A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
A species of MORBILLIVIRUS causing distemper in dogs, wolves, foxes, raccoons, and ferrets. Pinnipeds have also been known to contract Canine distemper virus from contact with domestic dogs.
Established cell cultures that have the potential to propagate indefinitely.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions.
A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It is closely related to SHIGA TOXIN produced by SHIGELLA DYSENTERIAE.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
The type species of MORBILLIVIRUS and the cause of the highly infectious human disease MEASLES, which affects mostly children.
Proteins found in any species of virus.
Inoculation of a series of animals or in vitro tissue with an infectious bacterium or virus, as in VIRULENCE studies and the development of vaccines.
Process of growing viruses in live animals, plants, or cultured cells.
Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.
A species of the genus FLAVIVIRUS which causes an acute febrile and sometimes hemorrhagic disease in man. Dengue is mosquito-borne and four serotypes are known.
A protein phytotoxin from the seeds of Ricinus communis, the castor oil plant. It agglutinates cells, is proteolytic, and causes lethal inflammation and hemorrhage if taken internally.
The lone species of the genus Asfivirus. It infects domestic and wild pigs, warthogs, and bushpigs. Disease is endemic in domestic swine in many African countries and Sardinia. Soft ticks of the genus Ornithodoros are also infected and act as vectors.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It shares 50-60% homology with SHIGA TOXIN and SHIGA TOXIN 1.
A class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS. They include SHIGA TOXIN which is produced by SHIGELLA DYSENTERIAE and a variety of shiga-like toxins that are produced by pathologic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157.
A family of RNA viruses naturally infecting rodents and consisting of one genus (ARENAVIRUS) with two groups: Old World Arenaviruses (ARENAVIRUSES, OLD WORLD) and New World Arenaviruses (ARENAVIRUSES, NEW WORLD). Infection in rodents is persistent and silent. Vertical transmission is through milk-, saliva-, or urine-borne routes. Horizontal transmission to humans, monkeys, and other animals is important.
The use of techniques that produce a functional MUTATION or an effect on GENE EXPRESSION of a specific gene of interest in order to identify the role or activity of the gene product of that gene.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A family of large icosahedral DNA viruses infecting insects and poikilothermic vertebrates. Genera include IRIDOVIRUS; RANAVIRUS; Chloriridovirus; Megalocytivirus; and Lymphocystivirus.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
A genus of FLAVIVIRIDAE containing several subgroups and many species. Most are arboviruses transmitted by mosquitoes or ticks. The type species is YELLOW FEVER VIRUS.
A name for several highly contagious viral diseases of animals, especially canine distemper. In dogs, it is caused by the canine distemper virus (DISTEMPER VIRUS, CANINE). It is characterized by a diphasic fever, leukopenia, gastrointestinal and respiratory inflammation and sometimes, neurologic complications. In cats it is known as FELINE PANLEUKOPENIA.
Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.
A subgroup of the genus FLAVIVIRUS which comprises a number of viral species that are the etiologic agents of human encephalitis in many different geographical regions. These include Japanese encephalitis virus (ENCEPHALITIS VIRUS, JAPANESE), St. Louis encephalitis virus (ENCEPHALITIS VIRUS, ST. LOUIS), Murray Valley encephalitis virus (ENCEPHALITIS VIRUS, MURRAY VALLEY), and WEST NILE VIRUS.
Immunoglobulins produced in response to VIRAL ANTIGENS.
A plant family of the order Plumbaginales, subclass Caryophyllidae, class Magnoliopsida of shrubs and herbs. Some members contain ANTHOCYANINS and naphthaquinones.
The functional hereditary units of VIRUSES.
A family of viruses, mainly arboviruses, consisting of a single strand of RNA. Virions are enveloped particles 90-120 nm diameter. The complete family contains over 300 members arranged in five genera: ORTHOBUNYAVIRUS; HANTAVIRUS; NAIROVIRUS; PHLEBOVIRUS; and TOSPOVIRUS.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Arthropod-borne viruses. A non-taxonomic designation for viruses that can replicate in both vertebrate hosts and arthropod vectors. Included are some members of the following families: ARENAVIRIDAE; BUNYAVIRIDAE; REOVIRIDAE; TOGAVIRIDAE; and FLAVIVIRIDAE. (From Dictionary of Microbiology and Molecular Biology, 2nd ed)
Ribonucleic acid that makes up the genetic material of viruses.
The type (and only) species of RUBIVIRUS causing acute infection in humans, primarily children and young adults. Humans are the only natural host. A live, attenuated vaccine is available for prophylaxis.
A genus of gram-negative, aerobic, rod-shaped bacteria often surrounded by a protein microcapsular layer and slime layer. The natural cycle of its organisms generally involves a vertebrate and an invertebrate host. Species of the genus are the etiological agents of human diseases, such as typhus.
A plant genus of the family ASTERACEAE. The common name of thoroughwort is also used for other plants including EUPATORIUM; CHROMOLAENA, Hebeclinium and Koanophyllon. Eupolin is the aqueous extract of the leaves.
The type species of the FLAVIVIRUS genus. Principal vector transmission to humans is by AEDES spp. mosquitoes.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A species of CORONAVIRUS causing infections in chickens and possibly pheasants. Chicks up to four weeks old are the most severely affected.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Proteins that are coded by immediate-early genes, in the absence of de novo protein synthesis. The term was originally used exclusively for viral regulatory proteins that were synthesized just after viral integration into the host cell. It is also used to describe cellular proteins which are synthesized immediately after the resting cell is stimulated by extracellular signals.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
The type species of RUBULAVIRUS that causes an acute infectious disease in humans, affecting mainly children. Transmission occurs by droplet infection.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
A genus of protozoan parasites of the subclass COCCIDIA. Its species are parasitic in dogs, cattle, goats, and sheep, among others. N. caninum, a species that mainly infects dogs, is intracellular in neural and other cells of the body, multiplies by endodyogeny, has no parasitophorous vacuole, and has numerous rhoptries. It is known to cause lesions in many tissues, especially the brain and spinal cord as well as abortion in the expectant mother.
Deoxyribonucleic acid that makes up the genetic material of viruses.
A phenomenon in which infection by a first virus results in resistance of cells or tissues to infection by a second, unrelated virus.
A species of CORONAVIRUS causing atypical respiratory disease (SEVERE ACUTE RESPIRATORY SYNDROME) in humans. The organism is believed to have first emerged in Guangdong Province, China, in 2002. The natural host is the Chinese horseshoe bat, RHINOLOPHUS sinicus.
Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.
A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE), which is the etiological agent of Japanese encephalitis found in Asia, southeast Asia, and the Indian subcontinent.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
Substances elaborated by viruses that have antigenic activity.
A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.
Specific hemagglutinin subtypes encoded by VIRUSES.
Proteins encoded by a VIRAL GENOME that are produced in the organisms they infect, but not packaged into the VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A toxin produced by SHIGELLA DYSENTERIAE. It is the prototype of class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS.
Glycosphingolipids which contain as their polar head group a trisaccharide (galactose-galactose-glucose) moiety bound in glycosidic linkage to the hydroxyl group of ceramide. Their accumulation in tissue, due to a defect in ceramide trihexosidase, is the cause of angiokeratoma corporis diffusum (FABRY DISEASE).
A phenomenon manifested by an agent or substance adhering to or being adsorbed on the surface of a red blood cell, as tuberculin can be adsorbed on red blood cells under certain conditions. (Stedman, 25th ed)
A family of the order DIPTERA that comprises the mosquitoes. The larval stages are aquatic, and the adults can be recognized by the characteristic WINGS, ANIMAL venation, the scales along the wing veins, and the long proboscis. Many species are of particular medical importance.
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
A subfamily in the family CEBIDAE that consists of four genera: CALLITHRIX (marmosets), CALLIMICO (Goeldi's monkey), LEONTOPITHECUS (lion tamarins), and SAGUINUS (long-tusked tamarins). The members of this family inhabit the tropical forests of South and Central America.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
A plant species of the genus MELIA, family MELIACEAE, which is toxic to insects. The name is very similar to Melia azadirachta (AZADIRACHTA).
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
The type species of PNEUMOVIRUS and an important cause of lower respiratory disease in infants and young children. It frequently presents with bronchitis and bronchopneumonia and is further characterized by fever, cough, dyspnea, wheezing, and pallor.
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
Multinucleated masses produced by the fusion of many cells; often associated with viral infections. In AIDS, they are induced when the envelope glycoprotein of the HIV virus binds to the CD4 antigen of uninfected neighboring T4 cells. The resulting syncytium leads to cell death and thus may account for the cytopathic effect of the virus.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A group of acute infections caused by herpes simplex virus type 1 or type 2 that is characterized by the development of one or more small fluid-filled vesicles with a raised erythematous base on the skin or mucous membrane. It occurs as a primary infection or recurs due to a reactivation of a latent infection. (Dorland, 27th ed.)
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Vaccines or candidate vaccines used to prevent infection with Japanese B encephalitis virus (ENCEPHALITIS VIRUS, JAPANESE).
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A rare, slowly progressive encephalitis caused by chronic infection with the MEASLES VIRUS. The condition occurs primarily in children and young adults, approximately 2-8 years after the initial infection. A gradual decline in intellectual abilities and behavioral alterations are followed by progressive MYOCLONUS; MUSCLE SPASTICITY; SEIZURES; DEMENTIA; autonomic dysfunction; and ATAXIA. DEATH usually occurs 1-3 years after disease onset. Pathologic features include perivascular cuffing, eosinophilic cytoplasmic inclusions, neurophagia, and fibrous gliosis. It is caused by the SSPE virus, which is a defective variant of MEASLES VIRUS. (From Adams et al., Principles of Neurology, 6th ed, pp767-8)
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
An analog of DEOXYURIDINE that inhibits viral DNA synthesis. The drug is used as an antiviral agent.
Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.

Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (1/5424)

Herpes simplex virus type 1 (HSV-1) has many attractive features that suggest its utility for gene transfer to neurons. However, viral cytotoxicity and transient transgene expression limit practical applications even in the absence of viral replication. Mutant viruses deleted for the immediate early (IE) gene, ICP4, an essential transcriptional transactivator, are toxic to many cell types in culture in which only the remaining IE genes are expressed. In order to test directly the toxicity of other IE gene products in neurons and develop a mutant background capable of longterm transgene expression, we generated mutants deleted for multiple IE genes in various combinations and tested their relative cytotoxicity in 9L rat gliosarcoma cells, Vero monkey kidney cells, and primary rat cortical and dorsal root neurons in culture. Viral mutants deleted simultaneously for the IE genes encoding ICP4, ICP22 and ICP27 showed substantially reduced cytotoxicity compared with viruses deleted for ICP4 alone or ICP4 in combination with either ICP22, ICP27 or ICP47. Infection of neurons in culture with these triple IE deletion mutants substantially enhanced cell survival and permitted transgene expression for over 21 days. Such mutants may prove useful for efficient gene transfer and extended transgene expression in neurons in vitro and in vivo.  (+info)

Rubella virus-induced apoptosis varies among cell lines and is modulated by Bcl-XL and caspase inhibitors. (2/5424)

Rubella virus (RV) causes multisystem birth defects in the fetuses of infected women. To investigate the cellular basis of this pathology, we examined the cytopathic effect of RV in three permissive cell lines: Vero 76, RK13, and BHK21. Electron microscopy and the TUNEL assay showed that the cytopathic effect resulted from RV-induced programmed cell death (apoptosis) in all three cell lines, but the extent of apoptosis varied among these cells. At 48 h postinfection, the RK13 cell line showed the greatest number of apoptotic cells, the Vero 76 cell line was approximately 3-fold less, and BHK21 had very few. An increased multiplicity of infection and longer time postinfection were required for the BHK21 cell line to reach the level of apoptotic cells in Vero 76 at 48 h. Purified RV induced apoptosis in a dose-dependent fashion, but not UV-inactivated RV or virus-depleted culture supernatant. Specific inhibitors of the apoptosis-specific proteases caspases reduced RV-induced apoptosis and led to higher levels of RV components in infected cells. To address the role of regulatory proteins in RV-induced apoptosis, the antiapoptotic gene Bcl-2 or Bcl-XL was transfected into RK13 cells. Although a high level of Bcl-2 family proteins was expressed, no protection was observed from apoptosis induced by RV, Sindbis virus, or staurosporine in RK13 cells. In BHK21 cells, however, increased expression of Bcl-XL protected cells from apoptosis. The observed variability in apoptotic response to RV of these cell lines demonstrates that programmed cell death is dependent on the unique properties of each cell and may be indicative of how selective organ damage occurs in a congenital rubella syndrome fetus.  (+info)

CLIP-170 highlights growing microtubule ends in vivo. (3/5424)

A chimera with the green fluorescent protein (GFP) has been constructed to visualize the dynamic properties of the endosome-microtubule linker protein CLIP170 (GFP-CLIP170). GFP-CLIP170 binds in stretches along a subset of microtubule ends. These fluorescent stretches appear to move with the growing tips of microtubules at 0.15-0.4 microm/s, comparable to microtubule elongation in vivo. Analysis of speckles along dynamic GFP-CLIP170 stretches suggests that CLIP170 treadmills on growing microtubule ends, rather than being continuously transported toward these ends. Drugs affecting microtubule dynamics rapidly inhibit movement of GFP-CLIP170 dashes. We propose that GFP-CLIP170 highlights growing microtubule ends by specifically recognizing the structure of a segment of newly polymerized tubulin.  (+info)

Susceptibilities of Mycobacterium tuberculosis and Mycobacterium avium complex to lipophilic deazapteridine derivatives, inhibitors of dihydrofolate reductase. (4/5424)

Twelve lipophilic 2,4-diamino-5-methyl-5-deazapteridine derivatives and trimethoprim were evaluated for activity against Mycobacterium tuberculosis and Mycobacterium avium in vitro. Six of the compounds had MICs of < or =12.8 mg/L and < or =1.28 mg/L against M. tuberculosis and M. avium, respectively; trimethoprim MICs were >128 mg/L and >12.8 but < or =128 mg/L, respectively. Two compounds, with either a 2-methyl-5-methoxy phenyl or 2-methoxy-5-trifluoromethyl phenyl linked at the 6-position of the deazapteridine moiety by a CH2NH bridge, had MICs of < or =0.13 mg/L against M. avium; the two compounds also had apparent I50 values for dihydrofolate reductase of 2 and 8 nM, respectively, compared with an I50 of 400 nM with trimethoprim. Four of the compounds were selectively toxic to mycobacteria as compared with Vero cells. These results demonstrated that lipophilic antifolates can be synthesized which are more active against mycobacteria than trimethoprim and which possess selective toxicity.  (+info)

Mutations in the retinoblastoma protein-binding LXCXE motif of rubella virus putative replicase affect virus replication. (5/5424)

The rubella virus (RV)-encoded protein NSP90, which contains the retinoblastoma protein (Rb)-binding motif LXCXE, interacts with Rb and RV replication is reduced in cells lacking Rb. Whether the LXCXE motif of RV NSP90 itself is essential for Rb binding and virus replication is not known. Therefore, in the present study, the functional role of this motif was investigated by site-directed mutagenesis in a plasmid from which infectious RV RNA can be produced. Three critical mutations in the motif, two substitutions at the conserved cysteine residue (C --> G and C --> R) and a deletion of the entire motif, were created. A cell-free translated NSP90 C terminus polypeptide containing the deletion did not bind to Rb and a polypeptide carrying the C --> R substitution had barely detectable binding affinity for Rb. Rb binding by the C --> G mutant was reduced significantly compared to that of wild-type protein. Correlating with the binding results, mutant viruses containing the LXRXE and LXGXE motifs had a reduction in replication to < 0.5% and 47% of the wild-type, respectively, while deletion of the motif was found to be lethal. By the first serial passage, replication of the LXRXE-carrying virus had increased from < 0.5% to 2% of the wild-type. Sequencing of the genome of this virus revealed a nucleotide change that altered the motif from LXRXE to LXSXE, which is a known Rb-binding motif in two protein phosphatase subunits. Thus, our results clearly demonstrate that the LXCXE motif is required for efficient RV replication.  (+info)

Characterization of the L gene and 5' trailer region of Ebola virus. (6/5424)

The nucleotide sequences of the L gene and 5' trailer region of Ebola virus strain Mayinga (subtype Zaire) have been determined, thus completing the sequence of the Ebola virus genome. The putative transcription start signal of the L gene was identical to the determined 5' terminus of the L mRNA (5' GAGGAAGAUUAA) and showed a high degree of similarity to the corresponding regions of other Ebola virus genes. The 3' end of the L mRNA terminated with 5' AUUAUAAAAAA, a sequence which is distinct from the proposed transcription termination signals of other genes. The 5' trailer sequence of the Ebola virus genomic RNA consisted of 676 nt and revealed a self-complementary sequence at the extreme end which may play an important role in virus replication. The L gene contained a single ORF encoding a polypeptide of 2212 aa. The deduced amino acid sequence showed identities of about 73 and 44% to the L proteins of Ebola virus strain Maleo (subtype Sudan) and Marburg virus, respectively. Sequence comparison studies of the Ebola virus L proteins with several corresponding proteins of other non-segmented, negative-strand RNA viruses, including Marburg viruses, confirmed a close relationship between filoviruses and members of the Paramyxovirinae. The presence of several conserved linear domains commonly found within L proteins of other members of the order Mononegavirales identified this protein as the RNA-dependent RNA polymerase of Ebola virus.  (+info)

The herpes simplex virus type 1 regulatory protein ICP27 is required for the prevention of apoptosis in infected human cells. (7/5424)

The herpes simplex virus type 1 (HSV-1) ICP27 protein is an immediate-early or alpha protein which is essential for the optimal expression of late genes as well as the synthesis of viral DNA in cultures of Vero cells. Our specific goal was to characterize the replication of a virus incapable of synthesizing ICP27 in cultured human cells. We found that infection with an HSV-1 ICP27 deletion virus of at least three separate strains of human cells did not produce immediate-early or late proteins at the levels observed following wild-type virus infections. Cell morphology, chromatin condensation, and genomic DNA fragmentation measurements demonstrated that the human cells died by apoptosis after infection with the ICP27 deletion virus. These features of the apoptosis were identical to those which occur during wild-type infections of human cells when total protein synthesis has been inhibited. Vero cells infected with the ICP27 deletion virus did not exhibit any of the features of apoptosis. Based on these results, we conclude that while HSV-1 infection likely induced apoptosis in all cells, viral evasion of the response differed among the cells tested in this study.  (+info)

Glycoprotein gL-independent infectivity of pseudorabies virus is mediated by a gD-gH fusion protein. (8/5424)

Envelope glycoproteins gH and gL, which form a complex, are conserved throughout the family Herpesviridae. The gH-gL complex is essential for the fusion between the virion envelope and the cellular cytoplasmic membrane during penetration and is also required for direct viral cell-to-cell spread from infected to adjacent noninfected cells. It has been proposed for several herpesviruses that gL is required for proper folding, intracellular transport, and virion localization of gH. In pseudorabies virus (PrV), glycoprotein gL is necessary for infectivity but is dispensable for virion localization of gH. A virus mutant lacking gL, PrV-DeltagLbeta, is defective in entry into target cells, and direct cell-to-cell spread is drastically reduced, resulting in only single or small foci of infected cells (B. G. Klupp, W. Fuchs, E. Weiland, and T. C. Mettenleiter, J. Virol. 71:7687-7695, 1997). We used this limited cell-to-cell spreading ability of PrV-DeltagLbeta for serial passaging of cells infected with transcomplemented virus by coseeding with noninfected cells. After repeated passaging, plaque formation was restored and infectivity in the supernatant was observed. One single-plaque isolate, designated PrV-DeltagLPass, was further characterized. To identify the mutation leading to this gL-independent infectious phenotype, Southern and Western blot analyses, radioimmunoprecipitations, and DNA sequencing were performed. The results showed that rearrangement of a genomic region comprising part of the gH gene into a duplicated copy of part of the unique short region resulted in a fusion fragment predicted to encode a protein consisting of the N-terminal 271 amino acids of gD fused to the C-terminal 590 residues of gH. Western blotting and radioimmunoprecipitation with gD- and gH-specific antibodies verified the presence of a gDH fusion protein. To prove that this fusion protein mediates infectivity of PrV-DeltagLPass, cotransfection of PrV-DeltagLbeta DNA with the cloned fusion fragment was performed, and a cell line, Nde-67, carrying the fusion gene was established. After cotransfection, infectious gL-negative PrV was recovered, and propagation of PrV-DeltagLbeta on Nde-67 cells produced infectious virions. Thus, a gDH fusion polypeptide can compensate for function of the essential gL in entry and cell-to-cell spread of PrV.  (+info)

The purpose of this research was to study the morphological features of cells and test the porcine follicular fluid (pFF) supplements in Vero cell lines culture. The pFF in the estrous cycle was collected and kept sterile properly. The fluid was categorized into three types: small size (1-3 mm), medium size (4-6 mm) and large size (,7 mm) follicles. In the control group, Vero cell lines were cultured in dulbeccos modified eagle medium (DMEM). In the positive control group, Vero cell lines were cultured in DMEM and supplemented using 10% heat-treated fetal bovine serum (HTFBS). In the third group, Vero cell lines were cultured in DMEM and supplemented using pFF from small, medium and large sized ovarian follicles at 2, 4, 20, 40, 200, 400, 500 and 600 μg protein/mL concentrations for 24 h, using MTT assay. The results related to pFF from small-, medium- and large-sized follicles indicated that the percentage of cell viability in tested group at 400-600 µg protein/mL showed the percentage of ...
BioAssay record AID 216212 submitted by ChEMBL: Evaluated in vitro for antiviral activity in vero cell cultures against Sindbis virus.
Parasite international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology
Video articles in JoVE about encephalitis virus western equine include Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors, Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay, Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses, Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids, In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus, Viral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems, Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain, High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum, High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale
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Elements of the cytoskeletal and microtubule networks were imaged in the Vero cell culture illustrated in this section by treating the fixed and permeabilized cells with mouse anti-alpha-tubulin primary antibodies, followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568.
This line is a clone of VERO 76 (ATCC CRL-1587). It was cloned by the dilution method into microtiter plates in 1979 by P.J. Price.- Plaques are also produced.
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Before DNA testing is done, mosquito samples sent to the Texas Department of State Health Services will be run through a Vero cell culture. In this method mosquito juice is squeezed onto a specially prepared plate with a thin layer of living cells. After a few days of incubating the cells with the mosquito juice, a technician can look at the cells under magnification and determine if they look healthy. The presence of sick cells tells the technician that the mosquito pool carried one of several viruses that attack these cells. The health department prefers the Vero cell culture method because it is sensitive to many kinds of mosquito borne viruses. When a sample is positive for virus, then one of the molecular tests for specific virus RNA is run. These tests are highly specific, so that a test for WNV will not detect say St. Louis encephalitis (SLE) or chikungunya (CHIK), other important mosquito-borne diseases. Several tests will be run on any cell culture positive sample of mosquito juice. The ...
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BioAssay record AID 336244 submitted by ChEMBL: Antiviral activity against 10-3 MOI HSV1 infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect.
Statement of the Problem: Parvoviral diseases are emerging as a constant threat to penaeid culture due to their ability to cause slow growth and mass mortality...
Pak1 is required for ASFV entry in Vero cells.A) ASFV activates Pak1 at early times post infection. Cells were infected (MOI 5) and phosphorylation of Pak1 (Thr
Does anyone has ever heard of macropinocytosis events or macropinocytosis stimulators usefull with Vero cells? Thanxs Rose-may Delrue URBM / FUNDP Lab Immunol. & Microbiol. 61, rue de Bruxelles B-5000 Namur Belgium ...
Vero cells are used in the development of Covid-19 vaccines Vero cells are used a lot in research and industry. Time to have a closer look at this cell line: Vero cells are continuous cell lines that was derived from the kidney of an African green monkey. ...
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Bio-imaging and drug carriers for delivery have created a huge demand for crystals. Crystals are fascinating materials that have been grown for a long time but obtaining biocompatible fluorescent crystals is a challenging task. We report on the growth of fluorescent crystals using a carbon dot (C-dot) solution by a hydrothermal process. The crystallization pattern of these C-dots exhibited a unique dendritic structure having a feather-like morphology. The growth temperature and pressure were maintained at 60℃ and 200 mmHg, respectively, for crystal growth. A green fluorescence (under UV light) that was observed in the C-dot solution was retained in the crystals formed from the solution. Cytotoxicity studies on Vero cells revealed the crystals to be extremely biocompatible. These fluorescent crystals are extremely well suited for biomedical and optoelectronic applications. ...
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With coronavirus/COVID-19 still a health issue, we want you to know that we are continuing to take extreme infection control measures in our office to maximize cleanliness and keep the environment as sanitary and safe as possible. This also means that only patients will be permitted to come into our office and everyone will have their temperature checked.. In an abundance of caution, we are requesting that if you are experiencing a fever, cough, chills, fatigue or any type of respiratory illness, please delay any upcoming appointments at our practice. We can talk with you by phone or email to establish a new date and time for the appointment. You may also want to contact your healthcare provider for further evaluation.. We do offer Virtual Consultations and Telemedicine through doxy.me. This is best used for specific situations including postoperative questions and as a substitute for some postoperative office visits relating to certain procedures. However, if you are planning to proceed with ...
TY - JOUR. T1 - Adaptation of yellow fever virus 17D to Vero cells is associated with mutations in structural and non-structural protein genes. AU - Beasley, David W.C.. AU - Morin, Merribeth. AU - Lamb, Ashley R.. AU - Hayman, Edward. AU - Watts, Douglas M.. AU - Lee, Cynthia K.. AU - Trent, Dennis W.. AU - Monath, Thomas P.. PY - 2013/9/1. Y1 - 2013/9/1. N2 - Serial passaging of yellow fever virus 17D in Vero cells was employed to derive seed material for a novel inactivated vaccine, XRX-001. Two independent passaging series identified a novel lysine to arginine mutation at amino acid 160 of the envelope protein, a surface-exposed residue in structural domain I. A third passage series resulted in an isoleucine to methionine mutation at residue 113 of the NS4B protein, a central membrane spanning region of the protein which has previously been associated with Vero cell adaptation of other mosquito-borne flaviviruses. These studies confirm that flavivirus adaptation to growth in Vero cells can ...
in Journal of Virology (1993), 67(7), 4379-4385. Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a protein with a predicted molecular weight of 51,540 presenting amino acid sequence homology with the immediate-early regulatory protein ... [more ▼]. Varicella-zoster virus (VZV) open reading frame 4 (ORF4) encodes a protein with a predicted molecular weight of 51,540 presenting amino acid sequence homology with the immediate-early regulatory protein ICP27 of herpes simplex virus type 1. To investigate the regulatory properties of the ORF4 gene product, we performed a series of transient expression assays in Vero cells, using a plasmid expressing ORF4 as effector and several VZV genes and heterologous genes as targets. The VZV target plasmids contained promoter/regulatory regions from genes belonging to the three putative VZV kinetic classes fused to the chloramphenicol acetyltransferase (CAT) gene. The heterologous target plasmids consisted of promoter/regulatory regions of ...
Two Cellmatics (Difco) Herpes simplex virus (HSV) detection systems, one with mink lung cells (ML) and the other with primary rabbit kidney cells (PRK) and the Cultureset (Ortho) system with Vero cells were compared for their ability to detect previously positive specimens. One hundred fifty patients specimens positive for HSV in either Vero or PRK cells prior to freezing at -70 degrees C were thawed once, diluted to 1:8, and inoculated in duplicate into each cell system. Positive cultures were detected using each kits immunoperoxidase method. All three cell lines were stained at 24 hr followed by a final staining of negative cultures at 48 hr for Cultureset and at 72 hr for Cellmatics as per kit instructions. At 24 hr percent detection was: Cellmatics (ML) = 85%, Cellmatics (PRK) = 81%, Cultureset (Vero) = 74%. At final staining percent detection was 94%, 92%, and 84%, respectively. The Cellmatics system has the advantage of fewer steps in the staining procedure and no manipulation of coverslips
The Vero+4 with Touchless Technology meets the rapidly changing needs of HORECA establishments for enhanced sanitary protocols, while delivering all the benefits of the popular Vero +4 system, said David Deshe, President and Co-Founder of Vero Water. The innovative sensor technology significantly reduces the need for wait staff to touch buttons and surfaces while filling Vero Water bottles. In an office environment or in hospitality venues with guest-facing shared amenity self-serve stations, touchless technology provides an additional level of safety. The VERO+4 dispenses exceptionally great tasting still and sparkling water-on site and on demand-swiftly, profitably, and sustainably by transforming tap water through its proprietary purification system and serving in eco-friendly reusable bottles. Vero takes pride in our ability to deliver a sustainable product that benefits the environment, adding touchless technology is another innovation that that will assist our clients to deliver our ...
According to Mikovits, one contaminated cell line is the Vero monkey kidney cell line called Vero E6, which was given by Fort Detrick - a U.S. Army Medical Command installation that hosts many of our national biological defense programs and houses the National Cancer Institute laboratory where she used to work - to the biosafety 4 laboratory (BSL-4) in Wuhan, China. This cell line is what the Wuhan lab used to grow and study coronaviruses, she says.. The Vero cell line is listed in the 2015 paper,8 A SARS-like Cluster of Circulating Bat Coronaviruses Shows Potential for Human Emergence, co-written by University of North Carolina researchers and Dr. Shi Zhengli, a Chinese virologist at the Wuhan lab who in 2010 published a paper9 discussing the weaponization of the SARS virus.. The contaminated Vero monkey kidney cells were also used in the production of polio vaccines, Mikovits notes. The original polio vaccines were passed through mice brains, as we didnt have cell lines in the 1930s when ...
De novo and reference-guided assembly.De novo assembly of the short reads was performed to generate new HSV-1 genomes from the sequence data, followed by a reference-guided assembly of the resulting blocks of contiguous sequences, or contigs. The short sequence reads were first passed through a series of computational filters that removed (i) mononucleotide sequences, (ii) host sequence contamination, and (iii) low-quality sequence. For step i, sequences that consisted of a single nucleotide or a single nucleotide with some N (noncalled) bases were removed. (Step ii) Since virus stocks were prepared on Vero cells, it was critical to identify and remove host DNA sequences. Because the vervet monkey (Vero cell parent) genome sequence is not known, the sequence data were mapped to the human genome (version 36) using the Mapping and Alignment with Qualities (MAQ) software package (32). Sequences homologous to human DNA varied from 0.2 to 15% of the data (see Table S1 in the supplemental material); ...
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Theodore G. Andreadis is the author of this article in the Journal of Visualized Experiments: Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
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"Baxter Sells Vero Cell Vaccines Platform". Genetic Engineering & Biotechnology News. 35 (2). 15 Dec 2014. p. 9. Note: The ... Baxter sold its vaccine production technology based on Vero cell culture to Nanotherapeutics. The sale included developed ... The new facility, once complete, will add cell therapy capabilities to Ology Bioservices' offerings. In mid-2016, ...
"Arenoviruses in Vero Cells: Ultrastructural Studies". Journal of Virology. 6 (4): 507-518. PETERS, C. J.; KUEHNE, R. W.; ...
It is produced in cultures of Vero cells. Modified vaccinia Ankara (also known as MVA): a highly attenuated (not virulent) ... On the other hand, the CEV is believed to play a role in cell-to-cell spread and the EEV is thought to be important for long ... Cell Stem Cell. 7 (5): 618-630. doi:10.1016/j.stem.2010.08.012. PMC 3656821. PMID 20888316. Heilprin, John (1 September 2007 ... He was attempting to grow vaccinia virus on agar media in the absence of living cells when he noted that many colonies of ...
Just subB is sufficient to cause vacuolation of vero cells. Neu5GC is not made by humans but is acquired from food sources such ... After their B subunit binds to receptors on the cell surface, the toxin is enveloped by the cell and transported inside either ... Once StxB targets a cancerous cell, it delivers the A subunit of the toxin which eventually kills the cancerous cell. Play ... SubAB's target is in the endoplasmic reticulum of the cell and is brought into the cell through clathrin-mediated endocytosis. ...
It is readily grown in monolayers of eukaryotic Vero cells. Serological evidence and PCR indicate that S. negevensis is ...
... to increase the receptivity of Vero cells. Silencing its effect with siRNA prevented infection of Vero cells. TIM1 is expressed ... the cells survived and appeared impervious to the virus, further indicating that Ebola relies on NPC1 to enter cells; mutations ... focus on cell death". Cell Death and Differentiation. 22 (8): 1250-1259. doi:10.1038/cdd.2015.67. ISSN 1350-9047. PMC 4495366. ... surface peplomer and is endocytosed into macropinosomes in the host cell. To penetrate the cell, the viral membrane fuses with ...
The fusion vectors are transfected in Vero cells (monkey kidney fibroblasts). Particularly interesting ORFs are also screened ... At the end of the live cell imaging, the cells can still be fixed and colocalisation experiments made. As the sequence of the ... The GFP-cDNA project documents the localisation of proteins to subcellular compartments of the eukaryotic cell applying ... and expressed in eukaryotic cells. Subsequently, the subcellular localisation of the fusion proteins is recorded by ...
... purified chicken embryo cell vaccine (PCECV), developed in 1984; and a purified Vero cell rabies vaccine (PVCRV) developed in ... These include the human diploid cell vaccine (HDCV) first introduced in 1978; ...
In Vero cells and LLC-MK2 cells, Lebombo virus causes lysis; however, it does not cause any cytopathic effect in C6/36 cells. ...
Newer vaccines, based on vero cells, are in development (as of 2018). The outbreak of yellow fever in Angola in January 2016 ... Others have noted that switching manufacturing processes to modern cell-culture technology might improve vaccine supply ... people with thymus disorders associated with abnormal immune cell function, people with primary immunodeficiencies, and anyone ...
... also had activity against SARS CoV2 in Vero E6 cells, although the mechanism of action is not yet known. It is the ... Ambroxol and ciprofloxacin have activity against SARS CoV2 in Vero E6 cells. Bradfute et al BioRxiv https://www.biorxiv.org/ ... Both ambroxol and its parent drug bromhexine have been shown to induce autophagy in several cell types, and ambroxol was shown ... Seifart C, Clostermann U, Seifart U, Müller B, Vogelmeier C, von Wichert P, Fehrenbach H (February 2005). "Cell-specific ...
A follow-up study indicates that ciprofloxacin has potent effects on inhibiting SARS-CoV-2 in Vero E6 cells as measured by ... www.cell.com/developmental-cell/fulltext/S1534-5807(16)30568-8; and in J. Cell Biol., available here (Kimura et al., JCB 2015) ... www.cell.com/molecular-cell/fulltext/S1097-2765(18)30190-4 (Chauhan et al., 2015) http://www.cell.com/molecular-cell/abstract/ ... Ambroxol is another drug that has beneficial effects in Vero E6 cells. The functional roles of galectins in cellular response ...
It is a mesophilic bacteria that can be grown on Vero cells. Similar to other Chlamydiales, it is commonly found in two ... Parachlamydia acanthamoebae has been shown to infect and multiply in simian and human cells. Experimental models were able to ... Their results also shows that the P. acanthamoebae cells continued to replicate within the macrophages, and eventually caused ... These researchers collected human macrophages and placed these cells on growth plates in conjunction with P. acanthamoebae. The ...
The vaccine candidate is produced with Bharat Biotech's in-house vero cell manufacturing platform that has the capacity to ... April 2018). "Enhancing viral vaccine production using engineered knockout vero cell lines - A second look". Vaccine. 36 (16): ... 2 was isolated by India's National Institute of Virology and used to grow large quantities of the virus using vero cells. From ... The study showed that Phase II trials had a higher immune response and induced T-cell response due to the difference in dosing ...
Vero cells are a continuous cell line derived from epithelial cells of the African green monkey kidney, and are widely used for ... "Vero cell platform in vaccine production: moving towards cell culture-based viral vaccines". Expert Review of Vaccines. 8 (5): ... Similar cell lines include buffalo green monkey kidney and BS-C-1. Chlorocebus monkeys are an important model organism for ... The African green monkey has been the focus of much scientific research since the 1950s, and cell lines derived from its ...
The Parachlamydiaceae naturally infect amoebae and can be grown in cultured Vero cells. The Parachlamydiaceae are not ...
1992). "Evaluation of Vero cell co-culture system for mouse embryos in various media". Human Reproduction. 7 (2): 276-80. doi: ... He also co-cultured human embryos with helper cells to promote growth. Cohen is known for the application of micromanipulation ...
It is based on a SA14-14-2 strain and cultivated in Vero cells. In September 2012, the Indian firm Biological E. Limited ... The other was an inactivated vaccine cultivated on primary hamster kidney cells (the Beijing-3 strain). The Beijing-3 strain ... launched an inactivated cell culture derived vaccine based on SA 14-14-2 strain which was developed in a technology transfer ...
"Inhibition of Dengue Virus Serotypes 1 to 4 in Vero Cell Cultures with Morpholino Oligomers". J. Virol. 79 (8): 5116-28. doi: ... HIV most heavily targets the immune system's white blood cells known as "helper T cells", and identifies these target cells ... HIV infects a cell through fusion with the cell membrane, which requires two different cellular molecular participants, CD4 and ... Release of viral genes and possibly enzymes into the host cell. Replication of viral components using host-cell machinery. ...
It is an inactivated whole virus vaccine, grown in culture using the Vero cell line. VLA2001 is currently undergoing a Phase I/ ...
Vero cells)". Chinese Clinical Trial Registry. Retrieved 15 August 2020. Xia S, Zhang Y, Wang Y, Wang H, Yang Y, Gao GF, et al ...
... the cells that constitute the blood-brain barrier). Vero cells have been suggested as a possible cheaper and faster alternative ... and with an abnormally large cell nucleus. On average, a Balamuthia trophozoite is about 30 to 120 μm in diameter. The cysts ... consisting of a standard complement of organelles surrounded by a three-layered cell wall (thought to be made of cellulose), ... Balamuthia must be cultured on primate hepatocytes or human brain microvascular endothelial cells ( ...
"Vitaherpavac is the first Russian herpes simplex virus vaccine obtained on the Vero B continuous cell line". Voprosy ... only the HSV-tk transfected Hela cells were killed by the granciclovir, leaving the nonviral cells unharmed. The Hela cells ... When Hela cells were transfected with the HSV-tk gene, and were then put in a culture with nontransfected cells, ... The miRNAs effect on apoptosis has affected cancer development by the regulation of cell proliferation, as well as cell ...
... because of its ability to kill Vero cells (African green monkey kidney cells) in culture. Shortly after, the verotoxin was ... Verocytotoxins/verotoxins - a seldom-used term for Stx - is from the hypersensitivity of Vero cells to Stx. The term Shiga-like ... In this way only the tumor cells, but not healthy cells, are destroyed during therapy. The toxin has two subunits-designated A ... The toxin requires highly specific receptors on the cells' surface in order to attach and enter the cell; species such as ...
The virus is shown to have a cytopathic effect on both human (HeLa) and monkey (vero) cells. As part of the minimal research ... Though the virus has never been a cause of illness in humans, it has the ability to infect both human and monkey cells, and its ... It has been proven to produce titers in human and monkey cells seventy-two hours after inoculation. Although it was discovered ... effects are seen, in both cell lines, 72 hours after contact. It was first discovered and studied by the Center for Zoonoses ...
Vero) cells by reverse genetics". Journal of Virology. 78 (4): 1851-7. doi:10.1128/JVI.78.4.1851-1857.2004. PMC 369478. PMID ... Inside the target cell, the two "stacked" Pol I and Pol II enzymes transcribe the viral cDNA to synthesize both negative-sense ... Morpholinos are effective in systems ranging in complexity from cell-free translation in a test tube to in vivo studies in ... However, the viral genes are still expressed in the host's cell through a single replication cycle, allowing for the ...
"Effect of acyclovir on the deoxyribonucleoside triphosphate pool levels in Vero cells infected with herpes simplex virus type 1 ... Deoxyguanosine triphosphate (dGTP) is a nucleoside triphosphate, and a nucleotide precursor used in cells for DNA synthesis. ...
"Avian reovirus triggers autophagy in primary chicken fibroblast cells and Vero cells to promote virus production". Archives of ... dsRNA inside of a cell is a tip off to the immune system that the cell is infected with a virus, since dsRNA does not occur in ... Uncoating and Entry Once inside the host cell, the virus must find a way to uncoat. The virus particles enter the cell in a ... It is unknown how they exit the host cell, but it thought that this is done once the host cell has died and disintegrated, ...
... isolated in Vero E6 cells from Peromyscus maniculatus trapped in the residence of the same case), only 16 nucleotide changes ... It had been assumed that in the process of adaptation to cell culture, selection of SNV variants which grow optimally in cell ... and subsequent adaptation to growth in Vero E6 cells. Additional viral strains have also been isolated from P. maniculatus ... of SNV has subsequently been determined by using RNA extracted from autopsy material as well as RNA extracted from cell culture ...
IIL operates a manufacturing facility in Ooty to manufacture the Vero cell culture rabies vaccine for use in human beings. This ...
Newer vaccines, based on vero cells, are in development and should replace 17D at some point.[4] ... The viruses infect, amongst others, monocytes, macrophages, and dendritic cells. They attach to the cell surfaces via specific ... After entering the host cell, the viral genome is replicated in the rough endoplasmic reticulum (ER) and in the so-called ... After transmission from a mosquito, the viruses replicate in the lymph nodes and infect dendritic cells in particular. From ...
Ita vero, error est verbum Latinum; erroros tamen non est. IacobusAmor (disputatio) 15:30, 11 Novembris 2017 (UTC). Videbam ... Res geographicae is fine, but these and other permutations are sometimes too much for the little gray cells to keep straight- ... Spare moments are few & far between these days (aside from times when coffee is being sipped and the little gray cells are ... but the textual hiccups in question have elicited from the little gray cells about as much as they're likely to, at least for ...
Cepivo 17D pa je še zmeraj v uporabi, razvijajo pa cepivo na osnovi celic vero, ki naj bi nekoč nadomestilo 17D.[10] ... TNF-alpha and NK cells activity". Virology 345 (1): 22-30. PMID 16278000. doi:10.1016/j.virol.2005.09.058.. CS1 vzdrževanje: ...
ACAM2000s virus is cultured in kidney epithelial cells (Vero cells) from an African green monkey. Efficacy and adverse reaction ... the virus seemed to move from cell to cell, but by around the 12th day, lysis of many infected cells occurred and the virus was ... Poxviruses are unique among DNA viruses in that they replicate in the cytoplasm of the cell rather than in the nucleus. In ... The life cycle of poxviruses is complicated by having multiple infectious forms, with differing mechanisms of cell entry. ...
HeLa cell apoptosis caused by CDV follows a different mechanism than that in vero cell lines.[83] This change in the caspase ... HeLa cells are an immortalized cancer cell line used frequently in research. The cell line was established by removing cells ... leading to cell death. Cell death in organisms is necessary for the normal development of cells and the cell cycle maturation.[ ... HeLa cell[edit]. Apoptosis in HeLa[b] cells is inhibited by proteins produced by the cell; these inhibitory proteins target ...
"Unlockd raises $12M to offer ad-funded discounts on your cell phone bill". Tech Crunch. Retrieved January 5, 2017 ...
O virus Ebola sobre a superficie dunha célula epitelial de ril de mono verde africano da liña celular Vero. ... "The spatio-temporal distribution dynamics of Ebola virus proteins and RNA in infected cells". Nature 3: 1206. Bibcode ...
ita vero. thus indeed. A useful phrase, as the Romans had no word for "yes", preferring to respond to questions with the ... and thus outside of a living organism or cell. Alternative experimental or process methodologies include in vitro, in silico, ...
Breaking Point i Hell in a Cell[75][76][77]. Dobę później po Hell in a Cell na Raw, Miz otrzymał kolejny rewanż o mistrzostwo, ... Nie zdołał odzyskać Intercontinental Championship w walce z Kingstonem na gali Hell in a Cell i 6 listopada na SmackDown[153][ ... Miz i Truth wtargnęli do klatki Hell in a Cell podczas tytułowej gali pay-per-view, gdzie zaatakowali Alberto Del Rio, CM Punka ... Chris Sokol, Sokol, Brian: Title changes highlight Hell in a Cell. W: Slam! Sports [on-line]. Canadian Online Explorer, October ...
HeLa cell apoptosis caused by CDV follows a different mechanism than that in vero cell lines.[82] This change in the caspase ... HeLa cells are an immortalized cancer cell line used frequently in research. The cell line was established by removing cells ... leading to cell death. Cell death in organisms is necessary for the normal development of cells and the cell cycle maturation.[ ... Removal of dead cellsEdit. The removal of dead cells by neighboring phagocytic cells has been termed efferocytosis.[63] Dying ...
"Dopo 400mila anni, ecco il vero volto dell'Uomo di Ceprano". Retrieved 26 October 2017.. ...
The first unofficial standard for the breed was introduced by an Englishman Vero Shaw in 1882.[39] In 1890 with the formation ... gene for a uric acid transporter that allows for uric acid to enter liver cells and be subsequently broken down is not present ...
"Virological and Serological Findings in Rousettus aegyptiacus Experimentally Inoculated with Vero Cells-Adapted Hogan Strain of ... the cells survived and appeared immune to the virus, further indicating that Ebola relies on NPC1 to enter cells. This might ... Two independent studies reported in the same issue of Nature showed that Ebola virus cell entry and replication requires NPC1.[ ... The same studies described similar results with Marburg virus, showing that it also needs NPC1 to enter cells.[49][50] ...
Florida City to Vero Beach, Florida, and Lake Okeechobee 09:00 UTC Tropical Storm Watch Florida City to Englewood, Florida, ... Much of this information was relayed from trapped victims via the SMS functions of their cell phones, to friends and relatives ... There is evidence that many prisoners were abandoned in their cells during the storm, while the guards sought shelter. Hundreds ...
Sebastian-Vero Beach, FL Metropolitan Statistical Area. Okeechobee, FL Micropolitan Statistical Area ... is the nation's only National Science Foundation-funded Industry/University Cooperative Research Center for Fuel Cells.[7] ...
Marburgvirus isolation is usually performed by inoculation of grivet kidney epithelial Vero E6 or MA-104 cell cultures or by ... The marburgvirus life cycle begins with virion attachment to specific cell-surface receptors, followed by fusion of the virion ... These particles are surrounded by a lipid membrane derived from the host cell membrane. The membrane anchors a glycoprotein (GP ... The most abundant protein produced is the nucleoprotein, whose concentration in the cell determines when L switches from gene ...
... cell tower triangulation, cellular signal strength and surrounding wifi signals.[11][12] ...
The virus grows readily on Vero cells and LLC-MK2 cells.[14] ... MERS cases have been reported to have low white blood cell ... "Protein & Cell. 3 (11): 803-5. doi:10.1007/s13238-012-2811-1. PMC 4875465. PMID 23143870.. ... "Protein & Cell. 4 (4): 248-50. doi:10.1007/s13238-013-2841-3. PMC 4875521. PMID 23549610.. ...
Virus raste brez težav na Celicah Vero in na celicah LLC-MK2.[17] ... Cell Host Microbe 16(3):328-337. doi: 10.1016/j.chom.2014.08.009 ... and maintains broad replicative capability in mammalian cell ...
"Analysis of Filovirus Entry into Vero E6 Cells, Using Inhibitors of Endocytosis, Endosomal Acidification, Structural Integrity ... "Efficacy of epidermal growth factor receptor inhibitors versus chemotherapy as second-line treatment in advanced non-small-cell ... "Identification of an env-defective HIV-1 mutant capable of spontaneous reversion to a wild-type phenotype in certain T-cell ...
... vero cell line), which is chemically inactivated with formalin.[20] After two doses of inactivated poliovirus vaccine (given by ... Follicular dendritic cell sarcoma. Extranodal NK/T-cell lymphoma, nasal type. MCPyV Merkel-cell carcinoma. RNA virus. HCV ... The virus then hijacks the host cell's own machinery, and begins to replicate. Poliovirus divides within gastrointestinal cells ... if the affected nerve cells are completely destroyed, paralysis will be permanent; cells that are not destroyed, but lose ...
At night the prisoners were crowded in small cells without room to lie down.. ... Sebastian-Vero Beach. *Tallahassee. *Tampa-St. Petersburg-Clearwater. *West Palm Beach-Boca Raton ...
The virus exits the host cell by lysis, and viroporins. Horses serve as the natural host. ERBV's appear to infect most foals ... Vero), equine foetal kidney (EFK), and is able to infect humans. The RNA genome of the virion is inside the capsid that is ... The virion essentially is a nucleocapsid that is visible under an electron microscope and is able to infect cultured cells from ... Entry into the host cell is achieved by attachment of the virus to host receptors, which mediates endocytosis. Replication ...
Furthermore, plant extracts of water spinach inhibit cancer cell growth of Vero, Hep-2 and A-549 cells, though have moderate ...
Cell. 150 (3): 457-469. doi:10.1016/j.cell.2012.07.009. ISSN 0092-8674. PMC 3426505. PMID 22840920. Hsieh, PingHsun; Woerner, ... Vero tapir (Tapirus veroensis) (H) Mixotoxodon (H) Several Felidae spp. Saber-toothed cat (Smilodon fatalis) (C) Scimitar cat ( ...
Manufacturing of vaccine for the Phase III trial was done by Merck, using the Vero cell line, which Merck already used to make ... Knowledge and expertise including but not limited to purification techniques, cell lines, materials, software codes and their ...
VERO and NIH3T3 cell lines compared with the other cell lines. GRCh38: Ensembl release 89: ENSG00000139292 - Ensembl, May 2017 ... Paneth cells, enteroendocrine cells, goblet cells, tuft cells, columnar cells and the M cells over an adult's entire lifetime. ... ve stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro". Cell Stem Cell. 6 (1): 25-36. doi ... ve stem cells reside in the isthmus, the region between the pit cells and gland cells, where most cellular proliferation takes ...
Shaw, Vero (1878-1881). The Illustrated Book Of The Dog. London. Shaw, Vero (1878-1881). The Illustrated Book Of The Dog. ... Cell Reports. 19 (4): 697-708. doi:10.1016/j.celrep.2017.03.079. PMC 5492993. PMID 28445722. Almirall, Leon V. (1941). Canines ... Cell Reports. 19 (4): 697-708. doi:10.1016/j.celrep.2017.03.079. PMC 5492993. PMID 28445722. Refer Figure 4 and Supplementary ...
Vero cells are a lineage of cells used in cell cultures. The Vero lineage was isolated from kidney epithelial cells extracted ... Vero E6, also known as Vero C1008 (ATCC No. CRL-1586) This line is a clone from Vero 76. Vero E6 cells show some contact ... F6B1.1) HeLa cells Immortalised cell line History and Characterization of the Vero Cell Line -- A Report prepared by CDR ... The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus. Vero cells are used for ...
... Rose-may Delrue Rose-may.Delrue at fundp.ac.be Fri Aug 29 08:06:58 EST 1997 *Previous message ... Does anyone has ever heard of macropinocytosis events or macropinocytosis stimulators usefull with Vero cells? Thanxs Rose-may ...
... and 36 after dose 1 of a 2-dose primary series of inactivated Vero cell culture--derived JE vaccine (JE-VC [manufactured as ... The updated recommendations included information on use of a new inactivated, Vero cell culture--derived JE vaccine (JE-VC [ ... Effect of pre-existing anti-tick-borne encephalitis virus immunity on neutralising antibody response to the Vero cell-derived, ... Long-term immunogenicity of the new Vero cell-derived, inactivated Japanese encephalitis virus vaccine IC51: six and 12 month ...
Vero cell cultivation for vaccine production. Anchorage-dependent cells, such as Vero cells, are widely used as a platform for ... To cultivate Vero cells in stirred-tank bioreactors, they require a growth matrix to attach to. The development of processes at ... HomeService & SupportEppendorf Training-CenterWebinarsRecorded WebinarsRecorded webinar: Vero cell cultivation for vaccine ... We demonstrate the great potential of using packed-bed bioreactors or microcarrier spin filter for attachment cell-based ...
load in Vero cells was performed using CT values for each sample (culture of Vero cells and Rickettsia, 1, 2, 24, 48 and 72 h ... in Vero cells and uninfected Vero cells (Control) stained according to the Giménez method (Giménez 1964) (1000× magnification, ... Rickettsia-like structures were observed in Giménez-stained Vero cells (Fig. 1A). Considering viable Vero cells (based on ... ß-actin qPCR analysis over a 72 h time course of infection in Vero cells. It was evident that the amount of Vero cell-infecting ...
MTT assay was performed to assess viability of the normal kidney Vero cells treated with CDDP and/or AEC. Cells were stained ... AEC did not alter activity of CDDP-induced cytotoxicity of breast and liver cancer cells. AEC treatment of Vero cells prevented ... CDDP-treated Vero cells exhibited hallmarks of apoptosis; these hallmarks were significantly suppressed in the presence of AEC ... The aqueous extract of cinnamon bark ameliorated cisplatin-induced cytotoxicity in vero cells without compromising the ...
A Booster Dose of Inactivated Vaccine (Vero Cell) Against EV71 in Chinese Children. This study has been completed. ... The phase II study of inactivated vaccine (vero cell) against EV71 has completed last month in Jiangsu Province in China. The ... A Booster Dose of Inactivated Vaccine (Vero Cell) Against EV71 in Chinese Children. ...
The clearance of host cell DNA is a critical indicator for Vero-cell culture-derived rabies vaccine. In this study, we ... Removing residual DNA from Vero-cell culture-derived human rabies vaccine by using nuclease.. [Si-Ming Li, Fu-Liang Bai, Wen- ... Dot-blot hybridization analysis showed that the residual host cell DNA was 5.0xa0IU/ml. Immunogenicity test and protection ... evaluated the clearance of DNA in Vero-cell culture-derived rabies vaccine by purification process utilizing ultrafiltration, ...
Inhibitory concentration required to produce cytotoxicity against VERO cells; Not determined. ...
Elements of the cytoskeletal and microtubule networks were imaged in the Vero cell culture illustrated in this section by ... treating the fixed and permeabilized cells with mouse anti-alpha-tubulin primary antibodies, followed by goat anti-mouse ... Normal African Green Monkey Kidney Epithelial Cells (Vero). The production of antibodies in vitro was introduced by Georges K ... Ultimately a cell line is established from a single cell (monoclonal) in which all of the cells produce an identical antibody. ...
Immunogenicity and Safety of Inactivated Vero Cell Derived Japanese Encephalitis Vaccine in Thai Children (JE0153). This study ... This study aims to evaluate the immunogenicity and safety of inactivated Vero cell derived JE vaccine (Beijing P-3 strain) ... Immunogenicity and Safety of Inactivated Vero Cell Derived Japanese Encephalitis Vaccine in Thai Children. ...
... the adherent culture of normal African green monkey kidney cells illustrated in this section was treated with a cocktail of ... View a smaller image of the African green monkey kidney epithelial (Vero) cells. ... Normal African Green Monkey Kidney Epithelial Cells (Vero). An important membrane polypeptide in peroxisomes, the peroxisomal ... In a double immunofluorescence labeling experiment, the adherent culture of normal African green monkey kidney cells ...
As one of the WHO-recommended cell lines for producing influenza vaccines, Vero cells do not efficiently support the growth of ... A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells.. [Hong Zhang, Qinglin Han, Xianqiang Ping ... a single mutation of K86R in the NS2 protein can sufficiently render the high-yielding property to the PR8 virus in Vero cells ... virus could serve as a promising vaccine donor strain in Vero cells. ...
... interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells ... Collectively, we demonstrated that annexin II is one of the host factor involved in DENV2 binding on Vero cells. ... interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells ... Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells Isah Abubakar Aliyu 1,2. ...
Additional studies showed that heat does not affect the stability of AFB1 in milk and that the Vero cell model can be used to ... in Vero cells by two independent assays: the green fluorescent protein (GFP) assay, as a measure of protein synthesis by the ... cells, and the microculture tetrazolium (MTT) assay, as a measure of cell viability. The results demonstrate a non-linear dose- ... Vero cells; bioactivity; toxicity; non-linear dose effect; milk; meat; MTT assay; GFP assay; food safety aflatoxin B1; Vero ...
A Phase II Clinical Trial for Inactivated Vaccine (Vero Cell) Against EV71 in Chinese Children and Infants. The safety and ... inactivated vaccine(vero cell) against EV71 of (without adjuvant) 640U /0.5ml in 120 children aged 12-36 months old on day0,28 ... inactivated vaccine(vero cell) against EV71 of (without adjuvant) 640U /0.5ml in 120 infants aged 6-11 months old on day0,28 ... inactivated vaccine(vero cell) against EV71 of 160U /0.5ml in 120 children aged 12-36 months old on day0,28 ...
We have recently succeeded in propagating a third strain from a urogenital specimen from a patient with urethritis in Vero cell ... of the examined Vero cells. Various stages of penetration into the cells could be observed. The flask-shaped organisms seemed ... Intracellular location of Mycoplasma genitalium in cultured Vero cells as demonstrated by electron microscopy Int J Exp Pathol ... We have recently succeeded in propagating a third strain from a urogenital specimen from a patient with urethritis in Vero cell ...
We describe a method to process and screen field-collected mosquitoes for a diversity of viruses by Vero cell culture assay. By ... 3. Preparation of Vero cells *Decant culture media from one large tissue culture flask (175 cm2) of confluent Vero cells (Clone ... Place tubes back into freezer racks until inoculated into Vero cells.. 5. Inoculation of Vero cells with mosquito pool ... Wash Vero cells by adding a 5 mL aliquot of PBS and swirl over layer of cells and along sides of flask making sure to ...
Findings: All transfected cells in our study demonstrate no noticeable differences within transfected-Vero cell cultures with ... Vero cell lines expressing nuclear location signals of Penaeus merguiensis hepandensovirus: An early study. ... Densovirinae are intranuclear and require S-cells in their S-phase for all or most of all their replication and assembly. ... In the future, the study of virus-host interaction using cell cultures as models remains a major challenge.. ...
... um método para processar e tela de campo coletados mosquitos para uma diversidade de vírus por cultura de células Vero... ... 3. Preparação de células Vero * Decantar meios de cultura de um grande frasco de cultura de tecidos (175 cm 2) de células Vero ... Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay. J. Vis. Exp. (52), e2889, doi:10.3791 ... Lave as células Vero, adicionando uma alíquota de 5 mL de PBS e agite sobre a camada de células e ao longo dos lados do frasco ...
... was recovered in Vero cells. A single capsid protein mutation (S66L) was important for Vero cell-adaptation. Mutations were ... In this study, 10 genetically distinct Vero cell-adapted JEV SA14-14-2 variants were isolated and a recombinant wild-type JEV ... also identified that modulated virus sensitivity to type I interferon-stimulation in Vero cells. A subset of JEV SA14-14-2 ... Past attempts to adapt this virus to replicate in cells that are more favorable for vaccine production resulted in mutations ...
Over 1 month, variability of the assay using a partially purified Vero cell extract was between 8 and 17%, and accuracy was ... could therefore be used in the near future to quantify dengue virus type 4 during production and purification from Vero cells ... Over 1 month, variability of the assay using a partially purified Vero cell extract was between 8 and 17%, and accuracy was ... Absolute quantification of dengue virus serotype 4 chimera vaccine candidate in Vero cell culture by targeted mass spectrometry ...
Evaluated in vitro for antiviral activity in vero cell cultures against Coxsackie virus type B4. ...
Dynamics of Actin-Based Movement byRickettsia rickettsii in Vero Cells. Robert A. Heinzen, Scott S. Grieshaber, Levi S. Van ... Dynamics of Actin-Based Movement byRickettsia rickettsii in Vero Cells. Robert A. Heinzen, Scott S. Grieshaber, Levi S. Van ... Dynamics of Actin-Based Movement byRickettsia rickettsii in Vero Cells. Robert A. Heinzen, Scott S. Grieshaber, Levi S. Van ... Dynamics of Actin-Based Movement byRickettsia rickettsii in Vero Cells Message Subject (Your Name) has forwarded a page to you ...
Possible involvement of receptors in the entry of Kunjin virus into Vero cells NG M. L. ... The cytotoxic action of diphtheria toxin and its degradation in intact Vero cells are inhibited by bafilomycin A1, a specific ... Japanese Encephalitis Virus Infection in Vero Cells:The Involvement of Intracellular Acidic Vesicles in the Early Phase of ... Low pH-induced cell fusion in flavivirus-infected Aedes albopictus cell cultures. RANDOLPH V. B. ...
... authors tested benchtop single-use and glass bioreactors with Fibra-Cel disks would be suitable for cultivation of Vero cells. ... Vero cells are anchorage-dependent cells that are used widely as a platform for viral vaccine production. In stirred-tank ... Vero cells are anchorage-dependent cells that are used widely as a platform for viral vaccine production (1). In stirred-tank ... However, such disks have not been tested for the cultivation of Vero cells. We tested whether benchtop single-use and glass ...
At the same time, both viral replication and host cells viability were lowered. Viral replication, Vero cell viability, p38 ... In summary, our results demonstrated that HSV-1 infection in Vero cells induced an elevation of p38 MAPK and Bcl-2. TCS ... Results showed that HSV-1 infection induced an elevation of phosphorylated p38 and Bcl-2 in Vero cells, which could be ... Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells ...
Designed as a VERO expansion and virus production media specifically optimized for VERO-based virus production in both 2D and ... OptiVERO - Virus Production Using VERO Cells in Flasks without Serum in Chemically Defined Cell Culture Media Application Note ... OptiVERO - Virus Production Using VERO Cells with Microcarriers Without Serum in Chemically Defined Cell Culture Media ... OptiVERO® was specifically optimized for VERO-based virus production in both 2D and 3D culture formats. This media formulation ...
Cheap Unlocked Cell Phones Sanyo Vero Basic Bluetooth Camera Blue Flip Phone Sprint - Refurbished Condition - Communicate with ... your peers and get turn-by-turn directions to new locations, using the GPS technology of the Sanyo Vero phone. Click those ... Sanyo Vero Basic Bluetooth Camera Blue Flip Phone Sprint. Regular Price: $64.99 ... Sanyo Vero Basic Bluetooth Camera Blue Flip Phone Sprint. .accessories_for_your_phone,#lowstock { margin-bottom: 5px; margin- ...
Safety and immunogenicity of two different doses of a Vero cell-derived, whole virus clade 2 H5N1 (A/Indonesia/05/2005) ... H5N1 Vero cell-derived influenza vaccine in healthy adults (21-45 years) from a region where the virus has been circulating ( ...
  • F6B1.1) HeLa cells Immortalised cell line History and Characterization of the Vero Cell Line -- A Report prepared by CDR Rebecca Sheets, Ph.D., USPHS CBER/OVRR/DVRPA/VVB for the Vaccines and Related Biological Products Advisory Committee Meeting to be held on May 12, 2000 OPEN SESSION www.fda.gov pdf Yasumura Y, Kawakita M (1963). (wikipedia.org)
  • Vaccination is the most effective way to prevent and control infection by influenza viruses, and a cell-culture-based vaccine production system is preferred as the future choice for the large-scale production of influenza vaccines. (sigmaaldrich.com)
  • As one of the WHO-recommended cell lines for producing influenza vaccines, Vero cells do not efficiently support the growth of the current influenza A virus vaccine donor strain, the A/Puerto Rico/8/1934 (PR8) virus. (sigmaaldrich.com)
  • The use of cell-culture technologies for the manufacture of influenza vaccines might contribute to improved strain selection and robust vaccine supplies. (flu.org.cn)
  • Kathmandu [Nepal], July 2 (ANI): Nepal will procure four million doses of Vero Cell vaccines from China under a non-disclosure agreement signed between the two nations, sources said though officials from the Ministry of Health and Population declined to comment on the issue. (aninews.in)
  • This cell development platform has proven effective in the creation of a number of viral vaccines. (vxpbiologics.com)
  • The following cases demonstrate the effectiveness of the Vero platform in enabling the rapid development of new vaccines. (vxpbiologics.com)
  • The Vero platform has proven effective in rapidly developing novel influenza vaccines. (vxpbiologics.com)
  • Prior to the H1N1 outbreak in 2009, the Vero platform had already been used to derive an inactivated H5N1 vaccine, which requires no adjuvants (irritating chemicals that boost immune response, which are required by some vaccines). (vxpbiologics.com)
  • Viral diseases like rabies, rotavirus and influenza are causing many deaths worldwide, resulting in a strong demand for more productive manufacturing techniques for vaccines, including those based on scalable bioreactor cell culture systems. (biopharma-reporter.com)
  • The benefits of using cell culture for vaccine production to meet pandemic demands are well documented and the reason behind huge financial investment, cell culture can and is being used to produce a wide range of vaccines and exhibiting even more benefits. (vaccinenation.org)
  • Dr Neuza Maria Frazatti Gallina, Coordinator Projetos de Desenvolvimento e Produção de Vacinas Virais at Instituto Butantan in Brazil, joined us at the World Vaccine Cell Culture Congress 2012 in Washington to present on mammalian cell sustainability, in relation to Rabies, Rotavirus and Dengue vaccines in vero cell. (vaccinenation.org)
  • These cell lines can then be used to produce viral vaccines in vero cell, such as for Rabies, Rotavirus and Dengue vaccines. (vaccinenation.org)
  • Although mouse brain-derived, inactivated Japanese encephalitis vaccines (JE-MBs) have been successfully used for a long time, potential rare neurological complications have prompted the development of a Vero cell culture-derived inactivated vaccine (JE-VC). (biomedcentral.com)
  • Hardly any Americans know that there are infected African green monkey kidney cells in the smallpox and DTap vaccines , but all the M.D.s know, but the CDC doesn't like when patients ask questions. (naturalnews.com)
  • The Vero cell line is susceptible to multiple viruses and has widely contributed to the creation of vaccines for human use. (altogen.com)
  • 200,000 doses of Vero Cell vaccines were donated by the Tibet Autonomous Region Government, PRC. (nepalnews.com.np)
  • New York City, NY: January 14,2020, Published via (Wired Release) Global Vero Cell Media Market in-depth review report holds the description of all the essential focuses concerning the Vero Cell Media market. (5gigs.com)
  • The report additionally explored the global Vero Cell Media market development pattern based on regional order. (5gigs.com)
  • The Vero Cell Media market report on trends and improvements focuses on markets and materials, capacities, technologies, CAPEX cycle and the changing structure of the Global Vero Cell Media Market. (5gigs.com)
  • New York City, NY: November 16, 2019 - Published via (Wired Release) - The report on Global Vero Cell Media Market delivers recent industry information and highlights several trends impacting the growth of the market. (ahealthnewstoday.com)
  • The aim of the Global Vero Cell Media Market research report is to describe the crucial segment and competition in Pharmaceuticals and Healthcare industry. (ahealthnewstoday.com)
  • The report comprehensively analyzes the Global Vero Cell Media Market status, supply, sales, and production. (ahealthnewstoday.com)
  • On the whole, the report covers the Global Vero Cell Media Market view and its growth probability for upcoming years. (ahealthnewstoday.com)
  • To develop a better understanding of the significance of low dose effects, in the present study, we developed quantitative methods for the detection of biologically active aflatoxin B 1 (AFB1) in Vero cells by two independent assays: the green fluorescent protein (GFP) assay, as a measure of protein synthesis by the cells, and the microculture tetrazolium (MTT) assay, as a measure of cell viability. (mdpi.com)
  • The cytotoxic and anti-genotoxic activities were evaluated against Vero cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and comet assays. (deepdyve.com)
  • Competition between CRM9 and diphtheria toxin for cell membrane receptors could .not be demonstrated directly in Vero cell cytotoxicity assays however, CRM197 afforded complete protection to Vero cells from intoxication by both CRM9 and diphtheria toxin. (dtic.mil)
  • The capacity to express active Stx1 and Stx2 toxins was determined in Vero cell cultures and the concentration of Stx was evaluated by 50% lethal dose (LD 50 ) assays, observing inhibition of damaged cells by specific monoclonal antibodies. (frontiersin.org)
  • ATCC offers well-characterized cells that can be used to refine traditional cell-based experiments or construct high-throughput assays, reducing the need for in vivo studies. (atcc.org)
  • Physiologically-relevant cell-based assays are critical for the biomedical and toxicity data that cannot be obtained from animal models or humans. (atcc.org)
  • The cultured cells were incubated with different concentrations of cobalt chloride ranging from 0.5 to 1,000 mu M, and cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and resazurin assays. (hacettepe.edu.tr)
  • Research strains transfected with viral genes: Vero F6 is a cell transfected with the gene encoding HHV-1 entry protein glycoprotein-H (gH). (wikipedia.org)
  • Anchorage-dependent cells, such as Vero cells, are widely used as a platform for viral vector production. (eppendorf.com)
  • Additional DNA samples from Vero cells infected with R. parkeri , R. rhipicephali and Rickettsia amblyommii were obtained using the extraction method described previously, using a QIAamp DNA Blood Mini Kit (Qiagen®) and a High Pure Viral Nucleic Acid Kit (Roche Applied Science®), to test quality by qPCR. (scielo.br)
  • At the same time, both viral replication and host cells viability were lowered. (ovid.com)
  • Viral replication, Vero cell viability, p38 MAPK and Bcl-2 were further reduced with the addition of a p38 MAPK inhibitor (SB203580). (ovid.com)
  • The second stage of the research involved the use of immunofluorescent staining to locate viral proteins within infected cells in order to inform future process optimization. (uwaterloo.ca)
  • The persistently infected cell supernatant preparations shared several features with DI particle preparations from other viral systems, such as their amplification to detectable levels after two to four passages of virus. (microbiologyresearch.org)
  • Electron microscopy image of Vero E6 cells infected with SARS-CoV-2 virus, the causative agent of COVID-19, produce filopodia protrusions (finger-like projections) extending out from the cell surface to enable budding of viral particles (circular spheres) and infection of nearby cells. (globenewswire.com)
  • Here we investigated the presence of viruses in 12 pools of sand flies captured in three climatic periods in RAPELD grids at Rio Claro, Chapada dos Guimarães and at Pirizal, North Pantanal, Mato Grosso State, Midwestern Brazil by HTS, viral isolation of a putative Phlebovirus positive pool in Vero cells, RT-PCR and transmission electron microscopy (TEM). (springer.com)
  • mean ±S.D.). C) Viral protein synthesis was analyzed in infected cells (MOI 1) at 16 hpi in the presence of IPA-3 at the indicated concentrations. (nih.gov)
  • Then, cells were infected (MOI 1) for 16 h and viral protein synthesis was analyzed by immunoblotting with an anti-ASFV antibody. (nih.gov)
  • Last, overexpressing β 4 in undifferentiated and differentiated H9c2 cells reduced DENV infection and decreased the abundance of the viral proteins NS1, NS3, and E-protein. (sciencemag.org)
  • In the absence of viral infection, Ser221 in the N-terminal kinase domain was phosphorylated constitutively, whereas both Thr573 in the C-terminal kinase domain and Ser380 between the two kinase domains were not phosphorylated in confluent cells. (qxmd.com)
  • The utilization of Vero cells for vaccine production has contributed significantly to controlling viral diseases and improving the healthcare system in the US and around the world. (wpi.edu)
  • Vero E6 cells show some contact inhibition, so are suitable for propagating viruses that replicate slowly. (wikipedia.org)
  • We investigated the safety, immunogenicity, and protective efficacy of a Vero-cell-culture-derived influenza vaccine, and assessed the correlation between vaccine efficacy and haemagglutination inhibition antibody titre. (flu.org.cn)
  • Inhibition by Agaricus blazei Murill fractions of cytopathic effect induced by western equine encephalitis (WEE) virus on VERO cells in vitro. (semanticscholar.org)
  • To several viruses which have cytopathic effects (CPE) on VERO cells, inhibition of these effects by the ethanol fractions was tested. (semanticscholar.org)
  • VERO C1008 exhibits some degree of contact inhibition after forming a monolayer and is therefore useful in growing slow replicating viruses. (bioz.com)
  • The inhibition of protein synthesis in Vero cells by CRM9 and wild-type toxin proceeded at the same rate when equally toxic doses of each protein were compared. (dtic.mil)
  • Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells. (qxmd.com)
  • Removing residual DNA from Vero-cell culture-derived human rabies vaccine by using nuclease. (sigmaaldrich.com)
  • The clearance of host cell DNA is a critical indicator for Vero-cell culture-derived rabies vaccine. (sigmaaldrich.com)
  • In this study, we evaluated the clearance of DNA in Vero-cell culture-derived rabies vaccine by purification process utilizing ultrafiltration, nuclease digestion, and gel filtration chromatography. (sigmaaldrich.com)
  • Our study on the Europe Vero Cell Rabies Vaccine Industry Competitive market is driven by the adoption of an exhaustive approach towards the industry analysis comprising of the overall market size and revenue growth, recent market developments, growth drivers, market segmentation, opportunities and challenges that are influencing the growth of the market. (crifax.com)
  • The Europe Vero Cell Rabies Vaccine Industry Competitive market is estimated to attain notable absolute $ opportunity value in 2027 as compared to the value achieved in 2019. (crifax.com)
  • The study of the Europe Vero Cell Rabies Vaccine Industry Competitive market offers a detailed analysis including a risk analysis that covers demand and supply risk affecting the growth of the market accompanied by existing and future market trends prevalent in the region. (crifax.com)
  • Densovirinae are intranuclear and require S-cells in their S-phase for all or most of all their replication and assembly. (omicsonline.org)
  • Rose bengal inhibits herpes simplex virus replication in vero and human corneal epithelial cells in vitro. (arvojournals.org)
  • To determine the binding properties of rose bengal, the authors compared binding of the dye to HSV-1-infected and uninfected cells, determined the cellular binding sites of the dye, and investigated the effects of rose bengal on HSV-1 replication in dye-treated cells in vitro. (arvojournals.org)
  • Pretreatment of Vero and HCEC with different concentrations of rose bengal and exposure to 148 microW/cm2 of white light for 2 min reduced the ability of both cell types to support HSV-1 replication. (arvojournals.org)
  • Vero cells, in the absence of rose bengal, supported HSV-1 replication, whereas pre-treatment with 0.05% rose bengal reduced the yield of HSV-1 by 99.99% (P less than 0.000001) and 1% rose bengal completely prevented HSV replication. (arvojournals.org)
  • in many aspects such as virion ultrastructure in which the circular and oval forms were most commonly observed, mode of virus replication in infected cells, and the sequential development of cytopathic effects. (virosin.org)
  • In cells overexpressing the β 4 subunit, dengue virus replication was inhibited. (sciencemag.org)
  • In this study, TTSPs including TMPRSS2, HAT, DESC1, and MSPL were tested for their ability to facilitate PEDV replication in Vero cells. (duhnnae.com)
  • Moreover, the use of Vero cells expressing TMPRSS2 and MSPL facilitated PEDV replication in the absence of exogenous trypsin. (duhnnae.com)
  • Bhaktapur [Nepal], June 9 (ANI): Stalled for several months due to shortage of COVID-19 vaccine, the inoculation drive in Nepal resumed on Tuesday after it received one million shots of China-made Vero Cell last week. (aninews.in)
  • Kathmandu [Nepal], April 7 (ANI): Long queues were seen on Wednesday at the vaccine booth in Sukraraj Tropical and Infectious Disease Hospital in Kathmandu as Nepal started the inoculation drive with China-made COVID-19 vaccine Vero Cell, amid safety concerns. (aninews.in)
  • Kuala Lumpur - Mesyuarat Pihak Berkuasa Kawalan Dadah (PBKD) ke-356 hari ini bersetuju meluluskan pendaftaran secara bersyarat bagi produk vaksin COVID-19 'CoronaVac Suspension for Injection COVID-19 Vaccine (Vero Cell), Inactivated' untuk kegunaan semasa bencana. (kualalumpurpost.net)
  • EMA's human medicines committee (CHMP) has started a rolling review of COVID-19 Vaccine (Vero Cell) Inactivated, developed by Sinovac Life Sciences Co., Ltd. The EU applicant for this medicine is Life'On S.r.l. (pecssun.hu)
  • EMA will assess the compliance of COVID-19 Vaccine (Vero Cell) Inactivated with the usual EU standards for effectiveness, safety and quality. (pecssun.hu)
  • COVID-19 Vaccine (Vero Cell) Inactivated is expected to prepare the body to defend itself against infection with SARS-CoV-2. (pecssun.hu)
  • COVID-19 Vaccine (Vero Cell) Inactivated also contains an 'adjuvant', a substance that helps strengthen the immune response to the vaccine. (pecssun.hu)
  • Vero cells are a lineage of cells used in cell cultures. (wikipedia.org)
  • The production of antibodies in vitro was introduced by Georges K hler and C sar Milstein, who first employed cultures of fused cells to isolate antibodies of defined specificity. (fsu.edu)
  • We have recently succeeded in propagating a third strain from a urogenital specimen from a patient with urethritis in Vero cell cultures. (nih.gov)
  • Findings: All transfected cells in our study demonstrate no noticeable differences within transfected-Vero cell cultures with desired NLSs genes. (omicsonline.org)
  • In the future, the study of virus-host interaction using cell cultures as models remains a major challenge. (omicsonline.org)
  • Low pH-induced cell fusion in flavivirus-infected Aedes albopictus cell cultures. (nii.ac.jp)
  • Until viable options of suspension cell culture are available, Vero cell cultures still rely on adherent bioprocesses and have thus been developed in this work. (physiciansweekly.com)
  • A lineage of cells used in cell cultures and isolated from kidney epithelial cells of the African green monkey ( Cercopithecus aethiops ). (thefreedictionary.com)
  • 66 strains were isolated from children who live in Mexico City, and Stx effects were evaluated in Vero cell cultures. (frontiersin.org)
  • From HYPER Stack ® cell culture vessels to CellSTACK® culture chambers to microcarriers, Corning provides the full range of tools you'll need to successfully perform and improve adherent-based cultures for vaccine scale-up and production. (corning.com)
  • This study shows how these microcarriers enable improved optimization and culturing of adherent cells in dynamic cultures. (corning.com)
  • This study aims to evaluate the immunogenicity and safety of inactivated Vero cell derived JE vaccine (Beijing P-3 strain) produced by Liaoning Cheng Da Biotechnology Co., Ltd, China 'JEVAC' in Thai children. (clinicaltrials.gov)
  • Past attempts to adapt this virus to replicate in cells that are more favorable for vaccine production resulted in mutations that significantly reduced immunogenicity. (ajtmh.org)
  • Safety and immunogenicity of two different doses of a Vero cell-derived, whole virus clade 2 H5N1 (A/Indonesia/05/2005) influenza vaccine. (diva-portal.org)
  • An open-label, randomized, phase I/II study therefore assessed the immunogenicity and safety of two different dose levels of an inactivated, non-adjuvanted, whole virus clade 2.1 (A/Indonesia/05/2005) H5N1 Vero cell-derived influenza vaccine in healthy adults (21-45 years) from a region where the virus has been circulating (Hong Kong) as well as Singapore. (diva-portal.org)
  • In vitro cytotoxicity evaluation demonstrated that seed extract of C. surattensis was non-cytotoxic against the Vero cells. (deepdyve.com)
  • A preliminary characterization of these lysogenic strains identified a new class of CRMS which were antigenically and enzymatically Indistinguishable from wild-type toxin but were considerably less cytotoxic for Vero cells. (dtic.mil)
  • Bead enzyme-linked immunosorbent assay and Vero cell assay were performed to detect and evaluate the cytotoxic effect of the Shiga toxins produced by the strains. (cdc.gov)
  • The aim of this study was to investigate the possible time- and dose-dependent cytotoxic effects of cobalt chloride on Vero cells. (hacettepe.edu.tr)
  • CoCl2 had dose- and time-dependent cytotoxic effects in Vero cells. (hacettepe.edu.tr)
  • In a double immunofluorescence labeling experiment, the adherent culture of normal African green monkey kidney cells illustrated above was treated with a cocktail of mouse anti-histones (pan) and rabbit anti-PMP 70 (peroxisomal membrane protein) primary antibodies, followed by goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa Fluor 568 and Alexa Fluor 488, respectively, to target the nuclear histone proteins and peroxisomes. (fsu.edu)
  • The live-attenuated Japanese encephalitis virus (JEV) SA14-14-2 vaccine, produced in primary hamster kidney cells, is safe and effective. (ajtmh.org)
  • I. Propagation and plaque assay of Tacaribe virus in a line of African green monkey kidney cells (Vero). (addexbio.com)
  • The Vero epithelial cell line is taken from kidney cells of an African green monkey (Cercopithecus aethiops). (altogen.com)
  • A bottleneck exists in the production process which is hypothesized to be the result of virus particles being directed towards both cell-cell and cell-substrate (adherent surface) junctions. (uwaterloo.ca)
  • Our single-use adherent and suspension cell culture products provide reliable, predictable scale-up in bioprocess and cell therapy. (corning.com)
  • In the United States, the adherent cell line Vero 76 has never been grown within a packed-bed bioreactor using Eppendorf's BioFlo 320 system, however they are typically grown on microcarrier beads in a pitched-blade vessel. (wpi.edu)
  • The present study aims to determine if of the three putative NLSs of PmeHDV are functioning by transfecting NLS-inserted-plasmid DNAs into mammalian cell culture (Vero) using a transfection reagent. (omicsonline.org)
  • In brief, cryogenic tubes containing Rickettsia -infected Vero cells were rapidly thawed, and their contents were added to flasks with an uninfected Vero cell monolayer, and incubated at 28ºC without CO 2 . (scielo.br)
  • This weekend, in preparation for the experiment, we also had to seed our flasks with Vero cells. (caseynparker.com)
  • Here, we conducted electron microscopy studies on Vero cells infected with PPRV strain Nigeria 75/1 (obtained from the virus bank of the China Institute of Veterinary Drug Control) that were seeded on 75-cm 3 tissue culture flasks. (virosin.org)
  • Because obtaining glycosylated product in large amounts on culture dishes is tedious, the efficient mass production of Gb3 analogue was accomplished using Vero cells cultured on microcarriers in spinner flasks. (biomedcentral.com)
  • The 'Vero' lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp. (wikipedia.org)
  • In this procedure, termed monoclonal antibody production, antibodies are first produced in mice, and activated B-lymphocytes from the host's spleen (the source of antibodies) are fused with cultured plasma tumor myeloma cells (that do not produce antibodies) from mice of the same strain. (fsu.edu)
  • Each dose of JEVAC contains 0.5 mL. of inactivated Vero cell derived JE vaccine (Beijing P-3 strain). (clinicaltrials.gov)
  • Because the NS2(K86R) mutation does not increase PR8 virulence in either mice or embryonated eggs, the PR8-NS2(K86R) virus could serve as a promising vaccine donor strain in Vero cells. (sigmaaldrich.com)
  • The "ACAM529" strain is subject to two gene deletions and requires a complementary African Green Monkey Kidney (Vero) cell line (AV529-19) to replicate. (uwaterloo.ca)
  • Murray Valley encephalitis (MVE) virus strain OR2 was serially passaged on Vero cells to establish a persistent infection which was maintained for over 300 days. (microbiologyresearch.org)
  • A) Indirect immunofluorescence detection of MRV in Vero E6 cells infected with strain B/03. (bioz.com)
  • When the H1N1 pandemic struck the US, this Vero-developed vaccine was used to streamline the development, manufacture and licensing of a novel vaccine that was effective against the new strain. (vxpbiologics.com)
  • Here we used the ASFV virulent isolate Ba71, adapted to grow in Vero cells (Ba71V), and the virulent strain E70 to demonstrate that entry and internalization of ASFV includes most of the features of macropinocytosis. (nih.gov)
  • A Vero cell attenuated porcine epidemic diarrhea virus (PEDV) strain, DR13, was distinguished from wild-type PEDV using restriction enzyme fragment length polymorphism (RFLP). (snu.ac.kr)
  • b ) Infectivity of rLASV-GFP in A549, HeLa, Huh7, and Vero E6 cells at the indicated MOIs at 24, 48, and 72h PI as determined by GFP-expression using high-content imaging. (bioz.com)
  • Cytopathic effects of shigellae on Vero & HeLa cells. (bvsalud.org)
  • We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. (mdpi.com)
  • Chee, H.-Y. Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells. (mdpi.com)
  • Dengue non-structural protein 5 polymerase complexes with promyelocytic leukemia protein (PML) isoforms III and IV to disrupt PML-nuclear bodies in infected cells. (nature.com)
  • found that β 4 abundance and nuclear localization were increased in a cardiac cell line in response to treatment with type I interferon (IFN) or infection with dengue virus. (sciencemag.org)
  • β 4 subunit abundance was increased, and it translocated to the nucleus, in cells treated with IFN-β, infected with dengue virus (DENV), or transfected with poly(I:C), a synthetic analog of double-stranded RNA. (sciencemag.org)
  • Unique from mammalian cells, the Vero cell line is interferon-deficient since it does not produce type 1 interferons after being infected by viruses. (altogen.com)
  • The report also presents forecasts for Vero Cell Media investments from 2017 till 2022. (newzy.net)
  • A time-lapse video of moving intracellular bacteria was obtained by laser-scanning confocal microscopy of infected Vero cells synthesizing β-actin coupled to green fluorescent protein (GFP). (asm.org)
  • In the present work, we have examined through immunofluorescence and confocal microscopy, the subcellular localization of PAR in an epithelial monkey kidney cell line (VERO). (mysciencework.com)
  • The Vero cell platform vaccine production technology addresses precisely this need. (vxpbiologics.com)
  • At the end of 2014, Baxter sold its vaccine production technology based on Vero cell culture to Nanotherapeutics. (wikipedia.org)
  • However, such disks have not been tested for the cultivation of Vero cells. (bioprocessintl.com)
  • We tested whether benchtop single-use and glass bioreactors with a packed bed made of Fibra-Cel disks would be suitable for cultivation of Vero cells. (bioprocessintl.com)
  • Our results suggest that benchtop, packed-bed bioreactors filled with Fibra-Cel disks are well suited for the cultivation of the Vero cell line - and hence vaccine production - in small batch sizes. (bioprocessintl.com)
  • and samples of urine were assayed for arenavirus by cultivation in monolayers of Vero E6 cells (5). (thefreedictionary.com)
  • Rose bengal dye is thought to highlight corneal lesions induced by herpes simplex virus type 1 (HSV-1) by virtue of its binding to dead or dying HSV-1-infected epithelial cells. (arvojournals.org)
  • unlike normal mammalian cells, they do not secrete interferon alpha or beta when infected by viruses. (wikipedia.org)
  • Virus stocks were grown in Vero E6 cells and titrated by using a 50% tissue culture infectious dose ([TCID. (thefreedictionary.com)
  • As per the decision of the Government of Nepal, Ministry of Health and Population dated 2078/02/18 and District Disaster Management Center, Kathmandu dated 2078/02/23, the vaccination campaign against COVID-19, Chinese Vero Cell first dose campaign has been started from 2078/02/25 to fully comply with the public health standards. (nelomasi.com.np)
  • The first dose of Vero Cell Vaccine will be given to the people for the age group of 60 to 64 years from 10:00 AM to 4:00 PM from 2078/02/25. (nelomasi.com.np)
  • Dose-dependent cytotoxicity was noted following exposure of cells to a parts per thousand yen250 mu M of CoCl2 for 24 h and a parts per thousand yen100 mu M concentrations of CoCl2 for 48-96 h. (hacettepe.edu.tr)
  • We demonstrate the great potential of using packed-bed bioreactors or microcarrier spin filter for attachment cell-based vaccine production. (eppendorf.com)
  • Serum-free production of rVSV-ZEBOV in Vero cells: microcarrier bioreactor versus scale-X™ hydro fixed-bed. (physiciansweekly.com)
  • The large-scale culture of Vero cells using a microcarrier culture method with repetitions produced about 30 mg of the Gb3 analogue. (biomedcentral.com)
  • The mass production of glycosides in Vero cells was carried out on a microcarrier with repeated administration of 12-azidododecyl lactoside. (biomedcentral.com)
  • The primary objective was the efficacy of the vaccine in preventing cell-culture-confirmed influenza infection with viruses that were antigenically matched to one of the vaccine strains. (flu.org.cn)
  • ATCC, your trusted source for cell lines, microbial strains, and other biological reagents, invites you to view one of the exciting webinars in our 2012 Excellence in Research series. (atcc.org)
  • Genomic DNA extraction from 100 µL of cells in suspension was performed using an Illustra RNAspin Mini RNA Isolation kit (GE Healthcare®) without RNase addition, according to the standard operating procedure (FUNED). (scielo.br)
  • Fundamental Techniques in Cell Culture: a Laboratory Handbook. (wikipedia.org)
  • The updated recommendations included information on use of a new inactivated, Vero cell culture--derived JE vaccine (JE-VC [manufactured as Ixiaro]) that was licensed in the United States in 2009. (cdc.gov)
  • The fusion product is a hybrid (termed a hybridoma ) of the two cells that continues to grow and divide in culture and produces large amounts of the antibody. (fsu.edu)
  • Unfused cells and non-productive fusions are eliminated by classical cloning techniques to purify the culture of antibody-producing cells. (fsu.edu)
  • A hybrid cell line produces only a single type of antibody, which can be used as a selection marker in serial dilution cloning by monitoring the culture medium for secreted antibody at each stage. (fsu.edu)
  • Elements of the cytoskeletal and microtubule networks were imaged in the Vero cell culture illustrated above by treating the fixed and permeabilized cells with mouse anti- alpha -tubulin primary antibodies, followed by goat anti-mouse secondary antibodies conjugated to Alexa Fluor 568. (fsu.edu)
  • We describe a method to process and screen field-collected mosquitoes for a diversity of viruses by Vero cell culture assay. (jove.com)
  • To monitor the culture, we quantified glucose and lactate concentrations in the medium daily and inferred from those parameters about cell growth. (bioprocessintl.com)
  • OptiVERO ® was specifically optimized for VERO-based virus production in both 2D and 3D culture formats. (invitria.com)
  • A pseudo co-culturing protocol was then developed using cell culture inserts. (uwaterloo.ca)
  • In a double-blind, placebo-controlled, phase 3 trial undertaken in 36 centres in the USA, healthy adults (aged 18-49 years) were randomly assigned in a 1:1 ratio to one injection of either placebo or Vero-cell-culture-derived influenza vaccine during the 2008-09 season. (flu.org.cn)
  • In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of normal African green monkey (Vero) cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (olympus-lifescience.com)
  • In contrast, as much as 10 μg/ml of BFA in culture medium did not affect either glycoprotein formation or production of infectious particles in C6/36 cells. (nus.edu.sg)
  • a: Negative staining of cell culture supernatant. (bioz.com)
  • Vero cell culture will allow detection of sorbitol fermenting STEC as well as O157. (health.gov.au)
  • However this method is slow and only available to laboratories with cell culture facilities. (health.gov.au)
  • Vero cells are a lineage of cells that are used in cell culture and these are cells that we will eventually infect with our virus. (caseynparker.com)
  • The cells that we distribute in the new flask are pulled from an existing cell culture. (caseynparker.com)
  • Decrease contamination risk, reduce labor and operating costs, and scale-up without increasing your footprint using Corning multilayer cell culture vessels. (corning.com)
  • Cryogenic preservation can minimize cell culture aging and evolution by stopping biological time, putting cells into true suspended animation, resulting in a more homogeneous cell population for biobanking research applications. (corning.com)
  • From proteins to antibodies to nucleic acid production, successfully scale-up your suspension culture for bioreactor seeding with Corning bioprocess and cell culture products. (corning.com)
  • After addition of BDM to the culture medium the area of cell contact with substratum gradually reduced - within 5 min of treatment cell lamellae became thicker, after 60 min the cell area decreased ∼ 70 %, and the cells became nearly rounded. (edu.kz)
  • Please feel free to comment and tell us your thoughts on the benefits of cell culture for vaccine production below! (vaccinenation.org)
  • The evolution of cell substrate to vaccine production has 4 distinct stages: Primary cell culture to Diploid cell lines to Continuous cell lines and finally, Stem cell lines. (vaccinenation.org)
  • In T. Noll ( Ed. ), Cells and Culture: Proceedings of the 20th ESACT Meeting (pp. 635-638). (mpg.de)
  • Our collection includes all the "classic" media formulations, and media specially formulated to optimize the growth of hybridoma, primary, or stem cells in culture. (atcc.org)
  • Gycosylated glycosides are released outside of cells to the surrounding culture medium. (biomedcentral.com)
  • Therefore, B16 cells take in amphipathic lactosides dissolved in the culture medium and sialylated them, before releasing them into the surrounding culture medium. (biomedcentral.com)
  • the elongated glycosides with C16 alkyl chains have been shown to remain mostly in the B16 cell, whilst those with C12 chains are released into culture medium [ 11 ]. (biomedcentral.com)
  • The production of the globotriaosyl ceramide (Gb3) analogue was optimized using appropriate conditions such as seeded cell number, concentration of the lactoside, time of incubation and culture medium volume. (biomedcentral.com)
  • View a larger image of the African green monkey kidney epithelial (Vero) cells. (fsu.edu)
  • View a smaller image of the African green monkey kidney epithelial (Vero) cells. (fsu.edu)
  • zeiss_microscopy: African green monkey kidney (Vero) cell. (uberflip.com)
  • By electron microscopy mycoplasmas were demonstrated intracellularly in about 10% of the examined Vero cells. (nih.gov)
  • To address these issues, serum-free production of rVSV-ZEBOV in Vero cells has been studied with the aim to test an alternative upstream process. (physiciansweekly.com)
  • A cell line taken from the kidneys of the African green monkey, Cercopithecus aethiops sabaeus, and used to grow certain viruses (e.g., poliovirus and influena A and B) for vaccine production. (thefreedictionary.com)
  • Production Analysis - Production of the Vero Cell Media is analysed with respect to different regions, types and applications. (newzy.net)
  • Continuous mammalian cell lines serve as potent tools for life sciences, aiding in the production of various drugs. (altogen.com)
  • The Vero Cell Media market shares of production and sales are evaluated along with the review of the production, capacity, sales, and revenue. (ahealthnewstoday.com)
  • Its main pathogenic property is the production of Shiga toxin (Stx), which inhibits the protein synthesis of host cells leading to cell death ( 3 , 4 ). (cdc.gov)
  • In our study, the optimum conditions (seeded cell number, incubated time period, 12-azidododecyl lactoside concentration and medium volume) for the production of Gb3 analogue were investigated. (biomedcentral.com)
  • The identification of an endoplasmic reticulum-Golgi intermediate compartment (ERGIC), defined by the 53-kDa transmembrane marker protein ERGIC-53, has added to the complexity of the exocytic pathway of higher eukaryotic cells. (uu.nl)
  • Also, Vero can serve as a host cell line for growing viruses or eukaryotic parasites (Trypanosomatids. (altogen.com)
  • Vero cells are used for multiple purposes, including screening for the toxins such as Escherichia coli. (altogen.com)
  • Vero cells are sensitive to Shiga toxins and are known to express the glycosides globotriaosyl ceramide (Gb3) and globotetraosyl ceramide (Gb4) on the surface of the plasma membrane. (biomedcentral.com)
  • A single NS2 mutation of K86R promotes PR8 vaccine donor virus growth in Vero cells. (sigmaaldrich.com)
  • In this study, a single mutation of K86R in the NS2 protein can sufficiently render the high-yielding property to the PR8 virus in Vero cells. (sigmaaldrich.com)
  • Mutations were also identified that modulated virus sensitivity to type I interferon-stimulation in Vero cells. (ajtmh.org)
  • sapiens-tc/GIN/2014/Makona-C05 virus (Rocky Mountain Laboratory, National Institutes of Health, Hamilton, MT, USA) was used to generate passage 2 virus stock in Vero E6 cells (Table 1). (thefreedictionary.com)
  • A fungal metabolite brefeldin A (BFA) was used to study virus-host interaction in glycoprotein processing in West Nile virus-infected Vero and C6/36 cells. (nus.edu.sg)
  • The results showed that in Vero cells, the transport of glycoproteins to the Golgi apparatus is important in West Nile virus infection. (nus.edu.sg)
  • Supernatants from infected cells protected Vero cells from c.p.e. and caused up to a 95% reduction of wild-type virus yield. (microbiologyresearch.org)
  • In Vero cells, all of the mutant viruses replicated to high titres comparable to the wild-type influenza B virus. (microbiologyresearch.org)
  • A novel influenza A virus mitochondrial protein that induces cell death. (microbiologyresearch.org)
  • b: Ultra-thin sections of infected Vero E6 cells displayed typical contrast-rich virus particles, organized as paracrystalline structures within the cytosol. (bioz.com)
  • With the help of the Vero platform, the timeline from receipt of the H1N1 virus by the laboratory to final release by the licensing authority was a mere 12 weeks. (vxpbiologics.com)
  • The major task this week will be picking pupae, but on Thursday, Friday, and Saturday, we will also be inoculating our cells with Zika virus. (caseynparker.com)
  • Typically, ultrastructural analysis is used to evaluate the virus-cell interaction. (virosin.org)
  • i would like to know whether the property of vero cell line and rota virus match with each other. (vaccinenation.org)
  • Zika virus NS4A and NS4B proteins deregulate Akt-mTOR signaling in human fetal neural stem cells to inhibit neurogenesis and induce autophagy. (nature.com)
  • This phlebovirus was isolated in Vero cells, confirmed by TEM and RT-PCR for the L segment of the virus, and named Viola phlebovirus. (springer.com)
  • African swine fever virus uses macropinocytosis to enter host cells. (nih.gov)
  • Whereas early studies have proposed that the virus enters cells through receptor-mediated endocytosis, the specific mechanism used by ASFV remains uncertain. (nih.gov)
  • The entry of virus into cells appears to directly stimulate dextran uptake, actin polarization and EGFR, PI3K-Akt, Pak1 and Rac1 activation. (nih.gov)
  • In conclusion, this study identifies for the first time the whole pathway for ASFV entry, including the key cellular factors required for the uptake of the virus and the cell signaling involved. (nih.gov)
  • Cells that surrounded the virus-infected cells showed translocation of β 4 subunit proteins to nuclei in response to spreading infection. (sciencemag.org)
  • Thus, in virus-infected cells, phosphorylation of Thr573 was not necessary to induce phosphorylation of Ser380. (qxmd.com)
  • The protein X4 of severe acute respiratory syndrome-associated coronavirus is expressed on both virus-infected cells and lung tissue of severe acute respiratory syndrome patients and inhibits growth of Balb/c 3T3 cell line. (qxmd.com)
  • The main objective was to develop a process with as little as possible of intermediate steps, especially omitting Tangential Flow Filtration (TFF) or other sample pre-treatments with high host-cell DNA and protein removal, as well as to achieve high binding capacity of the Influenza virus per mL of monolithic support. (biaseparations.com)
  • Our results showed that TMPRSS2 and MSPL played significant roles in the stages of cell-cell fusion and virus-cell fusion, whereas HAT and DESC1 exhibited weaker effects. (duhnnae.com)
  • The findings of this study support the theory that the Vero cell attenuated DR13 virus may be applied as an oral vaccine for inducing specific immunity in late-term pregnant sows with a high margin of protection against PEDV infection. (snu.ac.kr)
  • Vero E6, also known as Vero C1008 (ATCC No. CRL-1586) This line is a clone from Vero 76. (wikipedia.org)
  • To cultivate Vero cells in stirred-tank bioreactors, they require a growth matrix to attach to. (eppendorf.com)
  • The research covers the current market size of the Vero Cell Media market and its growth ratio based on 10-year history statistics along with the company profile of key members or manufacturers. (5gigs.com)
  • The in-depth information by segments of Vero Cell Media market helps monitor future profitability & to make critical decisions for growth. (5gigs.com)
  • Geographically, this report is scrutinized into several key Regions, with generation, consumption, revenue (million USD), and market share and growth rate of Vero Cell Media market in these regions, from 2012 to 2019, covering North America, Europe, China & Japan and its Share (%) and CAGR for the forecast period 2020 to 2029. (5gigs.com)
  • A Clear understanding of the Vero Cell Media market based on growth, constraints, opportunities, feasibility study. (newzy.net)
  • The SARS coronavirus nucleocapsid protein induces actin reorganization and apoptosis in COS-1 cells in the absence of growth factors. (qxmd.com)
  • It presents the Vero Cell Media market overview with growth analysis together with historical & futuristic costs. (ahealthnewstoday.com)
  • It also explains facts about the worldwide Vero Cell Media market and key pointers in terms of its growth and sales. (ahealthnewstoday.com)
  • So that they can plan their growth map of the Vero Cell Media industry for the coming years. (ahealthnewstoday.com)
  • Vero F6 was transfected via a concatenated plasmid with the gH gene after a copy of the HHV-1 glycoprotein-D (gD) promoter region. (wikipedia.org)
  • DNA plasmid expressing GAPDH or siRNA targeting GAPDH were transfected into VERO cells following Altogen Biosystems transfection protocol. (altogen.com)
  • The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus. (wikipedia.org)
  • It also showed that BFA could be used as a tool to understand further the trafficking of glycoprotein from the ER to Golgi in flavivirus infection in Vero cells. (nus.edu.sg)
  • Lastly, the AV529-19 Vero cells were shown to adhere effectively to insert membranes and form confluent monolayers. (uwaterloo.ca)
  • Lytic viruses were titrated in Vero monolayers and plotted in the table (n = 3). (nih.gov)
  • The results suggest that in the secretory pathway of Vero cells O-glycan initiation and sphingomyelin as well as glucosylceramide synthesis mainly occur beyond the ERGIC in the Golgi apparatus. (uu.nl)
  • Pakyawan Vero Cells Nakapagtuturo Sa Microcarriers na may mataas na kalidad bilang mababang presyo / murang, isa sa Vero Cells Nakapagtuturo Sa Microcarriers nangungunang tatak mula sa China, Changchun BCHT Biotechnology Co. (ccbcht.com)
  • The surface-area-to-volume ratio and scalability of Corning microcarriers make them an ideal choice for the expansion of Vero and MRC-5 cells. (corning.com)
  • The phase II study of inactivated vaccine (vero cell) against EV71 has completed last month in Jiangsu Province in China. (clinicaltrials.gov)
  • This report centers around Vero Cell Media volume and incentive at the global dimension, territorial dimension, and friends level. (5gigs.com)
  • The original cell line was named "Vero" after an abbreviation of verda reno, which means "green kidney" in Esperanto, while vero itself means "truth" in Esperanto. (wikipedia.org)
  • The whole genome sequence of a Vero cell line was determined by Japanese investigators in 2014. (wikipedia.org)
  • Ultimately a cell line is established from a single cell (monoclonal) in which all of the cells produce an identical antibody. (fsu.edu)
  • Use only Express Delivery (overnight) for cell line. (addexbio.com)
  • This is a cell line with the hypodiploid chromosome count. (addexbio.com)
  • This line is a clone from Vero 76. (alchetron.com)
  • Maintenance of Toxoplasma gondii in Vero cell line. (bvsalud.org)
  • Yasumura and Kawakita isolated these cells in 1962 and named the cell line as an abbreviation of "Verda reno" meaning "green kidney" in Esperanto. (altogen.com)
  • The Vero cell line is a mammalian cell line commonly used for research in the fields of virology, bacteriology, and toxicology. (altogen.com)
  • Also, the Vero cell line is an excellent host for studying a range of areas within the field of biomedical therapeutics. (altogen.com)
  • MTT assay was performed to assess viability of the normal kidney Vero cells treated with CDDP and/or AEC. (nih.gov)
  • The Vero Cell Media market covers the report likewise incorporates the top to bottom data in regards to the main manufacturers Biological Industries, Sigma-Aldrich, Lonza, Boster Bio, ATCC and Serana Bio . (5gigs.com)
  • The report carries an independent division of Vero Cell Media market key players Biological Industries, Lonza, Sigma-Aldrich, Boster Bio, Serana Bio, ATCC. (ahealthnewstoday.com)
  • ATCC offers OAT1 and OCT2 cells, which stably express soluble transporter proteins that play key roles in the kidney's clearance of drugs and endogenous compounds. (atcc.org)
  • ATCC offers both primary and hTERT-immortalized renal epithelial cells. (atcc.org)
  • The new Vero-SF-ACF (ATCC ® CCL-81.5™) is optimized to grow in the serum-free, animal componentfree media VeroPlus SFM (ATCC ® ACS-4001™) . (atcc.org)
  • ATCC has a large selection of cell lines, from an array of species, all particularly well-suited for the propagation of viruses. (atcc.org)
  • ATCC has the media you need to keep your cells happy, healthy, and behaving as expected. (atcc.org)
  • Partial depolymerization of microfilaments by treatment with latrunculin B at a concentration of 5 nM resulted in complete loss of stress fibers, yet cells slightly change their form, and microtubule system remained the same as in the control cells. (edu.kz)
  • To evaluate the role of microtubules in the process of cell retraction we depolymerized them with nocodazole taken at the concentration of 5 ng/ml. (edu.kz)
  • DENV infection and poly(I:C) also increased the concentration of intracellular Ca 2+ in these cells. (sciencemag.org)
  • A biphasic survival profile of cells was observed at 1-25 mu M concentration range following 96 h of incubation. (hacettepe.edu.tr)
  • Pretreatment of cells with ZnCl2 for 4 or 24 h provided significant protection against cobalt chloride-induced cytotoxicity when measured with MTT assay. (hacettepe.edu.tr)
  • Rasooly R, Hernlem B, He X, Friedman M. Non-Linear Relationships between Aflatoxin B 1 Levels and the Biological Response of Monkey Kidney Vero Cells. (mdpi.com)
  • Our recent study demonstrated that severe acute respiratory syndrome (SARS)-coronavirus (CoV) infection of monkey kidney Vero E6 cells induces phosphorylation and dephosphorylation of signaling pathways, resulting in apoptosis. (qxmd.com)
  • In this webinar, we demonstrate, how different process parameter can be monitored and controlled in a perfusion bioprocess to cultivate anchorage-dependent cells. (eppendorf.com)
  • However, thus far, a detailed electron microscopic study of the PPRV-cell interaction has not been performed. (virosin.org)
  • Among them, the trans -sialidase (TS) has arisen as one of particular relevance due to its effect on the immune system and involvement in the interaction/invasion of the host cells. (frontiersin.org)