A species of gram-negative, aerobic bacteria that is the causative agent of LEGIONNAIRES' DISEASE. It has been isolated from numerous environmental sites as well as from human lung tissue, respiratory secretions, and blood.
Gram-negative aerobic rods, isolated from surface water or thermally polluted lakes or streams. Member are pathogenic for man. Legionella pneumophila is the causative agent for LEGIONNAIRES' DISEASE.
An acute, sometimes fatal, pneumonia-like bacterial infection characterized by high fever, malaise, muscle aches, respiratory disorders and headache. It is named for an outbreak at the 1976 Philadelphia convention of the American Legion.
Infections with bacteria of the genus LEGIONELLA.
A species of free-living soil amoebae in the family Acanthamoebidae. It can cause ENCEPHALITIS and KERATITIS in humans.
A genus of free-living amoebae found in fresh water. The cysts usually pass harmlessly through the intestinal tract of man and may thus be found in feces. Occasionally, these organisms cause respiratory tract infections or generalized fatal meningoencephalitis.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
Means or process of supplying water (as for a community) usually including reservoirs, tunnels, and pipelines and often the watershed from which the water is ultimately drawn. (Webster, 3d ed)
A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract.
A branch of engineering concerned with the design, construction, and maintenance of environmental facilities conducive to public health, such as water supply and waste disposal.
The maintenance of certain aspects of the environment within a defined space to facilitate the function of that space; aspects controlled include air temperature and motion, radiant heat level, moisture, and concentration of pollutants such as dust, microorganisms, and gases. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Proteins found in any species of bacterium.
A genetically related subfamily of RAB GTP-BINDING PROTEINS involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS and through early Golgi compartments. This enzyme was formerly listed as EC 3.6.1.47.
A family of gram-negative, aerobic bacteria that do not form endospores or microcysts.
A genus of ameboid protozoa. Characteristics include a vesicular nucleus and the formation of several lodopodia, one of which is dominant at a given time. Reproduction occurs asexually by binary fission.
Members of a family of highly conserved proteins which are all cis-trans peptidyl-prolyl isomerases (PEPTIDYLPROLYL ISOMERASE). They bind the immunosuppressant drugs CYCLOSPORINE; TACROLIMUS and SIROLIMUS. They possess rotamase activity, which is inhibited by the immunosuppressant drugs that bind to them.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
An inhibitor of apoptosis protein that was initially identified during analysis of CHROMOSOME DELETIONS associated with SPINAL MUSCULAR ATROPHY. Naip contains a nucleotide binding oligomerization domain and a carboxy-terminal LEUCINE rich repeat.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Membrane-bound cytoplasmic vesicles formed by invagination of phagocytized material. They fuse with lysosomes to form phagolysosomes in which the hydrolytic enzymes of the lysosome digest the phagocytized material.
The immersion or washing of the body or any of its parts in water or other medium for cleansing or medical treatment. It includes bathing for personal hygiene as well as for medical purposes with the addition of therapeutic agents, such as alkalines, antiseptics, oil, etc.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Water containing no significant amounts of salts, such as water from RIVERS and LAKES.
A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.
Contamination of bodies of water (such as LAKES; RIVERS; SEAS; and GROUNDWATER.)
An order of ameboid protozoa that is commonly uninucleate and possess mitochondria. Most organisms are nonpathogenic.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
A species of gram-negative, aerobic bacteria in the genus LEGIONELLA, first isolated in Long Beach, California. Infection is associated with the use of contaminated potting SOIL.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Substances elaborated by bacteria that have antigenic activity.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
The functional hereditary units of BACTERIA.
An amorphous form of carbon prepared from the incomplete combustion of animal or vegetable matter, e.g., wood. The activated form of charcoal is used in the treatment of poisoning. (Grant & Hackh's Chemical Dictionary, 5th ed)
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
Hospital department whose primary function is the upkeep and supervision of the buildings and grounds and the maintenance of hospital physical plant and equipment which requires engineering expertise.
A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.
A greenish-yellow, diatomic gas that is a member of the halogen family of elements. It has the atomic symbol Cl, atomic number 17, and atomic weight 70.906. It is a powerful irritant that can cause fatal pulmonary edema. Chlorine is used in manufacturing, as a reagent in synthetic chemistry, for water purification, and in the production of chlorinated lime, which is used in fabric bleaching.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Techniques used in studying bacteria.
Inflammation of the lung parenchyma that is caused by bacterial infections.
Solitary or multiple collections of PUS within the lung parenchyma as a result of infection by bacteria, protozoa, or other agents.
Any of several processes in which undesirable impurities in water are removed or neutralized; for example, chlorination, filtration, primary treatment, ion exchange, and distillation. It includes treatment of WASTE WATER to provide potable and hygienic water in a controlled or closed environment as well as provision of public drinking water supplies.
Fluids composed mainly of water found within the body.
A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
An enzyme that catalyzes the isomerization of proline residues within proteins. EC 5.2.1.8.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.
In GRAM NEGATIVE BACTERIA, multiprotein complexes that function to translocate pathogen protein effector molecules across the bacterial cell envelope, often directly into the host. These effectors are involved in producing surface structures for adhesion, bacterial motility, manipulation of host functions, modulation of host defense responses, and other functions involved in facilitating survival of the pathogen. Several of the systems have homologous components functioning similarly in GRAM POSITIVE BACTERIA.
Rendering pathogens harmless through the use of heat, antiseptics, antibacterial agents, etc.
Use for material on dental facilities in general or for which there is no specific heading.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
The nonexpendable items used by the dentist or dental staff in the performance of professional duties. (From Boucher's Clinical Dental Terminology, 4th ed, p106)
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A type of endoplasmic reticulum (ER) where polyribosomes are present on the cytoplasmic surfaces of the ER membranes. This form of ER is prominent in cells specialized for protein secretion and its principal function is to segregate proteins destined for export or intracellular utilization.
A species of gram-negative bacteria that grows preferentially in the vacuoles of the host cell. It is the etiological agent of Q FEVER.
The interactions between a host and a pathogen, usually resulting in disease.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Therapy by various hot or warm baths in natural mineral waters, spas, or "cures". It includes not only bathing in, but also drinking the waters, but it does not include whirlpool baths (HYDROTHERAPY).
An enzyme that catalyzes the hydrolysis of a single fatty acid ester bond in lysoglycerophosphatidates with the formation of glyceryl phosphatidates and a fatty acid. EC 3.1.1.5.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Substances that reduce the growth or reproduction of BACTERIA.
The fluid inside CELLS.
Water that is intended to be ingested.
Oxidase Variable Legionella pneumophila may be oxidase-positive. OX− normally means the bacterium does not contain cytochrome c ... The end-product of this metabolism is either water or hydrogen peroxide (broken down by catalase). Strains may be either ... Enterobacteriaceae are typically OX−. Wet each disk with about four inoculating loops of deionized water. Use a loop to ...
She has investigated the water-borne pathogen Legionella pneumophila. Swanson is interested in the metabolic cues that underpin ... pneumophila to persist in water, looking to create a microbiology based system to assess the safety of public water systems. ... Ari B. Molofsky; Michele S. Swanson (1 July 2004). "Differentiate to thrive: lessons from the Legionella pneumophila life cycle ... Swanson has studied how Legionella pneumophila remains virulent in different environments. She identified that the pathogen ...
Poor maintenance of air conditioning systems has led to outbreaks of Legionella pneumophila. Hospital-acquired airborne ... Proximity to large bodies of water such as rivers and lakes can be a cause of some outbreaks of airborne disease. ... Also, while respiratory droplets consist mostly of water, airborne particles are relatively dry, which damages many pathogens ...
Legionella bacteria survive in water as intracellular parasites of water-dwelling protozoa, such as amoebae. Amoebae are often ... Similarly, improved diagnosis of human illness related to Legionella species and serogroups other than Legionella pneumophila ... Legionella spp. enter the lungs either by aspiration of contaminated water or inhalation of aerosolized contaminated water or ... Makin, T (January 2008). "Legionella bacteria and solar pre-heating of water for domestic purposes" (PDF). UK Water Regulations ...
"Treatment of a Legionella pneumophila‐Colonized Water Distribution System Using Copper‐Silver Ionization and Continuous ... Copper in renewable energy Copper nanoparticle Erosion corrosion of copper water tubes Cold water pitting of copper tube List ... Copper does not react with water, but it does slowly react with atmospheric oxygen to form a layer of brown-black copper oxide ... In aqueous solution, copper(II) exists as [Cu(H 2O) 6]2+ . This complex exhibits the fastest water exchange rate (speed of ...
The W. magna C2c Maky strain has demonstrated a unique ability to be able to eliminate Legionella pneumophila strains. As the ... pneumophila can be quite an issue for humans as it is often found in cooling tower water. Due to the high temperatures ... It has shown a preference to some particular bacteria such as Legionella pneumophila when it is available. It has been ... L. pneumophila often uses other amoebae as vectors to spread, but W. magna can fully digest the pathogen inside food vacuoles. ...
In the water that had previously stagnated inside the hose, a very aggressive type of legionella pneumophila bacterium had ... Legionella disaster) began on 25 February 1999 in Bovenkarspel, the Netherlands, and was one of the largest outbreaks of ...
According to the official information, the infection was caused by Legionella pneumophila virus, which spread through the water ...
14 June 2017). "Grey water reuse - Assessment of the health risk induced by Legionella pneumophila". Sustainable Earth ... Grey water systems should comply with BS8525 and the Water Supply (Water Fittings) Regulations in order to avoid risks to ... Potable water Rainwater harvesting Water conservation Water purification Behzadian, k; Kapelan, Z (2015). "Advantages of ... The application of grey water reuse in urban water systems provides substantial benefits for both the water supply subsystem, ...
Poorly maintained water cooling towers can promote the growth and spread of microorganisms such as Legionella pneumophila, the ... The chilled water is cooled by chillers in the plant, which use a refrigeration cycle to cool water, often transferring its ... The monoblock type collects the water in a bucket or tray and stops when full. The air-to-air type re-evaporates the water and ... the evaporative cooling effect may be used by placing water at the air intake, such that the draft draws air over water and ...
... and Legionella pneumophila can also cause CAP. Enteric gram-negative bacteria, such as Escherichia coli and Klebsiella ... Typically, a virus enters the lungs through the inhalation of water droplets and invades the cells lining the airways and the ... Chlamydophila pneumoniae or Legionella pneumophila than older children. In contrast, older children and teenagers are more ... Bacteria and fungi typically enter the lungs by inhalation of water droplets, although they can reach the lung through the ...
... it was found that the water in the shower area of the thermal center had high levels of legionella pneumophila. During another ... All the water in the municipality is flowing water. The municipality was the capital of the Yverdon District until it was ... People began to settle, at first in temporary huts at the water-front, for fishers and merchants, then in permanent dwellings. ... It is even possible that the Helvetii appreciated the beneficial effects of these waters. About a century after the first ...
... which are susceptible to becoming breeding grounds for Legionella pneumophila. This system was officially launched on August 17 ... Enwave draws cold water (4 °C/39 °F) from a depth of 83 metres (272 ft) below the surface of Lake Ontario. Cold lake water is ... The water at that depth is a constant 4°C, its temperature protected by a layer of water above it, called a thermocline. The ... Enwave City of Toronto: Environmental initiatives - deep lake water cooling Canadian Urban Institute -- deep lake water cooling ...
... also curtails dependency on evaporative cooling towers which can often times harbor the fatal bacterium Legionella pneumophila ... This cooled water then acts as the heat sink for the heat pump. Deep lake water cooling uses cold water pumped from the bottom ... Deep water source cooling requires a large and deep water quantity in the surroundings. To obtain water in the 3 to 6 °C (37 to ... Water is most dense at 3.98 °C (39.16 °F) at standard atmospheric pressure. Thus as water cools below 3.98 °C it decreases in ...
The bacteria Legionella pneumophila, a facultative intracellular parasite which causes Legionnaires' disease, and Vibrio ... These reservoirs may exist on land (plants and soil), in water, or in the air. Pathogens found in these reservoirs are ... water, blood and fomites can act as passive transmission points between reservoirs and susceptible hosts. Fomites are inanimate ... such as a volume of contaminated air or water. Because of the enormous variety of infectious microorganisms capable of causing ...
Legionella pneumophila has been detected at the point of use downstream from horizontally-mounted electric water heaters with ... Appliances that provide a continual supply of hot water are called water heaters, hot water heaters, hot water tanks, boilers, ... "tempered water". A tempering valve mixes enough cold water with the hot water from the heater to keep the outgoing water ... hot water and water heated to steam have many uses. Domestically, water is traditionally heated in vessels known as water ...
... pneumophila and provided recommendations to improve Legionella prevention. One focus of inquiry is when Snyder became aware of ... This emergency assistance is to provide water, water filters, water filter cartridges, water test kits, and other necessary ... However, the water from the Detroit Water and Sewerage Department, where Flint had obtained its water since 1967, had been ... "Articles on Flint water crisis found using search terms flint water crisis lead ". Detroit Free Press. "Articles on Flint water ...
Reiff, Fred (22 January 2010). "Legionella Control in Institutional Water Systems". Water Quality and Health Council. Retrieved ... Legionella could be used as a weapon, and indeed genetic modification of L. pneumophila has been shown where the mortality rate ... Legionella dies within 32 minutes 55 °C (131 °F) - Legionella dies within 5 to 6 hours Water can be monitored in real-time with ... taking into account both water flow and overall water usage, to control Legionella. The disinfection function within all of a ...
It was isolated from a heated water sample in Minnesota. L. cherrii is similar to another Legionella species, L. pneumophila, ... The genus Legionella is named after the 1976 pneumonia (Legionella pneumophila) outbreak at the American Legion convention at ... Legionella species are mostly found in freshwater environments. However, various strains of Legionella can congregate in water ... Although L. pneumophila is the leading cause of Legionnaire's disease, other Legionella species (such as L. cherrii) likely are ...
"Legionella jordanis: a new species of Legionella isolated from water and sewage". Journal of Clinical Microbiology. 15 (2): 290 ... "Identification of Legionella pneumophila-specific genes by genomic subtractive hybridization with Legionella micdadei and ... "Legionella jordanis: a new species of Legionella isolated from water and sewage". Journal of Clinical Microbiology. 15 (2): 290 ... Legionella jordanis strain BL-540 was first isolated from water samples taken at the Jordan River in Bloomington, Indiana by ...
"Specificity of Legionella pneumophila and Coxiella burnetii vacuoles and versatility of Legionella pneumophila revealed by ... In water systems, the pathogen lives in low metabolic state in the biofilms. A known strain of Legionella called in vitro L. ... Legionella is considered to be pathogenic in humans and is one of the leading outbreaks found in constructed water systems. ... Declerck, Priscilla (2010-03-01). "Biofilms: the environmental playground of Legionella pneumophila". Environmental ...
2006). "Legionella anisa, a Possible Indicator of Water Contamination by Legionella pneumophila". J. Clin. Microbiol. 44 (1): ... After Legionella pneumophila, this species has been isolated most frequently from water samples. This species is also one of ... 1985). "Legionella anisa: a new species of Legionella isolated from potable waters and cooling tower". Appl Environ Microbiol. ... Thus, L. anisa, along with several other species of Legionella, is sometimes referred to as "blue-white" Legionella. Legionella ...
"Legionella pneumophila cases" (PDF). Docs2.health.vic.gov.au. Retrieved 2015-08-30. "Health (Legionella) Regulations 2001 S.R. ... Storage of Preheated Domestic Hot Water and Possible Growth of Legionella Bacteria" (PDF). The Water Regulations Advisory ... ISBN 978-0-683-30740-5. Lin YE, Stout JE, Yu VL (February 2011). "Controlling Legionella in hospital drinking water: an ... Malta requires twice yearly testing for Legionella bacteria at cooling towers and water fountains. Malta prohibits the ...
Legionella, and in particular the most virulent strain, Legionella pneumophila serogroup 1, can cause infections when inhaled ... oily bilge water, ballast water, and solid waste. They also emit air pollutants to the air and water. These wastes, if not ... However, they have added amenities to cater to water tourists, with recent vessels being described as "balcony-laden floating ... Causes of E. coli infection include the consumption of contaminated food and the ingestion of water in swimming pools ...
... and Legionella pneumophila. For these nonstandard indications, antimicrobial susceptibility testing should be done (if possible ... Rifampicin should be taken on an empty stomach with a glass of water. It is generally taken either at least an hour before ... redness and watering of eyes Abdominal - nausea, vomiting, abdominal cramps, diarrhea Flu-like symptoms-chills, fever, headache ...
Species of Legionella known to cause Pontiac fever include Legionella pneumophila, Legionella longbeachae, Legionella feeleii, ... It is acquired through aersolization of water droplets and/or potting soil containing Legionella bacteria. Pontiac fever is ... Legionella micdadei, and Legionella anisa. Sources of the causative agents are aquatic systems and potting soil. The first ... "Legionella (Legionnaires' Disease and Pontiac Fever". CDC. Centers for Disease Control and Prevention. Retrieved 4 June 2014. ...
Legionella pneumophila, and Candida albicans and is considered to be a very fast-acting, broad-spectrum microbicidal solution. ... Hall, Harriet (August 2012). "ASEA: Another Expensive Way to Buy Water". Science Based Medicine. Hall, Harriet (November 13, ... and Legionella pneumophila". American Journal of Infection Control. 28 (3): 251-257. doi:10.1067/mic.2000.105287. PMID 10840346 ... and Legionella pneumophila". American Journal of Infection Control. 28 (3): 251-257. doi:10.1067/mic.2000.105287. PMID 10840346 ...
The bacterium was later named Legionella pneumophila. Legionella pneumophila is the most common cause, but sometimes other ... "Spread by airborne droplets? Legionnaire's disease linked to water". The Globe and Mail. September 4, 1978. p. 10. Retrieved ... 1999 Bovenkarspel legionellosis outbreak Legionella pneumophila Legionnaires' disease List of Legionellosis outbreaks Klaus ... occasion in which a cluster of a particular type of pneumonia cases were determined to be caused by the Legionella pneumophila ...
Buffered charcoal yeast extract agar is selective for certain Gram-negative bacteria, especially Legionella pneumophila. ... water. Supplementary minimal media are minimal media that also contains a single selected agent, usually an amino acid or a ...
... groundwater or drinking water supplies. Drinking any contaminated water and eating any foods washed in the water, as well as ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ... Water purification: All water used for drinking, washing, or cooking should be sterilized by either boiling, chlorination, ... The wealthy elite of the country were able to afford safe water while others had to use water from cholera-infected rivers.[120 ...
Emergency relief services often distribute inexpensive packets of sugars and mineral salts that can be mixed with clean water ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ... Treatment often starts with an oral rehydrating solution-water mixed with salt and carbohydrates-to prevent dehydration. ( ...
"Isolation of Legionella pneumophila from clinical specimens via amoebae, and the interaction of those and other isolates with ... Megavirus was isolated from a water sample collected in April 2010 off the coast of Chile, near the marine station in Las ...
Haemophilus influenzae/Legionella pneumophila *Buffered charcoal yeast extract agar. *Pseudomonas aeruginosa *Cetrimide agar ... dissolved in 1 liter of distilled water.. *pH adjusted to neutral at 25 °C.[1] ...
Legionella, and in particular the most virulent strain, Legionella pneumophila serogroup 1, can cause infections when inhaled ... oily bilge water, ballast water, and solid waste. They also emit air pollutants to the air and water. These wastes, if not ... Other pathogens which can colonise pools and spas including those on cruise ships include Legionella, the bacteriium which ... and the waters of Alaska), their cumulative impact on a local scale could be significant, as can impacts of individual large- ...
Water[edit]. Although safe bottled water is now widely available in most remote destinations, travelers can treat their own ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ... Safe beverages include bottled water, bottled carbonated beverages, and water boiled or appropriately treated by the traveler ( ... reports of locals filling empty bottles with untreated tap water and reselling them as purified water have surfaced.[10] When ...
"Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection". Journal of Visualized Experiments ( ... whereas aquatic toxicologists may analyze the toxicity level of water bodies. ...
... while others are found in water or soil, or are parasites on a variety of different animals and plants. ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ...
Daily water-traffic with the nearby city of Hong Kong rapidly spread the plague there, killing over 2,400 within two months ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ...
Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ... Water Research. 31 (3): 661-664. doi:10.1016/S0043-1354(96)00094-2.. ...
Poorly maintained water cooling towers can promote the growth and spread of microorganisms such as Legionella pneumophila, the ... so an intermediate fluid such as chilled water is used instead. The plant circulates cold water to terminal chilled water ... The monoblock type collects the water in a bucket or tray and stops when full. The air-to-air type re-evaporates the water and ... In addition, if water is condensed in the room, the amount of heat previously needed to evaporate that water also is re- ...
ingestion of infested water plants or water (intermediate host:amphibic snails) Metagonimiasis - intestinal fluke Metagonimus ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ... skin exposure to water contaminated with infected Oncomelania sp. snails Asian intestinal schistosomiasis Schistosoma mekongi ... ingestion of water containing deer or beaver feces Isosporiasis Isospora belli epithelial cells of small intestines stool ...
The first permanent disinfection of drinking water in the U.S. was made to the Jersey City, New Jersey, water supply. Credit ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ... "Description of the Process and Plant of the Jersey City Water Supply Company for the Sterilization of the Water of the Boonton ... In industrialized nations, water sanitation and food handling improvements have reduced the number of cases.[49] Developing ...
"They examined nonculturability and resuscitation in Legionella Pneumophila and while entry into this state was easily induced ... Intracellular crowding refers to the fact that protein function and interaction with water is constrained when the interior of ...
... chewing on toys and even through sharing a source of fresh water. It infects its host cells by sticking to them with long thin ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ...
fecal-oral transmission - usually from contaminated food or water sources. *vector borne transmission - carried by insects or ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ... to contaminate enemy water supplies. Han Dynasty General Huo Qubing is recorded to have died of such a contamination while ...
They are usually spread by eating or drinking food or water contaminated with the feces of an infected person.[1] They may ... Legionella pneumophila/Legionella longbeachae *Legionnaires' disease. *Coxiella burnetii *Q fever. Thiotrichales. *Francisella ... "Water-related Diseases." Communicable Diseases 2001. World Health Organization. 31 Oct 2008 ,. "Archived copy". Archived from ... Providing basic sanitation and safe drinking water and food are the keys for controlling the disease. In developed countries, ...
"Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection". Journal of Visualized Experiments ( ... Heat treatment keeps the combs and beekeeping equipment at 45-80 °C for 1-4 hours, or in hot water for 3-5 hours. However, ...
2012 Water quality - Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic ... 2011 Water quality - Measurement of polonium 210 activity concentration in water by alpha spectrometry ISO 13162:2011 Water ... 2012 Water quality - Strontium 90 and strontium 89 - Test methods using liquid scintillation counting or proportional counting ... 2015 Water quality - Plutonium, americium, curium and neptunium - Test method using alpha spectrometry ISO/IEC 13170:2009 ...
... -positive pathogens, such as Mycobacterium tuberculosis, Legionella pneumophila, and Campylobacter jejuni, make ... The free oxygen atom coordinates, freeing the newly formed water molecule and Fe(IV)=O. Fe(IV)=O reacts with a second hydrogen ... which catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the ... one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second. Catalase is a ...
... Masato Tachibana,1,2 Masaya ... "Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, ... S. L. G. Cirillo, L. E. Bermudez, S. H. El-Etr, G. E. Duhamel, and J. D. Cirillo, "Legionella pneumophila entry gene rtxA is ... R. M. Ratcliff, M. A. Slavin, N. Sangster, R. M. Doyle, J. F. Seymour, and J. A. Lanser, "Legionella pneumophila mip gene ...
Legionella pneumophila, the causative agent of Legionnaires disease, is known to colonise and frequently grow in cooling tower ... Article Enumeration of Legionella pneumophila in cooling tower water systems. ... Enumeration of Legionella pneumophila in cooling tower water .... Enumeration of Legionella pneumophila in cooling tower water ... No comments were found for Enumeration of Legionella pneumophila in cooling tower water systems. Be the first to comment! ...
Legionella pneumophila,/i, and ,i,mip,/i, gene separately; PCR for detecting ,i,E. coli,/i,; and another PCR for detecting ... Legionella pneumophila,/i,, and ,i,E. coli,/i, in Gilan, Iran. Samples were collected directly into sterile containers and ... Degrees of contamination with ,i,L. pneumophila,/i, were 3.3%, 9.3%, and 10.9% and with ,i,E. coli,/i, were zero, 6.2%, and ... Contamination degree with ,i,P. aeruginosa,/i, was considerable and with ,i,L. pneumophila,/i, was moderate. Metallo-,i,β,/i,- ...
Article Removing Legionella pneumophila and biofilms from water supply systems using plant essential oils. Antibacterial and ... Removing Legionella pneumophila and biofilms from water supply systems using plant essential oils. 0 ... No comments were found for Removing Legionella pneumophila and biofilms from water supply systems using plant essential oils. ... Legionella pneumophila. . Results showed that all EOs had antibacterial activity. Lemon oil demonstrated the best activity, ...
Water heater temperature set point and water use patterns influence Legionella pneumophila and associated microorganisms at the ... Legionella Isolate Characterization. Of the 515 total water samples collected and from which L. pneumophila isolation was ... and L. pneumophila genes were found to be higher in the tap water of large buildings in Flint than in other water systems in US ... Persistent Legionella pneumophila colonization of a hospital water supply: efficacy of control methods and a molecular ...
Comparison of Whole-Genome Sequences of Legionella pneumophila in Tap Water and in Clinical Strains, Flint, Michigan, USA, 2016 ... Comparison of Whole-Genome Sequences of Legionella pneumophila in Tap Water and in Clinical Strains, Flint, Michigan, USA, 2016 ... Second letter indicates sample collection location; H, hot water tap; C, cold water tap; D, water heater drain valve; S, shower ... 3 hospital water (HH17, HH25, HH56), 1 residence water (RH08), 3 clinical (C2, C3, C7). ...
Aerosols containing Legionella pneumophila generated by shower heads and hot-water faucets.. G E Bollin, J F Plouffe, M F Para ... Shower heads and hot-water faucets containing Legionella pneumophila were evaluated for aerosolization of the organism with a ... Aerosols containing Legionella pneumophila generated by shower heads and hot-water faucets. ... Aerosols containing Legionella pneumophila generated by shower heads and hot-water faucets. ...
Water systems of large buildings, such as hospitals, are often contaminated with legionellae and therefore represent a ... Introduction: Legionnaires disease is normally acquired by inhalation of legionellae from a contaminated environmental source ... Prevalence of Legionella pneumophila in water distribution systems in hospitals and public buildings of the Lublin region of ... Nosocomial Legionella pneumophila serogroup 5 outbreak associated with persistent colonization of a hospital water system. Acta ...
The study aims to carry out a three-year retrospective review of routine Legionella follow-up analyses in various samples... ... Prevalence of Legionella pneumophila in water distribution systems in hospitals and public buildings of the Lublin region of ... Sepin-Ozen N, Tuglu-Ataman S, Emek M. Exploring the Legionella pneumophila positivity rate in hotel water samples from Antalya ... Investigation of the presence of Legionella pneumophila in water samples from Erzurum and surrounding provinces in Turkey ...
GS detected L. pneumophila in 94% and 100% of env … ... were examined for the presence of Legionella pneumophila with a ... Sixty-three environmental water samples from various sources ... Detection of Legionella pneumophila in environmental water ... Sixty-three environmental water samples from various sources were examined for the presence of Legionella pneumophila with a ... Cultures of L. pneumophila serogroups 1 to 12, 14 and non-L. pneumophila bacteria which may be found in water, and bacteria ...
Legionella drozanskii sp. nov., Legionella rowbothamii sp. nov. and Legionella fallonii sp. nov.: three unusual new Legionella ... drinking water from the tap of room D0.04 was analyzed by using Legionella genus-specific and Legionella pneumophila-specific ... pneumophila genome units/liter of bulk water) in the cold-water samples but relatively high numbers in the hot-water samples ( ... For the quantification of the number of Legionella pneumophila genes in drinking water samples, real-time PCR using Sybr green ...
... to see what factors might influence the prevalence of Legionella pneumophila. The effect on the organism of three anti- ... Fourteen recirculating cooling water systems were surveyed during the summer, 1981, ... Legionella pneumophila in cooling water systems. Report of a survey of cooling towers in London and a pilot trial of selected ... to see what factors might influence the prevalence of Legionella pneumophila. The effect on the organism of three anti- ...
... thermophilic amoebae and Legionella pneumophila - a quantitative risk assessment for distributed water. Water Sci Technol 1 ... thermophilic amoebae and Legionella pneumophila - a quantitative risk assessment for distributed water. Water Sci Technol 1 ... Biofilms, thermophilic amoebae and Legionella pneumophila - a quantitative risk assessment for distributed water M.V. Storey M. ... was developed for Legionella within a water distribution system. Both biofilms and a thermophilic isolate of acanthamoebae were ...
Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by ... Water quality - Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic ... 1ST EDITION - Water quality - Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration ... 2ND EDITION - Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic ...
Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by ... Détection et quantification de Legionella spp. et/ou Legionella pneumophila par concentration et amplification génique par ... Water quality. Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic ... Water quality. Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic ...
... pneumophila in water samples including water sanitation plants, reverse-osmosis water supplies and samples of tap water from ... of samples from two hot water systems containing Legionella pneumophila compared with drinking water from municipal water works ... pneumophila in crude water sources and in general drinking water supplies and tankers of drinking water. The prevalence of L. ... pneumophila in sources of crude water, in general drinking water supplies and drinking water tankers. Of 258 isolates 77.1% ...
Removing Legionella pneumophila and biofilms from water supply systems using plant essential oils. Journal of Water, Sanitation ... Removing Legionella pneumophila and biofilms from water supply systems using plant essential oils. Journal of Water, Sanitation ... Removing Legionella pneumophila and biofilms from water supply systems using plant essential oils Ozgur Ceylan Ozgur Ceylan ... Inactivation of Legionella pneumophila from whirlpool bath waters by grapefruit (Citrus paradisi) seed extract ...
Legionella pneumophila DNA standard. The DNA used for reaction validation and as reference for Lp quantification in water ... In an attempt to provide a simple screening method for Legionella pneumophila in water systems samples a real time PCR assay ... The objective of this study was to develop a simple, screening real-time PCR assay for Legionella pneumophila in water systems ... In this report, we described a simple, sensitive, reliable realtime PCR method for detecting Legionella pneumophila in water ...
Waters - Examination for legionellae including Legionella pneumophila. This document has been re-assessed by the committee, and ... Waters - Examination for Legionella spp. including Legionella pneumophila. Get the latest version of this product ... Specifies a method for detecting and estimating the number of Legionella pneumophila and some other legionellae in water. In ... Air-handling and water systems of buildings - Microbial control - Part 4: Performance-based maintenance of air-handling systems ...
Water heater temperature set point and water use patterns influence Legionella pneumophila and associated microorganisms at the ... Survey of green building water systems reveals elevated water age and water quality concerns. Environ Sci Water Res Technol. ... In particular, Legionella pneumophila is an OP that has become the leading cause of drinking-water-related disease in developed ... Legionella and Mycobacterium in tap water supplied by a municipal drinking water utility in the USA. J Water Health. 2010;8:71- ...
EUs new drinking water law is in line with latest science, health expert says. An EU agreement to monitor tap water for the ... New tap water rules already flowing ahead of EU rubber stamp. While waiting for the go-ahead to the overhauled Drinking Water ... Providing tap water with the same level of information and transparency as bottled water will restore citizens trust and help ... ECDC: Legionella outbreak may be reduced with appropriate controls. A new community outbreak of Legionella in Belgiums ...
A PCR-Based method for monitoring Legionella pneumophila in water samples detects viable but noncultivable legionellae that can ... Legionella pneumophila and Legionella spp. cultivation. For quantification of Leg. pneumophila and Legionella spp. via ... a) Legionella pneumophila and Legionella spp. concentrations determined via cultivation and L. pneumophila via CARD-FISH. The ... a) Legionella pneumophila and Legionella spp. concentrations determined via cultivation and L. pneumophila via CARD-FISH. The ...
This study determined whether Legionella pneumophila can colonize and grow in biofilms with and without an association with ... Growth of legionellae in the absence of protozoa has been documented only on complex laboratory media. The hypothesis upon ... The levels of L. pneumophila cells present in the biofilm were monitored for 15 d, with and without the presence of H. ... vermiformis, and it was found that, although unable to replicate in the absence of H. vermiformis, L. pneumophila was able to ...
For determining whether this was the result of relapse or reinfection, Legionella pneumophila strains, cultured from sputum ... Legionella pneumophila strains, cultured from sputum specimens and his home water supply, were characterized by pulsed-field ... Legionella pneumophila / genetics, isolation & purification*. Legionnaires Disease / diagnosis*, drug therapy, microbiology. ... culturing of the domestic water supplies for L. pneumophila should be considered; if such cultures are positive, adequate ...
Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower. Appl. Environ. Microbiol. 49 : ... The importance of type II secretion for Legionella pneumophila survival in tap water and amoebae at low temperature. Appl. ... The genus Legionella was established in 1977, following the isolation of Legionella pneumophila from patients with a form of ... Legionella moravica sp. nov. and Legionella brunensis sp. nov. isolated from cooling-tower water. Ann. Inst. Pasteur Microbiol. ...
Legionella pneumophila. 6. Six cases of legionellosis were reported to the local health department by a long term care facility ... Water testing from multiple water sources at the facility was negative for Legionella, though delays in completing this testing ... water temperature reaching prime Legionella growth range, and high levels of humidity resulting in longer suspension of water ... Legionella pneumophila serogroup 1. 22. A contaminated air handler system at a religious center resulted in 22 cases of ...
Water management programs are an important way to help reduce the risk of transmission infection of these organisms among ... Water can harbor germs that threaten the safety of patients. ... Legionella pneumophila, Mycobacaterium avium, and Pseudomonas ... Preventing Legionnaires Disease: A Training on Legionella Water Management Programs. *Safe Water, Safe Dentistry, Safe Kids [ ... Healthcare Water Management. Meeting water quality standards, limiting infection risks from water sources, and designing and ...
... the gold standard typing method for Legionella pneumophila, in the analysis of endemic strains. Recently, a gene-by-gene typing ... pneumophila. Methods: We applied this cgMLST scheme to isolates obtained during the largest outbreak of LD reported so far in ... Specific real-time PCR for simultaneous detection and identification of Legionella pneumophila serogroup 1 in water and ... Legionella pneumophila sequence type 1/Paris pulsotype subtyping by spoligotyping. J Clin Microbiol. 2012;50(3):696-701. https ...
Find a laboratory that uses the Legiolert Test to detect and quantify Legionella pneumophila, the primary cause of Legionnaires ... The ISO designation includes hospital waters, drinking water and swimming/spa pool waters. ... Water International. Australia. Brazil. Canada. China. France. Germany. Italy. Japan. Latin America. Spain. Taiwan. United ... the Surfrider Foundation Rincón Chapters Blue Water Task Force is even more committed to providing critical water testing ...
Here, we employed a lab-scale recirculating hot water plumbing rig to compare the pre- and post- ... Heat shock is a potential control strategy for Legionella pneumophila in hot water plumbing systems. However, it is not ... Water heater temperature set point and water use patterns influence Legionella pneumophila and associated microorganisms at the ... Hot water systems are especially vulnerable to the growth of opportunistic pathogens (OPs), such as Legionella pneumophila and ...
  • Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia," Journal of Medical Microbiology , vol. 55, no. 8, pp. 993-997, 2006. (hindawi.com)
  • L. pneumophila serogroup 1 was found in 44% of the isolated strains, which is primarily responsible for the majority of Legionnaires disease. (environmental-expert.com)
  • Isolates belonging to 1 clade (3 clinical isolates, 3 water isolates from a Flint hospital, 1 water isolate from a Flint residence, and the reference Paris strain) had a high degree of similarity (2-1,062 single-nucleotide polymorphisms), all L. pneumophila sequence type 1, serogroup 1. (cdc.gov)
  • Serogroup 6 isolates belonging to sequence type 2518 were widespread in Flint hospital water samples but bore no resemblance to available clinical isolates. (cdc.gov)
  • Among the 15 serogroups of L. pneumophila , serogroup 1 accounts for 84% of confirmed cases, as assessed by an international collaborative survey ( 40 ). (asm.org)
  • The prevalence of L. pneumophila, especially serogroup 1, is a strong indicator of unsuitability of drinking water and requires appropriate action. (who.int)
  • Legionella pneumophila Serogroup 1 is the most common pathogenic species and is responsible for up to 80% of legionellosis cases in the world. (scielo.org.za)
  • Legionella pneumophila Serogroup 1 (NCTC12821) was used as a reference strain and to evaluate real-time PCR performance. (scielo.org.za)
  • The majority of the community- acquired cases are caused by strains belonging to Legionella pneumophila serogroup 1 [3,4]. (jacobspublishers.com)
  • L. pneumophila isolate genomes belonging to serogroup 1 were identified via detection of the wzm gene ( 11 ) in whole genomes using BLAST with a minimum nucleotide identity of 98% and e-value of 1e-5. (cdc.gov)
  • Epidemiological Investigation of Legionella pneumophila Serogroup 2 to 14 Isolates from Water Samples by Amplified Fragment Length Polymorphism and. (cdc.gov)
  • Eighty L. pneumophila serogroup 2 to 14 strains isolated from water distribution systems of hotels, hospitals, athletic venues, and ferries in Greece were tested by monoclonal antibodies (MAbs) for serogroup discrimination and molecularly by amplified fragment length polymorphism (AFLP) for genetic diversity. (cdc.gov)
  • Thirty-nine different AFLP types were found among the 80 L. pneumophila serogroup 2 to 14 strains, and 24 different SBT types were found among the 56 strains tested. (cdc.gov)
  • pneumophila serogroup 1 in the water from 2 of the 4 trucks sampled. (thefreedictionary.com)
  • Photocatalytic oxidation (PCO) was proven to be efficacious in the inactivation of Legionella pneumophila serogroup 1 Strains 977, 1009, 1014 and ATCC 33153. (semanticscholar.org)
  • To evaluate the genetic diversity of Legionella pneumophila in Southeast Michigan, we determined the sequence type (ST) and serogroup (SG) of the 18 residential isolates from Flint and Detroit, MI, and the 33 clinical isolates submitted by hospitals in three area counties in 2013 to 2016. (asm.org)
  • Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. (atcc.org)
  • Z30431 L.pneumophila Serogroup 1 Philadelphia-1 gene for 23S ribosomal RNA and 5S ribosomal RNA. (atcc.org)
  • It is conjectured that up to 40% of cases involving these rare pathogens, attributed to species and groups other than L. pneumophila serogroup 1, are missed clinically if commercially available urine antigen detection kits, which target only L. pneumophila serogroup 1, are used exclusively for diagnosis(4). (msdsonline.com)
  • The most common causative agent of LD is L. pneumophila serogroup 1 (Lp1)(9,10). (msdsonline.com)
  • Legionella pneumophila serogroup 1 has been present for up to 10 years in the potable water supply of five of six health care institutions in the city of Halifax, Nova Scotia, Canada ( 1 ). (asm.org)
  • At hospital B, all of the isolates from both patients and water samples have been L. pneumophila serogroup 1, which lacks plasmids ( 1 ). (asm.org)
  • L. pneumophila serogroup 1 strains which had been isolated and typed as previously described ( 5 ) were recovered from storage. (asm.org)
  • Legionnaires' disease is normally acquired by inhalation or aspiration of Legionella pneumophila serogroup 1 from a contaminated environmental source. (asm.org)
  • For sensitivity testing and construction of a standard curve, samples from a 1-liter microcosm containing L. pneumophila serogroup 1 were prepared and the viable count was determined by plating out 100-μl portions of appropriate dilutions on buffered charcoal yeast extract agar plates ( 5 ). (asm.org)
  • A large outbreak of Legionnaires' disease due to an inadequate circulating and filtration system for bath water- epidemiologic manifestations," Internal Medicine , vol. 42, no. 9, pp. 806-811, 2003. (hindawi.com)
  • Legionella pneumophila, the causative agent of Legionnaires disease, is known to colonise and frequently grow in cooling tower waters. (environmental-expert.com)
  • During the water crisis in Flint, Michigan, USA (2014-2015), 2 outbreaks of Legionnaires' disease occurred in Genesee County, Michigan. (cdc.gov)
  • Legionnaires' disease is a severe form of pneumonia caused by inhalation of virulent species of aerosolized Legionella bacteria. (cdc.gov)
  • This disruption in water quality likely also stimulated the growth of L. pneumophila , the species most frequently identified as the causative agent of Legionnaires' disease ( 5 , 6 ), in Flint's distribution and plumbing systems ( 7 ). (cdc.gov)
  • Our prior work associated the Legionnaires' disease outbreaks with factors known to be conducive to Legionella growth: elevated iron (a consequence of corroded iron water mains), reduced free chlorine disinfectant residuals, and elevated water temperatures ( 7 , 8 ). (cdc.gov)
  • Later, Zahran and colleagues reported that the odds of Flint residents being referred for Legionnaires' disease treatment while the Flint River was the source of tap water increased 6.3-fold and confirmed our report of associations with low chlorine residuals ( 9 ), but the odds analysis, which was based on the use of referral date rather than symptom onset date, excluded many healthcare-associated cases ( 10 ). (cdc.gov)
  • Legionnaires' disease is normally acquired by inhalation of legionellae from a contaminated environmental source. (aaem.pl)
  • Hospital characteristics associated with colonization of water systems by Legionella and risk of nosocomial legionnaires' disease: a cohort study of 15 hospitals. (aaem.pl)
  • Legionnaires' disease caused by Legionella pneumophila serogroups 5 and 10, China. (aaem.pl)
  • Legionella pneumophila is a Gram-negative, facultative, intracellular pathogen that accounts for the majority of cases of Legionnaires' disease in Europe ( 16 ). (asm.org)
  • 2004). Between 1995 and 2005, a total of 4 056 culture-confirmed cases of Legionnaires' disease from European countries were notified to the European Working Group for Legionella Infections (EWGLI) (Diereden, 2008). (scielo.org.za)
  • A warmer climate and Europe's ageing population create a favourable breeding ground for legionella bacteria, which cause a type of pneumonia known as Legionnaires' disease, Professor Martin Exner said in an interview. (euractiv.com)
  • A new community outbreak of Legionella in Belgium's Flanders region renewed public attention to the so-called Legionnaires' disease, which is increasing in the EU, according to the EU agency for infectious diseases control. (euractiv.com)
  • The overwhelming majority of cases of Legionnaires' disease are caused by Legionella pneumophila ( Joseph and Ricketts 2010 ). (wiley.com)
  • The genus Legionella was established in 1977, following the isolation of Legionella pneumophila from patients with a form of pneumonia now known as Legionnaires' disease ( 33 ). (asm.org)
  • L. pneumophila is especially pathogenic for the elderly and the immunocompromised, large and growing segments of the population ( 39 , 84 ), and recent studies have been highlighting the growing significance of travel-associated Legionnaires' disease ( 107 ). (asm.org)
  • Whole genome sequencing (WGS) is increasingly used in Legionnaires' disease (LD) outbreak investigations, owing to its higher resolution than sequence-based typing, the gold standard typing method for Legionella pneumophila, in the analysis of endemic strains. (eurosurveillance.org)
  • Find a laboratory that uses the Legiolert Test to detect and quantify Legionella pneumophila , the primary cause of Legionnaires' disease. (idexx.com)
  • Potable water systems have often been implicated as the source in the outbreak of Legionnaires' disease [1,2]. (jacobspublishers.com)
  • Legionella pneumophila, the agent of Legionnaires' disease pneumonia, is transmitted to humans following the inhalation of contaminated water droplets. (nih.gov)
  • Coinciding with major changes to its municipal water system, Flint, MI, endured Legionnaires' disease outbreaks in 2014 and 2015. (asm.org)
  • Because the widely used urinary antigen diagnostic test does not readily detect non-SG1 L. pneumophila , Legionnaires' disease caused by SG6 L. pneumophila is likely underreported worldwide. (asm.org)
  • One year after two outbreaks of Legionnaires' disease in Genesee County, MI, that coincided with damage to the Flint municipal water system, our multidisciplinary team launched an environmental surveillance and laboratory research campaign aimed at informing risk management strategies to provide safe public water supplies. (asm.org)
  • Doctors who have dealt with Legionnaires' disease for decades say that those with a weak immune system or who smoke should take precautions such as boiling drinking water. (theepochtimes.com)
  • Fields BS, Benson RF, Besser RE (2002) Legionella and Legionnaires' disease: 25 years of investigation. (springer.com)
  • Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. (atcc.org)
  • Legionnaires' disease is contracted by breathing in small droplets of contaminated water. (thetimes.co.uk)
  • Legionella pneumophila is a gram-negative, facultative intracellular bacterium and the causative agent of Legionnaires' pneumonia. (asm.org)
  • Legionnaires' pneumonia results when L. pneumophila is inhaled as an aerosol by a susceptible individual. (asm.org)
  • citation needed] L. pneumophila can cause sporadic outbreaks of Legionnaires' disease. (wikipedia.org)
  • PATHOGENICITY/TOXICITY: L. pneumophila infection can cause Legionnaires' disease, a severe form of pneumonia(4,5). (msdsonline.com)
  • Acute bilateral pneumonia (Legionnaires' Disease caused by Legionella pneumophila), seen on a ... [+] frontal chest x-ray. (forbes.com)
  • Testing has revealed Legionella , the bacteria that causes Legionnaires Disease, in hot tubs, water heaters, and shower and sink heads at the hotel. (forbes.com)
  • The bacteria subsequently spread to different rooms via water droplets in the air, going to the Legionnaires' lungs where the bacteria could cause a pneumonia. (forbes.com)
  • Legionnaires' Disease is a more severe Legionella infection that results in pneumonia. (forbes.com)
  • They were looking for legionella bacteria, the organism that causes legionnaires' disease . (telegraph.co.uk)
  • In most cases it wasn't possible to identify the exact species, but in a couple of cases L. pneumophila (the species that causes legionnaires' disease) was positively identified. (telegraph.co.uk)
  • See also Legionella pneumophila , Legionnaires' disease . (thefreedictionary.com)
  • In humans, Legionella is responsible for the so-called Legionnaires' disease, a form of bacterial pneumonia that is often lethal. (bio-medicine.org)
  • Legionnaires' disease-risk estimation may be compromised by uncertainties in Legionella -detection methods. (dovepress.com)
  • A wide variety of opportunistic pathogens such as Pseudomonas can be incorporated into the piped potable water, colonizing the surfaces of pipelines with high numbers of biofilm-forming bacteria [ 10 ]. (hindawi.com)
  • A Major Commercial complex with 5,500 RT HVAC system using BCP 1015 (Classic DTEA II ) for: Legionella Bacteria Control Program (BCP) Biofilm Control Program (BCP) Cleaner/Penetrant Aid/Dispersant Cooling System Before Application of BCP 1015 (Classic DTEA II) Cooling System After Application of BCP 1015 (Classic DTEA II) Legionella Bacteria levels Before BCP (Biofilm Control Program) Legionella Bacteria levels Before BCP (Biofilm Control Program) Conclusion Chemical treatment program before. (environmental-expert.com)
  • Kusnetsov J, Torvinen E, Perola O, Nousiainen T, Katila ML. Colonization of hospital water systems by Legionellae, mycobacteria and other heterotrophic bacteria potentially hazardous to risk group patients. (aaem.pl)
  • Cultures of L. pneumophila serogroups 1 to 12, 14 and non-L. pneumophila bacteria which may be found in water, and bacteria containing non-specific binding proteins, were stained by GS and IFAT. (nih.gov)
  • GS identified all serogroups of L. pneumophila and did not cross react with any non-L. pneumophila bacteria. (nih.gov)
  • An EU agreement to monitor tap water for the presence of potentially lethal Legionella bacteria takes into consideration the emergence of newer, more effective testing methods, a national public health expert told EURACTIV. (euractiv.com)
  • Certain numbers and types of bacteria and other microbes may be present when water leaves the tap. (cdc.gov)
  • Based on its abilities to disperse water droplets and to promote the spreading of heterologous bacteria, the film appeared to manipulate surface tension and, as such, acted like a surfactant. (asm.org)
  • While heat shock may be effective for instantaneous Legionella control and reduction in total bacteria numbers, water heater temperature set point and water use frequency are more promising factors for long-term Legionella and microbial community control, illustrating the importance of maintaining consistent elevated temperatures in the system relative to short-term heat shock. (biomedcentral.com)
  • During analysis of Legionella pneumophila genome sequence, the team from the Biology of Intracellular Bacteria Unit (Institut Pasteur/CNRS), headed by Carmen Buchrieser, identified genes coding for proteins that were predicted to be involved in the infection of human cells. (pasteur.fr)
  • Dr. Faucher is studying the tools use by bacteria to infect humans and animals through consumption of water and food. (mcgill.ca)
  • It seems that the high temperature of hot water system and the high rate of free residual chlorine in tap water system are the main causes of low rate of Legionella contamination but are ineffective on contamination with other bacteria. (who.int)
  • Here, we demonstrate that biofilm-derived L. pneumophila replicates significantly more in murine macrophages than planktonic bacteria. (frontiersin.org)
  • In addition, while planktonic L. pneumophila is promptly delivered to lysosomes for degradation, most biofilm-derived bacteria were enclosed in a vacuole that did not fuse with lysosomes in murine macrophages. (frontiersin.org)
  • It is caused by Legionella bacteria. (medlineplus.gov)
  • The bacteria that cause Legionnaire disease have been found in water delivery systems. (medlineplus.gov)
  • Most cases are caused by the bacteria Legionella pneumophila . (medlineplus.gov)
  • Therefore, we examined the ability of L. pneumophila (clinical isolate 130 b) to persist within biofilms formed by various types of aquatic bacteria, using a bioreactor with flow, steel surfaces, and low-nutrient conditions. (nih.gov)
  • In contrast to these results, L. pneumophila 130 b did not persist within a biofilm formed by Pseudomonas aeruginosa, confirming that some bacteria are permissive for Legionella colonization whereas others are antagonistic. (nih.gov)
  • During a routine check, legionella bacteria were found in a washbasin in the Research Lab of the University of Groningen/UMCG. (rug.nl)
  • Colorized scanning electron micrograph (SEM) of Legionella pneumophila bacteria. (theepochtimes.com)
  • It may be concerning that Lincoln Hospital was one of the two locations that were found positive for the Legionella bacteria. (theepochtimes.com)
  • Lincoln Hospital in the Bronx where Legionella bacteria was found on a rooftop cooling system. (theepochtimes.com)
  • Since the Legionella bacteria grow and thrive in lungs, smokers and those who have lung disease are at risk for this illness. (theepochtimes.com)
  • Legionella bacteria is not transmitted from person to person. (theepochtimes.com)
  • Establishing whether or not water is safe requires robust techniques to isolate and to identify potentially pathogenic bacteria. (bruker.com)
  • The widespread closure of public buildings and workplaces has created ideal conditions for waterborne bacteria, such as Legionella , which attacks the lungs, to thrive. (thetimes.co.uk)
  • Phagosomes carrying virulent L. pneumophila are significantly less acidic than those bearing nonpathogenic bacteria ( 21 ). (asm.org)
  • The tissue was stained using Dieterle's silver stain, which employs a silver-impregnation staining technique, the results of which reveals the presence of Legionella pneumophila bacteria. (news-medical.net)
  • They are present in small water droplets that are contaminated with the bacteria. (news-medical.net)
  • Legionella bacteria are commonly found in sources of water, such as rivers and lakes. (news-medical.net)
  • For prevention of Legionella bacteria from growing and causing disease, water works are guided by regulations and there are guidelines for maintenance and control of water supply systems. (news-medical.net)
  • They have to keeping the water cooled below 20ºC or heated above 60ºC (below 68ºF or above 140ºF) to prevent the bacteria from growing. (news-medical.net)
  • Has Legionella become more common or has the bacteria just gotten better PR people? (forbes.com)
  • The bacteria can enter your lungs when you breathe in either water droplets or soil contaminated with the Legionella . (forbes.com)
  • pneumophila bacteria. (forbes.com)
  • UV disinfects water containing bacteria and viruses and can be effective against protozoans like, Giardia lamblia cysts or Cryptosporidium oocysts. (water-research.net)
  • Class A point-of-entry and point-of-use systems covered by this Standard are designed to inactivate and/or remove microorganisms, including bacteria, viruses, Cryptosporidium oocyst and Giardia cysts, from contaminated water. (water-research.net)
  • To minimize the development of biofilms, hospitals must limit the level of free-floating bacteria in the water supply and perform reliable and continuous disinfection of the water supply. (clinicaladvisor.com)
  • De Blasio said the city has ordered the managers of all buildings with the large cooling towers that can harbor Legionella bacteria to disinfect them by August 19. (villagevoice.com)
  • Every site tested must submit a "long-term plan" as to how it will guard against the growth of Legionella bacteria. (villagevoice.com)
  • In contrast, a well-established biofilm consists of bacteria clustered together in microcolonies, with surrounding regions of exopolysaccharide and open channels of water that allow food to easily reach the bacteria and waste material to easily pass out of the biofilm. (encyclopedia.com)
  • Bacteria of the genus Legionella h. (bio-medicine.org)
  • Bacteria of the genus Legionella have evolved a sophisticated s. (bio-medicine.org)
  • Bacteria of the genus Legionella have evolved a sophisticated system to replicate in the phagocytic cells of their hosts. (bio-medicine.org)
  • The greatest risk of human infection arises when the bacteria colonize air-conditioning ducts or piping used to transport warm water. (bio-medicine.org)
  • But Legionella turns the tables, resists degradation and continues to grow in the amoeba until it is so full of bacteria that it bursts. (bio-medicine.org)
  • Pathogenic bacteria luxuriate in water with a slightly basic pH, like water found in Klamath Lake. (rense.com)
  • The bacteria are chlorine tolerant 4 and survive the water-treatment process. (cmaj.ca)
  • Osawa K, Shigemura K, Abe Y, Jikimoto T, Yoshida H, Fujisawa M, Arakawa S. A case of nosocomial Legionella pneumonia associated with a contaminated hospital cooling tower. (aaem.pl)
  • Hospital acquired pneumonia: comparison of culture and real-time PCR assays for detection of Legionella pneumophila from respiratory specimens at Tehran Hospitals. (aaem.pl)
  • Today, L. pneumophila is recognized as a common cause of both community-acquired and nosocomial pneumonia ( 84 ). (asm.org)
  • Legionella pneumophila is a waterborne organism that has been increasingly recognized as the causative agent behind community-acquired and nosocomial pneumonia in humans. (jacobspublishers.com)
  • Water was also proved to contain Pseudomonas aeruginonsa, the main GNB-NE causing nosocomila pneumonia at Tehran hospitals . (bvsalud.org)
  • Legionella pneumophila is a cause of pneumonia in human beings. (bvsalud.org)
  • Ιntroducing an effective diagnostic test for L. pneumophila serogroups 2 to 14 in urine and promoting the examination of respiratory specimens from patients hospitalized for pneumonia in Greek hospitals are essential. (cdc.gov)
  • L. pneumophila infection in humans is characterized by a subtle initial immune response, giving time for the organism to establish infection before the patient succumbs to pneumonia. (frontiersin.org)
  • Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. (abcam.com)
  • Legionella generally infects the lung, causing pneumonia, which is often very severe. (sciencephoto.com)
  • The actual number of cases is difficult to detect since all persons with Legionella pneumonia may not have the relevant test for Legionnaire's disease, especially if they have mild symptoms. (news-medical.net)
  • L. pneumophila is the cause of 1-2% of all pneumonia cases in adults(7). (msdsonline.com)
  • Atypical organisms such as Mycoplasma pneumoniae, Chlamydia pneumoniae , and Legionella pneumophila are implicated in up to 40 percent of cases of community-acquired pneumonia. (aafp.org)
  • Legionella and mycoplasma pneumonia-a community hospital experience with atypical pneumonias. (aafp.org)
  • Community-acquired pneumonia is a lung infection that occurs in noninstitutionalized people, typically involving organisms such as viruses, Streptococcus pneumoniae , Klebsiella pneumoniae , Mycoplasma pneumoniae , Legionella pneumophila , Chlamydia pneumoniae , Moraxella species, or Pneumocystis carinii . (thefreedictionary.com)
  • Since the discovery of Legionella pneumophila in 1976 following an outbreak of pneumonia among delegates attending an American Legion convention in Philadelphia, the family Legionellaceae has expanded to include 42 described species, 18 of which have been documented to cause human infection. (cmaj.ca)
  • As many as 15% of North American patients hospitalized because of community-acquired pneumonia may be victims of Legionella spp. (cmaj.ca)
  • Assessment of relative potential for Legionella species or surrogates inhalation exposure from common water uses. (aaem.pl)
  • Among the 48 species of the genus Legionella ( 1 , 4 , 21 , 22 ), L. pneumophila is responsible for approximately 91% of all reported community-acquired cases of legionellosis. (asm.org)
  • The genus Legionella comprises more than 40 species and 64 serogroups with approximately half of those species associated with human diseases. (scielo.org.za)
  • The genus Legionella comprises more than 40 species and 64 serogroups (O'Connell et al. (scielo.org.za)
  • Alternative fluorescence in situ hybridization (FISH) protocols have been proposed, but they result in faint fluorescence signals and lack specificity because of cross-hybridization with other Legionella species. (wiley.com)
  • Isolation of an amoeba naturally harboring a distinctive Legionella species. (microbiologyresearch.org)
  • Surface translocation was also exhibited by some, but not all, other Legionella species examined. (asm.org)
  • Legionella pneumophila is a gram-negative bacterial species that is ubiquitous in almost any aqueous environment. (frontiersin.org)
  • Annotations Using Subsystem Technology server ( 4 ) and Legionella species assignments were determined via BLASTn of the sequence against the NCBI nucleotide database via the web server. (cdc.gov)
  • subsequently the formation of mono and dual-species biofilms by L. pneumophila or H. pylori with the same microorganisms was investigated. (biomedcentral.com)
  • The rest of the cases are caused by other Legionella species. (medlineplus.gov)
  • In addition to colonizing certain mono-species biofilms, L. pneumophila 130 b persisted within a two-species biofilm formed by K. pneumoniae and Flavobacterium sp. (nih.gov)
  • Interestingly, the legionellae were also able to colonize a two-species biofilm formed by K. pneumoniae and P. aeruginosa, demonstrating that a species that is permissive for L. pneumophila can override the inhibitory effect(s) of a non-permissive species. (nih.gov)
  • Presumptive colonies of Legionella species are subcultured in order to provide growth for confirmatory tests. (bruker.com)
  • Although primary culture for Legionella species still requires 10 days of incubation, the MALDI-Biotyper offers same-day confirmation once suspect colonies have been observed. (bruker.com)
  • However it is difficult to discriminate between the L. pneumophila and non- L. pneumophila species rapidly. (springer.com)
  • LS-LAMP amplifies the fragment of the 16S rRNA gene to detect all species of Legionella genus. (springer.com)
  • Cloud JL, Carroll KC, Pixton P, Erali M, Hillyard DR (2000) Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation. (springer.com)
  • Diogo A, Verissimo A, Nobre MF, da Costa MS (1999) Usefulness of fatty acid composition for differentiation of Legionella species. (springer.com)
  • Gobin I, Newton PR, Hartland EL, Newton HJ (2009) Infections caused by nonpneumophila species of Legionella . (springer.com)
  • Most cases are caused by L. pneumophila , however, there may be other causative species of the organism that may lead to milder disease conditions. (news-medical.net)
  • Other than L. pneumophila , 34 species of legionella have been identified. (news-medical.net)
  • The bacterial species are found in natural water supplies and soil. (news-medical.net)
  • Although up to 85-90% of cases can be attributed to L. pneumophila , approximately half of the remaining 49 species in the genus have caused disease in humans(6). (msdsonline.com)
  • While L. pneumophila causes most causes of Legionnaire's Disease, there are a legion of other Legionella species that can cause the disease such as L. longbeachae , L. feeleii , L. micdadei , and L. anisa . (forbes.com)
  • The purpose of the present study was to investigate Acanthamoeba species prevalence in household and hospital potable water in Beni-Suef governorate, Egypt, and to employ sequencing methods to identify positive Acanthamoeba species isolates and their potential health risks. (degruyter.com)
  • The presence of a higher percentage of Acanthamoeba species in tap water, in particular T4, highlights the potential health hazards for immunocompromised individuals and emphasizes the urgent need for the implementation of effective filtration and disinfection measures. (degruyter.com)
  • The Legionella species of gram-negative aerobic bacilli are widely distributed in aquatic habitats and soil. (cmaj.ca)
  • In order to assess the potential risk for legionellosis, we analyzed Legionella contamination of water distribution systems in 36 recreational facilities equipped with swimming pools. (environmental-expert.com)
  • The purpose of this study was to investigate the susceptibility of waterborne strains of Legionella to eight antimicrobials commonly used in legionellosis therapy. (environmental-expert.com)
  • Biofilm detachment and the interaction of Legionella with acanthamoebae were two important ecological factors that significantly increased the risk of legionellosis, and thus should be further considered in the refinement of QMRA models. (iwaponline.com)
  • This fact has resulted in a number of guidelines and control strategies aimed at reducing the risk of legionellosis in building water systems (Diereden, 2008). (scielo.org.za)
  • Isolation of protozoa from water associated with a legionellosis outbreak and demonstration of intracellular multiplication of Legionella pneumophila. (microbiologyresearch.org)
  • How does Legionella pneumophila, the bacterium causing legionellosis, use the host cell machinery to its own advantage? (pasteur.fr)
  • Reliable and optimized technology for specific detection of the causative agents of legionellosis in water samples. (qiagen.com)
  • Critical environments, including water systems in recreational settings, represent an important source of Legionella pneumophila infection in humans. (environmental-expert.com)
  • In manmade water systems, proliferation of L. pneumophila can lead to legionella infection in humans. (iwaponline.com)
  • In contrast, in healthcare settings, the ways we use water are more varied and patients might be more vulnerable to infection. (cdc.gov)
  • Programs such as Developing a Water Management Program to Reduce Legionella Growth and Spread in Buildings: A Practical Guide to Implementing Industry Standards 13.2 are designed, implemented, and regularly reviewed by multidisciplinary teams that include facility managers, infection prevention professionals, clinicians, and administrators. (cdc.gov)
  • Infection occurs after the inhalation of Legionella -contaminated water droplets originating from a wide variety of aerosol-generating devices ( 39 ). (asm.org)
  • Importantly, inhalation of such aerosols is the primary route of infection, rather than ingestion, as is the emphasis of drinking water regulations [ 12 ]. (biomedcentral.com)
  • Molecular-based typing methods have often been used to characterize clinical and environmental Legionella pneumophila to determine the source of infection [5,6,7]. (jacobspublishers.com)
  • In people, infection is thought to occur by inhalation of contaminated water droplets. (frontiersin.org)
  • Recommendations of CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC), Emergency Water Supply Planning Guide for Hospitals and Healthcare Facilities (2012) pdf icon [PDF - 2.07 MB] and standards published by the Association for the Advancement of Medical Instrumentation (AAMI) external icon ), United States Pharmaocopeia (USP), and manufacturers' instructions. (cdc.gov)
  • Hot water from shower heads could be a potential source of infection for Legionella pneumophila . (bvsalud.org)
  • Legionella pneumophila , the causative agent of Legionnaire's disease, replicates in human alveolar macrophages to establish infection. (frontiersin.org)
  • There is no human-to-human transmission and the main source of infection is L. pneumophila biofilms established in air conditioners, water fountains, and hospital equipments. (frontiersin.org)
  • Planktonic L. pneumophila elicits a strong immune response in murine, but not in human macrophages enabling control of the infection. (frontiersin.org)
  • This study advances our understanding of the innate immune response to biofilm-derived L. pneumophila and closely reproduces the natural mode of infection in human. (frontiersin.org)
  • These communities have been identified as a causative source of infection in susceptible hosts who inhale aerosols of contaminated water containing L. pneumophila . (frontiersin.org)
  • Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). (abcam.com)
  • Legionella pneumophila is accounted for more than 80% of Legionella infection. (springer.com)
  • Pontiac fever is a non-pneumonic form of L. pneumophila infection(2). (msdsonline.com)
  • Pontiac Fever is a milder Legionella infection with fever and muscle aches that last no longer than a week as the main symptoms. (forbes.com)
  • Hospital water is an important and controllable source of hospital acquired infection. (clinicaladvisor.com)
  • What are the key methods to minimize transmission of infection to patients by/in water? (clinicaladvisor.com)
  • Clinician epidemiologists must have a high level of suspicion for cases of water borne infections in particular if clusters of infection are identified with signature organisms. (clinicaladvisor.com)
  • Rapid identification of the source of infection is essential to prevent further cases of disease, and a number of conventional PCR assays for the detection of Legionella spp. (asm.org)
  • This bacterial infection is caused primarily by the Gram-negative bacterial genus Legionella . (dovepress.com)
  • This study is designed to determine the contamination degree of hospital water supplies with Pseudomonas aeruginosa, Legionella pneumophila , and E. coli in Gilan, Iran. (hindawi.com)
  • Degrees of contamination with L. pneumophila were 3.3%, 9.3%, and 10.9% and with E. coli were zero, 6.2%, and zero. (hindawi.com)
  • Contamination degree with P. aeruginosa was considerable and with L. pneumophila was moderate. (hindawi.com)
  • The three main forms of water contamination are physical, bacterial, and chemical [ 2 ]. (hindawi.com)
  • Hospital outbreaks are usually associated with contamination of water sources [ 5 ]. (hindawi.com)
  • Water contamination of respirators and incubators of newborns are the main sources of transfer [ 6 , 7 ]. (hindawi.com)
  • In addition, in bacteriological assessments, particularly for coliforms, the indicators of contamination by fecal matter must be routinely performed by many public health authorities to ascertain the quality of water to ensure prevention of further dissemination of pathogens through the agency of water [ 11 ]. (hindawi.com)
  • The aim of this study was to evaluate the potential contamination of Legionella pneumophila (LP) in a large hospital in Italy through georeferential statistical analysis to assess the possible sources of dispersion and, consequently, the risk of exposure for both health care staff and patients. (aaem.pl)
  • The inexpensive, sensitive and rapid real-time PCR based in SYBR Green method is of interest in monitoring Legionella pneumophila contamination, especially in environmental samples, and should be economical for large-scale routine tests. (scielo.org.za)
  • The European Copper Institute (ECI) strongly supports measures that help to improve the quality of water intended for human consumption, and is therefore is in favour of EU-wide standards that help understanding and reduce sources of chemical and /or biological contamination in drinking water systems. (euractiv.com)
  • For advisories without evidence of contamination, tap water may be used for patients with healthy immune systems. (cdc.gov)
  • For advisories with evidence of contamination (microbial/chemical) do not use tap water for bath or shower. (cdc.gov)
  • Conclusions: This study indicates that in spite of using distilled water for incubators, L. pneumophila contamination is considerable and other bacterial contamination is very high. (who.int)
  • Legionella anisa, a possible indicator of water contamination by Legionella pneumophila. (nih.gov)
  • The MALDI Biotyper® system accurately detects cultivated waterborne pathogens within minutes enabling water suppliers to preserve environmental and drinking water from serious contamination events. (bruker.com)
  • Buildings with centralized air conditioning systems particularly large ones like hospitals, hotels, offices, shopping malls etc. may be more prone to legionella contamination because of the complexity of their water systems. (news-medical.net)
  • Systems covered by this standard are not intended for the treatment of water that has obvious contamination or intentional source such as raw sewage, nor are systems intended to convert wastewater to drinking water. (water-research.net)
  • Kim IS, Lee GH, Lee KJ (2000) Monitoring and characterization of bacterial contamination in a high-purity water system used for semiconductor manufacturing. (springer.com)
  • In order to evaluate the usefulness of consumer self-reported data in predicting measured water quality and risk factors for contamination, we compared matched consumer interview data with point-of-survey, household water quality and pressure data for 910 households served by 14 small water systems in rural Alabama. (mdpi.com)
  • We conclude that consumer-reported data were of limited utility in predicting potential microbial risks associated with small water supplies in this setting, although consumer feedback on low pressure-a risk factor for contamination-may be relatively reliable and therefore useful in future monitoring efforts. (mdpi.com)
  • ABSTRACT This study in Iraq investigated the occurrence of Legionella. (who.int)
  • We compared whole-genome sequences of 10 clinical Legionella pneumophila isolates submitted to a laboratory in Genesee County during the second outbreak with 103 water isolates collected the following year. (cdc.gov)
  • We documented a genetically diverse range of L. pneumophila strains across clinical and water isolates. (cdc.gov)
  • Currently, multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) of L. pneumophila isolates can be used as a tool for high-resolution genotyping. (asm.org)
  • MLVA typing is used to determine the allele-related repeat size variation on different VNTR loci of L. pneumophila isolates. (asm.org)
  • pneumophila in different drinking-water sources in Basra governorate as well as the susceptibility of isolates to several antibiotics. (who.int)
  • Environmental testing revealed that a sump pit associated with the air handler was the only source of Legionella in the building and that the strain matched clinical isolates from six case-patients. (cdc.gov)
  • The Legionella pneumophila isolates were grouped into seven distinct Sequence Types, including one (ST1719) which were new to the SBT database of ESGLI and two (ST1223 and ST1718) which were recently also isolated from cooling towers in Kuwait but not have been reported from other countries. (jacobspublishers.com)
  • In this study, we investigated SBT as a molecular tool to characterize 30 Legionella pneumophila isolates from domestic water systems in Kuwait. (jacobspublishers.com)
  • A total number of 30 environmental isolates of L. pneumophila were used in this study. (jacobspublishers.com)
  • These isolates have previously been isolated from domestic water system samples obtained from the faucets of wash basins and showerheads in bathrooms, taps from kitchens and cold and/or hot water tanks from seventeen different residential sites in Kuwait (Figure. (jacobspublishers.com)
  • L. pneumophila isolates were genotyped using the seven gene protocol sequence-based typing (SBT) scheme developed by ESGLI as previously described [5,8]. (jacobspublishers.com)
  • Molecular typing of Legionella pneumophila isolates from environmental water samples and clinical samples using a five-gene sequence typing and sta. (cdc.gov)
  • Inadequate discriminatory power to distinguish between L. pneumophila isolates, especially those belonging to disease-related prevalent sequence types (STs) such as ST1, ST36 and ST47, is an issue of SBT scheme. (cdc.gov)
  • In this study, we developed a multilocus sequence typing (MLST) scheme based on two non-virulence loci (trpA, cca) and three virulence loci (icmK, lspE, lssD), to genotype 110 L. pneumophila isolates from various natural and artificial water sources in Guangdong province of China, and compared with the SBT. (cdc.gov)
  • More intragenic recombinations were detected in nSTs than in STs, and they were both more abundant in natural water isolates. (cdc.gov)
  • In this study, the dispersion, clonality, and virulence of environmental isolates of Legionella pneumophila serogroups 2 to 14 (Lp2-14) in Greece were investigated. (cdc.gov)
  • At a hospital in Halifax, Nova Scotia, Canada, three strains of Legionella pneumophila were detectable based on plasmid content, while the isolates collected at another hospital in Halifax had no plasmids. (asm.org)
  • The potable water at both of these hospitals remained positive for Legionella from 1987 until the present for hospital A and until its demolition in 1996 for hospital B (where plasmidless isolates were obtained). (asm.org)
  • Our objectives in this study were to determine if the genomic DNA of the plasmidless isolates was identical to that of the plasmid-containing isolates, to determine the relatedness of the Legionella population at each institution, and to determine the genomic stability of isolates obtained by longitudinal sampling from the potable water supply. (asm.org)
  • They occur in bulk water and biofilms, where they replicate within protozoa, which can serve as a transmission vehicle and as a protective shell against disinfection or heat treatment ( 2 , 6 , 7 ). (asm.org)
  • Both biofilms and a thermophilic isolate of acanthamoebae were shown to increase the resistance of Legionella to conventional thermal disinfection by between one and two logs respectively. (iwaponline.com)
  • Because of biofilms, L. pneumophila can persist in potable water systems and survive for a long time. (iwaponline.com)
  • Biofilms are common in water supply systems, especially at dead points of old pipes. (iwaponline.com)
  • Therefore, the prevention of biofilms occupies an important role in the elimination of L. pneumophila . (iwaponline.com)
  • Within its aquatic habitats, L. pneumophila survives over a wide temperature range and grows on surfaces, in biofilms, and as an intracellular parasite of protozoa ( 9 , 39 , 110 ). (asm.org)
  • Because L. pneumophila has evolved in a variety of niches, including aquatic environments, biofilms as well as within diverse hosts, different stress response pathways and virulence pathways must be correctly regulated. (frontiersin.org)
  • This work aims to study the influence of five microorganisms isolated from drinking water biofilms on the survival and integration of both of these pathogens into biofilms. (biomedcentral.com)
  • Legionella pneumophila persists within biofilms formed by Klebsiella pneumoniae, Flavobacterium sp. (nih.gov)
  • In aquatic systems, L. pneumophila survives much of time within multi-organismal biofilms. (nih.gov)
  • The levels of L. pneumophila within these biofilms were as much as 4 × 10(4) CFU per cm(2) of steel coupon and lasted for at least 12 days. (nih.gov)
  • and P. fluorescens can promote the presence of L. pneumophila in dynamic biofilms. (nih.gov)
  • To determine the composition of showerhead biofilms and waters, we analyzed rRNA gene sequences from 45 showerhead sites around the United States. (pnas.org)
  • Previous microbiological studies of showerhead biofilms have used culture methodology to detect and identify microbes, and have focused primarily on Legionella pneumophilia ( 7 , 8 , 9 ) and Mycobacterium avium ( 10 - 12 ). (pnas.org)
  • This further leads to an increased surface area between tap water and pipe-wall biofilms, and, hence, higher apparent reaction rates. (nature.com)
  • The control of biofilms is critical for the prevention and control of waterborne pathogens in the hospital water supply. (clinicaladvisor.com)
  • Legionella pneumophila is a gram-negative bacterium which normally exists in water, in soil, and within free-living unicellular protozoa, yet it has developed unique strategies that permit multiplication within the phagosomes of human macrophages. (asm.org)
  • This emerging disease is caused by Legionella pneumophila, an environmental bacterium that can grow in hot water systems. (pasteur.fr)
  • Legionella pneumophila is an intracellular bacterium that has a very sophisticated system to exploit the host cell functions to its own advantage. (pasteur.fr)
  • The collaborative research of scientists belonging to different international teams has revealed that the bacterium Legionella pneumophila (a prokaryote) has acquired a eukaryotic gene that is coding for a major player in lipid metabolism in human cell membranes. (pasteur.fr)
  • Legionella pneumophila ( L. pneumophila ) is a Gram negative facultative bacterium with fastidious growth requirements. (frontiersin.org)
  • Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. (abcam.com)
  • CHARACTERISTICS: Legionella pneumophila is a gram negative, strictly aerobic bacterium of the Legionellaceae family(3). (msdsonline.com)
  • P. Dennis, "Isolation of Legionella from environmental specimens," in A Laboratory Manual for Legionella , T. G. Harrison and A. G. Taylor, Eds. (hindawi.com)
  • The study aims to carry out a three-year retrospective review of routine Legionella follow-up analyses in various samples taken mostly from hospitals in the province of Erzurum and nearby provinces, and to discover a the frequency of isolation of the agent and serogroups from each kind of media. (aaem.pl)
  • Since L. pneumophila is difficult to isolate, the isolation of outbreak strains often fails due to a viable but nonculturable (VBNC) state of the respective environmental population. (asm.org)
  • We were able to detect more L. pneumophila MLVA genotypes in drinking water than we could detect by isolation. (asm.org)
  • L. pneumophila colonies were streaked to isolation to obtain pure cultures. (cdc.gov)
  • We describe the isolation of several Legionella genes essential for intracellular growth and evasion of the endocytic pathway, using a genetic and cell biological approach. (asm.org)
  • Isolation of Legionella spp. (dovepress.com)
  • Sixty-three environmental water samples from various sources were examined for the presence of Legionella pneumophila with a commercially available direct fluorescent monoclonal antibody (GS), an indirect fluorescent antibody test (IFAT) and culture. (nih.gov)
  • The presence of Legionella pneumophila was confirmed by real time PCR assay using primers- probe designed for the mip gene . (bvsalud.org)
  • The iso-estimation maps show clearly the most contaminated pipe and the difference in the diffusion of the different L. pneumophila serogroups. (aaem.pl)
  • The aim of this study is to explore the dispersion, clonality, and virulence of Legionella pneumophila serogroups 2 to 14 in the Greek environment. (cdc.gov)
  • This study showed that there is genetic variability of L. pneumophila serogroups 2 to 14 in the Greek environment as well as a high percentage of the pathogenicity loci. (cdc.gov)
  • This paper describes the fundamental characteristics of point sourced vs. potable water sourced outbreaks and discusses the Bronx outbreak from those perspectives. (environmental-expert.com)
  • Furthermore, during the second outbreak, Legionella spp. (cdc.gov)
  • Erdogan H, Arslan H. Evaluation of a Legionella outbreak emerged in a recently opening hotel. (aaem.pl)
  • This table provides a description of a selected outbreak presented in Outbreaks associated with environmental and undetermined water exposures - United States, 2013-2014 (MMWR Weekly). (cdc.gov)
  • While the outbreak exposure was determined to involve recreational water activities, it could not be classified more specifically as a treated or untreated recreational water exposure. (cdc.gov)
  • Quebec City suffered from a major Legionella outbreak in the summer 2012, resulting in 183 cases and 13 deaths. (mcgill.ca)
  • High-throughput typing method to identify a non-outbreak-involved Legionella pneumophila strain colonizing the entire water supply system in the town of Rennes, France. (helmholtz-hzi.de)
  • Conversely, levels of the mip gene, which is specific to L. pneumophila , were largely below detection in Flint single-family residences, at least during the later stages of the water crisis when they were measured (2015-2016) ( 8 ). (cdc.gov)
  • Parallel to the high-resolution analysis, we used the same environmental DNA to quantify the number of L. pneumophila cells in drinking water using real-time PCR with 16S rRNA gene-targeted primers. (asm.org)
  • Here, we utilize simulated household water heaters to examine the effects of stepwise increases in temperature (32-53 °C), pipe material (copper vs. cross-linked polyethylene (PEX)), and influent assimilable organic carbon (0-700 μg/L) on opportunistic pathogen gene copy numbers and the microbiota composition, as determined by quantitative polymerase chain reaction and 16S rRNA gene amplicon sequencing. (biomedcentral.com)
  • L. pneumophila peaked at 41 °C in the presence of PEX (1.58 × 10 5 gene copies/mL). (biomedcentral.com)
  • To investigate regulatory networks in Legionella pneumophila , the gene encoding the homolog of the Escherichia coli stress and stationary-phase sigma factor RpoS was identified by complementation of an E. coli rpoS mutation. (asm.org)
  • An insertion mutation was constructed in the rpoS gene on the chromosome of L. pneumophila , and the ability of this mutant strain to survive various stress conditions was assayed and compared with results for the wild-type strain. (asm.org)
  • We also provide a model for sRNA-mediated control of gene expression that serves as a framework for understanding the regulation of virulence-related properties of L. pneumophila . (frontiersin.org)
  • and L. pneumophila gene markers from these samples have been published previously ( 2 , 3 ). (cdc.gov)
  • This bioinformatics tool facilitates backward compatibility of whole genome sequence typing by ensuring that the momp S allele, of which L. pneumophila can carry multiple non-identical copies, is typed based on the copy of the gene that would be identified by the traditional PCR-based typing scheme. (cdc.gov)
  • IMSEAR at SEARO: Detection of Pathogenic Legionella pneumophila in Tap Water and Water of Newborn Incubators in Hospitals of Guilan Province by PCR of mip Gene. (who.int)
  • Aims: This study is designed to determine the frequency of Legionella pneumophila in cold and warm water as well as water containers of newborn incubators in Guilan province hospitals, Iran, using amplification of the macrophage infectivity protein gene (mip gene) by PCR. (who.int)
  • DNA was extracted by using the boiling method and PCR was performed to search Legionella and mip gene using two pairs of primers. (who.int)
  • One third of the incubator and one half of the hot water habited L. pneumophila had the mip gene but it was not found in cold tap water samples. (who.int)
  • A specific sequence appears at the 16S rRNA gene of L. pneumophila , while non- L. pneumophila strains have a variable sequence in this site, which can be recognized by the primer of LP-LAMP. (springer.com)
  • X72354 L.pneumophila Sgp.1 (ATCC 33152) gene for 5S ribosomal RNA. (atcc.org)
  • U68079 Legionella pneumophila coded portion of proteolysis tag tmRNA gene. (atcc.org)
  • AF095231 Legionella pneumophila strain ATCC 33152 defect in organelle trafficking protein (dotA) gene, partial cds. (atcc.org)
  • Several bacterial gene products that are essential for intracellular growth of L. pneumophila have been described. (asm.org)
  • Consequently, we used culture-independent methodology based on ribosomal RNA gene sequences to identify the composition of assemblages of microbes associated with showerhead surfaces over a wide geographical area of the U.S. Many of these microbes are closely related to organisms common in water, but some microbes of potential public health concern are enriched to high levels by the showerhead environment. (pnas.org)
  • This kit contains primers for amplification of Legionella pneumophila mip gene, with reagents sufficient to treat 80 bacterial cultures with PMA and perform 200 PCR reactions. (biotium.com)
  • Primers mip-Lpn0901F (5′-AACCGATGACACATCATTA) and mip-Lpn1011R (5′-CTTGCATGACTTTAGCCA) were designed to amplify a 131-bp region at the 5′ end of the macrophage infectivity potentiator ( mip ) gene of L. pneumophila ( 3 ). (asm.org)
  • Then, PCR and LAMP assays were performed for detection of L. pneumophila via its mip gene in respiratory specimens. (dovepress.com)
  • and 3% and 7% of samples were positive for L. pneumophila using the mip gene on PCR and LAMP assays, respectively. (dovepress.com)
  • Aerosols containing Legionella pneumophila generated by shower heads and hot-water faucets. (asm.org)
  • The pathogen is transmitted via small droplets of water, e.g., aerosols from cooling towers, showerheads, or air conditioners. (asm.org)
  • Legionellae are nearly exclusively conveyed via contaminated aerosols as they are produced by showers, whirl pools, air conditioners or humidifiers. (wiley.com)
  • Can be transmitted through aerosols and aspiration of contaminated water. (montana.edu)
  • L. pneumophila is a ubiquitous habitant of natural and human-made water systems and is transmitted to human by inhalation of contaminated aerosols, generated by cooling towers, showers and other devices. (mcgill.ca)
  • Showers are an important interface for human interaction with microbes through inhalation of aerosols, and showerhead waters have been implicated in disease. (pnas.org)
  • MODE OF TRANSMISSION: Can be transmitted by aerosols and aspiration of contaminated water(1,3,4). (msdsonline.com)
  • The disease can be acquired by inhaling aerosols or by microaspiration of water contaminated with Legionella spp. (cmaj.ca)
  • New sequence types from this study submitted to European Working Group for Legionella Infections database ( http://www.ewgli.org ). (cdc.gov)
  • The hospital water supply as a source of nosocomial infections: a plea for action. (aaem.pl)
  • Vural T. Legionella Infections. (aaem.pl)
  • Central to the understanding of infections by the waterborne pathogen Legionella pneumophila is its detection at the clonal level. (asm.org)
  • Epidemiological analyses of infections caused by L. pneumophila depend on the accurate identification of strains, preferably at the clonal level. (asm.org)
  • Open cooling towers are frequent sources of infections with Legionella pneumophila . (wiley.com)
  • However, most Legionella infections are community-acquired without identification of the source ( Pedro-Botet and Sabria 2005 ). (wiley.com)
  • Water can harbor germs that threaten the safety of patients and spread antibiotic resistant pathogens or healthcare-associated infections (HAIs). (cdc.gov)
  • sg10 = 2) were genotyped by the SBT method according to the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group for Legionella Infections (ESGLI formerly EWGLI). (jacobspublishers.com)
  • STs were identified from allele profiles using the European Working Group for Legionella Infections database for L. pneumophila . (cdc.gov)
  • Legionella infections. (medlineplus.gov)
  • Public health authorities downplay the significance of Legionella infections because most originate from drinking water. (theepochtimes.com)
  • These organisms commonly occur in municipal waters and several studies have traced both L. pneumophilia and M. avium infections in hospitalized patients to microbes in their home showers ( 9 , 10 , 12 ). (pnas.org)
  • Health officials realized in retrospect that previous outbreaks of Pontiac Fever, which has nothing to do with driving the car, were in actuality outbreaks of Legionella infections. (forbes.com)
  • Although it is well established that water plumbing systems and faucets are reservoirs are for pathogens, especially Legionella and P. aeruginosa , an international consensus for the prevention and control of water acquired hospital infections has yet to be developed. (clinicaladvisor.com)
  • There is adverse association between bacterial count of water samples and free residual chlorine but the failure of conventional treatment processes to eliminate critical waterborne pathogens in drinking water demands that improved and/or new disinfection technologies should be developed [ 4 ]. (hindawi.com)
  • Notwithstanding this, the current study provided comparative information on the efficacy of conventional disinfection against Legionella . (iwaponline.com)
  • As a consequence of favourable temperatures, and lack of or poor disinfection, levels of legionellae may be of concern in these systems. (scielo.org.za)
  • On the "upstream" side, system disruptions or pressure drops, loss of disinfection residual (e.g. low chlorine levels), and main breaks are all factors that can impact the quality of water within a healthcare system. (cdc.gov)
  • Disinfection of Legionella pneumophila by photocatalytic oxidation. (semanticscholar.org)
  • In the US, it was used for drinking water disinfection in the early 1900s but was abandoned due to high operating costs, unreliable equipment, and the expanding popularity of disinfection by chlorination. (water-research.net)
  • The 'Class B' or similar non-rated UV systems are not intended for the disinfection of 'microbiologically unsafe water. (water-research.net)
  • However, for UV disinfection of drinking water at the household level, the low-pressure lamps and systems are entirely adequate and even preferred to medium pressure lamps and systems. (water-research.net)
  • A crucial point is that effective disinfection systems must be in operation at the onset of water flow into the hospital plumbing system. (clinicaladvisor.com)
  • G. Segal, J. J. Russo, and H. A. Shuman, "Relationships between a new type IV secretion system and the icm/dot virulence system of Legionella pneumophila ," Molecular Microbiology , vol. 34, no. 4, pp. 799-809, 1999. (hindawi.com)
  • This is also the case in L. pneumophila where three sRNAs (RsmY, RsmZ, and 6S RNA) were recently shown to be important determinants of virulence regulation and 79 actively transcribed sRNAs were identified. (frontiersin.org)
  • This suggests that one virulence strategy of Legionella pneumophila is to mimic the functions of human proteins. (pasteur.fr)
  • Swanson is interested in the metabolic cues that underpin the virulence of L. pneumophila and how it is transmitted to humans. (wikipedia.org)
  • Copper pipes supported less L. pneumophila than PEX pipes (mean 2.5 log 10 lower) at temperatures ≤ 41 °C, but showed no difference in total bacterial numbers. (biomedcentral.com)
  • At temperatures ≤ 45 °C, influent assimilable organic carbon correlated well with total bacterial numbers, but not with L. pneumophila numbers. (biomedcentral.com)
  • We subsequently explored relationships among L. pneumophila and other ecologically relevant microbes, noting that elevated temperature did not have a general disinfecting effect in terms of total bacterial numbers. (vt.edu)
  • Members of the Faucher lab have a focused interest on the behaviour of bacterial pathogens in non-clinical environments, such as in water. (mcgill.ca)
  • Here, we track tap water microbiome assembly in situ, showing that bacterial community composition changes rapidly from the city supply following ~6-day stagnation, along with an increase in cell count from 10 3 cells/mL to upwards of 7.8 × 10 5 cells/mL. (nature.com)
  • A potential link has been reported between bacterial community composition and occurrences of opportunistic pathogens [ 10 ], suggesting the microbiome could be a prospective indicator to aid water quality surveillance and management. (nature.com)
  • Tracking stagnation effects on the bacterial communities in tap water can help to elucidate how tap water deviates from the city supply and to understand what magnitude of deviation is permissible. (nature.com)
  • Biofilm formation is a key point in the spread and transmission to humans of L. pneumophila . (iwaponline.com)
  • The hypothesis upon which this study was based was that biofilm matrices, known to provide a habitat and a gradient of nutrients, might allow the survival and multiplication of legionellae outside a host cell. (microbiologyresearch.org)
  • The levels of L. pneumophila cells present in the biofilm were monitored for 15 d, with and without the presence of H. vermiformis , and it was found that, although unable to replicate in the absence of H. vermiformis , L. pneumophila was able to persist. (microbiologyresearch.org)
  • For example, at 51 °C, planktonic L. pneumophila in recirculating lines was reduced by a factor of 28.7 compared to 39 °C and was prevented from re-colonizing biofilm. (vt.edu)
  • For biofilm studies, sessile cells were quantified in terms of total cells by SYTO 9 staining, viable L. pneumophila or H. pylori cells were quantified using 16 S rRNA-specific peptide nucleic acid (PNA) probes and cultivable cells by standard culture techniques. (biomedcentral.com)
  • One type of biofilm that has been widely studied is that formed in drinking water distribution systems (DWDS) because of its role in the persistence of pathogens in drinking water and the consequent potential for impact on public health [ 10 - 12 ]. (biomedcentral.com)
  • It may be related to the length of time that water remains in an incubator container which is a predisposing factor for both biofilm formation and the growth of water microflora. (who.int)
  • Interactions between planktonic L. pneumophila and murine or human macrophages have been studied for years, yet the interface between biofilm-derived L. pneumophila and macrophages has not been explored. (frontiersin.org)
  • In contrast to planktonic L. pneumophila , biofilm-derived L. pneumophila lacks flagellin expression, do not activate caspase-1 or -7 and trigger less cell death. (frontiersin.org)
  • L. pneumophila was able to intercalate into and persist within a biofilm formed by Klebsiella pneumoniae, Flavobacterium sp. (nih.gov)
  • The "starvation-survival" mechanisms, including biofilm formation, may be associated with deleterious effects on industrial water systems. (springer.com)
  • Sarro MI, Garcia AM, Moreno DA (2005) Biofilm formation in spent nuclear fuel pools and bioremediation of radioactive water. (springer.com)
  • Shower heads and hot-water faucets containing Legionella pneumophila were evaluated for aerosolization of the organism with a multistage cascade impaction air sampler. (asm.org)
  • Shower heads and hot-water taps containing L. pneumophila can aerosolize low numbers of the organism during routine use. (asm.org)
  • Treatment of two infected towers with a chlorinated phenol (100 p.p.m.) eliminated legionellae for at least three days, but after 14 days the organism was again found. (nih.gov)
  • The causative organism was Legionella pneumophila . (news-medical.net)
  • Proper decontamination of containers used to store and dispense water is crucial for preventing illnesses and outbreaks caused by Pseudomonas and other waterborne disease pathogens. (cdc.gov)
  • The IDEXX Pseudalert method has been published as the International Organization for Standardization (ISO) worldwide standard 16266-2 for the 24-hour detection of Pseudomonas aeruginosa in water. (idexx.com)
  • Listed under ISO 16266-2:2018, Water Quality-Detection and Enumeration of Pseudomonas aeruginosa-Part 2: Most Probable Number Method , the Pseudalert method detects P. aeruginosa in 24 hours, less than half the time of other commercially available methods. (idexx.com)
  • Colonization of hospital water systems by Legionella pneumophila, Pseudomonas aeroginosa, and Acinetobacter in ICU wards of Tehran hospitals. (bvsalud.org)
  • Legionella pneumophila , Pseudomonas aeroginosa and Acinetobacter were isolated from 5 (9.6%), 6 (11.4%) and 1 (1.8%) of the hospital water systems, respectively. (bvsalud.org)
  • This study demonstrated the presence of Legionella , Pseudomonas and Acinetobacter in water system in ICU wards of different hospitals in Tehran. (bvsalud.org)
  • So, drinking water distribution systems may contain a diverse microbiological community that may include opportunistic pathogens and it is the most important single source of human exposure to gastroenteric diseases, mainly as a result of the ingestion of microbial contaminated water [ 4 ]. (hindawi.com)
  • Falkinham JO, Pruden A, Edwards M. Opportunistic premise plumbing pathogens: Increasingly important pathogens in drinking water. (aaem.pl)
  • These findings provide guidance among multiple control measures for the growth of opportunistic pathogens in hot water plumbing and insight into the mediating role of microbial ecological factors. (biomedcentral.com)
  • Preventing proliferation of opportunistic pathogens (OPs) in building plumbing is an important step in delivery of safe drinking water to consumers. (biomedcentral.com)
  • For this reason, the Centers for Medicare & Medicaid Services (CMS) and CDC consider it essential that hospitals and nursing homes have a water management program that is effective in limiting Legionella and other opportunistic pathogens of premise plumbing from growing and spreading in their facility. (cdc.gov)
  • Hot water systems are especially vulnerable to the growth of opportunistic pathogens (OPs), such as Legionella pneumophila and Mycobacteria avium . (biomedcentral.com)
  • yet, there are discrepancies in past literature regarding the effects of water heater temperature and water use patterns on the occurrence of opportunistic pathogens, in particular Legionella pneumophila. (vt.edu)
  • Hospital washbasin water: risk of Legionella-contaminated aerosol inhalation. (aaem.pl)
  • appeared to have an antagonistic effect on L. pneumophila cultivability but not on the viability (as assessed by rRNA content using the PNA probe), possibly leading to the formation of viable but noncultivable (VBNC) cells, whereas Mycobacterium chelonae increased the cultivability of this pathogen. (biomedcentral.com)
  • Survival and perhaps growth of water-borne pathogen in this environment is crucial for its transmission to its host. (mcgill.ca)
  • Albert-Weissenberger C, Cazalet C, Buchrieser C (2007) Legionella pneumophila -a human pathogen that co-evolved with fresh water protozoa. (springer.com)
  • She has investigated the water-borne pathogen Legionella pneumophila. (wikipedia.org)
  • Day-to-day water stagnation in building plumbing can potentially result in water quality deterioration (e.g., lead release or pathogen proliferation), which is a major public health concern. (nature.com)
  • It appears that M. chelonae may have an important role in the survival of both pathogens in drinking water. (biomedcentral.com)
  • Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems. (helmholtz-hzi.de)
  • Spatial distribution of Legionella pneumophila MLVA-genotypes in a drinking water system. (helmholtz-hzi.de)
  • Legionellae can infect and multiply intracellularly in both human phagocytic cells and protozoa. (microbiologyresearch.org)
  • Growth of legionellae in the absence of protozoa has been documented only on complex laboratory media. (microbiologyresearch.org)
  • These data suggest that L. pneumophila possesses a growth phase-dependent resistance to stress that is independent of RpoS control and that RpoS likely regulates genes that enable it to survive in the environment within protozoa. (asm.org)
  • Legionella pneumophila is commonly found in almost all natural and engineered water systems where it replicates in a variety of phagocytic protozoa, including Hartmannella vermiformis . (frontiersin.org)
  • Αll strains were further analyzed for detection of two pathogenicity loci: Legionella vir homologue (lvh) and repeats in structural toxin (rtxA). (cdc.gov)
  • Legionella pneumophila replicates within a specialized phagosome in cultured cells, a function necessary for its pathogenicity. (asm.org)
  • Applying chemicals to water supply systems may present risks for humans, the environment and the water pipes. (iwaponline.com)
  • Poultry and cattle are carriers of C. jejuni and transmission to humans is associated with consumption of uncooked meat, unpasteurized dairy product and contaminated water. (mcgill.ca)
  • Dr. Faucher is also interested in the effect of environmental conditions and resident microbiome on the transmission of L. pneumophila from water system to humans and on the induction of the viable but non-culturable state (VBNC). (mcgill.ca)
  • In humans Legionella is responsible for the so-called Legionna. (bio-medicine.org)
  • Most Legionella -associated illnesses in humans are caused by Legionella pneumophila . (bio-medicine.org)
  • Our aim was to study the occurrence of L. pneumophila in the water supply system on board Norwegian naval vessels as the basis for framing preventive strategies against Legionella infect. (sintef.no)
  • When L. pneumophila cells infect the human lung, essentially the same thing happens. (bio-medicine.org)
  • In our survey, 103 water samples from 50 cooling towers were examined over a five-year period to indicate the seasonal distribution and the ecology of L. pneumophila, as regards temperature and pH. (environmental-expert.com)
  • The minimum inhibitory concentrations (MICs) of 66 environmental Legionella strains, isolated from fountains and cooling towers of public facilities (hotels, schools, and shopping malls) in Macau and Guangzhou, were tested using the microdilution method in buffered yeast extract broth. (environmental-expert.com)
  • A total of 50 samples from cooling towers and hot tap water systems were analysed by DNA amplification using 2 pairs of primers targeting the mip and dot genes. (scielo.org.za)
  • 1998). They are present in process facilities' water systems such as cooling towers, water networks in hospitals, industrial or residential buildings and hydrothermal devices, among others. (scielo.org.za)
  • concentrations in cooling towers or other water systems. (wiley.com)
  • From these they may enter the artificial water supply systems in the air conditioning, hot and cold water services and cooling towers. (news-medical.net)
  • The mayor's office on Monday announced that by Friday, "all sites will submit long-term plans as to how they will maintain the cooling towers to protect against any future growth of legionella. (villagevoice.com)
  • are found in the environment and can enter man-made hot-water systems, air conditioners, and cooling towers of defined facilities, such as hotels, hospitals, and whirlpool spas. (dovepress.com)
  • Further environmental investigation was unable to identify which water exposure was the source of the Cryptosporidium . (cdc.gov)
  • Addition of chlorine in different water treatment plants is a common practice, but it is not sufficient to ensure the safety of water. (hindawi.com)
  • Treatment of a contaminated system, either with a 10 p.p.m mixture of a quaternary ammonium compound and tributyltinoxide or slow release chlorine briquettes (maximum recorded free chlorine level 1.2 p.p.m.), did not eliminated legionellae. (nih.gov)
  • Water samples were analyzed for free and total chlorine, pH, turbidity, and presence of total coliforms and Escherichia coli . (mdpi.com)
  • L. pneumophila strains in Flint tap water after the outbreaks were diverse and similar to some disease-causing strains. (cdc.gov)
  • and L. pneumophila genes were found to be higher in the tap water of large buildings in Flint than in other water systems in US areas not experiencing outbreaks ( 8 ). (cdc.gov)
  • As early as 1987, hospital hot water tanks with temperature settings below 55 °C were identified as the primary source for nosocomial Legionnaire's disease outbreaks [ 8 , 9 ]. (biomedcentral.com)
  • Conclusions Our study provides a new window of understanding into the microbial ecology of potable hot water systems and helps to resolve past discrepancies in the literature regarding the influence of water temperature and stagnation on L. pneumophila, which is the cause of a growing number of outbreaks. (vt.edu)
  • Nine known L. pneumophila genomes associated with previous LD outbreaks were included in the analysis as reference strains for comparison (Table S2). (cdc.gov)
  • IMPORTANCE L. pneumophila is the leading cause of disease outbreaks associated with drinking water in the United States. (asm.org)
  • Numerous outbreaks have been linked to contaminated water ( Table I ). (clinicaladvisor.com)
  • Other potential sources of legionella include spa and whirlpool baths, humidi﫿ers and 﫿re-﫿ghting systems. (news-medical.net)
  • and L. pneumophila using a quantitative polymerase chain reaction (qPCR). (bsigroup.com)
  • Detection of Acanthamoeba in tap water and contact lens cases using polymerase chain reaction. (degruyter.com)
  • We surveyed 236 potentially pathogenic Acanthamoeba strains, isolated from water sources in the Canary Islands, for the presence of human adenoviruses (HAdV) using a polymerase chain reaction (PCR)-based typing assay. (ajtmh.org)
  • Investigation comments suggest several contributing factors, including pump discharge failure, water temperature reaching prime Legionella growth range, and high levels of humidity resulting in longer suspension of water droplets from the HVAC system in the environment. (cdc.gov)
  • Man-made water systems especially hot water systems are main sources of Legionella [ 9 ]. (hindawi.com)
  • The results suggested that EOs of lemon, peppermint, sage and thyme had the potential for being used in the removal of L. pneumophila from potable water systems, due to their antibacterial and antibiofilm activities. (environmental-expert.com)
  • Water systems of large buildings, such as hospitals, are often contaminated with legionellae and therefore represent a potential risk for the hospital population. (aaem.pl)
  • Legionella positivity was found to be higher in the hot water systems of hospitals and related points, compared to the other sample points. (aaem.pl)
  • Environmental (Saprozoic) Pathogens of engineered water systems: understanding their ecology for risk assessment and management. (aaem.pl)
  • In drinking water supply systems (DWSSs), legionellae can survive in dead-end tubings, stagnated water in plumbings, or seldom-used facilities ( 2 ). (asm.org)
  • Fourteen recirculating cooling water systems were surveyed during the summer, 1981, to see what factors might influence the prevalence of Legionella pneumophila. (nih.gov)
  • L. pneumophila was isolated from six of the 14 cooling systems at the beginning of the trial but by the end was present in ten. (nih.gov)
  • Repeated tests on eight untreated systems showed that two were consistently infected, three became and remained infected, one was infected on a single occasion and two were never infected with L. pneumophila. (nih.gov)
  • Consequently, searching for alternative products to disinfect water systems is attracting more attention. (iwaponline.com)
  • In an attempt to provide a simple screening method for Legionella pneumophila in water systems samples a real time PCR assay using SYBR Green was developed. (scielo.org.za)
  • Air-handling and water systems of buildings - Microbial control - Design, installation and commissio. (standards.govt.nz)
  • Several biotic and abiotic factors have been reported to influence the proliferation of microbes, including Legionella pneumophila , in hot water premise plumbing systems, but their combined effects have not been systematically evaluated. (biomedcentral.com)
  • In considering options for intentionally shaping the microbiota in building plumbing systems, temperature and pipe material are practical factors that can be directly controlled at the building level, while assimilable organic carbon (AOC) is influenced by both municipal water treatment and conditions in the building plumbing. (biomedcentral.com)
  • As the hospitality sector is ready to reopen its doors and welcome back customers, some public health risks may arise if water systems in hotels have not been properly managed during the lockdown, a health expert warned. (euractiv.com)
  • in water systems is prerequisite for reliable risk estimation. (wiley.com)
  • Healthcare facilities can have large complex water systems that promote growth of pathogenic organisms if not properly maintained. (cdc.gov)
  • hospitals and healthcare systems are often among a water utility's largest customers. (cdc.gov)
  • L. pneumophila is a gram-negative, gammaproteobacterium that is widespread in natural and manufactured water systems ( 22 , 39 , 93 ). (asm.org)
  • Turnaround time is especially critical in the healthcare and hospitality industries, where receiving test results sooner enables facility managers to act more quickly to clean their water systems and protect the safety of patients and visitors. (idexx.com)
  • Heat shock is a potential control strategy for Legionella pneumophila in hot water plumbing systems. (biomedcentral.com)
  • Hot water systems are a key source of microbes to the human-occupied built environment and harbor distinct microbiota from that of influent potable water [ 1 ]. (biomedcentral.com)
  • Further, in-building hot water systems often involve storage equipment (e.g., water heater or hot water tank), which can serve as a reservoir for microbes and contribute to downstream warm water conditions conducive to microbial growth [ 6 ]. (biomedcentral.com)
  • Such unique aspects of hot water systems, together with the inherent heterogeneity in domestic plumbing designs across different buildings, make it almost impossible to promote a unified control strategy for microbial regrowth. (biomedcentral.com)
  • This is the first study used to describe the use of SBT to characterize environmental Legionellapneumophila strains isolated from domestic water systems in Kuwait. (jacobspublishers.com)
  • Only two study reports the characterization of Legionella that has been isolated from potable water systems in Kuwait [11,12]. (jacobspublishers.com)
  • Other sources include water samples from water systems and fresh water sources. (montana.edu)
  • In this review we describe current knowledge about sRNAs and their regulatory properties and how this relates to the known regulatory systems of L. pneumophila . (frontiersin.org)
  • BACKGROUND Little is known about the occurrence of Legionella pneumophila in water supply systems on board ships. (sintef.no)
  • Aurell H, Catala P, Farge P, Wallet F, Le Brun M, Helbig JH, Jarraud S, Lebaron P (2004) Rapid detection and enumeration of Legionella pneumophila in hot water systems by solid-phase cytometry. (springer.com)
  • Legionella pneumophila is widespread in natural water sources and often colonize in manufactured water systems. (sciencephoto.com)
  • Swanson has studied the mechanisms that permit L. pneumophila to persist in water, looking to create a microbiology based system to assess the safety of public water systems. (wikipedia.org)
  • Class A - These ultraviolet water treatment systems must have an 'intensity & saturation' rating of at least 40,000 uwsec/cm2 and possess designs that will allow them to disinfect and/or remove microorganisms from contaminated water. (water-research.net)
  • The systems are intended to be installed on visually clear water. (water-research.net)
  • Portable Automated Biosensing of Potential Dual-Use Biothreats to Water Systems. (usherbrooke.ca)
  • There are a number of similarities between the physicochemistry of industrial water systems and some natural aquatic ecosystems, and these similarities extend to the microbial populations and the survival mechanisms that are employed. (springer.com)
  • Biological fouling of industrial water systems has significant direct and indirect (through antimicrobial chemical applications) impacts on engineered materials and on the etiology of some waterborne diseases. (springer.com)
  • This review provides an overview of the microbial ecology of purified waters and discusses the impacts of biological activity on industrial systems. (springer.com)
  • in water-distribution systems in 83%-92% of health care facilities, 5 , 6 raising fears that it may be ubiquitous in hospital plumbing systems. (cmaj.ca)
  • There are 3 effective ways to disinfect water-distribution systems. (cmaj.ca)
  • 2 Recommendations for home water systems are less clear. (cmaj.ca)
  • The Prevalence rat of L. pneumophila was found to be quite low compared to other studies conducted in the western regions of Turkey. (aaem.pl)
  • This identification, in addition to epidemiological studies, point to different prevalence of H. pylori in the microbial population which is associated with the type of source water. (biomedcentral.com)
  • EPIDEMIOLOGY: L. pneumophila is of worldwide prevalence(7). (msdsonline.com)
  • K. H. Berger and R. R. Isberg, "Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila ," Molecular Microbiology , vol. 7, no. 1, pp. 7-19, 1993. (hindawi.com)
  • The molecular ecology of legionellae. (microbiologyresearch.org)
  • DNA was extracted from Legionella cultures by resuspending colonies in 50 l of molecular grade water, freezing at 20°C, and rapidly thawing at 90°C for 10 minutes. (cdc.gov)
  • Using genetic and molecular tools, Dr. Faucher is studying their implication in the ability of L. pneumophila to grow inside host cells. (mcgill.ca)
  • The growth defects of two of these strains were complemented by molecular clones from a bank constructed from a wild-type L. pneumophila strain. (asm.org)
  • Swanson has studied how Legionella pneumophila remains virulent in different environments. (wikipedia.org)
  • Effects of cytochalasin D and methylamine on the intracellular growth of Legionella pneumophila in amoebae and human monocyte-like cells. (microbiologyresearch.org)
  • Six mutant strains of L. pneumophila that had severe intracellular growth defects in mouse bone marrow-derived macrophages were identified. (asm.org)
  • M. A. Söderberg, O. Rossier, and N. P. Cianciotto, "The type II protein secretion system of Legionella pneumophila promotes growth at low temperatures," Journal of Bacteriology , vol. 186, no. 12, pp. 3712-3720, 2004. (hindawi.com)
  • Among the processes that promote L. pneumophila growth in both the environment and the mammalian lung are Lsp type II protein secretion, Dot/Icm type IVB protein secretion, and Lvh type IVA protein secretion ( 5 , 25 , 31 , 106 ). (asm.org)
  • The most prevalent L. pneumophila strains isolated from residential plumbing were closely related strains of SG6. (asm.org)
  • We found that the specificity was 100% for both LS-LAMP and LP-LAMP, and the sensitivity of LAMP assay for L. pneumophila detection was between 52 and 5.2 copies per reaction. (springer.com)
  • The results suggest that real-time LAMP, as a new assay, provides a specific and sensitive method for rapid detection and differentiation of Legionella spp. (springer.com)
  • the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. (asm.org)
  • This paper describes a prototype real-time assay using LightCycler methodology that detects L. pneumophila within 90 min of receipt of water samples. (asm.org)
  • For determining whether this was the result of relapse or reinfection, Legionella pneumophila strains, cultured from sputum specimens and his home water supply, were characterized by pulsed-field gel electrophoresis. (biomedsearch.com)
  • The aim of this study was the detection of L. pneumophila in respiratory specimens of hospitalized patients with respiratory symptoms by culture, PCR, and loop-mediated isothermal amplification (LAMP) methods. (dovepress.com)
  • A. Marra, S. J. Blander, M. A. Horwitz, and H. A. Shuman, "Identification of a Legionella pneumophila locus required for intracellular multiplication in human macrophages," Proceedings of the National Academy of Sciences of the United States of America , vol. 89, no. 20, pp. 9607-9611, 1992. (hindawi.com)
  • In mammals, L. pneumophila replicates inside macrophages within a modified vacuole. (frontiersin.org)
  • Once in the lungs, L. pneumophila infects and replicates inside alveolar macrophages. (frontiersin.org)
  • Legionella sometimes survives as an intracellular parasite of amoebae and ciliates (Ratcliff et al. (scielo.org.za)
  • Some amoebae occurring in drinking water, such as Acanthaomeba spp. (biomedcentral.com)
  • Association between Legionella pneumophila and amoebae in water. (microbiologyresearch.org)
  • Legionella like it wet, and they like amoebae. (forbes.com)
  • Assessment of Two Different Drinking Water Treatment Plants for the Removal of Free-living Amoebae, Egypt. (degruyter.com)
  • Surveillance of potentially pathogenic free-living amoebae through drinking water treatment processes in Fayoum Governorate, Egypt. (degruyter.com)
  • 2014. Presence of potentially pathogenic free-living amoebae strains from well water samples in Guinea-Bissau. (degruyter.com)
  • strain CDC-65 (Δ) (B), P. aeruginosa ATCC strain 7700 (◊) (C), or P. fluorescens ATCC strain 17569 (Δ) (D) pre-formed on stainless steel coupons was exposed to L. pneumophila strain 130b (▪) on day 3 and flow of 1∶100 R2A began at 1-2 ml/min. (nih.gov)
  • M19444 L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 2 of 3. (atcc.org)
  • Legionellae are ubiquitous inhabitants of natural and human-made aquatic environments. (asm.org)
  • Legionellae are ubiquitous in the natural environment, being present in soils and aquatic ecosystems (Fliermans et al. (scielo.org.za)
  • Disease is acquired by inhaling aerosol contaminated by legionellae. (environmental-expert.com)
  • Approximately 90% (7 of 8 CFU) of the L. pneumophila recovered were trapped in aerosol particles between 1 and 5 micron in diameter. (asm.org)
  • Finally, when focusing "downstream," water management programs should consider the various pathways that might lead to patients becoming exposed to waterborne pathogens, in relation to ingestion, diet, hygiene, and clinical care. (cdc.gov)
  • Temperature-Dependent Growth Modeling of Environmental and Clinical Legionella pneumophila Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Genotypes. (helmholtz-hzi.de)
  • Common to one environmental and four clinical samples were strains of L. pneumophila SG1 and ST1, the most prevalent ST worldwide. (asm.org)
  • We also thank Qing-Yi Zhu from the Guangzhou Kingmed Center for Clinical Laboratory for providing the DNA or strains of L. pneumophila and non- L. pneumophila . (springer.com)
  • 1 , 2 Laboratory and clinical data suggest that fluoroquinolone and newer macrolide-azalide agents against L. pneumophila are superior to erythromycin. (cmaj.ca)
  • Z24705 L.pneumophila 23S and 5S rRNA genes, partial CDS's. (atcc.org)
  • AJ011775 Legionella pneumophila djlA and kdtA (waaA) genes. (atcc.org)
  • One hundred and sixty water samples were analyzed from shower heads or taps located in locker rooms or in bathrooms. (environmental-expert.com)
  • Over 50% of the examined samples were positive for Legionella pneumophila . (aaem.pl)
  • The study included a total of 2,025 water samples taken from hospitals, hotels, Turkish baths and shopping malls in Erzurum,and 13 nearby cities between 2016 and 2018. (aaem.pl)
  • Samples were filtered by 0.45 μm-diameter membrane filter paper in the Public Health Laboratory of Erzurum and examined for L. pneumophila using culture method, according to the criteria set out in ISO 11731-2. (aaem.pl)
  • The presence of L. pneumophila was found in 65 of the 2,025 water samples taken in hospitals, and in none of 40 samples taken in hotels, Turkish baths and shopping malls. (aaem.pl)
  • GS detected L. pneumophila in 94% and 100% of environmental water samples which were culture and IFAT positive for L. pneumophila, respectively. (nih.gov)
  • IFAT detected 69% of L. pneumophila culture positive samples. (nih.gov)
  • L. pneumophila in environmental samples was easy to detect against a clear dark background when stained with GS. (nih.gov)
  • We used a set of drinking water samples from a small-scale drinking water network to test our approach. (asm.org)
  • With these samples we demonstrated that the developed approach was directly applicable to DNA obtained from drinking water. (asm.org)
  • and 46 samples from tankers and plants supplying water by reverse osmosis. (who.int)
  • in water samples. (wiley.com)
  • It also has high sensitivity and specificity, requires no confirmation steps, and can be more easily used with water samples that have high levels of background flora. (idexx.com)
  • A total of 52 water samples from shower bath and taps water in seven hospitals of Tehran were collected. (bvsalud.org)
  • Water samples were prepared, concentrated and then cultured on selective [GVPC] media. (bvsalud.org)
  • From 120 water samples 27 samples were contaminated with L.pneumophila. (bvsalud.org)
  • Results: About 8.5% of the samples had L. pneumophila including 11% of the incubators and 5.8% of both hot and cold tap water. (who.int)
  • Following primary culture of water samples, colonies which are suspected of being potential pathogens require confirmatory tests to exclude or verify the presence of specific organisms. (bruker.com)
  • Testing water and environmental samples for microbial pathogens is a matter of public health and safety. (qiagen.com)
  • In the environmental water samples detection, a total of 107 water samples were identified by the method. (springer.com)
  • and L. pneumophila and should be utilized to test environmental water samples for increased rates of detection. (springer.com)
  • Dusserre E, Ginevra C, Hallier-Soulier S, Vandenesch F, Festoc G, Etienne J, Jarraud S, Molmeret M (2008) A PCR-based method for monitoring Legionella pneumophila in water samples detects viable but noncultivable legionellae that can recover their cultivability. (springer.com)
  • In lung or sputum samples, L. pneumophila appears as a small to short rod with a length of 3 to 5 μm. (msdsonline.com)
  • Sixty tap water samples (30 household and 30 governmental and private hospital settings) collected from Beni-Suef governorate, Egypt were filtered, cultured on non-nutrient agar, identified by morphotyping keys after staining with Giemsa stain and then confirmed by PCR using Acanthamoeba specific primers. (degruyter.com)
  • One-liter natural water samples were concentrated ( 4 ) to a final volume of ca. 1 ml, of which 80 μl was added to 100 μl of ATL buffer and processed using the QIAgen kit to a final DNA eluate of 50 μl. (asm.org)
  • Microbial water samples were taken at the point-of-use (from kitchen faucets) and as-delivered from the distribution network (from outside flame-sterilized taps, if available), where pressure was also measured. (mdpi.com)
  • While total coliforms (TC) were detected in 17% of outside tap samples and 12% of samples from kitchen faucets, no reported water service conditions or aesthetic characteristics were associated with presence of TC. (mdpi.com)
  • Our results showed that 1% of the samples were culture positive for Legionella spp. (dovepress.com)
  • The IDEXX Colilert®-18 method was published as an ISO standard (ISO 9308-2) in 2012 and was included as one of the two reference methods for coliforms and E. coli in the revised European Union Drinking Water Directive in 2015. (idexx.com)
  • Our data indicate that the role of rpoS in L. pneumophila is very different from what has previously been reported for E. coli rpoS . (asm.org)
  • Experimental work has demonstrated that geostatistical methods applied to the microbiological analysis of water matrices allows a better modeling of the phenomenon under study, a greater potential for risk management and a greater choice of methods of prevention and environmental recovery to be put in place with respect to the classical statistical analysis. (aaem.pl)
  • Delia S, Laganà P. Environmental research of L. pneumophila at the Polyclinic of Messina. (aaem.pl)
  • This baseline data will form the basis for the development of a Legionella environmental surveillance program to be used for future epidemiological investigations. (jacobspublishers.com)
  • 2) Present address: Institute of Environmental Assessment and Water Research (IDAEACSIC), Barcelona, Spain. (pasteur.fr)
  • We verified by immunostaining that SG1-specific antibody does not cross-react with the SG6 L. pneumophila environmental strains. (asm.org)
  • As a leading provider of PCR-based technology with extensive experience in areas of health and safety, QIAGEN knows what your lab needs for environmental and water testing. (qiagen.com)
  • Infinite Water is committed to working towards a sustainable water future from environmental, social and corporate perspectives. (wioa.org.au)
  • The objective of this study was to determine whether potable water in ICU wards at Tehran hospitals is contaminated with L. pneomophila, P. aeroginosa and Acinetobacter spp. (bvsalud.org)
  • Compared to what is known of the established risks of colonization within hospitals and hotels, relatively little is known about residential exposure to L. pneumophila . (asm.org)
  • In developed countries, the water supply networks in homes, schools, and hospitals (collectively termed as premise plumbing), have been identified as a hotspot for waterborne pathogens [ 1 , 4 ]. (nature.com)
  • from drinking waters in several hospitals of Iran. (degruyter.com)
  • Detection and quantification of Legionella spp. (document-center.com)
  • This document specifies a method for the detection and quantification of Legionella spp. (bsigroup.com)
  • Détection et quantification de Legionella spp. (bsigroup.com)
  • Microorganisms can be transmitted to the host by contaminated items, such as water. (hindawi.com)
  • This work also suggests that the presence of some microorganisms can decrease the cultivability of L. pneumophila but not the viability which indicates that the presence of autochthonous microorganisms can lead to misleading results when the safety of water is assessed by cultivable methods alone. (biomedcentral.com)
  • However, although drinking water is closely monitored for its biological quality based on indicator microorganisms in public supplies, active surveillance of water after entering a building is not included in current regulations [ 7 ]. (nature.com)
  • These microorganisms are found in soil, lakes and rivers, and can enter our water supply via the groundwater. (bio-medicine.org)
  • Can survive up to 139 days in distilled water and 415 days in tap water. (montana.edu)
  • L. pneumophila is able to survive up to a year in water without the presence of any food source, i.e. susceptible protozoan. (mcgill.ca)
  • Legionella can survive in low temperatures, but thrive at temperatures between 20ºC and 45ºC. (news-medical.net)
  • A simplistic quantitative microbial risk assessment (QMRA) based on the maximum risk curve (r = 1) was developed for Legionella within a water distribution system. (iwaponline.com)
  • Quantitative PCR measurements demonstrated that L. pneumophila relative abundance at distal taps also was not significantly different at 2-month post-heat shock relative to the pre-heat shock condition, while relative abundance of Vermamoeba vermiformis , a known Legionella host, did increase. (biomedcentral.com)
  • In the thermal disruption scenario, relative abundance of planktonic L. pneumophila (quantitative PCR data) increased to levels comparable to those observed in the heat shock scenario within 2 months of switching long-term operation at 60 to 40 °C. Overall, water use frequency and water heater temperature set point exhibited a stronger effect than one-time heat shock on the microbial composition and Legionella levels at distal taps. (biomedcentral.com)
  • L. pneumophila is especially challenging to control because it establishes and grows as part of the native plumbing microbiota, even under oligotrophic conditions and in the presence of disinfectants. (biomedcentral.com)
  • SUSCEPTIBILITY TO DISINFECTANTS: L. pneumophila is susceptible to 1% solutions of sodium hypochlorite, 2% phenol, 2% gluteraldehyde, isopropyl alcohol, and formaldehyde(19). (msdsonline.com)