An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).
The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.
ANIMALS whose GENOME has been altered by GENETIC ENGINEERING, or their offspring.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Synthetic analogs of NUCLEIC ACIDS composed of morpholine ring derivatives (MORPHOLINES) linked by phosphorodimidates. One standard DNA nucleic acid base (ADENINE; GUANINE; CYTOSINE; OR THYMINE) is bound to each morpholine ring.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
The processes occurring in early development that direct morphogenesis. They specify the body plan ensuring that cells will proceed to differentiate, grow, and diversify in size and shape at the correct relative positions. Included are axial patterning, segmentation, compartment specification, limb position, organ boundary patterning, blood vessel patterning, etc.
Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.
Membranous appendage of fish and other aquatic organisms used for locomotion or balance.
Aquatic vertebrate sensory system in fish and amphibians. It is composed of sense organs (canal organs and pit organs) containing neuromasts (MECHANORECEPTORS) that detect water displacement caused by moving objects.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The posterior of the three primitive cerebral vesicles of an embryonic brain. It consists of myelencephalon, metencephalon, and isthmus rhombencephali from which develop the major BRAIN STEM components, such as MEDULLA OBLONGATA from the myelencephalon, CEREBELLUM and PONS from the metencephalon, with the expanded cavity forming the FOURTH VENTRICLE.
Paired, segmented masses of MESENCHYME located on either side of the developing spinal cord (neural tube). Somites derive from PARAXIAL MESODERM and continue to increase in number during ORGANOGENESIS. Somites give rise to SKELETON (sclerotome); MUSCLES (myotome); and DERMIS (dermatome).
Morphological and physiological development of EMBRYOS.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
Members of the transforming growth factor superfamily that play a role in pattern formation and differentiation during the pregastrulation and GASTRULATION stages of chordate development. Several nodal signaling ligands are specifically involved in the genesis of left-right asymmetry during development. The protein group is named after a critical region of the vertebrate embryo PRIMITIVE STREAK referred to as HENSEN'S NODE.
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
A process of complicated morphogenetic cell movements that reorganizes a bilayer embryo into one with three GERM LAYERS and specific orientation (dorsal/ventral; anterior/posterior). Gastrulation describes the germ layer development of a non-mammalian BLASTULA or that of a mammalian BLASTOCYST.
A cartilaginous rod of mesodermal cells at the dorsal midline of all CHORDATE embryos. In lower vertebrates, notochord is the backbone of support. In the higher vertebrates, notochord is a transient structure, and segments of the vertebral column will develop around it. Notochord is also a source of midline signals that pattern surrounding tissues including the NEURAL TUBE development.
The relationships of groups of organisms as reflected by their genetic makeup.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A region, of SOMITE development period, that contains a number of paired arches, each with a mesodermal core lined by ectoderm and endoderm on the two sides. In lower aquatic vertebrates, branchial arches develop into GILLS. In higher vertebrates, the arches forms outpouchings and develop into structures of the head and neck. Separating the arches are the branchial clefts or grooves.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Formation of differentiated cells and complicated tissue organization to provide specialized functions.
An early non-mammalian embryo that follows the MORULA stage. A blastula resembles a hollow ball with the layer of cells surrounding a fluid-filled cavity (blastocele). The layer of cells is called BLASTODERM.
Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.
The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Chromatophores (large pigment cells of fish, amphibia, reptiles and many invertebrates) which contain melanin. Short term color changes are brought about by an active redistribution of the melanophores pigment containing organelles (MELANOSOMES). Mammals do not have melanophores; however they have retained smaller pigment cells known as MELANOCYTES.
A family of intercellular signaling proteins that play and important role in regulating the development of many TISSUES and organs. Their name derives from the observation of a hedgehog-like appearance in DROSOPHILA embryos with genetic mutations that block their action.
A genus of pufferfish commonly used for research.
The paired caudal parts of the PROSENCEPHALON from which the THALAMUS; HYPOTHALAMUS; EPITHALAMUS; and SUBTHALAMUS are derived.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The primordial kidney that develops from the INTERMEDIATE MESODERM in the embryos of vertebrates, and is succeeded by the MESONEPHROS. In higher vertebrates and humans, the pronephros is a vestigial and transient structure.
The two longitudinal ridges along the PRIMITIVE STREAK appearing near the end of GASTRULATION during development of nervous system (NEURULATION). The ridges are formed by folding of NEURAL PLATE. Between the ridges is a neural groove which deepens as the fold become elevated. When the folds meet at midline, the groove becomes a closed tube, the NEURAL TUBE.
The physiological renewal, repair, or replacement of tissue.
The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.
The only genus in the family Oryziinae, order BELONIFORMES. Oryzias are egg-layers; other fish of the same order are livebearers. Oryzias are used extensively in testing carcinogens.
A large family of structurally-related transcription factors that were originally discovered based upon their close sequence homology to an HMG-box domain found in SEX-DETERMINING REGION Y PROTEIN. Many SOX transcription factors play important roles in regulating CELL DIFFERENTIATION. The numerous members of this family are organized in several subgroups according to structural identities found within the proteins.
An activity in which the body is propelled through water by specific movement of the arms and/or the legs. Swimming as propulsion through water by the movement of limbs, tail, or fins of animals is often studied as a form of PHYSICAL EXERTION or endurance.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes.
The entire nerve apparatus, composed of a central part, the brain and spinal cord, and a peripheral part, the cranial and spinal nerves, autonomic ganglia, and plexuses. (Stedman, 26th ed)
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Thin-walled sacs or spaces which function as a part of the respiratory system in birds, fishes, insects, and mammals.
Short fragments of DNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
Used in the form of its salts as a dye and as an intermediate in manufacture of Acid Yellow, diazo dyes, and indulines.
A paired box transcription factor that is essential for ORGANOGENESIS of the CENTRAL NERVOUS SYSTEM and KIDNEY.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Proteins containing a region of conserved sequence, about 200 amino acids long, which encodes a particular sequence specific DNA binding domain (the T-box domain). These proteins are transcription factors that control developmental pathways. The prototype of this family is the mouse Brachyury (or T) gene product.
A family of conserved cell surface receptors that contain EPIDERMAL GROWTH FACTOR repeats in their extracellular domain and ANKYRIN repeats in their cytoplasmic domains. The cytoplasmic domain of notch receptors is released upon ligand binding and translocates to the CELL NUCLEUS where it acts as transcription factor.
The inner of the three germ layers of an embryo.
Animals having a vertebral column, members of the phylum Chordata, subphylum Craniata comprising mammals, birds, reptiles, amphibians, and fishes.
The organ of sight constituting a pair of globular organs made up of a three-layered roughly spherical structure specialized for receiving and responding to light.
A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.
Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
A fibroblast growth factor that is expressed primarily during development.
Proteins obtained from species of fish (FISHES).
The essential part of the hearing organ consists of two labyrinthine compartments: the bony labyrinthine and the membranous labyrinth. The bony labyrinth is a complex of three interconnecting cavities or spaces (COCHLEA; VESTIBULAR LABYRINTH; and SEMICIRCULAR CANALS) in the TEMPORAL BONE. Within the bony labyrinth lies the membranous labyrinth which is a complex of sacs and tubules (COCHLEAR DUCT; SACCULE AND UTRICLE; and SEMICIRCULAR DUCTS) forming a continuous space enclosed by EPITHELIUM and connective tissue. These spaces are filled with LABYRINTHINE FLUIDS of various compositions.
A small order of primarily marine fish containing 340 species. Most have a rotund or box-like shape. TETRODOTOXIN is found in their liver and ovaries.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Alkaloids with powerful hypotensive effects isolated from American or European Hellebore (Veratrum viride Ait. Liliaceae and Veratrum album L. Liliaceae). They increase cholinergic and decrease adrenergic tone with appropriate side effects and at higher doses depress respiration and produce cardiac arrhythmias; only the ester alkaloids have been used as hypotensive agents in specific instances. They have been generally replaced by drugs with fewer adverse effects.
A family of DNA-binding transcription factors that contain a basic HELIX-LOOP-HELIX MOTIF.
A growth differentiation factor that plays a role in the neural differentiation, specifically in the retinal development of the EYE.
The main information-processing organs of the nervous system, consisting of the brain, spinal cord, and meninges.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Congenital absence of or defects in structures of the jaw.
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.
Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The complex processes of initiating CELL DIFFERENTIATION in the embryo. The precise regulation by cell interactions leads to diversity of cell types and specific pattern of organization (EMBRYOGENESIS).
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
The hollow, muscular organ that maintains the circulation of the blood.
A method for ordering genetic loci along CHROMOSOMES. The method involves fusing irradiated donor cells with host cells from another species. Following cell fusion, fragments of DNA from the irradiated cells become integrated into the chromosomes of the host cells. Molecular probing of DNA obtained from the fused cells is used to determine if two or more genetic loci are located within the same fragment of donor cell DNA.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Populations of thin, motile processes found covering the surface of ciliates (CILIOPHORA) or the free surface of the cells making up ciliated EPITHELIUM. Each cilium arises from a basic granule in the superficial layer of CYTOPLASM. The movement of cilia propels ciliates through the liquid in which they live. The movement of cilia on a ciliated epithelium serves to propel a surface layer of mucus or fluid. (King & Stansfield, A Dictionary of Genetics, 4th ed)
The observable response an animal makes to any situation.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

FGF8 induces formation of an ectopic isthmic organizer and isthmocerebellar development via a repressive effect on Otx2 expression. (1/4712)

Beads containing recombinant FGF8 (FGF8-beads) were implanted in the prospective caudal diencephalon or midbrain of chick embryos at stages 9-12. This induced the neuroepithelium rostral and caudal to the FGF8-bead to form two ectopic, mirror-image midbrains. Furthermore, cells in direct contact with the bead formed an outgrowth that protruded laterally from the neural tube. Tissue within such lateral outgrowths developed proximally into isthmic nuclei and distally into a cerebellum-like structure. These morphogenetic effects were apparently due to FGF8-mediated changes in gene expression in the vicinity of the bead, including a repressive effect on Otx2 and an inductive effect on En1, Fgf8 and Wnt1 expression. The ectopic Fgf8 and Wnt1 expression domains formed nearly complete concentric rings around the FGF8-bead, with the Wnt1 ring outermost. These observations suggest that FGF8 induces the formation of a ring-like ectopic signaling center (organizer) in the lateral wall of the brain, similar to the one that normally encircles the neural tube at the isthmic constriction, which is located at the boundary between the prospective midbrain and hindbrain. This ectopic isthmic organizer apparently sends long-range patterning signals both rostrally and caudally, resulting in the development of the two ectopic midbrains. Interestingly, our data suggest that these inductive signals spread readily in a caudal direction, but are inhibited from spreading rostrally across diencephalic neuromere boundaries. These results provide insights into the mechanism by which FGF8 induces an ectopic organizer and suggest that a negative feedback loop between Fgf8 and Otx2 plays a key role in patterning the midbrain and anterior hindbrain.  (+info)

The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping. (2/4712)

Left-right asymmetry in vertebrates is controlled by activities emanating from the left lateral plate. How these signals get transmitted to the forming organs is not known. A candidate mediator in mouse, frog and zebrafish embryos is the homeobox gene Pitx2. It is asymmetrically expressed in the left lateral plate mesoderm, tubular heart and early gut tube. Localized Pitx2 expression continues when these organs undergo asymmetric looping morphogenesis. Ectopic expression of Xnr1 in the right lateral plate induces Pitx2 transcription in Xenopus. Misexpression of Pitx2 affects situs and morphology of organs. These experiments suggest a role for Pitx2 in promoting looping of the linear heart and gut.  (+info)

A Drosophila doublesex-related gene, terra, is involved in somitogenesis in vertebrates. (3/4712)

The Drosophila doublesex (dsx) gene encodes a transcription factor that mediates sex determination. We describe the characterization of a novel zebrafish zinc-finger gene, terra, which contains a DNA binding domain similar to that of the Drosophila dsx gene. However, unlike dsx, terra is transiently expressed in the presomitic mesoderm and newly formed somites. Expression of terra in presomitic mesoderm is restricted to cells that lack expression of MyoD. In vivo, terra expression is reduced by hedgehog but enhanced by BMP signals. Overexpression of terra induces rapid apoptosis both in vitro and in vivo, suggesting that a tight regulation of terra expression is required during embryogenesis. Terra has both human and mouse homologs and is specifically expressed in mouse somites. Taken together, our findings suggest that terra is a highly conserved protein that plays specific roles in early somitogenesis of vertebrates.  (+info)

Alzheimer's disease: clues from flies and worms. (4/4712)

Presenilin mutations give rise to familial Alzheimer's disease and result in elevated production of amyloid beta peptide. Recent evidence that presenilins act in developmental signalling pathways may be the key to understanding how senile plaques, neurofibrillary tangles and apoptosis are all biochemically linked.  (+info)

Isolation of zebrafish gdf7 and comparative genetic mapping of genes belonging to the growth/differentiation factor 5, 6, 7 subgroup of the TGF-beta superfamily. (5/4712)

The Growth/differentiation factor (Gdf) 5, 6, 7 genes form a closely related subgroup belonging to the TGF-beta superfamily. In zebrafish, there are three genes that belong to the Gdf5, 6, 7 subgroup that have been named radar, dynamo, and contact. The genes radar and dynamo both encode proteins most similar to mouse GDF6. The orthologous identity of these genes on the basis of amino acid similarities has not been clear. We have identified gdf7, a fourth zebrafish gene belonging to the Gdf5, 6, 7 subgroup. To assign correct orthologies and to investigate the evolutionary relationships of the human, mouse, and zebrafish Gdf5, 6, 7 subgroup, we have compared genetic map positions of the zebrafish and mammalian genes. We have mapped zebrafish gdf7 to linkage group (LG) 17, contact to LG9, GDF6 to human chromosome (Hsa) 8 and GDF7 to Hsa2p. The radar and dynamo genes have been localized previously to LG16 and LG19, respectively. A comparison of syntenies shared among human, mouse, and zebrafish genomes indicates that gdf7 is the ortholog of mammalian GDF7/Gdf7. LG16 shares syntenic relationships with mouse chromosome (Mmu) 4, including Gdf6. Portions of LG16 and LG19 appear to be duplicate chromosomes, thus suggesting that radar and dynamo are both orthologs of Gdf6. Finally, the mapping data is consistent with contact being the zebrafish ortholog of mammalian GDF5/Gdf5.  (+info)

A molecular pathway revealing a genetic basis for human cardiac and craniofacial defects. (6/4712)

Microdeletions of chromosome 22q11 are the most common genetic defects associated with cardiac and craniofacial anomalies in humans. A screen for mouse genes dependent on dHAND, a transcription factor implicated in neural crest development, identified Ufd1, which maps to human 22q11 and encodes a protein involved in degradation of ubiquitinated proteins. Mouse Ufd1 was specifically expressed in most tissues affected in patients with 22q11 deletion syndrome. The human UFD1L gene was deleted in all 182 patients studied with 22q11 deletion, and a smaller deletion of approximately 20 kilobases that removed exons 1 to 3 of UFD1L was found in one individual with features typical of 22q11 deletion syndrome. These data suggest that UFD1L haploinsufficiency contributes to the congenital heart and craniofacial defects seen in 22q11 deletion.  (+info)

Expression of CD44 in Apc and Tcf mutant mice implies regulation by the WNT pathway. (7/4712)

Overexpression of cell surface glycoproteins of the CD44 family is an early event in the colorectal adenoma-carcinoma sequence. This suggests a link with disruption of APC tumor suppressor protein-mediated regulation of beta-catenin/Tcf-4 signaling, which is crucial in initiating tumorigenesis. To explore this hypothesis, we analyzed CD44 expression in the intestinal mucosa of mice and humans with genetic defects in either APC or Tcf-4, leading to constitutive activation or blockade of the beta-catenin/Tcf-4 pathway, respectively. We show that CD44 expression in the non-neoplastic intestinal mucosa of Apc mutant mice is confined to the crypt epithelium but that CD44 is strongly overexpressed in adenomas as well as in invasive carcinomas. This overexpression includes the standard part of the CD44 (CD44s) as well as variant exons (CD44v). Interestingly, deregulated CD44 expression is already present in aberrant crypt foci with dysplasia (ACFs), the earliest detectable lesions of colorectal neoplasia. Like ACFs of Apc-mutant mice, ACFs of familial adenomatous polyposis (FAP) patients also overexpress CD44. In sharp contrast, Tcf-4 mutant mice show a complete absence of CD44 in the epithelium of the small intestine. This loss of CD44 concurs with loss of stem cell characteristics, shared with adenoma cells. Our results indicate that CD44 expression is part of a genetic program controlled by the beta-catenin/Tcf-4 signaling pathway and suggest a role for CD44 in the generation and turnover of epithelial cells.  (+info)

Comparative synteny cloning of zebrafish you-too: mutations in the Hedgehog target gli2 affect ventral forebrain patterning. (8/4712)

Zebrafish you-too (yot) mutations interfere with Hedgehog (Hh) signaling during embryogenesis. Using a comparative synteny approach, we isolated yot as a zinc finger transcription factor homologous to the Hh target gli2. Two alleles of yot contain nonsense mutations resulting in carboxy-terminally truncated proteins. In addition to causing defects in midline development, muscle differentiation, and retinal axon guidance, yot mutations disrupt anterior pituitary and ventral forebrain differentiation. yot mutations also cause ectopic lens formation in the ventral diencephalon. These findings reveal that truncated zebrafish Gli2 proteins interfere with Hh signaling necessary for differentiation and axon guidance in the ventral forebrain.  (+info)

We investigated the mechanisms of intermediate cell mass (ICM) expansion in zebrafish chordin (Chd) morphant embryos and examined the role of BMPs in relation to this phenotype. At 24 h post-fertilization (hpf), the expanded ICM of embryos injected with chd morpholino (MO) (Chd MO embryos) contained a monotonous population of hematopoietic progenitors. In situ hybridization showed that hematopoietic transcription factors were ubiquitously expressed in the ICM whereas vascular gene expression was confined to the periphery. BMP4 (but not BMP2b or 7) and smad5 mRNA were ectopically expressed in the Chd MO ICM. At 48 hpf, monocytic cells were evident in both the ICM and circulation of Chd MO but not WT embryos. While injection of BMP4 MO had no effect on WT hematopoiesis, co-injecting BMP4 with chd MOs significantly reduced ICM expansion. Microarray studies revealed a number of genes that were differentially expressed in Chd MO and WT embryos and their roles in hematopoiesis has yet to be ...
The zebrafish genome contains ten genes that encode class II cytokine-like peptides, of which the two that are related most closely to mammalian interferon gamma (IFN-γ) were named IFN-γ1 and IFN-γ2. Although the zebrafish has become a popular model system to study immune mechanisms, and although interferons are central regulators of immunity, which zebrafish cytokines correspond functionally to mammalian IFN-γ has not been established. We used zebrafish embryos to assay the functions of IFN-γ1 and IFN-γ2, and have identified a subset of zebrafish homologs of the mammalian IFN-responsive genes as IFN-γ targets in the zebrafish embryo: these genes are upregulated in response to raised levels of either IFN-γ1 or IFN-γ2. Infection studies using two different pathogens show that IFN-γ signalling is required for resistance against bacterial infections in the young embryo and that the levels of IFN-γ need to be regulated tightly: raising IFN-γ levels sensitizes fish embryos against ...
Kupffers vesicle (KV) is a spherical left-right organizer which appears transiently during an embryonic stage in zebrafish (1). KV originates from a cluster of dorsal forerunner cells (DFCs). Cadherin1 (Cdh1)-mediated adherens junctions sustain cell cluster formation between the adjacent DFCs and a cluster of DFCs actively migrates towards the vegetal pole to form KV (2). Then, migrated DFCs attach to the overlying surface epithelium and become polarized to construct a rosette-like structure which contains the lumen at the apical point (3). Finally, cilia-formed and fluid-filled KV expands the internal lumen and motile cilia generate fluid flow in a counterclockwise direction to evoke asymmetric signal(s) such as Nodal, Lefty, and Pitx2 (1).. The vertebrate body plan is accomplished by left-right asymmetric organ development. The heart is a representative asymmetric internal organ which jogs to the left-side and proper positioning during embryonic development is crucial for its function (4). ...
and Characterization of zebrafish von Willebrand factor reveals conservation of domain structure, multimerization, and intracellular storage by J. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. Maintaining the Barrier Function of Blood Vessels. I. Zebrafish are a promising model for obesity research, as lipid metabolism pathways are conserved between mammals and fish [44-46].Zebrafish have the key organs that are important for energy homeostasis and metabolism in mammals, as well as other key functions such as appetite regulation in the brain [], insulin regulation [], endocrine signaling through leptin [], and … The present review provides a brief overview of recent studies on wild‐type and transgenic zebrafish as a model system to monitor toxic heavy metals, endocrine disruptors, and organic pollutants for toxicology. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, ...
Interest in using zebrafish is on the rise for several reasons. Zebrafish are small and inexpensive to maintain, and they produce hundreds of offspring in every mating, easily accumulating statistically significant numbers of animals. Whats more, zebrafish embryos are transparent and develop outside the uterus, making it easy to monitor developing fish. In fact, zebrafish embryos are tiny enough to be raised in microplate wells for high-throughput whole-animal assays. Many test compounds can diffuse into zebrafish embryos, simplifying drug administration. Researchers can also readily identify zebrafish with gene mutations. Lastly, as vertebrates, zebrafish and other fish have most of the same organs and organ systems that mammals do, making them more relevant in whole-animal assays than fruit flies or roundworms, which have also been used in early drug discovery screening.. ...
The team identified 154 pseudogenes in the zebrafish genome, a fraction of the 13,000 or so pseudogenes found in the human genome. In fact, 70% of human genes are found in zebrafish . Practically speaking they offer many advantages as research models: The understanding of several human diseases has already grown leaps and bounds because of zebrafish studies. Disruption of enhancer function has been shown to lead to abnormal gene expression and thus to disease (2-4). The genetic underpinnings of heart development in zebrafish are highly similar to that in humans, while zebrafish presents many advantages that allow for rapid screening of … This paper focuses on … Hopkins and her colleagues found that a gene called met, which is known to cause cancer, sits on a chromosome found in excess in zebrafish tumors.,Other researchers had previously observed met on a chromosome found in extra numbers in human cancer. This high degree of similarity has led to the broad use of zebrafish to study the ...
J Vis Exp. 2012 Dec 16;(70). pii: 4243. doi: 10.3791/4243. Chang JT, Sive H. Source Department of Biology, Whitehead Institute of Biomedical Research, Massachusetts Institute of Technology. Abstract Cerebrospinal fluid (CSF) is a protein rich fluid contained within the brain ventricles. It is present during early vertebrate embryonic development and persists throughout life. Adult CSF is thought to cushion the brain, remove waste, and carry secreted molecules(1,2). In the adult and older embryo, the majority of CSF is made by the choroid plexus, a series of highly vascularized secretory regions located adjacent to the brain ventricles(3-5). In zebrafish, the choroid plexus is fully formed at 144 hours post fertilization (hpf)(6). Prior to this, in both zebrafish and other vertebrate embryos including mouse, a significant amount of embryonic CSF (eCSF) is present . These data and studies in chick suggest that the neuroepithelium is secretory early in development and may be the major source of ...
Danio rerio (zebrafish) have rapidly gained popularity in the study of vertebrate development and gene function. Zebrafish are an excellent model for performing large-scale genetic and drug screens to be performed coupled with the visualization of downstream perturbations on live, developing organisms. Due to the fact that large numbers of embryos are required to perform these experiments, we investigated ways to efficiently increase zebrafish embryo production while not being overly invasive or using chemicals that might affect the developmental processes. To perform these experiments, we mated wild type (wt) zebrafish while exposing them to the different wavelengths of light. Fish were exposed to 12 h of dark, followed by 4 h with the different colored light treatments. Once this light cycle was over, embryo production was enumerated. Results indicated that exposing zebrafish to the different wavelengths of fluorescent and LED light had little effect on embryo production. Further research may
The epithelium is the outer most layer of cells responsible for mediating the interactions of an organism with its external environment. Squamous cells are scale-like flattened cells that form the most superficial layer of the epithelium. Regulatory errors in these cells can lead to squamous cell carcinoma, the second most common form of skin cancer. Understanding of the underlying molecular mechanisms is integral for improved diagnostics and therapeutics. IKK1 is a known regulator of several important pathways including regulation of epidermal differentiation and proliferation. Changes in IKK1 expression have been linked to tumor proliferation in squamous cell carcinomas. IKK1 -/- knockout mice have proven critical in understanding the function of IKK1 but are difficult in studies of early epidermal development. The novel zebrafish maternal-effect mutant poky contains a mutation in the zebrafish homolog ikk1. This study focuses on a related protein kinase called Ripk4. The mouse homolog RIP4 is ...
Associate Professor, Biology Department. Syracuse University (Syracuse, New York). Service: I have been actively involved in the zebrafish community for my whole career. Ive attended all but one of the international zebrafish meetings since my first international zebrafish conference in 1996, all of the strategic conferences for zebrafish investigators held in the US and a couple of the European PI meetings. Recently I hosted and co-organized (with Jeff Amack and Jason Myers) the first ever Central New York zebrafish conference. I have been a PhD examiner for many zebrafish theses, an ad hoc reviewer for zebrafish grants for 12 different funding agencies in the US, Europe and Hong Kong and an NSF panel member for IOS Neural Organization. I was an elected committee member for the British Society for Developmental Biology for 5 years and was a scientific organizer for the annual BSDB conference in 2010 and a Frontiers of Science Symposium co-funded by the Royal Society (UK) and Japanese Society ...
TY - JOUR. T1 - K-ras/PI3K-Akt signaling is essential for zebrafish hematopoiesis and angiogenesis. AU - Liu, Lihui. AU - Zhu, Shizhen. AU - Gong, Zhiyuan. AU - Low, Boon Chuan. PY - 2008/8/6. Y1 - 2008/8/6. N2 - The RAS small GTPases orchestrate multiple cellular processes. Studies on knock-out mice showed the essential and sufficient role of K-RAS, but not N-RAS and H-RAS in embryonic development. However, many physiological functions of K-RAS in vivo remain unclear. Using wild-type and fli1:GFP transgenic zebrafish, we showed that K-ras-knockdown resulted in specific hematopoietic and angiogenic defects, including the impaired expression of erythroid-specific gene gatal and βe3-hemoglobin, reduced blood circulation and disorganized blood vessels. Expression of either K-rasC40 that links to phosphoinositide 3-kinase (P13K) activation, or Akt2 that acts downstream of P13K, could rescue both hematopoietic and angiogenic defects in the K-ras knockdown. Consistently, the functional rescue by ...
Welcome to the Zebrafish Welfare Terms Wiki. The zebrafish welfare terms WIKI provides a standardised approach to the language used across facilities when describing zebrafish characteristics, which have the potential to impact welfare. We hope that by using these standardised terms we raise awareness of potential health concerns specific to genetic background and environmental impacts, which affect the well-being of zebrafish in our facilities and help bridge communication between different constituents involved in animal care. The terms have been created, reviewed, and added to by experienced animal caretakers and veterinarians.. If you would like to add to or edit this document please email one of these contacts. Further information regarding zebrafish welfare terms can be found in: Standardised Welfare Terms for the Zebrafish Community ( Goodwin et al_zeb_2016_1248.pdf. ...
The Chi-Bin Chien Award was established by the zebrafish research community and the International Zebrafish Society (IZFS) in memory of Dr. Chi-Bin Chien (1965-2011). Chi-Bin was Professor of Neurobiology and Anatomy at the University of Utah and served the international zebrafish community in numerous ways, including his service as Director of the Zebrafish Neural Development and Genetics Course at the Woods Hole Marine Biological Laboratory and as an organizer of the International Conference on Zebrafish Genetics and Development. The Award celebrates Chi-Bins enthusiasm and love for the discussion of scientific ideas and the good things that grow out of interactions and open discourse among scientists, his efforts to mentor and support the development of young scientists, and the collaborative and generous spirit with which he contributed to advance research with the zebrafish. The award will be given to an outstanding graduate student, postdoctoral trainee, or recently appointed faculty ...
2. Center for Research Informatics, The University of Chicago, 5751 S. Woodlawn Avenue, Chicago, IL 60637, USA. Zebrafish excel as a model organism for developmental biology and disease modeling. Traditionally, laboratory zebrafish have been maintained as outbred populations with high genetic variability. Our recent work has examined the core MHC locus in zebrafish, where we found alternative pathways of antigen processing and presentation genes that are separated by 500 million years of evolution. Here we performed highcoverage genomic sequencing for two clonal lines of zebrafish, and one partially inbred zebrafish, to uncover additional sources of immune gene variation throughout these genomes. Pathway analysis identified immune genes as highly enriched among genes under positive selection, or associated with structural variation. Overall, zebrafish genomes are enriched by approximately 5 fold higher levels of variation compared with humans, including SNVs, small indels, and structural ...
The zebrafish DVR-1 (zDVR-1) gene, like Xenopus Vg1, is present maternally as an unprocessed precursor protein which is distributed ubiquitously along the future dorsoventral axis. Also, like Vg1, overexpression of zDVR-1 in zebrafish directs synthesis of more precursor, but no processed protein. Ho …
INTRODUCTION. Zebrafish maintains a remarkably higher ability than mammals to repair complex tissues after injury, including the heart and the central nervous system. For this reason, zebrafish embryos and their stem cells have been increasingly studied to unravel the molecular mechanisms underlying such regenerative potential, or to dissect evolutionary conserved pathways that may account for the regenerative action afforded by stem cells across different species.. Transplantation of human cord blood-derived CD34+ (hCD34+) cells into pregastrulation zebrafish embryos revealed that these human cells cosegregated with presumptive zebrafish hemangioblasts, being involved in early development of the embryonic vasculature of the recipient 1. Conversely, postgastrulation transplant resulted in the recruitment of hCD34+ cells into developing vessels, where their biology was mainly shifted to a paracrine action 2. These human cells were also found to accelerate vascular repair in adult zebrafish, after ...
MicroRNAs are a well-studied class of non-coding RNA and are known to regulate developmental processes in eukaryotes. Their role in key biological processes such as vasculature development has attracted interest. However, a comprehensive understanding of molecular regulation of angiogenesis and vascular integrity during development remains less explored. Here we identified miRNAs involved in the development and maintenance of vasculature in zebrafish embryos using a reverse genetics approach. Using a combination of bioinformatics predictions and literature based evidences we mined over 701 Human and 329 Zebrafish miRNAs to derive a list of 29 miRNAs targeting vascular specific genes in zebrafish. We shortlisted eight miRNAs and investigated their potential role in regulating vascular development in zebrafish transgenic model. In this screen we identified three miRNAs, namely miR-1, miR-144 and miR-142a-3p that have the potential to influence vascular development in zebrafish. We show that miR-142a-3p
The present study focuses on a cDNA fragment of 2.6 kb coding for glial fibrillary acidic protein (GFAP) related gene in zebrafish. Earlier work in the lab revealed that this gene showed 72% homology, at the amino acid level, with the mammalian gene coding for GFAP. Due to variations observed in sequence of head domain of GFAP and in expression pattern compared to that of rodent GFAP, this gene was named as zebrafish GFAP like gene (zfgfap-l)( Gene Bank Accession No: AY 397679).In this project, the expression pattern of zfgfap-l was studied by RT-PCR and in situ hybridization. RT-PCR results showed that the expression of zfgfap-l started as early as 1.5 hours post-fertilization (hpf) and increased steadily up to 10 hpf, then became a constant level of expression till 30 hpf. In embryos at sphere stage, the expression was detected in the cells of the superficial layer by in situ hybridization. After gastrulation, the expression became restricted to the neural tube, particularly in the presumptive ...
THE zebrafish (Danio rerio) is a powerful tool for understanding vertebrate biology. The usefulness of this model organism is bolstered by the availability of a finished sequenced and annotated genome (Howe et al. 2013; Flicek et al. 2014). As a natural extension of this resource, there are several high-throughput efforts to systematically mutagenize all zebrafish protein-coding genes (Moens et al. 2008; Kettleborough et al. 2013; Varshney et al. 2013a,b).. In addition to such projects, the combination of a sequenced genome and developments in targeted nuclease technology mean that the zebrafish community is now able to rapidly take advantage of custom genome-editing technologies (Doyon et al. 2008; Bedell et al. 2012; Hruscha et al. 2013; Hwang et al. 2013; Jao et al. 2013). CRISPRs in particular provide an efficient, easy, and inexpensive means of manipulating and interrogating the genome (Jinek et al. 2012; Cong et al. 2013; Mali et al. 2013). However, because there are very few hardy ...
Several cardiomyopathy models have been developed in the zebrafish. Unlike other vertebrate models, zebrafish larvae have the unique capacity to oxygenate through diffusion alone for the first 7 dpf. This allows for the study of mutations leading to severe cardiovascular compromise, including those that result in a non-contractile heart. One such mutation, silent heart, was initially identified through a forward genetic screen and subsequently attributed to disruption of the gene TNNT2, encoding the cardiac contractile protein troponin T (Chen et al., 1996; Sehnert et al., 2002; Stainier et al., 1996). Zebrafish expressing the silent heart mutation also demonstrate a reduction in α-tropomyosin and cardiac troponins C and I, resulting in severe sarcomere defects and cardiomyocyte disarray. These ultrastructural changes parallel those seen in human TNNT2 mutations, which lead to familial forms of hypertrophic and dilated cardiomyopathy. Similarly, the pickwickm171 mutation in zebrafish causes a ...
RUNX1/AML1/CBFA2 is essential for definitive hematopoiesis, and chromosomal translocations affecting RUNX1 are frequently involved in human leukemias. Consequently, the normal function of RUNX1 and its involvement in leukemogenesis remain subject to intensive research. To further elucidate the role of RUNX1 in hematopoiesis, we cloned the zebrafish ortholog (runx1) and analyzed its function using this model system. Zebrafish runx1 is expressed in hematopoietic and neuronal cells during early embryogenesis. runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl, and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche. Transient expression of runx1 in cloche embryos resulted in partial rescue of the hematopoietic defect. Depletion of Runx1 with antisense morpholino oligonucleotides abrogated the development of both blood and vessels, as demonstrated by loss of circulation, incomplete development of vasculature ...
During embryogenesis, cells acquire distinct fates by transitioning through transcriptional states. To uncover these transcriptional trajectories during zebrafish embryogenesis, we sequenced 38,731 cells and developed URD, a simulated diffusion-based computational reconstruction method. URD identifi …
Huang H., Lu F.I., Jia S., Meng S., Cao Y., Wang Y., Ma W., Yin K., Wen Z., Peng J., Thisse C., Thisse B., Meng A.. Angiomotin (Amot), the founding member of the Motin family, is involved in angiogenesis by regulating endothelial cell motility, and is required for visceral endoderm movement in mice. However, little is known about biological functions of the other two members of the Motin family, Angiomotin-like1 (Amotl1) and Angiomotin-like2 (Amotl2). Here, we have identified zebrafish amotl2 as an Fgf-responsive gene. Zebrafish amotl2 is expressed maternally and in restricted cell types zygotically. Knockdown of amotl2 expression delays epiboly and impairs convergence and extension movement, and amotl2-deficient cells in mosaic embryos fail to migrate properly. This coincides with loss of membrane protrusions and disorder of F-actin. Amotl2 partially co-localizes with RhoB-or EEA1-positive endosomes and the non-receptor tyrosine kinase c-Src. We further demonstrate that Amotl2 interacts ...
Purpose: Differentiation of diverse photoreceptor phenotypes in the vertebrate retina requires multiple signaling pathways that activate cascades of gene expression. The signaling molecule retinoic acid (RA) is known to regulate rod and cone cell fate, differentiation, and survival. The purpose of the current study is to identify photoreceptor genes controlled by RA signaling in the embryonic retina of the zebrafish.. Methods: We treated embryos with RA at 48 hours post-fertilization (hpf) and isolated total RNA from eyes for microarray analysis at 75 hpf in order to identify genes responding to RA over the period of photoreceptor differentiation. Differentially expressed genes were validated by quantitative RT-PCR (qRT-PCR) and in situ hybridization. Wild-type zebrafish and those carrying an RA signaling reporter transgene (RARE:YFP) were used.. Results: We identified 180 genes with significantly altered gene expression. Of interest was the long wavelength sensitive opsin 1 (LWS1) gene, which ...
We set out to characterize the expression of col2a1b, the zebrafish ortholog of the human COL2A1. In zebrafish, the col2a1a gene has been shown to be expressed in both chondrocytes and perichondrial cells, whereas our preliminary work suggests col2a1b is expressed only in the perichondrium of cartilage elements; thus, col2a1b collagen promotes cartilage development and ossification of chondrocytes. Our lab is interested in understanding the differential expression of the two homologs. In this report, we document the complete spatiotemporal expression of the zebrafish col2a1b mRNA during the first 5 days of development via in situ hybridization.
The early axon scaffolding in the embryonic vertebrate brain consists of a series of ventrally projecting axon tracts that grow into a single major longitudinal pathway connected across the midline by commissures. We have investigated the role of Brother of CDO (BOC), an immunoglobulin (Ig) superfamily member distantly related to the Roundabout (Robo) family of axon-guidance receptors, in the development of this embryonic template of axon tracts in the zebrafish brain. A zebrafish homologue of BOC was isolated and shown to be expressed predominantly in the developing neural plate and later in the neural tube and developing brain. Zebrafish boc was initially highly localized to discrete bands in the mid- and hindbrain, but, as the major brain subdivisions emerged, it became more evenly expressed along the rostrocaudal axis, particularly in dorsal regions. The function of zebrafish boc was examined by a loss-of-function approach. Analysis of embryos injected with antisense morpholinos designed against boc
Midline convergence of organ primordia is an important mechanism for shaping the vertebrate body-plan at various stages of development, such as the morphogenesis of the heart and endoderm. Down regulation of wnt or noncanonical wnt signalling components, such as dishelleved (Dvl) or RhoA GTPase (RhoA), impairs midline convergence of the heart primordia and endoderm in zebrafish. This suggests that wnt signaling plays an important role in regulating midline convergence. At the early patterning stage of the zebrafish kidney, the two pronephric-glomerular primodia (PGP), which derive from intermediate mesoderm, converge towards the midline and fuse to form a functional pronephros. In contrast, during development of the mammalian kidney, the pronephros degenerates as the mesonephros develops without midline convergence. The hypothesis is thus that there is/are mechanisms underlying midline convergence of PGP in zebrafish, which is/are in addition to the control of the non-canonical wnt/Dvl/RhoA ...
The zebrafish has emerged as a premier system for drug discovery. Several small molecules found to be active in zebrafish have been studied in clinical trials. We are at the beginning of an era in which new pathways or chemicals will rescue disease models and lead to new therapeutics, Leonard Zon, M.D., Ph.D., told MSDF. Zon is Grousbeck Professor of Pediatric Medicine at Boston Childrens Hospital and a Howard Hughes Medical Institute investigator. Trede did his postdoctoral work in Zons lab, but Zon was not involved in the LDK study.. At the University of Utah in 2004, Trede began using zebrafish to interrogate a small-molecule library in an effort to identify compounds that kill immature T cells with arrested development. This served as a stand-in for leukemia. He linked the jellyfish gene for green fluorescent protein (GFP) to a zebrafish T cell promoter gene to mark the T cells, which develop in the thymus, easily seen behind a zebrafishs eye. Whenever a T cell lights up green, it ...
The development of new sequencing technologies permits the study of mRNA expression to an unprecedented level. Recent RNA-seq experiments have analysed the changing transcriptome in the early zebrafish embryo (Aanes et al., 2011; Vesterlund et al., 2011; Pauli et al., 2012), but the work described here provides the first systematic identification of maternal and paternal mRNAs in this species. Previous microarray analyses had suggested that transcription occurs before the MZT in the zebrafish (Mathavan et al., 2005) and RNA-seq experiments have also described changes in mRNA levels prior to the MZT, suggesting that such changes were due to the post-transcriptional regulation of mRNAs (Aanes et al., 2011). By identifying zygotically expressed genes (Fig. 2) and then applying tight temporal resolution (Fig. 5B,C), our work confirms that, consistent with previous radioactivity incorporation experiments (Kane and Kimmel, 1993), zygotic transcription begins after ten cell cycles. Through the ...
Hundreds of loci have been robustly associated with circulating lipids, atherosclerosis and coronary artery disease; but for most loci the causal genes and mechanisms remain uncharacterized. The overall aim of my thesis is to develop and validate novel in vivo model systems that are suitable for high-throughput, image-based genetic screens in coronary artery disease and related traits, and use these model systems to systematically characterize positional candidate genes.. In Study I, I developed an experimental pipeline to validate the suitability of zebrafish larvae as a model system for systematic, large-scale characterization of drugs and genes associated with dyslipidemia and atherosclerosis. Using this pipeline, I showed that five days of overfeeding and cholesterol supplementation have independent pro-atherogenic effects in zebrafish larvae, which could be diminished by concomitant treatment with atorvastatin and ezetimibe. CRISPR-Cas9-induced mutations in orthologues of proof-of-concept ...
Hi Jorge, AnaSpec sells rabbit polyclonal anti-SOX9 antibody (cat# 28138) which will work for zebrafish SOX9a. Warm regards, Daniel [Zbrafish] Antibody for SOX9a Zebrafish Jorge Soares via (by j.s.soares from Wed Aug 1 08:07:04 EST 2007 a.. Next message: [Zbrafish] PH meter b.. Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] -------------------------------------------------------------------------------- Hi everyone, Just recently joined this mailing list, mainly because Im searching for an Antibody (mono or poli) that binds to SOX9a from Zebrafish, still leaving the TFBS (transcription Factor Bionding Site) open for it to be able to link to the DNA im studying. Does anyone know of a company that produces it? Cheers. Jorge -------------- next part -------------- An HTML attachment was scrubbed... URL: -- I am using the free version of SPAMfighter. We are a ...
including: Anesthesia and Euthanasia in Zebrafish Guidance on the housing and care of zebrafish Danio Rerio 2011 Katso my s kaloista yleens : NC3Rs - Fish ...
We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain...
Proteoglycans (PGs) modulate numerous signaling pathways during development through binding of their glycosaminoglycan (GAG) side chains to various signaling molecules, including fibroblast growth factors (FGFs). A majority of PGs possess two or more GAG side chains, suggesting that GAG multivalency is imperative for biological functions in vivo. However, only a few studies have examined the biological significance of GAG multivalency. In this report, we utilized a library of bis- and tris-xylosides that produce two and three GAG chains on the same scaffold, respectively, thus mimicking PGs, to examine the importance of GAG valency and chain type in regulating FGF/FGFR interactions in vivo in zebrafish. A number of bis- and tris-xylosides, but not mono-xylosides, caused an elongation phenotype upon their injection into embryos. In situ hybridization showed that elongated embryos have elevated expression of the FGF target gene mkp3 but unchanged expression of reporters for other pathways, ...
Due to its transparency, virtually every brain structure of the larval zebrafish is accessible to light-based interrogation of circuit function. Advanced stimulation techniques allow the activation of optogenetic actuators at different resolution levels, and genetically encoded calcium indicators report the activity of a large proportion of neurons in the CNS. Large datasets result and need to be analyzed to identify cells that have specific properties - e.g. activity correlation to sensory stimulation or behavior. Advances in three-dimensional functional mapping in zebrafish are promising; however the mere coordinates of implicated neurons are not sufficient. To comprehensively understand circuit function, these functional maps need to be placed into the proper context of morphological features and projection patterns, neurotransmitter phenotypes, and key anatomical landmarks. We discuss the prospect of merging functional and anatomical data in an integrated atlas from the perspective of our work on
Recently, chemokine gradients have been directly visualized in vivo in the context of Ccl21‐mediated dendritic cell migration in mice (Weber et al, 2013) and in zebrafish, where Cxcl8 mediates neutrophil migration (Sarris et al, 2012) (Fig 2B). Cxcl8 forms an extracellular, matrix‐bound gradient that extends at least 100 μm around the cell that expresses the chemokine. Interestingly, Cxcl8 protein was detected beyond this local tissue gradient and was found to be enriched along the venous vasculature, which includes the CHT from which Cxcl8 meditates the mobilization of neutrophils into the vasculature (Sarris et al, 2012). Since Cxcl8 binding to the venous vasculature is also required for neutrophil arrest on the blood vessel wall and to facilitate the subsequent extravasation (Middleton et al, 1997), it appears that Cxcl8 acts at several stages of neutrophil recruitment to sites of infection.. Binding of Cxcl8 to the extracellular matrix, or more specifically to heparan sulfate ...
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Mentioning: 6 - Optimization of the spontaneous tail coiling test for fast assessment of neurotoxic effects in the zebrafish embryo using an automated workflow in KNIME® - Eberhard Küster, Stefan Scholz, Riccardo Massei, Elisabet Teixidó, Afolarin O. Ogungbemi
Health, ...Research has shown that mutations in the psen1 gene are common in the ...Professor Pertti Panulas research team at the University of Helsinki ...In the study the zebrafish was used as a model organism. The rapidly d...The lack of Presenilin-1 protein induced a change in the behavior of t...,The,zebrafish,revealed,a,central,regulator,for,the,development,of,the,brain,histamine,system,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
, Atoh7 (zebrafish) blocking peptide, GTX48514-PEP, Applications: Apuri, Blocking, ELISA; Affinity purification, Blocking, ELISA; CrossReactivity: Zebrafish
TY - JOUR. T1 - Zebrafish primordial germ cell cultures derived from vasa::RFP transgenic embryos. AU - Fan, Lianchun. AU - Moon, Jesung. AU - Wong, Ten Tsao. AU - Crodian, Jennifer. AU - Collodi, Paul. PY - 2008/6/1. Y1 - 2008/6/1. N2 - Although embryonic germ (EG) cell-mediated gene transfer has been successful in the mouse for more than a decade, this approach is limited in other species due to the difficulty of isolating the small numbers of progenitors of germ cell lineage (PGCs) from early-stage embryos and the lack of information on the in vitro culture requirements of the cells. In this study, methods were established for the culture of PGCs obtained from zebrafish embryos. Transgenic embryos that express the red fluorescent protein (RFP) under the control of the PGC-specific vasa promoter were used, making it possible to isolate pure populations of PGCs by fluorescence-activated cell sorting (FACS) and to optimize the culture conditions by counting the number of fluorescent PGC colonies ...
The advent of high-throughput sequencing has greatly accelerated the identification of inherited and de novo disease causing mutations. Following discovery the analysis of the developmental and cellular pathways of the affected genes is a crucial step on the path towards therapy with model organisms as the central tool. Traditionally, vertebrate model organisms such as mouse and zebrafish have been used on a gene-by-gene basis, however, in order to keep pace with the increasing speed of discovery, new approaches are needed. Owing to a high quality genome reference sequence and its genetic and embryological tractability the zebrafish is a vertebrate model especially suited for large scale studies. Previously having established methods to generate and identify disruptive zebrafish point mutations on a genome wide scale the Zebrafish Mutation Project is now assigning biological function to every protein-coding gene in the zebrafish genome. We submit alleles to a high-throughput assessment of ...
Swap70 functions as a guanine nucleotide exchange factor for Rac and RhoA regulating F-actin cytoskeletal rearrangements and playing a crucial role in mammalian cell activation, migration, adhesion and invasion. Here we show that the zebrafish orthologue, Swap70b, is required for convergent and extension cell movement during gastrulation. Swap70b morphants exhibited broader and shorter body axis but cell fate specification appeared normal. While ectopic Swap70b expression robustly rescued Wnt11 morphants, RhoA overexpression was sufficient to rescue Swap70b morphants, establishing Swap70b as a novel member of the non-canonical Wnt/PCP pathway downstream of Wnt11 and upstream of RhoA. This is distinct from the related Def6a protein that acts downstream of Wnt5b. Def6a/Swap70b morphants resemble Ppt/Slb double mutant embryos suggesting that Swap70b and Def6a delineate Wnt11 and Wnt5b signalling pathways.. ...
TY - JOUR. T1 - Dna hypomethylation induces a dna replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos. AU - Jacob, Vinitha. AU - Chernyavskaya, Yelena. AU - Chen, Xintong. AU - Tan, Poh Seng. AU - Kent, Brandon. AU - Hoshida, Yujin. AU - Sadler, Kirsten C.. PY - 2015/2/1. Y1 - 2015/2/1. N2 - UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication and is essential for maintaining DNA methylation. uhrf1 mutant zebrafish have global DNA hypomethylation and display embryonic defects, includinga small liver, and theydie as larvae. Wemake the surprising finding that, despite their reduced organ size, uhrf1 mutants express high levels of genes controlling S-phase and have many more cells undergoing DNA replication, as measured by BrdU incorporation. In contrast to wild-type hepatocytes, which are continually dividing during hepatic outgrowth and thus dilute the BrdU label, uhrf1 mutant ...
Zebrafish (Danio rerio) is a well-recognized model for the study of vertebrate developmental genetics, yet at the same time little is known about the transcriptional events that underlie zebrafish embryogenesis. Here we have employed microarray analysis to study the temporal activity of developmentally regulated genes during zebrafish embryogenesis. Transcriptome analysis at 12 different embryonic time points covering five different developmental stages (maternal, blastula, gastrula, segmentation, and pharyngula) revealed a highly dynamic transcriptional profile. Hierarchical clustering, stage-specific clustering, and algorithms to detect onset and peak of gene expression revealed clearly demarcated transcript clusters with maximum gene activity at distinct developmental stages as well as co-regulated expression of gene groups involved in dedicated functions such as organogenesis. Our study also revealed a previously unidentified cohort of genes that are transcribed prior to the mid-blastula transition,
Dear Zebrafish Researchers, Methods in Cell Science has a special Issue devoted to Zebrafish coming out in November at the Society for Neurosciences Annual Meeting, and entitled:Zebrafish as a model system in neurobiology I. It is a diverse Issue, documenting some of the many techniques and exciting experiments, beyond genomics, possible with Zebrafish. I edited this Issue, and the table of contents for Zebrafish as a model system in neurobiology I is shown below. Soren Andersen ---- TABLE OF CONTENTS List of contributors Editorial Soren S. L. Andersen 1) Zebrafish as an experimental model: strategies for developmental and molecular neurobiology studies Authors 2) Neural fate analysis in Zebrafish using olig2 BAC transgenics Authors 3) Neuron-specific gene manipulation in transparent Zebrafish embryos Authors 4) Practical procedures for ectopic induction of gene expression in zebrafish embryos using Bhc-diazo-caged mRNA Authors 5) Identifying axon guidance defects in the embryonic Zebrafish ...
TY - JOUR. T1 - Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. AU - Tseng, Deng Yu. AU - Chou, Ming Yi. AU - Tseng, Yung Che. AU - Hsiao, Chung Der. AU - Huang, Chang Jen. AU - Kaneko, Toyoji. AU - Hwang, Pung Pung. PY - 2009/3/1. Y1 - 2009/3/1. N2 - Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca2+ uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca2+ uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater stimulated whole body Ca2+ influx and zebrafish epithelial Ca2+ channel (zECaC) mRNA expression, while downregulated ...
A large repertoire of gene-centric data has been generated in the field of zebrafish biology. Although the bulk of these data are available in the public domain, most of them are not readily accessible or available in nonstandard formats. One major challenge is to unify and integrate these widely scattered data sources. We tested the hypothesis that active community participation could be a viable option to address this challenge. We present here our approach to create standards for assimilation and sharing of information and a system of open standards for database intercommunication. We have attempted to address this challenge by creating a community-centric solution for zebrafish gene annotation. The Zebrafish GenomeWiki is a wiki-based resource, which aims to provide an altruistic shared environment for collective annotation of the zebrafish genes. The Zebrafish GenomeWiki has features that enable users to comment, annotate, edit and rate this gene-centric information. The credits for contributions
Naturally, the best model organism depends on the biological question, and zebrafish are not ideal for every application. They share about 70% of their genes with humans and 85% of human genes associated with a disease have a homolog in zebrafish ( Howe et al., 2013 ). The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often called a tropical fish although both tropical and subtropical).. This Primer will provide a synopsis of the different cancer models generated by the zebrafish community to date. * Why are zebrafish used in research? Zebrafish (Danio rerio) are now firmly established as a powerful research model for many areas of biology and medicine. Created by. 13 14. Model organisms advantages and disadvantages. As discussed in the previous section, a simple application of this model would be to evaluate drugs useful in ...
Nonsyndromic hearing loss (NSHL) is of great clinical importance, and mutations in the GJB2 gene and the encoded human CONNEXIN 26 (CX26) protein play important roles in the genetic pathogenesis. The CX26 p.R184Q mutation was shown to be a dominant-negative effect in our previous study. Previously, we also demonstrated that zebrafish Cx30.3 is orthologous to human CX26. In the present study, we established transgenic zebrafish models with mutated Cx30.3 specifically expressed in the supporting cells of zebrafish inner ears driven by the agr2 promoter, to demonstrate and understand the mechanism by which the human CX26 R.184 mutation causes NSHL. Our results indicated that significant structural changes in the inner ears of transgenic lines with mutations were measured and compared to wild-type zebrafish. Simultaneously, significant alterations of transgenic lines with mutations in swimming behavior were analyzed with the zebrafish behavioral assay. This is the first study to investigate the functional
In order to understand the developmental mechanisms of posterior body formation in the zebrafish, a fate map of the zebrafish tailbud was generated along with a detailed analysis of tailbud cell movements. The fate map of the zebrafish tailbud shows that it contains tissue-restricted domains and is not a homogeneous blastema. Furthermore, time-lapse analysis shows that some cell movements and behaviors in the tailbud are similar to those seen during gastrulation, while others are unique to the posterior body. The extension of axial mesoderm and the continuation of ingression throughout zebrafish tail development suggests the continuation of processes initiated during gastrulation. Unique properties of zebrafish posterior body development include the bilateral distribution of tailbud cell progeny and the exhibition of different forms of ingression within specific tailbud domains. The ingression of cells in the anterior tailbud only gives rise to paraxial mesoderm, at the exclusion of axial ...
In this study we set out to investigate the transcriptome dynamics of early zebrafish embryogenesis with a comprehensive experimental set-up that interroga
Because of its permeability to small molecules, zebrafish can be used for testing and screening of drugs affecting different biological processes.50-52 Single and multiple compounds tests can be easily and successfully achieved on both zebrafish embryos and adults.53,54 The reason for this resides in the fact that (1) a high quantity of zebrafish embryos (≈5000) can be obtained synchronously, and (2) the small size of the embryos allows them to fit in a 384-well plate and thus allows high-throughput analyses. In this way, libraries of thousands of compounds can be screened for their effects in a reasonable time frame (eg, weeks). Progresses for drug screening in zebrafish embryos are also subjected to automatic readout for phenotypic effects. An automated high-throughput platform for in vivo chemical screenings on zebrafish embryos has been developed aiming for the highest possible throughput and minimization of human error. It includes an automated method for embryo collection and ...
TY - JOUR. T1 - Mouse lefty2 and zebrafish antivin are feedback inhibitors of nodal signaling during vertebrate gastrulation. AU - Meno, Chikara. AU - Gritsman, Kira. AU - Ohishi, Sachiko. AU - Ohfuji, Yasuhisa. AU - Heckscher, Elizabeth. AU - Mochida, Kyoko. AU - Shimono, Akihiko. AU - Kondoh, Hisato. AU - Talbot, William S.. AU - Robertson, Elizabeth J.. AU - Schier, Alexander F.. AU - Hamada, Hiroshi. PY - 1999/9. Y1 - 1999/9. N2 - Mammalian lefty and zebrafish antivin form a subgroup of the TGFβ superfamily. We report that mouse mutants for lefty2 have an expanded primitive streak and form excess mesoderm, a phenotype opposite to that of mutants for the TGFβ gene nodal. Analogously, overexpression of Antivin or Lefty2 in zebrafish embryos blocks head and trunk mesoderm formation, a phenotype identical to that of mutants caused by loss of Nodal signaling. The lefty2 mutant phenotype is partially suppressed by heterozygosity for nodal. Similarly, the effects of Antivin and Lefty2 can be ...
Zebrafish, Danio rerio (Hamilton 1822), have become a valuable model for investigating the development and molecular genetics of the vertebrate inner ear (Whitfield, 2002; Nicolson, 2005). The early development of the zebrafish inner ear is similar to that of other vertebrates (Bang et al., 2001; Whitfield et al., 2002; Riley and Phillips, 2003) and its sensory hair cells are homologous to those found in mammals (Coffin et al., 2004). Over 50 genes are known to impact the zebrafish auditory inner ear and/or vestibular system (Granato et al., 1996; Whitfield et al., 1996; Whitfield et al., 2002; Riley and Phillips, 2003; Starr et al., 2004; Nicolson, 2005) and many of these genes are conserved and affect the inner ear development and function in other vertebrates, including humans (Nicolson et al., 1998; Moorman et al., 1999; Riley and Moorman, 2000; Busch-Nentwich et al., 2004; Kappler et al., 2004; Kozlowski et al., 2005). However, unlike mammals, zebrafish develop from eggs ex utero and are ...
During early vertebrate development, various small non-coding RNAs (sRNAs) such as MicroRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs) are dynamically expressed for orchestrating the maternal-to-zygotic transition (MZT). Systematic analysis of expression profiles of zebrafish small RNAome will be greatly helpful for understanding the sRNA regulation during embryonic development. We first determined the expression profiles of sRNAs during eight distinct stages of early zebrafish development by sRNA-seq technology. Integrative analyses with a new computational platform of CSZ (characterization of small RNAome for zebrafish) demonstrated an sRNA class transition from piRNAs to miRNAs as development proceeds. We observed that both the abundance and diversity of miRNAs are gradually increased, while the abundance is enhanced more dramatically than the diversity during development. However, although both the abundance and diversity of piRNAs are gradually decreased, the diversity was firstly increased then
Zebrafish is increasingly used a genetic model organism in biomedical studies. This protocol provides a detailed procedure about the identification of the genotype of an adult zebrafish or a zebrafish embryo.
Objectives: Phospholipase C (PLC) gamma 1 has been shown to mediate signal transduction of tyrosine kinases and affect function of hematopoietic cells. However, its role in hematopoiesis during embryonic development is currently unclear. In this study, we examined this issue using morpholino (MO) gene knockdown in zebrafish embryos. Methods: MO targeting at the exon-1-intron-1 junction of zebrafish PLC-γ1 was injected into embryos at the one- to four-cell stage (referred herein zPLC-γ1MO embryos). Primitive hematopoiesis was examined quantitatively by flow cytometry in Tg(gata1:GFP) embryos and by real-time quantitative polymerase chain reaction at 18 hours-post-fertilization (hpf), before the onset of circulation. The embryos were also treated with receptor inhibitors of vascular endothelial growth factor, fibroblast growth factor, and platelet-derived growth factor at 25, 1, and 30 μmol/L, respectively, from one cell until 48 hpf. Results: Erythropoiesis was reduced in zPLC-γ1MO embryos, ...
We investigated the role of the WW domain-containing oxidoreductase (wwox) gene in the embryonic development of zebrafish, with particular emphasis on intracellular Ca2+ dynamics because Ca2+ is an important intracellular messenger. Comparisons between zebrafish wwox and human WWOX sequences identified highly conserved domain structures. wwox was expressed in developing heart tissues in the zebrafish embryo. Moreover, wwox knockdown induced pericardial edema with similarities to conditions observed in human breast cancer. The wwox knockdown embryos with the edema died within a week. High Ca2+ levels were observed at the boundary between the edema and yolk in wwox knockdown embryos.
We recently identified Rbm24 as a novel gene expressed during mouse cardiac development. Due to its tightly restricted and persistent expression from formation of the cardiac crescent onwards and later in forming vasculature we posited it to be a key player in cardiogenesis with additional roles in vasculogenesis and angiogenesis. To determine the role of this gene in cardiac development, we have identified its zebrafish orthologs (rbm24a and rbm24b), and functionally evaluated them during zebrafish embryogenesis. Consistent with our underlying hypothesis, reduction in expression of either ortholog through injection of morpholino antisense oligonucleotides results in cardiogenic defects including cardiac looping and reduced circulation, leading to increasing pericardial edema over time. Additionally, morphant embryos for either ortholog display incompletely overlapping defects in the forming vasculature of the dorsal aorta (DA), posterior caudal vein (PCV) and caudal vein (CV) which are the first blood
TY - JOUR. T1 - Positional cloning of a temperature-sensitive mutant emmental reveals a role for Sly1 during cell proliferation in zebrafish fin regeneration. AU - Nechiporuk, Alex. AU - Poss, Kenneth D.. AU - Johnson, Stephen L.. AU - Keating, Mark T.. N1 - Funding Information: We are grateful to the members of Stephen Johnsons lab for technical advice during the mutagenesis screen. We thank Jennifer Sheppard, Janet Finney, Ann Hillam, Lindsay Wilson, Angela Sanchez, and Soo Kim for excellent fish care. We also thank the members of the Electron Microscopy core facilities at the Department of Cell Biology, Harvard Medical School for help with transmission electron microscopy. K.D.P. was supported by a postdoctoral fellowship from the Helen Hay Whitney Foundation. S.L.J. was supported by NIH PO1 Grant HD39952.. PY - 2003/6/15. Y1 - 2003/6/15. N2 - Here, we used classical genetics in zebrafish to identify temperature-sensitive mutants in caudal fin regeneration. Gross morphological, histological, ...
TY - JOUR. T1 - The catalytic activity and secretion of zebrafish RNases are essential for their in vivo function in motor neurons and vasculature. AU - Ferguson, Ross. AU - Holloway, Daniel E. AU - Chandrasekhar, Anand. AU - Acharya, K Ravi. AU - Subramanian, Vasanta. PY - 2019/2/1. Y1 - 2019/2/1. N2 - Angiogenin (hANG), a member of the Ribonuclease A superfamily has angiogenic, neurotrophic and neuroprotective activities. Mutations in hANG have been found in patients with Amyotrophic lateral sclerosis (ALS). The zebrafish (Danio rerio) rnasel-1, 2 and 3 are orthologues of hANG and of these only Rnasel-1 and Rnasel-2 have been shown to be angiogenic. Herein we show that NCI-65828, a potent and specific small molecule inhibitor of hANG inhibits Rnasel-1 to a similar extent. Treatment of early zebrafish embryos with NCI-65828, or with terrein, a fungal metabolite which prevents the secretion of hANG, resulted in spinal neuron aberrations as well defects in trunk vasculature. Our detailed ...
Basic research in pattern formation is concerned with the generation of phenotypes and tissues. It can therefore lead to new tools for medical research. These include phenotypic screening assays, applications in tissue engineering, as well as general advances in biomedical knowledge. Our aim here is to discuss this emerging field with special reference to tools based on zebrafish developmental biology. We describe phenotypic screening assays being developed in our own and other labs. Our assays involve: (i) systemic or local administration of a test compound or drug to zebrafish in vivo; (ii) the subsequent detection or readout of a defined phenotypic change. A positive readout may result from binding of the test compound to a molecular target involved in a developmental pathway. We present preliminary data on assays for compounds that modulate skeletal patterning, bone turnover, immune responses, inflammation and early-life stress. The assays use live zebrafish embryos and larvae as well as adult
The effect of the nanoparticles on the marine organisms, depends on their size, chemical composition, surface structure, solubility and shape. In order to take advantage from their activity, preserving the surrounding environment from a possible pollution, we are trying to trap the nanoparticles into new nanomaterials. The nanomaterials tested were synthesized proposing a ground-breaking approach by an upside-down vision of the Au/TiO2nano-system to avoid the release of nanoparticles. The system was synthesized by wrapping Au nanoparticles with a thin layer of TiO2. The non-toxicity of the nano-system was established by testing the effect of the material on zebrafish larvae. Danio rerio o zebrafish was considered a excellent model for the environmental biomonitoring of aquatic environments and the Zebrafish Embryo Toxicity Test is considered an alternative method of animal test. For this reason zebrafish larvae were exposed to different concentrations of nanoparticles of TiO2 and Au and new ...
Cell-cell adhesion is an essential component of a multicellular system. Cadherins are a type of molecule that are known for their role in cell adhesion (Takeichi, 1991). Fat are a class of cadherins that have been shown to be responsible in planar cell polarity and epithelial cell division (Dunne et al., 1995; Viktorinová et al., 2009). Fat has gone primarily unstudied within the zebrafish model, with only fat1 being examined for expression (Down et al., 2005). Based on the 34 cadherin repeat motif of Fat genes, predicted sequences for Fat1-4 exist on online databases. In this study, the predicted Fat genes in zebrafish were examined for gene expression patterns during early developmental stages. All genes were expressed at some point in development, confirming their transcription into RNA and no longer classifying them as predicted sequences. One putative homolog of fat3 was selected for antisense morpholino oligonucleotide knockdown. Zebrafish with the fat3 knockdown did not develop hair cells,
The notochord is a midline mesodermal structure with an essential patterning function in all vertebrate embryos. Zebrafish floating head (flh) mutants lack a notochord, but develop with prechordal plate and other mesodermal derivatives, indicating that flh functions specifically in notochord development. We show that floating head is the zebrafish homologue of Xnot, a homeobox gene expressed in the amphibian organizer and notochord. We propose that flh regulates notochord precursor cell fate.
Purpose:: The zebrafish mutant, golden, is hypopigmented due to a mutation in a novel cation exchanger, NCKX5 (SLC24A5). Pigmentation has been shown, at least epidemiologically, to be an important factor in age-related macular degeneration (AMD). In addition, NCKX5 has been demonstrated in human RPE, and the gene encoding NCKX5 is located on chromosome 15q21, a region that also has a major susceptibility locus associated with AMD. The aim of this study is to evaluate the morphologic features of the retina/RPE of the golden zebrafish in comparison with human AMD in order to establish a possible animal model for this disease. Methods:: Wildtype (Lyles) and mutant (golden, golb1) zebrafish (sacrificed in light) were fixed in paraformaldehyde or glutaraldehyde. Eyes were removed and investigated by light (LM) or electron microscopy (EM), respectively. 22 eyes were obtained from wildtype (wt), age range 8-34 months, and 18 from golden, age range 9-33 months. Results:: By LM, there was an obvious ...
The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating and chemically exposing zebrafish embryos-namely, manual placement of embryos into 96- or 384-well plates and exposure of embryos while still in the chorion, a barrier of poorly characterized permeability enclosing the developing embryo. We report the automated dechorionation of 1600 embryos at once at 4 h postfertilization (hpf) and placement of the dechorionated embryos into 96-well plates for exposure by 6 hpf. The process removed ≥95% of the embryos from their chorions with 2% embryo mortality by 24 hpf, and 2% of the embryos malformed at 120 hpf. The robotic embryo placement allocated 6-hpf embryos to 94.7% ± 4.2% of the wells in multiple 96-well trials. The rate of embryo mortality was 2.8% (43 of 1536) from robotic handling, the rate of missed wells was 1.2% (18 of 1536), and the frequency of multipicks was ,0.1%. Embryo malformations observed at 24 hpf ...
Here we describe the use of ChIP coupled to next-generation high-throughput sequencing to identify Hif-1α binding sites throughout the zebrafish genome in the vhl mutant. ChIP allows unbiased genome-wide coverage of the zebrafish genome to identify Hif-1α binding sites, enabling development of zebrafish as an emergent model organism for research into the hypoxic response in a physiological context [41, 42, 46, 52, 68]. The use of the vhl mutant, a zebrafish model for hypoxia, has allowed us to compare Hif-1α binding sites with gene expression changes seen in this mutant.. Microarray analysis of gene expression changes in vhl mutants compared against wild-type controls produced both expected and unexpected results. The up-regulated genes included many classical hypoxia response genes that are associated with loss of Vhl in mammalian cell lines (Additional file 1). This reinforces the idea that the hypoxic response is activated in the vhl mutant. Contrary to this, we see similar numbers of ...
TY - JOUR. T1 - Chapter 19 Zebrafish Informatics and the ZFIN Database. AU - Westerfield, Monte. AU - Doerry, Eckehard. AU - Kirkpatrick, Arthur E.. AU - Douglas, Sarah A.. PY - 1998/1/1. Y1 - 1998/1/1. N2 - The recent increase in the number of research studies using zebrafish to analyze vertebrate development and genetics has led to a significant information access problem. A concerted effort has begun to establish and maintain a centralized database for the zebrafish research community. Because of the dramatic increase in the amount of data and the need for more sophisticated search methods, the Web site information and other zebrafish research information have been integrated into a relational database called the Zebrafish Information Network (ZFIN). The ultimate usefulness and usability of the database depend on a careful assessment of the requirements of the users, early and continued testing of prototype interfaces by real users, and analysis of the users interactive behavior while ...
TY - JOUR. T1 - Skeletal biology and disease modeling in zebrafish. AU - Dietrich , Kristin. AU - Fiedler, Imke. AU - Kurzyukova , Anastasia. AU - López-Delgado , Alejandra Cristina. AU - Mcgowan, Lucy M. AU - Geurtzen , Karina. AU - Hammond, Chrissy. AU - Busse, Björn AU - Knopf, Franziska. PY - 2021/3. Y1 - 2021/3. N2 - Zebrafish are teleosts (bony fish), which share with mammals a common ancestor belonging to the phylum Osteichtyes, from which their endoskeletal systems have been inherited. Indeed, teleosts and mammals have numerous genetically conserved features in terms of skeletal elements, ossification mechanisms and bone matrix components in common. Yet, differences related to bone morphology and function need to be considered when investigating zebrafish in skeletal research. In this review, we focus on zebrafish skeletal architecture with emphasis on the morphology of the vertebral column and associated anatomical structures. We provide an overview on the different ossification types ...
CONSIDERING the fundamental importance of sex for species propagation, it is surprising that primary sex-determining mechanisms are not strongly conserved among animal taxa (Bull 1983; Charlesworth 1996; Ming et al. 2011; Bachtrog et al. 2014). Closely related species or even populations of the same species can have different sex-determining mechanisms (Takehana et al. 2007; Ross et al. 2009; Kobayashi et al. 2013; Heule et al. 2014; Larney et al. 2014). Zebrafish (Danio rerio) is a popular model for studies of vertebrate development, behavior, physiology, evolution, disease, and human health (Mills et al. 2007; Seth et al. 2013; Braasch et al. 2014; Ota and Kawahara 2014; Wilkinson et al. 2014), but researchers struggle with highly variable and distorted sex ratios, and investigations into the genetic nature of zebrafish sex determination are conflicting. To help understand these issues, we conducted a population genomic study of sex determination in multiple zebrafish strains.. Zebrafish ...
Transposable Elements (TEs) are mobile, selfish genes that have, in some cases, been shown to provide functions for the host. A poorly characterized family of LTR retrotransposons, BHIKHARI (bik), has unusual early embryonic, cell-specific expression patterns in the zebrafish (Danio rerio). This led me to investigate their possible role in the development of the zebrafish embryo and their evolutionary history in related species. I present a successful CRISPRi knockdown experiment of bik-1, one of five subfamilies of bik, that shows evidence of the involvement of bik-1 in cell differentiation. Polymorphism analysis gave evidence that bik-1 may still be active in zebrafish while the other bik subfamilies may not be. Sequence analysis of the subfamilies suggests that all five may have arisen from a common element sometime after the divergence of the Danionella and Danio genera. Additionally, I characterize bik elements as members of the recently described Lokiretrovirus clade of retroelements ...
The IZFS was formed as the successor to the Society for Zebrafish Research (SZR), an entity that provided a framework for funding and grant support for the International Conferences on Zebrafish Development and Genetics and other conferences.. © International Zebrafish Society. All Rights Reserved.. ...
In this report, we use zebrafish as a model system to understand the importance of CD146 in vascular development. Endothelial-specific expression of CD146 was verified by whole-mount in situ hybridization. Suppression of CD146 protein expression by antisense morpholino oligonucleotides (MO) resulted in poorly developed intersomitic vessels (ISVs). In CD146 morphants, we observed a lack of blood flow through the ISV region, despite that fluorescence microangiography showed that the ISVs were present. This finding suggests that the lumens of the developing ISVs may be too narrow for proper circulation. Additionally, remodeling of the caudal vein plexus into functional vascular tubes appeared to be affected. Suppression of CD146 protein expression resulted in a circulation shunt that caused the circulation to by-pass part of the caudal artery/vein system. The same vascular defects were recapitulated by using a second morpholino oligonucleotide. This morphant expressed a truncated CD146 protein with ...
Regulatory T (Treg) cells play a central role in the suppression of excessive immune responses against both self and non-self antigens. The development and function of Treg cells are controlled by a master regulatory gene encoding the forkhead box P3 (FOXP3) protein in mammals. However, little is known regarding the functions of Treg cells and FOXP3 in non-mammalian vertebrates. In this study, we generated mutant zebrafish lacking a functional FOXP3 ortholog, and demonstrated a significant reduction in survival accompanied by a marked increase in inflammatory gene expression, mononuclear cell infiltration, and T cell proliferation in peripheral tissues. Our findings indicate that the zebrafish FOXP3 protein may have an evolutionally conserved role in the control of immune tolerance, illuminating the potential of the zebrafish as a novel model for investigating the development and functions of Treg cells.. ...
The fishs development follows a very similar process to the formation one of the long bones in our arm, Akimenko says.. Both genes code for proteins that are part of a pathway that controls the activity of the so-called Hox11 proteins, Hawkins found. In mammals, these proteins guide the formation of the two bones of the forearm. In fish, Hox11 is normally suppressed by these other proteins, but when they are mutated, the body starts to build a forearm. These mutations reawakened a developmental pathway in zebrafish that was hidden, says Frank Tulenko, an evolutionary developmental biologist at Monash University.. Until now, few scientists could have guessed that vav2 and wasl play a role in bone formation. That they do is changing the paradigm on limb development and evolution, says Renata Freitas, a developmental biologist at the University of Porto who calls the study a landmark.. The genetic pathway itself is likely ancient. The fact that it exists in zebrafish, which come from a ...
TY - THES. T1 - Functional Characterization of Protein -Tyrosine Phosphatases in Zebrafish Development using Image Analysis. AU - Runtuwene, V.J.. N1 - Reporting year: 2012. PY - 2012. Y1 - 2012. M3 - PhD thesis (Proefschrift). ER - ...
General information:. The zebrafish newsgroup is a moderated, online discussion group for anyone interested in zebrafish research. Posted messages will first go to the newsgroups moderator (currently Jonathan Knight at ZFIN). If deemed appropriate for the newsgroup, the message will be forwarded to the full subscription list via e-mail and posted on the newsgroup site. Please note that the newsgroup is not monitored evenings or weekends, so messages posted outside of normal business hours in the Pacific time zone in the US will be delayed until the next business day.. Recent topics on the newsgroup include protocols and techniques, water quality and disease issues, and information on suppliers, job openings and funding opportunities. We encourage you to get connected with your colleagues through the zebrafish newsgroup!. Subscribing and Participating. Google Groups is a convenient way to access the zebrafish newsgroup on the web. See this URL: ...
Won M, Ro H, Dawid IB. Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas. Proc Natl Acad Sci U S A. 2015 Sep
The Zebrafish Information Network (ZFIN) is the database of genetic and genomic data for the zebrafish (Danio rerio) as a model organism. ZFIN provides a wide array of expertly curated, organized and cross-referenced zebrafish research data.. ...
ETS domain transcription factors have been linked to hematopoiesis, vasculogenesis, and angiogenesis. However, their biological functions and the mechanisms of action, remain incompletely understood. Here, we have performed a systematic analysis of zebrafish ETS domain genes and identified 31 in the genome. Detailed gene expression profiling revealed that 12 of them are expressed in blood and endothelial precursors during embryonic development. Combined with a phylogenetic tree assay, this suggests that some of the coexpressed genes may have redundant or additive functions in these cells. Loss-of-function analysis of 3 of them, erg, fli1, and etsrp, demonstrated that erg and fli1 act cooperatively and are required for angiogenesis possibly via direct regulation of an endothelial cell junction molecule, VE-cadherin, whereas etsrp is essential for primitive myeloid/endothelial progenitors (hemangioblasts) in zebrafish. Taken together, these results provide a global view of the ETS genes in the zebrafish
The zebrafish (Danio rerio) is an rising mannequin system in a number of analysis areas worldwide, particularly within the International South. On this context, the current examine revised the historic use and traits of zebrafish as experimental fashions in Brazil. The information regarding the bibliometric parameters, analysis areas, geographic distribution, experimental design, zebrafish pressure, and reporter traces, in addition to current advances have been.... ...
The zebrafish (Danio rerio) is an rising mannequin system in a number of analysis areas worldwide, particularly within the International South. On this context, the current examine revised the historic use and traits of zebrafish as experimental fashions in Brazil. The information regarding the bibliometric parameters, analysis areas, geographic distribution, experimental design, zebrafish pressure, and reporter traces, in addition to current advances have been.... ...
... the PGCs express two CXCR4 transmembrane receptor proteins. The signaling system involving this protein and its ligand, Sdf1, ... Regardless of PGC injection site, PGCs are able to correctly migrate to their target sites.[citation needed] In zebrafish, ... Bone morphogenetic protein 4 (BMP4) is released by the extra-embryonic ectoderm (ExE) at embryonic day 5.5 to 5.75 directly ... December 2017). "The Vertebrate Protein Dead End Maintains Primordial Germ Cell Fate by Inhibiting Somatic Differentiation". ...
Tallafuss, Alexandra; Trepman, Alissa; Eisen, Judith S. (2009-12-01). "DeltaA mRNA and protein distribution in the zebrafish ... This can be a gene co-expression network, or a protein-protein interaction (PPI) network. The nodes of the network will be ... Several of these protein disrupting mutations have been able to be identified only with the aid of whole genome sequencing ... The zebrafish model system has already been used to study neuroregeneration and severe polygenic human diseases like cancer and ...
"Differential calcium signaling by cone specific guanylate cyclase-activing proteins from the zebrafish retina". PLoS ONE. 6 (8 ... In addition, studies have shown that zebrafish express a higher number of GCAPs than mammals, and that zebrafish GCAPs can bind ... It is often part of the G protein signaling cascade that is activated by low intracellular calcium levels and inhibited by high ... Depending on cell type, it can drive adaptive/developmental changes requiring protein synthesis. In smooth muscle, cGMP is the ...
"Patterning the zebrafish diencephalon by the conserved zinc-finger protein Fezl". Development. 134 (1): 127-36. doi:10.1242/dev ... In zebrafish, it was shown that the expression of two SHH genes, SHH-a and SHH-b (formerly described as twhh) mark the MDO ... "Hedgehog signalling from the zona limitans intrathalamica orchestrates patterning of the zebrafish diencephalon". Development. ...
Kelly, Gregory M; Reversade, Bruno (1997). "Characterization of a cDNA encoding a novel band 4.1-like protein in zebrafish". ... In 2005, Reversade and De Robertis detailed how multiple extracellular proteins allow embryos that are cut in two to self- ... "Scoperta la proteina che contrasta l'invecchiamento precoce della pelle" [The protein that counteracts premature aging of the ... a G protein-coupled receptor). The genetic inactivation of ELA leads to cardiovascular defects, predisposes to preeclampsia and ...
Currie PD, Ingham PW (August 1996). "Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish". Nature ... SHH is located on chromosome seven and initiates the production of Sonic Hedgehog protein. This protein sends short- and long- ... "Zebrafish SHHa". University of Oregon. Archived from the original on 2009-06-25. "Zebrafish SHHb". University of Oregon. ... Vitronectin is another protein that is induced by SHH; it acts as an obligate co-factor for SHH signaling in the neural tube. ...
... is tied to liver development in an orthologous protein in zebrafish. The function of the human UPF0762 is not yet well ... DUF781 is the singular domain of the protein and spans 318 of the protein's 330 amino acids. DUF781 has been linked to liver ... "liver-enriched gene 1a and 1b encode novel secretory proteins essential for normal liver development in zebrafish". PLOS ONE. 6 ... "Analysis of vaccinia virus-host protein-protein interactions: validations of yeast two-hybrid screenings". J. Proteome Res. 8 ( ...
... "nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate". Development. ... Protein kinase C-interacting protein 1 (PKC1) associates with MITF. Their association is reduced upon cell activation. When ... Microphthalmia-associated transcription factor also known as class E basic helix-loop-helix protein 32 or bHLHe32 is a protein ... Most transcription factors function in cooperation with other factors by protein-protein interactions. Association of MITF with ...
... a newly identified pleckstrin homology domain protein, is required for cardiac contractility in zebrafish". Disease Models & ... The protein PDZD11 was identified as a protein interacting through its N-terminal region with the N-terminal WW domain of ... The protein was discovered in Masatoshi Takeichi's lab while looking for potential binding partners for the N-terminal region ... Tille JC, Ho L, Shah J, Seyde O, McKee TA, Citi S (2015-01-01). "The Expression of the Zonula Adhaerens Protein PLEKHA7 Is ...
Morpholino knockdown of MagT1 and TUSC3 protein expression in zebrafish embryos resulted in early developmental arrest; excess ... They designated this protein, MagT1. MagT1 is expressed as a 335 amino acid polypeptide which includes five transmembrane ... Knockdown of either MagT1 or TUSC3 protein lowered the total and free intracellular Mg2+concentrations in mammalian cell lines ... Recent evidence demonstrates that the primary function of MagT1 is protein glycosylation, mediated by MagT1's function as a ...
In this study, scientists used zebrafish to examine the motion of proteins within live ear cells using a confocal microscope. ... Fascin 2b, a protein involved in actin cross-linking, moves even faster. Constant movement of proteins within cells, along with ... This has shown that proteins in stereocilia move quickly, indicating that the movement of the proteins within the hair cells ... The fast movement of these proteins has changed our understanding of stereocilia and indicates that proteins within stereocilia ...
"Biochemical and cellular characteristics of the four splice variants of protein kinase CK1alpha from zebrafish (Danio rerio)". ... The AD hallmark proteins tau in NFTs or GVBs and TAR DNA-binding protein of 43 kDa (TDP-43) in GVBs colocalize with CK1δ. In ... So far, C-terminal phosphorylation of CK1δ by upstream kinases has been confirmed for protein kinase A (PKA), protein kinase B ... Locasale JW, Shaw AS, Chakraborty AK (August 2007). "Scaffold proteins confer diverse regulatory properties to protein kinase ...
It encodes a protein showing 95%, 86%, and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, ... Protein Wnt-8b is a protein that in humans is encoded by the WNT8B gene. The WNT gene family consists of structurally related ... These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate ... Smolich BD, McMahon JA, McMahon AP, Papkoff J (1994). "Wnt family proteins are secreted and associated with the cell surface". ...
Two other family members that are not obvious orthologs of any identified mammalian FXYD protein exist in zebrafish. All these ... The FXYD protein family is a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning ... FXYD proteins appear to preferentially associate with Na,K-ATPase alpha1-beta isozymes, and affect their function in a way that ... FXYD proteins are widely distributed in mammalian tissues with prominent expression in tissues that perform fluid and solute ...
"Mutations in the microtubule-associated protein MAP11 (C7orf43) cause microcephaly in humans and zebrafish". Brain: A Journal ... "The 60-kDa heat shock protein modulates allograft rejection". Proceedings of the National Academy of Sciences of the United ... A microcephaly syndrome caused by mutations in the microtubule-associated protein MAP11 (C7orf43, TRAPPC14, MCPH25). ...
In zebrafish, the interconnectivity of proteins over developmental time was found to be highest in early development, ... re-analyzed the zebrafish dataset published by Domazet-Lošo and Tautz. They found that applying a log-transformation to the ... In zebrafish, as well as in additional transcriptomic datasets of Drosophila, the mosquito Anopheles and the nematode ... Gene knockout experiments from mice and zebrafish demonstrated that the ratio of essential genes to non-essential genes ...
According to the study, a specialised protein prevents paralysing glial scars forming when zebrafish suffer spinal cord damage ... The protein helps prime stem cells to become specialized cells in mice. (Science Daily) (Cell Stem Cell) Analysts report that ... This process is described as "a step along the path toward amino acids and nucleotides, the raw materials of proteins and DNA, ... It is hoped that this protein may be exploited for the treatment of paralysed humans. (Sci-News) (J. Neurosci.) 4 June - A ...
"A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion ... which function as activators of small G proteins. Dock5 is predicted to activate the small G protein Rac. Dock5 shares ... Meller N, Merlot S, Guda C (2005). "CZH proteins: a new family of Rho-GEFs". J. Cell Sci. 118 (Pt 21): 4937-46. doi:10.1242/jcs ... Côté JF, Vuori K (2007). "GEF what? Dock180 and related proteins help Rac to polarize cells in new ways". Trends Cell Biol. 17 ...
Several groups have been developing zebrafish to detect pollution by attaching fluorescent proteins to genes activated by the ... There is also potential to use the silk producing machinery to make other valuable proteins. Proteins expressed by silkworms ... Human proteins expressed in mammals are more likely to be similar to their natural counterparts than those expressed in plants ... "Green fluorescent protein takes Nobel prize". Lewis Brindley. Retrieved 2015-05-31. Alberts B, Johnson A, Lewis J, Raff M, ...
Several groups have been developing zebrafish to detect pollution by attaching fluorescent proteins to genes activated by the ... The majority of these products are human proteins for use in medicine. Many of these proteins are impossible or difficult to ... There is also potential to use the silk producing machinery to make other valuable proteins. Proteins currently developed to be ... Human proteins expressed in mammals are more likely to be similar to their natural counterparts than those expressed in plants ...
"Green fluorescent protein (GFP) as a marker of aryl hydrocarbon receptor (AhR) function in developing zebrafish (Danio rerio)" ... For example, a gene from a jellyfish, encoding a fluorescent protein called GFP, or green fluorescent protein, can be ... 2000). "Transgenic zebrafish as sentinels for aquatic pollution". Ann N Y Acad Sci. 919: 133-47. Bibcode:2000NYASA.919..133C. ... Gasdaska JR et al. (2003) "Advantages of Therapeutic Protein Production in the Aquatic Plant Lemna". BioProcessing Journal Mar/ ...
It was originally identified during a search for immune system genes in zebrafish as a protein coding sequence related to APAF1 ... NWD1, short for NACHT and WD repeat domain containing 1, is a gene found in vertebrates, which encodes a protein that contains ... Correa RG, Krajewska M, Ware CF, Gerlic M, Reed JC (2014). "The NLR-related protein NWD1 is associated with prostate cancer and ... "Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish". Genome Biol. ...
... with homology to an Escherichia coli and a zebrafish protein that maps to chromosome 21q22.3". Hum Genet. 99 (5): 616-23. doi: ... 1996). "Isolation of cDNA for a novel human protein KNP-I that is homologous to the E. coli SCRP-27A protein from the ... Glutamine amidotransferase-like class 1 domain-containing protein 3A, mitochondrial is a protein that in humans is encoded by ... 1993). "Human liver protein map: a reference database established by microsequencing and gel comparison". Electrophoresis. 13 ( ...
"The TATA-binding protein regulates maternal mRNA degradation and differential zygotic transcription in zebrafish". The EMBO ... Giraldez AJ, Mishima Y, Rihel J, Grocock RJ, Van Dongen S, Inoue K, Enright AJ, Schier AF (April 2006). "Zebrafish MiR-430 ... Thatcher EJ, Bond J, Paydar I, Patton JG (May 2008). "Genomic organization of zebrafish microRNAs". BMC Genomics. 9: 253. doi: ... Schier AF, Giraldez AJ (2006). "MicroRNA function and mechanism: insights from zebra fish". Cold Spring Harbor Symposia on ...
The enzyme is implicated in the trafficking and signaling of type I bone morphogenetic protein (BMP) receptors in zebra fish ( ... The gene encodes SPTLC1 protein, which together with SPTLC2 protein, forms serine palmitoyltransferase (SPT) in humans. SPT is ... The gene encodes SPTLC2 protein which is one of two subunits of SPT. As mutations in the gene affect the same enzyme as those ... The protein also has de novo DNA methyltransferase activity which is responsible for establishing methylation patterns during ...
"Suppression and overexpression of adenosylhomocysteine hydrolase-like protein 1 (AHCYL1) influences zebrafish embryo ... 2006). "IRBIT, an inositol 1,4,5-trisphosphate receptor-binding protein, specifically binds to and activates pancreas-type Na+/ ... receptor-binding protein, is released from the IP3 receptor upon IP3 binding to the receptor". J Biol Chem. 278 (12): 10602-12 ...
This particular protein contains two domains: the C terminal and N terminal domain. The N-terminal domain is larger than the C ... Kryukov GV; Gladyshev VN (December 2000). "Selenium metabolism in zebrafish: multiplicity of selenoprotein genes and expression ... Fujii M; Saijoh K; Kobayashi T; Fujii S; Lee MJ; Sumino K (October 1997). "Analysis of bovine selenoprotein P-like protein gene ... In molecular biology, the protein domain selenoprotein P (SelP) is the only known eukaryotic selenoprotein that contains ...
"The Golgi matrix protein giantin is required for normal cilia function in zebrafish". Biology Open. 6 (8): 1180-1189. doi: ... 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi: ... 2002). "Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein ... "The Golgi matrix protein giantin is required for normal cilia function in zebrafish". Biology Open. 6 (8): 1180-1189. doi: ...
... and zebrafish (AAH77859) proteins. Structurally, there are twelve transmembrane helices with the N- and C- termini directed to ... PCFT is located on chromosome 17q11.2 and consists of five exons encoding a protein with 459 amino acids and a MW of ~50kDa. ... The proton-coupled folate transporter is a protein that in humans is encoded by the SLC46A1 gene. The major physiological roles ... Sharma S, Dimasi D, Bröer S, Kumar R, Della NG (April 2007). "Heme carrier protein 1 (HCP1) expression and functional analysis ...
Alfa-2 (α2) adrenergički receptor (ili adrenoceptor) je G protein-spregnuti receptor koji vezuje Gi heterotrimerni G protein. ... of duplicated fourth alpha2-adrenergic receptor subtype by cloning and mapping of five receptor genes in zebrafish". Molecular ...
Required for many proteins and enzymes, notably hemoglobin to prevent anemia Meat, seafood, nuts, beans, dark chocolate[23] ... zebrafish) and Xenopus laevis (African clawed frog).[39][48] In animals, supplemental boron has been shown to reduce calcium ...
Jacob F; Monod J (June 1961). "Genetic regulatory mechanisms in the synthesis of proteins". J Mol Biol. 3 (3): 318-56. doi: ... Amsterdam, A; Hopkins, N (September 2006). "Mutagenesis strategies in zebrafish for identifying genes involved in development ... Wu, DD; Irwin, DM; Zhang, YP (November 2011). "De novo origin of human protein-coding genes.". PLOS Genetics 7 (11): e1002379. ... Hershey, AD; Chase, M (1952). "Independent functions of viral protein and nucleic acid in growth of bacteriophage". The Journal ...
Fractionation of proteins by heparin chromatography։ Methods Mol Biol։ Methods in Molecular Biology™ 424։ 2008։ էջեր 213-21։ ... Structural characterization of glycosaminoglycans from zebrafish in different ages»։ Glycoconjugate Journal 26 (2): 211-218։ ... Cell surface heparin sulfate is a receptor for attachment of envelope protein-free retrovirus-like particles and VSV-G ... Coombe D.R., Kett W.C. (2005)։ «Heparan sulfate-protein interactions: therapeutic potential through structure-function insights ...
There are a number of ALS genes that encode for RNA-binding proteins. The first to be discovered was TDP-43 protein,[35] a ... The zebrafish has transparent embryos that can be injected with DNA or RNA and has a lifespan of up to two years.[79] Induced ... Mutant SOD1 protein forms intracellular aggregations that inhibit protein degradation. Cytoplasmic aggregations of wild-type ( ... Once these mutant RNA-binding proteins are misfolded and aggregated, they may be able to misfold normal protein both within and ...
October 2017). "Multiplexed quantification of proteins and transcripts in single cells". Nature Biotechnology. 35 (10): 936-939 ... The first vertebrate animals to be mapped in this way were Zebrafish[80][81][82] and Xenopus laevis.[83] In each case multiple ... In 2017, two approaches were introduced to simultaneously measure single-cell mRNA and protein expression through ... "Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo". Science. 360 (6392): 981-987. Bibcode ...
"Design and Selection of Novel Cys2His2 Zinc Finger Proteins". Annu. Rev. Biochem. 70: 313-40. PMID 11395410. doi:10.1146/ ... "Targeted gene inactivation in zebrafish using engineered zinc finger nucleases". Nat. Biotechnol. 26 (6): 695-701. PMC 2502069 ... "Heritable Targeted Gene Disruption in Zebrafish Using Designed Zinc Finger Nucleases". Nat. Biotechnol. 26 (6): 702-8. PMC ...
Secondary databases: UniProt, database of protein sequences grouping together Swiss-Prot, TrEMBL and Protein Information ... Arabidopsis Information Resource and Zebrafish Information Network ... Other databases: Protein Data Bank, Ensembl and InterPro. *Specialised genomic databases: BOLD, Saccharomyces Genome Database, ... This includes a number of model organism databases and multi-species protein databases, software development groups, and a ...
It has been suggested that the evolution of the tetrapod limb from lobe-finned fishes is related to the loss of these proteins. ... Begemann, Gerrit (2009). "Evolutionary Developmental Biology". Zebrafish. 6 (3): 303-4. doi:10.1089/zeb.2009.0593.. ... The genetic basis for the formation of the fin rays is thought to be genes coded for the production of certain proteins. ... Gatesy, J. (1 May 1997). "More DNA support for a Cetacea/Hippopotamidae clade: the blood-clotting protein gene gamma-fibrinogen ...
Secondary databases: UniProt, database of protein sequences grouping together Swiss-Prot, TrEMBL and Protein Information ... Arabidopsis Information Resource and Zebrafish Information Network ... Other databases: Protein Data Bank, Ensembl and InterPro. *Specialised genomic databases: BOLD, Saccharomyces Genome Database, ...
These regions mediate protein-protein interactions and help DVL channel signals into either the β-catenin or the β-catenin ... There are vertebrae homologs in zebrafish, Xenopus (Xdsh), mice (Dvl1, -2, -3) and humans (DVL-1, -2, -3). Dsh relays complex ... This region mediates many protein-protein interactions and regulates multiple biological processes.[3] ... This protein-related article is a stub. You can help Wikipedia by expanding it.. *v ...
miR-181 expression has a reverse correlation with Tcl1 protein expression. mir-181 a and b are over-expressed and act as bad ... miR-181 which has been predicted or experimentally confirmed in a wide number of vertebrate species as rat, zebrafish, and in ... It has been shown that miR-181 targets the homeobox protein Hox-A11 and participates in establishing muscle tissue ... They target and modulate protein expression by inhibiting translation and / or inducing degradation of target messenger RNAs. ...
Zebrafish[21] and prairie voles[22] have also become more common, especially in the social and behavioral scopes of ... There are many genes and proteins that contribute to the formation and development of the central nervous system, many of which ... One area deals with molecular processes and the function of certain proteins, often in conjunction with cell signaling and ... Oliveira RF, Silva JF, Simões JM (June 2011). "Fighting zebrafish: characterization of aggressive behavior and winner-loser ...
"High-sensitivity real-time analysis of nanoparticle toxicity in green fluorescent protein-expressing zebrafish". Small. ... Wikimedia Commons has media related to Green fluorescent proteins.. *A comprehensive article on fluorescent proteins at ... The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green ... There are many GFP-like proteins that, despite being in the same protein family as GFP, are not directly derived from Aequorea ...
Such modifications are often guided by protein X-ray crystallography of the protein-fragment complex.[41][42][43] The ... zebrafish, worms, immortalized cell lines, primary cell lines, patient-derived cell lines and whole animal models. These ... The majority of targets selected for drug discovery efforts are proteins, such as G-protein-coupled receptors (GPCRs) and ... They induce apoptosis [63][64] and protein cascade via proteinase inhibitor,[63] have defense functions,[65] and regulate plant ...
Zhu X, Sen J, Stevens L, Goltz JS, Stein D (Sep 2005). "Drosophila pipe protein activity in the ovary and the embryonic ... on learning and memory in zebrafish". Psychopharmacology. 231 (9): 1975-85. doi:10.1007/s00213-013-3340-1. PMID 24311357. Stone ... "1aqy Summary". Protein Data Bank in Europe Bringing Structure to Biology. The European Bioinformatics Institute. Retrieved 11 ... A prominent kinase is cyclin-dependent kinase (or CDK), which comprises a sub-family of protein kinases. As their name implies ...
Computer modeling is an example of alternative testing methods; using computer models of chemicals and proteins, structure- ... and Zebrafish, which allow for the study of toxicology in a lower order vertebrate in vivo.[20][21] As of 2014, such animal ... activity relationships can be determined, and chemical structures that are likely to bind to, and interfere with, proteins with ... "High-Throughput Video Processing of Heart Rate Responses in Multiple Wild-type Embryonic Zebrafish per Imaging Field" ...
"mc1r Pathway regulation of zebrafish melanosome dispersion". Zebrafish. 5 (4): 289-95. 2008. doi:10.1089/zeb.2008.0541. PMID ... "Bacterial membrane proteins". Microbiological sciences. 4 (4): 100-5. 1987. PMID 3153178.. Unknown parameter ,last૧=. ignored ( ... "Behavioral genetic approaches to visual system development and function in zebrafish" (PDF). J Neurobiol. 54 (1): 148-60. 2003 ... "Regulation of pigmentation in zebrafish melanophores". Pigment Cell Res. 19 (3): 206-13. 2006. doi:10.1111/j.1600-0749.2006. ...
For example, a protein found in pigs can cause humans to reject their transplanted organs. This protein can be replaced by a ... March 2013). "Efficient genome editing in zebrafish using a CRISPR-Cas system". Nat. Biotechnol. 31 (3): 227-9. doi:10.1038/nbt ... This means that a specific DNA sequence will code for the same protein in all organisms. Due to this similarity in protein ... a component in milk whey protein that causes allergies. Pharming examples: Haemoglobin as a blood substitute Human protein C ...
CD59 - povrchová molekula SC radená do skupiny proteínov regulujúcich komplement (Complement Regulatory Proteins -CRP)[10] ... Thyroid Hormone Stimulates the Proliferation of Sertoli Cells and Single Type A Spermatogonia in Adult Zebrafish (Danio rerio) ... transferin - protein z krvnej plazmy potrebuný pre prenos železa do bunky[3]. ...
Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... Neurotrophin-6 and neurotrophin-7 also exist but are only found in zebrafish.[8] ... These two proteins are p75 (NTR), which binds to all neurotrophins, and subtypes of Trk, which are each specific for different ... It is a protein growth factor that has activity on certain neurons of the peripheral and central nervous system; it helps to ...
Functional genomics focuses on the dynamic aspects such as gene transcription, translation, and protein-protein interactions, ... The zebrafish Brachydanio rerio is used for many developmental studies on the molecular level, and the plant Arabidopsis ... the gene for Bacteriophage MS2 coat protein.[13] Fiers' group expanded on their MS2 coat protein work, determining the complete ... "Protein Science. 9 (1): 197-200. doi:10.1110/ps.9.1.197. PMC 2144435 . PMID 10739263.. ...
It has been suggested that the evolution of the tetrapod limb from lobe-finned fishes is related to the loss of these proteins. ... Begemann, Gerrit (2009). "Evolutionary Developmental Biology". Zebrafish. 6 (3): 303-4. doi:10.1089/zeb.2009.0593.. ... filaments of elastic protein resembling the horny keratin in hair and feathers.[16] Originally the pectoral and pelvic girdles ... "Loss of fish actinotrichia proteins and the fin-to-limb transition". Nature. 466 (7303): 234-237. Bibcode:2010Natur.466..234Z. ...
... protein = , water = , alcohol = , caffeine = , vitA_ug = , vitA_iu = , betacarotene_ug = , lutein_ug = , thiamin_mg = , ...
Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat Biotechnol. 2013. PMID 23360964. doi:10.1038/nbt.2501.. ... "記憶"[5]。全名為常間回文重複序列叢集/常間回文重複序列叢集關聯蛋白系統(Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins
2005). "SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans". Science (New York, New York.). 310 ... Donatien PD, Orlow SJ (1995). "Interaction of melanosomal proteins with melanin". European journal of biochemistry / FEBS. 232 ...
Secondary databases: UniProt, database of protein sequences grouping together Swiss-Prot, TrEMBL and Protein Information ... The Zebrafish Information Network (ZFIN) is an online biological database of information about the zebrafish (Danio rerio). The ... Vega Zebrafish Genome Annotation. *FishMap : The Zebrafish Community Genomics Browser maintained at the Institute of Genomics ... Sprague et al. (2008). The Zebrafish Information Network: the zebrafish model organism database provides expanded support for ...
Effects on proteins[edit]. In mammals, 20% to 40% of proteins contain repeating sequences of amino acids encoded by short ... An archaic form of splicing preserved in Zebrafish is known to use microsatellite sequences within intronic mRNA for the ... 1998). "A census of protein repeats". J. Mol. Biol. 293 (1): 151-160. doi:10.1006/jmbi.1999.3136. PMID 10512723.. ... Mattick J. S. (2003). "Challenging the dogma: the hidden layer of non-protein-coding RNAs in complex organisms". BioEssays. 25 ...
This results in the lowered expression of Reelin protein, essential for proper cortex lamination and cerebellum development. ... then nutritive requirements of rats were used by Thomas Burr Osborne and Lafayette Mendel to determine the details of protein ...
Secondary databases: UniProt, database of protein sequences grouping together Swiss-Prot, TrEMBL and Protein Information ... Arabidopsis Information Resource and Zebrafish Information Network ... Other databases: Protein Data Bank, Ensembl and InterPro. *Specialised genomic databases: BOLD, Saccharomyces Genome Database, ... Sequence alignment shows the relations between genes or between proteins, leading to a better understanding of their homology ...
We have generated several hundred lines of zebrafish (Danio rerio), each heterozygous for a recessive embryonic lethal mutation ... Many ribosomal protein genes are cancer genes in zebrafish PLoS Biol. 2004 May;2(5):E139. doi: 10.1371/journal.pbio.0020139. ... Surprisingly, 11 of the 12 lines were each heterozygous for a mutation in a different ribosomal protein (RP) gene, while one ... We have generated several hundred lines of zebrafish (Danio rerio), each heterozygous for a recessive embryonic lethal mutation ...
Nonequivalence of Mouse and Zebrafish Hoxa3 Proteins.. Our data show that the mouse and zebrafish Hoxa3 proteins have ... 1 B-F). Analysis of the zebrafish hoxa3a protein using the HA tag showed that the protein was present and had the correct ... Conserved Hoxa3 Protein Functions.. We tested whether zebrafish hoxa3a protein was able to substitute for mouse Hoxa3 in mice ... Although protein sequence alignment showed that overall, the zebrafish hoxa3a and mouse Hoxa3 and Hoxd3 proteins showed similar ...
... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... PROSITE; a protein domain and family database. More...PROSITEi. View protein in PROSITE. PS01122, CASPASE_CYS, 1 hit. PS50207 ... Integrated resource of protein families, domains and functional sites. More...InterProi. View protein in InterPro. IPR029030, ... Simple Modular Architecture Research Tool; a protein domain database. More...SMARTi. View protein in SMART. SM00115, CASc, 1 ...
As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 7955.ENSDARP00000112143 ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes ...
By determination of the protein content in the zebrafish skeletal ECM with major changes in protein abundance during ... In this thesis we provide a first insight in the extracellular protein content of the zebrafish skeleton, identify a role for ... Carboxypeptidase Z an extracellular protein in zebrafish development. Historically the skeleton, in particular bone, was ... After the identification of potential regulatory proteins in the developing zebrafish skeleton during the MS-based approach, ...
... tropical zebrafish can repair a severed spine in eight weeks. Researchers found a special grow … ... Healing protein at spinal cord injured zebrafish Back to overview Mammals cannot regenerate their complex nervous tissue but ... We (humans) share 70 per cent of our genetic code with zebrafish, and the human CTGF protein is nearly 90% similar to the one ... CTGF is a large protein, made of four smaller parts, and it has more than one function. But the second half of the CTGF protein ...
General protein information Go to the top of the page Help Preferred Names. serine/threonine-protein phosphatase 2A catalytic ... ppp2cab protein phosphatase 2 catalytic subunit alpha b [ Danio rerio (zebrafish) ] Gene ID: 393885, updated on 22-Nov-2020 ... mRNA and Protein(s) * XM_021468779.1 → XP_021324454.1 serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform ... mRNA and Protein(s) * NM_200911.3 → NP_957205.2 serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform ...
Using this system, we have investigated the role of cAMP protein kinase A (PKA) signaling in pigment cell differentiation. ... In zebrafish (Danio rerio), iridophores are specified from neural crest cells and represent a tractable system for examining ... Protein Kinase A Signaling Inhibits Iridophore Differentiation in Zebrafish by Cynthia D. Cooper ... Protein Kinase A Signaling Inhibits Iridophore Differentiation in Zebrafish. Journal of Developmental Biology. 2018; 6(4):23. ...
... we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish ... Single-cell Photoconversion in Living Intact Zebrafish…. Published 3/19/2018. Tracking Cells in GFP-transgenic Zebrafish Using ... Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)… ... Cell Tracking Using Photoconvertible Proteins During Zebrafish Development. Verónica A. Lombardo1, Anje Sporbert1, Salim ...
... zebrafish). Find diseases associated with this biological target and compounds tested against it in bioassay experiments. ... Protein target information for Calcium-transporting ATPase ( ...
It is a very complex process that requires the coordinated actions of ribosomal and non-ribosomal proteins. Impairment of ... the protein-synthesizing machines, is fundamental in all cells. ...
Cheuk Wun Li and Wei Ge "Regulation of the Activin-Inhibin-Follistatin System by Bone Morphogenetic Proteins in the Zebrafish ... In the present study, we developed a novel coculture system to co-incubate zebrafish bone morphogenetic protein 2b or 4 ( ... Cheuk Wun Li, Wei Ge "Regulation of the Activin-Inhibin-Follistatin System by Bone Morphogenetic Proteins in the Zebrafish ... We have previously identified zebrafish bone morphogenetic proteins (BMPs) as oocyte factors that may act on follicle cells; ...
Zebrafish Proteasomal Ubiquitin-Dependent Protein Catabolic Process. Polyclonal Antibody - Phospho-GSK-3α/β (Ser21/9) Antibody ... Rat Scf-Dependent Proteasomal Ubiquitin-Dependent Protein Catabolic Process * Human Proteasomal Ubiquitin-Dependent Protein ... Monkey Regulation of Proteasomal Ubiquitin-Dependent Protein Catabolic Process * Mouse Proteasomal Ubiquitin-Dependent Protein ... Hamster Proteasomal Ubiquitin-Dependent Protein Catabolic Process * Dog Proteasomal Ubiquitin-Dependent Protein Catabolic ...
... zebrafish). Find diseases associated with this biological target and compounds tested against it in bioassay experiments. ... Protein target information for PCNA-associated factor ( ...
Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 7955.ENSDARP00000121318 ... Protein predictedi ,p>This indicates the type of evidence that supports the existence of the protein. Note that the protein ... to allow unambiguous identification of a protein.,p>,a href=/help/protein_names target=_top>More...,/a>,/p>Protein namesi. ... Pfam protein domain database. More...Pfami. View protein in Pfam. PF00620 RhoGAP, 1 hit. PF07647 SAM_2, 1 hit. PF01852 START ...
The forkhead protein Foxj1 specifies node-like cilia in Xenopus and zebrafish embryos.. Stubbs JL1, Oishi I, Izpisúa Belmonte ... The Forkhead protein, FoxJ1, specifies node-like cilia in Xenopus and Zebrafish embryos ... The Forkhead protein, FoxJ1, specifies node-like cilia in Xenopus and Zebrafish embryos ... The Forkhead protein, FoxJ1, specifies node-like cilia in Xenopus and Zebrafish embryos ...
... which allow conditional over-expression of zic2a in zebrafish embryos. These transgenic, zic2a-expressing zebrafish will be ... Zic genes encode proteins that contain zinc fingers used to bind DNA and modify transcription of target genes. Several zic ... The goal of this study was to begin determining the regulatory nature of zic2a in the zebrafish (Danio rerio) central nervous ... These constructs were further modified and then used to develop three stable transgenic zebrafish lines, ...
In in vitro assays, the secreted protein Sonic hedgehog mimics the inducing effects of notochord on both presomitic mesoderm ... Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish Nature. 1996 Aug 1;382(6590):452-5. doi: ... suggesting that both patterning activities of the notochord may be mediated by this protein in vivo. In zebrafish, however, ... In in vitro assays, the secreted protein Sonic hedgehog mimics the inducing effects of notochord on both presomitic mesoderm ...
Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate ... The Handbook of Analysis and Purification of Peptides and Proteins by Reversed-Phase HPLC, GraceVydac, Deerfield, Ill, USA, 3rd ... W. Zheng, Z. Li, A. T. Nguyen, C. Li, A. Emelyanov, and Z. Gong, "Xmrk, Kras and Myc transgenic zebrafish liver cancer models ... Y. Jin, X. Zhang, D. Lu, and Z. Fu, "Proteomic analysis of hepatic tissue in adult female Zebrafish (Danio rerio) exposed to ...
... and we took advantage of the fact that nuclear localization of zebrafish Meis proteins is mediated by Pbx proteins (Vlachakis ... Meis family proteins are required for hindbrain development in the zebrafish Message Subject (Your Name) has sent you a message ... Meis family proteins are required for hindbrain development in the zebrafish. Seong-Kyu Choe, Nikolaos Vlachakis, Charles G. ... Meis family proteins are required for hindbrain development in the zebrafish. Seong-Kyu Choe, Nikolaos Vlachakis, Charles G. ...
Citation: Elusive link between seizures, cell signaling protein IDd in zebrafish (2021, January 11) retrieved 21 June 2021 ... Elusive link between seizures, cell signaling protein IDd in zebrafish. by Virginia Tech ... So not only is the protein affecting hyperactivity, were also having some effect on the protein itself," Brunal said. "I think ... By comparing normal, wild-type zebrafish and mutant zebrafish with connexin 36 deficiencies, they found that connexin 36 ...
... have described the key role that GLUT2 protein plays in embryonic brain development in zebrafish. A new article -highlighted on ... Research group identifies GLUT2 proteins role in zebrafish brain development. by University of Barcelona ... Citation: Research group identifies GLUT2 proteins role in zebrafish brain development (2015, January 14) retrieved 16 April ... have described the key role that GLUT2 protein plays in embryonic brain development in zebrafish. A new article -highlighted on ...
... differential and complementary expression patterns of the zebrafish crabp1a and crabp1b genes imply that subfunctionalization ... may be the mechanism for the retention of both crabp1 duplicated genes in the zebrafish genome; GenBank BI533516 ... cellular retinoic acid-binding protein 1, zebrafish; CRABP1 protein, zebrafish; crabp1a protein, zebrafish; crabp1b protein, ... Fish Proteins: 10*Zebrafish Proteins: 1*zebrafish cellular retinoic acid-binding protein 1 ...
The transmembrane protein Crb2a regulates cardiomyocyte apicobasal polarity and adhesion in zebrafish ... The transmembrane protein Crb2a regulates cardiomyocyte apicobasal polarity and adhesion in zebrafish ... 2012). Crb apical polarity proteins maintain zebrafish retinal cone mosaics via intercellular binding of their extracellular ... The transmembrane protein Crb2a regulates cardiomyocyte apicobasal polarity and adhesion in zebrafish ...
Zebrafish BMP-2a protein (111-BM/CF) is manufactured by R&D Systems, over 97% purity. Reproducible results in bioactivity ... as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, ... Reviews for Recombinant Zebrafish BMP-2a Protein, CF. There are currently no reviews for this product. Be the first to review ... Recombinant Zebrafish BMP-2a Protein, CF Summary. Purity. >97%, by SDS-PAGE under reducing conditions and visualized by silver ...
Zebrafish BMP-4 protein (1128-BM/CF) is manufactured by R&D Systems, over 97% purity. Reproducible results in bioactivity ... as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, ... Similarly to other TGF-beta superfamily proteins, BMPs are highly conserved across animal species. Zebrafish BMP-4 protein ... Reviews for Recombinant Zebrafish BMP-4 Protein, CF. There are currently no reviews for this product. Be the first to review ...
S100 The bone morphogenetic protein type II receptor is critical for venous angiogenesis in zebrafish ... S100 The bone morphogenetic protein type II receptor is critical for venous angiogenesis in zebrafish ... This phenotype was reproduced in BMPR2 dominant-negative zebrafish and following knockdown of the zebrafish homologues of BMPR2 ... Here we use zebrafish, which have a well-documented pattern of angiogenesis, as a model organism to address this question. ...
Zebrafish Pnpla6 protein shares 73% identity with human PNPLA6 and is highly conserved compared with Pnpla6 protein in other ... Knockdown of Pnpla6 protein results in motor neuron defects in zebrafish. Yang Song, Molin Wang, Fei Mao, Ming Shao, Baochang ... Knockdown of Pnpla6 protein results in motor neuron defects in zebrafish. Yang Song, Molin Wang, Fei Mao, Ming Shao, Baochang ... Knockdown of Pnpla6 protein results in motor neuron defects in zebrafish. Yang Song, Molin Wang, Fei Mao, Ming Shao, Baochang ...
This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by ... Protein localization to the diverse cellular compartments is conserved between zebrafish and human, allowing investigation of ... Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina. José M. Mateos1, Gery ... We present a method to investigate the subcellular protein localization in the larval zebrafish retina by combining super- ...
Zebrafish Protein Complex Zebrafish Protein Complex: Monoclonal Antibody - Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb, ... Zebrafish Positive Regulation of Protein Amino Acid Phosphorylation Zebrafish Positive Regulation of Protein Amino Acid ... Zebrafish Protein Stabilization Zebrafish Protein Stabilization: Monoclonal Antibody - p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb ... Zebrafish Protein Amino Acid Phosphorylation Zebrafish Protein Amino Acid Phosphorylation: Polyclonal Antibody - p44/42 MAPK ( ...
  • By exposing zebrafish embryos to the thyroid hormone triiodothyronine (T3) we showed that exposure accelerates ossification of craniofacial elements including the opercle and ceratohyal in a dose-dependent manner. (
  • The forkhead protein Foxj1 specifies node-like cilia in Xenopus and zebrafish embryos. (
  • Cilia length in the KV of Zebrafish embryos (n=20 cilia from each of 6 embryos) injected with the ZFoxJ1-MO (atg) or with the control-MO (control). (
  • These constructs were further modified and then used to develop three stable transgenic zebrafish lines, which allow conditional over-expression of zic2a in zebrafish embryos. (
  • In in vitro assays, the secreted protein Sonic hedgehog mimics the inducing effects of notochord on both presomitic mesoderm and neural plate explants of amniote embryos, suggesting that both patterning activities of the notochord may be mediated by this protein in vivo. (
  • In order to investigate GLUT2 function in embryonic brain development , the UB group knocked down GLUT2 in zebrafish embryos and examine the anomalies that took place. (
  • Zebrafish embryos without GLUT2 present features which are similar to the ones that characterise this syndrome. (
  • So zebrafish embryos provide a unique opportunity to unravel the mechanisms following this rare disease. (
  • The mRNA transcripts in GFP+ve cells were determined using massively parallel (Illumina) sequencing, mapped to the Zv8 zebrafish genome and differences in transcript abundance between LDN193189 and DMSO treated embryos were determined using cufflinks. (
  • Results Inhibition of BMP signalling with LDN193189 in zebrafish embryos after dorso-ventral patterning has occurred blocked venous but not arterial angiogenesis. (
  • Marker gene analysis showed that rnf141 may play a role in normal dorsoventral patterning of zebrafish embryos, suggesting that rnf141 may have a broad function during early development of vertebrates. (
  • The G863V mutation reduces the production and size of ApoB-containing lipoproteins in zebrafish embryos and results in the accumulation of cytoplasmic lipid droplets in the yolk syncytial layer. (
  • We here report the detection of zebrafish papp-a2 mRNA in chordamesoderm, notochord, and lower jaw of zebrafish ( Danio rerio ) embryos, and that papp-a2 knockdown embryos display broadened axial mesoderm, notochord bends, and severely reduced cranial cartilages. (
  • Based on these results, we conclude that Papp-a2 modulates Bmp and Notch signaling by independent mechanisms in zebrafish embryos. (
  • Translational suppression or CRISPR/Cas9 genome editing of apol1 in zebrafish embryos results in podocyte loss and glomerular filtration defects. (
  • Therefore, both AFP4 proteins are essential for gastrulation of zebrafish embryos. (
  • Morpholino knock down of the kinases Fyn and Yes, induced characteristic phenotypes in 1 day old zebrafish embryos and the lysates induced distinct changes in the PTK activity profiles compared to wild-type lysates. (
  • A quantitative proteomics approach, using stable isotope di-methyl labeling was used to compare protein phosphorylation between wild type and Fyn/Yes knockdown embryos that display convergence and extension cell movement defects. (
  • In this thesis in vivo phosphorylation was studied at different stages and under different experimental conditions in developing zebrafish embryos. (
  • Results showed depletion in numbers and disordering migration of PGCs in human or zebrafish DYRK1A overexpressed zebrafish embryos. (
  • Quantitative proteome analysis indicated that embryonic proteins were significantly altered in DYRK1A overexpressed embryos. (
  • To investigate motor function, the researchers studied coiling behavior in zebrafish embryos. (
  • We used morpholinos to knock down protein expression in the zebrafish embryos and subsequently, we determined the effects on the PTK activity profiles. (
  • Basically, this method involves ectopic overexpression of epitope-tagged proteins via mRNA injections in one-to-four-cell stage embryos and subsequent protein detection after various time points. (
  • The results of developmental effect experiment showed that captan can significantly decrease the heartbeats and inhibit the hatching rate and growth of zebrafish embryos. (
  • Moreover, captan exposure can induce a series of deformities, including pericardial edema, yolk sac edema, spine curvature, and tail bending, in zebrafish embryos during the developmental period. (
  • The study consists in a co-clinical trial by using zebrafish embryos. (
  • By specifically expressing the SPoT cassette in neurons of transgenic zebrafish embryos, we reveal sites of neuronal protein synthesis in diverse cellular compartments during early development. (
  • These phenotypical characteristics can be mimicked in wild-type embryos by acetazolamide treatment, suggesting that CA5 activity in zebrafish is essential for a proper development. (
  • Islet-3 is an LIM/homeodomain protein that is expressed specifically in the eyes and the presumptive tectum in the central nervous system of zebrafish (Danio rerio) embryos. (
  • Using techniques to globally repress or activate Hedgehog signaling in zebrafish embryos followed by microarray-based expression profiling, we have discovered a cohort of genes whose expression responds significantly to loss or gain of Hedgehog function. (
  • Proteins of embryos 72 and 120 h postfertilization (hpf) were isolated and analyzed both by two-dimensional (2D) LC followed by ESI-MS/MS and by 2D PAGE followed by MALDI-TOF/TOF protein identification. (
  • Co-overexpression of MafT and Nrf2 synergistically activated MARE-mediated gene expression in zebrafish embryos. (
  • The zebrafish homologue of the human myeloperoxidase (MPO) gene, which is specific to cells of the neutrophil lineage, was cloned and used to synthesize antisense RNA probes for in situ hybridization analyses of zebrafish embryos. (
  • Zhong Y, Lu L, Zhou J, Li Y, Liu Y, Clemmons DR, Duan C. IGF binding protein 3 exerts its ligand-independent action by antagonizing BMP in zebrafish embryos . (
  • In his research project, Fabrice Laroche studied responses to chitin in zebrafish embryos, and he described chitin signalling pathways. (
  • Sub-lethal effects on the proteome of zebrafish embryos were analysed for tralopyril and TPBP. (
  • Since the pathway has never been investigated during embryogenesis, we characterized the first three steps of BER in zebrafish extracts from unfertilized eggs, embryos at different developmental stages and adults. (
  • In fact, eggs and early stage embryos lack DNA polymerase-β protein. (
  • Previously we showed that when zebrafish AP endonuclease protein (ZAP1) level is knocked down, embryos cease dividing after the initial phase of rapid proliferation and die without apoptosis shortly thereafter. (
  • In an effort to understand the role of AP endonuclease 1 in early development, we recently explored the effects of knocking down expression of the gene in zebrafish embryos ( 16 ). (
  • Although zebrafish zygotic transcription begins at the MBT, failure to initiate zygotic transcription is unlikely to be the cause of death, because mouse embryos initiate zygotic transcription at the two-cell stage but Apex -/- mouse embryos do not fail until E7-E9. (
  • We have generated several hundred lines of zebrafish (Danio rerio), each heterozygous for a recessive embryonic lethal mutation. (
  • In zebrafish ( Danio rerio ), iridophores are specified from neural crest cells and represent a tractable system for examining mechanisms of cell fate and differentiation. (
  • The goal of this study was to begin determining the regulatory nature of zic2a in the zebrafish (Danio rerio) central nervous system. (
  • Y. Jin, X. Zhang, D. Lu, and Z. Fu, "Proteomic analysis of hepatic tissue in adult female Zebrafish ( Danio rerio ) exposed to atrazine," Archives of Environmental Contamination and Toxicology , vol. 62, no. 1, pp. 127-134, 2012. (
  • P. Carlson, D. M. Smalley, and R. J. van Beneden, "Proteomic analysis of arsenic-exposed zebrafish ( Danio rerio ) identifies altered expression in proteins involved in fibrosis and lipid uptake in a gender-specific manner," Toxicological Sciences , vol. 134, no. 1, pp. 83-91, 2013. (
  • The study points the use of zebrafish (Danio rerio) as a model to study diseases produced by GLUT2 alterations, such as the Fanconi-Bickel syndrome (FBS), a rare glycogen storage disease characterized by the absence of GLUT2 which causes severe liver and kidney dysfunction. (
  • Here, we describe the generation of a vertebrate model of PNPLA6 insufficiency using morpholino oligonucleotide knockdown in zebrafish ( Danio rerio ). (
  • Two genes, namely afp4a and afp4b coding for AFP4s, were identified in gibel carp ( Carassius auratus gibelio ) and zebrafish ( Danio rerio ). (
  • For example, the lipid pregnenolone, produced from cholesterol by Cyp11a1 enzyme, was shown to promote zebrafish ( Danio rerio , Dr ) embryonic cell movements by increasing yolk cytoplasmic layer (YCL) microtubule abundance [ 6 ]. (
  • We use Danio rerio , or Zebrafish, as a model organism to study the role of Crk and Crkl in the developing retina. (
  • Toxicity effects of captan on different life stages of zebrafish (Danio rerio). (
  • The objective of this study was to evaluate the toxicity and developmental effects of captan on different life stages (embryo and adult) of zebrafish (Danio rerio). (
  • Developmental toxicity of kresoxim-methyl during zebrafish (Danio rerio) larval development. (
  • The case for thyroid disruption in early life stage exposures to thiram in zebrafish (Danio rerio). (
  • Imidacloprid induces adverse effects on fish early life stages that are more severe in Japanese medaka (Oryzias latipes) than in zebrafish (Danio rerio). (
  • The model organism zebrafish ( Danio rerio ) is particularly amenable to studies deciphering regulatory genetic networks in vertebrate development, biology, and pharmacology. (
  • The zebrafish ( Danio rerio ) is an attractive vertebrate model organism for studies into the molecular mechanisms of development ( 2 ), pathology ( 3 ), and pharmacology ( 4 , 5 ). (
  • Genome-wide chemical mutagenesis screens in the zebrafish (Danio rerio) have led to the identification of novel genes affecting vertebrate erythropoiesis. (
  • 2 3 Many of the genes known to regulate erythropoiesis in mammals have been identified in another vertebrate animal model, the zebrafish (Danio rerio) . (
  • In zebrafish ( Danio rerio ), O 2 consumption rate ( Ṁ O 2 ) has been reported to approximately double after feeding ( Lucas and Priede, 1992 ), but the simultaneous changes in carbon dioxide excretion rate ( Ṁ CO 2 ) and nitrogenous waste excretion rate ( Ṁ N =ammonia-N+urea-N) have not been studied. (
  • The zebrafish, Danio rerio , is an established genetic and developmental model used in genetic screens to uncover genes necessary for early development. (
  • Mice and other mammals have 39 Hox genes arranged in four clusters located on four different chromosomes, whereas zebrafish and other teleosts have 48 Hox genes in seven clusters resulting from a genome-wide duplication ( 2 ). (
  • Hox genes from the same group (transparalogous genes or paralogues) arose from duplication, and share more similarity in protein sequence and expression pattern than genes within a cluster. (
  • Scientists from the Duke University analyzed all of the zebrafish genes whose activity suddenly changed immediately following a spinal injury. (
  • Zic genes encode proteins that contain zinc fingers used to bind DNA and modify transcription of target genes. (
  • Meis homeodomain proteins function as Hox-cofactors by binding Pbx and Hox proteins to form multimeric complexes that control transcription of genes involved in development and differentiation. (
  • Mutations in either gene lead to posterior transformations of embryonic segments, without affecting the expression of Hox genes, showing that both Exd and Hth are required for Hox protein function during fly development. (
  • Principal Findings We identified two zebrafish EWSR1 orthologues, ewsr1a and ewsr1b, and demonstrate that both genes are expressed maternally, and are expressed ubiquitously throughout zebrafish embryonic development. (
  • The unfolded protein response (UPR) is a complex network of sensors and target genes that ensure efficient folding of secretory proteins in the endoplasmic reticulum (ER). (
  • By assessing the magnitude of activation of UPR sensors and target genes in the liver of zebrafish larvae exposed to three commonly used ER stressors (tunicamycin, thapsigargin and Brefeldin A), we have identified distinct combinations of UPR sensors and targets (i.e. subclasses) activated by each stressor. (
  • Collectively, we conclude that various NLS-fused Cas9 proteins and Cas9 mRNAs have similar genome editing efficiencies on targeting single or multiple genes, suggesting that the efficiency of CRISPR/Cas9 genome editing is highly dependent on guide RNAs (gRNAs) and gene loci. (
  • Phylogenetic analysis demonstrated the existence of five prkcs (the genes representing PKCs) in zebrafish, two paralogous forms of prkca and prkcb and one prkcg variant. (
  • People and zebrafish share most protein-coding genes, and CTGF is also produced by humans. (
  • The zebrafish genome contains a gene superfamily of 40 Ugt genes that can be divided into Ugt1 , Ugt2 , and Ugt5 families. (
  • Specifically, we measured mRNA levels of each of the 40 zebrafish Ugt genes in 11 adult tissues and found that they are expressed in a tissue-specific manner. (
  • I have mutated the duplicated and redundant zebrafish rbpms2 genes, rbpms2a and rbpms2b. (
  • In this study we assess the splicing of transcripts of these genes in adult zebrafish brain under hypoxic conditions. (
  • Here, we report Tol2 red fluorescent protein (RFP) transposon gene trap integrations in the zebrafish amyloid precursor protein a (appa) and amyloid precursor-like protein 2 (aplp2) genes. (
  • The transposon integrations are predicted to disrupt the appa and aplp2 genes to primarily produce secreted ectodomains of the corresponding proteins that are fused to RFP. (
  • The utility of the genome sequence increases with the quality of annotation of protein-encoding genes. (
  • While computational predictions tend to be rather imprecise, many putative zebrafish genes have human orthologs, and large regions of synteny exist between human and zebrafish chromosomes underlining the relevance of the zebrafish to the analysis of higher vertebrates ( 11 ). (
  • We identified homolog genes of MafG and MafK but not MafF in zebrafish, indicating the former two are conserved among vertebrates. (
  • The expression of zebrafish homologues was also examined in 2 other mammalian hematopoietic genes, Pu.1 , which appears to initiate a commitment step in normal mammalian myeloid development, and L-Plastin , a gene expressed by human monocytes and macrophages. (
  • The results demonstrate a high level of conservation of the spatio-temporal expression patterns of these genes between zebrafish and mammals. (
  • 13 This conservation of the fundamental mechanisms controlling erythropoiesis between mammals and zebrafish 4 raises the possibility that this powerful genetic system could be applied to uncover new classes of genes required in the normal development of granulocyte/macrophage cell lineages during vertebrate myelopoiesis. (
  • Current research includes genes that increase risk for autism and schizophrenia, unstudied proteins with domains of unknown function (the dark proteome) and likely neurobiological roles, and conserved alternative splicing events that are important to normal neurodevelopment. (
  • In zebrafish, this low frequency is in part explained by compensation by genes of redundant or similar function, often resulting from the additional round of teleost-specific whole genome duplication within vertebrates. (
  • We established a list of orthologous genes between human and zebrafish, which includes approximately two-thirds of human protein-coding genes. (
  • For the subset of these genes found in human MPNST CNAs, only one quarter of their orthologues were co-gained or co-lost in zebrafish, dramatically narrowing the list of candidate cancer drivers for both focal and large CNAs. (
  • However, Davis speculates that many other ribosomal protein genes will now be added to the list of candidates for other people with rare microcephaly syndromes who are searching for the molecular cause of their diseases. (
  • Although gene-targeting approaches and phenotype analysis have led to a detailed understanding of nearly 6,000 protein-coding genes, this number falls considerably short of the more than 22,000 mouse protein-coding genes. (
  • Similarly, in zebrafish genetics, one-by-one gene studies using positional cloning, insertional mutagenesis, antisense morpholino oligonucleotides, targeted re-sequencing, and zinc finger and TAL endonucleases have made substantial contributions to our understanding of the biological activity of vertebrate genes, but again the number of genes studied falls well short of the more than 26,000 zebrafish protein-coding genes. (
  • Importantly, for both mice and zebrafish, none of these strategies are particularly suited to the rapid generation of knockouts in thousands of genes and the assessment of their biological activity. (
  • So far we have identified potentially disruptive mutations in more than 38% of all known zebrafish protein-coding genes. (
  • Addressing these matters, Fabrice Laroche identified zebrafish and human lysin motif-encoding genes and studied their roles - at the cellular level and during zebrafish development. (
  • With this new knowledge of the molecular identity of BefA, the researchers could now search all other known bacterial genomes for genes encoding related proteins. (
  • One of these genes is CacyBP/SIP encoding calcyclin-binding protein (CacyBP/SIP), which is 2-fold overexpressed in the striatum of YAC128 mice, a model of HD. (
  • citation needed] Pole plasm is organized by and contains the proteins and mRNA of the posterior group genes (such as oskar, nanos gene, Tudor, vasa, and Valois). (
  • For instance, the fruit fly (D. melanogaster) has 13 PGRP genes, whose transcripts are alternatively spliced into 19 proteins, while the mosquito (Anopheles gambiae) has 7 PGRP genes, with 9 splice variants. (
  • We found that the zebrafish genome encodes two cralbp paralogs: cralbp a and cralbp b. (
  • The zebrafish genome harbors two orthologues of Cralbp, which we denoted as cralbp-a and -b . (
  • Zebrafish genetics has been facilitated substantially by the increasing availability of genome sequence information (Sanger Institute's D. rerio sequencing project) as assembly of the 1.7-Gb genome sequence nears completion. (
  • Genome annotations are supported by alignments of experimentally documented transcript or protein sequences specific for the zebrafish genome, by alignments of homologous transcript or protein sequences, and ab initio by computational gene prediction ( 10 ). (
  • As model organism-based research shifts from forward to reverse genetics approaches, largely due to the ease of genome editing technology, a low frequency of abnormal phenotypes is being observed in lines with mutations predicted to lead to deleterious effects on the encoded protein. (
  • A systematic genome-wide analysis of zebrafish protein-coding gene function. (
  • Here we describe an active project that aims to identify and phenotype the disruptive mutations in every zebrafish protein-coding gene, using a well-annotated zebrafish reference genome sequence, high-throughput sequencing and efficient chemical mutagenesis. (
  • Fortunately, she could turn back to the genome sequences of all the bacteria she had screened and ask which of these candidate proteins were shared by the beta cell expanding bacteria and absent from the inert bacteria. (
  • abstract = "The proteoglycan aggregate of the cartilage is composed of aggrecan, link protein (LP), and hyaluronan, providing resistance to compression in joints and cartilage structures. (
  • The UB research group will develop new studies to analyse the consequences that knocking out GLUT2 has on adult zebrafish . (
  • Zebrafish BMP-4 mRNA is detected throughout embryonic development and has been detected in tissues of adult fish, including the brain, heart, digestive tracts, testes, and jaw. (
  • Adult schachbrett ( sbr ) mutants exhibit delayed changes in iridophore shape and organisation caused by truncations in Tight Junction Protein 1a (ZO-1a). (
  • RT-PCR analysis in zebrafish embryo and adult tissues revealed that rnf141 transcripts are maternally derived and that rnf141 mRNA has a broad distribution. (
  • In the zebrafish retina, actively dividing retinal progenitor cells and Müller glia express mdka mRNA during development, while in the adult, mdka expression is restricted to the horizontal cells and is robustly regulated by the circadian rhythm. (
  • The purpose of this study was to determine the protein localization of Mdka in the developing and adult retinas of zebrafish. (
  • The results showed that the 96-h lethal concentration 50 (LC) values of embryo and adult zebrafish (exposed to captan) were 0.81(0.75-0.87) mg/L and 0.65(0.62-0.68) mg/L, respectively. (
  • We have characterized the proteome of adult zebrafish heart ECM. (
  • Adult zebrafish, in contrast to mammals, are able to regenerate their hearts in response to injury or experimental amputation. (
  • Here, we set out to characterize the ECM protein composition in adult zebrafish hearts, and whether it changed during the regenerative response. (
  • For this purpose, we first established a decellularization protocol of adult zebrafish ventricles that significantly enriched the yield of ECM proteins. (
  • Expression of zebrafish Papp-a, one of the two paralogs, begins during gastrulation and persists throughout the first week of development, and analyses demonstrate highly conserved patterns of expression between adult zebrafish, humans, and mice. (
  • We used respirometric theory and a new respirometry apparatus to assess, for the first time, the sequential oxidation of the major metabolic fuels during the post-prandial period (10 h) in adult zebrafish fed with commercial pellets (51% protein, 2.12% ration). (
  • To understand the genetic control of postembryonic development, we performed a dominant screen for phenotypes affecting the adult zebrafish. (
  • CONCLUSIONS: In MSNs and glial cells from YAC128 mice we did not find any changes in β‑catenin and protein ubiquitination, which were observed in the striatum of adult HD mice. (
  • In order to assess the function of Cralbp in cone visual pigment regeneration, we made use of zebrafish behavioral genetics. (
  • In determining if this approach could also be used to clarify the molecular genetics of myelopoiesis, it was found that the developmental hierarchy of myeloid precursors in the zebrafish kidney is similar to that in human bone marrow. (
  • Research in zebrafish has helped identify the cause of an unknown genetic disorder affecting a boy and two of his uncles, scientists report in an article published October 14 in the journal Genetics . (
  • The UO has been a leader in zebrafish research since the 1960s when biologist George Streisinger pioneered a new method for the study of vertebrate development and genetics with the introduction of the zebrafish as a model organism. (
  • These transgenic, zic2a-expressing zebrafish will be used in further studies to characterize the function of zic2a in the developing nervous system. (
  • Inducible and repressable oncogene-addicted hepatocellular carcinoma in Tet-on xmrk transgenic zebrafish," Journal of Hepatology , vol. 56, no. 2, pp. 419-425, 2012. (
  • Methods The transgenic Tg( fli1a:egfp ) zebrafish line, where the fli1a promoter drives GFP expression in vascular endothelial and blood cells, was used throughout this work. (
  • A variety of methods were used to dissect the role of BMP signalling in vascular development including: (i) BMP receptor inhibitors (dorsomorphin and LDN193189), (ii) antisense morpholino oligonucleotides (morpholinos) and (iii) transgenic zebrafish engineered with heat shock inducible dominant-negative BMP receptors. (
  • Lessons from transgenic zebrafish expressing the green fluorescent protein (GFP) in the myeloid lineage. (
  • We cloned the 1.5 kb promoter region of the zebrafish cyclin B1 ( ccnb1 ) gene upstream of the EGFP gene in the Tol2 vector, which was used to generate the stable Tg ( ccnb1:EGFP ) nt18 transgenic zebrafish line. (
  • At 36 h post-fertilization, both the enhanced green fluorescent protein (EGFP) and proliferating cell nuclear antigen (PCNA) expressions were observed throughout the developing transgenic retina, but they became restricted to the circumferential marginal zone by five days post-fertilization. (
  • Large-scale mutagenesis screens to identify mutants that disrupt zebrafish eye and retinal development [ 7 , 27 , 28 , 32 , 33 ], and the generation of an assortment of transgenic lines [ 14 , 34 ], has revealed and will continue to uncover important molecules and pathways in the development, maintenance, and function of the vertebrate retina. (
  • By using a transgenic approach in zebrafish we address if the most frequently found DPR in human ALS/FTLD brain, the poly-Gly-Ala (poly-GA) protein, is toxic in vivo. (
  • We generated several transgenic UAS responder lines that express either 80 repeats of GGGGCC alone, or together with a translation initiation ATG codon forcing the translation of GA80-GFP protein upon crossing to a Gal4 driver. (
  • Zebrafish transgenic for the GGGGCC repeat lacking an ATG codon showed very mild toxicity in the absence of poly-GA. However, strong toxicity was induced upon ATG initiated expression of poly-GA, which was rescued by injection of an antisense morpholino interfering with start codon dependent poly-GA translation. (
  • These novel transgenic C9orf72 associated repeat zebrafish models demonstrate poly-GA toxicity in zebrafish. (
  • Moreover, we detected a decrease in total protein ubiquitination, while the level of β‑catenin was higher in the striatum of HD transgenic mice as compared to wild-type mice. (
  • In zebrafish, however, mutants with disrupted notochord development lack a specific muscle cell type, the muscle pioneers, although they retain the ability to induce neural differentiation, raising the possibility that neural tube and somite patterning may be mediated by distinct signals. (
  • In crb2a mutants, we observed novel and severe defects in CM arrangement and morphology, as well as mislocalization of both TJ and AJ proteins. (
  • In contrast, zebrafish mutants bearing the previously identified mttp stl mutation (L475P) are deficient in transferring both triglycerides and phospholipids and exhibit gross intestinal lipid accumulation and defective growth. (
  • Zebrafish floating head ( flh ) mutants lack a notochord, but develop with prechordal plate and other mesodermal derivatives, indicating that flh functions specifically in notochord development. (
  • Germ cell loss occurs upon sexual maturation due to meiotic arrest, and zebrafish vasa mutants eventually become sterile males through a p53-independent process. (
  • Zebrafish rbpms2a;2b (rbpms2) double mutants develop exclusively as fertile males. (
  • Thus, the mpo gene should provide a useful molecular probe for identifying zebrafish mutants with defects in granulopoiesis. (
  • Hox proteins are transcriptional regulators that specify cell fate during early embryonic development and organogenesis (reviewed by Krumlauf, 1994 ). (
  • Tyrosine phosphorylation of proteins at two different stages in zebrafish embryonic development was studied by immuno-affinity purification and multi-dimensional LC-MS. Tyrosine kinases including members of the Src family of kinases, Eph receptors and Focal adhesion kinase as well as adaptor proteins such as Paxillin, Crk and p130Cas were identified. (
  • Hedgehog proteins play critical roles in organizing the embryonic development of animals, largely through modulation of target gene expression. (
  • Embryonic development is governed by highly coordinated changes in the expression of large protein sets. (
  • This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by correlating super-resolution light microscopy and scanning electron microscopy images. (
  • We present a method to investigate the subcellular protein localization in the larval zebrafish retina by combining super-resolution light microscopy and scanning electron microscopy. (
  • At 96 and 120 hpf, when the larval retina is fully differentiated and mdka expression is restricted to Müller glia and horizontal cells, Mdka protein is in the outer nuclear layer, but does not co-localize with cone-specific markers or the Müller glia marker glutamine synthetase. (
  • Matching the complex pattern of gene regulation, Mdka protein localization follows a complex temporal and spatial pattern in the zebrafish retina. (
  • Here we took advantage of the zebrafish retina to investigate the role of the cellular retinaldehyde-binding protein CRALBP in this process. (
  • These paralogues are differentially expressed in the zebrafish retina. (
  • Differential expression of Cralbp paralogues in the zebrafish retina as well as results obtained from behavioral and physiological studies of Cralbp-a/-b deficient larvae provide strong evidence for the existence of two pathways of visual pigment recycling in the zebrafish. (
  • The spatial and temporal patterning of EGFP expression in the Tg ( ccnb1:EGFP ) nt18 line directly reflects the known locations of proliferating cells in the zebrafish retina, making it a useful marker to study the transient nature of neuronal progenitor cells during the development and regeneration of the zebrafish retina. (
  • Evolution of complexity in the zebrafish synapse proteome. (
  • Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS," Proteomics , vol. 7, no. 4, pp. 484-493, 2007. (
  • A quantitative study of the effects of chaotropic agents, surfactants, and solvents on the digestion efficiency of human plasma proteins by trypsin," Journal of Proteome Research , vol. 9, no. 10, pp. 5422-5437, 2010. (
  • The Fralin Biomedical Research Institute scientists team used larval zebrafish to reveal a connection between seizures and a deficit in the brain protein connexin 36. (
  • Pan, who frequently uses larval zebrafish in studies, said the fish are ideal because they develop outside the womb, are translucent, and their entire brains are small enough to fit entirely under a microscope. (
  • Homologs of BefA are present in several human-associated bacterial species, and we show that they have conserved capacity to stimulate β cell proliferation in larval zebrafish. (
  • Light blue beta cells stimulated by the bacterial protein BefA can be seen in this image of a six-day old larval zebrafish stained with a dye that labels DNA. (
  • However, the gene editing efficiencies of Cas9 proteins with a nuclear localization signal (NLS) fused to different termini and Cas9 mRNA have not been systematically compared. (
  • Here, we compared the ability of Cas9 proteins with NLS fused to the N-, C-, or both the N- and C-termini and N-NLS-Cas9-NLS-C mRNA to target two sites in the tyr gene and two sites in the gol gene related to pigmentation in zebrafish. (
  • Simple, inexpensive and reliable procedures to visualize sites of protein production are required to allow observation of the spatiotemporal patterns of mRNA translation at subcellular resolution. (
  • We analyzed mRNA processing in seven zebrafish lines with mutations expected to disrupt gene function, generated by CRISPR/Cas9 or ENU mutagenesis methods. (
  • Should these mutations have the predicted effect on the mRNA and encoded protein, we would expect many more abnormal phenotypes than are typically being seen in reverse genetic screens. (
  • Tralopyril specifically induced the upregulation of 6 proteins implicated in energy metabolism, cytoskeleton, cell division and mRNA splicing whilst TPBP lead to the upregulation of 3 proteins involved in cytoskeleton, cell growth and protein folding. (
  • The specificity of bone morphogenetic protein (BMP) actions was confirmed by short interfering RNA knockdown of zfBMP4 expression in the CHO cells. (
  • This phenotype was reproduced in BMPR2 dominant-negative zebrafish and following knockdown of the zebrafish homologues of BMPR2 , bmpr2a and bmpr2b with morpholinos. (
  • We have carried out morpholino knockdown of fxr1 and have demonstrated abnormalities of striated muscle development and abnormal development of the zebrafish heart, including failure of looping and snapping of the atrium from its venous pole. (
  • We show that the specific knockdown of zebrafish papp-a limits the developmental rate beginning during gastrulation without affecting the normal patterning of the embryo. (
  • Mutations in the bone morphogenetic protein (BMP) type II receptor (BMPR-II) underlie heritable forms of the disease but the mechanisms leading to vascular disease remain obscure from studies in mice and humans. (
  • The lau mutations disrupt gpr126, an adhesion class G protein-coupled receptor gene. (
  • 5-9 Chemical mutagenesis screens in this organism have resulted in the isolation of numerous zebrafish lines containing mutations that affect both embryonic and definitive erythropoiesis. (
  • Here we set out to explore some of the reasons for this discrepancy by studying seven separate mutations in zebrafish. (
  • It is not known what role Meis proteins play in these complexes, nor is it clear which Hox functions require Meis proteins in vivo. (
  • To identify BMP responsive transcripts from vascular endothelial cells in vivo we developed a system to allow FACS isolation of GFP+ve cells dissociated from Tg( fli1a:egfp ) zebrafish following incubation in LDN193189 or DMSO solvent control. (
  • Moreover, APOL1 G2, but not G1, expression alone promotes developmental kidney defects, suggesting a possible dominant-negative effect of the altered protein.Testing this interaction in vivo by co-suppressing both transcripts yielded no additive effects.Furthermore, concordant with the genetic interaction observed in SCD patients, APOL1 G2 reduces myh9 expression in vivo, suggesting a possible interaction between the altered APOL1 and myh9. (
  • In vivo complementation studies of the G2 risk allele also indicate that the variant is deleterious to protein function. (
  • Therefore we have explored the possibilities to use zebrafish development as a model system to study in vivo phosphorylation using mass spectrometry. (
  • The results established zebrafish as a good model to study in vivo phosphorylation and reveal that surprising homology exists with human phosphoproteins. (
  • 2004) VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. (
  • These data add to our understanding of structure-function relationships in Na + channel β1 subunits and establish zebrafish as an ideal system in which to determine the contribution of scn1ba to electrical excitability in vivo . (
  • To test this, we performed in vivo imaging of zebrafish expressing a fluorescent reporter protein under the control of the myelin basic protein (mbp) promoter. (
  • Using a timelapse assay in the zebrafish retinotectal system, we demonstrate that the β-actin 3'UTR is sufficient to target local translation of the photoconvertible fluorescent protein Kaede in growth cones of pathfinding retinal ganglion cells (RGCs) in vivo. (
  • The antisense morpholino technique was employed to create zebrafish larvae lacking Cralbp-a or -b protein. (
  • The in vitro kinase chip technology was also used to determine protein tyrosine kinase (PTK) activity profiles from morpholino mediated knock down zebrafish embryo lysates. (
  • Surprisingly, 11 of the 12 lines were each heterozygous for a mutation in a different ribosomal protein (RP) gene, while one line was heterozygous for a mutation in a zebrafish paralog of the human and mouse tumor suppressor gene, neurofibromatosis type 2. (
  • examined a zebrafish variant with a genetic mutation that disrupts the function of a protein called Tight Junction Protein 1a (or Tjp1a)-a fish variant of a mammalian protein called ZO-1. (
  • We have discovered a zebrafish mutant ( mttp c655 ) expressing a C-terminal missense mutation (G863V) in Mttp, one of the two subunits of MTP, that is defective at transferring triglycerides, but retains phospholipid transfer activity. (
  • Thus, the G863V point mutation provides the first evidence that the triglyceride and phospholipid transfer functions of a vertebrate MTP protein can be separated, arguing that selective inhibition of the triglyceride transfer activity of MTP may be a feasible therapeutic approach for dyslipidemia. (
  • Here we describe the identification, mapping and cloning of a zebrafish carbonic anhydrase 5 (ca5) mutation, collapse of fins (cof), which causes initially a collapse of the medial fins followed by necrosis and rapid degeneration of the embryo. (
  • 11 Furthermore, the cloning of the reisling mutation, which causes a defect in zebrafish erythrocytes analogous to that found in human hereditary spherocytosis, led to the identification of zebrafish β-spectrin . (
  • With assistance from Duke researchers in Christopher Nicchitta's group, who specialize in the mechanics of protein translation, the team was even able to start investigating the specific effects of the mutation that might lead to smaller head size: when zebrafish rpl10 expression was suppressed, the animals showed reduced protein production and increased cell death within the brain. (
  • We address this issue by analyzing the Xenopus and zebrafish homologs of Foxj1, a forkhead transcription factor necessary for ciliogenesis in multiciliated cells of the mouse. (
  • We have identified two highly conserved zebrafish homologs of the human PAPP-A gene. (
  • In vertebrates, three distinct Ci homologs, the Gli proteins, have subsumed the function of Ci in the regulation of target gene expression. (
  • However, various names were assigned to these vertebrate homologs, which complicated the nomenclature of teneurin proteins. (
  • Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY). (
  • Model organism zebrafish is relevant to higher vertebrates significantly, with highly manageable genetic manipulation, which making it an exceptional animal model for exploring molecular mechanism regulating key developmental processes. (
  • Because Drosophila Ci and the Gli3, and possibly Gli2, proteins of vertebrates undergo phosphorylation and proteolytic processing in the absence of Hh to yield truncated transcriptional repressor forms, optimal induction of target gene expression is critically determined by the ratio of the activator (Gli act ) vs. the repressor variants (Gli rep ) of these proteins within the nucleus. (
  • Since discovery of teneurins in Drosophila, many other laboratories have independently described the Ten-a and Ten-m/Odz homolog proteins in different vertebrates. (
  • The primary structure, or amino acid sequence identity, of the proteins between paralogs is ~60% identical and between orthologs is ~90%, whilst between vertebrates and Drosophila or C. elegans is only 33-41% identical. (
  • These bacteria share a gene that encodes a previously undescribed protein, named herein BefA (β Cell Expansion Factor A), which is sufficient to induce β cell proliferation in developing zebrafish larvae. (
  • Normally, the number of beta cells in zebrafish larvae increases steadily in the first few days after hatching. (
  • Expressing either protein under the control of the other's regulatory sequences resulted in a WT phenotype, providing strong evidence that these two proteins are functionally equivalent despite their different single mutant phenotypes and diverged protein sequences. (
  • We have cloned a zebrafish mutant, lauscher (lau), identified by its swollen ear phenotype. (
  • Moreover, our interaction studies indicate that the MYH9 locus is also relevant to the phenotype in a stressed microenvironment and suggest that consideration of the context-dependent functions of both proteins will be required to develop therapeutic paradigms. (
  • Furthermore, a comparison of blood phenotypes of sparc and fgfs knockdowns with similar defects and the sparc rescue of fgf21 blood phenotype places sparc downstream of fgf21 in the genetic network regulating haematopoiesis in zebrafish. (
  • This phenotype is different from those resulting from deficiency of Igf receptor or ligand in zebrafish, suggesting a function of Papp-a outside the Igf system. (
  • Researchers at the University of Oregon have identified a novel bacterial protein that induces pancreatic beta cell proliferation during zebrafish development. (
  • Moreover, APOL1 G2, but not G1, expression alone promotes developmental kidney defects, suggesting a possible dominant-negative effect of the altered protein. (
  • These results shed new insights into the mechanisms by which fish species defend themselves against vast numbers of xenobiotics via glucuronidation conjugations and may facilitate the establishment of zebrafish as a model vertebrate in toxicological, developmental, and pathologic studies. (
  • Thus, this method may be used more widely for the study of developmental protein stability. (
  • In this dissertation, I have characterized the functions of two RNAbps, Vasa and Rbpms2, in the development of the zebrafish germline. (
  • We (humans) share 70 per cent of our genetic code with zebrafish, and the human CTGF protein is nearly 90% similar to the one from the zebrafish. (
  • combined with the optical transparency and sophisticated genetic tools of the zebrafish model, make it a powerful platform to investigate this question. (
  • Genetic data link these phenotypes to insulin-like growth factor binding protein-3 (Igfbp-3) and Bmp signaling, and biochemical analysis show specific Igfbp-3 proteolysis by Papp-a2, implicating Papp-a2 in the modulation of Bmp signaling by Igfbp-3 proteolysis. (
  • This new resource should facilitate the selection of candidate proteins for targeted quantitation and refine systematic genetic network analysis in vertebrate development and biology. (
  • The morphologic and molecular genetic evidence presented here supports the zebrafish as an informative model system for the study of normal and aberrant human myelopoiesis. (
  • The Thyme lab ( uses zebrafish as a model system to understand the genetic mechanisms underlying psychiatric and neurodevelopmental disease. (
  • This gene encodes part of the ribosome, which is a central piece of the molecular machinery that translates the genetic code into proteins. (
  • The zebrafish is a well-established genetic model. (
  • This first proteomic analysis of the zebrafish skeleton revealed the homology between the zebrafish and the skeleton of other vertebrate species including mammals. (
  • Mammals cannot regenerate their complex nervous tissue but small, tropical zebrafish can repair a severed spine in eight weeks. (
  • The zebrafish has a well-characterized program of β cell development, which is highly conserved with that of mammals ( Kinkel and Prince, 2009 ). (
  • As zebrafish PKCs are expressed in structures that are equivalent to mammals, the zebrafish model is well suited to study evolutionary conserved functions of PKCs in development and disease. (
  • 7 18 19 The earliest site of zebrafish hematopoiesis is the ICM, a region analogous to the blood islands of the yolk sac in mammals. (
  • In order to understand the potential functions of this gene in vertebrate development, we cloned the zebrafish orthologue of human ZNF230, named rnf141. (
  • Expression of the zebrafish orthologue of human TRA2B , tra2b , that encodes a protein binding to MAPT transcripts and regulating splicing, was reduced under hypoxic conditions, similar to observations in AD brain. (
  • At the molecular level, the biological similarity between zebrafish and humans is striking (Spoorendonk et al. (
  • Molecular targets of TBBPA in zebrafish analysed through integration of genomic and proteomic approaches," Chemosphere , vol. 74, no. 1, pp. 96-105, 2008. (
  • The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. (
  • Because the encoded zebrafish UDP glucuronosyltransferase (UGT) proteins do not display orthologous relationships to any of the mammalian and avian UGT enzymes based on molecular phylogeny, it is difficult to predict their substrate specificity. (
  • Somatotropin Zebrafish Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 185 amino acids with an additional Ala at the N-terminus and having a molecular mass of 21.18 kDa. (
  • Recently, several lysin motif (LysM)-containing proteins were highlighted for their molecular affinity to chitin-like compounds. (
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (
  • section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. (
  • At the amino acid sequence level, mature human, mouse, and rat BMP-2 are 100% identical, while mature human BMP-2 and zebrafish BMP-2a are 85% identical. (
  • Zebrafish BMP-4 protein shares 73% amino acid sequence identity with human and murine BMP-4, and 86% amino acid sequence identity with zebrafish BMP-2b. (
  • Human and zebrafish DYRK1A protein sequence possess 75.6% similarity and same function domains, suggesting the evolutional conservation. (
  • A DNA sequence encoding the zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified. (
  • We detected 1384 proteins from 327,906 peptide sequence identifications at 72 and 120 hpf with false identification rates of less than 1% using 2D LC-ESI-MS/MS. These included only ∼30% of proteins that were identified by 2D PAGE-MALDI-TOF/TOF. (
  • MafT protein bound MARE sequence as a homodimer or heterodimers with zebrafish Nrf2 or p45 Nfe2. (
  • Also in 1998, Sergei Kiselev and coworkers independently discovered and cloned a protein from a mouse adenocarcinoma with the same sequence as PGRP, which they named Tag7. (
  • A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (
  • The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (
  • Both scn1ba splice forms modulate Na + currents expressed by zebrafish scn8aa , resulting in shifts in channel gating mode, increased current amplitude, negative shifts in the voltage dependence of current activation and inactivation, and increases in the rate of recovery from inactivation, similar to the function of mammalian β1 subunits. (
  • In contrast to mammalian β1, however, neither zebrafish subunit produces a complete shift to the fast gating mode and neither subunit produces complete channel inactivation or recovery from inactivation. (
  • 4 Homologues of known mammalian hematopoietic transcription factors, such as gata-1, gata-2, c-myb , scl, fli-1, Imo2 , and cbfb are all appropriately expressed during zebrafish embryogenesis. (
  • Mammalian PGRPs are secreted proteins that typically form dimers and contain either one PGRP domain (e.g., human PGLYRP1 and PGLYRP2) or two PGRP domains (e.g., human PGLYRP3 and PGLYRP4). (
  • In addition, Npc1 (Niemann-Pick disease, type C1), a 13 transmembrane-spanning protein containing a sterol-sensing domain, was also demonstrated to contribute early morphogenetic movements including epiboly and CE movements [ 7 ]. (
  • Background-The single spanning transmembrane amyloid precursor protein (APP) and its proteolytic product, amyloid-beta (Aβ) peptide, have been intensely studied due to their role in the pathogenesis of Alzheimer's disease. (
  • citation needed] In zebrafish, the PGCs express two CXCR4 transmembrane receptor proteins. (
  • All teneurins, especially in mouse, are type II transmembrane proteins that are composed of a large extracellular C terminal domain of ~2400 amino acid residues, a single transmembrane helical domain of ~30 hydrophobic residues and an intracellular N terminal domain of ~300-375 residues. (
  • We herein report the expression profiles of zebrafish bmp3 ( zbmp3 ) as demonstrated by real-time PCR and in situ hybridization. (
  • To further understand the function of LP during the process of chondrogenesis and bone formation in zebrafish, we cloned the zebrafish cDNA for hyaluronan and proteoglycan link protein 1 (crtl1/hapln1) and examined the expression of the gene during embryogenesis using in-situ hybridization. (
  • The mechanical properties of the skeleton are largely dependent on the composition of proteins that are secreted into the extracellular matrix (ECM). (
  • The aim of this thesis was to analyze the composition of the extracellular matrix in zebrafish, in order to enable the identification of potential key regulatory proteins. (
  • In zebrafish and Xenopus, semicircular canal ducts develop when projections of epithelium, driven by extracellular matrix production, push into the otic vesicle and fuse to form pillars. (
  • We show that in the zebrafish, extracellular matrix gene expression is high during projection outgrowth and then rapidly downregulated after fusion. (
  • We propose that Gpr126 acts through a cAMP-mediated pathway to control the outgrowth and adhesion of canal projections in the zebrafish ear via the regulation of extracellular matrix gene expression. (
  • These results establish a role for an extracellular matrix protein (Sparc) as an important regulator of embryonic haematopoiesis during early development in zebrafish. (
  • However, the role of the extracellular matrix (ECM) during zebrafish heart regeneration has been comparatively rarely explored. (
  • To improve the experimental methods, he developed nanotechnological strategies to genetically modify human embryonic kidney cells and zebrafish. (
  • Over the past 15 years, Guillemin and co-workers have developed methods for growing germ-free zebrafish, allowing them to ask what happens when the animals develop in the absence of microbes. (
  • Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease. (
  • Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish," Mayssa H. Mokalled, Chinmoy Patra, Amy L. Dickson, Toyokazu Endo, Didier Y. R. Stainier, Kenneth D. Poss. (
  • The zebrafish is known for its spectacular abilities to regenerate, including being able to repair its own spinal cord. (
  • Using this system, we have investigated the role of cAMP protein kinase A (PKA) signaling in pigment cell differentiation. (
  • GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. (
  • This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). (
  • Adaptor proteins CT10 regulator of kinase (CRK) and CRK-like are known to be important for proper migration of neurons in the developing cortex through their role in the Reelin signaling pathway. (
  • Kinase reactions were performed using complex zebrafish embryo lysates or purified kinases. (
  • In conclusion, these data link pappalysin function in zebrafish to two different signaling pathways outside the IGF system. (
  • Two orthologues of the CRALBP gene (CRALBP-a, CRALBP-b) were cloned in the zebrafish. (
  • We previously identified two paralogues (co-orthologues) of MAPT in zebrafish, mapta and maptb . (
  • Overall, our findings indicate that hypoxia can alter splicing of zebrafish MAPT co-orthologues promoting formation of longer transcripts and possibly generating Mapt proteins more prone to hyperphosphorylation. (
  • Effects of penthiopyrad on the development and behaviour of zebrafish in early-life stages. (
  • Here, we present a "closed-loop control" system to investigate the behaviour of zebrafish, a popular animal model in preclinical studies. (
  • In this report, we demonstrate that zebrafish hoxa3a ( zfhoxa3a ) expressed from the mouse Hoxa3 locus can substitute for mouse Hoxa3 in some tissues, but has distinct or null phenotypes in others. (
  • Em = 5 dpf whole-zebrafish embryo cDNA. (
  • The embryonic Drosophila cDNA library was screened using polymerase chain reaction (PCR) and a primer derived from the EGF-like repeats region of chicken tenascin-C protein. (
  • In order to explore the role of cpz in zebrafish development, a loss-of-function mutant for zebrafish cpz was generated via TALEN-mediated mutagenesis. (
  • Mutagenesis of the conserved glycine in the human MTTP protein (G865V) also eliminates triglyceride but not phospholipid transfer activity. (
  • The use of zebrafish as a model in future research will undoubtedly increase our understanding of vertebrate skeletal development and disease. (
  • Zebrafish serve as a powerful animal model, allowing researchers to evaluate the effects of connexin 36 on the whole brain in an intact living system during neural hyperactivity. (
  • I thought, because the zebrafish is such a versatile model organism, we could use it to try to discern what actually is going on. (
  • Researchers consider that zebrafish can be used as a model to study in deep the strategies to treat this type of diseases. (
  • Here we use zebrafish, which have a well-documented pattern of angiogenesis, as a model organism to address this question. (
  • The zebrafish is an ideal model to investigate the role of fxr1 during development of the heart. (
  • Here, we used zebrafish model to detect the definite role of excessive expression of DYRK1A in PGCs development during embryogenesis. (
  • While several studies have demonstrated the toxicity of these proteins, not much is known about the effect on motor function in a vertebrate model. (
  • We present a convenient method that allows the analysis and comparison of protein stabilities during embryogenesis using early zebrafish development as a model system. (
  • For example, possible applicants could include a biochemist who wants to gain experience with a model organism or anyone with zebrafish expertise who wants to think more about the details of proteins underlying neurodevelopmental disorders. (
  • Applicants could also include someone with experience in a different model system, such as Drosophila or C. Elegans, who is interested in developing skills with zebrafish. (
  • Using germ-free zebrafish as a model, the team showed that certain gut bacteria are necessary for the pancreas to populate itself with a robust number of beta cells during development. (
  • AIM(S): To determine the effect of increased dimerization of CacyBP/SIP protein in YAC128 model and the effect of decreased level of Cacybp in zebrafish on β‑catenin signaling. (
  • However, given that Hox proteins are transcription factors it seems likely that Meis and Pbx might also contribute functions that regulate the transcriptional activity of the complexes. (
  • The Id proteins (Inhibitors of DNA Binding/Differentiation) represent a family of dominant negative regulators of the basic helix-loop-helix transcription factors whose activities result in delayed cell differentiation and prolonged proliferation. (
  • Previous data from our lab have demonstrated that deficiency of Crk and Crkl during development negatively impacts eye size and retinal lamination in Zebrafish. (
  • To create the Tg ( ccnb1:EGFP ) nt18 zebrafish line that spatially and temporally labels retinal progenitor cells with enhanced green fluorescent protein (EGFP) during zebrafish retinal development and regeneration. (
  • In this thesis we provide a first insight in the extracellular protein content of the zebrafish skeleton, identify a role for the Cpz protein in β-catenin independent (non-canonical) Wnt signaling during development and shows the effect of thyroid hormone on ossification during early zebrafish development. (
  • We used zebrafish embryo lysates to determine PTK activity profiles, thus providing the unique opportunity to directly compare the effect of protein knock downs on PTK activity profiles on the one hand and phenotypic changes on the other. (
  • By comparing normal, wild-type zebrafish and mutant zebrafish with connexin 36 deficiencies, they found that connexin 36 deficiency altered the susceptibility to neuronal hyperactivity in a brain-region and drug-dose dependent manner. (
  • Biochemical analysis of recombinant zebrafish Papp-a demonstrates conservation of proteolytic activity, specificity, and intrinsic regulatory mechanism. (
  • Small Maf proteins play critical roles on morphogenesis and homeostasis through associating with CNC proteins. (
  • Conclusion BMP signalling, via BMPR2 , is critical for venous but not arterial angiogenesis in zebrafish. (
  • VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. (
  • As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation of core subunits of the oxidative phosphorylation system. (
  • A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (
  • Mapping protein phosphorylation in zebrafish development Reversible protein phosphorylation plays a key role in signaling processes that are vital for a cell and organism. (
  • As a consequence protein phosphorylation and the elucidation of signaling pathways have received considerable attention over the past years. (
  • The predicted protein encoded by cyp26d1 consists of 554 amino acids. (
  • It exhibits 54% amino acid identity with human Cyp26C1, 50% with zebrafish Cyp26B1 and 38% with zebrafish Cyp26A1. (
  • As a result of alternative splicing of MAPT transcripts, six tau protein isoforms ranging from 352 to 441 amino acid residues in length are generated and expressed in the human brain. (
  • The respiratory quotient increased slightly (0.89 to 0.97) whereas the nitrogen quotient increased greatly (0.072 to 0.140), representing peak amino acid/protein usage (52%) at this time. (
  • After 48-h fasting, endogenous carbohydrate and lipid were the major fuels, but in the first few hours after feeding, carbohydrate oxidation increased greatly, fueling the first part of the post-prandial specific dynamic action, whereas increased protein/amino acid usage predominated from 6 h onwards. (
  • Excess dietary protein/amino acids were preferentially metabolized for energy production. (
  • However, based on an analysis of the literature, Wood (2001) speculated that in most teleosts, lipid and carbohydrate are probably the major fuels burned during fasting (i.e. after absorption of nutrients from a meal is complete), but that protein/amino acid oxidation may increase greatly after feeding. (
  • In theory, the relative ratios of Ṁ O 2 , Ṁ CO 2 and Ṁ N can be used to stoichiometrically calculate the particular combination of carbohydrate, lipid and protein/amino acids being oxidized at any point in time ( Kleiber, 1987 , 1992 ). (
  • Although no transcription regulatory functions have been found for Meis proteins, the Meis homeodomain is not required for all Meis functions (e.g. (
  • The homeodomain transcription factor Six1 and its modulator, the protein phosphatase Eya1, cooperate to promote cell differentiation and survival during mouse organ development. (
  • Here, we show that zebrafish hearts lacking Crb2a, a component of the Crumbs polarity complex, display compact wall integrity defects and fail to form trabeculae. (
  • siRNA silencing of EWSR1 in Hela cells resulted in mitotic defects accompanied by apoptotic cell death, indicating that the role of EWSR1 is conserved between zebrafish and human. (
  • Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins that are functionally important during vertebrate development. (