AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsHumanitiesInformation Science
UrotheliumUrinary BladderUroplakin IIUroplakin IIIUrinary Bladder NeoplasmsCarcinoma, Transitional CellUrinary Bladder DiseasesUrologic NeoplasmsCystitisUroplakin IbUrinationUrinary TractUreterCystitis, InterstitialUroplakin IaUrinary Bladder, OveractiveCryoultramicrotomyUrogenital NeoplasmsAdministration, IntravesicalButylhydroxybutylnitrosamineCacodylic AcidPurinergic P2Y Receptor AgonistsUrethraUrodynamicsEpitheliumTetraspaninsKeratins, Type IMuscle, SmoothLewis Blood-Group SystemHyperplasiaKidney PelvisUropathogenic Escherichia coliCystectomyMicroscopy, Electron, ScanningKeratin-13UrineMucous MembraneImmunohistochemistryKeratin-20Urinary Bladder, NeurogenicUrinary Bladder CalculiPapillomaMuscarinic AntagonistsRats, Inbred F344Receptors, Purinergic P2X3ArchivesBiological Science DisciplinesPeriodicals as TopicPubMedDirectories as TopicGingival RecessionGingivoplastyUrinary Tract InfectionsConnective Tissue
Urothelium: The epithelial lining of the URINARY TRACT.Urinary Bladder: A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION.Uroplakin II: A uroplakin subtype that heterodimerizes with UROPLAKIN IA to form a component of the asymmetric unit membrane found in urothelial cells.Uroplakin III: A uroplakin subtype that heterodimerizes with UROPLAKIN IB to form a component of the asymmetric unit membrane found in urothelial cells.Urinary Bladder Neoplasms: Tumors or cancer of the URINARY BLADDER.Carcinoma, Transitional Cell: A malignant neoplasm derived from TRANSITIONAL EPITHELIAL CELLS, occurring chiefly in the URINARY BLADDER; URETERS; or RENAL PELVIS.Urinary Bladder Diseases: Pathological processes of the URINARY BLADDER.Urologic Neoplasms: Tumors or cancer of the URINARY TRACT in either the male or the female.Cystitis: Inflammation of the URINARY BLADDER, either from bacterial or non-bacterial causes. Cystitis is usually associated with painful urination (dysuria), increased frequency, urgency, and suprapubic pain.Uroplakin Ib: A tetraspanin domain-containing uroplakin subtype. It heterodimerizes with UROPLAKIN III to form a component of the asymmetric unit membrane found in urothelial cells.Urination: Discharge of URINE, liquid waste processed by the KIDNEY, from the body.Urinary Tract: The duct which coveys URINE from the pelvis of the KIDNEY through the URETERS, BLADDER, and URETHRA.Ureter: One of a pair of thick-walled tubes that transports urine from the KIDNEY PELVIS to the URINARY BLADDER.Cystitis, Interstitial: A condition with recurring discomfort or pain in the URINARY BLADDER and the surrounding pelvic region without an identifiable disease. Severity of pain in interstitial cystitis varies greatly and often is accompanied by increased urination frequency and urgency.Uroplakin Ia: A tetraspanin domain-containing uroplakin subtype. It heterodimerizes with UROPLAKIN II to form a component of the asymmetric unit membrane found in urothelial cells.Urinary Bladder, Overactive: Symptom of overactive detrusor muscle of the URINARY BLADDER that contracts with abnormally high frequency and urgency. Overactive bladder is characterized by the frequent feeling of needing to urinate during the day, during the night, or both. URINARY INCONTINENCE may or may not be present.Cryoultramicrotomy: The technique of using a cryostat or freezing microtome, in which the temperature is regulated to -20 degrees Celsius, to cut ultrathin frozen sections for microscopic (usually, electron microscopic) examination.Urogenital Neoplasms: Tumors or cancer of the UROGENITAL SYSTEM in either the male or the female.Administration, Intravesical: The instillation or other administration of drugs into the bladder, usually to treat local disease, including neoplasms.Butylhydroxybutylnitrosamine: A substituted carcinogenic nitrosamine.Cacodylic Acid: An arsenical that has been used as a dermatologic agent and as an herbicide.Purinergic P2Y Receptor Agonists: Compounds that bind to and stimulate PURINERGIC P2Y RECEPTORS. Included under this heading are agonists for specific P2Y receptor subtypes.Urethra: A tube that transports URINE from the URINARY BLADDER to the outside of the body in both the sexes. It also has a reproductive function in the male by providing a passage for SPERM.Urodynamics: The mechanical laws of fluid dynamics as they apply to urine transport.Epithelium: One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.Tetraspanins: A large superfamily of cell surface membrane proteins characterized by their four transmembrane domains. They play a role in a variety of processes such as cellular adhesion and motility. They may be involved in the organization of cell surface MEMBRANE MICRODOMAINS that regulate the activation of LEUKOCYTES.Keratins, Type I: A keratin subtype that includes keratins that are generally smaller and more acidic that TYPE II KERATINS. Type I keratins combine with type II keratins to form keratin filaments.Muscle, Smooth: Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)Lewis Blood-Group System: A group of dominantly and independently inherited antigens associated with the ABO blood factors. They are glycolipids present in plasma and secretions that may adhere to the erythrocytes. The phenotype Le(b) is the result of the interaction of the Le gene Le(a) with the genes for the ABO blood groups.Hyperplasia: An increase in the number of cells in a tissue or organ without tumor formation. It differs from HYPERTROPHY, which is an increase in bulk without an increase in the number of cells.Kidney Pelvis: The flattened, funnel-shaped expansion connecting the URETER to the KIDNEY CALICES.Uropathogenic Escherichia coli: Strains of Escherichia coli that preferentially grow and persist within the urinary tract. They exhibit certain virulence factors and strategies that cause urinary tract infections.Cystectomy: Used for excision of the urinary bladder.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Keratin-13: A type I keratin that is found associated with the KERATIN-4 in the internal stratified EPITHELIUM. Defects in gene for keratin 13 cause HEREDITARY MUCOSAL LEUKOKERATOSIS.Urine: Liquid by-product of excretion produced in the kidneys, temporarily stored in the bladder until discharge through the URETHRA.Mucous Membrane: An EPITHELIUM with MUCUS-secreting cells, such as GOBLET CELLS. It forms the lining of many body cavities, such as the DIGESTIVE TRACT, the RESPIRATORY TRACT, and the reproductive tract. Mucosa, rich in blood and lymph vessels, comprises an inner epithelium, a middle layer (lamina propria) of loose CONNECTIVE TISSUE, and an outer layer (muscularis mucosae) of SMOOTH MUSCLE CELLS that separates the mucosa from submucosa.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Keratin-20: A type I keratin expressed predominately in gastrointestinal epithelia, MERKEL CELLS, and the TASTE BUDS of the oral mucosa.Urinary Bladder, Neurogenic: Dysfunction of the URINARY BLADDER due to disease of the central or peripheral nervous system pathways involved in the control of URINATION. This is often associated with SPINAL CORD DISEASES, but may also be caused by BRAIN DISEASES or PERIPHERAL NERVE DISEASES.Urinary Bladder Calculi: Stones in the URINARY BLADDER; also known as vesical calculi, bladder stones, or cystoliths.Papilloma: A circumscribed benign epithelial tumor projecting from the surrounding surface; more precisely, a benign epithelial neoplasm consisting of villous or arborescent outgrowths of fibrovascular stroma covered by neoplastic cells. (Stedman, 25th ed)Muscarinic Antagonists: Drugs that bind to but do not activate MUSCARINIC RECEPTORS, thereby blocking the actions of endogenous ACETYLCHOLINE or exogenous agonists. Muscarinic antagonists have widespread effects including actions on the iris and ciliary muscle of the eye, the heart and blood vessels, secretions of the respiratory tract, GI system, and salivary glands, GI motility, urinary bladder tone, and the central nervous system.Rats, Inbred F344Receptors, Purinergic P2X3: A purinergic P2X neurotransmitter receptor involved in sensory signaling of TASTE PERCEPTION, chemoreception, visceral distension, and NEUROPATHIC PAIN. The receptor comprises three P2X3 subunits. The P2X3 subunits are also associated with P2X2 RECEPTOR subunits in a heterotrimeric receptor variant.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Gingival Recession: Exposure of the root surface when the edge of the gum (GINGIVA) moves apically away from the crown of the tooth. This is common with advancing age, vigorous tooth brushing, diseases, or tissue loss of the gingiva, the PERIODONTAL LIGAMENT and the supporting bone (ALVEOLAR PROCESS).Gingivoplasty: Surgical reshaping of the gingivae and papillae for correction of deformities (particularly enlargements) and to provide the gingivae with a normal and functional form, the incision creating an external bevel. (Dorland, 28th ed)Urinary Tract Infections: Inflammatory responses of the epithelium of the URINARY TRACT to microbial invasions. They are often bacterial infections with associated BACTERIURIA and PYURIA.Connective Tissue: Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.

Superimposed histologic and genetic mapping of chromosome 9 in progression of human urinary bladder neoplasia: implications for a genetic model of multistep urothelial carcinogenesis and early detection of urinary bladder cancer. (1/920)

The evolution of alterations on chromosome 9, including the putative tumor suppressor genes mapped to the 9p21-22 region (the MTS genes), was studied in relation to the progression of human urinary bladder neoplasia by using whole organ superimposed histologic and genetic mapping in cystectomy specimens and was verified in urinary bladder tumors of various pathogenetic subsets with longterm follow-up. The applicability of chromosome 9 allelic losses as non-invasive markers of urothelial neoplasia was tested on voided urine and/or bladder washings of patients with urinary bladder cancer. Although sequential multiple hits in the MTS locus were documented in the development of intraurothelial precursor lesions, the MTS genes do not seem to represent a major target for p21-23 deletions in bladder cancer. Two additional tumor suppressor genes involved in bladder neoplasia located distally and proximally to the MTS locus within p22-23 and p11-13 regions respectively were identified. Several distinct putative tumor suppressor gene loci within the q12-13, q21-22, and q34 regions were identified on the q arm. In particular, the pericentromeric q12-13 area may contain the critical tumor suppressor gene or genes for the development of early urothelial neoplasia. Allelic losses of chromosome 9 were associated with expansion of the abnormal urothelial clone which frequently involved large areas of urinary bladder mucosa. These losses could be found in a high proportion of urothelial tumors and in voided urine or bladder washing samples of nearly all patients with urinary bladder carcinoma.  (+info)

Molecular basis for the enterocyte tropism exhibited by Salmonella typhimurium type 1 fimbriae. (2/920)

Salmonella typhimurium exhibits a distinct tropism for mouse enterocytes that is linked to their expression of type 1 fimbriae. The distinct binding traits of Salmonella type 1 fimbriae is also reflected in their binding to selected mannosylated proteins and in their ability to promote secondary bacterial aggregation on enterocyte surfaces. The determinant of binding in Salmonella type 1 fimbriae is a 35-kDa structurally distinct fimbrial subunit, FimHS, because inactivation of fimHS abolished binding activity in the resulting mutant without any apparent effect on fimbrial expression. Surprisingly, when expressed in the absence of other fimbrial components and as a translational fusion protein with MalE, FimHS failed to demonstrate any specific binding tropism and bound equally to all cells and mannosylated proteins tested. To determine if the binding specificity of Salmonella type 1 fimbriae was determined by the fimbrial shaft that is intimately associated with FimHS, we replaced the amino-terminal half of FimHS with the corresponding sequence from Escherichia coli FimH (FimHE) that contains the receptor binding domain of FimHE. The resulting hybrid fimbriae bearing FimHES on a Salmonella fimbrial shaft exhibited binding traits that resembled that of Salmonella rather than E. coli fimbriae. Apparently, the quaternary constraints imposed by the fimbrial shaft on the adhesin determine the distinct binding traits of S. typhimurium type 1 fimbriae.  (+info)

Frequent genetic alterations in simple urothelial hyperplasias of the bladder in patients with papillary urothelial carcinoma. (3/920)

In order to understand the origin of bladder cancer, very early urothelial lesions must be investigated in addition to more advanced tumors. Tissue from 31 biopsies of 12 patients with urothelial hyperplasias and simultaneous or consecutive superficial papillary tumors were used to microdissect urothelium from 15- microm sections of biopsies. The biopsies were obtained with the recently developed highly sensitive diagnostic method of 5-aminolevulinic acid-induced fluorescence endoscopy (AFE). Besides flat and papillary urothelial neoplasms, the method of photodynamic diagnostics also detects simple urothelial hyperplasias as fluorescent positive lesions. In addition, 12 fluorescence-positive biopsies showing histologically normal urothelium were investigated. Fluorescence in situ hybridization was done using a dual color staining technique of biotinylated centromeric probes of chromosomes 9 and 17 and digoxigenin-labeled gene-specific P1 probes for chromosomes 9q22 (FACC), 9p21(p16/CDKI2), and 17p13(p53). Ten of 14 hyperplasias (70%) showed deletions of chromosome 9. In 7 out of 8 patients with genetic alterations in the hyperplasias the genetic change was also present in the papillary tumor. Six out of 12 samples of microdissected normal urothelium also showed genetic alterations on chromosome 9. Microdissection of urothelial lesions, obtained during AFE, has led to the first unequivocal documentation of genetic changes in urothelial lesions diagnosed as normal in histopathology. Thus, this technical approach is important to provide insight into the earliest molecular alterations in bladder carcinogenesis.  (+info)

Urinary bladder transitional cell carcinogenesis is associated with down-regulation of NF1 tumor suppressor gene in vivo and in vitro. (4/920)

The NF1 gene product (neurofibromin) is known to act as a tumor suppressor protein by inactivating ras. The best documented factors involved in urinary bladder transitional cell carcinoma (TCC) are ras proto-oncogene activation and p53 suppressor gene mutations. This is the first study reporting alterations in NF1 gene expression in TCC. We examined NF1 gene expression in a total of 29 surgical urinary bladder TCC specimens representing grades 1 to 3 and in three cell lines, RT4, 5637, and T24 (representing grades 1 to 3, respectively). Decreased NF1 gene expression was observed in 23 of 29 (83%) TCC specimens as estimated by immunohistochemistry, the decrease being more pronounced in high-grade tumors. NF1 mRNA levels were markedly lower in TCC tissue compared with adjacent non-neoplastic urothelium, as studied by in situ hybridization for grade 3 TCC. Immunohistochemistry and Western blotting demonstrated that TCC cell lines expressed NF1 protein at different levels, expression being almost undetectable in T24 (grade 3) cells. Northern blotting for cell lines demonstrated reduced NF1 mRNA levels in grade 3 TCC cells. Reverse transcription polymerase chain reaction for cell lines and selected grade 2 and grade 3 tissue samples demonstrated NF1 type II mRNA isoform predominance in all samples studied. Our results show that both NF1 mRNA and protein levels are decreased in high-grade TCC, suggesting that alterations of NF1 gene expression may be involved in bladder TCC carcinogenesis.  (+info)

Primary uroepithelial cultures. A model system to analyze umbrella cell barrier function. (5/920)

Despite almost 25 years of effort, the development of a highly differentiated and functionally equivalent cell culture model of uroepithelial cells has eluded investigators. We have developed a primary cell culture model of rabbit uroepithelium that consists of an underlying cell layer that interacts with a collagen substratum, an intermediate cell layer, and an upper cell layer of large (25-100 micrometer) superficial cells. When examined at the ultrastructural level, the superficial cells formed junctional complexes and had an asymmetric unit membrane, a hallmark of terminal differentiation in bladder umbrella cells. These cultured "umbrella" cells expressed uroplakins and a 27-kDa uroepithelial specific antigen that assembled into detergent-resistant asymmetric unit membrane particles. The cultures had low diffusive permeabilities for water (2.8 x 10(-4) cm/s) and urea (3.0 x 10(-7) cm/s) and high transepithelial resistance (>8000 Omega cm2) was achieved when 1 mM CaCl2 was included in the culture medium. The cell cultures expressed an amiloride-sensitive sodium transport pathway and increases in apical membrane capacitance were observed when the cultures were osmotically stretched. The described primary rabbit cell culture model mimics many of the characteristics of uroepithelium found in vivo and should serve as a useful tool to explore normal uroepithelial function as well as dysfunction as a result of disease.  (+info)

Proteomics and immunohistochemistry define some of the steps involved in the squamous differentiation of the bladder transitional epithelium: a novel strategy for identifying metaplastic lesions. (6/920)

Here, we present a novel strategy for dissecting some of the steps involved in the squamous differentiation of the bladder urothelium leading to squamous cell carcinomas (SCCs). First, we used proteomic technologies and databases (http://biobase.dk/cgi-bin/celis) to reveal proteins that were expressed specifically by fresh normal urothelium and three SCCs showing no urothelial components. Thereafter, antibodies against some of the differentially expressed proteins as well as a few known keratinocyte markers were used to stain serial cryostat sections (immunowalking) of biopsies obtained from bladder cystectomies of two of the SCC-bearing patients (884-1 and 864-1). Because bladder cancer is a field disease, we surmised that the urothelium of these patients may exhibit a spectrum of abnormalities ranging from early metaplastic stages to invasive disease. Immunohistochemical analysis revealed three types of non-keratinizing metaplastic lesions (types 1-3) that did not express keratins 7, 8, 18, and 20 (expressed by normal urothelium) and could be distinguished based on their staining with keratin 19 antibodies. Type 1 lesions showed staining of all cell layers in the epithelium (with differences in the staining intensity of the basal compartment), whereas type 2 lesions exhibited mainly basal cell staining. Type 3 lesions did not stain with keratin 19 antibodies. In cystectomy 884-1, type 3 lesions exhibited the same immunophenotype as the SCC and may be regarded as precursors to the tumor. Basal cells in these lesions did not express keratin 13, suggesting that the tumor, which was also keratin 13 negative, may have arisen from the expansion of these cells. Similar results were observed with cystectomy 864-1, which showed carcinoma in situ of the SCC type. SCC 864-1 exhibited both keratin 19-negative and -positive cells, implying that the tumor arose from the expansion of the basal cell compartment of type 2 and 3 lesions. Besides providing with a novel strategy for revealing metaplastic lesions, our studies have shown that it is feasible to apply powerful proteomic technologies to the analysis of complex biological samples under conditions that are as close as possible to the in vivo situation.  (+info)

Urothelium-specific expression of an oncogene in transgenic mice induced the formation of carcinoma in situ and invasive transitional cell carcinoma. (7/920)

Although many genetic alterations are known to be associated with human transitional cell carcinoma (TCC) of the urinary bladder, relatively little is known about the roles of these molecular defects, singular or in combination, in bladder tumorigenesis. We have developed a transgenic mouse model of bladder tumorigenesis using a 3.6-kb promoter of uroplakin II gene to drive the urotheliums-specific expression of oncogenes. In this study, we demonstrate that transgenic mice bearing a low copy number of SV40T transgene developed bladder carcinoma in situ (CIS), whereas those bearing high copies developed CIS as well as invasive and metastatic TCCs. These results indicate that the SV40T inactivation of p53 and retinoblastoma gene products, defects frequently found in human bladder CIS and invasive TCCs, can cause the aggressive form of TCC. Our results also provide experimental proof that CIS is a precursor of invasive TCCs, thus supporting the concept of two distinct pathways of bladder tumorigenesis (papillary versus CIS/invasive TCC). This transgenic system can be used for the systematic dissection of the roles of individual or combinations of specific molecular events in bladder tumorigenesis.  (+info)

Specific p53 gene mutations in urinary bladder epithelium after the Chernobyl accident. (8/920)

After the Chernobyl accident, the incidence of urinary bladder cancers in the Ukraine population increased gradually from 26.2 to 36.1 per 100,000 between 1986 and 1996. Urinary bladder epithelium biopsied from 45 male patients with benign prostatic hyperplasia living in radiocontaminated areas of Ukraine demonstrated frequent severe urothelial dysplasia, carcinoma in situ, and a single invasive transitional cell carcinoma, combined with irradiation cystitis in 42 cases (93%). No neoplastic changes (carcinoma in situ or transitional cell carcinoma) were found in 10 patients from clean areas (areas without radiocontamination). DNA was extracted from the altered urothelium of selected paraffin-embedded specimens that showed obviously abnormal histology (3 cases) or intense p53 immunoreactivity (15 cases), and mutational analysis of exons 5-8 of the p53 gene was performed by PCR-single-strand conformational polymorphism analysis followed by DNA sequencing. Nine of 17 patients (53%) had one or more mutations in the altered urothelium. Urine sediment samples were also collected from the patients at 4-27 months after biopsy and analyzed by PCR-single-strand conformational polymorphism analysis or yeast functional assay, and identical or additional p53 mutations were found in four of five cases. Interestingly, a relative hot spot at codon 245 was found in five of nine (56%) cases with mutations, and 11 of the 13 mutations determined (73%) were G:C to A:T transitions at CpG dinucleotides, reported to be relatively infrequent (approximately 18%) in human urinary bladder cancers. Therefore, the frequent and specific p53 mutations found in these male patients may alert us to a future elevated occurrence of urinary bladder cancers in the radiocontaminated areas.  (+info)

Immortalisation of Normal Human Urothelial Cells Compromises Differentiation Capacity. - University of Huddersfield RepositoryImmortalisation of Normal Human Urothelial Cells Compromises Differentiation Capacity. - University of Huddersfield Repository
BACKGROUND: The development of urothelial malignancy is not solely a consequence of loss of proliferation constraints but also involves loss of cellular differentiation, defined histopathologically as grade. Although tumour grade is an independent prognostic marker for urothelial carcinoma (UC), the molecular events underpinning the loss of urothelial differentiation are poorly understood.. OBJECTIVE: To examine the effect of gene alterations implicated in UC development on the ability of human urothelial cells to undergo molecular differentiation and form a functional urothelial barrier.. DESIGN, SETTING, AND PARTICIPANTS: Laboratory study.. INTERVENTION: Normal human urothelial (NHU) cell cultures were transduced with recombinant retroviruses to produce stable sublines overexpressing wild-type or oncogenic mutated fibroblast growth factor receptor 3 or human telomerase reverse transcriptase (hTERT). Previously generated NHU sublines carrying dominant-negative CDK4 and p53 mutant genes or ...
Clinical Trial: Safety And Efficacy Of Js001 For Patients With Locally Advanced Or Metastatic Bladder Urothelial Carcinoma -...Clinical Trial: Safety And Efficacy Of Js001 For Patients With Locally Advanced Or Metastatic Bladder Urothelial Carcinoma -...
U.S., April 14 -- ClinicalTrials.gov registry received information related to the study (NCT03113266) titled Safety and Efficacy of JS001 for Patients With Locally Advanced or Metastatic Bladder Urothelial Carcinoma on April 6. Brief Summary: This is a multi-center, open-label, phase 2 study evaluating the humanized anti-PD-1 antibody JS001, as a monotherapy in patients with locally advanced or metastatic bladder urothelial carcinoma who have failed in routine systemic treatment. Study Start Date: Study Type: Interventional Condition: Bladder Urothelial Carcinoma Intervention: Biological: humanized anti-PD-1 monoclonal antibody humanized anti-PD-1 monoclonal antibody (JS001) is a programmed death-1 (PD-1) immune checkpoint inhibitor antibody, which selectively interferes with the combination of PD-1 with its ligands, PD-L1 and PD-L2, resulting in the activation of lymphocytes and elimination of malignancy theoretically. Other Name: JS001 Recruitment Status: Recruiting Sponsor: Shanghai Junshi ...
British Library EThOS: The regulation of self-renewal in normal human urothelial cellsBritish Library EThOS: The regulation of self-renewal in normal human urothelial cells
The urinary tract is lined by a mitotically-quiescent, but highly regenerative epithelium, the urothelium. The mechanisms regulating urothelial regeneration are incompletely understood although autocrine stimulation of the Epidermal Growth Factor Receptor (EGFR) signalling pathway has been implicated. The hypothesis developed in this thesis is that urothelial homeostasis is regulated through resolution of interactive signal transduction networks downstream of local environmental cues, such as cell:cell contact. Here, canonical Wnt signalling was examined as a candidate key pathway due to the pivotal role of β-catenin in both nuclear transcription and intercellular adherens junctions. Normal human urothelial (NHU) cells isolated from surgical biopsies were grown as finite cell lines in monolayer culture. mRNA analysis from proliferating cultures inferred all components for a functional autocrine-activated canonical Wnt cascade were present. In proliferating cells, β-catenin was nuclear and ...
Squamous differentiation in patients with superficial bladder urothelial carcinoma is associated with high risk of recurrence...Squamous differentiation in patients with superficial bladder urothelial carcinoma is associated with high risk of recurrence...
Prior studies well established that urothelial carcinoma harbors remarkable propensity for divergent differentiation [12-14]. Squamous differentiation has been suggested to be the most common histological variation in bladder urothelial carcinoma and it may present basaloid or clear cell features [15]. However, mechanisms of squamous differentiation in urothelial carcinoma is not very clear. In 1925, Wolbach and Howe found that long time vitamin A deficiency treatment could lead to bladder squamous differentiation in rats [16]. More recently, several findings together with previous studies on the development of squamous differentiation under condition of retinoid deficiency, strongly recommend that retinoid signaling pathways and related networks are significant in the development of squamous lesions in the urinary bladder [17-20]. Maraver et al. showed that downregulation of NOTCH signaling contributed to occurrence of aggression in squamous lesions [21, 22]. Some transcriptional regulators ...
Ketamine-Induced Apoptosis in Normal Human Urothelial Cells - University of Huddersfield RepositoryKetamine-Induced Apoptosis in Normal Human Urothelial Cells - University of Huddersfield Repository
Exposure of urothelium to ketamine resulted in apoptosis, with cytochrome c release from mitochondria and significant subsequent caspase 9 and 3/7 activation. The anaesthetic mode‐of‐action for ketamine is mediated primarily through N‐methyl Daspartate receptor (NMDAR) antagonism; however, NHU cells were unresponsive to NMDAR agonists or antagonists and no expression of NMDAR transcript was detected. Exposure to non‐cytotoxic concentrations of ketamine (≤1 mM) induced rapid release of ATP, which activated purinergic P2Y receptors and stimulated the inositol trisphosphate receptor to provoke transient release of calcium from the endoplasmic reticulum into the cytosol. Ketamine concentrations ,1 mM were cytotoxic and provoked a largeramplitude increase in cytosolic [Ca2+] that was unresolved. The sustained elevation in cytosolic [Ca2+] was associated with pathological mitochondrial oxygen consumption and ATP deficiency ...
Papillary urothelial neoplasm of low malignant potential - WikipediaPapillary urothelial neoplasm of low malignant potential - Wikipedia
In urologic pathology, PUNLMP, short for papillary urothelial neoplasm of low malignant potential, is an exophytic (outward growing), (microscopically) nipple-shaped (or papillary) pre-malignant growth of the lining of the upper genitourinary tract (the urothelium), which includes the renal pelvis, ureters, urinary bladder and part of the urethra. PUNLMP is pronounced pun-lump, like the words pun and lump. As their name suggests, PUNLMPs are neoplasms, i.e. clonal cellular proliferations, that are thought to have a low probability of developing into urothelial cancer, i.e. a malignancy such as bladder cancer. PUNLMPs can lead to blood in the urine (hematuria) or may be asymptomatic. PUNLMPs are exophytic lesions that appear friable to the naked eye and when imaged during cystoscopy. They are definitively diagnosed after removal by microscopic examination by pathologists. Histologically, they have a papillary architecture with slender fibrovascular cores and rare basal mitoses. The papillae ...
Frequent genetic alterations in simple urothelial hyperplasias of the bladder in patients with papillary urothelial carcinoma ...Frequent genetic alterations in simple urothelial hyperplasias of the bladder in patients with papillary urothelial carcinoma ...
In order to understand the origin of bladder cancer, very early urothelial lesions must be investigated in addition to more advanced tumors. Tissue from 31 biopsies of 12 patients with urothelial hyperplasias and simultaneous or consecutive superficial papillary tumors were used to microdissect urothelium from 15- microm sections of biopsies. The biopsies were obtained with the recently developed highly sensitive diagnostic method of 5-aminolevulinic acid-induced fluorescence endoscopy (AFE). Besides flat and papillary urothelial neoplasms, the method of photodynamic diagnostics also detects simple urothelial hyperplasias as fluorescent positive lesions. In addition, 12 fluorescence-positive biopsies showing histologically normal urothelium were investigated. Fluorescence in situ hybridization was done using a dual color staining technique of biotinylated centromeric probes of chromosomes 9 and 17 and digoxigenin-labeled gene-specific P1 probes for chromosomes 9q22 (FACC), 9p21(p16/CDKI2), and ...
JCI - Exosomal long noncoding RNA LNMAT2 promotes lymphatic metastasis in bladder cancerJCI - Exosomal long noncoding RNA LNMAT2 promotes lymphatic metastasis in bladder cancer
Clinical samples and study approval. A total of 266 pairs of tumor tissues and NATs from patients with BCa who underwent surgery was obtained at Sun Yat-sen Memorial Hospital (Cohort 1). Urine and blood samples were obtained from another 206 patients with BCa and 120 healthy participants (Cohort 2). In both cohorts, patients were eligible if they had pathologically confirmed BCa. The clinical features of the patients are summarized in Supplemental Table 1 and Supplemental Table 7. All experiments were conducted with the approval of the Committees for Ethical Review of Research involving Human Subjects at Sun Yat-sen University. Written informed consent was obtained from all participants prior to sample collection.. Cell lines and cell culture. The human BCa cell lines UM-UC-3 (CRL-1749), 5637 (HTB-9), and T24 (HTB-4), and the immortalized normal human urothelial cell line SV-HUC-1 (CRL-9520) were purchased from American Type Culture Collection. UM-UC-3 and T24 cells were cultured in DMEM (Gibco) ...
Vinflunine in routine clinical practice for the treatment of advanced or metastatic urothelial cell carcinoma - data from a...Vinflunine in routine clinical practice for the treatment of advanced or metastatic urothelial cell carcinoma - data from a...
Vinflunine is recommended in the European guideline for the treatment of advanced or metastatic urothelial cell carcinoma (UCC) after failure of platinum-based therapy. This prospective, non-interventional study investigated the safety and efficacy of vinflunine in platinum-pretreated UCC patients in routine clinical practice. Data were prospectively collected on patients with advanced or metastatic UCC undergoing vinflunine treatment in 39 German hospitals and medical practices. Dosing of vinflunine, tumor assessments and concomitant medications followed physicians routine clinical practice. Primary endpoints were toxicity and assessment of vinflunine treatment modalities. Secondary aims included overall response rate (ORR), overall survival (OS) time and a prognostic risk-model. Seventy-seven platinum-pretreated patients were recruited. Vinflunine was predominantly administered as second-line (66 %) therapy or in subsequent treatment lines (21 %). One third of the patients received at least six
Distribution of lymphocytes of the alpha(E)beta(7) phenotype and E-cadherin in normal human urothelium and bladder carcinomas. ...Distribution of lymphocytes of the alpha(E)beta(7) phenotype and E-cadherin in normal human urothelium and bladder carcinomas. ...
The primary aim of this work was to survey normal urothelium and transitional cell carcinoma (TCC) for the presence of T lymphocytes expressing the intraepithelial, CD103(+) phenotype. This antigen defines the alpha(E)beta(7)-integrin. The adhesive counter-receptor for alpha(E)beta(7) is E-cadherin, which is down-regulated during cancer progression. The secondary aim was to determine the pattern of distribution of CD103(+) lymphocytes in relation to E-cadherin expression in bladder cancer. Cryostat sections of normal bladder and TCC were treated with antibodies specific for human CD103, CD3, CD8 and E-cadherin. Visualization was performed by immunoperoxidase or alkaline phosphatase development with light and confocal microscopy. Dual staining and serial sections were used to assess the relationship between these antigens. Four samples of normal bladder and 26 TCC samples were assessed. Occasional T lymphocytes (CD3(+)) were seen in normal urothelium and lamina propria. In the urothelium the majority of
Southgate, Professor Jenny - Biology, The University of YorkSouthgate, Professor Jenny - Biology, The University of York
Professor Jenny Southgate is Director of the Jack Birch Unit for Molecular Carcinogenesis (JBU) and holds a Research Chair funded externally by York Against Cancer. The research of the JBU focuses on human epithelial tissues and their cancers. Our aims are to understand the processes that control proliferation, differentiation and cellular organisation in normal and wounded epithelial tissues and how dysregulation of these processes leads to the development and progression of malignant disease.. Most of the work in the Unit focuses on urothelium, the specialised epithelium that lines the urinary tract and gives rise to bladder cancer. A range of cell and tissue culture systems have been developed to study urothelial cells from normal and diseased tissues, and methods have been established using retroviruses to enable gene manipulation in order to recapitulate the early stages of neoplastic development. The capacity for in vitro-propagated normal human urothelial cells to undergo differentiation ...
E-GEOD-27014 - Kruppel-like factor 5 is Required for Urothelial Maturation - OmicsDIE-GEOD-27014 - Kruppel-like factor 5 is Required for Urothelial Maturation - OmicsDI
Kruppel-like transcription factor 5 (Klf5) is expressed during late embryogenesis in the forming murine bladder urothelium. Targeted disruption of the Klf5flox alleles by the ShhGfpCre transgene resulted in failure of the bladder urothelium to mature accompanied by hydronephrosis, hydroureter, and vesicoureteric reflux in all E18.5 fetuses. The bladder urothelium did not stratify nor did it express terminal differentiation markers characteristic of basal, intermediate, and umbrella cells including keratins 20, 14, and 5, and uroplakins. At E18.5, an ectopic alpha smooth muscle actin positive layer of cells was identified subjacent to the undifferentiated Klf5-deficient urothelium. The effects of Klf5 deficiency were unique to the urothelium since maturation of the epithelium comprising the bladder neck and urethra were unaffected by the lack of KLF5. mRNA microarray analysis of whole E14.5 control and Klf5 deficient bladders identified Ppar and Grhl3 as putative downstream intermediary transcription
Study Validating Ability of microRNAs to Predict Progression of Bladder Cancer Published in British Journal of Urology...Study Validating Ability of microRNAs to Predict Progression of Bladder Cancer Published in British Journal of Urology...
PHILADELPHIA and REHOVOT, Israel, Feb. 7, 2013 /PRNewswire/ -- Rosetta Genomics Ltd. , a leading developer and provider of microRNA-based molecular diagnostics, today announced that data from a study demonstrating the ability of microRNA expression to serve as a biomarker to predict the progression of bladder urothelial carcinoma were published online in the British Journal of Urology International, in an article entitled, Predicting progression of bladder urothelial carcinoma using microRNA
5-HT2A receptor enhancement of contractile activity of the porcine urothelium and lamina propria<...5-HT2A receptor enhancement of contractile activity of the porcine urothelium and lamina propria<...
TY - JOUR. T1 - 5-HT2A receptor enhancement of contractile activity of the porcine urothelium and lamina propria. AU - Moro, Christian. AU - Edwards, Lily. AU - Chess-Williams, Russ. N1 - © 2016 The Japanese Urological Association.. PY - 2016/11. Y1 - 2016/11. N2 - OBJECTIVES: To examine the effect of 5-hydroxytryptamine (5-HT; serotonin) on the contractile properties of the urothelium and lamina propria, as a better understanding of bladder physiology might aid the development of new treatments.METHODS: Strips of porcine urothelium and lamina propria were suspended in gassed Krebs-bicarbonate solution, and cumulative concentration-response curves for 5-HT were generated in the absence and presence of 5-HT antagonists, Nω-nitro-l-arginine and indomethacin. Responses to α-methyl-5-HT were also examined.RESULTS: Strips of urothelium/lamina propria developed spontaneous contractions, whereas the addition of 5-HT induced concentration-dependent increases in contractile tone with maximal ...
Biomaterial comparison for hUCs | Journal of The Royal Society InterfaceBiomaterial comparison for hUCs | Journal of The Royal Society Interface
In this study, we used hUCs taken from the ureter during routine surgery. These cells are fairly similar to cells in the proximal part of the urethra, as the whole urinary tract except the distal part of urethra is covered by the urothelium and therefore these cells can be used in future urethral reconstruction applications [15]. Urothelial cells have also been widely studied in vitro for urothelial tissue engineering purposes; further, Fossum et al. [16] used bladder urothelial cells for clinical urethra reconstruction. Moreover, urethral stratified squamous epithelial cells could not be used for this since it would have been unethical to take tissue from the urethra because the urethra is susceptible to scarring [17].. The identity of hUCs was confirmed using FACS analysis. The markers CD44, CD73, CD105, CD133 and Keratin 8/18 were selected for this study as they have been previously used to characterize hUCs. Our characterization results were consistent with previous results for urothelial ...
College of American Pathologists - December 2001 Anatomic AbstractsCollege of American Pathologists - December 2001 Anatomic Abstracts
Subjective assessment of flat urothelial lesions has often resulted in an under- or overdiagnosis of urothelial carcinoma in situ. To identify reproducible morphologic objective criteria, the authors used an image analysis system to measure several nuclear features (area, diameter, roundness, ellipticity, and optical density) in 20 cases each of CIS, urothelial dysplasia, and normal urothelium with adjacent lymphocyte controls. They did not analyze reactive urothelial lesions and umbrella cells. The most useful, statistically significant morphologic parameter was mean nuclear area of the largest 25 percent of nuclei. The mean upper quartile nuclear area relative to lymphocytes was 2.2 times (range, 1.4 to 2.8 times) in normal urothelium, 2.9 times (range, 1.8 to 3.6 times) in urothelial dysplasia, and 4.9 times (range, 4.0 to 7.6 times) in CIS. The nuclear size measurements for urothelial dysplasia and normal urothelium overlapped, but the separation between CIS and urothelial dysplasia was ...
AMNIOTIC MEMBRANE SCAFFOLD ENRICHED WITH BLADDER FIBROBLASTS PROMOTES ESTABLISHMENT OF HIGHLY DIFFERENTIATED UROTHELIUM - EMC...AMNIOTIC MEMBRANE SCAFFOLD ENRICHED WITH BLADDER FIBROBLASTS PROMOTES ESTABLISHMENT OF HIGHLY DIFFERENTIATED UROTHELIUM - EMC...
Introduction: Human amniotic membrane (AM) is the innermost layer of placenta and is composed of three distinct strata, namely single epithelial layer, a thick basement membrane and avascular stroma. AM has been widely adopted as a biomaterial in tissue engineering and surgical reconstructions, since it promotes wound healing and epithelialization, has low immunogenicity, reduces inflammation and scarring, and was also shown to possess anti-cancer properties (1). We have shown that AM promotes formation of highly differentiated urothelium, in particular when urothelial cells are cultured on AM stromal side (2). Although AM stroma scaffold allows establishment of differentiated urothelium, its orientation probably does not favour AM integration into surrounding tissue, if applied in vivo. Therefore, we established an in vitro AM model which would allow incorporation of fibroblasts into the AM stroma as well as promote urothelial differentiation.. Materials and Methods: Porcine bladder fibroblasts ...
Urothelial Carcinoma Diagnostics Market to Reflect Impressive Growth Rate During 2017 - 2025 - Analytics NewsUrothelial Carcinoma Diagnostics Market to Reflect Impressive Growth Rate During 2017 - 2025 - Analytics News
Persistence Market Research(PMR), in its recent market report, suggests that the Urothelial Carcinoma Diagnostics Market report is set to exceed US$ xx Mn/Bn. The report finds that the Urothelial Carcinoma Diagnostics Market registered ~US$ xx Mn/Bn in 2018 and is spectated to grow at a healthy CAGR over the foreseeable period 2017 - 2025.. The Urothelial Carcinoma Diagnostics Market research focuses on the market structure and various factors (positive and negative) affecting the growth of the market. The study encloses a precise evaluation of the Urothelial Carcinoma Diagnostics Market, including growth rate, current scenario, and volume inflation prospects, on the basis of DROT and Porters Five Forces analyses. In addition, the Urothelial Carcinoma Diagnostics Market study provides reliable and authentic projections regarding the technical jargon.. This Press Release will help you to understand the Volume, growth with Impacting Trends. Click To get SAMPLE PDF (Including Full TOC, Table & ...
2-Methoxyestradiol Induces Mitotic Arrest, Apoptosis, and Synergistic Cytotoxicity with Arsenic Trioxide in Human Urothelial...2-Methoxyestradiol Induces Mitotic Arrest, Apoptosis, and Synergistic Cytotoxicity with Arsenic Trioxide in Human Urothelial...
2-Methoxyestradiol Induces Mitotic Arrest, Apoptosis, and Synergistic Cytotoxicity with Arsenic Trioxide in Human Urothelial Carcinoma Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Long-term low-dose exposure of human urothelial cells to sodium arsenite activates lipocalin-2 via promoter hypomethylation. |...Long-term low-dose exposure of human urothelial cells to sodium arsenite activates lipocalin-2 via promoter hypomethylation. |...
Sigma-Aldrich offers abstracts and full-text articles by [Hsiu-Hua Wang, Meei-Maan Wu, Michael W Y Chan, Yeong-Shiau Pu, Chien-Jen Chen, Te-Chang Lee].
Urothelial cancer of Bladder in y... preview & related info | MendeleyUrothelial cancer of Bladder in y... preview & related info | Mendeley
(2014) Telli et al. Kaohsiung Journal of Medical Sciences. Bladder urothelial carcinoma is rare in young adults and occurs more commonly in older individuals. The aim of this study was to compare the clinical behavior, pathologic characteristics, and prognosis of urothelial carcinoma of urinary b...
Thomas Powles, MD, on Urothelial Carcinoma: Results From the IMvigor211 Trial - The ASCO PostThomas Powles, MD, on Urothelial Carcinoma: Results From the IMvigor211 Trial - The ASCO Post
Thomas Powles, MD, of Barts Cancer Institute, discusses phase III study findings on atezolizumab vs chemotherapy in platinum-treated locally advanced or metastatic urothelial carcinoma, with an emphasis on immune biomarkers, tumor mutational burden, and clinical outcomes (Abstract 409).. ...
S100P as a Marker for Urothelial Histogenesis: A Critical Review and Comparison with Novel and Traditional Urothelial...S100P as a Marker for Urothelial Histogenesis: A Critical Review and Comparison with Novel and Traditional Urothelial...
TY - JOUR. T1 - S100P as a Marker for Urothelial Histogenesis. T2 - A Critical Review and Comparison with Novel and Traditional Urothelial Immunohistochemical Markers. AU - Suryavanshi, Moushumi. AU - Sanz-Ortega, Julian. AU - Sirohi, Deepika. AU - Divatia, Mukul K.. AU - Ohe, Chisato. AU - Zampini, Claudia. AU - Luthringer, Daniel. AU - Smith, Steven C.. AU - Amin, Mahul. PY - 2017/1/1. Y1 - 2017/1/1. N2 - S100P, or placental S100, is a member of a large family of S100 proteins and considered to be a promising immunohistochemical marker to support urothelial differentiation. This review synthesizes published data regarding the expression of S100P in urothelial carcinoma across histological grade and variant patterns, and in other malignancies, in an effort to summarize the state of understanding of this marker and evaluate its potential. We provide also a broad comparison of S100P with other contemporary and traditional urothelial markers and outline the potential utility of S100P in various ...
Treatment Analysis on Urothelial carcinoma Market 2018: Therapeutic Survey and Clinical Management Report 2023 | MedgadgetTreatment Analysis on Urothelial carcinoma Market 2018: Therapeutic Survey and Clinical Management Report 2023 | Medgadget
Urothelial carcinoma Market is also called as transitional cell carcinoma which is a cancerous tumor of the bladder that can spread to other parts of the
A promising target for immunotherapy? | Urology TimesA promising target for immunotherapy? | Urology Times
Among the AUA annual meeting take-home messages in basic science is that the immune co-stimulatory molecule B7-H4 is highly expressed in the luminal subtype of bladder urothelial carcinoma and is associated with poor survival.
Differential Regulation of Growth-Promoting Signalling Pathways by E-Cadherin<...Differential Regulation of Growth-Promoting Signalling Pathways by E-Cadherin<...
TY - JOUR. T1 - Differential Regulation of Growth-Promoting Signalling Pathways by E-Cadherin. AU - Georgopoulos, Nikolaos T.. AU - Kirkwood, Lisa A.. AU - Walker, Dawn C.. AU - Southgate, Jennifer. PY - 2010/10/26. Y1 - 2010/10/26. N2 - Background: Despite the well-documented association between loss of E-cadherin and carcinogenesis, as well as the link between restoration of its expression and suppression of proliferation in carcinoma cells, the ability of E-cadherin to modulate growth-promoting cell signalling in normal epithelial cells is less well understood and frequently contradictory. The potential for E-cadherin to co-ordinate different proliferation-associated signalling pathways has yet to be fully explored.Methodology/Principal Findings: Using a normal human urothelial (NHU) cell culture system and following a calcium-switch approach, we demonstrate that the stability of NHU cell-cell contacts differentially regulates the Epidermal Growth Factor Receptor (EGFR)/Extracellular ...
Molecular subtypes of urothelial carcinoma are defined by specific gene regulatory systems | BMC Medical Genomics | Full TextMolecular subtypes of urothelial carcinoma are defined by specific gene regulatory systems | BMC Medical Genomics | Full Text
Molecular stratification of bladder cancer has revealed gene signatures differentially expressed across tumor subtypes. While these signatures provide important insights into subtype biology, the transcriptional regulation that governs these signatures is not well characterized. In this study, we use publically available ChIP-Seq data on regulatory factor binding in order to link transcription factors to gene signatures defining molecular subtypes of urothelial carcinoma. We identify PPARG and STAT3, as well as ADIRF, a novel regulator of fatty acid metabolism, as putative mediators of the SCC-like phenotype. We link the PLK1-FOXM1 axis to the rapidly proliferating Genomically Unstable and SCC-like subtypes and show that differentiation programs involving PPARG/RXRA, FOXA1/GATA3 and HOXA/HOXB are differentially expressed in UC molecular subtypes. We show that gene signatures and regulatory systems defined in urothelial carcinoma operate in breast cancer in a subtype specific manner, suggesting
Clinical Trials DashboardClinical Trials Dashboard
This is a Phase 3 multicenter, double-blind, randomized, placebo-controlled study to evaluate the efficacy of giving an oral targeted FGFR1-3 inhibitor, infigratinib, as adjuvant treatment following surgery in adult subjects with invasive urothelial carcinoma and susceptible FGFR3 genetic alterations (mutations, and gene fusions or translocations [ie, rearrangements) who have disease that is considered at high risk for recurrence with surgery alone. The study enrolls subjects with either bladder cancer post radical cystectomy or upper tract urothelial cancer post distal ureterectomy and/or nephrectomy. Study treatment is randomized between infigratinib or placebo with treatment until invasive local or distal disease recurrence. ...
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Urothelial carcinomas are frequently multicentric, occurring at multiple separate sites within the urinary tract (4, 5). Urothelial tumor multifocality is often present before the onset of clinical symptoms (3), and patients with multiple, separate tumors are at higher risk for recurrence, progression, and cancer-related death (4, 5, 44). Although numerous studies have been done to address the clonal nature of multifocal urothelial carcinoma, no consensus currently exists on whether these lesions are monoclonal in origin or whether they arise independently. Two explanations have been proposed to explain tumor multifocality within the urothelium: the monoclonal theory and the "field-effect" theory. Whereas the two theories are not mutually exclusive, it is unknown which mechanism is more important in leading to urothelial tumor multifocality. Detailed characterization and comparison of genetic alterations in the cells of separate tumors may provide information about the clonal evolution of ...
bmp10 Protein, BMP10 bone morphogenetic protein 10 - Creative BioMartbmp10 Protein, BMP10 bone morphogenetic protein 10 - Creative BioMart
FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs, FGF10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells.Recombinant FGF10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre-adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.
Papillary urothelial hyperplasia icd 10 Traducere ovarian în românăPapillary urothelial hyperplasia icd 10 Traducere "ovarian" în română
First: Your question is too long to answer here. Dintre toate procedurile, intervenţiile abdominale au cel mai mare impact, fie că este vorba despre laparotomie sau laparoscopie. Prostata este o glandă anexă a aparatului genital masculin, situată în jurul porţiunii iniţiale a uretrei.
Atypical Urothelial Cells Indeterminate for Neoplasia - Urothelial CellsAtypical Urothelial Cells Indeterminate for Neoplasia - Urothelial Cells
Unfortuntely, as in every other body site, cytologic samples from the urinary tract are not always readily placed into distinct categories. An atypical
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3148 Aim: Inverted papilloma of the urinary tract (IP) is thought to be benign, but some urothelial carcinomas show a prominent inverted growth pattern (invUC) which may pose a diagnostic dilemma to the pathologist and clinician. Ancillary markers may help to resolve diagnostically challenging cases and may contribute to the ongoing discussion of a possible malignant potential of some IP. We previously detected a frequent inverted growth pattern in urothelial carcinomas of the ureter and renal pelvis with microsatellite instability and loss of mismatch repair protein expression, raising the possibility that inverted growth of urothelial tumors in general could be associated with a mutator phenotype. The aim of the present study was to characterize the most frequent molecular alterations detected in urothelial carcinomas in a well defined cohort of inverted tumors of the urinary bladder. Methods: Seventy-two cases diagnosed as IP were reviewed by 5 uropathologists. Final diagnosis was IP in 63 ...
Moffitt Cancer Center: Clinical Trial 18509 | MoffittMoffitt Cancer Center: Clinical Trial 18509 | Moffitt
Research Hypothesis: Treatment with nivolumab will extend disease-free survival, compared with placebo, as adjuvant therapy in all randomized patients and in patients with PD-Ll expressing tumors (membranous staining in (great or equal to 1%) with high risk residual disease at radical resection of invasive urothelial carcinoma (IUC). Co-Primary Objectives: To compare the disease free survival (DFS) for nivolumab versus placebo in: Subjects with tumors expressing PD-LI (great or equal to 1% membranous staining in tumor cells) and; All randomized subjects. Secondary Objectives: To compare non-urothelial tract recurrence free survival (NUTRFS) for nivolumab versus placebo in subjects with tumors expressing PD-LI (great or equal to 1% membranous staining in tumor cells) and all randomized subjects. To compare the disease specific survival (DSS) for nivolumab and placebo in subjects with tumors expressing PD-LI (great or equal to 1% membranous staining in tumor cells) and all randomized subjects. To ...
Most recent papers in the journal Analytical and Quantitative Cytopathology and Histopathology | Read by QxMDMost recent papers in the journal Analytical and Quantitative Cytopathology and Histopathology | Read by QxMD
OBJECTIVE: To identify the most useful method to detect the mitotic count (MC) by comparing different techniques, to determine a cutoff value for mitotic activity (MA), and to evaluate the correlation of this value with the recurrence of noninvasive low-grade papillary urothelial neoplasm (LGPUC). STUDY DESIGN: Hematoxylin and eosin-stained slides of 55 LGPUC cases were evaluated for their MA. MC was determined using 4 different methods. In Method 1, cases with 3 mitoses in 1 single focus of a high-power field (HPF x 400) were found, and in Method 2, cases with ≥ 5 mitoses in 1 singlefocus of an HPF, in any level of the neoplastic epithelium, were determined ...
Clinical Trial: NCT03785925 - My Cancer GenomeClinical Trial: NCT03785925 - My Cancer Genome
Inclusion Criteria: - Provide written, informed consent to participate in the study and follow the study procedures - Eastern Cooperative Oncology Group (ECOG) performance status of ≤ 2 - Measurable disease per RECIST 1.1 criteria - Tumor must be PD-L1 low defined by a Combined Positive Score (CPS) of , 10 utilizing the Dako PD-L1 immunohistochemistry (IHC) 22C3 pharmDx assay - Histologically or cytologically documented inoperable, locally advanced or metastatic urothelial cell carcinoma (also termed TCC) - Fresh biopsy or archival tissue - No prior systemic chemotherapy or investigational agent for inoperable locally advanced or mUC - Ineligible for cisplatin Exclusion Criteria: - Patients who have an active, known or suspected autoimmune disease - Patients must not have received prior IL-2 therapy - Prior treatment with an anti PD-1, anti PD-L1, or anti cytotoxic T lymphocyte associated protein 4 (anti CTLA-4) antibody, agents that target IL-2, or any other antibody or drug specifically ...
Development of a Non-Invasive DNA Methylation-Based Assay System for the Risk Assessment of Urothelial CarcinomaDevelopment of a Non-Invasive DNA Methylation-Based Assay System for the Risk Assessment of Urothelial Carcinoma
Bladder cancer ranks the ninth in worldwide cancer incidence. Approximate 90% of bladder cancer is the malignancy of urothelium tissues, the urothelial
Case 34Case 34
Microscopic description: Urothelial carcinoma may show features of low grade or high grade carcinoma (1). This particular FNA from an axillary lymph node shows a monomorphic population of relatively bland appearing epithelioid cells. If not for the scattered mitotic figures and location, it may be difficult at first glance to recognize this specimen as a malignant process. Urothelial carcinomas may appear cohesive, as in this example, or loose to discohesive. High grade urothelial carcinomas are usually more pleomorphic and discohesive. Cercariform cells and eosinophilic bodies (Melamed-Wolinska bodies) may be seen (2). Necrosis is a common occurrence in high grade lesions. Further complicating diagnosis is the occasional presence of squamous differentiation, glandular differentiation, or spindle cells usually seen in high grade lesions. Differential Diagnosis: Clinical history is important to keeping urothelial carcinoma in your differential, particularly in low grade appearing lesions. ...
CiNii Articles - Role of syndecan-1 (CD138) in cell survival of human urothelial carcinomaCiNii Articles - Role of syndecan-1 (CD138) in cell survival of human urothelial carcinoma
KOZAKAI Tomonori , NISHIZAWA Kazuyo , ISHIDA Akiko , IIZUKA Keiji , OTA Hiroyoshi The Journal of the Japanese Society of Clinical Cytology 52(1), 17-22, 2013-01-22 J-STAGE Ichushi Web References (15) ...
https://www.medicalnewstoday.com/articles/319477.phphttps://www.medicalnewstoday.com/articles/319477.php
What is papillary urothelial carcinoma? What are the signs of papillary urothelial carcinoma, what are the causes, and what are the risk factors?
Urothelial carcinoma: Patterns of recurrence | 25th European Symposium on Urogenital Radiology 2018Urothelial carcinoma: Patterns of recurrence | 25th European Symposium on Urogenital Radiology 2018
The main objective of this presentation is to show and evaluate common and uncommon recurrence patterns of urothelial carcinoma (UC) in the urinary tract and elsewhere in the body.. ...
A Study to Evaluate the Efficacy and Safety of Pemigatinib (INCB054828) in Subjects With Urothelial Carcinoma - (FIGHT-201) |...A Study to Evaluate the Efficacy and Safety of Pemigatinib (INCB054828) in Subjects With Urothelial Carcinoma - (FIGHT-201) |...
Clinical trial for Ulcerative Colitis | UC | Ulcerative Colitis (Pediatric) , A Study to Evaluate the Efficacy and Safety of Pemigatinib (INCB054828) in Subjects With Urothelial Carcinoma - (FIGHT-201)
Dr. Petrylak on Combinations with Pembrolizumab in Urothelial CarcinomaDr. Petrylak on Combinations with Pembrolizumab in Urothelial Carcinoma
Daniel P. Petrylak, MD, professor of Medicine and Urology, Yale Cancer Center, discusses combinations with pembrolizumab (Keytruda) for patients with urothelial carcinoma.
New Urothelial carcinoma medicines, recently approved | TheSocialMedworkNew Urothelial carcinoma medicines, recently approved | TheSocialMedwork
You can order the latest approved medicines for Urothelial Carcinoma from TheSocialMedwork if the drugs are not available in your country.
Smoking Ups Risk of Urothelial CarcinomaSmoking Ups Risk of Urothelial Carcinoma
Smoking boosts the risk of developing serious forms of urothelial carcinoma and increased likelihood of dying from the disease, finds study published in BJU International.
Pazopanib and Vinflunine in Urothelial Cancer of the BladderPazopanib and Vinflunine in Urothelial Cancer of the Bladder
Urothelial carcinoma of the bladder mostly is chemically induced and represents the second prevalent urooncological disease. About 20% of newly diagnose
Durvalumab granted Breakthrough Therapy designation by US FDA for treatment of patients with PD-L1 positive urothelial bladder...Durvalumab granted Breakthrough Therapy designation by US FDA for treatment of patients with PD-L1 positive urothelial bladder...
17 February 2016 AstraZeneca and MedImmune, its global biologics research and development arm, today announced that the US Food and Drug Administration (FDA) has granted Breakthrough Therapy...
Ramucirumab Prolongs Progression-free Survival in Platinum-refractory Urothelial Cancer - Cancer Therapy AdvisorRamucirumab Prolongs Progression-free Survival in Platinum-refractory Urothelial Cancer - Cancer Therapy Advisor
Researchers randomly enrolled 530 patients with urothelial carcinoma who had progressed after platinum-based chemotherapy to receive ramucirumab.
2014 October | SYK Pathway2014 October | SYK Pathway
These were able to get followed for recurrence of urothelial cancer from Inhibitors,Modulators,Libraries two months up to 59 months. This permitted an analysis of 18 recurrences and 29 non recur rences in individuals yielding cytologies with MT 3 constructive cells and seven recurrences and 24 non recurrences in individuals yielding cytologies without any MT 3 beneficial cells. A com parison on the time to recurrence involving these two groups unveiled a significant statistical difference in between individuals with urinary cytologies with MT three staining cells and people with no MT 3 staining cells. Discussion The first objective of this review was to determine if epige netic modification was accountable for that silencing from the MT 3 gene within the parental UROtsa cell line. Deal with ment with the parental UROtsa cells with five AZC, a com monly applied agent to find out DNA methylation standing, was shown to possess no effect on MT three mRNA expres sion.. This gives proof the MT three ...
Medicament pentru viermi nemocid - Papilloma urothelial neoplasmMedicament pentru viermi nemocid - Papilloma urothelial neoplasm
Unii oameni cer sfaturi, alții o recomandă, iar alții scriu recenzii, dar absolut toată lumea este interesată de un singur lucru: cum să scapi de paraziți în organism cu medicamente. Larvele helminților au dimensiuni microscopice și nu sunt vizibile pentru ochiul liber.
Papillary urothelial neoplasm of low malignant potentialPapillary urothelial neoplasm of low malignant potential
Urinary systemUrinary system
UPK2UPK2
UPK1BUPK1B
Adrenergic receptorAdrenergic receptor
Transitional epitheliumTransitional epithelium
Beta-2 adrenergic receptorBeta-2 adrenergic receptor
Alpha-1 adrenergic receptorAlpha-1 adrenergic receptor
Beta-1 adrenergic receptorBeta-1 adrenergic receptor
Staphylococcus saprophyticusStaphylococcus saprophyticus
Invasive urothelial carcinomaInvasive urothelial carcinoma
Analgesic nephropathyAnalgesic nephropathy
Fibroblast growth factor receptor 3Fibroblast growth factor receptor 3
ERBB4ERBB4
SerotoninSerotonin
Nitric oxideNitric oxide
Gallium nitrateGallium nitrate
Parasympathetic nervous systemParasympathetic nervous system
Urinary bladderUrinary bladder
Douglas ScherrDouglas Scherr
Interstitial cystitisInterstitial cystitis
Urinary urgencyUrinary urgency
LYPD3LYPD3
Transitional cell carcinomaTransitional cell carcinoma
LY75LY75
UreterUreter
Ureteric balloon catheterUreteric balloon catheter
Sympathetic nervous systemSympathetic nervous system
Neuroendocrine differentiationNeuroendocrine differentiation
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UrotheliumUrinary BladderUroplakin IIUroplakin IIIUrinary Bladder NeoplasmsCarcinoma, Transitional CellUrinary Bladder DiseasesUrologic NeoplasmsCystitisUroplakin IbUrinationUrinary TractUreterCystitis, InterstitialUroplakin IaUrinary Bladder, OveractiveCryoultramicrotomyUrogenital NeoplasmsAdministration, IntravesicalButylhydroxybutylnitrosamineCacodylic AcidPurinergic P2Y Receptor AgonistsUrethraUrodynamicsEpitheliumTetraspaninsKeratins, Type IMuscle, SmoothLewis Blood-Group SystemHyperplasiaKidney PelvisUropathogenic Escherichia coliCystectomyMicroscopy, Electron, ScanningKeratin-13UrineMucous MembraneImmunohistochemistryKeratin-20Urinary Bladder, NeurogenicUrinary Bladder CalculiPapillomaMuscarinic AntagonistsRats, Inbred F344Receptors, Purinergic P2X3ArchivesBiological Science DisciplinesPeriodicals as TopicPubMedDirectories as TopicGingival RecessionGingivoplastyUrinary Tract InfectionsConnective Tissue
KAKEN - Research Projects | Transmitters derived from vesical urothelium ? in association with the pathogenesis ofInterstitial...KAKEN - Research Projects | Transmitters derived from vesical urothelium ? in association with the pathogenesis ofInterstitial...
Program Project: Growth, Differentiation and Disease of Urothelium - Tung-Tien SunProgram Project: Growth, Differentiation and Disease of Urothelium - Tung-Tien Sun
AMNIOTIC MEMBRANE SCAFFOLD ENRICHED WITH BLADDER FIBROBLASTS PROMOTES ESTABLISHMENT OF HIGHLY DIFFERENTIATED UROTHELIUM - EMC...AMNIOTIC MEMBRANE SCAFFOLD ENRICHED WITH BLADDER FIBROBLASTS PROMOTES ESTABLISHMENT OF HIGHLY DIFFERENTIATED UROTHELIUM - EMC...
5-HT2A receptor enhancement of contractile activity of the porcine urothelium and lamina propria<...5-HT2A receptor enhancement of contractile activity of the porcine urothelium and lamina propria<...
Papillary urothelial neoplasm of low malignant potential - WikipediaPapillary urothelial neoplasm of low malignant potential - Wikipedia
Distribution of lymphocytes of the alpha(E)beta(7) phenotype and E-cadherin in normal human urothelium and bladder carcinomas. ...Distribution of lymphocytes of the alpha(E)beta(7) phenotype and E-cadherin in normal human urothelium and bladder carcinomas. ...
Plus itPlus it
College of American Pathologists - December 2001 Anatomic AbstractsCollege of American Pathologists - December 2001 Anatomic Abstracts
British Library EThOS: The regulation of self-renewal in normal human urothelial cellsBritish Library EThOS: The regulation of self-renewal in normal human urothelial cells
Urothelium - WikipediaUrothelium - Wikipedia
Colistin interactions with the mammalian urothelium. - PubMed - NCBIColistin interactions with the mammalian urothelium. - PubMed - NCBI
Kidney, Urothelium - Hyperplasia - Nonneoplastic Lesion AtlasKidney, Urothelium - Hyperplasia - Nonneoplastic Lesion Atlas
In vivo replacement of damaged bladder urothelium by Wolffian duct epithelial cells | PNASIn vivo replacement of damaged bladder urothelium by Wolffian duct epithelial cells | PNAS
Urinary Bladder, Urothelium - Hyperplasia - Gallery - Nonneoplastic Lesion AtlasUrinary Bladder, Urothelium - Hyperplasia - Gallery - Nonneoplastic Lesion Atlas
Combination Chemotherapy Plus Filgrastim in Treating Patients With Locally Recurrent or Advanced Urothelium CancerCombination Chemotherapy Plus Filgrastim in Treating Patients With Locally Recurrent or Advanced Urothelium Cancer
Sorafenib in Treating Patients With Regional or Metastatic Cancer of the UrotheliumSorafenib in Treating Patients With Regional or Metastatic Cancer of the Urothelium
Induction of Human Embryonic and Induced Pluripotent Stem Cells Into Urothelium. | Californias Stem Cell AgencyInduction of Human Embryonic and Induced Pluripotent Stem Cells Into Urothelium. | California's Stem Cell Agency
Mechanisms of Disease: involvement of the urothelium in bladder dysfunctionMechanisms of Disease: involvement of the urothelium in bladder dysfunction
Gemcitabine, Paclitaxel, and Cisplatin in Treating Patients With Advanced Cancer of the UrotheliumGemcitabine, Paclitaxel, and Cisplatin in Treating Patients With Advanced Cancer of the Urothelium
Tissue graft and method for urinary tract urothelium reconstruction and replacement - Patent # 5762966 - PatentGeniusTissue graft and method for urinary tract urothelium reconstruction and replacement - Patent # 5762966 - PatentGenius
Aquaporin water channels in the bladder urothelium as novel targets for treatment of lower urinary tract pathophysiology at...Aquaporin water channels in the bladder urothelium as novel targets for treatment of lower urinary tract pathophysiology at...
Constructs of electrospun PLGA, compressed collagen and minced urothelium for minimally manipulated autologous bladder tissue...Constructs of electrospun PLGA, compressed collagen and minced urothelium for minimally manipulated autologous bladder tissue...
In vitro release of adenosine triphosphate from the urothelium of huma by Vivek Kumar, Christopher R. Chapple et al."In vitro release of adenosine triphosphate from the urothelium of huma" by Vivek Kumar, Christopher R. Chapple et al.
Expression and Localisation of Aquaporin Water Channels in Human Urothelium In Situ and In Vitro - University of Huddersfield...Expression and Localisation of Aquaporin Water Channels in Human Urothelium In Situ and In Vitro - University of Huddersfield...
A phase II trial of gemcitabine plus carboplatin in advanced transitional cell carcinoma of the urothelium | BMC Cancer | Full...A phase II trial of gemcitabine plus carboplatin in advanced transitional cell carcinoma of the urothelium | BMC Cancer | Full...
M-VAC: methotrexate (MTX), vinblastine (VLB), adriamycin (ADM), and cisplatin (DDP) for metastatic and node positive carcinoma...M-VAC: methotrexate (MTX), vinblastine (VLB), adriamycin (ADM), and cisplatin (DDP) for metastatic and node positive carcinoma...
Urothelium | Dominion Internal MedicineUrothelium | Dominion Internal Medicine
Substance P induces localization of MIF/α1-inhibitor-3 complexes to umbrella cells via paracellular transit through the...Substance P induces localization of MIF/α1-inhibitor-3 complexes to umbrella cells via paracellular transit through the...
Frontiers | Increased Expression of Interleukin-6 Family Members and Receptors in Urinary Bladder with Cyclophosphamide-Induced...Frontiers | Increased Expression of Interleukin-6 Family Members and Receptors in Urinary Bladder with Cyclophosphamide-Induced...
Inorganic arsenic-induced intramitochondrial granules in mouse urothelium<...Inorganic arsenic-induced intramitochondrial granules in mouse urothelium<...
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Methotrexate, vinblastine, doxorubicin, and cisplatin for advanced transitional cell carcinoma of the urothelium. Efficacy and...Methotrexate, vinblastine, doxorubicin, and cisplatin for advanced transitional cell carcinoma of the urothelium. Efficacy and...
Urinary system - WikipediaUrinary system - Wikipedia
In vitro differentiation and propagation of urothelium from pluripotent stem cell lines<...In vitro differentiation and propagation of urothelium from pluripotent stem cell lines<...
Molecular and cellular medicine - York Biomedical Research Institute, University of YorkMolecular and cellular medicine - York Biomedical Research Institute, University of York
Evaluation of Diet and Dimethylarsinic Acid on the Urothelium of Syrian Golden Hamsters<...Evaluation of Diet and Dimethylarsinic Acid on the Urothelium of Syrian Golden Hamsters<...
AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsHumanitiesInformation Science
UrotheliumUrinary BladderUroplakin IIUroplakin IIIUrinary Bladder NeoplasmsCarcinoma, Transitional CellUrinary Bladder DiseasesUrologic NeoplasmsCystitisUroplakin IbUrinationUrinary TractUreterCystitis, InterstitialUroplakin IaUrinary Bladder, OveractiveCryoultramicrotomyUrogenital NeoplasmsAdministration, IntravesicalButylhydroxybutylnitrosamineCacodylic AcidPurinergic P2Y Receptor AgonistsUrethraUrodynamicsEpitheliumTetraspaninsKeratins, Type IMuscle, SmoothLewis Blood-Group SystemHyperplasiaKidney PelvisUropathogenic Escherichia coliCystectomyMicroscopy, Electron, ScanningKeratin-13UrineMucous MembraneImmunohistochemistryKeratin-20Urinary Bladder, NeurogenicUrinary Bladder CalculiPapillomaMuscarinic AntagonistsRats, Inbred F344Receptors, Purinergic P2X3ArchivesBiological Science DisciplinesPeriodicals as TopicPubMedDirectories as TopicGingival RecessionGingivoplastyUrinary Tract InfectionsConnective Tissue
RenalLamina propriaEpitheliumHistologicallyCarcinomaNative urotheliumUrineVitroDifferentiation markersBiopsiesPorcineAllelesTissueNormalSmooth muscleCellsRenal pelvisInjury to the urotheliumDetrusorEpithelialTissueCarcinomasMouse urotheliumNormal urotheliumEntire urotheliumGenitourinaryLesionsApical surfaceHyperplasiaHESCsMetaplasiaAfferentBiopsyAquaporinPathophysiologyCarcinogenesisConclusionsExpressesLymphocytesMalignantBarrierTumourRats
Renal1
  • In urologic pathology, PUNLMP, short for papillary urothelial neoplasm of low malignant potential, is an exophytic (outward growing), (microscopically) nipple-shaped (or papillary) pre-malignant growth of the lining of the upper genitourinary tract (the urothelium), which includes the renal pelvis, ureters, urinary bladder and part of the urethra. (wikipedia.org)
Lamina propria8
  • serotonin) on the contractile properties of the urothelium and lamina propria, as a better understanding of bladder physiology might aid the development of new treatments. (edu.au)
  • METHODS: Strips of porcine urothelium and lamina propria were suspended in gassed Krebs-bicarbonate solution, and cumulative concentration-response curves for 5-HT were generated in the absence and presence of 5-HT antagonists, Nω-nitro-l-arginine and indomethacin. (edu.au)
  • RESULTS: Strips of urothelium/lamina propria developed spontaneous contractions, whereas the addition of 5-HT induced concentration-dependent increases in contractile tone with maximal contractions of 50.43 ± 2.78 mN/g tissue weight (n = 100). (edu.au)
  • CONCLUSIONS: The results show that contractile responses of the urothelium/lamina propria to 5-HT are predominantly mediated through the 5-HT2A receptor. (edu.au)
  • serotonin) on the contractile properties of the urothelium and lamina propria, as a better understanding of bladder physiology might aid the development of new treatments.METHODS: Strips of porcine urothelium and lamina propria were suspended in gassed Krebs-bicarbonate solution, and cumulative concentration-response curves for 5-HT were generated in the absence and presence of 5-HT antagonists, Nω-nitro-l-arginine and indomethacin. (edu.au)
  • Responses to α-methyl-5-HT were also examined.RESULTS: Strips of urothelium/lamina propria developed spontaneous contractions, whereas the addition of 5-HT induced concentration-dependent increases in contractile tone with maximal contractions of 50.43 ± 2.78 mN/g tissue weight (n = 100). (edu.au)
  • However, the 5-HT2A antagonist, ketanserin (30-300 μmol/L), caused a shift of the 5-HT curve yielding an affinity estimate of 7.9.CONCLUSIONS: The results show that contractile responses of the urothelium/lamina propria to 5-HT are predominantly mediated through the 5-HT2A receptor. (edu.au)
  • Occasional T lymphocytes (CD3(+)) were seen in normal urothelium and lamina propria. (ox.ac.uk)
Epithelium2
Histologically1
Carcinoma1
Native urothelium1
  • Amniotic membrane scaffolds enable the development of tissue-engineered urothelium with molecular and ultrastructural properties comparable to that of native urothelium. (emc-proceedings.com)
Urine1
  • To achieve this purpose, we've focused on the transmitters expressed on urothelium or secreted into urine of human IC patients. (nii.ac.jp)
Vitro1
  • With reference to detached matrix-free controls from standard plastic culture (left column) in vitro generated urothelium of PKH26-labelled HUC seeded in high-density on CCC (right column) showed comparable expression pattern of p63, K-20 (both: ongoing differentiation), E-cadherin and ZO-1 (both: junction formation). (omicsonline.org)
Differentiation markers1
Biopsies1
  • Tissue from 31 biopsies of 12 patients with urothelial hyperplasias and simultaneous or consecutive superficial papillary tumors were used to microdissect urothelium from 15- microm sections of biopsies. (uni-regensburg.de)
Porcine1
  • Porcine urothelial cells cultured on de-epithelized AM scaffolds enriched with porcine bladder fibroblasts (A) established highly differentiated urothelium, whereas those maintained on AM scaffolds without integrated fibroblast (B) differentiated partially. (emc-proceedings.com)
Alleles1
  • Targeted disruption of the Klf5flox alleles by the ShhGfpCre transgene resulted in failure of the bladder urothelium to mature accompanied by hydronephrosis, hydroureter, and vesicoureteric reflux in all E18.5 fetuses. (omicsdi.org)
Tissue1
  • Although AM stroma scaffold allows establishment of differentiated urothelium, its orientation probably does not favour AM integration into surrounding tissue, if applied in vivo . (emc-proceedings.com)
Normal3
Smooth muscle1
  • At E18.5, an ectopic alpha smooth muscle actin positive layer of cells was identified subjacent to the undifferentiated Klf5-deficient urothelium. (omicsdi.org)
Cells1
Renal pelvis3
Injury to the urothelium2
Detrusor6
  • Objective - In this set of experiments, we have assessed the contribution of the urothelium to purinergic activity by quantifying the amount of adenosine triphosphate (ATP) released from the urothelium of patients with idiopathic detrusor overactivity (IDO) and with neurogenic detrusor overactivity (NDO) and comparing these releases to those of controls. (edu.au)
  • Q-PCR analyses revealed significant ( p ≤ 0.01) CYP duration- and tissue- (e.g., urothelium, detrusor) dependent increases in LIF, IL-6, IL-6Rα, LIFR, and gp130 mRNA expression. (frontiersin.org)
  • CYP-induced cystitis significantly ( p ≤ 0.01) increased LIF-immunoreactivity (IR) in urothelium, detrusor, and suburothelial plexus whereas increased gp130-IR was only observed in urothelium and detrusor. (frontiersin.org)
  • Expression of these adrenoceptors in surgically separated human urothelium and detrusor muscle were investigated using RT-PCR. (bvsalud.org)
  • The results confirmed the presence of mRNA for ß1, ß2 and ß3-adrenoceptor in both human urothelium and detrusor. (bvsalud.org)
  • Existence of ß3-adrenoceptor mRNA exists in the urothelium in addition to the detrusor muscle suggest multiple site of actions for the ß3-adrenoceptor in the lower urinary tract . (bvsalud.org)
Epithelial3
  • The capacity of Wolffian duct epithelial cells to replace damaged urothelium can potentially be leveraged for bladder replacement therapies which are often necessitated in patients with carcinoma or neurogenic bladder. (pnas.org)
  • Alteration of the epithelial and mesenchymal cell signaling contribution through noncell contact coculture or conditioned media did not enhance the production of urothelium. (ca.gov)
  • The trigone is the triangular area of the bladder bound by the ureteral orifices and the internal urethral sphincter, which is normally lined by urothelium, a type of transitional epithelial tissue. (medscape.com)
Tissue8
  • Although the urothelium maintains a tight barrier to ion and solute flux, a number of local factors (e.g. tissue pH, mechanical or chemical trauma, and bacterial infection) can modulate the barrier function of the urothelium. (pubmedcentralcanada.ca)
  • Cells from minced tissue migrated, expanded and re-organised to a confluent cell layer on the top of the construct after two weeks and formed a multilayered urothelium after four weeks. (diva-portal.org)
  • To investigate the expression of AQP water channels by human urothelium in situ, in proliferating urothelial cell cultures and in differentiated tissue constructs. (hud.ac.uk)
  • Although AM stroma scaffold allows establishment of differentiated urothelium, its orientation probably does not favour AM integration into surrounding tissue, if applied in vivo . (emc-proceedings.com)
  • Amniotic membrane scaffolds enable the development of tissue-engineered urothelium with molecular and ultrastructural properties comparable to that of native urothelium. (emc-proceedings.com)
  • Twenty-six samples of normal control urothelium obtained from patients without urothelial carcinomas (C), 47 samples of non-cancerous urothelium without noticeable morphological changes obtained from patients with urothelial carcinomas (N), and 46 samples of the corresponding cancerous tissue (T) in the learning cohort and 64 N samples in the validation cohort, i.e. 183 tissue samples in total, were analyzed. (elsevier.com)
  • [ 7 , 8 ] Kvist et al did not find estrogen receptors in 36 historically collected samples of squamous metaplasia of the bladder urothelium, although the authors posited that relatively few existing receptors might have been destroyed in the tissue preparation process. (medscape.com)
  • A layer of tissue and blood vessels surrounding the urothelium. (cancersa.org.au)
Carcinomas2
Mouse urothelium1
Normal urothelium2
Entire urothelium1
  • Although the entire urothelium is at risk of urotoxicity, the bladder, which serves as a reservoir, is most frequently affected, because the contact time between acrolein and the urothelium is greatest at this site. (medscape.com)
Genitourinary1
  • The Northwestern Memorial Hospital (Chicago, IL) pathology database was searched for random, nonconsecutive patients over the period of April 2014 to April 2015 who had a biopsy- or surgical-resection diagnosis by a genitourinary specialty pathologist (X.Y.) of reactive urothelium, low grade UC (LG UC) and high grade UC (HG UC) and urothelial CIS (WHO, 2004). (biomedcentral.com)
Lesions3
Apical surface2
Hyperplasia1
HESCs1
  • The protocol can be used to derive urothelium from other hESCs or human-induced pluripotent stem cells. (elsevier.com)
Metaplasia1
Afferent1
Biopsy3
Aquaporin1
Pathophysiology1
Carcinogenesis1
  • High similarities were observed between the rodent urothelium carcinogenesis process and the corresponding process in humans, in regards to the histopathological features and biological alteration profile: DNA aneuploidy, p53 overexpression and high proliferative index measured by Ki-67 immunoexpression. (spandidos-publications.com)
Conclusions2
  • Conclusions - These experiments suggested a significant rise in ATP release from the urothelium of bladders with NDO as well as those with IDO in comparison to controls. (edu.au)
  • Conclusions: This is the first study to demonstrate that AQPs are expressed by human urothelium, suggesting a potential role in transurothelial water and solute transport. (hud.ac.uk)
Expresses1
Lymphocytes1
  • In the urothelium the majority of these T lymphocytes (71%) were also CD8(+) and of these 68% expressed the CD103 marker. (ox.ac.uk)
Malignant1
  • One theory suggests that the multiple tumors are of monoclonal origin, arising from a single malignant transformed cell which proliferates and spreads throughout the urothelium either by intraluminal spread with secondary implantation at different sites within the urinary tract or by intraepithelial migration. (aacrjournals.org)
Barrier5
  • Research efforts into IC are focused on the urothelium, including newly discovered signaling molecules which suggest that the urothelium is far more than a barrier, as well as how the urothelium interacts with proximal nerves and smooth muscle. (wikipedia.org)
  • Although the urinary bladder urothelium has classically been thought of as a passive barrier to ions and solutes, a number of novel properties have been recently attributed to urothelial cells. (pubmedcentralcanada.ca)
  • 1 , 6 , 7 The barrier function of the urothelium is dependent on several features of the umbrella cell layer: these features include tight-junction complexes that reduce the movement of ions and solutes between cells, and specialized lipid molecules and uroplakin proteins in the apical membrane, which reduce the permeability of the cells to small molecules (e.g. water, urea, protons). (pubmedcentralcanada.ca)
  • 11 For example, when protamine sulfate was used to damage selectively only the umbrella cell layer, it was shown that the urothelium rapidly undergoes both functional and structural changes in order to restore the barrier. (pubmedcentralcanada.ca)
  • The urothelium is no longer regarded as a passive barrier but rather as a complex "sensory web" transducing signals from inside the viscus (and in response to neuronal and local changes) through the activation of cell-surface receptors [ 1 ]. (hindawi.com)
Tumour1
Rats2
  • A1-I3 immunostaining was observed in interstitial spaces throughout the bladder (including submucosa) but not urothelium in intact and saline-treated rats. (biomedcentral.com)
  • In SP-treated rats, A1-I3 in the bladder increased and A1-I3 was observed traversing through the urothelium. (biomedcentral.com)
College of American Pathologists - December 2001 Anatomic Abstracts
College of American Pathologists - December 2001 Anatomic Abstracts (cap.org)
Frequent genetic alterations in simple urothelial hyperplasias of the bladder in patients with papillary urothelial carcinoma ...
Frequent genetic alterations in simple urothelial hyperplasias of the bladder in patients with papillary urothelial carcinoma ... (epub.uni-regensburg.de)
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In vivo replacement of damaged bladder urothelium by Wolffian duct epithelial cells | PNAS
In vivo replacement of damaged bladder urothelium by Wolffian duct epithelial cells | PNAS (pnas.org)
Pathogens | Free Full-Text | Histone Deacetylase 6 Regulates Bladder Architecture and Host Susceptibility to Uropathogenic...
Pathogens | Free Full-Text | Histone Deacetylase 6 Regulates Bladder Architecture and Host Susceptibility to Uropathogenic... (mdpi.com)
2017 Grant Recipients | Greenberg Bladder Cancer Institute
2017 Grant Recipients | Greenberg Bladder Cancer Institute (hopkinsmedicine.org)
Clinical Trial With Vinflunine as Maintenance Therapy in Metastatic Urothelial Cancer - Full Text View - ClinicalTrials.gov
Clinical Trial With Vinflunine as Maintenance Therapy in Metastatic Urothelial Cancer - Full Text View - ClinicalTrials.gov (clinicaltrials.gov)
Tissue Engineering for Human Urethral Reconstruction: Systematic Review of Recent Literature
Tissue Engineering for Human Urethral Reconstruction: Systematic Review of Recent Literature (journals.plos.org)
Chemoprevention of Urothelial Cell Carcinoma Growth and Invasion by the Dual COX-LOX Inhibitor Licofelone in UPII-SV40T...
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Permeability Barrier - Methods and Protocols | Kursad Turksen | Springer
Permeability Barrier - Methods and Protocols | Kursad Turksen | Springer (springer.com)
Severity illness scoring systems for early identification and prediction of in-hospital mortality in patients with suspected...
Severity illness scoring systems for early identification and prediction of in-hospital mortality in patients with suspected... (springermedizin.at)
LRP1B | Cancer Genetics Web
LRP1B | Cancer Genetics Web (cancerindex.org)
Urinary system - Wikipedia
Urinary system - Wikipedia (en.m.wikipedia.org)
Runner's Bladder: Exercise-Induced Hematuria With Lower Urinary Tract Pathology
Runner's Bladder: Exercise-Induced Hematuria With Lower Urinary Tract Pathology (urotoday.com)
A Study to Identify Participants With Urothelial Cancer and Fibroblast Growth Factor Receptor Gene Aberrations | Clinical...
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Deletions of chromosome 8p and loss of sFRP1 expression are progression markers of papillary bladder cancer | Laboratory...
Deletions of chromosome 8p and loss of sFRP1 expression are progression markers of papillary bladder cancer | Laboratory... (nature.com)
Anti-BMP4 antibody (ab39973) | Abcam
Anti-BMP4 antibody (ab39973) | Abcam (abcam.com)
Trigonitis: Practice Essentials, Pathophysiology, Epidemiology
Trigonitis: Practice Essentials, Pathophysiology, Epidemiology (emedicine.medscape.com)
Siam Oottamasathien Laboratory - Massachusetts General Hospital, Boston, MA
Siam Oottamasathien Laboratory - Massachusetts General Hospital, Boston, MA (massgeneral.org)
'down regulation' Protocols and Video...
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Hemorrhagic Cystitis: Practice Essentials, Anatomy, Pathophysiology
Hemorrhagic Cystitis: Practice Essentials, Anatomy, Pathophysiology (emedicine.medscape.com)
Histology Of The Urinary Tract Flashcards by Elizabeth Stevens | Brainscape
Histology Of The Urinary Tract Flashcards by Elizabeth Stevens | Brainscape (brainscape.com)
Nested Variant of Urothelial Carcinoma
Nested Variant of Urothelial Carcinoma (hindawi.com)
Plus it
Plus it (cancerres.aacrjournals.org)
Urinary System Flashcards by | Brainscape
Urinary System Flashcards by | Brainscape (brainscape.com)
Apoptosis | PNAS
Apoptosis | PNAS (pnas.org)
Interstitial cystitis and systemic autoimmune diseases | Nature Reviews Urology
Interstitial cystitis and systemic autoimmune diseases | Nature Reviews Urology (nature.com)
Referral Forms & Guidelines
Referral Forms & Guidelines (cancercare.mb.ca)
Pathophysiology of Overactive Bladder | SpringerLink
Pathophysiology of Overactive Bladder | SpringerLink (link.springer.com)
Recombinant Anti-LYPD3 antibody [SP250] - N-terminal (ab192845) | Abcam
Recombinant Anti-LYPD3 antibody [SP250] - N-terminal (ab192845) | Abcam (abcam.com)
Plus it
Plus it (molpharm.aspetjournals.org)