An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).
Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.
The observable response an animal makes to any situation.
Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
The protection of animals in laboratories or other specific environments by promoting their health through better nutrition, housing, and care.
The study of the actions and properties of medicinal agents, often derived from PLANTS, indigenous to populations or ETHNIC GROUPS.
The study of medicines derived from botanical sources.
The process of finding chemicals for potential therapeutic use.
The use of animals as investigational subjects.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Individual's rights to obtain and use information collected or generated by others.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.
A subspecialty of internal medicine concerned with morphology, physiology, and pathology of the blood and blood-forming tissues.
A cartilaginous rod of mesodermal cells at the dorsal midline of all CHORDATE embryos. In lower vertebrates, notochord is the backbone of support. In the higher vertebrates, notochord is a transient structure, and segments of the vertebral column will develop around it. Notochord is also a source of midline signals that pattern surrounding tissues including the NEURAL TUBE development.
Genes that encode highly conserved TRANSCRIPTION FACTORS that control positional identity of cells (BODY PATTERNING) and MORPHOGENESIS throughout development. Their sequences contain a 180 nucleotide sequence designated the homeobox, so called because mutations of these genes often results in homeotic transformations, in which one body structure replaces another. The proteins encoded by homeobox genes are called HOMEODOMAIN PROTEINS.
The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.
The only species of a cosmopolitan ascidian.
The processes occurring in early development that direct morphogenesis. They specify the body plan ensuring that cells will proceed to differentiate, grow, and diversify in size and shape at the correct relative positions. Included are axial patterning, segmentation, compartment specification, limb position, organ boundary patterning, blood vessel patterning, etc.
Aquatic vertebrate sensory system in fish and amphibians. It is composed of sense organs (canal organs and pit organs) containing neuromasts (MECHANORECEPTORS) that detect water displacement caused by moving objects.
An oil from flower buds of SYZYGIUM trees which contains large amounts of EUGENOL.
One of the Liliaceae used as a spice (SPICES) and traditional remedy. It contains alliin lyase and alliin, which is converted by alliin lyase to allicin, the pungent ingredient responsible for the aroma of fresh cut garlic.
A continuing periodic change in displacement with respect to a fixed reference. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.

The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping. (1/8702)

Left-right asymmetry in vertebrates is controlled by activities emanating from the left lateral plate. How these signals get transmitted to the forming organs is not known. A candidate mediator in mouse, frog and zebrafish embryos is the homeobox gene Pitx2. It is asymmetrically expressed in the left lateral plate mesoderm, tubular heart and early gut tube. Localized Pitx2 expression continues when these organs undergo asymmetric looping morphogenesis. Ectopic expression of Xnr1 in the right lateral plate induces Pitx2 transcription in Xenopus. Misexpression of Pitx2 affects situs and morphology of organs. These experiments suggest a role for Pitx2 in promoting looping of the linear heart and gut.  (+info)

oko meduzy mutations affect neuronal patterning in the zebrafish retina and reveal cell-cell interactions of the retinal neuroepithelial sheet. (2/8702)

Mutations of the oko meduzy (ome) locus cause drastic neuronal patterning defect in the zebrafish retina. The precise, stratified appearance of the wild-type retina is absent in the mutants. Despite the lack of lamination, at least seven retinal cell types differentiate in oko meduzy. The ome phenotype is already expressed in the retinal neuroepithelium affecting morphology of the neuroepithelial cells. Our experiments indicate that previously unknown cell-cell interactions are involved in development of the retinal neuroepithelial sheet. In genetically mosaic animals, cell-cell interactions are sufficient to rescue the phenotype of oko meduzy retinal neuroepithelial cells. These cell-cell interactions may play a critical role in the patterning events that lead to differentiation of distinct neuronal laminae in the vertebrate retina.  (+info)

A Drosophila doublesex-related gene, terra, is involved in somitogenesis in vertebrates. (3/8702)

The Drosophila doublesex (dsx) gene encodes a transcription factor that mediates sex determination. We describe the characterization of a novel zebrafish zinc-finger gene, terra, which contains a DNA binding domain similar to that of the Drosophila dsx gene. However, unlike dsx, terra is transiently expressed in the presomitic mesoderm and newly formed somites. Expression of terra in presomitic mesoderm is restricted to cells that lack expression of MyoD. In vivo, terra expression is reduced by hedgehog but enhanced by BMP signals. Overexpression of terra induces rapid apoptosis both in vitro and in vivo, suggesting that a tight regulation of terra expression is required during embryogenesis. Terra has both human and mouse homologs and is specifically expressed in mouse somites. Taken together, our findings suggest that terra is a highly conserved protein that plays specific roles in early somitogenesis of vertebrates.  (+info)

Isolation of zebrafish gdf7 and comparative genetic mapping of genes belonging to the growth/differentiation factor 5, 6, 7 subgroup of the TGF-beta superfamily. (4/8702)

The Growth/differentiation factor (Gdf) 5, 6, 7 genes form a closely related subgroup belonging to the TGF-beta superfamily. In zebrafish, there are three genes that belong to the Gdf5, 6, 7 subgroup that have been named radar, dynamo, and contact. The genes radar and dynamo both encode proteins most similar to mouse GDF6. The orthologous identity of these genes on the basis of amino acid similarities has not been clear. We have identified gdf7, a fourth zebrafish gene belonging to the Gdf5, 6, 7 subgroup. To assign correct orthologies and to investigate the evolutionary relationships of the human, mouse, and zebrafish Gdf5, 6, 7 subgroup, we have compared genetic map positions of the zebrafish and mammalian genes. We have mapped zebrafish gdf7 to linkage group (LG) 17, contact to LG9, GDF6 to human chromosome (Hsa) 8 and GDF7 to Hsa2p. The radar and dynamo genes have been localized previously to LG16 and LG19, respectively. A comparison of syntenies shared among human, mouse, and zebrafish genomes indicates that gdf7 is the ortholog of mammalian GDF7/Gdf7. LG16 shares syntenic relationships with mouse chromosome (Mmu) 4, including Gdf6. Portions of LG16 and LG19 appear to be duplicate chromosomes, thus suggesting that radar and dynamo are both orthologs of Gdf6. Finally, the mapping data is consistent with contact being the zebrafish ortholog of mammalian GDF5/Gdf5.  (+info)

Sequential PKC- and Cdc2-mediated phosphorylation events elicit zebrafish nuclear envelope disassembly. (5/8702)

Molecular markers of the zebrafish inner nuclear membrane (NEP55) and nuclear lamina (L68) were identified, partially characterized and used to demonstrate that disassembly of the zebrafish nuclear envelope requires sequential phosphorylation events by first PKC, then Cdc2 kinase. NEP55 and L68 are immunologically and functionally related to human LAP2beta and lamin B, respectively. Exposure of zebrafish nuclei to meiotic cytosol elicits rapid phosphorylation of NEP55 and L68, and disassembly of both proteins. L68 phosphorylation is completely inhibited by simultaneous inhibition of Cdc2 and PKC and only partially blocked by inhibition of either kinase. NEP55 phosphorylation is completely prevented by inhibition or immunodepletion of cytosolic Cdc2. Inhibition of cAMP-dependent kinase, MEK or CaM kinase II does not affect NEP55 or L68 phosphorylation. In vitro, nuclear envelope disassembly requires phosphorylation of NEP55 and L68 by both mammalian PKC and Cdc2. Inhibition of either kinase is sufficient to abolish NE disassembly. Furthermore, novel two-step phosphorylation assays in cytosol and in vitro indicate that PKC-mediated phosphorylation of L68 prior to Cdc2-mediated phosphorylation of L68 and NEP55 is essential to elicit nuclear envelope breakdown. Phosphorylation elicited by Cdc2 prior to PKC prevents nuclear envelope disassembly even though NEP55 is phosphorylated. The results indicate that sequential phosphorylation events elicited by PKC, followed by Cdc2, are required for zebrafish nuclear disassembly. They also argue that phosphorylation of inner nuclear membrane integral proteins is not sufficient to promote nuclear envelope breakdown, and suggest a multiple-level regulation of disassembly of nuclear envelope components during meiosis and at mitosis.  (+info)

Comparative synteny cloning of zebrafish you-too: mutations in the Hedgehog target gli2 affect ventral forebrain patterning. (6/8702)

Zebrafish you-too (yot) mutations interfere with Hedgehog (Hh) signaling during embryogenesis. Using a comparative synteny approach, we isolated yot as a zinc finger transcription factor homologous to the Hh target gli2. Two alleles of yot contain nonsense mutations resulting in carboxy-terminally truncated proteins. In addition to causing defects in midline development, muscle differentiation, and retinal axon guidance, yot mutations disrupt anterior pituitary and ventral forebrain differentiation. yot mutations also cause ectopic lens formation in the ventral diencephalon. These findings reveal that truncated zebrafish Gli2 proteins interfere with Hh signaling necessary for differentiation and axon guidance in the ventral forebrain.  (+info)

Retinal neurogenesis: the formation of the initial central patch of postmitotic cells. (7/8702)

We have investigated the relationship between the birthdate and the onset of differentiation of neurons in the embryonic zebrafish neural retina. Birthdates were established by a single injection of bromodeoxyuridine into embryos of closely spaced ages. Differentiation was revealed in the same embryos with a neuron-specific antibody, zn12. The first bromodeoxyuridine-negative (postmitotic) cells occupied the ganglion cell layer of ventronasal retina, where they formed a small cluster of 10 cells or less that included the first zn12-positive cells (neurons). New cells were recruited to both populations (bromodeoxyuridine-negative and zn12-positive) along the same front, similar to the unfolding of a fan, to produce a circular central patch of hundreds of cells in the ganglion cell layer about 9 h later. Thus the formation of this central patch, previously considered as the start of retinal neurogenesis, was actually a secondary event, with a developmental history of its own. The first neurons outside the ganglion cell layer also appeared in ventronasal retina, indicating that the ventronasal region was the site of initiation of all retinal neurogenesis. Within a column (a small cluster of neuroepithelial cells), postmitotic cells appeared first in the ganglion cell layer, then the inner nuclear layer, and then the outer nuclear layer, so cell birthday and cell fate were correlated within a column. The terminal mitoses occurred in three bursts separated by two 10-h intervals during which proliferation continued without terminal mitoses.  (+info)

beta-thymosin is required for axonal tract formation in developing zebrafish brain. (8/8702)

beta-Thymosins are polypeptides that bind monomeric actin and thereby function as actin buffers in many cells. We show that during zebrafish development, &bgr;-thymosin expression is tightly correlated with neuronal growth and differentiation. It is transiently expressed in a subset of axon-extending neurons, essentially primary neurons that extend long axons, glia and muscle. Non-neuronal expression in the brain is restricted to a subset of glia surrounding newly forming axonal tracts. Skeletal muscle cells in somites, jaw and fin express beta-thymosin during differentiation, coinciding with the time of innervation. Injection of beta-thymosin antisense RNA into zebrafish embryos results in brain defects and impairment of the development of beta-thymosin-associated axon tracts. Furthermore, irregularities in somite formation can be seen in a subset of embryos. Compared to wild-type, antisense-injected embryos show slightly weaker and more diffuse engrailed staining at the midbrain-hindbrain boundary and a strong reduction of Isl-1 labeling in Rohon Beard and trigeminal neurons. The decreased expression is not based on a loss of neurons indicating that beta-thymosin may be involved in the maintenance of the expression of molecules necessary for neuronal differentiation. Taken together, our results strongly indicate that beta-thymosin is an important regulator of development.  (+info)

TY - JOUR. T1 - Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines. AU - Hashiguchi, Megumi. AU - Shinya, Minori. AU - Tokumoto, Mika. AU - Sakai, Noriyoshi. PY - 2008/9/15. Y1 - 2008/9/15. N2 - Formation of the dorsal organizer (Spemann organizer) is an important process in early vertebrate development. In zebrafish, two molecular cascades-Bozozok/Dharma (Boz) and Nodal signaling-act in parallel to induce the dorsal organizer. However, the complete molecular mechanism regulating this event remains unclear. Here we report that zebrafish cell lines derived from various developmental stages can induce a secondary axis when they are implanted into the mid-blastula but not the early gastrula. The implanted cellsthemselves did not differentiate, but instead induced ectopic expression of dorsal organizer markers incells around the implanted cells and induced notochord formation in the secondary axis. These results indicate that cultured cell lines have the ability to ...
View details for Studies on the effect of certain supplements in cryoprotective medium on zebrafish (Danio rerio) oocytes quality after controlled slow cooling..
The availability of a gynogenetic isogenic homozygous diploid clonal strain (C) of the zebrafish (Brachydanio rerio), combined with the small adult body size of the species, made possible a study of the following two questions. (1) Is the genetic uniformity of a group of fish reflected in decreased variability of features of organismic performance physiology? (2) Is the metabolic cost of subcarangiform swimming significantly different in small fishes compared with large ones? Wild-type (WT) and C strain zebrafish maintained at 28 °C can all swim very rapidly [up to relative swimming speeds of 13 body lengths s-1 (BL s-1)] for extended periods (at least 2 h) without visibly tiring. Oxygen consumption rates were measured for both types at swimming speeds of 1.5­13 BL s-1. Whole-body lactate concentrations were also measured during routine activity and after prolonged exercise for both fish types. The slopes of the linear regressions between the logarithm of mass-specific oxygen consumption ...
In this study we set out to investigate the transcriptome dynamics of early zebrafish embryogenesis with a comprehensive experimental set-up that interroga
Embryonic and larval zebrafish (Danio rerio) behavior is commonly used to identify neurotoxic compounds. Here, we investigated whether sub-lethal exposures to the common solvents dimethyl sulfoxide (DMSO, 0.01-1%) and methanol (MeOH, 0.01-1%), or the anti-fungal agent methylene blue (MB, 0.0001-0.0005%), can influence larval behavior in a simple light/dark paradigm conducted in 96-well plates. In addition, we tested whether the media volume within the behavioral arena or the zebrafish strain, AB wild type, AB Tübingen (AB/TU), or Tüpfel long-fin (TL), could also influence larval behavior. Following the single exposures, we co-exposed larvae to DMSO and either MB or two other compounds with known behavioral effects in larval zebrafish, flutamide and perfluorooctanesulfonic acid (PFOS). We found ≥0.55% DMSO and 0.0005% MB significantly affected larval behavior, but there was no effect of MeOH. Similarly, TL showed less movement compared to AB and AB/TU strains, whereas lower media volumes also ...
Rajaram K, Harding RL, Hyde DR, Patton JG. miR-203 regulates progenitor cell proliferation during adult zebrafish retina regeneration. Dev Biol. 2014 May 20. p
Live Imaging of Host-Parasite Interactions in a Zebrafish Infection Model Reveals Cryptococcal Determinants of Virulence and Central Nervous System Invasion.
Cell cycle analysis of transgenic zebrafish embryos expressing PCNA-CB. (A) Overview of the dorsal midbrain of a wnt1:gal4,UAS:GFP (green); UAS:PCNA-CB (magenta
BioAssay record AID 620365 submitted by ChEMBL: Antiangiogenic activity in fli-1:enhanced GFP expressing transgenic zebrafish embryo assessed as inhibition of neovacularisation at 5 uM after 24 hrs relative to control.
TY - JOUR. T1 - Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. AU - Tseng, Deng Yu. AU - Chou, Ming Yi. AU - Tseng, Yung Che. AU - Hsiao, Chung Der. AU - Huang, Chang Jen. AU - Kaneko, Toyoji. AU - Hwang, Pung Pung. PY - 2009/3/1. Y1 - 2009/3/1. N2 - Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca2+ uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca2+ uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) freshwater stimulated whole body Ca2+ influx and zebrafish epithelial Ca2+ channel (zECaC) mRNA expression, while downregulated ...
Claudin 5 as a prominent TJ protein is a consistent feature between the BBB and blood-CSF barrier (Bill and Korzh, 2014). Here we have used this feature to create an in vivo model for real-time analysis of the development, structure and function of the BBB and CP by generating a transgenic zebrafish line that expresses EGFP under the claudin 5a promoter. The high homology and synteny with human, the conservation along the teleost lineage and the previous characterisation of Claudin 5a in zebrafish makes cldn5a a logical candidate (Abdelilah-Seyfried, 2010; Xie et al., 2010; Zhang et al., 2012).. We show that developmental expression of cldn5a:EGFP is restricted to, and starts in both CPs and the midline at 1 dpf, thereby narrowing down the previously shown whole-mount in situ hybridizations (Zhang et al., 2010). The presence of Claudin 5a at the CPs at 1 dpf coincides with the inflation of the ventricles (Zhang et al., 2010, 2012) and corroborates its role in this process. Claudin 5a is crucial ...
We have characterized Tdr1, a family of Tc1-like transposable elements found in the genome of zebrafish (Danio rerio). The copy number and distribution of the sequence in the zebrafish genome have been determined, and by these criteria Tdr1 can be classified as a moderately repetitive, interspersed …
2015. The diets of populations in industrialized nations have shifted to dramatically increased consumption of ω6 polyunsaturated fatty acids (PUFA), with a corresponding decrease in the consumption of ω3 PUFA. This dietary shift may be related to observed increases in obesity, chronic inflammation, and comorbidities in the human population. We examined the effects of ω3:ω6 fatty acid ratios in the context of constant total dietary lipid on the growth, total body fat, and responses of key inflammatory markers in adult zebrafish (Danio rerio). Zebrafish were fed diets in which the ω3:ω6 PUFA ratios were representative of those in a purported ancestral diet (1:2) and more contemporary Western diets (1:5 and 1:8). After 5 mo, weight gain (fat free mass) of zebrafish was highest for those that received the 1:8 ratio treatment, but total body fat was lowest at this ratio. Measured by quantitative real-time RT-PCR, mRNA levels from liver samples of 3 chronic inflammatory response genes ...
The advent of high-throughput sequencing has greatly accelerated the identification of inherited and de novo disease causing mutations. Following discovery the analysis of the developmental and cellular pathways of the affected genes is a crucial step on the path towards therapy with model organisms as the central tool. Traditionally, vertebrate model organisms such as mouse and zebrafish have been used on a gene-by-gene basis, however, in order to keep pace with the increasing speed of discovery, new approaches are needed. Owing to a high quality genome reference sequence and its genetic and embryological tractability the zebrafish is a vertebrate model especially suited for large scale studies. Previously having established methods to generate and identify disruptive zebrafish point mutations on a genome wide scale the Zebrafish Mutation Project is now assigning biological function to every protein-coding gene in the zebrafish genome. We submit alleles to a high-throughput assessment of ...
We used microarray and quantitative real-time PCR (qRT-PCR) analyses in adult female zebrafish (Danio rerio) to identify metabolic pathways regulated by starvation in the liver and brain. The transcriptome of whole zebrafish brain showed little response to 21 days of starvation. Only agouti-related protein 1 (agrp1) significantly responded, with increased expression in brains of starved fish. In contrast, a 21-day period of starvation significantly downregulated 466 and upregulated 108 transcripts in the liver, indicating an overall decrease in metabolic activity, reduced lipid metabolism, protein biosynthesis, proteolysis, and cellular respiration, and increased gluconeogenesis. Starvation also regulated expression of many components of the unfolded protein response, the first such report in a species other than yeast (Saccharomyces cerevisiae) and mice (Mus musculus). The response of the zebrafish hepatic transcriptome to starvation was strikingly similar to that of rainbow trout (Oncorhynchus mykiss) Danio rerio commonly known as zebrafish is a tropical fish belonging to the minnow family (Cyprinidae). It is a major model for studying developmental biology; aided by the transparency of the embryo and adult body, researchers can modify the fishs genotype at the egg stage and see resulting changes as little as three days later.. Zebrafish have the ability to regenerate many of their tissues including fins, skin, heart and, in larval stages, brain. Zebrafish have been used to study cancers, including melanoma, leukemia, pancreatic cancer and hepatocellular carcinoma. The Tubingen strain was used as the the reference strain for the genome sequencing project as it had been used extensively to identify mutations affecting embryogenesis. Sequencing revealed that 71.4% of human genes have at least one zebrafish ortholog. Currently the zebrafish genome is thought to have more than 26,000 protein-coding genes. ...
Because of its permeability to small molecules, zebrafish can be used for testing and screening of drugs affecting different biological processes.50-52 Single and multiple compounds tests can be easily and successfully achieved on both zebrafish embryos and adults.53,54 The reason for this resides in the fact that (1) a high quantity of zebrafish embryos (≈5000) can be obtained synchronously, and (2) the small size of the embryos allows them to fit in a 384-well plate and thus allows high-throughput analyses. In this way, libraries of thousands of compounds can be screened for their effects in a reasonable time frame (eg, weeks). Progresses for drug screening in zebrafish embryos are also subjected to automatic readout for phenotypic effects. An automated high-throughput platform for in vivo chemical screenings on zebrafish embryos has been developed aiming for the highest possible throughput and minimization of human error. It includes an automated method for embryo collection and ...
Non-alcoholic fatty liver disease (NAFLD), characterized as excessive lipid accumulation within hepatocytes, is growing in prevalence. The exploitation of effective drugs for NAFLD has been proven challenging. Herein, we aimed to establish a dietary model of hepatic steatosis using transparent zebrafish larvae in which high-throughput chemical screens could be conducted. Zebrafish larvae fed with high fat (HF) diet and high fat plus high cholesterol (HFC) diet were compared to the control. We analyzed intrahepatic lipid accumulation, biological indexes and various pathways including lipid metabolism, ER stress and inflammation. In addition, the effects of ezetimibe and simvastatin on HFC diet-induced steatosis were evaluated. Zebrafish larvae fed with HF and HFC diets developed steatosis for 7 and 10 days. The incidence and degree of steatosis were more severe in HFC diet-fed larvae compared with the control and HF diet-fed larvae, suggesting that adding cholesterol to the HF diet promotes the hepatic
Won M, Ro H, Dawid IB. Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas. Proc Natl Acad Sci U S A. 2015 Sep
Purpose: Emran et al (2010) reported that the amplitudes of the electroretinograms (ERGs) recorded from isolated eyes of larval zebrafish were normal throughout the day, but were almost absent after several hours of darkness at night. The purpose of our study was to confirm this circadian rhythm of the ERGs in living zebrafish.. Methods: Initially, zebrafish (Danio rerio) were kept in a 14:10 light:dark cycle (room fluorescent light, 9:00 to 23 hours). ERGs were elicited by stimulus intensities of 790 mW/m2 and durations of 1000 msec. The ERGs were recorded with an Ag/AgCl wire electrode that was inserted into a glass micropipette filled with E3 medium. A glasspipette electrode was positioned in the center of the cornea and a reference electrode was an Ag/AgCl pellet that placed beneath the larvae body. The zebrafish was anesthetized by 3-aminobenzoic acid methylester and placed on its side on a moisture sponge with one eye facing toward a light. We recorded ERGs of larval (5 days ...
TY - JOUR. T1 - Dna hypomethylation induces a dna replication-associated cell cycle arrest to block hepatic outgrowth in uhrf1 mutant zebrafish embryos. AU - Jacob, Vinitha. AU - Chernyavskaya, Yelena. AU - Chen, Xintong. AU - Tan, Poh Seng. AU - Kent, Brandon. AU - Hoshida, Yujin. AU - Sadler, Kirsten C.. PY - 2015/2/1. Y1 - 2015/2/1. N2 - UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1) recruits DNMT1 to hemimethylated DNA during replication and is essential for maintaining DNA methylation. uhrf1 mutant zebrafish have global DNA hypomethylation and display embryonic defects, includinga small liver, and theydie as larvae. Wemake the surprising finding that, despite their reduced organ size, uhrf1 mutants express high levels of genes controlling S-phase and have many more cells undergoing DNA replication, as measured by BrdU incorporation. In contrast to wild-type hepatocytes, which are continually dividing during hepatic outgrowth and thus dilute the BrdU label, uhrf1 mutant ...
We provide the first analysis of how a segmentally reiterated pattern of neurons is specified along the anteroposterior axis of the vertebrate spinal cord by investigating how zebrafish primary motoneurons are patterned. Two identified primary motoneuron subtypes, MiP and CaP, occupy distinct locations within the ventral neural tube relative to overlying somites, express different genes and innervate different muscle territories. In all vertebrates examined so far, paraxial mesoderm-derived signals specify distinct motoneuron subpopulations in specific anteroposterior regions of the spinal cord. We show that signals from paraxial mesoderm also control the much finer-grained segmental patterning of zebrafish primary motoneurons. We examined primary motoneuron specification in several zebrafish mutants that have distinct effects on paraxial mesoderm development. Our findings suggest that in the absence of signals from paraxial mesoderm, primary motoneurons have a hybrid identity with respect to ...
Zebrafish (Danio rerio) is a well-recognized model for the study of vertebrate developmental genetics, yet at the same time little is known about the transcriptional events that underlie zebrafish embryogenesis. Here we have employed microarray analysis to study the temporal activity of developmentally regulated genes during zebrafish embryogenesis. Transcriptome analysis at 12 different embryonic time points covering five different developmental stages (maternal, blastula, gastrula, segmentation, and pharyngula) revealed a highly dynamic transcriptional profile. Hierarchical clustering, stage-specific clustering, and algorithms to detect onset and peak of gene expression revealed clearly demarcated transcript clusters with maximum gene activity at distinct developmental stages as well as co-regulated expression of gene groups involved in dedicated functions such as organogenesis. Our study also revealed a previously unidentified cohort of genes that are transcribed prior to the mid-blastula transition,
Purpose: Ca2+ regulates a variety of cellular processes including signal transduction, energy production, and mitochondrial function. Because photoreceptors (PRs) use significant quantities of energy, consuming more ATP/minute per gram tissue than even the brain, our goal is to understand how PRs respond to external fluctuations of Ca2+, and how intracellular Ca2+ dynamics influence energy metabolism. We have previously shown that larval zebrafish PRs display heterogeneous Ca2+ transients. In this study we investigate Ca2+ dynamics in adult zebrafish PRs.. Methods: Adult zebrafish retinas expressing the genetically encoded fluorescent Ca2+ sensor GCaMP in cone PRs were dissected, flat-mounted, and sliced into 400-µm sections. Retinas were stained with propidium iodide or BODIPY and slices were imaged in a temperature-controlled chamber. Retinas were perfused with 0 mM or 3 mM Ca2+ Ringers solution with and without 10 mM isridipine. GCaMP fluorescence was monitored by confocal ...
The genomic organization of the mammalian Pcdh and Ugt1 gene clusters is strikingly similar in that both contain a tandem array of highly similar variable exons followed by a common set of downstream constant exons (Zhang et al., 2004). In contrast to the cell-specific expression of Pcdh genes in the brain, members of the mouse Ugt1 cluster display tissue-specific expression patterns in a wide variety of tissues, especially in the digestive and respiratory systems (Zhang et al., 2004). In addition, the variable and constant genomic organizations of both the Pcdh and Ugt1 clusters are conserved in zebrafish (Wu, 2005; Li and Wu, 2007). The zebrafish has become an important vertebrate model organism for developmental biology, physiology, and pathology studies (Howe et al., 2013). Recently, there has been great interest in developing the zebrafish as a vertebrate model organism for drug discovery as well as for environmental toxicology (Hill et al., 2005; Peterson and Macrae, 2012). The full ...
We recently identified Rbm24 as a novel gene expressed during mouse cardiac development. Due to its tightly restricted and persistent expression from formation of the cardiac crescent onwards and later in forming vasculature we posited it to be a key player in cardiogenesis with additional roles in vasculogenesis and angiogenesis. To determine the role of this gene in cardiac development, we have identified its zebrafish orthologs (rbm24a and rbm24b), and functionally evaluated them during zebrafish embryogenesis. Consistent with our underlying hypothesis, reduction in expression of either ortholog through injection of morpholino antisense oligonucleotides results in cardiogenic defects including cardiac looping and reduced circulation, leading to increasing pericardial edema over time. Additionally, morphant embryos for either ortholog display incompletely overlapping defects in the forming vasculature of the dorsal aorta (DA), posterior caudal vein (PCV) and caudal vein (CV) which are the first blood
In the past decade, the zebrafish, Danio rerio, has risen to much greater prominence as a vertebrate model system for drug discovery and toxicity testing. Zebrafish larvae represent an in vivo vertebrate model, with high throughput potential and proven efficacy as a model of human disease and drug responses. Specifically, zebrafish have been used for cardiotoxicity studies and cardiac arrhythmia modelling.. This project aims to develop and optimise tools for electrocardiographic recording in zebrafish larvae for use in cardiotoxicity screening and human arrhythmia modelling. Steps were taken towards a high throughput ECG system for zebrafish larvae through establishing viability of non-contact capillary electrode recording and development of microfabricated electrode arrays capable of replacing glass capillary electrodes. In depth analysis of atrium and ventricle signals using wavelet analysis was performed and the frequency profiles for these chambers examined. The utility of the larval ECG ...
The genetic pathways underlying the induction and anterior-posterior patterning of the heart are poorly understood. The recent emergence of the zebrafish model system now allows a classical genetic approach to such challenging problems in vertebrate development. Two large-scale screens for mutations affecting zebrafish embryonic development have recently been completed; among the hundreds of mutations identified were several that affect specific aspects of cardiac morphogenesis, differentiation, and function. However, very few mutations affecting induction and/or anterior-posterior patterning of the heart were identified. We hypothesize that a directed approach utilizing molecular markers to examine these particular steps of heart development will uncover additional such mutations. To test this hypothesis, we are conducting two parallel screens for mutations that affect either the induction or the anterior-posterior patterning of the zebrafish heart. As an indicator of cardiac induction, we ...
Had gathered much info from this site, so thought I should contribute my experience. Im new to breeding Danios, all by accident really. Within a month of setting up my 5ft tank, I had very busy zebra danios chasing each other. A few days later I did a gravel vacuum, and emptied the water into the bath, letting it go down the drain. On the 4th bucket I decided to put the plug in, and discovered babies in the bucket. (Who knows how many I sent down the drain...)We moved them to the main tank, in a floating fry tank covered with an ankle stocking around it for finer holes. Left this to float on the top. Discovered that the water surface doesnt get agitated enough in these and babies died off. When I let fresh water in the top daily, the fish were much happier. Did another gravel vac 2 weeks later, and found more fry, so suspected theyd had another batch. My Leopard Danio girl got very plump, so I moved her and her mate into a small tank for spawning one night, and the next morning she was very ...
Basic research in pattern formation is concerned with the generation of phenotypes and tissues. It can therefore lead to new tools for medical research. These include phenotypic screening assays, applications in tissue engineering, as well as general advances in biomedical knowledge. Our aim here is to discuss this emerging field with special reference to tools based on zebrafish developmental biology. We describe phenotypic screening assays being developed in our own and other labs. Our assays involve: (i) systemic or local administration of a test compound or drug to zebrafish in vivo; (ii) the subsequent detection or readout of a defined phenotypic change. A positive readout may result from binding of the test compound to a molecular target involved in a developmental pathway. We present preliminary data on assays for compounds that modulate skeletal patterning, bone turnover, immune responses, inflammation and early-life stress. The assays use live zebrafish embryos and larvae as well as adult
a. SOX10/MyoD in situ experiments on zebrafish colec11 and masp1 morphants showing an abnormal distribution of the CNCC in the hindbrain of 10 somite-stage embr
The easy-to-use in vivo model, zebrafish larva, is being increasingly used to screen chemical-induced hepatotoxicity, with a good predictivity for various mechanisms of liver injury. However, nothing is known about its applicability in exploring the mechanism called membrane remodeling, depicted as changes in membrane fluidity or lipid raft properties. The aim of this study was, therefore, to substantiate the zebrafish larva as a suitable in vivo model in this context. Ethanol was chosen as a prototype toxicant because it is largely described, both in hepatocyte cultures and in rodents, as capable of inducing a membrane remodeling leading to hepatocyte death and liver injury. The zebrafish larva model was demonstrated to be fully relevant as membrane remodeling was maintained even after a 1-week exposure without any adaptation as usually reported in rodents and hepatocyte cultures. It was also proven to exhibit a high sensitivity as it discriminated various levels of cytotoxicity depending on the extent
Autoimmunity exhibits a female bias which is consistent across many disorders including multiple sclerosis (MS), systemic lupus erythematous (SLE), and rheumatoid arthritis. However, much of the interplay between environment, sex and autoimmunity is not understood. FoxP3 is the primary transcription factor controlling the development of regulatory T cells (Tregs) which help control the immune system through suppressive effects. This study aims to evaluate how an increase in dietary sodium affects the expression of zFoxP3. Additionally, this study examines if zFoxP3is differentially expressed between males and females. Zebrafish were separated into test and control groups with each group having equal numbers of male and female fish. Following an acclimation period, the diet of the test fish was modified to include a ten-times increase in sodium concentration. Three male and three female zebrafish from each group were sampled at 1, 2, and 4 weeks following the introduction of the increased sodium ...
Autoimmunity exhibits a female bias which is consistent across many disorders including multiple sclerosis (MS), systemic lupus erythematous (SLE), and rheumatoid arthritis. However, much of the interplay between environment, sex and autoimmunity is not understood. FoxP3 is the primary transcription factor controlling the development of regulatory T cells (Tregs) which help control the immune system through suppressive effects. This study aims to evaluate how an increase in dietary sodium affects the expression of zFoxP3. Additionally, this study examines if zFoxP3is differentially expressed between males and females. Zebrafish were separated into test and control groups with each group having equal numbers of male and female fish. Following an acclimation period, the diet of the test fish was modified to include a ten-times increase in sodium concentration. Three male and three female zebrafish from each group were sampled at 1, 2, and 4 weeks following the introduction of the increased sodium ...
Zebrafish neurogenesis (nerve-cell creation): Left: zebrafish brain showing microglia cells. Center: lesion (damage) results in microglia activation and
While such recovery from biological limbo seemingly defies scientific or medical explanation, researchers at Seattles Fred Hutchinson Cancer Research Center have developed a method to induce a similar state of so-called suspended animation in the zebrafish, a relatively new model of vertebrate developmental biology.
The underlying processes of nociception and pain are, despite the rodent models available, still not fully understood. One of the drawbacks of rodent model systems is the difficulty to screen compound libraries for their influence on nociception, thus slowing down the discovery of novel analgesics for clinical use.. Rodent behavioural tasks have been previously adapted for larval zebrafish in our group and in the current manuscript we investigated the possibilities of zebrafish larvae as an additional model system to study nociception and pain and their underlying mechanisms.. Zebrafish larvae were exposed to different concentrations of diluted acetic acid, a chemical noxious stimulus, and we measured nociceptive-specific behaviours. Cyclooxygenase-2 (cox-2), a gene known to be involved in nociception, was used as a marker for the activation of nociceptive pathways. Upon exposure to diluted acetic acid, five-day old larval zebrafish showed a concentration dependent increase in locomotor ...
Genomes and Genes, Publications, Species, Locale, Scientific Experts, Research Topics about Experts and Doctors on zebrafish in Iowa City, Iowa, United States
My lab uses zebrafish as a model to study myelination in the development central nervous system. The small size, optical transparency, relative simplicity, and rapid development of zebrafish embryos are properties that allow direct observation of entire developmental (or repair) events as they occur in live animals. We have developed a non-invasive transgenic method to induce demyelination in zebrafish and have also generated a suite of tools to visualise myelin and myelinated axons at high-resolution in live zebrafish, which allows us to observe cellular, sub-cellular and molecular behaviours during myelination, demyelination, and remyelination as they occur in a living animal. Zebrafish are well established as a powerful system with which to identify new genes required for biological events. In a genetic screen carried out at Stanford University I identified new roles for genes with known involvement in myelination, established zebrafish models of human disease, and identified new genes ...
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The zebrafish model organism database (ZFIN) provides a Web resource of zebrafish genomic, genetic, developmental, and phenotypic data
Insects represent a valuable and sustainable alternative ingredient for aquafeed formulation. However, insect-based diets have often highlighted controversial results in different fish species, especially when high inclusion levels were used. Several studies have demonstrated that nutritional programming through parental feeding may allow the production of fish better adapted to use sub-optimal aquafeed ingredients. To date, this approach has never been explored on insect-based diets. In the present study, five experimental diets characterized by increasing fish meal substitution levels with full-fat Black Soldier Fly (Hermetia illucens; BSF) prepupae meal (0%, 25%, 50%, 75% and 100%) were used to investigate the effects of programming via broodstock nutrition on F1 zebrafish larvae development. The responses of offspring were assayed through biometric, gas chromatographic, histological, and molecular analyses. The results evidenced that the same BSF-based diets provided to adults were able to ...
The zebrafish, Danio rerio, has become a popular vertebrate model for the study of infections, mainly because of its excellent optical accessibility at the
Due to the important biological role of red blood cells (RBCs) in vertebrates, the analysis of reshaping and dynamics of RBCs motion is a critical issue in physiology and biomechanics. In this paper the behavior of RBCs within the immature capillary plexus during embryonic development of zebrafish has been analyzed. Relying on the fact that zebrafish embryos are small and optically transparent, it is possible to image the blood flow.. In this way the anatomy of blood vessels is monitored along with the circulation throughout their development. Numerical simulations were performed using a specific numerical model that combines fluid flow simulation, modeling of interaction of individual red blood cells immersed in blood plasma with the surrounding fluid and modeling the deformation of individual cells. The results of numerical simulations are in accordance with the in vivo observed region of interest within the caudal vein plexus of the zebrafish embryo. Good agreement of results demonstrates the
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We investigated the effects of short-term exposure of bare TiO2NPs and polyelectrolyte-coated TiO2NPs in the 5-25 nm size range, at relatively high concentrations (of 500 and 1000 mg/L) under light or dark conditions, in D. rerio embryos. The biological endpoints investigated included embryo viability and mRNA transcript levels of antioxidant and membrane transport genes relative to control embryos. The presence of nanoparticles on the surface of embryos was assessed using TEM. The results confirm an accumulation of TiO2NPs on the outer surface (chorion) of the embryo, but not within the embryo. No significant difference in embryo viability was detected following each exposure regime. The expression of antioxidant biomarker, SOD2, was significantly impacted by the type of TiO2NP, with TiO2NPs/PSS/PAH coating exposure showing down regulation; the concentration of the nanoparticles, with down regulation at 500 mg/L; and dark/light condition with down regulation in the light. The expression levels ...
Collective migration of cells in the zebrafish Posterior Lateral Line primordium (PLLp) along a path defined by Cxcl12a expression depends on Cxcr4b receptors in leading cells and on Cxcr7b in trailing cells. Cxcr7b-mediated degradation of Cxcl12a by trailing cells generates a local gradient of Cxcl12a that guides PLLp migration. Agent-based computer models were built to explore how a polarized response to Cxcl12a, mediated by Cxcr4b in leading cells and prevented by Cxcr7b in trailing cells, determines unidirectional migration of the PLLp. These chemokine signaling-based models effectively recapitulate many behaviors of the PLLp and provide potential explanations for the characteristic behaviors that emerge when the PLLp is severed by laser to generate leading and trailing fragments. As predicted by our models, the bilateral stretching of the leading fragment is lost when chemokine signaling is blocked in the PLLp. However, movement of the trailing fragment toward the leading cells, which was ...
We investigated the mechanisms of intermediate cell mass (ICM) expansion in zebrafish chordin (Chd) morphant embryos and examined the role of BMPs in relation to this phenotype. At 24 h post-fertilization (hpf), the expanded ICM of embryos injected with chd morpholino (MO) (Chd MO embryos) contained a monotonous population of hematopoietic progenitors. In situ hybridization showed that hematopoietic transcription factors were ubiquitously expressed in the ICM whereas vascular gene expression was confined to the periphery. BMP4 (but not BMP2b or 7) and smad5 mRNA were ectopically expressed in the Chd MO ICM. At 48 hpf, monocytic cells were evident in both the ICM and circulation of Chd MO but not WT embryos. While injection of BMP4 MO had no effect on WT hematopoiesis, co-injecting BMP4 with chd MOs significantly reduced ICM expansion. Microarray studies revealed a number of genes that were differentially expressed in Chd MO and WT embryos and their roles in hematopoiesis has yet to be ...
One of the fundamental problems in developmental biology is understanding how the behaviour of single cells affects global tissue and organ morphogenesis. The development of the zebrafish posterior lateral line primordium (PLLP) provides an excellent system for the study of dynamic single cell behaviours that contribute to higher order tissue organization (Ghysen and Dambly-Chaudière, 2007). The lateral line is a sensory system found in fish and amphibians that is dedicated to the detection of water movements. These movements are registered by specialized sense organs, the neuromasts, which consist of a central mechanosensory hair cell bundle and surrounding support cells. The lateral line system itself can be divided into two parts: an anterior one, which comprises the neuromasts of the head; and a posterior one, which comprises the neuromasts of the body and tail. Most of the research in the field has focused on the development of the posterior lateral line (PLL), owing to its stereotypy and ...
Despite the well-characterized occurrence of uranium (U) in the aquatic environment, very little is known about the chronic exposure of fish to low levels of U and its potential effect on reproduction. Therefore, this study was undertaken to investigate the effects of environmental concentrations of depleted U on the reproductive output of zebrafish (Danio rerio) and on survival and development of the F1 embryo-larvae following parental exposure to U. For that purpose, sexually mature male and female zebrafish were exposed to 20 and 250 µg/L of U for 14 days and allowed to reproduce in clean water during a further 14-day period. At all sampling times, whole-body vitellogenin concentrations and gonad histology were analyzed to investigate the effects of U exposure on these reproductive endpoints. In addition, accumulation of U in the gonads and its genotoxic effect on male and female gonad cells were quantified. The results showed that U strongly affected the capability of fish to reproduce and to
Currently, there is no other whole-animal reporter for epigenetic regulation established in any vertebrate. The inventors generated this novel zebrafish line using a transgene construct containing dazl gene silencing sequences (CpG island) fused to a destabilized GFPd2 gene driven by the ubiquitously expressing ef1alpha promoter. The resulting transgenic line permits detailed tissue- or cellular- level visualization of dynamic changes in GFPd2 expression in response to changes in DNA methylation or downstream epigenetic regulation in developing or adult animals. GFPd2 is off in the fertilized egg but turns on during early development, peaking at approximately 24 hours post-fertilization, and is then rapidly and ubiquitously silenced. The reporter is off in adults, except in particular stages of germline development in the gonads. Experimental treatments or genetic mutants that interfere with epigenetic silencing result in global or tissue-specific reactivation of GFPd2 expression. The ...
Crude oil spills have caused substantial impacts to aquatic ecosystems. Chemical dispersants are used to palliate the impact of oil spillages, but their use is polemic due to their additional potential toxic effect when mixed with oil-derived components. In this work, we used a 16S-based metagenomic approach to analyze the changes of the gut microbiota of adult zebrafish (Danio rerio) exposed to the water accommodated fraction (WAF) of a light crude oil (35° API gravity), and the chemically enhanced WAF (CEWAF), prepared with Nokomis 3-F4® dispersant. After 96 h of exposure, WAF induced an increase in the alpha and beta diversity, altering the relative abundance of Vibrio, Flavobacterium, and Novosphingobium. In contrast, CEWAF only caused an increase in the beta diversity, and an enrichment of the genus Pseudomona. Both treatments diminished the abundances of Aeromonas, Cetobacterium, Coxiella, Dinghuibacter, and Paucibacter. Moreover, the co-occurrence network among genera was more complex ...
Article Exposure to a PBDE/OH‐BDE mixture alters juvenile zebrafish (Danio rerio) development. Polybrominated diphenyl ethers (PBDEs) and their metabolites (e.g. hydroxylated BDEs (OH‐BDEs)) are contaminants detected together frequently in human tiss...
It has been widely supported that individual animals express different strategies to cope with environmental challenge. In ectothermic species such as fish, individuals must use behavioral thermoregulation mechanisms to optimize physiological performance. In the present study, thermal preference was tested in groups of wild-type zebrafish, Danio rerio, screened for proactive and reactive animal personalities. Three replicate groups of proactive, reactive, and naive randomly sampled non-screened controls were used for the experiments. The frequency distribution of the animals was recorded in a custom-built multichamber tank under both constant temperature (temperature restricted conditions: TR) and a continuous thermal gradient profile (temperature choice: TCh ranging from 21°C to 35°C). Proactive and reactive animal personalities expressed significantly different thermal preferences and general activity within the temperature gradient. Our results show that proactive fish, generally ...
Effects of 3,4-dichloroaniline and diazinon on different biological organisation levels of zebrafish (Danio rerio) embryos and ...
Effects of the Protein Kinase Inhibitor PKC412 on Gene Expression and Link to Physiological Effects in Zebrafish Danio rerio Eleuthero- ...
This dissertation describes research to elucidate the early steps in the process of synapse formation in the zebrafish spinal cord. One question is how presynaptic proteins are trafficked and recruited to nascent synapses. Previous work has suggested two possible models of presynaptic transport, either (1) most presynaptic proteins are transported together or (2) two types of transport packets, synaptic vesicle (SV) protein transport vesicles (STVs) and Piccolo-containing active zone precursor transport vesicles (PTVs), transport the necessary components separately. We tested these models using in vivo imaging in zebrafish spinal cord and found that the recruitment of at least three distinct transport packets during presynaptic assembly of a glutamatergic synapse occurs in an ordered sequence. First, STVs are stabilized at future synaptic sites, then PTVs, followed by a third transport packet type carrying Synapsin, a cytosolic protein that can tether SVs to actin. These results identify an ...
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The zebrafish posterior lateral line (pLL) is a sensory system that comprises clusters of mechanosensory organs called neuromasts (NMs) that are stereotypically positioned along the top of trunk. occasions that are controlled from the Wnt pathway arent known. We determined a mutant stress mutants absence posterior NMs and live imaging reveals that rosette renewal fails during later on phases of migration. Remarkably NVP-ADW742 the entire primordium patterning as assayed from the expression of varied markers shows up unaltered in mutants. Lineage tracing and mosaic analyses exposed how the leading cells (presumptive progenitors) re-locate from the primordium and so are integrated into NMs; this total leads to a reduction in the amount of proliferating progenitor cells and eventual primordium disorganization. We figured Lef1 function is not needed for preliminary primordium corporation or migration but is essential for proto-NM renewal during later on phases of pLL development. These findings ...
Macquarie University researchers have developed the first zebrafish model of the neurodegenerative Machado-Joseph Disease - and have used this model to test drugs that could potentially be used to treat the disease, which disproportionately affects Indigenous Australians.. Machado-Joseph Disease, or spinocerebellar ataxia-3, is a hereditary disease caused by a gene mutation, which leads to a progressive loss of muscle control and movement. Most people living with MJD are wheelchair bound and need constant support within 15 years of symptoms first emerging.. Researchers gave the zebrafish the human disease-causing gene, creating the first transgenic zebrafish model of MJD, allowing them to compare the movement, pathology and lifespan of fish carrying the healthy version of the human gene to those with the disease-causing version.. Zebrafish are small and transparent, allowing us to see how a disease develops. Being able to use zebrafish to model MJD is a great win for those of us working to ...
Sexually dimorphic breeding tubercles (BTs) are keratinized epidermal structures that form clusters on the dorsal surface of the anterior rays of zebrafish male pectoral fins. BTs appear during sexual maturation and are maintained through regular shedding and renewal of the keratinized surface. Following pectoral fin amputation, BT clusters regenerate after the initiation of revascularization, but concomitantly with a second wave of angiogenesis. This second wave of regeneration forms a web-like blood vessel network that penetrates the supportive epidermis of BTs. Upon analyzing the effects of sex steroids and their inhibitors, we show that androgens induce and estrogens inhibit BT cluster formation in intact and regenerating pectoral fins. Androgen-induced BT formation in females is accompanied by the formation of a male-like blood vessel network. Treatment of females with both androgens and an angiogenesis inhibitor results in the formation of undersized BT clusters when compared with females ...
LPA signaling plays important roles during cell migration and proliferation in normal and pathological conditions. However, its role during sensory organ development remains unknown. Here we show a LPA receptor Lpar2b is expressed in the posterior lateral line primordium (pLLP) and mechanosensory organs called neuromasts (NMs) in zebrafish embryos. Lpar2b loss-of-function significantly reduces the number of NMs and hair cells in the posterior lateral line (pLL). Further analysis reveals that Lpar2b regulates the patterning and organ size of the pLLP. Interestingly, we show that knocking down a Hippo effector Yap1 phenocopies the result of Lpar2b depletion, and Lpar2b regulates the phosphorylation and activity of Yap1 in the pLLP. Importantly, a phosphorylation-resistant Yap1 rescues pLLP organ size and NM number in Lpar2b-depleted embryos. Our results indicate Lpar2b controls the sensory organ size and NM number by regulating Yap1 activity in the lateral line system.
Because Ras signaling is frequently activated by major hepatocellular carcinoma etiological factors, a transgenic zebrafish constitutively expressing the krasV12 oncogene in the liver was previously generated by our laboratory. Although this model depicted and uncovered the conservation between zebrafish and human liver tumorigenesis, the low tumor incidence and early mortality limit its use for further studies of tumor progression and inhibition. Here, we employed a mifepristone-inducible transgenic system to achieve inducible krasV12 expression in the liver. The system consisted of two transgenic lines: the liver-driver line had a liver-specific fabp10 promoter to produce the LexPR chimeric transactivator, and the Ras-effector line contained a LexA-binding site to control EGFP-krasV12 expression. In double-transgenic zebrafish (driver-effector) embryos and adults, we demonstrated mifepristone-inducible EGFP-krasV12 expression in the liver. Robust and homogeneous liver tumors developed in 100% ...
Prenatal propofol exposure induced neurotoxicity in the developing brains and led to persistent learning deficits in the offspring. Our goal was to use zebrafish to explore whether the decline in learning and memory was correlated with inhibition of neuronal growth after propofol exposure. Zebrafish embryos at 6 hours postfertilization (hpf) were exposed to control or 1, 2 or 4 μg/mL propofol until 48 hpf. Spontaneous locomotor activity and swimming behavior in response to dark‐to‐light photoperiod stimulation were studied in zebrafish larvae at 6 days postfertilization (dpf). The adaptability to repeated stimulation was used to indicate learning and memory function of larvae. Transgenic NBT line zebrafish was used to quantitate the effect of propofol on motor neuronal growth of embryos in vivo. Six dpf transgenic zebrafish larvae went through photoperiod stimulation after their neuronal length had been analyzed during the embryonic period. Our data indicate that embryonic exposure to 1, 2 ...
Synthetic glucocorticoids (GCs) are potential endocrine disrupting compounds that have been detected in the aquatic environment around the world in the low ng/L (nanomolar) range. GCs are used as immunosuppressants in medicine. It is of high interest whether clobetasol propionate (CP), a highly potent GC, suppresses the inflammatory response in fish after exposure to environmentally relevant concentrations. Bacterial lipopolysaccharide (LPS) challenge was used to induce inflammation and thus mimic pathogen infection. Zebrafish embryos were exposed to ≤1000 nM CP from ~1 h post fertilization (hpf) to 96 hpf, and CP uptake, survival after LPS challenge, and expression of inflammation-related genes were examined. Our initial experiments were carried out using 0.001% DMSO as a solvent vehicle, but we observed that DMSO interfered with the LPS challenge assay, and thus masked the effects of CP. Therefore, DMSO was not used in the subsequent experiments. The internal CP concentration was ...
TY - JOUR. T1 - Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size. AU - Guo, Cuicui. AU - Chen, Xia. AU - Song, Huaidong. AU - Maynard, Michelle A.. AU - Zhou, Yi. AU - Lobanov, Alexei V.. AU - Gladyshev, Vadim N.. AU - Ganis, Jared J.. AU - Wiley, David. AU - Jugo, Rebecca H.. AU - Lee, Nicholas Y.. AU - Castroneves, Luciana A.. AU - Zon, Leonard I.. AU - Scanlan, Thomas S.. AU - Feldman, Henry A.. AU - Huang, Stephen A.. PY - 2014/10/1. Y1 - 2014/10/1. N2 - Thyroid hormone is a master regulator of differentiation and growth, and its action is terminated by the enzymatic removal of an inner-ring iodine catalyzed by the selenoenzyme type 3 deiodinase (dio3). Our studies of the zebrafish reveal that the dio3 gene is duplicated in this species and that embryonic deiodination is an important determinant of embryo size. Although both dio3 paralogs encode enzymatically active proteins with high affinity for thyroid hormones, their anatomic patterns of ...
Most human birth defects are highly variable. Our ability to diagnose, treat and prevent defects relies on our understanding of this variability. Mutation of the transcription factor GATA3 in humans causes the highly variable Hypoparathyroidism, sensorineural Deafness and Renal dysplasia (HDR) syndrome. Although named for a triad of defects patients with HDR can also exhibit craniofacial defects. Through a forward genetic screen for craniofacial mutants, we isolated a zebrafish mutant in which the first cysteine of the second zinc finger of Gata3 is mutated. Because mutation of the homolgous cysteine causes HDR in humans, these zebrafish mutants will be a rapid and effective animal model for understanding the role of gata3 in the HDR disease spectrum. We demonstrate that, unexpectedly, the chaperone proteins Ahsa1 and Hsp90 promote severe craniofacial phenotypes in our zebrafish model of HDR Syndrome. The strengths of the zebrafish system, including rapid development, genetic tractability and ...
Specifically, qRT-PCR analysis of gene expression in living zebrafish embryos was applied to detect and characterize the effects of: (1) EDCs that act via ERs to induce brain P450 aromatase (P450aromB) and hepatic vitellogenin (vtg) expression; (2) EDCs that act via AhRs to reduce gonadal aromatase (P450aromA) and increase hepatic P4501A1 expression; (3) EDCs that interact directly with preformed aromatase enzymes to block aromatization; and (4) EDCs that perturb ER and AhR expression.. Primer Design and Optimization of qRT-PCR Conditions. P450aromB and -A. Assay development and validation in embryonic, larval, and adult male and female zebrafish has been completed (Sawyer and Callard, in preparation, 2004). Results show that the assay is sensitive and precise (linear between 1 x 10-6 and 1.0 ng mRNA). Reproducibility is excellent within and across operators when tested in multiple experiments and at multiple starting points within a single experiment (e.g., RNA extraction, RT, PCR)(intra- and ...
Despite detailed in vivo knowledge of glycolytic enolases and many bacterial non-enolase members of the superfamily, little is known about the in vivo function of vertebrate non-enolase enolase superfamily members (ENOSF1s). Results of previous studies suggest involvement of the β splice form of ENOSF1 in breast and colon cancers. This study used the zebrafish (Danio rerio) as a vertebrate model of ENOSF1β function. Whole mount in situ hybridization (WISH) showed that zebrafish ENOSF1β (enosf1b) is zygotic and expressed ubiquitously through the first 24 hours post fertilization (hpf). After 24 hpf, enosf1b expression is restricted to the notochord. Embryos injected with enosf1b-EGFP mRNA grew slower than EGFP mRNA-injected embryos but caught up to the EGFP-injected embryos by 48 hpf. Embryos injected with ATG or exon 10 enosf1b mRNA-targeting morpholinos had kinked notochords, shortened anterior-posterior axes, and circulatory edema. WISH for ntl or pax2a expression showed that embryos injected with
Using a 3D deformable cell-based simulation, coupled to reaction-diffusion PDEs for chemical concentrations, we model and simulate the polarization, migration, and neuromast deposition in the zebrafish posterior lateral line primordium (PLLP). This primordium migrates from head to tail of the fish embryo, and deposits sensory neuromasts at regular intervals in its wake. The model combines realistic cell-cell adhesion, chemotaxis and interaction forces to describe the emergence of tissue polarity, as well as the cell-cluster shape and speed of collective cell migration. We base the chemical signalling model on receptor-ligand kinetics and mutually inhibitory FGF-Wnt signalling. Our signalling submodel allows predictions to be made for the position of the front-rear boundary in the PLLP, as a function of aggregate parameters such as steady-state receptor expression levels and IC50 parameters for the mutually-inhibitory effect. Our 3D model allows an investigation of the role of cell division, ...
5G Networks Health Impact Is Predominately Benign: New Analysis From Oregon State On Zebrafish Finds That 5G Likely Not Harmful, read now.
J Vis Exp. 2012 Dec 16;(70). pii: 4243. doi: 10.3791/4243. Chang JT, Sive H. Source Department of Biology, Whitehead Institute of Biomedical Research, Massachusetts Institute of Technology. Abstract Cerebrospinal fluid (CSF) is a protein rich fluid contained within the brain ventricles. It is present during early vertebrate embryonic development and persists throughout life. Adult CSF is thought to cushion the brain, remove waste, and carry secreted molecules(1,2). In the adult and older embryo, the majority of CSF is made by the choroid plexus, a series of highly vascularized secretory regions located adjacent to the brain ventricles(3-5). In zebrafish, the choroid plexus is fully formed at 144 hours post fertilization (hpf)(6). Prior to this, in both zebrafish and other vertebrate embryos including mouse, a significant amount of embryonic CSF (eCSF) is present . These data and studies in chick suggest that the neuroepithelium is secretory early in development and may be the major source of ...
Modeling signaling and migration of the Posterior Lateral Line primordium with agent based models Formation of the Posterior Lateral Line (PLL) is spearheaded b...
etv5a expression was analyzed by whole-mount in situ hybridization and quantitative real-time PCR of zebrafish embryos at developmental stages ranging from one-cell stage to 48 hours post fertilization (hpf). A strong and ubiquitous signal was detected from one-cell to the oblong stage, which indicated that etv5a was expressed as a maternal mRNA (Fig. 1A-C). Ubiquitous expression was observed throughout the whole embryo before gastrulation (Fig. 1D,E). During early gastrulation (75% epiboly), expression localized to the ventral mesoderm (arrowhead in Fig. 1F,G). At later stages, etv5a expression became confined to the lateral mesoderm as demonstrated by the presence of two longitudinal stripes at the six-somite stage (Fig. 1H-J). A previous study showed that cells present in this region are multipotent progenitors that give rise to hematopoietic, endothelial and pronephric derivatives (Gering et al., 1998). etv5a expression was maintained in the posterior lateral mesoderm (Fig. 1K-N) during ...
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The use of nicotine, a known anxiolytic with addictive properties, is prevalent; however, the effects on the brain are not fully elucidated. Previous studies investigating the effects of nicotine on the brain have been limited to developmental models. In this two-part study, we investigated the effect of nicotine on protein kinase B (AKT) and glycogen synthase kinase-3β (GSK-3β) in the brain of adult zebrafish. AKT plays a role in neuronal growth, plasticity, and metabolism. AKT is activated via phosphorylation on Ser473 or Thr308 by growth factors and increased neuronal activity. AKT regulates constitutively active GSK-3β via phosphorylation on Ser9. This is important in neurophysiology, because unregulated GSK-3β is associated with cell death, dysregulated metabolic function, and neuro-inflammation. Two protocols were used for measuring the effect of nicotine on the adult zebrafish brain: an in-vivo (immersion) treatment, and an ex-vivo treatment. The protocol for immersion experiment included
2-Methoxyestradiol (2ME), an endogenous metabolite of 17β-estradiol, has been previously reported to possess antiangiogenic and antitumor properties. Herein, we demonstrate that the effects of this antiangiogenic steroid can be readily assayed in live zebrafish, introducing a convenient and robust new model system as a screening tool for both single cell and collective cell migration assays. Using the in vitro mammalian endothelial cell line EA.hy926, we first show that cell migration and angiogenesis, as estimated by wound assay and tube formation respectively, are antagonized by 2ME. In zebrafish (Danio rerio) larvae, dose-dependent exposure to 2ME diminishes (1) larval angiogenesis, (2) leukocyte recruitment to damaged lateral line neuromasts and (3) retards the lateral line primordium in its migration along the body. Our results indicate that 2ME has an effect on collective cell migration in vivo as well as previously reported anti-tumorigenic activity and suggests that the molecular mechanisms
Interest in using zebrafish is on the rise for several reasons. Zebrafish are small and inexpensive to maintain, and they produce hundreds of offspring in every mating, easily accumulating statistically significant numbers of animals. Whats more, zebrafish embryos are transparent and develop outside the uterus, making it easy to monitor developing fish. In fact, zebrafish embryos are tiny enough to be raised in microplate wells for high-throughput whole-animal assays. Many test compounds can diffuse into zebrafish embryos, simplifying drug administration. Researchers can also readily identify zebrafish with gene mutations. Lastly, as vertebrates, zebrafish and other fish have most of the same organs and organ systems that mammals do, making them more relevant in whole-animal assays than fruit flies or roundworms, which have also been used in early drug discovery screening.. ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Wild-type zebrafish (Danio rerio) are characterized by black and yellow stripes, which form on their body and fins due to the self-organization of thousands of pigment cells. Mutant zebrafish and sibling… ...
Avoiding temperatures outside the physiological range is critical for animal survival, but how temperature dynamics are transformed into behavioral output is largely not understood. Here, we used an infrared laser to challenge freely swimming larval zebrafish with white-noise heat stimuli and built quantitative models relating external sensory information and internal state to behavioral output. These models revealed that larval zebrafish integrate temperature information over a time-window of 400 ms preceding a swimbout and that swimming is suppressed right after the end of a bout. Our results suggest that larval zebrafish compute both an integral and a derivative across heat in time to guide their next movement. Our models put important constraints on the type of computations that occur in the nervous system and reveal principles of how somatosensory temperature information is processed to guide behavioral decisions such as sensitivity to both absolute levels and changes in stimulation ...
Danio rerio (zebrafish) have rapidly gained popularity in the study of vertebrate development and gene function. Zebrafish are an excellent model for performing large-scale genetic and drug screens to be performed coupled with the visualization of downstream perturbations on live, developing organisms. Due to the fact that large numbers of embryos are required to perform these experiments, we investigated ways to efficiently increase zebrafish embryo production while not being overly invasive or using chemicals that might affect the developmental processes. To perform these experiments, we mated wild type (wt) zebrafish while exposing them to the different wavelengths of light. Fish were exposed to 12 h of dark, followed by 4 h with the different colored light treatments. Once this light cycle was over, embryo production was enumerated. Results indicated that exposing zebrafish to the different wavelengths of fluorescent and LED light had little effect on embryo production. Further research may
The bromophenol 2,4,6-tribromophenol (TBP) is widely used as an industrial chemical, formed by degradation of tetrabromobisphenol-A, and it occurs naturally in marine organisms. Concentrations of TBP in fish have been related to intake via feed, but little is known about effects on fish health after oral exposure. In this study, we exposed adult male and female zebrafish (Danio rerio) to TBP via feed in nominal concentrations of 33, 330, and 3300 μg/g feed (or control feed) for 6 weeks to assess the effects of TBP on reproductive output, gonad morphology, circulatory vitellogenin levels, and early embryo development. The aim was also to investigate the extent to which TBP was metabolised to 2,4,6-tribromoanisole (TBA) in dietary exposed zebrafish, and the amounts of TBP and TBA found in offspring. After 6 weeks of exposure, we found about 3% of the daily dose of TBP in adult fish and the mean concentration of TBA was 25-30% of the TBP concentration. TBP and TBA were detected in offspring with ...
and Characterization of zebrafish von Willebrand factor reveals conservation of domain structure, multimerization, and intracellular storage by J. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. Maintaining the Barrier Function of Blood Vessels. I. Zebrafish are a promising model for obesity research, as lipid metabolism pathways are conserved between mammals and fish [44-46].Zebrafish have the key organs that are important for energy homeostasis and metabolism in mammals, as well as other key functions such as appetite regulation in the brain [], insulin regulation [], endocrine signaling through leptin [], and … The present review provides a brief overview of recent studies on wild‐type and transgenic zebrafish as a model system to monitor toxic heavy metals, endocrine disruptors, and organic pollutants for toxicology. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, ...
The epithelium is the outer most layer of cells responsible for mediating the interactions of an organism with its external environment. Squamous cells are scale-like flattened cells that form the most superficial layer of the epithelium. Regulatory errors in these cells can lead to squamous cell carcinoma, the second most common form of skin cancer. Understanding of the underlying molecular mechanisms is integral for improved diagnostics and therapeutics. IKK1 is a known regulator of several important pathways including regulation of epidermal differentiation and proliferation. Changes in IKK1 expression have been linked to tumor proliferation in squamous cell carcinomas. IKK1 -/- knockout mice have proven critical in understanding the function of IKK1 but are difficult in studies of early epidermal development. The novel zebrafish maternal-effect mutant poky contains a mutation in the zebrafish homolog ikk1. This study focuses on a related protein kinase called Ripk4. The mouse homolog RIP4 is ...
I have been reading a lot about zebrafish research lately and I thought it would be nice to share some of my favorite articles with you.
The present approach of expressing hydrodynamic scaling in terms of regimes is useful for interpreting large-scale ontogenetic change but has the danger of concealing physically important phenomena. For example, it is informative that drag varies directly with speed and not to its second power in juvenile fish. However, it would be inaccurate to predict that larvae and juvenile fish should generate identical flow fields because they both operate in the viscous regime. Inertia is present and dynamically relevant at low Re, and viscosity is required for generating turbulence at high Re. Furthermore, drag coefficients may vary with Re within regimes, although to a lesser degree than observed across regimes (Fig. 5). Hydrodynamic regime categorization provides a useful first-order approximation of dominant hydrodynamic forces but is limited in its ability to predict the flow fields that generate those forces or higher-order effects. Nonetheless, our results provide an empirical basis for verifying ...
Description Liraglutide (LRT) ELISA Kit is an ELISA kit for the quantitative in vitro measurement of liraglutide (LRT) concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids. Introduction Liraglutide (NN2211) is a derivative of human incretin (metabolic hormone) glucagon-like peptide 1 (GLP-1) that is used as a long-acting glucagon-like […]. ...
In zebrafish[edit]. Zebrafish, like other teleost fish, possess the innate ability to regenerate retinal damage. This ability ... Retinal regeneration in zebrafish is mediated by Muller glia, which dedifferentiate into stem-like cells and proliferate into ... Rod precursors are produced during normal zebrafish growth and localize to the outer nuclear layer of the retina. In the event ... "β-catenin/Wnt signaling controls progenitor fate in the developing and regenerating zebrafish retina". Neural Development. 2012 ...
Ekker SC (2008). "Zinc finger-based knockout punches for zebrafish genes". Zebrafish. 5 (2): 121-3. doi:10.1089/zeb.2008.9988. ...
Ekker SC (2008). "Zinc finger-based knockout punches for zebrafish genes". Zebrafish. 5 (2): 121-3. doi:10.1089/zeb.2008.9988. ...
Genus information: Kallman, Klaus D.; Kazianis, Steven (2006). "The GenusXiphophorusin Mexico and Central America". Zebrafish. ...
Ekker SC (2008). "Zinc Finger-Based Knockout Punches for Zebrafish Genes". Zebrafish. 5 (2): 1121-1123. doi:10.1089/zeb. ... Similar research of using ZFNs to create specific mutations in zebrafish embryo has also been carried out by other research ... The kdr gene in zebra fish encodes for the vascular endothelial growth factor-2 receptor. Mutagenic lesions at this target site ... Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA (June 2008). "Targeted gene inactivation in zebrafish using engineered zinc ...
Zebrafish. 2 (4): 243-257. CiteSeerX doi:10.1089/zeb.2005.2.243. PMID 18248183. Koentges, G; Matsuoka, T (2002 ...
Kallman, K.D.; S. Kazianis (2006). "The genus Xiphophorus in Mexico and Central America". Zebrafish. 3 (3): 271-285. doi: ...
Grush J, Noakes DL, Moccia RD (February 2004). "The efficacy of clove oil as an anesthetic for the zebrafish, Danio rerio ( ... Hamilton)". Zebrafish. 1 (1): 46-53. doi:10.1089/154585404774101671. PMID 18248205. Monks, Neale (2 April 2009). "Aquarium Fish ...
S.C. Ekker (2008). "Zinc finger-based knockout punches for zebrafish genes". Zebrafish. 5 (2): 1121-3. doi:10.1089/zeb. ... zebrafish, various types of mammalian cells, and rats. An ongoing clinical trial is evaluating Zinc finger nucleases that ...
Page L, Polok B, Bustamante M, Schorderet DF (2013). "Bigh3 is upregulated in regenerating zebrafish fin". Zebrafish. 10 (3): ...
Price, Anna; Weadick, Cameron; Shim, Janet; Rodd, Frieda (2009-01-01). "Pigments, Patterns, and Fish Behavior". Zebrafish. 5 (4 ...
"KSI - Domain". Zebrafish. 25 December 2020. Retrieved 5 December 2020. "KSI - Domain". 25 December 2020. Retrieved 25 December ...
Her lab uses zebrafish as a genetic model to research retinal stem cells. From 1997 to 2002, Raymond was the associate provost ... Zebrafish. 2 (3): 157-164. 2005. doi:10.1089/zeb.2005.2.157. ISSN 1545-8547. "Raymond Lab , Molecular, Cellular, and ...
March 2013). "A sequence-based variation map of zebrafish". Zebrafish. 10 (1): 15-20. doi:10.1089/zeb.2012.0848. PMC 3629779. ... "Genome of Zebrafish Wild-type Strain (ASWT)". Retrieved 17 April 2013. "Detailed map of TB genome to help treatment". ... analysis of the first Malaysian Genome sequencing and analysis of the Wild-type strain of Zebrafish and the IndiGen programme ... colleagues at the Institute of Genomics and Integrative Biology announced the sequencing of the wild-type strain of zebrafish ...
Bill BR, Petzold AM, Clark KJ, Schimmenti LA, Ekker SC (March 2009). "A primer for morpholino use in zebrafish". Zebrafish. 6 ( ... Flynt AS, Li N, Thatcher EJ, Solnica-Krezel L, Patton JG (February 2007). "Zebrafish miR-214 modulates Hedgehog signaling to ... Draper BW, Morcos PA, Kimmel CB (July 2001). "Inhibition of zebrafish fgf8 pre-mRNA splicing with morpholino oligos: a ... These molecules have been applied to studies in several model organisms, including mice, zebrafish, frogs and sea urchins. ...
... "mc1r Pathway regulation of zebrafish melanosome dispersion". Zebrafish. 5 (4): 289-95. doi:10.1089/zeb.2008.0541. PMID 19133827 ... For example, zebrafish larvae are used to study how chromatophores organise and communicate to accurately generate the regular ... It is known in zebrafish embryos, for example, that by 3 days after fertilization each of the cell classes found in the adult ... It has been demonstrated that MC1R is required in zebrafish for dispersion of melanin. Inside the cell, cyclic adenosine ...
Clark KJ, Voytas DF, Ekker SC (September 2011). "A TALE of two nucleases: gene targeting for the masses?". Zebrafish. 8 (3): ...
Zebrafish given access to a barren, brightly lit chamber or an enriched chamber prefer the enriched area. When these fish are ... In contrast, zebrafish (Danio rerio) reduce their frequency of swimming and increase their ventilation rate but do not display ... Five-day old zebrafish larvae show a concentration dependent increase in locomotor activity in response to different ... When the same noxious stimulus is applied to zebrafish (Danio rerio), they respond by decreasing their activity. As with the ...
Fleisch, VC; Neuhauss, SCF (2006). "Visual Behavior in Zebrafish". Zebrafish. 3 (2): 191-201. doi:10.1089/zeb.2006.3.191. PMID ... In zebrafish, the optomotor response is frequently used as a metric of visual performance as it can be reliably evoked from 7 ... For instance, when zebrafish larvae are presented with a sinusoidal black and white grating pattern, the larvae will turn and ... Schmitt, EA; Dowling, JE (1994). "Early eye morphogenesis in the zebrafish, Brachydanio rerio". J Comp Neurol. 344 (4): 532-42 ...
Zebrafish and prairie voles have also become more common, especially in the social and behavioral scopes of neurogenetics. In ... Oliveira RF, Silva JF, Simões JM (June 2011). "Fighting zebrafish: characterization of aggressive behavior and winner-loser ... Burgess HA, Granato M (November 2008). "The neurogenetic frontier--lessons from misbehaving zebrafish". Briefings in Functional ... and zebrafish, to try to determine specific genes that correlate to behavior and attempt to match these with human genes. While ...
The Zebrafish Genome, which is being fully sequenced and manually annotated. The Zebrafish genome currently has 18,454 ... "Havana Project". Sprague, J. (1 January 2006). "The Zebrafish Information Network: the zebrafish model organism database". ... VEGA homepage Jen Harrow Bio WTSI homepage ENCODE homepage ZFIN Homepage Zebrafish Wild Type Strain Genome Zebrafish Genome ... It was designed to view manual annotations of human, mouse and zebrafish genomic sequences, and it is the central cache for ...
"Zebrafish SHHa". University of Oregon. Archived from the original on 2009-06-25. "Zebrafish SHHb". University of Oregon. ... In the zebrafish, two of the three vertebrate hh genes are duplicated: SHH a and SHH b (formerly described as tiggywinkle ... Currie PD, Ingham PW (August 1996). "Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish". Nature ... Lewis KE, Eisen JS (September 2001). "Hedgehog signaling is required for primary motoneuron induction in zebrafish". ...
JME - Pull Up". Zebrafish Media. 4 May 2019. Archived from the original on 11 July 2020. Retrieved 4 May 2019. "KSI & Randolph ...
Zebrafish Information Network. Retrieved 10 November 2017. CS1 maint: discouraged parameter (link) Westerfield, Monte; Doerry, ... and led the technology team that conceived of and built the Zebrafish Information Network, an international online multimedia ... Eckehard; Kirkpatrick, Arthur E.; Driever, Wolfgang; Douglas, Sarah Ann (1997). "An on-line database for zebrafish development ... database of information for zebrafish researchers. She is the author of over 70 technical papers and a research monograph, The ...
"Breeding Zebrafish" (Chapter 2). The Zebrafish Book. Retrieved 2006-05-02. CS1 maint: discouraged parameter (link) "Substrate ...
In zebrafish, Nkd genes regulate Wnt signaling. The mRNA transcription of Nkd genes is inducible by Wnt signaling in diverse ... Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning. Dev Biol. 2010 Dec 1;348(1):22-33. Epub 2010 ... Epub 2007 Apr 16.PMID 17438140 Van Raay TJ, Coffey RJ, Solnica-Krezel L. Zebrafish Naked1 and Naked2 antagonize both canonical ... Nkd3 in zebrafish). Mice with a mutant Nkd1 protein had a mild sperm maturation defect, but mice lacking both Nkd1 and Nkd2 ...
... is on the executive board of the European Zebrafish Society EZB e.V. and on the advisory board of the ... "Board". European Zebrafish Society. "Our Advisory Board". International Society for Regenerative Biology. "MRC Suffrage in ... The Becker group established the zebrafish as a model for spinal cord regeneration. Their research found that functional ... "Lesion-induced generation of interneuron cell types in specific dorsoventral domains in the spinal cord of adult zebrafish". ...
... , also known as zebrafish or stripey bream, is a species of marine ray-finned fish, a sea chub in the family ... Bray, D.J.; Gomon, M.F. (2011). "Zebrafish, Girella zebra". Taxonomic Toolkit for marine life of Port Phillip Bay. Museum ... Bray, D.J. (2018). "Girella zebra". Fishes of Australia. Museums Victoria. Retrieved 25 April 2020. Mark McGrouther (19 March ... Bray, D.; Gomon, M. (2018). "Girella zebra Zebrafish in Museums Victoria Collections". Museums Victoria. Retrieved 25 April ...
2006). "ZFIN Pharyngula Period Description". Zebrafish Information Network. Retrieved 2007-07-18. Haeckel E (1874) ...
Zebrafish: Danio rerio. Good embryo supply. Well developed genetics. Chicken: Gallus gallus. Early stages similar to mammal, ... "Forward and reverse genetic approaches for the analysis of vertebrate development in the zebrafish". Developmental Cell. 21 (1 ...
The Zebrafish Information Network (ZFIN) is an online biological database of information about the zebrafish (Danio rerio). The ... Vega Zebrafish Genome Annotation. *FishMap : The Zebrafish Community Genomics Browser maintained at the Institute of Genomics ... Sprague et al. (2008). The Zebrafish Information Network: the zebrafish model organism database provides expanded support for ... the China Zebrafish Resource Center (CZRC), and so on, which maintain and provide zebrafish-related research resources, ...
Enter the zebrafish larva. Small and transparent-yet able to swim freely and even hunt small prey-these baby fish have long ... Zebrafish Study Reveals First Fine Structure of a Complete Vertebrate Brain.. Despite decades of stunning advances in imaging ... Zebrafish larva head in cross-section. Note this isnt a single electron microscope image, but many thousands of slices seen on ... "What makes the zebrafish such a spectacular system is that the alternatives in other organisms for deriving wiring diagrams are ...
The first step of pronephrogenesis in the zebrafish is the formation of the intermediate mesoderm during gastrulation, which... ... Drummond IA (2005) Kidney development and disease in the zebrafish. J Am Soc Nephrol 16:299-304PubMedCrossRefGoogle Scholar ... Hu N, Yost HJ, Clark EB (2001) Cardiac morphology and blood pressure in the adult zebrafish. Anat Rec 264:1-12PubMedCrossRef ... Drummond IA, Davidson AJ (2010) Zebrafish kidney development. Methods Cell Biol 100:233-260PubMedCrossRefGoogle Scholar ...
A zebrafish can even regrow parts of its heart.. ...glow-in-the-dark. Through genetic modification you can make zebrafish glow ... Zebrafish. Zebrafish larvae have evolved into a very attractive model for drug discovery. Recently published methods facilitate ... Zebrafish embryos grow their organs the same way as humans, only 20 times faster and one zebrafish pair generates about 200 ... Roche is at the head of the class when it comes to the school of zebrafish research, and is pursuing the use of zebrafish in ...
Image 2 (below): Zebrafish with certain genes turned off during embryonic development (center and right images) showed ... As such, Sive and other researchers began looking at zebrafish genes that were analogous to the human genes found in region ... "This is really nice work that shows the importance of zebrafish in revealing disease mechanisms related to human mental ... the MIT study showed that almost all of them led to brain abnormalities when eliminated from zebrafish embryos. The results of ...
... zebrafish) tRNA (guanine(46)-N(7))-methyltransferase (zebrafish) methyltransferase-like protein 1 (zebrafish) ... A tRNA (guanine-N(7)-)-methyltransferase that is encoded in the genome of zebrafish. [ PRO. :. DNx OMA. :. Q5XJ57 ] ... Synonyms: z-METTL1 tRNA(m7G46)-methyltransferase (zebrafish) miRNA (guanine-N(7)-)-methyltransferase ( ... tRNA (guanine-N(7)-)-methyltransferase (zebrafish). Go to external page Copy ...
Zebrafish and Skin Color. This is a part of The Biology of Skin Color ... This lesson complements the film The Biology of Skin Color and explores how experimental work in zebrafish led to a better ...
Rodents and zebrafish against cancer. With the help of animal models, scientists have extended the lives of pancreatic cancer ... In another study led by Zon, the team examined effects of various chemical compounds on pigments in zebrafish (and frogs too). ... Zebrafish can develop a form of the disease genetically similar to human skin cancer tumors. ... Genetically engineered mice and zebrafish enabled new research towards treatments for pancreatic and skin cancers, respectively ...
Genetically engineered zebrafish could provide a quick way to test new drugs to prevent dangerous plaque buildup in the heart. ...
Zebrafish publications at Brown University (in PubMed). JoVE video on the automated analysis of behavior in zebrafish larvae ( ...
Scientists became interested in zebrafish only in the 1960s, today the little fish are one of the most popular organisms in ... Zebrafish are unassuming and undemanding. Almost every child knows that - at least any child that has kept fish in an aquarium ... Scientists only discovered the zebrafish, which is a member of the minnow family, for their own purposes in the late 1960s. In ... As a vertebrate, the zebrafish has many genes that have the same, or similiar, functions in mammals and thus also in humans. ...
The zebrafish, Danio rerio, has emerged as a powerful model system for the study of vertebrate development and disease. This ... Lectures and labs will introduce students to early development of the zebrafish and to methods for manipulating and studying ... It will cover standard and novel technologies geared towards their application in zebrafish. Mornings and afternoons will be ... Please see the 2019 MBL zebrafish course poster for more information and to apply. ...
A sequence-based variation map of zebrafish.. Zebrafish. 2013 Mar;10(1):15-20 [Highlighted on the cover of the journal]. For ... 17th July 2009 , Zebrafish Wild Type Strain Genome was presented at the 6th European Zebrafish Genetics and Development Meeting ... Talk Title: WHOLE-GENOME SEQUENCING OF A WILDTYPE STRAIN OF ZEBRAFISH FOR VARIANT DISCOVERY. View abstract of the talk. 27th ... Wild Zebrafish Genome Scientific Article. Patowary A, Purkanti R, Singh M, Chauhan R,Singh AR, Swarnkar M, Singh N, Pandey V, ...
Zebrafish Resources can be found at the central website of the zebrafish community at ZFIN. For example, here you can search ... The Zebrafish Community meets biannually at the International Conference on Zebrafish Development and Genetics, which usually ... recipes and protocols in the online version of the famous Zebrafish Book or the ZFIN Protocol Wiki or for ... The biannual Asia Oceania Zebrafish Meeting alternates with the International Conference and takes place at changing locations ...
Zebrafish develops cancers spontaneously, after chemical mutagenesis or through genetic manipulations. Furthermore, zebrafish ... Finally, zebrafish embryos are used in molecule screens and contribute to the identification of novel anti-cancer therapeutic ... Although cell culture and mouse models will remain a cornerstone of cancer research, the unique capabilities of the zebrafish ... Xenotransplantation of human cancer cells in embryos or adult zebrafish presents the advantage of following cancer cell ...
Toxicological and pharmacological researchers have seized upon the many benefits of zebrafish, including the short generation ... The abundance of sites with non-reference alleles per T5D zebrafish could imply that within a population, zebrafish are more ... 2016), an individual zebrafish within the T5D population may vary more from the current zebrafish reference genome than ... zebrafish). While more variants have been discovered in the human and mouse genomes, the smaller zebrafish genome is on par ...
Epiboly plays a prominent role in zebrafish gastrulation and studies of zebrafish epiboly have provided insights into basic ... Zebrafish epiboly: mechanics and mechanisms.. Lepage SE1, Bruce AE.. Author information. 1. Department of Cell and Systems ... Here, we review work on the molecular and cellular basis of zebrafish epiboly in order to identify unifying themes and to ...
... techniques for developing and analyzing zebrafish disease models, and methods for neuroscience. Zebrafish: Methods and ... This second edition details new emerging areas of zebrafish research focusing on genetics and genomics, ... Zebrafish: Methods and Protocols, Second Edition guides readers through methods for mutagenesis and genome editing in zebrafish ... Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a useful complement to the first book for new ...
Figure 1: Zebrafish thrombocyte electron micrographs. (a) Zebrafish thrombocyte. Open canalicular like system is shown by ... Development of Zebrafish Model for Thrombopoiesis. In zebrafish, hematopoiesis has been extensively studied [45, 46]. There are ... S. C. Ekker, "Zinc finger-based knockout punches for zebrafish genes," Zebrafish, vol. 5, no. 2, pp. 121-123, 2008. View at ... 2. Development of Zebrafish Model to Study Thrombocyte Function. Unlike mammalian platelets which are anucleated, zebrafish ...
Zebrafish floating head (flh) mutants lack a notochord, but develop with prechordal plate and other mesodermal derivatives, ... We show that floating head is the zebrafish homologue of Xnot, a homeobox gene expressed in the amphibian organizer and ... Zebrafish floating head (flh) mutants lack a notochord, but develop with prechordal plate and other mesodermal derivatives, ... Talbot, W., Trevarrow, B., Halpern, M. et al. A homeobox gene essential for zebrafish notochord development. Nature 378, 150- ...
The zebra fish is widely used in molecular and developmental genetic engineering studies. Our Zebra Fish are hand-selected for size and health, so you can enjoy them for years to come.
... 15.06.2010. This image may bring to mind a patchwork quilt, or a picture taken from a gallery ... As a zebrafish embryo develops, the cells that will form its lateral line have to become pointed at one end and arrange ... Darren Gilmours group at EMBL captured this snapshot of the beginnings of an organ which plays a central role in how zebrafish ...
Disclosure: Walyou is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to ...
... Riki Dayan via (by rdayan from Tue Mar 16 17:29:10 EST 2010 * ... Does anyone have any suggestions on where to find these? Ive checked with the Zebrafish International Resource Center but the ... Hi, I am studying visual opsins and pigmentation aggregation/dispersion and am looking for an albino zebrafish. ...
The Zebrafish International Resource Center (ZIRC) The European Zebrafish Resource Center (EZRC) The China Zebrafish Resource ... Zebrafish have been used as a model system in environmental toxicology studies. Zebrafish have been used as a model system to ... In research, adult zebrafish are often fed with brine shrimp, or paramecia. Zebrafish are hardy fish and considered good for ... Zebrafish is widely used as model organism to study muscular dystrophies. For example, the sapje (sap) mutant is the zebrafish ...
A study of zebrafish has revealed ways to potentially slow or prevent the onset of cancerous tumors, the Huntsman Cancer ... Using zebrafish that share genetics similar to humans, researchers discovered two enzymes that, when combined, could be ... 24 (UPI) -- A study of zebrafish has revealed ways to potentially slow or prevent the onset of cancerous tumors, the Huntsman ...
The zebrafish, a translucent fish often used as a model of human development and disease, offers unique advantages for studying ... A particular advantage of using zebrafish to study cancer biology is the ability to transplant human tumors into the fish using ... Their paper "p53 Isoform à "113p53 in Zebrafish" discusses the potential use of this particular p53 isoform for characterizing ... In the paper entitled "Identification of a Heritable Model of Testicular Germ Cell Tumor in the Zebrafish," authors Joanie ...
Purchase Essential Zebrafish Methods: Genetics and Genomics - 1st Edition. Print Book & E-Book. ISBN 9780123751607, ... Cloning Zebrafish by Nuclear Transfer (Shuo Lin). 15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In ... 3. The Zebrafish Genome (John Postlethwait) 4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish (Adam Amsterdam) 5. ... Transgene Manipulation in Zebrafish by Using Recombinases (Gary W. Stuart). 13. Highly Efficient Zebrafish Transgenesis ...
Tropical zebrafish have helped researchers at the University of Sheffield pave the way for the discovery of new anti- ... They were able to show that a number of these drugs worked in zebrafish, thereby establishing that zebrafish could be used to ... "Zebrafish Play Pivotal Role In Helping Treat Disease." Medical News Today. MediLexicon, Intl., 30 Oct. 2009. Web.. 20 Aug. 2018 ... Tropical zebrafish have helped researchers at the University of Sheffield pave the way for the discovery of new anti- ...
... are shown inside the gut of a larval zebrafish. The gut is visible as a gray background. The image is a compilation of a 3.5- ... The rapid trajectories of Vibrio cholerae bacteria, shown in blue, are shown inside the gut of a larval zebrafish. The gut is ... In zebrafish, the cholera bacterium sets off a surprising flush. University of Oregon ...
  • The Zebrafish Information Network ( ZFIN ) is an online biological database of information about the zebrafish ( Danio rerio ). (
  • The zebrafish, Danio rerio , has emerged as a powerful model system for the study of vertebrate development and disease. (
  • Institute of Genomics and Integrative Biology ( ), which is a constituent laboratory of the Council of Scientific and Industrial Research ( ) has completed the whole genome sequencing of a Wildtype Strain of Zebrafish (Danio rerio). (
  • The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family (Cyprinidae) of the order Cypriniformes. (
  • The zebrafish has often been referred to as "Danio rerio", but more recent molecular studies have suggested that it should belong to the genus Brachydanio, as Brachydanio rerio. (
  • In this regard, we wondered whether Danio rerio (Zebrafish) previously used as a genetic model to study developmental biology could be used as a genetic model to study hemostasis especially platelet biology [ 1 ]. (
  • A male (7-8 months old) and a female (15-16 months old) zebrafish (Danio rerio), supplied from an importing company and brought to biotechnology laboratory of Istanbul University Faculty of Fisheries Department of Aquaculture, have been used in the study. (
  • Scientifically known as Danio rerio, zebrafish get their name from the horizontal blue stripes that run along the sides of their body. (
  • Tiny fish, big research potential: zebrafish (Danio rerio) share 75 per cent of their genome with humans. (
  • Although zebrafish ( Danio rerio ) behavior has been used to rapidly identify neuroactive compounds, it is not clear what types of behavioral assays would be necessary to identify multitarget compounds such as antipsychotics. (
  • Danio rerio commonly known as zebrafish is a tropical fish belonging to the minnow family (Cyprinidae). (
  • The zebrafish ( Danio rerio ) is a powerful vertebrate model system for studying development, modeling disease, and screening for novel therapeutics. (
  • Over 60 years after the initial description of PDE, we report the first animal model for this disease: an aldh7a1-null zebrafish ( Danio rerio ) displaying deficient lysine metabolism and spontaneous and recurrent seizures in the larval stage (10 days postfertilization). (
  • The zebrafish ( Danio rerio ) is a popular research model. (
  • Genome-wide chemical mutagenesis screens in the zebrafish (Danio rerio) have led to the identification of novel genes affecting vertebrate erythropoiesis. (
  • 2 3 Many of the genes known to regulate erythropoiesis in mammals have been identified in another vertebrate animal model, the zebrafish (Danio rerio) . (
  • Agillent׷ Zebrafish Promoter ChIP-on-chip Microarray 2-slide set enables identification of Danio rerio DNA binding proteins by combining chromatin IP pull-down assays with DNA microarray location analysis. (
  • Long-term repeatability of behaviours in zebrafish ( Danio rerio ). (
  • Mitochondrial genome diversity among six laboratory zebrafish ( Danio rerio ) strains. (
  • Zebrafish embryos grow their organs the same way as humans, only 20 times faster and one zebrafish pair generates about 200 eggs per week. (
  • While most of the genes´ functions were not known, the MIT study showed that almost all of them led to brain abnormalities when eliminated from zebrafish embryos. (
  • Because of the optical transparency of the embryos, small size at maturity and ease of culture, Zebrafish has become a popular organism to study embryonic development for biologist worldwide. (
  • Xenotransplantation of human cancer cells in embryos or adult zebrafish presents the advantage of following cancer cell behavior in vivo. (
  • Finally, zebrafish embryos are used in molecule screens and contribute to the identification of novel anti-cancer therapeutic strategies. (
  • Toxicological and pharmacological researchers have seized upon the many benefits of zebrafish, including the short generation time, well-characterized development, and early maturation as clear embryos. (
  • Using the Tanguay lab Tropical 5D zebrafish line (T5D), we performed whole genome sequencing on a large group ( n = 276) of individual zebrafish embryos. (
  • 2013 ). There are many benefits to using zebrafish in developmental studies, including early maturation as clear embryos that are amenable to easily observable morphological endpoints, short generation time, and well-characterized development that is conserved across species during the phylotypic period (Kimmel et al. (
  • According to FDA research biologist Jyotshna Kanungo, Ph.D., "Using the embryos of zebrafish as models, we study how various drugs and chemicals affect the organs and systems. (
  • Using transgenic (genetically-altered) zebrafish embryos, scientists are investigating the effect of copper nanoparticles-frequently used in medical devices, paints and fabrics-on biological systems. (
  • Deneke's current work involves using Zebrafish, which are used because the eggs are fertilized and develop outside the mother's body and the embryos are nearly transparent. (
  • 2 Zebrafish embryos develop much more rapidly than mammals, and thus using zebrafish embryos as a model organism is efficient for toxicological and other studies. (
  • Unlike many other model organisms, one of the major advantages of zebrafish is that the embryos develop externally and are optically transparent. (
  • Also, adult zebrafish can produce hundreds of embryos per day. (
  • 3 General toxicology screens can capture a broad range of phenotypes in wild-type (WT) zebrafish embryos. (
  • Zebrafish embryos can be housed in individual microplate wells for live-animal assays. (
  • We set up an experimental system to study the zIFN system in the course of a viral infection of zebrafish embryos. (
  • The zebrafish homologue of the human myeloperoxidase (MPO) gene, which is specific to cells of the neutrophil lineage, was cloned and used to synthesize antisense RNA probes for in situ hybridization analyses of zebrafish embryos. (
  • By exposing zebrafish embryos to the thyroid hormone triiodothyronine (T3) we showed that exposure accelerates ossification of craniofacial elements including the opercle and ceratohyal in a dose-dependent manner. (
  • Identifying novel therapeutics for DBA and 5q- MDS using high content screening of zebrafish embryos as shown in Figure 2. (
  • of human coding genes have an equivalent in zebrafish. (
  • Very recently, a number of methods have been optimised that simplify the genetic engineering of these little fish, and allow them to express a variety of human and/or disease relevant genes in zebrafish larvae. (
  • Contrary to the fruit fly, Drosophila, and the worm, C. Elegans, which have been pivotal in our understanding of genes and phenotypes, zebrafish are vertebrates, and therefore much more relevant for human physiology in health and disease. (
  • In particular, Massachusetts Institute of Technology ( MIT ) researchers recently revealed that they took a fresh approach to understanding the roles of genes linked to autism and demonstrated how zebrafish could be utilized in examining the genes that contribute to human brain disorders. (
  • As such, Sive and other researchers began looking at zebrafish genes that were analogous to the human genes found in region 16p11.2. (
  • Image 2 (below): Zebrafish with certain genes turned off during embryonic development (center and right images) showed abnormalities of brain formation (top row) and axon wiring (bottom row). (
  • In the early 1980s, researchers managed to breed zebrafish with identical genes, known as homozygotes. (
  • As a vertebrate, the zebrafish has many genes that have the same, or similiar, functions in mammals and thus also in humans. (
  • Over the years, studies in zebrafish have enabled discovery of novel genes, pathways and models of human diseases in addition to understanding mechanisms underlying genome evolution. (
  • Since we introduced the zebrafish model for the study of hemostasis and thrombosis, other groups have adapted this model to study genes that are associated with thrombocyte function and a few novel genes have also been identified. (
  • Noting the "relative ease and low costs of transgenesis"""putting human genes into zebrafish""and the unique benefits of working with zebrafish, especially related to imaging, genetics, and transplantation, Dr. Leach predicts that, "Future zebrafish cancer research exploiting these fundamental advantages will be especially likely to generate novel insights not achievable using other model systems," in his Introduction to the issue entitled, "Pisces and Cancer: The Stars Align. (
  • The predicted GST genes of common carp were named based on their zebrafish orthologs, corrected by phylogenetic topologies. (
  • Sequencing revealed that 71.4% of human genes have at least one zebrafish ortholog. (
  • Currently the zebrafish genome is thought to have more than 26,000 protein-coding genes. (
  • Functional investigation of a subset of these genes, fgf10a, tgfb2, pax9, and smad5 revealed their necessity in zebrafish palatogenesis. (
  • Albert Pan (left), an associate professor at the Fralin Biomedical Research Institute, and Manxiu 'Michelle' Ma, a research associate in the Pan lab, were part of a team of scientists who worked with zebrafish to discover that genes linked to autism spectrum disorder and other brain abnormalities may be playing a role in people who cannot control their eye movements. (
  • Researchers studying zebrafish have found that genes linked to autism spectrum disorder and other developmental brain abnormalities may be playing a role in people who cannot control their eye movements. (
  • Harvesting lab-raised zebrafish based on their size led to differences in the activity of more than 4,000 genes, as well as changes in allele frequencies of those genes, in the fish that remained. (
  • Participants chronicled their use of zebrafish to assess drug toxicity and efficacy, to find bioactive small molecules, and even to find genes coding for potential new drug targets. (
  • Postdoctoral fellow Zurab Tsetskhladze, who performed the zebrafish experiments, tested this method with two genes that affect skin color. (
  • The expression of zebrafish homologues was also examined in 2 other mammalian hematopoietic genes, Pu.1 , which appears to initiate a commitment step in normal mammalian myeloid development, and L-Plastin , a gene expressed by human monocytes and macrophages. (
  • The results demonstrate a high level of conservation of the spatio-temporal expression patterns of these genes between zebrafish and mammals. (
  • 13 This conservation of the fundamental mechanisms controlling erythropoiesis between mammals and zebrafish 4 raises the possibility that this powerful genetic system could be applied to uncover new classes of genes required in the normal development of granulocyte/macrophage cell lineages during vertebrate myelopoiesis. (
  • Scientists from the Duke University analyzed all of the zebrafish genes whose activity suddenly changed immediately following a spinal injury. (
  • We use zebrafish with knockdown of the ribosomal protein genes Rps19, Rps26 and Rpl11 in during development to model the congenital red cell aplasia, Diamond-Blackfan Anaemia (DBA). (
  • With the help of co-author PSC's Art Wetzel, they led an international collaboration that used these images to reconstruct specific nerve cells that spanned nearly the entire larval zebrafish brain. (
  • Our goal [was] to develop techniques that allow researchers to examine the morphology and circuit connectivity of any neuron in the brain of a larval zebrafish at about five days after fertilization. (
  • The research team led by Dr Stephen Renshaw, Senior Clinical Lecturer in the Clinical Academic Unit of Respiratory Medicine at the University, has set-up a system for drug screening after finding important parallels between inflammation in the 3mm long larval fish (a similar stage to a tadpole) and in humans - proof that the zebrafish can be used to find anti-inflammatory drugs. (
  • The rapid trajectories of Vibrio cholerae bacteria, shown in blue, are shown inside the gut of a larval zebrafish. (
  • Here we present an automated high-throughput system for measuring nonassociative learning behaviors in larval zebrafish. (
  • Using this system, we report that spaced training blocks of repetitive visual stimuli elicit protein synthesis-dependent long-term habituation in larval zebrafish, lasting up to 24 h. (
  • Although we found extensive functional conservation of short-term learning between larval zebrafish and mammalian models, we also discovered several compounds with previously unknown roles in learning. (
  • Larval zebrafish execute a repertoire of simple, well-defined, and stereotyped sensorimotor behaviors that have accessible and characterized circuitry and provide a vertebrate system amenable to large-scale forward genetic and chemical screening ( 17 ⇓ ⇓ ⇓ ⇓ ⇓ ⇓ - 24 ). (
  • Here we developed a battery of behavioral assays in larval zebrafish to determine whether behavioral profiles can provide sufficient phenotypic resolution to identify and classify psychiatric drugs. (
  • Zebrafish have the ability to regenerate many of their tissues including fins, skin, heart and, in larval stages, brain. (
  • The facility is equipped with 48 racks which hold standard sized adult zebrafish tanks, a three rack nursery space for larval fish, and a quarantine space containing two stand-alone housing systems. (
  • Researchers unveil the neural basis of alertness in larval zebrafish. (
  • According to the authors, this was the first time abnormal behavior of larval zebrafish to cadmium and aluminum has been compared. (
  • The zebrafish is a widely used model organism for genetic , genomic , and developmental studies, and ZFIN provides an integrated interface for querying and displaying the large volume of data generated by this research. (
  • Through genetic modification you can make zebrafish glow in the dark in different colours. (
  • Genetic screens in Zebrafish have led to the discovery of large number of mutations that disrupt embryonic development. (
  • Zebrafish develops cancers spontaneously, after chemical mutagenesis or through genetic manipulations. (
  • These data were used to compare observed population genetic variation across species (humans, mice, zebrafish), then across lines within zebrafish. (
  • We establish T5D as a model that is representative of diversity levels within laboratory zebrafish lines and demonstrate that experimental design and analysis can exert major effects when characterizing genetic diversity in heterogeneous populations. (
  • Keeping this in mind we introduced zebrafish, a vertebrate genetic model to study platelet function. (
  • In the paper entitled "Identification of a Heritable Model of Testicular Germ Cell Tumor in the Zebrafish," authors Joanie Neumann, Jennifer Dhepard Dovey, Garvin Chandler, Liliana Carbajal, and James Amatruda, describe the development of a zebrafish model that carries a genetic mutation making the fish highly susceptible to the development of testicular tumors. (
  • Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. (
  • The zebrafish is currently being utilised to study novel genetic factors hypothesised to contribute to osteoporosis. (
  • This second volume covers advances in forward and reverse genetic techniques, provides an update on the zebrafish genome and gene/mutant mapping technologies, examines the new systems for efficient transgenesis in the zebrafish, provides an in-depth view of informatics and the emerging field of comparative genomics, and considers the extensive infrastructure now available to the zebrafish community. (
  • Recent studies have shown that the zebrafish palate, or anterior neurocranium, is under similar genetic control to the amniote palatal skeleton. (
  • To compare the relative recombination rates during meiosis in male and female zebrafish, we constructed a genetic map based on male meiosis. (
  • We used a forward genetic screen in zebrafish to identify the transcription factor mafba as essential for lymphatic vessel development. (
  • Owing to a high quality genome reference sequence and its genetic and embryological tractability the zebrafish is a vertebrate model especially suited for large scale studies. (
  • The zebrafish is a potential tool for testing one class of unique individual genetic differences found in humans, and may yield information helpful for the emerging field of personalized medicine, according to a team led by Penn State College of Medicine scientists. (
  • Zebrafish can be used as a model to understand what biological effects result from these genetic mutations. (
  • The zebrafish is a good choice because of its similarity to humans as a vertebrate, its transparency as an embryo and the powerful genetic tools available in this model organism. (
  • Cheng's lab works with zebrafish to study genetic differences that contribute to human skin color. (
  • The morphologic and molecular genetic evidence presented here supports the zebrafish as an informative model system for the study of normal and aberrant human myelopoiesis. (
  • We (humans) share 70 per cent of our genetic code with zebrafish, and the human CTGF protein is nearly 90% similar to the one from the zebrafish. (
  • In parallel we have developed a zebrafish with loss of Rps14 which is thought to be the major genetic determinant in the anaemia observed in patients with a subtype of myelodysplastic syndrome, the 5q minus syndrome. (
  • ZFIN's community wiki gives zebrafish researchers the ability to share information about laboratory protocols and antibodies. (
  • To this end, researchers at Harvard and PSC used PSC-developed software and hardware to create a 3D map of the zebrafish larva brain-the first whole vertebrate brain so reconstructed. (
  • laboratories and researchers working on zebrafish. (
  • Using zebrafish that share genetics similar to humans, researchers discovered two enzymes that, when combined, could be involved in the earliest stages of cancer, the Salt Lake City-based Institute announced Wednesday. (
  • Tropical zebrafish have helped researchers at the University of Sheffield pave the way for the discovery of new anti-inflammatory drugs to treat illnesses such as chronic obstructive pulmonary disease (COPD) and asthma . (
  • In a series of studies, researchers tested how zebrafish behaved after receiving doses of Lactobacillus plantarum, a common bacteria found in yogurt and probiotic supplements. (
  • Researchers develop a new method to highlight specific cells that reside in the lens of a zebrafish. (
  • To explore whether this gene might play a role in preventing tissue regeneration in humans, the researchers added human ARF to the zebrafish genome and assessed how it affected the fishes' normal ability to regrow damaged fins after injury. (
  • As one of 22 early-career researchers selected as Pew Biomedical Scholars in 2011 , Morris opted to watch eye development-and its potential mishaps-in action within zebrafish. (
  • Researchers from the University of Pittsburgh are using zebrafish to study the biological mechanisms that lead to intestinal inflammation, as often seen in IBD, providing additional understanding that may allow development of better therapies. (
  • Well, not exactly, but for the first time researchers have followed the growth of each cell in a zebrafish embryo as it grew from one to 20,000 cells strong. (
  • The researchers found that the change made no difference in function in zebrafish. (
  • ZFIN is home to the Zebrafish Model Organism Database-A directory for researchers to access zebrafish-related resources. (
  • Researchers hope that better understanding the repair mechanisms in zebrafish could eventually lead to new therapies for people with neurodegenerative conditions. (
  • At Oregon, Douglas was a member of the Cognitive and Decision Sciences Institute(Director, 1995-1998) and led the technology team that conceived of and built the Zebrafish Information Network, an international online multimedia database of information for zebrafish researchers. (
  • We characterized zebrafish thrombocytes and established functional assays study not only their hemostatic function but to also their production. (
  • Authors Leonard Zon, PhD, and Alison Taylor, PhD, from Harvard Medical School and Children's Hospital Boston present the concepts and techniques relevant to zebrafish transplantation assays. (
  • They describe how tumor transplantation has been used to study leukemia, rhabdomyosarcoma, and melanoma in the paper "Zebrafish Tumor Assays: The State of Transplantation. (
  • Despite the abundance of established habituation learning assays for adult rodent and zebrafish models, scaling these assays for systematic approaches is challenging given the inherent complexity and variability of adult behaviors, as well as the time required to train and test large numbers of animals ( 10 ⇓ ⇓ ⇓ ⇓ ⇓ - 16 ). (
  • Although some studies have explored the capacity of zebrafish larvae for various forms of short-term learning and sensory conditioning, these assays have not been adapted to systematic approaches ( 25 ⇓ - 27 ). (
  • Zebrafish combine the convenience and throughput of assays carried out in a multiwell plate with the benefits of performing assays in a fully functioning organism. (
  • To produce in vivo phenotypes in zebrafish assays, compounds must exhibit the ability to be absorbed, reach the target tissue, and avoid rapid metabolism and excretion. (
  • Chemists' burgeoning interest in using zebrafish to complement other animal models and cell-based assays motivated an afternoon session sponsored by the Division of Chemical Toxicology at the American Chemical Society national meeting in Boston last month, according to organizer Peter C. Dedon , a professor of toxicology and biological engineering at Massachusetts Institute of Technology. (
  • Several informal 'roundtable' discussions will cover the design and execution of mutant screens/reverse genetics, transgenesis, disease modeling, zebrafish breeding and husbandry, as well as data management and figure construction. (
  • A major difference from many model organisms is that standard husbandry practices in zebrafish are designed to maintain population diversity. (
  • Since 2011, a full time manager staffs the facility and is available for assistance with access, training and general Zebrafish husbandry questions. (
  • The student will receive a thorough education in molecular biology, zebrafish husbandry, fluorescence microscopy and handling of large datasets. (
  • Prior knowledge and experience in fluorescence microscopy, bioinformatics, programming, zebrafish husbandry and/or image-based analyses are a bonus. (
  • This video will provide an overview of zebrafish husbandry and maintenance in the lab. (
  • The organ make up and general body plan of a zebrafish is similar to humans because a zebrafish is a vertebrate. (
  • While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development. (
  • They were able to show that a number of these drugs worked in zebrafish, thereby establishing that zebrafish could be used to identify new potential therapeutics for humans. (
  • Significantly, the zebrafish ECM were also found to offer stress-protection to specialized cells that make up the heart muscle in humans, called cardiac myocytes. (
  • Surrounded by 288 fish tanks in her lab at NCTR, Kanungo notes that unlike the fruit flies and the nematode worm, other popular species studied in laboratories, zebrafish are vertebrates (have a backbone), as are humans. (
  • For example, the hearts of zebrafish and humans both have chambers and rhythmically pump oxygen-carrying blood through the body. (
  • Zebrafish have neuromasts (small sensory patches) similar to those lining the inner ear of humans. (
  • The necessary molecular transitions required for development take place in similar sequences but occur much more rapidly in zebrafish compared to humans. (
  • Zebrafish are close enough to humans on a molecular level to be relevant, and their development is fast enough to make it a convenient model to employ for investigation. (
  • Insights from creatures like zebrafish and salamanders, which routinely regrow damaged tails, limbs, jaws and even hearts, may one day endow humans with heightened regenerative abilities. (
  • Now, as a plastic surgeon and stem cell researcher, he believes that insights from creatures like zebrafish and salamanders, which routinely regrow damaged tails, limbs, jaws and even hearts, may one day endow humans with heightened regenerative abilities. (
  • And unlike humans, adult zebrafish can regenerate damaged tissues and organs, including eyes. (
  • In the current study, two of the amino acid differences that Cheng has shown in prior studies to contribute to light skin color in humans prevented the zebrafish color from darkening. (
  • Zebrafish are increasingly used as a model for neurotoxic effects because the neuropathological behavior is relatable to that shown in humans. (
  • At the molecular level, the biological similarity between zebrafish and humans is striking (Spoorendonk et al. (
  • Genetically engineered mice and zebrafish enabled new research towards treatments for pancreatic and skin cancers, respectively. (
  • We evaluated whether alterations in the balance between ERK1/2 and PI3K signaling pathways could stimulate arterial formation in the setting of defective arterial morphogenesis in mice and zebrafish. (
  • While this work has only just begun, it may eventually shed new light on past studies of zebrafish behavior-and point the way toward a better understanding of more complex brains, such as ours. (
  • Epiboly plays a prominent role in zebrafish gastrulation and studies of zebrafish epiboly have provided insights into basic cellular properties and mechanisms of morphogenesis that are widely used in animal development. (
  • Robotic automation specialists Hudson Robotics, along with Johns Hopkins University, won an NIH grant to develop a commercially viable high-throughput screening (HTS) system for in vivo studies of zebrafish. (
  • The focus of the present paper is on spinal cord regeneration in adult zebrafish. (
  • Unlike mammals, zebrafish retains radial glia, a nonneuronal cell type in their adult central nervous system. (
  • In addition to being small, adult zebrafish are easy to maintain compared to mammalian models, such as mice and rats. (
  • A three-dimensional reconstruction of the entire depth of the front part of the brain (forebrain) of an intact living adult zebrafish imaged with three-photon microscopy (3PEF). (
  • In previous studies, the team in Naples showed some neurotoxic effects of cadmium and aluminum in adult zebrafish during embryological development. (
  • Zebrafish, a four centimeter long fish native to Indian rivers, has attracted considerable scientific interest worldwide primarily as a non-mammalian vertebrate model organism. (
  • Because repeated observations for the replicate chemicals in the Phase I data set were not available for mammalian or zebrafish data, comparisons between species were analyzed using averaged C. (
  • Several studies have found strikingly similar mammalian and zebrafish toxicology profiles. (
  • Here we present the identification and characterization of a zebrafish APC subset strongly resembling mammalian DCs. (
  • 4 Homologues of known mammalian hematopoietic transcription factors, such as gata-1, gata-2, c-myb , scl, fli-1, Imo2 , and cbfb are all appropriately expressed during zebrafish embryogenesis. (
  • The study, which was conducted in the University's MRC Centre for Developmental and Biomedical Genetics, is the first published proof of principle work to show that zebrafish models can be used to find anti-inflammatory drugs. (
  • Consequently, the zebrafish is an efficient and convenient model for many developmental and toxicological studies. (
  • Developmental hemapoiesis in zebrafish and Antarctic fishes. (
  • Zebrafish represent an important vertebrate model species in developmental biology. (
  • Zebrafish larvae display a high amount of developmental plasticity, enabling an adaptive response to training. (
  • In determining if this approach could also be used to clarify the molecular genetics of myelopoiesis, it was found that the developmental hierarchy of myeloid precursors in the zebrafish kidney is similar to that in human bone marrow. (
  • Furthermore, transgenic zebrafish with GFP-tagged thrombocytes have been developed which helped to study the production of thrombocytes and their precursors as well as their functional roles not only in hemostasis but also hematopoiesis. (
  • Jun Chen and Jinrong Peng, from Zhejian University (China), describe the use of transgenic zebrafish to understand the roles that different naturally occurring forms of the tumor suppressor gene p53 play in regulating cell cycle, metabolism, organ development, and cell aging and death. (
  • By watching human breast cancer cells in real time in the live transgenic zebrafish, we were able to determine that two proteins are required in order for breast tumor cells to complete the most critical step of metastasis - entering the blood vessels," said Konstantin Stoletov, Ph.D., of the department of pathology at the UC-San Diego School of Medicine, first author of the paper. (
  • Scientists only discovered the zebrafish, which is a member of the minnow family, for their own purposes in the late 1960s. (
  • Why do scientists study zebrafish? (
  • This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world's leading scientists. (
  • Zebrafish are useful to scientists because they have a fully functioning simple heart and circulatory system. (
  • Scientists at the University of California, San Diego (UCSD) School of Medicine are working with transparent zebrafish to study cancer cells in action. (
  • The UCSD team developed an immuno-suppressed zebrafish that expresses green fluorescent protein (commonly known as GFP) only in its blood vessels, allowing scientists to view the tumor-induced blood vessel formation, or angiogenesis. (
  • Mutant zebrafish called "Casper" are transparent, allowing scientists to easily monitor cancer growth. (
  • Also, since scientists have used zebrafish to model disease for decades, their genetics are well-understood and easily manipulated. (
  • The largest survey to-date on the use and care of zebrafish in research, conducted by scientists at the NC3Rs, AstraZeneca and University of Plymouth. (
  • Scientists have discovered that a hormone called serotonin - better known for its role as a mood booster - can help zebrafish to recover from a spinal cord injury. (
  • Even more interestingly, when scientists injected human CTGF in to a zebrafish, they observed that the spinal cord could still be regenerated. (
  • In fact, about 71% of human proteins have an ortholog in zebrafish. (
  • The aim of this thesis was to analyze the composition of the extracellular matrix in zebrafish, in order to enable the identification of potential key regulatory proteins. (
  • After the identification of potential regulatory proteins in the developing zebrafish skeleton during the MS-based approach, the protein carboxypeptidase Z (Cpz) was selected for further analysis. (
  • Like the human diseases arising from loss of ribosomal proteins, zebrafish with loss of these ribosomal proteins show evidence of a profound anaemia. (
  • This is achieved through our partners, the Zebrafish International Resource Center (ZIRC) and the European Zebrafish Resource Center (EZRC), who provide distribution services for the zebrafish community. (
  • Collectively, these results suggest that the gene regulatory networks regulating palatogenesis may be conserved across vertebrate species, demonstrating the utility of zebrafish as a model for palatogenesis. (
  • This figure is a composition of two intercalated confocal micrographs showing the detail view of the habenular nucleus of a wild-type and a mutant zebrafish embryo. (
  • Prakash Thakur, a research associate working with Nathan Bahary, M.D., Ph.D., described a mutant zebrafish strain that shows many pathological characteristics similar to IBD, including inflammation, abnormal villous architecture, disorganized epithelial cells, increased bacterial growth and high numbers of dying cells in the intestine. (
  • He started with an equivalent of the broken car part: mutant zebrafish with lighter pigment cells. (
  • First, Tsetskhladze confirmed that by injecting normal messenger RNA (ribonucleic acid) into the mutant zebrafish, the lighter pigment cells become "cured" -- or darker --like those of a normal zebrafish. (
  • In order to explore the role of cpz in zebrafish development, a loss-of-function mutant for zebrafish cpz was generated via TALEN-mediated mutagenesis. (
  • Niccolò Bolli*, Elspeth M. Payne*, Clemens Grabher, Jeong-Soo Lee, Adam B. Johnston, Brunangelo Falini, John P. Kanki and A. Thomas Look Expression of the cytoplasmic NPM1 mutant (NPMc+) causes the expansion of primitive myeloid cells and definitive hematopoietic stem cells in zebrafish Blood 2010 Apr 22;115(16):3329-40. (
  • Zebrafish are also closely related to the genus Devario, as demonstrated by a phylogenetic tree of close species. (
  • The unusually cold temperature was at one of the highest known zebrafish locations at 1,576 m (5,171 ft) above sea level, although the species has been recorded to 1,795 m (5,889 ft). (
  • Zebrafish is a quarterly peer-reviewed journal focusing on research using the zebrafish and related species. (
  • The charity hopes to harness the miraculous healing abilities of zebrafish, a species that is able to mend its own heart muscle. (
  • Given the multitude of (molecular) tools available for the zebrafish, this species promises to be very useful in further elucidating the mechanisms of muscle, skeletal, and cardiovascular adaptations to prolonged exercise. (
  • This first proteomic analysis of the zebrafish skeleton revealed the homology between the zebrafish and the skeleton of other vertebrate species including mammals. (
  • The zebrafish skeleton displays remarkable resemblance to that of other vertebrate species with regards to composition, regulatory components and hormonal response. (
  • The Zebrafish Mutation Project (ZMP) aims to create a knockout allele in every protein--coding gene in the zebrafish genome, using a combination of ENU mutagenesis, whole exome enrichment and Illumina sequencing, in addition to a targeted CRISPR/Cas9 approach. (
  • Zebrafish Mutation Project: Functional Genomics of Disease. (
  • Previously having established methods to generate and identify disruptive zebrafish point mutations on a genome wide scale the Zebrafish Mutation Project is now assigning biological function to every protein-coding gene in the zebrafish genome. (
  • This is really nice work that shows the importance of zebrafish in revealing disease mechanisms related to human mental disorders - in this case, autism," mentioned Su Guo , an associate professor of pharmaceutical sciences at the University of California at San Francisco who´s unaffiliated in the study, in the statement. (
  • Zebrafish epiboly: mechanics and mechanisms. (
  • Recent technological development of exquisite molecular tools in zebrafish, such as cell ablation, lineage analysis, and novel and substantial microarray, together with advancement in stem cell biology, allowed us to investigate how progenitor cells contribute to the generation of appropriate structures and various underlying mechanisms like reprogramming. (
  • Using the antipsychotic drug haloperidol as a test case, we found that behavioral profiles of haloperidol-treated zebrafish could be used to identify previously uncharacterized compounds with desired antipsychotic-like activities and multitarget mechanisms of action. (
  • These findings indicate that zebrafish will be useful for genetically dissecting the molecular mechanisms of cardiac regeneration. (
  • At the 2012 International Zebrafish Development Conference, held June 20-24 in Madison, Wisconsin, numerous presentations highlighted the utility of the zebrafish for examining the basic biological mechanisms underlying human disorders and identifying potential treatment approaches for an impressive array of organ and systemic diseases. (
  • Understanding how zebrafish are able to repair damaged nerves could one day help us to trigger similar mechanisms in human stem cells. (
  • The team uses zebrafish to study the neuronal mechanisms of these heavy metals in connection to neurodegenerative and neuromuscular diseases. (
  • Mechanisms of social buffering of fear in zebrafish. (
  • International Zebrafish Society is an umbrella group that helps to facilitate the exchange of information and resources within the zebrafish community. (
  • The International Zebrafish Society (IZFS) was started in 2015 and IZFS now organizes these meetings. (
  • Zebrafish can also regenerate a severed spinal cord and certain amputated body structures such as scales and fins. (
  • Here, we demonstrate histologically that zebrafish fully regenerate hearts within 2 months of 20% ventricular resection. (
  • The hearts of zebrafish with mutations in the Mps1 mitotic checkpoint kinase, a critical cell cycle regulator, failed to regenerate and formed scars. (
  • To determine whether zebrafish can also regenerate heart muscle, we surgically removed ∼20% of the ventricular myocardium from 1- to 2-year-old adults ( Fig. 1 , A and B) and examined hearts histologically at eight time points after surgery ( 5 ). (
  • Zebrafish Can Regenerate Their Spinal Cords, What about People? (
  • Mammals cannot regenerate their complex nervous tissue but small, tropical zebrafish can repair a severed spine in eight weeks. (
  • However, a small, tropical freshwater fish native to South East Asia called zebrafish has the ability to rapidly regenerate their nervous tissue. (
  • Information in ZFIN is tightly linked to the web resources of the Zebrafish International Resource Center ( ZIRC ), the China Zebrafish Resource Center ( CZRC ), and so on, which maintain and provide zebrafish-related research resources, materials and services. (
  • ZIRC is the Zebrafish International Resource Centre-A stock centre, holding the various strains of zebrafish centrally. (
  • Zebrafish: Methods and Protocols, Second Edition guides readers through methods for mutagenesis and genome editing in zebrafish, applications of GFP-expressing transgenic fish, techniques for cancer models, imaging of infection and host-pathogen interactions, metabolism and transport of lipids, and the structure and function of neural circuits and their role in generating behavior. (
  • Paternal exposure to a common herbicide alters the behavior and serotonergic system of zebrafish offspring. (
  • Following that trend, these zebrafish also showed increasingly uncontrolled behavior (meander) with increased doses. (
  • Being social animals, zebrafish are an interesting model for studying psychiatric disorders that affect social behavior, such as schizophrenia and autism. (
  • Shoaling is a straightforward expression of social behavior in zebrafish. (
  • The study of zebrafish behavior can include a lot of parameters and assessing these is a lot easier with the right software. (
  • Scientific Reports, 2017 ) tracked freezing behavior and erratic movements of zebrafish in detail, and found that zebrafish handled an aversive stimulus better if they are not alone. (
  • From these tracks detailed analysis of freezing behavior and erratic movements was performed to assess fear behavior in the zebrafish. (
  • Washington, Sep 5 (IANS) Loud noise is known to irreversibly damage hair cells within the inner ear of mammals and cause deafness, but the ability of zebrafish to regrow these cells holds hope, new research says. (
  • Together, these studies suggest that the cellular constituents responsible for antigen presentation are remarkably conserved from teleosts to mammals, and indicate that the zebrafish may serve as a unique model to study the origin of APC subsets and their evolutionary role as the link between the innate and adaptive immune systems. (
  • Here, we have addressed the biology of DCs in the zebrafish, a small cyprinid teleost that features an immune system similar to that of mammals ( 11 ). (
  • The question of whether the regenerative powers of zebrafish and salamanders represent ancient abilities that mammals have lost, perhaps in exchange for advanced tumor-suppression systems remains an open question for biologists. (
  • 7 18 19 The earliest site of zebrafish hematopoiesis is the ICM, a region analogous to the blood islands of the yolk sac in mammals. (
  • Using a technique developed by Lichtman's laboratory, he cut the front quarter of the zebrafish larva-a total length of 1 millimeter, or about 4 hundredths of an inch-into more than 18,000 slices. (
  • In 2009, Kanungo set up the zebrafish laboratory at NCTR. (
  • This volume of Methods in Cell Biology , the second of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. (
  • Due to their small size, large numbers of zebrafish can be housed in the laboratory at low cost. (
  • Additionally, the presentation will include information on how specific zebrafish strains are tracked in a laboratory setting, with specific reference to the collection of tail fin samples for DNA extraction and genotyping. (
  • 5-9 Chemical mutagenesis screens in this organism have resulted in the isolation of numerous zebrafish lines containing mutations that affect both embryonic and definitive erythropoiesis. (
  • Zebrafish can develop a form of the disease genetically similar to human skin cancer tumors. (
  • Furthermore, zebrafish cancers are similar to human tumors at the histological and molecular levels allowing the study of tumor initiation, progression and heterogeneity. (
  • SALT LAKE CITY, Dec. 24 (UPI) -- A study of zebrafish has revealed ways to potentially slow or prevent the onset of cancerous tumors, the Huntsman Cancer Institute in Utah says. (
  • The zebrafish, a translucent fish often used as a model of human development and disease, offers unique advantages for studying the cause, growth, and spread of tumors using strategies and methods presented in the current "Cancer Biology" special issue of Zebrafish, a peer-reviewed journal published by Mary Ann Liebert, Inc. ( ). (
  • A particular advantage of using zebrafish to study cancer biology is the ability to transplant human tumors into the fish using well-established methods. (
  • Zebrafish larvae transplanted with patients' tumors respond as their human donors do to chemotherapy. (
  • 8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element (Koichi Kawakami)9. (
  • The zebrafish pronephric kidney provides a simple, yet powerful, model system to better understand the conserved molecular and cellular progresses that drive nephron formation, structure, and function. (
  • Here, we review work on the molecular and cellular basis of zebrafish epiboly in order to identify unifying themes and to highlight some of the current open questions. (
  • Sensory neurons in the tip of the zebrafish nose respond to molecular signals released from food sources. (
  • Thus, the mpo gene should provide a useful molecular probe for identifying zebrafish mutants with defects in granulopoiesis. (
  • Our analysis of zebrafish forebrain mutants led to the discovery that the small protein Sonic Hedgehog (Shh) is a critical player in the early induction of the pituitary placode, as well as in the differentiation of a subset of endocrine cell types. (
  • A , Schematic of zebrafish NOX protein domains as determined by alignment to the human protein sequences. (
  • The human CTGF protein is nearly 90% similar to that of the zebrafish. (
  • By determination of the protein content in the zebrafish skeletal ECM with major changes in protein abundance during development we were able to identify various components of signaling pathways implicated in skeletogenesis. (
  • In this thesis we provide a first insight in the extracellular protein content of the zebrafish skeleton, identify a role for the Cpz protein in β-catenin independent (non-canonical) Wnt signaling during development and shows the effect of thyroid hormone on ossification during early zebrafish development. (
  • Zebrafish models are also amenable to high-throughput chemical compound screening, thereby enabling discovery of novel drug compounds. (
  • These advantages have led to an upward trend in high-throughput zebrafish chemical screens, especially toward screens of many chemicals using large quantities of fish (Usenko et al. (
  • As small vertebrate animals, zebrafish are amenable to high throughput screening and require only small amounts of compounds in testing scenarios. (
  • Genetically engineered zebrafish could provide a quick way to test new drugs to prevent dangerous plaque buildup in the heart. (
  • In the last 10 to 15 years, as regenerative organisms like zebrafish have become genetically tractable to study in the lab, I became convinced that these animals might be able to teach us what is possible for human regeneration," Pomerantz said. (
  • Zebrafish floating head ( flh ) mutants lack a notochord, but develop with prechordal plate and other mesodermal derivatives, indicating that flh functions specifically in notochord development. (
  • Zebrafish reward mutants reveal novel tra. (
  • These marvelous mutants, and others, are under the charge of Hui Feng, director of BU's Lab of Zebrafish Genetics and Cancer Therapeutics , and they may hold clues to treating breast cancer. (
  • This second edition details new emerging areas of zebrafish research focusing on genetics and genomics, techniques for developing and analyzing zebrafish disease models, and methods for neuroscience. (
  • Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. (
  • a relational database containing biological data that are curated from the scientific literature and that are directly submitted by zebrafish research laboratories (e.g. (
  • Zebrafish is being used in Roche research and early development for both safety and efficacy studies. (
  • Roche is at the head of the class when it comes to the school of zebrafish research, and is pursuing the use of zebrafish in both safety and efficacy testing. (
  • Yet, following this humble beginning, why has the zebrafish developed into one of the most popular model organisms for research? (
  • Instead, a combination of characteristics makes the zebrafish so attractive that countless labs around the world now use it for research: in the Max Planck Society alone at seven different Institutes. (
  • From the processes during embryonal development up to information processing in the brain - the zebrafish is a popular model organisms in basic research. (
  • The zebrafish is an important and widely used vertebrate model organism in scientific research, for example in drug development, in particular pre-clinical development. (
  • Although cell culture and mouse models will remain a cornerstone of cancer research, the unique capabilities of the zebrafish outline the potential of this model for shedding light on cancer biology in vivo. (
  • Here, we review different involvements of the zebrafish model in cancer research. (
  • Zebrafish-so named for the speckled "stripes" along its side and originally found in the Ganges River in East India and Burma-have been making a splash in the world of scientific research for some time. (
  • The research showed that growth hormone is involved in this regeneration in zebrafish. (
  • ZEBRAFISH, little freshwater tropical fish that populate pet-store aquariums, are making a splash in several arenas of drug discovery research. (
  • Our research group studies lymphatic vessel formation in zebrafish, as a main model system. (
  • Newswise - BETHESDA, MD - July 6, 2012 - Zebrafish, popular as aquarium fish, now have an important place in research labs as a model organism for studying human diseases. (
  • Dr Caroline Brennan (QMUL) explains why zebrafish make excellent research models. (
  • Find out about grants awarded to projects applying the 3Rs in zebrafish research. (
  • The following links provide additional information on zebrafish and their role in research. (
  • Fish for Pharma presents a short summary of why zebrafish make an ideal animal model for research. (
  • Request a free demo for your zebrafish research. (
  • Watch the movie about zebrafish research at a glance and request a free demo! (
  • The use of zebrafish as a model in future research will undoubtedly increase our understanding of vertebrate skeletal development and disease. (
  • The Zebrafish Conference on Development and Genetics allows attendees to network with premier Zebrafish geneticists, forge new collaborations, and get updated on cutting-edge research in a diverse array of topics. (
  • Zebrafish Model Organism Database. (
  • The zebrafish is a relatively new model organism in the field of skeletal development and primarily used as a powerful model for the identification of novel gene functions during skeletogenesis. (
  • The zebrafish is a derived member of the genus Brachydanio, of the family Cyprinidae. (
  • This unique model is the only way to visualise these cells during inflammation, and given the small size of zebrafish, makes drug screens a practical prospect using this approach. (
  • This success is only the first of a range of zebrafish drug screens, which I am hopeful will provide real benefits to health in the coming years. (
  • Zebrafish are especially amenable to phenotype-based drug discovery (PDD) screens. (
  • In fact, PDD screens performed on zebrafish have led to the identification of some lead compounds of therapeutic potential. (
  • Moreover, whether zebrafish larvae display long-term habituation, and thus the ability for memory storage and retrieval, is unclear. (
  • Ann Morris, a 2011 Pew Biomedical Scholar and assistant professor at the University of Kentucky, didn't know as a child that she would study zebrafish for clues on improving human vision, but she did know that one day she'd become a scientist. (
  • Being a vertebrate, Zebrafish has blood, eye, heart, kidney and other biological processes that share many features of the human system. (
  • In the 1970s, then-UO professor George Streisinger discovered that zebrafish made an excellent model for humanlike biological processes. (
  • A zebrafish can even regrow parts of its heart. (
  • The zebrafish is an alternative in vivo model which is currently being evaluated at Roche to study the effects of compounds on target organ toxicities. (
  • Zebrafish are almost transparent during the early stages of life, allowing the in vivo observation of internal organs and tissues. (
  • We took advantage of this experimental system to perform in vivo loss and gain of function analysis of candidate receptors of the class II helical receptor family and identified zCRFB1 and zCRFB5 as the two subunits of the zebrafish IFN receptor. (
  • The zebrafish embryo develops rapidly, with precursors to all major organs appearing within 36 hours of fertilization. (
  • Zebrafish is an excellent animal model, which can repair several organs like damaged retina, severed spinal cord, injured brain and heart, and amputated fins. (
  • Chen YH, Chang CF, Lai YY, Sun CY, Ding YJ, Tsai JN (2015) von Hippel-Lindau gene plays a role during zebrafish pronephros development. (
  • This intensive two-week course for advanced graduate students, postdoctoral fellows, and independent investigators will focus on the development and genetics of zebrafish. (
  • Zebrafish Wild Type Strain Genome was presented at the 6th European Zebrafish Genetics and Development Meeting at Rome, Italy. (
  • The Zebrafish Community meets biannually at the ' International Conference on Zebrafish Development and Genetics ', which usually takes place in Madison/Wisconsin, USA. (
  • The biannual European Zebrafish Genetics and Development Meeting alternates with the International Conference and takes place at changing locations throughout Europe. (
  • Zebrafish are useful for studying how left-right brain asymmetries arise during embryonic development and how the resultant asymmetric neuronal circuitry ultimately impacts on function and behaviour. (
  • Zebrafish are an excellent vertebrate model for investigating normal and abnormal development. (
  • This elaborate but easy-to use website details the complete vascular anatomy of the zebrafish embryo and larva at every stage of development from 1 to 7 days post-fertilization. (
  • Our lab has previously reported the development of a reliable conditioned place preference paradigm for zebrafish. (
  • Ontology term enrichment analysis on gene ontology (GO) annotations combined with the zebrafish anatomical and development (ZFA) ontology has led to surprisingly detailed insights into phenotypes. (
  • This chapter reviews the effects of exercise on the development of the musculoskeletal system, the cardiovascular system, metabolic capacities of developing zebrafish, and regulation of these processes on the gene expression level. (
  • Such restoration of ERK1/2 activation was sufficient to restore impaired arterial development and branching morphogenesis in synectin-deficient mice and synectin-knockdown zebrafish. (
  • Thyroid hormones are required for skeletal development (Kim and Mohan, 2013), but to what extent thyroid hormone affects early bone development in zebrafish remained unclear. (
  • This provides the first histological evidence of increased ossification in zebrafish due to thyroid hormone exposure which can be used as a starting point to explore the mechanism of thyroid hormone on skeletal development at a greater depth. (
  • We used morpholino knockdown in zebrafish to define the role of mig6 in both exocrine pancreas and islet development. (
  • As a zebrafish embryo develops, the cells that will form its lateral line have to become pointed at one end and arrange themselves around a central, unchanged cell, like cloves in a bulb of garlic, forming a rosette. (
  • Utilising the proprietary phenotype-based zebrafish model for Dravet Syndrome, drug candidates were identified from a screen of more than 3,000 drugs that suppress seizures and symptoms associated with neurological diseases. (
  • Paired-end reads were collected on an Illumina 3000HT, then aligned to the most recent zebrafish reference genome (GRCz10). (
  • The zebrafish reference genome sequence and its relationship to the human genome. (
  • [2] To facilitate use of the zebrafish as a model of human biology, ZFIN links these data to corresponding information about other model organisms (e.g., mouse ) and to human disease databases. (
  • Zebrafish Resources can be found at the central website of the zebrafish community at ZFIN . (
  • Standard toxicity experiments carried out in typical cell culture experiments lack the complexity found in small model organisms such as zebrafish. (
  • Traditionally, vertebrate model organisms such as mouse and zebrafish have been used on a gene-by-gene basis, however, in order to keep pace with the increasing speed of discovery, new approaches are needed. (
  • Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. (
  • We extensively analyzed palatogenesis in zebrafish to determine the similarity of gene expression and function across vertebrates. (
  • Zebrafish were also among the first vertebrates to be cloned (frogs were cloned decades earlier). (
  • Burckle C, Gaude HM, Vesque C, Silbermann F, Salomon R, Jeanpierre C, Antignac C, Saunier S, Schneider-Maunoury S (2011) Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros. (
  • Role of microtubules in morphogenesis of the zebrafish embryo. (
  • Ensemble's Zebrafish Genome Browser is a central genome database, which offers information free online. (
  • Pain, sedation, tumor metastasis, vascular tone, and gut motility are all examples of disease-relevant phenotypes that are observable in zebrafish yet inaccessible by modeling in cultured cells. (
  • To generate image datasets containing all the nerve cells in the zebrafish brain and their many intricate connections, then-graduate-student Hildebrand had to dig deeper than the previous studies. (
  • for blood cells of zebrafish. (
  • Early studies in zebrafish led to the idea that dying retinal cells release signals that trigger support cells in the retinal called Muller glia to dedifferentiate -return to a stem-like state - and proliferate. (
  • They are hoping the zebrafish study will help them fill in the gaps by showing them how and where CD46 controls the movement of immune cells into the brain. (
  • 2 Zebrafish larvae, unlike cells in a dish, possess functional livers, kidneys, and blood-brain barriers, and thus, drugs can be tested in a complete system. (
  • Because teleosts possess one of the earliest recognizable adaptive immune systems, we sought to identify antigen-presenting cells (APCs) in the zebrafish to better understand the potential origins of DCs and their evolutionary relationship to lymphocytes. (
  • This fluttering clump of colorful cells is a zebrafish embryo, visualized by many stacked images. (
  • Although it might seem odd to study breast cancer in an animal with no breasts, zebrafish do have lungs, hearts, brains, eyes, and blood vessels that respond to cancer cells much as human vasculature does. (
  • Feng's strategy is to combine observations and experiments in zebrafish with tests in human cells and analysis of human cancer genome databases. (
  • Granulocytic cells expressing zebrafish mpo were first evident at 18 hours after fertilization (hpf) in the posterior intermediate cell mass (ICM) and on the anterior yolk sac by 20 hpf. (
  • 16 17 By contrast, primitive hematopoiesis in the zebrafish occurs within the embryo in a region between the notochord and endoderm of the trunk called the intermediate cell mass (ICM), as well as anteriorly in the paraxial mesoderm over the yolk cell and posteriorly in a small ventral cluster of cells in the developing tail referred to as the posterior blood island. (
  • Remarkably, however, zebrafish can heal themselves from spinal cord injury by growing new motor neurons from stem cells present in the spinal cord. (
  • When the spinal cord of the zebrafish is struck, glial cells build a bridge across the severed tissues of the spinal cord. (
  • Neogenic β cells may arise from two sources in the developing zebrafish, which can be distinguished by the retention of a fluorescent mark made at the one-cell stage. (
  • When a zebrafish is injured, a bridge of supporting glial cells forms between the two ends. (
  • Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish," Mayssa H. Mokalled, Chinmoy Patra, Amy L. Dickson, Toyokazu Endo, Didier Y. R. Stainier, Kenneth D. Poss. (