The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Microscopy, Atomic Force
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
Microscopy, Electron, Transmission
Microscopy, Fluorescence, Multiphoton
Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
Microscopy, Scanning Tunneling
A type of scanning probe microscopy in which a very sharp conducting needle is swept just a few angstroms above the surface of a sample. The tiny tunneling current that flows between the sample and the needle tip is measured, and from this are produced three-dimensional topographs. Due to the poor electron conductivity of most biological samples, thin metal coatings are deposited on the sample.
The science and application of a double-beam transmission interference microscope in which the illuminating light beam is split into two paths. One beam passes through the specimen while the other beam reflects off a reference mirror before joining and interfering with the other. The observed optical path difference between the two beams can be measured and used to discriminate minute differences in thickness and refraction of non-stained transparent specimens, such as living cells in culture.
Microscopy, Electron, Scanning Transmission
A type of TRANSMISSION ELECTRON MICROSCOPY in which the object is examined directly by an extremely narrow electron beam scanning the specimen point-by-point and using the reactions of the electrons that are transmitted through the specimen to create the image. It should not be confused with SCANNING ELECTRON MICROSCOPY.
Microscopy, Scanning Probe
Scanning microscopy in which a very sharp probe is employed in close proximity to a surface, exploiting a particular surface-related property. When this property is local topography, the method is atomic force microscopy (MICROSCOPY, ATOMIC FORCE), and when it is local conductivity, the method is scanning tunneling microscopy (MICROSCOPY, SCANNING TUNNELING).
A scientific tool based on ULTRASONOGRAPHY and used not only for the observation of microstructure in metalwork but also in living tissue. In biomedical application, the acoustic propagation speed in normal and abnormal tissues can be quantified to distinguish their tissue elasticity and other properties.
Staining and Labeling
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Green Fluorescent Proteins
Image Processing, Computer-Assisted
The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object.
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Recirculating, dendritic, antigen-presenting cells containing characteristic racket-shaped granules (Birbeck granules). They are found principally in the stratum spinosum of the EPIDERMIS and are rich in Class II MAJOR HISTOCOMPATIBILITY COMPLEX molecules. Langerhans cells were the first dendritic cell to be described and have been a model of study for other dendritic cells (DCs), especially other migrating DCs such as dermal DCs and INTERSTITIAL DENDRITIC CELLS.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Efron, Nathan; Al-Dossari, Munira; Pritchard, Nicola (2009-05-01). "In vivo confocal microscopy of the bulbar conjunctiva". ... Ditzel, Jørn (1967-01-12). "The in Vivo Reactions of the Small Blood Vessels to Diabetes Mellitus". Acta Medica Scandinavica. ... MacKenzie, L. E.; Choudhary, T. R.; McNaught, A. I.; Harvey, A. R. (2016-06-15). "In vivo oximetry of human bulbar conjunctival ... it is possible to see groups of individual red blood cells flowing in vivo. Fundus cameras may also be used for low- ...
April 2013). "Rapid Image Evaluation System for Corneal In Vivo Confocal Microscopy" (PDF). Cornea. 32 (4): 460-465. doi: ... 9th conference of the French Microscopy Society (French: Société Française des Microscopies) (SFµ) (in French). Retrieved 25 ... Image analysis is also used in histology to study angiogenesis in 2D and 3D on microscopy images to measure effects of ... Ophthalmology Confocal image of the cornea nerves. Ophthalmology Immune-marqued cells detection. Oncology Selection of ...
"In Vivo Confocal Microscopy in a Patient with Keratopigmentation (Corneal Tattooing)." Cornea 2005; 24:2:238-240. Panda, A. et ...
"Perfluorodecalin enhances in vivo confocal microscopy resolution of Arabidopsis thaliana mesophyll" (PDF). New Phytologist. 186 ... Perfluorodecalin has also been shown to dramatically enhance in vivo microscopy resolution of airspace-containing tissues such ...
Effects of long-term contact lens wear on the cornea
Patel, S.; McLaren, J.; Hodge, D.; Bourne, W. (April 2002). "Confocal Microscopy In Vivo in Corneas of Long-Term Contact Lens ...
Polglase, A.L., W.J. Mclaren, and S.A. Skinner, A fluorescence confocal endomicroscope for in vivo microscopy of the upper- and ... It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have ... Journal of microscopy, 2011. 242: p. 111-6. Glass, Monty; Dabbs, Tim (1992-02-20). "Single-mode fibers used as confocal ... 189: p. 111-9. Sonn, G.a., et al., Optical biopsy of human bladder neoplasia with in vivo confocal laser endomicroscopy. The ...
"Normal human keratocyte density and corneal thickness measurement by using confocal microscopy in vivo". Invest. Ophthalmol. ...
Owl's eye appearance
"Mapping owl's eye cells of patients with cytomegalovirus corneal endotheliitis using in vivo laser confocal microscopy". ...
Live cell imaging
Keller HE (2006), "Objective Lenses for Confocal Microscopy", Handbook of Biological Confocal Microscopy, Springer US, pp. 145- ... Petroll, W. M.; Jester, J. V.; Cavanagh, H. D. (May 1994). "In vivo confocal imaging: general principles and applications". ... The rise of confocal microscopy is closely correlated with accessibility of high power lasers, which are able to achieve high ... Hibbs AR (2004). Confocal microscopy for biologists. New York: Kluwer Academic/Plenum Publishers. ISBN 978-0306484681. OCLC ...
... a prospective study using in vivo confocal microscopy". Invest Ophthalmol Vis Sci. 50 (9): 4192-8. doi:10.1167/iovs.09-3781. ...
Other modalities, such as corneal thickness measurement (pachymetry), in-vivo confocal biomicroscopy, and specular microscopy ... Scheimpflug imaging, anterior segment optical coherence tomography, confocal microscopy, and specular microscopy are additional ...
Fluorescence correlation spectroscopy
Commonly, FCS is employed in the context of optical microscopy, in particular Confocal microscopy or two-photon excitation ... This opens a new area, "in situ or in vivo biochemistry": tracing the biochemical pathway in intact cells and organs. ... a number of improvements in the measurement techniques-notably using confocal microscopy, and then two-photon microscopy-to ... Diaspro, A.; Robello, M. (1999). "Multi-photon Excitation Microscopy to Study Biosystems". European Microscopy and Analysis. 5 ...
Efron, Nathan; Al-Dossari, Munira; Pritchard, Nicola (1. 5. 2009). "In vivo confocal microscopy of the bulbar conjunctiva". ... 1. 1967). "The in Vivo Reactions of the Small Blood Vessels to Diabetes Mellitus". Acta Medica Scandinavica (jezik: engleski). ... sistemima za snimanje pod velikim povećanjem moguće je vidjeti grupe pojedinačnih crvenih krvnih zrnaca kako teku in vivo. ... "Dynamics of external ocular blood flow studied by scanning angiographic microscopy". Eye (London, England). 9 (5): 605-614. ...
കൺജങ്റ്റൈവ - വിക്കിപീഡിയ
Efron, Nathan; Al-Dossari, Munira; Pritchard, Nicola (2009-05-01). "In vivo confocal microscopy of the bulbar conjunctiva". ... Ditzel, Jørn (1967-01-12). "The in Vivo Reactions of the Small Blood Vessels to Diabetes Mellitus". Acta Medica Scandinavica ( ...
Confocal microscopy is a non-invasive technique that allows visualization of Acanthamoeba in vivo in cases in which corneal ... Nakano E, Oliveira M, Portellinha W, de Freitas D, Nakano K (September 2004). "Confocal microscopy in early diagnosis of ...
Seok-Hyun "Andy" Yun
CS1 maint: discouraged parameter (link) Scarcelli, G; Yun, SH (2008). "Confocal Brillouin microscopy for three-dimensional ... 2006). "Comprehensive volumetric optical microscopy in vivo". Nature Medicine. 12: 1429-1433. doi:10.1038/nm1450. PMC 2709216. ... His research also contributed to the development of Brillouin microscopy and various implantable optical devices. Yun, SH; Kwok ... 2013). "Light-guiding hydrogels for cell-based sensing and optogenetic synthesis in vivo". Nature Photonics. 7: 987-994. doi: ...
Other methods to determine whether NP are present on the surface of the cell are confocal microscopy, Prussian blue ... Flynn, E R; Bryant, H C (2005-03-21). "A biomagnetic system for in vivo cancer imaging". Physics in Medicine and Biology. 50 (6 ... The conjugated NP+Ab are then incubated with the cell lines and may be examined by transmission-electron microscopy (TEM) to ...
Confocal microscopy and fluorescence microscopy of in vivo tissue Diffuse reflectance infrared fourier transform for in vivo ...
Werner Reichardt Centre for Integrative Neuroscience
... fluorescence microscopy, most importantly making use of confocal or 2-photon microscopes. The most recent innovation in optical ... numerous optical methods are employed at the CIN to make individual cells or cell clusters visible in vitro or in vivo, and to ... Super resolution microscopy can reach resolutions below the Abbe limit of conventional light microscopy. This combined method ... methods is the localisation of protein molecules in neuronal compartments by combining super-resolution microscopy with a ...
National Institute of Nutrition, Hyderabad
Confocal laser scanning microscopy, Transmission electron microscopy, DNA sequencers, Cryogenic equipment, thermal cyclers etc ... The institute is equipped with facilities among Animal housing, In vivo-imaging, Automated electrophoresis, Flow cytometry, DNA ... in vitro and in vivo". Ebm.rsmjournals.com. 4 April 2011. Retrieved 30 August 2012. Cite journal requires ,journal= (help)CS1 ...
Cadmium-free quantum dot
For example, various techniques are used for the Cd-free QDs imaging including confocal/multiphoton microscopy, CARS imaging. ... and quantum dots for in vivo biological labeling. In vivo labeling, it important for absorption and emission to occur in the ... "In vivo biodistribution studies and ex vivo lymph node imaging using heavy metal-free quantum dots". Biomaterials. 104: 182-91 ... types that have been demonstrated their low-toxicity and high colloidal and PL stability for in vitro and in vivo models. DNA/ ...
Patel DV, McGhee CN (2007). "Contemporary in vivo confocal microscopy of the living human cornea using white light and laser ... Play media Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy ... "Confocal Microscopy. The Development of a Modern Microscopy". Imaging & Microscopy.online Barry R. Masters: Confocal Microscopy ... Foundations of Confocal Scanned Imaging in Light Microscopy". In James Pawley (ed.). Handbook of Biological Confocal Microscopy ...
Hollingsworth JG, Efron N (June 2004). "Confocal microscopy of the corneas of long-term rigid contact lens wearers". Cont Lens ... Zhivov A, Stave J, Vollmar B, Guthoff R (January 2007). "In vivo confocal microscopic evaluation of langerhans cell density and ...
Patel DV, McGhee CN (2007). "Contemporary in vivo confocal microscopy of the living human cornea using white light and laser ... The Development of a Modern Microscopy". Imaging & Microscopy.. online. *^ a b c Barry R. Masters: Confocal Microscopy And ... Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing ... Foundations of Confocal Scanned Imaging in Light Microscopy". In James Pawley. Handbook of Biological Confocal Microscopy (3. ...
Lattice light-sheet microscopy
Lattice light sheet microscopy is useful for in-vivo cellular localization and super resolution. Lattice light sheets' confined ... and two orders of magnitude faster than Spinning Disk Confocal Microscopy. These two advantages combine to allow researchers to ... and Super-resolution microscopy (specifically structured illumination microscopy, SIM). In lattice light sheet microscopy, very ... Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition ...
In migrating vascular cells, T-cadherin was located at the leading edge as revealed by confocal microscopy. The distribution of ... In vivo T-cadherin was detected on the apical cell surface of the chick intestinal epithelium. In cultures of transfected MDCS ...
Optical coherence tomography
Confocal microscopy, another optical technique, typically penetrates less deeply into the sample but with higher resolution. ... First in vivo OCT images - displaying retinal structures - were published in 1993 and first endoscopic images in 1997. OCT has ... Medical ultrasonography, magnetic resonance imaging (MRI), confocal microscopy, and OCT are differently suited to morphological ... A first two-dimensional in vivo depiction of a human eye fundus along a horizontal meridian based on white light ...
Raman microscopy, and in particular confocal microscopy, has very high spatial resolution. For example, the lateral and depth ... Consequently, in vivo time- and space-resolved Raman spectroscopy is suitable to examine proteins, cells and organs. In the ... Raman microscopy for biological and medical specimens generally uses near-infrared (NIR) lasers (785 nm diodes and 1064 nm Nd: ... Raman microscopy of inorganic specimens, such as rocks and ceramics and polymers, can use a broader range of excitation ...
... brain slices are imaged with confocal microscopy. When exposed to a photon with its particular excitation wavelength, each ... Brainbow is implemented in vivo by crossing two transgenic organism strains: one that expresses the Cre protein and another ... This issue is somewhat avoided by the use of synaptic markers to supplement the use of optical microscopy in viewing synaptic ... By revealing such detailed information about neuronal connectivity and patterns, sometimes even in vivo, scientists are often ...
In confocal Raman microscopy, the diameter of the confocal aperture is an additional factor. As a rule of thumb, the lateral ... Consequently, in vivo time- and space-resolved Raman spectroscopy is suitable to examine proteins, cells and organs. In the ... Raman microscopy, and in particular confocal microscopy, can reach down to sub-micrometer lateral spatial resolution. Because a ... Confocal Raman microscopy can be combined with numerous other microscopy techniques. By using different methods and correlating ...
Detection of cholangiocarcinoma in vivo using miniprobe-based confocal fluorescence microscopy. - PubMed - NCBI
Detection of cholangiocarcinoma in vivo using miniprobe-based confocal fluorescence microscopy.. Meining A1, Frimberger E, ... Miniprobe-based confocal laser scanning microscopy considerably increases sensitivity for the detection of biliary neoplasia ... Presence of irregular vessels use confocal laser microscopy enabled prediction of neoplasia with an accuracy rate of 86%, ... To overcome this limitation, a new imaging modality was evaluated to detect neoplasia in vivo in the biliary tract. ...
Nevomelanocytic atypia detection by in vivo reflectance confocal microscopy.
However, the possibility to detect melanocytic atypia in nevi by means of in vivo RCM remains unknown. The aim of ... In vivo reflectance confocal microscopy (RCM) is a promising novel technology for non-invasive early diagnostics of cutaneous ... BACKGROUND AND OBJECTIVE: In vivo reflectance confocal microscopy (RCM) is a promising novel technology for non-invasive early ... in vivo RCM and routine histopathology. In vivo RCM images were analyzed for the arrangement of keratinocytes in epidermis, ...
Scanning slit confocal microscopy of the |i|in vivo|/i| cornea
Barry R. Masters, "Scanning slit confocal microscopy of the in vivo cornea," Opt. Eng. 34(3) (1 March 1995) Include:. ... In-vivo real-time confocal microscopy of the human cornea Proceedings of SPIE (June 24 1993) ... Barry R. Masters, "Scanning slit confocal microscopy of the in vivo cornea," Optical Engineering 34(3), (1 March 1995). https ... Finally, a real-time, scanning slit confocal microscope is described and demonstrated for obtaining 2-D images of the in vivo ...
In Vivo Confocal Microscopy of Cutaneous Neoplasms and Normal Skin - No Study Results Posted - ClinicalTrials.gov
Effects of Long-Term Antiglaucoma Eye Drops on Conjunctival Structures: An In Vivo Confocal Microscopy Study
"In vivo laser scanning confocal microscopy of the ocular surface in glaucoma," Microscopy and Microanalysis, vol. 27, pp. 1-16 ... W. Zhu, J. Hong, T. Zheng, Q. Le, J. Xu, and X. Sun, "Age-related changes of human conjunctiva on in vivo confocal microscopy ... Effects of Long-Term Antiglaucoma Eye Drops on Conjunctival Structures: An In Vivo Confocal Microscopy Study. Wenqing Zhu,1 ... Figure 1: Laser scanning in vivo confocal microscopy of the human conjunctiva in glaucoma patients and control group. (Letter A ...
NIOSHTIC-2 Publications Search - 20029365 - Sensitivity and specificity of reflectance-mode confocal microscopy for in vivo...
Reflectance-mode confocal microscopy (RCM) offers noninvasive high-resolution imaging of human skin in vivo. OBJECTIVE: The ... Reflectance-mode confocal microscopy (RCM) offers noninvasive high-resolution imaging of human skin in vivo. OBJECTIVE: The ... Sensitivity and specificity of reflectance-mode confocal microscopy for in vivo diagnosis of basal cell carcinoma: a ... Based on a previous study, a set of 5 histologically correlated confocal imaging criteria for diagnosing BCC was established, ...
In Vivo Reflectance Confocal Microscopy of Skin
Clinical in vivo confocal microscopy of the human cornea in health and disease
Confocal microscopy enables microstructural analysis of the in vivo cornea, allowing fresh insight into corneal microstructure ... Clinical in vivo confocal microscopy of the human cornea in health and disease Prog Retin Eye Res. 2010 Jan;29(1):30-58. doi: ... Confocal microscopy enables microstructural analysis of the in vivo cornea, allowing fresh insight into corneal microstructure ... This article reviews in vivo confocal microscopy of the human cornea in health and disease and examines clinical and research ...
Small fiber neuropathy in women with fibromyalgia. An in vivo assessment using corneal confocal bio-microscopy. - PubMed - NCBI
An in vivo assessment using corneal confocal bio-microscopy.. Ramírez M1, Martínez-Martínez LA2, Hernández-Quintela E1, Velazco ... Corneal confocal bio-microscopy is a new noninvasive method to evaluate small nerve fiber morphology. Our objective was to ... Corneal confocal microscopy could become a useful test in the study of patients with fibromyalgia. ... Corneal confocal microscopy; Dorsal root ganglia; Fibromyalgia; Neuropathic pain; Small fiber neuropathy; Sympathetic pain ...
In vivo confocal microscopy in fungal keratitis | British Journal of Ophthalmology
In vivo confocal microscopy in fungal keratitis | British Journal of Ophthalmology
Pigmented Nodular Basal Cell Carcinomas in Differential Diagnosis with Nodular Melanomas: Confocal Microscopy as a Reliable...
Reflectance confocal microscopy (RCM) in an emerging noninvasive diagnostic tool that provides in vivo tissue images at nearly ... Reflectance confocal microscopy is a relatively new imaging technique that permits to evaluate in vivo skin tumors with a ... S. González and Z. Tannous, "Real-time, in vivo confocal reflectance microscopy of basal cell carcinoma," Journal of the ... P. Guitera, G. Pellacani, K. A. Crotty et al., "The impact of in vivo reflectance confocal microscopy on the diagnostic ...
Thygeson Superficial Punctate Keratitis: An Evaluation With in vivo Confocal Microscopy | IOVS | ARVO Journals
Thygeson Superficial Punctate Keratitis: An Evaluation With in vivo Confocal Microscopy N. Amar; A. Labbe; C. Baudouin; S. Doan ... In vivo confocal microscopy images showed in the superficial epithelium round and/or oval shaped zones of hyper-reflective and ... Thus in vivo confocal microscopy revealed epithelial and stromal changes compatible with an inflammatory involvement. ... Mean age was 30.2 years (ranged from 5 to 68 years). Slit-lamp biomicroscopy and in vivo confocal microscopy with HRT II/ ...
In vivo imaging of Posterior Polymorphous Corneal Dystrophy using Confocal Microscopy | IOVS | ARVO Journals
In vivo imaging of Posterior Polymorphous Corneal Dystrophy using Confocal Microscopy You will receive an email whenever this ... Lucia Lapenna, Antonio Acquaviva; In vivo imaging of Posterior Polymorphous Corneal Dystrophy using Confocal Microscopy. Invest ... as imaged with laser scanning in vivo confocal microscopy (IVCM). Methods : IVCM images of 3 subjects with PPCD who had typical ...
In Vivo Confocal Microscopy: A New Possibility to Confirm the Diagnosis of Borrelia Keratitis?
Molecular Vision: In vivo observation of Langerhans cells by laser confocal microscopy in Thygeson's superficial punctate...
In vivo imaging of eyes affected by TSPK has been performed by confocal microscopy . The images revealed highly refractive ... In vivo confocal microscopy for studying corneal diseases and conditions associated with corneal nerve damage. Adv Exp Med Biol ... In vivo observation of Langerhans cells by laser confocal microscopy in Thygesons superficial punctate keratitis. Koji ... In contrast to in vivo tandem scanning confocal microscopy, the HRTII-RCM is able to provide high-resolution images of each ...
i|In vivo|/i| fluorescence confocal microscopy: indocyanine green enhances the contrast of epidermal and dermal structures
Of particular importance is the use of reflectance confocal microscopy (RCM) and fluorescence confocal microscopy (FCM) that ... In vivo confocal microscopy is being widely established as a useful investigative tool in skin research.1, 2 The aims of in ... In vivo confocal microscopy offers real-time scan sequences with gray-scaled images obtained in horizontal (en face) view to ... M. Rajadhyaksha, S. Gonzalez, J. M. Zavislan, R. R. Anderson, and R. H. Webb, "In vivo confocal scanning laser microscopy of ...
The appearance of hyper-reflective superficial epithelial cells observed using in vivo confocal microscopy
Letter: In vivo confocal microscopy visualization of presumed lymph vessels in a case of corneal transplant rejection
... ... The method involved in vivo examination of the cornea and subsequent comparison of in vivo findings with ex vivo ... In conclusion, we introduce a new in vivo technique for investigating angiogenesis in a corneal model were in vivo findings can ... There exist a number of models to study angiogenesis, both in vitro and in vivo, but there is no single perfect model so far. ...
In vivo confocal microscopy of the cornea to assess tissue regenerative response after biomaterial implantation in humans.
Use of Confocal Microscopy to Evaluate Equine Zygote Development After Sperm Injection of Oocytes Matured In Vivo or In Vitro
... of oocytes that were matured in vivo or in vitro. After fixation for 4, 6, 8, 12, or 16 h after ICSI, zygotes were incubated ... Confocal microscopy was used to image stages of equine zygote development, at timed intervals, after intracytoplasmic sperm ... Use of Confocal Microscopy to Evaluate Equine Zygote Development After Sperm Injection of Oocytes Matured In Vivo or In Vitro ... Confocal microscopy was used to image stages of equine zygote development, at timed intervals, after intracytoplasmic sperm ...
In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure -...
In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure ... We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to ... In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure. Optics ... "In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure," Opt. ...
OSA | In vivo quantitation of injected circulating tumor cells from great saphenous vein based on video-rate confocal microscopy
In vivo quantitation of injected circulating tumor cells from great saphenous vein based on video-rate confocal microscopy ... "In vivo quantitation of injected circulating tumor cells from great saphenous vein based on video-rate confocal microscopy," ... "In vivo analysis of THz wave irradiation induced acute inflammatory response in skin by laser-scanning confocal microscopy," ... "In vivo analysis of THz wave irradiation induced acute inflammatory response in skin by laser-scanning confocal microscopy," ...
Ex vivo confocal microscopy performs real-time assessment of renal biopsy in non-neoplastic diseases. | Physician's Weekly
The ex vivo confocal microscopy protocol did not add artifacts to the sample for the ulterior study with light microscopy, nor ... Ex vivo confocal microscopy performs real-time assessment of renal biopsy in non-neoplastic diseases.. Sep 7, 2020 ... Ex vivo confocal microscopy is a technique for tissue examination, which generates images of fresh samples with an optical ... The objective of this study was to evaluate the feasibility of using ex vivo confocal microscopy in fusion mode (reflectance ...
Diagnostic traps based on nomenclature of inflammatory changes in corneal confocal microscopy in vivo
... ... To present possible misdiagnosis of infectious keratitis in corneal confocal microscopy in vivo images based on typically used ... Retrospective analysis of in vivo confocal microscopy images from 155 patients with different diagnosis. 63 cases were ... Diagnostic process using confocal microscopy examination cannot be based just on algorithmic analysis of corneal images. For ...
Confocal microscopy to guide Erbium:yttrium aluminum garnet laser ablation of basal cell carcinoma: an |i|ex vivo|/i|...
Confocal microscopy to guide Erbium:yttrium aluminum garnet laser ablation of basal cell carcinoma: an ex vivo feasibility ... Intra-operative reflectance confocal microscopy (RCM) may provide a means to detect residual tumor directly on the patient and ... à-vis the feasibility of labeling and RCM imaging in human skin ex vivo, following ablation with an erbium:yttrium aluminum ...
Touching The Human Neuron: User-Centric Augmented Reality Viewing and Interaction of in-vivo Cellular Confocal Laser Scanning...
User-Centric Augmented Reality Viewing and Interaction of in-vivo Cellular Confocal Laser Scanning Microscopy (CLSM) Utilizing ... User-Centric Augmented Reality Viewing and Interaction of in-vivo Cellular Confocal Laser Scanning Microscopy (CLSM) Utilizing ... Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) are current methods used to visualize cellular ... Confocal laser scanning microscopy principles. Zeiss Jena http:// zeiss-campus.magnet.fsu.edu/referencelibrary/pdfs/ ...
Diagnostic accuracy of pigmented labial macules by in vivo reflectance confocal microscopy and correlation among techniques. |...
Autologous Serum Tears for Treatment of Photoallodynia in Patients with Corneal Neuropathy: Efficacy and Evaluation with In...
... in vivo confocal microscopy and neuropathy, in vivo confocal microscopy and nerves. ... Repeatability of in vivo corneal confocal microscopy to quantify corneal nerve morphology. Cornea. 2013;32:e83-9. [PubMed] ... C. In Vivo Confocal Microscopy Findings. A summary of the nerve parameters for the patients, pre- and post-treatment with AST, ... In vivo confocal microscopy of corneal stromal nerves in patients with peripheral neuropathy. Arch Neurol. 2009;66:1179-80. ...
Role of In Vivo Reflectance Confocal Microscopy in the diagnosis of a histopathologic difficult melanoma - Giornale Italiano di...
MicroscopeFluorescence confocal microscopyIVCMCorneaMETHODSDiagnosisOptical coherenceAbstractOphthalmologyLight microscopyMicroscopicVisualizationVitro and in vivoAssessmentFindingsMelanomaClinicalTissueEpithelialReflectance microscopyCharacterizationVideo-rate confocalStaining of the ocular surfaceLaser Scanning MicroscopyPrinciple of confocalHistologicalFungal keratitisFeasibilityCorneal epitheliumEndothelialOcular surfaceConjunctivalImagesConjunctivaKeratitisDisk confocalMeans of in vivoHuman skin in vivoStudyMultiphoton microscopyCONCLUSIONSExaminationFluorescentImagingPhysiologicalCellular levelIntraoperativeElectron microscopyFOCISlitEnhancesHistopathologyMurine
- The 1-D z scanning confocal microscope is described and its performance demonstrated with depth scans across the full thickness of the in vivo rabbit cornea. (spiedigitallibrary.org)
- This instrument uses an axial scanning microscope objective to scan through the full thickness of the in vivo cornea. (spiedigitallibrary.org)
- Finally, a real-time, scanning slit confocal microscope is described and demonstrated for obtaining 2-D images of the in vivo human cornea. (spiedigitallibrary.org)
- The unique imaging characteristics of this scanning slit in vivo confocal microscope is illustrated with a series of reflected light images of the normal in vivo human cornea. (spiedigitallibrary.org)
- Optical Sectioning With The Scanning Slit Confocal Microscope Applications. (spiedigitallibrary.org)
- A central corneal thickness scan was obtained with a confocal microscope. (nih.gov)
- An existing suture model for inflammatory angiogenesis in the cornea was modified for in vivo observations with a clinically-approved corneal confocal microscope. (diva-portal.org)
- An Olympus IX81 spinning disk confocal microscope was used for imaging. (nih.gov)
- We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO_2) and oxygen partial pressure (pO_2) in vivo in single blood vessels with high spatial resolution. (caltech.edu)
- The white-light confocal microscope offers an in vivo, cellular-level resolution view of the cornea. (umn.edu)
- Thus, using the confocal microscope with a fluorescent cube, we were able to visualize HSV-infected corneal epithelial cells tagged with a direct fluorescent antibody. (umn.edu)
- Purpose: To elucidate the morphological alterations of the conjunctiva in atopic keratoconjunctivitis (AKC) using the new generation Heidelberg Retina Tomograph II(HRT II)/Rostock Cornea Module confocal microscope in a prospective controlled study. (elsevier.com)
- Light travels through the sample under a conventional microscope as far into the specimen as it can penetrate, while a confocal microscope only focuses a smaller beam of light at one narrow depth level at a time. (wikipedia.org)
- In contrast, a confocal microscope uses point illumination (see Point Spread Function ) and a pinhole in an optically conjugate plane in front of the detector to eliminate out-of-focus signal - the name "confocal" stems from this configuration. (wikipedia.org)
- For the purpose, 15 children, referring at the Dental Clinic of the Second University of Naples, affected by several degrees of EDs, were enrolled and underwent in vivo RCM imaging to microscopically define the ED confocal features using a commercially available hand-held reflectance confocal microscope with neither injuries nor discomfort. (uniba.it)
- This service provides 3 PC workstations that can be used to analyze images from the microscope systems (laser and multiphton confocal, in vivo confocal, and epifluorescent). (utsouthwestern.edu)
- Recent advances in optical imaging and miniaturization have enabled the production of a hand-held intraoperative confocal microscope. (springer.com)
- We present a first-look feasibility analysis of the intraoperative confocal microscope as an adjunct for brain tumor resection. (springer.com)
- Surface tubules were observed in vivo with a 633-nm single laser-illuminated real-time video-rate confocal scanning microscope in upright configuration for optical sectioning below the renal capsule. (pubmedcentralcanada.ca)
- Cy2- or Cy3-labeled insulin uptake in a PT cell line in vitro was monitored by 488-nm excitation fluorescence microscopy using an inverted microscope. (pubmedcentralcanada.ca)
- Core instrumentation includes: an Abberior STED microscope,A Nikon A1R HD with TIRF, an Olympus FV1000 laser scanning confocal microscope, a Zeiss PALM MicroBeam Laser Capture Microdissection Microscope also used for brightfield and color imaging (H&E), and an image analysis workstation. (rochester.edu)
- This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. (springer.com)
- To address these limitations, some researchers and product developers are revamping the microscope 's fundamental optics - specifically, combining confocal techniques with other forms of microscopy such as multiphoton and optical coherence tomography. (photonics.com)
- This stack of confocal microscope images shows the distribution of actin filaments throughout a cell. (photonics.com)
- In particular, I'm talking about combining confocal fluorescence microscopy information with data from the atomic force microscope (AFM) and reflection electron microscope (REM). (photonics.com)
Fluorescence confocal microscopy3
- Of particular importance is the use of reflectance confocal microscopy (RCM) and fluorescence confocal microscopy (FCM) that enable visualization of the tissue with a resolution comparable to histology. (spiedigitallibrary.org)
- 7 Fluorescence confocal microscopy (FCM), however, relies on the different distribution of exogenous fluorescent molecules (fluorophores) that are applied to the tissue to generate a contrast. (spiedigitallibrary.org)
- Fluorescence confocal microscopy was then performed. (umn.edu)
- To Evaluate the morphological and ultrastructural findings in posterior polymorphous corneal dystrophy (PPCD) as imaged with laser scanning in vivo confocal microscopy (IVCM). (arvojournals.org)
- Laser-scanning in vivo confocal microscopy (IVCM) of the cornea is becoming an increasingly popular tool to examine the living human cornea with cellular-level detail in both healthy and pathologic states. (diva-portal.org)
- This study aimed to evaluate the efficacy of autologous serum tears (AST) for treatment of severe photoallodynia in corneal neuropathy and correlate with corneal subbasal nerve alterations by in vivo confocal microscopy (IVCM). (pubmedcentralcanada.ca)
- During follow-up, in vivo confocal microscopy (IVCM) and anterior segment optical coherence tomography (AS-OCT) was performed. (octnews.org)
- In vivo confocal microscopy (IVCM) enables microstructural analysis of the cornea. (biomedcentral.com)
- To evaluate in vivo confocal microscopy (IVCM) findings of ocular surface squamous neoplasia (OSSN) during treatment with topical interferon alfa-2b (IFN alfa-2b). (bioportfolio.com)
- Purpose This study was designed to compare and contrast quantitative data of the human corneal sub-basal nerve plexus (SBP) evaluated by two different methods: in vivo confocal microscopy (IVCM), and immunohistochemical staining of ex vivo donor corneas. (elsevier.com)
- Methods Seven parameters of the SBP in large-scale IVCM mosaicking images from healthy subjects were compared with the identical parameters in ex vivo donor corneas stained by β-III-tubulin immunohistochemistry. (elsevier.com)
- Conclusions The results of this study have shown that IVCM has an inherently lower resolution compared with ex vivo immunohistochemical staining of the corneal SBP and that this limitation leads to a systematic underestimation of several SBP parameters. (elsevier.com)
- Despite this shortcoming, IVCM is a vital clinical tool for in vivo characterization, quantitative clinical imaging, and evaluation of the human corneal SBP. (elsevier.com)
- To compare fungal culture and in vivo confocal microscopy ( IVCM ) in the diagnosis of non- primary fungal keratitis . (bvsalud.org)
- Clinical tests (IOP, Schirmer I test, and lacrimal film break-up time BUT) and in vivo conjunctival confocal microscopy (IVCM) were performed in all patients at baseline and after 12 months. (unimi.it)
- The use of fixed slit and scanning slit confocal microscopes is described and illustrated for 1-D and 2-D confocal imaging of the in vivo cornea. (spiedigitallibrary.org)
- Confocal microscopy enables microstructural analysis of the in vivo cornea, allowing fresh insight into corneal microstructure in health, and in inherited and acquired corneal disease. (nih.gov)
- This article reviews in vivo confocal microscopy of the human cornea in health and disease and examines clinical and research applications of this new technology. (nih.gov)
- Slit-lamp biomicroscopy and in vivo confocal microscopy with HRT II/ Rostock cornea module were performed. (arvojournals.org)
- To characterize the cornea of individuals with Thygeson's superficial punctate keratitis (TSPK) at the cellular level by laser confocal biomicroscopy. (molvis.org)
- The method involved in vivo examination of the cornea and subsequent comparison of in vivo findings with ex vivo immunohistochemical analysis of the same tissue samples. (diva-portal.org)
- In vivo confocal microscopy of the cornea to assess tissue regenerative response after biomaterial implantation in humans. (diva-portal.org)
- We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 x 396 µm3. (kit.edu)
- This service provides support for in vivo confocal imaging of the cornea in animal models (typically mouse or rabbit). (utsouthwestern.edu)
- Confocal microscopy allows high resolution optical sections to be obtained throughout the full thickness of the cornea. (utsouthwestern.edu)
- MATERIALS AND METHODS: A total of 138 melanocytic skin lesions comprising 25 melanocytic nevi, 69 dysplastic nevi and 44 melanomas were analyzed by means of dermoscopy, in vivo RCM and routine histopathology. (biomedsearch.com)
- Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) are current methods used to visualize cellular structures. (firstmonday.dk)
- Aim of this study is the in vivo assessment at microscopic resolution of dental surfaces affected by EDs without resorting to invasive methods such as teeth extractions, to define histological findings occurring in chromatic and/or structural EDs. (uniba.it)
- Purpose: To identify genetic mutations and study the corneal epithelium in Japanese patients with Meesmann corneal dystrophy.Design: Laboratory investigation and prospective observational case series.Methods: Slit-lamp biomicroscopy with fluorescein vital staining and in vivo confocal microscopy were performed. (linkoph.com)
- 'Smarter imaging: Gaining more from your microscopy experiments' highlights several recent studies from researchers who not only use high-throughput methods but combine them with novel engineering techniques, whether at the specimen or platform level, to gain more from their imaging experiments. (microscopyu.com)
- Information is provided on the practical and technical characteristics of a wide range of equipment and methods for in vivo measurements that aid in the investigation of skin function, the evaluation of topically applied products and the monitoring of skin disease. (springer.com)
- The aim of the study was to evaluate the significance of in vivo RCM features of melanocytic atypia for the diagnosis of melanocytic nevi, dysplastic nevi and cutaneous melanoma. (biomedsearch.com)
- RESULTS: Separately and all together taken the in vivo RCM features of melanocytic atypia were significant in differential diagnosis of benign and malignant melanocytic skin lesions, though none of the features was significant in discriminating nevi without cytologic atypia of dysplastic nevi. (biomedsearch.com)
- Sensitivity and specificity of reflectance-mode confocal microscopy for in vivo diagnosis of basal cell carcinoma: a multicenter study. (cdc.gov)
- CONCLUSION: RCM offers a sensitive and specific tool for the noninvasive diagnosis of BCC in vivo. (cdc.gov)
- Confocal microscopy has been utilised clinically to aid in the diagnosis of infectious keratitis, in particular Acanthamoeba and fungal keratitis, and has also established a role in the diagnosis and phenotyping of corneal dystrophies. (nih.gov)
- Here, we present four cases of challenging nodular lesions where confocal microscopy was able to clarify the diagnosis. (hindawi.com)
- The striking confocal features for MM diagnosis include the presence of nonedged papillae corresponding to a disarray of the rete ridge, cytological atypia at the dermal-epidermal junction, the presence of round pagetoid cells, the presence of nucleated cells corresponding to melanocytes infiltrating the dermal papillae, and the cerebriform nests in the dermis correlated to the presence of large aggregates of small melanocytes [ 5 , 6 ]. (hindawi.com)
- In Vivo Confocal Microscopy: A New Possibility to Confirm the Diagnosis of Borrelia Keratitis? (ovid.com)
- Retrospective analysis of in vivo confocal microscopy images from 155 patients with different diagnosis. (ovid.com)
- For reliable diagnosis microscopy images should be compared with clinical state and anamnesis. (ovid.com)
- Babino G, Moscarella E, Agozzino M, Alfano R, Argenziano G. Role of In Vivo Reflectance Confocal Microscopy in the diagnosis of a histopathologic difficult melanoma. (minervamedica.it)
- This process may prove to be a useful clinical tool for the in vivo diagnosis of HSV keratitis. (umn.edu)
- In vivo reflectance confocal microscopy (RCM) is a novel technique that has demonstrated high sensitivity and specificity in the in vivo diagnosis of BCC. (blogspot.com)
- and has shown utility for the diagnosis of nonmelanoma skin cancer in vivo. (dangareau.net)
- Tandem scanning confocal corneal microscopy in the diagnosis of suspected Acanthamoeba keratitis. (cdc.gov)
- Diagnosis of Acanthamoeba keratitis in vivo with confocal microscopy. (cdc.gov)
- A 15-year-old girl, who was diagnosed by slit-lamp microscopy as PCV, was examined using specular microscopy, in vivo confocal microscopy, and anterior segment OCT (optical coherence tomography). (nih.gov)
- Abstract Reflectance confocal microscopy (RCM) is a noninvasive imaging technique that allows visualization of the epidermis and papillary dermis with cellular-level resolution. (bvsalud.org)
- All patients underwent a complete ophthalmology visit including refraction with determination of ETDRS-measured Best Spectacle Corrected Visual Acuity (BSCVA), biomicroscopy, intraocular pressure measurement, and in-vivo scanning laser confocal microscopy (LCM). (biomedcentral.com)
- Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT , the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. (springer.com)
- High Resolution Imaging in Microscopy and Ophthalmology - New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. (springer.com)
- The ex vivo confocal microscopy protocol did not add artifacts to the sample for the ulterior study with light microscopy, nor to the histochemical or immunohistochemical studies. (physiciansweekly.com)
- 1992). When compared to traditional light microscopy, SEM offers a greater depth of field, allowing for more of a specimen to be in focus at one time. (firstmonday.dk)
- The URMC Center for Advanced Light Microscopy and Nanoscopy (CALMN) provides UR researchers the ability to obtain high quality imaging data using state-of-the-art microscopy instruments. (rochester.edu)
- The URMC Center for Advanced Light Microscopy and Nanoscopy (CALMN) provides UR Medical Center and UR River Campus researchers access to high-end confocal microscopy, laser capture microdissection, as well as more conventional brightfield and immunofluorescence microscopy. (rochester.edu)
- Reflectance confocal microscopy (RCM) has been proven to provide images with a microscopic resolution high enough to view cellular and sub-cellular detail. (spiedigitallibrary.org)
- Conclusions This is the first FCFM study to describe in vivo microscopic changes in the airways and alveoli of patients with COPD that are related to lung function impairment. (bmj.com)
- Changes in in vivo confocal microscopic findings of ocular surface squamous neoplasia during treatment with topical interferon alfa-2b. (bioportfolio.com)
- In vivo reflectance confocal microscopy (RCM) allows to image in vivo at microscopic resolution of the dental surface, thus avoiding the tooth extraction and the sample preparation because of its ability to optically scan living tissues along their depth. (uniba.it)
- The present in vivo microscopic study on EDs allowed to highlight structural changes in dental enamel at microscopic resolution in real-time and in a non-invasive way, with no need for extraction or processing the samples. (uniba.it)
- Multiphoton confocal microscopy provides non-invasive tissue sectioning deeper within the tissue than is available with standard conventional or visible confocal microscopic techniques so that thicker tissues can be analyzed. (utsouthwestern.edu)
- To study the clinical, histological, in vivo confocal microscopic, and molecular profile in a family with gelatinous drop-like corneal dystrophy (GDLD) from north India. (molvis.org)
- In this study, we implemented a custom-design video-rate confocal microscopy system in capable of direct visualization of fast flowing CTC at great saphenous vein (GSV) of a live animal model in vivo . (osapublishing.org)
- If CLSM data allows for in vivo use but is ultimately limited by its resolution, and SEM allows for superior resolution but cannot be performed in vivo , then modern 3D imaging techniques allow for new possibilities for in vivo medical visualization at the nanometer scale. (firstmonday.dk)
- In this study, we investigate the direct visualization of herpes simplex virus type 1 (HSV-1)-infected corneal epithelium, with in vivo confocal microscopy, using HSV-1 immunofluorescent antibodies. (umn.edu)
- Non-invasive in vivo visualization of enamel defects by reflectance confocal microscopy (RCM). (uniba.it)
Vitro and in vivo4
- There exist a number of models to study angiogenesis, both in vitro and in vivo, but there is no single perfect model so far. (diva-portal.org)
- Recent evidence suggests that distinct DC subsets direct different classes of immune responses in vitro and in vivo. (jimmunol.org)
- 9 The present study was therefore designed to elucidate and target the downstream signaling cascades in an attempt to inhibit the HGF/SF-induced angiogenesis in vitro and in vivo. (ahajournals.org)
- To refine this strategy, we have turned to malonic acid derivatives of C 60 , (C 63 ((COOH) 2 ) 3 ), synthesized and purified as two specific regioisomers with C 3 and D 3 symmetry (Fig. 1 ) and demonstrated that they are effective neuroprotective antioxidants in vitro and in vivo . (pnas.org)
- An in vivo assessment using corneal confocal bio-microscopy. (nih.gov)
- Ex vivo confocal microscopy performs real-time assessment of renal biopsy in non-neoplastic diseases. (physiciansweekly.com)
- Background Fibred confocal fluorescence microscopy (FCFM) is a novel technology that allows the in vivo assessment and quantification during bronchoscopy of the bronchial wall elastic fibre pattern, alveolar and vessel diameters and thickness of the elastic fibre in the alveolar wall. (bmj.com)
- Confocal scanning laser microscopy was an efficient and a noninvasive tool for the quantitative assessment of the conjunctival inflammation and epithelial cell densities as well as evaluation of conjunctival morphologic alterations, such as microcysts in patients with SSDE and NSSDE dry eye disease. (elsevier.com)
- Conclusions: Confocal scanning laser microscopy was an efficient and a noninvasive tool for the quantitative assessment of the conjunctival inflammation and evaluation of pathological alterations in the papillary lesions and their relation to the ocular surface disease in patients with AKC. (elsevier.com)
- Confocal microscopy is a new, emerging, noninvasive technology that can aid in the in vivo assessment of structural changes in several ocular surface diseases at the cellular level. (bioportfolio.com)
- In vivo assessment of chronological agein. (mendeley.com)
- Characteristic confocal findings for pigmented skin lesions, including BCCs and MM, have been reported. (hindawi.com)
- In this thesis, corneal lymph vessels were characterized for the first time in vivo and findings from the experimental bench could be applied in a clinical setting, where presumed lymphatics were observed in a corneal transplant patient with rejection. (diva-portal.org)
- In conclusion, we introduce a new in vivo technique for investigating angiogenesis in a corneal model were in vivo findings can be interpreted with ex vivo definitions of specific cell types by immunohistochemistry. (diva-portal.org)
- These findings were not reported previously by slit-lamp and specular microscopy. (nih.gov)
- Previous reports dealing with posterior polymorphous dystrophy (PPMD) examined using in vivo confocal microscopy reported almost the same findings, suggesting that PCV and PPMD may be the same at the microstructural level. (nih.gov)
- These findings open the possibility of assessing the in vivo effects of therapeutic interventions for COPD in future studies. (bmj.com)
- Confocal microscopy findings after endothelial transplant by DSAEK. (bioportfolio.com)
- To evaluate early in vivo corneal wound healing findings after Descemet's Stripping Automated Endothelial Keratoplasty (DSAEK) by using in vivo confocal microscopy. (bioportfolio.com)
- we compared it with in vitro findings in a rat PT cell line (WKPT) using fluorescence microscopy and flow cytometry. (pubmedcentralcanada.ca)
- The findings of the in vivo confocal microscopy were consistent with those reported in previous reports. (molvis.org)
- BACKGROUND AND OBJECTIVE: In vivo reflectance confocal microscopy (RCM) is a promising novel technology for non-invasive early diagnostics of cutaneous melanoma. (biomedsearch.com)
- The purpose of this study is to image pigmented skin lesions suspicious for melanoma with an imaging technology called in vivo reflectance confocal microscopy. (bioportfolio.com)
- The accuracy of combining the probability of BCC based on examination of clinical photographs with the predicted probability of BCC based on confocal criteria was also evaluated. (cdc.gov)
- In recent years, in vivo skin imaging devices have been successfully implemented in skin research as well as in clinical routine. (spiedigitallibrary.org)
- 2012). However, while SEM may provide superior image resolution it cannot be used in vivo , which ultimately limits its application in both clinical medicine and medical training. (firstmonday.dk)
- While the use of zStack data sets in medical imaging holds tremendous promise, one of its limitations, despite providing real time imagining of in vivo tissue, has been its application in clinical medicine. (firstmonday.dk)
- Therefore, investigating the effects of such cytokines on DC function in vivo is of paramount importance, especially from a clinical perspective. (jimmunol.org)
- However, they also provide pre-clinical ex vivo and/or intravital tools for three-dimensional quantitative assessments of cellular and molecular events, with levels of specificity and resolution difficult to achieve with other currently available modalities. (springer.com)
- These include ex vivo modalities that are currently applied primarily to pre-clinical diabetes research but have the potential to expand our scientific understanding of the disease, and may prove clinically relevant over the longer term. (springer.com)
- Reflectance confocal microscopy (RCM) in an emerging noninvasive diagnostic tool that provides in vivo tissue images at nearly cellular histological resolution. (hindawi.com)
- Ex vivo confocal microscopy is a technique for tissue examination, which generates images of fresh samples with an optical resolution comparable to those obtained by conventional pathology. (physiciansweekly.com)
- Confocal images correlated very well to the corresponding conventional histological sections, both in normal tissue and in chronic lesions (glomerulosclerosis, fibrosis and tubular atrophy). (physiciansweekly.com)
- Widely used in the biological sciences, CLSM is used to provide non-invasive optical sectioning of intact tissue in vivo, with high image resolution (Pawley 2006). (firstmonday.dk)
- The ability to obtain comprehensive structural and functional information from intact biological tissue in vivo is highly desirable for many important biomedical applications, including cancer and brain studies. (nature.com)
- Here, we developed a fully integrated multimodal microscopy that can provide photoacoustic (optical absorption), two-photon (fluorescence) and second harmonic generation (SHG) information from tissue in vivo , with intrinsically co-registered images. (nature.com)
- The reported results suggest that, by revealing complementary tissue microstructures in vivo , this multimodal microscopy can potentially facilitate a broad range of biomedical studies, such as imaging of the tumor microenvironment and neurovascular coupling. (nature.com)
- SHG microscopy is able to distinguish the microanatomical signatures of tissue under normal and malignant conditions based on the noncentrosymmetric properties of endogenously orientated biological structures, such as collagen and microtubules 13 , 14 . (nature.com)
- These instruments provide histology-quality images, without the need to remove, stain and cut the tissue, with a higher axial resolution (4 μm) than that achieved with white-light confocal microscopy (10 μm with ConfoScan 2, Nidek Technologies, Vigonza, Italy). (biomedcentral.com)
- Samples for time-lapse studies can be incubated in the Tissue Culture facility prior to imaging (to minimize transport time), which is just down the hall from the confocal microscopy room. (utsouthwestern.edu)
- Becker DE, Ancin H, Szarowski DH, Turner JN, Roysam B (1996) Automated 3-D montage synthesis from laser-scanning confocal images: application to quantitative tissue-level cytological analysis. (springer.com)
- Unlike most other vertebrate models, the zebrafish develops externally, and its transparency during embryonic and early larval stages makes it highly attractive to study tissue organogenesis using in vivo time-lapse imaging. (elifesciences.org)
- time, on excised tissue ex vivo. (dangareau.net)
- The in vivo application described is the contact-lens-induced time dependence of the fluorescence intensity from the reduced pyridine nucleotides NAD(P)H located within the mitochondria of corneal epithelial cells. (spiedigitallibrary.org)
- The confocal microscopy was used to observe the basal epithelial cell density (ECD), goblet cell density (GCD), dendritic cell density (DCD), and subepithelial collagen fiber diameter (SFD). (hindawi.com)
- In vivo confocal microscopy images showed in the superficial epithelium round and/or oval shaped zones of hyper-reflective and abnormal epithelial cells. (arvojournals.org)
- Thus in vivo confocal microscopy revealed epithelial and stromal changes compatible with an inflammatory involvement. (arvojournals.org)
- therefore, SHG microscopy enables accurate evaluation of early-stage squamous epithelial cancers. (nature.com)
- This method, based on spectral confocal reflectance microscopy (SCoRe), uses a conventional laser scanning confocal system to generate images by merging the simultaneously reflected signals from multiple lasers of different wavelengths. (leica-microsystems.com)
- In vivo confocal reflectance microscopy (CRM) is a novel technique enabling the noninvasive imaging of the skin at a cellular level resolution. (unimore.it)
- We described the characterization of three patients with TSPK by in vivo imaging with the HRTII-RCM in this present report. (molvis.org)
- Twelve Spitz and 25 melanomas (MMs) were studied by means of CRM (Vivascope 1000, Lucid Inc., USA) and digital dermoscopy (Videocap 200, DS-Mediroup, Italy) for in vivo characterization of cytological and architectural features at CRM, and correlation with dermoscopy and histology. (unimore.it)
- Howon Seo, Yoonha Hwang, Kibaek Choe, and Pilhan Kim, "In vivo quantitation of injected circulating tumor cells from great saphenous vein based on video-rate confocal microscopy," Biomed. (osapublishing.org)
Staining of the ocular surface1
- All subjects underwent corneal sensitivity measurements, the Schirmer test, tear film break-up time (BUT), fluorescein, Rose Bengal staining of the ocular surface, and confocal laser scanning microscopy of the tarsal palpebral conjunctiva. (elsevier.com)
Laser Scanning Microscopy6
- Miniprobe-based confocal laser scanning microscopy considerably increases sensitivity for the detection of biliary neoplasia and therefore represents a promising diagnostic approach. (nih.gov)
- Fluorescence confocal laser scanning microscopy for in vivo im. (mysciencework.com)
- Fluorescence confocal laser scanning microscopy for in vivo imaging of epidermal reactions to two experimental irritants. (mysciencework.com)
- Confocal microscopy , most frequently confocal laser scanning microscopy ( CLSM ), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. (wikipedia.org)
- Confocal-laser-scanning microscopy (CLSM) enables to determine in vivo histomorphological images. (egms.de)
- In vivo confocal-laser-scanning microscopy can differentiate superficial-partial vs. deep-partial thickness burns on a histomorphological level. (egms.de)
Principle of confocal1
- This diagram demonstrates the principle of confocal microscopy. (photonics.com)
- Reflectance confocal microscopy is a relatively new imaging technique that permits to evaluate in vivo skin tumors with a nearly histological resolution. (hindawi.com)
- To report a case of non-typical Pseudomonas aeruginosa keratitis that was misdiagnosed as fungal keratitis by in vivo confocal microscopy. (biomedcentral.com)
- The objective of this study was to evaluate the feasibility of using ex vivo confocal microscopy in fusion mode (reflectance and fluorescence) and the H&E-like digital staining that is obtained for the analysis of non-neoplastic kidney biopsies. (physiciansweekly.com)
- We report the results of a preliminary study of two key parameters (fluence, number of passes) vis-à-vis the feasibility of labeling and RCM imaging in human skin ex vivo , following ablation with an erbium:yttrium aluminum garnet laser. (spie.org)
- Purpose: Hyper-reflective superficial cells were an unexpected finding while examining the corneal epithelium using confocal microscopy (CM), during an MSc thesis conducted in 2006 at the University of Waterloo, Canada. (uwaterloo.ca)
- In recent years, the application of laser scanning confocal microscopy (LSCM) has provided a promising method to study the structures of the ocular surface in glaucoma [ 9 - 12 ]. (hindawi.com)
- Their work is based on the development of new in vivo confocal imaging of the ocular surface inflammation supported by Dr. Pedram Hamrah's lab at the Schepens Eye Research Institute. (contactlensupdate.com)
- The study was aimed at comparing the long-term effects of different antiglaucoma eye drops on conjunctival structures using laser scanning confocal microscopy. (hindawi.com)
- In vivo RCM images were analyzed for the arrangement of keratinocytes in epidermis, pagetoid cells and junctional melanocytic nests and correlated refractivity aspects of nests with histopathology. (biomedsearch.com)
- To present possible misdiagnosis of infectious keratitis in corneal confocal microscopy in vivo images based on typically used nomenclature. (ovid.com)
- Diagnostic process using confocal microscopy examination cannot be based just on algorithmic analysis of corneal images. (ovid.com)
- Confocal microscopy images showed hyper-reflective, thin, and branching interlocking linear structures in the stroma that were 5-8 μm in width and 200-400 μm in length. (biomedcentral.com)
- Unlike other anterior segment imaging techniques, it provides en face images of corneal cells and other structures. (utsouthwestern.edu)
- Fluorescence microscopy is unmatched in its ability to provide images with chemical specificity. (photonics.com)
- Ex vivo studies have demonstrated that topical antiglaucoma eye drops induce structural aberrations of the conjunctiva, including a decline in goblet cells, and an increase in inflammatory cells, squamous metaplasia, and subconjunctival fibrosis [ 7 , 8 ]. (hindawi.com)
- All subjects underwent the Schirmer test, tear film breakup time (BUT), vital staining, and confocal microscopy of the temporal bulbar conjunctiva. (elsevier.com)
- To analyze corneal changes in patients with superficial punctate keratitis of Thygeson using in vivo confocal microscopy. (arvojournals.org)
- Multiple articles reported that amoebic, bacterial, and fungal organisms were detected in vivo in infectious keratitis [ 1 ]. (biomedcentral.com)
- Kaufman, SCMD , Laird, JA & Beuerman, RW 1996, In-vivo immunofluorescence confocal microscopy of herpes simplex virus type 1 keratitis . (umn.edu)
Means of in vivo3
- However, the possibility to detect melanocytic atypia in nevi by means of in vivo RCM remains unknown. (biomedsearch.com)
- More accurate diagnostics by means of in vivo RCM needs further investigation on reflectance of single and nested cutaneous melanocytes in benign and malignant skin lesions. (biomedsearch.com)
- mean age: 74 ± 8.3 years which underwent DSAEK were examined by means of in vivo laser confocal microscopy between 1 and 24 months after surgery. (biomedcentral.com)
Human skin in vivo1
- Reflectance-mode confocal microscopy (RCM) offers noninvasive high-resolution imaging of human skin in vivo. (cdc.gov)
- Based on a previous study, a set of 5 histologically correlated confocal imaging criteria for diagnosing BCC was established, eg, the presence of elongated monomorphic nuclei. (cdc.gov)
- Corneal confocal microscopy could become a useful test in the study of patients with fibromyalgia. (nih.gov)
- 1 , 2 The aims of in vivo skin imaging are to study physiological as well as pathological processes and to monitor the same body site noninvasively over a specific time interval along with treatment. (spiedigitallibrary.org)
- This preliminary study demonstrated the applicability of fluorescence CLSM for a detailed study of experimental skin irritants in vivo. (mysciencework.com)
- However the study of DCs in vivo has been difficult because of their rarity in the blood and in other tissues. (jimmunol.org)
- This study identifies ADAM10 as the in vivo CD23 sheddase and an important regulator of B cell development. (rupress.org)
- Such a new technology would benefit all areas of cellular biology, but it would be particularly useful for the study of T cells under physiological conditions in vivo, where both the time and frequency of signaling are critical to their differentiation. (rupress.org)
- The transparency and rapid embryogenesis of zebrafish, together with fluorescent reporters, allow for the study of developmental processes with single-cell resolution using in vivo time-lapse imaging ( Beis and Stainier, 2006 ). (elifesciences.org)
- Human islets transduced with cdk-6 and cyclin D 1 were transplanted into diabetic NOD-SCID mice and markedly outperformed native human islets in vivo, maintaining glucose control for the entire 6 weeks of the study. (diabetesjournals.org)
- As a result, multiphoton microscopy enables longitudinal monitoring of physiological processes in small animals in vivo . (nature.com)
- CONCLUSIONS: Nevus with histopathologically confirmed nevomelanocytic atypia (dysplastic nevus) could not be distinguished from nevus without atypia using analyzed in vivo RCM features of melanocytic atypia. (biomedsearch.com)
- In vivo examination of the skin by reflectance confocal microscopy (RCM) has been performed for about 20 years, leading to a broad spectrum of imaged infectious and inflammatory skin diseases (ISD) with many described RCM features. (ru.nl)
- The confocal scope has become pivotal to several projects because it permits sequential examination of intraocular structures over an extended period. (evms.edu)
- We report a new technique for high-resolution in vivo imaging of myelinated axons in the brain, spinal cord and peripheral nerve that requires no fluorescent labeling. (leica-microsystems.com)
- Tocky uses the fluorescent Timer protein, which spontaneously shifts its emission spectrum from blue to red, in combination with computer algorithms to reveal the dynamics of differentiation in vivo. (rupress.org)
- Confocal microscopes work on the principle of point excitation in the specimen (diffraction limited spot) and point detection of the resulting fluorescent signal. (wikipedia.org)
- To overcome this limitation, a new imaging modality was evaluated to detect neoplasia in vivo in the biliary tract. (nih.gov)
- Mucosal imaging was performed with a miniaturized confocal laser scanning miniprobe introduced via the accessory channel of a cholangioscope. (nih.gov)
- The fluorophore indocyanine green (ICG) was intradermally injected into healthy forearm skin of 10 volunteers followed by in vivo imaging at various time points. (spiedigitallibrary.org)
- Also of critical importance, is that these medical imaging technologies can be performed in real time on in vivo specimens (University of Houston 2014). (firstmonday.dk)
- Using SCoRe we performed chronic brain imaging up to 400 μm deep, capturing for the first time de novo myelination of mouse cortical axons in vivo. (leica-microsystems.com)
- at the other end, genuine microscopy (confocal) has the highest imaging resolution but the lowest depth penetration. (springer.com)
- To investigate the synaptic basis of this dysfunction, we employed in vivo two-photon imaging to analyse excitatory postsynaptic dendritic protrusions. (nature.com)
- Mounting leaves of Arabidopsis thaliana in PFD significantly improved the optical qualities of the leaf, thereby enabling high-resolution laser scanning confocal imaging over twofold deeper into the mesophyll, compared with using water. (plymouth.ac.uk)
- We used two-photon microscopy imaging of GFP-based probes expressed in mouse skeletal muscles to monitor, for the first time in a live animal, the dynamics of [Ca 2+ ] SR and cAMP. (rupress.org)
- Since multiphoton confocal microscopy uses infrared light for excitation, there is less cellular phototoxicity associated with imaging of live cells. (utsouthwestern.edu)
- Bussau LJ, Vo LT, Delaney PM, Papworth GD, Barkla DH, King RG (1998) Fibre optic confocal imaging (FOCI) of keratinocytes, blood vessels and nerves in hairless mouse skin in vivo. (springer.com)
- Delaney PM, King RG, Lambert JR, Harris MR (1994) Fibre optic confocal imaging (FOCI) for subsurface microscopy of the colon in vivo. (springer.com)
- Eschbacher J, Martirosyan NL, Nakaji P, Sanai N, Preul MC, Smith KA et al (2012) In vivo intraoperative confocal microscopy for real-time histopathological imaging of brain tumors. (springer.com)
- Here, using in vivo time-lapse imaging and genetic analyses in zebrafish, we determined the events leading to islet innervation. (elifesciences.org)
- Wide-field in vivo oral OCT imaging. (sfu.ca)
- Reflectance confocal microscopy (RCM) is a noninvasive imaging tool that allows for in vivo imaging of the skin at quasihistological resolution. (mendeley.com)
- Confocal live imaging of Candida albicans in mouse ear ( in vivo ). (rochester.edu)
- Several imaging and laser industry experts spoke with BioPhotonics , offering their insights into the advancement of confocal microscopy technology, novel improvements for more effective and efficient application in the life sciences, and what it all means for the future. (photonics.com)
- A - F ) Schematic ( A ) and confocal ( B - F ) in vivo time-lapse imaging of angioblasts (green) and their migration in a Tg(fli1a:EGFP) y1 embryo, injected with H2B-mCherry mRNA (purple, all nuclei), dorsal views at indicated time points. (elifesciences.org)
- Leaves were mounted in the nontoxic, nonfluorescent perfluorocarbon, perfluorodecalin (PFD), and optical enhancement and physiological effect were assessed using confocal microscopy and chlorophyll fluorescence. (plymouth.ac.uk)
- We conclude that the application of PFD as a mounting medium substantially increases confocal image resolution of living mesophyll and vascular bundle cells, with minimal physiological impact. (plymouth.ac.uk)
- The possibility to generate oblique sections using isotropic volume stacks opens the window to slit lamp microscopy on a cellular level. (kit.edu)
- Intraoperative confocal microscopy is an emerging and practicable technology for the resection of human brain tumors. (springer.com)
- Insulin uptake in rabbit PT has also been monitored by electron microscopy (EM) after perfusion of gold-labeled insulin and subsequent cross-linking to its receptor with disuccinimidylsuberate ( 18 ). (pubmedcentralcanada.ca)
- Observations by electron microscopy revealed the presence at the surfaces of the mutant bacteria of fibrillar structures looking like the particular fimbriae described by the Olsén group and designated curli (A. Olsén, A. Jonsson, and S. Normark, Nature 338:652-655, 1989). (asm.org)
- However, confocal microscopy has shown that UV irradiation or treatment with certain DNA-damaging agents induces the translocation of pol η to stalled DNA replication forks, which is seen as formation of foci in the nucleus ( 13 ). (pnas.org)
- We have shown that confocal microscopy detects more subtle signs of inflammation than by slit lamp alone. (evms.edu)
- Perfluorodecalin enhances in vivo confocal microscopy resolution of Arabidopsis thaliana mesophyll. (plymouth.ac.uk)
- In vivo RCM feature of dense cell clusters corresponded with melanin containing nevomelanocytes on histopathology though exact correspondence of non-homogeneous and atypical sparse cell clusters remained questionable. (biomedsearch.com)
- To describe and evaluate the utility of reflectance confocal microscopy (RCM) in PLM and to establish a correlation between dermoscopy, RCM, histopathology and immunohistochemistry. (physiciansweekly.com)
- OBJECTIVES To comprehensively inventory the proteins that control the G1/S cell cycle checkpoint in the human islet and compare them with those in the murine islet, to determine whether these might therapeutically enhance human β-cell replication, to determine whether human β-cell replication can be demonstrated in an in vivo model, and to enhance human β-cell function in vivo. (diabetesjournals.org)