A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Proteins found in any species of fungus.
The functional hereditary units of FUNGI.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.
A genus of ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Structures within the nucleus of fungal cells consisting of or containing DNA, which carry genetic information essential to the cell.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The complete gene complement contained in a set of chromosomes in a fungus.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Reproductive bodies produced by fungi.
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
An order of fungi in the phylum Ascomycota that multiply by budding. They include the telomorphic ascomycetous yeasts which are found in a very wide range of habitats.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.
The chromosomal constitution of cells, in which each type of CHROMOSOME is represented once. Symbol: N.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
Fermented juice of fresh grapes or of other fruit or plant products used as a beverage.
The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.
A glycoside hydrolase found primarily in PLANTS and YEASTS. It has specificity for beta-D-fructofuranosides such as SUCROSE.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The rate dynamics in chemical or physical systems.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Genes that have a suppressor allele or suppressor mutation (SUPPRESSION, GENETIC) which cancels the effect of a previous mutation, enabling the wild-type phenotype to be maintained or partially restored. For example, amber suppressors cancel the effect of an AMBER NONSENSE MUTATION.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Protein factors released from one species of YEAST that are selectively toxic to another species of yeast.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A set of nuclear proteins in SACCHAROMYCES CEREVISIAE that are required for the transcriptional repression of the silent mating type loci. They mediate the formation of silenced CHROMATIN and repress both transcription and recombination at other loci as well. They are comprised of 4 non-homologous, interacting proteins, Sir1p, Sir2p, Sir3p, and Sir4p. Sir2p, an NAD-dependent HISTONE DEACETYLASE, is the founding member of the family of SIRTUINS.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Proteins prepared by recombinant DNA technology.
A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).
An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Transport proteins that carry specific substances in the blood or across cell membranes.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Fungal genes that mostly encode TRANSCRIPTION FACTORS. In some FUNGI they also encode PHEROMONES and PHEROMONE RECEPTORS. The transcription factors control expression of specific proteins that give a cell its mating identity. Opposite mating type identities are required for mating.
A steroid of interest both because its biosynthesis in FUNGI is a target of ANTIFUNGAL AGENTS, notably AZOLES, and because when it is present in SKIN of animals, ULTRAVIOLET RAYS break a bond to result in ERGOCALCIFEROL.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Deletion of sequences of nucleic acids from the genetic material of an individual.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A protein kinase encoded by the Saccharomyces cerevisiae CDC28 gene and required for progression from the G1 PHASE to the S PHASE in the CELL CYCLE.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The ability of fungi to resist or to become tolerant to chemotherapeutic agents, antifungal agents, or antibiotics. This resistance may be acquired through gene mutation.
Genes whose loss of function or gain of function MUTATION leads to the death of the carrier prior to maturity. They may be essential genes (GENES, ESSENTIAL) required for viability, or genes which cause a block of function of an essential gene at a time when the essential gene function is required for viability.
Any method used for determining the location of and relative distances between genes on a chromosome.
The process by which a DNA molecule is duplicated.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
Organisms whose GENOME has been changed by a GENETIC ENGINEERING technique.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.
Chemical substances, excreted by an organism into the environment, that elicit behavioral or physiological responses from other organisms of the same species. Perception of these chemical signals may be olfactory or by contact.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
A sirtuin family member found primarily in the CYTOPLASM. It is a multifunctional enzyme that contains a NAD-dependent deacetylase activity that is specific for HISTONES and a mono-ADP-ribosyltransferase activity.
A carboxypeptidase that catalyzes the release of a C-terminal amino acid with a broad specificity. It also plays a role in the LYSOSOMES by protecting BETA-GALACTOSIDASE and NEURAMINIDASE from degradation. It was formerly classified as EC and EC
Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC, EC, EC, and EC
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
An enzyme that converts UDP glucosamine into chitin and UDP. EC
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A DNA-binding protein that mediates DNA REPAIR of double strand breaks, and HOMOLOGOUS RECOMBINATION.
A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.
The sum of the weight of all the atoms in a molecule.
Toxic compounds produced by FUNGI.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.
An isomer of glucose that has traditionally been considered to be a B vitamin although it has an uncertain status as a vitamin and a deficiency syndrome has not been identified in man. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1379) Inositol phospholipids are important in signal transduction.
A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS from SACCHAROMYCES CEREVISIAE. It is involved in morphological events related to the cell cycle. This enzyme was formerly listed as EC
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.
Methods and techniques used to genetically modify cells' biosynthetic product output and develop conditions for growing the cells as BIOREACTORS.
Life or metabolic reactions occurring in an environment containing oxygen.
A genus of yeast-like mitosporic Saccharomycetales fungi characterized by producing yeast cells, mycelia, pseudomycelia, and blastophores. It is commonly part of the normal flora of the skin, mouth, intestinal tract, and vagina, but can cause a variety of infections, including CANDIDIASIS; ONYCHOMYCOSIS; vulvovaginal candidiasis (CANDIDIASIS, VULVOVAGINAL), and thrush (see CANDIDIASIS, ORAL). (From Dorland, 28th ed)
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.
The relationships of groups of organisms as reflected by their genetic makeup.
A urea hydantoin that is found in URINE and PLANTS and is used in dermatological preparations.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
An alkylating agent in cancer therapy that may also act as a mutagen by interfering with and causing damage to DNA.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
The aggregation of suspended solids into larger clumps.
Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.
A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.
Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.
A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6.
The dry cells of any suitable strain of SACCHAROMYCES CEREVISIAE or CANDIDA. It can be obtained as a by-product from the brewing of beer or by growing on media not suitable for beer production. Dried yeast serves as a source of protein and VITAMIN B COMPLEX.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
Elements of limited time intervals, contributing to particular results or situations.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
An enzyme that catalyzes the conversion of alpha,alpha-trehalose and water to D-glucose. EC
A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum.
A systemic agricultural fungicide used for control of certain fungal diseases of stone fruit.
Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987)
A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A class of enzymes that form a thioester bond to UBIQUITIN with the assistance of UBIQUITIN-ACTIVATING ENZYMES. They transfer ubiquitin to the LYSINE of a substrate protein with the assistance of UBIQUITIN-PROTEIN LIGASES.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
An enzyme that catalyzes reversibly the formation of galactose 1-phosphate and ADP from ATP and D-galactose. Galactosamine can also act as the acceptor. A deficiency of this enzyme results in GALACTOSEMIA. EC
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Product of the oxidation of ethanol and of the destructive distillation of wood. It is used locally, occasionally internally, as a counterirritant and also as a reagent. (Stedman, 26th ed)
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A family of pheromone receptors that were initially discovered in SACCHAROMYCES CEREVISIAE as proteins necessary for fungal conjugation. Each mating factor receptor is expressed in HAPLOID CELLS of a single mating type.
A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.
The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Comparative analysis between Torulaspora delbrueckii and Saccharomyces cerevisiae baker's yeast strains". Antonie van ... "Yeast communities and genetic polymorphism of Saccharomyces cerevisiae strains associated with artisanal fermentation in Brazil ... Torulaspora delbrueckii was formerly known as Saccharomyces delbrueckii or Saccharomyces rosei or Saccharomyces roseus, and the ... Torulaspora delbrueckii is a ubiquitous yeast species with both wild and anthropic habitats. The type strain of T. delbrueckii ...
Gardasil's proteins are synthesized by the yeast Saccharomyces cerevisiae. Its protein makeup allows it to target four types of ... which covers 4 major HPV strains, with an updated vaccine providing protection from 9 strains. The over cost with the "gender- ... Patients with a hypersensitivity to yeast should not receive Gardasil since yeast is used in its production. ... Gardasil contains inactive L1 proteins from four different HPV strains: 6, 11, 16, and 18. Each vaccine dose contains 225 µg of ...
... mutants using Saccharomyces cerevisiae". FEMS Yeast Research. 9 (8): 1226-35. doi:10.1111/j.1567-1364.2009.00581.x. PMID ... "WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta strain in the presence of WIP". Biochemical and ... In addition to a role in endocytosis, Saccharomyces cerevisiae Vrp1 functions in cytokinesis and cell polarization. In ... WIPF1 functions and interactions have been studied in multiple fungal systems including Saccharomyces cerevisiae, ...
... mutants using Saccharomyces cerevisiae". FEMS Yeast Research. 9 (8): 1226-35. doi:10.1111/j.1567-1364.2009.00581.x. PMID ... "WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta strain in the presence of WIP". Biochemical and ...
... is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain is sold ... Guo, Deyin; Rajamäki, Minna-Liisa; Valkonen, Jari (2008). "Protein-Protein Interactions: The Yeast Two-Hybrid System". Plant ... commercially by Clontech and is used as a partner with strain Y187 in mating assays. DeFrancesco, Laura; Wilkinson, Deborah ( ...
"IDI1 - Isopentenyl-diphosphate Delta-isomerase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - IDI1 ... including the yeast Saccharomyces cerevisiae, the nematode Caenorhabditis elegans and the plant Arabidopsis thaliana. While ... the gene for isopentenyl diphosphate isomerase in Saccharomyces cerevisiae". Yeast. 8 (9): 743-748. doi:10.1002/yea.320080907. ... strain CL190". Proc. Natl. Acad. Sci. U.S.A. 98 (3): 932-7. doi:10.1073/pnas.020472198. PMC 14687. PMID 11158573. Bishop JM ( ...
... is a strain of yeast (Saccharomyces cerevisiae) used in biological research for two-hybrid screening. The strain has been ... Fromont-Racine, Micheline; Rain, Jean-Christophe; Legrain, Pierre (1997). "Toward a functional analysis of the yeast genome ... Guo, Deyin; Rajamäki, Minna-Liisa; Valkonen, Jari (2008). "Protein-Protein Interactions: The Yeast Two-Hybrid System". Plant ... sold commercially by Clontech since at least 2000 and is used as a partner with strain AH109 in mating assays. Genotype of Y187 ...
... yeast Saccharomyces cerevisiae, chromosomal rearrangements are a major mechanism to reproductively isolate different strains. ... "Chromosomal Rearrangements as a Major Mechanism in the Onset of Reproductive Isolation in Saccharomyces cerevisiae". Current ... These authors crossed 60 natural isolates sampled from diverse niches with the reference strain S288c and identified 16 cases ... Two wasp species Nasonia giraulti and N. longicornis carry two different strains of Wolbachia. Crosses between an infected ...
... farnesene is produced from fermentation of sugarcane sugars using genetically modified Saccharomyces cerevisiae yeast strains. ...
... of Chromosome 13 has been observed in the Flor strains of the yeast species Saccharomyces cerevisiae. Chromosome 13 ... "Chromosome specificity of polysomy promotion by disruptions of the Saccharomyces cerevisiae RNA1 gene". Genetics. 116 (3): 371- ... Polysomy of Chromosome 13 is promoted when there is disruption of the yeast RNA1 gene with LEU2 sequences. Fluorescence in situ ...
... a unique hybrid of Saccharomyces cerevisiae yeast was found on fallen cherries near Fish Creek. This strain of S. cerevisiae ... "A unique ecological niche fosters hybridization of oak-tree and vineyard isolates of Saccharomyces cerevisiae". Molecular ...
introduced a monoterpene synthase from sweet basil into Saccharomyces cerevisiae, a strain of yeast. This particular ... Oswald, M.; Fischer, M.; Dirninger, N.; Karst, F. (2007). "Monoterpenoid biosynthesis inSaccharomyces cerevisiae". FEMS Yeast ... especially for strains of algae that are susceptible to these metals. In open pond systems the use of strains of algae that can ... 3,000 algal strains were collected from around the country and screened for desirable properties such as high productivity, ...
"GOS1 - Golgi SNAP receptor complex member 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - GOS1 gene ... "TAE1 - Alpha N-terminal protein methyltransferase 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - ... were found to interact with TMEM239 through a host-pathogen yeast two hybrid screen. Additional TMEM239 protein interactions ...
Herskowitz I (December 1988). "Life cycle of the budding yeast Saccharomyces cerevisiae". Microbiological Reviews. 52 (4): 536- ... analyzed the ancestry of natural S. cerevisiae strains and concluded that outcrossing occurs only about once every 50,000 cell ... Saccharomyces cerevisiae, brewer's and baker's yeast, is in the phylum Ascomycota. During vegetative growth that ordinarily ... Thus, although S. cerevisiae is heterothallic, it appears that, in nature, mating is most often between closely related yeast ...
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)". Uniprot. Uniprot Consortium. Retrieved 13 August 2014. ... strain ATCC 204508/S288c. It is found on the gene UAF30. "RNA polymerase I upstream activating factor complex". Saccharomyces ... is a protein found in baker's yeast, ...
... signaling in Saccharomyces cerevisiae (yeast). "VIB and Performa Investimentos establish GlobalYeast to deliver superior ... industrial yeast strains for the bio based economy". "GlobalYeast - Smart Fermentation". Van Nuland A, Vandormael P, Donaton M ... a spin of company that will develop and deliver superior industrial strains. His research interest is on the molecular genetics ... Ammonium permease-based sensing mechanism for rapid ammonium activation of the protein kinase A pathway in yeast, Mol Microbiol ...
... fermenting yeasts such as Scheffersomyces Pichia stipitis or by metabolically engineered strains of Saccharomyces cerevisiae. ... Pichia stipitis is not as ethanol tolerant as the traditional ethanol producing yeast Saccharomyces cerevisiae. S. cerevisiae ... 2002). "Reduced Oxidative Pentose Phosphate Pathway Flux in Recombinant Xylose-Utilizing Saccharomyces cerevisiae Strains ... the key to efficient ethanolic fermentation of xylose by Saccharomyces cerevisiae? ;;FEMS Yeast Res.;; 2003 Oct; 4(1) 69-78. ...
For example, mutagenized yeast strains cannot be used for further genetic analysis such as tetrad analysis. Markers would have ... In Saccharomyces cerevisiae, the RAD52 gene is essential for homologous recombination, and thus is required for the delitto ... In addition, human genes can be studied and similarly genetically manipulated in yeast by using yeast artificial chromosomes ( ... CORE-containing yeast cells are transformed with oligonucleotides containing the desired mutation such that they lead to the ...
Many of these are not strains of brewer's yeast (Saccharomyces cerevisiae) and may have significant differences in aroma and ... Top-fermented beers are most commonly produced with Saccharomyces cerevisiae, a top-fermenting yeast which clumps and rises to ... The dominant types of yeast used to make beer are the top-fermenting Saccharomyces cerevisiae and bottom-fermenting ... Lager yeast is a cool bottom-fermenting yeast (Saccharomyces pastorianus) and typically undergoes primary fermentation at 7-12 ...
... combing of DNA fibers can be used to monitor the structure of chromosomes in the budding yeast Saccharomyces cerevisiae. This ... This requires that the yeast strains express thymidine kinase, which wild type yeasts do not, being fungi (see occurrence). ... Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N (July 2004). "In vivo labeling of fission yeast DNA with thymidine and ... Gallo D, Wang G, Yip CM, Brown GW (February 2016). "Analysis of Replicating Yeast Chromosomes by DNA Combing". Cold Spring ...
Systems similar to Ames test have been developed in yeast. Saccharomyces cerevisiae is generally used. These systems can check ... Sex-Linked Recessive Lethal Test - Males from a strain with yellow bodies are used in this test. The gene for the yellow body ... DNA repair - E. coli and Bacillus subtilis strains deficient in DNA repair may be used to detect mutagens by their effect on ... Transgenic mouse assay using a mouse strain infected with a viral shuttle vector is another method for testing mutagens. ...
"RRM3 - ATP-dependent DNA helicase RRM3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - RRM3 gene & ... "Yeast two-hybrid analysis of the origin recognition complex of Saccharomyces cerevisiae: interaction between subunits and ... "RRM3 Rrm3p [Saccharomyces cerevisiae S288C] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2017-11-25. Torres, Jorge Z.; ... Rrm3p is one of many helicase proteins in Saccharomyces cerevisiae. Rrm3p a DNA helicase that unwinds DNA in a 5'-to-3' ...
... synthesized in the yeast Saccharomyces cerevisiae. Each vaccine dose contains 225 µg of aluminum, 9.56 mg of sodium chloride, ... Gardasil 9 protects against infection from the strains covered by the first generation of Gardasil (HPV-6, HPV-11, HPV-16, and ... Patients with a hypersensitivity to yeast should not receive Gardasil since yeast is used in its production. People with ... Additionally, HIQA is aiming to replace the current vaccination, which covers 4 major HPV strains, with an updated vaccine ...
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - ILV2 gene & protein". www.uniprot.org. Dailey FE, ... In baker's yeast, they are located in the mitochondria. In several experiments, it has been shown that mutated strains of ... The hetromeric structure was demonstrated in E. coli in 1984 and in eukaryotes (S. cerevisiae and Porphyra purpurea) in 1997. ...
For example, Saccharomyces cerevisiae strain ML01 (S. cerevisiae strain ML01), which carries a gene encoding malolactic enzyme ... The cultured yeasts most commonly used in winemaking belong to the Saccharomyces cerevisiae (also known as "sugar yeast") ... Alternative, non-Saccharomyces cerevisiae, yeasts are being used more prevalently in the industry to add greater complexity to ... few yeast strains are actively involved in the fermentation process. The use of active dry yeasts reduces the variety of ...
... which makes them diverse among other Saccharomyces strains. The yeast species Schizosaccharomyces pombe and Saccharomyces ... Forsburg SL (June 2005). "The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe: models for cell biology research ... Fission yeast does not have as many duplicated genes compared to budding yeast, only containing 5%, making fission yeast a ... cerevisiae. Since most strains of fission yeast were isolated from brewed beverages, there is no ecological or historical ...
Lethal concentrations of puromycin are much higher for strains of Saccharomyces cerevisiae than mammalian cell lines. Deletion ... Selection of yeast[edit]. Puromycin resistance in yeast can also be conferred through expression of the puromycin N-acetyl- ... But use of puromycin for E. coli selection requires precise pH adjustment and also depends on which strain is selected. For ... "Puromycin- and methotrexate-resistance cassettes and optimized Cre-recombinase expression plasmids for use in yeast". Yeast ...
In 1965, Brian Cox, a geneticist working with the yeast Saccharomyces cerevisiae, described a genetic trait (termed [PSI+]) ... When the strain is grown on yeast-extract/dextrose/peptone media (YPD), the blocked pathway results in buildup of a red-colored ... Several prion-forming proteins have been identified in fungi, primarily in the yeast Saccharomyces cerevisiae. These fungal ... Wickner RB (1994). "[URE3] as an altered URE2 protein: evidence for a prion analog in Saccharomyces cerevisiae". Science. 264 ( ...
The type of yeast most commonly found in tequila is Saccharomyces cerevisiae, which contains many strains.[citation needed] For ... The two main categories of yeast used in tequila are commercial brewers yeast and yeast that comes from precultivated existing ... One of the most abundant esters is ethyl acetate which is synthesized during fermentation by the yeast Saccharomyces cerevisiae ... The alcohol content in tequila is affected by three factors: the amount of isoamyl alcohol and isobutanol in the yeast strain, ...
... and testing for revertants of a tryptophan auxotroph in Saccharomyces cerevisiae (yeast). The E. coli strain FC40 has a high ... specifically Saccharomyces cerevisiae. He tested for tryptophan auxotroph revertants. A tryptophan auxotroph cannot make ... He found that when yeast colonies were moved from a tryptophan-rich medium to a minimal one, revertants continued to appear for ... The degree to which revertants were observed in yeast was not as high as with bacteria. Other scientists have conducted similar ...
... cerevisiae.[4] Studies, however, deem it to be crabtree positive which is likely due to strain differences since K. marxianus ... the yeast cells appear globose, ellipsoidal or cylindrical, 2-6 x 3-11 μm in size.[6] In a glucose-yeast extract broth, K. ... This species was first described in the genus Saccharomyces as S. marxianus by the Danish mycologist, Emil Christian Hansen ... Kluyveromyces marxianus in ascomycetous yeast and member of the genus, Kluyveromyces. It is the sexual stage of ...
In budding yeast, Saccharomyces cerevisiae, the paralogs Rad55 and Rad57 are present, which form a complex that associates with ... doi:10.1016/j.str.2006.04.001. PMID 16765891.. *^ a b c Buisson R, Dion-Côté AM, Coulombe Y, Launay H, Cai H, Stasiak AZ, ... RAD51 family members are homologous to the bacterial RecA, Archaeal RadA and yeast Rad51.[5][6] The protein is highly conserved ... Shinohara A, Ogawa H, Ogawa T (May 1992). "Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like ...
The Institute has contributed to genome-sequencing projects of the common yeast (Saccharomyces cerevisiae, an organism which ... Strains of bacteria and viruses from many different countries are sent to the Institute's reference center for identification. ... While Pasteur believed that the only substance implied in the process of fermentation was yeast, Bernard - and Berthelot in his ...
Knockout or null mutations in SOD1 are highly detrimental to aerobic growth in the budding yeast Saccharomyces cerevisiae and ... Reactive oxygen species levels increase with age in these mutant strains and show a similar pattern to the pattern of DNA ... In wild-type S. cerevisiae, DNA damage rates increased 3-fold with age, but more than 5-fold in mutants deleted for either the ... In the fission yeast Schizosaccharomyces pombe, deficiency of mitochondrial superoxide dismutase SOD2 accelerates chronological ...
The flip recombinase (or FLP) is a gene from the commonly studied yeast Saccharomyces cerevisiae which recognizes "flip ... Genetic mosaics are a particularly powerful tool when used in the commonly studied fruit fly, where specially-selected strains ...
"Dissection of the assembly pathway of the proteasome lid in Saccharomyces cerevisiae". Biochemical and Biophysical Research ... doi:10.1016/j.str.2006.05.019. PMID 16843899.. *^ Krüger E, Kloetzel PM, Enenkel C (2001). "20S proteasome biogenesis". ... The Yeast 26S Proteasome with list of subunits and pictures. *. Ciechanover A (September 2005). "Early work on the ubiquitin ... Yet another type of non-ATPase regulatory particle is the Blm10 (yeast) or PA200/PSME4 (human). It opens only one α subunit in ...
"Identification of novel filament-forming proteins in Saccharomyces cerevisiae and Drosophila melanogaster". 》The Journal of ... doi:10.1016/j.str.2004.02.005. PMID 15016359.. *↑ Froguel P, Zouali H, Vionnet N, Velho G, Vaxillaire M, Sun F, Lesage S, ... "라는 의미로 영어로 "in yeast" 라는 뜻)에서 유래한 "효소(enzyme)"라는 용어를 만들었다.[12] 한자 번역어인 효소(酵素)는 "효모(酵母, yeast)에 들어있는 어떤 요소(要素)"라는 뜻이다. 효소라는 단어는 ... doi:10.1016/j.str.2008.10.001. PMID 19000810.. *↑ Petsko GA, Ringe D (2003). 》Chapter 1: From sequence to structure》. 》Protein ...
... mutants using Saccharomyces cerevisiae". FEMS Yeast Research. 9 (8): 1226-35. doi:10.1111/j.1567-1364.2009.00581.x. PMID ... "WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta strain in the presence of WIP". Biochemical and ...
Kaeberlein M.; McVey M.; Guarente L. (1999). "The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae ... Piper, Peter W. (2006). "Long-lived yeast as a model for ageing research". Yeast. 23 (3): 215-26. doi:10.1002/yea.1354. PMID ... Therefore, while some strains respond to calorie restriction with increased lifespan, in others calorie restriction shortens it ... Many studies were undertaken on budding yeast and fission yeast to analyze the cellular mechanisms behind increased longevity ...
Saccharomyces cerevisiae: the yeasts in the culture convert some of the sugars to ethanol which can undergo secondary reactions ... A. oryzae: Strains with high proteolytic capacity are used for brewing soy sauce.[29] ... Pasteurization: The raw soy sauce is heated to eliminate any active yeasts and molds remaining in the soy sauce and can be ... Lactic acid bacteria ferments the sugars into lactic acid and yeast makes ethanol, which through aging and secondary ...
First, the species is compatible with the main wine yeast Saccharomyces cerevisiae, though in cases where both MLF and ... with the rate of citric use/diacetyl formation influenced by the particular bacterial strain (with most strains of O. oeni ... Second, most strains of O. oeni are tolerant to the low pH levels of wine and can usually deal with the standard alcohol levels ... Several microbes can be a source for VA, including Acetobacter, Brettanomyces, and film yeast such as Candida, as well as LAB. ...
... the complete DNA sequence of the yeast Saccharomyces cerevisiae chromosome II.[29] Leroy E. Hood's laboratory at the California ... and Maclyn McCarty demonstrating that purified DNA could change one strain of bacteria into another. This was the first time ... and Saccharomyces cerevisiae at a cost of US$0.75 per base. Meanwhile, sequencing of human cDNA sequences called expressed ... "Origins of the Strain Causing an Outbreak of Hemolytic-Uremic Syndrome in Germany". N Engl J Med. 365 (8): 709-17. doi:10.1056 ...
"Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A". The ... doi:10.1016/j.str.2004.07.010. الوسيط ,التاريخ=. تم تجاهله (مساعدة); الوسيط ,الصفحات=. تم تجاهله (مساعدة); الوسيط ,العنوان=. تم ... "Characterization of mammalian eIF2A and identification of the yeast homolog". The Journal of Biological Chemistry. 277 (40): ...
Saccharomyces cerevisiae -. Malassezia (commensal yeast) Antigen mixtures. Aspergillus fumigatus, Candida albicans hsp60, as ... doi:10.1016/j.str.2011.02.004. PMC 3075535. PMID 21481769.. *^ Zhu J, Krishnegowda G, Li G, Gowda DC (July 2011). " ... and yeast via stimulation of NF-κB.[7] ...
... most notably the model organism Saccharomyces cerevisiae.[72] The presence of RNAi in other budding yeast species such as ... elegans strains indicating that A→I RNA editing may counteract RNAi silencing of endogenous genes and transgenes.[66] ... In fission yeast this complex contains argonaute, a chromodomain protein Chp1, and a protein called Tas3 of unknown function.[ ... Most studies have focused on the mating-type region in fission yeast, which may not be representative of activities in other ...
str. 465.-484., 498.-501.. ISBN 978-0-7167-4684-3. *↑ C. Usher, J. Remington, P. Martin, G. Drueckhammer, A very short hydrogen ... Struktura fumaraze kvasca Saccharomyces cerevisiae[10]. Reakcija 7: FumarazaUredi. ΔG'°=-3.8 kJ/mol ... Crystal structures of native and recombinant yeast fumarase. J.Mol.Biol. v 280 431.-442., 1998. ... Voet, D.; Voet, J. G. (2004). Biochemistry (3rd izd.). New York: John Wiley & Sons, Inc.. str. 615. ...
MacDiarmid, C.W.; Gardner, R. C. (1998). "Overexpression of the Saccharomyces cerevisiae magnesium transport system confers ... The transport of Mg2+ into mitochondria probably uses ΔΨ as in the mitochondria of yeast, and it is likely that chloroplasts ... In magnesium transport knockout strains of bacteria, healthy rates are maintained only with exposure to very high external ... In single-cell organisms such as bacteria and yeast, low levels of magnesium manifests in greatly reduced growth rates. ...
The most commonly present yeast species in the production of naturally leavened dough is Saccharomyces exiguus, which is more ... acid-tolerant than commercially produced S. cerevisiae, although the latter and other strains may also be present. Research has ... Sourdough is thus a stable culture of lactic acid bacteria and yeast in a mixture of flour and water. The yeast produces carbon ... The bacteria metabolizes sugars that the yeast cannot, and the yeast metabolizes byproducts of bacterial fermentation. ...
By the late 18th century, two yeast strains used in brewing had been identified: Saccharomyces cerevisiae (top-fermenting yeast ... An example of a top-cropping yeast is Saccharomyces cerevisiae, sometimes called an "ale yeast".[52] Bottom-cropping yeasts are ... cerevisiae, though not all strains of the species are suitable.[62] Different S. cerevisiae yeast strains have differing ... The term "yeast" is often taken as a synonym for Saccharomyces cerevisiae,[11] but the phylogenetic diversity of yeasts is ...
Herskowitz I (1988). "Life cycle of the budding yeast Saccharomyces cerevisiae". MICROBIOLOGICAL REVIEWS ... 12,0 12,1 Http://wiki.yeastgenome.org/index.php/Commonly used strains ... S. cerevisiae. Meyen ex E.C. Hansen, 1883[2] Pagaripärm ehk leiva-pärmkottseen (Saccharomyces cerevisiae)[2][3] on kottseente ... 2012)."Role of social wasps in Saccharomyces cerevisiae ecology and evolution." *↑ 5,0 5,1 5,2 Botstein D, Chervitz SA, Cherry ...
The yeast Saccharomyces cerevisiae and Saccharomyces paradoxus have a life cycle that alternates between long periods of ... A new phenomenon revealed from a genetic study of 43 strains ofSaccharomyces cerevisiae derived from natural fermentation of ... "Population genomics of the wild yeast Saccharomyces paradoxus: Quantifying the life cycle". Proceedings of the National Academy ... grape musts". Yeast. 10 (12): 1543-1552. doi:10.1002/yea.320101203. PMID 7725789.. ...
... designed to detect protein-protein interactions using the Gal4 transcriptional activator of the yeast Saccharomyces cerevisiae ... Yeast strains R2HMet and BK100 have also been used.[20] Candida albicans[edit]. C. albicans is a yeast with a particular ... S. cerevisiae[edit]. The yeast S. cerevisiae was the model organism used during the two-hybrid technique's inception. It is ... This mutant yeast strain can be made to incorporate foreign DNA in the form of plasmids. In yeast two-hybrid screening, ...
Yeast (Saccharomyces cerevisiae) has long been an important model organism for the eukaryotic cell, while the fruit fly ... These are six Prochlorococcus strains, seven marine Synechococcus strains, Trichodesmium erythraeum IMS101 and Crocosphaera ... chromosome III of brewer's yeast Saccharomyces cerevisiae (315 kb) was sequenced.[26] The first free-living organism to be ... cerevisiae (12.1 Mb), and since then genomes have continued being sequenced at an exponentially growing pace.[28] As of October ...
... the yeast component generally includes Saccharomyces cerevisiae, along with other species; the bacterial component almost ... The remaining kombucha is strained and bottled for a secondary ferment for a few days or stored at a temperature of 4℃.[34] ... and yeasts (as of the genera Brettanomyces and Saccharomyces) grown to produce a fermented beverage held to confer health ... Kombucha is produced by fermenting sugared tea using a symbiotic culture of bacteria and yeast (SCOBY) commonly called a " ...
The fungi have several unicellular species, such as baker's yeast (Saccharomyces cerevisiae) and fission yeast ( ... Strain 121, a hyperthermophilic archaea, has been shown to reproduce at 121 °C (250 °F), and survive at 130 °C (266 °F).[1] ... The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe are important model organisms in science, since they are ... yeast is used to convert sugar, grape juice, or malt-treated grain into alcohol. other microorganisms may also be used; a mold ...
Thr-rich domains are associated with genetic variation and morphogenesis in Saccharomyces cerevisiae". Yeast. 23 (8): 633-640. ... a) Replication of the STR locus has proceeded without a mutation. (b) Replication of the STR locus has led to a gain of one ... "FBI CODIS Core STR Loci". Retrieved 2010-09-20.. *^ Butler J.M. (2005). Forensic DNA Typing: Biology, Technology, and Genetics ... Forensic STR profiles are stored in DNA databanks such as the UK National DNA Database (NDNAD), the American CODIS or the ...
Za pripravo piva se najpogosteje uporabljajo kvasovke Saccharomyces cerevisiae (za varjenje ale piva) ter Saccharomyces uvarum ... Boekhout T. & Robert V. (2003). Yeasts in Food: Beneficial and Detrimental Aspects str. 370-371, Behr's Verlag, ISBN 3-86022- ... Ostergaard S., Olsson L., Nielsen J. (2000). »Metabolic Engineering of Saccharomyces cerevisiae«. Microbiol. Mol. Biol. Rev. 64 ... str. 54-57. ISBN 0-8122-3795-1. .. (Google Books) *↑ Nelson M. (2005). The Barbarian's Beverage: A History of Beer in Ancient ...
Glycosidic bond Mannan Mannose Saccharomyces cerevisiae extracts PGG-glucan, EpiCor, nutritional yeast Oyofo, BA; Deloach, JR; ... In controlled studies with chickens, a reduction in the prevalence and concentration of different strains of Salmonella, as ... derive from the cell wall of the yeast, Saccharomyces cerevisiae. The initial interest in using MOS to protect gastrointestinal ... The form present in the cell wall of Saccharomyces cerevisiae (α-1,3 and α-1,6 branched mannans; for more details see Structure ...
In Yeast genomes, (Saccharomyces cerevisiae) there are five distinct retrotransposon families: Ty1, Ty2, Ty3, Ty4 and Ty5.[29] ... multi-antibiotic resistant bacterial strains can be generated in this way). Bacterial transposons of this type belong to the Tn ... One study estimated the rate of transposition of a particular retrotransposon, the Ty1 element in Saccharomyces cerevisiae. ... a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence". Genome Research ...
Taxonomy - Saccharomyces cerevisiae (strain VIN 13) (Bakers yeast) Basket 0. (max 400 entries)x ...
Seventeen Saccharomyces cerevisiae strains, including commercial wine yeast strains, were evaluated in laboratory-scale wine ... Discrepancy in glucose and fructose utilisation during fermentation by Saccharomyces cerevisiae wine yeast strains. Authors. * ... We ranked the S. cerevisiae strains according to their rate of increase in GF discrepancy and we showed that this rate of ... utilisation during fermentation is not a fixed parameter but is dependent on the inherent properties of the yeast strain and on ...
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.. R S ... A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.. R S ... A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.. R S ... A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. ...
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.. ... yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae ... Transplacement vectors were constructed and used to derive yeast strains containing nonreverting his3, trp1, leu2 and ura3 ... These pRS vectors are all uniform in structure and differ only in the yeast selectable marker gene used (HIS3, TRP1, LEU2 and ...
Transplacement vectors were constructed and used to derive yeast strains containing nonreverting his3, trp1, leu2 and ura3 ... A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in ... A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae ... yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae ...
Interaction was followed in mixed fermentations with 1:1 inoculation of S. cerevisiae and ten non-Saccharomyces strains. ... Interaction was followed in mixed fermentations with 1:1 inoculation of S. cerevisiae and ten non-Saccharomyces strains. ... cerevisiae played the main role in the decreased culturability of the other non-Saccharomyces yeasts. However, changes in the ... cerevisiae played the main role in the decreased culturability of the other non-Saccharomyces yeasts. However, changes in the ...
FEMS Yeast Research" on DeepDyve, the largest online rental service for scholarly research with thousands of academic ... "Phenotypic evaluation and characterization of 21 industrial Saccharomyces cerevisiae yeast strains, ... Yeast, Saccharomyces cerevisiae, industrial yeast, laboratory yeast, hydrolysate, biotechnology INTRODUCTION Yeasts have served ... Yeast, Saccharomyces cerevisiae, industrial yeast, laboratory yeast, hydrolysate, biotechnology INTRODUCTION Yeasts have served ...
Chemical and volatile composition of lychee wines fermented with four commercial Saccharomyces cerevisiae yeast strains. ... Chemical and volatile composition of lychee wines fermented with four commercial Saccharomyces cerevisiae yeast strains. ... the chemical and volatile composition of lychee wines fermented with four commercial yeast strains of Saccharomyces cerevisiae ... Yeast cell population, pH, malic acid, ammonia and some amino acids had significant differences between strain 71B and other ...
We observed that some cryotolerant Saccharomyces yeasts, particularly S. uvarum, seriously compromised S. cerevisiae fitness ... We observed that some cryotolerant Saccharomyces yeasts, particularly S. uvarum, seriously compromised S. cerevisiae fitness ... In the present study we analyzed the survival capacity of non-cerevisiae strains in competition with S. cerevisiae during ... In the present study we analyzed the survival capacity of non-cerevisiae strains in competition with S. cerevisiae during ...
Saccharomyces cerevisiae strain:Bread strain, Wine strain, Bioethanol strain (bakers yeast). Saccharomyces cerevisiae strain: ... Saccharomyces cerevisiae strain:Bread strain, Wine strain, Bioethanol strain Saccharomyces cerevisiae strain:Bread strain, Wine ... strain, Bioethanol strain. Saccharomyces cerevisiae strain:Bread strain, Wine strain, Bioethanol strain Transcriptome or Gene ... Bread strain, Wine strain, Bioethanol strain Transcriptome or Gene expression. See Genome Information for Saccharomyces ...
Current genome assemblies are based on short-read sequencing data scaffolded based on homology to strain S288C. ... The haploid Saccharomyces cerevisiae strain CEN.PK113-7D is a popular model system for metabolic engineering and systems ... Date: 13th September 2017 | Source: FEMS Yeast Research. Authors: Alex N. Salazar, Arthur R. Gorter de Vries, Marcel van ... The haploid Saccharomyces cerevisiae strain CEN.PK113-7D is a popular model system for metabolic engineering and systems ...
Yeast Strains and Media. The S. cerevisiae strains used in these experiments are derivatives of S288C and are listed in Table I ... 1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae ... The Saccharomyces cerevisiae cell cycle. In The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance. J.N. ... 1994) New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10:1793-1808, ...
Saccharomyces cerevisiae (strain CEN.PK113-7D) (Bakers yeast)Imported. Automatic assertion inferred from database entriesi ... tr,N1P4W8,N1P4W8_YEASC Cak1p OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_3354 PE=4 SV=1 ...
Yeast strains and culture conditions. All yeast strains were grown at 30° in YPD broth while shaking at 225 rpm (Table 1). ... yeast. The Saccharomyces cerevisiae myosin type II (Myo1p) is found in the contractile ring that contributes to its function in ... Novel Interactome of Saccharomyces cerevisiae Myosin Type II Identified by a Modified Integrated Membrane Yeast Two-Hybrid ( ... Nonmuscle myosin type II (Myo1p) is required for cytokinesis in the budding yeast Saccharomyces cerevisiae. Loss of Myo1p ...
The genome sequences of more than 100 strains of the yeast Saccharomyces cerevisiae have been published. Unfortunately, most of ... abstractNote = {The genome sequences of more than 100 strains of the yeast Saccharomyces cerevisiae have been published. ... Title: Genome sequence and analysis of a stress-tolerant, wild-derived strain of Saccharomyces cerevisiae used in biofuels ... Accepted Manuscript: Genome sequence and analysis of a stress-tolerant, wild-derived strain of Saccharomyces cerevisiae used in ...
sp,P46680,AIP1_YEAST Actin-interacting protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AIP1 PE= ... Yeast chromosome XIII. Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names ... Yeast. Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD ... Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast). ,p>This subsection of the ,a href="http://www.uniprot. ...
... strains, the open reading frame (ORF) deletion strains of the ,em>Saccharomyces cerevisiae,/em> Genome Deletion Project (SGDP ... The collection offers over 32,000 yeast genetic strains, including the historic Yeast Genetic Stock Center (YGSC) ... and a collection of ,em>Cryptococcus neoformans ,/em>ORF deletion strains. ... ATCC is a trusted resource center housing a diverse assortment of filamentous fungi and yeasts, representing over 7,600 species ...
Strain Information. *Information. Single strain isolate from a single-strain-based traditional farmhouse kveik brewing yeast ... Saccharomyces cerevisiae Details. Saccharomyces cerevisiae A licence fee may be applied to products purchased for commercial ... Single strain isolate from a single-strain-based traditional farmhouse kveik brewing yeast culture from the Stranda valley, ... HabitatSingle strain isolate from a single-strain-based traditional farmhouse kveik brewing yeast culture from the Stranda ...
Strain Information. *Information. Respiratory petite colony mutant derived from ale yeast NCYC 239 treated with 50ppm ... Respiratory deficient mutant derived from ale yeast NCYC 239 Saccharomyces cerevisiae Details. Saccharomyces cerevisiae ... Saccharomyces cerevisiae. Pre 2011 Name. Saccharomyces cerevisiae Depositor. J.W. Millibank Deposit Date. Unknown 1963 Habitat ... National Collection of Yeast Cultures, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK ...
Saccharomyces cerevisiae, is not known to be oleaginous. However, an industrial wild-type strain, D5A, was shown to accumulate ... as well as the transcriptional profiles of the oleaginous industrial strain, D5A, and a non-oleaginous laboratory strain, ... To elucidate the mechanisms of S. cerevisiae D5A oleaginicity, we compared physiological and metabolic changes; ... cerevisiae D5A. Rather than TAG assembly from acyl groups, the major switches for the enhanced lipid accumulation of D5A (i.e ...
Yeast strain. Relevant genotype. Glycerol-3-phosphate dehydrogenase (μmol mg protein−1 min−1). Acetaldehyde dehydrogenase ( ... Elimination of Glycerol Production in Anaerobic Cultures of a Saccharomyces cerevisiae Strain Engineered To Use Acetic Acid as ... Elimination of Glycerol Production in Anaerobic Cultures of a Saccharomyces cerevisiae Strain Engineered To Use Acetic Acid as ... Elimination of Glycerol Production in Anaerobic Cultures of a Saccharomyces cerevisiae Strain Engineered To Use Acetic Acid as ...
Rapid asymmetric evolution of Saccharomyces cerevisiae wine yeasts under apparently non-selective conditions. Yeast22:1299-1306 ... Yeast strains, culture media, and phenotype tests.SMR10-11D (MATα/MATaHO/HO SMRR/SMRR [k2+]) is a killer wine yeast (1). SMR10- ... Most wild strains of Saccharomyces cerevisiae carry recessive deleterious alleles in the heterozygous state (4, 5, 7, 10, 13), ... Analysis of Homothallic Saccharomyces cerevisiae Strain Mating during Must Fermentation Message Subject (Your Name) has ...
Yeast strains and media. Unless otherwise noted, standard media and genetic methods were used (Rose and Fink, 1990). 1% ... In response to starvation, cells of the yeast Saccharomyces cerevisiae undergo meiosis and form spores (Byers, 1981). In this ... S. cerevisiae strains used in this study are listed in supplementary material Table S1. HI26 (erv14Δ/erv14Δ), HI29 (vps13Δ/ ... Sporulation of Saccharomyces cerevisiae is a developmental process in which four haploid spores are created within a single ...
Saccharomyces cerevisiae strains. Screening a Genomic Library.. A YCp50-based yeast genomic library (30) was obtained from M. ... Yeast Strains and Cell Culture.. The yeast strains used in this study are listed in Table 1. WX17-4a was isolated from MD-2/ ... M. Rose for the yeast genomic library, Drs. S. Bacchetti and L. Ma for a yeast mms2Δ strain, Dr. V. Chau for sharing ... The RAD6 gene in Saccharomyces cerevisiae encodes a 172-amino acid, 20-kDa E2 enzyme, Ubc2 (4, 8). Rad6 appears to be a ...
Saccharomyces cerevisiae, synthetic promoter, inhibitors, NADPH, library. in Yeast. volume. 20. issue. 15. pages. 1263 - 1272. ... activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains.. ... to reduce the xylitol yield and the xylose consumption in the xylose-utilizing recombinant Saccharomyces cerevisiae strain ... to reduce the xylitol yield and the xylose consumption in the xylose-utilizing recombinant Saccharomyces cerevisiae strain ...
... cerevisiae,/italic, strains. I analyze genome sequence, gene expression, protein abundance, metabolite abundance, and cellular ... Patterns and determinants of variation in functional genomics phenotypes in the yeast Saccharomyces cerevisiae. ... Saccharomyces cerevisiae,/italic, to make inferences about the relationship between DNA sequences and the molecular phenotypes ... In this dissertation, I outline computational methods I have developed and analyses I have conducted in the yeast ,italic, ...
There are two major factors conferring multidrug resistance in S. cerevisiae: one is the drug efflux system and the other is ... To use yeast for chemical biology research, however, it has been necessary to construct yeast strains suitable for various ... In this review, we discuss the construction of our multidrug-sensitive yeast strains and their application in chemical biology. ... We therefore constructed a strain which shows high sensitivity to multiple drugs by disrupting the drug efflux system using ATP ...
We refer to the resulting accumulation profile as the yeast ionome. We identified 212 strains that showed altered ionome ... Surprisingly few of these mutants (four strains) were affected for only one element. Rather, levels of multiple elements were ... Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation ... We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. ...
Nygård Y. Characterization of D-xylonate producing Kluyveromyces lactis and Saccharomyces cerevisiae yeast strains: Masters ... Nygård, Y. (2010). Characterization of D-xylonate producing Kluyveromyces lactis and Saccharomyces cerevisiae yeast strains: ... Characterization of D-xylonate producing Kluyveromyces lactis and Saccharomyces cerevisiae yeast strains : Masters thesis. / ... Characterization of D-xylonate producing Kluyveromyces lactis and Saccharomyces cerevisiae yeast strains : Masters thesis. ...
  • Any fungal metabolite produced during a metabolic reaction in Baker's yeast ( Saccharomyces cerevisiae ). (ebi.ac.uk)
  • In 1996 Saccharomyces cerevisiae or baker's yeast was the first eukaryotic organism to have its entire nuclear genome sequenced. (brighthub.com)
  • But Saccharomyces boulardii is different from other strains of Saccharomyces cerevisiae commonly known as brewer's yeast and baker's yeast. (medlineplus.gov)
  • The version of ADH found in yeast, specifically baker's yeast, is different than the ADH found naturally in the human body. (seriouseats.com)
  • But now it is believed to be a strain of Saccharomyces cerevisiae (baker's yeast). (webmd.com)
  • Here, we investigate the fermentation kinetics of glucose and fructose and the influence of certain environmental parameters on hexose utilisation by wine yeast. (wiley.com)
  • The discrepancy between glucose and fructose utilisation increased during the course of fermentation in a strain-dependent manner. (wiley.com)
  • We ranked the S. cerevisiae strains according to their rate of increase in GF discrepancy and we showed that this rate of increase is not correlated with the fermentation capacity of the strains. (wiley.com)
  • These results show that the discrepancy between glucose and fructose utilisation during fermentation is not a fixed parameter but is dependent on the inherent properties of the yeast strain and on the external conditions. (wiley.com)
  • The present study analyzes the lack of culturability of different non- Saccharomyces strains due to interaction with Saccharomyces cerevisiae during alcoholic fermentation. (frontiersin.org)
  • Strain differences in culturability and nutrient consumption (glucose, alanine, ammonium, arginine, or glutamine) were found within each species in mixed fermentation with S. cerevisiae . (frontiersin.org)
  • Cell-free S. cerevisiae supernatants induced faster culturability loss than synthetic media corresponding to the same fermentation stage. (frontiersin.org)
  • Culturability differences were observed among species and strains in culture assays and thus showed distinct tolerance to S. cerevisiae metabolites and fermentation environment. (frontiersin.org)
  • Spontaneous wine fermentation is driven by a succession of different yeast species. (frontiersin.org)
  • A great variety of non- Saccharomyces yeast species originate from grape berries and survive during the early stages of fermentation, such as species from the genera Candida, Hanseniaspora, Lachancea, Metschnikowia, Pichia , and Torulaspora ( Fleet, 2003 ). (frontiersin.org)
  • However, fermentative Saccharomyces cerevisiae soon replaces non- Saccharomyces species to become the main or the only species present in the late stages of fermentation. (frontiersin.org)
  • These industrial strains are isolated from industrial fermentation sites, and they are considered as potential host strains for superior fermentation processes. (deepdyve.com)
  • In many cases, industrial yeast strains have higher thermotolerance, increased resistances towards fermentation inhibitors and increased glucose fermentation rates under anaerobic conditions when compared with laboratory yeast strains. (deepdyve.com)
  • Through screening and phenotypic characterization of commercially available industrial yeast strains, industrial fermentation processes requiring specific environmental conditions may be able to select an ideal starting yeast strain to be further engineered. (deepdyve.com)
  • Here, we have characterized and compared 21 industrial S. cerevisiae strains under multiple conditions, including their tolerance to varying pH conditions, resistance to fermentation inhibitors, sporulation efficiency and ability to ferment lignocellulosic sugars. (deepdyve.com)
  • Although the production of baked goods and fermented foods or beverages has historically been the major industrial application for S. cerevisiae, new avenues of yeast fermentation capabilities were discovered in recent decades. (deepdyve.com)
  • Industrial strains of S. cerevisiae are known to have higher tolerances against harsh industrial environments, such as lower pH, fermentation inhibitors, osmolality and higher temperature. (deepdyve.com)
  • The majority of bioethanol produced by S. cerevisiae is from the fermentation of sugarcane or corn-derived glucose (Wheals et al.1999: 482-7). (deepdyve.com)
  • Although industrial yeast fermentation has resulted in the annual production of more than 50 billion liters ethanol production in the United States alone, the availability of corn and sugarcane is a limiting step in using biofuels as a total replacement for fossil fuels (Chum et al.2014: 205-23). (deepdyve.com)
  • Saccharomyces cerevisiae is the main microorganism responsible for the fermentation of wine. (frontiersin.org)
  • However, S . cerevisiae can competitively displace other yeast species from wine fermentations, therefore the use of these new starters requires an analysis of their behavior during competition with S. cerevisiae during wine fermentation. (frontiersin.org)
  • In the present study we analyzed the survival capacity of non- cerevisiae strains in competition with S. cerevisiae during fermentation of synthetic wine must at different temperatures. (frontiersin.org)
  • From an enological point of view, mixed co-cultures between S. cerevisiae and S. paradoxus or S. eubayanus , deteriorated fermentation parameters and the final product composition compared to single S. cerevisiae inoculation. (frontiersin.org)
  • However, in co-inoculated synthetic must in which S. kudriavzevii or S. uvarum coexisted with S. cerevisiae , there were fermentation performance improvements and the final wines contained less ethanol and higher amounts of glycerol. (frontiersin.org)
  • Wine is the product of complex interactions among yeast, bacteria and other fungi that begin in vineyards and continue with the fermentation process. (frontiersin.org)
  • However, other Saccharomyces species ( Saccharomyces non- cerevisiae yeasts, SNC) may play an important role in wine fermentation under certain conditions. (frontiersin.org)
  • By contrast, our knowledge about yeast physiology during solid state processes, such as bread dough, cheese or cocoa fermentation remains limited. (nih.gov)
  • We investigated changes in the transcriptome of three genetically distinct Saccharomyces cerevisiae strains during bread dough fermentation. (nih.gov)
  • Dynamics of the Saccharomyces cerevisiae transcriptome during bread dough fermentation. (nih.gov)
  • Flor strains of Saccharomyces cerevisiae form a biofilm on the surface of wine at the end of fermentation, when sugar is depleted and growth on ethanol becomes dependent on oxygen. (uniss.it)
  • LOH was not detected in genetically stable wine yeasts during must fermentation. (asm.org)
  • However, after sporulation, the heterozygosity of the new yeast population decreased during must fermentation. (asm.org)
  • This causes loss of heterozygosity (LOH) and should eliminate the recessive lethal or deleterious alleles that decrease yeast fitness leading to slower growth, lower fermentation rate, reduced spore viability, etc. (asm.org)
  • This strategy, which probably occurs in nature, has been applied in the laboratory to obtain new fitness-improved wine yeasts which are more suitable for industrial fermentation ( 13 ). (asm.org)
  • Also, a high LOH has been reported at the URA3 locus in a transgenic wine yeast strain during must fermentation ( 12 ). (asm.org)
  • In the study, we used new wine yeast strains with good fermentation performance, high rates of sporulation and spore viability, and appropriate genetic markers to analyze the frequency of mating between the different yeasts living in the same fermenting must. (asm.org)
  • The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains. (lu.se)
  • By accumulating endogenous lycopene during the aerobic fermentation of the seed stage, the intracellular ROS level of strain decreased to 1.4% of that of the control strain during ethanol fermentation. (springer.com)
  • In the ethanol fermentation system containing 100 g/L glucose and 5.5 g/L acetic acid, the lag phase of strain was 24 h shorter than that of control strain. (springer.com)
  • The flavor of makgeolli is primarily determined by metabolic products such as free sugars, amino acids, organic acids, and aromatic compounds, which are produced during the fermentation of raw materials by molds and yeasts present in nuruk, a Korean fermentation starter. (bvsalud.org)
  • In this study, makgeolli was brewed using the wild yeast strain Saccharomyces cerevisiae Y98-5, and temporal changes in the metabolites during fermentation were analyzed by ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. (bvsalud.org)
  • Yeast proteome variations reveal different adaptive responses to grape must fermentation. (semanticscholar.org)
  • We finally decided to use ale yeast because we don't have the facilities to control the cooler fermentation required for a lager beer . (sunset.com)
  • I have tried this style and found it to be much faster to start fermentation and less expensive then liquid yeast but you won't find the varieties of different strains that you find in liquid yeast. (sunset.com)
  • To give you an idea of the many different strains of yeast you can use for fermentation, Williams catalog has 15 types of liquid ale yeast alone. (sunset.com)
  • The interesting part is that they all start with a basic wheat beer base and are deviated by the different strains of yeast introducedinto the wort for fermentation. (sunset.com)
  • By fermentation , the yeast species Saccharomyces cerevisiae converts carbohydrates to carbon dioxide and alcohols - for thousands of years the carbon dioxide has been used in baking and the alcohol in alcoholic beverages. (wikipedia.org)
  • In 1857, French microbiologist Louis Pasteur proved in the paper " Mémoire sur la fermentation alcoolique " that alcoholic fermentation was conducted by living yeasts and not by a chemical catalyst. (wikipedia.org)
  • Almost 150 years ago, Pasteur showed that fermentation is a biological process conducted by microorganisms including Saccharomyces cerevisiae, and subsequently, S. cerevisiae's physiology, genetics and molecular biology have been scrutinized. (scribd.com)
  • The USDA developed a new yeast that naturally produces beta-glucosidase and ethanol so no beta-glucosidase needs to be added to complete a one-step simultaneous saccharification and fermentation (SSF) for cellulosic ethanol production. (usda.gov)
  • This powdery strain results in yeast that remain in suspension post fermentation. (wyeastlab.com)
  • 9) Grapes are usually fermented by wild yeasts but fermentation can be induced by very high CO2. (bio.net)
  • I have read a few folks post that they liked their beers fermented at 68 °F with T-58 and since that temperature is within the recommended ranges of these two yeast strains I decided to use the temperature of 68 °F for the first 3 days (72 hours) of the fermentation. (beeradvocate.com)
  • I am guessing that most of the work being done by the T-58 strain will be completed at this point in time so I chose to then let the fermentation temperature free rise to permit the BE-134 to complete the job. (beeradvocate.com)
  • ATCC is a trusted resource center housing a diverse assortment of filamentous fungi and yeasts, representing over 7,600 species. (atcc.org)
  • The ATCC Mycology Collection has approximately 330 species of biomedical fungi and yeasts, with a total exceeding 2000 isolates. (atcc.org)
  • ATCC provides all the cultures you need to perform complex analysis of fungal and yeast sequenced genomes. (atcc.org)
  • ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains. (atcc.org)
  • ATCC's Preceptrol cultures offer an economical way for your lab to use well-characterized ATCC strains. (atcc.org)
  • ATCC offers fully characterized strains for quality control testing, including strains that have been recommended as controls for rapid identification, tests on food and water, and use with commercial kits and instrumentation. (atcc.org)
  • ATCC houses an assortment of Saccharomyces cerevisiae deletion mutants to support genetic research. (atcc.org)
  • The type strain of T. delbrueckii is CBS 1146T, equivalent to CLIB 230 or ATCC 10662, etc. (wikipedia.org)
  • Our annotation predicts 92 genes that are not present in the reference genome of the laboratory strain S288c, over 70% of which were expressed. (osti.gov)
  • We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae . (biomedcentral.com)
  • This effort resulted in a collection of mutant strains disrupted in most of the approximately 6,000 genes in the yeast genome. (biomedcentral.com)
  • In this study, an automated two-hybrid interaction protocol was used to find yeast genes encoding proteins that bind XI to identify potential targets for improving xylose utilization by S. cerevisiae. (elsevier.com)
  • Binding of a yeast ORF protein to XI activates two chromosomally located reporter genes in a PJ69-4 yeast strain to give selective growth. (elsevier.com)
  • Many of the genes that are involved in the control of the cell cycle are conserved between humans and yeast. (brighthub.com)
  • It is often the genes that are involved in cell proliferation that are mutated in cancer cells and so yeast can be used to study anticancer drugs to see if they are hitting, and neutralising their molecular targets. (brighthub.com)
  • In February 2009 scientists there discovered a prion - an infectious mis-folded protein - inside brewer's yeast that affects the expression of yeast genes (as does the normal version of the protein). (brighthub.com)
  • Saccharomyces cerevisiae transcribes two genes, ARE1 and ARE2 , that contribute disproportionately to the esterification of sterols. (asm.org)
  • Fragments of the ADP1, ACC1, RPN2, GLN4, and ALA1 genes were amplified by PCR from chromosomal DNA of 18 wine Saccharomyces cerevisiae strains. (semanticscholar.org)
  • Sensing of extracellular amino acids results in transcriptional induction of amino acid permease genes in yeast. (asm.org)
  • Because several genes in S. cerevisiae encode permeases capable of transporting branched-chain amino acids, the screen revealed mutations that reduced the expression of all of them simultaneously, thereby focusing on components of the signaling pathway itself. (asm.org)
  • A hap1 mutation in a laboratory strain of Saccharomyces cerevisiae results in decreased expression of ergosterol-related genes and cellular ergosterol content compared to sake yeast. (semanticscholar.org)
  • The characterization of the HPG mutants and identification of relevant genes allow us to unravel the complex regulatory mechanism of the yeast tryptophan permeases Tat1 and Tat2 with respect to ubiquitination, deubiquitination, and endocytic trafficking in the cell. (springer.com)
  • Abe F, Minegishi H (2008) Global screening of genes essential for growth in high-pressure and cold environments: searching for basic adaptive strategies using a yeast deletion library. (springer.com)
  • Schmidt A, Hall MN, Koller A (1994) Two FK506 resistance-conferring genes in Saccharomyces cerevisiae , TAT1 and TAT2 , encode amino acid permeases mediating tyrosine and tryptophan uptake. (springer.com)
  • About 25 percent of human genes have yeast counterparts, and these human genes have frequently been shown to functionally replace the corresponding gene in the yeast cell. (encyclopedia.com)
  • As of 2000, twenty genes that determine yeast life span had been identified. (encyclopedia.com)
  • First, genes whose activity changes during the life span were isolated, followed by an examination of their causal role in yeast aging. (encyclopedia.com)
  • There are other killer systems in S. cerevisiae, such as KHR1 and KHS1 genes encoded on chromosomes IX and V, respectively. (wikipedia.org)
  • L-A virus uses yeast Ski complex (super killer) and MAK (maintenance of killer) chromosomal genes for its preservation in the cell. (wikipedia.org)
  • The first eukaryotic genome to be sequenced is that of Saccharomyces cerevisiae ( S. cerevisiae ) in 1996, and it is commonly known as brewer's yeast. (wikibooks.org)
  • The improved ability of brewer's yeasts to produce α-galactosidase provides a sensitive method for monitoring pasteurization of beer. (freepatentsonline.com)
  • I saw a dietician today and she told me brewer's yeast is gluten. (celiac.com)
  • She then sent me to buy these supplements that had , you guessed it, brewer's yeast in them. (celiac.com)
  • The employee called the manufactuer and they said they were labeled gluten-free because the brewer's yeast is sugar. (celiac.com)
  • Current methods of testing cannot accurately confirm the amount of residual gluten in brewer's yeast. (celiac.com)
  • Brewer's yeast is a kind of yeast that is a by-product of brewing beer. (webmd.com)
  • Dietary supplements containing brewer's yeast often contain non-living, dried yeast. (webmd.com)
  • People use brewer's yeast to make medicine. (webmd.com)
  • Brewer's yeast is taken by mouth for respiratory problems, including the common cold and other upper respiratory tract infections, influenza, seasonal allergies, and swine flu. (webmd.com)
  • Brewer's yeast is also taken by mouth for diarrhea, swelling of the colon (colitis) due to the bacteria Clostridium difficile, high cholesterol, loss of appetite, acne, premenstrual syndrome (PMS), recurring boils on the skin (furunculosis), type 2 diabetes and irritable bowel syndrome (IBS). (webmd.com)
  • Due to the chromium content of brewer's yeast, there is interest in using it for lowering blood glucose in people with diabetes. (webmd.com)
  • Additionally, brewer's yeast seems to increase enzymes in the intestine that could help relieve diarrhea. (webmd.com)
  • Brewer's yeast might help fight bacteria that cause infections in the intestine and improve the body's defenses against viral lung infections such as flu and the common cold. (webmd.com)
  • Brewer's yeast is a source of B vitamins and protein. (webmd.com)
  • Taking a specific brewer's yeast preparation (Sillix Donna by Giuliani) that also contains vitamins and minerals by mouth might decrease symptoms of PMS. (webmd.com)
  • Early research shows that taking 500 mg of a specific brewer's yeast product (EpiCor by Embria Health Sciences) for 12 weeks reduces nasal symptoms associated with seasonal allergies during high pollen counts. (webmd.com)
  • There is a report that taking brewer's yeast by mouth for 4 months along with vancomycin for 30 days may help treat colitis caused by the bacteria Clostridium difficile and prevent recurrence. (webmd.com)
  • Early research suggests that taking brewer's yeast containing chromium by mouth for 8 weeks can reduce blood sugar levels in people with diabetes. (webmd.com)
  • Early research suggests that taking brewer's yeast containing chromium by mouth for 8 weeks decreases blood levels of total cholesterol and increases levels of high-density lipoprotein (HDL or "good") cholesterol in people with high cholesterol. (webmd.com)
  • Some early research shows that taking a specific brewer's yeast product (EpiCor by Embria Health Sciences) for 12 weeks reduces the possibility of getting the flu in people who have not received the flu vaccine. (webmd.com)
  • However, other early research shows that taking this same brewer's yeast product reduces the risk of the common cold or flu and helps symptoms resolve faster in healthy people who recently received flu shots. (webmd.com)
  • Early research shows that taking 500 mg or 1000 mg of brewer's yeast daily for 12 weeks might reduce intestinal pain and hard stools in people with IBS with constipation. (webmd.com)
  • More evidence is needed to rate the effectiveness of brewer's yeast for these uses. (webmd.com)
  • Brewer's yeast is POSSIBLY SAFE for most people when taken by mouth short-term. (webmd.com)
  • These new opportunities are due in part to rapid and significant advances in our understanding of yeast genetics and physiology (Walker 1998: 362). (deepdyve.com)
  • We cloned the MMS2 gene from a yeast genomic library by functional complementation of the mms2-1 mutant [Prakash, L. & Prakash, S. (1977) Genetics 86, 33-55]. (pnas.org)
  • Chapter 13 in: Yeast Genetics, J. F. T. Spencer et al. (freepatentsonline.com)
  • The powerful tools of yeast genetics and cell biology have extended this description. (encyclopedia.com)
  • This has revolutionized yeast genetics. (encyclopedia.com)
  • These pRS vectors are all uniform in structure and differ only in the yeast selectable marker gene used (HIS3, TRP1, LEU2 and URA3). (genetics.org)
  • B) S. cerevisiae IMZ132 ( gpd1 Δ gpd2 Δ overexpressing the E. coli mhpF gene). (asm.org)
  • The observed LOH occurs because of the linkage of the locus MAT to the chromosome III centromere, without the necessity for self spore clone mating or the high frequency of gene conversion and rapid asymmetric LOH observed in genetically unstable yeasts. (asm.org)
  • The rad6 and rad18 mutants are defective in both pathways, and the rev3 mutant affects only the mutagenesis pathway, but a yeast gene that is involved only in error-free postreplication repair has not been reported. (pnas.org)
  • The RAD6 gene in Saccharomyces cerevisiae encodes a 172-amino acid, 20-kDa E2 enzyme, Ubc2 ( 4 , 8 ). (pnas.org)
  • Disruption of the ZWF1 gene encoding glucose-6-phosphate dehydrogenase (G6PDH) has been shown to reduce the xylitol yield and the xylose consumption in the xylose-utilizing recombinant Saccharomyces cerevisiae strain TMB3255. (lu.se)
  • This strain collection provides a unique resource for the analysis of gene function in a model eukaryotic cell. (biomedcentral.com)
  • In a screen for mutants that display synthetic lethal interaction with hpr1 Δ, a hyperrecombination mutant of Saccharomyces cerevisiae , we have isolated a novel cold-sensitive allele of the acetyl coenzyme A (CoA) carboxylase gene, acc1 cs , encoding the rate-limiting enzyme of fatty acid synthesis. (asm.org)
  • In addition, various studies of both yeast and mammalian cells have suggested that Cdc42p, through its interaction with p21-activated kinases (PAKs), plays a role in signaling pathways that regulate target gene transcription. (asm.org)
  • Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. (atcc.org)
  • To identify the yeast gene mediating the interaction of C. glabrata with epithelial cells, we undertook a mutant screen. (sciencemag.org)
  • The objects of this invention are new Saccharomyces cerevisiae yeast strains into which α-galactosidase gene (MEL + ) has been transferred by using recombinant DNA methods. (freepatentsonline.com)
  • Identification and characterization of a novel biotin biosynthesis gene in Saccharomyces cerevisiae. (semanticscholar.org)
  • Cloning and analysis of the AWA1 gene of a nonfoaming mutant of a sake yeast. (semanticscholar.org)
  • The Awa1 gene is required for the foam-forming phenotype and cell surface hydrophobicity of sake yeast. (semanticscholar.org)
  • Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BIO5, a novel gene involved in vitamer uptake. (semanticscholar.org)
  • Complete sequencing of the yeast genome and complementation of cdc25-5 have placed the CDC25 gene on chromosome XII. (bio.net)
  • FSH1 belongs to the family of serine hydrolases in yeast and is homologous to the human ovarian tumor suppressor gene ( OVAC2 ). (springer.com)
  • Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites. (sigmaaldrich.com)
  • A DNA fragment containing five copies of a chimeric human/salmon calcitonin gene (5hsCT) under the control of the promoter for phosphoglycerate kinase (P pgk ) was constructed to express 5hsCT in S. cerevisiae using ura3 as a selectable auxotrophic marker gene. (sigmaaldrich.com)
  • The budding yeast Saccharomyces cerevisiae is a useful eukaryote model organism for application to chemical biology studies, for example, drug screening, drug evaluation, and target identification. (intechopen.com)
  • The yeast Saccharomyces cerevisiae has been a useful model organism for the study of many different fundamental cellular processes, including the uptake, metabolism, and homeostatic control of mineral nutrients and trace elements. (biomedcentral.com)
  • Yeast is a unicellular organism whose DNA is packaged into chromosomes that are localized in a subcellular structure called the nucleus. (encyclopedia.com)
  • The yeast cell is at the same time the yeast organism. (encyclopedia.com)
  • Saccharomyces boulardii is called a "probiotic," a friendly organism that helps to fight off disease-causing organisms in the gut such as bacteria and yeast. (webmd.com)
  • The most important microbial production organism is the yeast Saccharomyces cerevisiae but other yeast species, as well as molds, algae, and bacteria are of potential interest for glycerol production. (scribd.com)
  • Search for Fungi and Yeast alphanumerically. (atcc.org)
  • Any eukaryotic metabolite produced during a metabolic reaction in fungi, the kingdom that includes microorganisms such as the yeasts and moulds. (ebi.ac.uk)
  • [15] Researchers were doubtful whether yeasts were algae or fungi, [16] but in 1837 Theodor Schwann recognized them as fungi. (wikipedia.org)
  • Our results show that regardless of the genetic background, all three strains exhibit similar changes in expression patterns. (nih.gov)
  • in stress tolerance and carbon metabolism and are shared with a Brazilian bioethanol production strain, even though the strains differ dramatically at most genetic loci. (osti.gov)
  • The collection offers over 32,000 yeast genetic strains, including the historic Yeast Genetic Stock Center (YGSC) strains, the open reading frame (ORF) deletion strains of the Saccharomyces cerevisiae Genome Deletion Project (SGDP), and a collection of Cryptococcus neoformans ORF deletion strains. (atcc.org)
  • Genetic instability and genome renewal may cause loss of heterozygosity (LOH) in homothallic wine yeasts ( Saccharomyces cerevisiae ), leading to the elimination of the recessive lethal or deleterious alleles that decrease yeast fitness. (asm.org)
  • In S. cerevisiae , genetic instability is associated with a high rate of LOH ( 3 , 9 , 14 ). (asm.org)
  • High genetic instability and LOH in natural wine yeasts during laboratory propagation under nonselective conditions, but not in the common laboratory strains of S. cerevisiae , have been described recently ( 3 , 14 ). (asm.org)
  • This result is mirrored by field surveys of the genetic variability of natural strains of yeast. (pnas.org)
  • Genetic inference shows that the oak S. cerevisiae are closely related to strains isolated from NZ and Australian vineyards, but that the S. paradoxus strains are very closely related to European isolates. (scribd.com)
  • The genetic distances separating the strains isolated from the same patient and those separating the strains isolated from different patients were estimated, and the results obtained with the two probes were compared. (asm.org)
  • In Strain Engineering: Methods and Protocols , powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. (springer.com)
  • Several yeast genetic databases are accessible online, which facilitates functional genome analyses. (encyclopedia.com)
  • Phenotypic evaluation and characterization of 21 industrial Saccharomyces cerevisiae yeast strains Kong, In Iok;Turner, Timothy Lee;Kim, Heejin;Kim, Soo Rin;Jin, Yong-Su 2018-02-01 00:00:00 Abstract Microorganisms have been studied and used extensively to produce value-added fuels and chemicals. (deepdyve.com)
  • Abstract Capacities of binding magnesium ions by the Saccharomyces cerevisiae bakery yeast during cultivation in stationary conditions on the YPD (control) and YPD (experimental) media enriched in 0.25. (actapol.net)
  • Current genome assemblies are based on short-read sequencing data scaffolded based on homology to strain S288C. (nanoporetech.com)
  • The project to sequence the yeast genome got underway in 1989 and the entire code of strain S288c was spelled out in 1996. (brighthub.com)
  • The haploid Saccharomyces cerevisiae strain CEN.PK113-7D is a popular model system for metabolic engineering and systems biology research. (nanoporetech.com)
  • This mating restriction slowed down the LOH process of the yeast population, maintaining the heterozygote frequency higher than would be expected assuming a fully random mating of the haploid yeasts or according to the Mortimer genome renewal proposal. (asm.org)
  • Sporulation of Saccharomyces cerevisiae is a developmental process in which four haploid spores are created within a single mother cell. (biologists.org)
  • This phenotype is also displayed by haploid cdc15 mutant strains when cell lysis is prevented by osmotic protection, and shared by other MEN mutants. (isciii.es)
  • Brewing characteristics of haploid strains isolated from sake yeast Kyokai No. 7. (semanticscholar.org)
  • Our study was based on yeast cells, whose size was determined by increased genome copy number, ranging from haploid to tetraploid. (mendeley.com)
  • Signi?cantly higher amounts of acetic acid wer e det ected in all samples fermented with strain RC 212 than in those ferme nted with strain EC1118 (0.282 and 0.602 g/l, respectively). (vtt.fi)
  • It has been reported that decreasing ROS level can improve the acetic acid tolerance of Saccharomyces cerevisiae . (springer.com)
  • In the study, we investigated effects of endogenous lycopene on cell growth and ethanol production of S. cerevisiae in acetic acid media. (springer.com)
  • Biosynthesis of endogenous lycopene is first associated with enhancing tolerance to acetic acid in S. cerevisiae . (springer.com)
  • The development of acetic acid-tolerant yeast strains is mainly through reducing the absorption of acetic acid, enhancing the efflux of hydrogen ions and acetate ions, and enhancing the intracellular metabolism of acetic acid. (springer.com)
  • Guaragnella N, Bobba A, Passarella S (2010) Yeast acetic acid induced programmed cell death can occur without cytochrome c release which requires metacaspase YCA1. (springer.com)
  • To address this question, we have combined the genomic technologies provided by the Saccharomyces Genome Deletion collection with spectroscopic methods for the simultaneous analysis of multiple mineral nutrients accumulated by cells. (biomedcentral.com)
  • The virus, L-A, is an icosahedral virus of S. cerevisiae comprising a 4.6 kb genomic segment and several satellite double-stranded RNA sequences, called M dsRNAs. (wikipedia.org)
  • Yeasts, specifically Saccharomyces cerevisiae, receive industrial attention because of their well-known ability to ferment glucose and produce ethanol. (deepdyve.com)
  • Both strains exhibited decreased xylitol yields (0.13 and 0.19 g/g xylose) and enhanced ethanol yields (0.36 and 0.34 g/g xylose) compared with the control strain TMB3001 (0.29 g xylitol/g xylose, 0.31 g ethanol/g xylose). (lu.se)
  • All fermenta- tions with immobilized yeast cells had a shorter lag phase and faster utilization of sugars and ethanol production than those fermented with suspended cells. (vtt.fi)
  • Glucose consumption rate and ethanol titer of yPS002 got to 2.08 g/L/h and 44.25 g/L, respectively, which were 2.6- and 1.3-fold of the control strain. (springer.com)
  • Commercialization of fuel ethanol production from lignocellulosic biomass has focused on engineering the glucose-fermenting industrial yeast Saccharomyces cerevisiae to use pentose sugars. (elsevier.com)
  • Baker's and distiller's yeasts producing α-galactosidase, are utilizable in the corresponding industry, because they are able to utilize the raffinose present in molasses, which results in greater yield of yeast (or ethanol) and reduction or elimination of the costs associated with biological oxygen demand (B.O.D.) in the effluent from factories. (freepatentsonline.com)
  • Yeasts have recently been used to generate electricity in microbial fuel cells , [10] and produce ethanol for the biofuel industry. (wikipedia.org)
  • Wild-type yeast cells grown on AKG were more resistant to hydrogen peroxide, menadione, and transition metal ions (Fe 2+ and Cu 2+ ) but not to ethanol and heat stress as compared with control ones. (hindawi.com)
  • The yeast themselves digest sugars into ethanol, but yeast die when too much ethanol is present, so they had to evolve a system that would allow them to keep digesting optimally without committing suicide-by-alcohol. (seriouseats.com)
  • Saccharomyces cerevisiae yeast contains the ADH enzyme that digests ethanol. (seriouseats.com)
  • 6 But the human stomach, where supposed yeast ADH metabolism of ethanol would occur, has a pH around 1.5 to 3.5 (and that's really acidic). (seriouseats.com)
  • We successfully sequenced the genome of Saccharomyces cerevisiae strain YJM789 . (stanford.edu)
  • Research shows that taking Saccharomyces boulardii by mouth helps improve the appearance of acne. (medlineplus.gov)
  • Early research shows that taking Saccharomyces boulardii does not help students do better on exams or reduce their stress. (medlineplus.gov)
  • Early research also shows that taking Saccharomyces boulardii along with mesalamine can help people with Crohn disease stay in remission longer. (medlineplus.gov)
  • Early research shows that taking Saccharomyces boulardii by mouth does not reduce yeast infections in the digestive tract of people with cystic fibrosis. (medlineplus.gov)
  • Early research shows that taking Saccharomyces boulardii might improve heart function in people with heart failure. (medlineplus.gov)
  • Research shows that taking Saccharomyces boulardii can prevent diarrhea caused by taking antibiotics. (webmd.com)
  • Early research shows that taking Saccharomyces boulardii by mouth along with antibiotics reduces diarrhea and stomach pain in people with amoeba infections. (webmd.com)
  • Sequencing the genome of strain MC58 [a serogroup B strain isolated from a case of invasive infection ( 4 )] provides an efficient means of acquiring data relevant to the detailed molecular characterization of this pathogen. (sciencemag.org)
  • An ERV14 paralog ERV15 is also present in the S. cerevisiae genome, although deletion of ERV15 has no reported phenotype and overexpression of ERV15 could not rescue the erv14 budding defect ( Powers and Barlowe, 1998 ). (biologists.org)
  • First, we used a ura3 deletion strain congenic with a virulent clinical isolate ( 4 ). (sciencemag.org)
  • Results: We have used an evolutionary engineering approach that depends on a quadruple hexokinase deletion xylose-fermenting S. cerevisiae strain to select for growth on D-xylose in the presence of high D-glucose concentrations. (rug.nl)
  • The Yeast Deletion Library and the SGTC chemogenomics platform have found applications providing new insights into the genotype-phenotye relationship on a genome-wide scale. (stanford.edu)
  • To identify novel Myo1p -interacting proteins, and determine if Myo1p can serve as a scaffold to recruit proteins to the bud neck during cytokinesis, we used the integrated split-ubiquitin membrane yeast two-hybrid (iMYTH) system. (g3journal.org)
  • Many proteins that are important to humans are studied by examining their homologs in yeasts. (wikibooks.org)
  • For example, signaling proteins and protein-processing enzymes are all discovered through the help of yeast genome. (wikibooks.org)
  • The adherence of Candida to host cells has been the subject of intense investigation, and in the case of C. albicans , the yeast expresses a number of adhesins capable of interacting with a variety of ligands, including proteins [reviewed in ( 5 )] and carbohydrates ( 5-8 ). (sciencemag.org)
  • A killer yeast is a yeast, such as Saccharomyces cerevisiae, which is able to secrete one of a number of toxic proteins which are lethal to susceptible cells. (wikipedia.org)
  • Control experiments showed that the Myo1p -bait construct was appropriately expressed, and that the protein colocalized to the yeast bud neck. (g3journal.org)
  • Here, we investigated the dynamic properties of tER sites in Saccharomyces cerevisiae and probed protein and lipid requirements for tER site structure and function. (pubmedcentralcanada.ca)
  • To gain basic insight into the protein and lipid requirements for tER site assembly, we have examined the influence of specific mutations and inhibitors on tER site structure and function in S. cerevisiae by using in vivo and cell-free approaches. (pubmedcentralcanada.ca)
  • In the budding yeast Saccharomyces cerevisiae , cells of the a and α mating types secrete pheromones that bind to G-protein-coupled receptors on the surfaces of cells of the opposite mating type, initiating a signaling cascade in which the βγ subunits of the G protein promote the activation of a mitogen-activated protein kinase (MAPK) cascade ( 4 ). (asm.org)
  • The yeast global transcriptional co-repressor protein Cyc8 can propagate as a prion. (brighthub.com)
  • Ssy1 is a plasma membrane protein ( 17 ) evolutionarily related to the amino acid permeases in S. cerevisiae ( 22 ) and thus is positioned at the presumed head of the amino acid signal transduction pathway. (asm.org)
  • The results of Western blot and ELISA confirmed that the 5hsCT protein had been expressed in the recombinant strain YS-5hsCT. (sigmaaldrich.com)
  • These yeast cells are immune to the toxic effects of the protein due to an intrinsic immunity. (wikipedia.org)
  • In S. cerevisiae are toxins encoded by a double-stranded RNA virus, translated to a precursor protein, cleaved and secreted outside of the cells, where they may affect susceptible yeast. (wikipedia.org)
  • The initial protein product from translation of the M dsRNA is called the preprotoxin, which is targeted to the yeast secretory pathway. (wikipedia.org)
  • [9] Other species of yeasts, such as Candida albicans , are opportunistic pathogens and can cause infections in humans. (wikipedia.org)
  • Torulaspora delbrueckii was formerly known as Saccharomyces delbrueckii or Saccharomyces rosei or Saccharomyces roseus, and the anamorph is called Candida colliculosa (for a complete list of synonyms, see CBS's website). (wikipedia.org)
  • Compare BREWERS' YEAST . (encyclopedia.com)
  • 6) Brewers use yeast to make alcohol. (bio.net)
  • The yeast Saccharomyces cerevisiae , known popularly as bakers ' or brewers ' yeast, has been used extensively in aging research. (encyclopedia.com)
  • Yeast, Saccharomyces cerevisiae, industrial yeast, laboratory yeast, hydrolysate, biotechnology INTRODUCTION Yeasts have served many important industrial purposes for humans over thousands of years. (deepdyve.com)
  • Strains with low G6PDH-activity grew slower in a lignocellulose hydrolysate than the strain with wild-type G6PDH-activity, which suggested that the availability of intracellular NADPH correlated with tolerance towards lignocellulose-derived inhibitors. (lu.se)
  • Product Features Taking high-nucleic acid Saccharomyces Cerevisiae as strain, molasses pure culture Saccharomyces Cerevisiae hydrolysate is a kind of bacterium made in such a. (digit-life.com)
  • Thousands of natural or genetically modified S. cerevisiae have been found in industrial environments for various purposes. (deepdyve.com)
  • This phenomenon is interesting in yeast evolution and may cause important sudden phenotype changes in genetically stable wine yeasts. (asm.org)
  • Therefore, in genetically unstable yeasts, the elimination of recessive lethal or deleterious alleles that decrease yeast fitness could occur rapidly in the absence of sporulation. (asm.org)
  • Even considering that all these phenomena may cause LOH and some "genome renewal," it seems likely that Mortimer's proposal ( 10 ) is the major mechanism for LOH in wild populations of genetically stable wine yeasts. (asm.org)
  • Thus, in some instances, two unrelated strains seemed to be genetically closer than two linked strains. (asm.org)
  • Yeasts are eukaryotic , single-celled microorganisms classified as members of the fungus kingdom . (wikipedia.org)
  • Maintenance of Quality Control Strains A guide to maintaining our KWIK-STIK and LYFO DISK microorganisms. (microbiologics.com)
  • Giving Saccharomyces boulardii to infants and children with diarrhea caused by rotavirus can reduce how long diarrhea lasts by about 1 day. (medlineplus.gov)
  • Giving Saccharomyces boulardii to infants with high levels of bilirubin in the blood doesn't decrease the length of time for which phototherapy is needed. (webmd.com)
  • First, we developed a new method, based on QPCR, to quantify the proportion of different Saccharomyces yeasts in mixed cultures. (frontiersin.org)
  • Concentrations of biomass and products in anaerobic batch cultures of different S. cerevisiae strains on glucose (20 g liter −1 ). (asm.org)
  • Torulaspora delbrueckii is now proposed as starter culture (to be associated with S. cerevisiae in mixed cultures) for certain applications, particularly to reduce volatile acidity in high-sugar fermentations like in Sauternes wines. (wikipedia.org)
  • The genome sequences of more than 100 strains of the yeast Saccharomyces cerevisiae have been published. (osti.gov)
  • Microsatellites and sequences of a selected set of loci able to recapitulate the yeast strain's evolutionary history were used to compare 17 environmental wasp isolates with a collection of strains from grapes from the same region and more than 230 strains representing worldwide yeast variation. (pnas.org)
  • Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenko Str. (hindawi.com)
  • Many studies of yeast have focused on the molecular mechanisms relevant to the utilization of nutrients [ 2 - 5 ]. (biomedcentral.com)
  • Background: Engineering of Saccharomyces cerevisiae for the simultaneous utilization of hexose and pentose sugars is vital for cost-efficient cellulosic bioethanol production. (rug.nl)
  • The sequencing of the yeast genome was a major milestone in biology. (brighthub.com)
  • Slightly higher amounts of glycerol were observed in samples fermented with strain EC1118 than in those fermented with strain RC212. (vtt.fi)
  • The wasp isolates fall into subclusters representing the overall ecological and industrial yeast diversity of their geographic origin. (pnas.org)
  • Seventeen Saccharomyces cerevisiae strains, including commercial wine yeast strains, were evaluated in laboratory-scale wine fermentations using natural Colombard grape must that contained similar amounts of glucose and fructose (approximately 110 g l −1 each). (wiley.com)
  • All strains showed preference for glucose, but to varying degrees. (wiley.com)
  • In Saccharomyces cerevisiae , distinct mechanisms have evolved to sense the extracellular presence of carbon sources such as glucose ( 19 , 20 , 23 , 24 ) and nitrogen sources including amino acids ( 6 , 13 , 16 , 17 ). (asm.org)
  • Consequently, engineered xylose-fermenting yeast strains first utilize D-glucose before D-xylose can be transported and metabolized. (rug.nl)
  • This resulted in D-glucose-tolerant growth of the yeast of D-xylose. (rug.nl)
  • Conclusions: Engineering of yeast endogenous hexose transporters provides an effective strategy to construct glucose-insensitive xylose transporters that are well integrated in the carbon metabolism regulatory network, and that can be used for efficient lignocellulosic bioethanol production. (rug.nl)
  • In recent decades, the yeast S. cerevisiae has been utilized extensively for biofuel production (Nissen et al.2000: 69-77). (deepdyve.com)
  • Albergaria and Arneborg, 2016 ), and S. cerevisiae is recognized as being the main microorganism responsible for this process ( Pretorius, 2000 ). (frontiersin.org)
  • Its distant cousin, Schizosaccharomyces pombe, or fission yeast, was shown in 2000 to undergo a very similar aging process. (encyclopedia.com)
  • The budding yeasts ("true yeasts") are classified in the order Saccharomycetales , [12] within the phylum Ascomycota. (wikipedia.org)
  • Vaughan-Martini, Cardinali and Martini (1996) used 24 strains of killer yeast from 13 species to find a resistance signature for each of 13 strains of S. cerevisiae which were used as starters in wine-making. (wikipedia.org)
  • Laboratory Evolution of a Biotin-Requiring Saccharomyces cerevisiae Strain for Full Biotin Prototrophy and Identification of Causal Mutations. (semanticscholar.org)
  • Third, yeast mutants were selected on the basis of a phenotype (property) frequently associated with aging. (encyclopedia.com)
  • Surprisingly few of these mutants (four strains) were affected for only one element. (biomedcentral.com)
  • However, recent studies of the yeast pheromone response pathway suggested that prior results with temperature-sensitive cdc42 mutants were misleading and that Cdc42p and the Cdc42p-PAK interaction are not involved in signaling. (asm.org)
  • Preston RA, Murphy RF, Jones EW (1989) Assay of vacuolar pH in yeast and identification of acidification-defective mutants. (springer.com)
  • Yeast mutants can be created and selected rapidly, again because it is a microbe producing many generations of progeny in a short time. (encyclopedia.com)
  • If you're a homebrewer or spirits enthusiast, you may know that yeast are responsible for converting simple sugars into, among other things, carbon dioxide and alcohol. (seriouseats.com)
  • The new yeast strains prepared by using recombinant DNA methods produce more α-galactosidase than naturally occurring α-galactosidase producing yeast strains. (freepatentsonline.com)
  • The ura3-P pgk -5hsCT sequence was introduced into the genome at rDNA sites by homologous recombination, and the recombinant strain YS-5hsCT was obtained. (sigmaaldrich.com)
  • Debaryomyces hansenii The susceptibility to toxins varies greatly between yeast species and strains. (wikipedia.org)
  • Among the three Saccharomyces cerevisiae DNA repair epistasis groups, the RAD6 group is the most complicated and least characterized, primarily because it consists of two separate repair pathways: an error-free postreplication repair pathway, and a mutagenesis pathway. (pnas.org)
  • Study of killer toxins helped to better understand the secretion pathway of yeast, which is similar to those of more complex eukaryotes. (wikipedia.org)
  • Other toxin systems are found in other yeasts: Pichia and Williopsis Hanseniaspora uvarum Zygosaccharomyces bailii Ustilago maydis: the smut fungus produces killer toxin Kp4 family fungal killer toxins. (wikipedia.org)
  • Abe F, Horikoshi K (1998) Analysis of intracellular pH in the yeast Saccharomyces cerevisiae under elevated hydrostatic pressure: a study in baro- (piezo-) physiology. (springer.com)
  • Respiratory petite colony mutant derived from ale yeast NCYC 239 treated with 50ppm acroflavine in the F.R.M. medium of kilkenny and Hinshelwood for 3hours @ 25C. (ncyc.co.uk)
  • The hpr1 Δ mutant of Saccharomyces cerevisiae was isolated in a screen for mutations that confer an increased mitotic recombination ( 1 , 2 ). (asm.org)
  • In addition, the ability of cdc15 diploid mutant strains to develop non-septated chains of cells, supported by nuclear division, is shown. (isciii.es)
  • After digestion by restriction endonuclease HpaI, a linear fragment, rDNA2-ura3-P pgk -5hsCT-rDNA1, was obtained and transformed into the △ura3 mutant of S. cerevisiae by the lithium acetate method. (sigmaaldrich.com)
  • Here, we describe the nearly complete genome sequence of GLBRCY22-3 (Y22-3), a strain of S. cerevisiae derived from the stress-tolerant wild strain NRRL YB-210 and subsequently engineered for xylose metabolism. (osti.gov)
  • Multilocus sequence typing of oenological Saccharomyces cerevisiae strains. (semanticscholar.org)
  • This study describes the application of a multilocus sequence typing (MLST) analysis for molecular discrimination at the strain level of Spanish wine yeast strains. (semanticscholar.org)
  • We generated 96 strains of C. glabrata by integrating 96 different sequence tags in the already disrupted URA3 locus ( 15 ). (sciencemag.org)
  • Interaction was followed in mixed fermentations with 1:1 inoculation of S. cerevisiae and ten non- Saccharomyces strains. (frontiersin.org)
  • The interaction was further analyzed using cell-free supernatant from S. cerevisiae and synthetic media mimicking both single fermentations with S. cerevisiae and using mixed fermentations with the corresponding non- Saccharomyces species. (frontiersin.org)
  • We observed that some cryotolerant Saccharomyces yeasts, particularly S. uvarum , seriously compromised S. cerevisiae fitness during competences at lower temperatures, which explains why S. uvarum can replace S. cerevisiae during wine fermentations in European regions with oceanic and continental climates. (frontiersin.org)
  • Finally, it is interesting to note that in co-inoculated fermentations, wine strains of S. cerevisiae and S. uvarum performed better than non-wine strains of the same species. (frontiersin.org)
  • dairy products' fermentations like traditional cheeses and fermented milk.Torulaspora delbrueckii can be an opportunistic spoilage yeast for dairy products or soft drinks (fruit juices, etc. (wikipedia.org)
  • Saccharomyces boulardii is most commonly used for treating and preventing diarrhea, including infectious types such as rotaviral diarrhea in children, diarrhea caused by gastrointestinal (GI) take-over (overgrowth) by "bad" bacteria in adults, traveler's diarrhea, and diarrhea associated with tube feedings. (webmd.com)
  • Taking Saccharomyces boulardii by mouth helps prevent diarrhea in people infected by Clostridium difficile bacteria. (webmd.com)
  • Taking Saccharomyces boulardii also seems to prevent diarrhea caused by this bacteria from re-occurring in some people. (webmd.com)
  • Using a pRS vector, one can perform most standard DNA manipulations in the same plasmid that is introduced into yeast. (genetics.org)
  • Using the plasmid pCnTel-1-labeled probe CENTEL, we were able to differentiate some unrelated strains that yielded the same hybridization profile with the C. neoformans middle-repetitive-element CNRE-1 probe. (asm.org)