sharing of information and a system of open standards for database intercommunication. We have attempted to address this challenge by creating a community-centric solution for zebrafish gene annotation. The Zebrafish GenomeWiki is a wiki-based resource, which aims to provide an altruistic shared environment for collective annotation of the zebrafish genes. The Zebrafish GenomeWiki has features that enable users to comment, annotate, edit and rate this gene-centric information. The credits for contributions
https://www.rdm.ox.ac.uk/publications/472430
advantages of zebrafish as a model organism
Naturally, the best model organism depends on the biological question, and zebrafish are not ideal for every application. They share about 70% of their genes with humans and 85% of human genes associated with a disease have a homolog in zebrafish ( Howe et al., 2013 ). The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often called a tropical fish although both tropical and subtropical).. This Primer will provide a synopsis of the different cancer models generated by the zebrafish community to date. * Why are zebrafish used in research? Zebrafish (Danio rerio) are now firmly established as a powerful research model for many areas of biology and medicine. Created by. 13 14. Model organisms advantages and disadvantages. As discussed in the previous section, a simple application of this model would be to evaluate drugs useful in ...
http://oldsilverback.com/5mcb9/0750c1-advantages-of-zebrafish-as-a-model-organism
Cells | Free Full-Text | The Functional Role of CONNEXIN 26 Mutation in Nonsyndromic Hearing Loss, Demonstrated by Zebrafish...Cells | Free Full-Text | The Functional Role of CONNEXIN 26 Mutation in Nonsyndromic Hearing Loss, Demonstrated by Zebrafish...
Nonsyndromic hearing loss (NSHL) is of great clinical importance, and mutations in the GJB2 gene and the encoded human CONNEXIN 26 (CX26) protein play important roles in the genetic pathogenesis. The CX26 p.R184Q mutation was shown to be a dominant-negative effect in our previous study. Previously, we also demonstrated that zebrafish Cx30.3 is orthologous to human CX26. In the present study, we established transgenic zebrafish models with mutated Cx30.3 specifically expressed in the supporting cells of zebrafish inner ears driven by the agr2 promoter, to demonstrate and understand the mechanism by which the human CX26 R.184 mutation causes NSHL. Our results indicated that significant structural changes in the inner ears of transgenic lines with mutations were measured and compared to wild-type zebrafish. Simultaneously, significant alterations of transgenic lines with mutations in swimming behavior were analyzed with the zebrafish behavioral assay. This is the first study to investigate the functional
https://www.mdpi.com/2073-4409/9/5/1291/htm
The development of the posterior body in zebrafish | DevelopmentThe development of the posterior body in zebrafish | Development
In order to understand the developmental mechanisms of posterior body formation in the zebrafish, a fate map of the zebrafish tailbud was generated along with a detailed analysis of tailbud cell movements. The fate map of the zebrafish tailbud shows that it contains tissue-restricted domains and is not a homogeneous blastema. Furthermore, time-lapse analysis shows that some cell movements and behaviors in the tailbud are similar to those seen during gastrulation, while others are unique to the posterior body. The extension of axial mesoderm and the continuation of ingression throughout zebrafish tail development suggests the continuation of processes initiated during gastrulation. Unique properties of zebrafish posterior body development include the bilateral distribution of tailbud cell progeny and the exhibition of different forms of ingression within specific tailbud domains. The ingression of cells in the anterior tailbud only gives rise to paraxial mesoderm, at the exclusion of axial ...
https://dev.biologists.org/content/124/4/881?ijkey=3522f78611813394cd1ad0dba164873cd21a6dec&keytype2=tf_ipsecsha
Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive,...Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive,...
In this study we set out to investigate the transcriptome dynamics of early zebrafish embryogenesis with a comprehensive experimental set-up that interroga
https://redhothealthcare.com/transcriptome-dynamics-in-early-zebrafish-embryogenesis-determined-by-high-resolution-time-course-analysis-of-180-successive-individual-zebrafish-embryos/
Antiangiogenic Cancer Drug Using the Zebrafish Model | Arteriosclerosis, Thrombosis, and Vascular BiologyAntiangiogenic Cancer Drug Using the Zebrafish Model | Arteriosclerosis, Thrombosis, and Vascular Biology
Because of its permeability to small molecules, zebrafish can be used for testing and screening of drugs affecting different biological processes.50-52 Single and multiple compounds tests can be easily and successfully achieved on both zebrafish embryos and adults.53,54 The reason for this resides in the fact that (1) a high quantity of zebrafish embryos (≈5000) can be obtained synchronously, and (2) the small size of the embryos allows them to fit in a 384-well plate and thus allows high-throughput analyses. In this way, libraries of thousands of compounds can be screened for their effects in a reasonable time frame (eg, weeks). Progresses for drug screening in zebrafish embryos are also subjected to automatic readout for phenotypic effects. An automated high-throughput platform for in vivo chemical screenings on zebrafish embryos has been developed aiming for the highest possible throughput and minimization of human error. It includes an automated method for embryo collection and ...
http://atvb.ahajournals.org/content/34/9/1846
Mouse lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation<...Mouse lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation<...
TY - JOUR. T1 - Mouse lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation. AU - Meno, Chikara. AU - Gritsman, Kira. AU - Ohishi, Sachiko. AU - Ohfuji, Yasuhisa. AU - Heckscher, Elizabeth. AU - Mochida, Kyoko. AU - Shimono, Akihiko. AU - Kondoh, Hisato. AU - Talbot, William S.. AU - Robertson, Elizabeth J.. AU - Schier, Alexander F.. AU - Hamada, Hiroshi. PY - 1999/9. Y1 - 1999/9. N2 - Mammalian lefty and zebrafish antivin form a subgroup of the TGFβ superfamily. We report that mouse mutants for lefty2 have an expanded primitive streak and form excess mesoderm, a phenotype opposite to that of mutants for the TGFβ gene nodal. Analogously, overexpression of Antivin or Lefty2 in zebrafish embryos blocks head and trunk mesoderm formation, a phenotype identical to that of mutants caused by loss of Nodal signaling. The lefty2 mutant phenotype is partially suppressed by heterozygosity for nodal. Similarly, the effects of Antivin and Lefty2 can be ...
https://einstein.pure.elsevier.com/en/publications/mouse-lefty2-and-zebrafish-antivin-are-feedback-inhibitors-of-nod-2
Zebrafish Model Organism DatabaseZebrafish Model Organism Database
ZFIN serves as the zebrafish model organism database. The long term goals for ZFIN are a) to be the community database resource for the laboratory use of zebrafish, b) to develop and support integrated zebrafish genetic, genomic and developmental information, c) to maintain the definitive reference data sets of zebrafish research information, d) to link this information extensively to corresponding data in other model organism and human databases, e) to facilitate the use of zebrafish as a model for human biology and f) to serve the needs of the research community ...
https://norecopa.no/no/inventory3rs/zebrafish-model-organism-database
Auditory sensitivity of larval zebrafish (Danio rerio) measured using a behavioral prepulse inhibition assay | Journal of...Auditory sensitivity of larval zebrafish (Danio rerio) measured using a behavioral prepulse inhibition assay | Journal of...
Zebrafish, Danio rerio (Hamilton 1822), have become a valuable model for investigating the development and molecular genetics of the vertebrate inner ear (Whitfield, 2002; Nicolson, 2005). The early development of the zebrafish inner ear is similar to that of other vertebrates (Bang et al., 2001; Whitfield et al., 2002; Riley and Phillips, 2003) and its sensory hair cells are homologous to those found in mammals (Coffin et al., 2004). Over 50 genes are known to impact the zebrafish auditory inner ear and/or vestibular system (Granato et al., 1996; Whitfield et al., 1996; Whitfield et al., 2002; Riley and Phillips, 2003; Starr et al., 2004; Nicolson, 2005) and many of these genes are conserved and affect the inner ear development and function in other vertebrates, including humans (Nicolson et al., 1998; Moorman et al., 1999; Riley and Moorman, 2000; Busch-Nentwich et al., 2004; Kappler et al., 2004; Kozlowski et al., 2005). However, unlike mammals, zebrafish develop from eggs ex utero and are ...
http://jeb.biologists.org/content/216/18/3504
Systematic characterization of small RNAome during zebrafish early developmental stages | BMC Genomics | Full TextSystematic characterization of small RNAome during zebrafish early developmental stages | BMC Genomics | Full Text
During early vertebrate development, various small non-coding RNAs (sRNAs) such as MicroRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs) are dynamically expressed for orchestrating the maternal-to-zygotic transition (MZT). Systematic analysis of expression profiles of zebrafish small RNAome will be greatly helpful for understanding the sRNA regulation during embryonic development. We first determined the expression profiles of sRNAs during eight distinct stages of early zebrafish development by sRNA-seq technology. Integrative analyses with a new computational platform of CSZ (characterization of small RNAome for zebrafish) demonstrated an sRNA class transition from piRNAs to miRNAs as development proceeds. We observed that both the abundance and diversity of miRNAs are gradually increased, while the abundance is enhanced more dramatically than the diversity during development. However, although both the abundance and diversity of piRNAs are gradually decreased, the diversity was firstly increased then
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-117
Genotyping for Single Zebrafish (Fin Clip) or Zebrafish EmbryoGenotyping for Single Zebrafish (Fin Clip) or Zebrafish Embryo
Zebrafish is increasingly used a genetic model organism in biomedical studies. This protocol provides a detailed procedure about the identification of the genotype of an adult zebrafish or a zebrafish embryo.
https://bio-protocol.org/bio101/e182
HKU Scholars Hub: The role of phospholipase C gamma 1 in primitive hematopoiesis during zebrafish developmentHKU Scholars Hub: The role of phospholipase C gamma 1 in primitive hematopoiesis during zebrafish development
Objectives: Phospholipase C (PLC) gamma 1 has been shown to mediate signal transduction of tyrosine kinases and affect function of hematopoietic cells. However, its role in hematopoiesis during embryonic development is currently unclear. In this study, we examined this issue using morpholino (MO) gene knockdown in zebrafish embryos. Methods: MO targeting at the exon-1-intron-1 junction of zebrafish PLC-γ1 was injected into embryos at the one- to four-cell stage (referred herein zPLC-γ1MO embryos). Primitive hematopoiesis was examined quantitatively by flow cytometry in Tg(gata1:GFP) embryos and by real-time quantitative polymerase chain reaction at 18 hours-post-fertilization (hpf), before the onset of circulation. The embryos were also treated with receptor inhibitors of vascular endothelial growth factor, fibroblast growth factor, and platelet-derived growth factor at 25, 1, and 30 μmol/L, respectively, from one cell until 48 hpf. Results: Erythropoiesis was reduced in zPLC-γ1MO embryos, ...
http://hub.hku.hk/handle/10722/163064
Loss of wwox expression in zebrafish embryos causes edema and alters Ca2+ dynamics [PeerJ]Loss of wwox expression in zebrafish embryos causes edema and alters Ca2+ dynamics [PeerJ]
We investigated the role of the WW domain-containing oxidoreductase (wwox) gene in the embryonic development of zebrafish, with particular emphasis on intracellular Ca2+ dynamics because Ca2+ is an important intracellular messenger. Comparisons between zebrafish wwox and human WWOX sequences identified highly conserved domain structures. wwox was expressed in developing heart tissues in the zebrafish embryo. Moreover, wwox knockdown induced pericardial edema with similarities to conditions observed in human breast cancer. The wwox knockdown embryos with the edema died within a week. High Ca2+ levels were observed at the boundary between the edema and yolk in wwox knockdown embryos.
https://peerj.com/articles/727/
Identification of RNA binding motif proteins essential for cardiovascular development | BMC Developmental Biology | Full TextIdentification of RNA binding motif proteins essential for cardiovascular development | BMC Developmental Biology | Full Text
We recently identified Rbm24 as a novel gene expressed during mouse cardiac development. Due to its tightly restricted and persistent expression from formation of the cardiac crescent onwards and later in forming vasculature we posited it to be a key player in cardiogenesis with additional roles in vasculogenesis and angiogenesis. To determine the role of this gene in cardiac development, we have identified its zebrafish orthologs (rbm24a and rbm24b), and functionally evaluated them during zebrafish embryogenesis. Consistent with our underlying hypothesis, reduction in expression of either ortholog through injection of morpholino antisense oligonucleotides results in cardiogenic defects including cardiac looping and reduced circulation, leading to increasing pericardial edema over time. Additionally, morphant embryos for either ortholog display incompletely overlapping defects in the forming vasculature of the dorsal aorta (DA), posterior caudal vein (PCV) and caudal vein (CV) which are the first blood
https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-11-62
Positional cloning of a temperature-sensitive mutant emmental reveals a role for Sly1 during cell proliferation in zebrafish...Positional cloning of a temperature-sensitive mutant emmental reveals a role for Sly1 during cell proliferation in zebrafish...
TY - JOUR. T1 - Positional cloning of a temperature-sensitive mutant emmental reveals a role for Sly1 during cell proliferation in zebrafish fin regeneration. AU - Nechiporuk, Alex. AU - Poss, Kenneth D.. AU - Johnson, Stephen L.. AU - Keating, Mark T.. N1 - Funding Information: We are grateful to the members of Stephen Johnsons lab for technical advice during the mutagenesis screen. We thank Jennifer Sheppard, Janet Finney, Ann Hillam, Lindsay Wilson, Angela Sanchez, and Soo Kim for excellent fish care. We also thank the members of the Electron Microscopy core facilities at the Department of Cell Biology, Harvard Medical School for help with transmission electron microscopy. K.D.P. was supported by a postdoctoral fellowship from the Helen Hay Whitney Foundation. S.L.J. was supported by NIH PO1 Grant HD39952.. PY - 2003/6/15. Y1 - 2003/6/15. N2 - Here, we used classical genetics in zebrafish to identify temperature-sensitive mutants in caudal fin regeneration. Gross morphological, histological, ...
https://ohsu.pure.elsevier.com/en/publications/positional-cloning-of-a-temperature-sensitive-mutant-emmental-rev-2
The catalytic activity and secretion of zebrafish RNases are essential for their in vivo function in motor neurons and...
TY - JOUR. T1 - The catalytic activity and secretion of zebrafish RNases are essential for their in vivo function in motor neurons and vasculature. AU - Ferguson, Ross. AU - Holloway, Daniel E. AU - Chandrasekhar, Anand. AU - Acharya, K Ravi. AU - Subramanian, Vasanta. PY - 2019/2/1. Y1 - 2019/2/1. N2 - Angiogenin (hANG), a member of the Ribonuclease A superfamily has angiogenic, neurotrophic and neuroprotective activities. Mutations in hANG have been found in patients with Amyotrophic lateral sclerosis (ALS). The zebrafish (Danio rerio) rnasel-1, 2 and 3 are orthologues of hANG and of these only Rnasel-1 and Rnasel-2 have been shown to be angiogenic. Herein we show that NCI-65828, a potent and specific small molecule inhibitor of hANG inhibits Rnasel-1 to a similar extent. Treatment of early zebrafish embryos with NCI-65828, or with terrein, a fungal metabolite which prevents the secretion of hANG, resulted in spinal neuron aberrations as well defects in trunk vasculature. Our detailed ...
https://researchportal.bath.ac.uk/en/publications/the-catalytic-activity-and-secretion-of-zebrafish-rnases-are-esse
Int J Dev Biol - Zebrafish development and regeneration: new tools for biomedical research
Basic research in pattern formation is concerned with the generation of phenotypes and tissues. It can therefore lead to new tools for medical research. These include phenotypic screening assays, applications in tissue engineering, as well as general advances in biomedical knowledge. Our aim here is to discuss this emerging field with special reference to tools based on zebrafish developmental biology. We describe phenotypic screening assays being developed in our own and other labs. Our assays involve: (i) systemic or local administration of a test compound or drug to zebrafish in vivo; (ii) the subsequent detection or readout of a defined phenotypic change. A positive readout may result from binding of the test compound to a molecular target involved in a developmental pathway. We present preliminary data on assays for compounds that modulate skeletal patterning, bone turnover, immune responses, inflammation and early-life stress. The assays use live zebrafish embryos and larvae as well as adult
http://www.ijdb.ehu.es/web/paper/082615sb/zebrafish-development-and-regeneration-new-tools-for-biomedical-research-
Toxicity Evaluation of New Engineered Nanomaterials in Zebrafish | IMM Container
The effect of the nanoparticles on the marine organisms, depends on their size, chemical composition, surface structure, solubility and shape. In order to take advantage from their activity, preserving the surrounding environment from a possible pollution, we are trying to trap the nanoparticles into new nanomaterials. The nanomaterials tested were synthesized proposing a ground-breaking approach by an upside-down vision of the Au/TiO2nano-system to avoid the release of nanoparticles. The system was synthesized by wrapping Au nanoparticles with a thin layer of TiO2. The non-toxicity of the nano-system was established by testing the effect of the material on zebrafish larvae. Danio rerio o zebrafish was considered a excellent model for the environmental biomonitoring of aquatic environments and the Zebrafish Embryo Toxicity Test is considered an alternative method of animal test. For this reason zebrafish larvae were exposed to different concentrations of nanoparticles of TiO2 and Au and new ...
https://www.imm.cnr.it/node/35861
The Role of Cadherin fat3 During Hair Cell Formation in Zebrafish by Abigail FuchsmanThe Role of Cadherin fat3 During Hair Cell Formation in Zebrafish by Abigail Fuchsman
Cell-cell adhesion is an essential component of a multicellular system. Cadherins are a type of molecule that are known for their role in cell adhesion (Takeichi, 1991). Fat are a class of cadherins that have been shown to be responsible in planar cell polarity and epithelial cell division (Dunne et al., 1995; Viktorinová et al., 2009). Fat has gone primarily unstudied within the zebrafish model, with only fat1 being examined for expression (Down et al., 2005). Based on the 34 cadherin repeat motif of Fat genes, predicted sequences for Fat1-4 exist on online databases. In this study, the predicted Fat genes in zebrafish were examined for gene expression patterns during early developmental stages. All genes were expressed at some point in development, confirming their transcription into RNA and no longer classifying them as predicted sequences. One putative homolog of fat3 was selected for antisense morpholino oligonucleotide knockdown. Zebrafish with the fat3 knockdown did not develop hair cells,
https://digitalcommons.bard.edu/senproj_s2012/161/
A homeobox gene essential for zebrafish notochord development | NatureA homeobox gene essential for zebrafish notochord development | Nature
The notochord is a midline mesodermal structure with an essential patterning function in all vertebrate embryos. Zebrafish floating head (flh) mutants lack a notochord, but develop with prechordal plate and other mesodermal derivatives, indicating that flh functions specifically in notochord development. We show that floating head is the zebrafish homologue of Xnot, a homeobox gene expressed in the amphibian organizer and notochord. We propose that flh regulates notochord precursor cell fate.
https://www.nature.com/articles/378150a0?error=cookies_not_supported&code=c1c006cb-59a8-4fe9-bd6b-aca49a743c4b
Morphologic Changes in Retinal Pigment Epithelium (RPE) of the Hypopigmented Zebrafish Mutant, Golden (golb1) | IOVS | ARVO...Morphologic Changes in Retinal Pigment Epithelium (RPE) of the Hypopigmented Zebrafish Mutant, Golden (golb1) | IOVS | ARVO...
Purpose:: The zebrafish mutant, golden, is hypopigmented due to a mutation in a novel cation exchanger, NCKX5 (SLC24A5). Pigmentation has been shown, at least epidemiologically, to be an important factor in age-related macular degeneration (AMD). In addition, NCKX5 has been demonstrated in human RPE, and the gene encoding NCKX5 is located on chromosome 15q21, a region that also has a major susceptibility locus associated with AMD. The aim of this study is to evaluate the morphologic features of the retina/RPE of the golden zebrafish in comparison with human AMD in order to establish a possible animal model for this disease. Methods:: Wildtype (Lyles) and mutant (golden, golb1) zebrafish (sacrificed in light) were fixed in paraformaldehyde or glutaraldehyde. Eyes were removed and investigated by light (LM) or electron microscopy (EM), respectively. 22 eyes were obtained from wildtype (wt), age range 8-34 months, and 18 from golden, age range 9-33 months. Results:: By LM, there was an obvious ...
https://iovs.arvojournals.org/article.aspx?articleid=2383493
Automated Zebrafish Chorion Removal and Single Embryo Placement: Optimizing Throughput of Zebrafish Developmental Toxicity...
The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating and chemically exposing zebrafish embryos-namely, manual placement of embryos into 96- or 384-well plates and exposure of embryos while still in the chorion, a barrier of poorly characterized permeability enclosing the developing embryo. We report the automated dechorionation of 1600 embryos at once at 4 h postfertilization (hpf) and placement of the dechorionated embryos into 96-well plates for exposure by 6 hpf. The process removed ≥95% of the embryos from their chorions with 2% embryo mortality by 24 hpf, and 2% of the embryos malformed at 120 hpf. The robotic embryo placement allocated 6-hpf embryos to 94.7% ± 4.2% of the wells in multiple 96-well trials. The rate of embryo mortality was 2.8% (43 of 1536) from robotic handling, the rate of missed wells was 1.2% (18 of 1536), and the frequency of multipicks was ,0.1%. Embryo malformations observed at 24 hpf ...
https://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/r494vm63t
Genome-wide mapping of Hif-1α binding sites in zebrafish | BMC Genomics | Full TextGenome-wide mapping of Hif-1α binding sites in zebrafish | BMC Genomics | Full Text
Here we describe the use of ChIP coupled to next-generation high-throughput sequencing to identify Hif-1α binding sites throughout the zebrafish genome in the vhl mutant. ChIP allows unbiased genome-wide coverage of the zebrafish genome to identify Hif-1α binding sites, enabling development of zebrafish as an emergent model organism for research into the hypoxic response in a physiological context [41, 42, 46, 52, 68]. The use of the vhl mutant, a zebrafish model for hypoxia, has allowed us to compare Hif-1α binding sites with gene expression changes seen in this mutant.. Microarray analysis of gene expression changes in vhl mutants compared against wild-type controls produced both expected and unexpected results. The up-regulated genes included many classical hypoxia response genes that are associated with loss of Vhl in mammalian cell lines (Additional file 1). This reinforces the idea that the hypoxic response is activated in the vhl mutant. Contrary to this, we see similar numbers of ...
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-015-2169-x
Chapter 19 Zebrafish Informatics and the ZFIN Database<...
TY - JOUR. T1 - Chapter 19 Zebrafish Informatics and the ZFIN Database. AU - Westerfield, Monte. AU - Doerry, Eckehard. AU - Kirkpatrick, Arthur E.. AU - Douglas, Sarah A.. PY - 1998/1/1. Y1 - 1998/1/1. N2 - The recent increase in the number of research studies using zebrafish to analyze vertebrate development and genetics has led to a significant information access problem. A concerted effort has begun to establish and maintain a centralized database for the zebrafish research community. Because of the dramatic increase in the amount of data and the need for more sophisticated search methods, the Web site information and other zebrafish research information have been integrated into a relational database called the Zebrafish Information Network (ZFIN). The ultimate usefulness and usability of the database depend on a careful assessment of the requirements of the users, early and continued testing of prototype interfaces by real users, and analysis of the users interactive behavior while ...
https://nau.pure.elsevier.com/en/publications/chapter-19-zebrafish-informatics-and-the-zfin-database
Skeletal biology and disease modeling in zebrafish<...Skeletal biology and disease modeling in zebrafish<...
TY - JOUR. T1 - Skeletal biology and disease modeling in zebrafish. AU - Dietrich , Kristin. AU - Fiedler, Imke. AU - Kurzyukova , Anastasia. AU - López-Delgado , Alejandra Cristina. AU - Mcgowan, Lucy M. AU - Geurtzen , Karina. AU - Hammond, Chrissy. AU - Busse, Björn AU - Knopf, Franziska. PY - 2021/3. Y1 - 2021/3. N2 - Zebrafish are teleosts (bony fish), which share with mammals a common ancestor belonging to the phylum Osteichtyes, from which their endoskeletal systems have been inherited. Indeed, teleosts and mammals have numerous genetically conserved features in terms of skeletal elements, ossification mechanisms and bone matrix components in common. Yet, differences related to bone morphology and function need to be considered when investigating zebrafish in skeletal research. In this review, we focus on zebrafish skeletal architecture with emphasis on the morphology of the vertebral column and associated anatomical structures. We provide an overview on the different ossification types ...
https://research-information.bris.ac.uk/en/publications/skeletal-biology-and-disease-modeling-in-zebrafish
Wild Sex in Zebrafish: Loss of the Natural Sex Determinant in Domesticated Strains | GeneticsWild Sex in Zebrafish: Loss of the Natural Sex Determinant in Domesticated Strains | Genetics
CONSIDERING the fundamental importance of sex for species propagation, it is surprising that primary sex-determining mechanisms are not strongly conserved among animal taxa (Bull 1983; Charlesworth 1996; Ming et al. 2011; Bachtrog et al. 2014). Closely related species or even populations of the same species can have different sex-determining mechanisms (Takehana et al. 2007; Ross et al. 2009; Kobayashi et al. 2013; Heule et al. 2014; Larney et al. 2014). Zebrafish (Danio rerio) is a popular model for studies of vertebrate development, behavior, physiology, evolution, disease, and human health (Mills et al. 2007; Seth et al. 2013; Braasch et al. 2014; Ota and Kawahara 2014; Wilkinson et al. 2014), but researchers struggle with highly variable and distorted sex ratios, and investigations into the genetic nature of zebrafish sex determination are conflicting. To help understand these issues, we conducted a population genomic study of sex determination in multiple zebrafish strains.. Zebrafish ...
http://www.genetics.org/content/198/3/1291.full
CRISPRi Knockdown and Sequence Analysis of the BHIKHARI Family of Endogenous Retroviruses in ZebrafishCRISPRi Knockdown and Sequence Analysis of the BHIKHARI Family of Endogenous Retroviruses in Zebrafish
Transposable Elements (TEs) are mobile, selfish genes that have, in some cases, been shown to provide functions for the host. A poorly characterized family of LTR retrotransposons, BHIKHARI (bik), has unusual early embryonic, cell-specific expression patterns in the zebrafish (Danio rerio). This led me to investigate their possible role in the development of the zebrafish embryo and their evolutionary history in related species. I present a successful CRISPRi knockdown experiment of bik-1, one of five subfamilies of bik, that shows evidence of the involvement of bik-1 in cell differentiation. Polymorphism analysis gave evidence that bik-1 may still be active in zebrafish while the other bik subfamilies may not be. Sequence analysis of the subfamilies suggests that all five may have arisen from a common element sometime after the divergence of the Danionella and Danio genera. Additionally, I characterize bik elements as members of the recently described Lokiretrovirus clade of retroelements ...
https://ecommons.cornell.edu/handle/1813/104204
Canada Job Postings | IZFS - International Zebrafish Society
The IZFS was formed as the successor to the Society for Zebrafish Research (SZR), an entity that provided a framework for funding and grant support for the International Conferences on Zebrafish Development and Genetics and other conferences.. © International Zebrafish Society. All Rights Reserved.. ...
https://www.izfs.org/jobboard/canada-job-postings
Faculty Collaboration Database - Critical roles of CD146 in zebrafish vascular development. Dev Dyn 2005 Jan;232(1):232-44Faculty Collaboration Database - Critical roles of CD146 in zebrafish vascular development. Dev Dyn 2005 Jan;232(1):232-44
In this report, we use zebrafish as a model system to understand the importance of CD146 in vascular development. Endothelial-specific expression of CD146 was verified by whole-mount in situ hybridization. Suppression of CD146 protein expression by antisense morpholino oligonucleotides (MO) resulted in poorly developed intersomitic vessels (ISVs). In CD146 morphants, we observed a lack of blood flow through the ISV region, despite that fluorescence microangiography showed that the ISVs were present. This finding suggests that the lumens of the developing ISVs may be too narrow for proper circulation. Additionally, remodeling of the caudal vein plexus into functional vascular tubes appeared to be affected. Suppression of CD146 protein expression resulted in a circulation shunt that caused the circulation to by-pass part of the caudal artery/vein system. The same vascular defects were recapitulated by using a second morpholino oligonucleotide. This morphant expressed a truncated CD146 protein with ...
https://fcd.mcw.edu/?search/showPublication/id/37758
Zebrafish FOXP3 is required for the maintenance of immune tolerance. - The Victor Chang Cardiac Research Institute...Zebrafish FOXP3 is required for the maintenance of immune tolerance. - The Victor Chang Cardiac Research Institute...
Regulatory T (Treg) cells play a central role in the suppression of excessive immune responses against both self and non-self antigens. The development and function of Treg cells are controlled by a master regulatory gene encoding the forkhead box P3 (FOXP3) protein in mammals. However, little is known regarding the functions of Treg cells and FOXP3 in non-mammalian vertebrates. In this study, we generated mutant zebrafish lacking a functional FOXP3 ortholog, and demonstrated a significant reduction in survival accompanied by a marked increase in inflammatory gene expression, mononuclear cell infiltration, and T cell proliferation in peripheral tissues. Our findings indicate that the zebrafish FOXP3 protein may have an evolutionally conserved role in the control of immune tolerance, illuminating the potential of the zebrafish as a novel model for investigating the development and functions of Treg cells.. ...
https://eprints.victorchang.edu.au/578/
Mutant zebrafish with extra fin bones may hold clues to how the first animals walked on land - Europe Breaking NewsMutant zebrafish with extra fin bones may hold clues to how the first animals walked on land - Europe Breaking News
The fishs development follows a very similar process to the formation one of the long bones in our arm, Akimenko says.. Both genes code for proteins that are part of a pathway that controls the activity of the so-called Hox11 proteins, Hawkins found. In mammals, these proteins guide the formation of the two bones of the forearm. In fish, Hox11 is normally suppressed by these other proteins, but when they are mutated, the body starts to build a forearm. These mutations reawakened a developmental pathway in zebrafish that was hidden, says Frank Tulenko, an evolutionary developmental biologist at Monash University.. Until now, few scientists could have guessed that vav2 and wasl play a role in bone formation. That they do is changing the paradigm on limb development and evolution, says Renata Freitas, a developmental biologist at the University of Porto who calls the study a landmark.. The genetic pathway itself is likely ancient. The fact that it exists in zebrafish, which come from a ...
https://www.europebreakingnews.net/2021/02/mutant-zebrafish-with-extra-fin-bones-may-hold-clues-to-how-the-first-animals-walked-on-land/
Functional Characterization of Protein -Tyrosine Phosphatases in Zebrafish Development using Image Analysis</em>...
TY - THES. T1 - Functional Characterization of Protein -Tyrosine Phosphatases in Zebrafish Development using Image Analysis. AU - Runtuwene, V.J.. N1 - Reporting year: 2012. PY - 2012. Y1 - 2012. M3 - PhD thesis (Proefschrift). ER - ...
https://pure.knaw.nl/portal/en/publications/functional-characterization-of-protein-tyrosine-phosphatases-in-z
Zebrafish Newsgroup Information - General Information - ZFIN Community WikiZebrafish Newsgroup Information - General Information - ZFIN Community Wiki
General information:. The zebrafish newsgroup is a moderated, online discussion group for anyone interested in zebrafish research. Posted messages will first go to the newsgroups moderator (currently Jonathan Knight at ZFIN). If deemed appropriate for the newsgroup, the message will be forwarded to the full subscription list via e-mail and posted on the newsgroup site. Please note that the newsgroup is not monitored evenings or weekends, so messages posted outside of normal business hours in the Pacific time zone in the US will be delayed until the next business day.. Recent topics on the newsgroup include protocols and techniques, water quality and disease issues, and information on suppliers, job openings and funding opportunities. We encourage you to get connected with your colleagues through the zebrafish newsgroup!. Subscribing and Participating. Google Groups is a convenient way to access the zebrafish newsgroup on the web. See this URL: ...
https://wiki.zfin.org/display/general/Zebrafish+Newsgroup+Information
Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreasLnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas
Won M, Ro H, Dawid IB. Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas. Proc Natl Acad Sci U S A. 2015 Sep
https://myresearchnews.forumotion.com/t4885-lnx2-ubiquitin-ligase-is-essential-for-exocrine-cell-differentiation-in-the-early-zebrafish-pancreas
The Zebrafish Information Network (ZFIN) - Clean Research
The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism. ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.. ...
https://cleanresearch.org/science/the-zebrafish-information-network-zfin/
Genome-wide analysis of the zebrafish ETS family identifies three genes required for hemangioblast differentiation or...
ETS domain transcription factors have been linked to hematopoiesis, vasculogenesis, and angiogenesis. However, their biological functions and the mechanisms of action, remain incompletely understood. Here, we have performed a systematic analysis of zebrafish ETS domain genes and identified 31 in the genome. Detailed gene expression profiling revealed that 12 of them are expressed in blood and endothelial precursors during embryonic development. Combined with a phylogenetic tree assay, this suggests that some of the coexpressed genes may have redundant or additive functions in these cells. Loss-of-function analysis of 3 of them, erg, fli1, and etsrp, demonstrated that erg and fli1 act cooperatively and are required for angiogenesis possibly via direct regulation of an endothelial cell junction molecule, VE-cadherin, whereas etsrp is essential for primitive myeloid/endothelial progenitors (hemangioblasts) in zebrafish. Taken together, these results provide a global view of the ETS genes in the zebrafish
https://www.cardioscience.ox.ac.uk/publications/106541
rna meaning - ZF BioLabs S.L. Madrid, Zebrafish Toxicology Researchrna meaning - ZF BioLabs S.L. Madrid, Zebrafish Toxicology Research
The zebrafish (Danio rerio) is an rising mannequin system in a number of analysis areas worldwide, particularly within the International South. On this context, the current examine revised the historic use and traits of zebrafish as experimental fashions in Brazil. The information regarding the bibliometric parameters, analysis areas, geographic distribution, experimental design, zebrafish pressure, and reporter traces, in addition to current advances have been.... ...
https://www.zfbiolabs.com/tag/rna-meaning/
test kits for mold - ZF BioLabs S.L. Madrid, Zebrafish Toxicology Researchtest kits for mold - ZF BioLabs S.L. Madrid, Zebrafish Toxicology Research
The zebrafish (Danio rerio) is an rising mannequin system in a number of analysis areas worldwide, particularly within the International South. On this context, the current examine revised the historic use and traits of zebrafish as experimental fashions in Brazil. The information regarding the bibliometric parameters, analysis areas, geographic distribution, experimental design, zebrafish pressure, and reporter traces, in addition to current advances have been.... ...
https://www.zfbiolabs.com/tag/test-kits-for-mold/
Tohumluk CreekTohumluk Creek
"Dietary Protein Requirements of Zebrafish (Dania rerio)". Journal of Limnology and Freshwater Fisheries Research. 5 (1): 34-40 ...
https://en.wikipedia.org/wiki/Tohumluk_Creek
NeurogenomicsNeurogenomics
Tallafuss, Alexandra; Trepman, Alissa; Eisen, Judith S. (2009-12-01). "DeltaA mRNA and protein distribution in the zebrafish ... This can be a gene co-expression network, or a protein-protein interaction (PPI) network. The nodes of the network will be ... Several of these protein disrupting mutations have been able to be identified only with the aid of whole genome sequencing ... The zebrafish model system has already been used to study neuroregeneration and severe polygenic human diseases like cancer and ...
https://en.wikipedia.org/wiki/Neurogenomics
ThalamusThalamus
"Patterning the zebrafish diencephalon by the conserved zinc-finger protein Fezl". Development. 134 (1): 127-36. doi:10.1242/dev ... In zebrafish, it was shown that the expression of two SHH genes, SHH-a and SHH-b (formerly described as twhh) mark the MDO ... "Hedgehog signalling from the zona limitans intrathalamica orchestrates patterning of the zebrafish diencephalon". Development. ...
https://en.wikipedia.org/wiki/Thalamus
Germline developmentGermline development
... the PGCs express two CXCR4 transmembrane receptor proteins. The signaling system involving this protein and its ligand, Sdf1, ... Regardless of PGC injection site, PGCs are able to correctly migrate to their target sites.[citation needed] In zebrafish, ... Bone morphogenetic protein 4 (BMP4) is released by the extra-embryonic ectoderm (ExE) at embryonic day 5.5 to 5.75 directly ... December 2017). "The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation". ...
https://en.wikipedia.org/wiki/Germline_development
Bruno ReversadeBruno Reversade
Kelly, Gregory M; Reversade, Bruno (1997). "Characterization of a cDNA encoding a novel band 4.1-like protein in zebrafish". ... BRAWNIN In 2020, he participated in the characterization of C12orf73, a protein-coding gene responsible for the making of a 71 ... In 2005, Reversade and De Robertis detailed how multiple extracellular proteins allow embryos that are cut in two to self- ... This small peptide is essential for respiratory chain complex III (CIII) assembly in human cells and zebrafish. C2orf69 In 2021 ...
https://en.wikipedia.org/wiki/Bruno_Reversade
Roman DziarskiRoman Dziarski
"Zebrafish Peptidoglycan Recognition Proteins Are Bactericidal Amidases Essential for Defense against Bacterial Infections". ... He determined the role of DNA synthesis, intracellular calcium, protein kinase C, and inhibitory G proteins in peptidoglycan- ... "Peptidoglycan Recognition Proteins Are a New Class of Human Bactericidal Proteins". Journal of Biological Chemistry. 281 (9): ... "Peptidoglycan recognition proteins kill bacteria by activating protein-sensing two-component systems". Nature Medicine. 17 (6 ...
https://en.wikipedia.org/wiki/Roman_Dziarski
Dan LarhammarDan Larhammar
Using zebrafish, Larhammar explores the localization and functions of the memory proteins. His team works on discovering at ... mRNA production of protein, but did not block its own species' mRNA. The inhibiting substance however did not prevent protein ... Each vole species was found to have a substance that blocked the production of protein from the other two species' ... As of 2021 Larhammar leads a research team at Uppsala that studies receptors for brain neurotransmitters and uses zebrafish as ...
https://en.wikipedia.org/wiki/Dan_Larhammar
Guanylate cyclaseGuanylate cyclase
"Differential calcium signaling by cone specific guanylate cyclase-activing proteins from the zebrafish retina". PLOS ONE. 6 (8 ... In addition, studies have shown that zebrafish express a higher number of GCAPs than mammals, and that zebrafish GCAPs can bind ... Depending on cell type, it can drive adaptive/developmental changes requiring protein synthesis. In smooth muscle, cGMP is the ... Low concentrations of calcium cause the dimerization of RETGC-1 proteins through stimulation from guanylate cyclase-activating ...
https://en.wikipedia.org/wiki/Guanylate_cyclase
PLEKHA7PLEKHA7
... a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish". Disease Models & ... The protein PDZD11 was identified as a protein interacting through its N-terminal region with the N-terminal WW domain of ... The protein was discovered in Masatoshi Takeichi's lab while looking for potential binding partners for the N-terminal region ... Tille JC, Ho L, Shah J, Seyde O, McKee TA, Citi S (2015-01-01). "The Expression of the Zonula Adhaerens Protein PLEKHA7 Is ...
https://en.wikipedia.org/wiki/PLEKHA7
Microphthalmia-associated transcription factorMicrophthalmia-associated transcription factor
... "nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate". Development. ... Protein kinase C-interacting protein 1 (PKC1) associates with MITF. Their association is reduced upon cell activation. When ... Microphthalmia-associated transcription factor also known as class E basic helix-loop-helix protein 32 or bHLHe32 is a protein ... Most transcription factors function in cooperation with other factors by protein-protein interactions. Association of MITF with ...
https://en.wikipedia.org/wiki/Microphthalmia-associated_transcription_factor
Magnesium transporter1 familyMagnesium transporter1 family
Morpholino knockdown of MagT1 and TUSC3 protein expression in zebrafish embryos resulted in early developmental arrest; excess ... Protein families, Membrane proteins, Transmembrane proteins, Transmembrane transporters, Transport proteins, Integral membrane ... They designated this protein, MagT1. MagT1 is expressed as a 335 amino acid polypeptide which includes five transmembrane ... Knockdown of either MagT1 or TUSC3 protein lowered the total and free intracellular Mg2+concentrations in mammalian cell lines ...
https://en.wikipedia.org/wiki/Magnesium_transporter1_family
C6orf58C6orf58
... is tied to liver development in an orthologous protein in zebrafish. The function of the human UPF0762 is not yet well ... DUF781 is the singular domain of the protein and spans 318 of the protein's 330 amino acids. DUF781 has been linked to liver ... "liver-enriched gene 1a and 1b encode novel secretory proteins essential for normal liver development in zebrafish". PLOS ONE. 6 ... "Analysis of vaccinia virus-host protein-protein interactions: validations of yeast two-hybrid screenings". J. Proteome Res. 8 ( ...
https://en.wikipedia.org/wiki/C6orf58
CSNK1DCSNK1D
"Biochemical and cellular characteristics of the four splice variants of protein kinase CK1alpha from zebrafish (Danio rerio)". ... The AD hallmark proteins tau in NFTs or GVBs and TAR DNA-binding protein of 43 kDa (TDP-43) in GVBs colocalize with CK1δ. In ... So far, C-terminal phosphorylation of CK1δ by upstream kinases has been confirmed for protein kinase A (PKA), protein kinase B ... Locasale JW, Shaw AS, Chakraborty AK (August 2007). "Scaffold proteins confer diverse regulatory properties to protein kinase ...
https://en.wikipedia.org/wiki/CSNK1D
Ohad BirkOhad Birk
"Mutations in the microtubule-associated protein MAP11 (C7orf43) cause microcephaly in humans and zebrafish". Brain: A Journal ... "The 60-kDa heat shock protein modulates allograft rejection". Proceedings of the National Academy of Sciences of the United ... A microcephaly syndrome caused by mutations in the microtubule-associated protein MAP11 (C7orf43, TRAPPC14, MCPH25). ...
https://en.wikipedia.org/wiki/Ohad_Birk
FXYD familyFXYD family
Two other family members that are not obvious orthologs of any identified mammalian FXYD protein exist in zebrafish. All these ... The FXYD protein family is a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning ... This article incorporates text from the public domain Pfam and InterPro: IPR000272 (Protein domains, Protein families, Single- ... FXYD proteins appear to preferentially associate with Na,K-ATPase alpha1-beta isozymes, and affect their function in a way that ...
https://en.wikipedia.org/wiki/FXYD_family
Stereocilia (inner ear)Stereocilia (inner ear)
In this study, scientists used zebrafish to examine the motion of proteins within live ear cells using a confocal microscope. ... Fascin 2b, a protein involved in actin cross-linking, moves even faster. Constant movement of proteins within cells, along with ... This has shown that proteins in stereocilia move quickly, indicating that the movement of the proteins within the hair cells ... The fast movement of these proteins has changed our understanding of stereocilia and indicates that proteins within stereocilia ...
https://en.wikipedia.org/wiki/Stereocilia_(inner_ear)
WNT8BWNT8B
It encodes a protein showing 95%, 86%, and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, ... Protein Wnt-8b is a protein that in humans is encoded by the WNT8B gene. The WNT gene family consists of structurally related ... These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate ... Smolich BD, McMahon JA, McMahon AP, Papkoff J (1994). "Wnt family proteins are secreted and associated with the cell surface". ...
https://en.wikipedia.org/wiki/WNT8B
NWD1NWD1
It was originally identified during a search for immune system genes in zebrafish as a protein coding sequence related to APAF1 ... NWD1, short for NACHT and WD repeat domain containing 1, is a gene found in vertebrates, which encodes a protein that contains ... Correa RG, Krajewska M, Ware CF, Gerlic M, Reed JC (2014). "The NLR-related protein NWD1 is associated with prostate cancer and ... "Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish". Genome Biol. ...
https://en.wikipedia.org/wiki/NWD1
Genetically modified animalGenetically modified animal
Several groups have been developing zebrafish to detect pollution by attaching fluorescent proteins to genes activated by the ... There is also potential to use the silk producing machinery to make other valuable proteins. Proteins expressed by silkworms ... Human proteins expressed in mammals are more likely to be similar to their natural counterparts than those expressed in plants ... Human alpha-1-antitrypsin is another protein that is used in treating humans with this deficiency. Another area is in creating ...
https://en.wikipedia.org/wiki/Genetically_modified_animal
GATD3AGATD3A
... with homology to an Escherichia coli and a zebrafish protein that maps to chromosome 21q22.3". Hum Genet. 99 (5): 616-23. doi: ... 1996). "Isolation of cDNA for a novel human protein KNP-I that is homologous to the E. coli SCRP-27A protein from the ... Glutamine amidotransferase-like class 1 domain-containing protein 3A, mitochondrial is a protein that in humans is encoded by ... 1993). "Human liver protein map: a reference database established by microsequencing and gel comparison". Electrophoresis. 13 ( ...
https://en.wikipedia.org/wiki/GATD3A
Genetically modified organismGenetically modified organism
Several groups have been developing zebrafish to detect pollution by attaching fluorescent proteins to genes activated by the ... The majority of these products are human proteins for use in medicine. Many of these proteins are impossible or difficult to ... There is also potential to use the silk producing machinery to make other valuable proteins. Proteins currently developed to be ... Human proteins expressed in mammals are more likely to be similar to their natural counterparts than those expressed in plants ...
https://en.wikipedia.org/wiki/Genetically_modified_organism
Mir-430 microRNA precursor familyMir-430 microRNA precursor family
"The TATA-binding protein regulates maternal mRNA degradation and differential zygotic transcription in zebrafish". The EMBO ... Giraldez AJ, Mishima Y, Rihel J, Grocock RJ, Van Dongen S, Inoue K, Enright AJ, Schier AF (April 2006). "Zebrafish MiR-430 ... Thatcher EJ, Bond J, Paydar I, Patton JG (May 2008). "Genomic organization of zebrafish microRNAs". BMC Genomics. 9: 253. doi: ... Schier AF, Giraldez AJ (2006). "MicroRNA function and mechanism: insights from zebra fish". Cold Spring Harbor Symposia on ...
https://en.wikipedia.org/wiki/Mir-430_microRNA_precursor_family
Phylotypic stagePhylotypic stage
In zebrafish, the interconnectivity of proteins over developmental time was found to be highest in early development, ... re-analyzed the zebrafish dataset published by Domazet-Lošo and Tautz. They found that applying a log-transformation to the ... In zebrafish, as well as in additional transcriptomic datasets of Drosophila, the mosquito Anopheles and the nematode ... Gene knockout experiments from mice and zebrafish demonstrated that the ratio of essential genes to non-essential genes ...
https://en.wikipedia.org/wiki/Phylotypic_stage
Dock180Dock180
"A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion ... Subsequently it was reported that Dock180 was able to activate the small GTP-binding protein (G protein) Rac1 and this was ... GEFs activate G proteins by promoting this nucleotide exchange. Dock180 and related proteins differ from other GEFs in that ... which contribute to cellular signalling events by activating small G proteins. In their resting state G proteins are bound to ...
https://en.wikipedia.org/wiki/Dock180
Hereditary sensory and autonomic neuropathy type IHereditary sensory and autonomic neuropathy type I
The enzyme is implicated in the trafficking and signaling of type I bone morphogenetic protein (BMP) receptors in zebra fish ( ... The gene encodes SPTLC1 protein, which together with SPTLC2 protein, forms serine palmitoyltransferase (SPT) in humans. SPT is ... The gene encodes SPTLC2 protein which is one of two subunits of SPT. As mutations in the gene affect the same enzyme as those ... The protein also has de novo DNA methyltransferase activity which is responsible for establishing methylation patterns during ...
https://en.wikipedia.org/wiki/Hereditary_sensory_and_autonomic_neuropathy_type_I
Dock5Dock5
"A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion ... which function as activators of small G proteins. Dock5 is predicted to activate the small G protein Rac. Dock5 shares ... Meller N, Merlot S, Guda C (2005). "CZH proteins: a new family of Rho-GEFs". J. Cell Sci. 118 (Pt 21): 4937-46. doi:10.1242/jcs ... Côté JF, Vuori K (2007). "GEF what? Dock180 and related proteins help Rac to polarize cells in new ways". Trends Cell Biol. 17 ...
https://en.wikipedia.org/wiki/Dock5
AHCYL1AHCYL1
"Suppression and overexpression of adenosylhomocysteine hydrolase-like protein 1 (AHCYL1) influences zebrafish embryo ... 2006). "IRBIT, an inositol 1,4,5-trisphosphate receptor-binding protein, specifically binds to and activates pancreas-type Na+/ ... receptor-binding protein, is released from the IP3 receptor upon IP3 binding to the receptor". J Biol Chem. 278 (12): 10602-12 ...
https://en.wikipedia.org/wiki/AHCYL1
Selenoprotein PSelenoprotein P
This article incorporates text from the public domain Pfam and InterPro: IPR007672 (Protein pages needing a picture, Protein ... Kryukov GV; Gladyshev VN (December 2000). "Selenium metabolism in zebrafish: multiplicity of selenoprotein genes and expression ... This particular protein contains two domains: the C terminal and N terminal domain. The N-terminal domain is larger than the C ... Fujii M; Saijoh K; Kobayashi T; Fujii S; Lee MJ; Sumino K (October 1997). "Analysis of bovine selenoprotein P-like protein gene ...
https://en.wikipedia.org/wiki/Selenoprotein_P
GiantinGiantin
"The Golgi matrix protein giantin is required for normal cilia function in zebrafish". Biology Open. 6 (8): 1180-1189. doi: ... 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi: ... 2002). "Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein ... "The Golgi matrix protein giantin is required for normal cilia function in zebrafish". Biology Open. 6 (8): 1180-1189. doi: ...
https://en.wikipedia.org/wiki/Giantin
Proton-coupled folate transporterProton-coupled folate transporter
... and zebrafish (AAH77859) proteins. Structurally, there are twelve transmembrane helices with the N- and C- termini directed to ... PCFT is located on chromosome 17q11.2 and consists of five exons encoding a protein with 459 amino acids and a MW of ~50kDa. ... The proton-coupled folate transporter is a protein that in humans is encoded by the SLC46A1 gene. The major physiological roles ... Sharma S, Dimasi D, Bröer S, Kumar R, Della NG (April 2007). "Heme carrier protein 1 (HCP1) expression and functional analysis ...
https://en.wikipedia.org/wiki/Proton-coupled_folate_transporter
Strømme syndromeStrømme syndrome
The syndrome is caused by mutations in both copies of the CENPF gene, which codes for centromere protein F. This protein is ... Hydrocephalus was also observed in zebrafish whose CENPF genes were experimentally knocked out. Cerebellar hypoplasia in ... CENPF codes for centromere protein F. Centromere proteins are involved in the separation of chromosomes during cell division. ... Microtubules are protein structures that are part of the cytoskeleton and are necessary for cells to have diverse, complex ...
https://en.wikipedia.org/wiki/Strømme_syndrome
TENM3TENM3
The proteins were called Ten-ms in zebrafish, teneurins in chicken, Ten-m1-4, Odz1-4, Ten-m/Odz1-4, DOC4 in mouse, neurestin in ... Ten-m3 protein is expressed in the Purkinje's cell zone, molecular and granular layers and the white matter of the cerebellum. ... in 1999 from the original name, Ten-a, and the major site of the protein expression being in the nervous system. Like the Ten-m ... The 4 cysteine residues assist in protein folding, however, they are absent in Ten-m2 and Ten-m3. There are 41 amino acids in ...
https://en.wikipedia.org/wiki/TENM3
SKIV2L2SKIV2L2
Raijmakers R, Noordman YE, van Venrooij WJ, Pruijn GJ (2002). "Protein-protein interactions of hCsl4p with other human exosome ... "Mutations in gfpt1 and skiv2l2 cause distinct stage-specific defects in larval melanocyte regeneration in zebrafish". PLOS ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ... 2002). "AU binding proteins recruit the exosome to degrade ARE-containing mRNAs". Cell. 107 (4): 451-64. doi:10.1016/S0092-8674 ...
https://en.wikipedia.org/wiki/SKIV2L2
ZTTK syndromeZTTK syndrome
The SON protein is mainly localised to nuclear speckles and involved in a variety of cellular processes such as transcription, ... The consequence of SON haploinsufficiency on embryonic development has also been studied in zebrafish animal models (Danio ... The majority of SON variants found in ZTTK syndrome individuals are localised to exon 3. The SON gene encodes the SON protein, ... SON is a large protein consisting of 2426 amino acids and repeat sequences. SON is located within the human chromosomal region ...
https://en.wikipedia.org/wiki/ZTTK_syndrome
Congenital mirror movement disorderCongenital mirror movement disorder
... recombination protein A). DCC encodes a receptor for NTN1 (netrin-1), a protein thought to be responsible for axon guidance and ... "Mirror movement-like defects in startle behavior of zebrafish dcc mutants are caused by aberrant midline guidance of identified ... and thereby omission of 28 amino acids from DNAL4 protein. This mutant DNAL4 protein, in turn, could lead to faulty cross- ...
https://en.wikipedia.org/wiki/Congenital_mirror_movement_disorder
Spinal cordSpinal cord
Meanwhile, the overlying ectoderm secretes bone morphogenetic protein (BMP). This induces the roof plate to begin to secrete ... Than-Trong, Emmanuel; Bally-Cuif, Laure (2015-08-01). "Radial glia and neural progenitors in the adult zebrafish central ...
https://en.wikipedia.org/wiki/Spinal_cord
Short interspersed nuclear elementShort interspersed nuclear element
Furthermore, SINEs frequently contain motifs for YY1 polycomb proteins. YY1 is a zinc-finger protein that acts as a ... One such study examined the correlation between a certain family of SINEs with microRNAs (in zebrafish). The specific family of ... Thereafter, one of the strands is incorporated into a multi-protein RNA-induced silencing complex (RISC). Among these proteins ... encodes a protein which binds to RNA and acts as a chaperone to facilitate and maintain the LINE protein-RNA complex structure ...
https://en.wikipedia.org/wiki/Short_interspersed_nuclear_element
LECT2LECT2
... signaling protein. LECT2 has been detected in the blood and other tissues in a wide range of animal species from zebrafish to ... The secreted protein consists of 133`amino acids (mouse Lect2 consists of two varieties a typical 151 amino acid protein and an ... The protein was detected in and purified from cultures of Phytohaemagglutinin-activated human T-cell leukemia SKW-3 cells. ... LECT2 protein is widely expressed in vascular tissues, smooth muscle cells, adipocytes, cerebral neurons, apical squamous ...
https://en.wikipedia.org/wiki/LECT2
IsodesmosineIsodesmosine
"Differential expression of two tropoelastin genes in zebrafish". Matrix Biology. 26 (2): 115-124. doi:10.1016/j.matbio.2006.09. ... is an isomeric pyridinium-based amino acid resulting from the condensation of four lysine residues between elastin proteins by ...
https://en.wikipedia.org/wiki/Isodesmosine
GLI2GLI2
Zinc finger protein GLI2 also known as GLI family zinc finger 2 is a protein that in humans is encoded by the GLI2 gene. The ... reporter gene expression induced by these elements correlates with previously reported endogenous gli2 expression in zebrafish ... Gli2+protein at the US National Library of Medicine Medical Subject Headings (MeSH) GLI2+protein,+human at the US National ... The anti-apoptotic protein BCL-2 is up regulated by Gli2 and, to a lesser extent, Gli1 - but not Gli3, which may lead to ...
https://en.wikipedia.org/wiki/GLI2
TEAD3TEAD3
... +protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) v t e (Articles with short ... Mann CJ, Osborn DP, Hughes SM (Oct 2007). "Vestigial-like-2b (VITO-1b) and Tead-3a (Tef-5a) expression in zebrafish skeletal ... Translation of this protein is initiated at a non-AUG (AUA) start codon. GRCh38: Ensembl release 89: ENSG00000007866 - Ensembl ... Transcriptional enhancer factor TEF-5 is a protein that in humans is encoded by the TEAD3 gene. This gene product is a member ...
https://en.wikipedia.org/wiki/TEAD3
AP-1 transcription factorAP-1 transcription factor
Masuda A, Yoshikai Y, Kume H, Matsuguchi T (November 2004). "The interaction between GATA proteins and activator protein-1 ... light-dependent activation of Fos/AP-1 in zebrafish controls CRY-1a and WEE-1". Proceedings of the National Academy of Sciences ... "Human cytomegalovirus IE1 protein activates AP-1 through a cellular protein kinase(s)". The Journal of General Virology. 80 ( ... However, Fos proteins do not dimerize with each other and therefore can only bind to DNA when bound with Jun. The Jun-Fos ...
https://en.wikipedia.org/wiki/AP-1_transcription_factor
CereblonCereblon
... is a protein that in humans is encoded by the CRBN gene. The gene that encodes the cereblon protein is found on the ... Mahony C, Erskine L, Niven J, Greig NH, Figg WD, Vargesson N (2013). "Pomalidomide is nonteratogenic in chicken and zebrafish ... CRBN protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) Overview of all the structural ... As they report in the current issue of Science, a protein known as cereblon latched on tightly to the thalidomide. "Thalidomide ...
https://en.wikipedia.org/wiki/Cereblon
AequorinAequorin
Since then, the protein has been widely used in many model biological systems, including zebrafish, rats, mice, and cultured ... "Prediction of EF-hand calcium-binding proteins and analysis of bacterial EF-hand proteins". Proteins. 65 (3): 643-655. doi: ... Notably, the protein contains three EF hand motifs that function as binding sites for Ca2+ ions. The protein is a member of the ... In the animal, the protein occurs together with the green fluorescent protein to produce green light by resonant energy ...
https://en.wikipedia.org/wiki/Aequorin
Nuclear receptorNuclear receptor
... to nuclear receptors induces a conformation of the receptor that preferentially binds coactivator proteins. These proteins ... Schaaf MJ (2017). "Nuclear receptor research in zebrafish". Journal of Molecular Endocrinology. 59 (1): R65-R76. doi:10.1530/ ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-1178. Bibcode: ... Additional proteins including RNA polymerase are then recruited to the NR/DNA complex that transcribe DNA into messenger RNA. ...
https://en.wikipedia.org/wiki/Nuclear_receptor
DLX gene familyDLX gene family
In higher fishes like the zebrafish, there are two additional DLX genes, dlx2b (dlx5) and dlx4a (dlx8). These additional genes ... It has been suggested that DLX promotes the migration of interneurons by repressing a set of proteins that are normally ...
https://en.wikipedia.org/wiki/DLX_gene_family
PicornavirusPicornavirus
The rest of the genome encodes structural proteins at the 5' end and nonstructural proteins at the 3' end in a single ... Zebrafish. 16 (3): 291-299. doi:10.1089/zeb.2018.1710. PMID 30939077. S2CID 92999901. Ryu WS (2016). "Chapter 11 - Picornavirus ... and 1D proteins are the capsid proteins VP4, VP2, VP3, and VP1, respectively. Virus-coded proteases perform the cleavages, some ... Precursor proteins also have an effect on VPg-CRE specificity and stability. The upper RNA stem loop, to which VPg binds, has a ...
https://en.wikipedia.org/wiki/Picornavirus
MitogenMitogen
TGF-𝝱 works by binding to cell-surface receptors and activating the Smad gene regulatory proteins. Smad proteins then trigger ... For example, in zebrafish, an endogenous mitogen Nrg1 is produced in response to indications of heart damage. When it is ... One of the most important of these is TP53, a gene which produces a family of proteins known as p53. It, combined with the Ras ... The protein kinase domain found on mitogenic receptors is often hyperactivated in cancer cells, remaining turned on even in the ...
https://en.wikipedia.org/wiki/Mitogen
Dendritic cellDendritic cell
An example of this includes the interaction of the membrane proteins of the B7 family of the dendritic cell with CD28 present ... Dendritic cells have been found in rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) but their role is still not ... This communication can take the form of direct cell-cell contact based on the interaction of cell-surface proteins. ... Immature dendritic cells phagocytose pathogens and degrade their proteins into small pieces and upon maturation present those ...
https://en.wikipedia.org/wiki/Dendritic_cell
Function-spacer-lipid Kode constructFunction-spacer-lipid Kode construct
... hyaluronic acid oligomers and sialic acid residues Peptide/protein - ranging from single amino acids to proteins as large as ... Zebrafish kodecytes have been used to determine real time in vivo cell migration. Kodecytes have been used to create influenza ... Toxicity/vitality experiments in small laboratory animals, zebrafish, cell cultures, spermatozoa and embryos find no toxic ... CS1 errors: missing periodical, Biochemistry, Biotechnology, Laboratory techniques, Molecular biology techniques, Protein ...
https://en.wikipedia.org/wiki/Function-spacer-lipid_Kode_construct
BioGRIDBioGRID
The BioGRID's original focus was on curation of binary protein-protein and genetic interactions, but has expanded over several ... zebrafish) Dictyostelium discoideum AX4 (slime mold) Drosophila melanogaster (fruit fly) Emericella nidulans FGSC A4 Equus ... The Biological General Repository for Interaction Datasets (BioGRID) is a curated biological database of protein-protein ... A comprehensive biomedical resource of curated protein, genetic, and chemical interactions". Protein Science. 30 (1): 187-200. ...
https://en.wikipedia.org/wiki/BioGRID
SomatostatinSomatostatin
Zebrafish have all six. The six different genes, along with the five different somatostatin receptors, allow somatostatin to ... Barnea A, Roberts J, Ho RH (January 1999). "Evidence for a synergistic effect of the HIV-1 envelope protein gp120 and brain- ... The binding of somatostatin to its receptor in the pancreas leads to activation of an inhibitory G protein, or Gi, structurally ... In the stomach, somatostatin acts directly on the acid-producing parietal cells via a G-protein coupled receptor (which ...
https://en.wikipedia.org/wiki/Somatostatin
Aorta-gonad-mesonephrosAorta-gonad-mesonephros
Notch1 is another protein which has been implicated in the signalling pathway for HSC production. Notch1 knockouts exhibit ... Time lapse imaging of live zebrafish embryos has provided the visualisation of haematogenic endothelium differentiating into ... and non-mammalian vertebrates such as birds and zebrafish. It contains the dorsal aorta, genital ridges and mesonephros and ... "Ventral embryonic tissues and Hedgehog proteins induce early AGM hematopoietic stem cell development". Development. 136 (15): ...
https://en.wikipedia.org/wiki/Aorta-gonad-mesonephros
ChordinChordin
"Not.S - Xnot protein - Xenopus laevis (African clawed frog) - not.S gene & protein". Larraín J, Bachiller D, Lu B, Agius E, ... Experiments with zebrafish showed that a chordin gene mutation can lead to less neural and dorsal tissue. Target gene deletions ... Chordin dorsalizes the developing embryo by binding ventralizing TGFβ proteins such as bone morphogenetic proteins (BMP) ... There are five named isoforms of this protein that are produced by alternative splicing. CHRD is 23 exons long and has a length ...
https://en.wikipedia.org/wiki/Chordin
Bioorthogonal chemistryBioorthogonal chemistry
Hydrophobicity of the cyclooctyne promotes sequestration by membranes and serum proteins, reducing bioavailable concentrations ... live zebrafish, and mice. The classic copper-catalyzed azide-alkyne cycloaddition has been an extremely fast and effective ... proteins, and lipids in real time in living systems without cellular toxicity. A number of chemical ligation strategies have ... "Protein Modification by Strain-Promoted Alkyne-Nitrone Cycloaddition". Angewandte Chemie International Edition. 49 (17): 3065-8 ...
https://en.wikipedia.org/wiki/Bioorthogonal_chemistry
Cdc14Cdc14
Additional data in support of this theory was later obtained from studies in zebrafish, where its Cdc14 proteins were also ... "The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase". EMBO J. 22 (14): 3524-3535. doi: ... Also, while the protein regulates the Cdk1 ortholog of S. pombe, this occurs through a process unlike that of S. cerevisiae; it ... Cdc14 and Cdc14 are a gene and its protein product respectively. Cdc14 is found in most of the eukaryotes. Cdc14 was defined by ...
https://en.wikipedia.org/wiki/Cdc14
BLCAPBLCAP
The protein appears to be down-regulated during bladder cancer progression. The protein also known as BC10 is an 87-amino-acid- ... Although database searches revealed no homology to any human gene at the time of identification, mouse, rat and zebrafish ... Bladder cancer-associated protein is a protein that in humans is encoded by the BLCAP gene. BLCAP was identified using a ... The structure of the protein is predicted to be a globular protein with 2 transmembrane (TM) domains. The human BLCAP gene is ...
https://en.wikipedia.org/wiki/BLCAP
Sphere packing in a cylinderSphere packing in a cylinder
examined the morphologies of virus capsid proteins self-assembled around metal nanorods. Drug particles were coated as densely ... and the notochord of the zebra fish. One of the largest flowers where the berries arrange in a regular cylindrical form is the ...
https://en.wikipedia.org/wiki/Sphere_packing_in_a_cylinder
NEDD9NEDD9
... novel SH2-containing protein family), and other proteins such as the Id family of helix-loop-helix proteins. In terms of post- ... The NEDD9 gene is conserved in Rhesus monkeys, dogs, cows, mice, rats, chickens, zebrafish, and frogs. In vertebrates, it is a ... Neural precursor cell expressed developmentally down-regulated protein 9 (NEDD-9) is a protein that in humans is encoded by the ... NEDD9 is a noncatalytic scaffolding protein that contains docking sites for proteins involved in multiple signal transduction ...
https://en.wikipedia.org/wiki/NEDD9
60S ribosomal protein L7a (zebrafish) | Protein Target - PubChem60S ribosomal protein L7a (zebrafish) | Protein Target - PubChem
... zebrafish). Find diseases associated with this biological target and compounds tested against it in bioassay experiments. ... Protein target information for 60S ribosomal protein L7a ( ...
https://pubchem.ncbi.nlm.nih.gov/protein/A0A2R8Q9K6
Mapping protein phosphorylation in zebrafish developmentMapping protein phosphorylation in zebrafish development
... Reversible protein phosphorylation plays a key role in signaling ... Tyrosine phosphorylation of proteins at two different stages in zebrafish embryonic development was studied by immuno-affinity ... It provides a rapid switch for protein activity as it often changes the conformation and function of a protein in the cell. ... The results established zebrafish as a good model to study in vivo phosphorylation and reveal that surprising homology exists ...
https://dspace.library.uu.nl/handle/1874/26694
Zebrafish Proteins (definition)Zebrafish Proteins (definition)
Leg1b protein, zebrafish 0 *Zebrafish Proteins. PLoS One. 2011;6(8):e22910. Leg1a protein, zebrafish 0 *Zebrafish Proteins. ... Cug2 protein, zebrafish 0 *Nuclear Proteins *Zebrafish Proteins. BMC Dev Biol. 2011;11() (dup#57):49 MSW protein, zebrafish 0 * ... isthmin protein, zebrafish 0 *Zebrafish Proteins. J Cell Mol Med. 2011 Feb;15(2):359-74. TRPV4 protein, zebrafish 0 *Zebrafish ... Ihha protein, zebrafish 0 *Zebrafish Proteins *Hedgehog Proteins. Dev Dyn. 2006 Feb;235(2):478-89. Rbfox2 protein, zebrafish 0 ...
https://reference.md/files/D029/mD029961.html
Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives | - CCMARComparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives | - CCMAR
Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives. ...
https://ccmar.ualg.pt/en/node/1045
Analysis of interferon gamma protein expression in zebrafish (Danio rerio)<...Analysis of interferon gamma protein expression in zebrafish (Danio rerio)<...
Analysis of interferon gamma protein expression in zebrafish (Danio rerio). Sohye Yoon, Ayham Alnabulsi, Steven Bird, Jun Zou, ... It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein ... It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein ... It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein ...
https://abdn.pure.elsevier.com/en/publications/analysis-of-interferon-gamma-protein-expression-in-zebrafish-dani
UO finds bacterial protein that boosts insulin-producing cells in zebrafish | Around the OUO finds bacterial protein that boosts insulin-producing cells in zebrafish | Around the O
A newly discovered bacterial protein produced in the zebrafish gut triggers insulin-producing beta cells of the pancreas to ... Using germ-free zebrafish as a model, lead author and doctoral student Jennifer Hampton Hill explored the possibility that ... Her criteria returned only a single protein candidate that had never been studied. When she made a purified version of it and ... The UO has been a leader in zebrafish research since the 1960s when biologist George Streisinger pioneered a new method for the ...
https://around.uoregon.edu/content/uo-finds-bacterial-protein-boosts-insulin-producing-cells-zebrafish
KOPS.PrPs: Proteins with a purpose ; Lessons from the zebrafishKOPS.PrPs: Proteins with a purpose ; Lessons from the zebrafish
PrPs: Proteins with a purpose ; Lessons from the zebrafish. In: Prion. 3(3), pp. 129-133. ISSN 1933-6896. Available under: doi ... PrPs: Proteins with a purpose ; Lessons from the zebrafish. Type of Publication:. Journal article. ... article{MalagaTrillo2009Prote-19333, title={PrPs: Proteins with a purpose ; Lessons from the zebrafish}, year={2009}, doi={ ... Proteins with a purpose ; Lessons from the zebrafish,/dcterms:title, ,dcterms:bibliographicCitation,First publ. in: Prion ; 3 ( ...
https://kops.uni-konstanz.de/handle/123456789/19333
Elucidating the Role of the Daam Proteins in Zebrafish Embryonic DevelopmentElucidating the Role of the Daam Proteins in Zebrafish Embryonic Development
Together, my results indicate that the zebrafish Daam1a/b and Daam2 behave similarly to Daam1 and Daam2 in frog, respectively, ... Daam1/2 have not been extensively investigated at the genetic level, therefore, I employed the genetic model zebrafish (Danio ... Wnt signaling bifurcates into these two branches at the level of the protein Dishevelled (Dvl). The Dishevelled-associated ... by participating in the non- canonical Wnt signaling pathway and mediating morphology in the developing zebrafish embryo. ...
https://scholarshare.temple.edu/handle/20.500.12613/6545
The Biochemical Analysis of Crystallin Proteins in the Adult Zebrafish Lens | URSCA Proceedings The Biochemical Analysis of Crystallin Proteins in the Adult Zebrafish Lens | URSCA Proceedings
The Biochemical Analysis of Crystallin Proteins in the Adult Zebrafish Lens Authors. * Lauren Piers​and The Kings University ... In order to induce cataracts, 3% hydrogen peroxide was injected into lenses of adult zebrafish and the lens proteins were ... The concentration of the soluble and insoluble proteins was quantified and the lens proteins were further characterized using ... healthy lenses were found to have a higher concentration of soluble proteins and a lower concentration of insoluble proteins. ...
https://journals.macewan.ca/ursca/article/view/961
Gain-of-function mutations in Aqp3a influence zebrafish pigment pattern formation through the tissue environmentGain-of-function mutations in Aqp3a influence zebrafish pigment pattern formation through the tissue environment
The development of the pigmentation pattern in zebrafish is a tightly regulated process that depends on both the self- ... Zebrafish / metabolism* * Zebrafish Proteins / chemistry * Zebrafish Proteins / genetics* * Zebrafish Proteins / metabolism ... Gain-of-function mutations in Aqp3a influence zebrafish pigment pattern formation through the tissue environment Development. ... Here, we describe novel zebrafish mau mutants, which encompass several dominant missense mutations in Aquaporin 3a (Aqp3a) that ...
https://pubmed.ncbi.nlm.nih.gov/28506994/
Tohumluk Creek - WikipediaTohumluk Creek - Wikipedia
"Dietary Protein Requirements of Zebrafish (Dania rerio)". Journal of Limnology and Freshwater Fisheries Research. 5 (1): 34-40 ...
https://en.wikipedia.org/wiki/Tohumluk_Creek
Enhanced incorporation of transgenic DNA into zebrafish chromosomes by a retroviral integration protein - MDC RepositoryEnhanced incorporation of transgenic DNA into zebrafish chromosomes by a retroviral integration protein - MDC Repository
Enhanced incorporation of transgenic DNA into zebrafish chromosomes by a retroviral integration protein ... Enhanced incorporation of transgenic DNA into zebrafish chromosomes by a retroviral integration protein. ...
http://edoc.mdc-berlin.de/5278/
Developmental BiologyDevelopmental Biology
Loss of the RNA-binding protein Rbm15 disrupts liver maturation in zebrafish. Journal of Biological Chemistry ... Wilms tumor protein 1 (WT1) has been implicated in the control of several genes in sexual development, but its function in ... A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori. Journal of ... In this species, Antennapedia (Antp) regulates expression of sericin-1, a major silk protein gene, in the silk gland. Here, we ...
https://www.jbc.org/Developmental-Biology?startPage=&KeywordRaw=gene%20regulation
A systematic genome-wide analysis of zebrafish protein-coding gene function. - MRC Weatherall Institute of Molecular Medicine
So far we have identified potentially disruptive mutations in more than 38% of all known zebrafish protein-coding genes. We ... Here we describe an active project that aims to identify and phenotype the disruptive mutations in every zebrafish protein- ... but again the number of genes studied falls well short of the more than 26,000 zebrafish protein-coding genes. Importantly, for ... this number falls considerably short of the more than 22,000 mouse protein-coding genes. Similarly, in zebrafish genetics, one- ...
https://www.imm.ox.ac.uk/publications/949899
Interphotoreceptor retinoid-binding protein (IRBP) is expressed in the retinal pigment epithelium and pineal gland during...Interphotoreceptor retinoid-binding protein (IRBP) is expressed in the retinal pigment epithelium and pineal gland during...
... is expressed in the retinal pigment epithelium and pineal gland during zebrafish development Academic Article *Overview ...
https://vivo.nkn.uidaho.edu/vivo/display/WA1997WN18601418
Chromosome News, Articles and Research - Page 3Chromosome News, Articles and Research - Page 3
Each chromosome is made up of DNA tightly coiled many times around proteins called histones that support its structure. ... The eyes of mutant zebrafish help understand the role of a protein in preventing cell death Around 15 years ago, a group of ... DNA serves as a template for an intermediary molecule called RNA that, in turn, codes for proteins that control all cellular ... The eyes of these zebrafish did not develop correctly, resulting in them being significantly smaller than the eyes of wild ...
https://www.news-medical.net/condition/Chromosome?page=3
Recently identified protein in zebrafish could be a therapeutic target for resolving birth defects such as cleft palate and...Recently identified protein in zebrafish could be a therapeutic target for resolving birth defects such as cleft palate and...
Home Press Releases Recently identified protein in zebrafish could be a therapeutic target for resolving birth defects such as ... Recently identified protein in zebrafish could be a therapeutic target for resolving birth defects such as cleft palate and ... Le Pabic and his colleagues studied cellular behavior in zebrafish and found that cells of the skeleton do not assemble ... recently identified three proteins, Fat3, Dachsous2 and REREa, which could be targets for resolving birth defects. Le Pabics ...
https://bio.uci.edu/recently-identified-protein-zebrafish-therapeutic-target-resolving-birth-defects-cleft-palate-micrognathia/
Identification of a putative calcium-binding protein as a dioxin-responsive gene in zebrafish and rainbow trout. | College of...
Identification of a putative calcium-binding protein as a dioxin-responsive gene in zebrafish and rainbow trout. ... Identification of a putative calcium-binding protein as a dioxin-responsive gene in zebrafish and rainbow trout.. Title. ... Identification of a putative calcium-binding protein as a dioxin-responsive gene in zebrafish and rainbow trout.. ... Two TCDD-responsive cDNAs, which encode putative calcium-binding proteins, have been isolated from zebrafish and rainbow trout ...
https://appliedecon.oregonstate.edu/biblio/identification-putative-calcium-binding-protein-dioxin-responsive-gene-zebrafish-and-rainbow
Tyrosine hydroxylase-green fluorescence protein transgenic zebrafish as a biosensor and animal model for nicotine and ketamine...
Tyrosine hydroxylase-green fluorescence protein transgenic zebrafish as a biosensor and animal model for nicotine and ketamine ... Tyrosine hydroxylase-green fluorescence protein transgenic zebrafish as a biosensor and animal model for nicotine and ketamine ...
https://scholars.hkbu.edu.hk/en/studentTheses/tyrosine-hydroxylase-green-fluorescence-protein-transgenic-zebraf
Energetic Roles of Hydrogen Bonds at the Ureido Oxygen Binding Pocket in the Streptavidin−Biotin Complex | BiochemistryEnergetic Roles of Hydrogen Bonds at the Ureido Oxygen Binding Pocket in the Streptavidin−Biotin Complex | Biochemistry
An Avidin-Like Protein from Zebrafish. PLoS ONE 2013, 8 (10) , e77207. https://doi.org/10.1371/journal.pone.0077207 ... Avidin related protein 2 shows unique structural and functional features among the avidin protein family. BMC Biotechnology ... DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools. Journal of ... A TAT-streptavidin fusion protein directs uptake of biotinylated cargo into mammalian cells. Protein Engineering, Design and ...
https://pubs.acs.org/doi/10.1021/bi9803123
Chang-Bin Jing, Ph.D. | Harvard Catalyst Profiles | Harvard CatalystChang-Bin Jing, Ph.D. | Harvard Catalyst Profiles | Harvard Catalyst
Zebrafish Proteins. 1. 2019. 1048. 0.110. Why? Sulfonamides. 1. 2021. 1939. 0.110. Why? ...
https://connects.catalyst.harvard.edu/Profiles/display/person/134551/network/researchareas/details
Biblio | Page 37 | Linus Pauling Institute | Oregon State University
Zebrafish Proteins. Beaver LM, Nkrumah-Elie YM, Truong L, Barton CL, Knecht AL, Gonnerman GD, Wong CP, Tanguay RL, Ho E. 2017. ... Zebrafish. McDougall M, Choi J, Truong L, Tanguay R, Traber MG. 2017. Vitamin E deficiency during embryogenesis in zebrafish ... Adverse effects of parental zinc deficiency on metal homeostasis and embryonic development in a zebrafish model.. J Nutr ... Adverse effects of parental zinc deficiency on metal homeostasis and embryonic development in a zebrafish model.. J Nutr ...
https://lpi.oregonstate.edu/biblio?f%5Bauthor%5D=2951&o=asc&page=36&s=keyword
Anti-Strumpellin antibody (ab101222) | AbcamAnti-Strumpellin antibody (ab101222) | Abcam
whole zebrafish protein lysate. Blocking step. Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · ... Lysates/proteins at 10 µg per lane.. Secondary. All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 ... PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated ... Proteins and Peptides. By product type. Proteomics tools. Agonists, activators, antagonists and inhibitors. Cell lines and ...
https://www.abcam.com/strumpellin-antibody-ab101222.html
Whole exome sequencing analysis identifies novel Stargardt disease-related gene mutations in Chinese Stargardt disease and...Whole exome sequencing analysis identifies novel Stargardt disease-related gene mutations in Chinese Stargardt disease and...
... and 8 novel missense variants were conserved across different species and predicted to be damaging to the protein. All 15 novel ... Deletion of the transmembrane protein Prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor ... 3: Multiple sequence alignment of Stargardt-related gene protein across different species.. ... Prominin, a novel microvilli-specific polytopic membrane protein of the apical surface of epithelial cells, is targeted to ...
https://www.nature.com/articles/s41433-021-01525-x?error=cookies_not_supported&code=2189401e-a994-462f-b8a9-f26388706394
MCP: How exercise works its biomolecular wondersMCP: How exercise works its biomolecular wonders
New tools to study protein-protein interactions in zebra fish Nivedita Uday Hegdekar ... Cataloging itty-bitty proteins in large numbers. Dec. 7, 2022. Best of BMB 2022: Ribosome profiling has identified thousands of ... Best of BMB 2022: Proteolysis-targeting chimeras bring together a drug target protein and a ubiquitin ligase to remove the ... Evolutionary constraints on disordered proteins. Dec. 5, 2022. Best of BMB 2022: "Theres evidence that there must be ...
https://www.asbmb.org/asbmb-today/science/120117/mcp-how-exercise-works-its-biomolecular-wonders
Ann Brinkmalm,Ann Brinkmalm,
SILAC zebrafish for quantitative analysis of protein turnover and tissue regeneration. Ann Brinkmalm-Westman, Alexandra ... Zebrafish. Alexandra Abramsson, Ann Brinkmalm-Westman, Josef Pannee, Mikael K Gustavsson, Malin von Otter, Kaj Blennow, Gunnar ... α-Synuclein in Neurodegeneration-A Good Protein that may go Bad. Henrik Zetterberg, Ann Brinkmalm-Westman Brain pathology ( ... A novel pathway for amyloid precursor protein processing. Erik Portelius, Erik Price, Gunnar Brinkmalm, Mark Stiteler, Maria ...
https://www.gu.se/om-universitetet/hitta-person/annbrinkmalm-westman
Facebooks Calibra Is A Secret Weapon for Monetizing Its New Cryptocurrency | Opinion | Communications of the ACMFacebook's Calibra Is A Secret Weapon for Monetizing Its New Cryptocurrency | Opinion | Communications of the ACM
Genomics Study Identifies Unique Set of Proteins That Restores Hearing in Zebrafish National Institutes of Health ...
https://cacm.acm.org/opinion/articles/237577-facebooks-calibra-is-a-secret-weapon-for-monetizing-its-new-cryptocurrency/fulltext
Vol. 105 No. 2 (2020): February, 2020 | Haematologica Vol. 105 No. 2 (2020): February, 2020 | Haematologica
Interferon regulatory factor 2 binding protein 2b regulates neutrophil versus macrophage fate during zebrafish definitive ... Loss of RAF kinase inhibitor protein is involved in myelomonocytic differentiation and aggravates RAS-driven myeloid ... Platelet activation and multidrug resistance protein-4 expression in children and adolescents with different subtypes of ...
https://haematologica.org/issue/view/363
Key Protein for Spinal Cord Repair IdentifiedKey Protein for Spinal Cord Repair Identified
... key protein to repair spinal cord injuries has been discovered by scientists. ... Humans and zebrafish share most protein-coding genes, and CTGF is no exception. The human CTGF protein is nearly 90% similar in ... The effect of the protein is striking," Mokalled said. The second half of the CTGF protein seems to be the key to the healing, ... Its a large protein, made of four smaller parts, and it has more than one function. That might make it easier to deliver and ...
https://www.medindia.net/news/key-protein-for-spinal-cord-repair-identified-164885-1.htm
meis1 regulates cyclin D1 and c-mycexpression, and controls the proliferation of the multipotent cells in the early developing...meis1 regulates cyclin D1 and c-mycexpression, and controls the proliferation of the multipotent cells in the early developing...
Meis family proteins are required for hindbrain development in the zebrafish. Development ... Zebrafish Meis functions to stabilize Pbx proteins and regulate hindbrain patterning. Development ... Of all five zebrafish Meis genes (meis1, 2.1, 2.2, 3 and 4.1), only meis1 and meis2.2 are expressed during early stages of eye ... In addition, Pbx proteins can be retained in the cytoplasm by direct binding to non-muscle myosin II heavy chain B (Huang et al ...
https://journals.biologists.com/dev/article/135/5/799/65019/meis1-regulates-cyclin-D1-and-c-mycexpression-and
EmbryosGenesEmbryonicTransgenicGeneDanio rerio2022MutationsBacterialExtracellularAmino AcidsPeptidesMaturationGeneticsDevelopmentEmbryoTransmembrane proteinsPathwaysMiceSpeciesFluorescentEmbryogenesisToxicityMitochondrialCalcium-bindingReactivityMutantBiologyBovinePrionKinaseSequencePhosphataseMorphogenesisKnockdownExpressionModelAntigenTargetsHumanBindDrugsDefectsPancreasGenomeHEK293Membrane-boundInduceZebraResearchers
Embryos7
  • Morpholino knock down of the kinases Fyn and Yes, induced characteristic phenotypes in 1 day old zebrafish embryos and the lysates induced distinct changes in the PTK activity profiles compared to wild-type lysates. (uu.nl)
  • A quantitative proteomics approach, using stable isotope di-methyl labeling was used to compare protein phosphorylation between wild type and Fyn/Yes knockdown embryos that display convergence and extension cell movement defects. (uu.nl)
  • In this thesis in vivo phosphorylation was studied at different stages and under different experimental conditions in developing zebrafish embryos. (uu.nl)
  • Using knockdown approaches, we previously produced clear PrP loss-of-function phenotypes in zebrafish embryos. (uni-konstanz.de)
  • Le Pabic and his colleagues studied cellular behavior in zebrafish and found that cells of the skeleton do not assemble properly in those embryos with a reduced amount of Fat3-, Dachsous2-, or REREa protein, which resulted in misshapen cartilage structures. (uci.edu)
  • Lipidomics and H2(18)O labeling techniques reveal increased remodeling of DHA-containing membrane phospholipids associated with abnormal locomotor responses in α-tocopherol deficient zebrafish (danio rerio) embryos. (oregonstate.edu)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
Genes18
  • Although gene-targeting approaches and phenotype analysis have led to a detailed understanding of nearly 6,000 protein-coding genes, this number falls considerably short of the more than 22,000 mouse protein-coding genes. (ox.ac.uk)
  • Similarly, in zebrafish genetics, one-by-one gene studies using positional cloning, insertional mutagenesis, antisense morpholino oligonucleotides, targeted re-sequencing, and zinc finger and TAL endonucleases have made substantial contributions to our understanding of the biological activity of vertebrate genes, but again the number of genes studied falls well short of the more than 26,000 zebrafish protein-coding genes. (ox.ac.uk)
  • Importantly, for both mice and zebrafish, none of these strategies are particularly suited to the rapid generation of knockouts in thousands of genes and the assessment of their biological activity. (ox.ac.uk)
  • So far we have identified potentially disruptive mutations in more than 38% of all known zebrafish protein-coding genes. (ox.ac.uk)
  • In addition, 13 novel variants were found in 119 sporadic RP patients in 7 Stargardt disease-related genes, and 8 novel missense variants were conserved across different species and predicted to be damaging to the protein. (nature.com)
  • Of dozens of genes strongly activated by injury, seven coded for proteins that are secreted from cells. (medindia.net)
  • Humans and zebrafish share most protein-coding genes, and CTGF is no exception. (medindia.net)
  • These genes provide instructions for making proteins that are involved in the formation of tissues and organs during embryonic development. (medlineplus.gov)
  • SALL proteins are transcription factors, which means they attach (bind) to specific regions of DNA and help control the activity of particular genes. (medlineplus.gov)
  • Basto-Silva C , Couto A , Rodrigues J , Oliva-Teles A , Navarro I , Kaiya H , Capilla E , Guerreiro I . Feeding frequency and dietary protein/carbohydrate ratio affect feed intake and appetite regulation-related genes expression in gilthead seabream (Sparus aurata). (wjgnet.com)
  • Basto-silva C , Enes P, Oliva-teles A, Balbuena-pecino S, Navarro I, Capilla E, Guerreiro I. Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata). (wjgnet.com)
  • Here, focusing on potential candidate genes for angiogenesis, we performed a morpholino-based genetic screen in zebrafish and identified Cavin-2, a membrane-bound phosphatidylserine-binding protein and critical organizer of caveolae (small microdomains in the plasma membrane), as a regulator of angiogenesis. (a-star.edu.sg)
  • Most mitochondrial proteins are encoded by nuclear genes, synthetized in the cytosol and targeted into the organelle. (upf.edu)
  • Among the ~20,000 protein-coding genes in the human genome, it has been determined that a remarkably small number of conserved signaling systems (Notch, Wnt, Shh, Jak/Stat, RTK, TGF-β, NF-κB) control the vast majority of cell fate decisions. (bioscience.fi)
  • A cascade effect occurs when ras -GTPase is "switched on" by incoming signals, leading to activation of other proteins, which, in turn, activate genes responsible for cell growth and differentiation. (medscape.com)
  • Mutations in ras genes can cause permanent activation of ras proteins. (medscape.com)
  • Group leader Professor Dr. Rudolf Wiesner added, "Mutations in the genes that code for these proteins lead to severe neurological diseases, in VPS35 for example Parkinson's disease. (phys.org)
  • Persons with congenital muscular dystrophies due to mutations in genes for selenoprotein N and in genes for the extracellular matrix proteins integrin-α7 and collagen type VI have normal brain MRI findings. (medscape.com)
Embryonic4
  • Tyrosine phosphorylation of proteins at two different stages in zebrafish embryonic development was studied by immuno-affinity purification and multi-dimensional LC-MS. Tyrosine kinases including members of the Src family of kinases, Eph receptors and Focal adhesion kinase as well as adaptor proteins such as Paxillin, Crk and p130Cas were identified. (uu.nl)
  • Using techniques such as the latest gene-editing system CRISPR/Cas9 and other well-established molecular methods including in situ hybridization, RT-PCR and knockdown using morpholino oligonucleotides, I sought to further establish the role of the Daam protein family in vertebrate embryonic development. (temple.edu)
  • Adverse effects of parental zinc deficiency on metal homeostasis and embryonic development in a zebrafish model. (oregonstate.edu)
  • High-throughput technology has increased the utility of zebrafish embryonic toxicity assays in screening of chemicals and drugs for toxicity or effect. (cdc.gov)
Transgenic2
  • Functional studies showed that ectopic expression of bovine TWIST2 in neural crest in transgenic zebrafish led to a decrease in melanocyte numbers. (plos.org)
  • 16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)
Gene17
  • A systematic genome-wide analysis of zebrafish protein-coding gene function. (ox.ac.uk)
  • Here we describe an active project that aims to identify and phenotype the disruptive mutations in every zebrafish protein-coding gene, using a well-annotated zebrafish reference genome sequence, high-throughput sequencing and efficient chemical mutagenesis. (ox.ac.uk)
  • Identification of a putative calcium-binding protein as a dioxin-responsive gene in zebrafish and rainbow trout. (oregonstate.edu)
  • Taken together, these findings demonstrate in zebrafish and rainbow trout that dioxin increases expression of this EF-hand calcium-binding protein gene in a tissue-dependent fashion. (oregonstate.edu)
  • Fig. 3: Multiple sequence alignment of Stargardt-related gene protein across different species. (nature.com)
  • Here we investigate whether members of the Meis gene family, the vertebrate homologs of hth , are also involved in early stages of eye development in the zebrafish. (biologists.com)
  • Most SALL4 gene mutations create a premature stop signal in the instructions for making the SALL4 protein. (medlineplus.gov)
  • As a result, cells do not produce any functional protein from one copy of this gene. (medlineplus.gov)
  • The SALL4 gene mutation responsible for IVIC syndrome creates a premature stop signal in the instructions for making the SALL4 protein. (medlineplus.gov)
  • As a result, cells produce an abnormally short version of the protein from one copy of this gene. (medlineplus.gov)
  • Mutations to the SPG4 gene encoding the microtubule-severing protein spastin are the most common cause of hereditary spastic paraplegia. (jneurosci.org)
  • Use of rice protein concentrates in Oreochromis niloticus diets and its effect on growth, intestinal morphology, biochemical indices and ghrelin gene expression. (wjgnet.com)
  • They all share a newly-identified homozygous genetic variant in the gene CLCC1 , encoding a putative protein channel present inside all cells. (plos.org)
  • Our studies demonstrate that alterations to this gene and protein channel specifically affect cell survival and eye development in cell culture, as well as retinal structure and physiology in genetically-altered zebrafish and mice. (plos.org)
  • The NF1 gene product is a cytoplasmic protein called neurofibromin 1, which appears to have diverse functions in many different tissues. (medscape.com)
  • 5) The primers for the Histone 3 gene, one of the most highly conserved proteins, gave variable results and amplified DNA from V fischeri which does not contain histone proteins. (suny.edu)
  • Role of the planar cell polarity gene Protein tyrosine kinase 7 in neural tube defects in humans. (cdc.gov)
Danio rerio6
  • Daam1/2 have not been extensively investigated at the genetic level, therefore, I employed the genetic model zebrafish (Danio rerio) to further clarify their role in Wnt signaling. (temple.edu)
  • Zebrafish (Danio rerio) have become important model organisms to increase human understanding of ophthalmological diseases, specifically cataracts. (macewan.ca)
  • 2022. Non-invasive sampling of water-borne hormones demonstrates individual consistency of the cortisol response to stress in laboratory zebrafish (Danio rerio). (awionline.org)
  • 2022. FELASA-AALAS recommendations for monitoring and reporting of laboratory fish diseases and health status, with an emphasis on zebrafish (Danio rerio). (awionline.org)
  • The laboratory zebrafish (Danio rerio) is now an accepted model in toxicologic research. (cdc.gov)
  • Dietary lysine imbalance affects muscle proteome in zebrafish (Danio rerio): a comparative 2D-DIGE study. (ualg.pt)
20222
  • Best of BMB 2022: Proteolysis-targeting chimeras bring together a drug target protein and a ubiquitin ligase to remove the target from the cell. (asbmb.org)
  • Ayesha Sen et al, Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA, Nature Communications (2022). (phys.org)
Mutations5
  • Here, we describe novel zebrafish mau mutants, which encompass several dominant missense mutations in Aquaporin 3a (Aqp3a) that lead to broken stripes and short fins. (nih.gov)
  • Here, we used a screen for mutations affecting liver development in zebrafish and identified a cq96 mutant that exhibits a specific defect in liver maturation. (jbc.org)
  • Researchers from the National Institutes of Health have developed a three-dimensional structure that allows them to see how and where disease mutations on the twinkle protein can lead to mitochondrial diseases. (news-medical.net)
  • A team of researchers from the University of Cologne's Physiology Center at the Faculty of Medicine, the Center for Molecular Medicine Cologne (CMMC) and the CECAD Cluster of Excellence for Aging Research has now shown that mutations of the mtDNA lead to a local rearrangement of proteins in the mitochondrial membrane. (phys.org)
  • Transcript is thought to undergo nonsense mediated decay, a process which detects nonsense mutations and prevents the expression of truncated or erroneous proteins. (ensembl.org)
Bacterial3
  • A newly discovered bacterial protein produced in the zebrafish gut triggers insulin-producing beta cells of the pancreas to multiply during early larval development, say University of Oregon researchers. (uoregon.edu)
  • This restoration became the basis for her search and ultimate discovery of a novel bacterial protein that on its own could stimulate the growth of insulin-producing cells. (uoregon.edu)
  • Mature GH protein was efficiently expressed in bacterial and zebrafish systems using appropriate expression vectors. (who.int)
Extracellular2
  • In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal-regulated kinase (MEK)/extracellular signal-regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)
  • Laminins are extracellular glycoproteins that bind with other extracellular and transmembrane proteins to form the frame of the basal lamina that surrounds individual myofibers. (medscape.com)
Amino Acids2
  • Dietary indispensable amino acids profile affects protein utilization and growth of Senegalese sole larvae. (ualg.pt)
  • Recombinant full length protein, corresponding to amino acids 1-673 of Human Annexin VI with an N terminal His tag. (creativebiomart.net)
Peptides2
  • An on-line TiO2 based LC MS/MS was used to enrich for all phosphorylated peptides in 1 day old zebrafish embryo samples. (uu.nl)
  • To understand how some of these effects are controlled on a cellular level, researchers at the University of Sydney did a proteomic analysis of the swarm of peptides and small-protein hormones that circulate throughout the body in blood plasma. (asbmb.org)
Maturation3
  • Her research involves exploring B cell development and maturation in zebrafish, and she recently had her findings published in the medical journal Blood . (pointloma.edu)
  • My students and I are exploring B cell development and maturation in zebrafish. (pointloma.edu)
  • The protein VPS35, the main component of the retromer, mediates the maturation of early endosomes into late autophagy vesicles, where degradation and recycling ultimately take place," said Pla-Martin. (phys.org)
Genetics1
  • The UO has been a leader in zebrafish research since the 1960s when biologist George Streisinger pioneered a new method for the study of vertebrate development and genetics with the introduction of the small fish as a model organism. (uoregon.edu)
Development17
  • Mapping protein phosphorylation in zebrafish development Reversible protein phosphorylation plays a key role in signaling processes that are vital for a cell and organism. (uu.nl)
  • Therefore we have explored the possibilities to use zebrafish development as a model system to study in vivo phosphorylation using mass spectrometry. (uu.nl)
  • Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY). (reference.md)
  • Using germ-free zebrafish as a model, lead author and doctoral student Jennifer Hampton Hill explored the possibility that certain gut bacteria are necessary for the pancreas to populate itself with a robust number of beta cells during development. (uoregon.edu)
  • The finding that specific gut bacteria produce proteins that stimulate beta cell development sheds new light on epidemiological data connecting low-diversity childhood microbiomes with increased Type 1 diabetes risk. (uoregon.edu)
  • The development of the pigmentation pattern in zebrafish is a tightly regulated process that depends on both the self-organizing properties of pigment cells and extrinsic cues from other tissues. (nih.gov)
  • It has been found that there are conserved visual pathways between zebrafish and humans, which allow the study of cataracts in zebrafish to be exceptionally insightful for the mechanisms behind cataract development in humans. (macewan.ca)
  • This protein may also be important for the development of nerves that control eye movement and for the formation of the walls (septa) that divide the heart into separate chambers. (medlineplus.gov)
  • Researchers are investigating how a reduction in the amount of the SALL4 protein disrupts eye, heart, and limb development in people with Duane-radial ray syndrome and acro-renal-ocular syndrome. (medlineplus.gov)
  • However, the relatively slow and sporadic development of kidney cysts in polycystin 1 and cilia protein conditional knockout models has suggested that other factors may contribute to cyst formation. (asnjournals.org)
  • Almost 200 students from nine high schools visited MIT on March 24 and 25 to learn about protein structure and folding and zebrafish development. (mit.edu)
  • We hypothesize that cholesterol dysfunction may lead to ASD by three mechanisms working in concert during brain development: (1) impaired sonic hedgehog patterning, (2) alterations in membrane lipid raft structure and protein function resulting in abnormal synaptic plasticity, and (3) impaired neurosteroid synthesis. (hindawi.com)
  • Previous studies of CFC1 (CRYPTIC), another member of the EGF-CFC family, demonstrated that normal function of this protein is required for proper laterality development in humans. (princeton.edu)
  • The mutant protein is inactive in a zebrafish rescue assay, indicating a role for TDGF1 in human midline and forebrain development. (princeton.edu)
  • The atomic-level structure of SOCS1 binding to its partner protein JAK could guide the development of drugs that alter disease-causing cell signalling pathways, and may have applications for treating some blood cancers, including leukaemias . (edu.au)
  • Above: The structure of SOCS1 (red) bound to a JAK protein (beige) could inform the development of new drugs to treat cancer. (edu.au)
  • The Protein kinase C (PKC) -associated sign pathway performs essential roles in regulation of cell development, differentiation and apoptosis. (aabioetica.org)
Embryo2
  • The in vitro kinase chip technology was also used to determine protein tyrosine kinase (PTK) activity profiles from morpholino mediated knock down zebrafish embryo lysates. (uu.nl)
  • Together, my results indicate that the zebrafish Daam1a/b and Daam2 behave similarly to Daam1 and Daam2 in frog, respectively, by participating in the non- canonical Wnt signaling pathway and mediating morphology in the developing zebrafish embryo. (temple.edu)
Transmembrane proteins1
  • Polycystin 1 and polycystin 2 are multipass transmembrane proteins that function together as a receptor-ion channel complex, 1 leading to the widely held view that primary cellular defects in polycystin-deficient cells are caused by a disruption of normal Ca 2+ signaling. (asnjournals.org)
Pathways2
  • As a consequence protein phosphorylation and the elucidation of signaling pathways have received considerable attention over the past years. (uu.nl)
  • Explore pathways + proteins related to this product. (cellsignal.com)
Mice3
  • Based on the functions of similar proteins in other organisms (such as zebrafish and mice), the SALL4 protein appears to play a critical role in the developing limbs. (medlineplus.gov)
  • This groundbreaking research by scientists at the University of Virginia has demonstrated a novel way to control the nervous systems of zebrafish and mice using magnets. (thechattymammoth.com)
  • d, e Western blot analysis and quantification of the indicated proteins in muscle extracts from these mice. (phys.org)
Species2
  • It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein expression and bioactivity in fish. (elsevier.com)
  • Zebrafish, like all fish species, use sound to learn about their environment. (awionline.org)
Fluorescent5
  • In collaboration with scientists from UCSD and the University of Alberta, Canada, we have marked B cell populations with fluorescent proteins to understand how and where the B cells develop in zebrafish and how and where they respond to pathogens. (pointloma.edu)
  • Future directions in zebrafish research are predicted to take advantage of CRISPR-Cas9 genome editing methods in creating models of disease and interrogating mechanisms of action with fluorescent reporters or tagged proteins. (cdc.gov)
  • Using a fluorescent protein, mCherry, active remyelination and demyelination can actually be visualized glowing in the living zebrafish. (mslivingwell.org)
  • Optical consequences of a genetically-encoded voltage indicator with a pH sensitive fluorescent protein. (neurotree.org)
  • A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice. (neurotree.org)
Embryogenesis1
  • Vitamin E deficiency during embryogenesis in zebrafish causes lasting metabolic and cognitive impairments despite refeeding adequate diets. (oregonstate.edu)
Toxicity3
  • However, demonstration that the encoded S100A4 proteins actually bind calcium and play a role in dioxin toxicity will require further study. (oregonstate.edu)
  • Our results fortify a model wherein toxicity of mutant spastin proteins, especially mutant M1, contributes to axonal degeneration in the corticospinal tracts. (jneurosci.org)
  • Zebrafish can also model developmental origins of health and disease and multi- and transgenerational toxicity. (cdc.gov)
Mitochondrial2
  • The results have appeared in Nature Communications under the title "Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA. (phys.org)
  • Their removal is coordinated by the interaction of the nucleoid protein Twinkle and the mitochondrial membrane proteins SAMM50 and ATAD3. (phys.org)
Calcium-binding2
  • Two TCDD-responsive cDNAs, which encode putative calcium-binding proteins, have been isolated from zebrafish and rainbow trout. (oregonstate.edu)
  • altered ER calcium handling in ADPKD and the involvement of multiple calcium binding proteins in vesicular trafficking suggest that ADPKD could affect secretion. (asnjournals.org)
Reactivity1
  • Primary antibodies directed to mammalian target proteins have been characterized for reactivity against mouse, rat and human proteins. (scbt.com)
Mutant3
  • Around 15 years ago, a group of researchers discovered mutant zebrafish. (news-medical.net)
  • Indeed, early studies of ADPKD patient cells revealed defects in ECM proteoglycan synthesis in ADPKD cells, 10 - 13 whereas more recently, Golgi function and basolateral exocytosis have been shown to be altered in PKD1 mutant epithelial cells, 14 with altered intracellular vesicle trafficking linked to mislocalization of sec6, sec8, and E-cadherin proteins. (asnjournals.org)
  • The p.D25E alteration decreased CLCC1 channel function accompanied by accumulation of mutant protein in granules within the ER lumen, while siRNA knockdown of CLCC1 mRNA induced apoptosis in cultured ARPE-19 cells. (plos.org)
Biology5
  • Here we discuss the use of the zebrafish in prion biology and how it may advance our understanding of the roles of PrP in health and disease. (uni-konstanz.de)
  • Pierre Le Pabic, postdoctoral fellow in Developmental and Cell Biology in the laboratory of Professor Thomas Schilling, recently identified three proteins, Fat3, Dachsous2 and REREa, which could be targets for resolving birth defects. (uci.edu)
  • Structural biology researchers (from left) Dr Nick Liau, Associate Professor Jeff Babon and Dr Nadia Kershaw have revealed how SOCS1 proteins 'switch off' cell signalling. (edu.au)
  • Our researchers have used structural biology to visualise how SOCS1 binds to JAK proteins in never-before seen detail. (edu.au)
  • My group integrates approaches in structural biology, cellular biology, and protein engineering to study developmental receptor signaling. (bioscience.fi)
Bovine2
  • channel of the bovine rumen: localization and functional characterization of a protein relevant for ruminal ammonia transport. (xenbase.org)
  • Many of our mammalian antibodies are reactive with equine, bovine, canine, feline, caprine, porcine and ovine protein targets and are suitable for veterinary research. (scbt.com)
Prion1
  • The best-known attribute of the prion protein (PrP) is its tendency to misfold into a rogue isoform. (uni-konstanz.de)
Kinase3
  • Protein phosphatase 2A holoenzymes regulate leucine-rich repeat kinase 2 phosphorylation and accumulation. (xenbase.org)
  • Differentiation-Associated Expression of Conventional Protein Kinase C Isoforms in Primary Cultures of Bone Marrow Cells Induced by M-CSF and G-CSF. (aabioetica.org)
  • The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). (cellsignal.com)
Sequence1
  • Castellini M , Spagnolli G, Biasini E, Casarosa S, Messina A. Expression and protein sequence analyses of zebrafish impg2a and impg2b , two proteoglycans of the interphotoreceptor matrix. (wjgnet.com)
Phosphatase3
  • Predicted to enable protein serine/threonine phosphatase activity. (nih.gov)
  • Orthologous to human PHLPP2 (PH domain and leucine rich repeat protein phosphatase 2). (nih.gov)
  • Endothelial Scaffolding Protein ENH (Enigma Homolog Protein) Promotes PHLPP2 (Pleckstrin Homology Domain and Leucine-Rich Repeat Protein Phosphatase 2)-Mediated Dephosphorylation of AKT1 and eNOS (Endothelial NO Synthase) Promoting Vascular Remodeling. (nih.gov)
Morphogenesis2
  • The Dishevelled-associated activator of morphogenesis 1 (Daam1) protein was identified via a yeast-two hybrid screen using Dvl as bait. (temple.edu)
  • Focal adhesions are essential to drive zebrafish heart valve morphogenesis. (mpg.de)
Knockdown1
  • In zebrafish, polycystin 2 knockdown induces kidney cysts, hydrocephalus, left/right asymmetry defects, and strong dorsal axis curvature. (asnjournals.org)
Expression5
  • Next we analysed the production of the native protein following expression induced by PHA stimulation of leukocytes in vitro or antigen re-stimulation in vivo. (elsevier.com)
  • We show the IFN-γ protein is produced as a dimer, and that a good correlation exists between transcript expression levels and protein levels. (elsevier.com)
  • Increased collagen II protein accumulation did not associate with increased col2a1 mRNA or a decrease in matrix metalloproteinase activity but, instead, it associated with increased expression of the endoplasmic reticulum/Golgi transport coat protein complex II Sec proteins. (asnjournals.org)
  • The depressed electroretinogram (ERG) cone response and cone spectral sensitivity of 5 dpf KO zebrafish and reduced eye size, retinal thickness, and expression of rod and cone opsins could be rescued by injection of wild type CLCC1 mRNA. (plos.org)
  • All SignalSilence ® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. (cellsignal.com)
Model5
  • The results established zebrafish as a good model to study in vivo phosphorylation and reveal that surprising homology exists with human phosphoproteins. (uu.nl)
  • Using a zebrafish model, we report that Papp-aa regulates bone calcification by promoting Ca 2+ -transporting epithelial cell (ionocyte) quiescence-proliferation transition. (elifesciences.org)
  • Evaluation of the roles imposed by HMGB1 protein in MPTP-induced Parkinson Disease Zebra fish model. (iium.edu.my)
  • The zebrafish model fills a niche between in vitro models and mammalian biomedical models. (cdc.gov)
  • The zebrafish has many advantages as a toxicologic model and new methodologies and areas of study continue to expand the usefulness and application of the zebrafish. (cdc.gov)
Antigen2
  • Particle-based transcutaneous administration of HIV-1 p24 protein to human skin explants and targeting of epidermal antigen presenting cells. (adjuvatis.com)
  • Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). (novusbio.com)
Targets3
  • We offer monoclonal antibodies directed against a broad range of mammalian and non-mammalian protein targets, representing essentially all targets covered by polyclonal antibodies. (scbt.com)
  • We now want to use these proteins as new molecular targets to open up entirely new treatment options for these kinds of aging-associated diseases. (phys.org)
  • and their connection to the mitochondria (Oncogene 2007, 26(54):7569-7575), proposing that some of these proteins could be therapeutic targets. (cancer-functional-genomics.org)
Human3
  • When Hampton Hill purified two of these related proteins from human bacteria, they proved to be equally potent at stimulating beta cell expansion in zebrafish. (uoregon.edu)
  • The zebrafish and rainbow trout sequences are 88% similar to each other at the amino acid level and are orthologs of the human S100A4 calcium-binding protein. (oregonstate.edu)
  • The human CTGF protein is nearly 90% similar in its amino acid building blocks to the zebrafish form. (medindia.net)
Bind1
  • Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
Drugs1
  • He also was one of the first researchers that described the role of Inhibitor Apoptosis Proteins (IAPs) on anti-apoptotic signaling cascades that control the response to chemotherapeutic drugs (Biochem Pharmacol 2001, 62(1): 13-19, Cancer Lett 2012, 318(1):61-67. (cancer-functional-genomics.org)
Defects2
  • Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport. (nature.com)
  • VANGL1 rare variants associated with neural tube defects affect convergent extension in zebrafish. (cdc.gov)
Pancreas1
  • For example, we have applied light-activatable cMOs to interrogate transcription factor function during zebrafish notochord, pancreas, and vascular patterning. (cdc.gov)
Genome1
  • She next turned to the genome sequences of the previously screened bacteria and asked which of the prospective proteins were shared by the beta cell-expanding bacteria and absent from inert bacteria. (uoregon.edu)
HEK2931
  • We show that the zfIFN-γ moAb specifically recognises E. coli produced recombinant IFN-γ protein and zfIFN-γ produced in transfected HEK293 cells, by Western blot analysis. (elsevier.com)
Membrane-bound1
  • Parker and colleagues are continuing their proteomic analyses by examining the functional surfaces of exosomes, which are small membrane-bound vesicles used to send proteins, DNA and RNA between distant cells and organs, and they hope to publish their follow-up results soon. (asbmb.org)
Induce2
  • In order to induce cataracts, 3% hydrogen peroxide was injected into lenses of adult zebrafish and the lens proteins were separated into their soluble and insoluble protein fractions through centrifugation. (macewan.ca)
  • Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis. (bvsalud.org)
Zebra1
  • Scientists have been studying the lens of the eye of zebra fish and specifically proteins called alpha crystallins. (fbresearch.org)
Researchers2
  • Walter and Eliza Hall Institute researchers have visualised for the first time how the protein SOCS1 'switches off' cell signalling to dampen immune responses and block cancer growth. (edu.au)
  • Researchers in Korea have worked on genetically-engineered zebrafish to develop a way to study remyelination for multiple sclerosis. (mslivingwell.org)
Chromosome News, Articles and Research - Page 3
Chromosome News, Articles and Research - Page 3 (news-medical.net)
Chang-Bin Jing, Ph.D. | Harvard Catalyst Profiles | Harvard Catalyst
Chang-Bin Jing, Ph.D. | Harvard Catalyst Profiles | Harvard Catalyst (connects.catalyst.harvard.edu)
Anti-Strumpellin antibody (ab101222) | Abcam
Anti-Strumpellin antibody (ab101222) | Abcam (abcam.com)
Whole exome sequencing analysis identifies novel Stargardt disease-related gene mutations in Chinese Stargardt disease and...
Whole exome sequencing analysis identifies novel Stargardt disease-related gene mutations in Chinese Stargardt disease and... (nature.com)
Germline stem cells in human | Signal Transduction and Targeted Therapy
Germline stem cells in human | Signal Transduction and Targeted Therapy (nature.com)
Facebook's Calibra Is A Secret Weapon for Monetizing Its New Cryptocurrency | Opinion | Communications of the ACM
Facebook's Calibra Is A Secret Weapon for Monetizing Its New Cryptocurrency | Opinion | Communications of the ACM (cacm.acm.org)
The Rise of Serverless Computing | December 2019 | Communications of the ACM
The Rise of Serverless Computing | December 2019 | Communications of the ACM (cacm.acm.org)
Analysis of interferon gamma protein expression in zebrafish (Danio rerio)<...
Analysis of interferon gamma protein expression in zebrafish (Danio rerio)<... (abdn.pure.elsevier.com)
Key Protein for Spinal Cord Repair Identified
Key Protein for Spinal Cord Repair Identified (medindia.net)
Kctd13 deletion reduces synaptic transmission via increased RhoA | Nature
Kctd13 deletion reduces synaptic transmission via increased RhoA | Nature (nature.com)
SALL4 gene: MedlinePlus Genetics
SALL4 gene: MedlinePlus Genetics (medlineplus.gov)
Meis1 regulates cyclin D1 and c-mycexpression, and controls the proliferation of the multipotent cells in the early developing...
Meis1 regulates cyclin D1 and c-mycexpression, and controls the proliferation of the multipotent cells in the early developing... (journals.biologists.com)
Vesa Olkkonen - Publications - University of Helsinki
Vesa Olkkonen - Publications - University of Helsinki (researchportal.helsinki.fi)
UO finds bacterial protein that boosts insulin-producing cells in zebrafish | Around the O
UO finds bacterial protein that boosts insulin-producing cells in zebrafish | Around the O (around.uoregon.edu)
Deficiency of the SMOC2 matricellular protein impairs bone healing and produces age-dependent bone loss | Scientific Reports
Deficiency of the SMOC2 matricellular protein impairs bone healing and produces age-dependent bone loss | Scientific Reports (nature.com)
Permalien vers IFN-Stimulated Genes in Zebrafish and Humans Define an Ancient Arsenal of Antiviral Immunity.
Permalien vers IFN-Stimulated Genes in Zebrafish and Humans Define an Ancient Arsenal of Antiviral Immunity. (research.pasteur.fr)
Loss of the multifunctional RNA-binding protein RBM47 as a source of selectable metastatic traits in breast cancer | eLife
Loss of the multifunctional RNA-binding protein RBM47 as a source of selectable metastatic traits in breast cancer | eLife (elifesciences.org)
Effective targeted gene 'knockdown' in zebrafish | Nature Genetics
Effective targeted gene 'knockdown' in zebrafish | Nature Genetics (nature.com)
KOPS.PrPs: Proteins with a purpose ; Lessons from the zebrafish
KOPS.PrPs: Proteins with a purpose ; Lessons from the zebrafish (kops.uni-konstanz.de)
Targeted protein degradation as a powerful research tool in basic biology and drug target discovery | Nature Structural &...
Targeted protein degradation as a powerful research tool in basic biology and drug target discovery | Nature Structural &... (nature.com)
Vision - Foundation for Biomedical Research
Vision - Foundation for Biomedical Research (fbresearch.org)
Xenopus Anatomy Ontology: Papers for
Xenopus Anatomy Ontology: Papers for (xenbase.org)
Plus it
Plus it (jneurosci.org)
BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis | Development | The Company of...
BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis | Development | The Company of... (journals.biologists.com)