Wnt Proteins: Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.Wnt Signaling Pathway: A complex signaling pathway whose name is derived from the DROSOPHILA Wg gene, which when mutated results in the wingless phenotype, and the vertebrate INT gene, which is located near integration sites of MOUSE MAMMARY TUMOR VIRUS. The signaling pathway is initiated by the binding of WNT PROTEINS to cells surface WNT RECEPTORS which interact with the AXIN SIGNALING COMPLEX and an array of second messengers that influence the actions of BETA CATENIN.Wnt3A Protein: A Wnt protein subtype that plays a role in cell-cell signaling during EMBRYONIC DEVELOPMENT and the morphogenesis of the developing NEURAL TUBE.Wnt3 Protein: A Wnt protein subtype that plays a role in cell-cell signaling during EMBRYONIC DEVELOPMENT and the morphogenesis of the developing NEURAL TUBE. Defects in Wnt3 protein are associated with autosomal recessive tetra-AMELIA in humans.Wnt1 Protein: A proto-oncogene protein and member of the Wnt family of proteins. It is expressed in the caudal MIDBRAIN and is essential for proper development of the entire mid-/hindbrain region.Wnt4 Protein: A Wnt protein that is involved in regulating multiple developmental processes such as the formation of the KIDNEY; ADRENAL GLANDS; MAMMARY GLANDS; the PITUITARY GLAND; and the female reproductive system. Defects in WNT4 are a cause of ROKITANSKY KUSTER HAUSER SYNDROME.Wnt2 Protein: A proto-oncogene protein and member of the Wnt family of proteins. It is frequently up-regulated in human GASTRIC CANCER and is a tumor marker (BIOMARKERS, TUMOR) of gastric and COLORECTAL CANCER.Receptors, Wnt: Cell-surface receptors that specifically bind to WNT PROTEINS and activate the WNT SIGNALING PATHWAY.Frizzled Receptors: A family of seven-pass transmembrane cell-surface proteins that combines with LOW DENSITY LIPROTEIN RECEPTOR-RELATED PROTEIN-5 or LOW DENSITY LIPROTEIN RECEPTOR-RELATED PROTEIN-5 to form receptors for WNT PROTEINS. Frizzled receptors often couple with HETEROTRIMERIC G PROTEINS and regulate the WNT SIGNALING PATHWAY.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Low Density Lipoprotein Receptor-Related Protein-6: An LDL-receptor related protein that combines with cell surface FRIZZLED RECEPTORS to form WNT PROTEIN-binding receptors. The protein plays an important role in the WNT SIGNALING PATHWAY during EMBRYONIC DEVELOPMENT and in regulation of vascular cell proliferation.Zebrafish Proteins: Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).Axin Protein: A scaffolding protein that is a critical component of the axin signaling complex which binds to ADENOMATOUS POLYPOSIS COLI PROTEIN; GLYCOGEN SYNTHASE KINASE 3; and CASEIN KINASE I.TCF Transcription Factors: A family of DNA-binding proteins that are primarily expressed in T-LYMPHOCYTES. They interact with BETA CATENIN and serve as transcriptional activators and repressors in a variety of developmental processes.Gene Expression Regulation, Developmental: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.LDL-Receptor Related Proteins: A family of proteins that share sequence similarity with the low density lipoprotein receptor (RECEPTORS, LDL).Lymphoid Enhancer-Binding Factor 1: A T-cell factor that plays an essential role in EMBRYONIC DEVELOPMENT.Intercellular Signaling Peptides and Proteins: Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.Receptor Tyrosine Kinase-like Orphan Receptors: A family of cell surface receptors that were originally identified by their structural homology to neurotropic TYROSINE KINASES and referred to as orphan receptors because the associated ligand and signaling pathways were unknown. Evidence for the functionality of these proteins has been established by experiments showing that disruption of the orphan receptor genes results in developmental defects.Low Density Lipoprotein Receptor-Related Protein-5: LDL-receptor related protein that combines with FRIZZLED RECEPTORS at the cell surface to form receptors that bind WNT PROTEINS. The protein plays an important role in the WNT SIGNALING PATHWAY in OSTEOBLASTS and during EMBRYONIC DEVELOPMENT.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Xenopus Proteins: Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.Body Patterning: The processes occurring in early development that direct morphogenesis. They specify the body plan ensuring that cells will proceed to differentiate, grow, and diversify in size and shape at the correct relative positions. Included are axial patterning, segmentation, compartment specification, limb position, organ boundary patterning, blood vessel patterning, etc.Cytoskeletal Proteins: Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.Embryo, Nonmammalian: The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.Zebrafish: An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Transcription Factor 7-Like 2 Protein: A transcription factor that takes part in WNT signaling pathway. The activity of the protein is regulated via its interaction with BETA CATENIN. Transcription factor 7-like 2 protein plays an important role in the embryogenesis of the PANCREAS and ISLET CELLS.Mesoderm: The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.T Cell Transcription Factor 1: A TCF transcription factor that was originally identified as a DNA-binding protein that interacts with the enhancers of T-CELL RECEPTOR ALPHA GENES. It plays a role in T-LYMPHOCYTE development.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Receptors, G-Protein-Coupled: The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS.Xenopus: An aquatic genus of the family, Pipidae, occurring in Africa and distinguished by having black horny claws on three inner hind toes.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Adaptor Proteins, Signal Transducing: A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymesGastrulation: A process of complicated morphogenetic cell movements that reorganizes a bilayer embryo into one with three GERM LAYERS and specific orientation (dorsal/ventral; anterior/posterior). Gastrulation describes the germ layer development of a non-mammalian BLASTULA or that of a mammalian BLASTOCYST.Adenomatous Polyposis Coli Protein: A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.Morphogenesis: The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.Bone Morphogenetic Proteins: Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.Homeodomain Proteins: Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Cell Line, Tumor: A cell line derived from cultured tumor cells.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Lithium Chloride: A salt of lithium that has been used experimentally as an immunomodulator.Hedgehog Proteins: A family of intercellular signaling proteins that play and important role in regulating the development of many TISSUES and organs. Their name derives from the observation of a hedgehog-like appearance in DROSOPHILA embryos with genetic mutations that block their action.Cell Lineage: The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.HEK293 Cells: A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Gastrula: The developmental stage that follows BLASTULA or BLASTOCYST. It is characterized by the morphogenetic cell movements including invagination, ingression, and involution. Gastrulation begins with the formation of the PRIMITIVE STREAK, and ends with the formation of three GERM LAYERS, the body plan of the mature organism.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Cell Polarity: Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.PhosphoproteinsGlycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Embryonic Induction: The complex processes of initiating CELL DIFFERENTIATION in the embryo. The precise regulation by cell interactions leads to diversity of cell types and specific pattern of organization (EMBRYOGENESIS).Ectoderm: The outer of the three germ layers of an embryo.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Embryonic Development: Morphological and physiological development of EMBRYOS.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Animals, Genetically Modified: ANIMALS whose GENOME has been altered by GENETIC ENGINEERING, or their offspring.Gene Knockdown Techniques: The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Wnt7b is expressed in maturing dendrites, and the expression of the Wnt receptor Frizzled (Fz), increases highly with synapse ... In the vertebrate NMJ, motor neuron expression of Wnt-11r contributes to acetylcholine receptor (AChR) clustering in the ... Wnt-5a performs a similar function on postsynaptic granule cells; this Wnt stimulates receptor assembly and clustering of the ... Wnt is needed to localize glutamatergic receptors on postsynaptic muscle cells. As a result, Wnt mutations diminish evoked ...
Wingless acts on engrailed-expressing cells to stabilize the stripes of engrailed expression. Wingless is a member of the Wnt ... The frizzled family of WNT receptors have some sequence similarity to Smoothened. However, G proteins have been difficult to ... acts to prevent high expression and activity of a 7 membrane spanning receptor called Smoothened (SMO). Patched has sequence ... Aspiras, A.C.; Prasad, R.; Fong, D.W.; Carlini, D.B.; Angelini, D.R. (2012). "Parallel reduction in expression of the eye ...
... gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The expression of the FZD2 ... "Entrez Gene: FZD2 frizzled homolog 2 (Drosophila)". "Frizzled Receptors: FZD2". IUPHAR Database of Receptors and Ion Channels. ... 2004). "The receptor tyrosine kinase Ror2 is involved in non-canonical Wnt5a/JNK signalling pathway". Genes Cells. 8 (7): 645- ... 1997). "Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action". Proc. Natl. Acad. Sci. U. ...
"The BCL9-2 proto-oncogene governs estrogen receptor alpha expression in breast tumorigenesis". Oncotarget. 5 (16): 6770-87. doi ... "Identification of a link between Wnt/β-catenin signalling and the cell fusion pathway". Nat Commun. 2: 548. doi:10.1038/ ... 141 (4): 1359-70, 1370.e1-3. doi:10.1053/j.gastro.2011.06.039. PMID 21703997. Matsuura K, Jigami T, Taniue K, Morishita Y, ...
... differential expression of membrane receptors and ligands that cause lateral inhibition, in which the receptor-expressing cell ... concentration gradients of secreted proteins across the embryo such as Wnt, Nodal, and Bone Morphogenic Proteins (BMPs), and 4 ... Tabuse Y, Izumi Y, Piano F, Kemphues KJ, Miwa J, Ohno S (September 1998). "Atypical protein kinase C cooperates with PAR-3 to ... 3 (5): 362-374. doi:10.1038/nrc1075. PMID 12724734. Wedlich-Soldner, Roland; Li, Rong (2003-04-01). "Spontaneous cell ...
"Elevated expression of Wnt antagonists is a common event in hepatoblastomas". Clinical Cancer Research. 11 (12): 4295-304. doi: ... "Increased expression of the E3-ubiquitin ligase receptor subunit betaTRCP1 relates to constitutive nuclear factor-kappaB ... Elevated levels of βTrCP expression have been found in colorectal, pancreatic, hapatoblastoma, and breast cancers. GRCh38: ... an E3 ubiquitin ligase receptor, beta-catenin, and NF-kappaB in colorectal cancer". Journal of the National Cancer Institute. ...
Ji J, Yamashita T, Wang XW (2011). "Wnt/β-catenin signaling activates microRNA-181 expression in hepatocellular carcinoma". ... "Suppression of retinoid X receptor alpha and aberrant β-catenin expression significantly associates with progression of ... Stimulation of the Wnt/β-catenin pathway, and its role in promoting malignant tumor formations and metastases, has also been ... It has been shown that HIF-1α can induce the EMT pathway, as well as the Wnt/β-catenin signaling pathway, thus enhancing the ...
... is a key component of the LRP5/LRP6/Frizzled co-receptor group that is involved in canonical Wnt pathway. Mutations in ... Papathanasiou I, Malizos KN, Tsezou A (Mar 2010). "Low-density lipoprotein receptor-related protein 5 (LRP5) expression in ... He X, Semenov M, Tamai K, Zeng X (Apr 2004). "LDL receptor-related proteins 5 and 6 in Wnt/beta-catenin signaling: arrows point ... LRP5 acts as a co-receptor with LRP6 and the Frizzled protein family members for transducing signals by Wnt proteins through ...
LGR4 and LGR5 are R-spondin receptors mediating Wnt/β-catenin and Wnt/PCP signalling. EMBO Rep. 2011 Sep 30;12(10):1055-61. ... Murayama M, Okamoto R, Tsuchiya K, Akiyama J, Nakamura T, Sakamoto N, Kanai T, Watanabe M. Musashi-1 suppresses expression of ... Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature. 2011 Jul 4;476(7360):293-7. Garg P, Ravi ... Rapid loss of intestinal crypts upon conditional deletion of the Wnt/Tcf-4 target gene c-Myc. Mol Cell Biol. 2006 Nov;26(22): ...
The Wnt protein triggers expression of the EphB receptors deep within the crypt, leading to decreased Eph expression and ... Eph receptors in turn compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Since ephrin ligands ( ... though regulation of angiogenesis by mitigating expression in the VEGF signalling pathway. Ephrin-B2 affects VEGF-receptors (e. ... Eph receptors in turn are classified as either EphAs or EphBs based on their binding affinity for either the ephrin-A or ephrin ...
Gene expression profiling revealed the prevalence of specific fibroblast growth factors (FGFs) and FGF receptors in NSCLC cell ... These findings suggest that interference with Wnt signaling at the ligand-receptor level may improve the effectiveness of ... For example, despite widespread expression of epidermal growth factor receptors (EGFRs) and EGF family ligands in non-small- ... For example, autocrine Wnt signaling could provide a novel target for therapeutic intervention by means of Wnt antagonists or ...
... and an inhibitor of Wnt / beta-catenin. It suggests that miR-181 may eradicate HCC. The increased expression of miR-181a in ... "MicroRNA signatures predict oestrogen receptor, progesterone receptor and HER2/neu receptor status in breast cancer". Breast ... miR-181 expression has a reverse correlation with Tcl1 protein expression. mir-181 a and b are over-expressed and act as bad ... miR-181a expression is associated with survival in triple negative breast cancer. Patients with low expression have lower ...
2009). "The Wnt receptor FZD1 mediates chemoresistance in neuroblastoma through activation of the Wnt/beta-catenin pathway". ... 2007). "Expression of seven gastric cancer-associated genes and its relevance for Wnt, NF-kappaB and Stat3 signaling". APMIS. ... 2005). "Correlation of Wnt-2 expression and beta-catenin intracellular accumulation in Chinese gastric cancers: relevance with ... 2005). "Substantial changes in gene expression of Wnt, MAPK and TNFalpha pathways induced by TGF-beta1 in cervical cancer cell ...
"Restoration of Wnt-7a expression reverses non-small cell lung cancer cellular transformation through frizzled-9-mediated growth ... "Frizzled Receptors: FZD9". IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical ... Karasawa T, Yokokura H, Kitajewski J, Lombroso PJ (Oct 2002). "Frizzled-9 is activated by Wnt-2 and functions in Wnt/beta - ... "A novel human homologue of the Drosophila frizzled wnt receptor gene binds wingless protein and is in the Williams syndrome ...
Expression levels of miR-27 are positively correlated with beta-catenin, a key protein in Wnt signalling. There is activation ... "MicroRNA-27a Indirectly Regulates Estrogen Receptor {alpha} Expression and Hormone Responsiveness in MCF-7 Breast Cancer Cells ... FOXO1 protein expression is down-regulated in breast tumour tissue samples; miR-27a has been identified as one of three miRNAS ... mir-27b expression is associated with survival in triple negative breast cancer patients. Inhibition of miR-27 by antisense ...
"The Wnt receptor FZD1 mediates chemoresistance in neuroblastoma through activation of the Wnt/beta-catenin pathway". Oncogene. ... "Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human ... "WNT-4 mRNA expression in human adrenocortical tumors and cultured adrenal cells". Hormone and Metabolic Research = Hormon- Und ... "Variation in WNT genes expression in different subtypes of chronic lymphocytic leukemia". Leukemia & Lymphoma. 50 (12): 2061-70 ...
... a receptor gene for Wnt ligands, is associated with the susceptibility to schizophrenia". Neurosci. Lett. 353 (1): 53-6. doi: ... Sala CF, Formenti E, Terstappen GC, Caricasole A (Jul 2000). "Identification, gene structure, and expression of human frizzled- ... "Frizzled Receptors: FZD3". IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical ... Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. It may play a role in mammalian hair ...
Expression of UNC-6 netrin and its receptor UNC-5 is required for guiding pioneering axons and migrating cells in C. elegans. ... Extracellular cues such as Wnt fibroblast growth factor can promote synapse formation, contradicting the traditional view of ... Manitt, Colleen; Thompson, Katherine M.; Kennedy, Timothy E. (2004). "Developmental shift in expression of netrin receptors in ... The binding of netrin-1 to its receptors inhibits the tumor suppressor p53 dependent apoptosis. Such receptors share the ...
June 2005). "Elevated expression of Wnt antagonists is a common event in hepatoblastomas". Clin. Cancer Res. 11 (12): 4295-304 ... "Epidermal Growth Factor Receptor: A Novel Target of the Wnt/β-Catenin Pathway in Liver". Gastroenterology. 129 (1): 285-302. ... Recently on other components of the Wnt signaling pathway have also demonstrated a likely role for constitutive activation of ... receptors". The Journal of Biological Chemistry. 277 (34): 30656-64. doi:10.1074/jbc.M203597200. PMID 12065588. Ang JP, Heath ...
... is a key component of the LRP5/LRP6/Frizzled co-receptor group that is involved in canonical Wnt pathway. LRP6 is a ... 2005). "LRP6 expression promotes cancer cell proliferation and tumorigenesis by altering beta-catenin subcellular distribution ... He X, Semenov M, Tamai K, Zeng X (2004). "LDL receptor-related proteins 5 and 6 in Wnt/beta-catenin signaling: arrows point the ... LRP6 acts as a co-receptor with LRP5 and the Frizzled protein family members for transducing signals by Wnt proteins through ...
... 1 has highest expression in cotyledons and flowers while Epsin2 and Epsin3 expression is currently unknown. Little is ... There is also evidence that Epsin could lead to colon cancer through impaired Wnt Signaling by reducing the stability of the ... A genetic abnormality in CLINT1 is assumed to change the way internalisation of neurotransmitter receptors occurs in the brains ... In mammals, the two main classes of Epsin's are expressed throughout tissues but has the highest expression in the brain, ...
2005). "Frizzled-7 receptor ectodomain expression in a colon cancer cell line induces morphological change and attenuates tumor ... Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The ... "Frizzled Receptors: FZD7". IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical ... FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human ...
2005). „Restoration of Wnt-7a expression reverses non-small cell lung cancer cellular transformation through frizzled-9- ... Kalcijum-detektujući receptor • GABA B (1, 2) • Glutamatni receptor (Metabotropni glutamat (1, 2, 3, 4, 5, 6, 7, 8)) • GPRC6A ... Frizzled Receptors: FZD9". IUPHAR Database of Receptors and Ion Channels. International Union of Basic and Clinical ... Karasawa T, Yokokura H, Kitajewski J, Lombroso PJ (2002). „Frizzled-9 is activated by Wnt-2 and functions in Wnt/beta -catenin ...
Extracellular Wnt signalling acts on the Frizzled receptor on the cellular membrane, which then, through a signalling cascade ... Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T (2003). "Construction of expression-ready cDNA clones for KIAA ... MACF1 was shown to be present in the Wnt signaling pathway. When Wnt signalling is not present, MACF1 associates with a complex ... However, upon Wnt signaling, MACF1 is involved in a translation and binding of the axin complex to LTP6 at the cell membrane. ...
GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of the target gene N-Myc, an oncogenic driver of ... One commonly proposed mechanism suggests that glypicans behave as co-receptors which bind both the ligand and the receptor. ... GPC1 expression is well-above normal in human breast cancers, while expression of GPC1 is low in healthy breast tissue. ... GPC1 expression is severely high in pancreatic ductal adenocarcinoma cells, and results indicate that GPC1 expression is linked ...
The Wnt signaling pathways are a group of signal transduction pathways made of proteins that pass signals into a cell through cell surface receptors. Three Wnt signaling pathways have been characterized: the canonical Wnt pathway, the noncanonical planar cell polarity pathway, and the noncanonical Wnt/calcium pathway. All three pathways are activated by binding a Wnt-protein ligand to a Frizzled family receptor, which passes the biological signal to the Dishevelled protein inside the cell. The canonical Wnt pathway leads to regulation of gene transcription, and is thought to be negatively regulated in part by the SPATS1 gene. The noncanonical planar cell polarity pathway regulates the cytoskeleton that is responsible for the shape of the cell. The noncanonical ...
The Pax Genes, or Paired-Box Containing Genes, play important roles in the development and proliferation of multiple cell lines, development of organs, and development and organization of the central nervous system.[7] The transcription factor gene Pax2 is important in the regionalized embryological development of the central nervous system. In mammals, the brain is developed in three regions: the forebrain, midbrain, and the hindbrain.[8] Concentration gradients of fibroblast growth factor 8 (FGF8) and Wingless-Type MMTV Integration Site Family, Member 1 (Wnt1) control expression of Pax2 during development of the Mesencephalon, or midbrain.[9] Similar patterning during embryological development can be observed in "basal chordates or ascidians," in which organization of the central nervous system in ascidian larvae are also controlled by fibroblast growth factor genes.[8] The Pax2 gene encodes for the transcription factor which appears to be essential in the ...
Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. WIF1 is a lipid-binding protein that binds to Wnt proteins and prevents them from triggering signalling. WNT proteins are extracellular signaling molecules involved in the control of embryonic development. This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals. GRCh38: Ensembl release 89: ENSG00000156076 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000020218 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". "Entrez Gene: WIF1 WNT ...
Proto-oncogene protein Wnt-1 is a protein that in humans is encoded by the WNT1 gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading ...
... (LEF1) is a protein that in humans is encoded by the LEF1 gene. Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). LEF1 is highly overexpressed and associated with disease progression and poor prognosis in B-cell chronic lymphocytic leukemia. It is also a promising potential drug target. Lymphoid enhancer-binding factor 1 has been shown to interact with: ALX4, AML-1, CTNNB1, EP300, MITF PIAS4, SMAD2, and SMAD3. GRCh38: Ensembl release 89: ENSG00000138795 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000027985 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Milatovich A, Travis A, Grosschedl R, Francke U (Mar 1992). "Gene for lymphoid ...
... is a secreted protein that in humans is encoded by the Wnt4 gene, found on chromosome 1. It promotes female sex development and represses male sex development. Loss of function can have serious consequences, such as female to male sex reversal. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and embryogenesis. WNT4 is involved in a couple features of pregnancy as a downstream target of BMP2. For example, it regulates endometrial stromal cell proliferation, survival, and differentiation. These processes are all necessary for the development of an embryo. Ablation in female mice results in subfertility, with defects in implantation and decidualization. For instance, there is a decrease in responsiveness to progesterone signaling. ...
Kim B، Koo H، Yang S، Bang S، Jung Y، Kim Y، Kim J، Park J، Moon RT، Song K، Lee I (June 2006). "TC1(C8orf4) correlates with Wnt/beta-catenin target genes and aggressive biological behavior in gastric cancer". Clinical Cancer Research. 12 (11 Pt 1): 3541-8. PMID 16740781. doi:10.1158/1078-0432.CCR-05-2440. ...
Skrót Wnt powstał z połączenia nazw Wg (wingless) i Int[2]. Gen wingless został opisany u Drosophila melanogaster jako gen regulujący biegunowość segmentów ciała owada, regulującego proces segmentacji w embriogenezie[3], a także proces powstawania odnóży w trakcie przepoczwarzenia[4]. Geny INT zostały zidentyfikowane u kręgowców jako geny sąsiadujące z miejscami integracji genów mysiego wirusa raka sutka (mouse mammary tumor virus, MMTV)[5]. Int-1 i wingless zostały uznane za homologi na podstawie analizy sekwencji aminokwasowej kodowanych białek. Mutacje wingless u muszek powodowały powstanie bezskrzydłych mutantów, natomiast guzy wywołane przez MMTV zawierały liczne sekwencje genetyczne wirusa, powodujące nadekspresję genów rodziny Wnt. Kolejne badania wykazały, że Wnt należą do dużej grupy morfogenów wydzielanych, zawierającej ligandy o głębokim znaczeniu w powstawaniu planu ciała u wszystkich badanych ...
Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. GRCh38: Ensembl release 89: ENSG00000120057 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000018822 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Melkonyan HS, Chang WC, Shapiro JP, Mahadevappa M, Fitzpatrick PA, Kiefer MC, Tomei LD, Umansky SR (Jan 1998). "SARPs: A family of secreted apoptosis-related proteins". Proc Natl Acad Sci U S A. 94 (25): 13636-41. doi:10.1073/pnas.94.25.13636. PMC ...
Uvojiti-9 je protein koji je kod ljudi kodiran FZD9 genom.[1][2][3] FZD9 se takođe označava kao CD349 (klaster diferencijacije 349). Članovi familije „uvojitih" proteina su 7-transmembranski receptori za Wnt signalne proteine. FZD4 protein sadrži signalni peptid, cisteinom bogati domen u N-terminalnom ekstracelularnom regionu, 7 transmembranski domen, i C-terminalni PDZ vezujući motiv. FZD9 gen je lociran unutar regiona na hromozomu 7 čije brisanje proizvodi Vilijamsonov sindrom. Heterozigotno brisanje FZD9 gena može da doprinese fenotipu Vilijamsovog sindroma. FZD9 je predominantno izražen u mozgu, testisima, očima, skeletalnim mišićima, i bubrezima.[3] ...
Uvojiti-4 je protein koji je kod ljudi kodiran FZD4 genom.[1][2][3] FZD4 je takođe označen sa CD344 (klaster diferencijacije 344). Članovi familije „uvojitih" proteina su 7-transmembranski receptori za Wnt signalne proteine. FZD4 protein sadrži signalni peptid, cisteinom bogati domen u N-terminalnom ekstracelularnom regionu, 7 transmembranski domen, i C-terminalni PDZ vezujući motiv. Uvojiti-4 je jedini predstavnik family „frizzeled" receptora koji se potentno vezuje za ligand norin. Taj ligand je funkcionalno sličan sa strukturno različitim Wingless tipom proteina. FZD4 signalizacija indukovana norinom reguliše vaskularni razvoj retine kičmenjaka i kontroliše krvne sudove uva. Većina uvojitih receptora je spregnuta sa beta-kateninskim kanoničkim signalnim putem. Ovaj protein potencijalno učestvuje kao pozitivni regulator signalnog puta Wingless tipa MMTV mesta integracije.[3] ...
Uvojiti-7 je protein koji je kod ljudi kodiran FZD7 genom.[1][2][3] Članovi familije „uvojitih" proteina su 7-transmembranski receptori za Wnt signalne proteine. FZD7 protein sadrži signalni peptid, cisteinom bogati domen u N-terminalnom ekstracelularnom regionu, 7 transmembranski domen, i C-terminalni PDZ vezujući motiv. Izražavanje FZD7 gena može da umanji APC funkciju i pojača beta-katenin-posredovane signale u slabo diferenciranom ljudskim karcinomima jednjaka.[3] ...
Uvojiti-8 je protein koji je kod ljudi kodiran FZD8 genom.[1][2] Članovi familije „uvojitih" proteina su 7-transmembranski receptori za Wnt signalne proteine. FZD8 protein sadrži signalni peptid, cisteinom bogati domen u N-terminalnom ekstracelularnom regionu, 7 transmembranski domen, i C-terminalni PDZ vezujući motiv. Ovaj gen je visoko izražen u dva tipa ćelija ljudskog kancera. Kristalna struktura ekstracelularnog, cisteinom-bogatog domena, srodnog mišjeg proteina je određena.[2] ...
01/01/2001 - "Alterations of the expression of beta-catenin, and its binding partners E-cadherin and the adenomatous polyposis ... 05/01/2001 - "If certain polymorphisms in Wnt signaling through beta-catenin predispose to colon cancer, this might manifest as ... Non-Receptor Type 13 Protein Tyrosine Phosphatase 4. gamma Catenin (Plakoglobin) 5. WT1 Proteins ... 05/01/1999 - "Expression of E-cadherin, alpha, beta and gamma-catenin and p120ctn, and of the adenomatous polyposis coli ...
Wnt-1, Wnt-3a, and Wnt-7a have the greatest transforming capacity; Wnt-2, Wnt-5b, and Wnt-7b have an intermediate transforming ... tumor with retained expression (+++) of Wnt-5a, Syk, and DDR1; lower micrographs, tumor with reduced expression of Wnt-5a (−), ... we noted a covariation between decreased expression of Wnt-5a and Syk (Table 2). In contrast to the expression of Wnt-5a, none ... Expression and Signaling Activity of Wnt-5a/Discoidin Domain Receptor-1 and Syk Plays Distinct but Decisive Roles in Breast ...
Wnt signalling in osteoblasts regulates expression of the receptor activator of NFkappaB ligand and inhibits osteoclastogenesis ... we determined expression of a second Wnt co-receptor LRP6 in human osteoblasts, CD14(+) osteoclast progenitors and mature ... Activation of Wnt signalling downregulated RANKL mRNA and protein expression, and overexpression of fulllength beta-catenin, ... Reports implicating Wnt signalling in the regulation of bone mass have prompted widespread interest in the use of Wnt mimetics ...
The four subfamilies of Fz receptors also evolved early in metazoan evolution. To date, Fz receptors have been characterised ... It has been shown that Wnt ligands represent an ancient and diverse family of proteins that likely function in complex ... The four subfamilies of Fz receptors also evolved early in metazoan evolution. To date, Fz receptors have been characterised ... It has been shown that Wnt ligands represent an ancient and diverse family of proteins that likely function in complex ...
1. Over-expression of the UNC-6/Netrin receptor UNC-5 causes DTC phase 3 A/P polarity reversals. DIC images of posterior gonad ... The Wnt Frizzled Receptor MOM-5 Regulates the UNC-5 Netrin Receptor through Small GTPase-Dependent Signaling to Determine the ... The Wnt Frizzled Receptor MOM-5 Regulates the UNC-5 Netrin Receptor through Small GTPase-Dependent Signaling to Determine the ... Here, we reveal a regulatory link between the Wnt Frizzled receptor, MOM-5, and the Netrin receptor UNC-5. We present evidence ...
Here, we identify the Wnt receptor Frizzled (Fzd) as a key target of the USP6 oncogene. Increased expression of USP6 increases ... USP6 regulates WNT signaling at the level of the Wnt ligand and receptor complex. (A) Inhibition of USP6 induced Wnt signaling ... Regulation of Wnt target gene expression by USP6. (A) USP6 activates Wnt signaling in HeLa cells: HeLa cells were transfected ... The Wnt signaling pathways play pivotal roles in carcinogenesis. Modulation of the cell-surface abundance of Wnt receptors is ...
positive regulation of gene expression Source: MGI. *positive regulation of insulin-like growth factor receptor signaling ... positive regulation of canonical Wnt signaling pathway Source: MGI. * ... help/expression_section target=_top>More...,/a>,/p>Expressioni. ,p>This subsection of the Expression section provides ... Gene expression databases. Bgee dataBase for Gene Expression Evolution. More...Bgeei. ENSMUSG00000061048 Expressed in 180 organ ...
COX-2 induces breast Cancer stem cells via EP4/PI3K/AKT/NOTCH/WNT Axis. Stem cells (Dayton, Ohio). 2016;Google Scholar ... EP3 receptor was expressed in 205 of 289 samples analyzed (70.9%). EP3 receptor expression was not associated with ... Patient data regarding patient age, hormone receptor status (estrogen receptor [ER], progesterone receptor [PR]), HER2- ... EP1-4 receptor expression has been shown in a variety of cancers, including breast cancer [13, 14, 15]. Exploiting the role of ...
Total protein expression in the lysate was confirmed by IB of the input. Transferrin Receptor (TfR) was used as a control ... due to increased Wnt signaling. Bighead protein bound specifically to the Wnt coreceptor lipoprotein receptor-related protein 6 ... Bighead mRNA expanded the expression of a number of head markers, blocked expression of the En2 Wnt target gene, prevented ... Further supporting the possibility that Bighead encodes a Wnt inhibitor, expression of the zygotic Wnt target gene Engrailed 2 ...
... such as the Epidermal Growth Factor Receptor. Downstream of the receptors, this pathway involves the activation of a kinase ... such as fibroblast growth factor receptor substrate 2, flotillin-1 and mitogen-activated protein kinase organizer 1. ... pathway is the canonical signaling pathway for many receptor tyrosine kinases, ... Vascular Endothelial Growth Factor Receptor Family in Ascidians, Halocynthia roretzi (Sea Squirt). Its High Expression in ...
Dopamine D2 receptor modulates Wnt expression and control of cell proliferation *Fei Han ... Rights & permissionsfor article Dopamine D,sub,2,/sub, receptor modulates Wnt expression and control of cell proliferation . ... receptors in pancreatic beta cell insulin secretion . Opens in a new window. ... New roles for dopamine D2 and D3 receptors in pancreatic beta cell insulin secretion *Zachary J. Farino ...
MEHP also repressed the expression of AR and inactivated WNT signaling, and then led to the commitment of cells to apoptosis ... The loss of the frizzed receptor 7 and the gain of p21CIP were responsible for the stimulatory effect of MEHP on AR-mediated ... Although the androgen receptor (AR) has been implicated in the promotion of apoptosis in testicular cells (TSCs), the molecular ... were supplemented with leukemia inhibitory factor and bone morphogenetic protein 4 to maintain and stabilize the expression of ...
Wnt is a secreted protein that binds to receptors of the Frizzled family. This interaction activates a signaling cascade that ... and inducing the expression of Wnt-target genes. In Caenorhabditis elegans, inhibition of CKI produced the same mesoderm ... Peters, J.M., McKay, R.M., McKay, J.P., and Graff, J.M. (1999) Casein kinase I transduces Wnt signals. Nature 401: 345-350. ... reveal that in the developing frog and worm, a serine-threonine kinase called casein kinase I (CKI) functions in the Wnt ...
... is activated and translocates to the nucleus when Wnt proteins bind to the frizzled (Fz) receptors in a paracrine pattern [30 ... Consistently, sh-GPC3 significantly reduced GPC3 protein expression, while vector-GPC3 enhanced GPC3 expression (Figure 2B). In ... The relative expression of mRNA was calculated by the 2−ΔΔCt method. Primers used in the present study were provided by Beijing ... A,B) RT-PCR and WB to analyse the mRNA and protein expression of Wnt1, β-catenin, PI3K, AKT and STAT1 in LTEP-s. (C) ...
... which WNT-5B induced TGF-β downstream signalling suggests that WNT-5B increases the bioavailability of TGF-β for its receptor, ... reduced expression of WNT (target) genes, e.g. WNT-10B, WNT-2, FZD1 and axin1/2, was observed [37]. Here, WNT/β-catenin ... WNT-5A, WNT-5B and WNT-7B mRNA, and that cigarette smoke exposure specifically upregulates WNT-5B mRNA expression in vitro [11 ... without differential expression of WNT-5B [11]. Again, we did not observe significant differences in baseline expression of WNT ...
Canonical Wnt/betacatenin regulation of liver receptor homolog-1 mediates pluripotency gene expression. Stem Cells. 2010;28: ... Zhao G, Zhou X, Fang T, Hou Y, Hu Y. Hyaluronic acid promotes the expression of progesterone receptor membrane component 1 via ... Alterations in the expression, structure and function of progesterone receptor membrane component-1 (PGRMC1) in premature ... Peluso JJ, Pappalardo A, Losel R, Wehling M. Progesterone membrane receptor component 1 expression in the immature rat ovary ...
Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent ... Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. ... LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal.. J Clin Endocrinol Metab ... Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May ...
Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent ... Expression of LEF1 in mantle cell lymphoma.. Ann Diagn Pathol 26:57-59 (2017). IHC-P ; Human . Read more (PubMed: 28038713) » ... Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May ... Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. ...
Song, S., Shackel, N., Wang, X., Ajami, K., McCaughan, G., Gorrell, M. (2011). Discoidin Domain Receptor 1: Isoform expression ... Identification of increased WNT pathway gene expression in biliary liver disease 3. Identification of the collagen receptor ... Song, S., Shackel, N., Wang, X., Ajami, K., McCaughan, G., Gorrell, M. (2011). Discoidin Domain Receptor 1: Isoform expression ... Analysis of gene expression in peripheral blood mononuclear cells (PBMC) in chronic hepatitis C (HCV) Infection; Shackel N; ...
The data described above suggest that Nodal signalling directly controls the expression of non-Wnt/PCP movement-effector genes ... the non-canonical Wnt pathway is triggered by Wnt11, which signals through Frizzled receptors to recruit Dishevelled (Dsh) at ... Nodal signalling does not control gastrulation via the Wnt/PCP pathway. Non-canonical Wnt/PCP signalling is a prominent ... which bind to Nodal ligands and the EGF-CFC co-receptor, thereby preventing the formation of receptor complexes (Shen, 2007). ...
Vitamin D-induced vitamin D receptor expression induces tamoxifen sensitivity in MCF-7 stem cells via suppression of Wnt/β- ... Vitamin D-induced vitamin D receptor expression induces tamoxifen sensitivity in MCF-7 stem cells via suppression of Wnt/β- ... Vitamin D-induced vitamin D receptor expression induces tamoxifen sensitivity in MCF-7 stem cells via suppression of Wnt/β- ... Vitamin D-induced vitamin D receptor expression induces tamoxifen sensitivity in MCF-7 stem cells via suppression of Wnt/β- ...
... receptor EP4. Paricalcitol was injected into IR-exposed HK-2 cells and mice subjected to bilateral kidney ischemia for 23 min ... Y. Araki, S. Okamura, S. P. Hussain et al., "Regulation of cyclooxygenase-2 expression by the WNT and ras pathways," Cancer ... Paricalcitol Upregulates Cellular Membrane Expression of EP4 in HK-2 Cells. We examined the induction of PGE2 receptor subtypes ... IR injury increased expression of the proapoptotic protein Bax and decreased the expression of the antiapoptotic protein Bcl-2 ...
Tamai, LDL-receptor-related proteins in Wnt signal transduction. 2000, Pubmed, Xenbase Tannahill, Developmental expression of ... Hoppler, Wnt signalling: variety at the core. 2007, Pubmed Hoppler, Expression of a dominant-negative Wnt blocks induction of ... N) Temporal expression of Lrig3 during Xenopus development. (O-R) Expression of lrig3 in zebrafish. Transcripts are present ... Saint-Jeannet, Regulation of dorsal fate in the neuraxis by Wnt-1 and Wnt-3a. 1998, Pubmed, Xenbase Sato, Neural crest ...
Human dopamine D1-D5 receptors (D1R-D5R) in pCMV5 were described previously (Cho et al., 2006). Receptor expression levels of ... Effects of dopamine receptor subtypes on the Wnt signaling. A, effects of dopamine receptor subtypes on the Wnt signaling were ... Wnt. Wingless/int. GRK. G protein-coupled receptor kinase. LRP. lipoprotein receptor-related protein. GSK. glycogen synthase ... To determine the receptor regions of D2R involved in the regulation of Wnt signaling, chimeric receptors between D2R and D3R in ...
mRNA expression analyses of Wnt ligands, Fzd receptors and Wnt antagonists. RT-PCR detection of mRNAs for Wnt ligands, Fzd ... Fzd receptors and Wnt antagonists in human BM B progenitor cells and regulated expression of Fzd receptors and co-receptors, β- ... The relative mRNA expression levels of Wnt receptors and co-receptors, β-catenin, plakoglobin, LEF-1 and TCF-4 were quantified ... mRNA expression of Wnt ligands 1-19, Fzd receptors 1-10, Wnt antagonists sFRP1-4, WIF1, Dkk1 and Dkk4 ...
  • The tyrosine Y2502.39 in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled. (muni.cz)
  • Abstract 5643: Role of leptin-induced Notch-Wnt crosstalk in proliferation/survival of triple negative breast cancer cells. (aacrjournals.org)
  • We present evidence showing that MOM-5/Frizzled signals through small GTPases to negatively regulate the UNC-5 Netrin receptor. (prolekare.cz)
  • PGE2 signals via EP receptors 1-4, whose functions are analyzed in current research in search for targeted anti-PG therapies. (springer.com)
  • J:169706 Abdul-Ghani M, Dufort D, Stiles R, De Repentigny Y, Kothary R, Megeney LA, Wnt11 promotes cardiomyocyte development by caspase-mediated suppression of canonical Wnt signals. (jax.org)
  • This review explores intriguing connections between Wnt and other growth factor signals, β-catenin distribution, and cadherin-mediated cell adhesion ( Fig. 1 , inset). (sciencemag.org)
  • The Fz-LRP receptor complex probably accomplishes this by generating multiple signals in the cytoplasm. (biologists.org)
  • Commonly used molecular markers for EMT include increased expression of N-cadherin and vimentin, nuclear localization of β-catenin, and increased production of the transcription factors such as Snail1 (Snail), Snail2 (Slug), Twist, EF1/ZEB1, SIP1/ZEB2, and/or E47 that inhibit E-cadherin production. (rupress.org)
  • Bighead joins two other Wnt antagonists, Dkk1 and Angptl4, which function as Lrp6 endocytosis regulators. (pnas.org)
  • This effect was blocked by the Wnt antagonists sFRP1 or Dkk1. (biomedsearch.com)
  • J:95872 De Langhe SP, Sala FG, Del Moral PM, Fairbanks TJ, Yamada KM, Warburton D, Burns RC, Bellusci S, Dickkopf-1 (DKK1) reveals that fibronectin is a major target of Wnt signaling in branching morphogenesis of the mouse embryonic lung. (jax.org)
  • In tumors from breast cancer patients, the protein expression of Wnt-5a and Syk were differently regulated at the translational and transcriptional level, respectively. (aacrjournals.org)
  • We analysed WNT-5B protein expression in the lung tissue of COPD patients and (non)smoking controls, and investigated the effects of cigarette smoke exposure on WNT-5B expression in COPD and control-derived primary bronchial epithelial cells (PBECs). (ersjournals.com)
  • It has been reported recently that transnasal administration of siRNA is able to down-regulate protein expression ( 9 ) as well as the respiratory virus replication ( 10 ) specifically in the lungs. (jimmunol.org)
  • In addition, ß-catenin, Cyclin D1, and c-Myc protein expression was inhibited by ethanol exposure at P3, but not P10 or P20. (omicsonline.org)
  • However, Wnt-5a expression has opposite effects in breast cancer and melanoma cells. (aacrjournals.org)
  • In breast epithelial cells that are normally firmly adherent, a decreased expression of Wnt-5a and thus a decreased adhesion will increase their motility, whereas, in the more loosely adherent cells, an elevated expression of Wnt-5a and increased adhesion will increase their motility and thus the metastatic potential of these tumor cells. (aacrjournals.org)
  • Recently, we found DDR1 to be an important target of noncanonical Wnt-5a signaling in human mammary cells (6) . (aacrjournals.org)
  • These cells are born post-embryonically in the ventral mid-body and migrate along a stereotyped path involving 3 stages of polarized movements along A/P and D/V axes. (prolekare.cz)
  • Although the androgen receptor (AR) has been implicated in the promotion of apoptosis in testicular cells (TSCs), the molecular pathway underlying AR-mediated apoptosis and its sensitivity to environmental hormones in TSCs and induced pluripotent stem cells (iPSCs) remain unclear. (mdpi.com)
  • Immunohistochemistry, Western blot (WB) and real-time PCR (RT-PCR) assays were used to determine the expression patterns of GPC3 in lung SCC tissues and cells. (portlandpress.com)
  • The results showed that GPC3 expression levels in lung SCC tissues and cells were significantly elevated, and the high expression of GPC3 significantly promoted cell growth and tumorigenesis and repressed cell apoptosis, as well as increased β-catenin expression. (portlandpress.com)
  • Zhao G, Zhou X, Fang T, Hou Y, Hu Y. Hyaluronic acid promotes the expression of progesterone receptor membrane component 1 via epigenetic silencing of miR-139-5p in granulosa cells. (springer.com)
  • MCF-7 cells were treated with 1,25(OH) 2 D 3 and their levels of VDR expression, viability, and apoptosis were detected. (bioscirep.org)
  • Levels of VDR expression and Wnt/β-catenin signaling in CD133 + cells were markedly lower and higher than those in CD133 − cells, respectively. (bioscirep.org)
  • Vitamin D-induced VDR expression increased the sensitivity of MCF-7 stem cells to tamoxifen by inhibiting Wnt/β-catenin signaling. (bioscirep.org)
  • Lrig3 could attenuate Fgf signaling in animal caps, did interact with Fgf receptor 1 in cultured cells and, according to context, decreased or increased the induction of NC markers by Fgf. (xenbase.org)
  • J:40358 Austin TW, Solar GP, Ziegler FC, Liem L, Matthews W, A role for the Wnt gene family in hematopoiesis: expansion of multilineage progenitor cells. (jax.org)
  • Epithelial cells move as a sheet en block, whereas mesenchymal migration is considerably more dynamic. (rupress.org)
  • Objective/Methods: The effects of leptin-Notch-Wnt crosstalk on the proliferation and survival (MTT-Vybrant assay and Annexin V-flow cytometry) of breast cancer cells responsive to estrogen (MCF-7 and T47D) and TNBC (MDA-MB231 and HC1806) were investigated. (aacrjournals.org)
  • In a series of experiments the cells were treated with chemotherapeutic drugs, doxorubicin and cisplatin plus leptin and the activation of caspase 3, levels of Bcl-2 and, the drug resistance marker (multidrug resistance-associated protein 1, ABCC1/MRP1) were determined using as control the etoposide resistant MCF-7/VP cell line. (aacrjournals.org)
  • Ubiquitination of β-catenin is greatly reduced in Wnt-expressing cells. (oxfordreference.com)
  • ROR1, a receptor in the noncanonical Wnt/planar cell polarity (PCP) pathway, is upregulated in malignant B cells of chronic lymphocytic leukemia (CLL) patients. (aacrjournals.org)
  • It has been shown that the Wnt/PCP pathway drives pathogenesis of CLL, but which factors activate the ROR1 and PCP pathway in CLL cells remains unclear. (aacrjournals.org)
  • 2,3 Although vascular calcification was once considered only a passive process of dead and dying cells, work from laboratories worldwide has now highlighted that arterial biomineralization is an actively regulated form of calcified tissue metabolism. (ahajournals.org)
  • Control cells differentiated into adipocytes, as assessed by Oil Red-O staining and by immunoblot detection of the adipocyte fatty acid binding protein, 422/aP2 ( 12 ), whereas cells expressing Wnt-1 failed to differentiate ( Fig. 1 A). Neither lipid droplets nor 422/aP2 were detected in Wnt-1-expressing cells ( 12 ). (sciencemag.org)
  • Sun et al ( 22 ) analyzed different expression patterns of stem-cell markers in HBV-associated cirrhotic livers and in HCC and demonstrated that the stem-like cells possessed tumorigenic capacity and that these cells might be LCSCs. (spandidos-publications.com)
  • Moreover, we analyzed primary ATII cells from mice with bleomycin-induced lung injury, a model exhibiting activated Wnt/β-catenin signaling in vivo . (biologists.org)
  • Coculture with endometrial cells from the menstrual phase confirmed an increase in expression of active β-catenin in the eMSCs. (springer.com)
  • Anti-R-spondin-1 antibody reduced the stimulatory action of endometrial niche cells on WNT/β-catenin activation in the T cell factor/lymphoid enhancer-binding factor luciferase reporter assay. (springer.com)
  • Moreover, the mRNA level and protein immunoreactivities of leucine-rich repeat-containing G-protein coupled receptor 5 were higher in eMSCs than unfractionated stromal cells. (springer.com)
  • Our findings indicate a role of WNT/β-catenin signalling in regulating activities of endometrial stem/progenitor cells during menstruation. (springer.com)
  • The identification of endometrial stem/progenitor cells in a tissue with high turnover rate suggests the presence of a well-orchestrated regulatory network controlling stem cells fate [ 3 ]. (springer.com)
  • From there it can be exported to the general circulation, where it is combined with a transport protein, RBP (retinol binding protein), that facilitates its uptake by cells through the receptor STRA6 (stimulated by retinoic acid gene 6). (els.net)
  • GM‐CSF (Granulocyte‐macrophage colony‐stimulating factor), a key factor in this process, is produced notably by stromal cells and type 3 innate lymphoid cells (ILC3). (els.net)
  • The progenitor preserves its WNT signaling identity and maintains WNT signaling with underlying mesenchymal neighbors, while its overlying epithelial cells become WNT-restricted. (elifesciences.org)
  • During embryonic development, the first of three spatially positioned arrays of hair placodes emerges when some cells within an epithelial monolayer begin to experience a higher level of WNT signal than their neighbors ( DasGupta and Fuchs, 1999 ). (elifesciences.org)
  • Histologically, siRNA against DDR1 successfully reduced in vivo DDR1 expression and attenuated bleomycin-induced infiltration of inflammatory cells. (jimmunol.org)
  • DDR1 is constitutively expressed in the normal tissues of organs, such as the lungs, kidneys, colon, and brain, as well as in the tumor cells of epithelial origin, such as those of mammary, ovarian, and lung carcinomas ( 3 ). (jimmunol.org)
  • As in planarians, muscle cells are shown to be a source of secreted Wnt ligands, providing an explanation for the retention of a muscle layer in the immotile E. multilocularis larva. (biomedcentral.com)
  • SW403 cells showed a dose-dependently decreased expression of 5-HTR1D, meanwhile, β-catenin level was significantly decreased in nucleus of cells cultured with GR127935. (biomedcentral.com)
  • PFOA (10-100μM) significantly reduced Jeg-3 spheroid attachment on RL95-2 endometrial cells. (hku.hk)
  • PFOA also suppressed β-catenin expression in Jeg-3 cells. (hku.hk)
  • The Wnt agonist Wnt3a stimulated β-catenin expression in Jeg-3 cells and reversed the PFOA suppression of the spheroid attachment. (hku.hk)
  • D ) TRIB2 expression in HEK293T cells, Huh7, Hep3B and HepG2 cells. (nih.gov)
  • E ) Immunoblot analysis of TRIB2 expression in control and DN-TCF4-expressing HepG2 cells. (nih.gov)
  • Immunoblot analysis of TRIB2 in HL7702 (F) and Huh7 (G) cells with or without expression of DA-βCatenin. (nih.gov)
  • Immunofluorecence staining of βCatenin and TRIB2 in Huh7 cells with or without Wnt1 over-expression (H). See also Figure S1, S2, S3 and Table S1, S2, S3. (nih.gov)
  • CXCR4 belongs to the superfamily of the seven transmembrane domain heterotrimeric G protein-coupled receptors and is functionally expressed on the cell surface of various types of cancer cells. (dovepress.com)
  • Here, we used high-throughput sequencing of differentiating ectodermal explants to isolate yet another potent Wnt inhibitor expressed in Spemann organizer tissue. (pnas.org)
  • Treatment with IWP-2, a WNT inhibitor, suppressed the observed effects. (springer.com)
  • They signal across the plasma membrane by interacting with serpentine receptors of the Frizzled (Fz) family and members of the low-density-lipoprotein-related protein (LRP) family. (biologists.org)
  • Wnt/β-catenin signaling involves at least two types of receptors: Frizzled (Fz) and low-density-lipoprotein-related protein (LRP). (biologists.org)
  • 10 Furthermore, we highlight differences between the low-density lipoprotein receptor (LDLR)-deficient and apolipoprotein E (apoE)-deficient murine models ( Table 2 ) that help articulate the multifaceted contributions of dyslipidemia, diabetes mellitus, and uremia to arterial calcium deposition. (ahajournals.org)
  • In this study, we show that among five different dopamine receptor subtypes, D 2 receptor (D 2 R) selectively inhibited the Wnt signaling, which was measured by lymphoid enhancing factor-1 (LEF-1)-dependent transcriptional activities. (aspetjournals.org)
  • In addition, EBV is the old but current standard of Morimoto (13/28 able to induce neoangiogenesis, but we found only minor capillary solid, 7/28 venous, 5/28 cavernous, 3/28 changes on the transcriptional level, including increased mixed capillary-cavernous). (deepdyve.com)
  • We hypothesized that estrogen receptor-α (ER-α) status in endometrial carcinomas, associated with poor prognosis, is reflected in transcriptional signatures suggesting targets for new therapy. (nih.gov)
  • In cultured neurons, we found that the HIV coat protein gp120 increased the transcriptional expression of BACE1 through repression of PPAR? (jove.com)
  • The expression of NOS2 is regulated by inflammatory cytokines predominantly at the transcriptional level ( 9 - 14 ). (aacrjournals.org)
  • In BPD, PPARγ has been shown to be downregulated in several animal models, while the canonical WNT/β-catenin pathway has been upregulated. (frontiersin.org)
  • PPARγ is upregulated in some diseases, while the WNT/β-catenin system is downregulated ( 5 ). (frontiersin.org)
  • In the present study, we review the respective roles in BPD of both the canonical WNT pathway and PPARγ, which are determined by TGF-β signaling, and discuss their possible implications for the therapeutic treatment of BPD. (frontiersin.org)
  • RA can also be delivered to PPARβ/γ receptors by FABP5 (fatty‐acid binding protein 5). (els.net)
  • Differential expression of microRNA species in human gastric cancer versus non-tumorous tissues. (springer.com)
  • Moreover, the delivery of a modified lin28 siRNA or a let-7a agomir into the premalignant mammary tissues of MMTV- wnt-1 mice resulted in a complete rescue of the stem cell phenotype driven by Wnt/β-catenin pathway. (biologists.org)
  • Under obese condition, a decrease in FAM3A expression in adipose tissues plays important roles in the development of adipose dysfunction and type 2 diabetes. (antibodies-online.com)
  • Thus studies of Wnt signaling have opened up new avenues in biomedical research, including molecular embryology, stem cell biology, tumorigenesis, regenerative medicine, and rational drug discovery. (ahajournals.org)
  • 3 Department of Developmental Biology, Beckman Center for Molecular and Genetic Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. (sciencemag.org)
  • Here we show that Wnt signaling, likely mediated by Wnt-10b, is a molecular switch that governs adipogenesis. (sciencemag.org)
  • The signaling interactions between Wnt-5a/DDR1 and Syk were addressed in mammary cell lines. (aacrjournals.org)
  • The expression of Wnt-5a and Syk correlated in four of five tumor cell lines. (aacrjournals.org)
  • As a cell surface proteoglycan anchored by glycosyl-phosphatidylinositol, Glypican-3 (GPC3) is reported to be highly expressed in hepatocellular carcinoma (HCC) and to promote cell proliferation and tumorigenesis through activating Wnt/β-catenin signalling. (portlandpress.com)
  • Expression of microRNAs in basal cell carcinoma. (springer.com)
  • Liu S, Xie L, Qi B, Ma C, Sang L, Li H. Differential expression profiles of microRNAs/mRNA and docking study in oral squamous cell carcinoma. (springer.com)
  • Interestingly, however, Nodal does not appear to mobilise the Wnt/PCP pathway, which is known to control cell and tissue polarity. (biologists.org)
  • CONCLUSION: These results indicate that canonical Wnt signaling is involved in human BM B lymphopoiesis where it acts as a negative regulator of cell proliferation in a direct or stroma dependent manner. (biomedsearch.com)
  • We are first to report that in PTSMTs, a non-canonical activation of WNT, independent of beta-catenin, drives tumor cell proliferation via MTOR/AKT1, MYC and Cyclin D2. (deepdyve.com)
  • HIV-SMT) or congenital immune defects (CI- cell of origin is derived from a perivascular non-endothe- SMT) [1, 3, (deepdyve.com)
  • enforced expression of let-7 blocks the Wnt/β-catenin pathway-stimulated breast cancer stem cell phenotype. (biologists.org)
  • Thus, Wnt/β-catenin pathway, Lin28, and let-7 miRNAs, three of the most crucial stem cell regulators, connect in one signal cascade. (biologists.org)
  • The central player is β-catenin, which is a transcription cofactor with T cell factor/lymphoid enhancer factor TCF/LEF in the Wnt pathway ( 2 ) and a structural adaptor protein linking cadherins to the actin cytoskeleton in cell-cell adhesion ( 5 ). (sciencemag.org)
  • It has become increasingly evident in recent years that, apart from the key role that thrombin plays in the blood coagulation cascade, thrombin also elicits cellular actions via the activation of proteinase-activated receptors, which are present in many cell types. (powells.com)
  • Receptor dynamics and cell signaling: enzyme versus peptide-mediated activation 2.4.1. (powells.com)
  • Cell type specific expression of thrombin receptors 3.3. (powells.com)
  • Cell type specific thrombin receptor signaling 3.4. (powells.com)
  • These results indicate that Sox17 suppresses cyclin D1 expression and cell proliferation by directly antagonizing β-catenin, whose activity in OPCs is stimulated not only by Wnt3a, but also by PDGF. (jneurosci.org)
  • Results: Leptin induced significant increase of TNBC cell proliferation, cyclin D1, bcl-2, ABCC1 and Notch/Wnt axis. (aacrjournals.org)
  • The connections of Wnt pathway components with cell cycle and centrosome: side effects or a hidden logic? (muni.cz)
  • Glypican-3 (GPC3) is a cell surface proteoglycan anchored by glycosyl-phosphatidylinositol, which was first discovered in patients with Simpson-Golabi-Behmel syndrome [ 8 ]. (bioscirep.org)
  • Thus, vitamin D and/or canonical Wnt pathway activation markedly reduced mineralization, with additive inhibitory effects on viable cell number. (garvan.org.au)
  • Importantly, the inhibitory effect of LiCl in this committed osteoblastic cell line contrasts with the stimulatory effects of genetic Wnt pathway activation in human and mouse bone tissue. (garvan.org.au)
  • In the cell, RA can enter the nucleus to activate transcription via ligation with the RARs (retinoic acid receptors). (els.net)
  • Beta-catenin is a multifunctional protein with critical roles in cell-cell adhesion, Wnt-signaling and the centrosome cycle. (stanford.edu)
  • Whereas the roles of β-catenin in cell-cell adhesion and Wnt-signaling have been studied extensively, the mechanism(s) involving β-catenin in centrosome functions are poorly understood. (stanford.edu)
  • We show that Wnt-TRIB2 activation is critical for cancer cell survival and transformation. (nih.gov)
  • More importantly, they were resistant to cisplatin, oxaliplatin and cyclophosphamide exhibiting high cross-resistance along with alterations in expression of cancer-stem cell markers such as CD133, CD166, CD24, CD26, CXCR4, CD271 and CD274. (biomedcentral.com)
  • Isolation of CSC from colon carcinomas can be accomplished by selection of subpopulation based on expression of one or multiple cell surface markers, i.e. (biomedcentral.com)
  • 4 Conversely, rather than inhibiting neurodegenerative diseases via an immunological pathway, the CB1 receptor suppresses cell death through protecting against excitotoxicity, overstimulation of excitatory receptors and simultaneous calcium release. (drmeletis.com)
  • 2 , 3 Chemokine receptors are a family of G protein-coupled cell surface receptors (GPCRs) with seven transmembrane-spanning domains. (dovepress.com)
  • The absence of a decrease in the WNT/β-catenin signaling during the canalicular stage of pulmonary development, partly related to inflammatory processes, a hallmark of BPD, will seriously affect normal development in the subsequent saccular and alveolar stages ( 7 ). (frontiersin.org)
  • The insert displays possible levels of interactions between Wnt signaling and cadherin-mediated adhesion (dotted lines) and the central role of β-catenin in both processes that are the focus of the review. (sciencemag.org)
  • In addition to its central role in blood coagulation, it has become increasingly apparent that thrombin and thrombin receptors are involved in many other physiological processes and can contribute to a variety of disease states such as tumor progression and metastasis, inflammation, neurological disorders and cardiovascular complications. (powells.com)
  • Thrombin and thrombin receptors are involved in many physiological processes and can contribute to a variety of disease states. (powells.com)
  • Lack of estrogen receptor-α is associated with epithelial-mesenchymal transition and PI3K alterations in endometrial carcinoma. (nih.gov)
  • In mice, endometrial LIF secretion can be induced by nidatory estrogen at day 4 of pregnancy [ 10 ] while exogenous estrogen and progesterone administration to ovariectomised mice were able to increase gp130 expression in the uterine glands [ 11 ]. (hindawi.com)
  • In hamsters, LIF secretion was induced by estrogen while the expression of LIFR and gp130 was induced by progesterone [ 12 ]. (hindawi.com)
  • The hypothesis that TLE3 expression was associated with response to taxane therapy was subsequently tested by conducting a retrospective validation study in a triple negative (estrogen receptor, progesterone receptor, and ERBB2 negative), uniformly high-grade breast cancer cohort. (aacrjournals.org)
  • She moved to Jan-Åke Gustafsson's lab at Karolinska Institutet in 2005, where she focused on the role of estrogen receptor beta in breast cancer. (ki.se)