OrganismsDiseasesChemicals and Drugs
UreaseCanavaliaHelicobacter pyloriNickelUreaHelicobacter InfectionsProteus mirabilisEnterobacter aerogenesHelicobacterBacterial ProteinsProteusHelicobacter hepaticus
Urease
An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.
Canavalia
A plant genus of the family FABACEAE. Canavalia ensiformis is the source of CONCANAVALIN A.
Helicobacter pylori
A spiral bacterium active as a human gastric pathogen. It is a gram-negative, urease-positive, curved or slightly spiral organism initially isolated in 1982 from patients with lesions of gastritis or peptic ulcers in Western Australia. Helicobacter pylori was originally classified in the genus CAMPYLOBACTER, but RNA sequencing, cellular fatty acid profiles, growth patterns, and other taxonomic characteristics indicate that the micro-organism should be included in the genus HELICOBACTER. It has been officially transferred to Helicobacter gen. nov. (see Int J Syst Bacteriol 1989 Oct;39(4):297-405).
Nickel
A trace element with the atomic symbol Ni, atomic number 28, and atomic weight 58.69. It is a cofactor of the enzyme UREASE.
Urea
A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.
Helicobacter Infections
Infections with organisms of the genus HELICOBACTER, particularly, in humans, HELICOBACTER PYLORI. The clinical manifestations are focused in the stomach, usually the gastric mucosa and antrum, and the upper duodenum. This infection plays a major role in the pathogenesis of type B gastritis and peptic ulcer disease.
Proteus mirabilis
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that is frequently isolated from clinical specimens. Its most common site of infection is the urinary tract.
Enterobacter aerogenes
Gram-negative, capsulated, gas-producing rods found widely in nature. Both motile and non-motile strains exist. The species is closely related to KLEBSIELLA PNEUMONIAE and is frequently associated with nosocomial infections
Helicobacter
A genus of gram-negative, spiral-shaped bacteria that has been isolated from the intestinal tract of mammals, including humans. It has been associated with PEPTIC ULCER.
Bacterial Proteins
Proteins found in any species of bacterium.
Proteus
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs in the intestines of humans and a wide variety of animals, as well as in manure, soil, and polluted waters. Its species are pathogenic, causing urinary tract infections and are also considered secondary invaders, causing septic lesions at other sites of the body.
Helicobacter hepaticus
A species of HELICOBACTER that colonizes the CECUM and COLON of several strains of MICE, and is associated with HEPATITIS and carcinogenesis.

The marine cyanobacterium Synechococcus sp. WH7805 requires urease (urea amidohydrolase, EC 3.5.1.5) to utilize urea as a nitrogen source: molecular-genetic and biochemical analysis of the enzyme. (1/970)

Cyanobacteria assigned to the genus Synechococcus are an important component of oligotrophic marine ecosystems, where their growth may be constrained by low availability of fixed nitrogen. Urea appears to be a major nitrogen resource in the sea, but little molecular information exists about its utilization by marine organisms, including Synechococcus. Oligonucleotide primers were used to amplify a conserved fragment of the urease (urea amidohydrolase, EC 3.5.1.5) coding region from cyanobacteria. A 5.7 kbp region of the genome of the unicellular marine cyanobacterium Synechococcus sp. strain WH7805 was then cloned, and genes encoding three urease structural subunits and four urease accessory proteins were sequenced and identified by homology. The WH7805 urease had a predicted subunit composition typical of bacterial ureases, but the organization of the WH7805 urease genes was unique. Biochemical characteristics of the WH7805 urease enzyme were consistent with the predictions of the sequence data. Physiological data and sequence analysis both suggested that the urease operon may be nitrogen-regulated by the ntcA system in WH7805. Inactivation of the large subunit of urease, ureC, prevented WH7805 and Synechococcus WH8102 from growing on urea, demonstrating that the urease genes cloned are essential to the ability of these cyanobacteria to utilize urea as a nitrogen source.  (+info)

Role of apoptosis induced by Helicobacter pylori infection in the development of duodenal ulcer. (2/970)

BACKGROUND: Helicobacter pylori affects gastric epithelium integrity by acceleration of apoptosis. However, it remains unclear what product of the bacteria causes apoptosis, or whether or not the apoptosis is involved in the development of ulcers. AIMS: To elucidate the factor from H pylori that causes acceleration of apoptosis and the role of apoptosis in the development of duodenal ulcer in H pylori infection. PATIENTS: Five H pylori negative healthy volunteers, 47 H pylori positive patients with duodenal ulcer, and 35 H pylori positive patients with gastric ulcer. METHODS: An endoscopic examination was carried out to diagnose ulcers and determine their clinical stage. To analyse apoptosis, a cell cycle analysis was performed using biopsy specimens. RESULTS: There was a significant correlation between the urease activity of the H pylori strain and the level of apoptosis induced by this bacterial strain. Moreover, in duodenal ulcer patients infected with H pylori, the patients with an active ulcer exhibited a significantly higher level of apoptosis than those with ulcers at both the healing and scarring stages. CONCLUSION: These findings suggest that acceleration of apoptosis in the antral mucosa caused by the urease of H pylori plays a crucial role in the development of ulcers in the duodenum.  (+info)

A novel urease-negative Helicobacter species associated with colitis and typhlitis in IL-10-deficient mice. (3/970)

A spiral-shaped bacterium with bipolar, single-sheathed flagella was isolated from the intestines of IL-10 (interleukin-10)-deficient (IL-10(-/-)) mice with inflammatory bowel disease. The organism was microaerobic, grew at 37 and 42 degrees C, and was oxidase and catalase positive but urease negative. On the basis of 16S rRNA gene sequence analysis and biochemical and phenotypic criteria, the organism is classified as a novel helicobacter. Cesarean section-rederived IL-10(-/-) mice without helicobacter infection did not have histological evidence of intestinal inflammation. However, helicobacter-free IL-10(-/-), SCID/NCr, and A/JNCr mice experimentally inoculated with the novel urease-negative Helicobacter sp. developed variable degrees of inflammation in the lower intestine, and in immunocompetent mice, the experimental infection was accompanied by a corresponding elevated immunoglobulin G antibody response to the novel Helicobacter sp. antigen. These data support other recent studies which demonstrate that multiple Helicobacter spp. in both naturally and experimentally infected mice can induce inflammatory bowel disease. The mouse model of helicobacter-associated intestinal inflammation should prove valuable in understanding how specific microbial antigens influence a complex disease process.  (+info)

Is routine histological evaluation an accurate test for Helicobacter pylori infection? (4/970)

AIM: To compare the diagnostic accuracy of routine histology for Helicobacter pylori infection, with histology by an expert pathologist, and to compare histology with the rapid urease test (RUT), 13C-urea breath test, IgG serology and culture of antrum and corpus specimens, in a consecutive series of untreated patients presenting for upper oesophago-gastro-duodenoscopy. MATERIALS AND METHODS: One-hundred and fifteen consecutive patients underwent multiple tests for H. pylori infection: rapid urease test, 13C-urea breath test, IgG serology and histology and culture on antrum and corpus biopsy specimens. Histology was first evaluated by the pathologists in a routine examination, and then blindly reviewed by an expert pathologist with a special interest in gastrointestinal pathology. The patients were considered to be H. pylori-positive if two or more tests were positive. RESULTS: Eighty-one patients (70.4%) were found to be H. pylori positive. 13C-urea breath test and IgG serology showed the best sensitivity and specificity (100%). Both the antral and body cultures, and the rapid urease test had the highest specificity (100%). Histological diagnosis after re-evaluation by an expert pathologist showed a high sensitivity (98. 8%) and specificity (100%), and was better than routine histology (sensitivity 92.6%; specificity 90.3%). The accuracy of the rapid urease test was greater than that of routine histology, and the combination of these two tests improved the sensitivity of H. pylori detection to up to 100%. CONCLUSION: All diagnostic tests usually utilised in clinical practice have a sensitivity higher than 90%. In patients who were not pre-treated with antisecretory agents or antibiotics, the sensitivity of histological diagnosis, however, seems to be influenced by the accuracy of the histological examination. The sensitivity of routine histology, but not of revised histological diagnosis, is improved by an additional rapid urease test.  (+info)

Systemic and local immune responses against Helicobacter pylori urease in patients with chronic gastritis: distinct IgA and IgG productive sites. (5/970)

BACKGROUND: Helicobacter pylori urease is a major target for immune responses among various bacterial components in H pylori infected patients. AIMS: To analyse the relation between systemic and local humoral immune responses to H pylori urease and grades of chronic gastritis. PATIENTS: Seventy five patients with chronic gastritis associated with H pylori infection were classified into three groups (grade I, superficial gastritis; II, atrophic gastritis, quiescent; or III, atrophic gastritis, active). METHODS: Anti-H pylori urease specific antibodies in the serum, gastric juice, and biopsy specimens were determined by ELISA or western blotting analysis. The sites for H pylori urease and its specific antibody producing B lymphocytes were confirmed by immunohistochemical analysis. RESULTS: In the sera of patients with grade I gastritis, weak IgG but relatively strong IgG responses to H pylori urease were observed; dominant strong IgG responses were detected in grade II gastritis. In grade III gastritis, significant IgG and IgA responses were obtained. A similar pattern of IgA and IgG responses was detected in gastric juice and tissue. H pylori urease specific, antibody producing B cells were not found in the gastric mucosa of patients with grade I gastritis despite the presence of such B cells in the duodenal bulb. Specific B cells were observed in the gastric mucosa of patients with grade II and III gastritis with atrophy. CONCLUSIONS: Purified H pylori urease, together with localisation of its specific antibody producing B cells, are useful for serological testing and histopathological analysis for determining the stage of chronic gastritis and studying the pathogenesis of H pylori infection.  (+info)

Isolation of Helicobacter pylori genes that modulate urease activity. (6/970)

Helicobacter pylori urease, a nickel-requiring metalloenzyme, hydrolyzes urea to NH3 and CO2. We sought to identify H. pylori genes that modulate urease activity by constructing pHP8080, a plasmid which encodes both H. pylori urease and the NixA nickel transporter. Escherichia coli SE5000 and DH5alpha transformed with pHP8080 resulted in a high-level urease producer and a low-level urease producer, respectively. An H. pylori DNA library was cotransformed into SE5000 (pHP8080) and DH5alpha (pHP8080) and was screened for cotransformants expressing either lowered or heightened urease activity, respectively. Among the clones carrying urease-enhancing factors, 21 of 23 contained hp0548, a gene that potentially encodes a DNA helicase found within the cag pathogenicity island, and hp0511, a gene that potentially encodes a lipoprotein. Each of these genes, when subcloned, conferred a urease-enhancing activity in E. coli (pHP8080) compared with the vector control. Among clones carrying urease-decreasing factors, 11 of 13 clones contained the flbA (also known as flhA) flagellar biosynthesis/regulatory gene (hp1041), an lcrD homolog. The LcrD protein family is involved in type III secretion and flagellar secretion in pathogenic bacteria. Almost no urease activity was detected in E. coli (pHP8080) containing the subcloned flbA gene. Furthermore, there was significantly reduced synthesis of the urease structural subunits in E. coli (pHP8080) containing the flbA gene, as determined by Western blot analysis with UreA and UreB antiserum. Thus, flagellar biosynthesis and urease activity may be linked in H. pylori. These results suggest that H. pylori genes may modulate urease activity.  (+info)

Identification of virulence genes of Helicobacter pylori by random insertion mutagenesis. (7/970)

The complete genome of the gram-negative bacterial pathogen Helicobacter pylori, an important etiological agent of gastroduodenal disease in humans, has recently been published. This sequence revealed that the putative products of roughly one-third of the open reading frames (ORFs) have no significant homology to any known proteins. To be able to analyze the functions of all ORFs, we constructed an integration plasmid for H. pylori and used it to generate a random mutant library in this organism. This integration plasmid, designated pBCalpha3, integrated randomly into the chromosome of H. pylori. To test the capacity of this library to identify virulence genes, subsets of this library were screened for urease-negative mutants and for nonmotile mutants. Three urease-negative mutants in a subset of 1,251 mutants (0.25%) and 5 nonmotile mutants in a subset of 180 mutants (2.7%) were identified. Analysis of the disrupted ORFs in the urease-negative mutants revealed that two had disruptions of genes of the urease locus, ureB and ureI, and the third had a disruption of a unrelated gene; a homologue of deaD, which encodes an RNA helicase. Analysis of the disrupted ORFs in the nonmotile mutants revealed one ORF encoding a homologue of the paralyzed flagellar protein, previously shown to be involved in motility in Campylobacter jejuni. The other four ORFs have not been implicated in motility before. Based on these data, we concluded that we have generated a random insertion library in H. pylori that allows for the functional identification of genes in H. pylori.  (+info)

Urease and hexadecylamine-urease films at the air-water interface: an x-ray reflection and grazing incidence x-ray diffraction study. (8/970)

We report the results of surface x-ray scattering measurements performed on urease and hexadecylamine-urease films at the air-aqueous solution interface. It is demonstrated that although hexadecylamine does not form a stable monolayer on the pure aqueous surface, it does self-assemble into a stable, well-organized structure when spread on top of a urease film at the air-water interface. It is also likely that protein and hexadecylamine domains coexist at the interface.  (+info)

Synthetic peptides mimicking antigenic epitope of Helicobacter pylori urease - Acta Biochimica Polonica - Volume 53, Issue 1 ...
Short peptides resembling the Helicobacter pylori urease antigen (UreB F8 Ser-Ile-Lys-Glu-Asp-Val-Gln-Phe) with deleted aspartic acid and glutamic acid residues, anchored through a triazine linker via the N-terminal moiety to cellulose plate were prepared. The peptides were used for binding of antibodies from sera of patients with medically confirmed atherosclerosis. Recognition of the peptides was also tested with anti-Jack beans urease antibodies. The important role of a Gly-Gly spacer separating the peptides from the cellulose support was shown. Different patterns of binding of antibodies from H. pylori infected patients and anti-Jack bean urease antibodies were observed only in the case of pentapeptides. The peptide Gly-Gly-Leu-Val-Phe-Lys-Thr was recognized by most of the tested sera ...
http://psjd.icm.edu.pl/psjd/element/bwmeta1.element.bwnjournal-article-abpv53p83kz
The role of Helicobacter pylori urease in the pathogenesis of gastritis and peptic ulceration. - ScienceOpenThe role of Helicobacter pylori urease in the pathogenesis of gastritis and peptic ulceration. - ScienceOpen
Helicobacter pylori produces a 550 kDa, multimeric, nickel-containing urease that catalyses the hydrolysis of urea to yield ammonia and carbonic acid. The ure gene cluster, comprised of seven genes, encodes the two structural subunits UreA (26.5 kDa) and UreB (60.3 kDa), and five accessory proteins: UreI, UreE, UreF, UreG and UreH. Accessory proteins are required for nickel ion insertion into the apoenzyme. The native protein consists of six copies each of UreA and UreB; two nickel ions are coordinated into each UreB active site. Urease is found in the cytosol, but may also localize on the surface (although this may be an artefact) and elicits a strong serum immunoglobulin response. Urease aids in colonization of the host by neutralizing gastric acid and providing ammonia for bacterial protein synthesis. Host defences are avoided by urease by continuing to neutralize acid locally and by shedding urease, which may be bound by immunoglobulin, from the surface of the bacterium. Host
https://www.scienceopen.com/document?vid=792a135f-5072-46cf-8b25-915a209696d2
Stool microbiome reveals diverse bacterial ureases as confounders of oral urea breath testing for Helicobacter pylori and...Stool microbiome reveals diverse bacterial ureases as confounders of oral urea breath testing for Helicobacter pylori and...
Detection of bacterial urease activity has been utilized successfully to diagnose Helicobacter pylori (H. pylori). While Mycobacterium tuberculosis (M. tuberculosis) also possesses an active urease, it is unknown whether detection of mycobacterial urease activity by oral urease breath test (UBT) can be exploited as a rapid point of care biomarker for tuberculosis (TB) in humans. We enrolled 34 individuals newly diagnosed with pulmonary TB and 46 healthy subjects in Bamako, Mali and performed oral UBT, mycobacterial sputum culture and H. pylori testing. Oral UBT had a sensitivity and specificity (95% CI) of 70% (46-88%) and 11% (3-26%), respectively, to diagnose culture-confirmed M. tuberculosis disease among patients without H. pylori, and 100% sensitivity (69-100%) and 11% specificity (3-26%) to diagnose H. pylori among patients without pulmonary TB. Stool microbiome analysis of controls without TB or H. pylori but with positive oral UBT detected high levels of non-H. pylori urease producing ...
https://www.physiciansweekly.com/stool-microbiome-reveals-diverse-bacterial-ureases-as-confounders-of-oral-urea-breath-testing-for-helicobacter-pylori-and-mycobacterium-tuberculosis-in-bamako-mali-2/
Use of polymerase chain reaction-amplified Helicobacter pylori urease structural genes for differentiation of isolates. |...Use of polymerase chain reaction-amplified Helicobacter pylori urease structural genes for differentiation of isolates. |...
Helicobacter pylori has been demonstrated as an etiologic agent of human gastritis and peptic ulcer formation. However, there is no straightforward basis to distinguish different isolates. We used the polymerase chain reaction (PCR) to amplify the urease structural subunit genes, ureA and ureB, which, when digested with appropriate restriction endonucleases, allow the differentiation of patterns on agarose gels. PCR amplification was possible with DNA rapidly extracted from H. pylori by alkaline lysis and phenol-chloroform. The 2.4-kb PCR products amplified from 22 clinical isolates and subjected to HaeII restriction endonuclease digestion produced 10 distinct patterns on agarose gels, with two patterns being shared between five and six strains. PCR amplification of the urease genes may enable the differentiation of closely related H. pylori strains by restriction digest analysis of PCR-amplified ureA and ureB genes. ...
https://jcm.asm.org/content/30/3/739?ijkey=3bc885e03ed4a2020b142b63e2583bac18d50ece&keytype2=tf_ipsecsha
Urease Test- Principle, Procedure and Result
Urea is a nitrogen-containing compound that is produced during decarboxylation of the amino acid arginine in the urea cycle. Urea is a major organic waste product of protein digestion in most vertebrate and is excreted in urine. Some bacteria have the ability to produce an enzyme urease as part of its metabolism to break down urea. The urease is a hydrolytic enzyme which attacks the carbon and nitrogen bond with the liberation of ammonia and carbon dioxide. It is a useful diagnostic test for identifying bacteria, especially to distinguish members of the genus Proteus from Gram-negative pathogens. Proteus vulgaris is an important and fast producer of urease.. Urease test is performed by growing test organism on urea agar slant or urea broth with phenol red as an indicator with pH6.8. During the incubation period, the organism capable of producing urease enzyme hydrolyse urea and produce ammonia that raises the pH level. As the pH increases, the phenol red changes from yellowish orange as the ...
https://www.biosciencetimes.com/study-notes/microbiology-study-notes/urease-test-principle-procedure-and-result/197/
Urease Test- Principle, Procedure and Result | Microbe NotesUrease Test- Principle, Procedure and Result | Microbe Notes
Urease Test- Principle, Procedure and Result. The objective is to determine the ability of the organism to hydrolyse urea by the action of urease enzyme. Urea is a nitrogen containing compound that is produced during decarboxylation of the amino acid arginine in the urea cycle. Proteus vulgaris Urease Positive.
https://microbenotes.com/urease-test-principle-procedure-and-result/
Characterization of Helicobacter pylori Nickel Metabolism Accessory Proteins Needed for Maturation of both Urease and...Characterization of Helicobacter pylori Nickel Metabolism Accessory Proteins Needed for Maturation of both Urease and...
Helicobacter pylori is a spiral, gram negative, microaerophilic bacterium that has been shown to be the etiological agent of gastritis and peptic ulcer disease (3, 4). It expresses two distinct nickel-containing enzymes, both of which are important for its virulence. These are a membrane bound [Ni-Fe] hydrogen-uptake hydrogenase, which permits respiratory-based energy production for the bacteria in the mucosa (19, 30), and an enzyme critical for early steps in colonization, urease (23, 25). Synthesis of metal-containing enzymes often requires the participation of accessory proteins, and the maturation of hydrogenase and urease are no exceptions. Indeed, the complete genome sequence of H. pylori reveals the presence of a full complement of urease (ureIEFGH) and hydrogenase (hypABCDEF) accessory genes (36). A number of studies exist that address these accessory genes in other bacteria and their role in the Ni-dependent maturation of urease and hydrogenase apoenzymes (7, 9, 13, 14, 15, 20, 22, 27, ...
https://jb.asm.org/content/185/3/726
Synthesis and activity of Helicobacter pylori urease and catalase at low pH. | GutSynthesis and activity of Helicobacter pylori urease and catalase at low pH. | Gut
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https://gut.bmj.com/content/40/1/25.alerts
Urease Assay Kit | BioAssay SystemsUrease Assay Kit | BioAssay Systems
BioAssay Systems Urease Assay Kit (DURE-100) measures urease activity and can be used to evaluate/screen for urease inhibitors. Urease reacts with urea to form ammonia, which is determined by the Berthelot method at 670nm. Samples: biological and environmental. Detection limit: 0.003 U/L.
https://www.bioassaysys.com/Urease-Assay-Kit.html
Periplasmic expression and one-step purification of urease subunit B of Helicobacter pylori | SpringerLinkPeriplasmic expression and one-step purification of urease subunit B of Helicobacter pylori | SpringerLink
UreB is one of the urease subunits of Helicobacter pylori and can be used as an excellent antigen candidate for H. pylori vaccination. Easy access to highl
https://link.springer.com/article/10.1007%2Fs11274-010-0540-2
Structure-based computational study of the catalytic and inhibition mechanisms of urease - Semantic ScholarStructure-based computational study of the catalytic and inhibition mechanisms of urease - Semantic Scholar
The viability of different mechanisms of catalysis and inhibition of the nickel-containing enzyme urease was explored using the available high-resolution structures of the enzyme isolated from Bacillus pasteurii in the native form and inhibited with several substrates. The structures and charge distribution of urea, its catalytic transition state, and three enzyme inhibitors were calculated using ab initio and density functional theory methods. The DOCK program suite was employed to determine families of structures of urease complexes characterized by docking energy scores indicative of their relative stability according to steric and electrostatic criteria. Adjustment of the parameters used by DOCK, in order to account for the presence of the metal ion in the active site, resulted in the calculation of best energy structures for the nickel-bound inhibitors β-mercaptoethanol, acetohydroxamic acid, and diamidophosphoric acid. These calculated structures are in good agreement with the experimentally
https://www.semanticscholar.org/paper/Structure-based-computational-study-of-the-catalyt-Musiani-Arnofi/e908085c78f59f5639fe016f758de5497e4ae9bf
A 4.6 KB DNA REGION OF RHIZOBIUM-MELILOTI INVOLVED IN DETERMINING UREASE AND HYDROGENASE ACTIVITIES CARRIES THE STRUCTURAL...
A 4.6 kb DNA region of the Rhizobium meliloti strain AK631 was found to contain seven open reading frames (ORFs), all oriented in the same direction. The putative gene products of four of these ORFs were highly homologous to UreA, UreB and UreC of Klebsiella aerogenes, Proteus mirabilis, Proteus vulgaris and Canavalia ensiformis. The overall organisation of the DNA region analysed was ORF1, ureA (ORF 2), ORF3, ureB (ORF4), ORF5, ORF6 and ureC (ORF7), indicating that the organisation of the urease structural genes in R. meliloti differs from that of other urease genes so far characterized. ORF1 was incomplete; only the 3 end of the coding region was present. The six complete ORFs coded for polypeptides of 11.1 (UreA), 8.9 (ORF3), 10.8 (UreB), 15.0 (ORF5), 13.8 (ORF6) and 60.7 kDa (UreC). No sequence homology to known polypeptides could be detected for the gene products of ORF1, ORF3, ORF5 and ORF6. Using a lacZ fusion and insertional mutagenesis it was shown that the seven ORFs identified were ...
https://pub.uni-bielefeld.de/publication/1629475
Structural basis for Ni2+ transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii.Structural basis for Ni2+ transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii.
article{139117, author = {Remaut, Han and SAFAROV, N and CIURLI, S and Van Beeumen, Jozef}, issn = {0021-9258}, journal = {JOURNAL OF BIOLOGICAL CHEMISTRY}, language = {eng}, number = {52}, pages = {49365--49370}, title = {Structural basis for Ni2+ transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii.}, volume = {276}, year = {2001 ...
https://biblio.ugent.be/publication/139117
Search Results
Ureaplasmas are unique among the mollicutes in possessing a very potent urease. Although protein and gene analysis of the ureaplasmal urease complex has shown that it resembles other procaryotic ureases in subunit structure and composition, the specific activity of the ureaplasmal urease is much higher and was estimated to exceed that of jack bean urease by about 100-fold. More importantly, ureaplasmas appear to be unique among procaryotes in requiring urea for growth. [...] The dependence of ureaplasmas on urea for growth has led to the hypothesis that intracellular urea hydrolysis and the resulting intracellular accumulation of ammonia/ammonium ions is coupled to ATP synthesis through a chemiosmotic type of mechanism. Experimental support for the generation of a transmembrane potential, with resultant ATP synthesis through the ureaplasmal F0F1-type ATPase, first provided by Romano et al. (1986, Medline: 3797330) was more recently extended and confirmed by Smith et al., 1993 [...] It is worth ...
http://stdgen.northwestern.edu/cgi-bin/gene_id_search.cgi?dbname=uure&gene_id=UU427
Search Results
Ureaplasmas are unique among the mollicutes in possessing a very potent urease. Although protein and gene analysis of the ureaplasmal urease complex has shown that it resembles other procaryotic ureases in subunit structure and composition, the specific activity of the ureaplasmal urease is much higher and was estimated to exceed that of jack bean urease by about 100-fold. More importantly, ureaplasmas appear to be unique among procaryotes in requiring urea for growth. [...] The dependence of ureaplasmas on urea for growth has led to the hypothesis that intracellular urea hydrolysis and the resulting intracellular accumulation of ammonia/ammonium ions is coupled to ATP synthesis through a chemiosmotic type of mechanism. Experimental support for the generation of a transmembrane potential, with resultant ATP synthesis through the ureaplasmal F0F1-type ATPase, first provided by Romano et al. (1986, Medline: 3797330) was more recently extended and confirmed by Smith et al., 1993 [...] It is worth ...
http://stdgen.northwestern.edu/cgi-bin/gene_id_search.cgi?dbname=uure&gene_id=UU431
ureC - Urease subunit alpha - Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) - ureC gene & proteinureC - Urease subunit alpha - Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) - ureC gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
https://www.uniprot.org/uniprot/Q8YQZ0
Rapid urease test - WikipediaRapid urease test - Wikipedia
The test is performed at the time of gastroscopy. A biopsy of mucosa is taken from the antrum of the stomach, and is placed into a medium containing urea and an indicator such as phenol red. The urease produced by H. pylori hydrolyzes urea to ammonia, which raises the pH of the medium, and changes the color of the specimen from yellow (NEGATIVE) to red (POSITIVE). Among different kinds of rapid urease tests (liquid-based, gel-based, dry cool) there is a design type with single-layer sensitive element - a layer impregnated simultaneously with urea and an indicator composition. Such a design bears the risk of false-positive result due to the pH value of the gastric biopsy when it is placed on the sensitive element. Excessive salivation and alkaline bile reflux into the stomach can shift the pH value of the biopsy of the stomach towards alkaline. Drugs that reduce the acidity of the stomach, also contribute to false positive results resulting from the alkalization. In each of these cases, the pH of ...
https://en.wikipedia.org/wiki/Rapid_urease_test
ureA - Urease subunit gamma - Staphylococcus aureus (strain MRSA252) - ureA gene & proteinureA - Urease subunit gamma - Staphylococcus aureus (strain MRSA252) - ureA gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
http://www.uniprot.org/uniprot/Q6GEE6
Urea R Broth
The principle of the urease test is to determine whether an organism has the ability to hydrolyze urea into two molecules of ammonia by the enzyme urease (urea amidohydrolase) which results in increased alkalinity of the medium. This increase in alkalinity is detected by the use of phenol red as the pH indicator, changing from a orange-red to a bright pink. (1) Urea R Broth is designed to be both rapid and sensitive for the detection of urease. Urea R Broth utilizes weak pH buffers monopotassium phosphate and disodium phosphate in very low concentrations in order to detect the smallest amount of alkali. Yeast extract acts as a source of nutrients, carbon, and nitrogen for those organisms incapable of using ammonia as a nitrogen source. (1) There are several different formulas for determining the urease reaction of a microorganism. For the strong urease producers like Proteus spp. there is Rustigian and Stuarts Urea Broth. For the weak urease producers such as Klebsiella pneumoniae and ...
https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/UreaRBroth.htm
The subunit structure of jack-bean urease | Biochemical JournalThe subunit structure of jack-bean urease | Biochemical Journal
1. Urease of specific activity 160-180 Sumner units/g. (Sumner, 1951) was purified from jack-bean meal. The preparation was pure on the basis of polyacryl-amide-gel electrophoresis and N-terminal studies. 2. By using both the 1-fluoro-2,4-dinitrobenzene method and the phenyl isothiocyanate method a single N-terminal methionine residue was found. 3. A single C-terminal sequence -Tyr-Leu-Phe was found by studies with carboxypeptidase A, carboxypeptidase B and hydrazinolysis. 4. N-Bromosuccinimide cleavage showed that five unique tryptophan sequences were present: Trp-Ala, Trp-Glu, Trp-Gly, Trp-Met and Trp-Arg. 5. Polyacrylamide-gel electrophoresis in sodium dodecyl sulphate showed that urease had a subunit molecular weight of 76000. 6. The yield of N- and C-terminal amino acids, the number of tryptic peptides and tryptophan sequences and the above polyacrylamide-gel electrophoretic measurement all suggest that urease contains a single structural subunit of molecular weight 75000.. ...
http://www.biochemj.org/content/113/4/669
Pharmaceutical Microbiology: Urease Test explained
The urease test is used to determine the ability of an organism to split urea, through the production of the enzyme urease. Two units of ammonia are formed with resulting alkalinity in the presence of the enzyme, and the increased pH is detected by a pH indicator ...
http://www.pharmamicroresources.com/2015/03/urease-test-explained.html
Rapid Urease Test for Diagnosis of H. Pylori Infection in Patients With Peptic Ulcer Bleeding - Full Text View - ClinicalTrials...Rapid Urease Test for Diagnosis of H. Pylori Infection in Patients With Peptic Ulcer Bleeding - Full Text View - ClinicalTrials...
Rapid urease test (RUT) is the most commonly used biopsy-based method to diagnose Helicobacter pylori (Hp) infection because of its simple, rapid and accurate characters. However, its sensitivity was reported to decrease during ulcer bleeding recently. So it is an important issue to avoid a false negative test in these patients. Siddique et al reported that the sensitivity of RUT could be increased when the biopsy number increased from 1 to 4. Other studies demonstrated that additional biopsy from gastric body would increased the sensitivity of RUT in patients with ulcer bleeding. Therefore, we design this study to see if increased number of biopsy or different location of biopsy could increase sensitivity of RUT in patients with gastroduodenal ulcer bleeding.. After receiving explanation and giving consent, these patients with gastric or duodenal ulcer bleeding diagnosed after endoscopic examination will be enrolled. Those who are unstable, have received antibiotic or continuous proton pump ...
https://clinicaltrials.gov/ct2/show/NCT01282853?recr=Open&cond=%22Helicobacter+Infections%22&rank=20
US5679570A - Device for performing urease tests on combined antrum/corpus biopsies to diagnose gastrointestinal illnesses ...
A device for carrying out urease tests for combined antrum/corpus biopsies to diagnose gastro-intestinal illnesses has a carrier plate, a schematic representation of the stomach on the plate, at least one opening in the plate at the locations corresponding to the corpus and the antrum in the schematic representation of the stomach, an evaluation scale for assessment of the urease test and an area for data on the patient and for clinical data.
https://patents.google.com/patent/US5679570A/en
Pre GI: Gene
General Information: This genus consists of organisms that colonize the mucosal layer of the gastrointestinal tract or are found enterohepatically (in the liver). It was only recently discovered (1983) by two Australians (Warren and Marshall) that this organism was associated with peptic ulcers. It is one of the most common chronic infectious organisms, and is found in half the worlds population. This organism attacks the gastric epithilial surface, resulting in chronic gastritis, and can cause more severe diseases including those that lead to gastric carcinomas and lymphomas, peptic ulcers, and severe diarrhea. It is an extracellular pathogen that persists in the gastric environment, which has a very low pH, by production of the urease enzyme, which converts urea to ammonia and carbon dioxide, a process which can counteract the acidic environment by production of a base. The toxins include cytolethal distending toxin, vacuolating cytotoxin (VacA) that induces host epithelial cell apopoptosis ...
http://pregi.bi.up.ac.za/pre_gi_gene.php?source_accession=NC_017358&gi_accession=NC_017358:182854
Pre GI: Gene
General Information: This genus consists of organisms that colonize the mucosal layer of the gastrointestinal tract or are found enterohepatically (in the liver). It was only recently discovered (1983) by two Australians (Warren and Marshall) that this organism was associated with peptic ulcers. It is one of the most common chronic infectious organisms, and is found in half the worlds population. This organism attacks the gastric epithilial surface, resulting in chronic gastritis, and can cause more severe diseases including those that lead to gastric carcinomas and lymphomas, peptic ulcers, and severe diarrhea. It is an extracellular pathogen that persists in the gastric environment, which has a very low pH, by production of the urease enzyme, which converts urea to ammonia and carbon dioxide, a process which can counteract the acidic environment by production of a base. The toxins include cytolethal distending toxin, vacuolating cytotoxin (VacA) that induces host epithelial cell apopoptosis ...
http://pregi.bi.up.ac.za/pre_gi_gene.php?source_accession=NC_018939&gi_accession=NC_018939:1583653
Citation tools | Infection and ImmunityCitation tools | Infection and Immunity
Dunn, B E et al Localization of Helicobacter pylori urease and heat shock protein in human gastric biopsies.. Infection and Immunity 65.4 (1997): 1181-1188. Web. 26 Feb. 2020. ...
https://iai.asm.org/highwire/citation/69118/download
NutriSphere products/Andersons | Page 2 | LawnSiteNutriSphere products/Andersons | Page 2 | LawnSite
Nitrisphere is not a coated or slow-release product. It is a chemical that inhibits the bacteria that carry urease enzyme, slowing the conversion of...
https://www.lawnsite.com/threads/nutrisphere-products-andersons.308591/page-2
Modulating the adjuvanticity of alum by co-administration of muramyl di-peptide (MDP) or Quil-A : WestminsterResearchModulating the adjuvanticity of alum by co-administration of muramyl di-peptide (MDP) or Quil-A : WestminsterResearch
The characterization of the immunological cascades of the innate immune system activated by pathogen associated molecular patterns (PAMP) recognized by pattern recognition receptors (PRR) have allowed the elucidation of the mechanisms underlying the immunomodulatory properties of adjuvants. Thus, the combinatorial use of adjuvants with specific, complementary functions is investigated to achieve tailored immune responses to subunit vaccines. We have previously shown how combinatorial administration of chitosan and cholera toxin B or muramyl-di-peptide (MDP) intranasally, but not intramuscularly, can allow small doses of MDP which, when administered alone cannot adjuvantise Helicobacter pylori urease (rUre), achieve an immunomodulatory effect through the specific physiological effect of chitosan. The aim of this study was to investigate if in the context of rUre the adjuvantising effect of MDP could be realized via the intramuscular route by combination with aluminium hydroxide, as compared with ...
https://westminsterresearch.westminster.ac.uk/item/92316/modulating-the-adjuvanticity-of-alum-by-co-administration-of-muramyl-di-peptide-mdp-or-quil-a
Bacterial Ureases And Its Role In Long-lasting Human DiseasesBacterial Ureases And Its Role In Long-lasting Human Diseases
Rheumatoid arthritis (RA), atherosclerosis (AR) and urinary tract infection (UTI) are long-lasting human diseases. RA and AR are chronic diseases with strong a...
https://www.omicsonline.org/proceedings/bacterial-ureases-and-its-role-in-longlasting-human-diseases-15129.html
Structure Cluster - 2UBP: STRUCTURE OF NATIVE UREASE FROM BACILLUS PASTEURII 3D Similarity Report PageStructure Cluster - 2UBP: STRUCTURE OF NATIVE UREASE FROM BACILLUS PASTEURII 3D Similarity Report Page
2UBP: A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels.
http://www.rcsb.org/pdb/explore/structureCluster.do?structureId=2UBP
Irtzas Micro Notes - Part 11
Most common cause of gastritis. Associated w/ duodenal ulcers and maybe cancer. , 20% of people , 30 years old. 40-60% of people 60 years old. G-, non spore-forming, curved to spiral, microaerophilic, catalase+, urease+, motile polar 5-6 flagella, coccoidal pordms under culture. Route of infection is unknown. Urease production allows survival at pH of 2.0. able to split ammonia from urea --, alkaline environment. Toxin & lipposac may damage mucosla cells. Treatment requires multiple antibiotics. Antacids heal ulcers, but have no effect on H. pylori. Amoxicillin and omerprazole 3X daily each has 91% cure rate. Resistance is spreading (metroidazole and clarithromycin). ...
http://irtza.com/micro-11.html
ASMscience | Yeasts
Yeasts represent one of the growth forms of fungi. They are unicellular, and reproduce mostly by budding or less often by fission. Most medically important yeasts originate from the division of Ascomycota or from the division of Basidiomycota. A simple biochemical reaction, the urease test, can usually distinguish the yeasts from each of the divisions. Ascomycetous yeasts are usually urease negative, whereas basidiomycetous yeasts are urease positive.
https://www.asmscience.org/content/book/10.1128/9781555819040.chap15
Acetohydroxamic acid Side Effects in Detail - Drugs.comAcetohydroxamic acid Side Effects in Detail - Drugs.com
Learn about the potential side effects of acetohydroxamic acid. Includes common and rare side effects information for consumers and healthcare professionals.
https://www.drugs.com/sfx/acetohydroxamic-acid-side-effects.html
Lithostat (Acetohydroxamic Acid Tablets): Side Effects, Interactions, Warning, Dosage & UsesLithostat (Acetohydroxamic Acid Tablets): Side Effects, Interactions, Warning, Dosage & Uses
Learn about Lithostat (Acetohydroxamic Acid Tablets) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and related medications.
https://www.rxlist.com/lithostat-drug.htm
GastroHep News StoryGastroHep News Story
Costs, expressed in Canadian dollars, were tabulated over a 1-year time period. The main outcome measured by the team was relief of symptoms. This was defined as the absence of symptom persistence or recurrence over the 12 months. The researchers compared performing a biopsy for the detection of H. pylori with a rapid urease test during gastroscopy, with that of not performing a biopsy. They also performed a secondary analysis, where they evaluated the cost-effectiveness of obtaining a biopsy specimen for histopathologic evaluation in patients after a negative rapid urease test.. The researchers found that endoscopy with biopsy and rapid urease testing costs $3940 per additional symptom-free patient, compared with endoscopy without biopsy. However, they determined that this result was sensitive to the difference in symptomatic recurrence rate at 1 year, in patients in whom H. pylori eradication was successful and unsuccessful. The team set this rate at 10%.. Only when the difference in ...
http://www.gastrohep.com/news/news.asp?id=2132
SAUSA300 2243 - AureoWikiSAUSA300 2243 - AureoWiki
Staphylococcus aureus; strain: USA300_FPR3757; locus tag: SAUSA300_2243 (SAUSA300_RS12380); symbol: ureG; product: urease accessory protein UreG
https://aureowiki.med.uni-greifswald.de/SAUSA300_2243
Study to Evaluate Safety and Immunogenicity of VPM1002 in Comparison With BCG in Newborn Infants in South Africa - Full Text...Study to Evaluate Safety and Immunogenicity of VPM1002 in Comparison With BCG in Newborn Infants in South Africa - Full Text...
Goal of VPM is the development of a recombinant urease C-deficient listeriolysin expressing BCG vaccine strain (VPM1002) as a safe, well tolerated and efficacious vaccine against tuberculosis (TB) for residents in endemic areas and persons at risk in non-endemic areas. The new vaccine should be at least as potent as the current strain and should be safer than BCG (Kaufmann, 2007a; Grode et al., 2005). The vaccine is formulated as live lyophilised bacteria to be re-suspended before intradermal injection. The preceding clinical trials in 80 volunteers in Germany and 24 volunteers in Bloemfontein, South Africa indicated immunogenicity and safety being sufficient for proceeding with the clinical development in newborn infants. Hence, the current study is commenced at Stellenbosch University, South Africa. This is the first investigation of VPM1002 in newborn infants ...
https://clinicaltrials.gov/show/NCT01479972?order=145
HP Urease recombinant proteinHP Urease recombinant protein
Buy HP Urease recombinant protein (MBS8506687) product datasheet at MyBioSource, Recombinant Proteins. Application: ELISA (EIA), CLIA, Radioimmunoassay (RIA), immunohistochemistry (IHC), Western Blot (WB)
https://www.mybiosource.com/prods/Recombinant-Protein/HP-Urease/datasheet.php?products_id=8506687
Urease Tablets Pack of 10 | Philip HarrisUrease Tablets Pack of 10 | Philip Harris
Urease tablets 10 tabsWorking Concentration - 1 - 2%pH range - 6 - 9Temperature range - 20 - 70°CSubstrate - 1 - 2% UreaProduct - Ammonia and CO2Source - JackbeansIts good practice to store enzymes in a refrigerator (5°C approx). This should give a shelf life of 12 months without significant loss of activity.All enzymes, as supplied, should be considered as 100% (unless otherwise specified) e.g. Cellulase supplied as 2g powder - 1g in 100mL gives a 1% working solution, or Pectinase supplied as 100mL - 1mL diluted to 100mL gives a 1% solution.pH - Recommended range for maximum activityTemperature - recommended range for maximum activityWorking Concentration - dilution of the Enzyme (as supplied) to this level should give an acceptable reation time.Substrate - substance to be reacted with the Enzyme, and appropriate concentrationProduct - substance produced by breakdown of the substrateSource - origin of the Enzyme
https://www.philipharris.co.uk/product/biology/human-body/enzymes/urease-tablets/b8a01983
Catalase, urease and gelatinase positive. Looking for a treatment. | Phoenix Rising ME / CFS ForumsCatalase, urease and gelatinase positive. Looking for a treatment. | Phoenix Rising ME / CFS Forums
Hi all, The results of the stool test last month showed that catalase, urease and gelatinase are positive. This explains my inflammation and leaky...
http://forums.phoenixrising.me/index.php?threads/catalase-urease-and-gelatinase-positive-looking-for-a-treatment.41635/
A1T9N4 | SWISS-MODEL Repository
SWISS-MODEL Repository entry for A1T9N4 (URE1_MYCVP), Urease subunit alpha. Mycolicibacterium vanbaalenii (strain DSM 7251 / JCM 13017 / NRRL B-24157 /PYR-1) (Mycobacterium vanbaalenii)
https://swissmodel.expasy.org/repository/uniprot/A1T9N4
RCSB PDB - Protein Feature View - Urease subunit beta - P41021 (URE2 SPOPA)RCSB PDB - Protein Feature View - Urease subunit beta - P41021 (URE2 SPOPA)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
http://www.rcsb.org/pdb/protein/P41021
Last reviewed 22 Mar 2016Last reviewed 22 Mar 2016
Lonsdale, W., Maurya, D., Wajrak, M., Tay, CY., Marshall, B., Alameh, K., (2017), Rapid measurement of urease activity using a potentiometric RuO2 pH sensor for detection of Helicobacter pylori. Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 242(1 April 2017), 1305-1308, Elsevier, DOI: 10.1016/j.snb.2016.06.024 ...
http://www.ecu.edu.au/schools/science/staff/profiles/lecturers/dr-magdalena-wajrak
Inhibition of urease by bismuth(III): Implications for the mechanism of action of bismuth drugsInhibition of urease by bismuth(III): Implications for the mechanism of action of bismuth drugs
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
https://www.growkudos.com/publications/10.1007%25252Fs10534-005-5449-0/reader
Urease | Application of Computational Methods for the Design of BACE-1 InhibitorsUrease | Application of Computational Methods for the Design of BACE-1 Inhibitors
Background and objectives: Coronary disease in chronic kidney disease (CKD) is explained partly simply by traditional cardiovascular risk elements; by uremia-specific elements; and by abnormalities of nutrient metabolism factors involved with its legislation and in MK 3207 HCl the vascular calcification procedure. subgroups described by tertiles of Gensini lesion intensity rating. Outcomes: The mean serum beliefs for FGF 23 in the complete study people was 28.1 ± 17.3 RU/ml as well as for fetuin A was 473.1 ± 156.2 μg/ml. Sufferers with eGFR 60 ml/min per 1 60 ml/min per 1.73 m2. The Gensini score values correlated with gender; arterial hypertension; and HDL cholesterol eGFR iPTH FGF 23 and fetuin A amounts. After the modifications for traditional and uremia-related cardiovascular risk factors the FGF 23 and fetuin A remained significant predictors of the Gensini score. Conclusions: This study suggests ...
http://world-of-links.com/category/urease/
UreaseUrease
A prospective open up label 3 month research was conducted to judge the feasibility and short-term clinical aftereffect of subcutaneous erythropoietin shots in sufferers with anemia and center failing preserved ejection fraction (HFPEF; EF=55±2). without significant adjustments in contractile condition. Keywords: Heart Failing Anemia Elderly Ejection Small percentage Introduction Anemia is CLG4B certainly a substantial […]. ...
http://expandingmarketing.com/category/urease/
TIGR03409TIGR03409
Members of this protein family are ABC transporter permease proteins associated with urea transport and metabolism. This protein is found in a conserved five-gene transport operon typically found adjacent to urease genes. It was shown in Cyanobacteria that disruption leads to the loss of high-affinity urea transport activity ...
http://www.jcvi.org/cgi-bin/tigrfams/HmmReportPage.cgi?acc=TIGR03409
Jaburetox: update on a urease-derived peptide | Journal of Venomous Animals and Toxins including Tropical Diseases | Full TextJaburetox: update on a urease-derived peptide | Journal of Venomous Animals and Toxins including Tropical Diseases | Full Text
Ureases (urea amidohydrolases; EC 3.5.1.5) are enzymes that catalyze the hydrolysis reaction of urea to ammonia and carbamate, which then decomposes through a spontaneous reaction of carbon dioxide in a second molecule of ammonia. These enzymes have been isolated from a wide variety of organisms including plants, fungi and bacteria [1, 2].. The urease extracted from Canavalia ensiformis seeds is one of the landmarks in the study of enzymes. It was the first enzyme to be crystallized, demonstrating that enzymes are proteins [3]. It was also the first one to be identified as a metalloenzyme containing nickel in its active site [4].. The classical urease, called jack bean urease (JBU), is composed of a polypeptide chain of 840 amino acid residues and has a molecular mass of 90 kDa. The minimum active form is a trimer of 270 kDa and it is often found in its native form as a hexamer of 540 kDa [5, 6]. The second isoform of jack bean urease, canatoxin (CNTX), was isolated from the seed and originally ...
https://jvat.biomedcentral.com/articles/10.1186/s40409-017-0122-y
Vitamin-C as anti-Helicobacter pylori agent: More prophylactic than curative- Critical review
bring about inactivation of biomolecules by oxidation.. Urease activity and AA Intriguingly, there is no consensus of opinion whether at all, and if yes, how AA interacts with ureases. In the early works the acid was reported to be a strong urease inhibitor (IC50=5.7 M) with the action prevented by cysteine32 . Later it was suggested that the inhibition was due to a reaction of DHA with the enzyme thiol groups33 . DHA, however, was later proved not to be an inhibitor, and instead it was proposed that the inhibition was provoked by the presence of traces of Cu2+ ions, which when reduced by AA, formed an inhibitory product Cu2 O34 . In refs. 32 C33, the buffers applied were not specified. A subsequent study performed in 0.7M phosphate buffer pH 6.835 , showed that neither ascorbic (up to 1.1mM), nor dehydroascorbic or 2,3-diketogulonic acid themselves were urease inhibitors, but that ascorbic and 2,3-diketogulonic acid inhibited the enzyme in the presence of Cu2+ (15.6 M). The inhibition by AA was ...
http://nutriforce.cn/Vitamin-C%20as%20anti-Helicobacter%20pylori%20agent%20More%20prophylactic%20than%20curative-%20Critical%20review.htm
Ammonia Toxicity - Dr. Susan L. Marra, PLLCAmmonia Toxicity - Dr. Susan L. Marra, PLLC
Most patients with neurological Lyme Disease and coinfections (i.e., MS, ALS, Parkinsons Disease, Autism), have elevated levels of ammonia in the brain which is neurotoxic. This is due to the fact that the spirochete, Borrelia burgdorferi, produces urease enzymes, and ammonia accumulation results. Sometimes ammonia levels will be elevated in the blood, but many times they are also normal. This is because the ammonia concentration is localized in specific tissues (brain), where the concentration is not high enough to be detected in the blood. But that doesnt mean that ammonia is not causing destruction. It is VERY neurotoxic, and needs to be removed with herbal supplements and diet changes ...
http://drsusanmarra.com/ammonia-toxicity/
Publications - Institute for Bioengineering of Catalonia (IBEC)Publications - Institute for Bioengineering of Catalonia (IBEC)
Bio-catalytic micro- and nanomotors self-propel by the enzymatic conversion of substrates into products. Despite the advances in the field, the fundamental aspects underlying enzyme-powered self-propulsion have rarely been studied. In this work, we select four enzymes (urease, acetylcholinesterase, glucose oxidase, and aldolase) to be attached on silica microcapsules and study how their turnover number and conformational dynamics affect the self-propulsion, combining both an experimental and molecular dynamics simulations approach. Urease and acetylcholinesterase, the enzymes with higher catalytic rates, are the only enzymes capable of producing active motion. Molecular dynamics simulations reveal that urease and acetylcholinesterase display the highest degree of flexibility near the active site, which could play a role on the catalytic process. We experimentally assess this hypothesis for urease micromotors through competitive inhibition (acetohydroxamic acid) and increasing enzyme rigidity ...
http://www.ibecbarcelona.eu/for-researchers/publications/Biocatalysis/
Will the roads of the future be made of sand by the bacteria Bacillus Pasteurii? - iBox createWill the roads of the future be made of sand by the bacteria Bacillus Pasteurii? - iBox create
Despite its widespread use for roads, asphalt presents significant problems related to the environment (due to the use of bitumen compounds), sustainability (due to the use of oil) and its variable price, which makes it necessary to consider at least new materials for road construction. It is possible that the future of more sustainable and economical road construction lies in a bacterium called Bacillus Pasteurii, which combined with sand produces solid sandstone very quickly, forming a ready-to-use pavement ...
https://iboxcreate.es/en/seran-de-arena-las-carreteras-del-futuro-gracias-a-la-bacteria-bacillus-pasteurii/
Bacillus pasteurii info needed - MicrobiologyBacillus pasteurii info needed - Microbiology
The problem is Id like to know if anybody have described Bacillus pasteurii as a antibiotic productor before, and in this case, what substance could be. I find no data base about this kind of information in the Web (maybe due the industrial secret?) only taxonomic and poorly descriptive bases. I will thank you very much if could link me to any interesting page in this sense ...
http://www.protocol-online.org/biology-forums/posts/5339.html
Domain combinations for Urease, gamma-subunit superfamily in Bacillus pseudofirmus OF4Domain combinations for Urease, gamma-subunit superfamily in Bacillus pseudofirmus OF4
Domain combinations containing the Urease, gamma-subunit superfamily in Bacillus pseudofirmus OF4. Domain architectures illustrate each occurrence of the Urease, gamma-subunit superfamily.
http://supfam.mrc-lmb.cam.ac.uk/SUPERFAMILY/cgi-bin/allcombs.cgi?genome=aN
Hard Shell, Soft CoreHard Shell, Soft Core
Urease is found in plant seeds, bacteria, and various sea creatures. It splits urea into carbon dioxide and ammonia. Ammonia is an alkaline substance; when urease is active, the pH value on the surface of the enzyme rises. Because of the negative charge on its surface, urease has a high affinity for positively charged metal ions. In a zinc nitrate solution, the zinc ions aggregate around the urease. The local pH value on the surface of the enzyme can be finely tuned to values ideal for the formation of zinc oxide. The increasing zinc ion concentration at a suitable pH value catalyzes the formation and growth of ZnO crystals. The enzyme cores thus become surrounded by nanoshells of zinc oxide. These have a diameter of about 18 nm. By using slightly modified urease molecules, the size of the zinc oxide shells can be varied. Author: Hiroshi Matsui, Hunter College, City University of New York (USA), http://www.hunter.cuny.edu/chem/faculty_files/matsui/matsui.html. ...
http://www.innovations-report.com/html/reports/life-sciences/report-113082.html
CLO test | definition of CLO test by Medical dictionaryCLO test | definition of CLO test by Medical dictionary
Looking for online definition of CLO test in the Medical Dictionary? CLO test explanation free. What is CLO test? Meaning of CLO test medical term. What does CLO test mean?
http://medical-dictionary.thefreedictionary.com/CLO+test
Trust Drugstore: Reliable viagra uk with meds care!Trust Drugstore: Reliable viagra uk with meds care!
Anti- the antidotes to warfarin therapy should be able to: Haemostasis embolism outline the rationale for this condition. N certain groups of drugs on lipid levels cholesterol diet. Homework have the applied anatomy and physiology, 5nd edn, by elaine n marieb. Of gastric pain, vomiting, nausea and the cancer features assessment a notable rapidly resolves, although in our block imposed by the team of physicians and nurses should be followed carefully. Palpation may be detected in urine cul- ture before the symptoms of brain disturbances disorders such as jelly sh, molluscs and octopus, in the form of a urease-producing bacterial infection of human anatomy, ed 3, plate 531.) chapter 2 lower limb between the antihyperlipidaemic drug and some antifungal drugs). ambiguous clinical evaluation 45 table 2.5 joints of spine, fingers, knee, and hip flexion are eliminated, extensors thermoregulation. 2. Alonso rc, nacenta sb, martinez pd, guerrero as, fuentes cg. 2001;27(5):470 4. Chapter 43 tips to build ...
https://kirstieennisfoundation.com/dysfunction/reliable-viagra-uk/35/
Rapid Test for Urease and Phenylalanine Deaminase Production -...Rapid Test for Urease and Phenylalanine Deaminase Production -...
Rapid Test for Urease and Phenylalanine Deaminase Production: A rapid urea-phenylalanine medium was effective for the identification of Proteus and, with one ex
https://www.mysciencework.com/publication/show/rapid-test-for-urease-and-phenylalanine-deaminase-production
Scientists discover a natural | EurekAlert! Science News ReleasesScientists discover a natural | EurekAlert! Science News Releases
Chemists created a nonpermanent adhesive from a natural chemical reaction that can be used in the biomedical field. This discovery may benefit tissue repair or drug delivery. The scientific journal Angewandte Chemie recently published this collaborative work between LSU and University of Sheffield researchers.. The scientists studied the natural chemical process that occurs when urea, a molecule found in urine, is broken down by the enzyme urease, which produces ammonia and carbon dioxide. Based on previous studies, scientists know how long it takes urease to break down urea, which can be used to create a chemical process called a pH clock reaction. They chose urea and urease because it is one of the few nontoxic and natural clock reactions. By adding water and two chemicals -- a sulfur-based thiol and a synthetic acrylate--during the urea-urease clock reaction, the researchers were able to create a thin, water-soluble adhesive gel.. LSU doctoral candidate Elizabeth Jee led the study. Jee ...
https://www.eurekalert.org/news-releases/748110
Health Questions and Answer - Answer to Specific Health QuestionHealth Questions and Answer - Answer to Specific Health Question
The organism lives within or beneath the gastric mucous layer, somewhat protected from stomach acid. H. pylori has potent urease activity, which hydrolyzes urea to ammonia and bicarbonate and increases its resistance to the stomachs low pH environment. H. pylori recognizes and binds to specific receptors expressed by gastric epithelial cells and, therefore, is able to adhere tightly to the epithelial cell surface. This attachment process may morphologically or functionally alter the epithelial cell. The organism has been found adherent to ectopic gastric epithelium throughout the GI tract, that is, esophagus (Barretts), duodenum (gastric metaplasia), small intestine (Meckels diverticulum), and rectum (ectopic patches of gastric mucosa).. Reference:. ...
http://www.randyamy.com/question-where-in-the-gastrointestinal-gi-tract-does-helicobacter-pylori-live
Use of XAS for the elucidation of metal structure and function: Applications to nickel biochemistry, molecular toxicology, and...
TY - JOUR. T1 - Use of XAS for the elucidation of metal structure and function. T2 - Applications to nickel biochemistry, molecular toxicology, and carcinogenesis. AU - Carrington, Paul E.. AU - Al-Mjeni, Faizah. AU - Zoroddu, Maria A.. AU - Costa, Max. AU - Maroney, Michael J.. PY - 2002/10. Y1 - 2002/10. N2 - Nickel has been shown to be an essential trace element involved in the metabolism of several species of bacteria, archea, and plants. In these organisms, nickel is involved in enzymes that catalyze both non-redox (e.g., urease, glyoxalase I) and redox (e.g., hydrogenase, carbon monoxide dehydrogenase, superoxide dismutase) reactions, and proteins involved in the transprt, strage, metallocenter assembly, and regulation of nickel concentration have evolved. Studies of structure/function relationship in nickel biochemistry reveal that cysteine ligands are used to stabilize the Ni(III/II) redox couple. Certain nickel compounds have also been shown to be potent human carcinogens. A likely ...
https://squ.pure.elsevier.com/en/publications/use-of-xas-for-the-elucidation-of-metal-structure-and-function-ap
BACILLUS PASTEURII MEDIUM | PTCCBACILLUS PASTEURII MEDIUM | PTCC
Medium 2 plus 2% urea.. 10 ml filter-sterilized 20% urea solution is added asptically post autoclaving to 100ml cooled molten nutrent agar. The medium is then immediately dispensed aseptically. For sporulation enhancement add 10 mg/l MnSO4 x H2O.. ...
http://irost.org/ptcc/ptccdb/node/35
Science: Enzyme takes toxins out of the soil | New ScientistScience: Enzyme takes toxins out of the soil | New Scientist
Urease, an enzyme which degrades the naturally occurring compound urea into ammonia and carbon dioxide, can do the same to the synthetic fertiliser cyanamide, say biochemists in Germany. They believe some forms of urease may act on a wide range of compounds, and so be able to render harmless some of the unnatural chemicals in …
https://www.newscientist.com/article/mg13518323-200-science-enzyme-takes-toxins-out-of-the-soil/
James Madison University - Orientation at UREC: UREC Fest!James Madison University - Orientation at UREC: UREC Fest!
Welcome to UREC First-Year and Transfer! We are incredibly excited to introduce you to all UREC has to offer and we have a special event planned to help you get acquainted to our facilities, programs and services. Remember to bring your JACard to swipe in and out of UREC!. UREC-Fest (1787 Orientation) Thursday, August 25, 2016, 9-11pm. Each year, UREC hosts a welcome event for new students during JMU 1787 Orientation. Be among the first to explore the newly renovated UREC! Join in the fun by swimming and playing in the pools, climbing in the new Adventure Center, playing a pick-up sport in the Sports Forums, or trying a group exercise class. Food, music, and giveaways will be available! If you plan to work out or swim, please wear appropriate attire. Your JACard is your admission ticket. Learn more about this event!. UREC Homepage / Facebook / Twitter. #defaultregion { padding-top: 24px; } @media all and (min-width:120px){ #defaultregion { padding-top: 0px; } } @media all and (min-width:320px){ ...
http://www.jmu.edu/news/recreation/2016/08/24-orientation.shtml
Planning exercise - A-Level Science - Marked by Teachers.comPlanning exercise - A-Level Science - Marked by Teachers.com
Introduction. Planning exercise Aim: To plan an investigation to find out how changes in the consumption of protein in the diet influence the excretion of urea in humans. Introduction: I will need to measure the amount of urea produced by people with different protein intakes. I will use the enzyme urease to break the urea down into the alkaline ammonium carbonate and titrate this against an acid to find its concentration. Prediction: I think that the more protein the consumer has in their diet, the more urea will be secreted, therefore the ammonium carbonate will have a higher concentration. Hypothesis: Chains of amino acids make proteins. When too much protein is consumed those not needed for protein synthesis go through a process called deamination in the liver. The amino group and a hydrogen are removed from the amino acid and form ammonia (NH ) . This is then converted to urea which is less toxic and soluble. NO + CO = (NH ) CO. The urea is carried away by the blood to the kidney where it ...
http://www.markedbyteachers.com/as-and-a-level/science/planning-exercise.html
Animal HelicobactersAnimal Helicobacters
The picture on the right is a dog helicobacter (H.Helmanii) from a patient of Dr. Marshalls who kept two red setters in his house all his life.. The animal helicobacters can be detected on biopsy, or with urease tests or breath tests. Next time your cat has fur balls , think again. It could be Helicobacters! You might try giving the cat some Pepto-Bismol, 2 mls in a syringe three times per day for two days.. ...
https://helico.com/animals.html
The role of gastric urease in acid secretion | Biochemical JournalThe role of gastric urease in acid secretion | Biochemical Journal
Thank you for your interest in spreading the word about Biochemical Journal.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
http://www.biochemj.org/content/50/1/119
Urease, gamma subunit superfamily (IPR036463) | InterPro | EMBL-EBIUrease, gamma subunit superfamily (IPR036463) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
http://www.ebi.ac.uk/interpro/entry/IPR036463
Cloning, sequencing and molecular characterisation of a crypti...Cloning, sequencing and molecular characterisation of a crypti...
Cloning, sequencing and molecular characterisation of a cryptic plasmid from a urease-positive thermophilic Campylobacter (UPTC) isolate.: Cloning, sequencing a
https://www.mysciencework.com/publication/show/cloning-sequencing-molecular-characterisation-cryptic-plasmid-from-urease-positive-thermophilic-campylobacter-uptc-isol-da6abfc2
Drugstore Plus: Non rx canada lowest price!Drugstore Plus: Non rx canada lowest price!
Non rx canada - But the body and downward to the guy who was put forward a somewhat similar canada non rx theory in which there is no hypotension. Avalos la, roberts sc, kaskutas la, et al. Remove specimen vaginally and avoid uncontained morcellation. Hippocampi pl. Recognise as epileptic: (a) a urease which hydrolyses urea into ammonia.
https://abt.edu/bestsellers/non-rx-canada/22/
Volume 1, Issue 4
An Electrochemical Aptasensor Using Coaxial Capillary with Magnetic Nanoparticle, Urease Catalysis and PCB Electrode for Rapid and Sensitive Detection of Escherichia coli O157:H7 ...
http://ntno.org/v1i4
Urejaza - WikipediaUrejaza - Wikipedia
Varner, J.E. (1960). Urease. u: Boyer, P.D., Lardy, H. and Myrbäck, K.. The Enzymes. 4 (2nd izd.). New York: Academic Press. str. 247-256. ...
https://sh.wikipedia.org/wiki/Urejaza
Fluke 721Ex Precision Pressure Calibrator | FlukeFluke 721Ex Precision Pressure Calibrator | Fluke
psi, bar, mbar, kPa, kgcm2, cmH 2O @ 4°C, cmH 2O @ 20°C, mH 2O @ 4°C, mH 2O @ 20°C, inH 2O @ 4°C, inH 2O @ 20°C, inH 2O @ 60°F, mmHg @ 0°C, inHg @ 0°C, ftH 2O @ 4°C, ftH 2O @ 20°C, ftH 2O @ 60° ...
https://www.fluke.com/id-id/produk/alat-kalibrasi/kalibrator-tekanan/fluke-721ex
Appliance Repairs in West Yorkshire | Yorkshire AppliancesAppliance Repairs in West Yorkshire | Yorkshire Appliances
We cover all appliance repairs and servicing for clients throughout West Yorkshire. Contact us today, in Shipley, to discover more about our services.
https://www.yorkshireappliances.com/repairs-and-servicing-appliance-repairs-west-yorkshire
METAP2METAP2
... urease; arginase; several phosphatases and phosphoesterases-that includes two bridging carboxylate ligands and a bridging water ...
https://en.wikipedia.org/wiki/METAP2
Proteus penneriProteus penneri
A highly active urease produced by P. penneri may also have a role in the establishment of an infectious process. The ... Moreover, the urease enzyme of P. penneri is thought to be a significant cause of kidney stone formation. Consistent with this ... Griffith DP, Musher DM, Itin C (1976). "Urease. The primary cause of infection-induced urinary stones". Invest Urol. 13 (5): ...
https://en.wikipedia.org/wiki/Proteus_penneri
Helicobacter pyloriHelicobacter pylori
Urease knockout mutants are incapable of colonization. In fact, urease expression is not only required for establishing initial ... As mentioned above, H. pylori produce large amounts of urease to produce ammonia as one of its adaptation methods to overcome ... It produces oxidase, catalase, and urease. H. pylori possesses five major outer membrane protein families. The largest family ... Mobley HL (1 January 2001). "Urease". In Mobley HL, Mendz GL, Hazell SL (eds.). Helicobacter pylori: Physiology and Genetics. ...
https://en.wikipedia.org/wiki/Helicobacter_pylori
Timeline of peptic ulcer disease and Helicobacter pyloriTimeline of peptic ulcer disease and Helicobacter pylori
1953 Dintzis and Hastings are able to stop urease production in mice with antibiotics, suggesting a relationship between urease ... Lieber, Charles S. J., "How it was discovered in Belgium and the USA (1955-1976) that Gastric Urease was Caused by a Bacterial ... call into question the extent of urease produced by bacteria in mice, as an argument against the bacterial theory of PUD. 1960 ... 1924 Luck and Seth discover urease in the human stomach, which they believe is naturally occurring. 1925 Hoffman injects a ...
https://en.wikipedia.org/wiki/Timeline_of_peptic_ulcer_disease_and_Helicobacter_pylori
TaylorellaTaylorella
Oxidase = positive , Catalase = positive , Indole = negative , H2S test = negative , Urease = negative , Motility =non-motile ...
https://en.wikipedia.org/wiki/Taylorella
Salicylhydroxamic acidSalicylhydroxamic acid
... (SHA or SHAM) is a drug that is a potent and irreversible enzyme inhibitor of the urease enzyme in ... The molecule is similar to urea but is not hydrolyzable by urease; it thus disrupts the bacteria's metabolism through ... Acetohydroxamic acid Fishbein, W; Carbone, P (1965). "Urease catalysis. ii. Inhibition of the enzyme by hydroxyurea, ...
https://en.wikipedia.org/wiki/Salicylhydroxamic_acid
Acetohydroxamic acidAcetohydroxamic acid
The molecule is similar to urea but is not hydrolyzable by urease; it thus disrupts the bacteria's metabolism through ... Salicylhydroxamic acid Fishbein WN, Carbone PP (June 1965). "Urease Catalysis. Ii. Inhibition of the Enzyme by Hydroxyurea, ... is a drug that is a potent and irreversible enzyme inhibitor of the urease enzyme in various bacteria and plants; it is usually ...
https://en.wikipedia.org/wiki/Acetohydroxamic_acid
Lysinibacillus fusiformisLysinibacillus fusiformis
As of now, the only known factor that distinguishes these two species is that L. fusiformis is positive for urease. This means ... "Urease Test Protocol." ASM MicrobeLibrary. American Society For Microbiology, 11 Nov. 2011. Web. 29 Apr. 2014. Nakamura, L. K ...
https://en.wikipedia.org/wiki/Lysinibacillus_fusiformis
Naganishia albidaNaganishia albida
... produces urease. While this species is most frequently found in water and plants and is also found on animal ...
https://en.wikipedia.org/wiki/Naganishia_albida
Yersinia pseudotuberculosisYersinia pseudotuberculosis
The bacterium is urease positive. In animals, Y. pseudotuberculosis can cause tuberculosis-like symptoms, including localized ...
https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis
Mycoplasma oraleMycoplasma orale
Bacteria in the genus Ureaplasma are known commensals in humans and possess the enzyme Urease (catalyzes urea to carbon dioxide ... Blanchard, A., Razin, S., Kenny, G. E., & Barile, M. F. (1988). "Characteristics of Ureaplasma urealyticum urease". Journal of ...
https://en.wikipedia.org/wiki/Mycoplasma_orale
Edwin C. WebbEdwin C. Webb
Blakeley, Robert L.; Webb, Edwin C.; Zerner, Burt (1969). "Jack bean urease (EC 3.5.1.5). A new purification and reliable rate ... After moving to Queensland Webb collaborated with Burt Zerner on Jack bean urease, starting with a study of its purification ...
https://en.wikipedia.org/wiki/Edwin_C._Webb
Achromobacter xylosoxidansAchromobacter xylosoxidans
It is urease and indole-negative. It produces acid oxidatively from xylose, but not from lactose, maltose, mannitol, or sucrose ...
https://en.wikipedia.org/wiki/Achromobacter_xylosoxidans
Microsporum gallinaeMicrosporum gallinae
Additionally, M. gallinae is urease positive. During sporulation, septate hyphae, with macroconidia with tappered tips, and ...
https://en.wikipedia.org/wiki/Microsporum_gallinae
Naganishia bhutanensisNaganishia bhutanensis
This species does not produce urease. Baharaeen S. and Vishniac H. S. (1981) Budding Morphology of a Psychrophilic Cryptococcus ...
https://en.wikipedia.org/wiki/Naganishia_bhutanensis
Research Centre for Emerging and Reemerging Infectious DiseasesResearch Centre for Emerging and Reemerging Infectious Diseases
On the Urease of Yersin's Bacillus. 1964. Baltazard, M., et al., C. Interepizootic conservation of the plague in inveterate ...
https://en.wikipedia.org/wiki/Research_Centre_for_Emerging_and_Reemerging_Infectious_Diseases
Colorimetric analysisColorimetric analysis
... enzyme urease)--> ammonium carbonate Ammonium carbonate + phenol + hypochlorite ----> colored complex In this case, only the ...
https://en.wikipedia.org/wiki/Colorimetric_analysis
Vincent SchaeferVincent Schaefer
"Activities of Urease and Pepsin Monolayers". J. Am. Chem. Soc. 60 (6): 1351-1360. doi:10.1021/ja01273a023. ISSN 0002-7863. ...
https://en.wikipedia.org/wiki/Vincent_Schaefer
Micrococcus luteusMicrococcus luteus
It is urease and catalase positive. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the ...
https://en.wikipedia.org/wiki/Micrococcus_luteus
Sporosarcina ureaeSporosarcina ureae
S. ureae is one of the bacteria that can make use of urea with the enzyme urease. It is often found in soil, and forms the ... McCoy, D.D.; Cetin, A.; Hausinger, R.P. (1992). "Characterization of urease from Sporosarcina ureae". Archives of Microbiology ...
https://en.wikipedia.org/wiki/Sporosarcina_ureae
Mycobacterium bohemicumMycobacterium bohemicum
Enzymatic activity- weak positive test for urease. To identify M. bohemicum, its resulting sequence was isolated and compared ...
https://en.wikipedia.org/wiki/Mycobacterium_bohemicum
Elizabethkingia meningosepticaElizabethkingia meningoseptica
It is negative by the urease test. In general, it is negative by the nitrate reductase test, although some strains are positive ...
https://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica
Diagnostic microbiologyDiagnostic microbiology
The urease agar slant is used to measure an organism's ability to produce urease, an enzyme capable to digesting urea in carbon ... "Urease Test- Principle, Media, Procedure and Result". Online Microbiology Notes. 2015-09-20. Retrieved 2017-04-03. MacFaddin, J ... indicating the presence of urease production. The Voges-Proskauer test detects whether a bacterium is producing the product ...
https://en.wikipedia.org/wiki/Diagnostic_microbiology
Proteus mirabilisProteus mirabilis
This rod-shaped bacterium has the ability to produce high levels of urease, which hydrolyzes urea to ammonia (NH3), so makes ... It shows swarming motility and urease activity. P. mirabilis causes 90% of all Proteus infections in humans. It is widely ...
https://en.wikipedia.org/wiki/Proteus_mirabilis
Bordetella trematumBordetella trematum
It is indole negative and urease negative. DNA-rRNA hybridization was used to place B. trematum LMG 13506T in the family ...
https://en.wikipedia.org/wiki/Bordetella_trematum
MycoplasmataceaeMycoplasmataceae
As the name implies, ureaplasma is urease positive. This genera is distinct from other genera in Mollicutes in that it ...
https://en.wikipedia.org/wiki/Mycoplasmataceae
Candida tropicalisCandida tropicalis
This test is to detect yeast's ability to produce enzyme urease. Once there's a compatible substrate, urease will split urea to ... C. tropicalis is found to be urease negative. This test uses the azo dye, diazonium blue B which differentially stains cells ...
https://en.wikipedia.org/wiki/Candida_tropicalis
Burkholderia gladioliBurkholderia gladioli
They are aerobic, catalase positive, urease positive, nonsporeformers. They grow on MacConkey agar, but do not ferment the ...
https://en.wikipedia.org/wiki/Burkholderia_gladioli
UreaplasmaUreaplasma
As the name imples, Ureaplasma is urease positive. The currently accepted taxonomy is based on the List of Prokaryotic names ...
https://en.wikipedia.org/wiki/Ureaplasma
BrucellaBrucella
Testing for urease would successfully accomplish the task; it is positive for Brucella and negative for Salmonella. Brucella ...
https://en.wikipedia.org/wiki/Brucella
EN (en)EN (en)
Although many bacteria are ureolytic, and in some cases urease acts as a virulence factor, the urease phenotype has not been ... Urease production was inhibited by urea and induced under growth conditions where the availability of nitrogen sources was ... This represents the first report of a plasmid-encoded urease in a gram-positive bacterium. The C. perfringens enzyme was highly ... similar to the ureases of other bacteria and cross-reacted with antibodies raised against the urease purified from Helicobacter ...
https://research.pasteur.fr/en/publication/clostridium-perfringens-urease-genes-are-plasmid-borne/
UREASE ACTIVITY AND NITROGEN MINERALIZATION KINETICS AS AFFECTED BY TEMPERATURE AND UREA INPUT RATE IN SOUTHERN CHILEAN ANDISOLSUREASE ACTIVITY AND NITROGEN MINERALIZATION KINETICS AS AFFECTED BY TEMPERATURE AND UREA INPUT RATE IN SOUTHERN CHILEAN ANDISOLS
In FS, urease activity was the highest at 24 h, and it decreased at 48 h as a consequence of substrate depletion. On the other ... Our results showed that urease activity was higher in Piedras Negras soil (PNS) than Freiré soil (FS) at 24 and 48 h, and the ... CARTES, Paula; JARA, Alejandra A; DEMANET, Rolando and MORA, María de la Luz. UREASE ACTIVITY AND NITROGEN MINERALIZATION ... Incubation studies were carried out to evaluate the effect of temperature and urea supply level on both the urease activity and ...
https://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0718-27912009000100006&lng=en&nrm=iso&tlng=en
Brucella melitensis Differs from B. suis in Growth and Urease Activity In-Vitro, and Infectivity in Fisher-344 Rats In-VivoBrucella melitensis Differs from B. suis in Growth and Urease Activity In-Vitro, and Infectivity in Fisher-344 Rats In-Vivo
In cell-free extracts, the B. suis urease showed 12 times greater specific activity than the B. melitensis urease. When Fisher- ... The wild type and the urease deficient strains of B. suis did not differ from each other in terms of recovery from spleen or ... and urease activity has no influence on Brucella infection using an IP route. ... Importance of urease activity on pathogenic differences among Brucella species was evaluated. ...
https://www.scirp.org/journal/paperinformation.aspx?paperid=28990
The competitive inhibition of of the urease-catalyzed hydrolysis of urea by phosphate - CaltechAUTHORSThe competitive inhibition of of the urease-catalyzed hydrolysis of urea by phosphate - CaltechAUTHORS
The urease-catalyzed hydrolysis of urea has been found to be competitively inhibited by phosphate at pH 7.0 and 25°. The ... Harmon, Kent M. and Niemann, Carl (1949) The competitive inhibition of of the urease-catalyzed hydrolysis of urea by phosphate. ... The competitive inhibition of of the urease-catalyzed hydrolysis of urea by phosphate ... urea-urease system has been found to be approximately 0.003 M urea and the comparable constant defining the phosphate-urease ...
https://authors.library.caltech.edu/5840/
Antioxidant and urease inhibition activity of methanol extract of Hibiscus schizopetalus (Mast) Hook.Antioxidant and urease inhibition activity of methanol extract of Hibiscus schizopetalus (Mast) Hook.
... urease inhibition at the same concentrations. The percentage urease inhibition of standard Thiourea was found to be 98.2%, IC50 ... Antioxidant and urease inhibition activity of methanol extract of Hibiscus schizopetalus (Mast) Hook.. J Pharmacogn Phytochem ... Research on urease inhibitions from natural source yielded several imperative therapeutic drugs that are useful in gastric and ... HFE extract showed maximum urease activity with percentage inhibition of 55.5%, IC50 80.1 ± 0.87 mg/ml. While least inhibitory ...
https://www.phytojournal.com/archives/2014.v2.i6.268/antioxidant-and-urease-inhibition-activity-of-methanol-extract-of-hibiscus-schizopetalus-mast-hook
Transformacoes quimicas e microbiologicas e avaliacao da microflora urease-positiva em cacao armazenado sob refrigeracaoTransformacoes quimicas e microbiologicas e avaliacao da microflora urease-positiva em cacao armazenado sob refrigeracao
Polish Journal of Environmental Studies - Keyword activity of urease Polish Journal of Environmental Studies - Keyword activity of urease
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more ...
http://www.pjoes.com/Keyword-activity+of+urease/162539
Optimize crop fertility with soil sampling, urease inhibitors - Rice FarmingOptimize crop fertility with soil sampling, urease inhibitors - Rice Farming
I think most rice farmers in Texas use a urease inhibitor, and I think this a good practice because of Southeast Texas erratic ... When urea is applied to soil, microbes in the soil release an enzyme called urease, which catalyzes the breakdown of urea to ... If you have trouble getting water across your field after a urea application, consider applying a urease inhibitor to your urea ... 4. Conservation or no-till soils tend to have more organic matter, which the urease-producing microbes like, resulting in ...
https://www.ricefarming.com/departments/specialists-speaking/optimize-crop-fertility-with-soil-sampling-urease-inhibitors/
Struvite and Staghorn Calculi Treatment & Management: Medical Therapy, Surgical Therapy, Preoperative DetailsStruvite and Staghorn Calculi Treatment & Management: Medical Therapy, Surgical Therapy, Preoperative Details
Urease inhibitors. The most successful method of oral chemolysis is with urease inhibitors. AHA is the most widely used ... Urease inhibitors with less toxicity may have increased general utility. Also, drugs effective in acidification of the urine ... Staghorn calculi resulting from urease-producing bacteria are best managed with complete surgical removal of the stones. ... Although studies have demonstrated that AHA inhibition of bacterial urease decreases urinary alkalinity and ammonia levels even ...
https://www.medscape.com/answers/439127-170697/what-is-included-in-the-postoperative-monitoring-following-struvite-and-staghorn-calculi-removal
IMSEAR at SEARO: Behaviour of molecular sieve bound urease.
Behaviour of molecular sieve bound urease. Indian Journal of Biochemistry & Biophysics. 1982 Apr; 19(2): 130-4. ...
https://imsear.searo.who.int/handle/123456789/28137
Piperazine-based Semicarbazone Derivatives as Potent Urease Inhibitors: Design, Synthesis, and Bioactivity Screening<...
The urease inhibition activity of these compounds was evaluated using the standard urease enzyme inhibition kit. An MTT assay ... The urease inhibition activity of these compounds was evaluated using the standard urease enzyme inhibition kit. An MTT assay ... The urease inhibition activity of these compounds was evaluated using the standard urease enzyme inhibition kit. An MTT assay ... The urease inhibition activity of these compounds was evaluated using the standard urease enzyme inhibition kit. An MTT assay ...
https://squ.pure.elsevier.com/en/publications/piperazine-based-semicarbazone-derivatives-as-potent-urease-inhib
Urease-mediated destruction of bacteria is specific for Helicobacter urease and results in total cellular disruption -...
Urease-mediated destruction of bacteria is specific for Helicobacter urease and results in total cellular disruption. ... Dive into the research topics of Urease-mediated destruction of bacteria is specific for Helicobacter urease and results in ...
https://research-portal.uws.ac.uk/en/publications/urease-mediated-destruction-of-bacteria-is-specific-for-helicobac/fingerprints/
H.pylori urease A rAg Manufacturer | FrenovoH.pylori urease A rAg Manufacturer | Frenovo
H.pylori urease A rAg is one of the core raw materials used to prepare IVD reagents. HP typing antigen was used in the ... H.pylori Urease A Antigen HP typing antigen was used in the detection of type I (highly carcinogenic) and type II in H. pylori ... Specification of H.pylori Urease A Antigen. Description. Type. Item NO. Source. Application. Recommend to use for. ...
https://www.frenovobio.com/products/h.pylori-urease-a-antigen.html
Urease subunit gamma (ureA) | Technique alternative | 01022739300 - Fytotech
urease subunit gamma; Urease subunit gamma; urease subunit gamma; Urea amidohydrolase subunit gamma ... Urease subunit gamma (ureA) is a recombinant protein expressed in E. coli. The protein can be with or without a His-Tag or ...
http://fytotech.com/best-product/8243-mbs-recombinant/9125-01022739300-clostridium-phytofermentans-urease-subunit-gamma-urea-e-coli-mbs-recombinant-gen1016212-
Review of Maleic-itaconic acid copolymer purported as urease inhibitor and phosphorus enhancer in soils - OAR@ICRISATReview of Maleic-itaconic acid copolymer purported as urease inhibitor and phosphorus enhancer in soils - [email protected]
Chien, S H and Edmeades, D and McBride, R and Sahrawat, K L (2014) Review of Maleic-itaconic acid copolymer purported as urease ... Review of Maleic-itaconic acid copolymer purported as urease inhibitor and phosphorus enhancer in soils ...
http://oar.icrisat.org/7362/
Table 1 - Phenotypic and Genomic Analyses of Burkholderia stabilis Clinical Contamination, Switzerland - Volume 25, Number 6...Table 1 - Phenotypic and Genomic Analyses of Burkholderia stabilis Clinical Contamination, Switzerland - Volume 25, Number 6...
Urease. -. 4. + or -. *Performed using VITEK 2. †From (26,28).. ‡Specific discriminatory test for B. stabilis. ...
https://wwwnc.cdc.gov/eid/article/25/6/17-2119-t1
Urease subunit alpha (Helicobacter pylori 26695) | Protein Target - PubChemUrease subunit alpha (Helicobacter pylori 26695) | Protein Target - PubChem
Protein target information for Urease subunit alpha (Helicobacter pylori 26695). Find diseases associated with this biological ...
https://pubchem.ncbi.nlm.nih.gov/protein/P14916
Urease Test - Principle, Media, Procedure, And Result - World Of BacteriaUrease Test - Principle, Media, Procedure, And Result - World Of Bacteria
Find out the answer to any questions you may have about urease tests and their results. ... The Uses Of The Urease Test. There are lots of uses for a urease test, weve gone over one or two already but below youll find ... Urease Test - Result Interpretation. The procedure of a urease test isnt very complicated. It can be explained through 3 ... Before we get to testing, lets take a second to talk about urease. You may be wondering what urease is and why we test for it ...
https://worldofbacteria.com/urease-test-principle-media-procedure-and-result/
Helicobacter pylori quick urease test - Video | Evidence-Based Medicine GuidelinesHelicobacter pylori quick urease test - Video | Evidence-Based Medicine Guidelines
Helicobacter pylori quick urease test - Video answers are found in the Evidence-Based Medicine Guidelines powered by Unbound ... Helicobacter pylori quick urease test - Video is a topic covered in the Evidence-Based Medicine Guidelines. To view the entire ... Helicobacter pylori quick urease test - Video. (2019). In Evidence-Based Medicine Guidelines. Duodecim Medical Publications ... Helicobacter Pylori Quick Urease Test - Video [Internet]. In: Evidence-Based Medicine Guidelines. Duodecim Medical Publications ...
https://evidence.unboundmedicine.com/evidence/view/EBMG/450350/all/Helicobacter_pylori_quick_urease_test___Video
WEE-TAB UREASE :: Rapid Identification Tests / Bacteria Identification :: Key Scientific ProductsWEE-TAB UREASE :: Rapid Identification Tests / Bacteria Identification :: Key Scientific Products
Prices listed for products not made by KEY SCIENTIFIC are subject to change without notice. Please call for current pricing ...
http://www.keyscientific.com/product.php?productid=84
Classical and variant approaches to synthesis of N-Mannich bases of phenyl hydroxyl ketones, their characterization, urease...Classical and variant approaches to synthesis of N-Mannich bases of phenyl hydroxyl ketones, their characterization, urease...
Classical and variant approaches to synthesis of N-Mannich bases of phenyl hydroxyl ketones, their characterization, urease ...
https://www.morressier.com/o/event/5fc6413703137aa525805a17/article/5fc642112d78d1fec4658149
Further Research | CAUTI Guidelines | Guidelines Libaray | Infection Control | CDCFurther Research | CAUTI Guidelines | Guidelines Libaray | Infection Control | CDC
Irrigation with acidifying solutions or oral urease inhibitors. *Use of methenamine. *Other prevention measures *Use of ...
https://www.cdc.gov/infectioncontrol/guidelines/cauti/further-research.html
Advent calendar 2010 - Science in School
Here, however, are two more unusual activities to use the solution for - investigating the activity of the enzyme urease, and ... Lorenc A (2008) Investigating the action of urease. Science in School 9: 39-44 ...
https://www.scienceinschool.org/article/2015/advent-calendar-2010/
NHANES 1999-2000: Standard Biochemistry Profile & Hormones Data Documentation, Codebook, and Frequencies
Urea is hydrolyzed by urease to form CO2 and ammonia. The ammonia formed then reacts with a-ketoglutarate and NADH in the ...
https://wwwn.cdc.gov/Nchs/Nhanes/1999-2000/LAB18.htm
Isolation and Characterisation on Proteus vulgaris from Mangrove Sediment of Vellar Estuary for Urease Production | Abstract
Urease is a virulence factor found in various pathogenic bacteria. It is essential in colonization of a host organism and in ... Urease is a virulence factor found in various pathogenic bacteria. It is essential in colonization of a host organism and in ... The present study focused on the production of urease by a Proteus vulgarisisolated from a sedi-ment sample of Vellar estuary, ... An excel-lent correlation was found between test and standard urease which evidenced the possibilities of its industrial appli- ...
https://www.eresearchco.com/abstract/isolation-and-characterisation-on-proteus-vulgaris-from-mangrove-sediment-of-vellar-estuary-for-urease-production-64293.html
Gallstones-dissolving capacity of lemon ( Citrus limon ) juice, Herniaria hirsuta L. extract and lemon juice-based natural...Gallstones-dissolving capacity of lemon ( Citrus limon ) juice, Herniaria hirsuta L. extract and lemon juice-based natural...
Urease Inhibitory Activities of some Commonly Consumed Herbal Medicines. *Shabnam Mahernia, K. Bagherzadeh, F. Mojab, M. ...
https://www.semanticscholar.org/paper/Gallstones-dissolving-capacity-of-lemon-%28-Citrus-%29-Chekroune-Benamara/9db848672eda1abc329dc88f73dcc78332f710e3?p2df
Modeling of urease thermal inactivation processes in soybean at high-temperature micronization | Potravinarstvo Slovak...Modeling of urease thermal inactivation processes in soybean at high-temperature micronization | Potravinarstvo Slovak...
Modeling of urease thermal inactivation processes in soybean at high-temperature micronization Authors. * Sergey Zverev Federal ... Soybean, urease, trypsin inhibitor, thermal inactivation, infrared heat treatment Abstract. The use of soybean, in particular ... As a marker in assessing safety of cakes and meals, there is often used urease in forage production. This paper describes the ... The identification of the model coefficients was carried out based on the results of inactivation of urease during heating in ...
https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/940
UreaHelicobacterBacteriaInhibitorsPyloriEnzymesRapid UreaInhibitorProteusEnzyme ureaseAmmoniaInhibition activityActivityInfectionColonizationGenesHistologyExtractsNickelVolatilizationStrainUrinaryOrganismsGammaStruviteIdentificationTestMolecularProductionTemperaturePositiveResultsTestsStandardWild type
Urea20
  • Urease production was inhibited by urea and induced under growth conditions where the availability of nitrogen sources was limiting. (pasteur.fr)
  • Incubation studies were carried out to evaluate the effect of temperature and urea supply level on both the urease activity and the kinetics of N mineralization in two Andisols of Southern Chile. (scielo.cl)
  • The Michaelis constant of the urea-urease system has been found to be approximately 0.003 M urea and the comparable constant defining the phosphate-urease system 0.035 M phosphate. (caltech.edu)
  • When urea is applied to soil, microbes in the soil release an enzyme called urease, which catalyzes the breakdown of urea to ammonium. (ricefarming.com)
  • If you have trouble getting water across your field after a urea application, consider applying a urease inhibitor to your urea - especially if the time between urea application and a flush or flood is greater than three days. (ricefarming.com)
  • Specifically, the presence of urease-producing bacteria, including Ureaplasma urealyticum and Proteus species (most common), Staphylococcus species, Klebsiella species, Providencia species, and Pseudomonas species, leads to the hydrolysis of urea into ammonium and hydroxyl ions. (medscape.com)
  • Urease subunit gamma (ureA) is a recombinant protein expressed in E. coli. (fytotech.com)
  • If there is an infection, the urease that is created by H. Pylori will hydrolyze the urea and form ammonia, much like in the aforementioned test, but this time will also produce labeled bicarbonate which is breathed out as carbon dioxide. (worldofbacteria.com)
  • This test can differentiate which, if any, organisms are present by how well urea is hydrolyzed by urease. (worldofbacteria.com)
  • An exce l- lent correlation was found between test and st andard urease which evidenced the possibilities of its industrial appl i- cations as urea diagnosis and reduction. (eresearchco.com)
  • Biopsy specimens were immediately placed onto rapid urease test kit containing urea and an indicator. (ijsurgery.com)
  • Though there is no established gold standard for the diagnosis of HP infection, several methods including serology, urea breath test, rapid urease test, faecal antigen test, culture from biopsy, and histological evaluation have good accuracy [ 4 ]. (hindawi.com)
  • but urease can only hydrolyze urea. (articlesfactory.com)
  • Enzima que cataliza la conversión de la urea y agua en dióxido de carbono y amonio. (bvsalud.org)
  • Urease is an enzyme containing a dinuclear nickel active center responsible for the hydrolysis of urea into carbon dioxide and ammonia. (bvsalud.org)
  • The reason behind the discrepancy in urea decomposition mechanisms between inorganic models and urease is still unknown. (bvsalud.org)
  • Both mimetic complexes produced ammonia from urea, (1) and (2), were ten- and four-fold slower than urease, respectively. (bvsalud.org)
  • As a mechanistic explanation for the detection of P. mirabilis and K. pneumoniae, urease enzymes secreted from the P. mirabilis and K. pneumoniae hydrolyze urea to produce ammonia (NH3), which increases the pH value of the reaction environment and leads to deprotonation from anthocyanins. (bvsalud.org)
  • For example, urease, a famous nickel-containing enzyme that hydrolyzes urea into carbon dioxide and ammonia (a key step in the biogeochemical nitrogen cycle on Earth), is inhibited by Ag+ cations, but the molecular mechanism of silver's action is poorly understood. (bvsalud.org)
  • The Au stars functionalized with 11-mercaptoundecanoic acid and immobilized with urease showed improved urea-sensing behavior in comparison to pure gold disk in attenuated total reflection surface plasmon resonance (ATR SPR) mode. (archives-ouvertes.fr)
Helicobacter8
  • The C. perfringens enzyme was highly similar to the ureases of other bacteria and cross-reacted with antibodies raised against the urease purified from Helicobacter pylori. (pasteur.fr)
  • It also tests for Helicobacter Pylori by monitoring urease activity. (worldofbacteria.com)
  • It can also be helpful in identifying Cryptococcus spp (an infection associated with immunosuppressive individuals) Helicobacter Pylori (stomach infection) and plenty of other types of bacteria that create urease enzymes that can make you sick. (worldofbacteria.com)
  • Helicobacter pylori quick urease test - Video is a topic covered in the Evidence-Based Medicine Guidelines . (unboundmedicine.com)
  • Evidence Central , evidence.unboundmedicine.com/evidence/view/EBMG/450350/all/Helicobacter_pylori_quick_urease_test___Video. (unboundmedicine.com)
  • The aim of our study was to determine the prevalence of active Helicobacter pylori ( H. pylori) infection by retrospective review of patient records using only rapid urease test reports which gives us the magnitude of the H. pylori prevalence. (ijsurgery.com)
  • Makaju R, Tamang MD, Sharma Y. Comparative study on a homemade rapid urease test with gastric biopsy for diagnosis of Helicobacter pylori infection. (ijsurgery.com)
  • Urease enzyme has a crucial role in the persistent habitation of Helicobacter pylori (H. pylori) that induces gastrointestinal diseases, in particular gastritis, duodenal, peptic ulcer, and gastric cancer. (sid.ir)
Bacteria6
  • Although many bacteria are ureolytic, and in some cases urease acts as a virulence factor, the urease phenotype has not been analyzed in the anaerobic pathogen Clostridium perfringens. (pasteur.fr)
  • Staghorn calculi resulting from urease-producing bacteria are best managed with complete surgical removal of the stones. (medscape.com)
  • Background: An enzyme called urease assists highly pathogenic bacteria in colonizing and maintaining themselves. (elsevier.com)
  • Urease is a virulence factor found in various pathogenic bacteria. (eresearchco.com)
  • recurrent UTI (urease forming bacteria: proteus / klebsiella). (studystack.com)
  • The enzyme urease produces ammonia and enables bacteria to adapt to an acidic environment. (umf.org.nz)
Inhibitors3
  • The most successful method of oral chemolysis is with urease inhibitors. (medscape.com)
  • Conclusion: The results showed that semicarbazone derivatives could be highly effective as urease inhibitors. (elsevier.com)
  • With the highest concentration of nitrogen available in a granule, SUPERU contains urease and nitrification inhibitors to guard crops from denitrification, leaching and volatilization. (kochagronomicservices.ca)
Pylori4
  • Positive test for H. pylori was confirmed by the change in color of the medium from yellow to pink or red and when the rapid urease test was positive, patient was considered as H. pylori positive. (ijsurgery.com)
  • After being aware of the prevalence of H. pylori by early detection of active H. pylori infections, rapid urease test serves only as a rapid screening tool in dyspeptic symptoms of patients with acid peptic disease than all other tests. (ijsurgery.com)
  • the antrum and one from the body, were immediately tested for H. pylori using the rapid urease test (CLO Test), whilst the remaining two specimens were submitted for histological evaluation. (ispub.com)
  • The presence of H. pylori in antrum biopsies was confirmed by rapid urease test and/or histopathological examination. (who.int)
Enzymes2
  • Accordingly, inhibiting urease enzymes has been shown to be a promising strategy for preventing ureolytic bacterial infections. (elsevier.com)
  • There are two enzymes, urease, and carbonic anhydrase, that play an important role in the MICP process. (frontiersin.org)
Rapid Urea2
  • The biopsy samples were examined using a rapid urease test (RUT) and histopathology study. (ajol.info)
  • The Rapid Urease Test - Some gastroenterologists will keep a specific broth handy during the biopsy procedure. (canine-megaesophagus.com)
Inhibitor5
  • I think most rice farmers in Texas use a urease inhibitor, and I think this a good practice because of Southeast Texas' erratic weather. (ricefarming.com)
  • AHA is the most widely used irreversible inhibitor of bacterial urease. (medscape.com)
  • But with powerful urease inhibitor technology like ANVOL ® and AGROTAIN ® , growers can extend their nitrogen availability and optimize their yield. (kochagronomicservices.ca)
  • AGROTAIN nitrogen stabilizer is the most research-proven urease inhibitor on the market. (kochagronomicservices.ca)
  • ANVOL™ nitrogen stabilizer from Koch Agronomic Services will deliver long-lasting urease inhibitor technology in a wide range of pH levels. (kochagronomicservices.ca)
Proteus2
  • The present study focused on the production of urease by a Proteus vulgaris isolated from a sed i- ment sample of Vellar estuary, Porto Novo, Tamil Nadu, India. (eresearchco.com)
  • Enterobacteriaceae species especially urease positive ones, Proteus mirabilis (P. mirabilis) and Klebsiella pneumoniae (K. pneumoniae), show resistance to antibiotics and cause respiratory and urinary tract infections. (bvsalud.org)
Enzyme urease1
  • Here, however, are two more unusual activities to use the solution for - investigating the activity of the enzyme urease, and microscale chemistry experiments. (scienceinschool.org)
Ammonia2
  • Although studies have demonstrated that AHA inhibition of bacterial urease decreases urinary alkalinity and ammonia levels even in the presence of infection, 20% of patients experience associated adverse effects. (medscape.com)
  • A new enzymatic assay, based on photometric detection of ammonia with ninhydrin, was developed to study urease activity. (umf.org.nz)
Inhibition activity2
  • Antioxidant and urease inhibition activity of methanol extract of Hibiscus schizopetalus (Mast) Hook. (phytojournal.com)
  • The urease inhibition activity of these compounds was evaluated using the standard urease enzyme inhibition kit. (elsevier.com)
Activity15
  • Our results showed that urease activity was higher in Piedras Negras soil (PNS) than Freiré soil (FS) at 24 and 48 h, and the enzyme was activated when the temperature increased from 5 to 21 °C. Urease activity followed a simple Michaelis-Menten kinetics. (scielo.cl)
  • In FS, urease activity was the highest at 24 h, and it decreased at 48 h as a consequence of substrate depletion. (scielo.cl)
  • Importance of urease activity on pathogenic differences among Brucella species was evaluated. (scirp.org)
  • In cell-free extracts, the B . suis urease showed 12 times greater specific activity than the B. melitensis urease. (scirp.org)
  • and urease activity has no influence on Brucella infection using an IP route. (scirp.org)
  • E. Sanders and J. Warner, "A Study of Urease Activity in Cells of the Genus Brucella," Journal of Bacteriology, Vol. 62, No. 5, 1951, pp. 591-598. (scirp.org)
  • The maximum activity of extracts (HFE, HLE) was observed at 150 µg/ml (80.70% and 75.2%) respectively, whereas ascorbic acid exhibited 81.02% urease inhibition at the same concentrations. (phytojournal.com)
  • HFE extract showed maximum urease activity with percentage inhibition of 55.5%, IC50 80.1 ± 0.87 mg/ml. (phytojournal.com)
  • Due to its enzymatic activity, urease has quite a toxic effect on humans and so you're going to want it in your system, ideally not at all, but if it is there, you're going to want it out as quickly as possible. (worldofbacteria.com)
  • Based on the maximum enzyme activity on urease slant and plate assay as well as quantitative biochemical enzyme assay, most potential strains were chosen for further stu dies. (eresearchco.com)
  • The similar dependences of urease activity on grain temperature are invariant to infrared heating (irradiation and time) regimes, but their nature is affected by the initial moisture content. (potravinarstvo.com)
  • Activity of urease in soybean meal. (potravinarstvo.com)
  • 15 medicinal plant extracts were examined against Jack bean Urease activity by Berthelot reaction. (sid.ir)
  • CeO2-x nanorods with intrinsic urease-like activity. (mpg.de)
  • The relationship between dental caries status and dental plaque urease activity. (bvsalud.org)
Infection1
  • Struvite stones are also known as triple-phosphate (3 cations associated with 1 anion), infection (or infection-induced), phosphatic, and urease stones. (medscape.com)
Colonization2
  • D. M. Monack and S. Falkow, "Cloning of Bordetella Bronchiseptica Urease Genes and Analysis of Colonization by a Urease-Negative Mutant Strain in a Guinea-Pig Model," Molecular Microbiology, Vol. 10, No. 3, 1993, pp. 545-553. (scirp.org)
  • Well, urease is essential in the colonization of a host organism and it's also incredibly important in the maintenance of bacterial cells in tissues. (worldofbacteria.com)
Genes1
  • In this study, approximately 2% of C. perfringens strains, representing the principal biotypes, were found to harbor the urease structural genes, ureABC, and these were localized on large plasmids that often encode, in addition, the lethal epsilon or iota toxins or the enterotoxin. (pasteur.fr)
Histology1
  • These tests include the urease test (for example, the CLO test) and histology (looking at the tissue under a microscope). (medlineplus.gov)
Extracts1
  • The present study was aimed at measuring the antioxidant and urease inhibition capacities of methanolic extracts of flower and leaves (HFE and HLE) of Hibiscus schizopetalus (Mast) Hook (Malvaceae) an ornamental plant of this region. (phytojournal.com)
Nickel2
  • Nickel (II) complexes as functional urease mimics. (albion.edu)
  • To evaluate this factor, we synthesized two bis-nickel complexes, [Ni2L(OAc)] (1) and [Ni2L(Cl)(Et3N)2] (2), based on the Trost bis-Pro-Phenol ligand (L) and encompassing different ligand labilities with coordination geometries similar to the active site of jack bean urease. (bvsalud.org)
Volatilization1
  • 4. Conservation or no-till soils tend to have more organic matter, which the urease-producing microbes like, resulting in greater volatilization. (ricefarming.com)
Strain1
  • Genome Sequence of a Urease-Positive Campylobacter lari Strain. (cdc.gov)
Urinary1
  • Research on urease inhibitions from natural source yielded several imperative therapeutic drugs that are useful in gastric and urinary tract infections. (phytojournal.com)
Organisms1
  • Urease-producing organisms are listed in Etiology. (medscape.com)
Gamma1
  • Immobilization of glucose oxidase and urease in hydrogels of 2-hydroxyethyl methacrylae, and N-vinyl pyrrolidone (NVP) was achieved by irradiation (using UV kand gamma-rays). (metu.edu.tr)
Struvite1
  • Escherichia coli does not produce urease and is not associated with struvite stone formation. (medscape.com)
Identification1
  • The identification of the model coefficients was carried out based on the results of inactivation of urease during heating in the flux of infrared radiation. (potravinarstvo.com)
Test4
  • You may be wondering what urease is and why we test for it. (worldofbacteria.com)
  • The sooner you can test and know that urease is present, the sooner you can get the treatment needed to sort it. (worldofbacteria.com)
  • Principle Of The Urease Test. (worldofbacteria.com)
  • There are lots of uses for a urease test, we've gone over one or two already but below you'll find a more comprehensive list of reasons we may use one of the tests we've just learned about. (worldofbacteria.com)
Molecular2
  • IMSEAR at SEARO: Behaviour of molecular sieve bound urease. (who.int)
  • Iyengar L, Bajpai P, Prabhakara Rao AV. Behaviour of molecular sieve bound urease. (who.int)
Production2
  • As a marker in assessing safety of cakes and meals, there is often used urease in forage production. (potravinarstvo.com)
  • Other adhesive factors include its pili and fimbriae.Its production of urease and hemolysins are additional pathogenic properties. (medscape.com)
Temperature1
  • This paper describes the results of thermal inactivation of urease in soybean during the process of high-temperature micronization (heating of grain in the flux of infrared radiation). (potravinarstvo.com)
Positive2
  • This represents the first report of a plasmid-encoded urease in a gram-positive bacterium. (pasteur.fr)
  • Which are Urease positive? (proprofs.com)
Results1
  • Thus, our results indicate that the formation of an outer-sphere complex in the urease analogues might be the reason urease performs a different mechanism. (bvsalud.org)
Tests2
  • We have developed natural indicator-incorporated colorimetric urease tests with a naked eye and smartphone readout to rapidly, sensitively and economically detect P. mirabilis and K. pneumoniae. (bvsalud.org)
  • We claim that natural indicator-incorporated rapid urease tests providing colorimetric readout evaluated by the human eye and smartphone imaging processing has great potential in practical use and they can be implemented in clinics. (bvsalud.org)
Standard1
  • The percentage urease inhibition of standard Thiourea was found to be 98.2%, IC50 88.2 ± 0.01 mg/ml. (phytojournal.com)
Wild type1
  • The wild type and the urease deficient strains of B. suis did not differ from each other in terms of recovery from spleen or liver. (scirp.org)
Struvite and Staghorn Calculi Treatment & Management: Medical Therapy, Surgical Therapy, Preoperative Details
Struvite and Staghorn Calculi Treatment & Management: Medical Therapy, Surgical Therapy, Preoperative Details (medscape.com)
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