von Willebrand Factor: A high-molecular-weight plasma protein, produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. It functions in adhesion of platelets to collagen and hemostatic plug formation. The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor.Vascular Endothelial Growth Factor A: The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.Vascular Endothelial Growth Factors: A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.von Willebrand Diseases: Group of hemorrhagic disorders in which the VON WILLEBRAND FACTOR is either quantitatively or qualitatively abnormal. They are usually inherited as an autosomal dominant trait though rare kindreds are autosomal recessive. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII, and impaired platelet adhesion.Endothelial Growth Factors: These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.Vascular Endothelial Growth Factor Receptor-2: A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.Receptors, Vascular Endothelial Growth Factor: A family of closely related RECEPTOR PROTEIN-TYROSINE KINASES that bind vascular endothelial growth factors. They share a cluster of seven extracellular Ig-like domains which are important for ligand binding. They are highly expressed in vascular endothelial cells and are critical for the physiological and pathological growth, development and maintenance of blood and lymphatic vessels.Lymphokines: Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.Vascular Endothelial Growth Factor Receptor-1: A 180-kDa VEGF receptor found primarily in endothelial cells that is essential for vasculogenesis and vascular maintenance. It is also known as Flt-1 (fms-like tyrosine kinase receptor-1). A soluble, alternatively spliced isoform of the receptor may serve as a binding protein that regulates the availability of various ligands for VEGF receptor binding and signal transduction.Neovascularization, Pathologic: A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.Ristocetin: An antibiotic mixture of two components, A and B, obtained from Nocardia lurida (or the same substance produced by any other means). It is no longer used clinically because of its toxicity. It causes platelet agglutination and blood coagulation and is used to assay those functions in vitro.Factor VIII: Blood-coagulation factor VIII. Antihemophilic factor that is part of the factor VIII/von Willebrand factor complex. Factor VIII is produced in the liver and acts in the intrinsic pathway of blood coagulation. It serves as a cofactor in factor X activation and this action is markedly enhanced by small amounts of thrombin.Platelet Glycoprotein GPIb-IX Complex: Platelet membrane glycoprotein complex essential for normal platelet adhesion and clot formation at sites of vascular injury. It is composed of three polypeptides, GPIb alpha, GPIb beta, and GPIX. Glycoprotein Ib functions as a receptor for von Willebrand factor and for thrombin. Congenital deficiency of the GPIb-IX complex results in Bernard-Soulier syndrome. The platelet glycoprotein GPV associates with GPIb-IX and is also absent in Bernard-Soulier syndrome.Neovascularization, Physiologic: The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.Receptors, Growth Factor: Cell surface receptors that bind growth or trophic factors with high affinity, triggering intracellular responses which influence the growth, differentiation, or survival of cells.Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Vascular Endothelial Growth Factor C: A vascular endothelial growth factor that specifically binds to VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 and VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-3. In addition to being an angiogenic factor it can act on LYMPHATIC VESSELS to stimulate LYMPHANGIOGENESIS. It is similar in structure to VASCULAR ENDOTHELIAL GROWTH FACTOR D in that they both contain N- and C-terminal extensions that were not found in other VEGF family members.Platelet Adhesiveness: The process whereby PLATELETS adhere to something other than platelets, e.g., COLLAGEN; BASEMENT MEMBRANE; MICROFIBRILS; or other "foreign" surfaces.Vascular Endothelial Growth Factor Receptor-3: A vascular endothelial cell growth factor receptor whose expression is restricted primarily to adult lymphatic endothelium. VEGFR-3 preferentially binds the vascular endothelial growth factor C and vascular endothelial growth factor D and may be involved in the control of lymphangiogenesis.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Blood Coagulation Factors: Endogenous substances, usually proteins, that are involved in the blood coagulation process.Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Angiogenesis Inhibitors: Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.von Willebrand Disease, Type 2: A subtype of von Willebrand disease that results from qualitative deficiencies of VON WILLEBRAND FACTOR. The subtype is divided into several variants with each variant having a distinctive pattern of PLATELET-interaction.Weibel-Palade Bodies: Rod-shaped storage granules for VON WILLEBRAND FACTOR specific to endothelial cells.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Platelet Membrane Glycoproteins: Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.von Willebrand Disease, Type 1: A subtype of von Willebrand disease that results from a partial deficiency of VON WILLEBRAND FACTOR.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Vascular Endothelial Growth Factor D: A vascular endothelial growth factor that specifically binds to VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 and VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-3. In addition to being an angiogenic factor it can act on LYMPHATIC VESSELS to stimulate LYMPHANGIOGENESIS. It is similar in structure to VASCULAR ENDOTHELIAL GROWTH FACTOR C in that they both contain N- and C-terminal extensions that were not found in other VEGF family members.Vascular Endothelial Growth Factor B: A vascular endothelial growth factor expressed in a variety of tissues. It binds with high specificity to VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-1 and NEUROPILIN-1.Fibroblast Growth Factor 2: A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).Receptor Protein-Tyrosine Kinases: A class of cellular receptors that have an intrinsic PROTEIN-TYROSINE KINASE activity.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.ADAM Proteins: A family of membrane-anchored glycoproteins that contain a disintegrin and metalloprotease domain. They are responsible for the proteolytic cleavage of many transmembrane proteins and the release of their extracellular domain.Platelet Aggregation: The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.Deamino Arginine Vasopressin: A synthetic analog of the pituitary hormone, ARGININE VASOPRESSIN. Its action is mediated by the VASOPRESSIN receptor V2. It has prolonged antidiuretic activity, but little pressor effects. It also modulates levels of circulating FACTOR VIII and VON WILLEBRAND FACTOR.Umbilical Veins: Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.Hypoxia-Inducible Factor 1, alpha Subunit: Hypoxia-inducible factor 1, alpha subunit is a basic helix-loop-helix transcription factor that is regulated by OXYGEN availability and is targeted for degradation by VHL TUMOR SUPPRESSOR PROTEIN.Bleeding Time: Duration of blood flow after skin puncture. This test is used as a measure of capillary and platelet function.Neuropilin-1: Dimeric cell surface receptor involved in angiogenesis (NEOVASCULARIZATION, PHYSIOLOGICAL) and axonal guidance. Neuropilin-1 is a 140-kDa transmembrane protein that binds CLASS 3 SEMAPHORINS, and several other growth factors. Neuropilin-1 forms complexes with plexins or VEGF RECEPTORS, and binding affinity and specificity are determined by the composition of the neuropilin dimer and the identity of other receptors complexed with it. Neuropilin-1 is expressed in distinct patterns during neural development, complementary to those described for NEUROPILIN-2.Intercellular Signaling Peptides and Proteins: Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.Crotalid Venoms: Venoms from snakes of the subfamily Crotalinae or pit vipers, found mostly in the Americas. They include the rattlesnake, cottonmouth, fer-de-lance, bushmaster, and American copperhead. Their venoms contain nontoxic proteins, cardio-, hemo-, cyto-, and neurotoxins, and many enzymes, especially phospholipases A. Many of the toxins have been characterized.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Angiogenesis Inducing Agents: Agents that induce or stimulate PHYSIOLOGIC ANGIOGENESIS or PATHOLOGIC ANGIOGENESIS.Angiopoietin-2: An angiopoietin that is closely related to ANGIOPOIETIN-1. It binds to the TIE-2 RECEPTOR without receptor stimulation and antagonizes the effect of ANGIOPOIETIN-1. However its antagonistic effect may be limited to cell receptors that occur within the vasculature. Angiopoietin-2 may therefore play a role in down-regulation of BLOOD VESSEL branching and sprouting.Angiopoietin-1: The first to be discovered member of the angiopoietin family. It may play a role in increasing the sprouting and branching of BLOOD VESSELS. Angiopoietin-1 specifically binds to and stimulates the TIE-2 RECEPTOR. Several isoforms of angiopoietin-1 occur due to ALTERNATIVE SPLICING of its mRNA.Hemostasis: The process which spontaneously arrests the flow of BLOOD from vessels carrying blood under pressure. It is accomplished by contraction of the vessels, adhesion and aggregation of formed blood elements (eg. ERYTHROCYTE AGGREGATION), and the process of BLOOD COAGULATION.Antigens, CD31: Cell adhesion molecules present on virtually all monocytes, platelets, and granulocytes. CD31 is highly expressed on endothelial cells and concentrated at the junctions between them.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Capillaries: The minute vessels that connect the arterioles and venules.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Fibrinogen: Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Epidermal Growth Factor: A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Purpura, Thrombotic Thrombocytopenic: An acquired, congenital, or familial disorder caused by PLATELET AGGREGATION with THROMBOSIS in terminal arterioles and capillaries. Clinical features include THROMBOCYTOPENIA; HEMOLYTIC ANEMIA; AZOTEMIA; FEVER; and thrombotic microangiopathy. The classical form also includes neurological symptoms and end-organ damage, such as RENAL FAILURE.Cell Hypoxia: A condition of decreased oxygen content at the cellular level.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Cell Line, Tumor: A cell line derived from cultured tumor cells.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Receptor, Epidermal Growth Factor: A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.Pregnancy Proteins: Proteins produced by organs of the mother or the PLACENTA during PREGNANCY. These proteins may be pregnancy-specific (present only during pregnancy) or pregnancy-associated (present during pregnancy or under other conditions such as hormone therapy or certain malignancies.)Blood Vessels: Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Human Umbilical Vein Endothelial Cells: Endothelial cells that line venous vessels of the UMBILICAL CORD.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Platelet Activation: A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Hypoxia-Inducible Factor 1: A basic helix-loop-helix transcription factor that plays a role in APOPTOSIS. It is composed of two subunits: ARYL HYDROCARBON RECEPTOR NUCLEAR TRANSLOCATOR and HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Capillary Permeability: The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Mice, Inbred C57BLBiological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Microcirculation: The circulation of the BLOOD through the MICROVASCULAR NETWORK.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Transforming Growth Factor beta: A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.von Willebrand Disease, Type 3: A subtype of von Willebrand disease that results from a total or near total deficiency of VON WILLEBRAND FACTOR.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Receptor, TIE-2: A TIE receptor tyrosine kinase that is found almost exclusively on ENDOTHELIAL CELLS. It is required for both normal embryonic vascular development (NEOVASCULARIZATION, PHYSIOLOGIC) and tumor angiogenesis (NEOVASCULARIZATION, PATHOLOGIC).Bernard-Soulier Syndrome: A familial coagulation disorder characterized by a prolonged bleeding time, unusually large platelets, and impaired prothrombin consumption.Lymphangiogenesis: The formation of LYMPHATIC VESSELS.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Endothelium: A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.Hepatocyte Growth Factor: Multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. Its receptor is PROTO-ONCOGENE PROTEINS C-MET.Thrombin: An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Thrombosis: Formation and development of a thrombus or blood clot in the blood vessel.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Platelet-Derived Growth Factor: Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Retinal Neovascularization: Formation of new blood vessels originating from the retinal veins and extending along the inner (vitreal) surface of the retina.Pyrroles: Azoles of one NITROGEN and two double bonds that have aromatic chemical properties.Ischemia: A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.Angiogenic Proteins: Intercellular signaling peptides and proteins that regulate the proliferation of new blood vessels under normal physiological conditions (ANGIOGENESIS, PHYSIOLOGICAL). Aberrant expression of angiogenic proteins during disease states such as tumorigenesis can also result in PATHOLOGICAL ANGIOGENESIS.Corneal Neovascularization: New blood vessels originating from the corneal veins and extending from the limbus into the adjacent CORNEAL STROMA. Neovascularization in the superficial and/or deep corneal stroma is a sequel to numerous inflammatory diseases of the ocular anterior segment, such as TRACHOMA, viral interstitial KERATITIS, microbial KERATOCONJUNCTIVITIS, and the immune response elicited by CORNEAL TRANSPLANTATION.Growth Substances: Signal molecules that are involved in the control of cell growth and differentiation.Retinal Vessels: The blood vessels which supply and drain the RETINA.Stress, Mechanical: A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.Antibodies, Monoclonal, Humanized: Antibodies from non-human species whose protein sequences have been modified to make them nearly identical with human antibodies. If the constant region and part of the variable region are replaced, they are called humanized. If only the constant region is modified they are called chimeric. INN names for humanized antibodies end in -zumab.Cell Adhesion: Adherence of cells to surfaces or to other cells.Insulin-Like Growth Factor I: A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor.P-Selectin: Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.Fibroblast Growth Factors: A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Lymphatic Vessels: Tubular vessels that are involved in the transport of LYMPH and LYMPHOCYTES.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Anoxia: Relatively complete absence of oxygen in one or more tissues.Thrombospondin 1: An extracellular matrix glycoprotein from platelets and a variety of normal and transformed cells of both mesenchymal and epithelial origin. Thrombospondin-1 is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. Also, it has been studied for its use as a potential regulator of tumor growth and metastasis.Metalloendopeptidases: ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism.Hemophilia A: The classic hemophilia resulting from a deficiency of factor VIII. It is an inherited disorder of blood coagulation characterized by a permanent tendency to hemorrhage.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Blood Coagulation Tests: Laboratory tests for evaluating the individual's clotting mechanism.PhthalazinesProtein Isoforms: Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.Receptors, Cell Surface: Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Blood Coagulation: The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.Indoles: Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Transplantation, Heterologous: Transplantation between animals of different species.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Culture Media, Conditioned: Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).Xenograft Model Antitumor Assays: In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.Transforming Growth Factor alpha: An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.Thrombospondins: A family of related, adhesive glycoproteins which are synthesized, secreted, and incorporated into the extracellular matrix of a variety of cells, including alpha granules of platelets following thrombin activation and endothelial cells. They interact with a number of BLOOD COAGULATION FACTORS and anticoagulant factors. Five distinct forms have been identified, thrombospondin 1, -2, -3, -4, and cartilage oligomeric matrix protein (COMP). They are involved in cell adhesion, platelet aggregation, cell proliferation, angiogenesis, tumor metastasis, VASCULAR SMOOTH MUSCLE growth, and tissue repair.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Microvessels: The finer blood vessels of the vasculature that are generally less than 100 microns in internal diameter.Neoplasm Transplantation: Experimental transplantation of neoplasms in laboratory animals for research purposes.Neuropilin-2: Transmembrane receptor for CLASS 3 SEMAPHORINS and several vascular endothelial growth factor isoforms. Neuropilin-2 functions either as a homodimer or as a heterodimer with NEUROPILIN-1. The binding affinity of neuropilin-2 varies for different class 3 semaphorin isoforms and is dependent on the composition of the dimer. The protein also forms receptor complexes with plexins and with VEGF RECEPTORS, which alters the binding characteristics of the receptor.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.Nerve Growth Factors: Factors which enhance the growth potentialities of sensory and sympathetic nerve cells.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Transforming Growth Factor beta1: A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.Wound Healing: Restoration of integrity to traumatized tissue.Von Hippel-Lindau Tumor Suppressor Protein: A ubiquitin-protein ligase that mediates OXYGEN-dependent polyubiquitination of HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT. It is inactivated in VON HIPPEL-LINDAU SYNDROME.Thrombomodulin: A cell surface glycoprotein of endothelial cells that binds thrombin and serves as a cofactor in the activation of protein C and its regulation of blood coagulation.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Mice, Inbred BALB CAntineoplastic Agents: Substances that inhibit or prevent the proliferation of NEOPLASMS.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Heparin: A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.Blood Platelet Disorders: Disorders caused by abnormalities in platelet count or function.Chorioallantoic Membrane: A highly vascularized extra-embryonic membrane, formed by the fusion of the CHORION and the ALLANTOIS. It is mostly found in BIRDS and REPTILES. It serves as a model for studying tumor or cell biology, such as angiogenesis and TISSUE TRANSPLANTATION.Macromolecular Substances: Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.Hindlimb: Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Endostatins: Angiostatic proteins that are formed from proteolytic cleavage of COLLAGEN TYPE XVIII.Proto-Oncogene Proteins c-akt: A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.Kinetics: The rate dynamics in chemical or physical systems.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Tumor Markers, Biological: Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.von Hippel-Lindau Disease: An autosomal dominant disorder caused by mutations in a tumor suppressor gene. This syndrome is characterized by abnormal growth of small blood vessels leading to a host of neoplasms. They include HEMANGIOBLASTOMA in the RETINA; CEREBELLUM; and SPINAL CORD; PHEOCHROMOCYTOMA; pancreatic tumors; and renal cell carcinoma (see CARCINOMA, RENAL CELL). Common clinical signs include HYPERTENSION and neurological dysfunctions.Phosphatidylinositol 3-Kinases: Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Transforming Growth Factors: Hormonally active polypeptides that can induce the transformed phenotype when added to normal, non-transformed cells. They have been found in culture fluids from retrovirally transformed cells and in tumor-derived cells as well as in non-neoplastic sources. Their transforming activities are due to the simultaneous action of two otherwise unrelated factors, TRANSFORMING GROWTH FACTOR ALPHA and TRANSFORMING GROWTH FACTOR BETA.Protein Kinase Inhibitors: Agents that inhibit PROTEIN KINASES.Hemorheology: The deformation and flow behavior of BLOOD and its elements i.e., PLASMA; ERYTHROCYTES; WHITE BLOOD CELLS; and BLOOD PLATELETS.Genetic Therapy: Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.Diabetic Retinopathy: Disease of the RETINA as a complication of DIABETES MELLITUS. It is characterized by the progressive microvascular complications, such as ANEURYSM, interretinal EDEMA, and intraocular PATHOLOGIC NEOVASCULARIZATION.Receptors, Mitogen: Glycoprotein molecules on the surface of B- and T-lymphocytes, that react with molecules of antilymphocyte sera, lectins, and other agents which induce blast transformation of lymphocytes.Pericytes: Unique slender cells with multiple processes extending along the capillary vessel axis and encircling the vascular wall, also called mural cells. Pericytes are imbedded in the BASEMENT MEMBRANE shared with the ENDOTHELIAL CELLS of the vessel. Pericytes are important in maintaining vessel integrity, angiogenesis, and vascular remodeling.Aurintricarboxylic Acid: A dye which inhibits protein biosynthesis at the initial stages. The ammonium salt (aluminon) is a reagent for the colorimetric estimation of aluminum in water, foods, and tissues.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Molecular Weight: The sum of the weight of all the atoms in a molecule.Extracellular Matrix Proteins: Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Lung Neoplasms: Tumors or cancer of the LUNG.QuinazolinesKidney Neoplasms: Tumors or cancers of the KIDNEY.Platelet Count: The number of PLATELETS per unit volume in a sample of venous BLOOD.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Carcinoma, Renal Cell: A heterogeneous group of sporadic or hereditary carcinoma derived from cells of the KIDNEYS. There are several subtypes including the clear cells, the papillary, the chromophobe, the collecting duct, the spindle cells (sarcomatoid), or mixed cell-type carcinoma.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Aorta: The main trunk of the systemic arteries.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Afibrinogenemia: A deficiency or absence of FIBRINOGEN in the blood.Vascular Endothelial Growth Factor, Endocrine-Gland-Derived: A vascular endothelial growth factor whose expression is found largely restricted to the GONADS; ADRENAL CORTEX; and PLACENTA. It has similar biological activity to VASCULAR ENDOTHELIAL GROWTH FACTOR-A.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Primary hypertrophic osteoathropathy
... such as Von Willebrand factor and vascular endothelial growth factor (VEGF). These substances could also have a role in PDP ... Von Willebrand factor is a marker of platelet and endothelial activation. This suggests that the activation of endothelial ... 2000). "Vascular endothelial growth factor and hypertrophic osteoarthropathy". Clin Exp Rheumatol. 18: 57-62. Jin Hyun Kim; et ... platelet-derived growth factor (PDGF) and epidermal growth factor (EGF). It has not been described yet what role these ...
List of proteins
Fibroblast growth factor (FGF) Platelet-derived growth factor (PDGF) Transforming growth factors (TGFs) Vascular endothelial ... Factor VIII Factor XIII Protein C Protein S Protein Z Protein Z-related protease inhibitor Thrombin Von Willebrand Factor C- ... growth factor (VEGF) Peptide hormones Insulin Insulin-like growth factor (IGF) Oxytocin Steroid hormones Sex steroids are not ... ion channels Potassium channels Calcium channels Sodium channels Glucose transporter Growth factors Colony-stimulating factors ...
A lack of pericytes also causes an upregulation of vascular endothelial growth factor (VEGF), leading to vascular leakage and ... The vascular markers CD 31, von Willebrand factor (vWF), and smooth muscle actin (pericyte marker) are present during the ... vascular endothelial growth factor (VEGF), and type IV collagenase, the former two localized to both endothelium and pericytes ... "Tumor-derived vascular endothelial growth factor up-regulates angiopoietin-2 in host endothelium and destabilizes host ...
Proteases in angiogenesis
Several growth factors such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) are trapped ... and ADAMTS13 which cleaves von Willebrand factor. Unlike with MMPs, TIMPs are more selective in their ability to inhibit ADAMs ... Dvorak, H; Brown, LF; Detmar, M; Dvorak, AM (1995). "Vascular permeability factor/vascular endothelial growth factor, ... Hepatocyte growth factor (HGF), an angiogenesis promoting growth factor, is activated by HGF activation factor, a serine ...
Leukocyte adhesion to the damaged endothelial wall and abnormal von Willebrand factor (or vWF) release can also contribute to ... The repression of the vascular endothelial growth factor (VEGF) can also cause glomerular TMA (damage to the glomerular ... The cytotoxicity from the lack of protein damages glomerular endothelial cells by creating voids in the endothelial wall and ... Thrombotic microangiopathy (TMA) is a pathology that results in thrombosis in capillaries and arterioles, due to an endothelial ...
... an upstream effector of vascular endothelial growth factor (VEGF), MCL1, and cyclooxygenase 2 (COX-2), all significant pro- ... cause hemorrhage especially in patients with conditions that would put them at a higher risk like hemophilia or Von Willebrand ... This mechanism is believed to suppress production of NO, tumor necrosis factor-α (TNF-α), and RANTES/CCL5. Honokiol has also ... Hu, He; Zhang, Xiao-xue; Wang, Yin-ye; Chen, Shi-Zhong (2005). "Honokiol inhibits arterial thrombosis through endothelial cell ...
Hattori R, Hamilton KK, Fugate RD, McEver RP, Sims PJ (May 1989). "Stimulated secretion of endothelial von Willebrand factor is ... is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies". J. Clin. Invest. 84 (1): 92-9. doi ... platelet and helps cancer cells invade into bloodstream for metastasis and provided locally with multiple growth factors ... "Differential regulation of endothelial exocytosis of P-selectin and von Willebrand factor by protease-activated receptors and ...
The von Willebrand factor (VWF) serves as an essential accessory molecule. In general terms, platelet activation initiated by ... FGF (Fibroblast Growth Factor) ligands bind to receptors tyrosine kinase, FGFR (Fibroblast Growth Factor Receptors), and form a ... After a vascular injury occurs, platelets are activated by locally exposed collagen (glycoprotein (GP) VI receptor), locally ... adhesion of leukocytes to endothelial cells is related with morphological changes in both leukocytes and endothelial cells, ...
... fibroblast growth factor, insulin-like growth factor 1, platelet-derived epidermal growth factor, and vascular endothelial ... Endothelial cells are attached to the subendothelial collagen by von Willebrand factor (VWF) which these cells produce. VWF is ... transforming growth factor-β1, platelet-derived growth factor, fibronectin, B-thromboglobulin, vWF, fibrinogen, and coagulation ... The excessive numbers create a relative von Willebrand factor deficiency due to sequestration.) One can get a clue as to ...
A further consequence of damage to the endothelium is the release of pathological quantities of von Willebrand factor, which ... Endothelial cells in direct contact with blood are called vascular endothelial cells, whereas those in direct contact with ... Platelet activation Susac's syndrome Tunica intima VE-cadherin Weibel-Palade body Angiocrine growth factors "Endothelium" at ... dewetting Endothelial activation Endothelial microparticle Endothelial progenitor cell Endothelium-derived relaxing factor ( ...
... spontaneous platelet accumulation may be caused by contact with pig von Willebrand factor. ... Acute vascular rejection requires de novo protein synthesis and is driven by interactions between the graft endothelial cells ... activate macrophages to secrete smooth muscle growth factors. This results in a build up of smooth muscle cells on the vessel ... Overcoming acute vascular rejection. Due to its complexity, the use of immunosuppressive drugs along with a wide array of ...
The four CYR61 domains are, from N- to C-termini, the insulin-like growth factor binding protein (IGFBP) domain, von Willebrand ... "Vascular endothelial growth factor (VEGF)-induced up-regulation of CCN1 in osteoblasts mediates proangiogenic activities in ... including stimulation by platelet-derived growth factor and basic fibroblast growth factor, transforming growth factor β1 (TGF- ... product of a growth factor-inducible immediate-early gene, promotes angiogenesis and tumor growth". Proc. Natl. Acad. Sci. U.S. ...
... von Willebrand type C repeats (vWC), thrombospondin type 1 repeat (TSR), and a cysteine knot motif within the C-terminal (CT) ... connective tissue growth factor, or CCN2), and NOV. These proteins, together with WISP1 (CCN4), WISP2 (CCN5), and WISP3 (CCN6) ... Ellis PD, Metcalfe JC, Hyvönen M, Kemp PR (2003). "Adhesion of endothelial cells to NOV is mediated by the integrins ... However, Nov-null mice show enhanced blood vessel neointimal thickening when challenged with vascular injury, indicating that ...
Additionally, spontaneous platelet accumulation may be caused by contact with pig von Willebrand factor. Just as the α1,3G ... Acute vascular rejection requires de novo protein synthesis and is driven by interactions between the graft endothelial cells ... activate macrophages to secrete smooth muscle growth factors. This results in a build up of smooth muscle cells on the vessel ... von Derdidas, Ihrwir (2009) A More Modest Proposal Archived July 12, 2011, at the Wayback Machine.. Hotel St. George Press. ...
Von Willebrand factor
... transforming growth factor-β (TGFβ) and β-human chorionic gonadotropin (βHCG, of pregnancy test fame). (Von Willebrand factor ... which would explain why some people with von Willebrand disease develop vascular malformations (predominantly in the digestive ... factor VIII has a half-life of 8-12 hours. vWF binds to collagen, e.g., when it is exposed in endothelial cells due to damage ... Includes: Type 1 von Willebrand Disease, Type 2A von Willebrand Disease, Type 2B von Willebrand Disease, Type 2M von Willebrand ...
A further consequence of damage to the endothelium is the release of pathological quantities of von Willebrand factor, which ... Endothelial dysfunction, or the loss of proper endothelial function, is a hallmark for vascular diseases, and is often regarded ... Endothelial cells in direct contact with blood are called vascular endothelial cells, whereas those in direct contact with ... Vascular endothelial cells line the entire circulatory system, from the heart to the smallest capillaries. These cells have ...
Inhibition of Robo1, which colocalizes with von Willebrand factor in tumor endothelial cells, leads to reduced micro-vessel ... "Robo4 stabilizes the vascular network by inhibiting pathologic angiogenesis and endothelial hyperpermeability". Nat. Med. 14 (4 ... Seeger M, Tear G, Ferres-Marco D, Goodman CS (March 1993). "Mutations affecting growth cone guidance in Drosophila: genes ... Slit-Robo has been shown to influence the migration of neurons and glia, leukocytes, and endothelial cells. Slit1 and Slit2 ...
Endothelial progenitor cell
von Willebrand factor expression +/- +/- + ALDH Bright Bright Bright acLDL uptake + + + Colony forming unit - Hill. As ... marrow-derived circulating endothelial precursors do not contribute to vascular endothelium and are not needed for tumor growth ... Role in tumor growth. Endothelial progenitor cells are likely important in tumour growth and are thought to be critical ... Ablation of the endothelial progenitor cells in the bone marrow lead to a significant decrease in tumour growth and vasculature ...
Deep vein thrombosis
Individuals without O blood type have higher blood levels of von Willebrand factor and factor VIII than those with O blood type ... Hypoxia-inducible factor-1 and early-growth-response protein 1 contribute to monocyte association with endothelial proteins, ... both of which may increase the risk because of tissue factor from outside the vascular system entering the blood. In orthopedic ... The three factors of Virchow's triad-venous stasis, hypercoagulability, and changes in the endothelial blood vessel lining ( ...
... fibroblast growth factor, insulin-like growth factor 1, platelet-derived epidermal growth factor, and vascular endothelial ... Endothelial cells are attached to the subendothelial collagen by von Willebrand factor (VWF) which these cells produce. VWF is ... α granules (alpha granules) - containing P-selectin, platelet factor 4, transforming growth factor-β1, platelet-derived growth ... The excessive numbers create a relative von Willebrand factor deficiency due to sequestration.) ...
AP-1 transcription factor
Shiga toxins promote endothelial-cell secretion and impair ADAMTS13 cleavage of unusually large von Willebrand factor multimers ... Chang CJ, Chao JC (April 2002). "Effect of human milk and epidermal growth factor on growth of human intestinal Caco-2 cells". ... gene expression in cultured human vascular smooth muscle cells". Diabetologia. 44 (6): 713-20. doi:10.1007/s001250051680. PMID ... "The AP-1 transcription factor regulates breast cancer cell growth via cyclins and E2F factors". Oncogene. 27 (3): 366-377. doi: ...
... cleave connective tissue growth factor and reactivate angiogenic activity of vascular endothelial growth factor 165". J. Biol. ... These domains are, from N- to C-termini, the insulin-like growth factor binding protein (IGFBP) domain, the von Willebrand type ... "Role and interaction of connective tissue growth factor with transforming growth factor-beta in persistent fibrosis: A mouse ... Babic AM, Chen CC, Lau LF (April 1999). "Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion and ...
Duffy antigen system
... von Willebrand factor, CD31, CD34, CD105 and CD146". J. Pathol. 206 (3): 260-8. doi:10.1002/path.1788. PMID 15887283. Segerer S ... Rot A (2005). "Contribution of Duffy antigen to chemokine function". Cytokine Growth Factor Rev. 16 (6): 687-94. doi:10.1016/j. ... The Duffy antigen is present in the normal pulmonary vascular bed. Its expression is increased in the vascular beds and ... Hadley TJ, Lu ZH, Wasniowska K, Martin AW, Peiper SC, Hesselgesser J, Horuk R (1994). "Postcapillary venule endothelial cells ...
... including increased factor IX, von Willebrand factor, thrombin-antithrombin complex (TAT), fragment 1+2, and D-dimer and ... This is because they suppress insulin-like growth factor 1 production in the liver. Estrogens can be used to suppress lactation ... "Oral vs Transdermal Estrogen Therapy and Vascular Events: A Systematic Review and Meta-Analysis". J. Clin. Endocrinol. Metab. ... it raises HDL levels and has endothelial vasodilatation properties plus an anti-inflammatory component. Research is underway to ...
... or CD31 and von Willebrand factor (endothelial progenitor markers). This indicates that Muse cells do not belong to previously ... In the presence of insulin-transferrin-selenium, dexamethasone, hepatocyte growth factor, and fibroblast growth factor-4, Muse ... vascular endothelial cells, and other cell types in the dermis. Ulcers treated with human Muse cells heal faster with a thick ... The cells positive for MAP-2 or GFAP were increased following induction with basic fibroblast growth factor, forskolin, and ...
Disseminated intravascular coagulation
von Willebrand disease. *Hypoprothrombinemia/II. *Factor VII deficiency. *Factor X deficiency. *Factor XII deficiency ... including endothelial cells, macrophages, and monocytes) and is not normally in contact with the general circulation, but is ... and increased vascular permeability. The acute form of DIC is considered an extreme expression of the intravascular coagulation ... Clotting factors are consumed in the development of multiple clots, which contributes to the bleeding seen with DIC.[citation ...
"Vasopressin-induced von Willebrand factor secretion from endothelial cells involves V2 receptors and cAMP". The Journal of ... Second, AVP constricts arterioles, which increases peripheral vascular resistance and raises arterial blood pressure. ... positive regulation of cell growth. • positive regulation of cAMP biosynthetic process. • positive regulation of prostaglandin ... Vasoconstriction, gluconeogenesis, platelet aggregation, and release of factor VIII and von Willebrand factor; social ...
Gastric cancer-associated enhancement of von Willebrand factor is regulated by vascular endothelial growth factor and related...
The role of vascular endothelial growth factor (VEGF) in differential vWF expression was investigated using cultured human ... tumor growth, and metastasis. Yet, the expression pattern of vWF in human gastric cancer (GC) tissues and its relation to ... umbilical vein endothelial cells (HUVECs). vWF and VEGF protein and mRNA expression levels were investigated by qRT-PCR, ... von Willebrand factor (vWF) is a potent regulator of angiogenesis, ...
Interleukin-10 overexpression improves the function of endothelial progenitor cells stimulated with TNF-α through the...
... von Willebrand factor; PE-VEGFR2, phycoerythrin-vascular endothelial growth factor receptor 2; GFP, green fluorescent protein; ... EPCs were cultured and identified by fluorescent labeling with the von Willebrand factor antibody, vascular endothelial growth ... vascular endothelial growth factor; EGM-2, endothelial cell growth medium-2. ... EPCs, endothelial progenitor cells; VEGF, vascular endothelial growth factor; MMP-9, matrix metalloproteinase 9; p-STAT3, ...
Primary hypertrophic osteoathropathy - Wikipedia
... such as Von Willebrand factor and vascular endothelial growth factor (VEGF). These substances could also have a role in PDP ... Von Willebrand factor is a marker of platelet and endothelial activation. This suggests that the activation of endothelial ... 2000). "Vascular endothelial growth factor and hypertrophic osteoarthropathy". Clin Exp Rheumatol. 18: 57-62. Jin Hyun Kim; et ... platelet-derived growth factor (PDGF) and epidermal growth factor (EGF). It has not been described yet what role these ...
von Willebrand Factor Summary Report | CureHunter
... produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von ... The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor. ... Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct ... von Willebrand Factor: A high-molecular-weight plasma protein, ... Vascular Endothelial Growth Factor A (Vascular Endothelial ...
Atrial Fibrillation Pathophysiology | Circulation
... tumor necrosis factor-α; VEGF, vascular endothelial growth factor; TGF-β1, transforming growth factor-β1; F1+2, prothrombin ... vWF indicates von Willebrand factor; NOS, nitric oxide synthase; TF, tissue factor; TFPI, tissue factor pathway inhibitor; TM, ... Autonomic nervous system factors are important in AF.24 Vagal discharge enhances acetylcholine-dependent K+ current (IKACh), ... Thrombomodulin and tissue factor pathway inhibitor in endocardium of rapidly paced rat atria. Circulation. 2003;108:2450-2452. ...
Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive factor
We show that NO inhibits exocytosis of Weibel-Palade bodies, endothelial granules that mediate vascular inflammation and ... inhibits vascular inflammation, but the molecular basis for its anti-inflammatory properties is unknown. ... Vascular Endothelial Growth Factor A * Vesicular Transport Proteins * von Willebrand Factor * Nitric Oxide ... We show that NO inhibits exocytosis of Weibel-Palade bodies, endothelial granules that mediate vascular inflammation and ...
Biomarkers and acute brain injuries: interest and limits | Critical Care | Full Text
... vascular cell adhesion molecule-1; VEGF, vascular endothelial growth factor; vWF, von Willebrand factor; WFNS, World Federation ... vascular cell adhesion molecule-1; VEGF, vascular endothelial growth factor; vWF, von Willebrand factor. ... von Willebrand factor, vascular endothelial growth factor and matrix metalloproteinase-9. During cerebral vasospasm, sustained ... Serum von Willebrand factor, matrix metalloproteinase-9, and vascular endothelial growth factor levels predict the onset of ...
Frontiers | Novel Insights in Systemic Lupus Erythematosus and Atherosclerosis | Medicine
... but the combination of several biomarkers and risk factors may better estimate cardiovascular disease risk. Moreover, the ... but the combination of several biomarkers and risk factors may better estimate cardiovascular disease risk. Moreover, the ... vascular cell adhesion molecule-1; VEGF, vascular endothelial growth factor; vWf, von Willebrand factor. ... role of vascular endothelial growth factor (VEGATS Study). Autoimmun Rev (2009) 8(4):309-15. doi:10.1016/j.autrev.2008.10.002 ...
Rabbit von Willebrand Factor,VWF ELISA kit EK2354 兔血管内皮细胞生长因子(VEGF)ELISA试剂盒 Rabbit Vascular Endothelial cell Growth Factor,VEGF ... rabbit Insulin-like growth factor 2,IGF-2 ELISA kit EK2388 兔子胰岛素样生长因子1(IGF-1)ELISA试剂盒 rabbit Insulin-like growth factor 1,IGF-1 ... guinea pig transforming growth factor β1,TGF-β1 ELISA kit EK2481 豚鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒 Guinea pig Tumor necrosis factor α, ... rabbit Transforming Growth factor β1,TGF-β1 ELISA kit
Trauma-Induced Coagulopathy | SpringerLink
Ang-2 is a vascular endothelial growth factor (VEGF) that modulates inflammation and increases vascular permeability . Ang- ... Weibel-Palade bodies contain tissue plasminogen activator (TPA), Von Willebrand factor antigen, thrombomodulin, and angiopoetin ... First, APC cleaves procoagulant Factors V and VIII. Both of these factors are essential cofactors in the production of thrombin ... Endothelial damage results in degradation of the endothelial glycocalyx (EG), a 1-μm thick, anti-adhesive and anticoagulant ...
Clinical Hemorheology and Microcirculation - Volume 67, issue 2 - Journals - IOS Press
Von Willebrand factor, vascular endothelial growth factor, and angiopoietin-2 expression were not significantly different ... Abstract: BACKGROUND: Interaction of von Willebrand factor (VWF) with circulating platelets is the trigger for thrombosis in a ... We incubated ASCs in α -MEM with different Platelet derived growth factor (PDGF) subtypes or 10% or 20% pooled PRP or 20% fetal ... Impact Factor 2019: 1.642 Clinical Hemorheology and Microcirculation, a peer-reviewed international scientific journal, serves ...
Adipose-derived Mesenchymal Stem Cells and Their Reparative Potential in Ischemic Heart Disease | Revista Española de...
... vascular endothelial; VEGFR2, vascular endothelial growth factor 2; vWF, von Willebrand factor. ... vascular endothelial; VEGFR2, vascular endothelial growth factor 2; vWF, von Willebrand factor. ... stromal vascular fraction; TGFβ-1, transforming growth factor β1; VEGF, vascular endothelial growth factor. ... Hepatocyte growth factor or vascular endothelial growth factor gene transfer maximizes mesenchymal stem cell-based myocardial ...
JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... the expression of transforming growth factor??1 (TGF??1), vascular endothelial growth factor (VEGF) and von Willebrand factor ( ... With the growth of disease incidence in allergic diseases of upper respiratory tract year by year, airborne pollen has been ... Transcription factor Sp1 is multifaceted, with the ability to function as an oncogene or a tumor suppressor, depending on the ... In addition, other factors, such as the aromaticity and hydrophobicity, also influence the codon usage variation among HN and F ...
Search Results | jns
... cells in crushed nerves paralleled the elevated expressions of von Willebrand factor, isolectin B4, and vascular endothelial ... Significant differences in expression of nerve growth factor existed in skin, and the differences were associated with the ... Stromal cell-derived factor-1α increased the migration and wound healing of AFMSCs in vitro, and the migration ability was dose ... The expression of stromal cell-derived factor-1α (SDF-1α) in the injured nerve exerts a trophic effect by recruiting progenitor ...
Effect of aerobic and resistance training on inflammatory markers in heart failure patients: systematic review and meta...
... von Willebrand factor, tissue factor and vascular endothelial growth factor levels in congestive heart failure. Eur J Clin ... Kato M, Masuda T, Ogano M et al (2017) Stretching exercises improve vascular endothelial dysfunction through attenuation of ... Chronic heart failure and aging-effects of exercise training on endothelial function and mechanisms of endothelial regeneration ... Levine B, Kalman J, Mayer L, Fillit HM, Packer M (1990) Elevated circulating levels of tumor necrosis factor in severe chronic ...
Coronary vein infusion of multipotent stromal cells from bone marrow preserves cardiac function in swine ischemic...
... basic fibroblast growth factor and vascular endothelial growth factor (VEGF) levels were significantly higher in the MSC- ... A few MSCs expressed von Willebrand factor in a differentiation assay, but none of them expressed troponin T. In quantitative ... Vascular Endothelial Growth Factor A/metabolism. Substances. *Vascular Endothelial Growth Factor A ... In vitro experiment demonstrated that MSCs significantly stimulated endothelial capillary network formation compared with the ...
Simvastatin enhances bone marrow stromal ... & related info | Mendeley
Simvastatin enhances bone marrow stromal cell differentiation into endothelial cells via notch signaling pathway. Am J Phy... ... including von Willebrand factor (vWF), CD31, vascular endothelial-cadherin (VE-cadherin), vascular endothelial growth factor ... blocked the expression of endothelial-specific markers in BMSCs and their differentiation into functional endothelial cells. ... Simvastatin enhances bone marrow stromal cell differentiation into endothelial cells via notch signaling pathway. *Xu J ...
Visualizing Vascular Endothelial Growth Factor (VEGF) Producing Lesions in Von Hippel-Lindau Disease - Tabular View -...
... placental growth factor (PlGF), soluble VEGF receptors) and endothelial activation markers (such as Von Willebrand Factor (VWF ... Visualizing Vascular Endothelial Growth Factor (VEGF) Producing Lesions in Von Hippel-Lindau Disease (VHLimage). The safety and ... Visualizing Vascular Endothelial Growth Factor (VEGF) Producing Lesions in Von Hippel-Lindau Disease ... in the different disease associated lesions which results in production of high amounts of vascular endothelial growth factor ( ...
Evaluation of Hypertension as a Predictor of Efficacy Bevacizumab in Metastatic Breast Cancer and Metastatic Colorectal Cancer ...
... the fraction soluble receptor factor vascular endothelial growth (sVEGFR1), interleukin 6 (IL-6) and von Willebrand factor ( ... such as placental growth factor (PIGF), the growth factor vascular endothelial dependent (VEGF), ... recognized marker of damage / endothelial dysfunction), among others], and its association with the efficacy to treatment with ...
Peptide blockade of HIFα degradation modulates cellular metabolism and angiogenesis | PNAS
... detected by immunohistochemistry for von Willebrand factor, ×100), staining for vascular endothelial growth factor (×400), and ... revealed by immunostaining for von Willebrand factor. Epidermal growth factor (EGF; 5 ng/ml), which is known to induce growth ... On day 5 the distribution of endothelial cells was determined by using Abs to von Willebrand factor (Dako). ... vascular endothelial growth factor (Santa Cruz Biotechnology), and smooth muscle cell actin (Dako) Abs by using Dako Envision ...
Advanced glycation end products impair the migration, adhesion and secretion potentials of late endothelial progenitor cells |...
Von Willebrand factor. VEGFR2: Vascular endothelial growth factor receptor 2. VE-cadherin: Vascular endothelial cadherin ... but also by secreting the soluble factors including a number of enzymes like matrix protein, growth factors and cytokines [18 ... growing evidence suggest that EPCs can keep endothelial integrity not only by differentiating into mature vascular endothelial ... Soluble factors released by endothelial progenitor cells promote migration of endothelial cells and cardiac resident progenitor ...
VEGF Is Involved in the Increase of Dermal Microvascular Permeability Induced by Tryptase
The exact mechanisms of mast cell tryptase promoting vascular permeability, however, are unclear and, therefore, we ... supernatant on the permeability of human dermal microvascular endothelial cells (HDMECs). Both tryptase and HMC-1 supernatant ... which are manifested with rapid edema and increases of vascular permeability. ... Primary antibody against von Willebrand factor (vWF), CD34, vascular endothelial growth factor (VEGF), fms-like tyrosine kinase ...
Weibel−Palade bodies at a glance | Journal of Cell Science
C-delta mediates von Willebrand factor secretion from endothelial cells in response to vascular endothelial growth factor (VEGF ... The roles of protein kinase C and intracellular Ca2+ in the secretion of von Willebrand factor from human vascular endothelial ... Reactive oxygen intermediates induce regulated secretion of von Willebrand factor from cultured human vascular endothelial ... 2005b). Vascular endothelial growth factor regulation of Weibel−Palade-body exocytosis. Blood 105, 207-214. doi:10.1182/blood- ...
Expression of the Antiangiogenic Factor 16K hPRL in Human HCT116 Colon Cancer Cells Inhibits Tumor Growth in Rag1−/− Mice |...
... bovine brain capillary endothelial; BCS, bovine calf serum; PAI-1, plasminogen activator inhibitor 1; vWF, von Willebrand ... 3 The abbreviations used are: bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; 16K hPRL, 16 ... protein kinases by vascular endothelial growth factor and basic fibroblast growth factor in capillary endothelial cells is ... Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo. Nature (Lond.), 362: ...
von Willebrand factor rescued by miR-24 inhibition facilitates the proliferation and migration of osteosarcoma cells in vitro |...
2015) Gastric cancer-associated enhancement of von Willebrand factor is regulated by vascular endothelial growth factor and ... 2000) Von Willebrands factor mediates the adherence of human tumoral cells to human endothelial cells and ticlopidine ... 2017) Functional assessment of von Willebrand factor expression by cancer cells of non-endothelial origin. Oncotarget 8, 13015- ... 2009) Factor VIII-von Willebrand factor complex inhibits osteoclastogenesis and controls cell survival. J. Biol. Chem. 284, ...
Human hepatic stem cells from fetal and postnatal donors | JEM
... endothelial cell markers (vascular endothelial growth factor receptor [VEGFr], von Willebrand factor, and platelet/endothelial ... for endothelial cell markers (CD34, VEGFr or KDR, von Willebrand factor, and CD31), and for mesenchymal markers, especially ... vascular endothelial growth factor receptor and desmin). If transferred to STO feeders, hHpSCs give rise to hepatoblasts, which ... endothelial (VEGFr, von Willebrands factor, and CD31), and mesenchymal (CD146, desmin, and α-smooth muscle actin) cell ...
Primary angiosarcoma of the small intestine with metastasis to the liver: a case report and review of the literature.
Von Willebrand factor, CD34, CD31, Ulex europaeus agglutinin 1, vascular endothelial growth factor, melanocytic markers (such ... Gentry RW,Dockerty MB,Glagett OT,Vascular malformations and vascular tumors of the gastrointestinal tractSurg Gynecol Obstet ... Positive for Factor VIII, collagen type IV and vimentin Not available Gastrointestinal bleeding Resection Died 1 year after the ... Positive for Factor VIII, collagen type IV Not available Not available Resection Died shortly after surgery ...
Isoform 111 of Vascular Endothelial Growth Factor (VEGF111) Improves Angiogenesis of Ovarian Tissue Xenotransplantation.
Effects Of Recombinant Human Erythropoietin On Platelet Activation In Acute Myocardial Infarction: Results Of A Double-Blind,...
Erythropoietin improves cardiac function through endothelial progenitor cell and vascular endothelial growth factor mediated ... rHuEpo did not alter bleeding time, platelet function assay closure time, von Willebrand factor levels, soluble P-selectin, or ... increased expression of erythropoietin and vascular endothelial growth factor receptors in myocytes and vascular endothelial ... The concentration of erythropoietin receptor, vascular endothelial growth factor receptor Flt-1 (R & D Systems, Inc. ...
Limited Systemic Sclerosis Patients with Pulmonary Arterial Hypertension Show Biomarkers of Inflammation and Vascular Injury
... including Von Willebrand Factor (vWF) , C-reactive protein (CRP) , and vascular endothelial growth factor (VEGF). VEGF ... and markers of vascular injury such as VCAM-1, VEGF, and von Willebrand Factor were found in lSSc-PAH subjects. ... Upregulation of fibroblast growth factor-2 by visfatin that promotes endothelial angiogenesis. Biochem Biophys Res Commun. 2009 ... Recombinant tumor necrosis factor increases pulmonary vascular permeability independent of neutrophils. Proc Natl Acad Sci U S ...
PlateletsCocultured endothelial cellsProgenitorAbstractInvolved in endothelial cellReceptorsPromoting vascular permeabilityApoptosisAngiogenic factorsThrombosisProliferationMicrovascular endothelialTissueMarkerAntigenCells and megakaryocytesHypoxiaVivoExpressionHematopoieticStromal cellsDifferentiateEpithelial and endothelialBlood vesselsInducesPostnatalFunctionalMalignantPlatelet-deriveProteinsHumanTumorsHepaticSerumRegeneration
- Platelets are a critical component for the first phase of hemostasis (formation of the platelet plug), which can halt the loss of blood from vessels whose endothelial integrity has been interrupted, though it has become increasingly clear that platelets have important roles in maintaining vascular integrity and the inflammatory response. (oncohemakey.com)
- For example, Bernard-Soulier syndrome (BSS) results from defects in the platelet receptor glycoprotein Ibα/Ibβ/IX/V (GPIb/IX) complex, which binds von Willebrand factor (VWF) and is critical for adherence of platelets at sites of vascular injury. (oncohemakey.com)
- Sensing abnormalities in their microenvironment, adhering to damaged vascular walls, and aggregating to each other are central functions of circulating platelets. (oncohemakey.com)
Cocultured endothelial cells1
- Lentivirus vector‑interleukin‑10 green fluorescent protein (LV‑IL‑10‑GFP) was transfected into endothelial progenitor cells (EPCs) in the present study. (spandidos-publications.com)
- Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play a crucial role in neovascularazation. (spandidos-publications.com)
- Simvastatin, an HMG-CoA reductase inhibitor that is used as a cholesterol-lowering agent, promotes endothelial differentiation from epithelial progenitor cells (EPC). (mendeley.com)
- Endothelial progenitor cells (EPCs), especially late EPCs, play a critical role in endothelial maintenance and repair, and postnatal vasculogenesis. (biomedcentral.com)
- however, recent studies have demonstrated that endothelial progenitor cells could promote neovascularization, 4 which presents a paradigm in which vasculogenesis contributes to therapeutic neovascularization in the adult. (ahajournals.org)
- For example, the Transplantation Of Progenitor Cells And Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI) trial indicated that circulating endothelial progenitor cells could improve left ventricular ejection fraction in patients after myocardial infarction by improving neovascularization. (ahajournals.org)
- Transplantation of bone marrow cells as well as circulating endothelial progenitor cells (EPC) enhances neovascularization after ischemia. (ahajournals.org)
- Circulating endothelial progenitor cells (EPC) play a crucial role in postnatal neovascularization. (ahajournals.org)
- 14-16 The CXCR4 receptor, which is highly expressed on both endothelial and hematopoietic progenitor cells, 17,18 has been shown to be essentially involved in mobilization and homing of hematopoietic stem cells, 19,20 and CXCR4-dependent migration toward stromal cell-derived factor-1 (SDF-1) correlated with stem cell function. (ahajournals.org)
- A growing body of evidence suggests the contribution of bone marrow-derived endothelial progenitor cells, monocytic cells, and mature endothelial cells to vessel formation and endothelial rejuvenation. (ahajournals.org)
- However, the definition and biology, especially of endothelial progenitor cells, is complex and under heavy debate. (ahajournals.org)
- In this review, we focus on current definitions of endothelial progenitor cells, highlight the clinical relevance of endothelial-regenerating cells, and provide new insights into cell-cell interactions involved in endothelial cell rejuvenation. (ahajournals.org)
- 2-6 The depiction of circulating endothelial progenitor cells (EPCs) in adults has substantially extended hypotheses about postnatal endothelial biology. (ahajournals.org)
- We investigated, as one such repair factor, the T. cruzi parasite-derived neurotrophic factor (PDNF), known to trigger survival of cardiac myocytes and fibroblasts and upregulate chemokine chemokine C-C motif ligand 2, which promotes migration of regenerative cardiac progenitor cells (CPCs). (aspetjournals.org)
- Simultaneous isolation of rare endothelial cells or endothelial progenitor cells and MSCs from WJ could explain these results. (biomedcentral.com)
- Pigment epithelium-derived factor (PEDF) peptide promotes the expansion of hepatic stem/progenitor cells via ERK and STAT3-dependent signaling. (abcam.com)
Involved in endothelial cell1
- The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. (curehunter.com)
- Eyrythropoietin, a glycoprotein growth hormone secreted by renal juxtaglomerular cells in response to reduced oxygen tension, plays a critical role in the homeostatic regulation of red cell mass. 1 , 2 Erythropoietin promotes red blood cell maturation in the bone marrow by binding to homodimeric receptors coupled to anti-apoptotic Akt and JAK-STAT signaling pathways in erythroid precursors. (pubmedcentralcanada.ca)
- Erythropoietin receptors are also expressed in non-hematopoietic tissues, including human adult myocardium and vascular endothelial cells. (pubmedcentralcanada.ca)
- Changes of messenger RNA expression of angiogenic factors and related receptors during follicular development in gilts. (semanticscholar.org)
Promoting vascular permeability1
- In vivo, more lanreotide-PM accumulated in SSTR2 high expression tumor xenografts, endocytosed by the tumor cells, induced more apoptosis of tumor cells, and suppressed tumor growth efficiently. (jove.com)
- Hyperoxia causes decreased expression of vascular endothelial growth factor and endothelial cell apoptosis in adult retina. (semanticscholar.org)
- In addition, LPS exposure caused apoptosis and suppressed cellular growth and induced P-selectin expression. (jimmunol.org)
- NF-κB signaling through IκBα degradation resulted in MnSOD upregulation and preserved cell growth, whereas NF-κB signaling through IκBβ degradation mediated apoptosis and P-selectin expression. (jimmunol.org)
- In vivo cell proliferation, apoptosis, and vasculature in tumors were monitored by Ki-67 immunostaining, TUNEL assay, and von Willebrand factor immunostaining, respectively. (beds.ac.uk)
- Similar to other vascular pathology in SSc, SSc-PAH vascular remodeling consists of intimal thickening of pulmonary arterioles and capillaries due to intimal cell proliferation and deposition of extracellular matrix . (pubmedcentralcanada.ca)
- Proliferation of endothelial cells. (miltenyibiotec.com)
- Kaposi's Sarcoma (KS) is a proliferation of aberrant vascular structures lined by spindle cells, and is caused by a gammaherpes virus (HHV8/KSHV). (biomedcentral.com)
- KS lesions have a complex histology, are localized to the dermis, and are characterized by a proliferation of aberrant vascular structures lined by spindle cells. (biomedcentral.com)
- To study blood-retina barrier (BRB) regulation, we sought to establish neuronal microvascular endothelial cells (ECs) with expanded life span by ectopic expression of the human telomerase gene (hTERT). (arvojournals.org)
- Primary cultures of human brain and bovine retinal microvascular endothelial cells (HBECs and BRECs, respectively) were transfected with the catalytic component of human telomerase human telomerase reverse transcriptase (hTERT), and colonies were selected with puromycin. (arvojournals.org)
- However, all co-cultures established so far have utilized immortalized ARPE19 cells and RF-6A or dermal microvascular endothelial cells. (biomedcentral.com)
- These findings suggest the possibility that the cerebral microvasculature may be a specialized region of the vascular system in which urokinase-type plasminogen activator, not tissue-type plasminogen activator, is the key catalyst of fibrin lysis when the brain responds to thrombotic events and that α-thrombin may regulate repair of the cerebral microvascular system. (ahajournals.org)
- In addition to its roles in blood coagulation, α-thrombin modulates the large-vessel-derived endothelial cell (EC) expression of numerous components, including the fibrinolytic proteins tissue-type plasminogen activator (TPA), urokinase-type plasminogen activator (UPA), and plasminogen activator inhibitor-1 (PAI-1). (ahajournals.org)
- The release of vasoactive substances, such as stromal cell-derived factor-1 (SDF-1), nitric oxide (NO), prostaglandin I 2 (PGI 2 ), plasminogen activator inhibitor-1 (PAI-1), tissue plasminogen activator (tPA), and in addition the activity of superoxide dismutase (SOD), were evaluated by ELISA. (biomedcentral.com)
- Angiosarcoma is a rare vascular-derived malignancy that accounts for approximately 1.6 percent of soft tissue sarcomas . (lymphedemapeople.com)
- Here, we demonstrate that induced endothelial-specific depletion of the transcription factor Forkhead Box O1 (FoxO1) in male mice led to increased vascular density in adipose tissue. (elifesciences.org)
- Upon high-fat diet feeding, endothelial cell FoxO1-deficient mice exhibited even greater vascular remodeling in the visceral adipose depot, which was paralleled with a healthier adipose tissue expansion, higher glucose tolerance and lower fasting glycemia concomitant with enhanced lactate levels. (elifesciences.org)
- Mechanistically, FoxO1 depletion increased endothelial proliferative and glycolytic capacities by upregulating the expression of glycolytic markers, which may account for the improvements at the tissue level ultimately impacting whole-body glucose metabolism. (elifesciences.org)
- Connective Tissue Growth Factor Mediates Transforming Growth Factor β-Induced Collagen Synthesis: Down-Regulation by cAMP," FASEB J. (1999) 13:1774-1786. (freepatentsonline.com)
- Connective Tissue Growth Factor and IGF-1 are Produced by Human Renal Fibroblasts and Cooperate in the Induction of Collagen Production by High Glucose," Diabetes (2003) 52:2975-2983. (freepatentsonline.com)
- During development, endothelial cells (EC) emerge from mesodermal tissue residing in blood islands in the yolk sac or other atypical regions, like the placenta. (ahajournals.org)
- Some of these stem cells also participate in the remodeling of vascular grafts, microvessel regeneration, and formation of fibrotic tissue around biomaterial implants. (thno.org)
- Currently, treatment of atherosclerosis includes reducing risk factors such as treatment of hypercholesterolemia and hypertension [ 1 , 2 ] and, for advanced disease, surgery such as stent implantation and bypass surgery using autologous vessels or tissue-engineered vascular grafts [ 5 ]. (thno.org)
- For growth anddevelopment of tissue engineered tooth, stem cells areimplanted into different tissues that need suitable growingenvironment in vivo (Figure 1). (dokumen.tips)
- Moreover, these tumors evidenced highly peculiar nest-like shapes harboring both vascular and cancerous tissue structures, which expressed the blood vessel specific marker, the von Willebrand factor. (biomedcentral.com)
- Prognostic value of interleukin-6, plasma viscosity, fibrinogen, von Willebrand factor, tissue factor and vascular endothelial growth factor levels in congestive heart failure. (qxmd.com)
- Pathologic changes seen on autopsy lack major cell and tissue injury but include signs of pleural effusion, pulmonary edema, ascites, and gastrointestinal mucosa bleeding ( 5 , 6 ), all indications of systemic vascular leakage. (cdc.gov)
- This review provides an overview and a comparison of the current knowledge of co-cultures of human endothelial cells (ECs) with human adipose tissue-derived stem/stromal cells (ASCs) or bone marrow-mesenchymal stem cells (BMSCs) in three dimensional (3D) hydrogel matrices. (springeropen.com)
- One of the major challenges in tissue engineering today is the realization of an integrated vascular network to provide adequate blood supply for living cells in tissue constructs. (springeropen.com)
- Von Willebrand factor is a marker of platelet and endothelial activation. (wikipedia.org)
- Indeed, endothelial cell marker mRNAs (very similar to that seen in human endothelial cells derived from the umbilical cord vein) are found in the cell suspension upon isolation but before culture, although these are not detected as proteins. (biomedcentral.com)
Cells and megakaryocytes1
- Hypoxia-inducible factor-1 (HIF) is a transcription factor central to oxygen homeostasis. (pnas.org)
- The transcription factor hypoxia-inducible factor-1 (HIF) is a master regulator of the hypoxic response, controlling genes involved in diverse processes that balance metabolic supply and demand within tissues ( 3 , 4 ), making modulation of HIF activity an attractive approach for the treatment of ischemic disease. (pnas.org)
- In pigs, 3 months after transplantation of kidney grafts, we observed a deregulation of the hypoxia inducible factor 1a-vascular endothelial growth factor-A axis induced in cholesterol-enriched diet animals concomitant with an overexpression of thrombospondin-1 and a decrease in cortical microvessel density promoting vascular remodeling. (springer.com)
- Supporting these in vivo findings, LPS induced NF-κB activation and MnSOD expression in isolated fetal pulmonary arterial endothelial cells. (jimmunol.org)
- Integration of signaling molecules in scaffolds to stimulate the growth of blood vessels from the host after in vivo implantation is a currently pursued strategy. (springeropen.com)
- Significant differences in expression of nerve growth factor existed in skin, and the differences were associated with the intensity of nerve injury. (thejns.org)
- We hypothesized that hypercholesterolemia could affect vascular repair processes and promote post-transplant renal vascular remodeling through the over-expression of the anti-angiogenic thrombospondin-1 interacting with vascular endothelial growth factor-A levels. (springer.com)
- The observed pattern of angiogenin expression is compatible with a role in blood vessel formation and in cross-talk between trophoblasts and endothelial cells. (sigmaaldrich.com)
- In avian and rodent species, endothelial progenitors are closely connected to the hematopoietic lineage. (ahajournals.org)
- Flk-1/KDR null mutant mice exhibited impaired endothelial and hematopoietic cell development. (pnas.org)
- The flk-1/KDR −/− homozygous mice showed a severe deficiency in vascular formation in association with hematopoietic impairment resulting in death at embryonic day (E) 8.5 ( 7 ). (pnas.org)
- von Willebrand factor (vWF) is a major procoagulant molecule that was shown to differentiate between metastatic and primary osteosarcoma (OS) tissues and associated with increased metastasis. (bioscirep.org)
- 5 Recent studies have demonstrated that embryonic stem cells (ES cells) can also differentiate into functional endothelial cells (ES-ECs) through the vasculogenesis process. (ahajournals.org)
- The ability of MSCs to differentiate into endothelial cells remains controversial. (biomedcentral.com)
- It would be very interesting to know whether the mesenchymal-endothelial like cells described in this study maintain their capacity to differentiate into adipocytes, osteocytes and chondrocytes. (biomedcentral.com)
Epithelial and endothelial1
- More banal tumors are comprised of clonal proliferations of endothelial cells that line blood vessels and lymphatics. (statpearls.com)
- Understanding the contribution of vascular cells to blood vessel remodeling is critical for the development of new therapeutic approaches to cure cardiovascular diseases (CVDs) and regenerate blood vessels. (thno.org)
- VSCs or similar stem cells in mesenchymal tissues, for instance, also participate in the regeneration of blood vessels following the implantation of vascular grafts. (thno.org)
- On the other hand, Flt-1 null mutation resulted in early embryonic death at embryonic day 8.5, showing disorganization of blood vessels, such as overgrowth of endothelial cells. (pnas.org)
- On the other hand, the flt-1 −/− homozygotes showed a disorganization of blood vessels, particularly an overgrowth of endothelial-like abnormal cells within vascular lumens and died at E8.5 to E9.0 ( 8 ). (pnas.org)
- Cutaneous angiosarcoma is an uncommon malignant tumor of vascular endothelial cells that most often affects the elderly and has a very poor prognosis. (statpearls.com)
- Tumor histology varies widely and ranges from well-differentiated variants, appearing very similar to benign vascular neoplasms, to poorly differentiated neoplasms that are difficult to discern from other malignant neoplasms. (statpearls.com)
- To assess the effect of Celastrus orbiculatus (COE) on growth, invasion and migration of human gastric cancer MGC-803 cells and to explore the possible mechanism. (jove.com)
- The present study was undertaken to test the ability of 16K hPRL to inhibit the growth of human HCT116 colon cancer cells transplanted s.c. into Rag1 −/− mice. (aacrjournals.org)
- Both bovine retinal and human brain microvascular ECs expressing hTERT resembled young primary ECs in their morphology and growth response after more than 100 population doublings. (arvojournals.org)
- We have isolated primary retinal pigment epithelial cells (hRPE) and human primary choroidal endothelial cells (hCEC) from donor eyes to construct a bilayer of hCEC/hRPE on transwell inserts. (biomedcentral.com)
- Placental Stem Villus Arterial Remodeling Associated with Reduced Hydrogen Sulfide Synthesis Contributes to Human Fetal Growth Restriction. (abcam.com)
- The most active, RERF, prevents tube formation by human endothelial cells exposed to SRSRY. (aacrjournals.org)
- In contrast, treatment with bovine pancreatic RNase A or human recombinant RNase1 interfered with leukocyte recruitment and collateral artery growth. (bloodjournal.org)
- Moreover, infection, vascular puncture, and hemostasis may be related to the increase in serum Cu/Zn-SOD levels. (scirp.org)
- The von Willebrand factor: Ag/ADAMTS13:AC ratio was exclusively correlated with tumor volume and stage as well as serum vascular endothelial growth factor levels. (biomedcentral.com)
- Gelatin zymography Growth trained moderate was ready as comes after: after 36 l of model, 100 MOI of either Ad-MMP-2-Si buy 57-22-7 or Ad-SV disease, moderate was eliminated from A549 cells, cleaned with PBS, 3 mL of serum free of charge moderate was added, and cells had been incubated for another 12 l. (neuroart2006.com)