An enzyme that catalyzes the HYDROLYSIS of the N-glycosidic bond between sugar phosphate backbone and URACIL residue during DNA synthesis.
A family of DNA repair enzymes that recognize damaged nucleotide bases and remove them by hydrolyzing the N-glycosidic bond that attaches them to the sugar backbone of the DNA molecule. The process called BASE EXCISION REPAIR can be completed by a DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE which excises the remaining RIBOSE sugar from the DNA.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
Proteins involved in the transport of NUCLEOTIDES across cellular membranes.
Uracil nucleotides which contain deoxyribose as the sugar moiety.
5-Bromo-2,4(1H,3H)-pyrimidinedione. Brominated derivative of uracil that acts as an antimetabolite, substituting for thymine in DNA. It is used mainly as an experimental mutagen, but its deoxyriboside (BROMODEOXYURIDINE) is used to treat neoplasms.
Enzymes of the transferase class that catalyze the transfer of a pentose group from one compound to another.
Nitrogen mustard derivative of URACIL. It is a alkylating antineoplastic agent that is used in lymphatic malignancies, and causes mainly gastrointestinal and bone marrow damage.
5'-Uridylic acid. A uracil nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.
A pyrimidine base that is a fundamental unit of nucleic acids.
The removal of an amino group (NH2) from a chemical compound.
An enzyme that catalyzes the transfer of ribose from uridine to orthophosphate, forming uracil and ribose 1-phosphate.
An oxidoreductase involved in pyrimidine base degradation. It catalyzes the catabolism of THYMINE; URACIL and the chemotherapeutic drug, 5-FLUOROURACIL.
Congener of FLUOROURACIL with comparable antineoplastic action. It has been suggested especially for the treatment of breast neoplasms.
A pyrimidine nucleoside formed in the body by the deamination of CYTARABINE.
Occurs in seeds of Brassica and Crucifera species. Thiouracil has been used as antithyroid, coronary vasodilator, and in congestive heart failure although its use has been largely supplanted by other drugs. It is known to cause blood dyscrasias and suspected of terato- and carcinogenesis.
An autosomal recessive disorder affecting DIHYDROPYRIMIDINE DEHYDROGENASE and causing familial pyrimidinemia. It is characterized by thymine-uraciluria in homozygous deficient patients. Even a partial deficiency in the enzyme leaves individuals at risk for developing severe 5-FLUOROURACIL-associated toxicity.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
An enzyme that removes THYMINE and URACIL bases mispaired with GUANINE through hydrolysis of their N-glycosidic bond. These mispaired nucleotides generally occur through the hydrolytic DEAMINATION of 5-METHYLCYTOSINE to thymine.
Pyrimidines with a RIBOSE attached that can be phosphorylated to PYRIMIDINE NUCLEOTIDES.
2'-Deoxyuridine. An antimetabolite that is converted to deoxyuridine triphosphate during DNA synthesis. Laboratory suppression of deoxyuridine is used to diagnose megaloblastic anemias due to vitamin B12 and folate deficiencies.
Orotidine-5'-phosphate carboxy-lyase. Catalyzes the decarboxylation of orotidylic acid to yield uridylic acid in the final step of the pyrimidine nucleotide biosynthesis pathway. EC 4.1.1.23.
The enzyme catalyzing the formation of orotidine-5'-phosphoric acid (orotidylic acid) from orotic acid and 5-phosphoribosyl-1-pyrophosphate in the course of pyrimidine nucleotide biosynthesis. EC 2.4.2.10.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Inorganic salts of sulfurous acid.
A family of 6-membered heterocyclic compounds occurring in nature in a wide variety of forms. They include several nucleic acid constituents (CYTOSINE; THYMINE; and URACIL) and form the basic structure of the barbiturates.
A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.
An enzyme that catalyzes the deamination of cytidine, forming uridine. EC 3.5.4.5.
A nutritional condition produced by a deficiency of FOLIC ACID in the diet. Many plant and animal tissues contain folic acid, abundant in green leafy vegetables, yeast, liver, and mushrooms but destroyed by long-term cooking. Alcohol interferes with its intermediate metabolism and absorption. Folic acid deficiency may develop in long-term anticonvulsant therapy or with use of oral contraceptives. This deficiency causes anemia, macrocytic anemia, and megaloblastic anemia. It is indistinguishable from vitamin B 12 deficiency in peripheral blood and bone marrow findings, but the neurologic lesions seen in B 12 deficiency do not occur. (Merck Manual, 16th ed)
5-Hydroxymethyl-6-methyl- 2,4-(1H,3H)-pyrimidinedione. Uracil derivative used in combination with toxic antibiotics to lessen their toxicity; also to stimulate leukopoiesis and immunity. Synonyms: pentoksil; hydroxymethylmethyluracil.
A purine base and a fundamental unit of ADENINE NUCLEOTIDES.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Inhibitor of DNA replication in gram-positive bacteria.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Organic compounds that contain phosphorus as an integral part of the molecule. Included under this heading is broad array of synthetic compounds that are used as PESTICIDES and DRUGS.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
Cancer or tumors of the MAXILLA or upper jaw.
The solid substance formed by the FREEZING of water.
Nanometer-scale wires made of materials that conduct electricity. They can be coated with molecules such as antibodies that will bind to proteins and other substances.
A membrane or barrier with micrometer sized pores used for separation purification processes.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.
Information intended for potential users of medical and healthcare services. There is an emphasis on self-care and preventive approaches as well as information for community-wide dissemination and use.
Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.
Differences of opinion or disagreements that may arise, for example, between health professionals and patients or their families, or against a political regime.

Base excision repair of oxidative DNA damage activated by XPG protein. (1/1786)

Oxidized pyrimidines in DNA are removed by a distinct base excision repair pathway initiated by the DNA glycosylase--AP lyase hNth1 in human cells. We have reconstituted this single-residue replacement pathway with recombinant proteins, including the AP endonuclease HAP1/APE, DNA polymerase beta, and DNA ligase III-XRCC1 heterodimer. With these proteins, the nucleotide excision repair enzyme XPG serves as a cofactor for the efficient function of hNth1. XPG protein promotes binding of hNth1 to damaged DNA. The stimulation of hNth1 activity is retained in XPG catalytic site mutants inactive in nucleotide excision repair. The data support the model that development of Cockayne syndrome in XP-G patients is related to inefficient excision of endogenous oxidative DNA damage.  (+info)

Synthesis of bacteriophage phi6 double-stranded ribonucleic acid. (2/1786)

Uracil was incorporated into all three bacteriophage phi6 dsRNA segments throughout the infection cycle; the rates of incorporation into each of the three segments were approx. constant for the first 15 to 20 min and then increased rapidly until 50 min after infection. The medium and small dsRNA segments were produced in greater amounts than the large dsRNA segment at all times in the infection cycle. Inhibition of host RNA and protein synthesis with rifampin and chloramphenicol revealed that virus dsRNA synthesis immediately after infection was independent of either host function.  (+info)

Phase I study of eniluracil, a dihydropyrimidine dehydrogenase inactivator, and oral 5-fluorouracil with radiation therapy in patients with recurrent or advanced head and neck cancer. (3/1786)

5-Fluorouracil (5-FU) is an effective enhancer of radiation therapy (RT) in head and neck cancers. Due to rapid, predominantly hepatic metabolism by dihydropyrimidine dehydrogenase (DPD) and suggested clinical benefit from prolonged drug exposure, 5-FU is commonly given by continuous infusion. Eniluracil is a novel DPD-inactivator designed to prolong the half-life of 5-FU and provide sustained plasma concentrations of 5-FU with oral dosing. We conducted a Phase I study of the safety and efficacy of eniluracil given with oral 5-FU in patients receiving concurrent RT for recurrent or advanced squamous cell carcinomas of the head and neck. Thirteen patients with recurrent, metastatic, or high-risk (defined as an expected 2-year survival rate of <10%) head and neck cancer were enrolled and treated with concomitant chemoradiotherapy on an every-other-week schedule. Eniluracil at a fixed dose [20 mg twice a day (BID)] was given for 7 consecutive days (days 1-7). 5-FU and RT were given on 5 consecutive days (days 2-6). One patient was treated with once-daily RT (2.0 Gy fractions). The remaining patients received hyperfractionated RT (1.5-Gy fractions BID). The initial dose of 5-FU was 2.5 mg/m2 given BID. Dose escalation in patient cohorts was scheduled at 2.5-mg/m2 increments, with intrapatient dose escalation permitted. Lymphocyte DPD activity and serum 5-FU and uracil concentrations were monitored during two cycles. DPD activity was completely or nearly completely inactivated in all patients. Sustained, presumed therapeutic concentrations of 5-FU were observed at a dose of 5.0 mg/m2 given BID. Cumulative dose-limiting myelosuppression (both neutropenia and thrombocytopenia) was observed during the fourth and fifth cycles following administration of 5.0 mg/m2 5-FU BID. One patient died of neutropenic sepsis during cycle 4. Other late cycle toxicities included diarrhea, fatigue, and mucositis. Grade 3 mucositis was observed in 4 patients, but no grade 4 mucositis or grade 3 or 4 dermatitis was observed. A second patient death occurred during cycle 1 of treatment. No specific cause of death was identified. The study was subsequently discontinued. Cumulative myelosupression was the significant dose-limiting toxicity of oral 5-FU given with the DPD-inactivator eniluracil on an every-other-week schedule. Clinical radiation sensitization was not observed, based on the absence of dose-limiting mucositis and dermatitis. Alternative dosing schedules need to be examined to determine the most appropriate use of eniluracil and 5-FU as radiation enhancers.  (+info)

Base pairing of anhydrohexitol nucleosides with 2,6-diaminopurine, 5-methylcytosine and uracil asbase moiety. (4/1786)

Hexitol nucleic acids (HNAs) with modified bases (5-methylcytosine, 2,6-diaminopurine or uracil) were synthesized. The introduction of the 5-methylcytosine base demonstrates that N -benzoylated 5-methylcytosyl-hexitol occurs as the imino tautomer. The base pairing systems (G:CMe, U:D, T:D and U:A) obey Watson-Crick rules. Substituting hT for hU, hCMefor hC and hD for hA generally leads to increased duplex stability. In a single case, replacement of hC by hCMedid not result in duplex stabilization. This sequence-specific effect could be explained by the geometry of the model duplex used for carrying out the thermal stability study. Generally, polypurine HNA sequences give more stable duplexes with their RNA complement than polypyrimidine HNA sequences. This observation supports the hypothesis that, besides changes in stacking pattern, the difference in conformational stress between purine and pyrimidine nucleosides may contribute to duplex stability. Introduction of hCMeand hD in HNA sequences further increases the potential of HNA to function as a steric blocking agent.  (+info)

Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents. (5/1786)

The effect of alkyltrimethylammonium ions on the thermostability of natural and modified DNA duplexes has been investigated. We have shown that the use of tetramethylammonium ions TMA+along with the chemical modification of duplexes allow the fine adjustment of T m and the possibility of obtaining several duplex systems with varied isostabilizedtemperatures, some of which show greater stability than those of natural DNA. This approach could be very useful for DNA sequencing by hybridization.  (+info)

Five caffeine metabolite ratios to measure tobacco-induced CYP1A2 activity and their relationships with urinary mutagenicity and urine flow. (6/1786)

To choose a sensitive protocol to discriminate populations exposed and not exposed to inducers, five urinary metabolite ratios (MRs) [MR1 (17X + 17U)/137X, MR2 (5-acetylamino-6-formylamino-3-methyluracil [AFMU] + 1X + 1U)/17U, MR3 (17X/137X), MR4 (AFMU + 1X + 1U + 17X + 17U)/137X, and MR5 (AFMU + 1X + 1U)/17X] were calculated in 4-5 h and 0-24 h urine samples after caffeine intake. One hundred twenty-five healthy volunteers (59 nonsmokers and 66 smokers) were included in the study. All ratios showed a log-normal distribution. MR2 in the two time intervals was the only ratio nondependent on the urine flow. Differences between nonsmokers and smokers could be detected with all ratios at 4-5 h. However, only MR2 and, to a lesser extent, MR5 allowed the discrimination of higher cytochrome P450 1A2 (CYP1A2) activity in smokers in the 0-24 h sample. Although smokers had increased urinary mutagenicity in relation to nonsmokers, a significant association between MRs and urine mutagenicity was observed only with MR2 in the 4-5 h interval; this ratio/time schedule being that of higher association with tobacco consumption. The most flow-dependent ratios, MR1, MR3, and MR4, were closely correlated with each other at the two intervals. The flow dependency profile of each ratio may explain their different power to indicate both tobacco exposure and tobacco-derived mutagenicity. In conclusion, MR2 in the period of 4-5 h after caffeine intake seems preferable, especially at high urine flow rates.  (+info)

Uracil-induced down-regulation of the yeast uracil permease. (7/1786)

In Saccharomyces cerevisiae the FUR4-encoded uracil permease catalyzes the first step of the pyrimidine salvage pathway. The availability of uracil has a negative regulatory effect upon its own transport. Uracil causes a decrease in the level of uracil permease, partly by decreasing the FUR4 mRNA level in a promoter-independent fashion, probably by increasing its instability. Uracil entry also triggers more rapid degradation of the existing permease by promoting high efficiency of ubiquitination of the permease that signals its internalization. A direct binding of intracellular uracil to the permease is possibly involved in this feedback regulation, as the behavior of the permease is similar in mutant cells unable to convert intracellular uracil into UMP. We used cells impaired in the ubiquitination step to show that the addition of uracil produces rapid inhibition of uracil transport. This may be the first response prior to the removal of the permease from the plasma membrane. Similar down-regulation of uracil uptake, involving several processes, was observed under adverse conditions mainly corresponding to a decrease in the cellular content of ribosomes. These results suggest that uracil of exogenous or catabolic origin down-regulates the cognate permease to prevent buildup of excess intracellular uracil-derived nucleotides.  (+info)

Only one of the charged amino acids located in membrane-spanning regions is important for the function of the Saccharomyces cerevisiae uracil permease. (8/1786)

The transport of uracil into the yeast Saccharomyces cerevisiae is mediated by uracil permease, a specific co-transporter encoded by the FUR4 gene. Uracil permease is a multispan membrane protein that is delivered to the plasma membrane via the secretory pathway. Experimental results led to the proposal of a two-dimensional model of the protein's topology. According to this model, the membrane domain of Fur4p contains three charged amino acid residues (Glu-243, Lys-272 and Glu-539) that are conserved in the members of the FUR family of yeast transporters. We have previously shown that a mis-sense mutation leading to the replacement of Lys-272 by Glu severely impairs the function of uracil permease. In the present paper, the role of the three charged residues present in the membrane-spanning regions of Fur4p was further investigated by using site-directed mutagenesis. The variant permeases were correctly targeted to the plasma membrane and their stabilities were similar to that of the wild-type permease. The effect of the mutations was studied by measuring the uptake constants for uracil on whole cells and equilibrium binding parameters on plasma membrane-enriched fractions. We found no evidence for ionic interaction between either of the glutamic residues in transmembrane segments 3 and 9 and the lysine residue in transmembrane segment 4. Of the three charged residues, only Lys-272 was important for the transport activity of the transporter. Its replacement by Ala, Glu or even Arg strongly impaired both the binding and the translocation of uracil.  (+info)

BioAssay record AID 359724 submitted by ChEMBL: Antiparasitic activity against Toxoplasma gondii infected in HFF cells at 0.1 uM after 48 hrs by [3H]uracil incorporation assay relative to control.
Uracil je jedna od četiri nukleobaza RNK, koja zamenjuje timin koji se nalazi u DNK. Kao i timin, uracil može da formira bazni par sa adeninom pomoću dve vodonične veze, ali mu nedostaje metil grupa koja postoji u timinu. Uracil će, za razliku od timina, lakše da se degeneriše u citozin. Uracil se vrlo retko može naći u DNK. ...
Definition of uracil in the Definitions.net dictionary. Meaning of uracil. What does uracil mean? Information and translations of uracil in the most comprehensive dictionary definitions resource on the web.
Uracil is a pyrimidine nucleobase, a heterocyclic aromatic organic compound. Uracil is a planar, unsaturated compound that has the ability to absorb light. Found in RNA, uracil base pairs with adenine through hydrogen bonding and is replaced by thymine in DNA. Uracil can base pair with any of the bases depending on how the molecule arranges itself on the helix, but readily pairs with adenine. Uracil can also bind with a ribose sugar to form a ribonucleoside, uridine. When a phosphate attaches to uridine, uridine 5-monophosphate is produced. Uracil also recycles itself to form nucleotides by undergoing a series of phophoribosyltransferase reactions. Degradation of uracil produces substrates, aspartate, carbon dioxide, and ammonia. Uridine can be phosphorylated with phosphoric acid groups, creating Uridine emonophosphate (UMP), Uridine diphosphate (UDP) or Uridine triphosphate (UTP). ...
TY - GEN. T1 - Interaction of electrons and protons with the RNA-base uracil. AU - Hanel, G.. AU - Gstir, B.. AU - Denifl, S.. AU - Lindinger, C.. AU - Matejcik, S.. AU - Scheier, P.. AU - Limtrakul, J.. AU - Probst, M.. AU - Coupier, B.. AU - Farizon, B.. AU - Farizon, M.. AU - Deutsch, H.. AU - Becker, K.. AU - Märk, T. D.. PY - 2002. Y1 - 2002. N2 - The determination of accurate appearance energy was analyzed for the interaction of electrons and protons with the RNA-base Uracil using rare gases. The electron energy range employed for the electron attachement studies was from about zero up to 15 eV. The slow electrons were colliding cellular RNA compound uracil. It was generally considered that the types of primary damage in DNA by ionizing radiation led to the most significant biological effect. The onset of the first resonance for the anions appeared at the relatively high electron of about 5eV for CN - and at 9eV for the O, which was followed by two resonances with the maximum at ...
Alfa Chemistry is the worlds leading provider for special chemicals. We offer qualified products for 4628-39-1(URACIL-4-ACETIC ACID),please inquire us for 4628-39-1(URACIL-4-ACETIC ACID).
RNA is typically a single strand of nucleotides that is folded back onto itself. It is composed of the compounds Adenine, Uracil, Guanine, and Cytosine. This product includes one Adenine and one Uracil cufflink.
Uracil (U) is one of the four bases found in RNA. It is a pyrimidine base and it never presents in DNA instead of thymine [1] ...
16908-84-2 - Uracil labeled with tritium - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Creative-Proteomics offer cas 66-22-8 URACIL (2-13C, 99%). We are specialized in manufacturing Stabel Isotope Labeled Analytical Standard products.
16S rRNA (uracil1498-N3)-methyltransferaseS-adenosyl-L-methionine + uracil1498 in 16S rRNA = S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA ...
1997-2005 Healthboard.com. Healthboard.com is a purely informational website, and should not be used as a substitute for professional legal, medical or technical advice. ...
አር ኤን ኤ ( RNA ) እንደ ዲ ኤን ኤ ( DNA ) ከኒክሉኢክ አሲድ ( Nucleic acid ) የተሠራ ነው። እንደ ዲ ኤን ኤ አራት ቤዝ (base) አለው። እነሱም አዴናዪን ( A, adenine) ፤ ዩራሲል ( U, uracil ) ( ዲ ኤን ኤ ግን በዩራሲል ፋንታ ታያሚን ( T, thymine ) ነው ያለው) ጓኒን ( G, guanine ) እና ሳይቶሲን ( C, Cytosine )። ኑክሌይክ አሲዶች ደግሞ ሶስት መሰረታዊ አካላቶች አላቸው። ቤዝ፡ ሱካሩ ( sugar group ) እና ፎስፌት ግሩፑ ( the phosphate group ) ናቸው። አር ኤን ኤን ከዲ ኤን ኤ የሚለየዉ ሌላው ነገር የአር ኤን ኤ ስኳር ሁለተኛ ካርቦን ሀይድሮክሲል ( hydroxyl group (-OH )) ሲኖረው ዲ ኤን ኤ ግን ያለው ኤች ( H ) ብቻ ነው። ይህም አር ኤን ኤን በጣም ተለካካፊ ( reactive ) አድርጎታል። ...
A requirement for carbon dioxide or bicarbonate has been demonstrated for the growth and survival of the Jensen, JA-1, and JA-2 sarcomas in vitro. When the cells were grown in the presence of C14O2, isotope was isolated in incorporated aspartic acid, glutamic acid, and proline as well as in the purines and pyrimidines. Although the relative specific activity of the purines and thymine was approximately the same, it was much lower in cytosine and slightly higher in uracil. From this information carbonate-sparing experiments were performed, and uracil partially spared this requirement, whereas the other organic bases were inactive.. ...
Tohoku University scientists have found that human editing enzymes are likely behind a type of mutation in the COVID-19 virus that stimulates the release of pro-inflammatory molecules called cytokines by immune cells in the body.
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10:19, 5 June 2007‎ Aschlafly (Talk , contribs)‎ . . (239 bytes) (+239)‎ . . (New page: A represents either: :Adenine, which is a purine base in DNA and RNA; A pairs with thymine in DNA or uracil in RNA. :Adenosine, which is the the nucleoside c...) ...
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Hydrolytic deamination of DNA cytosine residues results in U/G mispairs, pre-mutagenic lesions threatening long-term genetic stability. Hence, DNA uracil repair is ubiquitous throughout all extant life forms and base excision repair, triggered by a uracil DNA glycosylase (UDG), is the mechanistic paradigm adopted, as it seems, by all bacteria and eukaryotes and a large fraction of archaea. However, members of the UDG superfamily of enzymes are absent from the extremely thermophilic archaeon Methanothermobacter thermautotrophicus Delta H. This organism, as a hitherto unique case, initiates repair by direct strand incision next to the DNA-U residue, a reaction catalyzed by the DNA uridine endonuclease Mth212, an ExoIII homologue. To elucidate the detailed mechanism, in particular to identify the molecular partners contributing to this repair process, we reconstituted DNA uracil repair in vitro from only four purified enzymes of M. thermautotrophicus Delta H. After incision at the 5-side of a ...
RNA was isolated from subribosomal particles of the malaria parasite Plasmodium knowlesi. The nucleotide composition (mole fraction) of the principal species was obtained (S-rRNA, 0.295A, 0.36U, 0.25G, 0.105C: L-rRNA, 0.326A, 0.31U, 0.228G, 0.144C). Ribosomal RNA was also isolated from Drosophila melanogaster. Optical properties of these A + U-rich species were measured. In all four cases analysis of the hypochromic effect revealed that adenine and uracil residues tended to form clusters along the polynucleotide chain. A substantial fraction of residues was located in bihelical regions of approx. 50% G-C base pairs or in regions of approx. 30-35% G-C base pairs. The possible evolutionary significance of these results was considered on the basis of comparison with properties of rRNA from bacteria (Escherichia coli) and a mammal (rabbit reticulocyte). ...
To maintain the genomic integrity, cell has evolved various DNA repair pathways. Base Excision Repair pathway (BER) is one such DNA repair pathway which is dedicated to protect DNA from small lesions such as oxidation ...
Thymine is one of the five bases that form nucleic acids, along with adenine, guanine, cytosine, and uracil. The formula of thymine is C5H6N2O2. Thymine is always paired up with adenine through two hydrogen bonds only in DNA to stabilize the nucleic acid structure. Thymine is not present in RNA. Instead, uracil takes place of thymine and binds with adenine. It is a derivative of pyrimidine and can be derived by methylation of uracil at the 5th carbon, hence the other name of thymine, 5-methyluracil. Uracil takes its place in RNA, which also binds to adenine. Thymine is a single ring planar molecule. Thymine combined with deoxyribose yields deoxythymidine while Thymine with ribose makes thymidine.. Thymine binds with deoxyribose to form the nucleoside deoxythymidine, which is the same thing as thymidine. This compound can be phosphorylated with one, two, or three phosphoric acid groups creating thymidine mono-, di-, or triphosphate, respectively.. ...
Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated o-phenylenediamine directly tethered to n
Our ROTI®Kits for EdU mediated detection of cell proliferation provide a superior alternative to time-consuming and less sensitive bromodeoxyuridine (BrdU) assays. Similar to BrdU assays, our assays utilise a uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities and to the assay situation (e.g. for double or triple detection). Furthermore, the click reaction is highly selective and therefore prevents any unspecific labelling. The protocol includes only few steps and decreases the total amount of time.. ...
Our ROTI®Kits for EdU mediated detection of cell proliferation provide a superior alternative to time-consuming and less sensitive bromodeoxyuridine (BrdU) assays. Similar to BrdU assays, our assays utilise a uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities and to the assay situation (e.g. for double or triple detection). Furthermore, the click reaction is highly selective and therefore prevents any unspecific labelling. The protocol includes only few steps and decreases the total amount of time.. ...
A second extensive search used all identified key words and index terms. Some general considerations on the excited-state dynamics tadalafila como funciona of uracil derivatives are also reported. The findings of meta-analyses regarding surgical versus non-surgical treatment for acute Achilles tendon ...
The Stivers Lab is broadly interested in the biology of the RNA base uracil when it is present in DNA. Our work involves structural and biophysical studies of uracil recognition by DNA repair enzymes, the central role of uracil in adapative and innate immunity, and the function of uracil in antifolate and fluoropyrimidine chemotherapy. We use a wide breadth of structural, chemical, genetic and biophysical approaches that provide a fundamental understanding of molecular function. Our long-range goal is to use this understanding to design novel small molecules that alter biological pathways within a cellular environment. One approach we are developing is the high-throughput synthesis and screening of small molecule libraries directed at important targets in cancer and HIV-1 pathogenesis.. Research Areas: biophysics, enzymes, cell biology, uracil, cancer, HIV, DNA, RNA ...
Deuteration at the nitrogen sites of uracil produces a striking change in the dissociative attachment cross-section at electron energies below 3 eV. In particular, sharp structures observed in uracil are absent or too small to observe. This result, as well as theoretical modeling, supports the earlier assignment of the sharp structure to vibrational Feshbach resonances that decay by tunneling of the N1 hydrogen atom through the barrier created by the avoided crossing of the dipole bound anion potential surface by that of the lowest 2Σ valence anion state.
Uracil permease, UraA. The crystal structure of UraA with bound uracil at 2.8 Å resolution is available (PDB: 3QE7) (Lu et al., 2011). UraA has a novel structural fold, with 14 TMSs divided into two inverted repeats. A pair of antiparallel β-strands is located between TMS3 and TMS10 and has an important role in structural organization and substrate recognition. The structure is spatially arranged into a core domain and a gate domain. Uracil, located at the interface between the two domains, is coordinated mainly by residues from the core domain. Structural analysis suggests that alternating access of the substrate may be achieved through conformational changes of the gate domain. Multiscale molecular dynamics simulations of the UraA symporter in phospholipid bilayers revealed a closed state with 3 high affinity binding sites for cardolipin (Kalli et al. 2015).The crystal structure of UraA bound to uracil in an occluded state at 2.5 A resolution (Yu et al. 2017). UraA shows substantial motions ...
Uracil is a base found in RNA. It pairs with adenine and replaces thymine in DNA. Uracil, a pyrimidine, was originally discovered in 1900. ...
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The recommended starting alogliptin dose is 25 mg once a day. This portion of the eMedTV Web library outlines some factors that will determine your dosage of alogliptin and offers tips on how to most effectively take the medication.
When dUTP replaced dTTP during polyoma DNA replication in isolated cell nuclei, radioactivity from labeled deoxynucleoside triphosphates was almost exclusively recovered in very short Okazaki fragments and incorporation ceased after a short time. Addition of uracil, a known inhibitor of the enzyme u …
It is easily picked up because uracil is not a natural base in DNA. It is found normally in RNA, and it is quite possible that RNA evolved first in the earliest life on earth, and when DNA took over the role it has today of storing genetic information thymine was substituted in place of uracil so that the deamination process could be detected and repaired. In other words, if uracil was a natural base in DNA, there would be no way for the proof-reading machinery to detect that a base change occurred. Pretty ingenious of nature, dont you think ...
AID (activation-induced cytidine deaminase ) er et mutator-protein som deaminerer cytosin til uracil i immunoglobulin (Ig)-genene i B-celler. AID er essensielt i vårt adaptive immunforsvar, mens feilregulert AID-aktivitet er relatert til mutasjoner og kreft. Vi har studert aktivitet, intracellulær transport og regulering av AID. For å bekjempe nye infeksjoner produserer stimulerte B celler antistoffer med økt affinitet og endrede beskyttelsesfunksjoner. Dette skjer ved at mutasjoner introduseres med høy frekvens i Ig-genene, og AID er proteinet som initierer disse prosessene ved å deaminere cytosin til uracil. Ved feil regulering av AID vil uracil som er generert i proto-oncogener være en tidlig og kritisk hendelse ved utvikling av B-celle lymfom. Ekspresjon, enzymatisk aktivitet og intracellulær lokalisering må derfor være nøye regulert for å unngå uønskede mutasjoner. AID er i hovedsak lokalisert til cytoplasma, og for at AID skal deaminere cytosin til uracil i Ig-genene må det ...
In the early 1960s, Marshall Nirenberg and National Institutes of Health colleagues focused on how DNA directs protein synthesis and the role of RNA in these processes. Their 1961 experiment, using a synthetic messenger RNA (mRNA) strand that contained only uracils (U), yielded a protein that contained only phenylalanines. Identifying UUU (three uracil bases in a row) as the
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I while back (in the distant days when i was in education...Im a day out of education now!!) we were studying amino acids in chemistry, and I asked my chemistry teacher whether uracil was an amino acid or not. She didnt know but said she would find out, which she didnt, so I am asking the experts now ...
We studied the physiological response to limitation by diverse nutrients in batch and steady-state (chemostat) cultures of S. cerevisiae. We found that the global pattern of transcription in steady-state cultures in limiting phosphate or sulfate is essentially identical to that of batch cultures growing in the same medium just before the limiting nutrient is completely exhausted. The massive stress response and complete arrest of the cell cycle that occurs when nutrients are fully exhausted in batch cultures is not observed in the chemostat, indicating that the cells in the chemostat are poor, not starving. Similar comparisons using leucine or uracil auxotrophs limited on leucine or uracil again showed patterns of gene expression in steady-state closely resembling those of corresponding batch cultures just before they exhaust the nutrient. Although there is also a strong stress response in the auxotrophic batch cultures, cell cycle arrest, if it occurs at all, is much less uniform. Many of the ...
1JLR: The structural mechanism of GTP stabilized oligomerization and catalytic activation of the Toxoplasma gondii uracil phosphoribosyltransferase.
Each nucleotide in RNA contains a ribose, whose carbons are numbered 1 through 5. The base - often adenine, cytosine, guanine or uracil - is attached to the 1 position. A phosphate group is attached to the 3 position of one ribose and the 5 position of the next. The phosphate groups have a negative charge each at physiological pH, making RNA a charged molecule. The bases often form hydrogen bonds between adenine and uracil and between cytosine and guanine, but other interactions are possible,[1] such as a group of adenine bases binding to each other in a bulge.[2] There are also numerous modified bases and sugars found in RNA that serve many different roles. Pseudouridine (Ψ), in which the linkage between uracil and ribose is changed from a C-N bond to a C-C bond, and ribothymidine (T), are found in various places (most notably in the TΨC loop of tRNA).[3] Another notable modified base is hypoxanthine, a deaminated guanine base whose nucleoside is called inosine. Inosine plays a key role ...
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CJ236 Electrocompetent Cells High efficiency cells to create uracil-containing DNA for site-directed mutagenesis Sole source of highly efficient Electrocompetent cells (1 × 109 cfu/µg) ung- and dut- mutations to generate DNA... ...
CJ236 Electrocompetent Cells High efficiency cells to create uracil-containing DNA for site-directed mutagenesis Sole source of highly efficient Electrocompetent cells (1 × 109 cfu/µg) ung- and dut- mutations to generate DNA... ...
Uracil in RNA replaces thymine in DNA, according to ScienceDaily. Both uracil and thymine bond with adenine, the complementary base found in both the RNA and DNA...
Biomolecules - Nucleic acids and proteins (Part B) from GTAC. Nucleic acids (DNA and RNA) are the next group of macromolecules that we are looking at. Nucleic acids are made up of monomer units that consist of a phosphate group, a sugar unit and a nitrogenous base. The nitrogenous bases in DNA are Thymine (T), Adenine (A), Guanine(G) and Cytosine (C). In RNA, the Thymine (T) is replaced by Uracil (U). Thymine (or Uracil in RNA) always pairs with Adenine (2 hydrogen bonds) and Guanine always pairs with Cytosine (3 hydrogen bonds).. ...
Immunohistochemical staining of mirror sections in this study showed expression of both Arp2 and WAVE2 in the same adenocarcinoma of the lung cells, and the clinical data indicated that coexpression of Arp2 and WAVE2 was an independent risk factor for tumor recurrence. The state of coexpression of Arp2 and WAVE2 would determine whether strong adjuvant chemotherapy should be done especially at stage IA; coexpression was also shown to affect the overall survival rate of the 115 patients and was significantly correlated with lymph node metastasis. These results suggest that coexpression of Arp2 and WAVE2 is involved in a mechanism that augments the malignant potential of the tumor cells.. To analyze the relationship between coexpression and mild chemotherapy, which is mainly composed of oral uracil and tegafur in this study, we divided 77 cases of stage I into two cases with positive coexpression and with negative coexpression. In 45 positive coexpression cases, patients who received chemotherapy ...
Genomic DNA undergoes a chemical treatment. The procedure converts all non-methylated cytosines into a different base, uracil (uracils hybridization behaviour is identical to that of thymine), using the chemical bisulphite ...
So many thanks to Mary and Rosella for your suggestions. I shall be sure to write a few more poems now based on your ideas. A little teaser- the fourth poem is tenetatively titled Adenine and Uracil- A Molecular Beanstalk/ Jack and The Giant- A Tale of DNA Replication/ Fee Fi Fo Fum- The Role of DNA ...
Krokan, Hans Einar; Kavli, Bodil Merete; Akbari, Mansour; Visnes, Torkild; Pettersen, Henrik P Sahlin; Sundheim, Ottar; Hagen, Lars; Otterlei, Marit; Gilljam, Karin Margaretha; Slupphaug, Geir. (2007) Repair og uracil in DNA by UNG2 and SMUG1 - an update. DNA Repair. ...
There are many ways to show the genetic code, the map between triplets of nucleotides and the amino acids of proteins. Here is one that may be a bit awkward to understand, but other more standard ones are easily found. First, here are the codes for the four nucleotides: U = Uracil C = Cytosine…
* found in: NADP Disodium Salt Trihydrate, DNTP Set, 2-Deoxyguanosine Monohydrate, Adenosine-5-Triphosphate (ATP), Uracil, DATP 100 mm Solution pH 7.0,..
Hello! The contents of this entry were transferred to MomsterTeachers new home! Find the contents here: UPDATE: Brain Trains UPCAT Passers and Outstanding DLSU CAT, ACET, and UPCAT Passers
... has role mouse metabolite (CHEBI:75771) uracil (CHEBI:17568) has role prodrug (CHEBI:50266) uracil (CHEBI: ... uracil-5-carboxylic acid (CHEBI:17477) has functional parent uracil (CHEBI:17568). uracil-6-ylacetic acid (CHEBI:46371) has ... uracil (CHEBI:17568). 1-(ethoxymethyl)-5-isopropyl-6-(phenylsulfanyl)uracil (CHEBI:40152) has functional parent uracil (CHEBI: ... uracil (CHEBI:17568) has role Daphnia magna metabolite (CHEBI:83056) uracil (CHEBI:17568) has role Escherichia coli metabolite ...
In DNA, the uracil nucleobase is replaced by thymine. Uracil is a demethylated form of thymine. Uracil is a common and ... In yeast, uracil concentrations are inversely proportional to uracil permease. Mixtures containing uracil are also commonly ... Uracil is a weak acid. The first site of ionization of uracil is not known. The negative charge is placed on the oxygen anion ... When uracil reacts with anhydrous hydrazine, a first-order kinetic reaction occurs and the uracil ring opens up. If the pH of ...
Uracil dehydrogenase (EC 1.1.99.19, uracil oxidase) is an enzyme with systematic name uracil:(acceptor) oxidoreductase. This ... Uracil+dehydrogenase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Hayaishi O, Kornberg A (May 1952). "Metabolism of cytosine, thymine, uracil, and barbituric acid by bacterial enzymes". The ... enzyme catalyses the following chemical reaction uracil + acceptor ⇌ {\displaystyle \rightleftharpoons } barbiturate + reduced ...
Ab initio Methods: We performed ab initio calculations on a number of variants of cytosine and uracil structures which we ... Uracil as an Alternative. to 5-Fluorocytosine in Addressable Protein Targeting by John A. Wendel and Steven S. Smith ... The initial nucleophilic attack of uracil by the enzyme is not favored because the resulting intemediate must develop negative ...
... uracil; Uracil, di(trimethylsilyl) deriv.; Uracil, bis-TMS; Uracil, diTMS; Uracil bis(trimethylsilyl) ether; Uracil, TMS; ... Uracil, 2TMS derivative. *Formula: C10H20N2O2Si2 ...
... is a aminouracil (CHEBI:22532) 5-amino-6-(D-ribitylamino)uracil (CHEBI:15934) is ... 5-amino-6-(D-ribitylamino)uracil (CHEBI:15934) has functional parent ribitol (CHEBI:15963) 5-amino-6-(D-ribitylamino)uracil ( ... CHEBI:15934 - 5-amino-6-(D-ribitylamino)uracil. Main. ChEBI Ontology. Automatic Xrefs. Reactions. Pathways. Models. ... 5-amino-6-(D-ribitylamino)uracil (CHEBI:15934) has role Saccharomyces cerevisiae metabolite (CHEBI:75772) 5-amino-6-(D- ...
Learn about the potential side effects of Uracil (doxycycline). Includes common and rare side effects information for consumers ... Some of the dosage forms listed on this page may not apply to the brand name Uracil. ... Along with its needed effects, doxycycline (the active ingredient contained in Uracil) may cause some unwanted effects. ...
Efficient site-directed mutagenesis using uracil-containing DNA.. Kunkel TA, Bebenek K, McClary J. ...
Ahn J, Vu T, Novince Z, Guerrero-Santoro J, Rapic-Otrin V, Gronenborn AM (2010) HIV-1 Vpr loads uracil DNA glycosylase-2 onto ... Krokan HE, Drabløs F, Slupphaug G (2002) Uracil in DNA: occurrence, consequences and repair. Oncogene 21:8935-8948CrossRef ... Fenard D, Houzet L, Bernard E, Tupin A, Brun S, Mougel M, Devaux C, Chazal N, Briant L (2009) Uracil DNA glycosylase 2 ... Mansky LM, Preveral S, Selig L, Benarous R, Benichou S (2000) The interaction of vpr with uracil DNA glycosylase modulates the ...
Crystal structures of Toxoplasma gondii uracil phosphoribosyltransferase reveal the atomic basis of pyrimidine discrimination ...
Uracil je jedna od četiri nukleobaza RNK, koja zamenjuje timin koji se nalazi u DNK. Kao i timin, uracil može da formira bazni ... Uracil će, za razliku od timina, lakše da se degeneriše u citozin. Uracil se vrlo retko može naći u DNK. ... uracil: Dihidrouracil • 3-Ureidopropionska kiselina • β-Alanin. timin: Dihidrotimin • β-Ureidoizobuterna kiselina • 3- ... Dobavljeno iz "https://sh.wikipedia.org/w/index.php?title=Uracil&oldid=33653562" ...
Uracil is a base found in RNA. It pairs with adenine and replaces thymine in DNA. Uracil, a pyrimidine, was originally ... Retrieved from "https://simple.wikipedia.org/w/index.php?title=Uracil&oldid=6465669" ...
A Moderate Drug Interaction exists between coccidioidin skin test and uracil mustard. View detailed information regarding this ... coccidioidin skin test uracil mustard. Applies to: coccidioidin skin test and uracil mustard ... Depending on the dose and length of time you have been on uracil mustard, you may have a reduced response to the skin test. It ... If you are currently being treated or have recently been treated with uracil mustard, you should let your doctor know before ...
Base excision repair initiation revealed by crystal structures and binding kinetics of human uracil-DNA glycosylase with DNA. ... Uracil-DNA glycosylase-like Uracil-DNA glycosylase-like Uracil-DNA glycosylase Uracil-DNA glycosylase Human (Homo sapiens) [ ...
A. Pałasz and D. Cież, "In search of uracil derivatives as bioactive agents. Uracils and fused uracils: synthesis, biological ... Molecular Modeling Studies of Some Uracil and New Deoxyuridine Derivatives. Yousra Abdel-Mottaleb1 and M. S. A. Abdel-Mottaleb2 ... A. Shah, E. Nosheen, F. Zafar et al., "Photochemistry and electrochemistry of anticancer uracils," Journal of Photochemistry ... T. Lukmanov, P. Sergey, M. Edward, and L. Sergey, "Relative stability of keto-enol tautomers in 5,6-substituted uracils: ab ...
Uracil , C4H4N2O2 , CID 1174 - structure, chemical names, physical and chemical properties, classification, patents, literature ...
... and uracil in a molar ratio of 1:4. It was developed as an anti-cancer therapy by Taiho Pharmaceutical Co Ltd.It is approved in ... Tegafur-uracil is an anti-tumor compound containing tegafur (1-(2-tetrahydrofuryl)-5-fluorouracil) ... Tegafur-uracil is an anti-tumor compound containing tegafur (1-(2-tetrahydrofuryl)-5-fluorouracil) and uracil in a molar ratio ... Tegafur-uracil. Clinical Trials. Clinical Trials Phase. Status. Purpose. Conditions. Count. 1. Completed. Treatment. Colorectal ...
1. Prepare Uracil-containing DNA (e.g. PCR amplification product). The Uracil Cleavage System enzymes are active in most ... The Uracil Cleavage System provides two enzymes, which, when added sequentially to a reaction containing a synthetic DNA ... 1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of uracil in 30 minutes from double-stranded, ... UDG catalyses the excision of the uracil base, creating an abasic site with an intact phosphodiester backbone (1,2). The lyase ...
with the uracil O23. The ultimate reaction catalyzed by UDG cleavage of the glycosidic bond between the uracil base and its ... hydrogen bonds to the uracil N3. The closest interaction between the enzyme and the uracil involves two with the Asn204 of the ... Uracil-DNA Glycosylase. Rei Mitsuyama 15 and Holden Richards 15 Contents:. I. Introduction II. General Structure III. DNA ... Uracil-DNA glycosylase is lesion-specific (as the name suggests) for in DNA. For DNA to be bound by UDG, it must be in the B ...
Modifying Uracil DNA Glycosylase within Biological Sciences at the University of Southampton ... Uracil-DNA glycosylase (UDGase) selectively recognises and cleaves uracil residues in DNA (which are produced by the mutagenic ... Research project: Modifying Uracil DNA Glycosylase. Currently Active: Yes. We are generating mutants of this DNA repair enzyme ...
Uracil, 6-methyl-4-propyl- , C8H12N2O2 , CID 165610 - structure, chemical names, physical and chemical properties, ...
This patient-friendly article is about chemotherapy drug, Tegafur with uracil (Uftoral) which is a chemotherapy drug most often ... It is a combination of two drugs - tegafur and uracil and is what is known as an anti-metabolite which stops cells making and ... The uracil interacts with the tegafur and stops 5FU breaking down. This means that there is more of the active drug in your ...
... coli uracil N-glycosylase gene, which has been inserted into an E. coli host to direct the expression of the wild type form of ... AmpErase Uracil N-Glycosylase (UNG), part of the GeneAmp PCR Carry-over Prevention Kit, is a 26 kDa ultrapure, recombinant ... Uracil N-glycosylase (UNG) prior to PCR amplification. Products from PCR amplification contain uracil, and are readily ... The enzyme removes any uracil incorporated into single- or double-stranded DNA.. GeneAmp® PCR Carry-over Prevention Kit. The ...
a) A comparison of the ability of DNA polymerases to amplify DNA fragments using uracil-free (-dU) and uracil-containing ... Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.. Nour-Eldin HH1, Hansen BG, ... The largely unused uracil-excision molecular cloning technique has excellent features in most aspects compared to other modern ... A PCR fragment amplified with compatible uracil-containing primers by the PfuTurbo® Cx Hotstart DNA polymerase is mixed with ...
... *Download PDF Copy ... Surprisingly, they found that the uracil-rich RNA fragment in the mutated-type SARS-CoV-2 viruses increased the production of ... China had a disproportionate number of cytosine bases that were switched to uracil, in addition to a number of other nucleotide ...
Addition of uracil, a known inhibitor of the enzyme u … ... Addition of uracil, a known inhibitor of the enzyme uracil-DNA ... Formation of Okazaki fragments in polyoma DNA synthesis caused by misincorporation of uracil Cell. 1978 Mar;13(3):573-80. doi: ... This effect was reversed completely by uracil. The short strands formed from dUTP could be chased efficiently into long strands ... contain an excision-repair system which by removal of uracil causes strand breakage and under certain circumstances may ...
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due ... Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due ... Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.UniRule annotation. Manual assertion according to rulesi ... Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.UniRule annotation ...
Uracil, allosteric regulator and coenzyme (CAS 66-22-8), with ,98% purity. Water soluble compound. Join researchers using our ...
... catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or ... Uracil DNA Glycosylase, UNG.. Description. E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil ... Uracil DNA Glycosilase although stable at 15°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles. ... UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases). ...

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