Yeast gene order. Medical search

In the present study, we performed a genome-wide screening for sensitivity to cisplatin using non-essential haploid deletion mutants of the fission yeast Schizosaccharomyces pombe , belonging to a collection of haploid strains constructed through homologous recombination. (biomedcentral.com)
We measured the expression of 18 genes as function of time after addition of glucose to four strains of yeast grown in ethanol. (biomedcentral.com)
Our approach identifies gene groups that respond similarly to the change of nutrient, and genes that behave differently in mutant strains. (biomedcentral.com)
The technique also identifies genes that show perturbed expression in specific strains. (biomedcentral.com)
Based on these approaches, we were able to validate ARO1 , PDC1 , CPS1 , ASI2 , LYP1 , and ALP1 allelic variants underlying nitrogen consumption differences between strains, providing evidence of many genes with small phenotypic effects. (g3journal.org)
This method is based on driving yeast strains through repeated cycles of sexual assortment of deletions and fluorescence-based enrichment of cells carrying more deletions. (jove.com)
We have made a set of haploid and diploid strains (21,000) containing a complete deletion of each gene. (stanford.edu)
Food Technology, vol. 38 (Feb. 1984) No. 2, "Genetic manipulation of Brewing and Related Yeast Strains", Panchal, Russell, Sills and Stewart--pp. 99-106 & 111. (freepatentsonline.com)
Genetic Development of yeast strains"--Tubb, Sep. (freepatentsonline.com)
Analysis of other mutant strains has shown that the THI5 gene family members are functionally redundant, with each encoded isozyme having an apparent equal role in HMP formation from pyridoxine (vitamin B6). (bl.uk)
Physiological studies of these mutant strains have examined the regulation of each of the four genes by thiamin and its precursors, as well as investigating a recently proposed anaerobic HMP biosynthetic pathway. (bl.uk)
As bacteria reproduce by binary fission, homologous recombination between genes found among closely related strains was once thought to be rare, with periodic selection of rare advantageous mutations being a popular and fairly well-supported view. (asmscience.org)
Yeast Strains and Methods. (davidson.edu)
Here, we characterize YER067W , a conserved gene of unknown function that is strongly induced in response to many stress conditions and repressed in drug resistant yeast strains. (pubmedcentralcanada.ca)

The powerful tools of yeast genetics and cell biology have extended this description. (encyclopedia.com)
This has revolutionized yeast genetics. (encyclopedia.com)
Newswise - An international team of scientists led by Jef Boeke, PhD, director of NYU Langone Medical Center's Institute for Systems Genetics, has synthesized the first functional chromosome in yeast, an important step in the emerging field of synthetic biology, designing microorganisms to produce novel medicines, raw materials for food, and biofuels. (newswise.com)
Researchers say their team's global effort also marks one of the most significant advances in yeast genetics since 1996, when scientists initially mapped out yeast's entire DNA code, or genetic blueprint. (newswise.com)
Well-established genetics, high growth rates, a basic capacity for eukaryotic protein processing and secretion, and the "generally recognized as safe" (GRAS) status by the US Food and Drug Administration are just some of many beneficial traits of yeasts like S. cerevisiae . (springer.com)

Multiway real-time PCR gene expression profiling is a powerful technique which can be utilized to characterize functions of new genes by, for example, comparing their temporal response after perturbation in different genetic variants of the studied subject. (biomedcentral.com)
For example, although coordination of both gene expression and genetic linkage have been offered as genetic mechanisms for the frequently observed clustering of genes participating in fungal metabolic pathways, elucidation of the phenotype(s) favored by selection, resulting in cluster formation and maintenance, has not been forthcoming. (pnas.org)
Although both coordination of gene expression ( 5 , 6 , 11 ⇓ ⇓ ⇓ ⇓ - 16 ) and genetic linkage ( 7 , 17 ) have been offered as genetic mechanisms underlying these metabolic gene clusters, unlike the butterfly example above, the phenotype(s) that drives their formation and maintenance in fungi is not known. (pnas.org)
Further study found that many cereals are syntenic and thus plants such as rice or the grass Brachypodium could be used as a model to find genes or genetic markers of interest which could be used in wheat breeding and research. (wikipedia.org)
Several yeast genetic databases are accessible online, which facilitates functional genome analyses. (encyclopedia.com)
Delneri, D., Gardner, D. C. J. & Oliver, S. G. Analysis of the seven-member AAD gene set demonstrates that genetic redundancy in yeast may be more apparent than real. (nature.com)
Many microarray experiments have been designed to investigate the genetic mechanisms of cancer, and analytical approaches have been applied in order to classify different types of cancer or distinguish between cancerous and noncancerous tissue. (hindawi.com)
In the present experiments we examined whether these yeasts can exchange DNA and thereby create novel combinations of genetic material. (asm.org)
The resulting order of sequence-tagged sites is consistent with other physical and genetic mapping data. (nature.com)
At the coarsest resolution are genetic linkage maps, which depict the relative chromosomal locations of DNA markers (genes and other identifiable DNA sequences) by their patterns of inheritance. (lsu.edu)
Genes that are connected in such a way form genetic interaction networks . (wikipedia.org)
From this model genetic interactions can be observed at multiple scales which will assist in the study of concepts such as gene conservation. (wikipedia.org)
Paul Nurse also used the genetic approach in his cell cycle studies, but in a different kind of yeast. (nobelprize.org)
Certain diseases such as cystic fibrosis and muscular dystrophy are linked to genetic mutations that damage the important biological process of rearranging gene sequences in pre-messenger RNA, a procedure called RNA splicing. (redorbit.com)
Genetic manipulation of Snf1 and/or its targets has been a useful strategy for achieving various practical goals in yeast biotechnology, such as improved maltose utilization ( 12 ), galactose utilization and ethanol production ( 13 ), fatty acid production ( 14 , 15 ), and biomass yields ( 16 ). (asm.org)
Deletions can be a major driving force during genome reduction, whereby large portions--perhaps even the majority--of the genetic material of an organism are lost over time, with little gain of new genes. (asmscience.org)
1. A transformed living cell selected from the group consisting of fungi, yeast and bacteria, and containing genetic material derived from recombinant DNA material and coding for bovine rennin. (freepatentsonline.com)
This new integrative approach, known as "Genetical Genomics (GG)" or "Integrative Genomics", treats the expression level of each gene present on a microarray as a quantitative trait and use genetic markers to identify genomic regions that regulate gene expression phenotypes. (springer.com)
The genome shows a considerable amount of apparent genetic redundancy, and one of the major problems to be tackled during the next stage of the yeast genome project is to elucidate the biological functions of all of these genes. (sciencemag.org)
Explains the role of genes in health and disease, the basics of DNA and its molecular cousin RNA, and new directions in genetic research. (nih.gov)
HIS tall figure bent over a computer screen in his laboratory at the Massachusetts General Hospital, Dr. Gary Ruvkun rummages through a distant genetic data base for matches to a gene he believes is involved in diabetes. (nytimes.com)
But it seems to have the same genetic pathways -- systems of linked genes -- as those that go awry in human diabetes. (nytimes.com)
Genetic analyses in budding yeast have identified some of the genes involved in the spindle checkpoint pathway. (rupress.org)

In addition, the importance of lub1 gene emerged from the comparison between the present screening and gene expression profile data previously obtained in fission yeast. (biomedcentral.com)
Its distant cousin, Schizosaccharomyces pombe, or fission yeast, was shown in 2000 to undergo a very similar aging process. (encyclopedia.com)
Using a gene dosage approach, we have isolated the gene encoding HMG-CoA reductase, hmg1+ , from the fission yeast Schizosaccharomyces pombe (Accession Number L76979). (harvard.edu)
In this study, we have characterised fission yeast pop3-5235 , a diploidising mutant that is impaired in genome stability. (biologists.org)
For example, several fission yeast mutants defective in splicing reactions show cell-cycle specific phenotypes (Lundgren et al. (biologists.org)
U2AF consists of a large (Prp2 in fission yeast) and a small subunit, and it is the large subunit that is required for binding of the U2 snRNP to the branch point sequence (Parker et al. (biologists.org)
We investigated the role of the fission yeast bub1 gene in spindle checkpoint function and in unperturbed mitoses. (rupress.org)
We find that bub1 + is essential for the fission yeast spindle checkpoint response to spindle damage and to defects in centromere function. (rupress.org)

Yeast-like fungi of the human instestinal tract. (atcc.org)
There are major differences in the septin-associated processes of cytokinesis and deposition of new growth in budding yeast vs. filamentous fungi. (genetics.org)
Tetrad analysis in plants and fungi finds large differences in gene conversion rates but no GC bias. (bath.ac.uk)
A monophyletic group of black yeast-like fungi containing opportunistic pathogens around Exophiala spinifera is analyzed using sequences of the small-subunit (SSU) and internal transcribed spacer (ITS) domains of ribosomal DNA. (asm.org)
Like the majority of uncharacterized genes from S. cerevisiae , YER067W codes for a short protein (≤20 kDa) that is present in duplicate in the yeast genome (shares 70% identity with the ORF YIL057C ), and its homologs are restricted to fungi and contain no characterized functional domains . (pubmedcentralcanada.ca)

About 25 percent of human genes have yeast counterparts, and these human genes have frequently been shown to functionally replace the corresponding gene in the yeast cell. (encyclopedia.com)
Roughly half of the human genes could functionally replace their yeast counterparts. (sciencemag.org)
By moving human genes into yeast cells, in 1987 Nurse isolated a human cdc2 gene. (nobelprize.org)
Still, partial or full sequences of most human genes have already been deposited. (nytimes.com)

In the case of sensu stricto yeasts, stable viable hybrids were obtained. (asm.org)
The gene organization, including the gene order, seems to be rather conserved among the sensu stricto yeasts. (asm.org)
mtDNA of sensu stricto yeasts contains many guanine-cytosine clusters, and the size is greater than 60 kb. (asm.org)
Furthermore, the association and organisation with respect to neighbouring members of other subtelomeric gene families appears to be conserved between S. cerevisiae and other sensu stricto yeasts. (bl.uk)

Haploid cells carrying a disrupted GPA2 gene are viable. (pnas.org)
C. glabrata is a haploid yeast that contains three mating-type like loci ( MTL ), although no sexual cycle has been described. (scielo.br)
Candida glabrata is a haploid budding yeast that has only one known niche, the mammalian host and has recently emerged as a common opportunistic pathogen. (scielo.br)

Antisense transcripts and/or the act of antisense transcription regulate gene expression and genome integrity by interfering with sense transcription and modulating histone modifications or DNA methylation. (mdpi.com)
Furthermore, we concisely describe how antisense transcription/transcripts regulate gene expression and genome integrity with implications in disease pathogenesis and therapeutic development. (mdpi.com)
Non-coding antisense transcripts are generated from the strand opposite the sense strand and control sense transcription (and, therefore, gene expression). (mdpi.com)
Genes involved in signal transduction and transcription have been preferentially retained, and those involved in DNA repair have been preferentially lost. (plantcell.org)
Although the two members of each gene pair must originally have had identical transcription profiles, less than half of the pairs formed by the most recent polyploidy event still retain significantly correlated profiles. (plantcell.org)
Representative genes were verified using quantitative reverse transcription-polymerase chain reaction and NanoString analysis. (plantphysiol.org)
and characterising the role of specific yeast transcription factors in virulence in animal models. (ucd.ie)
By screening for transcription of a GAL4-dependent reporter gene, we identified a plasmid from the library carrying a fragment of the SIR4 gene. (davidson.edu)
In situ deletion or substitution of this sequence reduces both basal and induced transcription by at least two orders of magnitude. (nih.gov)
However, it is still in debate to what extent that common transcription factors (TFs) contribute to the co-expression of adjacent genes. (ebscohost.com)

Localization and deletion analyses of the histone H3 and CENH3 genes revealed that nuclear localization of the N-terminal tail is essential and sufficient for triggering the cell death phenotype. (frontiersin.org)
Cyanobacterium Synechococcus elongatus PCC 7942 holds promise for biochemical conversion, but gene deletion in PCC 7942 is time-consuming and may be lethal to cells. (biomedcentral.com)
In order to facilitate whole genome analysis each deletion is molecularly tagged with a unique 20-mer DNA sequence. (stanford.edu)
In contrast, genes required for acetyl-coenzyme A and lipid metabolism are upregulated in an ace2 deletion mutant grown predominantly as yeast cells but are downregulated in hyphae. (asm.org)

Essential genes in yeast are clustered, independent of co-expression and tandem duplication. (openwetware.org)
Clusters of essential genes are in regions of low recombination and larger clusters have lower recombination rates. (openwetware.org)
Critical ancestral functions of many essential genes are thus retained in a pathway-specific manner, resilient to drift in sequences, splicing, and protein interfaces. (sciencemag.org)
We speculate that there is an evolutionary drive to position essential genes in meiotic recombination coldspots for slow evolution, and genes that can afford to evolve more rapidly are placed near meiotic recombination hotspots. (prolekare.cz)

We show that, for MPH1 , the yeast homolog of Fanconi Anemia complementation group M ( FANCM ), the overexpression mutator phenotype is distinct from that of mph1 Δ. (genetics.org)
We report here a study in Schizosaccharomyces pombe to characterize a mutant ( cnp3-1 ) in a gene encoding a homolog of mammalian centromere-specific protein, CENP-C. At the restrictive temperature 36°, the Cnp3-1 mutant protein loses its localization at the centromere. (g3journal.org)
In budding yeast, Cse4, a homolog of mammalian CENP-A, is enriched at promoters that contain histone H2A ( Hildebrand and Biggins 2016 ). (g3journal.org)

We screened ∼5100 plasmids, each overexpressing a unique single gene, and characterized the five strongest mutators, MPH1 (mutator phenotype 1), RRM3 , UBP12 , PIF1 , and DNA2 . (genetics.org)
In order to uncover this, the researchers constructed DNA-rings (plasmids) containing one of the methyltransferases and "cassettes" holding multiple copies of certain DNA patterns. (eurekalert.org)
Multicopy episomal plasmids in yeast, used whenever elevated levels of foreign or homologous gene expression are necessary, are known to be less stable compared to the endogenous 2-μm plasmid they are based on, at least without selective pressure. (springer.com)
2013 ). YEp-type plasmids are present in multiple copies and thus promise a benefit from a gene-dosage effect in terms of an increased productivity (Romanos et al. (springer.com)
Interaction between the known protein and a protein encoded by one of the library plasmids leads to transcriptional activation of a reporter gene containing a binding site for GAL4. (davidson.edu)
J.N. Strathern and D.R. Higgins , Recovery of Plasmids from Yeast into Escherichia coli Shuttle Vectors. (elsevier.com)

In this study we were interested in exploring transcriptional responses of some of the less characterized ADH -genes to better understand their regulations under conditions of different glycolytic rates. (biomedcentral.com)
Transcriptional reprogramming is a fundamental process of living cells in order to adapt to environmental and endogenous cues. (biomedcentral.com)
Arrows (not to size) represent the order and transcriptional orientation of genes. (biologists.org)
We used this method with the yeast SIR4 protein, which is involved in the transcriptional repression of yeast mating type information. (davidson.edu)
The method is based on the properties of the yeast GAL4 protein, which consists of separable domains responsible for DNA-binding and transcriptional activation (5). (davidson.edu)
Our test case is the yeast SIR4 gene product, a protein involved in the transcriptional repression of the silent copies of mating type information (6). (davidson.edu)
Heat shock genes are poised for rapid transcriptional activation in response to environmental stress. (nih.gov)

Interestingly, we also found that overexpression of switchgrass histone H3 and CENH3 genes in N. benthamiana could trigger cell death of the transformed plant cells. (frontiersin.org)
To extend the catalog of genome instability genes, we systematically explored the effects of gene overexpression on mutation rate, using a forward-mutation screen in budding yeast. (genetics.org)
Overexpression of yeast HSF strongly suppresses the null phenotype of the induced hsp82-delta HSE1 gene and re-establishes DNase I hypersensitivity over its promoter. (nih.gov)

Marker & Reporter assays can be used to verify and quantify reporter gene products from expression vectors that have been introduced into cells or animals, such as lacZ or GFP. (thermofisher.com)
Marker & Reporter assays are designed to detect the copy number of marker and reporter genes from expression vectors that have been introduced into cells or animals, such as the lacZ reporter gene or antibiotic genes. (thermofisher.com)
J.C. Schneider and L. Guarente , Vectors for Expression of Cloned Genes in Yeast. (elsevier.com)
M. Schena, D. Picard, and K.R. Yamamoto , Vectors for Constitutive and Inducible Gene Expression in Yeast. (elsevier.com)
T.J. Koerner, J.E. Hill, A.M. Myers, and A. Tzagoloff , High-Expression Vectors with Multiple Cloning Sites for Construction of trpE Fusion Genes: pATH Vectors. (elsevier.com)

The coding parts of the mitochondrial genes within the sensu stricto group show less than 5% sequence diversity ( 5 ). (asm.org)
T.D. Fox, L.S. Folley, J.J. Mulero, T.W. McMullin, P.E. Thorsness, L.O. Hedin, and M.C. Costanzo , Analysis and Manipulation of Yeast Mitochondrial Genes. (elsevier.com)

Biclustering identifies subsets of genes and subsets of conditions that shares similar expression patterns, by performing simultaneous clustering of both rows and columns of the gene expression matrix. (biomedcentral.com)

The characterization of the isolated genes has provided a rich description of the aging process at the physiological level. (encyclopedia.com)
Here we describe characterization of a yeast larvicide developed through bioengineering of S. cerevisiae to express a short hairpin RNA (shRNA) targeting a conserved site in mosquito sema1a genes. (biomedcentral.com)

The gene, designated GPA1, encodes a protein (GP1 alpha) of 472 amino acids with a calculated Mr of 54,075. (pnas.org)
The gene encodes a membrane-bound cytochrome protein. (atlasgeneticsoncology.org)
They started with a bacterial gene, E. coli BirA , that encodes a biotin ligase-an enzyme that can attach biotin to specific acceptor peptides. (yeastgenome.org)
Researchers recently identified a new selfish gene, wtf4 , that encodes a toxin and an antitoxin in an effort to ensure the gene s own transmission by killing off reproductive cells lacking the gene. (nih.gov)
The PDGFB gene encodes the human platelet-derived growth factor (PDGF) B chain precursor and is the cellular homologue of the v-sis oncogene. (atlasgeneticsoncology.org)

Paleopolyploidy is detected by rather sophisticated bioinformatics tools that reveal similarity and colinearity between genes which diverged tens of millions of years ago (see Gaut, 2001 as example). (plantphysiol.org)
The similarity between the genes can be analyzed by clustering the gene expression data. (biomedcentral.com)

By evolving budding yeast after deleting an important component, we found that the perturbed module was repeatedly repaired by removing more components. (hfsp.org)
The module we investigated induces polarized cell growth in budding yeast. (hfsp.org)
In 2010, the most "complete" gene interactome produced to date was compiled from about 5.4 million two-gene comparisons to describe "the interaction profiles for ~75% of all genes in the budding yeast ", with ~170,000 gene interactions. (wikipedia.org)
Built-in Pol I elongation factors, such as Rpa34/Rpa49 in budding yeast and PAF53/CAST in humans, are instrumental to the extremely high rate of rRNA production per gene. (hindawi.com)
Observation of cryofixed cryosubstituted other sections analyzed by electron microscopy reveals that exponentially growing budding yeast cells contain up to 10 4 ribosomes per μ m 3 [ 1 ], which represents up to 10% of the cytoplasmic volume [ 2 ] (Figure 1(a) ). (hindawi.com)
Budding yeast cells and ribosome production. (hindawi.com)
Epigenetic silencing mechanisms in budding yeast and fruit fly: different paths, same destinations. (prolekare.cz)
Dynamic Properties of Cell-cycle and Life-cycle networks in Budding Yeast. (nhbs.com)

This is done to regulate gene expression, distinguish indigenous DNA from foreign DNA, or to mark old DNA strands during replication. (eurekalert.org)

These assays are specifically designed to detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters. (thermofisher.com)
Expression of glycolytic enzymes is reduced, while expression of respiratory genes (including those involved in the tricarboxylic acid cycle, oxidative phosphorylation, and ATP synthesis) is increased. (asm.org)
Synthesis of the large rRNA precursors (35-47S) can be achieved by up to 150 RNA polymerase I (Pol I) enzymes simultaneously transcribing each rRNA gene. (hindawi.com)
Analysis of transcribed ribosomal DNA (rDNA) after Miller spreading revealed that up to 150 Pol I enzymes simultaneously transcribe rRNA genes in mutant with only 25 rRNA genes [ 1 , 7 ] (Figures 1(c) and 1(d) ). (hindawi.com)

Seoighe, C. & Wolfe, K. H. Yeast genome evolution in the post-genome era. (nature.com)
My recent bench research has focused on using whole-genome DNA and RNA sequencing, ChIP-Seq, array-CGH, and other "omics" methods to broadly explore evolution in yeast, and particularly the genome structures and genome evolution of industrial yeasts (lager, ale, wine, ethanol, bread). (stanford.edu)

Our work reveals that MMR components are required for stable inheritance of gene silencing patterns and establishes a link between the MMR machinery and the control of epigenetic silencing. (prolekare.cz)

M.D. Rose and J.R. Broach , Cloning Genes by Complementation in Yeast. (elsevier.com)

Adjacent pairs of genes show correlated expression independent of their orientation. (openwetware.org)
Neighboring genes show correlated evolution in gene expression. (bath.ac.uk)

In an extended 18S ribosomal DNA (rDNA) sequencing study of black yeasts and their allies, Haase et al. (asm.org)
Ribosomal RNA genes shape chromatin domains associating with the nucleolus. (bioportfolio.com)

Thus, individual cells are mortal, while the yeast population is immortal. (encyclopedia.com)
In practical terms, yeast life span is measured by observing individual cells periodically under a microscope and removing buds with a micro-manipulator. (encyclopedia.com)
Several procedures for the bulk preparation of age-synchronized yeast cells are available. (encyclopedia.com)
They behave almost identically to wild yeast cells, only they now possess new capabilities and can do things that wild yeast cannot. (newswise.com)
It's known that gene transfer occurs between different bacteria and even between bacteria and yeast cells. (infectioncontroltoday.com)
C. glabrata cells however, are able to maintain cell type identity through differential regulation of expression of the α genes and differential splicing of the a1 gene transcript (Muller et al. (scielo.br)
Flow cytometry with rhodamine 6G suggested an increased drug efflux in mutant cells, which was further supported by Northern blot analysis of the expression of the C. glabrata CDR1 ( CgCDR1 ) and CgCDR2 genes, encoding efflux pumps. (asm.org)
Similar changes occur in both yeast and hyphal cells. (asm.org)
This human cdc2 gene functioned perfectly in yeast cells. (nobelprize.org)
Thus, while prolonged glucose deprivation might seem to put yeast cells "on their knees," concomitant accumulation of Reg2 helps configure the cells into a "sprinter's crouch start position" to spring into action once glucose becomes available. (asm.org)
Heterologous expression of the BnFatB gene in yeast cells was performed in order to ascertain the function of the BnFatB gene. (magiran.com)
Moreover, knockdown of MMR components in human HEK293T cells increased subtelomeric DUX4 gene expression. (prolekare.cz)
The gentle and careful in vitro processing of live cells is essential in order to make them available to future therapeutic applications. (biomedsearch.com)
Just like the camera hidden in the jungle, the tags didn't perturb yeast cells in the least. (yeastgenome.org)
He has found two families of genes that are involved in relaying messages about sugar metabolism to the information center in the nucleus of living cells. (nytimes.com)
These scientific approaches focus no longer on individual units, such as nerve cells or genes, but rather on the emerging dynamic patterns of interactions between them. (nhbs.com)

Experiments conducted on Aedes aegypti larvae demonstrated that the yeast larvicide effectively silences sema1a expression, generates severe neural defects, and induces high levels of larval mortality in laboratory, simulated-field, and semi-field experiments. (biomedcentral.com)
Defects in any of these genes could be contributory causes of the disease, he believes, and if so, biologists trying to trace the pedigrees of diabetic genes have a whole new range of targets to consider. (nytimes.com)

RNA-seq data derived from lowland cultivar Alamo and upland cultivar Dacotah allowed us to identify SNPs in the histone H3 genes and compare their differential gene expression. (frontiersin.org)
The selected pathways were used to design panels of real-time PCR primers tailored for the top-ranked genes for differential gene expression analysis. (bio-rad.com)

This is largely due to the process of duplicate gene reduction, eventually affecting most pairs of duplicate genes created by the WGD , which distributes the surviving members of duplicate pairs between two homeologous chromosomal segments in an unpredictable way, fractionation [ 15 - 19 ], thus scrambling gene order and disrupting the phylogenetic signal. (biomedcentral.com)

To extract maximum information from profiling experiments using such panels, methods to pre-process and process the gene expression data are needed. (biomedcentral.com)
further emphasizing the need for methods to identify functionally relevant genes for tumor biology and progression within SCNAs. (genetics.org)
Ancestral gene order reconstruction for flowering plants has lagged behind developments in yeasts, insects and higher animals, because of the recency of widespread plant genome sequencing, sequencers' embargoes on public data use, paralogies due to whole genome duplication ( WGD ) and fractionation of undeleted duplicates, extensive paralogy from other sources, and the computational cost of existing methods. (biomedcentral.com)
Microarray meta-analysis using 13 microarray experiments combined with empirically defined filtering criteria identified a set of 226 genes differentially regulated by cytokinin, a subset of which has previously been validated by other methods. (plantphysiol.org)
These data sets have identified many genes regulated by cytokinin, and several have been validated by other methods ( Supplemental Table S1 ). (plantphysiol.org)
We have developed methods for generating gene knockouts in Candida parapsilosis, and we are currently constructing a set of individual knockouts. (ucd.ie)

Paper apparently describes a yeast mutant in which a 10kb linear YAC is stably maintained. (openwetware.org)
moreover, transcript levels of genes other than the α-tubulin gene are also equally decreased in this mutant. (biologists.org)
From Cloned Gene to Mutant Yeast. (elsevier.com)

How far across evolution do families of genes retain their function? (sciencemag.org)

RNA-seq analysis identified 73 cytokinin-regulated genes that were not represented on the ATH1 microarray. (plantphysiol.org)
Gene expression microarray is a highly popular technology that allows genome-wide measurement of RNA expression levels in a highly quantitative manner. (biomedcentral.com)
Cluster analysis plays an important role in the microarray data analysis through the grouping of genes into subsets with similar expression patterns or similar function. (biomedcentral.com)

mRNA levels of 660 genes were correlated with the AF trait. (springer.com)

The selectable marker gene GEN3 was amplified with primers S1 and S2 providing the homology region for homologous recombination (black boxes). (biologists.org)

G.F. Sprague, Jr. , Assay of Yeast Mating Reaction. (elsevier.com)

However, we also show that deleting ace2 results in downregulation of expression of glycolytic genes and in upregulation of expression of many genes required for mitochondrial function and that Ace2 is required for filamentation in response to hypoxic conditions. (asm.org)

Alcoholic fermentation is exclusively carried out by yeasts and the process transforms sugar within the grape must into ethanol and CO 2 . (g3journal.org)
This invention provides a process for producing ethanol from a starchy material, wherein the starchy material is simultaneously subjected to saccharification and subsequent fermentation by use of a yeast host which has been transformed by an expressible recombinant vector comprising a glucoamylase gene derived from a fungus of the genus Rhizopus. (freepatentsonline.com)
The advanced Innova yeast allows producers to maximize inputs, achieve throughput, and production targets, without losing ethanol yield to common stressors such as high temperature. (biofuelsdigest.com)

Let's explore today - using the recent news from Novozymes about their pioneering advances in yeast - what exactly a breakthrough is, and does. (biofuelsdigest.com)

The remaining two genes were found to be homologous to CENH3 . (frontiersin.org)
To date, S. pombe is the only unicellular eukaryote that has been found to contain a single HMG-CoA reductase gene. (harvard.edu)
Moreover, a large fraction (∼80%) of the predicted yeast proteome has been found to be expressed under normal laboratory growth conditions, with high-throughput tagging or MS-based proteomics ( 16 ⇓ - 18 ). (mcponline.org)
Novel splicing events were found in members of some gene families that are known to play a role in cytokinin signaling or metabolism. (plantphysiol.org)
Here, addition of a pentameric repeat to a region found in-frame will cause the downstream region of the gene to be translated out-of-frame, resulting in a useless protein or premature translation termination. (asmscience.org)
These DNA primer pairs were designed by prioritizing the gene regions most commonly found in transcript variants. (bio-rad.com)
We further found that the identified genes were also required for proper silencing in telomeres. (prolekare.cz)
The single teleomorph genus in the order, Capronia , was found throughout the tree but appeared to have limited clinical relevance. (asm.org)
We further proposed a case of the PAC (polymerase A and C) and RRPE (rRNA processing element) motifs which co-regulate divergent/bidirectional pairs, and found that the shared TFs were not significantly relevant to co-expression of divergent promoters among adjacent genes. (ebscohost.com)

To achieve maximal flux channelling toward target products, the regulation of expression of multiple genes is desirable, which is labor intensive and time consuming in most cases. (biomedcentral.com)

Thus, the CDK function in the cell cycle engine had been concerved through more than one billion years of evolution - from yeast to man. (nobelprize.org)
Inspired by this work, we hope to engineer yeast to make an in vivo evolution machine. (igem.org)
Polygenic evolution of a sugar specialization tradeoff in yeast. (buckinstitute.org)
Therefore we believe that during evolution yeasts have developed a sophisticated regulatory system that integrates both TF-based and non-TF based mechanisms(s) for concurrent regulation of neighboring genes in response to various environmental changes. (ebscohost.com)

Anatomy18

Organisms26

Diseases3

Chemicals and Drugs73

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes121

Disciplines and Occupations3

Technology, Industry, Agriculture2

Information Science8

Health Care4

Mad2

Christina Smolke

Protein inhibitor of activated STAT

Genotoxicity

Paleopolyploidy

Synteny

Protein engineering

YCL064C

DNA replication factor CDT1

Schizosaccharomyces pombe

Kenneth H. Wolfe

Alison Woollard

Functional genomics

ANLN

Cohesin

Ziv Bar-Joseph

Chaetomium thermophilum

RNA polymerase II

ALG1

Leptomycin

Callystatin A

Interactome

Protein

Smash and Grab (biology)

Phosphoribosylamine-glycine ligase

Binarization of consensus partition matrices

Cell cycle

Microbial genetics

ILCD

Orders of magnitude (mass)

Quantitative trait locus

Oligosaccharyltransferase

Kluyveromyces marxianus

Sake

Refractive index

Whale

Pleiotropy

TATA box

Histone

Biofuel

Fluorescein

Pu'er tea

Pasteur Institute

Superoxide dismutase

Oat

Amyotrophic lateral sclerosis

Proteasome

효소 - 위키백과, 우리 모두의 백과사전

Vitamin C

Flea

Autophagy

Vitamin C

Histone methyltransferase

Protein

Single cell sequencing

Herbalism

Louis Pasteur

Sociobiology: The New Synthesis

Phenolic content in wine

Mitosis

Yeast Gene Order Browser - User Guidelines

Difference between revisions of "Yeast rebuild" - OpenWetWare

Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein:...

RPL27A - 60S ribosomal protein L27-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - RPL27A gene &...

PGM2 - Phosphoglucomutase 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - PGM2 gene & protein

Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast | BMC Genomics | Full Text

Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of...

Physical linkage of metabolic genes in fungi is an adaptation against the accumulation of toxic intermediate compounds | PNAS

Candida glabrata (Anderson) Meyer et Yarrow ATCC ® 2001™

ncRNA | Free Full-Text | Mechanisms of Antisense Transcription Initiation with Implications in Gene Expression, Genomic...

Frontiers | Identification and Characterization of Switchgrass Histone H3 and CENH3 Genes | Plant Science

Stuecker TN[au] - PubMed - NCBI

Best PhD Thesis Topics on Biology | A Listly List

Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase | Genetics

Characterization of the Aspergillus nidulans Septin (asp) Gene Family | Genetics

Frontiers | Multiple Duties for Spindle Assembly Checkpoint Kinases in Meiosis | Cell and Developmental Biology

Synteny - Wikipedia

Yeast | Encyclopedia.com

Identification of Nitrogen Consumption Genetic Variants in Yeast Through QTL Mapping and Bulk Segregant RNA-Seq Analyses | G3:...

Scientists Synthesize First Functional "Designer" Chromosome in Yeast | Newswise: News for Journalists

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