Glucans: Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.beta-Glucans: Glucose polymers consisting of a backbone of beta(1->3)-linked beta-D-glucopyranosyl units with beta(1->6) linked side chains of various lengths. They are a major component of the CELL WALL of organisms and of soluble DIETARY FIBER.DextranaseGlucosyltransferases: Enzymes that catalyze the transfer of glucose from a nucleoside diphosphate glucose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.Streptococcus mutans: A polysaccharide-producing species of STREPTOCOCCUS isolated from human dental plaque.Dental Pellicle: A thin protein film on the surface of DENTAL ENAMEL. It is widely believed to result from the selective adsorption of precursor proteins present in SALIVA onto tooth surfaces, and to reduce microbial adherence to the TEETH.Glucan Endo-1,3-beta-D-Glucosidase: An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman.PolysaccharidesAmylopectin: A highly branched glucan in starch.Glycogen Debranching Enzyme System: 1,4-alpha-D-Glucan-1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase/dextrin 6 alpha-D-glucanohydrolase. An enzyme system having both 4-alpha-glucanotransferase (EC 184.108.40.206) and amylo-1,6-glucosidase (EC 220.127.116.11) activities. As a transferase it transfers a segment of a 1,4-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or another 1,4-alpha-D-glucan. As a glucosidase it catalyzes the endohydrolysis of 1,6-alpha-D-glucoside linkages at points of branching in chains of 1,4-linked alpha-D-glucose residues. Amylo-1,6-glucosidase activity is deficient in glycogen storage disease type III.Isoamylase: An enzyme that hydrolyzes 1,6-alpha-glucosidic branch linkages in glycogen, amylopectin, and their beta-limit dextrins. It is distinguished from pullulanase (EC 18.104.22.168) by its inability to attack pullulan and by the feeble action of alpha-limit dextrins. It is distinguished from amylopectin 6-glucanohydrolase (EC 22.214.171.124) by its action on glycogen. With EC 126.96.36.199, it produces the activity called "debranching enzyme". EC 188.8.131.52.Glucan 1,3-beta-Glucosidase: An exocellulase with specificity for 1,3-beta-D-glucasidic linkages. It catalyzes hydrolysis of beta-D-glucose units from the non-reducing ends of 1,3-beta-D-glucans, releasing GLUCOSE.Amylose: An unbranched glucan in starch.alpha-Cyclodextrins: Cyclic GLUCANS consisting of six (6) glucopyranose units linked by 1,4-glycosidic bonds.Chicory: A thick-rooted perennial (Cichorium intybus) native to Europe but widely grown for its young leaves used as salad greens and for its roots, dried and ground-roasted, used to flavor or adulterate coffee. (From Webster, 3d ed)Glycoside HydrolasesPolysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Lentinan: Polysaccharide isolated from the edible mushroom LENTINULA EDODES. The exact composition is unknown.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Periplasm: The space between the inner and outer membranes of a cell that is shared with the cell wall.Carbohydrate Conformation: The characteristic 3-dimensional shape of a carbohydrate.Streptococcus: A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.Dextrans: A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.Isomaltose: A disaccharide consisting of two glucose units in an alpha (1-6) glycosidic linkage.Medicine, East Asian Traditional: Medical practice or discipline that is based on the knowledge, cultures, and beliefs of the people in EAST ASIA.Streptococcus sobrinus: A species of gram-positive, coccoid bacteria isolated from the human tooth surface. Strains have been shown to be cariogenic in experimental animals and may be associated with human dental caries.Starch: Any of a group of polysaccharides of the general formula (C6-H10-O5)n, composed of a long-chain polymer of glucose in the form of amylose and amylopectin. It is the chief storage form of energy reserve (carbohydrates) in plants.DextrinsSpectrometry, Mass, Fast Atom Bombardment: A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.Sucrose: A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.Sizofiran: A beta-D-glucan obtained from the Aphyllophoral fungus Schizophyllum commune. It is used as an immunoadjuvant in the treatment of neoplasms, especially tumors found in the stomach.Chitin: A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.Durapatite: The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.Paecilomyces: A mitosporic fungal genus occasionally causing human diseases such as pulmonary infections, mycotic keratitis, endocarditis, and opportunistic infections. Its teleomorph is BYSSOCHLAMYS.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Uridine Diphosphate Glucose: A key intermediate in carbohydrate metabolism. Serves as a precursor of glycogen, can be metabolized into UDPgalactose and UDPglucuronic acid which can then be incorporated into polysaccharides as galactose and glucuronic acid. Also serves as a precursor of sucrose lipopolysaccharides, and glycosphingolipids.Rhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Shiitake Mushrooms: Mushrooms in the order AGARICALES containing B vitamins, cortinelin, and the polysaccharide LENTINAN.gamma-Cyclodextrins: Cyclic GLUCANS consisting of eight (8) glucopyranose units linked by 1,4-glycosidic bonds.Oligosaccharides: Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.Air Microbiology: The presence of bacteria, viruses, and fungi in the air. This term is not restricted to pathogenic organisms.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Cyclodextrins: A homologous group of cyclic GLUCANS consisting of alpha-1,4 bound glucose units obtained by the action of cyclodextrin glucanotransferase on starch or similar substrates. The enzyme is produced by certain species of Bacillus. Cyclodextrins form inclusion complexes with a wide variety of substances.Mitosporic Fungi: A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.Cellulases: A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.Sodium Hydroxide: A highly caustic substance that is used to neutralize acids and make sodium salts. (From Merck Index, 11th ed)Streptococcus sanguis: A gram-positive organism found in dental plaque, in blood, on heart valves in subacute endocarditis, and infrequently in saliva and throat specimens. L-forms are associated with recurrent aphthous stomatitis.Rhizobiaceae: A family of gram-negative bacteria which are saprophytes, symbionts, or plant pathogens.Dental Plaque: A film that attaches to teeth, often causing DENTAL CARIES and GINGIVITIS. It is composed of MUCINS, secreted from salivary glands, and microorganisms.Mannans: Polysaccharides consisting of mannose units.Candida albicans: A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).Lectins, C-Type: A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.Agglutination: The clumping together of suspended material resulting from the action of AGGLUTININS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.Apoenzymes: The protein components of enzyme complexes (HOLOENZYMES). An apoenzyme is the holoenzyme minus any cofactors (ENZYME COFACTORS) or prosthetic groups required for the enzymatic function.
... is an antifungal pyridobenzimidazole derivative that inhibits β-glucan synthesis. D11-2040 and D21-6076 are antifungal ... Kitamura, Akihiro; Someya, Kazuhiko; Okumura, Ryo; Hata, Masato; Takeshita, Hiroshi; Nakajima, Ryohei (2010). "In Vitro ... 6-Glucan Synthesis". Antimicrobial Agents and Chemotherapy. 53 (2): 670-7. doi:10.1128/AAC.00844-08. PMC 2630612 . PMID ... Antifungal Activities of D11-2040, a β-1,6-Glucan Inhibitor, with or without Currently Available Antifungal Drugs". Biological ...
An in vitro experiment showed lentinan stimulated production of white blood cells in the human cell line U937. Lentinan is ... Lentinan is a β-1,3 beta-glucan with β-1,6 branching. It has a molecular weight of 500,000 Da and specific rotation of +14-22 ... Lentinex is a beta-glucan formulation containing lentinan which is marketed as a novel food in the EU. Medicinal mushrooms ... July 2009). "Efficacy of Orally Administered Superfine Dispersed Lentinan (β-1,3-Glucan) for the Treatment of Advanced ...
A polysaccharide called β-D-Glucan from P. pulmonarius reduces sensitivity to pain in mice, and could be an "attractive" basis ... Several studies done on animals and in vitro suggest P. pulmonarius and its extracts may have possible medicinal applications ... In a different study on mice, a glucan from P. pulmonarius showed potent anti-inflammatory and analgesic properties. A methanol ... Extracts of P. pulmonarius have antimicrobial properties and exhibit antioxidant activity in vitro. List of Pleurotus species ...
The cell walls of the hyphae are variably composed of chitin and β-glucans, just as in Basidiomycota. However, these fibers are ... Yeasts for small colonies in vitro and in vivo, and excessive growth of Candida species in the mouth or vagina causes "thrush ... The cell walls of the ascomycetes almost always contain chitin and β-glucans, and divisions within the hyphae, called "septa", ...
One example is the CBM11 of Clostridium thermocellum Cel26A-Cel5E, this domain has been shown to bind both β-1,4-glucan and β-1 ... Carbohydrate-binding module family 49 (CBM49) is found at the C-terminal of cellulases and in vitro binding studies have shown ... Isoamylase hydrolyses 1,6-alpha-D-glucosidic branch linkages in glycogen, amylopectin and dextrin; 1,4-alpha-glucan branching ... 3-mixed linked glucans only bind in cleft B. Carbohydrate-binding module family 9 (CBM9) binds to crystalline cellulose. CBM4 ...
In vitro and mouse models show echinocandins may also enhance host immune responses by exposing highly antigenic beta-glucan ... 3-D-glucan synthase enzyme complex in susceptible fungi to disturb fungal cell glucan synthesis. Beta-glucan destruction ... Beta glucans are carbohydrate polymers that are cross-linked with other fungal cell wall components (The bacterial equivalent ... Echinocandins are a new class of antifungal drugs that inhibit the synthesis of glucan in the cell wall, via noncompetitive ...
Cellulose synthase (GDP-forming)
Chambers J, Elbein AD (1970). "Biosynthesis of glucans in mung bean seedlings. Formation of beta-(1,4)-glucans from GDP-glucose ... Flowers HM, Batra KK, Kemp J, Hassid WZ (1969). "Biosynthesis of cellulose in vitro from guanosine diphosphate D-glucose with ... The systematic name of this enzyme class is GDP-glucose:1,4-beta-D-glucan 4-beta-D-glucosyltransferase. Other names in common ... 4-beta-glucan glucosyltransferase, and guanosine diphosphoglucose-cellulose glucosyltransferase. This enzyme participates in ...
Mannan oligosaccharide-based nutritional supplements
Glycosidic bond Mannan Mannose Saccharomyces cerevisiae extracts PGG-glucan, EpiCor, nutritional yeast Oyofo, BA; Deloach, JR; ... "Inhibition by mannose of in vitro colonization of chicken small intestine by Salmonella typhimurium". Poultry science. 68 (10 ... Kath, Franziskus; Kulicke, Werner-Michael (1999). "Mild enzymatic isolation of mannan and glucan from yeastSaccharomyces ...
6-glucan (including amylopectin) Ai Y, Nelson B, Birt DF, Jane JL (2013). "In vitro and in vivo digestion of octenyl succinic ... dextran, α-1,6-glucan glycogen, α-1,4- and α-1,6-glucan pullulan, α-1,4- and α-1,6-glucan starch, α-1,4- (such as amylose) and ... α-Glucans (alpha-glucans) are polysaccharides of D-glucose monomers linked with glycosidic bonds of the alpha form. ... 1] Page that explains alpha-glucan linkages in starch.. ...
"Effects of high-resistant-starch banana flour (RS2) on in vitro fermentation and small-bowel excretion of energy, nutrients, ... "Effects of banana flour and β-glucan on the nutritional and sensory evaluation of noodles." Food Chemistry 119.1 (2010): 34-40 ... "Effects of high-resistant-starch banana flour (RS2) on in vitro fermentation and small-bowel excretion of energy, nutrients, ... "Resistant starch contents and the in vitro starch digestibility of Thai starchy foods." Kasetsart Journal (Natural Sciences) 43 ...
Choromanska, Anna (16 June 2015). "Anticancer properties of low molecular weight oat beta-glucan - An in vitro study". ... Oat β-glucans are linear and linked at the 1,3 and 1,4 carbon sites. Oat β-glucans can form into a random coil structure and ... In oats, β-glucan makes up the majority of the soluble fibre; however, oat β-glucans do become insoluble above a certain ... Oat β-glucans are water-soluble β-glucans derived from the endosperm of oat kernels known for their cholesterol lowering and ...
cytosine phosphate-guanosine, oligodeoxynucleotides, glucans Activation immunotherapies. Cancer. Main article: ... The extraction of G-CSF lymphocytes from the blood and expanding in vitro against a tumour antigen before reinjecting the cells ... Adoptive cell transfer in vitro cultivates autologous, extracted T cells for later transfusion. ... cytotoxic T lymphocytes and other relevant immune cells are expanded in vitro and then reinfused. The therapy has been ...
Luis Federico Leloir
The extraction of G-CSF lymphocytes from the blood and expanding in vitro against a tumour antigen before reinjecting the cells ... oligodeoxynucleotides and glucans are involved in clinical and preclinical studies. Immunomodulators are the active agents of ... Adoptive cell transfer in vitro cultivates autologous, extracted T cells for later transfusion. The T cells may already target ... cytotoxic T lymphocytes and other relevant immune cells are expanded in vitro and then reinfused. The therapy has been tested ...
Experiments in the 1980s concluded that two glucans isolated from the species showed potent antitumour properties when used on ... Another study reported that the species may be effective in stopping platelet binding in vitro, with possible uses regarding ... β-D-glucan of Auricularia auricula-judae, and other polysaccharides containing (1→3)-glycosidic linkages". Carbohydrate ...
β-1,3-glucan binding protein present in C. cupreum bind specifically to β-1,3-glucan and lipoteichoic acids in the cell wall of ... Pigment produced by C. cupreum has in vitro antagonistic activity against the phytopathogenic bacterium, Ralstonia solanacearum ... The cell wall of C. cupreum is largely composed of chitin and glucan, which is reflected in the large number of acquired genes ... 3-glucans. These and other hydrolases targeting fungal cell wall components function synergistically and are presumed to play ...
Oral mucosa tissue engineering
Glucan is a polysaccharide with antibacterial, antiviral and anticoagulant properties. Hyaluronic acid is added to improve the ... Examples of hybrid scaffolds are HYAFF® and Laserskin®. These hybrid scaffolds have been shown to have good in-vitro and in- ... and submicron-scale fibrous scaffolds from polymer solutions specifically intended for use as in vitro cell and tissue ... Gelatin-oxidized dextran matrix Gelatin-chitosan-oxidized dextran matrix Gelatin-glucan matrix Gelatin-hyaluronate matrix ...
... inhibits glucan synthase, an enzyme important in the formation of (1→3)-β-D-glucan, a major fungal cell wall ... Pfaller MA, Boyken L, Hollis RJ, Messer SA, Tendolkar S, Diekema DJ (2005). "In Vitro Activities of Anidulafungin against More ... Glucan synthase is not present in mammalian cells, so it is an attractive target for antifungal activity. Anidulafungin is ... β-D-glucan synthase, an enzyme important to the synthesis of the fungal cell wall. Candidemia and other forms of invasive ...
In in vitro studies, the fungus has been shown to grow when inoculated into soil and sterile horse or cow excrement. The ... The yeast form contains large amounts of α-(1,3)-linked glucan. The chitin content of the mycelial form is greater than that of ... The mycelial form of P. brasiliensis can be converted to the yeast form in vitro by growth on brain heart infusion agar or ... The hormone estrogen is thought to inhibit the transformation of the mycelial to the yeast form, as supported by in vitro ...
These compounds are being studied in vivo and in vitro for possible effects on the cardiovascular system, immune system and ... "Influence of Cranberry Juice on Glucan-Mediated Processes Involved in Streptococcus mutans Biofilm Development". Caries ... Phenolic Characterization and in Vitro Bioactivity". Journal of Agricultural and Food Chemistry. 60 (13): 3396-408. doi:10.1021 ...
β-D-glucan assays were suggested as a potential method for early detection. The β-D-glucan assay detects (1,3)-β-D-glucan, a ... In vitro studies of E. rostratum growth show sporulation to be completely inhibited by light at 34 °C (93 °F) but not at lower ... Revankar, S. G.; Moudgal, V.; Chandrasekar, P.; Sobel, J. D. (24 March 2014). "In Vitro Studies of Exserohilum rostratum with ... Optimum sporulation in continuous light occurs 28-31 °C (82-88 °F) in vitro. At these lower temperatures, sporulation shifts ...
... inhibits glucan synthase, an enzyme important in the formation of (1→3)-β-D-glucan, a major fungal cell wall ... "In Vitro Activities of Anidulafungin against More than 2,500 Clinical Isolates of Candida spp., Including 315 Isolates ... Glucan synthase is not present in mammalian cells, so it is an attractive target for antifungal activity. ... D-glucan synthase, an enzyme important to the synthesis of the fungal cell wall. ...
Many fungal isolates act as DPP-4 inhibitors, alpha-glucosidase inhibitors, and alpha amylase inhibitors in vitro. Ternatin is ... Some countries have approved Beta-glucan fungal extracts lentinan, polysaccharide-K, and polysaccharide peptide as immunologic ... Vegemite Marmite Cenovis Promite Guinness Yeast Extract PGG-glucan MOS Nutritional yeast Yeast extract Nicotinamide riboside ...
Chua SL, Hultqvist LD, Yuan M, Rybtke M, Nielsen TE, Givskov M, Tolker-Nielsen T, Yang L (August 2015). "In vitro and in vivo ... which encodes periplasmic glucans that may interact with antibiotics and cause them to become sequestered into the periplasm. ... When grown in vitro conditions designed to mimic a cystic fibrosis patient's lungs, these genes mutate repeatedly. Probiotic ... P. aeruginosa is often preliminarily identified by its pearlescent appearance and grape-like or tortilla-like odor in vitro. ...
Imunostimulacija - Wikipedija
Adult polyglucosan body disease
In addition to beta glucans, the mushroom's effect on the immune system is believed to be due to other polysaccharides, such as ... Watanabe, T; Nakajima, Y; Konishi, T (2008). "In vitro and in vivo anti-oxidant activity of hot water extract of basidiomycetes ... Because Agaricus subrufescens contains a high level of beta glucans, compounds known for stimulating the immune system, the ... doi:10.1111/j.1365-2621.1984.tb10433.x. Hetland, Geir; Sandven, P. (2002). "beta-1,3-Glucan reduces growth of Mycobacterium ...
In an in vitro environment, terbinafine has been found to work in synergy with azoles against P. boydii. Echinocandins, such as ... Immune response is characterized by TLR2 recognition of P. boydii derived α-glucans, while TLR4 mediates the recognition of P. ... CMT-3, a chemically modified tetracycline, has also shown to be active in vitro against P. boydii. In the United States, P. ... caspofungin and sordarins, have shown promise in in vitro assays. ...
ATP-binding cassette transporter
Family 3.A.1.108 The β-Glucan Exporter (GlucanE) Family 3.A.1.109 The Protein-1 Exporter (Prot1E) Family 3.A.1.110 The Protein- ... "P-glycoprotein-mediated in vitro biliary excretion in sandwich-cultured rat hepatocytes". Drug Metab Dispos. 29 (10): 1277-83. ... family 3.A.1.108 β-Glucan exporter (GlucanE) family 3.A.1.109 Protein-1 exporter (Prot1E) family 3.A.1.110 Protein-2 exporter ( ...
Experiments in the 1980s concluded that two glucans isolated from the species showed potent antitumour properties when used on ... Another study reported that the species may be effective in stopping platelet binding in vitro, with possible uses ... β-D-glucan of Auricularia auricula-judae, and other polysaccharides containing (1→3)-glycosidic linkages". Carbohydrate ...
Son HJ, Bae HC, Kim HJ, Lee DH, Han D, Park J (2005). "Effects of β-glucan on proliferation and migration of fibroblasts". ... Newton PM, Watson JA, Wolowacz RG, Wood EJ (August 2004). "Macrophages restrain contraction of an in vitro wound healing model ... which can be modelled in vitro using the collagen gel contraction assay or the dermal equivalent model. ... "Human keratocyte migration into collagen gels declines with in vitro ageing". Mechanisms of Ageing and Development. 119 (3): ...
Beta-glucan from oat bran, whole oats, oatrim, or rolled oats (3 grams per day) ... an in vitro study". British Journal of Nutrition. 74 (2): 221-27. doi:10.1079/BJN19950125.. ... 3 g or more per day of beta-glucan soluble fiber from either whole oats or barley, or a combination of whole oats and barley ... Some viscous fibers may also be partially or fully fermented within the intestinal tract (guar gum, beta-glucan, glucomannan ...
Naturally occurring phenols
Production of phenolic compounds by Spirulina maxima microalgae and their protective effects in vitro toward hepatotoxicity ... In vitro measurements. Other tests measure the antioxidant capacity of a fraction. Some make use of the 2,2'-azino-bis(3- ... vanillin have been isolated from in vitro culture of the freshwater green alga Spongiochloris spongiosa. ... assays or inhibition of copper-catalyzed in vitro human low-density lipoprotein oxidation. ...
پلیفنل - ویکیپدیا، دانشنامهٔ آزاد
Compared with the effects of polyphenols in vitro, the possible functions in vivo remain unknown due to 1) the absence of ... Complex gallotannins develop through the in-vitro oxidation of 1,2,3,4,6-pentagalloyl-glucose or dimerization processes ... assays or inhibition of copper-catalyzed in vitro human low-density lipoprotein oxidation. ... unphysiological test concentrations in the in vitro studies, which are subsequently irrelevant for the design of in vivo ...
The cell walls of the hyphae are variably composed of chitin and β-glucans, just as in Basidiomycota. However, these fibers are ... Yeasts for small colonies in vitro and in vivo, and excessive growth of Candida species in the mouth or vagina causes "thrush ... The cell walls of the ascomycetes almost always contain chitin and β-glucans, and divisions within the hyphae, called "septa", ...
Tamás L, Huttová J, Mistrk I, Kogan G (2002). "Eﬀect of Carboxymethyl Chitin-Glucan on the Activity of Some Hydrolytic Enzymes ... Toxicology in Vitro. 27 (5): 1489-1495. doi:10.1016/j.tiv.2013.03.002. PMID 23524101. Chou, Janice Y.; Robinson, J. C. (1977-01 ... "Induction of Placental Alkaline Phosphatase in Choriocarcinoma Cells by 5-Bromo-2'-Deoxyuridine". In Vitro. 13 (7): 450-460. ...
Viscous fiber sources gaining FDA approval are: Psyllium seed husk (7 grams per day) Beta-glucan from oat bran, whole oats, ... an in vitro study". British Journal of Nutrition. 74 (2): 221-27. doi:10.1079/BJN19950125. Boerjan, Wout; Ralph, John; Baucher ... Eligible sources of soluble fiber providing beta-glucan include: Oat bran Rolled oats Whole oat flour Oatrim Whole grain barley ... Some viscous fibers may also be partially or fully fermented within the intestinal tract (guar gum, beta-glucan, glucomannan ...
Dental caries is caused by the formation of insoluble glucan (plaque) on the surface of teeth, and the production of acids by ... A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides. J Appl Microbiol 91(5):878-887 ... 1989). Caries-inducing activity of isomaltooligosugar (IMOS) in vitro and rat experiments. Shoni Shikagaku Zasshi 27(4) 1010-7 ... 1989). Caries-inducing activity of isomaltooligosugar (IMOS) in vitro and rat experiments. Shoni Shikagaku Zasshi 27(4) 1010-7 ...
Planetary Herbals - Planetary Herbals - Shiitake Mushroom Supreme 650 mg - 50 tabs
Reishi has been shown to support immune resistance in modern animal and in vitro studies. Shiitake Mushroom Supreme combines a ... Beneficial Constituents Shiitake is a rich source of lentinan, a highly purified glucan extracted from the mycelium and the ... Beneficial Constituents Shiitake is a rich source of lentinan, a highly purified glucan extracted from the mycelium and the ... Reishi has been shown to support immune resistance in modern animal and in vitro studies. Shiitake Mushroom Supreme combines a ...
Influence of Yeast β-Glucan on Cookies Sensory Characteristics and Bioactivities
... glucan as low-cost food ingredient in formulating cookies at 2% offers exciting new use of ,i,β,/i,-glucan of yeast origin. ... glucan in deterring oxidative. It is further noted that 2 or 4% ,i,β,/i,-glucan incorporated cookies assimilated highest ... The aim of this study was to determine the effect of substituting wheat flour by 1, 2, and 4% yeast isolated ,i,β,/i,-glucan in ... i,β,/i,-Glucan fortification in foods is technologically and economically feasible, suggesting that a significant prospect of , ...
Synergism between cucumber alpha-expansin, fungal endoglucanase and pectin lyase. | Sigma-Aldrich
Both Cel12A and Cel5A were able to hydrolyze cellulose CMC-Na and mixed-linkage beta-glucan. In contrast to Cel5A, Cel12A could ... also hydrolyze xyloglucan and induce wall extension of cucumber hypocotyls in an in vitro assay. Combining alpha-expansin, even ... alpha-Expansin enhanced Cel12As hydrolytic activity on cellulose CMC-Na but not on xyloglucan and beta-glucan. Expansin did ...
The effects of -glucan on human immune and cancer cells. Journal of Hematology & Oncology 2009, 2:25 (10 June 2009). 2009. * ... In vitro antioxidant activity and hepatoprotective effects of Lentinula edodes against paracetamol-induced hepatotoxicity. ... Rop O, Mlcek J, and Jurikova T. Beta-glucans in higher fungi and their health effects. Nutr Rev. 2009 Nov;67(11):624-31. Review ... Eritadenine from Edible Mushrooms Inhibits Activity of Angiotensin Converting Enzyme in Vitro. J Agric Food Chem. 2016 Mar 23; ...
A Second-Generation Fungerp Analog, SCY-247, Shows Potent In Vitro Activity against Candida auris and Other Clinically Relevant...
... d-glucan. We conducted an extensive antifungal screen of SCY-247 against yeast and mold strains compared with the parent ... A Second-Generation Fungerp Analog, SCY-247, Shows Potent In Vitro Activity against Candida auris and Other Clinically Relevant ... A Second-Generation Fungerp Analog, SCY-247, Shows Potent In Vitro Activity against Candida auris and Other Clinically Relevant ... A Second-Generation Fungerp Analog, SCY-247, Shows Potent In Vitro Activity against Candida auris and Other Clinically Relevant ...
Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci |...
Extracellular glucans synthesized by strains of two types of Streptococcus mutans in vitro. ... Mechanism of glucan-induced agglutination in Streptococcus mutans. I. Binding of radioactive glucan to whole cells of S. mutans ... Inhibitory effects of endo-alpha-1,3-glucanase on glucan film formation and glucan synthesis by the glucosyltransferase of the ... Effects of exogenous insoluble glucan primer on insoluble glucan synthesis by Streptococcus mutans. ...
The structure of water-insoluble glucans of cariogenic Streptococcus mutans, formed in the absence and presence of dextranase |...
Extracellular polysaccharide synthesized by the oral bacterium Streptococcus mutans of serotype a to e in vitro. ... Mechanism of glucan-induced agglutination in Streptococcus mutans. I. Binding of radioactive glucan to whole cells of S. mutans ... Molecular modeling study of highly branching (1--,3)-alpha-D-glucan, a model polysaccharide for cariogenic glucan, using the N- ... Inhibitory effects of endo-alpha-1,3-glucanase on glucan film formation and glucan synthesis by the glucosyltransferase of the ...
Current Drug Delivery | Bentham Science
Physiochemical Characterization of β-glucan and in vitro Release of Lactoferrin from β-glucan Microparticles ... In Vitro Drug Release Behavior, Mechanism and Antimicrobial Activity of Rifampicin Loaded Low Molecular Weight PLGA-PEG-PLGA ... Development and in vitro Evaluation of Diclofenac Sodium Loaded Mucoadhesive Microsphere with Natural Gum for Sustained ... Solid Lipid Nanoparticles (SLNs) Gels for Topical Delivery of Aceclofenac in vitro and in vivo Evaluation ...
... glucan-stimulated human macrophages differentiated in vitro from primary blood monocytes. We show that ?-glucan stimulation ... glucan-stimulated human macrophages differentiated in vitro from primary blood monocytes. We show that ?-glucan stimulation ... Fungi express a variety of pathogen-associated molecular patterns on their surface including ?-glucans, which are important ... Fungi express a variety of pathogen-associated molecular patterns on their surface including ?-glucans, which are important ...
The dual effect of curcumin nanoparticles encapsulated by 1-3/1-6 β-glucan from medicinal mushrooms Hericium erinaceus and...
The dual effect of curcumin nanoparticles encapsulated by 1-3/1-6 β-glucan from medicinal mushrooms Hericium erinaceus and ... higher water solubility and better in vitro anti-cancer effects compared to free curcumin and 1-3/1-6 β-glucan, expectedly. ... Therefore, the objective of this project is to prepare nanoparticles by polymeric encapsulating curcumin by 1-3/1-6 β-glucan ... 0 Journal Article %T The dual effect of curcumin nanoparticles encapsulated by 1-3/1-6 β-glucan from medicinal mushrooms ...
Research Outputs - CUHK Research Portal - CONVERIS Research Information System
Publications | Galan Research Group
Journal of Cereal Science
Harry Wichers - WUR
A variety of in vitro and ex vivo read-out systems is available for this purpose. Studying and characterisation of food ... The focus is in particular aimed at the immunomodulatory properties of β-glucans (cell wall fragments from micro organisms) and ... in in vitro mimicked inflammation processes or via their effect on the activity of cultured cells that are involved in ...
FBG - Allie: Result by abbreviation
Temat: 'Gut microbiota' - OpacWWW - Prolib Integro
ASMscience | Role of Antibody-Mediate
... in vitro studies, and human studies. Monoclonal antibody (MAb) technology, described for the first time in the 1970s, allowed ... Glucan is a component of the Mycobacterium tuberculosis surface that is expressed in vitro and in vivo. Infect. Immun. 70: 2566 ... Expression of a Mycobacterium tuberculosis arabinomannan antigen in vitro and in vivo. Infect. Immun. 69: 5671- 5678.. ... The destruction of tubercle bacilli within phagocytes in vitro. J. Infect. Dis. 58: 64- 69.. ...
Barley Grass Inhibits 73% of Leukemia Cells in Vitro « New Drug Approvals
... in vitro, and killed 62% of the cancer cells outright via apoptosis and necrosis. The barley grass extract also potently ... of Leukemia Cells in Vitro: An extract of green barley grass (Hordeum vlgare L.) powder was shown to inhibit the proliferation ... immune-stimulating glucans, and ribosome inactivating protein conjugates. Past studies have shown mature barley to be active ... Barley Grass Inhibits 73% of Leukemia Cells in Vitro: An extract of green barley grass (Hordeum vlgare L.) powder was shown to ...
Dr. Felix E Rivera-Mariani - Research Profile | Larkin University
Evaluation of the Cooperative Proinflammatory Effect between Endotoxin and (1-3)-β-D-glucan with the in-vitro Pyrogen Test. ... Cryopreserved human whole blood: a human-based in-vitro immunotoxicological system expanded into environmental health and ... Immunodetection of (1-3)-β-D-glucan with the Halogen Immunoassay. Poster Presentation. American Industrial Hygiene Association ...
Index by author - April 01, 1988, 86 (4) | Plant Physiology
β-Glucoside Activators of Mung Bean UDP-Glucose: β-Glucan Synthase Theresa Callaghan, Peter Ross, Patricia Weinberger-Ohana, ... In Vitro Synthesis and Processing of Tomato Fruit Polygalacturonase Dean DellaPenna, Alan B. Bennett ... β-Glucoside Activators of Mung Bean UDP-Glucose: β-Glucan Synthase Theresa Callaghan, Peter Ross, Patricia Weinberger-Ohana, ... β-Glucoside Activators of Mung Bean UDP-Glucose: β-Glucan Synthase Theresa Callaghan, Peter Ross, Patricia Weinberger-Ohana, ...
Publications | Biotechnology and Bioengineering
The effects of using this β-glucan as the sole carbon source for the in vitro growth of two probiotic bacteria (L. acidophilus ... 258 Effects of Ophiocordyceps dipterigena BCC 2073 β-Glucan as a Prebiotic on the in vitro Growth of Probiotic and Pathogenic ... The β-glucan produced by Ophiocordyceps dipterigena BCC 2073 is a (1, 3)-β-D-glucan with highly branching O-6-linkedside chains ... Keywords: Antimicrobial, probiotic, prebiotic, β-glucan, Ophiocordyceps dipterigena Procedia APA BibTeX Chicago EndNote Harvard ...
Ganoderma Lucidum | Digital Naturopath
Associate Professor Andrew Barnes - School of Biological Sciences - University of Queensland
D-glucans activate but do not prime teleost macrophages in vitro and are inactivated by dilute acid: Implications for dietary ... Mackintosh, Katrina, Dang, Cecile, Barnes, Andrew and Green, Timothy (2011). Effect of In-Vitro Pesticide Exposure On Oxidative ... Mackintosh, Katrina, Dang, Cecile, Barnes, Andrew and Green, Timothy (2011). In-vitro pesticide exposure on the oxidative burst ...
how to use barley powder for weight loss
"β-Glucan extracts inhibit the in vitro intestinal uptake of long-chain fatty acids and cholesterol and down-regulate genes ... The -glucan present in barley is prebiotic. You gotta workout and eat healthy. Barley is an ideal weight-loss food. New Delhi: ... glucan, that absorbs water to form a gel-like substance. The content is not intended to be a substitute for professional ...
The Health Benefits of Beta-Glucanase - Dr. Eddy Bettermann MD
These glucans also create up to 60% of the cell wall of many forms of fungal organisms such as Candida albicans and candidal ... Edward Group Beta-Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan. ... biofilm (the common experience of candida in the gut). Beta glucans… ... The build up of excess beta-glucans has also been linked to increased antibiotic resistance.. In-vitro and In-vivo studies on ...
Use of random forest analysis to quantify the importance of the structural characteristics of beta-glucans for prebiotic...
We performed a parallel in vitro fermentation of six native and carboxymethylated beta-glucans from mushroom, bacteria, seaweed ... We performed a parallel in vitro fermentation of six native and carboxymethylated beta-glucans from mushroom, bacteria, seaweed ... We performed a parallel in vitro fermentation of six native and carboxymethylated beta-glucans from mushroom, bacteria, seaweed ... We performed a parallel in vitro fermentation of six native and carboxymethylated beta-glucans from mushroom, bacteria, seaweed ...
CiNii Articles - Uchiyama Hirofumi
Improved β-glucan yield using an Aureobasidium pullulans M-2 mutant strain in a 200-L pilot scale fermentor targeting ... In vitro Culture of Unfertilized OVules in Solanum melongena and Ovaries in Zea mays （1971） ... A small scale study on the effects of oral administration of the β-glucan produced by Aureobasidium pullulans on milk quality ... In vitro Culture of Unfertilized OVules in Solanum melongena and Ovaries in Zea mays （1971） ...
Novel Strategy to Fight TB - Drug Discovery Opinion
The research validates inhibition of GlgE as therapy for TB but also highlights the potential for targeting two α-glucan ... killing the bacteria in vitro and in a mouse model of infection. Inhibition of another enzyme in the pathway was non-lethal ... The pathway involves four enzymatic steps in the conversion of the disaccharide, trehalose, to α-glucan mediated by TreS, Pep2 ... until combined with inactivation of Rv3032, a glucosyltransferase involved in a distinct α-glucan pathway. Inhibition of Rv3032 ...
Indian Journal of Pharmacology
Super Aqua Emulsion ⋅ Universal ⋅ GUERLAIN
PolysaccharidesFungalDigestionMacrophagesAbstractPolysaccharideViscosityProteinsSaccharomycesVivo and in vitroSolubleMoleculesReceptorInhibitorBarleyYeastCytokinesPure beta-glucanPhysicochemicalCandidaSynthasesCerealImmunomodulatorySubunitCelluloseExtracellularPleurotusInsolubleAlpha-glucansBeta-1,3-D-glucanIntestinalSolubilityOrally administeredConcentrationsStructuralBIOSYNTHESISGlucoseWater dikinaseFKS1Fermentation systemSignificantlyHydrolysisBiological responseDegradationMushroomSubstancesFecalPolymersMedicinalBacterialMolecularAlbicansImmunityModulateAntibodiesBone marrowAntitumorCholesterol
- The bioaccessibility of peptides and amino acids (AA) during in vitro gastrointestinal digestion of proteins from acid dairy gel (or yogurt) containing different polysaccharides (PS) was investigated. (gc.ca)
- The relationship of beta-glucan Mw (or intrinsic viscosity calculated from Mw), concentration and zero shear viscosity measured in the extracts closely resembled the general relationship reported for "random coil" polysaccharides with two distinct regions, below and above the occurrence of coil overlap. (nofima.no)
- These results indicate bioactive 6-linked glucans present in P. ostreatus extracts activate NK-cell cytotoxicity via regulation of activation and induction of IFNγ and NO. These studies establish a positive role for bioactive P. ostreatus polysaccharides in NK-cells activation and induction of an innate immune response against breast and lung cancer cells. (frontiersin.org)
- Zymosan is a yeast-derived particle composed principally of polysaccharides, of which β-glucan, the active component mediating the cellular effects ( 2 ), and mannan are the major constituents ( 3 ). (rupress.org)
- Beta-glucans are polysaccharides occurring in the bran of cereal grains, the cell wall of Saccharomyces cerevisiae and bacteria, certain types of fungi, seaweed and many kinds of mushrooms. (clinicaltrials.gov)
- α-Glucans (alpha-glucans) are polysaccharides of D-glucose monomers linked with glycosidic bonds of the alpha form. (wikipedia.org)
- It is critical for maintaining cell shape and integrity and contains the polysaccharides 1,3-β-glucan and chitin ( 4 , 38 ), which are not constituents of mammalian cells. (asm.org)
- β-Glucans are a group of biologically-active fibers or polysaccharides from natural sources with proven medical significance. (mdpi.com)
- Cereal β-glucans - including β-glucan from oat, barley and wheat - are linear polysaccharides joined by 1,3 and 1,4 carbon linkages. (wikipedia.org)
- Glucans are polysaccharides (structures comprised of linked sugars) that are found in many different foods. (whfoods.com)
- β‑glucans are linear unbranched polysaccharides found in various natural sources, such as mushrooms. (spandidos-publications.com)
- in particular, highly polymerized α-glucan polysaccharides, such as starch and glycogen, are the most common substrates in environmental niches. (asm.org)
- Due to the structural organization of highly polymerized α-glucans, bacteria require appropriate combinations of enzymes for depolymerization of the more complex polysaccharides to oligo- and monosaccharides. (asm.org)
- Among these enzymes are the pullulanases, which have glycosidase hydrolase activity toward α-glucan polysaccharides, considered to be key extracellular components in bacterial metabolism. (asm.org)
- Furthermore, fluorescence-activated scanning analysis and confocal imaging studies performed on whole bacteria indicate that the presence of α-glucan polysaccharides in culture medium upregulates the expression of SAP on the bacterial surface ( 31 ). (asm.org)
- It allows the unequivocal identification and direct structural characterization of the products synthesized in vitro, as opposed to conventional methods that rely on the use of radioactive substrates and enzymatic hydrolysis of the polysaccharides with specific glycoside hydrolases. (diva-portal.org)
- Numerous studies have reported that polysaccharides can stimulate the secretion of G-CSF in vivo and in vitro [ 14 - 16 ]. (hindawi.com)
- Candida albicans β-1-3,1-6-glucan (β-glucan), a pathogen-associated molecular pattern (PAMP) present in the fungal cell wall, has been characterized as a potent immune modulator. (frontiersin.org)
- C. albicans and fungal cell wall β-glucans induced functional reprogramming of monocytes, leading to enhanced cytokine production in vivo and in vitro. (nih.gov)
- Fungal sources contain a mixture of beta-1,3-glucan and beta-1,6-glucan. (grandstrandmed.com)
- We sought to determine whether Coccidioides posadasii possesses genes homologous to known fungal FKS genes that encode the catalytic subunit of 1,3-β-glucan synthase. (asm.org)
- 1,3-β-glucan is the predominant polysaccharide in most fungal cell walls, including those of Saccharomyces cerevisiae , Candida albicans , and Neurospora crassa ( 14 , 38 ), and is a significant component in the walls of C. posadasii in all growth phases ( 21 , 42 ). (asm.org)
- This systematic review aimed to compile and compare clinical studies using these [fungal] beta glucans as adjuvants on patients undergoing cancer treatment. (betaglucan.org)
- A recent study demonstrated that bacterial and fungal β-glucans stimulate IFN-β production by dendritic cells (DCs) following detection by the Dectin-1 receptor, but the effects of β-glucan-induced type I IFNs have not been defined. (jimmunol.org)
- We investigated whether type I IFNs regulate CD8 T cell activation by fungal β-glucan particle-stimulated DCs. (jimmunol.org)
- Additional negative tests included serum galactomannan and [beta]-d-glucan, serology for Cryptococcus, Histoplasma, Blastomyces and Coccidioides, CMV PCR, EBV PCR as well as fungal, mycobacterial and bacterial blood cultures. (thefreedictionary.com)
- Glycogen and starch synthesis involves elongation of α(1,4)-linked glucans by glycogen synthase (GS), an enzyme that uses UDP-glucose (Glc) or ADP-Glc as substrate donor in mammalian and fungal or in plant and bacterial cells, respectively ( 1 ). (pnas.org)
- Thus, this novel conjugate vaccine can efficiently immunize and protect against two major fungal pathogens by mechanisms that may include direct antifungal properties of anti-beta-glucan antibodies. (sigmaaldrich.com)
- Dectin-1 binds fungal beta-glucans and is involved in the innate immune responses to fungal pathogens [ PMID: 11470510 , PMID: 16341139 ]. (ebi.ac.uk)
- In vitro and in vivo studies indicated that fungal sterols down-regulated genes involved in cholesterol homeostasis (such as Srebf2 and Nr1h4 (FXR)) and other specific mushroom extracts (β-glucans and other water-soluble compounds) also stimulated transcriptional profiles similar to simvastatin or ezetimibe (two hypocholesterolemic drugs). (rsc.org)
- Yet arthritis can be elicited in an SPF environment through antigen-nonspecific activation of innate immunity, for example, by injection of zymosan, a crude fungal extract containing β-glucans, or purified β-glucans such as laminarin ( 5 ). (rupress.org)
- The impacts of molecular weight (MW), viscosity, and solubility of β-glucan on the rate of in vitro starch digestion and estimated glycemic index (GI) were evaluated. (biomedsearch.com)
- The in vitro starch hydrolysis of the mixtures and a control, white bread, increased as digestion time increased. (biomedsearch.com)
- As the MW of β-glucan increased, the starch hydrolysis decreased during in vitro digestion. (biomedsearch.com)
- These results illustrated the importance of viscosity attributed to β-glucan MW on starch hydrolysis during in vitro digestion. (biomedsearch.com)
- The present study aims to elucidate the structure and conformation of gently extracted oat β-glucan before and after in vitro gastric and gastrointestinal digestion utilizing asymmetric flow field-flow fractionation (AF4) and NMR spectroscopy. (lu.se)
- Oat β-glucan without digestion presented primary aggregates with fringed micelle structure, and other high molar mass supramolecular. (lu.se)
- Oat β-glucan was gently extracted from oat flour and its conformational characteristics before and after in vitro gastric and gastrointestinal digestion was investigated with AF4. (lu.se)
- Oat β-glucan without digestion presented a fringed micelle structure for primary aggregates of single β-glucan chains, and other supramolecular secondary aggregates of primary aggregates were detected for the high molar mass species. (lu.se)
- Several studies have shown that physicochemical properties of beta-glucan such as molecular weight (M) and solubility/extractability as well as the viscosity of food products after in vitro digestion are important for this physiological effect. (nofima.no)
- In this study, we directly compare beta-glucan physicochemical properties and flow behavior of different cereal products after analytical (thermostable alpha-amylase) and physiological (in vitro digestion) extractions. (nofima.no)
- The beta-glucan weight average molecular weights (Mw) were similar for both types of extractions, while differences in beta-glucan solubility between the food products were only evident after in vitro digestion. (nofima.no)
- The extracts after in vitro digestion behaved essentially as pure beta-glucan solutions. (nofima.no)
- Moreover, the capacity of a high-phytase yeast strain to hydrolyze phytate was investigated in an in vitro digestion model. (chalmers.se)
- In all porridges, β-glucan was continually degraded during digestion but was best preserved in the porridge made from malt prepared by steeping at 48°C with LA. A high-phytase Saccharomyces cerevisiae strain was studied at a simulated gastric pH gradient and intestinal pH values with digestive enzymes using growth medium and wheat gruel as model meals. (chalmers.se)
- Digestion Residues of Typical and High-β-Glucan Oat Flours Provide Sub" by Sedat Sayar, Jean-Luc Jannink et al. (iastate.edu)
- In vitro fermentabilities of the oat flour digestion residues (ODR) from two commercial oat lines with 4.7 and 5.3% β-glucan and from two high-β-glucan experimental lines with 7.6 and 8.1% β-glucan were evaluated and compared with fermentations of lactulose, purified oat β-glucan (POBG), and purified oat starch (POS). (iastate.edu)
- and White, Pamela J., "Digestion Residues of Typical and High-β-Glucan Oat Flours Provide Substrates for in Vitro Fermentation" (2007). (iastate.edu)
- Since β-glucan-trained monocytes have not been classified within the system of mononuclear phagocytes we analyzed these cells metabolically, phenotypically and functionally with a focus on monocyte-to-macrophage differentiation and compared them with naïve monocytes and other types of monocyte-derived cells such as classically (M1) or alternatively (M2) activated macrophages and monocyte-derived dendritic cells (moDCs). (frontiersin.org)
- We show that β-glucan inhibits spontaneous apoptosis of monocytes independent from autocrine or paracrine M-CSF release and stimulates monocyte differentiation into macrophages. (frontiersin.org)
- β-Glucan-differentiated macrophages exhibit increased cell size and granularity and enhanced metabolic activity when compared to naïve monocytes. (frontiersin.org)
- Our data demonstrate that β-glucan priming induces a population of immune competent long-lived monocyte-derived macrophages that may be involved in immunoregulatory processes. (frontiersin.org)
- Here, using novel and specific reagents, we have defined the receptors involved in the nonopsonic recognition of zymosan and soluble β-glucans in primary macrophages. (rupress.org)
- B-glucan nonoparticles can activate macrophages to produce immune enhancing cytokines (IL-1B, IL-6, TNF-alpha, IFN-y). (betaglucan.org)
- Hunter KW, Jr. Berner MD, Sura ME Alvea BN, " IFN-gamma primes macrophages for enhanced TNF-alpha expression in response to stimulatory and non-stimulatory amounts of microparticulate beta-glucan . (betaglucan.org)
- 3)-D-glucan (MG) from Saccharomyces cerevisiae for its ability to induce proinflammatory cytokine secretion in mouse peritoneal macrophages in vitro, and we have examined the effect of IFN-gamma. (betaglucan.org)
- Carp macrophages exhibit a trained immunity-like profile in vitro. (jimmunol.org)
- Both peptidoglycan and β-glucan can induce trained immunity in carp macrophages. (jimmunol.org)
- We established an in vitro model to study trained immunity in fish by adapting a well-described culture system of head kidney-derived macrophages of common carp. (jimmunol.org)
- A soluble NOD-specific ligand and a soluble β-glucan were used to train carp macrophages, after which cells were rested for 6 d prior to exposure to a secondary stimulus. (jimmunol.org)
- Transcriptomes (RNA-Seq) and epigenomes (ChIP-Seq H3K4me1,H3K4me3,H3K27ac) in four primary cell types: monocytes, in vitro differentiated naive, tolerized and trained macrophages were characterized. (ebi.ac.uk)
- The aim of this study was to determine, using murine RAW 264.7 macrophages, the immunomodulatory effect of extracellular β-glucan isolated from Pleurotus eryngii (PEBG) and its sulfated derivative (PEBG-S) on signaling molecules implicated in host innate immunity. (begellhouse.com)
- Abstract: b-glucan, an immunomodulator, can selectively enhance the immunobiological activities of neutrophils and macrophages without stimulating proinflammatory cytokine production. (scientific.net)
- Particulate yeast β-glucan is internalized by RAW 264.7 macrophages and reduces the activity of the tumor-associated protease legumain. (uio.no)
- On the other hand, there are macrophages that inhabit the intestinal wall and are able to pick up beta-1,3-D-glucan particles through beta-glucan receptors. (tldp.com)
- Schiefer, S, Lee, EYC & Whelan, WJ 1978, ' The requirement for a primer in the in vitro synthesis of polysaccharide by sweet-corn (1 → 4)-α-d-glucan syntrase ', Carbohydrate Research , vol. 61, no. 1, pp. 239-252. (elsevier.com)
- 1, 3- (Beta) -D-Glucan is a natural polysaccharide derived from the cell walls of baker,s yeast saccharomyces cerevsiae with immunoenhancing and potent antioxidant effects. (thefreelibrary.com)
- β-glucan is an important polysaccharide due to its medicinal properties of stimulating the immune system and preventing chronic diseases such as cancer. (biomedsearch.com)
- 2 Lentinan is a water-soluble, high molecular weight polysaccharide in a triple helix structure, containing only glucose molecules with mostly (1-3)-beta-D-glucan linkages in the regularly branched main chain with 2 beta (1,6)-D-glucopyranoside branchings for every 5 beta-(1,3)-glucopyranoside linear linkages. (drugs.com)
- In this study, we examined the effects of the oral (p.o.) administration of polysaccharide fractions extracted from S. crispa , using hot water (SCHWE), a β-glucan from S. crispa , to DBA/2 mice on cytokine induction by SCG in the spleen in vitro . (begellhouse.com)
- Beta 1,6-glucan, a protein bound polysaccharide, has been identified as the active constituent. (mskcc.org)
- A simple and sensitive method for the characterization of products synthesized in vitro by polysaccharide synthases is described. (diva-portal.org)
- The polysaccharide isolated from A. formosanus juice is a type II arabinogalactan with an average molecular weight of 29 kDa, and it is a prebiotic that increases the growth of probiotics in vivo and in vitro [ 6 ]. (hindawi.com)
- Detailed biochemical and enzymatic characterization of the GH5_4 modules of all three recombinant proteins confirmed particularly high specificities for the XyG polysaccharide versus a panel of other cell wall glycans, including mixed-linkage beta-glucan and cellulose. (biomedcentral.com)
- Impact of Molecular Weight, Viscosity, and Solubility of β-Glucan on In Vitro Oat Starch Digestibility. (biomedsearch.com)
- Viscosity increased and solubility decreased with an increase in MW of β-glucan. (biomedsearch.com)
- The estimated GI values were negatively correlated with β-glucan peak and final viscosity (r = -0.81, -0.82). (biomedsearch.com)
- So, the most interesting property of β-glucan that influences the physiological effects is that increases the viscosity inside the gastrointestinal. (lu.se)
- The lower the trimer-tetramer ratio, the higher the β-glucan viscosity in solution. (wikipedia.org)
- The yoghurt containing 0.3% β -glucan had the same viscosity as the control and the yoghurt containing 0.5% β -glucan had the lowest viscosity. (scirp.org)
- Rolled oats and isolated beta-glucan (termed oat gum in the report) contributed to increased gut viscosity in male Wistar rats [ 2 ]. (biomedcentral.com)
- Increased diet viscosity by oat β-glucans decreases the passage rate of liquids in the stomach and affects digesta physicochemical properties in growing pigs. (wur.nl)
- Western analysis of the medium proteins with β1,3-glucan- and β1,6-glucan-specific antibodies showed further that at least some of the released β1,3-glucan was linked to protein as part of a β1,3-glucan-β1,6-glucan-protein complex. (asm.org)
- These data indicate that Gas1p might play a role in the retention of β1,3-glucan and/or β-glucosylated proteins. (asm.org)
- In addition to the Rho1p regulatory subunit, other proteins are required for normal levels of β-1,3-glucan. (genetics.org)
- β -Glucans, isolated from Pleurotus citrinopileatus mushroom, were used in the manufacture of yoghurt from reduced fat cow milk with added proteins. (scirp.org)
- To identify starch-related enzymes that rely on glucan-bound phosphate, we studied the binding of proteins extracted from Arabidopsis wild-type leaves to either phosphorylated or nonphosphorylated starch granules. (plantphysiol.org)
- S. mutans makes several proteins that have the property of binding glucans. (omicsonline.org)
- We hypothesized that three of these glucan-binding proteins (Gbps), Gbps A, C and D, contribute to the cariogenicity of S. mutans. (omicsonline.org)
- It was concluded that, contrary to what happens in mammalian and yeast cells in which two different proteins are required for linear α(1,4)-linked glucan formation (glycogenin for initiation and GS for further elongation), in A. tumefaciens and probably in all other bacteria, the same protein is involved in both glycogen initiation and elongation. (pnas.org)
- 4)-[beta]-glucan synthase activities was based on the manipulation of digitonin concentrations used in the solubilization of membrane proteins. (plantphysiol.org)
- 1993. SKN1 and KRE6 define a pair of functional homologs encoding putative membrane proteins involved in beta-glucan synthesis. (rupress.org)
- Consistent with their direct role in the assembly of this polymer, both Kre6p and Skn1p possess COOH-terminal domains with significant sequence similarity to two recently identified glucan-binding proteins. (rupress.org)
- Large-scale screening of genetic and chemical-genetic interactions was used to examine the assembly and regulation of β-1,3-glucan in Saccharomyces cerevisiae . (genetics.org)
- Anticlastogenic effect of β-glucan, extracted from Saccharomyces cerevisiae, on cultured cells exposed to ultraviolet radiation. (biomedsearch.com)
- Therefore, these results indicate that β-glucan from Saccharomyces cerevisiae can be used in the prevention and/or reduction of DNA damage. (biomedsearch.com)
- B-glucan molecules with different chain lengths were extracted from yeast Saccharomyces cerevisiae and thereafter modified. (betaglucan.org)
- oral pretreatment with…Saccharomyces cerevisiae-beta-d-glucan once daily for 14 consecutive days significantly increased the survival rate of mice from 6 Gy X-rays irradiation. (betaglucan.org)
- Chemical shifts assignments of the α-mannan and β-glucan are presented as well as chemical shift values obtained from a (1 → 6)/(1 → 3)-β-d-glucan derived from Saccharomyces cerevisiae which was used as reference. (uio.no)
Vivo and in vitro3
- In conclusion, the botanic type II AGAF in this study was a potent G-CSF inducer in vivo and in vitro. (hindawi.com)
- 16 ] showed that β -glucan isolated from Grifola frondosa stimulates the production of G-CSF in vivo and in vitro. (hindawi.com)
- In-vivo and in-vitro studies indicate that β-glucans may augment the effects of some anti-tumor monoclonal antibodies and independently promote tumor regression (Hong et al. (ift.org)
- In vivo administration of zymosan, or purified soluble β-glucans, has a number of desirable effects on immune function, including the ability to confer resistance to tumors and various infections, prompting interest in the development of β-glucan-based therapeutics ( 4 , 5 ). (rupress.org)
- The term "beta-glucan" refers to a class of soluble fibers found in many plant sources. (grandstrandmed.com)
- Oat β-glucans are water-soluble β-glucans derived from the endosperm of oat kernels known for their cholesterol lowering and hypoglycemic properties, as well as their use in various cosmetic applications. (wikipedia.org)
- Cereal fibers that are high in water-soluble fiber, such as β-glucan, may improve cardiovascular disease risk through improvements in serum cholesterol and other intermediary risk factors. (biomedcentral.com)
- Soluble fiber β-glucan is thought to be the active component for the cholesterol lowering effect of oats. (biomedcentral.com)
- Soluble β-glucan preparation from the cold NaOH extract of Sparassis crispa (SCG) is a six-branched 1,3-β-D-glucan that is a major cell-wall structural component in fungi. (begellhouse.com)
- As there are two forms of β‑glucans, insoluble and soluble forms, they are able to interact with lipids and biliary salts in the bowel and consequently reduce cholesterol levels. (spandidos-publications.com)
- a water soluble (1 → 6)-linked β-d-glucan with single and short (1 → 3)-linked β-glucan side chains (ACcSw) and a less branched (1 → 3)-linked β-d-glucan (ACcIw/ACcId), proposed to contain short side chains in O-4. (uio.no)
- β1,3-Glucans are synthesized as linear molecules that are extruded into the periplasmic space. (asm.org)
- A variety of different studies on the health benefits of shiitake mushrooms have converged on a special group of carbohydrate-related molecules called glucans. (whfoods.com)
- Specifically, type I IFNs promote Ag presentation on MHC I molecules, CD86 and CD40 expression, and the production of IL-12 p70, IL-2, IL-6, and TNF-α by β-glucan-stimulated DCs. (jimmunol.org)
- Like hyaluronic acid, Hydrasensyl Glucan consists of a long chain of sugar molecules. (przoom.com)
- It has been shown to trigger epigenetic remodeling and metabolic reprogramming through a pathway involving dectin-1, the surface receptor of β-glucan, and the PI3K/Akt/mTOR (phosphoinositide 3-kinase/Akt/mechanistic target of rapamycin) signaling cascade ( 6 , 7 ). (frontiersin.org)
- Using specific carbohydrate inhibitors, we show that a β-glucan receptor, but not the MR, is a predominant receptor involved in this process. (rupress.org)
- Furthermore, nonopsonic zymosan binding was unaffected by genetic CD11b deficiency or a blocking monoclonal antibody (mAb) against CR3, demonstrating that CR3 was not the β-glucan receptor mediating this activity. (rupress.org)
- To address the role of the recently described β-glucan receptor, Dectin-1, we generated a novel anti-Dectin-1 mAb, 2A11. (rupress.org)
- These findings define Dectin-1 as the leukocyte β-glucan receptor, first described over 50 years ago, and resolves the long-standing controversy regarding the identity of this important molecule. (rupress.org)
- Early studies, using carbohydrate inhibitors to block various leukocyte receptors, suggested that the cellular recognition of unopsonized zymosan is mediated by the mannose receptor and a β-glucan receptor ( 6 - 8 ). (rupress.org)
- The identity of the β-glucan receptor, which has been defined as a β-glucan inhibitable receptor for particulate activators of the alternative complement pathway ( 6 ), is controversial. (rupress.org)
- The ability of CR3 to recognize β-glucans led to the proposal that this receptor is the major β-glucan receptor on leukocytes and that it mediates all the immunomodulatory effects of these carbohydrates, including the β-glucan-dependent binding of zymosan ( 4 , 9 - 11 ). (rupress.org)
- Conflicting evidence, however, indicated that another receptor(s) mediates this activity ( 6 , 12 - 14 ), and although we and others have identified additional receptors capable of recognizing β-glucans ( 15 - 17 ), their role in primary cells is unclear. (rupress.org)
- The main challenge in defining the contribution of these various receptors to the recognition of β-glucans has been the lack of receptor-specific reagents. (rupress.org)
- These studies suggest that Dectin-1 is the leukocyte β-glucan receptor, the identity of which has remained elusive since its first description over five decades ago. (rupress.org)
- The training required the β-glucan receptor dectin-1 and the noncanonical Raf-1 pathway. (nih.gov)
- Real-time polymerase chain reaction array revealed that the expression levels of nuclear factor-κB and Toll-like receptor signaling genes in cells were upregulated by PEBG and PEBG-S. Moreover, the expression of the β-glucan receptor dectin-2 was significantly upregulated by PEBG and PEBG-S treatment, reflecting immune activation through the dectin-2- Syk -(CARD9/Bcl-10/MALT1) pathway. (begellhouse.com)
- However, 3-β-D glucan synthase, thus increases hDL leukotriene receptor in practice. (imagenenaccion.org)
- Goldman, and later Czop, identified a specific receptor on the cells of macrophage origin that binds to the beta-1,3-D-glucan molecule. (tldp.com)
- In parallel, we screened the yeast deletion mutant collection for altered sensitivity to the glucan synthase inhibitor, caspofungin. (genetics.org)
- Here, we determined the virulence factors (germination, adherence, biofilm formation, phospholipase and proteinase production) of 16 C. auris isolates and their susceptibilities to 11 drugs belonging to different antifungal classes, including a novel orally bioavailable 1,3-β- d -glucan synthesis inhibitor (SCY-078). (asm.org)
- Most of the investigational agents are either azole derivatives or glucan inhibitor echinocandins. (asm.org)
- The novel oral glucan synthase inhibitor SCY-078 shows in-vitro activity against Candida spp. (escmid.org)
- Barley and beta-glucan isolated from barley lower total and LDL cholesterol. (greenmedinfo.com)
- Barley beta-glucan lowers cholesterol and increases fecal bile acid and short chain fatty acid secretion in mildly hypercholesteremic individuals. (greenmedinfo.com)
- The aim of this work was to optimize a malting procedure to degrade phytate and/or increase phytase activity in barley to improve iron availability and to preserve the content of β-glucan, since β-glucan is associated with health promoting properties and is therefore of nutritional interest. (chalmers.se)
- Fincher, Geoffrey 2004-10-07 00:00:00 A putative barley (1→3)-β-d-glucan synthase cDNA of 6.1 kb, which is homologous to the yeast FKS gene, was assembled from DNA fragments obtained through screening of barley cDNA and BAC libraries, and by PCR amplification. (deepdyve.com)
- A (1→3)-β-d-glucan synthase has been purified more than 60-fold from barley suspension-cultured cells by detergent extraction, CaCl2 treatment, sucrose density gradient centrifugation and non-denaturing gel electrophoresis. (deepdyve.com)
- Thus, the amino acid sequence predicted from the HvGSL1 gene has been linked with the actual amino acid sequence of an active (1→3)-β-d-glucan synthase fraction from barley. (deepdyve.com)
- β-glucans were first discovered in lichens, and shortly thereafter in barley. (wikipedia.org)
- In oats, β-glucan is found mainly in the endosperm of the oat kernel, especially in the outer layers of that endosperm (a marked difference from barley, which contains β-glucan uniformly throughout the endosperm). (wikipedia.org)
- studied the effects of addition of β-glucan from different cereal sources (oat and barley) on the growth and metabolic activity of probiotic organisms in yoghurt. (scirp.org)
- Brennan and Tudorica determined the possible beneficial effects of barley beta-glucan, as a fat substitute on the rheological, textural and sensory quality of low-fat yoghurt. (scirp.org)
- The proliferation of MC3T3-E1 osteoblastic cells was enhanced by treatment with Aureobasidium b-glucan, while neither mushroom b-glucan nor barley b-glucan increased the cell proliferation. (scientific.net)
- Cui X., Shin H., Song C., Laosinchai W., Amano Y.and Brown R.M.Jr.2001.A putative plant homolog of the yeast (1!3) b glucan synthase subunit FKS1 from cotton ( Gossypium hirsutum L.)fibers.Planta 213:223-230. (springer.com)
- Baker's yeast beta glucan supplementation increases salivary IgA and decreases cold/flu symptomatic days after intense exercise. (greenmedinfo.com)
- B-Glucan used was yeast derived Beta 1,3/1,6 glucan. (betaglucan.org)
- More than 30 years ago, Seljelid and co-workers screened a large number of glucans and glycans …for their macrophage-activating ability in vitro, and found that a particulate beta-1,3/1,6-glucan prepared from baker's yeast was the most active. (betaglucan.org)
- The in vitro activities of these agents against 104 isolates of yeast pathogens of Candida spp. (asm.org)
- 1991. Yeast beta-glucan synthesis: KRE6 encodes a predicted type II membrane protein required for glucan synthesis in vivo and for glucan synthase activity in vitro. (rupress.org)
- In Vitro Aflatoxin BBinding by the Cell Wall and (1→3)-β-d-Glucan of Baker's Yeast. (bioportfolio.com)
- The aim of this study was to evaluate the ability of heat-killed baker's yeast (HKBY), the cell wall of baker's yeast (CWBY), and cell wall (1→3)-β-d-glucan of baker's yeast (BGBY) to bind aflatoxi. (bioportfolio.com)
- β-1,3- and 1,6-glucans from fungi and yeast have numerous biological effects, including immunostimulation against infectious diseases and some forms of cancer. (ift.org)
- Although β-glucan-primed cells expressed markers of alternative activation and secreted higher levels of IL-10 after lipopolysaccharide (LPS), their capability to release pro-inflammatory cytokines and to kill bacteria was unaffected. (frontiersin.org)
- Leukocytes from DBA/2 mice are highly sensitive to SCG, producing cytokines in vitro . (begellhouse.com)
- Materials analyzed included: pure beta-glucan, Oatwell (commercially available oat-bran containing 22% oat β-glucan), xylooligosaccharides (XOS), WholeFiber (dried chicory root containing inulin, pectin, and hemi/celluloses), and pure inulin. (preprints.org)
- Oatwell's effect was similar to those of the pure beta-glucan samples, both samples promoted the highest mean propionate production at 24 h. (preprints.org)
- β-Glucan derived from cell walls of Candida albicans is a potent immune modulator. (frontiersin.org)
- SCH56592 is a new triazole derivative that has in vitro activity against Candida spp. (asm.org)
- SCY-078 exhibits potent inhibition of β-(1,3)- d -glucan synthesis, an essential cell wall component of many pathogenic fungi, including Candida spp. (asm.org)
- Passive protection was prevented by adsorption of antibodies on Candida cells or beta-glucan particles before transfer. (sigmaaldrich.com)
- Staphylococcus aureus, Eschericia coli, Candida albicans, Pneumocytis carinii, Listeria monococytogenesis, Leishmania donovani, Herpes simplex, Ascaris suum - this is an incomplete list of microorganisms, against which a protective effect of glucan has been established. (tldp.com)
- Cereal beta-glucans may reduce fasting LDL-cholesterol and post-prandial blood glucose rise in humans. (nofima.no)
- Pr i dag består min forskningsportefølje av (i) grunnleggende forskning på mekanismer ansvarlig for positive helseeffekter fra bygg, havre og gjærß-glucaner (ii) dynamisk in vitro modellering av cereal fordøyelse og assosiert glykemisk respons (iii) Anvendt forskning i samarbeid med industrien for å utvikle en industriell prosess som fjerner glukose fra frukt juice med tanke på å lage en ny klasse av sunn, funksjonell mat. (nofima.no)
- Marketed foods supplemented with hypocholesterolemic compounds are only inhibiting mechanisms for cholesterol absorption ( i.e. phytosterols and cereal β-glucans). (rsc.org)
- Furthermore, these results identify Dectin-1 as a new target for examining the immunomodulatory properties of β-glucans for therapeutic drug design. (rupress.org)
- At the current level of scientific understanding it is not possible to characterize the effects of beta-glucan more specifically than to say that it has "immunomodulatory" actions, or that it is a "biological response modifier. (grandstrandmed.com)
- Multiple approaches have led to the identification of a putative membrane-bound subunit of a β1,3-glucan synthase complex. (asm.org)
- An alternative subunit of the β1,3-glucan synthase complex was cloned by homology to FKS1 . (asm.org)
- The glucan synthase complex has been best studied in S. cerevisiae and consists of a catalytic subunit encoded by either of two genes ( FKS1 or FKS2 ) and a regulatory subunit ( RHO1 ). (asm.org)
- These findings suggest that a higher level of subunit organization is required for in vitro cellulose synthesis in comparison with callose assembly. (plantphysiol.org)
- The results presented show that the hybrid aspen is a promising model for in vitro studies on callose and cellulose synthesis. (springer.com)
- The x-ray diffraction pattern of the treated product favoring ß-1,4-glucan synthesis strongly resembled that of cellulose II. (utexas.edu)
- On the basis of product analysis, the positive identification of in vitro-synthesized cellulose was established. (utexas.edu)
- Role of (1,3)(1,4)-β-glucan in cell walls: interaction with cellulose. (semanticscholar.org)
- however, oat β-glucans do become insoluble above a certain concentration. (wikipedia.org)
- The ethanol insoluble fraction from this reaction contained ß-1,4- glucan and ß-1,3-glucan in an approximate ratio of 25:69. (utexas.edu)
- On the basis of methylation analysis, the acetic/nitric acid reagent-insoluble glucan product was found to be exclusively ß-1,4-linked. (utexas.edu)
- Out of different glucans, the beta-1,3-D-glucan configuration has been shown to act as a non-specific immune-activator. (tldp.com)
- When a macrophage encounters beta-1,3-D-glucan, it becomes activated. (tldp.com)
- Although most of the research with this substance has been done in vitro and parenterally, later research at Baylor College of Medicine, sponsored by ImmuDyne, indicates the oral effectiveness of purified beta-1,3-D-glucan (Wyde, 1989). (tldp.com)
- The integrity of beta-1,3-D-glucan taken orally differs from other food substances. (tldp.com)
- Studies conducted with oral application of C13 labeled glucan also support existence of phagocytic transport for beta-1,3-D-glucan. (tldp.com)
- An adjuvant concept of pharmacological application for beta-1,3-D-glucan was suggested by DiLuzio in the 70s. (tldp.com)
- This article is an attempt to overview this concept from today's perspectives utilizing modern knowledge of oral effectiveness, and a specific transport mechanism of beta-1,3-D-glucan. (tldp.com)
- There is now enough data to support the use of beta-1,3-D-glucan as an adjuvant in several important medicinal applications. (tldp.com)
- Beta-1,3-D-glucan itself can elicit broad anti-infective effects. (tldp.com)
- Unlike an antibiotic compound interfering with metabolism of a pathogen, beta-1,3-D-glucan is a substance that modifies host response to cells genetically different from the host. (tldp.com)
- The aim of this study is to determine the effect of the potential prebiotic beta-glucan on the intestinal microflora, the bacterial enzyme activity (beta-glucuronidase and beta-glucosidase) in the feces, the fecal pH and the concentration of fecal short-chain fatty acid in polypectomized patients. (clinicaltrials.gov)
- To observe the effect of beta-glucan on intestinal symptoms and well-being. (clinicaltrials.gov)
- We demonstrated beneficial effects of B-glucan on intestinal barrier function and increased B-glucan-passage through FAE model. (betaglucan.org)
- We also compared the fermentability of concentrated oat β-glucan with inulin and guar gum in a model intestinal fermentation system. (biomedcentral.com)
- To estimate colonic fermentability, 0.5 g concentrated oat β-glucan was incubated in a batch model intestinal fermentation system, using human fecal inoculum to provide representative microflora. (biomedcentral.com)
- Based on a model intestinal fermentation, this oat β-glucan was fermentable, producing higher amounts of butyrate than other fibers. (biomedcentral.com)
- In vitro disposition studies to assess solubility, intestinal permeability, and metabolic stability were predictive of good oral bioavailability. (asm.org)
- In Europe, several health claim requests were submitted to the EFSA NDA Panel (Dietetic Products, Nutrition and Allergies), related to the role of β-glucans in maintenance of normal blood cholesterol concentrations and maintenance or achievement of a normal body weight. (wikipedia.org)
- In July 2009, the Scientific Committee issued the following statements: On the basis of the data available, the Panel concludes that a cause-and-effect relationship has been established between the consumption of beta-glucans and the "reduction of blood cholesterol concentrations. (wikipedia.org)
- The following wording reflects the scientific evidence: "Regular consumption of beta-glucans contributes to maintenance of normal blood cholesterol concentrations. (wikipedia.org)
- Concentrated oat β-glucan was a fermentable fiber and produced total SCFA and acetate concentrations similar to inulin and guar gum. (biomedcentral.com)
- Concentrated oat β-glucan produced the highest concentrations of butyrate at 4, 8, and 12 hours. (biomedcentral.com)
- β-Glucan was extracted and purified from the mycelial culture using optimal medium concentrations. (begellhouse.com)
- Proliferation of aHDF was measured at various concentrations of β-glucan by MTT assay, and migration was observed for 36h on microscope. (scientific.net)
- The structural features and the bile acid-binding capacity of oat β-glucan were probed with NMR. (lu.se)
- Three structural classes of (1→3)-β- d -glucans are encountered in some important soil-dwelling, plant-associated or human pathogenic bacteria. (springer.com)
- 1,3-β-Glucan synthase is responsible for the synthesis of β-glucan, an essential cell wall structural component in most fungi. (asm.org)
- Structural characterization of a branched (1 → 6)-α-mannan and β-glucans isolated from the fruiting bodies of Cantharellus cibarius. (uio.no)
- β-Glucan can also enhance the blood glucose levels and blood pressure. (lu.se)
- An important component of the cell wall is the glucose polymer β1,3-glucan. (asm.org)
- The FKS genes encode large polypeptides (∼200 kDa) with 16 membrane-spanning domains that synthesize 1,3-β-glucan from UDP-glucose subunits ( 4 , 11 , 33 ). (asm.org)
- Sucrose-dependent adherence is mediated by glucans, polymers of glucose synthesized from sucrose by glucosyltransferase (Gtf) enzymes. (omicsonline.org)
- As any other glucan (or polyglucose), it consists of glucose units linked together. (tldp.com)
- Glucan, Water Dikinase Exerts Little Control over Starch Degradation in Arabidopsis Leaves at Night. (jic.ac.uk)
- The first step on the pathway of starch degradation in Arabidopsis (Arabidopsis thaliana) leaves at night is the phosphorylation of starch polymers, catalyzed by glucan, water dikinase (GWD). (jic.ac.uk)
- Disruption of FKS1 induces the expression of FKS2 , which is responsible for the residual glucan synthase activity in an fks1 Δ strain ( 35 ). (asm.org)
- Using the set of deletion mutants in ∼4600 nonessential genes, we scored synthetic interactions with genes encoding subunits of the β-1,3-glucan synthase ( FKS1, FKS2 ), the glucan synthesis regulator ( SMI1 / KNR4 ), and a β-1,3-glucanosyltransferase ( GAS1 ). (genetics.org)
- Deletion of FKS1 leads to a decrease in β-glucan and an increase in chitin and mannoprotein levels in the cell wall. (genetics.org)
- The objective of this research project was to compare potential prebiotic effects and fermentability of five commonly consumed fibers using an in vitro fermentation system measuring changes in fecal microbiota, total gas production and formation of common SCFAs. (preprints.org)
- Substrates were fermented by using an in vitro batch fermentation system under anaerobic conditions for 24 h. (iastate.edu)
- Oat β-glucan produced significant reduction from baseline in total cholesterol (-0.3 ± 0.1 mmol/L) and LDL cholesterol (-0.3 ± 0.1 mmol/L), and the reduction in LDL cholesterol were significantly greater than in the control group (p = 0.03). (biomedcentral.com)
- Six grams concentrated oat β-glucan per day for six weeks significantly reduced total and LDL cholesterol in subjects with elevated cholesterol, and the LDL cholesterol reduction was greater than the change in the control group. (biomedcentral.com)
- Thus, a practical dose of β-glucan can significantly lower serum lipids in a high-risk population and may improve colon health. (biomedcentral.com)
- This in vitro study illustrates that C. albicans should not be disregarded in healthy oral ecosystems, as it has the potential to influence bacteria significantly. (pubmedcentralcanada.ca)
- The in vitro estimated GI of the mixture without β-glucan, determined from the starch hydrolysis rate, was 88.3 for Jim and 80.0 for N979, which decreased to 68.4 and 66.8, respectively, with the inclusion of high-MW β-glucan. (biomedsearch.com)
- The in vitro β-glucans were identified by hydrolysis of radioactive products, using specific enzymes. (springer.com)
- Usually β-glucan is solubilized in the extraction process with residual starch, which is then removed by hydrolysis with alpha-amylase. (wikipedia.org)
- Enzymic hydrolysis confirmed that the product was hydrolyzed only by cellobiohydrolase I. Autoradiography proved that the product was synthesized in vitro. (utexas.edu)
- In vitro enzymatic protein hydrolysis kinetics of feed ingredients. (wur.nl)
- The group B streptococcus type I pullulanase (SAP) is a class 13 glycoside hydrolase that is anchored to the bacterial cell surface via a conserved C-terminal anchoring motif and involved in α-glucan degradation. (asm.org)
- It has been suggested that GWD is important for the control of starch degradation, because its transcript levels undergo strong diel fluctuations, its activity is subject to redox regulation in vitro, and starch degradation is strongly decreased in gwd mutant plants. (jic.ac.uk)
- Reishi mushroom contains complex sugars known as beta-glucans. (mskcc.org)
- Mushroom b-glucan alone or associated with BMP-7 increased alkaline phosphatase (ALP) activity of MC3T3-E1 cells, one of the osteoblast phenotype markers, but the other b-glucans did not affect ALP activity of the cells. (scientific.net)
- The edible mushroom Albatrellus ovinus contains a α-L-fuco-α-D-galactan, α-D-glucan, a branched (1→6)-β-D-glucan and a branched (1→3)-β-D-glucan. (uio.no)
- Also the in vitro anti-cyto- and anti-genotoxicity of fecal water will be examined on human cell culture (HT29). (clinicaltrials.gov)
- To measure the effect of beta-glucan on fecal microflora (number of bacteria, bacterial enzyme activity, SCFA, pH). (clinicaltrials.gov)
- Fecal donors were not involved with the β-glucan feeding trial. (biomedcentral.com)
- Linear (1→3)-β-glucans and side-chain-branched (1→3,1→2)-β-glucans are major constituents of capsular materials, with roles in bacterial aggregation, virulence and carbohydrate storage. (springer.com)
- This review includes information on the structure, properties and molecular genetics of the bacterial (1→3)-β-glucans, together with an overview of the physiology and biotechnology of curdlan production and applications of this biopolymer and its derivatives. (springer.com)
- To generate a vaccine to protect against a variety of human pathogenic fungi, we conjugated laminarin (Lam), a well-characterized but poorly immunogenic beta-glucan preparation from the brown alga Laminaria digitata, with the diphtheria toxoid CRM197, a carrier protein used in some glyco-conjugate bacterial vaccines. (sigmaaldrich.com)
- The presence of C. albicans alters the bacterial microbiome in early in vitro oral biofilms, resulting in the presence of strictly anaerobic bacteria under oxygen-rich conditions. (pubmedcentralcanada.ca)
- In contrast, the bile acids induced alterations to the apparent density of the oat β-glucan aggregates indicating a molecular interaction which was further confirmed with NMR by observing numerous changes in the resonance of bile acids' carbons in presence of oat β-glucan. (lu.se)
- Steeping at 48°C gave small changes in the content and molecular weight of β-glucan. (chalmers.se)
- Furthermore, in vitro re-exposure of human PBMC to C. albicans induced a significant IL-6 and TNF-α response, even after a resting period of 6 d following primary exposure to C. albicans . (jimmunol.org)
- Activation of the Dectin-1/RAF proto-oncogene serine/threonine-protein kinase (Raf-1) pathway in PBMCs by β-glucans present in the cell wall of C. albicans proved crucial for inducing trained immunity ( 2 , 3 ). (jimmunol.org)
- S cerevisiae-beta glucan protects bone marrow cells from radiation damage through scavenging bone marrow cell reactive oxygen species (ROS), mitigating bone marrow cell DNA damage and improving cell cycle progress, and thus mitigated myelosuppression induced by irradiation and stimulated hematopoiesis, ultimately increased the survival of radiated mice. (betaglucan.org)
- The extensive research studying various effects of glucans on bone marrow showed significant restoration of both lymphopenia and neutropenia. (betaglucan.org)
- In other studies, maitake demonstrated antitumor effects (16) , enhanced bone marrow colony formation, reduced doxorubicin toxicity (11) , and inhibited tumor metastasis in vitro (13) . (mskcc.org)
- 3) glucan sulfate exerts antitumor therapeutic activity, and (4) glucan sulfate immunoprophylaxis will modify the course of experimental infectious disease. (curehunter.com)
- Animal and in vitro studies show significant antitumor and antimetastatic activity of lentinan 5 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 increased longevity 3 and synergism with other chemotherapeutic agents. (drugs.com)
- It is proven that a daily consumption of 3 grams of β-glucan can effectively reduce the blood cholesterol levels and prevent cardiovascular disease, which causes the death of thousands of people annually. (lu.se)
- It is thought that beta-glucan reduces cholesterol levels by increasing excretion of cholesterol from the digestive tract. (grandstrandmed.com)
- The current review discusses the evidence supporting the effects of β‑glucans on cholesterol levels. (spandidos-publications.com)