Vero cells. Medical search

A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.

A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.

An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.

The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.

Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.

Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.

A species of MORBILLIVIRUS causing distemper in dogs, wolves, foxes, raccoons, and ferrets. Pinnipeds have also been known to contract Canine distemper virus from contact with domestic dogs.

Established cell cultures that have the potential to propagate indefinitely.

A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).

The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions.

A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It is closely related to SHIGA TOXIN produced by SHIGELLA DYSENTERIAE.

A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.

The type species of MORBILLIVIRUS and the cause of the highly infectious human disease MEASLES, which affects mostly children.

Proteins found in any species of virus.

Inoculation of a series of animals or in vitro tissue with an infectious bacterium or virus, as in VIRULENCE studies and the development of vaccines.

Process of growing viruses in live animals, plants, or cultured cells.

Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.

A species of the genus FLAVIVIRUS which causes an acute febrile and sometimes hemorrhagic disease in man. Dengue is mosquito-borne and four serotypes are known.

A protein phytotoxin from the seeds of Ricinus communis, the castor oil plant. It agglutinates cells, is proteolytic, and causes lethal inflammation and hemorrhage if taken internally.

The lone species of the genus Asfivirus. It infects domestic and wild pigs, warthogs, and bushpigs. Disease is endemic in domestic swine in many African countries and Sardinia. Soft ticks of the genus Ornithodoros are also infected and act as vectors.

Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.

A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It shares 50-60% homology with SHIGA TOXIN and SHIGA TOXIN 1.

A class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS. They include SHIGA TOXIN which is produced by SHIGELLA DYSENTERIAE and a variety of shiga-like toxins that are produced by pathologic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157.

A family of RNA viruses naturally infecting rodents and consisting of one genus (ARENAVIRUS) with two groups: Old World Arenaviruses (ARENAVIRUSES, OLD WORLD) and New World Arenaviruses (ARENAVIRUSES, NEW WORLD). Infection in rodents is persistent and silent. Vertical transmission is through milk-, saliva-, or urine-borne routes. Horizontal transmission to humans, monkeys, and other animals is important.

The use of techniques that produce a functional MUTATION or an effect on GENE EXPRESSION of a specific gene of interest in order to identify the role or activity of the gene product of that gene.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

A family of large icosahedral DNA viruses infecting insects and poikilothermic vertebrates. Genera include IRIDOVIRUS; RANAVIRUS; Chloriridovirus; Megalocytivirus; and Lymphocystivirus.

Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.

A genus of FLAVIVIRIDAE containing several subgroups and many species. Most are arboviruses transmitted by mosquitoes or ticks. The type species is YELLOW FEVER VIRUS.

A name for several highly contagious viral diseases of animals, especially canine distemper. In dogs, it is caused by the canine distemper virus (DISTEMPER VIRUS, CANINE). It is characterized by a diphasic fever, leukopenia, gastrointestinal and respiratory inflammation and sometimes, neurologic complications. In cats it is known as FELINE PANLEUKOPENIA.

Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.

A subgroup of the genus FLAVIVIRUS which comprises a number of viral species that are the etiologic agents of human encephalitis in many different geographical regions. These include Japanese encephalitis virus (ENCEPHALITIS VIRUS, JAPANESE), St. Louis encephalitis virus (ENCEPHALITIS VIRUS, ST. LOUIS), Murray Valley encephalitis virus (ENCEPHALITIS VIRUS, MURRAY VALLEY), and WEST NILE VIRUS.

Immunoglobulins produced in response to VIRAL ANTIGENS.

A plant family of the order Plumbaginales, subclass Caryophyllidae, class Magnoliopsida of shrubs and herbs. Some members contain ANTHOCYANINS and naphthaquinones.

The functional hereditary units of VIRUSES.

A family of viruses, mainly arboviruses, consisting of a single strand of RNA. Virions are enveloped particles 90-120 nm diameter. The complete family contains over 300 members arranged in five genera: ORTHOBUNYAVIRUS; HANTAVIRUS; NAIROVIRUS; PHLEBOVIRUS; and TOSPOVIRUS.

Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.

The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).

Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.

Arthropod-borne viruses. A non-taxonomic designation for viruses that can replicate in both vertebrate hosts and arthropod vectors. Included are some members of the following families: ARENAVIRIDAE; BUNYAVIRIDAE; REOVIRIDAE; TOGAVIRIDAE; and FLAVIVIRIDAE. (From Dictionary of Microbiology and Molecular Biology, 2nd ed)

Ribonucleic acid that makes up the genetic material of viruses.

The type (and only) species of RUBIVIRUS causing acute infection in humans, primarily children and young adults. Humans are the only natural host. A live, attenuated vaccine is available for prophylaxis.

A genus of gram-negative, aerobic, rod-shaped bacteria often surrounded by a protein microcapsular layer and slime layer. The natural cycle of its organisms generally involves a vertebrate and an invertebrate host. Species of the genus are the etiological agents of human diseases, such as typhus.

A plant genus of the family ASTERACEAE. The common name of thoroughwort is also used for other plants including EUPATORIUM; CHROMOLAENA, Hebeclinium and Koanophyllon. Eupolin is the aqueous extract of the leaves.

The type species of the FLAVIVIRUS genus. Principal vector transmission to humans is by AEDES spp. mosquitoes.

Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.

A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.

A species of CORONAVIRUS causing infections in chickens and possibly pheasants. Chicks up to four weeks old are the most severely affected.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Proteins that are coded by immediate-early genes, in the absence of de novo protein synthesis. The term was originally used exclusively for viral regulatory proteins that were synthesized just after viral integration into the host cell. It is also used to describe cellular proteins which are synthesized immediately after the resting cell is stimulated by extracellular signals.

Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.

The type species of RUBULAVIRUS that causes an acute infectious disease in humans, affecting mainly children. Transmission occurs by droplet infection.

The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.

A genus of protozoan parasites of the subclass COCCIDIA. Its species are parasitic in dogs, cattle, goats, and sheep, among others. N. caninum, a species that mainly infects dogs, is intracellular in neural and other cells of the body, multiplies by endodyogeny, has no parasitophorous vacuole, and has numerous rhoptries. It is known to cause lesions in many tissues, especially the brain and spinal cord as well as abortion in the expectant mother.

Deoxyribonucleic acid that makes up the genetic material of viruses.

A phenomenon in which infection by a first virus results in resistance of cells or tissues to infection by a second, unrelated virus.

A species of CORONAVIRUS causing atypical respiratory disease (SEVERE ACUTE RESPIRATORY SYNDROME) in humans. The organism is believed to have first emerged in Guangdong Province, China, in 2002. The natural host is the Chinese horseshoe bat, RHINOLOPHUS sinicus.

Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.

A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE), which is the etiological agent of Japanese encephalitis found in Asia, southeast Asia, and the Indian subcontinent.

A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.

Substances elaborated by viruses that have antigenic activity.

A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.

Specific hemagglutinin subtypes encoded by VIRUSES.

Proteins encoded by a VIRAL GENOME that are produced in the organisms they infect, but not packaged into the VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY.

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

A toxin produced by SHIGELLA DYSENTERIAE. It is the prototype of class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS.

Glycosphingolipids which contain as their polar head group a trisaccharide (galactose-galactose-glucose) moiety bound in glycosidic linkage to the hydroxyl group of ceramide. Their accumulation in tissue, due to a defect in ceramide trihexosidase, is the cause of angiokeratoma corporis diffusum (FABRY DISEASE).

A phenomenon manifested by an agent or substance adhering to or being adsorbed on the surface of a red blood cell, as tuberculin can be adsorbed on red blood cells under certain conditions. (Stedman, 25th ed)

A family of the order DIPTERA that comprises the mosquitoes. The larval stages are aquatic, and the adults can be recognized by the characteristic WINGS, ANIMAL venation, the scales along the wing veins, and the long proboscis. Many species are of particular medical importance.

An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.

A subfamily in the family CEBIDAE that consists of four genera: CALLITHRIX (marmosets), CALLIMICO (Goeldi's monkey), LEONTOPITHECUS (lion tamarins), and SAGUINUS (long-tusked tamarins). The members of this family inhabit the tropical forests of South and Central America.

The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.

A plant species of the genus MELIA, family MELIACEAE, which is toxic to insects. The name is very similar to Melia azadirachta (AZADIRACHTA).

Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.

Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.

The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)

The type species of PNEUMOVIRUS and an important cause of lower respiratory disease in infants and young children. It frequently presents with bronchitis and bronchopneumonia and is further characterized by fever, cough, dyspnea, wheezing, and pallor.

Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).

Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.

Multinucleated masses produced by the fusion of many cells; often associated with viral infections. In AIDS, they are induced when the envelope glycoprotein of the HIV virus binds to the CD4 antigen of uninfected neighboring T4 cells. The resulting syncytium leads to cell death and thus may account for the cytopathic effect of the virus.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

A group of acute infections caused by herpes simplex virus type 1 or type 2 that is characterized by the development of one or more small fluid-filled vesicles with a raised erythematous base on the skin or mucous membrane. It occurs as a primary infection or recurs due to a reactivation of a latent infection. (Dorland, 27th ed.)

A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.

Vaccines or candidate vaccines used to prevent infection with Japanese B encephalitis virus (ENCEPHALITIS VIRUS, JAPANESE).

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

A ubiquitously expressed complement receptor that binds COMPLEMENT C3B and COMPLEMENT C4B and serves as a cofactor for their inactivation. CD46 also interacts with a wide variety of pathogens and mediates immune response.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

A rare, slowly progressive encephalitis caused by chronic infection with the MEASLES VIRUS. The condition occurs primarily in children and young adults, approximately 2-8 years after the initial infection. A gradual decline in intellectual abilities and behavioral alterations are followed by progressive MYOCLONUS; MUSCLE SPASTICITY; SEIZURES; DEMENTIA; autonomic dysfunction; and ATAXIA. DEATH usually occurs 1-3 years after disease onset. Pathologic features include perivascular cuffing, eosinophilic cytoplasmic inclusions, neurophagia, and fibrous gliosis. It is caused by the SSPE virus, which is a defective variant of MEASLES VIRUS. (From Adams et al., Principles of Neurology, 6th ed, pp767-8)

The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.

An analog of DEOXYURIDINE that inhibits viral DNA synthesis. The drug is used as an antiviral agent.

Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.

Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (1/5424)

Herpes simplex virus type 1 (HSV-1) has many attractive features that suggest its utility for gene transfer to neurons. However, viral cytotoxicity and transient transgene expression limit practical applications even in the absence of viral replication. Mutant viruses deleted for the immediate early (IE) gene, ICP4, an essential transcriptional transactivator, are toxic to many cell types in culture in which only the remaining IE genes are expressed. In order to test directly the toxicity of other IE gene products in neurons and develop a mutant background capable of longterm transgene expression, we generated mutants deleted for multiple IE genes in various combinations and tested their relative cytotoxicity in 9L rat gliosarcoma cells, Vero monkey kidney cells, and primary rat cortical and dorsal root neurons in culture. Viral mutants deleted simultaneously for the IE genes encoding ICP4, ICP22 and ICP27 showed substantially reduced cytotoxicity compared with viruses deleted for ICP4 alone or ICP4 in combination with either ICP22, ICP27 or ICP47. Infection of neurons in culture with these triple IE deletion mutants substantially enhanced cell survival and permitted transgene expression for over 21 days. Such mutants may prove useful for efficient gene transfer and extended transgene expression in neurons in vitro and in vivo. (+info)

Rubella virus-induced apoptosis varies among cell lines and is modulated by Bcl-XL and caspase inhibitors. (2/5424)

Rubella virus (RV) causes multisystem birth defects in the fetuses of infected women. To investigate the cellular basis of this pathology, we examined the cytopathic effect of RV in three permissive cell lines: Vero 76, RK13, and BHK21. Electron microscopy and the TUNEL assay showed that the cytopathic effect resulted from RV-induced programmed cell death (apoptosis) in all three cell lines, but the extent of apoptosis varied among these cells. At 48 h postinfection, the RK13 cell line showed the greatest number of apoptotic cells, the Vero 76 cell line was approximately 3-fold less, and BHK21 had very few. An increased multiplicity of infection and longer time postinfection were required for the BHK21 cell line to reach the level of apoptotic cells in Vero 76 at 48 h. Purified RV induced apoptosis in a dose-dependent fashion, but not UV-inactivated RV or virus-depleted culture supernatant. Specific inhibitors of the apoptosis-specific proteases caspases reduced RV-induced apoptosis and led to higher levels of RV components in infected cells. To address the role of regulatory proteins in RV-induced apoptosis, the antiapoptotic gene Bcl-2 or Bcl-XL was transfected into RK13 cells. Although a high level of Bcl-2 family proteins was expressed, no protection was observed from apoptosis induced by RV, Sindbis virus, or staurosporine in RK13 cells. In BHK21 cells, however, increased expression of Bcl-XL protected cells from apoptosis. The observed variability in apoptotic response to RV of these cell lines demonstrates that programmed cell death is dependent on the unique properties of each cell and may be indicative of how selective organ damage occurs in a congenital rubella syndrome fetus. (+info)

CLIP-170 highlights growing microtubule ends in vivo. (3/5424)

A chimera with the green fluorescent protein (GFP) has been constructed to visualize the dynamic properties of the endosome-microtubule linker protein CLIP170 (GFP-CLIP170). GFP-CLIP170 binds in stretches along a subset of microtubule ends. These fluorescent stretches appear to move with the growing tips of microtubules at 0.15-0.4 microm/s, comparable to microtubule elongation in vivo. Analysis of speckles along dynamic GFP-CLIP170 stretches suggests that CLIP170 treadmills on growing microtubule ends, rather than being continuously transported toward these ends. Drugs affecting microtubule dynamics rapidly inhibit movement of GFP-CLIP170 dashes. We propose that GFP-CLIP170 highlights growing microtubule ends by specifically recognizing the structure of a segment of newly polymerized tubulin. (+info)

Susceptibilities of Mycobacterium tuberculosis and Mycobacterium avium complex to lipophilic deazapteridine derivatives, inhibitors of dihydrofolate reductase. (4/5424)

Twelve lipophilic 2,4-diamino-5-methyl-5-deazapteridine derivatives and trimethoprim were evaluated for activity against Mycobacterium tuberculosis and Mycobacterium avium in vitro. Six of the compounds had MICs of < or =12.8 mg/L and < or =1.28 mg/L against M. tuberculosis and M. avium, respectively; trimethoprim MICs were >128 mg/L and >12.8 but < or =128 mg/L, respectively. Two compounds, with either a 2-methyl-5-methoxy phenyl or 2-methoxy-5-trifluoromethyl phenyl linked at the 6-position of the deazapteridine moiety by a CH2NH bridge, had MICs of < or =0.13 mg/L against M. avium; the two compounds also had apparent I50 values for dihydrofolate reductase of 2 and 8 nM, respectively, compared with an I50 of 400 nM with trimethoprim. Four of the compounds were selectively toxic to mycobacteria as compared with Vero cells. These results demonstrated that lipophilic antifolates can be synthesized which are more active against mycobacteria than trimethoprim and which possess selective toxicity. (+info)

Mutations in the retinoblastoma protein-binding LXCXE motif of rubella virus putative replicase affect virus replication. (5/5424)

The rubella virus (RV)-encoded protein NSP90, which contains the retinoblastoma protein (Rb)-binding motif LXCXE, interacts with Rb and RV replication is reduced in cells lacking Rb. Whether the LXCXE motif of RV NSP90 itself is essential for Rb binding and virus replication is not known. Therefore, in the present study, the functional role of this motif was investigated by site-directed mutagenesis in a plasmid from which infectious RV RNA can be produced. Three critical mutations in the motif, two substitutions at the conserved cysteine residue (C --> G and C --> R) and a deletion of the entire motif, were created. A cell-free translated NSP90 C terminus polypeptide containing the deletion did not bind to Rb and a polypeptide carrying the C --> R substitution had barely detectable binding affinity for Rb. Rb binding by the C --> G mutant was reduced significantly compared to that of wild-type protein. Correlating with the binding results, mutant viruses containing the LXRXE and LXGXE motifs had a reduction in replication to < 0.5% and 47% of the wild-type, respectively, while deletion of the motif was found to be lethal. By the first serial passage, replication of the LXRXE-carrying virus had increased from < 0.5% to 2% of the wild-type. Sequencing of the genome of this virus revealed a nucleotide change that altered the motif from LXRXE to LXSXE, which is a known Rb-binding motif in two protein phosphatase subunits. Thus, our results clearly demonstrate that the LXCXE motif is required for efficient RV replication. (+info)

Characterization of the L gene and 5' trailer region of Ebola virus. (6/5424)

The nucleotide sequences of the L gene and 5' trailer region of Ebola virus strain Mayinga (subtype Zaire) have been determined, thus completing the sequence of the Ebola virus genome. The putative transcription start signal of the L gene was identical to the determined 5' terminus of the L mRNA (5' GAGGAAGAUUAA) and showed a high degree of similarity to the corresponding regions of other Ebola virus genes. The 3' end of the L mRNA terminated with 5' AUUAUAAAAAA, a sequence which is distinct from the proposed transcription termination signals of other genes. The 5' trailer sequence of the Ebola virus genomic RNA consisted of 676 nt and revealed a self-complementary sequence at the extreme end which may play an important role in virus replication. The L gene contained a single ORF encoding a polypeptide of 2212 aa. The deduced amino acid sequence showed identities of about 73 and 44% to the L proteins of Ebola virus strain Maleo (subtype Sudan) and Marburg virus, respectively. Sequence comparison studies of the Ebola virus L proteins with several corresponding proteins of other non-segmented, negative-strand RNA viruses, including Marburg viruses, confirmed a close relationship between filoviruses and members of the Paramyxovirinae. The presence of several conserved linear domains commonly found within L proteins of other members of the order Mononegavirales identified this protein as the RNA-dependent RNA polymerase of Ebola virus. (+info)

The herpes simplex virus type 1 regulatory protein ICP27 is required for the prevention of apoptosis in infected human cells. (7/5424)

The herpes simplex virus type 1 (HSV-1) ICP27 protein is an immediate-early or alpha protein which is essential for the optimal expression of late genes as well as the synthesis of viral DNA in cultures of Vero cells. Our specific goal was to characterize the replication of a virus incapable of synthesizing ICP27 in cultured human cells. We found that infection with an HSV-1 ICP27 deletion virus of at least three separate strains of human cells did not produce immediate-early or late proteins at the levels observed following wild-type virus infections. Cell morphology, chromatin condensation, and genomic DNA fragmentation measurements demonstrated that the human cells died by apoptosis after infection with the ICP27 deletion virus. These features of the apoptosis were identical to those which occur during wild-type infections of human cells when total protein synthesis has been inhibited. Vero cells infected with the ICP27 deletion virus did not exhibit any of the features of apoptosis. Based on these results, we conclude that while HSV-1 infection likely induced apoptosis in all cells, viral evasion of the response differed among the cells tested in this study. (+info)

Glycoprotein gL-independent infectivity of pseudorabies virus is mediated by a gD-gH fusion protein. (8/5424)

Envelope glycoproteins gH and gL, which form a complex, are conserved throughout the family Herpesviridae. The gH-gL complex is essential for the fusion between the virion envelope and the cellular cytoplasmic membrane during penetration and is also required for direct viral cell-to-cell spread from infected to adjacent noninfected cells. It has been proposed for several herpesviruses that gL is required for proper folding, intracellular transport, and virion localization of gH. In pseudorabies virus (PrV), glycoprotein gL is necessary for infectivity but is dispensable for virion localization of gH. A virus mutant lacking gL, PrV-DeltagLbeta, is defective in entry into target cells, and direct cell-to-cell spread is drastically reduced, resulting in only single or small foci of infected cells (B. G. Klupp, W. Fuchs, E. Weiland, and T. C. Mettenleiter, J. Virol. 71:7687-7695, 1997). We used this limited cell-to-cell spreading ability of PrV-DeltagLbeta for serial passaging of cells infected with transcomplemented virus by coseeding with noninfected cells. After repeated passaging, plaque formation was restored and infectivity in the supernatant was observed. One single-plaque isolate, designated PrV-DeltagLPass, was further characterized. To identify the mutation leading to this gL-independent infectious phenotype, Southern and Western blot analyses, radioimmunoprecipitations, and DNA sequencing were performed. The results showed that rearrangement of a genomic region comprising part of the gH gene into a duplicated copy of part of the unique short region resulted in a fusion fragment predicted to encode a protein consisting of the N-terminal 271 amino acids of gD fused to the C-terminal 590 residues of gH. Western blotting and radioimmunoprecipitation with gD- and gH-specific antibodies verified the presence of a gDH fusion protein. To prove that this fusion protein mediates infectivity of PrV-DeltagLPass, cotransfection of PrV-DeltagLbeta DNA with the cloned fusion fragment was performed, and a cell line, Nde-67, carrying the fusion gene was established. After cotransfection, infectious gL-negative PrV was recovered, and propagation of PrV-DeltagLbeta on Nde-67 cells produced infectious virions. Thus, a gDH fusion polypeptide can compensate for function of the essential gL in entry and cell-to-cell spread of PrV. (+info)

Vero cells are a lineage of cells used in cell cultures. The 'Vero' lineage was isolated from kidney epithelial cells extracted ... Vero E6, also known as Vero C1008 (ATCC No. CRL-1586) This line is a clone from Vero 76. Vero E6 cells show some contact ... F6B1.1) HeLa cells Immortalised cell line History and Characterization of the Vero Cell Line -- A Report prepared by CDR ... The genome analysis indicated that the Vero cell lineage is derived from a female Chlorocebus sabaeus. Vero cells are used for ...

https://en.wikipedia.org/wiki/Vero_cell

The test cultures cells that allow viral reproduction (e.g., Vero E6 cells). By varying antibody concentrations, researchers ... Nevertheless, memory cells including Memory B cells and Memory T cells can last much longer and may have the ability to reduce ... "Lack of Peripheral Memory B Cell Responses in Recovered Patients with Severe Acute Respiratory Syndrome: A Six-Year Follow-Up ... Leslie M (May 2020). "T cells found in coronavirus patients 'bode well' for long-term immunity". Science. 368 (6493): 809-810. ...

https://en.wikipedia.org/wiki/COVID-19_testing

"Baxter Sells Vero Cell Vaccines Platform". Genetic Engineering & Biotechnology News. Vol. 35, no. 2. 15 Dec 2014. p. 9. ... Baxter sold its vaccine production technology based on Vero cell culture to Nanotherapeutics. The sale included developed ... The new facility, once complete, will add cell therapy capabilities to Ology Bioservices' offerings. In mid-2016, ...

https://en.wikipedia.org/wiki/Ology_Bioservices

"Arenoviruses in Vero Cells: Ultrastructural Studies". Journal of Virology. 6 (4): 507-518. PETERS, C. J.; KUEHNE, R. W.; ...

https://en.wikipedia.org/wiki/Patricia_Ann_Webb

Initially, a sample of SARS-CoV-2 from China was used to grow large quantities of the virus using vero cells. From then on, the ... "COVID-19 Vaccine (Vero cell), Inactivated" (PDF). Sinovac. March 2021. Clinical Research Protocol for CoronaVac Phase III ... "EMA starts rolling review of COVID-19 Vaccine (Vero Cell) Inactivated". EMA - European Medicines Agency. Archived from the ... "WHO recommendation Sinovac COVID-19 vaccine (Vero Cell [Inactivated]) - CoronaVac". World Health Organization (WHO). 1 May 2021 ...

https://en.wikipedia.org/wiki/CoronaVac

It is produced in cultures of Vero cells. Modified vaccinia Ankara (also known as MVA): a highly attenuated (not virulent) ... On the other hand, the CEV is believed to play a role in cell-to-cell spread and the EEV is thought to be important for long ... Cell Stem Cell. 7 (5): 618-630. doi:10.1016/j.stem.2010.08.012. PMC 3656821. PMID 20888316. "Side Effects of Smallpox ... He was attempting to grow vaccinia virus on agar media in the absence of living cells when he noted that many colonies of ...

https://en.wikipedia.org/wiki/Vaccinia

Just subB is sufficient to cause vacuolation of vero cells. Neu5GC is not made by humans but is acquired from food sources such ... After their B subunit binds to receptors on the cell surface, the toxin is enveloped by the cell and transported inside either ... Once StxB targets a cancerous cell, it delivers the A subunit of the toxin which eventually kills the cancerous cell. Yet ... SubAB's target is in the endoplasmic reticulum of the cell and is brought into the cell through clathrin-mediated endocytosis. ...

https://en.wikipedia.org/wiki/AB5_toxin

"WHO recommendation Sinovac COVID-19 vaccine (Vero Cell [Inactivated]) - CoronaVac". World Health Organization (WHO). 1 May 2021 ...

https://en.wikipedia.org/wiki/Sinovac_Biotech

It is readily grown in monolayers of eukaryotic Vero cells. Serological evidence and PCR indicate that S. negevensis is ...

https://en.wikipedia.org/wiki/Simkaniaceae

"WHO recommendation Sinovac COVID-19 vaccine (Vero Cell [Inactivated]) - CoronaVac". World Health Organization (WHO). 1 May 2021 ... Vero cells)". Chinese Clinical Trial Registry. Retrieved 15 August 2020. Xia S, Zhang Y, Wang Y, Wang H, Yang Y, Gao GF, et al ...

https://en.wikipedia.org/wiki/List_of_COVID-19_vaccine_authorizations

... to increase the receptivity of Vero cells. Silencing its effect with siRNA prevented infection of Vero cells. TIM1 is expressed ... the cells survived and appeared impervious to the virus, further indicating that Ebola relies on NPC1 to enter cells; mutations ... focus on cell death". Cell Death and Differentiation. 22 (8): 1250-1259. doi:10.1038/cdd.2015.67. ISSN 1350-9047. PMC 4495366. ... surface peplomer and is endocytosed into macropinosomes in the host cell. To penetrate the cell, the viral membrane fuses with ...

https://en.wikipedia.org/wiki/Zaire_ebolavirus

Then, it was used to grow large quantities of the virus using vero cells. From then on, the viruses are soaked in beta- ... Its product name is SARS-CoV-2 Vaccine (Vero Cell), not to be confused with the similar product name of CoronaVac. Peer- ... The Sinopharm product is an inactivated vaccine called SARS-CoV-2 Vaccine (Vero Cell). Chen W, Al Kaabi N (18 July 2020). "A ... Yang Y. "A Study to Evaluate The Efficacy, Safety and Immunogenicity of Inactivated SARS-CoV-2 Vaccines (Vero Cell) in Healthy ...

https://en.wikipedia.org/wiki/Sinopharm_BIBP_COVID-19_vaccine

The fusion vectors are transfected in Vero cells (monkey kidney fibroblasts). Particularly interesting ORFs are also screened ... At the end of the live cell imaging, the cells can still be fixed and colocalisation experiments made. As the sequence of the ... The GFP-cDNA project documents the localisation of proteins to subcellular compartments of the eukaryotic cell applying ... and expressed in eukaryotic cells. Subsequently, the subcellular localisation of the fusion proteins is recorded by ...

https://en.wikipedia.org/wiki/GFP-cDNA

... purified chicken embryo cell vaccine (PCECV), developed in 1984; and a purified Vero cell rabies vaccine (PVCRV) developed in ... These include the human diploid cell vaccine (HDCV) first introduced in 1978; ...

https://en.wikipedia.org/wiki/Duck_embryo_vaccine

In Vero cells and LLC-MK2 cells, Lebombo virus causes lysis; however, it does not cause any cytopathic effect in C6/36 cells. ...

https://en.wikipedia.org/wiki/Lebombo_virus

"EMA starts rolling review of COVID-19 Vaccine (Vero Cell) Inactivated". European Medicines Agency (EMA) (Press release). 4 May ... Diamond MS, Pierson TC (May 2020). "The Challenges of Vaccine Development against a New Virus during a Pandemic". Cell Host & ... which increases the virus attachment to its target cells and might trigger a cytokine storm if a vaccinated person is later ...

https://en.wikipedia.org/wiki/History_of_COVID-19_vaccine_development

Newer vaccines, based on vero cells, are in development (as of 2018). Increases in cases of yellow fever in endemic areas of ... Others have noted that switching manufacturing processes to modern cell-culture technology might improve vaccine supply ... people with thymus disorders associated with abnormal immune cell function, people with primary immunodeficiencies, and anyone ...

https://en.wikipedia.org/wiki/Yellow_fever_vaccine

Ploske, Greg L. (2012-01-06). "A/H5N1 Prepandemic Influenza Vaccine (Whole Virion, Vero Cell-Derived, Inactivated) [Vepacel ... On the other hand, the flu virus strand for cell-based vaccine is grown using cultured cell of mammalian origins. As there are ... Furthermore, the cell culture technology was developed which allows virus growing outside the body for the first time. The ... When producing the strand of weakened or killed virus, egg-based or cell-based technology can be applied. For the egg-based ...

https://en.wikipedia.org/wiki/Type_A_influenza_vaccine

A follow-up study indicates that ciprofloxacin has potent effects on inhibiting SARS-CoV-2 in Vero E6 cells as measured by ... Several more recent primary publications include reports in Cell Molecular Cell, Developmental Cell, Journal of Cell Biology ... Ambroxol is another drug that has beneficial effects in Vero E6 cells. A comprehensive review with over 1,500 citations by ... Cell. 119 (6): 753-766. doi:10.1016/j.cell.2004.11.038. PMID 15607973. Singh, S. B.; Davis, A. S.; Taylor, G. A.; Deretic, V. ( ...

https://en.wikipedia.org/wiki/Vojo_Deretic

Vero cells - a monkey kidney cell line that arose by spontaneous immortalisation. Cellosaurus, knowledge base of cell lines ... HEK 293 cells - derived from human fetal cells. Jurkat cells - a human T lymphocyte cell line isolated from a case of leukemia ... A549 cells - derived from a cancer patient lung tumor. HeLa cells - a widely used human cell line isolated from cervical cancer ... Immortalised cell lines have also found uses in biotechnology. An immortalised cell line should not be confused with stem cells ...

https://en.wikipedia.org/wiki/Immortalised_cell_line

It is a mesophilic bacteria that can be grown on Vero cells. Similar to other Chlamydiales, it is commonly found in two ... Parachlamydia acanthamoebae has been shown to infect and multiply in simian and human cells. Experimental models were able to ... Their results also shows that the P. acanthamoebae cells continued to replicate within the macrophages, and eventually caused ... These researchers collected human macrophages and placed these cells on growth plates in conjunction with P. acanthamoebae. The ...

https://en.wikipedia.org/wiki/Parachlamydia_acanthamoebae

The vaccine candidate is produced with Bharat Biotech's in-house vero cell manufacturing platform that has the capacity to ... April 2018). "Enhancing viral vaccine production using engineered knockout vero cell lines - A second look". Vaccine. 36 (16): ... 2 was isolated by India's National Institute of Virology and used to grow large quantities of the virus using vero cells. From ... The study showed that Phase II trials had a higher immune response and induced T-cell response due to the difference in dosing ...

https://en.wikipedia.org/wiki/Covaxin

Vero cells are a continuous cell line derived from epithelial cells of the African green monkey kidney, and are widely used for ... Barrett, P. N.; Mundt, W.; Kristner, O.; Howard, M. K. (2009). "Vero cell platform in vaccine production: moving towards cell ... Similar cell lines include buffalo green monkey kidney and BS-C-1. Chlorocebus monkeys are an important model organism for ... Jasinska, Anna J.; Rostamian, Dalar; Davis, Ashley T.; Kavanagh, Kylie (2020). "Transcriptomic analysis of cell-free fetal RNA ...

https://en.wikipedia.org/wiki/Chlorocebus

The Parachlamydiaceae naturally infect amoebae and can be grown in cultured Vero cells. The Parachlamydiaceae are not ...

https://en.wikipedia.org/wiki/Parachlamydiaceae

On late July and early August 2021, Ho Chi Minh City received 2 million of the 5 million doses of the vero cell Sinopharm BIBP ... "Chính phủ đồng ý mua 20 triệu liều vaccine phòng COVID-19 Vero Cell". Vietnan Ministry of Health. September 23, 2021. "Thêm 8 ... Thanh Hoa authorities stopped injecting the remaining 43,000 doses of Vero Cell in storage and investigated the cause of this ... "Dừng tiêm lô vaccine Vero Cell sau khi ba người tử vong" (in Vietnamese). VnExpress. November 25, 2021. Retrieved November 25, ...

https://en.wikipedia.org/wiki/COVID-19_vaccination_in_Vietnam

1992). "Evaluation of Vero cell co-culture system for mouse embryos in various media". Human Reproduction. 7 (2): 276-80. doi: ... He also co-cultured human embryos with helper cells to promote growth. Cohen is known for the application of micromanipulation ...

https://en.wikipedia.org/wiki/Jacques_Cohen

It is based on a SA14-14-2 strain and cultivated in Vero cells. In September 2012, the Indian firm Biological E. Limited ... The other was an inactivated vaccine cultivated on primary hamster kidney cells (the Beijing-3 strain). The Beijing-3 strain ... launched an inactivated cell culture derived vaccine based on SA 14-14-2 strain which was developed in a technology transfer ...

https://en.wikipedia.org/wiki/Japanese_encephalitis_vaccine

These cells are typically Madin-Darby Canine Kidney cells, but others are also used including monkey cell lines pMK and Vero ... The virus in Ixiaro is grown in mammal cells ('Vero cells') under laboratory conditions. Pérez Rubio A, Eiros JM (2018). "Cell ... Verorab, developed by Sanofi Pasteur, is a mammalian vero cell based rabies vaccine approved by the World Health Organization. ... The Food and Drug Administration approved two mammalian vero cell based vaccines for rotavirus, Rotarix by GlaxoSmithKline and ...

https://en.wikipedia.org/wiki/Cell-based_vaccine

"Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells". Annals of ...

https://en.wikipedia.org/wiki/Artemisia_arborescens

"Inhibition of Dengue Virus Serotypes 1 to 4 in Vero Cell Cultures with Morpholino Oligomers". J. Virol. 79 (8): 5116-28. doi: ... HIV most heavily targets a specific type of lymphocyte known as "helper T cells", and identifies these target cells through T- ... Release of viral genes and possibly enzymes into the host cell. Replication of viral components using host-cell machinery. ... It was reported to induce rapid apoptosis selectively in virus-infected mammalian cells, while leaving uninfected cells ...

https://en.wikipedia.org/wiki/Antiviral_drug

PB has a face centered cubic lattice structure, with four iron III atoms per unit cell. "Soluble" PB crystals contain ... utrimque vero, universa res uberius explicatur atque confirmatur (in Latin). Haude. Woodward, J. (1724-1725). "Praeparatio ... Histopathology of the liver, showing a Kupffer cells with significant hemosiderin deposition next to a hepatocyte with ... leaving three such groups on average per unit cell. The empty nitrogen sites are filled with water molecules instead, which are ...

https://en.wikipedia.org/wiki/Prussian_blue

The virus exits the host cell by lysis, and viroporins. Horses serve as the natural host. ERBV's appear to infect most foals ... Vero), equine foetal kidney (EFK), and is able to infect humans. The RNA genome of the virion is inside the capsid that is ... The virion essentially is a nucleocapsid that is visible under an electron microscope and is able to infect cultured cells from ... Entry into the host cell is achieved by attachment of the virus to host receptors, which mediates endocytosis. Replication ...

https://en.wikipedia.org/wiki/Erbovirus

Furthermore, plant extracts of water spinach inhibit cancer cell growth of Vero, Hep-2 and A-549 cells, though have moderate ...

https://en.wikipedia.org/wiki/Ipomoea_aquatica

At Hell in a Cell on 5 June, Bálor teamed with AJ Styles and Liv Morgan to face The Judgment Day (Edge, Damian Priest and Rhea ... On 19 August 2019, Devitt married Mexican sports journalist Vero Rodríguez in a private ceremony in Tulum. They had confirmed ... Seth Rollins in a Hell in a Cell match, Goldberg vs. Bobby Lashley in a No Holds Barred match". Pro Wrestling Dot Net. ... Retrieved 30 June 2022.{{cite web}}: CS1 maint: url-status (link) Powell, Jason (5 June 2022). "WWE Hell in a Cell results: ...

https://en.wikipedia.org/wiki/Finn_Bálor

Manufacturing of vaccine for the Phase III trial was done by Merck, using the Vero cell line, which Merck already used to make ... Knowledge and expertise including but not limited to purification techniques, cell lines, materials, software codes and their ...

https://en.wikipedia.org/wiki/RVSV-ZEBOV_vaccine

T cells, B-cells and memory cells are stimulated upon encountering antigen in Peyer's patches. These cells then pass to the ... From p. 8: "Horum vero Plexuum facies modo in orbem concinnata; modo in Ovi aut Olivae oblongam, aliamve angulosam ac magis ... Among the mononuclear cells, CD4+/CD25+ (10%) cells and CD8+/CD25+ (5%) cells are more abundant in Peyer's patches than in the ... Peyer's patches are covered by a special follicle-associated epithelium that contains specialized cells called microfold cells ...

https://en.wikipedia.org/wiki/Peyer's_patch

VERO and NIH3T3 cell lines compared with the other cell lines. GRCh38: Ensembl release 89: ENSG00000139292 - Ensembl, May 2017 ... Paneth cells, enteroendocrine cells, goblet cells, tuft cells, columnar cells and the M cells over an adult's entire lifetime. ... ve stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro". Cell Stem Cell. 6 (1): 25-36. doi ... ve stem cells reside in the isthmus, the region between the pit cells and gland cells, where most cellular proliferation takes ...

https://en.wikipedia.org/wiki/LGR5

"Perdiamo un vero amico e un simbolo di Mandello" (in Italian) Kevin Conroy, the definitive voice of Batman in animation and ... Revolutionary Cells). Oleksandr Kostin [uk], 83, Ukrainian composer. Lê Lựu, 79, Vietnamese writer. Carlos Pacheco, 60, Spanish ...

https://en.wikipedia.org/wiki/Deaths_in_2022

Esparza, Kike (October 26, 2019). ""La casa de las flores 2" está entre azul y buenas noches; sí hace falta la Vero" ["La casa ... Season three begins at the moment season two ended, showing Paulina being taken to her prison cell. In prison, she meets a ... de las flores 2" is neither here nor there; it is missing Vero]. Rosa Distrito (in Spanish). Retrieved November 20, 2019. ...

https://en.wikipedia.org/wiki/Paulina_de_la_Mora

... the African monkey kidney cell line (Vero cells), human lung fibroblasts (MRC-5), and human epidermoid carcinoma cells (HEp-2 ... Many viruses can be grown in cell culture in the lab. To do this, the virus sample is mixed with cells, a process called ... Although different viruses often only grow in certain types of cells, there are cells that support the growth of a large ... the cells are successfully replicating the virus is to check for a change in cell morphology or for the presence of cell death ...

https://en.wikipedia.org/wiki/Laboratory_diagnosis_of_viral_infections

Vero cell line), which are then inactivated with formalin. The injected Salk vaccine confers IgG-mediated immunity in the ... Wahid R, Cannon MJ, Chow M (May 2005). "Virus-Specific CD4+ and CD8+ Cytotoxic T-Cell Responses and Long-Term T-Cell Memory in ... In 1960, the rhesus monkey kidney cells used to prepare the poliovirus vaccines were determined to be infected with the simian ... Eddy BE, Borman GS, Berkeley WH, Young RD (May 1961). "Tumors induced in hamsters by injection of rhesus monkey kidney cell ...

https://en.wikipedia.org/wiki/Polio_vaccine

Vero Pizzigoni, Enciclopedia universale SEDA dell'arte moderna, 1971, IDAF Aldo Calò, Ab Origine - presenze pugliesi nell'arte ... or in other works made with cells cultures in vitro, or in any experiments touching the boundaries of science. In his various ...

https://en.wikipedia.org/wiki/Giovanni_Valentini_(artist)

Vero, and L929. In contrast to Rickettsia species which reside in the nucleus of the host cell, O. tsutsugamushi mostly grows ... The bacterium is enclosed by a cell wall on the outside and cell membrane on the inside. The cell covering takes up stains such ... The cell wall is less rigid due to the absence of peptidoglycan, which is otherwise characteristic of the rigid cell walls of ... It forms another vesicle using the host cell membrane, gives rise to a small bud, and releases itself from the host cell ...

https://en.wikipedia.org/wiki/Orientia_tsutsugamushi

Swiss 3t3 cells MeSH A11.251.210.700.775.800 - 3t3-l1 cells MeSH A11.251.210.955 - vero cells MeSH A11.251.353.485 - hybridomas ... cho cells MeSH A11.251.210.505 - l cells (cell line) MeSH A11.251.210.520 - llc-pk1 cells MeSH A11.251.210.700 - 3t3 cells MeSH ... l cells MeSH A11.329.228.900 - 3t3 cells MeSH A11.329.228.900.080 - balb 3t3 cells MeSH A11.329.228.900.550 - nih 3t3 cells ... hela cells MeSH A11.251.210.190.400.500 - kb cells MeSH A11.251.210.190.465 - hl-60 cells MeSH A11.251.210.190.475 - ht29 cells ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(A11)

In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% ... "Reversible G1 arrest in the cell cycle of human lymphoid cell lines by dimethyl sulfoxide". Exp. Cell Res. 187 (1): 4-10. doi: ... In cell culture, DMSO is used to induce differentiation of P19 embryonic carcinoma cells into cardiomyocytes and skeletal ... DMSO may also be used as a cryoprotectant, added to cell media to reduce ice formation and thereby prevent cell death during ...

https://en.wikipedia.org/wiki/Dimethyl_sulfoxide

ACAM2000s virus is cultured in kidney epithelial cells (Vero cells) from an African green monkey. Efficacy and adverse reaction ... the virus seemed to move from cell to cell, but by around the 12th day, widespread lysis of infected cells occurred and the ... After entry of an infectious virion into a host cell, synthesis of viral mRNA can be detected within 20 minutes. About half of ... Poxviruses are unique among human DNA viruses in that they replicate in the cytoplasm of the cell rather than in the nucleus. ...

https://en.wikipedia.org/wiki/Smallpox

500,000 doses of Sinopharm's Vero Cell vaccine and over 500,000 syringes (donated by the Chinese government) arrived in Hanoi. ... particularly in lung cells. The Ministry of Health reduced home quarantine in April 2021 to seven days because it did not ...

https://en.wikipedia.org/wiki/COVID-19_pandemic_in_Vietnam

"Vitaherpavac is the first Russian herpes simplex virus vaccine obtained on the Vero B continuous cell line". Voprosy ... only the HSV-tk transfected Hela cells were killed by the granciclovir, leaving the nonviral cells unharmed. The Hela cells ... When Hela cells were transfected with the HSV-tk gene, and were then put in a culture with nontransfected cells, ... The miRNAs effect on apoptosis has affected cancer development by the regulation of cell proliferation, as well as cell ...

https://en.wikipedia.org/wiki/Herpes_simplex_research

These include: Virus isolation attempt in new born mice and cell culture (Vero Cells) Serological assay methods, such as HI ( ... This occurs when two genetically related viruses infect the same cell at the same time forming a progeny virus and this virus ... These findings confirmed the neurotropism of this virus, which means that this virus is capable of infecting nerve cells. ...

https://en.wikipedia.org/wiki/Oropouche_fever

The elimination of long distance charges between islands for calls originating from cell phones was introduced and a half ... including a 26 million dollar cable between Vero Beach, Florida and Eight Mile Rock owned by a consortium with Batelco and AT&T ...

https://en.wikipedia.org/wiki/BTC_(The_Bahamas)

Unable to take him down, he tricked him into re-entering his cell by chasing a hologram of Superman. Doomsday developed in one ... Director Zack Snyder confirmed during a watch party of Man of Steel on the social media site Vero that the broken moon visible ... Oz, opened all the cells to escape. Tim Drake from the "Titans Tomorrow" timeline, who had also been imprisoned there, came to ... Damaging his rocket-propelled prison cell enough before it could leave the planet's orbit, Doomsday went on a collision-course ...

https://en.wikipedia.org/wiki/Doomsday_(DC_Comics)

The cytotoxic concentrations of the essential oil in the leaves for HeLa and Vero cells (266.8 ± 46.5 and 312.1 ± 42.5 μg mL−1 ... of Eugenia calycina shows high activity against the larvae of Aedes aegypti but has lower cytotoxicity against mammalian cells ...

https://en.wikipedia.org/wiki/Eugenia_calycina

... , Coonoor is producing Vero Cell Derived Rabies Vaccine for Human use and DPT group vaccines ... it measures the ability of rabies specific antibodies to neutralize rabies virus and prevent the virus from infecting cells. ...

https://en.wikipedia.org/wiki/Pasteur_Institute_of_India

"Fini, nuovo duello con il Senatur "Il vero suicidio è negare i diritti"". Corriere della Sera (in Italian). Archived from the ... became the most influential representative of the socially liberal wing of the party due to his progressive views on stem cell ...

https://en.wikipedia.org/wiki/Future_and_Freedom

LCMV can be grown in a variety of cell lines including BHK21, L and Vero cells, and it may be identified with immuno- ... The results showed that CTL killed only the infected cells from strain A, and they did not lyse uninfected cells or infected ... In addition, a better understanding of CD-4 T cell memory is also a result of studies with LCMV and will continue to contribute ... It is then endocytosed into a vesicle inside the host cell and creates a fusion of the virus and vesicle membranes. The ...

https://en.wikipedia.org/wiki/Lymphocytic_choriomeningitis

... and 36 after dose 1 of a 2-dose primary series of inactivated Vero cell culture--derived JE vaccine (JE-VC [manufactured as ... The updated recommendations included information on use of a new inactivated, Vero cell culture--derived JE vaccine (JE-VC [ ... Effect of pre-existing anti-tick-borne encephalitis virus immunity on neutralising antibody response to the Vero cell-derived, ... Long-term immunogenicity of the new Vero cell-derived, inactivated Japanese encephalitis virus vaccine IC51: six and 12 month ...

https://www.cdc.gov/mmwr/preview/mmwrhtml/mm6020a5.htm?s_cid=mm6020a5_w

Antiviral activity against Human herpesvirus 1 infected African green monkey Vero cells after 66 hr by viral plaque reduction ...

https://pubchem.ncbi.nlm.nih.gov/bioassay/1087252

COVID-19 Vaccine (Vero Cell), Inactivated. Product Overview. Type: COVID-19 Vaccine (Vero Cell), Inactivated ...

https://extranet.who.int/pqweb/node/17454

Nepal Has Brought First Batch Of 4 Million Vero Cell Vaccine Purchases From China ... Nepal Has Brought First Batch Of 4 Million Vero Cell Vaccine Purchases From China. Nepal Has Brought First Batch Of 4 Million ... A wide body airplane, A 330, of the Nepal Airlines Corporation laden with 809,600 doses of Vero Cell landed in Kathmandu at 6: ... Earlier, China had donated 2 million doses of Vero Cell to Nepal. Nepal had also received 348 thousand doses of vaccine under ...

https://www.spotlightnepal.com/2021/07/10/nepal-has-brought-first-batch-4-million-vero-cell-vaccine-purchases-china/

Title : GRADE evidence for inactivated Vero cell culture-derived JE vaccine (JE-VC; Ixiaro) in children Personal Author(s) : ... GRADE evidence for inactivated Vero cell culture-derived JE vaccine (JE-VC; Ixiaro) in children. ... GRADE evidence for inactivated Vero cell culture-derived JE vaccine (JE-VC; Ixiaro) in children ...

https://stacks.cdc.gov/view/cdc/76408

Yet I suspect many of their users do not necessarily know how Vero cells came to be or what their name means. As with all cell ... Vero cells are one of the mainstays of mammalian cell culture. ... lines, knowing the intricacies (and limitations of a cell… ... Daniel BojarLeave a Comment on Article about Vero cells Article about Vero cells. Widely used in vaccine production and ... Yet I suspect many of their users do not necessarily know how Vero cells came to be or what their name means. As with all cell ...

https://dbojar.com/2020/12/15/article-about-vero-cells/

of Microbiology, School of Medicine, Chiba University, 1988. Description: 234 pSubject(s): Vero cellsNLM classification: QH 585 ... Vero cells : origin, properties and biomedical applications / edited by Bunsiti Simizu, Toyozo Terasima. Contributor(s): Simizu ...

https://kohahq.searo.who.int/cgi-bin/koha/opac-search.pl?q=au:%22Simizu%2C%20Bunsiti.%22

Srinivasa H, Mithal S, Balaya S. Maintenance of Toxoplasma gondii in Vero cell line. Indian Journal of Medical Research. 1982 ...

https://imsear.searo.who.int/handle/123456789/23189

Toxicity effect of mixture of selected Malaysia herbs in cultured Vero and WRL-68 cells ... Toxicity effect of mixture of selected Malaysia herbs in cultured Vero and WRL-68 cells. In: IIUM Research, Invention and ...

http://irep.iium.edu.my/35110/

... while the cytotoxicity assay against Vero cells, as determined by the MTT reduction method, showed a CC50 value of 132 μg/ml, ... of the essential oil obtained from leaves of Artemisia arborescens against HSV-1 and HSV-2 The cytotoxicity in Vero cells was ... seems to be particularly interesting in consideration of its ability to inactivate the virus and to inhibit the cell-to-cell ... by yield reduction assay was observed against HSV-1 at higher concentrations when added to cultures of infected cells. No ...

https://ann-clinmicrob.biomedcentral.com/articles/10.1186/1476-0711-6-10

In addition Vero cells were cultivated in a 7-1 bioreactor in batch mode on 3 gl(-1) Cytodex1, and infected at cell seeding at ... After nine passages the virus was able to correctly replicate in Vero cells, virus titer achieved was 10(5.875) TCID50 (median ... Cell infection at a MO! of 0.005 concurrently with cell seeding showed the best results in terms of specific virus productivity ... Sheep pox virus initially adapted to replicate in primary lamb kidney cells was adapted to Vero cells by serial passages in ...

https://hal-riip.archives-ouvertes.fr/pasteur-01375060

TaggedVero Cell Rabies Vaccine IndustryVero Cell Rabies Vaccine MarketVero Cell Rabies Vaccine Market Report ... Vero Cell Rabies Vaccine Market 2022 Size, Growth Factors & Forecast. by Disha Rathor. November 17, 2022. ... The vero cell rabies vaccine market is witnessing substantial growth due to the increased government measures toward preventing ... This section of the vero cell rabies vaccine market report provides detailed data on the segments at country and regional level ...

https://articleblock.com/business/vero-cell-rabies-vaccine-market-2022-size-growth-factors-forecast/

Vero Cells Cell system (a) Virus passage history (b) Evidence of Infection ... Vero (CL) Vero 3 3 3+ 5-6 1-2mm 7.0 dex ...

https://wwwn.cdc.gov/Arbocat/VirusDetails.aspx?ID=31&SID=6

Vero Cell Assay. Expression of Stx was examined by Vero cell cytotoxic assay. Of the 30 strains examined, all were cytotoxic to ... The initial procedure for preparation of cell-free culture supernatant and cell lysate was the same as for the Vero cell assay ... Vero Cell Assay. Preparation of Cell-Free Culture Filtrates. STEC strains were cultured in L-broth (Difco), at 37°C overnight ... The cytotoxic effect of STEC strains was assayed on Vero cells in 96-well flat-bottom tissue culture plates (NUNC, Intermade, ...

https://wwwnc.cdc.gov/eid/article/8/1/01-0104

C-methylribonucleosides and ribavirin against the Zika virus in Vero cells were confirmed using our developed method. To ... Here, we describe a fast and label-free phenotypic high-content imaging assay to detect cells affected by the virus-induced CPE ... High-throughput drug screening efforts focusing on indirect readouts of cell viability are prone to a higher frequency of false ... the activity of the compound was confirmed in human fetal neural stem cells (NSCs). The described technique can be easily ...

https://pubmed.ncbi.nlm.nih.gov/30061280/

Examination of vesicular stomatitis virus-induced morphology changes in individual Vero cells by qMod microscopy. March 23, ... We assessed characteristics of infected Vero cells over the course of infection, obtained feature measurements of individual ... and infection in Vero cells was examined by microscopy using the Ovizio qMod camera and OsOne software. ... cells and examined very early biophysical distinctions of infected cells.. Process Development, Ology Bioservices, Alachua, FL ...

https://ovizio.com/examination-of-vesicular-stomatitis-virus-induced-morphology-changes-in-individual-vero-cells-by-qmod-microscopy/

Cordycepin inhibits virus replication in dengue virus-infected vero cells. May 22, 2021. ... Alpha-mangostin inhibits dengue virus production and pro-inflammatory cytokine/chemokine expression in dendritic cells.May 31, ... Triphala in traditional ayurvedic medicine inhibits dengue virus infection in Huh7 hepatoma cells.Nov 27, 2021. ... Pubmed Data : Mol Cell Biochem. 2020 Jan ;464(1-2):169-180. Epub 2019 Nov 22. PMID: 31758375 ...

https://greenmedinfo.com/disease/dengue

Evolution of Avian coronavirus (AvCoV) in BHK-21 and VERO cells Paulo Eduardo Brandão, Beatriz Alcântara Leite, Sueli Akemi ...

https://www.revistas.usp.br/bjvras/issue/view/11491

... study aimed to evaluate the cytotoxic activity of the extracts and fractions of Eriocaulon cinereum against HeLa and Vero cell ... Saponins from Plumeria acuminata Ait induce cell growth inhibition and apoptosis of oral squamous carcinoma cells ... OSCC cells seeded at 2 × 104 cells/well in a 96-well plate were treated with saponins from P. acuminata Ait and cisplatin in ... HEK293/NF-κBluc cells were pretreated for 30 min with H. sucuuba extract (60 μg·mL-1), plumericin (5 μμ), parthenolide (5 μμ), ...

https://www.researchgate.net/profile/Nanang-Fakhrudin

One-week intramuscular or intradermal pre-exposure prophylaxis with human diploid cell vaccine or Vero cell rabies vaccine, ...

https://m.amedeo.com/35933278

5. Recommendations for Use of a Booster Dose of Inactivated Vero Cell Culture-Derived Japanese Encephalitis Vaccine - Advisory ... Recommendations for Use of a Booster Dose of Inactivated Vero Cell Culture-Derived Japanese Encephalitis Vaccine - Advisory ... approved an inactivated Vero cell culture-derived JE vaccine (manufactured as Ixiaro) for use in adults aged ≥17 years. ... Data on the need for and timing of booster doses of inactivated Vero cell culture-derived Japanese encephalitis vaccine (IXIARO ...

https://www.cdc.gov/media/mmwrnews/2011/0526.html

IPV grown in Vero cells. ++ IPV grown in Vero cells and administered through double-barrelled syringe with DTP vaccine. && IPV ... The viruses are grown on Vero cells, a continuous line of monkey kidney cells, by the microcarrier method. After concentration ... among recipients of an IPV produced in MRC-5 cells (POLIOVAX{TM})(37). In contrast, all studies using the IPV produced in Vero ... The viruses are propagated in monkey kidney cell culture. Since it was licensed in the United States in 1963, OPV has been the ...

https://wonder.cdc.gov/wonder/prevguid/m0046568/m0046568.asp

Biological: Inactivated SARS-CoV-2 Vaccine (Vero cell) The inactivated SARS-CoV-2 vaccine (vero cell) was manufactured by ... Participants will receive 2 doses of the inactivated SARS-CoV-2 vaccine (Vero cell) according to the immunization schedule of ... A Randomized, Double-Blinded, Placebo Controlled Phase III Clinical Trial of SARS-CoV-2 Vaccine, Inactivated (Vero Cell) in ... Biological: Inactivated SARS-CoV-2 Vaccine (Vero cell) Biological: Placebo Phase 3 ...

https://clinicaltrials.gov/ct2/show/NCT04659239

ACAM2000 is grown in African green monkey kidney (Vero) cells and tested to be free of known adventitious agents (4). Safety ... African Green Monkey kidney (Vero) cells, HEPES, human serum albumin, sodium chloride, neomycin, polymyxin B, Glycerin, phenol ... Are these vaccines made with African Green Monkey kidney cells and aborted human fetal cells really used in the United States? ... Vero) cells and tested to be free of adventitious agents. ACAM2000 is provided as a lyophilized preparation of purified live ...

https://www.newstarget.com/2017-03-09-cdc-confirms-natural-news-100-correct-on-report-of-african-green-monkey-kidney-cells-used-in-vaccines.html

... proved very high in Vero E6 cells, intermediate in HEK293T cells, and lower in Calu-3 cells, as described by others (28, 49). ... explaining their emergence in Vero cells. To evaluate how S1/S2 pre-cleavage impacts viral entry into Calu-3 or Vero E6 cells, ... To conclude, Calu-3 cells are suitable to assess viral entry via TMPRSS2 and other TTSPs, while Vero E6 cells are useful to ... Calu-3 cell entry (28). On the other hand, entry into Vero E6 cells was more efficient (4-fold, P , 0.0001) for SARS-1-S- ...

https://www.biorxiv.org/content/10.1101/2020.11.09.374603v1.full

Cytotoxicity to Vero cells was assayed as described (4). The strains were characterized as sorbitol-negative O157:H7, ...

https://wwwnc.cdc.gov/eid/article/8/4/01-0490_article

The distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus. ... Transfection with a human SLAM complementary DNA enables non-susceptible cell lines to bind measles virus, support measles ... which is expressed on all nucleated cells; however, most clinical isolates of measles virus cannot use CD46 as a receptor5. ... Figure 2: Infectivities of pseudotype viruses on B95a, Vero and transfected CHO cells.. ...

https://www.nature.com/articles/35022579?error=cookies_not_supported&code=ff0b71e9-ef9f-48e0-b1c1-8d08041ad970

Were working closely with the CDC on best practices to promote the health of our guests and crew. See our frequently asked questions.

https://www.princess.com/plan/cruise-with-confidence/cruise-health/frequently-asked-questions/au-cruises/?accordion=covid-test-prior-to-boarding

These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, ... Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS ... Chloroquine is effective in preventing the spread of SARS CoV in cell culture. ... We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. ...

https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-2-69

The updated recommendations included information on use of a new inactivated, Vero cell culture--derived JE vaccine (JE-VC [manufactured as Ixiaro]) that was licensed in the United States in 2009. (cdc.gov)
Over 809 thousand doses of Vero Cell, COVID-19 vaccine developed and manufactured by Sinopharm, has been brought in Kathmandu this morning. (spotlightnepal.com)
Widely used in vaccine production and research regarding viruses, Vero cells are one of the mainstays of mammalian cell culture. (dbojar.com)
COVID-19 Impact Analysis on Vero Cell Rabies Vaccine Market report published by Value Market Research provides a detailed market analysis comprising market size, share, value, growth and trends for the period 2022-2028. (articleblock.com)
The vero cell rabies vaccine market is witnessing substantial growth due to the increased government measures toward preventing the disease. (articleblock.com)
Furthermore, increasing research and development activities and a rise in veterinary healthcare spending are also propelling the market growth for the vero cell rabies vaccine. (articleblock.com)
Additionally, these tools also give an inclusive assessment of each segment in the global market of vero cell rabies vaccine. (articleblock.com)
The growth and trends of vero cell rabies vaccine industry provide a holistic approach to this study. (articleblock.com)
This section of the vero cell rabies vaccine market report provides detailed data on the segments at country and regional level, thereby assisting the strategist in identifying the target demographics for the respective product or services with the upcoming opportunities. (articleblock.com)
This section covers the regional outlook, which accentuates current and future demand for the Vero Cell Rabies Vaccine market across North America, Europe, Asia-Pacific, Latin America, and Middle East & Africa. (articleblock.com)
One-week intramuscular or intradermal pre-exposure prophylaxis with human diploid cell vaccine or Vero cell rabies vaccine, followed by simulated post-exposure prophylaxis at one year: A phase III, open-label, randomized, controlled trial to assess im Quiambao BP, Lim JG, Bosch Castells V, Augard C, et al. (amedeo.com)
One-week intramuscular or intradermal pre-exposure prophylaxis with human diploid cell vaccine or Vero cell rabies vaccine, followed by simulated post-exposure prophylaxis at one year: A phase III, open-label, randomized, controlled trial to assess im Vaccine 2022 Aug 3. (amedeo.com)
Data on the need for and timing of booster doses of inactivated Vero cell culture-derived Japanese encephalitis vaccine (IXIARO) were not available when the vaccine was licensed. (cdc.gov)
This is a randomized, double-blinded, placebo controlled phase III clinical trial to evaluate the efficacy, safety and immunogenicity of SARS-CoV-2 Vaccine, Inactivated (Vero Cell) in adults aged 18 years and above after 2-dose schedule. (clinicaltrials.gov)
Participants will receive 2 doses of the inactivated SARS-CoV-2 vaccine (Vero cell) according to the immunization schedule of D0, D14. (clinicaltrials.gov)
The inactivated SARS-CoV-2 vaccine (vero cell) was manufactured by IMBCAMS. (clinicaltrials.gov)
In addition to the CDC confirming Natural News is 100% correct through the documents listed above, the vaccine insert sheet itself also describes the use of African Green Monkey kidney cells . (newstarget.com)
ACAM2000, Smallpox (Vaccinia) Vaccine, Live, is a live vaccinia virus derived from plaque purification cloning from Dryvax® (Wyeth Laboratories, Marietta, PA, calf lymph vaccine, New York City Board of Health Strain) and grown in African Green Monkey kidney (Vero) cells and tested to be free of adventitious agents. (newstarget.com)

Bead enzyme-linked immunosorbent assay and Vero cell assay were performed to detect and evaluate the cytotoxic effect of the Shiga toxins produced by the strains. (cdc.gov)
The IC 50 values, determined by plaque reduction assay, were 2.4 and 4.1 μg/ml for HSV-1 and HSV-2, respectively, while the cytotoxicity assay against Vero cells, as determined by the MTT reduction method, showed a CC 50 value of 132 μg/ml, indicating a CC 50 /IC 50 ratio of 55 for HSV-1 and 32.2 for HSV-2. (biomedcentral.com)
A poor activity determined by yield reduction assay was observed against HSV-1 at higher concentrations when added to cultures of infected cells. (biomedcentral.com)
Here, we describe a fast and label-free phenotypic high-content imaging assay to detect cells affected by the virus-induced CPE using automated imaging and analysis. (nih.gov)
The described technique can be easily leveraged as a primary screening assay for profiling of the activities of large compound libraries against ZIKV and can be expanded to other ZIKV strains and other cell lines displaying morphological changes upon ZIKV infection. (nih.gov)
and before inoculating the first dose, 6 months and 12 months after the whole-course immunization, blood samples will be taken for detecting specific T cells with the ELISPOT assay with an aim to evaluate immunogenicity and immune persistence. (clinicaltrials.gov)
Validation of a cell-based assay for detection of active Shiga toxins produced by Escherichia coli in water. (usda.gov)
The study used an infectivity assay on Vero cells to measure inactivation the SARS-CoV-2 under UV-C LED irradiation. (thomasnet.com)

The cytotoxicity in Vero cells was evaluated by the MTT reduction method. (biomedcentral.com)
Cytotoxicity to Vero cells was assayed as described ( 4 ) . (cdc.gov)
The cytotoxicity on VERO cells was also investigated revealing that the organometallic complex [Au(damp)(dmtsc)] was the most selective compound for MDA-MB-231, although not the most active. (unesp.br)

The cytotoxic effect and cell cycle analysis of mouse melanoma cells (B16F10) and human melanoma cells (MeWo) were assessed using the ImageXpress Micro XLS Widefield High-Content Analysis System. (mdpi.com)
The results showed that hexadecyl pyridinium chloride has no obvious cytotoxic effect on Vero cells in the concentration range of 0.0125-0.05 mg/mL. (growkudos.com)

Cell infection at a MO! (archives-ouvertes.fr)
The recombinant VSV has been genetically altered to express the LASV Josiah glycoprotein (VSVΔG/LASVGP), and infection in Vero cells was examined by microscopy using the Ovizio qMod camera and OsOne software. (ovizio.com)
We assessed characteristics of infected Vero cells over the course of infection, obtained feature measurements of individual cells and examined very early biophysical distinctions of infected cells. (ovizio.com)
Figure 3: Measles virus infection of CHO cells expressing SLAM or CD46. (nature.com)
We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. (biomedcentral.com)
Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. (biomedcentral.com)
Together with data presented here, showing virus inhibition in cell culture by chloroquine doses compatible with patient treatment, these features suggest that further evaluation of chloroquine in animal models of SARS-CoV infection would be warranted as we progress toward finding effective antivirals for prevention or treatment of the disease. (biomedcentral.com)
A , B ) Vero E6 cells cultured in 24-well plates and treated only with medium (M) or with different concentrations of RBV (≤ 50 μg/mL) 24 hr before ( A ) or 2 hr after ( B ) infection by TCMV. (ajtmh.org)
C ) Vero E6 cells treated only with medium (M) or with medium added to RBV (50 μg/mL) 24 or 48 hours after infection by TCMV. (ajtmh.org)
D ) Vero E6 cells cultured in 24-well plates and treated only with medium (M), M added to guanosine (G), or M added to RBV, or M plus RBV and G 24 hr before infection by TCMV. (ajtmh.org)
A - D ) The overlay was removed 9 days after infection, cells were stained with naphthol blue black, and plaque-forming units (PFU) were counted. (ajtmh.org)
Alisporivir inhibits MERS- and SARS-coronavirus replication in cell culture, but not SARS-coronavirus infection in a mouse model. (lumc.nl)
To evaluate the ability of A3 enzymes in editing the genomes of MPXV, HeLa (Henrietta Lacks, human epithelial cell line) cells were transfected with the six aforementioned cytidine deaminases, followed by the 2022 MPXV strain infection. (socialworker-findoffice.com)
Later, for subsequent infection of a new Vero E6 cell culture flask, 100 µl of the clear supernatant was used. (lightprogress.com)

The following article originally appeared on June 15, 2020 in The Cell Culture Dish here . (corning.com)

Figure 2: Infectivities of pseudotype viruses on B95a, Vero and transfected CHO cells. (nature.com)
We also observed abundant production of virus-derived siRNAs against two other viruses in Lutzomyia Lulo cells. (plos.org)
The cytoplasmic replication of +RNA viruses can be explosive, with dramatic consequences for the architecture and functioning of the infected cell. (lumc.nl)
Gamma-clade viruses replicated to higher levels in mammalian cells than pandemic clade. (elifesciences.org)
After isolating the virus, the researchers used Vero cells - considered the most suitable system for primary isolation and cultivation of viruses - to culture the strain and grow virus stocks for sequence analysis. (naturalnews.com)

Sheep pox virus initially adapted to replicate in primary lamb kidney cells was adapted to Vero cells by serial passages in monolayer cultures. (archives-ouvertes.fr)

Objective: This study aimed to evaluate the cytotoxic activity of the extracts and fractions of Eriocaulon cinereum against HeLa and Vero cell lines,which represent cervical cancer and normal cells, respectively. (researchgate.net)
WB: THP-1, HAP1, PANC-1 and HeLa cell lysate. (abcam.com)

the previously reported antiviral activities of 2'- C -methylribonucleosides and ribavirin against the Zika virus in Vero cells were confirmed using our developed method. (nih.gov)
Of the 1,942 compounds tested, 21 exhibited a substantial antiviral activity in Vero-81 cells. (bvsalud.org)

Mycoplasma contamination is indeed a key factor to evaluate during a cell culture projects, as it can influence cell behavior, morphology and function. (corning.com)
The report further stated that the viral particles in the Vero cells had a similar morphology to coronaviruses. (naturalnews.com)

In agreement with this hypothesis, pre-treatment of cells with dsRNA against VSV was able to inhibit viral replication while knock-down of the central siRNA component, Argonaute-2, led to increased virus levels. (plos.org)
U.S.A., 83: 2787-91 (1986) that an eight residue oligomer complementary to the acceptor splice junction of Herpes Simplex Virus-1 (HSV-1) immediate early mRNAs 4 and 5 inhibits viral replication with little, if any, deleterious effect on host-cell macromolecular metabolism and growth rate. (justia.com)
After invasion of a new host cell by these virus particles, the transactivating components interact with certain regions of the viral DNA sequences and stimulate transcription of the adjacent downstream genes, which code for proteins needed by the virus for replication. (justia.com)
Tandem mass spectrometry identified over 500 viral peptides and 44 phosphopeptides in virus-infected cells, covering almost all proteins predicted to be encoded by the SARS-CoV-2 genome, including peptides unique to the deleted variant of the S glycoprotein. (biomedcentral.com)
Studies have reported that the locus of the human-origin A3 enzyme codes for six functional cytidine deaminases (A-3A, -3B, -3C, -3F, -3G, and -3H), described initially as intrinsic cell limiting factors against viral organisms through cytidine to uracil deamination in ssDNA (single-stranded deoxyribonucleic acid). (socialworker-findoffice.com)
The anti-viral ability of silver antibacterial materials In the article "Efficient and rapid cracking of SARS coronavirus and other microorganisms by metal catalysts", the research team compressed silver / alumina into discs and dropped Vero cells that support SARS coronavirus proliferation On the disc, the virus toxicity on the plate was compared after 5 min and 20 min, respectively. (olansiglobal.com)

The anti-inflammatory activity may be justified by high inhibition of T cell proliferation. (biomedcentral.com)
Its inhibition in human ES cells results in enhanced spontaneous differentiation. (silverchair.com)

Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips. (cellsignal.com)
We used direct RNA sequencing to determine the transcriptome of SARS-CoV-2 grown in Vero E6 cells which is widely used to propagate the novel coronavirus. (biomedcentral.com)

Consuat, vel illum dolore eu feugiat nulla facilisis at vero eros et accumsan et iusto. (shefeelsaliveinthemountains.com)
Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu feugiat nulla facilisis at vero eros et accumsan et iusto odio dignissim qui blandit praesent luptatum delenit augue duis dolore zzril te feugait nulla facilisi. (chrisseyner.fr)

Some of the arguments backing this assertion included wildly uninformed opinions such as, "there's no way the CDC would approve monkey kidney cells being injected into humans. (newstarget.com)
As Natural News correctly reported, one of the many animal-derived ingredients used in vaccines and openly admitted by the CDC is African Green Monkey kidney cells . (newstarget.com)
Now, CDC has confirmed the Natural News story to be 100% accurate and true through several documents posted on the CDC website which openly admit that African Green Monkey kidney cells are used in vaccines approved by the CDC for immunizations across America. (newstarget.com)
Note the inclusion of African Green Monkey kidney cells in the list. (newstarget.com)
Are these vaccines made with African Green Monkey kidney cells and aborted human fetal cells really used in the United States? (newstarget.com)
This page from the FDA lists all the vaccines which are "licensed for use in the United States," and it includes the vaccines containing African Green Monkey kidney cells and the WI-38 aborted human fetal cells. (newstarget.com)
This toxin was lethal to Vero cells (a line of kidney cells isolated from the African green monkey), and so was termed verotoxin. (medscape.com)

also known as CDw150), a recently discovered membrane glycoprotein expressed on some T and B cells 6 , is a cellular receptor for measles virus, including the Edmonston strain. (nature.com)
The strain was produced in Vero E6 cells, and the passage 2 stock was obtained using plaque-forming assays. (socialworker-findoffice.com)

Activation by these proteases requires pre-cleavage of the SARS-2-S S1/S2 cleavage loop, and both its furin motif and extended loop length proved critical to achieve virus entry into airway epithelial cells. (biorxiv.org)
Transcriptional signatures of primary normal human mammary epithelial cells in response to diesel particulate extract detected with DNA microarrays. (cdc.gov)

Health hazard evaluation report: HETA-2005-0167-2983, Indian River Memorial Hospital, Center for Emotional and Behavioral Health, Vero Beach, Florida. (cdc.gov)
Dean Wellness Institute has been serving Vero Beach, Florida and the surrounding areas for over 20 years. (deanwellnessinstitute.com)

Its main pathogenic property is the production of Shiga toxin (Stx), which inhibits the protein synthesis of host cells leading to cell death ( 3 , 4 ). (cdc.gov)
In fully expanded leaves the protein is immunodetectable mostly in a cell-wall- enriched fraction. (microbiologyresearch.org)
Movement of fluorescein-isothiocyanate-labelled dex- tran with a mean molecular mass of 10000 Da, and an approximate Stokes' radius of 2·3 nm, was detected between cells of the 3a protein transgenic plants, but not in the control plants. (microbiologyresearch.org)
These results are consistent with the idea that the CMV 3 a protein is involved in the modification of plasmodesmata and, therefore, in the cell-to-cell spread of the virus. (microbiologyresearch.org)
An N- proximal sequence of the alfalfa mosaic virus movement protein is necessary for association with cell walls in transgenic plants. (microbiologyresearch.org)
Cell-to-cell trafficking of macromolecules through plasmodesmata potentiated by the red clover necrotic mosaic virus movement protein. (microbiologyresearch.org)
A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. (microbiologyresearch.org)
Description: Host cell factor 2(HCFC2) is a cytoplasmic protein. (gentaurtop.com)

A narrative review of the migration and invasion features of non-small cell lung cancer cells upon xenobiotic exposure: insights from in vitro studies. (ulusofona.pt)
Citotoxicity evaluation of three dental adhesives on vero cells in vitro . (bvsalud.org)
In vitro assays against three tumor cell lines (B16-F10, HCT-8 and MDA-MB-231) showed that the gold(III) complexes display remarkable antiproliferative effects on human breast adenocarcinoma cell line with influence of the co-ligands. (unesp.br)

However, antibodies in the body are not secreted by circulating memory B cells in the blood but rather principally by plasma cells in the bone marrow. (frontiersin.org)

Kobune, F., Sakata, H. & Sugiura, A. Marmoset lymphoblastoid cells as a sensitive host for isolation of measles virus. (nature.com)

In addition Vero cells were cultivated in a 7-1 bioreactor in batch mode on 3 gl(-1) Cytodex1, and infected at cell seeding at a MO! (archives-ouvertes.fr)
Esco Aster, a leading independent contract development and manufacturing organization underpinned by its patent Tide Motion bioreactor technology, has signed a cooperation agreement with the Max Planck Institute (MPI) for Dynamics of Complex Technical Systems, Magdeburg, Germany to develop new techniques for cell-culture based virus production of human flavivirus vaccines. (journalforclinicalstudies.com)
The purpose of the project is to culture Vero cells, using a packed bed bioreactor to a high cell density, and produce live attenuated yellow fever virus (YFV). (journalforclinicalstudies.com)

After nine passages the virus was able to correctly replicate in Vero cells, virus titer achieved was 10(5.875) TCID50 (median tissue culture infective dose) ml(-1). (archives-ouvertes.fr)
Chloroquine is effective in preventing the spread of SARS CoV in cell culture. (biomedcentral.com)
Laboratory and cell culture contamination is a serious risk for research labs, process development labs and biomanufacturing. (corning.com)
Contamination can compromise cell culture and experiment results, thus wasting valuable resources conducting experiments that ultimately aren't accurate. (corning.com)
Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture. (lumc.nl)
The reassortants are propagated in Vero cells using standard cell culture techniques in the absence of antifungal agents. (rxlist.com)
He has over 18 years experience in teaching Biotechnology Engineering Laboratory IV, Bioprocess Facility Design and Regulatory, Biopharmaceutical Manufacturing, Cell Culture Engineering and Biomass Energy, Design and Optimization Process Biotechnology, Biochemical Engineering Fundamental, Process Plant Design & Analysis. (iium.edu.my)

Plasmodesmata between sieve elements and companion cell of extrafascicular stem phloem of Cucurbita maxima permit passage of 3 kDa probe. (microbiologyresearch.org)

High-throughput drug screening efforts focusing on indirect readouts of cell viability are prone to a higher frequency of false positives in cases where the virus is viable in the cell but the cytopathic effect (CPE) is reduced or delayed. (nih.gov)
Transfection with a human SLAM complementary DNA enables non-susceptible cell lines to bind measles virus, support measles virus replication and develop cytopathic effects. (nature.com)

Hospital Diagnosis is commonly preparation of a stool filtrate, which is applied to a monolayer of or travel exposures) the such as Vero cells. (techhunt2025.com)

The humoral response plays an indispensable role in ebolavirus immunity, based on studies of mAbs isolated from the antibody genes in peripheral blood circulating ebolavirus-specific human memory B cells. (frontiersin.org)
Here we exploited both single-cell antibody gene sequencing and proteomic sequencing approaches to assess the composition of the ebolavirus glycoprotein (GP)-reactive antibody repertoire in the plasma of an EVD survivor. (frontiersin.org)
Using mass spectrometric analysis of the corresponding GP-specific plasma IgG, we found that only a portion of the large B cell antibody repertoire was represented in the plasma. (frontiersin.org)

To evaluate the effect EE and fractions on cell proliferation, human lymphocytes were stimulated with phytohemagglutinin and analyzed using flow cytometry. (biomedcentral.com)
Inflammation in the tumor microenvironment aids proliferation and survival of malignant cells, promotes angiogenesis and metastasis, subverts adaptive immune responses, and alters responses to hormones and chemotherapeutic agents [ 6 ]. (biomedcentral.com)
Wound healing is an extremely complex process consists of hemostasis, inflammation, cell proliferation and scar tissue remodeling phases. (fabad.org.tr)

Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu feugiat nulla facilisis at vero eros. (shefeelsaliveinthemountains.com)

No. 4,757,055 discloses certain oligonucleoside alkyl and aryl phosphonates that are complementary to base sequences "foreign" to mammalian cells and that hinder the replication and/or translation of such foreign base sequences. (justia.com)

The distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus. (nature.com)

The mode of action of the essential oil as antiherpesvirus agent seems to be particularly interesting in consideration of its ability to inactivate the virus and to inhibit the cell-to-cell virus diffusion. (biomedcentral.com)
of 0.005 concurrently with cell seeding showed the best results in terms of specific virus productivity. (archives-ouvertes.fr)
Figure 1: Measle virus binding to CHO cells expressing SLAM or CD46. (nature.com)
These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. (biomedcentral.com)
Increase in plasmodesmatal permeability during cell-to-cell spread of tobacco rattle virus from individually inoculated cells. (microbiologyresearch.org)
these cells were positive for West Nile Virus by RT-PCR, immunofluorescence, and electron microscopy. (cdc.gov)
It has also been shown that sequences that are complementary to regions immediately adjacent to the tRNA Lys primer binding site in human T-cell lymphotropic virus type III (HTLV-III) inhibited replication of the virus [Zamecnik et al. (justia.com)
A CELL LINE derived from the kidney of the African green (vervet) monkey, ( CHLOROCEBUS AETHIOPS ) used primarily in virus replication studies and plaque assays. (bvsalud.org)
At 20 minutes, the virus was not found to have toxicological effects on cells. (olansiglobal.com)

the activity of the compound was confirmed in human fetal neural stem cells (NSCs). (nih.gov)

Sirtuins might therefore be of therapeutic significance, as they are overexpressed in cancer cells, and sirtuin inhibitors inhibit the development of thymic lymphomas in murine models. (silverchair.com)

When the silver ions are aggregated and reach a certain limit, they penetrate the cell wall and enter the interior, staying on the cytoplasmic membrane, inhibiting the activity of enzymes in the cytoplasmic membrane Cause the death of microorganisms such as bacteria. (olansiglobal.com)
Coxiella burnetii bacteria (green) inside a Vero cell (orange). (pressboltnews.com)

As with all cell lines, knowing the intricacies (and limitations of a cell line is crucial to getting the most out of using it for research or production. (dbojar.com)
IMSEAR at SEARO: Maintenance of Toxoplasma gondii in Vero cell line. (who.int)
Srinivasa H, Mithal S, Balaya S. Maintenance of Toxoplasma gondii in Vero cell line. (who.int)
β -Sitosterol-3- O - β -D-glucopyranoside exhibited high cytotoxic activity (IC 50 = 1.279 μg/mL) against HCT-116 cell line. (biomedcentral.com)
All the synthesized conjugates reveal antiproliferative properties against A431 (squamous skin) cancer cell line with potency higher than that of 5-fluorouracil. (elsevier.com)
Many of the synthesized agents also exhibit promising antiproliferative properties against HCT116 (colon) cancer cell line, of which 5o and 5c are the most effective with 12.9, 9.8 folds potency compared with Sunitinib. (elsevier.com)
Promising activity is also shown against MCF7 (breast) cancer cell line with 1.19, 1.12 folds relative to 5-fluorouracil. (elsevier.com)

Also there are tissue engineering products made of polymeric scaffolds which keratinocytes and fibroblast cells are cultured on. (fabad.org.tr)

The goal of the conference was to promote scientific collaboration and synergy between stem cell researchers worldwide and those in the EuroSTELLS consortia(an initiative of the European Science Foundation EUROCORES Programme), and to stimulate discussion of the latest results in the field of stem cell niches. (silverchair.com)
Researchers at the University of California San Diego (UCSD) developed a new biomaterial that promotes cell and tissue repair. (massdevice.com)

The results showed that the toxicity of SARS coronavirus in Vero cells decreased to a very low level at 5 minutes of exposure. (olansiglobal.com)

Lymphocytes are important cell populations involved in chronic inflammation. (biomedcentral.com)

PI-flow cytometry of compound 5c supports the arrest of cell cycle at G1-phase. (elsevier.com)
However, compound 5o and Sunitinib arrest the cell cycle at S-phase. (elsevier.com)

That's why, after my HEK293 article last month, I have worked on an article about Vero cells which I am very happy to share here! (dbojar.com)

Next, SARS-2-S proved efficiently activated by TMPRSS13, besides the previously identified host cell protease TMPRSS2, which may broaden the cell tropism of SARS-CoV-2. (biorxiv.org)

We propose that the principal famotidine mechanism of action for COVID-19 involves on-target histamine receptor H 2 activity, and that development of clinical COVID-19 involves dysfunctional mast cell activation and histamine release. (researchsquare.com)
Much current interest and excitement surrounding stem cells and their niches pertains to their potential clinical utility in transplantation and regenerative medicine settings. (silverchair.com)

SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology. (lumc.nl)

We first identified 1,512 GP-specific mAb variable gene sequences from single cells in the memory B cell compartment. (frontiersin.org)
The limited availability of stem cells within niches also contributes to how the size of a stem cell compartment is determined. (silverchair.com)

Despite intensive research into stem cells, we still understand little of what defines `stemness' molecularly. (silverchair.com)

Damage to endothelial cells is the primary event in the pathogenesis of hemolytic-uremic syndrome (HUS). (medscape.com)

Figure 4: Flow cytometry analysis of the cell-surface expression of SLAM. (nature.com)
Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco cells. (microbiologyresearch.org)

The cytotoxic activity was evaluated using MTT colorimetric method against MDA-MB-231, MCF-7, HCT-116, and Vero cells. (biomedcentral.com)

Anatomy8

Organisms33

Diseases3

Chemicals and Drugs28

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes12

Information Science3

Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (1/5424)

Rubella virus-induced apoptosis varies among cell lines and is modulated by Bcl-XL and caspase inhibitors. (2/5424)

CLIP-170 highlights growing microtubule ends in vivo. (3/5424)

Susceptibilities of Mycobacterium tuberculosis and Mycobacterium avium complex to lipophilic deazapteridine derivatives, inhibitors of dihydrofolate reductase. (4/5424)

Mutations in the retinoblastoma protein-binding LXCXE motif of rubella virus putative replicase affect virus replication. (5/5424)

Characterization of the L gene and 5' trailer region of Ebola virus. (6/5424)

The herpes simplex virus type 1 regulatory protein ICP27 is required for the prevention of apoptosis in infected human cells. (7/5424)

Glycoprotein gL-independent infectivity of pseudorabies virus is mediated by a gD-gH fusion protein. (8/5424)

Vero Cells

Cercopithecus aethiops

Diphtheria Toxin

Virus Replication

Cytopathogenic Effect, Viral

Viral Plaque Assay

Distemper Virus, Canine

Cell Line

Haplorhini

Herpesvirus 1, Human

Shiga Toxin 1

Simplexvirus

Measles virus

Viral Proteins

Serial Passage

Virus Cultivation

Cytotoxins

Dengue Virus

Ricin

African Swine Fever Virus

Bacterial Toxins

Shiga Toxin 2

Shiga Toxins

Arenaviridae

Reverse Genetics

Molecular Sequence Data

Iridoviridae

Viral Envelope Proteins

Flavivirus

Distemper

Antiviral Agents

Encephalitis Viruses, Japanese

Antibodies, Viral

Plumbaginaceae

Genes, Viral

Bunyaviridae

Enterotoxins

Neutralization Tests

Kidney

Arboviruses

RNA, Viral

Rubella virus

Rickettsia

Chromolaena

Yellow fever virus

Defective Viruses

Cricetinae

Infectious bronchitis virus

Base Sequence

Immediate-Early Proteins

Receptors, Virus

Mumps virus

Virulence

Neospora

DNA, Viral

Viral Interference

SARS Virus

Cell Fusion

Encephalitis Virus, Japanese

Brefeldin A

Antigens, Viral

L Cells (Cell Line)

Hemagglutinins, Viral

Viral Nonstructural Proteins

Fluorescent Antibody Technique

Shiga Toxin

Trihexosylceramides

Hemadsorption

Culicidae

Inclusion Bodies, Viral

Callitrichinae

Virion

Melia azedarach

Microscopy, Electron

Gene Expression Regulation, Viral

Dogs

Respiratory Syncytial Virus, Human

Viral Structural Proteins

Viral Vaccines

Giant Cells