A cell line derived from cultured tumor cells.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.
The total amount (cell number, weight, size or volume) of tumor cells or tissue in the body.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.
A transplantable, poorly differentiated malignant tumor which appeared originally as a spontaneous breast carcinoma in a mouse. It grows in both solid and ascitic forms.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
Experimentally induced mammary neoplasms in animals to provide a model for studying human BREAST NEOPLASMS.
A malignant kidney tumor, caused by the uncontrolled multiplication of renal stem (blastemal), stromal (STROMAL CELLS), and epithelial (EPITHELIAL CELLS) elements. However, not all three are present in every case. Several genes or chromosomal areas have been associated with Wilms tumor which is usually found in childhood as a firm lump in a child's side or ABDOMEN.
Genes that inhibit expression of the tumorigenic phenotype. They are normally involved in holding cellular growth in check. When tumor suppressor genes are inactivated or lost, a barrier to normal proliferation is removed and unregulated growth is possible.
Neoplasms of the intracranial components of the central nervous system, including the cerebral hemispheres, basal ganglia, hypothalamus, thalamus, brain stem, and cerebellum. Brain neoplasms are subdivided into primary (originating from brain tissue) and secondary (i.e., metastatic) forms. Primary neoplasms are subdivided into benign and malignant forms. In general, brain tumors may also be classified by age of onset, histologic type, or presenting location in the brain.
Experimental transplantation of neoplasms in laboratory animals for research purposes.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Exfoliate neoplastic cells circulating in the blood and associated with metastasizing tumors.
The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A usually small, slow-growing neoplasm composed of islands of rounded, oxyphilic, or spindle-shaped cells of medium size, with moderately small vesicular nuclei, and covered by intact mucosa with a yellow cut surface. The tumor can occur anywhere in the gastrointestinal tract (and in the lungs and other sites); approximately 90% arise in the appendix. It is now established that these tumors are of neuroendocrine origin and derive from a primitive stem cell. (From Stedman, 25th ed & Holland et al., Cancer Medicine, 3d ed, p1182)
Transplantation between animals of different species.
A malignant epithelial tumor with a glandular organization.
A sarcoma derived from deep fibrous tissue, characterized by bundles of immature proliferating fibroblasts with variable collagen formation, which tends to invade locally and metastasize by the bloodstream. (Stedman, 25th ed)
In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.
Tumors whose cells possess secretory granules and originate from the neuroectoderm, i.e., the cells of the ectoblast or epiblast that program the neuroendocrine system. Common properties across most neuroendocrine tumors include ectopic hormone production (often via APUD CELLS), the presence of tumor-associated antigens, and isozyme composition.
Tumors or cancer of the COLON.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Tumors or cancer of the human BREAST.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Experimentally induced neoplasms of CONNECTIVE TISSUE in animals to provide a model for studying human SARCOMA.
Experimentally induced tumor that produces MELANIN in animals to provide a model for studying human MELANOMA.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Established cell cultures that have the potential to propagate indefinitely.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
DNA present in neoplastic tissue.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS.
Antibodies produced by a single clone of cells.
Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Tumors or cancer of the LIVER.
Tumors or cancers of the KIDNEY.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Tumors or cancer of the SKIN.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Tumors or cancer of the LUNG.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
Elements of limited time intervals, contributing to particular results or situations.
Tumors or cancer of the MAMMARY GLAND in animals (MAMMARY GLANDS, ANIMAL).
All tumors in the GASTROINTESTINAL TRACT arising from mesenchymal cells (MESODERM) except those of smooth muscle cells (LEIOMYOMA) or Schwann cells (SCHWANNOMA).
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.
Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.
Methods which attempt to express in replicable terms the extent of the neoplasm in the patient.
The worsening of a disease over time. This concept is most often used for chronic and incurable diseases where the stage of the disease is an important determinant of therapy and prognosis.
Vaccines or candidate vaccines designed to prevent or treat cancer. Vaccines are produced using the patient's own whole tumor cells as the source of antigens, or using tumor-specific antigens, often recombinantly produced.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Tumors or cancer located in bone tissue or specific BONES.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The relationship between the dose of an administered drug and the response of the organism to the drug.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
RNA present in neoplastic tissue.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Gonadal interstitial or stromal cell neoplasm composed of only LEYDIG CELLS. These tumors may produce one or more of the steroid hormones such as ANDROGENS; ESTROGENS; and CORTICOSTEROIDS. Clinical symptoms include testicular swelling, GYNECOMASTIA, sexual precocity in children, or virilization (VIRILISM) in females.
Processes required for CELL ENLARGEMENT and CELL PROLIFERATION.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Adherence of cells to surfaces or to other cells.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
A general term for various neoplastic diseases of the lymphoid tissue.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Experimentally induced tumors of the LIVER.
A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Transfer of a neoplasm from its primary site to lymph nodes or to distant parts of the body by way of the lymphatic system.
A rare but highly lethal childhood tumor found almost exclusively in infants. Histopathologically, it resembles RHABDOMYOSARCOMA but the tumor cells are not of myogenic origin. Although it arises primarily in the kidney, it may be found in other parts of the body. The rhabdoid cytomorphology is believed to be the expression of a very primitive malignant cell. (From Holland et al., Cancer Medicine, 3d ed, p2210)
Ability of neoplasms to infiltrate and actively destroy surrounding tissue.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Highly proliferative, self-renewing, and colony-forming stem cells which give rise to NEOPLASMS.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51)
A transplantable carcinoma of the rat that originally appeared spontaneously in the mammary gland of a pregnant albino rat, and which now resembles a carcinoma in young transplants and a sarcoma in older transplants. (Stedman, 25th ed)
A neoplasm composed entirely of GRANULOSA CELLS, occurring mostly in the OVARY. In the adult form, it may contain some THECA CELLS. This tumor often produces ESTRADIOL and INHIBIN. The excess estrogen exposure can lead to other malignancies in women and PRECOCIOUS PUBERTY in girls. In rare cases, granulosa cell tumors have been identified in the TESTES.
The treatment of a disease or condition by several different means simultaneously or sequentially. Chemoimmunotherapy, RADIOIMMUNOTHERAPY, chemoradiotherapy, cryochemotherapy, and SALVAGE THERAPY are seen most frequently, but their combinations with each other and surgery are also used.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Lymphocytes that show specificity for autologous tumor cells. Ex vivo isolation and culturing of TIL with interleukin-2, followed by reinfusion into the patient, is one form of adoptive immunotherapy of cancer.
The local recurrence of a neoplasm following treatment. It arises from microscopic cells of the original neoplasm that have escaped therapeutic intervention and later become clinically visible at the original site.
Tumors or cancer of the URINARY BLADDER.
A condition of decreased oxygen content at the cellular level.
Neoplasms of the brain and spinal cord derived from glial cells which vary from histologically benign forms to highly anaplastic and malignant tumors. Fibrillary astrocytomas are the most common type and may be classified in order of increasing malignancy (grades I through IV). In the first two decades of life, astrocytomas tend to originate in the cerebellar hemispheres; in adults, they most frequently arise in the cerebrum and frequently undergo malignant transformation. (From Devita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2013-7; Holland et al., Cancer Medicine, 3d ed, p1082)
Antineoplastic antibiotic obtained from Streptomyces peucetius. It is a hydroxy derivative of DAUNORUBICIN.
Agents and endogenous substances that antagonize or inhibit the development of new blood vessels.
A carcinoma discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass. (From Cancer Chemother Rep 2 1972 Nov;(3)1:325) It is also called 3LL and LLC and is used as a transplantable malignancy.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Tumor suppressor genes located on the short arm of human chromosome 17 and coding for the phosphoprotein p53.
The action of a drug in promoting or enhancing the effectiveness of another drug.
A unifocal malignant tumor that consists of atypical pathological MAST CELLS without systemic involvement. It causes local destructive growth in organs other than in skin or bone marrow.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A heterogeneous group of sporadic or hereditary carcinoma derived from cells of the KIDNEYS. There are several subtypes including the clear cells, the papillary, the chromophobe, the collecting duct, the spindle cells (sarcomatoid), or mixed cell-type carcinoma.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Drugs used to potentiate the effectiveness of radiation therapy in destroying unwanted cells.
Neoplasms which arise from or metastasize to the PITUITARY GLAND. The majority of pituitary neoplasms are adenomas, which are divided into non-secreting and secreting forms. Hormone producing forms are further classified by the type of hormone they secrete. Pituitary adenomas may also be characterized by their staining properties (see ADENOMA, BASOPHIL; ADENOMA, ACIDOPHIL; and ADENOMA, CHROMOPHOBE). Pituitary tumors may compress adjacent structures, including the HYPOTHALAMUS, several CRANIAL NERVES, and the OPTIC CHIASM. Chiasmal compression may result in bitemporal HEMIANOPSIA.
A sarcoma originating in bone-forming cells, affecting the ends of long bones. It is the most common and most malignant of sarcomas of the bones, and occurs chiefly among 10- to 25-year-old youths. (From Stedman, 25th ed)
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
A class of statistical procedures for estimating the survival function (function of time, starting with a population 100% well at a given time and providing the percentage of the population still well at later times). The survival analysis is then used for making inferences about the effects of treatments, prognostic factors, exposures, and other covariates on the function.
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Soft tissue tumors or cancer arising from the mucosal surfaces of the LIP; oral cavity; PHARYNX; LARYNX; and cervical esophagus. Other sites included are the NOSE and PARANASAL SINUSES; SALIVARY GLANDS; THYROID GLAND and PARATHYROID GLANDS; and MELANOMA and non-melanoma skin cancers of the head and neck. (from Holland et al., Cancer Medicine, 4th ed, p1651)
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Tumors or cancer of the STOMACH.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Proteins prepared by recombinant DNA technology.
Tumors or cancer of the GASTROINTESTINAL TRACT, from the MOUTH to the ANAL CANAL.
A benign epithelial tumor with a glandular organization.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS.
Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals.
The ability of some cells or tissues to survive lethal doses of IONIZING RADIATION. Tolerance depends on the species, cell type, and physical and chemical variables, including RADIATION-PROTECTIVE AGENTS and RADIATION-SENSITIZING AGENTS.
Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.
A tumor necrosis factor receptor subtype that has specificity for TUMOR NECROSIS FACTOR ALPHA and LYMPHOTOXIN ALPHA. It is constitutively expressed in most tissues and is a key mediator of tumor necrosis factor signaling in the vast majority of cells. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
The proportion of survivors in a group, e.g., of patients, studied and followed over a period, or the proportion of persons in a specified group alive at the beginning of a time interval who survive to the end of the interval. It is often studied using life table methods.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A connective tissue neoplasm formed by proliferation of mesodermal cells; it is usually highly malignant.
Chemical substances, produced by microorganisms, inhibiting or preventing the proliferation of neoplasms.
A group of malignant tumors of the nervous system that feature primitive cells with elements of neuronal and/or glial differentiation. Use of this term is limited by some authors to central nervous system tumors and others include neoplasms of similar origin which arise extracranially (i.e., NEUROECTODERMAL TUMORS, PRIMITIVE, PERIPHERAL). This term is also occasionally used as a synonym for MEDULLOBLASTOMA. In general, these tumors arise in the first decade of life and tend to be highly malignant. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, p2059)
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Tomography using x-ray transmission and a computer algorithm to reconstruct the image.
An inorganic and water-soluble platinum complex. After undergoing hydrolysis, it reacts with DNA to produce both intra and interstrand crosslinks. These crosslinks appear to impair replication and transcription of DNA. The cytotoxicity of cisplatin correlates with cellular arrest in the G2 phase of the cell cycle.
Tumors or cancer of the TESTIS. Germ cell tumors (GERMINOMA) of the testis constitute 95% of all testicular neoplasms.
Human COLORECTAL CARCINOMA cell line.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A type of connective tissue neoplasm typically arising from intralobular stroma of the breast. It is characterized by the rapid enlargement of an asymmetric firm mobile mass. Histologically, its leaf-like stromal clefts are lined by EPITHELIAL CELLS. Rare phyllodes tumor of the prostate is also known.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Form of adoptive transfer where cells with antitumor activity are transferred to the tumor-bearing host in order to mediate tumor regression. The lymphoid cells commonly used are lymphokine-activated killer (LAK) cells and tumor-infiltrating lymphocytes (TIL). This is usually considered a form of passive immunotherapy. (From DeVita, et al., Cancer, 1993, pp.305-7, 314)
A relatively common neoplasm of the CENTRAL NERVOUS SYSTEM that arises from arachnoidal cells. The majority are well differentiated vascular tumors which grow slowly and have a low potential to be invasive, although malignant subtypes occur. Meningiomas have a predilection to arise from the parasagittal region, cerebral convexity, sphenoidal ridge, olfactory groove, and SPINAL CANAL. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2056-7)
The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
A blue-red, extremely painful vascular neoplasm involving a glomeriform arteriovenous anastomosis (glomus body), which may be found anywhere in the skin, most often in the distal portion of the fingers and toes, especially beneath the nail. It is composed of specialized pericytes (sometimes termed glomus cells), usually in single encapsulated nodular masses which may be several millimeters in diameter (From Stedman, 27th ed). CHEMODECTOMA, a tumor of NEURAL CREST origin, is also sometimes called a glomus tumor.
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9)
Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
A class of fibrous proteins or scleroproteins that represents the principal constituent of EPIDERMIS; HAIR; NAILS; horny tissues, and the organic matrix of tooth ENAMEL. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms a coiled-coil alpha helical structure consisting of TYPE I KERATIN and a TYPE II KERATIN, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. alpha-Keratins have been classified into at least 20 subtypes. In addition multiple isoforms of subtypes have been found which may be due to GENE DUPLICATION.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A glycoprotein that is secreted into the luminal surface of the epithelia in the gastrointestinal tract. It is found in the feces and pancreaticobiliary secretions and is used to monitor the response to colon cancer treatment.
Tumors of bone tissue or synovial or other soft tissue characterized by the presence of giant cells. The most common are giant cell tumor of tendon sheath and GIANT CELL TUMOR OF BONE.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
Compounds that are used in medicine as sources of radiation for radiotherapy and for diagnostic purposes. They have numerous uses in research and industry. (Martindale, The Extra Pharmacopoeia, 30th ed, p1161)
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Non-invasive method of demonstrating internal anatomy based on the principle that atomic nuclei in a strong magnetic field absorb pulses of radiofrequency energy and emit them as radiowaves which can be reconstructed into computerized images. The concept includes proton spin tomographic techniques.
Injections introduced directly into localized lesions.
A bone tumor composed of cellular spindle-cell stroma containing scattered multinucleated giant cells resembling osteoclasts. The tumors range from benign to frankly malignant lesions. The tumor occurs most frequently in an end of a long tubular bone in young adults. (From Dorland, 27th ed; Stedman, 25th ed)
The loss of one allele at a specific locus, caused by a deletion mutation; or loss of a chromosome from a chromosome pair, resulting in abnormal HEMIZYGOSITY. It is detected when heterozygous markers for a locus appear monomorphic because one of the ALLELES was deleted.
A malignant neoplasm that may be classified either as a glioma or as a primitive neuroectodermal tumor of childhood (see NEUROECTODERMAL TUMOR, PRIMITIVE). The tumor occurs most frequently in the first decade of life with the most typical location being the cerebellar vermis. Histologic features include a high degree of cellularity, frequent mitotic figures, and a tendency for the cells to organize into sheets or form rosettes. Medulloblastoma have a high propensity to spread throughout the craniospinal intradural axis. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2060-1)
The pathological process occurring in cells that are dying from irreparable injuries. It is caused by the progressive, uncontrolled action of degradative ENZYMES, leading to MITOCHONDRIAL SWELLING, nuclear flocculation, and cell lysis. It is distinct it from APOPTOSIS, which is a normal, regulated cellular process.
Malignant neoplasms arising in the neuroectoderm, the portion of the ectoderm of the early embryo that gives rise to the central and peripheral nervous systems, including some glial cells.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.
A large group of proteins that control APOPTOSIS. This family of proteins includes many ONCOGENE PROTEINS as well as a wide variety of classes of INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS such as CASPASES.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Spherical, heterogeneous aggregates of proliferating, quiescent, and necrotic cells in culture that retain three-dimensional architecture and tissue-specific functions. The ability to form spheroids is a characteristic trait of CULTURED TUMOR CELLS derived from solid TUMORS. Cells from normal tissues can also form spheroids. They represent an in-vitro model for studies of the biology of both normal and malignant cells. (From Bjerkvig, Spheroid Culture in Cancer Research, 1992, p4)
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.

Transformation mediated by RhoA requires activity of ROCK kinases. (1/52805)

BACKGROUND: The Ras-related GTPase RhoA controls signalling processes required for cytoskeletal reorganisation, transcriptional regulation, and transformation. The ability of RhoA mutants to transform cells correlates not with transcription but with their ability to bind ROCK-I, an effector kinase involved in cytoskeletal reorganisation. We used a recently developed specific ROCK inhibitor, Y-27632, and ROCK truncation mutants to investigate the role of ROCK kinases in transcriptional activation and transformation. RESULTS: In NIH3T3 cells, Y-27632 did not prevent the activation of serum response factor, transcription of c-fos or cell cycle re-entry following serum stimulation. Repeated treatment of NIH3T3 cells with Y-27632, however, substantially disrupted their actin fibre network but did not affect their growth rate. Y-27632 blocked focus formation by RhoA and its guanine-nucleotide exchange factors Dbl and mNET1. It did not affect the growth rate of cells transformed by Dbl and mNET1, but restored normal growth control at confluence and prevented their growth in soft agar. Y-27632 also significantly inhibited focus formation by Ras, but had no effect on the establishment or maintenance of transformation by Src. Furthermore, it significantly inhibited anchorage-independent growth of two out of four colorectal tumour cell lines. Consistent with these data, a truncated ROCK derivative exhibited weak ability to cooperate with activated Raf in focus formation assays. CONCLUSIONS: ROCK signalling is required for both the establishment and maintenance of transformation by constitutive activation of RhoA, and contributes to the Ras-transformed phenotype. These observations provide a potential explanation for the requirement for Rho in Ras-mediated transformation. Moreover, the inhibition of ROCK kinases may be of therapeutic use.  (+info)

Caspase-mediated cleavage of p21Waf1/Cip1 converts cancer cells from growth arrest to undergoing apoptosis. (2/52805)

The cyclin-dependent kinase inhibitor p21waf1/Cip1 is a downstream effector of the p53-dependent cell growth arrest. We report herein that p21 was cleaved by caspase-3/CPP32 at the site of DHVD112L during the DNA damage-induced apoptosis of cancer cells. The cleaved p21 fragment could no more arrest the cells in G1 phase nor suppress the cells undergoing apoptosis because it failed to bind to the proliferating cell nuclear antigen (PCNA) and lost its capability to localize in the nucleus. Thus, caspase-3-mediated cleavage and inactivation of p21 protein may convert cancer cells from growth arrest to undergoing apoptosis, leading to the acceleration of chemotherapy-induced apoptotic process in cancer cells.  (+info)

Decreased expression of the pro-apoptotic protein Par-4 in renal cell carcinoma. (3/52805)

Par-4 is a widely expressed leucine zipper protein that confers sensitization to apoptosis induced by exogenous insults. Because the expression of genes that promote apoptosis may be down-regulated during tumorigenesis, we sought to examine the expression of Par-4 in human tumors. We present here evidence that Par-4 protein levels were severely decreased in human renal cell carcinoma specimens relative to normal tubular cells. Replenishment of Par-4 protein levels in renal cell carcinoma cell lines conferred sensitivity to apoptosis. Because apoptosis may serve as a defense mechanism against malignant transformation or progression, decreased expression of Par-4 may contribute to the pathophysiology of renal cell carcinoma.  (+info)

Activation of Src in human breast tumor cell lines: elevated levels of phosphotyrosine phosphatase activity that preferentially recognizes the Src carboxy terminal negative regulatory tyrosine 530. (4/52805)

Elevated levels of Src kinase activity have been reported in a number of human cancers, including colon and breast cancer. We have analysed four human breast tumor cell lines that exhibit high levels of Src kinase activity, and have determined that these cell lines also exhibit a high level of a phosphotyrosine phosphatase activity that recognizes the Src carboxy-terminal P-Tyr530 negative regulatory site. Total Src kinase activity in these cell lines is elevated as much as 30-fold over activity in normal control cells and specific activity is elevated as much as 5.6-fold. When the breast tumor cells were grown in the presence of the tyrosine phosphatase inhibitor vanadate, Src kinase activity was reduced in all four breast tumor cell lines, suggesting that Src was being activated by a phosphatase which could recognize the Tyr530 negative regulatory site. In fractionated cell extracts from the breast tumor cells, we found elevated levels of a membrane associated tyrosine phosphatase activity that preferentially dephosphorylated a Src family carboxy-terminal phosphopeptide containing the regulatory tyrosine 530 site. Src was hypophosphorylated in vivo at tyrosine 530 in at least two of the tumor cell lines, further suggesting that Src was being activated by a phosphatase in these cells. In preliminary immunoprecipitation and antibody depletion experiments, we were unable to correlate the major portion of this phosphatase activity with several known phosphatases.  (+info)

Caspase 3 inactivation to suppress Fas-mediated apoptosis: identification of binding domain with p21 and ILP and inactivation machinery by p21. (5/52805)

The death mediator caspase acts as the dominant regulator during cell death induction. The CPP32 subfamily, including caspase 3 (CPP32/Yama/Apopain), is essential for the cell death signaling. We recently reported that activation of caspase 3 is regulated by complex formation with p21 or ILP. In the present study, we investigated the binding domain with p21 and ILP to further characterize the caspase 3 inactivation machinery. Our results show that caspase 3 contains p21 binding domain in the N-terminus and ILP binding domain in the active site. Further, the caspase 3 binding domain in p21 was independent of the Cdk- or PCNA-binding domain. We also found caspase 3 protection by p21 from the p3-site cleavage serineproteinase contributes to the suppression machinery. Here, we propose the caspase 3 inactivation system by p21 and ILP as new essential system in the regulation of cell death.  (+info)

Phenotypic analysis of human glioma cells expressing the MMAC1 tumor suppressor phosphatase. (6/52805)

MMAC1, also known as PTEN or TEP-1, was recently identified as a gene commonly mutated in a variety of human neoplasias. Sequence analysis revealed that MMAC1 harbored sequences similar to those found in several protein phosphatases. Subsequent studies demonstrated that MMAC1 possessed in vitro enzymatic activity similar to that exhibited by dual specificity phosphatases. To characterize the potential cellular functions of MMAC1, we expressed wild-type and several mutant variants of MMAC1 in the human glioma cell line, U373, that lacks endogenous expression. While expression of wild-type MMAC1 in these cells significantly reduced their growth rate and saturation density, expression of enzymatically inactive MMAC1 significantly enhanced growth in soft agar. Our observations indicate that while wild-type MMAC1 exhibits activities compatible with its proposed role as a tumor suppressor, cellular expression of MMAC1 containing mutations in the catalytic domain may yield protein products that enhance transformation characteristics.  (+info)

Detailed methylation analysis of the glutathione S-transferase pi (GSTP1) gene in prostate cancer. (7/52805)

Glutathione-S-Transferases (GSTs) comprise a family of isoenzymes that provide protection to mammalian cells against electrophilic metabolites of carcinogens and reactive oxygen species. Previous studies have shown that the CpG-rich promoter region of the pi-class gene GSTP1 is methylated at single restriction sites in the majority of prostate cancers. In order to understand the nature of abnormal methylation of the GSTP1 gene in prostate cancer we undertook a detailed analysis of methylation at 131 CpG sites spanning the promoter and body of the gene. Our results show that DNA methylation is not confined to specific CpG sites in the promoter region of the GSTP1 gene but is extensive throughout the CpG island in prostate cancer cells. Furthermore we found that both alleles are abnormally methylated in this region. In normal prostate tissue, the entire CpG island was unmethylated, but extensive methylation was found outside the island in the body of the gene. Loss of GSTP1 expression correlated with DNA methylation of the CpG island in both prostate cancer cell lines and cancer tissues whereas methylation outside the CpG island in normal prostate tissue appeared to have no effect on gene expression.  (+info)

Growth inhibition of breast cancer cells by Grb2 downregulation is correlated with inactivation of mitogen-activated protein kinase in EGFR, but not in ErbB2, cells. (8/52805)

Increased breast cancer growth has been associated with increased expression of epidermal growth factor receptor (EGFR) and ErbB2 receptor tyrosine kinases (RTKs). Upon activation, RTKs may transmit their oncogenic signals by binding to the growth factor receptor bound protein-2 (Grb2), which in turn binds to SOS and activates the Ras/Raf/MEK/mitogen-activated protein (MAP) kinase pathway. Grb2 is important for the transformation of fibroblasts by EGFR and ErbB2; however, whether Grb2 is also important for the proliferation of breast cancer cells expressing these RTKs is unclear. We have used liposomes to deliver nuclease-resistant antisense oligodeoxynucleotides (oligos) specific for the GRB2 mRNA to breast cancer cells. Grb2 protein downregulation could inhibit breast cancer cell growth; the degree of growth inhibition was dependent upon the activation and/or endogenous levels of the RTKs. Grb2 inhibition led to MAP kinase inactivation in EGFR, but not in ErbB2, breast cancer cells, suggesting that different pathways might be used by EGFR and ErbB2 to regulate breast cancer growth.  (+info)

Recombinant human sHer2 produced by HEK 293 and sHer2 isolated from the supernatant of the primary tumor cell culture from breast cancer patients was used in the experiments. The oligomeric status was estimated using the method of gel-filtration chromatography and electrophoresis. The stability of Her2 was determined by the change in the protein secondary structure under variation of temperature and pH values by means of CD spectroscopy. The inhibitory activity of sHer2 was analyzed in tests with Her2-expressing cell cultures. The content of sHer2 in the supernatant of primary tumor cell cultures from breast cancer patients was determined using the sandwich ELISA. Amplification of the HER2 gene in the primary tumor cells was verified employing the FISH. ...
We investigated the antiproliferative effects of extracellular nicotinamide adenine dinucleotide against human malignant CaCo-2 (colon carcinoma), Hep
Zhang M, Lykke-Andersen S, Zhu B, et al. Characterising-regulatory variation in the transcriptome of histologically normal and tumour-derived pancreatic tissues. Gut. 2018;67(3):521-533. doi:10.1136/gutjnl-2016-313146. ...
Zhang M, Lykke-Andersen S, Zhu B, et al. Characterising-regulatory variation in the transcriptome of histologically normal and tumour-derived pancreatic tissues. Gut. 2018;67(3):521-533. doi:10.1136/gutjnl-2016-313146. ...
TY - JOUR. T1 - Characterization of a newly established human pancreatic carcinoma cell line, UK Pan-1. AU - Fralix, Kimberly D.. AU - Ahmed, Mansoor M.. AU - Mattingly, Cynthia. AU - Swiderski, Carol. AU - McGrath, Patrick C.. AU - Venkatasubbarao, Kolaparthi. AU - Kamada, Nanao. AU - Mohiuddin, Mohammed. AU - Strodel, William E.. AU - Freeman, James W.. PY - 2000/5/1. Y1 - 2000/5/1. N2 - BACKGROUND. A highly tumorigenic cell line designated as UK Pan-1 was established in a surgically removed human pancreatic adenocarcinoma and characterized as having many of the genotypic and phenotypic alterations commonly found in pancreatic tumors. METHODS. The cell line was characterized by its morphology, growth rate in monolayer culture and soft agar, tumorigenicity in nude mice, and chromosomal analysis. Furthermore, the status of p53, Ki-ras mutation and transforming growth factor (TGF)-β receptor expression were determined. The characteristics of UK Pan-1 were compared with those of other commonly ...
Cytokine production by the human bladder carcinoma cell line T24 in the presence of bacillus Calmette-Guerin (BCG).: The study was initiated as an in vitro appr
Substantial multiplication in vitro of cloned cells from a human embryonal carcinoma cell line, Tera 2, has been obtained in a basal medium (DMEM/Hams F12,50:50, v/v) supplemented with 10 micrograms low density lipoprotein/ml, 100 micrograms high density lipoprotein/ml, 100 ng multiplication stimulating activity/ml, 100 ng insulin/ml and 1 microgram transferrin/ml. The growth rate appears to be similar to that obtained in 10% serum. Furthermore, studies on the expression of cell surface receptors revealed that cloned Tera 2 cells express high-affinity receptors for IGF-II but not for insulin. The cells also express receptors for Epidermal Growth Factor (EGF) even though the addition of EGF does not stimulate their proliferation in serum-free medium. These results suggest that the expression of specific growth factor receptors is not an absolute determinant of hormone responsiveness. ...
Apoptosis is a form of cell death in which the cell actively participates. Apoptosis was induced in two human leukaemic cell lines, U937 and HL-60, by incubation with a diverse array of chemical agents. Cell death was assessed by gel electrophoresis, light microscopy and flow cytometry. It was demonstrated that apoptosis involved the formation of large kilobase pair DNA fragments (20-50, 145-245 and 580 kilobase pairs) prior to, or accompanying, internucleosomal cleavage. Degradation of DNA to large kilobase pair sizes also occurred in some forms of necrosis. These fragments were similar, but not identical, to those generated during apoptosis. The identity of the endonuclease(s) responsible for DNA cleavage to large kilobase pair fragments is as yet unknown. One suggestion is that topoisomerase II might be involved. Using an HL-60 subclone with reduced topoisomerase II expression, it was shown that topoisomerase II was not necessary for the formation of large kilobase pair DNA fragments and ...
TY - JOUR. T1 - Expression of the matrix metalloproteinase promatrilysin in coculture of prostate carcinoma cell lines. AU - Stratton, M. S.. AU - Sirvent, H.. AU - Udayakumar, T. S.. AU - Nagle, R. B.. AU - Bowden, G. T.. PY - 2001/8/22. Y1 - 2001/8/22. N2 - BACKGROUND. Matrix metalloproteinases (MMPs) are involved in tumor progression. Matrilysin (MMP-7) has been shown to be upregulated in prostatic carcinomas and can increase the invasive capacity of DU-145 cells. Because of the heterogenous nature of prostatic tumors, we examined promatrilysin expression in cocultures containing two different prostatic carcinoma cell lines, DU-145 and LNCaP. METHODS. Using enzyme linked immunosorbent assay (ELISA) analyses, promatrilysin expression was measured in DU-145/LNCaP cocultures and conditioned media cross-cultures. The effects of blocking IL-6 on promatrilysin expression were examined by pretreating conditioned media with IL-6 neutralizing antibody. RESULTS. A significant induction of promatrilysin ...
BioAssay record AID 103718 submitted by ChEMBL: In vitro cytotoxicity against human breast carcinoma cell line MCF-7 at 8.1*10e-5M.
In-Vitro Cytotoxic Effect of Linderina madayiparense Extracts against Human Lung Carcinoma Cell Lines (A-549), Umakrithika Chiranjeevi, Anandarajagopal Kalusalingam, Ajaykumar Than
A cell line has been derived from a human prostatic carcinoma xenograft, CWR22R. This represents one of very few available cell lines representative of this disease. The cell line is derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the …
TY - JOUR. T1 - Protective immunity induced by B7.1+IL-12 transfected tumor cells is abrogated by tumor growth in an immune privileged site. AU - Chen, P. W.. AU - Uno, T.. AU - Geer, D. G.. AU - Podack, E. R.. AU - Ksander, B. R.. PY - 1996/12/1. Y1 - 1996/12/1. N2 - Mice immunized with B7.1+IL-12 transfected tumor cells possess systemic anti-tumor immunity that protects mice from a second subcutaneous tumor challenge of untransfected cells. This study determines if these immunized mice are protected from a second tumor challenge delivered to an immunologically privileged site. Our previous results indicated that tumors growing within the immunologically privileged anterior chamber of the eye (AC) of naive mice induce systemic T cell tolerance. In these experiments we determined if tolerance could be imposed after mice were previously sensitized. DBA/2 mice were given a subcutaneous immunization with 1 × 106 P815 B7.1+IL-12 cells. One month later, mice received a second tumor challenge of ...
TY - JOUR. T1 - Suppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-β receptor type II. AU - Zhao, Wenli. AU - Kobayashi, Masonobu. AU - Ding, Wei. AU - Yuan, Lan. AU - Seth, Prem. AU - Cornain, Santoso. AU - Wang, Jingxin. AU - Okada, Futoshi. AU - Hosokawa, Masuo. PY - 2002/9/20. Y1 - 2002/9/20. N2 - We have previously reported that transforming growth factor beta (TGF-β) produced by rat hepatoma cell line KDH-8 cells suppressed the interleukin-2 (IL-2) production of T cells and the tumoricidal activity of macrophages in KDH-8 tumor-bearing rats and that the inhibition of TGF-β production by low-dose bleomycin restored these activities significantly. In this study, we established three transfectant clones with stable expression of soluble TGF-β receptor type II (sTRII), namely KT1, KT2 and KT3, and one with an empty vector used as control vector (KV), and then investigated the effects of sTRII on the tumorigenicity of KDH-8 cells and immune responses ...
Many versions of the free Acrobat Reader do not allow Save. You must instead save the PDF from the CG&D Online page you downloaded it from. PC users: Right-click on the Download link and choose the option that says something like Save Link As.... Mac users should hold the mouse button down on the link to get these same options. ...
Prostaglandins are synthesized from arachidonic acid by the enzyme cyclo-oxygenase. There are two isoforms of cyclooxygenases: COX-1 (a constitutive form) and COX-2 (an inducible form). COX-2 has recently been categorized as an immediate-early gene and is associated with cellular growth and differen …
Polyamines (PAs) are involved in regulation of cell growth and cellular survival by interacting with processes like translation, transcription or ion transport. It is described that polyamines can induce apoptosis in mesenchymal cell lines. The aim of our study was to analyze whether the physiological PAs (putrescine, spermidine or spermine) or the PA-derivate deoxyspergualin (DSG), a novel immunosuppressant, induce apoptosis in immunocompetent cells. Furthermore, we wanted to investigate which molecular mechanisms are involved in the execution of the cell death program. By means of flow cytometric analysis we found an induction of apoptosis by spermine (Spm) and DSG in quiescent and activated PBMCs, PHA generated lymphoblasts, and various tumor cell lines (Jurkat, SKW-3, U937). Moreover, DSG and Spm triggered apoptosis in human Fas-deficient cells and in cell lines MV4.11. and RS4.11., which are described to be resistant to apoptosis induction by many conventional chemotherapeutic agents. ...
BioAssay record AID 104079 submitted by ChEMBL: In vitro cytotoxicity against human mammary carcinoma cells 180 fold resistant to adriamycin MCF7-R.
1 Growth of Human Lung Tumor Cells in Culture Reen Wu Center for Comparative Respiratory Biology and Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California,
Tumour cells release membrane micro(nano)fragments called tumour-derived microvesicles (TMV) that are believed to play an important role in cancer progression. TMV suppress/modify antitumour response of the host, but there is also some evidence for their direct interaction with cancer cells. In cancer patients TMV are
3020 A variety of human carcinoma cells express high levels of interleukin-13 receptorα2 chain, a primary binding subunit of IL-13R complex in vitro and in vivo. These receptors could be targeted by a chimeric fusion protein consisting of Interleukin-13 and a truncated form of Pseudomonas exotoxin (IL-13PE38). Microarray analysis of adrenomedullin (AM) transfected human prostate tumor PC-3 cells showed that human and rat AM up-regulated the IL-13Rα2 chain gene. We now demonstrate that IL-13Rα2 chain is also up regulated in two human breast tumor cell lines (MCF-7 and MDA-MB-231) and one prostate carcinoma (PC-3) cell line after treatment with AM. RT-PCR results confirmed that mRNAs for IL-13Rα2 were enhanced 2.5 to 4 fold in 24 and 48 hr AM treated cells, respectively compared to control cells. Indirect immunofluorescence assay (IFA) revealed that protein level of IL-13Rα2 were also increased in AM treated breast and prostate carcinoma cells. To understand the mechanism of up-regulation, ...
Choong et al. used a database of expressed proteins for a hepatocellular carcinoma-derived cell line (HCC-M), separated by two-dimensional gel electrophoresis, that they had developed previously. The separated proteins were fingerprinted by using peptide mass spectrometric techniques. From these data they could identify novel proteins that were not present in the SWISS-PROT or National Center for Biotechnology Infromation (NCBI) databases. One such protein was then taken for further analysis and was subjected to in-gel trypsin digestion and the products were sequenced using mass spectrometry. The sequences were then used to search an expressed sequence tag (EST) database and 40 DNA sequences obtained were assembled into a putative open reading frame. On the basis of this consensus DNA sequence, the authors named the protein HCC-1, and confirmed and extended the sequence using RACE (rapid amplification of cDNA ends).. A battery of bioinformatics tools was used to analyze the predicted protein, ...
TGFβ signaling has typically been associated with suppression of tumor initiation while the role it plays in metastasis is generally associated with progression of malignancy. However, we present evidence here for an anti-metastatic role of TGFβ signaling. To test the importance of TGFβ signaling to cell survival and metastasis we compared human colon carcinoma cell lines that are either non-tumorigenic with TGFβ response (FET), or tumorigenic with TGFβ response (FETα) or tumorigenic with abrogated TGFβ response via introduction of dominant negative TGFβRII (FETα/DN) and their ability to metastasize. Metastatic competency was assessed by orthotopic transplantation. Metastatic colony formation was assessed histologically and by imaging. Abrogation of TGFβ signaling through introduction of a dominant negative TGFβ receptor II (TGFβRII) in non-metastatic FETα human colon cancer cells permits metastasis to distal organs, but importantly does not reduce invasive behavior at the primary site.
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Buy our SK N SH (human neuroblastoma cell line) whole cell lysate. ab3956 has been validated in western blot. Abcam now offers a 12-month guarantee.
By analysing a human lung tumour cell line subtraction cDNA library, we have identified and characterized a novel member of the human S100 gene family that we designated S100A14. The full-length cDNA is 1067 bp and encodes a putative protein of 104 amino acids. The predicted protein contains the S100-specific EF-hand calcium-binding domain. The gene is ubiquitously expressed in normal human tissues of epithelial origin. S100A14 transcript was found to be down-regulated in many immortalized and tumour cell lines from diverse tissues. In contrast to the tumour cell lines, S100A14 shows up-regulation at the mRNA and protein level in many human primary tumours, including lung and breast carcinomas. To elucidate mechanisms whereby S100A14 expression is enhanced in lung and breast tumours, we studied the effects of epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) on its expression. Both are ligands of ERBB receptor and induced S100A14 expression in the immortalized ...
Cellular Biology:, Neoplasm:, Serology: Antigen, Tissue Culture:, Types of Tumors:, Transplantable Tumors: YCAB, Genes: H-2 - Histocompatibility-2, Strains: BALB/C. ...
IL-13Rα2 chain has been shown to play a unique role in tumor biology. It is overexpressed in a variety of primary tumor cell cultures and tumor cell lines (13, 15-18, 25, 34), whereas normal cells including lymphoid cells, endothelial cells, and astrocytes (13, 15, 17, 26) do not express or express low levels of this cytokine receptor chain. Recent studies have demonstrated that overexpression of IL-13Rα2 chain in certain breast and pancreatic cancer cell lines resulted into loss of tumorigenicity, whereas unmodified control tumor cells formed enlarging tumor nodules when injected in immunodeficient mice (46). In another study, Terabe et al. (47) have demonstrated that the soluble form of IL-13Rα2 chain (5) can modulate immune environment and shift Th2 phenotype to a dominant Th1 phenotype. This shift resulted into resistance of 15-12RM cell-derived tumor recurrence. Thus, further investigation of the role of IL-13Rα2 chain in tumor immunology and targeting has become extremely ...
Work in this thesis was primarily involved with the characterisation of four receptors, the turkey and bovine P2Y1, and the human P2Y2 and P2Y4 receptors, transfected into the human astrocytoma cell line, 1321N1. It also describes the preliminary characterisation of two vascular smooth muscle cell types, and the generation of P2Y1-GST fusion proteins for the production of antibodies.;1321N1 cells were found to release nucleotides in response to sheer stress, but this was overcome by adaptation of the stimulation method. 1321N1 cells expressing the four receptors were assayed for PLC activity, and the rank order of agonist potency for each receptor was generally consistent with reports in the literature. Nucleotide interconversion at the surface of 1321N1 cells was also examined indirectly using the ecto-ATPase inhibitor ARL 67156 and hexokinase. Although there was no significant effect of nucleotide breakdown on the agonist concentration-responses, it was found that upstream conversion of ...
Many cancer patients develop tumor-reactive immune responses against antigens that are either expressed on the surface of tumor cells or released from them into the peripheral circulation. In this study, tumor-reactive immunoglobulins, present in the sera of ovarian cancer patients, were used to identify commonly recognized tumor-associated antigens on ovarian tumor cells. Western immunoblot analysis of cellular proteins, obtained from UL-1 ovarian tumor cell line, demonstrated several commonly recognized immunoreactive proteins. Two of these proteins (Mr 32,000 and 71,000) were selected for further investigation. Cellular proteins isolated from normal human ovarian epithelia, in a similar fashion, failed to exhibit corresponding immunoreactivity to these proteins. As an additional control, sera from normal (nontumor-bearing) individuals failed to identify these proteins on Western immunoblots. Furthermore, the absorption of the ovarian cancer patients sera with normal ovarian epithelial tissue ...
Cell culture. Six gastric cancer cell lines (KE-39, KE-97, HuG1-N, HuG1-PI, ECC-10, and ECC-12) were incubated in RPMI 1640 with 10% FCS (Life Technologies/Invitrogen Corp.) at 37°C. Other tumor cell lines were maintained in DMEM with 10% FCS (Life Technologies/Invitrogen Corp.) at 37°C. FK228 (a gift from Fujisawa Corporation) was used at concentrations of 1.0 to 2.5 ng/mL.. Plasmids and transfection. pCAGF1-IG (empty vector), pCAGF1-IG-Brm, pCAGF1-IG-Brm-KR, pCAGF1-IG-BRG1, and pCAGF1-IG-BRG1-KR were prepared as described previously ( 26). All transfections were done using Lipofectamine Plus (Invitrogen Corp.).. Western blotting. An anti-Brm antibody (Transgenic, Inc.), anti-BRG1 antibody (H-88; Santa Cruz Biotechnology), and anti-BAF47/Ini1 antibody (BD Transduction Laboratories) were used, and immunostaining was done as described previously ( 24).. Immunohistochemistry. Cells were fixed with 4% paraformaldehyde at 37°C for 1 h. After treatments with 0.2% Triton-X100 for 20 min at room ...
A stable LifeAct-TagGFP2 expressing human fibrosarcoma cell line Long-term actin visualization - 25 passages guaranteed Characteristics proven to be identica...
Aberrant growth factor production is a prevalent mechanism in tumourigenesis. If T-cells responded positively to a cancer-derived cytokine, this might result in selective enhancement of function within the tumour microenvironment. Here, we have chosen colony-stimulating factor-1 (CSF-1) as a candidate to test this concept. CSF-1 is greatly overproduced in many cancers but has no direct effects upon T-lymphocytes, which do not express the c-fms-encoded CSF-1 receptor. To confer CSF-1-responsiveness, we have expressed the human c fins gene in immortalized and primary T-cells. Addition of soluble CSF-1 resulted in synergistic enhancement of IL-2-driven T-cell proliferation. CSF-1 also co-stimulated the production of interferon (IFN)-gamma by activated T-cells. These effects required Y809 of the CSF-1R and activation of the Ras-MEK-MAP kinase cascade, but were independent of PI3K signalling. T-cells that express c-fins are also responsive to membrane-anchored CSF-1 (mCSF-1) which, unlike its soluble ...
Biolidics and the detection of tumoral cells on bloodstream , the new machine for liquid biopsies that allows to detects tumoral cells.
IL-4(38-37)-PE38KDEL is a targeted fusion protein that specifically attaches to cells expressing IL-4Rs reported to be present on many different solid tumor cells of varying pathology (21) . After binding to the IL-4R, the fusion protein is internalized, and then the toxin portion of the molecule causes the ADP ribosylation of elongation factor 2 and arrests protein synthesis (22) . The cell dies by apoptosis attributable to the lack of new protein synthesis (23) . Human brain tumor cells are particularly enriched in the expression of IL-4Rs on their cell surface (9 , 10) . Although numerous preclinical safety and efficacy experiments have been performed with IL-4(38-37)-PE38KDEL, this targeted drug has not been administered to humans before. Here we demonstrate for the first time that intraglioma administration of IL-4(38-37)-PE38KDEL to patients is safe. No treatmentrelated deaths or life-threatening toxicities were observed in any patient. In six of nine patients, administration of ...
Great progress has been made over the past two decades in the development of animal models for ovarian cancer. Each generation of animal model has had its advantages and limitations. The earliest models used a xenograft approach in which human ovarian tumor cells or tissues were grown in immunodeficient mice (17, 27, 32, 33, 36). The xenograft model preserved the complex interactions that occur between cancer cells and their microenvironment, including stromal-epithelial cell interactions, as well as influences of matrix proteins, growth factors, and angiogenesis. Thus, this model was a great advance over cell culture model systems and advanced the study of therapeutic interventions. However, an important weakness in the xenograft model was the lack of host immunity, which severely limited the ability to reliably predict the effect of noncancer immune-host influences on outcomes. In addition, tumors were introduced in the xenograft model rather than arising as primary lesions in the ovary, thus ...
The purpose of this study was to evaluate the effect of plasma-activated media (PAM) on a multicellular tumor spheroid (MCTS) obtained using HCT116 colon carcin...
This unit describes the use of retroviral vectors that can be successfully employed for gene transfer into both primary tumor cultures and established cell lines
CLS weist eine Sammlung von insgesamt 30 verschiedenen Tumorzellen des Gehirns auf (Stand: Dezember 2016). Eine bersicht ber alle derzeitigen Zelllinien isoliert aus Hirntumoren k nnen Sie unter Panel Brain Tumor cell lines. als PDF herunterladen. Die Informationen sind unterteilt in Tabelle 1, die allgemeine Daten enth lt, sowie in Tabelle 2, die Zellmarker, Tumorantigene, Mutationen sowie Zytokine, soweit bekannt, auflistet. ...
CLS weist eine Sammlung von insgesamt 30 verschiedenen Tumorzellen des Gehirns auf (Stand: Dezember 2016). Eine bersicht ber alle derzeitigen Zelllinien isoliert aus Hirntumoren k nnen Sie unter Panel Brain Tumor cell lines. als PDF herunterladen. Die Informationen sind unterteilt in Tabelle 1, die allgemeine Daten enth lt, sowie in Tabelle 2, die Zellmarker, Tumorantigene, Mutationen sowie Zytokine, soweit bekannt, auflistet. ...
Tumor-derived cell lines matched to either normal or metastatic cell lines obtained from the same patient provide a valuable resource for cancer studies. The availability of such models allows researchers to compare tumor lines to their normal counterparts.
Tumor-derived cell lines matched to either normal or metastatic cell lines obtained from the same patient provide a valuable resource for cancer studies. The availability of such models allows researchers to compare tumor lines to their normal counterparts.
Pathology Department, Harvard Medical School, Boston, Massachusetts 02115-5701, USA. We have investigated the functional integrity of the retinoblastoma tumor suppressor pathway in five human squamous cell carcinoma lines. Elevated activity of cyclin-dependent kinase 6 (cdk6), a pRB kinase, was detected in all five squamous cell carcinoma lines. Overexpression of the cdk6 protein was detected in one of the five cell lines. The cdk6-specific inhibitor p18ink4C is expressed and associated with cdk6 in all five squamous cell carcinoma lines. In contrast, only very low levels of p16ink4A were detected in these cell lines. This may contribute to the elevated activity of cdk6 in these lines. Elevated activity of cdk6 may result in hyperphosphorylation of the retinoblastoma protein and, therefore, compromise its negative growth-regulatory activity ...
TY - JOUR. T1 - Cantharidin-induced cytotoxicity and cyclooxygenase 2 expression in human bladder carcinoma cell line. AU - Huan, Steven Kuan Hua. AU - Lee, Hao Hsien. AU - Liu, Der Zen. AU - Wu, Chien Chih. AU - Wang, Ching Chiung. PY - 2006/6/1. Y1 - 2006/6/1. N2 - Mylabris is used in clinical therapy, but is always accompanied by cystitis. The toxic effects of mylabris on bladder are attributed to its active principle: cantharidin. In the present study, we explored how cantharidin induces cytotoxicity in the bladder. Human bladder carcinoma cell line T 24 cells were used as target cells, and human colon carcinoma HT 29 cells as native cells. Cantharidin exhibited acute cytotoxicity in the T 24 cells, and IC50 was 21.8, 11.2 and 4.6 μM after treatment for 6, 24 and 48 h, respectively. The cytotoxicity of cantharidin was not significantly enhanced when T 24 cells were treated for a longer time. Moreover, PARP proteins and pro-caspase 3, Bcl-2 were significantly inhibited after cantharidin ...
Despite multimodal treatment malignant gliomas still remain incurable. Molecular markers represent a useful tool for the development of tailored therapeutic options. In gliomas the transcription factor HIF-1α, a key mediator of hypoxic response, is a poor prognostic marker. In contrast, mutations in the IDH enzymes are associated with better outcome for patients with glioma. The aim of the present thesis was to investigate the influence of HIF-1α-Inhibition and expression of mutant IDH1 on cell biological and radiobiological behavior of malignant glioma cell lines. Targeting HIF-1α attenuated the hypoxia-induced radioresistance of the investigated glioma cell lines. Furthermore, overexpression of mutant IDH1 caused a less aggressive phenotype and was directly linked to an increased sensitivity to radiotherapy of glioma cells independent of the oxygen conditions. In conclusion, inhibition of HIF-1α is a promising strategy to sensitize hypoxic gliomas to radiotherapy and detection of the ...
To investigate the patterns of genetic lesions in a panel of 23 human multiple myeloma cell lines (HMCLs), we made a genomic integrative analysis involving FISH, and both gene expression and genome-wide profiling approaches. The expression profiles of the genes targeted by the main IGH translocations showed that the WHSC1/MMSET gene involved in t(4;14)(p16;q32) was expressed at different levels in all of the HMCLs, and that the expression of the MAF gene was not restricted to the HMCLs carrying t(14;16)(q32;q23). Supervised analyses identified a limited number of genes specifically associated with t(4;14) and involved in different biological processes. The signature related to MAF/MAFB expression included the known MAF target genes CCND2 and ITGB7, as well as genes controlling cell shape and cell adhesion. Genome-wide DNA profiling allowed the identification of a gain on chromosome arm 1q in 88% of the analyzed cell lines, together with recurrent gains on 8q, 18q, 7q, and 20q; the most frequent ...
It was reported previously that the specific aim of screening agents that would induce apoptosis in the human breast carcinoma cell line MDA-MB-231 was completed, and that calyculin A was the most effective compound tested. Since then Cdc25 phosphatases were reported as possible key oncogenes in human breast carcinoma. In light of our results with calyculin A, it was decided that a more promising focus for the project would be the biochemical basis for the oncogenic actions of Cdc25 phosphatases in human breast carcinoma. At this time we have examined 18 compounds and discovered a Cdc25 phosphatase inhibitor, called 1f, that selectively inhibits Cdc25A,B, and C, has antiproliferative activity against and causes a G1 block in MDA-MB-231 cells. The pure form of this compound and two other compounds which selectively inhibit Cdc25 phosphatases have been made and also demonstrate an equal if not better inhibition of Cdc25 phosphatases as their combinatorial form.*HUMANS
In the present study we describe the establishment and characteristics of a new human tumor cell line (OV-1063) positive for carcinoembryonic antigen (CEA) originating from ovarian metastatic tumor cells. Analysis of the cultured cells during their in vitro adaptation period revealed while the primary culture exhibited a low proportion of CEA-positive cells, this proportion increased with culture passages and eventually more than 90% of the cells in the established line were CEA-positive. Thus, during the period of adaptation to in vitro growth, a selection for CEA-positive cells took place but the amount of CEA secreted per each positive cell seemed to be constant. Several tumor-associated characteristics were found positive on the established OV-1063 cell line. The in vitro growing cell line exhibited an abnormal chromosome pattern with a near-trisomy karyotype for some chromosomes, colony formation in soft agar as well as positive staining with a monoclonal antibody B38.1. Culture supernatants of the
A team of researchers at the Sylvester Comprehensive Cancer Center at the University of Miami Miller School of Medicine has developed a technique for growing ovarian cancer cell lines that mimic the original tumor in long-term cultures. The technique offers hope for quickly advancing precision medicine applications for a variety of cancers by enabling clinicians to test therapies on cells from a patients own tumor in the laboratory, thus identifying the most targeted therapy for that patient. The results of the research, which was led by Tan A. Ince, M.D., Ph.D., associate professor of pathology, Scientific Director of Sylvesters Live Tumor Culture Core and Tissue Bank Core Facility, and Director of the Tumor Stem Cell Division at the Interdisciplinary Stem Cell Institute, have been published online in the journal Nature Communications in an article entitled Characterization of Twenty-Five Ovarian Tumour Cell Lines that Phenocopy Primary Tumours. The key to Inces research is his development ...
298168476 - EP 0869803 B1 20030416 - ALLOGENEIC PARACRINE CYTOKINE TUMOR VACCINES - [origin: WO9724132A1] The present invention provides a method of treating cancer comprising (a) obtaining a tumor cell line, (b) modifying the tumor cell line to render it capable of producing an increased level of a cytokine relative to the unmodified tumor cell line, and (c) administering the tumor cell line to a mammalian host having at least one tumor that is the same type of tumor as that from which the tumor cell line was obtained, wherein the tumor cell line is allogeneic and is not MHC-matched to the host. The present invention also provides a pancreatic tumor cell line, a method and medium for obtaining such a tumor cell line, and a composition comprised of cells of a purified pancreatic tumor cell line.[origin: WO9724132A1] The present invention provides a method of treating cancer comprising (a) obtaining a tumor cell line, (b) modifying the tumor cell line to render it capable of producing an increased level
Cell lines are invaluable tools for biomedical research. Cancer cell lines are the foundation of cancer biology and the quest for drug treatments. Adenocarcinoma is a cancer of the epithelium (including colon) and originates in glandular tissue, such as skin surface layer and glands.. Product. These human adenocarcinoma cell lines have been previously licensed to commercial parties for research into human adenocarcinoma treatments.. These cell lines are available through Public Health England ECACC. Please make your purchase through the links below.. Imperial Innovations requests that commercial/for profit parties contact them first to discuss the terms of supply prior to Innovations authorising the release of supplies from ECACC.. ...
MMP-2 production by tumor cells has been demonstrated to play a fundamental role in ECM degradation and tumor cell invasion (for review see 5 . The recent finding of reduced tumor progression in MMP-2-deficient mice (57) highlights the importance of this molecule. In this study, we wished to determine how MMP-2 gene expression is regulated in human astroglioma cells, as a strong correlation has been observed between astroglioma invasion and MMP-2 expression (17, 19, 20, 22, 23, 27). Our results indicate that two cytokines, TNF-α and IFN-γ, partially inhibit MMP-2 gene expression and can function together in an additive manner for near-complete inhibition of MMP-2 expression in human astroglioma cells. TNF-α/IFN-γ inhibition of MMP-2 expression was observed at several levels: on gelatinolytic activity as determined by zymography, on protein expression (both ProMMP-2 and activated MMP-2) as assessed by immunoblotting, on MMP-2 mRNA expression, and on MMP-2 promoter activity. Our results also ...
MDA-MB-435, a member of the NCI-DTP panel of 60 human tumor cell lines, has been used for decades as a model of metastatic human breast cancer. This cell line was derived at M.D. Anderson in 1976 from a pleural effusion from a 31-year old woman with a history of breast cancer (Cailleau R, Olive M, Cruciger QV. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro. 1978 Nov;14(11):911-5.; Brinkley BR, Beall PT, Wible LJ, Mace ML, Turner DS, Cailleau RM. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 1980 Sep;40(9):3118-29.) Further background information on this cell line may be found at the M.D. Anderson Breast Cancer Cell Line Database.. Recent advances in gene expression analysis allow the opportunity to more fully characterize tumor cell lines. Analysis of MDA-MB-435, in conjunction with the rest of the NCI60 panel, revealed that the pattern of gene expression for MDA-MB-435 more closely ...
Data presented above show that the human fibrosarcoma cell line HT1080 is able to inhibit the growth of experimental metastases. These results suggest that this inhibition is due to the antiangiogenic activity of circulating TSP-1 that is released by the tumor cells in vivo, circulates at effective levels, and makes the tumor-bearing animals unable to mount an angiogenic response. The data also demonstrate the in vivo efficacy of soluble TSP-1, showing it can be used as a drug to prevent the growth of experimental metastases.. HT1080 produced concomitant tumor resistance directly by secreting active TSP-1, unlike previously studied rodent tumors that create an antiangiogenic state when the tumor cells, or associated stromal elements (29), secrete enzymes or activators of enzymes (30) that generate inhibitors from other molecules. We saw no significant evidence for in vivo breakdown of tumor-derived TSP-1 to fragments smaller than the 140 kDa monomer, a molecule that is as effective an ...
4Discussion. This study addressed the question of which are the cellular processes that sensitive leukemic cells induced to achieve tolerance to vincristine. To this end, the B-ALL cell line CCRF-SB was gradually exposed until cell proliferation was observed in the presence of 6nM vincristine, and the corresponding proteomic profile was compared to that of cells grown in the absence of the chemotherapeutic drug.. Chemoresistance may be intrinsic or acquired.18 The ability to tolerate high concentrations of chemotherapeutics of an intrinsically resistant cancer cell is not developed as a result of an exposition to the drugs; instead it is the result of genetic abnormalities the cell carries before exposition.18 By contrast, acquired chemoresistance is developed after the cancer cell is exposed to the drug and may be the result of molecular evolution of resistant clones.19 Experimental settings to study acquired chemoresistance include the comparison of matched paired samples at diagnosis and ...
Expression of programmed cell death ligand 1 (PD-L1) is an important process by which tumor cells suppress antitumor immunity in the tumor microenvironment. Bone marrow (BM)-derived immune cells are an important component of the tumor microenvironment. However, the link between PD-L1 induction on tumor cells and communication with BM cells is unknown. This study demonstrates that BM cells have a direct effect in inducing PD-L1 expression on tumor cells, which contributes to the tumor cells drug resistance. This novel discovery was revealed using a co-incubation system with BM cells and tumor cells. BM cells from wild-type C57BL6 mice and the immune-deficient mouse strains B-cell−/−, CD28−/−, perforin−/−, and Rag2−/− but not CD11b−/− dramatically increased the expression of tumor cell surface PD-L1. This PD-L1 induction was dependent on CD11b-positive BM cells through direct contact with tumor cells. Furthermore, p38 signaling was activated in tumor cells after co-incubation with BM
Expression of programmed cell death ligand 1 (PD-L1) is an important process by which tumor cells suppress antitumor immunity in the tumor microenvironment. Bone marrow (BM)-derived immune cells are an important component of the tumor microenvironment. However, the link between PD-L1 induction on tumor cells and communication with BM cells is unknown. This study demonstrates that BM cells have a direct effect in inducing PD-L1 expression on tumor cells, which contributes to the tumor cells drug resistance. This novel discovery was revealed using a co-incubation system with BM cells and tumor cells. BM cells from wild-type C57BL6 mice and the immune-deficient mouse strains B-cell−/−, CD28−/−, perforin−/−, and Rag2−/− but not CD11b−/− dramatically increased the expression of tumor cell surface PD-L1. This PD-L1 induction was dependent on CD11b-positive BM cells through direct contact with tumor cells. Furthermore, p38 signaling was activated in tumor cells after co-incubation with BM
Natural products, especially supplementary metabolites produced by plants under stressed conditions, are shown to have different pharmacological impacts from one to another. cell lines. The existing study aims to research the power of crude nonpolar, semi-polar, and polar components of leaves to activate different required mechanisms that may prevent tumor cell proliferation or stimulate tumor cell apoptosis. 2. Outcomes: 2.1. Cytotoxicity The ready crude components had been examined against different tumor cell lines: MCF-7, HCT-116, and HepG2. The outcomes exposed that hexane and ethyl acetate components produced a substantial impact in comparison to in solid tumor cell lines MCF-7, HCT-116, and HepG2. Cells had been subjected to the components for 72 h. Cell viability was determined using SRB-U SulphoRhodamine-B data and assay are expressed as mean S.D. (n = 3). Desk 1 IC50 (g/mL) of different components of in various solid tumor cell lines. for 48 h, stained with AO/EB. The pictures had been ...
Pancreatic cancer has long carried poor prognosis. The development of new therapeutic approaches is particularly urgent. Inactivation of the tumor-suppressor gene p16(INK4a/CDKN2), a specific inhibitor of the cyclin-dependent kinases CDK4 and CDK6, is the most common genetic alteration in human pancreatic cancer, making it an ideal target for gene replacement. Here we transfected tumor cells using a recombinant adenovirus containing the wt-p16 cDNA (Ad5RSV-p16). The overexpression of p16 decreased cell proliferation in all four human pancreatic tumor cell lines (NP-9, NP-18, NP-29, and NP-31). However, G1 arrest and senescence were observed in only three. In contrast, the fourth (NP-18) showed a significant increase in apoptosis. This differential behavior may be related to the differences found in the expression level of E2F-1. Experiments on subcutaneous pancreatic xenografts demonstrated the effectiveness of p16 in the inhibition of pancreatic tumor growth in vivo. Taken together, our results ...
Recent evidence suggests a direct antiproliferative effect of LHRH agonists on the prostatic carcinoma cell line LNCaP. In the present study the possible presence of a LHRH degrading activity (LHRH-DA) in soluble fractions of LNCaP cell homogenates has been investigated. The results obtained show that an LHRH-DA is present in the soluble fraction of LNCaP cells with apparent Km and Vmax values of 31.6 mu M and 4.5 pmol/min/mu g protein respectively. The degradation pattern of LHRH is characterized by two major initial degradation products identified as LHRH 1-5 and LHRH 1-6 fragments. The degradation of the tracer [pGlu-H-3]LHRH, used as a substrate, is inhibited by synthetic unlabelled LHRH (IC50 7.9 mu M) and by several LHRH agonists with different kinetics and potencies; the LHRH agonist [DSer-(tBu)(6),Gly(10)-Aza]LHRH was the most potent blocker of LHRH-DA present in LNCaP cells; this enzymatic activity is also inhibited in a dose dependent manner by somatostatin, TRH, bacitracin and ...
We found that knocking down these transcripts in vitro with antisense oligonucleotide (ASO) induces selective death of several human and murine tumor cell lines without affecting viability of normal cells. The aim of this study was to evaluate in vitro and in vivo, the therapeutic value of silencing the ASncmtRNA with ASO in human cell lines/primary cultures and in A498 xenograft and Patient derived xenograft (PDX) RCC model.. Methods: For the in vitro studies we used different human renal carcinoma cell lines (786-O, Caki-1, A498) and 6 primary cultures derived from RCC patients. Knock down of the ASncmtRNA was achieved by transfection of ASO targeted to the loop region of these transcripts (Andes-1537). As control, we used ASO with unrelated sequence (NR-ASO). Cell death was evaluated with FACS by PI and tripan exclusion assay and the decrease in tumorigenicity was determined by sphere assay. The apoptosis hallmarks evaluated were Caspase activation, DNA fragmentation and phosphatidylserine ...
Detail záznamu - Neoplastic progression of the human breast cancer cell line G3S1 is associated with elevation of cytoskeletal dynamics and upregulation of MT1-MMP - Detail záznamu - Knihovna Akademie věd České republiky
To study potential sources of tumor-associated Ags in human ovarian cancer, we have established two ovarian tumor cell lines (OvS1 and OvA2) from two ovarian cancer patients, which express the cellular oncogene HER2/neu. Corresponding tumor infiltrating lymphocyte cultures have also been established and display an autologous tumor-specific pattern of cytotoxicity that is HLA-A2 restricted. To determine the potential relationship between HER2/neu expression and CTL-mediated cytolysis, we first established tumor cell clones from OvS1. These were categorized as high or low expressors of HER2/neu (cOvS1+ or cOvS1-, respectively), and cOvS1+ clones displayed a significantly higher sensitivity to CTL killing as compared with cOvS1- clones. To modulate the expression of HER2/neu, ovarian cancer cells were treated with IFN-gamma. After this exposure, HER2/neu expression was significantly decreased, whereas the expression of HLA Class I was significantly increased. Despite the increase in HLA Class I ...
The cyclin-dependent kinase member, Cdk5, is expressed in a variety of cell types, but neuron-specific expression of its activator, p35, is thought to limit its activity to neurons. Here we demonstrate that both Cdk5 and p35 are expressed in the human astrocytoma cell line, U373. Cdk5 and p35 are present in the detergent-insoluble cytoskeletal fraction of this cell line and Cdk5 localizes to filopodia and vinculin-rich regions of cell-matrix contact in lamellopodia. When exposed to a 46(o)C heat shock, U373 cells change shape, lose cell-matrix contacts and show increased levels of apoptosis. To test whether Cdk5 activation might play a role in these events, U373 cells were stably transfected with histidine-tagged or green fluorescent protein-tagged constructs of Cdk5 or a dominant negative mutation, Cdk5T33. Under normal growth conditions, growth characteristics of the stably transfected lines were indistinguishable from untransfected U373 cells and Cdk5 localization was not changed. However, ...
Amphiregulin (AR) is a newly discovered glycosylated, 84-amino acid residue polypeptide growth regulator which has sequence homology to the EGF family of proteins. To obtain immunological reagents to study the biological role of AR, two synthetic peptides containing sequences corresponding to distinct regions of AR were used to generate polyclonal antibodies in rabbits. One preparation of antipeptide antibodies directed against residues 26-44 of AR (AR-Ab2) was most effective in the detection of native AR, whereas another preparation of antibodies against residues 8-26 (AR-Ab1) was found to be most efficacious in the detection of AR in formalin-fixed and paraffin-embedded tissues. The growth of a colon carcinoma cell line, Geo, which proliferates autonomously under serum-free conditions, was stimulated by the exogenous addition of AR or EGF. Half-maximal stimulation of this growth was observed at 40 and 200 pM of EGF and AR, respectively. A mAb to the extracellular domain of the EGF receptor ...
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Additional comment: After the equilibration, the IPG gel strips were cut to size. Six mm were removed from the anodic end and 14 mm from the cathodic end. The second dimension gels were overlayered with a solution containing agarose (0.5% w/v) and Tris-glycine-SDS (25 mM-198 mM-0.1% w/v) pH 8.3 heated at about 70o C and the IPG gel strips were immediately loaded through it ...
Scale bar: 100 μm. B. The proliferation of atypical tumor cells with osteoid formation is shown. Xenografted tumor cells resemble original tumor cells. Scale bar: 50 μm. Cell growth and morphological findings in vitro UTOS-1 cells were spindle-shaped, contained several nucleoli, and formed clumps. Two weeks after initial cultivation in primary culture, the tumor cells reached subconfluence with some Pictilisib piled-up foci selleck products of cells (Figure 4A). After the cells were serially subcultured for about 3 months, they began to grow rapidly at passage 6 (Figure 4B). Figure 4 Morphology under phase-contrast microscopy. A. In primary. culture, spindle-shaped tumor cells reach subconfluence with some piled-up foci of cells. Scale bar: 100 μm. B. At passage. 6, the tumor cells begin to grow rapidly. The configuration of tumor cells is equalized after the 6th generation. Scale bar: 100 μm. This new cell line has been maintained in vitro for more than 50 passages over more than 2 years. ...
Single Cell Profiling of Circulating Tumor Cells: Transcriptional Heterogeneity and Diversity from Breast Cancer Cell Lines. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Ovarian cancer is a cancer that forms in an ovary. It results in abnormal cells that have the ability to invade or spread to other parts of the body. SK-OV-3, NIH:OVCAR-3 and HO-8910 are the ovarian carcinoma cell lines derived from human ovarian tumor. Studies were carried out to determin
MiRNA-21 ASO reduced the colony formation capacity of human colon carcinoma cells. Human colon carcinoma cell line HCT116 cells were transiently transfected wit
Rae-1 alpha, beta, gamma, δ and epsilon (retinoic acid early transcript 1) comprise a family of closely related (88‑95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection ...
TABLE-US-00004 TABLE 4 CTC CTPC Analysis of Cells Migrating from Tumor Cultures Cell Count CTC CTPC Test (Viable/ Clus- Clus- Group Sample Total) CTCs ters CTPCs ters 1 Day 2, 5, 7 ,1%/nd .sup. 70 4 44 0 Pool1,2 Day 9, 13, ,1%/nd .sup. 44 2 20 0 15 Pool1,2 Day 17, 19, 1360/12,600 0 0 4 2 21 Pool1,2 Day 23, 26, 1340/10,800 26 2 30 4 28 Pool1 Day 33 2,230/2,800.sup. 32 0 40 0 Day 35 1,700/8,500.sup. 54 0 46 0 Day 40 190/1,000 74 0 44 0 2 Day 2, 5, 7 ,1%/nd .sup. 166 0 76 2 Pool1,2 Day 9, 13, ,1%/nd .sup. 18 0 16 0 15 Pool1,2 Day 17, 19, 880/2,600 6 0 10 0 21 Pool1,2 Day 23, 26, 740/3,000 0 0 28 4 28 Pool1 Day 33 2,450/2,800.sup. 52 0 48 0 Day 35 1,420/3,500.sup. 62 0 50 0 Day 40 740/2,000 84 2 64 4 3 Day 2, 5, 7 ,1%/nd .sup. 80 0 74 0 Pool1,2 Day 9, 13, ,1%/nd .sup. 12 0 6 0 15 Pool1,2 Day 17, 19, 340/15,600 6 2 20 2 21 Pool1,2 Day 23, 26, 640/10,200 32 2 8 0 28 Pool1 Day 33 3,350/3,500.sup. 140 0 92 0 Day 35 980/2,000 46 0 34 0 Day 40 280/1,500 160 0 122 0 1Individual samples were frozen and ...
TY - JOUR. T1 - Experimental approaches to problems of invasion and metastasis.. AU - Paweletz, N.. AU - Paku, S.. AU - Werling, H. O.. AU - Spiess, E.. PY - 1986/1/1. Y1 - 1986/1/1. N2 - The highly metastasizing ASML cells and the non-metastasizing AS cells, arisen as spontaneous tumors of the rat, were confronted with rat lung tissue in vitro. Small cubes of the lung were allowed to heal their cut edges, then tumor cells were added. Both tumor cell types adapted their shape to the environment, penetrated the superficial layer of lung cells, either of epithelial or of fibroblastoid character and settled on the basal lamina, which, however, was not pierced. In a second set of experiments the tumor cells were inoculated intravenously into the living animal. The lung loaded with tumor cells was excised and cut into cubes which were then incubated in vitro. Here also both tumor cell types exhibited an invasive behavior but the basal lamina of the vessels in which the tumor cells have been arrested ...
The liver is a common site of metastases from a variety of organs such as lung, breast, colon and rectum. When liver metastases occur at the time of initial diagnosis of the primary tumor, they are described as synchronous. If detected after the initial diagnosis, they are described as metachronous. The liver is frequently involved since it receives blood from the abdominal organs via the portal vein. Malignant cells detach from the primary cancer, enter the bloodstream or lymphatic channels, travel to the liver, and grow independently. We do not understand the mechanism of how a tumor cell can leave the primary site and grow in specific organs. Potentially, the environment of the liver is suitable to the growth of certain tumor cells. Once a tumor begins to grow in the liver, it receives its blood supply from the hepatic artery ...
Oligonucleotides having approximately 8 to 18 nucleotide units and a 3-tail which includes asteroid structure attached to the 3-end through the A ring of the steroid skeleton and which form substantially stable duplexes at physiological temperature, have selective cytotoxic activity against certain tumor cell lines.
Buy our Raji (human Burkitts lymphoma cell line) membrane lysate, tumor cell line. ab30126 has been validated in western blot. Abcam now offers…
Cancer cell lines are originally derived from patient tumors, but acquire the ability to proliferate within in vitro cell cultures. As a result of in vitro manipulation, cell lines that have been traditionally used in cancer research undergo genetic transformations that are not restored when cells are allowed to grow in vivo.[9] Because of the cell culturing process, which includes enzymatic environments and centrifugation, cells that are better adapted to survive in culture are selected, tumor resident cells and proteins that interact with cancer cells are eliminated, and the culture becomes phenotypically homogeneous.[4] When implanted into immunodeficient mice, cell lines do not easily develop tumors and the result of any successfully grown tumor is a genetically divergent tumor unlike the heterogeneous patient tumor.[4] Researchers are beginning to attribute the reason that only 5% of anti-cancer agents are approved by the Food and Drug Administration after pre-clinical testing to the lack ...
Discover our worklow for the generation and culture of primary human tumor cell lines, and find out how to retain the primary tumors heterogeneity. - USA
HLDA WorkshopHCDM/HLDA 10Quantity100 testsVolume1.0 mLImmunogenDaudi human Burkitt lymphoma cell lineBackground InformationCD19 is a transmembrane gl…
HLDA WorkshopHCDM/HLDA 10Quantity100 testsVolume2.0 mLImmunogenDaudi human Burkitt lymphoma cell lineBackground InformationCD19 is a transmembrane gl…
Inhibitory effect on cultures tumor cell 5 Erythropoietin Kidney Stimulate development of erythropoietic cells ... Stimulate growth of mesenchymal cells, promotes wound healing 3 Transforming growth factor (Alpha) Epithelial cell Similar to ... cell membrane. Biological process. • negative regulation of epidermal growth factor receptor signaling pathway. • positive ... regulation of cell motility. • activation of MAPKK activity. • positive regulation of receptor internalization. • positive ...
Wright KO, Messing EM, Reeder JE (2004). "DBCCR1 mediates death in cultured bladder tumor cells". Oncogene. 23 (1): 82-90. doi: ... Perforin is stored in granules within cytotoxic T-cells and is responsible for killing virally infected and transformed cells. ... Perforin is released by cytotoxic T cells and lyses virally infected and transformed cells. In addition, perforin permits ... Cell. 121 (2): 247-56. doi:10.1016/j.cell.2005.02.033. PMID 15851031. S2CID 1613454. Huang Y, Qiao F, Abagyan R, Hazard S, ...
Wright KO, Messing EM, Reeder JE (2004). "DBCCR1 mediates death in cultured bladder tumor cells". Oncogene. 23 (1): 82-90. doi: ... Nishiyama H, Hornigold N, Davies AM, Knowles MA (Nov 1999). "A sequence-ready 840-kb PAC contig spanning the candidate tumor ... 2005). "Frequent silencing of DBC1 is by genetic or epigenetic mechanisms in non-small cell lung cancers". Hum. Mol. Genet. 14 ... Habuchi T, Yoshida O, Knowles MA (Jul 1997). "A novel candidate tumour suppressor locus at 9q32-33 in bladder cancer: ...
"WNT-4 mRNA expression in human adrenocortical tumors and cultured adrenal cells". Hormone and Metabolic Research. 40 (10): 668- ... Regardless of sex, though, WNT4 is needed for cell proliferation. In mouse gonads, it has been detected only eleven days after ... If deficient in XY mice, there is a delay in Sertoli cell differentiation. Moreover, there is delay in sex cord formation. ... For instance, TAFIIs 105 is now encoded, a subunit of the TATA binding protein for RNA polymerase in ovarian follicle cells. ...
"BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors". Journal of Proteomics. 118 (12): 95- ... cell cycle arrest. • positive regulation of response to DNA damage stimulus. • positive regulation of cell proliferation. • ... "Cell. 142 (3): 480-93. PMC 2923036 . PMID 20691906. doi:10.1016/j.cell.2010.06.037.. ... "Cell. 151 (1): 68-79. PMC 3471363 . PMID 23021216. doi:10.1016/j.cell.2012.08.033.. ...
The tumor was cultured in vivo, which became known as the Ehrlich cell. After 1948 Ehrlich cultures spread around research ... "Osmotic properties of ehrlich ascites tumor cells during the cell cycle". J Cell Physiol. 97 (3): 381-96. doi:10.1002/jcp. ... Dupre, Ann M.; Hempling, H. G. (December 1978). "Osmotic properties of ehrlich ascites tumor cells during the cell cycle". ... Culture techniques in large-scale, mice passage is less attractive, due to the contamination of the tumor with multifarious ...
"Type C RNA tumor virus isolated from cultured human acute myelogenous leukemia cells". Science. 187 (4174): 350-3. Bibcode: ... HL23V was reputedly a type C RNA tumor virus first isolated in 1975 from cultured human acute myelogenous leukaemia peripheral ... isolated from cultured acute myelogenous leukaemic cells". Nature. 260 (5548): 266-268. Bibcode:1976Natur.260..266C. doi: ...
"BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors". Journal of Proteomics. 118 (12): 95- ... Myc genes play a number of normal roles in stem cells including pluripotent stem cells. In neural stem cells, N-Myc promotes a ... There have been several studies that have clearly indicated Myc's role in cell competition. A major effect of c-myc is B cell ... In the experimental transformation process of normal cells into cancer cells, the MYC gene can cooperate with the RAS gene. ...
Cells from Lacks's tumor behaved differently. As was custom for Gey's lab assistant, she labeled the culture 'HeLa', the first ... and isolated one specific cell, multiplied it, and developed a cell line. Previously, cells cultured from other human cells ... HeLa cells are rapidly dividing cancer cells, and the number of chromosomes varied during cancer formation and cell culture. ... The total number of HeLa cells that have been propagated in cell culture far exceeds the total number of cells that were in ...
Turkey autopsies showed aflatoxins targeted the liver and either completely killed the tissue cells or induced tumor formation ... The culture was isolated, grown in pure culture, and a subset of healthy turkeys was infected. The pure culture isolate causes ... Rainbow trout are highly sensitive at 20 ppb, causing a liver tumor development in half the population. White rats develop ... The sexual state of this heterothallic fungus arises when strains of opposite mating type are cultured together. Sexual ...
Tokar, Steve (2005-09-02). "Omega-6 fatty acids cause prostate tumor cell growth in culture". Medical News Today. MediLexicon ... Mammalian cells lack the enzyme omega-3 desaturase and therefore cannot convert omega-6 fatty acids to omega-3 fatty acids, ... The conversion of cell membrane arachidonic acid (20:4n-6) to omega-6 prostaglandin and omega-6 leukotriene eicosanoids during ... A ratio of 2.5-to-1 reduced rectal cell proliferation in patients with colorectal cancer, whereas a ratio of 4-to-1 had no ...
Counting Actively Metabolizing Tissue Cultured Cells, Exp. Cell. Res., 1957, 13, 341-347. Masters R.W., Animal Cell Culture, ... Skehan P., Evaluation of Colorimetric Protein and Biomass Stains for Assaying Drug Effects Upon Human Tumor Cell Lines, Proc. ... Masters R.W., Animal Cell Culture, Cytotoxicity and Viability Assays, Third Edition, 202-203.. ... Culture of Animal Cells, A Manual of Basic Technique, 5th edition, Wiley-Liss, 200- 201. Mohanta T.K., Patra J.K., Rath S.K., ...
1983). "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells". Cell. 31 (1 ... keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells ... October 2004). "Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients ... 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ". Differentiation. 33 (1): 69-85. ...
Köhler G, Milstein C (July 1976). "Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion". ... tumor cell lines for the fusion. The thymidine kinase negative cells are obtained by growing the tumor cell line in the ... Tumor cells release enzyme to the circulation, probably in connection with the disruption of dead or dying tumor cells. The ... Hybridomas are cells obtained by fusing tumor cells (which can divide infinitely) and immunoglobulin-producing lymphocytes ( ...
For example, nelfinavir and atazanavir are able to kill tumor cells in culture (in a Petri dish).[11][12] This effect has not ... but studies in laboratory mice have shown that nelfinavir is able to suppress the growth of tumors in these animals, which ...
... the invasion of cancer cells into the lymphatic system). In vitro, when breast tumour cell lines with endothelins are ... Invasion through an artificial membrane can be stimulated, particularly when co-cultured in the presence of macrophages. The ... resulting in increased expression of ET-2 by breast tumour cells. This increased ET-2 expression provides the tumour with ... Grimshaw MJ (May 2005). "Endothelins in breast tumour cell invasion". Cancer Letters. 222 (2): 129-38. doi:10.1016/j.canlet. ...
"The critical effects of matrices on cultured carcinoma cells: Human tumor-derived matrix promotes cell invasive properties". ... are commonly used in cell culture systems to maintain stem and precursor cells in an undifferentiated state during cell culture ... Extracellular matrix proteins can also be used to support 3D cell culture in vitro for modelling tumor development. A class of ... Plant cells are tessellated to form tissues. The cell wall is the relatively rigid structure surrounding the plant cell. The ...
Laboratory studies with cultured tumor cells suggest that this activity may be the result of PP2A inhibition. Including broadly ... cellular structures involved in cell-to-cell adhesion, leading to detachment of the tonofilaments that hold cells together. The ... induces an oxidative stress-independent growth inhibition of pancreatic cancer cells through G2/M cell-cycle arrest and ... Dorn, D. C.; Kou, C. A.; Png, K. J.; Moore, M. A. S. (2009). "The effect of cantharidins on leukemic stem cells". International ...
... is a Trinidad and Tobago biophysicist researching the metastatic traits that allow tumor cells to colonize ... Tanner's expertise includes multimodal imaging platforms, 3D cell culture, biophysics, mechanobiology, and breast cancer. Her ... where she integrates concepts from molecular biophysics and cell biology to learn how cells and tissues sense and respond to ... laboratory focuses on understanding the metastatic traits that allow tumor cells to colonize secondary organs. Her team team ...
The protein is expressed in tissues throughout the body, but is absent or reduced in many kinds of tumor cells. While there are ... Its expression inhibits the growth of human breast and prostate cancer cells in culture. ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... is downregulated in cancer and inhibits tumor cell growth". Oncogene. 26 (31): 4580-9. doi:10.1038/sj.onc.1210244. PMID ...
In the study, investigators found that this combination destroyed up to 65 percent of cultured pancreatic tumor cells. The ... Panobinostat was able to selectively target triple negative breast cancer (TNBC) cells by inducing hyperacetylation and cell ... effect on HIV production in latently infected cells and T-cell activation". Human Vaccines & Immunotherapeutics. 9 (5): 993- ... These resting cells are not recognized by the immune system as harboring the virus and do not respond to antiretroviral drugs. ...
... adipocytes and especially in tumor cells. In tumor cells the citric acid cycle is truncated due to an inhibition of the enzyme ... Reitzer, LJ; Wice BM; Kennell D (1979). "Evidence that glutamine, not sugar, is the major energy source for cultured HeLa-cells ... "Tumor M2-PK and glutaminolytic enzymes in the metabolic shift of tumor cells". Anticancer Research. 20 (6D): 5151-5154. PMID ... Besides glycolysis in tumor cells glutaminolysis is another main pillar for energy production. High extracellular glutamine ...
... cells are most commonly used not as individual cells, but as a confluent monolayer on a cell culture insert filter (e.g ... PAMPA - a non cell-based permeability assay. References[edit]. *^ Fogh J and Trempe G in Human Tumor Cells In Vitro (J. Fogh, ... 2005). "The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell ... 1983). "Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture". Biol Cell. ...
The bioactive molecule betulin extracted from its bark has been shown to inhibit the growth of cultured human tumor cells. " ...
In mouse cell cultures, PD-L2 expression on tumor cells suppressed cytotoxic T cell-mediated immune responses. Indirectly, PD- ... 2016). "PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical ... PD-L2 is one of two known ligands for Programmed cell death protein 1 (PD-1). PD-L2 is a cell surface receptor belonging to the ... Others have shown PD-L2 expression in certain T helper cell subsets and cytotoxic T cells. PD-L2 protein is widely expressed in ...
Oct 15, 2008). "Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal". ... Experiments show that INH1 potently inhibits the proliferation of multiple human breast cancer cell lines, cervical HeLa cells ... INH1, a thiazolyl benzamide compound, is a cell-permeable Hec1/Nek2 mitotic pathway inhibitor I. INH1 controls the biological ... and colon cancer cells in vitro. "biological activity of INH1 by selleckchemicals". selleck. Wu G, et al. ( ...
Receptor CD44 participates in cell adhesion interactions required by tumor cells. Although hyaluronan binds to receptor CD44, ... Bacillus subtilis recently has been genetically modified to culture a proprietary formula to yield hyaluronans, in a patented ... directed migration and control of related cell mechanisms are mediated via the specific cell interaction between HA and cell ... By providing the dynamic force to the cell, HA synthesis has also been shown to associate with cell migration. Basically, HA is ...
"Grawitz' tumour", also known as renal cell carcinoma, is named after him. Geschichte der Medizinischen Fakultät Greifswald 1806 ... He is known for his pioneer work with tissue cultures, and his experimentation in the field of bacteriology. " ... see Renal cell carcinoma University of Greifswald v t e v t e. ...
Fruit body extracts have been shown to slow the growth of certain tumour cell lines in cell culture. Polish studies found that ... Tests with cell cultures and mice show that although it has some protective effect, it is toxic to cells in higher doses. A new ... The basidia (spore-bearing cells) are four-spored and measure 25-35 by 8-10 µm. Pleurocystidia (cystidia found on the faces of ...
... tumors, or traction. Primary cicatricial alopecias are further classified by the type of inflammatory cells that destroy the ... In addition, if pustules are present, cultures are taken to identify which microbes, if any, may be contributing to the ... If the stem cells and sebaceous gland are destroyed, there is then no possibility for regeneration of the hair follicle, and ... The goal of treatment is to decrease or eliminate the lymphocytic inflammatory cells that are attacking and destroying the hair ...
B. C. Giovanella u. a.: Development of invasive tumors in the nude mouse after injection of cultured human melanoma cells. In: ... D. Kong u. a.: Establishment and characterization of human pancreatic adenocarcinoma cell line in tissue culture and the nude ... L. Miers u. a.: Implantation of different malignant human cell lines in an athymic mouse does not alter success and growth ... T. Devos u. a.: Occurrence of autoimmunity after xenothymus transplantation in T-cell-deficient mice depends on the thymus ...
... and Th1 cells.[45] IL-1α stimulates increased skin cell activity and reproduction, which, in turn, fuels comedo development.[45 ... Society and culture[edit]. The costs and social impact of acne are substantial. In the United States, acne vulgaris is ... These gene candidates include certain variations in tumor necrosis factor-alpha (TNF-alpha), IL-1 alpha, and CYP1A1 genes, ... and accumulation of skin cells in the hair follicle.[1] In healthy skin, the skin cells that have died come up to the surface ...
... has been known to stimulate cell growth in normal and cancer cell line cultures,[37] and it was shown that ... a potential target for novel medicines in malignant brain tumour therapies (mini-review)". Folia Neuropathologica. 45 (3): 99- ... on cells (including cancer cells) bestowing upon them mobility.[40] and metastasis.[41] It has been suggested that cancer ... stem cells, white blood cells) in many tissues and organs. SP amplifies or excites most cellular processes.[15][16] ...
Graft-versus-tumor effect[edit]. Main article: Graft-versus-tumor effect. Graft-versus-tumor effect (GVT) or "graft versus ... during which neither showed traces of HIV in their blood plasma and purified CD4 T cells using a sensitive culture method (less ... a b Memorial Sloan-Kettering Cancer Center , Blood & Marrow Stem Cell Transplantation , The Graft-versus-Tumor Effect Archived ... who have lost their stem cells after birth. Other conditions[13] treated with stem cell transplants include sickle-cell disease ...
Society and culture[edit]. Economics[edit]. As of 2011, levothyroxine was the second most commonly prescribed medication in the ... It may also be used to treat and prevent certain types of thyroid tumors.[1] It is not indicated for weight loss.[1] ... T4 and T3 bind to thyroid receptor proteins in the cell nucleus and cause metabolic effects through the control of DNA ...
California Institute for Regenerative Medicine, Tumor Cells Become Drug Resistant by Reverting to a Stem Cell-Like State, New ... "Culture of Human Embryonic Stem Cells (hESC)". National Institutes of Health. Vaadatud 2013-10-21. ... Lindvall O (2003). "Stem cells for cell therapy in Parkinson's disease". Pharmacol Res 47 (4): 279-87. PMID 12644384. ... "Researchers find new method for turning adult cells into stem cells". Amherst Daily News. Canadian Press. 2009-01-03. Vaadatud ...
"Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment". The ... Lang F, Alevizopoulos K, Stournaras C (2013). "Targeting membrane androgen receptors in tumors". Expert Opin. Ther. Targets. 17 ... The mesoderm-derived epithelial cells of the sex cords in developing testes become the Sertoli cells, which will function to ... These are Leydig cells. Soon after they differentiate, Leydig cells begin to produce androgens. ...
In 1975, association with "HL-A1,8" (Current name: HLA A1-B8) was confirmed by serological typing of cells from myasthenics.[11 ... Most important of which is the TNF (tumor necrosis factors) with 3 loci in the region. Starting from B8, immediately followed ... but because it was less selective in African holocene relative to European holocene climate/culture, the haplotype underwent ... June 2004). "Genetic polymorphisms in tumor necrosis factor (TNF)-alpha and TNF-beta in a population-based study of systemic ...
... cells in culture". Journal of Steroid Biochemistry. 31 (5): 845-52. doi:10.1016/0022-4731(88)90295-6. PMID 2462135.. ... Lang F, Alevizopoulos K, Stournaras C (2013). "Targeting membrane androgen receptors in tumors". Expert Opin. Ther. Targets. 17 ... Cell Biol. 42 (6): 813-27. doi:10.1016/j.biocel.2009.11.013. PMID 19931639.. ... Bennett NC, Gardiner RA, Hooper JD, Johnson DW, Gobe GC (2010). "Molecular cell biology of androgen receptor signalling". Int. ...
... or the virus itself in cell culture.[1] Other conditions that may present similarly include Ebola, malaria, typhoid fever, and ... These tests include cell cultures, PCR, ELISA antigen assays, plaque neutralization assays, and immunofluorescence essays. ... Other laboratory findings in Lassa fever include lymphocytopenia (low white blood cell count), thrombocytopenia (low platelets ...
Cells, circulating tumor cells (CTCs), or formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections are fixed, then ... "Defining the sister rat mammary tumor cell lines HH-16 cl.2/1 and HH-16.cl.4 as an in vitro cell model for Erbb2". PLOS ONE. 7 ... in cells, circulating tumor cells, and tissue samples. In this context, it can help define the spatial-temporal patterns of ... FISH can also be used to detect diseased cells more easily than standard Cytogenetic methods, which require dividing cells and ...
When the bladder is fully distended the superficial cells become squamous (flat) and the stratification of the cuboidal cells ... In Western culture, the standing position is regarded by some as superior and more masculine than the sitting or squatting ... When the afferent and efferent nerves are both destroyed, as they may be by tumors of the cauda equina or filum terminale, the ... Depending on the culture, adult women, unlike men, are restricted in where they can urinate.[32] ...
If acute radiation exposure occurs in the first ten days following conception, when few cells have formed, it is likely that ... other solid tumor cancers, and leukemia. The relationship between radiation exposure and subsequent cancer risk is considered " ... culture, occupation, class, location, and simultaneous exposure to additional environmental toxins are also significant, but ... leukemia and certain solid tumors that have developed within a decade or more after exposure. As studies of biological samples ...
Partridge, Christopher (2004). The Re-enchantment of the West: Alternative Spiritualities, Sacralisation, Popular Culture, and ... it was reported that he was being treated for a brain tumour at a clinic in Switzerland.[196] While in Switzerland, Starr ... he began radiotherapy at Staten Island University Hospital in New York City for non-small cell lung cancer that had spread to ... By the mid-1960s Harrison had become an admirer of Indian culture and mysticism, introducing it to the other Beatles.[350] ...
... and interleukin-1 in modulating progesterone and oestradiol production by human luteinized granulosa cells in culture. Hum. ... Fukuoka M, Yasuda K, Fujiwara H, Kanzaki H, Mori T. Interactions between interferon gamma, tumour necrosis factor alpha, ... Is Mediated by Phosphatidyl Inositol 3-Kinase But Not Extracellular Signal-Regulated Kinase-1/2 in Human Ovarian Theca Cells. ...
Follicular dendritic cell sarcoma. Extranodal NK/T-cell lymphoma, nasal type. MCPyV Merkel-cell carcinoma. RNA virus. HCV ... A map of the genome of JC virus, indicating the position of the tumor antigen genes (red), the three capsid protein genes ( ... and later isolated in culture and named using the two initials of a patient, John Cunningham, with progressive multifocal ... For example, JCV has been found to infect the granule cell layer of the cerebellum, while sparing purkinje fibers, ultimately ...
"for their discoveries concerning the interaction between tumour viruses and the genetic material of the cell"[۶۱] ... "for the human understanding and subtle analysis of contemporary culture that are combined in his work"[۶] ... "for their discovery of جی پروتئینs and the role of these proteins in ورارسانی پیام in cells"[۷۳] ... for "their discoveries of machinery regulating vesicle traffic, a major transport system in our cells"[۸۱] ...
T suppressor cells - T4 cell - T4 cells (T-helper cells) - T8 cells - Tanner staging - TAT - TB - template - TeachAIDS - ... viral culture - viral envelope - viral load - viremia - viricide - virion - virology - virus - visceral ... tumor necrosis factor (TNF) ... B-cell lymphoma - B cells - B lymphocytes (B cells) - ... cells - CDC National Prevention Information Network (CDC-NPIN) - cell lines - cell-mediated immunity (CMI) - cellular immunity ...
This is carried out by using donor-derived antigen-presenting cells. These new methods have reduced culture time to 10-12 days ... Immune deficiencies can result in persistent or recurring infections, autoinflammatory disorders, tumors, and disorders of ... cell responses to mitogens and allogeneic cells, cytokine production by cells Tests for B cell function: antibodies to routine ... natural killer cells and monocytes (CD15+), as well as activation markers (HLA-DR, CD25, CD80 (B cells). Tests for T cell ...
Kim Y, Berrios GE (2001). "Impact of the term schizophrenia on the culture of ideograph: the Japanese experience". Schizophr ... but clozapine can lower the white blood cell count in 1 to 4 percent of people who take it. This is a serious side effect.[16][ ... A stroke or brain tumor. *A metabolism (chemical reactions keeping the person alive) that is too high or too low ... 1992). "Schizophrenia: manifestations, incidence and course in different cultures. A World Health Organization ten-country ...
In popular cultureEdit. The book The Paris Option by Robert Ludlum and Gayle Lynds begins with four men blowing up the Institut ... The phagocytosis theory is based on the notion that phagocytes are cells that have the power to englobe foreign bodies - and ... Yersin looked for the germ responsible for the infection specifically in these plague-spots, tumors caused by the inflammation ... Albert Calmette and Camille Guérin discovered how to culture the tuberculosis bacillus, Mycobacterium tuberculosis (so called ...
B cell activation, and cell-mediated cytoxicity.[citation needed] Tumor development is a complex process that requires cell ... Evidence from cell lines and primary breast cancer cultures in vitro". Angiogenesis. 8 (3): 197-204. doi:10.1007/s10456-005- ... Paracrine signaling is a form of cell-cell communication in which a cell produces a signal to induce changes in nearby cells, ... Moreover, it was shown that tumor progression selects for cells that are VEGF-dependent, challenging the belief that VEGF's ...
Prion-like propagation of misfolded proteins from cell to cell may explain why ALS starts in one area and spreads to others.[27 ... Society and culture[edit]. See also: List of people with amyotrophic lateral sclerosis ... such as a spinal cord tumor, multiple sclerosis, a herniated disk in the neck, syringomyelia, or cervical spondylosis.[4] ... One 2016 review of stem-cell therapy trials found tentative evidence that intraspinal stem cell implantation was relatively ...
... tumor activity in cell culture, inducing apoptosis by disrupting the regulated degradation of pro-growth cell cycle proteins.[ ... some cell lines - in particular, primary cultures of quiescent and differentiated cells such as thymocytes and neurons - are ... for B-cell acute lymphoblastic leukemia [109] Proteasome inhibitors can kill some types of cultured leukemia cells that are ... Gankyrin is anti-apoptotic and has been shown to be overexpressed in some tumor cell types such as hepatocellular carcinoma.[81 ...
2000). "Elastic fiber proteins in the glomerular mesangium in vivo and in cell culture". Kidney Int. 58 (4): 1588-602. doi: ... 1999). "EMILIN, a component of the elastic fiber and a new member of the C1q/tumor necrosis factor superfamily of proteins". J ... 2003). "beta 1 Integrin-dependent cell adhesion to EMILIN-1 is mediated by the gC1q domain". J. Biol. Chem. 278 (8): 6160-7. ... Cell. Proteomics. 5 (2): 226-33. doi:10.1074/mcp.M500324-MCP200. PMID 16263699.. ...
"T cell receptor-dependent cell death of T cell hybridomas mediated by the CD30 cytoplasmic domain in association with tumor ... I. Partial characterization of soluble Ki-1 antigen and detection of the antigen in cell culture supernatants and in serum by ... CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. ... Various types of CD30-positive T cell lymphomas[11]. *CD30-positive cases of the NK cell lymphoma, extranodal NK/T-cell ...
... to kill tumor cells. The same literature claims that ascorbic acid acts as an antioxidant, thereby reducing the adverse effects ... Society and culture[edit]. In February 2011, the Swiss Post issued a postage stamp bearing a depiction of a model of a molecule ... Vitamin C distributes readily in high concentrations into immune cells, has antimicrobial and natural killer cell activities, ... Cell. 132 (6): 1039-48. doi:10.1016/j.cell.2008.01.042. PMID 18358815. Lay summary - Science Daily (March 21, 2008).. ...
Tumor cell survival[edit]. Alternatively, autophagy has also been shown to play a huge role in tumor cell survival. In ... as well as that of nutrient starved cultured cells and tissues.[59][60] Mutant yeast cells that have a reduced autophagic ... Mechanism of cell death[edit]. Cells that undergo an extreme amount of stress experience cell death either through apoptosis or ... There is evidence that emphasizes the role of autophagy both as a tumor suppressor as well as a factor in tumor cell survival. ...
CulturedTumor Cells, CulturedCell Line, TumorA549 CellsCaco-2 CellsHCT116 CellsHeLa Cells +Hep G2 CellsHL-60 CellsHT29 Cells ... Tumor Cells, Cultured. Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can ... Jurkat CellsK562 CellsPC12 CellsTHP-1 CellsU937 CellsEmbryonal Carcinoma Stem Cells ... All MeSH CategoriesAnatomy CategoryCellsCells, ... Cultured Tumor Cells. *Cell, Cultured Tumor. *Cells, Cultured ...
Anatomical context of Tumor Cells, Cultured. *The absence of 3,6-isoLD1 in cultured tumor cells of the central nervous system ... The successful surface expression of CD80 was obtained in both cultured tumor cell lines and primary cultured tumor cells [35]. ... Analytical, diagnostic and therapeutic context of Tumor Cells, Cultured. *We also tested fresh or newly cultured tumor cells ... These cells were lytic to cultured tumor cell lines, yet sparing normal primary cell cultures as has been reported [40]. ...
Isolation and Culture Expansion of Tumor-specific Endothelial Cells, An In Vitro System to Study Tumor Dormancy and the ... Capture and Release of Viable Circulating Tumor Cells from Blood, A Brain Tumor/Organotypic Slice Co-culture System for ... In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis, An Enzyme- and Serum-free Neural Stem Cell Culture Model for ... Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for ...
... by which some genetically deregulated and aggressive tumour cells generat ... Vascuologenesis is the de novo establishment of blood vessels and vascular networks from mesoderm-derived endothelial cell ... lined by tumour cells alone, without endothelial cells. Including examination of the cultured cell networks for PAS matrix or ... 1] in their investigation of ocular malignant melanomas, studying the tumour cells both cultured in vitro and in tumour tissue ...
Isolated tumor endothelial cells maintain specific character during long-term culture.. Matsuda K1, Ohga N, Hida Y, Muraki C, ... Increasing evidence indicates that tumor endothelial cells (TECs) are more relevant to the study of tumor angiogenesis than ... Stem cell antigen-1 was up-regulated in all four TECs, suggesting that they have a kind of stemness. Cultured TECs maintain ... However, it is challenging to isolate and culture large numbers of pure ECs from tumor tissue since the percentage of ECs is ...
Experiences with the human tumor stem cell assay(HTSCA) & its modifications for culturing gynaecological tumors. ...
If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. ... Tumor Cells, Cultured. Known as: Cultured Neoplastic Cells, Neoplastic Cells, Cultured, Neoplastic Cell, Cultured Expand. ... Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell ... Myeloid‐derived suppressor cells (MDSCs) promote tumor progression. The mechanisms of MDSC development during tumor growth ...
... expanding in culture, and freezing circulating tumor cells ... Development of an Ex Vivo Device Kit for Circulating Tumor Cell ... A Pilot Study to Facilitate Development of an Ex Vivo Device Kit for Circulating Tumor Cell Harvesting, Banking, and Apoptosis- ... stage III cancer of any solid tumor type. Patients may have been previously treated. or chemo naïve.. - Age > 18yo. - Life ...
Gastrointestinal Tumor Antigen from human cell culture for your research needs. Find product specific information including CAS ... Gastrointestinal Tumor Antigen from human cell culture ≥10,000 units/mL, buffered aqueous solution Synonym: Cancer-associated, ... Cancer Markers and Antibodies, Cancer Research, Cell Biology, Cell Signaling and Neuroscience, Immune Cell Signaling and Blood ...
... b tumor cell migration after 24 h in control culture, c tumor cell migration after 24-h incubation with L-arginine, d tumor ... 0.05-a co-culture of tumor/normal cells compared to an appropriate monoculture of normal cells. # p ≤ 0.05-a culture of tumor ... 0.05-a co-culture of tumor/normal cells compared to an appropriate monoculture of normal cells. # p ≤ 0.05-a culture of tumor ... Cell Migration Assessment. Tumor cells were plated at 5 × 105 cells/ml on 4 cm culture dishes (Nunc). After formation of ...
... has demonstrated that omega-6 fatty acids such as the fat found in corn oil promote the growth of prostate tumor cells in the ... Omega-6 fatty acids cause prostate tumor cell growth in culture; potential drug target identified By Steve Tokar ... When introduced into prostate tumor cells in culture, omega-6 fatty acid causes the production of cPLA2, which then causes the ... Working with human prostate cancer cells in tissue culture, Hughes-Fulford and her fellow researchers identified for the first ...
... and prostate tumor cell lines DU-145, LNCaP, PC-3, and HPC8L (established in our laboratory from a tumor resected from an ... Treated prostate tumor cell lines trended toward apoptosis-induction, as evident in bcl-xl/bax ratios ... cell growth inhibition assays were used to evaluate the proteins growth inhibitory activity against prostate tumor cells; and ... to inhibit prostate tumor cell growth in vitro. This study investigates the effect of eosinophil MBP extract on the expression ...
3D culture broadly regulates tumor cell hypoxia response and angiogenesis via pro-inflammatory pathways.. [Peter DelNero, ... Microarray gene expression analysis of tumor cells cultured in 2D versus 3D under ambient or hypoxic conditions revealed ... unbiased data sets describing tumor cell gene expression as a function of oxygen levels within three-dimensional (3D) culture. ... Here, we utilized alginate-based, oxygen-controlled 3D tumor models to study the interdependence of culture context and the ...
Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586. Toby G. Rossman, Lawrence Prutkin and Victor R. Wheatley ... Cells from the mouse preputial gland tumor ESR 586 have been cultured and cloned. Trypsin-ethylenediaminetetraacetate was used ... Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586 ... Culture of Cloned Cells from the Mouse Preputial Gland Tumor ESR 586 ...
Interaction of cholera enterotoxin with cultured adrenal tumor cells.. R M Wishnow, E Lifrak, C Chen ... Interaction of cholera enterotoxin with cultured adrenal tumor cells. Message Subject (Your Name) has forwarded a page to you ... In the adrenal tumor cell system ganglioside Gm1 inhibited cholera enterotoxin (CT)-induced steroidogenesis if it was ... Further studies showed that pretreatment of cultured adrenal cells with a maximal dose of CT increased cyclic adenosine 3-5- ...
... in order to track ongoing genetic mutation within a tumor. ... created a microchip device that can capture circulating tumor ... Cultured circulating tumor cells reveal genetic profile of breast cancer cells Circulating tumor cells captured with a ... Circulating tumor cells (CTCs) are living solid-tumor cells that separate from either primary or secondary tumors and go on to ... Cultured circulating tumor cells reveal genetic profile of breast cancer cells. Share this content: *facebook ...
These formulations generally comprise hybridoma of at least one antigen presenting cell ... Formulations comprising combinations of APCs and tumor cells and APCs and virally infected cells are disclosed. ... Day 6 dendritic cells were then used to form a co-culture with either B16 cells or 3LL cells. Each DC/tumor cell co-culture was ... here APCs and either tumor cells or virally infected cells. A co-culture of APCs and either tumor cells or virally infected ...
Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a ... 116 human colon cancer cell line. Reporter activity in HCT-116 cells transiently transfected with the various ABCG2 promoter ...
Adverse Cell Culture Conditions Mimicking the Tumor Microenvironment Upregulate ABCG2 to Mediate Multidrug Resistance and a ... Cell Culture and Growth Conditions. Human colon carcinoma HCT-116, and S1 and its ABCG2-overexpressing resistant S1M1-80 cell ... It is also believed to be a survival factor for cancer stem cells to drive tumor growth. Tumor microenvironment represents an ... ΔΔCt was then calculated by subtracting the ΔCt of the untreated cells (or parental cells) from the ΔCt of the treated cells ( ...
We first investigated the effects of cell source and culture conditions on lung colony formation by i.v. injected B16a (B16 ... cells and inhibition of tumor colony formation by the thromboxane A2 synthase ... The present study was undertaken to investigate the factors involved in determining the metastatic potential of cultured cells ... Phenotypic properties of cultured tumor cells: integrin alpha IIb beta 3 expression, tumor-cell-induced platelet aggregation, ...
IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. ... IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. ... IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. ... IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. ...
A Small-Molecule Modulator of Poly-α2,8-Sialic Acid Expression on Cultured Neurons and Tumor Cells ... A Small-Molecule Modulator of Poly-α2,8-Sialic Acid Expression on Cultured Neurons and Tumor Cells ... A Small-Molecule Modulator of Poly-α2,8-Sialic Acid Expression on Cultured Neurons and Tumor Cells ... A Small-Molecule Modulator of Poly-α2,8-Sialic Acid Expression on Cultured Neurons and Tumor Cells ...
... isolated and cultured from murine blood, demonstrate a distinct response to hypoxia compared with parental MDA-MB-231cells. (Ai ... fig3: Circulating tumour cells (CTCs), isolated and cultured from murine blood, demonstrate a distinct response to hypoxia ... fig3: Circulating tumour cells (CTCs), isolated and cultured from murine blood, demonstrate a distinct response to hypoxia ... Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype. ...
Figure 2: Induction of apoptosis in diffuse large B-cell lymphoma cell line U-2932 by etoposide treatment. Cells are labeled ... Applicability of FBS alternatives for sustainable and reproducible serum-free cell culture (a collection-related project in ... Malignant hematopoietic cell lines: in vitro models for double-hit B-cell lymphomas. Leukemia Lymphoma. 2016; 57(5):1015-20. ... Genomic Landscape of Primary Mediastinal B-Cell Lymphoma Cell Lines. PLoS One. 2015; 10(11):e0139663. ...
... and related methods of evaluating the potential responsiveness of the tumor cells and the patient to one or more therapeutic ... Provided are suspension-based cell culture systems and media for the timely and efficient proliferation of human tumor cell ... TUMOR CELL SUSPENSION CULTURES AND RELATED METHODS. Abstract:. Provided are suspension-based cell culture systems and media for ... and related methods of evaluating the potential responsiveness of the tumor cells and the patient to one or more therapeutic ...
Circulating tumor cells (CTCs), which are shed from the primary tumor into the circulation, serve as precursors of metastasis. ... positive cells revealed proliferating cell population in the 3D scaffolds. The CTCs cultured without prior enrichment exhibited ... Here we present a novel ex vivo 3D system for culturing CTCs from breast cancer patient blood samples using porous poly(ε- ... As a proof of principle study, we show that ex vivo culture of 12/16 (75%) advanced stage breast cancer patient blood samples ...
... tumor cells must overcome glucose deprivation. Accordingly, tumor cells depend on cellular pathways promoting survival under ... As such, we sought to identify small-molecule inhibitors which sensitize tumor cells to glucose starvation by high-throughput ... Specifically, we searched for inhibitors that selectively killed tumor cells growing in glucose-free but not in normal medium. ... The efficacies of two of the identified compounds, QNZ (EVP4593) and papaverine, were validated in four different tumor cell ...
... and the adherence of cells to plastic were studied in cultured human A549 lung tumor cells. The density of beta AR, assayed by ... The results indicate that multiple effects of IL-1 in a cultured tumor cell line involve different mechanisms, suggesting ... IL-1 inhibited cell proliferation and thymidine uptake, and increased the adherence of A549 cells to the plastic culture flask ... and differentiation in cultured human A549 lung tumor cells.. T Nakane, T Szentendrei, L Stern, M Virmani, J Seely and G Kunos ...
For gene expression quantification of CX 43, 32 and E-cadherin in canine mast cell tumors (MCT), 95.5% of samples were ... cell culture; breakdown for cytogenetic analysis of chromosomes (based on the Boxer breed published genome); Cell lysis for ... Animals: Thirty-six dogs with mast cell tumors. Procedures: The samples were submitted for the following analyses: stereology; ... Real Time PCR and quantification of gene expression of CX 43, 32 and E-cadherin in canine mast cells. Results: Cytogenetic ...
... breast tumor cell lines MCF-7, T-47D, and colon tumor cell line HCT-8). Insulin receptors on breast tumor cells were resistant ... The sensitivity of insulin receptors to down regulation by insulin has been measured in cultured human tumor cells ( ... Down Regulation and Dissociation of Down Regulation from Insulin Biological Action in Cultured Human Tumor Cells. Kathleen G. ... Maximum down regulation of receptors in all three types of tumor cell by prior exposure to insulin did not significantly alter ...
  • Cells grown in vitro from neoplastic tissue. (nih.gov)
  • We have investigated eosinophils as potential anti-cancer effector cells, and have reported the ability of their toxic granular proteins (MBP, EPO, ECP, EDN) to inhibit prostate tumor cell growth in vitro . (scirp.org)
  • 14. The formulation of claim 13, wherein said dendritic cells are selected from the group consisting of cutaneous epidermal Langerhans cells, dermal dendritic cells, lymph node dendritic cells, spleen dendritic cells and dendritic cells derived through in vitro culture of precursors. (freepatentsonline.com)
  • Overexpression of ABCG2 in cancer cell lines in vitro has been shown to confer MDR to a variety of anticancer drugs including mitoxantrone, irinotecan, methotrexate, flavopiridol, and anthracyclines [ 1 ]. (hindawi.com)
  • In addition, treatment with IL-6 resulted in a reduction of monocyte in vitro cytotoxicity for tumor target cells. (jimmunol.org)
  • Malignant hematopoietic cell lines: in vitro models for double-hit B-cell lymphomas. (dsmz.de)
  • Drexler HG, Ehrentraut S, Nagel S, Eberth S, MacLeod R: Malignant hematopoietic cell lines: In vitro models for the study of primary mediastinal B-cell lymphomas. (dsmz.de)
  • As such, we sought to identify small-molecule inhibitors which sensitize tumor cells to glucose starvation by high-throughput drug screening in vitro. (mdpi.com)
  • Reproducible three-dimensional in vitro systems for exploring interactions of the stroma with pancreatic cancer cells have not previously been available, prompting us to develop such a model. (nih.gov)
  • In conclusion, pancreatic organotypic culture offers a reproducible, bio-mimetic, three-dimensional in vitro model that allows examination of the interactions between stromal elements and pancreatic cancer cells. (nih.gov)
  • The effectiveness of these derivatives in causing photo-killing of tumor cells in vitro, as determined by colony forming assay, correlated well with their singlet oxygen yields indicating that this is likely to be the main mechanism for the photocytotoxicity. (spie.org)
  • Tumor necrosis factor (TNF), a potent inflammatory mediator secreted by monocytes during inflammation, was shown to significantly increase the adherence of Staphylococcus aureus to cultured human umbilical vein endothelial cells in vitro. (asm.org)
  • Mechanosensitivity of different cancer cells in vitro are investigated primarily with immortalized cell lines or murine derived primary cells, not with primary human cancer cells. (jove.com)
  • Hence, little is known about the mechanosensitivity of primary human colon cancer cells in vitro . (jove.com)
  • ccRCC primary cultures are a reliable in vitro model, well-reproducing original tumor genetics and phenotype, potentially useful for future functional approaches aimed to study genes or pathways involved in ccRCC etiopathogenesis and to identify novel clinical markers or therapeutic targets. (biomedcentral.com)
  • This study underpins various factors that cause resistance to anticancer drugs in forms of spheroids in 3D in-vitro cell culture and also outlines key challenges and possible solutions for the future development of these systems. (eurekaselect.com)
  • Jaroch K, Jaroch A, Bojko B. Cell cultures in drug discovery and development: the need of reliable in vitro-in vivo extrapolation for pharmacodynamics and pharmacokinetics assessment. (eurekaselect.com)
  • Three-dimensional in vitro cell culture models in drug discovery and drug repositioning. (eurekaselect.com)
  • A novel three-dimensional stromal-based model for in vitro chemotherapy sensitivity testing of leukemia cells. (eurekaselect.com)
  • DMP1 was isolated in a yeast two-hybrid interactive screen performed with cyclin D2 as bait, and the protein binds to any of the three D-type cyclins, but not to cyclins A, B, C, or H in vitro or when expressed with them in insect Sf9 or mammalian cells ( 26 , 27 ). (pnas.org)
  • As linear correlations exist between expression levels of angiogenic factors under normoxic and hypoxic conditions in vitro , we propose that explanted primary cells may be used to probe the in vivo hypoxic environment. (biomedcentral.com)
  • In vitro studies have shown that stromal cells cultured in hypoxic growth conditions secrete higher levels of critical angiogenesis-inducing factors than cells cultured in normoxic conditions [ 3 ]. (biomedcentral.com)
  • found hypoxia induced a 13-fold increase in the number of KDR receptors per endothelial cell in vitro , which may be the mechanism of action for the pronounced effect of hypoxia and VEGF in vivo [ 12 ]. (biomedcentral.com)
  • This study indicates that inhibitors influence lymphocyte blastogenic responses to tumor cells in vitro and suggests that such factors may influence host response to tumors. (elsevier.com)
  • Taken together, the data indicate that flavopiridol has activity against glioma cell lines in vitro and should be considered for clinical development in the treatment of glioblastoma multiforme. (aacrjournals.org)
  • It induces growth arrest at either the G 1 and/or G 2 phases of the cell cycle in numerous cell lines in vitro by acting as a competitive binding agent for the ATP-binding pocket of CDK ( 18 ). (aacrjournals.org)
  • After culture in the presence of IL-2, NKT cells can mediate lysis through TCR, NK1.1, or CD16, and kill classical NK-sensitive targets such as YAC-1 in vitro ( 23 )( 24 ). (rupress.org)
  • 9,10 Previous in vitro evidence showed that carvedilol, as an oxidant scavenger, could inhibit cardiomyocyte membrane lipid peroxidation and protect cells from oxidant injury. (ahajournals.org)
  • 9,12 Recently, carvedilol was shown to reduce low-density lipoprotein oxidation in vitro 13,14 and inhibit smooth muscle cell migration, proliferation, or neointimal formation after vascular injury in vivo. (ahajournals.org)
  • We then tested the hypothesis that carvedilol may reduce tumor necrosis factor-α (TNF-α)-induced intracellular ROS production, redox-sensitive transcription pathway activation, and adhesion molecules expression, resulting in the inhibition of endothelial adhesiveness to human MNCs in vitro-an early sign of atherogenesis. (ahajournals.org)
  • We also confirmed that miR-17 exerted this function by directly targeting RND3 in vitro , and that the expression of miR-17 was negatively correlated with that of RND3 in CRC tissues and CRC cells. (portlandpress.com)
  • Further in vitro migration assays were performed to confirm cancer cells conditioned medium dependent MSC and doxorubicin (DOX) treated MSC migration. (medsci.org)
  • MSCs were loaded with DOX, and their therapeutic effects against the cancer cells were studied in vitro . (medsci.org)
  • ectopic S1PR2 expression induces apoptosis in DLBCL cells in vitro and prevents tumor growth. (bloodjournal.org)
  • We found that in vitro Notch pathway blockade in glioblastoma (GBM) cells using a gammasecretase inhibitor (GSI) led to increased activity in two other pathways important for neural development-Wnt and Hedgehog. (aacrjournals.org)
  • Inhibition of both Notch and Hedgehog in vitro dramatically decreased the growth of GBM cell lines and low-passage neurospheres derived from primary human tumors. (aacrjournals.org)
  • This study investigates the effect of eosinophil MBP extract on the expression of oncogenes p53, bcl-xl, bax, and c-myc, which modulate tumor growth, proliferation, and apoptosis. (scirp.org)
  • These preliminary results demonstrate the growth inhibitory potential of eosinophil granular proteins and strongly support the hypothesis that eosinophils modulate the expression of oncogenes associated with prostate tumor proliferation and apoptosis. (scirp.org)
  • Both GM-CSF or IL-6 inhibited [3H]TdR uptake in U937 cells, and simultaneous treatment with GM-CSF and IL-6 resulted in an additive inhibitory effect on cell proliferation. (jimmunol.org)
  • Effects of IL-1 and cortisol on beta-adrenergic receptors, cell proliferation, and differentiation in cultured human A549 lung tumor cells. (jimmunol.org)
  • The effects of IL-1 and cortisol, and their interactions on the density of beta-adrenergic receptors (beta AR), cell proliferation, and the adherence of cells to plastic were studied in cultured human A549 lung tumor cells. (jimmunol.org)
  • IL-1 inhibited cell proliferation and thymidine uptake, and increased the adherence of A549 cells to the plastic culture flask, as quantified by a cell detachment assay. (jimmunol.org)
  • Provided are suspension-based cell culture systems and media for the timely and efficient proliferation of human tumor cell clusters from a patient, and related methods of evaluating the potential responsiveness of the tumor cells and the patient to one or more therapeutic agents. (wipo.int)
  • Tumor cells utilize glucose to fuel their anabolic needs, including rapid proliferation. (mdpi.com)
  • Administering these drug combinations (i.e., avastin and papaverine, and avastin and QNZ) led to significant reductions in proliferation and mTOR activity of the aggressive DLD1 colon cell line in mice. (mdpi.com)
  • In three-dimensional reconstituted basement membrane culture, they undergo a well-defined program of proliferation, differentiation, and growth arrest, forming acinar structures that recapitulate many aspects of mammary architecture in vivo. (biomedsearch.com)
  • Features of tissue morphogenesis were assessed, including proliferation, basement membrane formation, polarization of alpha-6 beta-4 integrin to the basement membrane, formation of cell:cell junctions, and apoptosis for luminal clearance. (biomedsearch.com)
  • Universal features of breast cancer include a loss of control of cell proliferation and organization, a loss of cell polarity, as well as a loss of cell: cell adhesion and cell: basement membrane adhesion. (biomedsearch.com)
  • Proliferation and apoptosis, as well as important cell-cell adhesion and cytoskeleton-regulating proteins, were studied. (nih.gov)
  • This virus activates the cellular retinoblastoma oncoprotein and cell cycle machinery, triggering continual cellular proliferation. (scirp.org)
  • JA was used in the concentration of 2 mM, as it suppressed cell proliferation in both cell lines. (springer.com)
  • Functionality of these monocytes and their ability to influence T cell phenotype and proliferation was investigated. (uni-wuerzburg.de)
  • The retention of abundant basement membrane molecules in the stromal space that includes pseudocystic cavities suggests that basement membrane matrix is essential for proliferation of adenoid cystic carcinoma cells. (nii.ac.jp)
  • In addition to its contribution to arsenite resistance, ARS2 is noteworthy for its role in mammalian development, modulation of cell proliferation, and promotion of the accumulation of several micro RNAs (miRNAs) involved in cellular transformation and inflammation 12 , 13 . (nature.com)
  • Results Significant reductions in cellular senescence were observed in tumour versus non-tumour cultures, accompanied by a stepwise increase in proliferation:senescence ratios. (lenus.ie)
  • Conclusions Our results suggest that an imbalance in tumour progenitor subpopulations imbalances the functional relationship between proliferation and senescence, creating a microenvironment favouring tumour progression. (lenus.ie)
  • At the same time, TET1 of glioma U251 cells was knocked down or overexpressed to observe its effect on glioma cell proliferation and invasion as well as autophagy level. (portlandpress.com)
  • When TET1 gene in glioma U251 cells was knocked down by CRISPR/Caspase-9 system, the proliferation and invasive ability of U251 increased remarkably. (portlandpress.com)
  • But when TET1 was overexpressed in U251 cells, the proliferation and invasion were impaired. (portlandpress.com)
  • Our experiments define STAT5 as a master regulator of NK-cell proliferation and lytic functions. (aacrjournals.org)
  • Interestingly, cells engineered to express more telomerase retained telomeres and the ability for extended proliferation. (encyclopedia.com)
  • p53-expressing senescent stellate cells release factors that skew macrophage polarization towards a tumor-inhibiting M1-state capable of attacking senescent cells in culture, whereas proliferating p53-deficient stellate cells secrete factors that stimulate polarization of macrophages into a tumor-promoting M2-state and enhance the proliferation of premalignant cells. (pubmedcentralcanada.ca)
  • Consequently, p53 mutations enable the unrestrained proliferation of damaged cells and thereby promote tumorigenesis in many tissue types. (pubmedcentralcanada.ca)
  • The epidermal growth factor receptor (EGFR) is widely expressed in the gastrointestinal tract and stimulates proliferation of a range of cell types, including epithelial cells [ 1 ]. (pubmedcentralcanada.ca)
  • Selective inhibition of COX-2 and PGE 2 production and influencing NO synthesis in the tumor microenvironment may represent important goals for prevention or therapy of colon cancers. (springer.com)
  • Furthermore, these results highlight the importance of pathologically relevant tissue culture models to study the complex physical and chemical processes by which the cancer microenvironment mediates new vessel formation. (sigmaaldrich.com)
  • Tumor microenvironment represents an attractive new drug target because it allows complex interaction between a tumor and its surrounding normal cells, molecules, and blood vessels, which all participate in tumor progression. (hindawi.com)
  • Hypoxia, glucose deprivation and acidosis are the hallmarks of tumor microenvironment. (hindawi.com)
  • This study investigated the upregulation of ABCG2 by these adverse growth conditions within the tumor microenvironment. (hindawi.com)
  • A better understanding of ABCG2 regulation by the tumor microenvironment may help design novel strategies to improve treatment outcome. (hindawi.com)
  • ABCG2 also plays a critical role in hypoxic defense mechanisms within the tumor microenvironment [ 3 ]. (hindawi.com)
  • They can be thought of as inhabiting within a complex milieu of normal cells, blood vessels, endogenous small molecules, and secreted factors, which collectively comprise the tumor microenvironment. (hindawi.com)
  • Recent advances have indicated that the tumor microenvironment is critically important for cancer initiation, progression, metastasis, and drug resistance, thus providing opportunities for therapeutic intervention. (hindawi.com)
  • This study aims to evaluate the regulation of ABCG2 in response to a few characteristic growth conditions within the tumor microenvironment, including hypoxia, glucose deprivation, and low pH. (hindawi.com)
  • The matched series of normal MCF-10A, pre-malignant MCF-10AT, and malignant MCF-10CA1a cells offers a unique opportunity to study the mechanisms of malignant progression both in a three-dimensional microenvironment and in the same cell background. (biomedsearch.com)
  • Furthermore, these spheroids allow the investigation of tumour-stroma interactions and a better reflection of in vivo conditions of cancer cells in their microenvironment. (nih.gov)
  • The result is a scaffold- and coating-free method for 3D cell culture that can be used for controlling the cellular architecture, as well as the cellular and molecular composition of the microenvironment in and around the formed cell structures. (diva-portal.org)
  • In 2016, Olivier Feron's UCLouvain team, which specialises in oncology, discovered that cells in an acidic microenvironment (acidosis) within tumours replace glucose with lipids as an energy source in order to multiply. (technologynetworks.com)
  • Prominent desmoplastic reaction involving cancer-associated fibroblasts (CAFs) and the immune cells in the tumor microenvironment (TME) and their cross-talk play a significant role in tumor immune escape and progression. (uni-wuerzburg.de)
  • To identify the key cellular mechanisms induce an immunosuppressive tumor microenvironment, we established 3D co-culture model with pancreatic cancer cells, Pancreatic cancer (PC) remains one of the most challenging solid tumors to treat with a high unmet medical need as patients poorly respond to standard-of-care-therapies. (uni-wuerzburg.de)
  • To identify the key cellular mechanisms induce an immunosuppressive tumor microenvironment, we established 3D co-culture model with pancreatic cancer cells, CAFs, monocyte as well as T cells. (uni-wuerzburg.de)
  • As one of the main regulatory components in the tumor microenvironment, tumor-associated macrophages (TAMs) are especially important in GBM, given their significant correlation with patient prognosis and glioma progression and grades 5 , 6 . (nature.com)
  • The importance of the microenvironment in breast cancer progression: recapitulation of mammary tumorigenesis using a unique human mammary epithelial cell model and a three-dimensional culture assay. (jbmethods.org)
  • Impact of the 3D microenvironment on phenotype, gene expression, and EGFR inhibition of colorectal cancer cell lines. (jbmethods.org)
  • Recent studies have shown that TMV contain numerous types of bioactive molecules and affect normal stromal cells in the tumor microenvironment. (plos.org)
  • These findings offer new insights into cancer therapies and the crosstalk between tumor and endothelial cells mediated by TMV in the tumor microenvironment. (plos.org)
  • HIF-1α critically regulates the interaction of neoplastic CLL cells with the leukemic microenvironment. (bloodjournal.org)
  • We then devised an assay to recapitulate in culture, within two weeks of incubation, critical aspects of the microenvironment in vivo including the typical tissue histology and stromal reaction. (jci.org)
  • p53 also promotes cellular senescence, a tumor suppressive program that involves stable cell cycle arrest and secretion of factors that modify the tissue microenvironment. (pubmedcentralcanada.ca)
  • Hence, p53 can act non-cell autonomously to suppress tumorigenesis by promoting an anti-tumor microenvironment, in part, through secreted factors that modulate macrophage function. (pubmedcentralcanada.ca)
  • Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells. (semanticscholar.org)
  • Treated prostate tumor cell lines trended toward apoptosis-induction, as evident in bcl-xl/bax ratios (scirp.org)
  • Figure 2: Induction of apoptosis in diffuse large B-cell lymphoma cell line U-2932 by etoposide treatment. (dsmz.de)
  • Western blot analysis was performed to study Boswellia sacra essential oil-regulated proteins involved in apoptosis, signaling pathways, and cell cycle regulation. (biomedcentral.com)
  • Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
  • The number of colonies in the clonogenic assay, the foci formation of RAD51 and γH2AX, and the induction of apoptosis were not affected by PTEN introduction in the HEC-6 PTEN + cells. (biomedcentral.com)
  • Double KO mice of Vegf and hypoxia inducible factor-1α ( Hif-1α ) showed increased life span and suppressed tumor growth due to increased apoptosis. (nature.com)
  • Unlike genes such as Myc , adenovirus E1A , and E2F-1, which, when overexpressed, activate the ARF-p53 pathway and trigger apoptosis, DMP1, like ARF itself, does not induce programmed cell death. (pnas.org)
  • Therefore, apart from its recently recognized role in protecting cells from potentially oncogenic signals, ARF can be induced in response to antiproliferative stimuli that do not obligatorily lead to apoptosis. (pnas.org)
  • In its role as a transcription factor, it activates a series of genes that can restrict cell cycle progression and trigger apoptosis. (pnas.org)
  • It encodes two distinct tumor suppressor proteins: p16 INK4a , which inhibits the phosphorylation of the retinoblastoma protein by cyclin D-dependent kinases ( 13 ), and p19 ARF ( 14 ), which stabilizes and activates p53 to promote either cell cycle arrest or apoptosis (reviewed in ref. 15 ). (pnas.org)
  • 11. The isolated stem cell of claim 8, wherein said polypeptide is tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). (freepatentsonline.com)
  • The mechanism studies showed that these siRNAs could activate the response of human tumor cells to exogenous nucleic acids, induce pyroptosis and apoptosis in the presence of GSDME, but induce apoptosis in the absence of GSDME. (frontiersin.org)
  • Independent of retinoblastoma and p53 tumor suppressor pathway alterations, flavopiridol induced apoptosis in all cell lines but through a caspase-independent mechanism. (aacrjournals.org)
  • Ectopic expression of wild-type S1PR2, but not a point mutant incapable of activating downstream signaling pathways, induces apoptosis in DLBCL cells and restricts tumor growth in subcutaneous and orthotopic models of the disease. (bloodjournal.org)
  • Targeting Notch and Hedgehog simultaneously induced apoptosis, decreased cell growth, and inhibited colony-forming ability more dramatically than monotherapy. (aacrjournals.org)
  • Cell viability, apoptosis, and related factors were determined using cell counting kit-8 (CCK-8), Annexin-V/PI (propidium) assay, enzyme-linked immunosorbnent assay (ELISA), RT-qPCR, and Western blot assays after miR-218 mimics has been transfected to HeLa cervical cancer cells. (springer.com)
  • MiR-218 overexpression was found to inhibit cell viability and promoted apoptosis via activating the expression of Cleaved-Caspase-3 and to inhibit the expression of Survivin, immune factors (TGF-β, VEGF, IL-6, PGE2, COX-2), and JAK2/STAT3 pathway. (springer.com)
  • The p53 tumor suppressor can restrict malignant transformation by triggering cell-autonomous programs of cell cycle arrest or apoptosis. (pubmedcentralcanada.ca)
  • Cis -diamminedichloroplatinum(II) (cisplatin)-induced renal proximal tubular apoptosis is known to be preceded by actin cytoskeleton reorganization, in conjunction with disruption of cell-matrix and cell-cell adhesion. (aspetjournals.org)
  • In the present study, we show that the proinflammatory cytokine tumor necrosis factor α (TNF- α ) aggravated these cisplatin-induced F-actin and cell adhesion changes, which was associated with enhanced cisplatin-induced apoptosis of immortalized proximal tubular epithelial cells. (aspetjournals.org)
  • Pharmacological inhibition of Rho kinase signaling re-established the synergistic apoptosis induced by combined cisplatin/TNF- α treatment of shRelB cells. (aspetjournals.org)
  • In conclusion, our study shows for the first time that RelB is required for the cisplatin/TNF- α -induced cytoskeletal reorganization and apoptosis in renal cells by controlling a Rho kinase-dependent signaling network. (aspetjournals.org)
  • Once delivered, granzymes are able to induce apoptosis and cause target cell death. (wikipedia.org)
  • When HeLa cells are exposed to sodium butyrate, a substance reported to suppress the malignant phenotype in several tumor cell lines, they lose their sensitivity to the cyanate metabolite. (aacrjournals.org)
  • Circulating tumour cells demonstrate an altered response to hypoxia and an aggressive phenotype. (nih.gov)
  • Hypoxia was further demonstrated to cause a more malignant anchorage-independent growth phenotype in the resistant cells, which can be abolished by knocking down ABCG2. (hindawi.com)
  • Increasingly transformed MCF-10A cells have a progressively tumor-like phenotype in three-dimensional basement membrane culture. (biomedsearch.com)
  • Furthermore, the fibroblast cell line showed an expression of α-SMA only in co-culture with the cancer cell line A549, thereby indicating a mesenchymal to mesenchymal shift to an even more myofibroblast phenotype. (nih.gov)
  • Recent experiments show that human colon carcinoma (HCT-8) cells display metastasis like phenotype (MLP) when they are cultured on 2D substrates having physiologically relevant stiffness (20-47 kPa), but not on very stiff (3.6 GPa) substrates 10-12 .These cells first form tumor-like cell clusters and then dissociate from one another, starting from the periphery. (jove.com)
  • Using this model, we analysed the influence of tumor cells and fibroblasts on monocytes and their immune suppressive phenotype. (uni-wuerzburg.de)
  • These co-culture spheroid polarized monocyte derived macrophages (MDMs) were poorly differentiated and had an M2 phenotype. (uni-wuerzburg.de)
  • Here, using a genetic targeting strategy, we investigated the contribution of Hif-1α to the changes of tumor phenotype in the Vegf -deleted islet tumors in RIP1-Tag2 mice. (nature.com)
  • Tumor cells were shown to specifically denude the venules both in culture and in vivo, explaining the VSMC phenotype in the stroma. (jci.org)
  • Myeloid‐derived suppressor cells (MDSCs) promote tumor progression. (semanticscholar.org)
  • She plans a study that will correlate type of fatty acid with tumor stage and grade in order to obtain a clearer picture of specific effects of different fats on tumor progression. (ucsf.edu)
  • Treatment refractory patients with metastatic cancer show increased numbers of circulating tumour cells (CTCs), which are also associated with disease progression. (nih.gov)
  • The interplay between glioblastoma stem cells (GSCs) and tumor-associated macrophages (TAMs) promotes progression of glioblastoma multiforme (GBM). (nature.com)
  • Abstract Background Many factors influence breast cancer progression, including the ability of progenitor cells to sustain or increase net tumour cell numbers. (lenus.ie)
  • Tumor angiogenesis is necessary for solid tumor progression and metastasis [1] . (plos.org)
  • Tumor blood vessels provide nutrition and oxygen, promote tumor progression, and serve as gatekeepers for tumor cells to metastasize to other organs. (plos.org)
  • Recent studies report that crosstalk between tumor cells and stromal cells are important for tumor progression or acceleration of tumor malignancy by secreting microvesicles (MVs) [11] , [12] . (plos.org)
  • Here, we demonstrated that the in vivo injection of recombinant human IL-15 (200 µg/kg) or murine IL-15 (3 µg/kg) to tumor-bearing NOD- SCID-IL2Rg−/− (NSI) mice resulted in increased tumor progression and CD45+ CD11b+ Gr-1+ CD215+ cell expansion in the tumors and spleen. (frontiersin.org)
  • These findings provide evidence that IL-15 may promote tumor cell progression via CD215+ myeloid cells, and IGF-1 may be an important candidate that IL-15 facilitates tumor growth. (frontiersin.org)
  • These findings suggest that ankyrin-Tiam1 interaction plays a pivotal role in regulating Rac1 signaling and cytoskeleton function required for oncogenic signaling and metastatic breast tumor cell progression. (rupress.org)
  • Scientists have also learned much about cancer initiation and progression through the use of cells in culture. (encyclopedia.com)
  • tumour hypoxia induces metastasis-associated genes. (nih.gov)
  • Poly-α2,8-sialic acid (PSA) has been implicated in numerous normal and pathological processes, including development, neuronal plasticity, and tumor metastasis. (sciencemag.org)
  • We demonstrated that VEGF-targeted drugs, as well as gene deletion of Vegf , inhibit primary tumor growth but promote tumor invasiveness and metastasis in the RIP1-Tag2 model 2 . (nature.com)
  • Gene-targeted and lymphocyte subset-depleted mice were used to establish the relative importance of natural killer (NK) and NK1.1 + T (NKT) cells in protection from tumor initiation and metastasis. (rupress.org)
  • In the models examined, CD3 − NK cells were responsible for tumor rejection and protection from metastasis in models where control of major histocompatibility complex class I-deficient tumors was independent of IL-12. (rupress.org)
  • Although it is likely that endogenously produced IL-12 may also stimulate NKT cells to produce IFN-γ and activate NKT cell cytolytic function, no study to date has evaluated the natural ability (i.e., not exogenously activated by IL-12 or α-GalCer) of NKT cells to reject tumor growth or metastasis. (rupress.org)
  • Both the positivity rate and the number of CTCs were significantly correlated with tumor size, portal vein tumor thrombus, differentiation status, and the disease extent as classified by the TNM (tumor-node-metastasis) classification and the Milan criteria. (aacrjournals.org)
  • Hepatocyte growth factor (HGF) is found in tumor microenvironments, and interaction with its tyrosine kinase receptor Met triggers cell invasion and metastasis. (biologists.org)
  • Histological diagnosis of metastatic stage IV or locally advanced (unresectable) stage III cancer of any solid tumor type. (knowcancer.com)
  • Circulating tumor cells (CTCs) are living solid-tumor cells that separate from either primary or secondary tumors and go on to move through the bloodstream. (oncologynurseadvisor.com)
  • Derivation of primary cell cultures from solid tumor material involves multiple critical handling steps. (miltenyibiotec.com)
  • Our results show that the method can be used for determining the collective ability of a given number of NK cells to defeat a solid tumor having a certain size, shape and composition. (diva-portal.org)
  • Preliminary studies on the control of differentiation in Class 1 clones suggest that a minimum cell density is required before differentiation takes place. (aacrjournals.org)
  • CONCLUSION: Here we report on the growth and differentiation properties of these three matched cell lines in three-dimensional basement membrane culture. (biomedsearch.com)
  • However, the patterns of gene expression in the HaCaT and A431 cells were different, and higher levels of involucrin synthesis were observed in A431 but not in HaCaT cells, suggesting that JA activated differentiation of the tumor A431 cells only. (springer.com)
  • Here, we demonstrate that MELK depletion by shRNA diminishes the growth of GSC-derived mouse intracranial tumors in vivo, induces glial fibrillary acidic protein (+) glial differentiation of GSCs leading to decreased malignancy of the resulting tumors, and prolongs survival periods of tumor-bearing mice. (wiley.com)
  • PRDM14 functions in embryonic stem cell (ESC) maintenance to promote the expression of pluripotency-associated genes while suppressing differentiation genes. (biologists.org)
  • Leukemia is preceded by a dramatic expansion of cells resembling hematopoietic stem cells and lymphoid-committed progenitors prior to disease onset, accompanied by a blockage in B-cell differentiation at the early pro-B stage. (biologists.org)
  • When confronted with tumor cells in this assay, fibroblasts readily converted into a graded pattern of myogenic differentiation, strongest in the immediate vicinity of tumor cells. (jci.org)
  • 5 The TGFβ family consists of three members (TGFβ1-3) which act as multifunctional proteins regulating growth and differentiation of various cell types. (bmj.com)
  • Its function is implicated in a receptor tyrosine kinase signaling pathway that specifies differentiation and terminal cell fate. (wikipedia.org)
  • Working with human prostate cancer cells in tissue culture, Hughes-Fulford and her fellow researchers identified for the first time a direct chain of causation: When introduced into prostate tumor cells in culture, omega-6 fatty acid causes the production of cPLA2, which then causes the production of the enzyme COX2. (ucsf.edu)
  • Oxygen status and tissue dimensionality are critical determinants of tumor angiogenesis, a hallmark of cancer and an enduring target for therapeutic intervention. (sigmaaldrich.com)
  • The production of vascular endothelial growth factor (VEGF) and other hypoxia-induced angiogenic cytokines to promote increase tissue vascularization, and the metabolic switch from oxidative to glycolytic metabolism represent the two major adaptive responses to enhance cell survival under tumor hypoxic condition. (hindawi.com)
  • Lung colony formation by elutriated B16a cells (i.e., cells freshly isolated from tumor tissue) was consistently inhibited by CGS14854, whereas inhibition of lung colony formation by cultured B16a cells was dependent upon culture conditions. (rti.org)
  • Objective: To collect high quality, representative tissue material from tumors and manage its distribution to different laboratories. (scirp.org)
  • To improve this complex procedure, we developed a workflow allowing for rapid and flexible tissue preparation for subsequent derivation of primary tumor cell cultures using our novel Pancreas TumorMACS™ Medium. (miltenyibiotec.com)
  • The gentleMACS™ Dissociator in combination with the Tumor Dissociation Kit, human ensures rapid and standardized dissociation of tissue samples into single-cell suspensions. (miltenyibiotec.com)
  • Single-cell suspensions from human melanoma tissue were prepared using the Tumor Dissociation Kit in combination with the gentleMACS™ Dissociator. (miltenyibiotec.com)
  • Finally, viability amounted to 86% at a yield of 5.8×10⁷ cells per gram of tissue. (miltenyibiotec.com)
  • Get more information on storage and dissociation of tumor tissue here. (miltenyibiotec.com)
  • Here, an optimized protocol is developed that describes the isolation of primary human colon cells from healthy and cancerous surgical human tissue samples. (jove.com)
  • For example, mammary epithelial cells (MECs) form normal acinar parenchyma when cultured on 150 Pa substrates that is similar to the stiffness of healthy mammary tissue. (jove.com)
  • The method gently arranges cells in a suspension into a single aggregate in each well of the microplate and, by this, nucleates 3D tissue-like cell growth for culture times between two and seven days. (diva-portal.org)
  • The ultrasound-based method itself is generic and can meet any demand from applications where it is advantageous to monitor cell culture from production to analysis of 3D tissue or tumor models using microscopy in one single microplate device. (diva-portal.org)
  • Global concordance for each culture/tissue pair was assayed evaluating the correlations between whole-genome CN profiles and SNP allelic calls. (biomedcentral.com)
  • A very good overlap between the CNAs of each culture and corresponding tissue was observed. (biomedcentral.com)
  • The finding, reinforced by high whole-genome CN correlations and SNP call concordances, provided evidence that each culture was derived from its corresponding tissue and maintained the genomic alterations of parental tumor. (biomedcentral.com)
  • These cells have been confirmed as maintaining the characteristics of the original tumor tissue through genomic, gene expression, and histological analyses. (essenbioscience.com)
  • Intriguingly, miRNA expression profiling has shown that miRNAs are globally down-regulated in tumors relative to normal tissue ( 10 ). (pnas.org)
  • Tissue microarray analysis with 91 HGG tumors demonstrates that the proportion of MELK (+) cells is a statistically significant indicator of postsurgical survival periods. (wiley.com)
  • As a tumor grows, the existing blood supply becomes inefficient at supporting the tissue, and areas of the tumor become hypoxic. (biomedcentral.com)
  • Methods Primary cultures were established from human breast tumour and adjacent non-tumour tissue. (lenus.ie)
  • ATCC has designed tumor cell panels based on the tissue of tumor origin, each annotated with details regarding known mutations - enabling more intelligent choices when selecting cell-based research models. (atcc.org)
  • Here, we reported that the expression of TET1 in glioma tissue was significantly lower than the corresponding non-tumour normal tissues, and the concentration of TET1 is negatively correlated with the glioma WHO classification. (portlandpress.com)
  • Elevated glutathione (GSH) levels have been detected in many tumors compared with the healthy, surrounding tissue. (aspetjournals.org)
  • Because quantitative and/or qualitative biochemical differences exist between tumors and the healthy surrounding tissue, it may be possible to selectively deliver drugs to the tumor by designing prodrugs that are metabolized more rapidly or in greater quantities in the tumor than in the surrounding tissue ( Dubowchik and Walker, 1999 ). (aspetjournals.org)
  • Recent evidence has assigned distinct features and functions to tissue-specific NK cells ( 2 ). (aacrjournals.org)
  • To put cells into culture, the tissue of interest is exposed to enzymes that dissociate the tissue to release the component cells. (encyclopedia.com)
  • The general process of cell culture has been used extensively since the early 1900s for research on tissue growth and development, virus biology, properties of cancer cells, studies relating to aging, genetics, and gene therapy. (encyclopedia.com)
  • British biologist who developed ways to grow cells outside the body ("tissue culture") in order to more closely study the cells and the effects of hormones, vitamins, and other chemicals. (encyclopedia.com)
  • In the 1960s, biologists found that normal human fibroblasts, cells derived from connective tissue , had a predictable limit in their ability to proliferate in culture. (encyclopedia.com)
  • RESULTS: We found that the expression of both connective tissue growth factor (CTGF/CCN2) and type I collagen was negatively regulated in CCD-1068SK fibroblast cells under direct co-culture conditions. (uct.ac.za)
  • In this tissue, tumor initiation is often preceded by hepatic fibrosis resulting from liver damage produced by chronic hepatitis, alcohol or chemical insults. (pubmedcentralcanada.ca)
  • The EGFR is also frequently overexpressed in human colorectal tumors when compared with normal intestinal tissue, and this is associated with increased metastatic potential and poor prognosis [ 5-7 ]. (pubmedcentralcanada.ca)
  • However, only a few studies have reported a prominent role for NK cells on the growth of primary tumors in mice without using biological response modifiers to magnify the response ( 2 )( 3 ). (rupress.org)
  • Analysis of ten primary tumors implicated converted fibroblasts (10/10), vascular smooth muscle cells (4/10), and pericytes (1/10) in the stromal reaction. (jci.org)
  • How these factors and the Met signaling pathway influence primary tumors to regulate gene expression, angiogenesis, proteinase secretion, and cell motility and invasion are major areas of ongoing research. (biologists.org)
  • Glucose depletion is often observed in malignant tumors due to insufficient blood supply in the core of solid tumors [ 5 ] and increased rate of glycolysis [ 6 ]. (hindawi.com)
  • The pre-malignant MCF-10AT cells and malignant MCF-10CA1a lines were sequentially derived from the MCF-10A parental cell line first by expression of a constitutively active T24 H-Ras generating the MCF-10AT cell line. (biomedsearch.com)
  • HCT-8 cells cultured on very hard polystyrene substrates do not exhibit these malignant traits. (jove.com)
  • The DN:DP ratio was also higher in malignant MDA-MB-231 cells relative to non-tumourogenic MCF-10A cells. (lenus.ie)
  • They represent the first line of defense against infected, stressed, and malignant cells. (aacrjournals.org)
  • Multiple developmental pathways including Notch, Hedgehog, and Wnt are active in malignant brain tumors such as medulloblastoma and glioblastoma (GBM). (aacrjournals.org)
  • Glioblastoma (GBM) is the most common malignant primary central nervous system tumor in adults and is characterized by resistance to chemo- and radiotherapy ( 1 ). (aacrjournals.org)
  • Furthermore, if the cells were subcultivated extensively, they acquired malignant properties characteristic of cancer cells. (encyclopedia.com)
  • Radiation therapy plays a critical role in the local and regional control of malignant tumors. (aspetjournals.org)
  • However, a rapid inhibition of protein synthesis in cultured tumor cells can be achieved by reaction of cyanate with the drug-metabolizing system of liver microsomes. (aacrjournals.org)
  • We first investigated the effects of cell source and culture conditions on lung colony formation by i.v. injected B16a (B16 amelanotic melanoma) cells and inhibition of tumor colony formation by the thromboxane A2 synthase inhibitor, CGS14854. (rti.org)
  • However, the inhibition of TNF production could not be explained by the inhibitory effect of IL-6 on cell growth, nor was it due to a reduction in cell viability. (jimmunol.org)
  • An inhibition of TNF production by IL-6 was also demonstrated in cultured human peripheral blood monocytes. (jimmunol.org)
  • Therefore, PARP inhibition might be useful for various types of tumors with HR defects, independent of the BRCA status (BRCAness). (biomedcentral.com)
  • Stimulation of lipolysis in cultured fat cells by tumor necrosis factor, interleukin-1, and the interferons is blocked by inhibition of prostaglandin synthesis. (ugent.be)
  • FEINGOLD K, DOERRLER W, DINARELLO C, Fiers W, GRUNFELD C. Stimulation of lipolysis in cultured fat cells by tumor necrosis factor, interleukin-1, and the interferons is blocked by inhibition of prostaglandin synthesis. (ugent.be)
  • Therefore, inhibition of tumor angiogenesis is a promising strategy for the treatment of cancer. (plos.org)
  • Maximal blastogenic lymphocyte responses were induced by 5 X 10 4 irradiated tumor cells, and inhibition of blastogenesis was induced by 10 6 tumor cells. (elsevier.com)
  • The inhibition of blastogenesis produced by higher doses of tumor cells was caused by a nondialyzable factor released into the supernatant of cultured, irradiated, or mitomycin-treated cells. (elsevier.com)
  • Inhibition of the proteasome by TA in Jurkat cells results in accumulation of two natural proteasome substrates, the cyclin-dependent kinase inhibitor p27 Kip1 and the proapoptotic protein Bax, followed by growth arrest in G 1 and induction of apoptotic cell death. (aacrjournals.org)
  • Moreover, miR-17 inhibition led to tumour growth suppression and up-regulation of RND3 expression in a nude mouse xenograft model. (portlandpress.com)
  • Notch may directly suppress Hedgehog via Hes1 mediated inhibition of Gli1 transcription, and targeting both pathways simultaneously may be more effective at eliminating GBMs cells. (aacrjournals.org)
  • PRDM14-induced leukemic cells contain high levels of activated NOTCH1 and downstream NOTCH1 targets, including MYC and HES1, and are sensitive to pharmacological inhibition of NOTCH1 with the γ-secretase inhibitor DAPT. (biologists.org)
  • In two of the three cell lines, TGFβ-1 treatment resulted in transactivation of a TGFβ responsive reporter construct as well as inhibition of c-myc and induction of p21 (WAF1) expression. (bmj.com)
  • Neuroendocrine tumour cells of the gastroenteropancreatic tract are subject to paracrine and autocrine growth inhibition by TGFβ-1, which may account in part for the low proliferative index of this tumour entity. (bmj.com)
  • Deletion of oncogenic KRAS not only resensitized tumor cells to EGFR inhibition but also promoted EGF-induced NRAS activation, ERK and AKT phosphorylation, and c-FOS transcription. (pubmedcentralcanada.ca)
  • The poor responsiveness of mutant KRAS tumor cells to EGFR inhibition and activation was accompanied by a reduced capacity of these cells to bind and internalize EGF and by a failure to retain EGFR at the plasma membrane. (pubmedcentralcanada.ca)
  • This caused an EGFR-dependent increase in basal and EGF-stimulated ERK phosphorylation but failed to restore tumor cell sensitivity to EGFR inhibition. (pubmedcentralcanada.ca)
  • HGF-induced NHE activity via increased net acid production, and inhibition of NHE activity with 5-( N -ethyl- N -isopropyl)-amiloride (EIPA), or a combination of the NHE1-specific drug cariporide and the NHE3-specific drug s3226 prevented HGF-induced anterograde trafficking and induced retrograde trafficking in HGF-overexpressing cells. (biologists.org)
  • Expression of the DMP1 transcription factor, a cyclin D-binding Myb-like protein, induces growth arrest in mouse embryo fibroblast strains but is devoid of antiproliferative activity in primary diploid fibroblasts that lack the ARF tumor suppressor gene. (pnas.org)
  • RND3 expression was found to be significantly lower in CRC tissues than in normal tissues and adenomas, indicating that RND3 may act as a tumour suppressor gene in CRC. (portlandpress.com)
  • Most colorectal tumors are initiated by inactivating mutations in the tumor suppressor gene APC [ 2 ]. (pubmedcentralcanada.ca)
  • Nonetheless, it is suspected to act as a tumor suppressor gene. (wikipedia.org)
  • E and F) Differences between CTCs and parental MDA-MB-231 cells with respect to induction of HIF1α target genes. (nih.gov)
  • The deregulation of coding and non-coding genes due to genomic and epigenomic alterations or aberrant signal transduction is a common feature of tumor cells. (dsmz.de)
  • We analyzed expression of genes associated with the activation of ER stress ( GRP78 , ATF4 , CHOP ), the structure of the ER and Golgi complex, and synthetic processes in the HaCaT and A431 cell lines. (springer.com)
  • JA induced expression of genes responsible for the activation of ER stress and caused hypertrophic changes in the Golgi complex in both cell lines. (springer.com)
  • In T-VEC, deletions of wild-type γ 34.5 and α 47 viral genes promote targeting tumor cells over nonneoplastic cells and enhance the body's natural antitumor response ( 7 ). (asm.org)
  • The recognition of this molecular heterogeneity might improve the selection of patients for targeted therapies and allow the identification of specific oncogenes and tumor suppressor genes to be used as novel clinical markers or therapeutic targets. (biomedcentral.com)
  • The carcinogenesis of CRC involves multiple alterations of oncogenes and tumour suppressor genes, such as inactivating APC (adenomatous polyposis coli) and TP53 (tumour protein 53) and oncogenically mutating Ras and BRAF [ 4 ]. (portlandpress.com)
  • Human breast cancer cells (MDA-MB-231) and anaplastic thyroid cancer cells (CAL62) were transduced with lentiviral particles, to express the Renilla luciferase and mCherry (mCherry-Rluc) reporter genes. (medsci.org)
  • We used GBM neurosphere lines to investigate the effects of a gamma-secretase inhibitor (MRK-003) on tumor growth, and chromatin immunoprecipitation to study the regulation of other genes by Notch targets. (aacrjournals.org)
  • Another difficulty with some cultured cells is their tendency to change their morphology , functions, or the range of genes they express. (encyclopedia.com)
  • Cancer cells were grown on collagen/Matrigel and embedded with or without stromal cells (hTERT-immortalized human PS-1 stellate cells or MRC-5 fibroblasts) for 7 days. (nih.gov)
  • We describe a novel 3D co-culture model using non-small cell lung cancer (NSCLC) cell lines in combination with lung fibroblasts. (nih.gov)
  • In these hanging drops the two NSCLC cell lines A549 and Colo699 were cultivated either alone or co-cultured with lung fibroblasts. (nih.gov)
  • 3D co-culture of monocytes with pancreatic cancer cells and fibroblasts induced the production of immunosuppressive cytokines which are known to promote polarization of M2 like macrophages and myeloid derived suppressive cells (MDSCs). (uni-wuerzburg.de)
  • Recapitulation of tumor environment in culture unravels diversity and implicates converted fibroblasts and recruited smooth muscle cells. (jci.org)
  • The aim of this study was to investigate mechanisms involved in tumour cell-mediated regulation of extracellular matrix and adhesion molecules in co-cultured fibroblasts. (uct.ac.za)
  • Further analysis revealed that Smad7, a known negative regulator of the Smad signalling pathway involved in CCN2 promoter regulation, was increased in directly co-cultured fibroblasts. (uct.ac.za)
  • ERK signalling was also shown to be impaired in CCD-1068SK fibroblasts after direct co-culture with MDA-MB-231 tumour cells, with Smad7 overexpression in fibroblasts leading to a similar decrease in ERK activity. (uct.ac.za)
  • These effects were not, however, seen in fibroblasts that were indirectly co-cultured with tumour cells. (uct.ac.za)
  • CONCLUSION: We therefore conclude that breast cancer cells require close contact with fibroblasts in order to upregulate Smad7 which, in turn, leads to decreased ERK signalling resulting in diminished expression of the stromal proteins CCN2 and type I collagen. (uct.ac.za)
  • HGF can be produced by both cancer-associated fibroblasts and tumor cells in a paracrine or autocrine manner, respectively. (biologists.org)
  • Prolonged culture resulted in a 10-fold decrease in the incidence of B16a lung colonies, whereas passage in vivo for 150 days did not affect lung colony formation by tumor cells isolated from enzymatic dispersates by centrifugal elutriation. (rti.org)
  • The results were validated and confirmed in vivo, with QNZ and papaverine exhibiting superior antitumor activity in a tumor xenograft model when combined with the VEGF inhibitor bevacizumab (avastin). (mdpi.com)
  • This model allows the investigation of tumour-stroma interactions and addresses the importance of having a more in vivo like cell culture model. (nih.gov)
  • Recently, the scientific revolution has improved cell culturing techniques by applying three dimensional (3D) culture system, which provides a similar physiologically relevant in-vivo model for studying various diseases including cancer. (eurekaselect.com)
  • In particular, cancer cells exhibiting in-vivo behavior in a model of 3D cell culture is a more accurate cell culture model to test the effectiveness of anticancer drugs or characterization of cancer cells in comparison with two dimensional (2D) monolayer. (eurekaselect.com)
  • Antoni D, Burckel H. Josset, Noel G. Three-dimensional cell culture: a breakthrough in-vivo. (eurekaselect.com)
  • They also significantly inhibited the growth of human tumor cells in vivo . (frontiersin.org)
  • These results demonstrated that TEC maintain distinct biological differences from NEC as observed in tumor blood vessels in vivo. (plos.org)
  • NK cells mediate spontaneous cytotoxicity against MHC class I-deficient tumor cells and their metastases ( 1 ), and they have long been known to participate in the innate immune response against transformed cells in vivo ( 1 )( 2 ). (rupress.org)
  • The in vivo antitumor activity of α-GalCer strongly resembles the antitumor activity mediated by IL-12 ( 9 )( 32 ), and indeed α-GalCer mediates its effects by inducing IL-12 production by dendritic cells (DCs) and IL-12 receptor and IFN-γ expression by NKT cells ( 33 ). (rupress.org)
  • A nticancer drug efficacy has been tested on circulating tumor cells ( CTC s) derived from patient blood samples after ex vivo expansion into CTC clusters. (nus.edu.sg)
  • In this study, we confirmed the migration of MSCs to tumor sites in cancer xenograft models using both in vivo and ex vivo BLI imaging. (medsci.org)
  • A protocol is described to extract primary human cells from surgical colon tumor and normal tissues. (jove.com)
  • Here, we explored whether ccRCC primary cultures, established from surgical tumor specimens, maintain the DNA profile of parental tumor tissues allowing a more confident CNAs and LOH discrimination with respect to the original tissues. (biomedcentral.com)
  • Using the Affymetrix SNP array technology, we performed the genome-wide copy number (CN) profiling of both cultures and corresponding tumor tissues. (biomedcentral.com)
  • These cultures showed a greater cell homogeneity and enrichment in tumor component than original tissues, thus enabling a better discrimination of CNAs and LOH. (biomedcentral.com)
  • differently, in original tissues the intensity of these deletions was weaken by normal cell contamination and LOH calls were missed. (biomedcentral.com)
  • In the case of cancer cells, tissues are collected from surgical specimens, biopsy specimens, ascitic fluid, or pleural fluid originating from patients who have consented to cooperate with the university hospital. (essenbioscience.com)
  • F-PDOs have been established from tumor tissues. (essenbioscience.com)
  • NKT cells are present in most tissues where T cells are found, in particular the liver, bone marrow, spleen, and thymus ( 14 )( 15 )( 16 ). (rupress.org)
  • The expression of miR-218 was negatively correlated with IDO1 in cervical cancer tissues and cells. (springer.com)
  • Media, supplements and accessory reagents are available for primary neurons and neural stem/progenitor cells from human, mouse and rat tissues. (stemcell.com)
  • Isolate, expand, and differentiate mouse and rat neural stem and progenitor cells from normal and tumor tissues. (stemcell.com)
  • Future improvements in the therapeutic index for radiotherapy depend on increasing the sensitivity of tumor cells to radiation and reducing the effects of radiation on normal tissues. (aspetjournals.org)
  • Summing up, we showed that addition of L-arginine at doses which did not stimulate NO level caused a significant decrease in COX-2 and PGE 2 amounts in co-cultures of colon tumor spheroids with normal epithelial cells and myofibroblasts. (springer.com)
  • BACKGROUND: MCF-10A cells are near diploid and normal human mammary epithelial cells. (biomedsearch.com)
  • As this epithelial to rounded morphological (E to R transition) change occurs, they proliferate, reduce cell-cell and cell-ECM adhesion, and become migratory. (jove.com)
  • Putative progenitor cell populations were isolated based on co-expression or concomitant absence of the epithelial and myoepithelial markers EPCAM and CALLA respectively. (lenus.ie)
  • Epithelial cell adhesion molecule (EpCAM) is an epithelial cell-specific adhesion molecule that is widely expressed on the surface of epithelial cells and epithelial-derived tumor cells ( 6 ). (aacrjournals.org)
  • Although HCC cells are epithelial cells, only about 35% of HCC cases express EpCAM ( 8, 9 ). (aacrjournals.org)
  • Aberrant Notch signaling was implicated in the initiation of T-cell lymphoblastic leukemia in the early 1990s ( 7 ), and has since been demonstrated in many different hematopoietic and epithelial tumors ( 8-10 ). (aacrjournals.org)
  • In the presence of chronic liver damage, we show that ablation of a p53-dependent senescence program in hepatic stellate cells increases liver fibrosis and cirrhosis associated with reduced survival, and enhances the transformation of adjacent epithelial cells into hepatocellular carcinoma. (pubmedcentralcanada.ca)
  • We now demonstrate that HGF induced the trafficking of lysosomes to the cell periphery, independent of HGF-induced epithelial-mesenchymal transition. (biologists.org)
  • This protective effect was associated with increased stress fiber formation, cell-matrix, and cell-cell adhesion in the shRNARelB (shRelB) cells during cisplatin/TNF- α treatment, mimicking an epithelial-to-mesenchymal phenotypic switch. (aspetjournals.org)
  • The proximal tubular epithelial cells (PTECs) are the primary target. (aspetjournals.org)
  • Recently a novel mechanism, by which some genetically deregulated and aggressive tumour cells generate "micro-vascular" channels without the participation of endothelial cells and independent of angiogenesis, has been proposed. (biomedsearch.com)
  • This has been termed "vasculogenic mimicry" and has implications beyond angiogenesis and adds another layer of complexity to the current concept for the generation of tumour micro-circulation. (biomedsearch.com)
  • 3D culture broadly regulates tumor cell hypoxia response and angiogenesis via pro-inflammatory pathways. (sigmaaldrich.com)
  • Taken together, our data suggest that pro-inflammatory pathways are critical regulators of tumor hypoxia response within 3D environments that ultimately impact tumor angiogenesis, potentially providing important therapeutic targets. (sigmaaldrich.com)
  • Novel cancer treatment strategies take advantage of tumor-induced vascularisation by combining standard chemotherapeutic agents with angiogenesis-inhibiting agents. (biomedcentral.com)
  • We postulated that the expression levels of angiogenesis-related proteins from various primary tumor cultures would be greater under hypoxic conditions than under normoxia. (biomedcentral.com)
  • We for the first time showed that endocytosis of TMV contributes to tumor angiogenesis. (plos.org)
  • Tumor blood vessels have been recognized as an important target for cancer therapy since Folkman introduced the concept that tumor growth is dependent on angiogenesis. (plos.org)
  • These findings reveal that STAT5-directed therapies may have negative effects: In addition to impairing NK-cell-mediated tumor surveillance, they may even promote tumor growth by enhancing angiogenesis. (aacrjournals.org)
  • We show that the new signal interceptors targeting the JAK-STAT5 pathway may have dangerous side effects that must be taken into account in clinical trials: inhibiting JAK-STAT5 has the potential to promote tumor growth by enhancing NK-cell-mediated angiogenesis. (aacrjournals.org)
  • IL-6 inhibits lipopolysaccharide-induced tumor necrosis factor production in cultured human monocytes, U937 cells, and in mice. (jimmunol.org)
  • This was followed by repeated selection for increasingly aggressive tumor formation from cells recovered from xenograft tumors in immuno-compromised mice, generating the MCF-10CA1a cell line. (biomedsearch.com)
  • When inoculated subcutaneously into the flanks of immuno-compromised mice, MCF-10AT cells occasionally form tumors, whereas MCF-10CA1a cells invariably form tumors with a shorter latency than MCF-10AT derived tumors. (biomedsearch.com)
  • The result: tumour development was significantly slowed compared to that in mice on a conventional diet. (technologynetworks.com)
  • At the same time, we examined xenografts of ACC2 in SCID mice and compared the nature of pseudocystic spaces formed in the transplants with that in the collagen gel cultures. (nii.ac.jp)
  • Transplants of ACC2 cells in SCID mice grew to form tumor messes in which pseudocysts were formed. (nii.ac.jp)
  • Publications] Munakata, R.: 'Pseudocyst tormation by adenoid cystic carcinoma cells collagen gel culture and in SCID mice' J Oral Pathol Med. (nii.ac.jp)
  • The increased invasiveness of tumors in VKO mice was diminished in DKO mice to the levels of wild-type mice. (nature.com)
  • Hif-1α deletion in the tumors of RIP1-Tag2/RipCre/Hif-1α fl/fl (RIP1-Tag2/HKO) mice was confirmed at the levels of genome, mRNA and protein (see Supplementary Fig. S1 ). (nature.com)
  • In B16F10-bearing C57BL/6 mice model, we found that murine IL-15 has antitumoral effect since the activation and expansion of CD8+ T cells with murine IL-15 treatment. (frontiersin.org)
  • But no enhanced or reduced tumor growth was observed in mice when human IL-15 was used. (frontiersin.org)
  • In particular, T cell receptor Jα281 gene-targeted mice confirmed a critical function for NKT cells in protection from spontaneous tumors initiated by the chemical carcinogen, methylcholanthrene. (rupress.org)
  • Transfer of purified polyclonal T cells from FBL (Friend MuLV-induced erythroleukemia cell line) vaccinated mice in naive animals can protect these mice against subsequent tumor challenge. (rupress.org)
  • FBL cells do not express MHC class II molecules, but CD4 + T cells can protect mice even in the absence of CD8 + T cells. (rupress.org)
  • These mice were rendered tolerant for env-specific Th responses and it was not possible to protect these mice against FBL tumors by vaccination. (rupress.org)
  • In addition, proteasome inhibitors have been shown to suppress human tumor growth in nude mice. (aacrjournals.org)
  • We show that STAT5 normally represses the transcription of VEGFA in NK cells, in both mice and humans. (aacrjournals.org)
  • When R26PR is mated to either of two Cre lines, Mx1-cre or MMTV-cre, mice develop early-onset T-cell acute lymphoblastic leukemia (T-ALL) with median overall survival of 41 and 64 days for R26PR;Mx1-cre and R26PR;MMTV-cre, respectively. (biologists.org)
  • The study also identifies a potential new molecular target for anti-tumor drugs: an enzyme known as cPLA2, which plays a key role in the chain leading from omega-6 fatty acids to prostate tumor cell growth. (ucsf.edu)
  • Prospective Randomized Comparative Study of Cell Transfer Therapy Using CD8+-Enriched Short-Term Cultured Anti-Tumor Autologous Lymphocytes Following a Non-Myeloablative Lymphocyte Depleting Chemotherapy Regimen Compared to High-Dose Aldesleukin in M. (knowcancer.com)
  • We examined the anti-tumor activity of olaparib, a PARP inhibitor, and its correlation between the sensitivity and status of PTEN in endometrial cancer cell lines. (biomedcentral.com)
  • These cells become immunogenic, lose their tumorigenicity, and even induce protection against wild-type MHC class II negative tumors, indicating that direct MHC class II presentation of tumor expressed antigens can induce efficient anti-tumor responses. (rupress.org)
  • ABCG2 promoter luciferase reporter gene assay showing that the promoter region harboring the HIF-1α response element is required for the activation of ABCG2 in HCT‐116 human colon cancer cell line. (hindawi.com)
  • The response to olaparib was evaluated using a clonogenic assay with SF50 values (concentration to inhibit cell survival to 50%) in 16 endometrial cancer cell lines. (biomedcentral.com)
  • The effects of PTEN on the sensitivity to olaparib and ionizing radiation (IR) exposure were compared between parental HEC-6 (PTEN-null) and HEC-6 PTEN + (stably expressing wild-type PTEN) cells by clonogenic assay, foci formation of RAD51 and γH2AX, and induction of cleaved PARP. (biomedcentral.com)
  • Fluorescent microbeads are embedded in soft gels near the cell culture surface, and traction assay is performed to assess cellular contractile stresses using free open access software. (jove.com)
  • Interlaboratory studies with the chinese hamster v79 cell metabolic cooperation assay to detect tumor-promoting agents. (cdc.gov)
  • The authors conclude that several characteristics of the assay may limit its usefulness for screening broad classes of chemicals for potential activity as tumor promoters. (cdc.gov)
  • The establishment of this assay and clarification of the origin of these cells pave the way for further analysis of the mechanisms of conversion, and of the consequence of such heterogeneity for diagnosis and treatment. (jci.org)
  • It is also believed to be a survival factor for cancer stem cells to drive tumor growth. (hindawi.com)
  • It may also contribute to the maintenance of stem or progenitor cells as a survival factor, ultimately driving tumor growth [ 4 ]. (hindawi.com)
  • Accordingly, tumor cells depend on cellular pathways promoting survival under such conditions. (mdpi.com)
  • Following failure of initial chemotherapy and radiation, effective palliation was achieved by using a sequence of electron-beam radiotherapy, low dose gemcitabine, and etoposide, resulting in significant periods of tumor regression and prolonged survival. (scirp.org)
  • Despite our reports of the objective regressions in patients receiving adoptive cell therapy (ACT), doubts have been raised concerning the possible influence of patient selection bias that may have accounted for the increase in survival using ACT compared to historical controls. (knowcancer.com)
  • The combined treatment reduced the survival of the resistant cells but in the gefitinib sensitive cells, the combined treatment increased survival, which was highly unexpected. (eurekaselect.com)
  • A limited number of astatine decays per cell gave low survival, which was expected. (eurekaselect.com)
  • Eke I, Cordes N. Radiobiology goes 3D: how ECM and cell morphology impact on cell survival after irradiation. (jbmethods.org)
  • Interleukin 15 (IL-15) regulates the development, survival, and functions of multiple innate and adaptive immune cells and plays a dual role in promoting both tumor cell growth and antitumor immunity. (frontiersin.org)
  • We have combined chromatin immunoprecipitation and gene expression profiling after FOXP1 depletion with functional screening to identify targets of FOXP1 contributing to tumor cell survival. (bloodjournal.org)
  • The forkhead box protein 1 (FOXP1) transcription factor is aberrantly expressed in the activated B-cell-like (ABC) subtype of diffuse large B-cell lymphoma (DLBCL) and represents a widely accepted biomarker for survival prognostication in DLBCL. (bloodjournal.org)
  • These findings demonstrate that Notch-targeted therapeutics can lead to alterations in other developmental signaling cascades that promote tumor survival, and suggest that combined treatment with Hedgehog pathway inhibitors may be able to increase the efficacy of GSIs in some cancer patients. (aacrjournals.org)
  • The antioxidant compound N -acetylcysteine blocked the curcumin-induced increased reactive oxygen species (ROS), sustained activation of ERK1/2, and decreased survival after IR in HeLa cells, implicating a ROS-dependent mechanism for curcumin radiosensitivity. (aspetjournals.org)
  • Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. (semanticscholar.org)
  • Accumulated evidence suggests that glioma stem cells (GSCs) may contribute to therapy resistance in high-grade glioma (HGG). (wiley.com)
  • Protein and phosphoprotein levels in glioma and adenocarcinoma cell lines grown in normoxia and hypoxia in monolayer and three-dimensional cultures. (jbmethods.org)
  • Increasing evidence suggests that the major obstacle to effective treatment of gliomas is the highly proliferative, migrative and invasive nature of glioma cells [ 4 ]. (portlandpress.com)
  • Mitochondrial damage measured by cytochrome c release and transmission electron microscopy was not observed in drug-treated glioma cells. (aacrjournals.org)
  • In glioma cell lines, overexpression of MDM2 has been associated with drug resistance ( 8 , 9 ). (aacrjournals.org)
  • In this study, we wished to examine the effect of flavopiridol on a panel of glioma cell lines, which have alterations in both the Rb and p53 tumor suppressor pathways. (aacrjournals.org)
  • COX2 has been implicated in the growth of many types of tumors," she notes. (ucsf.edu)
  • Metastatic HNSCC cells lines were found to have vasculogenic properties similar to HUVEC cell lines when compared to cell lines from their corresponding primary tumour. (biomedsearch.com)
  • The present study was undertaken to investigate the factors involved in determining the metastatic potential of cultured cells derived from solid tumors. (rti.org)
  • Metastatic Merkel Cell carcinoma (MCC) is a highly unusual and aggressive skin cancer that presents as a small, pink to violet skin lesion and metastasizes early in its growth. (scirp.org)
  • Metastatic MCC is generally treated with small cell lung cancer chemotherapy regimens, because the tumor consists of neuroendocrine cells, but patients generally do not have durable responses. (scirp.org)
  • Although this adoptive cell therapy (ACT) has been shown capable of mediating objective clinical responses in patients with metastatic melanoma, including patients who have previously been treated with aldesleukin or chemotherapy, the treatment is only available in the Surgery Branch, National Cancer Institute (NCI) and in just one or two other institutions in the United States. (knowcancer.com)
  • 1. Measurable metastatic melanoma with at least one lesion that is resectable for tumor infiltrating lymphocytes (TIL) generation. (knowcancer.com)
  • Thus it has been hypothesized that HCT-8 cells become metastatic due to their exposure to appropriate micro-environment. (jove.com)
  • It is generally accepted that the host immune status, particularly natural immune responses, is essential in controlling the dissemination and growth of metastatic tumors. (rupress.org)
  • The question of how this molecule is regulated in invasive and metastatic processes of breast cancer cells is addressed in the present study. (rupress.org)
  • The development of metastatic foci, resulting from an invasive primary tumor, is the leading cause of cancer-associated deaths. (biologists.org)
  • In xenograft tumor models, CD215+ myeloid cells, but not CD215 − cells, responded to human IL-15 stimulation and promoted tumor growth. (frontiersin.org)
  • Pediatric brain tumor cell lines. (semanticscholar.org)
  • We investigated whether brain tumors show resistance to therapies against Notch, and whether targeting multiple pathways simultaneously would kill brain tumor cells more effectively than monotherapy. (aacrjournals.org)
  • Culture and expand human neural stem cells and brain tumor stem cells in a serum-free and/or xeno-free system. (stemcell.com)
  • Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)
  • Poly (ADP-ribose) polymerase (PARP) plays key roles in the repair of DNA single-strand breaks, and a PARP inhibitor induces synthetic lethality in cancer cells with HR deficiency. (biomedcentral.com)
  • Therefore, selective inhibitors of COX-2 may also block PGE 2 generation minimizing its colorectal-tumor-promoting effects. (springer.com)
  • Specifically, we searched for inhibitors that selectively killed tumor cells growing in glucose-free but not in normal medium. (mdpi.com)
  • Given our findings, we propose that compounds targeting metabolically challenged tumors, such as inhibitors of mitochondrial activity, be considered as a therapeutic strategy in cancer. (mdpi.com)
  • Culture dimensionality influences the resistance of glioblastoma stem-like cells to multikinase inhibitors. (jbmethods.org)
  • However, even the use of targeted therapies can be limited by the emergence of resistant tumor cells, and resistance to EGFR inhibitors ( 5 ) and Hedgehog inhibitors ( 6 ) has already been documented. (aacrjournals.org)
  • Here, we show that deletion of the oncogenic KRAS allele from colorectal tumor cells resensitizes those cells to EGFR inhibitors. (pubmedcentralcanada.ca)
  • Our results demonstrate a causal role for oncogenic KRAS in desensitizing tumor cells not only to EGFR inhibitors but also to EGF itself. (pubmedcentralcanada.ca)
  • However, it is unclear how these microenvironmental conditions interact to promote neovascularization, due in part to a lack of comprehensive, unbiased data sets describing tumor cell gene expression as a function of oxygen levels within three-dimensional (3D) culture. (sigmaaldrich.com)
  • Microarray gene expression analysis of tumor cells cultured in 2D versus 3D under ambient or hypoxic conditions revealed striking interdependence between culture dimensionality and hypoxia response, which was mediated in part by pro-inflammatory signaling pathways. (sigmaaldrich.com)
  • Cell lysis for Real Time PCR and quantification of gene expression of CX 43, 32 and E-cadherin in canine mast cells. (scirp.org)
  • For gene expression quantification of CX 43, 32 and E-cadherin in canine mast cell tumors (MCT), 95.5% of samples were considered to be of good quality. (scirp.org)
  • DMP1 binds to a single canonical recognition site in the ARF promoter to activate gene expression, and in turn, p19 ARF synthesis causes p53-dependent cell cycle arrest. (pnas.org)
  • These clonal lines may provide a suitable model system for the study of gonadotropin actions and regulation of the expression of differentiated functions of Leydig cells. (nih.gov)
  • Xenografts generated from CTCs and parental cells were compared.Asparagine synthetase (ASNS) expression was also higher in CTCs.Xenografts generated from CTCs were larger and heavier, and metastasised faster than MDA-MB-231 xenografts. (nih.gov)
  • B) Expression of the anoxia factor ATF3 in CTCs and MDA-MB-231 cells. (nih.gov)
  • D) Expression of ATF4 and its target gene ASNS in CTCs, compared with parental MDA-MB-231 cells. (nih.gov)
  • We report that cell surface PSA expression can be reversibly inhibited by a small molecule, N -butanoylmannosamine (ManBut). (sciencemag.org)
  • Cells are labeled green by GFP expression. (dsmz.de)
  • Proliferative Activity of Canine Mast Cell Tumours Evaluated by Bromodeoxyuridine Incorporation and Ki-67 Expression," Journal of Comparative Pathology, Vol. 127, No. 4, 2002, pp. 233-238. (scirp.org)
  • PS-1 cells were confirmed as stellate based on the expression of key cytoskeletal proteins and lipid vesicles. (nih.gov)
  • 0.01) in the presence of stromal cells with predominant cytoplasmic expression. (nih.gov)
  • Ki67 expression varied significantly between mono- and co-cultures in both tumour cell lines. (nih.gov)
  • In addition, the cell models exhibit predictable growth rates and stable proteomic expression. (technologynetworks.com)
  • RAD51 expression was observed broadly and was not associated with PTEN status in the 16 cell lines. (biomedcentral.com)
  • The expression level of nuclear PTEN was not elevated within 24 h following IR in the HEC-6-PTEN + cells. (biomedcentral.com)
  • Phenotypic characterization of the monocytes after 3D co-culture with tumor/fibroblast spheroids was performed by analysing the expression of defined cell surface markers and soluble factors. (uni-wuerzburg.de)
  • In particular, the let-7 miRNA family has been proposed to function in tumor suppression, because reduced expression of let-7 family members is common in non-small cell lung cancer (NSCLC). (pnas.org)
  • Ectopic expression of let-7g in K-Ras G12D -expressing murine lung cancer cells induced both cell cycle arrest and cell death. (pnas.org)
  • Ectopic expression of K-Ras G12D largely rescued let-7g mediated tumor suppression, whereas ectopic expression of HMGA2 was less effective. (pnas.org)
  • Finally, in an autochthonous model of NSCLC in the mouse, let-7g expression substantially reduced lung tumor burden. (pnas.org)
  • and assessing tumor tropic potential of the stem cell based upon said expression level of CXCR4, said affinity for the chemokine SDF-1, or both. (freepatentsonline.com)
  • Following the down-expression of TET1, the level of autophagy in U251 cells decreased accordingly.However, when TET1 was overexpressed in U251 cells, the level of autophagy incraesed. (portlandpress.com)
  • Given that MDM2 protein can confer oncogenic properties under certain circumstances, loss of MDM2 expression in tumor cells could promote increased chemosensitivity. (aacrjournals.org)
  • 1-3 Reactive oxygen species (ROS) may serve as a common intracellular messenger for various redox-sensitive transcription pathways that lead to adhesion molecule expression in vascular endothelial cells. (ahajournals.org)
  • NK cells have organ-specific properties, such as distinct profiles of receptor expression or cytokine production ( 3 ). (aacrjournals.org)
  • Aberrant expression of the oncogenic transcription factor forkhead box protein 1 (FOXP1) is a common feature of diffuse large B-cell lymphoma (DLBCL). (bloodjournal.org)
  • 7 Therefore, aberrant FOXP1 expression is a common feature of various types of mature B-cell lymphomas that can result from either genetic abnormalities or transcriptional or posttranscriptional dysregulation. (bloodjournal.org)
  • however, aberrant expression is associated with tumor initiation in a wide variety of human cancers, including breast cancer and leukemia. (biologists.org)
  • Expression of TGFβ-1 and its receptors, Smads and Smad regulated proteins, was examined in surgically resected NET specimens and human NET cell lines by immunohistochemistry, reverse transcriptase-polymerase chain reaction, immunoblotting, and ELISA. (bmj.com)
  • Conversely, high expression of EGFR ligands predicts response to anti-EGFR therapy but only in the subset of wild-type KRAS tumors [ 15,16 ]. (pubmedcentralcanada.ca)
  • Adequate T helper cell activation is essential in the initiation of an immune reaction. (rupress.org)
  • Although tumor cells can directly present endogenously processed antigenic peptide in surface MHC class I molecules to CD8 + CTL precursors, initiation of tumor-specific CTL responses is likely to involve indirect presentation of tumor antigens by specialized APCs. (rupress.org)
  • Here, we utilized alginate-based, oxygen-controlled 3D tumor models to study the interdependence of culture context and the hypoxia response. (sigmaaldrich.com)
  • The CTCs and parental MDA-MB-231 cells were incubated at 21 and 0.2% (hypoxia) oxygen, respectively. (nih.gov)
  • Parental MDA-MB-231 cells induced ATF3 in hypoxia, whereas CTCs expressed it constitutively. (nih.gov)
  • Hypoxia induced ATF4 and the HIF1alpha target gene apelin in CTCs, but not in parental cells. (nih.gov)
  • Hypoxia induced lower levels of carbonic anhydrase IX (CAIX), GLUT1 and BCL2/adenovirus E1B 19-KD protein-interacting protein 3 (BNIP3) proteins in CTCs than in parental cells, supporting an altered hypoxia response. (nih.gov)
  • In chronic hypoxia, CTCs demonstrated greater colony formation than parental cells. (nih.gov)
  • Circulating tumour cells (CTCs), isolated and cultured from murine blood, demonstrate a distinct response to hypoxia compared with parental MDA-MB-231cells. (nih.gov)
  • To determine whether CTCs have an altered hypoxia response, they had to be isolated and grown in culture. (nih.gov)
  • Reporter activity in HCT-116 cells transiently transfected with the various ABCG2 promoter constructs was measured with or without 24-h pretreatment with 2-DG (20 mM) or hypoxia. (hindawi.com)
  • Importantly, glucose deprivation and hypoxia were also found to enhance the resistance level of ABCG2-overexpressing resistant cells with pre-existing genetic and epigenetic MDR mechanisms. (hindawi.com)
  • Cancer cells are often confronted with a remarkable reduction in oxygen supply inside solid tumors, leading to intratumoral hypoxia. (hindawi.com)
  • For VEGF, the target of current therapies, this correlation between hypoxia and higher cytokine levels was greater in primary breast and lung carcinoma cells than in ovarian carcinoma cells or tumor cell lines. (biomedcentral.com)
  • Hypoxia-inducible transcription factors (HIFs) regulate a wide array of adaptive responses to hypoxia and are often activated in solid tumors and hematologic malignancies due to intratumoral hypoxia and emerging new layers of regulation. (bloodjournal.org)
  • Mechanistic studies indicated that LD/LP tumor cells induced aggregation of homologous platelets, whereas HD/HP B16a cells failed to induce significant platelet aggregation. (rti.org)
  • Here, we found that siRNAs targeting mouse-specific lncRNA AA388235 could significantly induce death of human tumor cells, although it has no effect on mouse tumor cells and normal human cells. (frontiersin.org)
  • CD133(+) and CD133(-) glioblastoma-derived cancer stem cells show differential growth characteristics and molecular profiles. (semanticscholar.org)
  • In this study, we aimed to investigate the associations between COX-2, PGE 2 and NO in co-cultures of human colon cancer spheroids obtained from different tumor grades with normal human colonic epithelium and myofibroblast monolayers. (springer.com)
  • Researchers based at the Massachusetts General Hospital (MGH) Center for Engineering in Medicine and the MGH Cancer Center have created a microchip device, called the CTC-iChip, which can capture circulating tumor cells in order to track ongoing genetic mutation within a tumor. (oncologynurseadvisor.com)
  • Circulating tumor cells captured with a microchip-based device developed at the Massachusetts General Hospital (MGH) Center for Engineering in Medicine and the MGH Cancer Center can be cultured to establish cell lines for genetic analysis and drug testing. (oncologynurseadvisor.com)
  • ABCG2 is an efflux transporter commonly found to overexpress in multidrug resistant (MDR) cancer cells. (hindawi.com)
  • Cancer cells do not exist in isolation. (hindawi.com)
  • The group of Dr. Sonja Eberth focuses on transcriptional deregulation in cancer and its functional impact on the biology of cancer cells, especially in variants of blood cancer, namely leukemia and lymphoma. (dsmz.de)
  • Vlad-Fiegen A, Freytag NV, Dorn S, Müller O, Eberth S. The Wnt pathway target gene CCND1 changes mitochondrial localization and decreases mitochondrial activity in colorectal cancer cell line SW480. (dsmz.de)
  • 1 Centre for Tumour Biology, Institute of Cancer, Barts and the London School of Medicine and Dentistry, Charterhouse Square, London EC1M 6BQ. (nih.gov)
  • Presence of either stromal cell type translocated Ezrin from apical (when stromal cells were absent) to basal aspects of cancer cells, where it was associated with invasive activity. (nih.gov)
  • The presence of red blood cells (RBCs) hampers the accurate determination of cell viability as well as absolute cell counts for tumor-infiltrating leukocytes, cancer stem cells, and other tumor-resident cells. (miltenyibiotec.com)
  • AMSBIO has introduced a new range of cancer cell line models and culture media for the most difficult to culture tumor types where no models may exist. (technologynetworks.com)
  • Unlike traditional protocols for cell line creation, these cancer models eliminate the possibility of large scale cellular adaptation through culture and genetic drift. (technologynetworks.com)
  • Beneficially the cancer cell models are stable and show a high level of agreement to the original tumor genotype through 150 passages via SNP analysis. (technologynetworks.com)
  • The quality control of the cancer cell models includes screened for all major categories of cell contaminants, including mycoplasma, microbial contaminants, viruses, cell line cross-contamination and STR profiling. (technologynetworks.com)
  • The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
  • Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
  • All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
  • Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
  • Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
  • Our results suggest that olaparib, a PARP inhibitor, is effective on certain endometrial cancer cell lines. (biomedcentral.com)
  • IMPORTANCE Cancer cells are exposed to HSV-1 during oncolytic virotherapy with the intention of killing tumor cells. (asm.org)
  • Cancer cells respond to matrix mechanical stiffness in a complex manner using a coordinated, hierarchical mechano-chemical system composed of adhesion receptors and associated signal transduction membrane proteins, the cytoskeletal architecture, and molecular motors 1, 2 . (jove.com)
  • It is worth noting that these experiments are carried out with immortalized cancer cell lines or murine derived primary cells, not with primary human cancer cells. (jove.com)
  • Thanks to the support of the Fondation Louvain, the Belgian Cancer Foundation and the Télévie telethon, the team quickly identified that these acidotic tumour cells responded in diametrically opposite ways depending on the fatty acid they were absorbing. (technologynetworks.com)
  • Brain tumors are the leading cause of cancer-related death in children but high-grade gliomas in children and adolescents have remained a relatively under-investigated disease despite this. (semanticscholar.org)
  • All-trans retinoic acid modulates cancer stem cells of glioblastoma multiforme in an MAPK-dependent manner. (semanticscholar.org)
  • Pancreatic cancer (PC) remains one of the most challenging solid tumors to treat with a high unmet medical need as patients poorly respond to standard-of-care-therapies. (uni-wuerzburg.de)
  • Ishiguro T, Ohata H, Sato A, Yamawaki K, Enomoto T, Okamoto K. Tumor‐derived spheroids: relevance to cancer stem cells and clinical applications. (eurekaselect.com)
  • At the division of Cell Factory, cells are used to establish new evaluation systems for anti-cancer drugs, and the efficacy of anti-cancer drugs is evaluated. (essenbioscience.com)
  • In contrast to general cultured cancer cells, it is possible to use them for evaluations of anti-cancer drugs that reflect clinical use. (essenbioscience.com)
  • Importantly, let-7g-mediated tumor suppression was more potent in lung cancer cell lines harboring oncogenic K-Ras mutations than in lines with other mutations. (pnas.org)
  • The singularly most frequently disrupted gene in cancer is p53, whose loss of function occurs in more than half of human tumors ( 1 ). (pnas.org)
  • INK4a / ARF is perhaps the second most commonly disrupted locus in cancer cells ( 12 ). (pnas.org)
  • Not surprisingly, human cancer cells that retain p53 function overexpress Mdm2 ( 25 ) or sustain deletions that dismantle ARF function ( 12 ). (pnas.org)
  • T-cell therapies are not just for cancer. (the-scientist.com)
  • Transcriptome profiling in clinical breast cancer: from 3D culture models to prognostic signatures. (jbmethods.org)
  • Martin KJ, Patrick DR, Bissell MJ, Fournier M V. Prognostic breast cancer signature identified from 3D culture model accurately predicts clinical outcome across independent datasets. (jbmethods.org)
  • ATCC Tumor Cell Panels combine authenticated, well-characterized cell lines with mutation data from the Sanger Institute Catalogue of Somatic Mutations in Cancer (COSMIC) to create powerful tools for cancer research and drug discovery. (atcc.org)
  • Manni, A & Wright, C 1983, ' Effect of tamoxifen and α-difluoromethylornithine on clones of nitrosomethylurea-induced rat mammary tumor cells grown in soft agar culture ', Cancer Research , vol. 43, no. 3, pp. 1084-1086. (elsevier.com)
  • BIGHEART Acting Director Lim Chwee Teck and team have developed a unique approach for growing patient's cancer cells in a microfluidic device that enables doctors to test a range of drugs on tumour cell clusters to determine efficacy, and develop personalised therapies. (nus.edu.sg)
  • Because only the residual cancer cells outside the liver of a recipient can cause recurrence after liver transplantation in HCC patients, isolated tumor cells must be the source of recurrence ( 4 ). (aacrjournals.org)
  • MSCs are able to deliver drugs to target cells, and are an ideal candidate for cancer therapy. (medsci.org)
  • Two of the many areas of scientific study where uses of cell culture techniques have had major impact are human aging and cancer research. (encyclopedia.com)
  • Cancer cell lines, which can grow indefinitely in culture, also retain long telomeres. (encyclopedia.com)
  • Ground-breaking immune therapy promises to deliver vital evidence in the fight against cancer as researchers from the Centre for Cancer Biology open a new clinical trial using genetically engineered immune cells to treat solid cancers. (news-medical.net)
  • The phase 1 clinical trial will test the feasibility and safety of CAR-T cells - genetically modified white blood cells harvested from a patient's own blood with the unique ability to directly attack and kill cancers - to treat advanced solid tumors including small cell lung cancer, sarcomas and triple negative breast cancer. (news-medical.net)
  • The research scientist in charge of manufacturing the CAR-T cell product and following the patients' responses to treatment is UniSA's Dr Tessa Gargett, a Cancer Council Beat Cancer Project Early Career Fellow from the Centre for Cancer Biology. (news-medical.net)
  • Chimeric antigen receptor (CAR) T cells are a promising new technology in the field of cancer immunotherapy. (news-medical.net)
  • The research forms part of the 'CARPETS' phase 1 clinical trial initiated by Professor Michael Brown, Director of the Cancer Clinical Trials Unit at the RAH to treat advanced melanoma patients, now extended to include patients with other advanced solid tumors including, small cell lung cancer, sarcomas and triple negative breast cancer. (news-medical.net)
  • As indoleamine-2,3-dioxygenase 1 (IDO1) is critical in tumor immune escape, we determined to study the regulatory mechanism of miR-218 on IDO1 in cervical cancer. (springer.com)
  • MiR-218 inhibits immune escape of cervical cancer cells by direct downregulating IDO1. (springer.com)
  • See commentary " Tumor Suppression: p53 Alters Immune Surveillance to Restrain Liver Cancer " in Curr Biol , volume 10 on page R527. (pubmedcentralcanada.ca)
  • In tumor xenografts, we observed significant growth reduction of both murine and human non-small cell lung tumors when overexpression of let-7g was induced from lentiviral vectors. (pnas.org)
  • We now report that STAT5-deficient NK cells can be rescued by overexpression of BCL2. (aacrjournals.org)
  • Of 16 human colorectal tumors with activating mutations in KRAS , 15 displayed loss of basolateral EGFR localization. (pubmedcentralcanada.ca)
  • For instance, it is possible that colorectal tumors with KRAS mutations preferentially develop in an (epi)genetic background of EGFR independence. (pubmedcentralcanada.ca)
  • INTRODUCTION: Vascuologenesis is the de novo establishment of blood vessels and vascular networks from mesoderm-derived endothelial cell precursors (angioblasts). (biomedsearch.com)
  • The endothelial growth factor antibodies used did not inhibit or stimulate cell growth when compared to control but did discourage vascular mimicry. (biomedsearch.com)
  • Its existence also suggests a further process that of the development of tumour mosaic vessels as the neo-vasculature integrates with the existing endothelial lined systems. (biomedsearch.com)
  • Recombinant human tumor necrosis factor alpha promotes adherence of Staphylococcus aureus to cultured human endothelial cells. (asm.org)
  • The ability of TNF-alpha to augment staphylococcal adherence to endothelial cells was contingent upon the presence of plasma factors. (asm.org)
  • Thus, the complex interaction among cytokines (such as TNF), plasma factor(s), and the endothelium serves to modulate bacterial adherence to endothelial cells. (asm.org)
  • Tumor necrosis factor-mediated release of platelet-derived growth factor from cultured endothelial cells. (rupress.org)
  • In the present study, we found that the macrophage-derived polypeptide, tumor necrosis factor (TNF), releases a factor from human umbilical vein endothelial cells (EC) that is mitogenic for SMC. (rupress.org)
  • Such a marked suppression by TNF-α was not observed in confluent bovine aortic endothelial cells and human fibroblastic IMR-90 cells. (elsevier.com)
  • Increasing evidence indicates that tumor endothelial cells (TEC) differ from normal endothelial cells (NEC). (plos.org)
  • Furthermore, there are many differences at the molecular and functional levels between tumor endothelial cells (TEC) and normal EC (NEC) [3] , [4] . (plos.org)
  • Objective- We tested the hypothesis that carvedilol, a β-adrenoceptor and α-adrenoceptor antagonist with potent antioxidant property, could inhibit tumor necrosis factor-α (TNF-α)-induced endothelial adhesiveness to human mononuclear cells (MNCs), an early sign of atherogenesis. (ahajournals.org)
  • Compared with control condition, pretreatment of carvedilol (10 μmol/L for 18 hours) or probucol (5 μmol/L for 18 hours), but not propanolol, prazosin, or both propanolol and prazosin significantly decreased TNF-α-stimulated adhesiveness of cultured human aortic endothelial cells (HAECs) to MNCs. (ahajournals.org)
  • In the early phase of atherogenesis, cell surface adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1), intercellular cell adhesion molecule-1 (ICAM-1), and endothelial cell selectin (E-selectin) could express on endothelial cells to recruit circulating mononuclear cells (MNCs), mainly monocytes, and facilitate their binding to endothelium and migrating to subendothelial space. (ahajournals.org)
  • RESULTS: There were observable differences in growth of the cells on laboratory plastic and collagen matrix. (biomedsearch.com)
  • A study conducted at the San Francisco VA Medical Center (SFVAMC) has demonstrated that omega-6 fatty acids such as the fat found in corn oil promote the growth of prostate tumor cells in the laboratory. (ucsf.edu)
  • The study was led by Millie Hughes-Fulford, PhD, director of the Laboratory of Cell Growth at SFVAMC and scientific advisor to the U.S. Undersecretary of Health for the Department of Veterans Affairs. (ucsf.edu)
  • In turn, COX2 stimulates the release of PGE2, a hormone-like molecule that promotes cell growth. (ucsf.edu)
  • Hughes-Fulford also found that flurbiprofen, a non-steroidal anti-inflammatory drug commonly prescribed for arthritis, blocked the production of cPLA2 and broke the chain leading to cell growth. (ucsf.edu)
  • So if you can find a way to block that cascade in the tumor, starting with cPLA2, you might have a new way of modifying or slowing tumor growth. (ucsf.edu)
  • These drugs have been shown to be effective against tumor growth as well as in treating the pain associated with inflammatory conditions such as arthritis, but have been implicated in increased risk of cardiovascular problems in people who take them regularly. (ucsf.edu)
  • The density of beta AR, assayed by 125I-pindolol binding, was increased two- to threefold by a 24-h incubation of the cells with IL-1 alpha, IL-1 beta, and TNF-alpha (EC50: 2.7, 8.2, and 24 pM, respectively), although a series of other cytokines and growth factors did not have this effect. (jimmunol.org)
  • AMSBIO's extensive quality control process ensures stable growth rate for each lot of Cellaria cell models delivered. (technologynetworks.com)
  • 3D melanoma cultures were established by placing tumor cells on the surface of a Matrigel matrix, which was followed by the growth of tumor cells on the matrix surface and invasion of the Matrigel matrix by some tumor cells to form multicellular tumor spheroids within the matrix. (asm.org)
  • However, HSV-1 infection did not lead to a complete destruction of tumor cells in the 3D cultures during a 17-day observation period and, surprisingly, HSV-1 infection promoted the growth of some melanoma cells within the matrix as determined by the significantly increased size of residual viable multicellular tumor spheroids in virus-inoculated 3D cultures at 17 days after virus inoculation. (asm.org)
  • These findings suggest that although HSV-1 oncolytic virotherapy may cause extensive tumor cell killing, it may also be associated with the unintended promotion of the growth of some tumor cells. (asm.org)
  • Our observations reported here suggest that potential dangers of HSV-1 oncolytic therapy include promotion of growth of some tumor cells. (asm.org)
  • Furthermore, our findings raise the possibility that HSV-1 infection of neoplastic cells during natural infections or vaccinations may promote the growth of tumors. (asm.org)
  • Our study indicates that HSV-1 infection of 3D tumor cell cultures provides an experimental platform in which mechanisms of HSV-1-mediated promotion of tumor cell growth can be effectively studied. (asm.org)
  • Platelet-derived growth factor (PDGF) is a 30,000-Mr glycoprotein that is chemotactic and mitogenic for vascular smooth muscle cells (SMC). (rupress.org)
  • Interaction of tumor promoters and growth factors with cultured cells. (elsevier.com)
  • A localized hypoxic environment occurs during tumor growth necessitating an angiogenic response or tumor necrosis results. (biomedcentral.com)
  • Three-dimensional cell growth confers radioresistance by chromatin density modification. (jbmethods.org)
  • Furthermore, we found that human IL-15 mediated insulin-like growth factor-1 production in CD215+ myeloid cells and blocking IGF-1 reduced the tumor-promoting effect of IL-15. (frontiersin.org)
  • These data indicate that the soft-agar culture technique can be successfully used to investigate the endocrine mechanisms affecting the growth of individual experimental mammary cancers. (elsevier.com)
  • The data also suggest an important role of polyamines in mediating the growth of this mammary tumor model. (elsevier.com)
  • We recently developed a simple but unique microfluidics-based culture approach that requires minimal preprocessing (~30 min) and does not require prior enrichment of CTC s or depend on the use of growth factor supplements. (nus.edu.sg)
  • Cell culture describes the laboratory growth of cells derived from plants or animals. (encyclopedia.com)
  • Dispersed cells are then transferred to a suitable growth medium and allowed to attach to the surface of culture flasks. (encyclopedia.com)
  • Cell culture requires careful attention to the growth medium to ensure cells are given all the components they require to grow. (encyclopedia.com)
  • Often the culture medium requires growth factors or hormones to stimulate growth. (encyclopedia.com)
  • Media used to propagate cells are rich in nutrients and, therefore, support growth of a multitude of organisms. (encyclopedia.com)
  • The ability to culture cells allowed the laboratory growth of polio virus to produce vaccines that nearly eliminated polio as a disease. (encyclopedia.com)
  • Ongoing genomic instability in cells such as CICs can subsequently allow for outgrowth of unique subclones, which acquire additional driver mutations that endow them with a selective growth advantage ( Hanahan and Weinberg, 2011 ). (biologists.org)
  • BACKGROUND: Recent studies have revealed that interactions between tumour cells and the surrounding stroma play an important role in facilitating tumour growth and invasion. (uct.ac.za)
  • The tumor cells secrete immunosuppressive factors, to name a few, transforming growth factor-β(TGF-β). (springer.com)
  • The role of transforming growth factor β-1 (TGFβ-1) in neuroendocrine tumour biology is currently unknown. (bmj.com)
  • Growth regulation was evaluated by cell numbers, soft agar assays, and cell cycle analysis using flow cytometry. (bmj.com)
  • TGFβ-1 inhibited anchorage dependent and independent growth in a time and dose dependent manner in TGFβ-1 responsive cell lines. (bmj.com)
  • It therefore appears likely that growth factors or cytokines may be involved in growth regulation of NET cells. (bmj.com)
  • In this context, several growth factors and cytokines are secreted by NET cells, including transforming growth factor β (TGFβ). (bmj.com)
  • However, both murine and human IL-15 promote CD45+ CD11b+ Gr-1+ CD215+ cells expansion. (frontiersin.org)
  • In this study, possible correlations of these properties to the uptake, retention and photosensitization activity of these oxazines were examined in a human bladder tumor cell line (MGH. (spie.org)
  • The main effector cells responsible for tumor eradication were identified as CD8 + cytotoxic T cells that were found to recognize a recently described immunodominant viral gag-encoded cytotoxic T lymphocyte (CTL) epitope, which is unrelated to the viral env-encoded T helper peptide sequence. (rupress.org)
  • Sodium cyanate, a selective inhibitor of protein synthesis in animal tumors in situ , has no comparable effect when added to tumor cells in culture. (aacrjournals.org)
  • L-arginine (2 mM), a substrate for nitric oxide synthases (NOS), decreased COX-2 and PGE 2 levels, while N G -nitro-L-arginine methyl ester (L-NAME) (2 mM), a NOS inhibitor, had no influence on COX-2 and PGE 2 levels but limited tumor cell motility. (springer.com)
  • NS398 (75 μM), a selective COX-2 inhibitor, had no significant influence on NO level but decreased motility of tumor cells. (springer.com)
  • The level of nitrite and nitrate in serum or culture supernatants can be significantly reduced by application of L-NAME, a competitive inhibitor of NOS. (springer.com)
  • Eke I, Leonhardt F, Storch K, Hehlgans S, Cordes N. The small molecule inhibitor QLT0267 Radiosensitizes squamous cell carcinoma cells of the head and neck. (jbmethods.org)
  • Five of six cell lines have deletions of the CDK inhibitor p16 INK4a (all except CCF-STTG1) inactivating the Rb cell cycle control pathway. (aacrjournals.org)
  • Transfecting CRC cells with an inhibitor of miR-17 lowered their ability to proliferate and induced G 0 /G 1 arrest. (portlandpress.com)
  • We also evaluated the effect of combined therapy with a Hedgehog inhibitor (cyclopamine) in GBM and medulloblastoma lines, and in primary human GBM cultures. (aacrjournals.org)
  • We are using state-of-the-art molecular and cell biology techniques like RNA immunoprecipitation (Figure 1) and next-generation sequencing as well as diverse cell based assays including life-cell imaging (Figure 2/Movie). (dsmz.de)
  • Briefly, granular proteins were differentially extracted from GRC.014.22 and GRC.014.24, eosinophilic cell lines established in our laboratory from a patient with moderate asthma. (scirp.org)
  • Basic proteins and polyamino acids are taken up by mammalian cells at rates up to 3000 times greater than serum albumin. (sciencemag.org)
  • The infected human cells expressed, upon cultivation with dexamethasone, MMTV structural proteins and released spiked B-type virions, the infectivity of which could be neutralized by anti-MMTV antibody. (biomedcentral.com)
  • The cognate human and mouse proteins are 95% identical, and hDMP1 on human chromosome 7q21 is frequently deleted in myeloid leukemia, connoting a possible role for DMP1 as a tumor suppressor ( 28 ). (pnas.org)
  • Tumor-derived MVs (TMV) contain bioactive molecules such as microRNAs (miRNA), mRNAs, and/or proteins inside themselves [15] . (plos.org)
  • As of early 2016, there are three families belonging to the MACPF superfamily: 1.C.12 - The Thiol-activated Cholesterol-dependent Cytolysin (CDC) Family 1.C.39 - The Membrane Attack Complex/Perforin (MACPF) Family 1.C.97 - The Pleurotolysin Pore-forming (Pleurotolysin) Family Proteins containing MACPF domains play key roles in vertebrate immunity, embryonic development, and neural-cell migration. (wikipedia.org)
  • The MACPF domain is structurally similar to pore-forming cholesterol-dependent cytolysins from gram-positive bacteria, suggesting that MACPF proteins create pores and disrupt cell membranes similar to cytolysin. (wikipedia.org)
  • It provides a feasible mechanism of early tumour vascular supply which can co-exist and incorporate with later angiogenic mechanisms. (biomedsearch.com)
  • The results indicate that multiple effects of IL-1 in a cultured tumor cell line involve different mechanisms, suggesting heterogeneity of IL-1R and/or coupling of IL-1R to distinct, nuclear, and nonnuclear, effector pathways. (jimmunol.org)
  • However, the detailed molecular mechanisms underlying the relationship between these two cell types remain unclear. (nature.com)
  • Procedures are described for the induction, preparation, and testing of an active cytochrome P-450 fraction which converts cyanate to a short-lived dialyzable metabolite that selectively inhibits amino acid incorporation in tumor cells without a corresponding effect on protein synthesis in normal cells. (aacrjournals.org)
  • Here, we show that let-7 functionally inhibits non-small cell tumor development. (pnas.org)
  • Here, we report that indeed TA potently and specifically inhibits the chymotrypsin-like activity of purified 20S proteasome (IC 50 = 0.06 μg/ml), 26S proteasome of Jurkat T-cell extracts, and 26S proteasome of living Jurkat cells. (aacrjournals.org)
  • Our present study suggests that TA targets and inhibits the proteasome in tumor cells, which may contribute to the previously observed anticarcinogenic activity of TA. (aacrjournals.org)
  • Here, we report that ester-bond containing TA potently and selectively inhibits the chymotrypsin-like activity in purified 20S proteasome, 26S proteasome of Jurkat T-cell extracts, and 26S proteasome in intact Jurkat cells. (aacrjournals.org)
  • Cotransfection of SP1 cells with green fluorescent protein (GFP)-tagged Tiam1 fragment cDNA and Tiam1 cDNA effectively blocks Tiam1-ankyrin colocalization in the cell membrane, and inhibits GDP/GTP exchange on Rac1 by ankyrin-associated Tiam1 and tumor-specific phenotypes. (rupress.org)
  • Additionally, LD/LP cells demonstrated higher adhesion to endothelium than HD/HP B16a cells. (rti.org)
  • These changes of adhesion molecules were not observed in the primary culture of the tumor cells under hypoxic conditions. (nature.com)
  • Here, we present an easy-to-perform, reliable and time-efficient method for the production of 3D cell lysates without compromising cell adhesion before cell lysis. (jbmethods.org)
  • Burridge K. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. (jbmethods.org)
  • These formulations generally comprise hybridoma of at least one antigen presenting cell fused to either at least one tumor cell or at least one virally infected cell, or the products of co-cultures of antigen presenting cells and either tumor cells or virally infected cells. (freepatentsonline.com)
  • wherein said first cells are antigen presenting cells selected from the group consisting of macrophages, B-cells and dendritic cells, and said second cells are selected from the group consisting of tumor cells and virally infected cells. (freepatentsonline.com)
  • J. J. Abadie, M. A. Amardeilh and M. E. Delverdier, "Immunohistochemical Detection of Proliferating Cell Nuclear Antigen and Ki-67 in Mast Cell Tumors from Dogs," Journal of the American Animal Hospital Association, Vol. 215, No. 11, 1999, pp. 1629-1634. (scirp.org)
  • The RIP1-Tag2 transgenic mouse model overexpresses SV40 T antigen under the control of the insulin promoter, resulting in development of islet cell tumors 4 . (nature.com)
  • The peptide-specific CD4 + T cells did not directly recognize the tumor cells, indicating involvement of cross-priming by tumor-associated antigen-presenting cells. (rupress.org)
  • Protection is dependent on tumor-specific CTLs in this model system and requires cross-priming of tumor antigens by specialized antigen-presenting cells. (rupress.org)
  • The ability to effectively culture CTCs and examine them for genetic changes can be used to find targetable mutations and discover which therapeutic drugs are most effective. (oncologynurseadvisor.com)
  • The present invention relates to tumor tropic stem cells, and particularly to neural stem cells, and their use as delivery vehicles for therapeutic gene products to neoplastic foci. (freepatentsonline.com)
  • 6. The isolated stem cell of claim 5, wherein said heterologous gene encodes a polypeptide of therapeutic use in the treatment of a disease condition. (freepatentsonline.com)
  • Our study not only demonstrates that the siRNAs designed for knocking down mouse-specific lncRNA AA388235 can be potential tumor therapeutic drugs, but also suggests that non-human species-specific lncRNAs are a huge potential library that can be used to design siRNAs for tumor treatment. (frontiersin.org)
  • As hematogenous spread is the major route of HCC recurrence ( 5 ), detection of circulating tumor cells (CTC) undoubtedly has important clinical significance in predicting recurrence and monitoring therapeutic efficiency in HCC patients. (aacrjournals.org)
  • Mesenchymal stem cells (MSCs) show therapeutic effects in various types of diseases. (medsci.org)
  • they may also serve as a potential type of therapeutic cells. (medsci.org)
  • The emergence of therapeutic resistance is a significant concern when targeting many signaling pathways and tumor types. (aacrjournals.org)
  • Oncolytic HSV-1 therapy is dependent upon virus replication in tumor cells and is augmented by host antiviral and infection-induced antitumor immune responses ( 1 , 3 - 6 ). (asm.org)
  • Factors that may limit the effectiveness of HSV-1 oncolytic therapy include restricted intratumoral spread of oncolytic virus, activation of intracellular tumor defenses that limit virus-induced tumor cell killing, and quick virus clearance by the host immune system ( 1 , 8 - 12 ). (asm.org)
  • 8. The isolated stem cell of claim 6, wherein said polypeptide is involved in an immune response. (freepatentsonline.com)
  • MVs have recently attracted attention among researchers of immune response and embryo development, playing a role in cell-to-cell communication [13] . (plos.org)
  • Many prior studies have suggested the importance of interleukin 15 (IL-15) as a regulator for immune cells ( 3 - 6 ). (frontiersin.org)
  • Natural tumor surveillance capabilities of the host were investigated in six different mouse tumor models where endogenous interleukin (IL)-12 does or does not dictate the efficiency of the innate immune response. (rupress.org)
  • These unusual regulatory cells that bridge the innate and acquired immune systems recognize CD1d-bound glycolipid ligands such as α-galactosylceramides (α-GalCer [( 11 ), ( 12 )]) and glycosylphosphatidylinositols ( 13 ). (rupress.org)
  • The new clinical trial will allow researchers to learn more about how CAR-T cells interact with solid tumors in the hope that this form of immune-based therapy may one day treat a wide range of different cancers. (news-medical.net)
  • Essentially, CAR-T cells are super-powered immune cells which work by enlisting and strengthening the power of a patient's immune system to attack tumors. (news-medical.net)
  • We suggest that "vasculogenic mimicry" maybe a common neoplastic phenomena which appears to also be dictated by the cells micro-environment. (biomedsearch.com)
  • The parasite Plasmodium falciparum, like neoplastic cells, develops resistance to multiple structurally unrelated drugs. (semanticscholar.org)
  • To better understand the interaction of HSV-1 with neoplastic cells, we inoculated three-dimensional (3D) cultures of human uveal melanoma cells with HSV-1. (asm.org)
  • The role of mouse mammary tumor virus (MMTV) as a causative agent in human breast carcinogenesis has recently been the subject of renewed interest. (biomedcentral.com)
  • Here, we show for the first time the rapid spread of mouse mammary tumor virus, MMTV(GR), in cultured human mammary cells (Hs578T), ultimately leading to the infection of every cell in culture. (biomedcentral.com)
  • Taken together, our results show that human cells can support replication of mouse mammary tumor virus. (biomedcentral.com)
  • Moreover, the infectious titer obtained on human cells was similar to the titer obtained on cultured mouse mammary tumor cells (NMuMG). (biomedcentral.com)
  • N-Nitrosomethylurea-induced mammary tumors were grown in a bilayer soft-agar culture system. (elsevier.com)
  • To this end, microarray analysis was performed on CCD-1068SK human fibroblast cells after direct co-culture with MDA-MB-231 human breast tumour cells. (uct.ac.za)
  • For development and evaluation of systems through which tumor immunity might be evaluated in man, allogeneic tumor cell-lymphocyte interactions were studied with the one-way mixed culture technique. (elsevier.com)
  • Perhaps the strongest implication for a role for NKT cells in Th1 immunity is in tumor rejection responses after exogenous IL-12 ( 9 )( 28 )( 29 )( 30 ) or α-GalCer ( 11 )( 31 ) administration. (rupress.org)
  • This study shows that induction of tumor-specific CD4 + T cells by vaccination with a specific viral T helper epitope, contained within a synthetic peptide, results in protective immunity against major histocompatibility complex (MHC) class II negative, virus-induced tumor cells. (rupress.org)
  • These results indicate the crucial role of T helper cells for optimal induction of protective immunity against MHC class II negative tumor cells. (rupress.org)
  • The inability to control tumor outgrowth can be due to inadequate T helper responses underlying poor tumor-specific immunity. (rupress.org)
  • Evidence for a role of T helper cell-mediated immunity comes from studies with genetically modified tumor cells. (rupress.org)
  • Vascular mimicry in cultured head and neck tumour cell lines. (biomedsearch.com)
  • We suggest this is common phenomenon in head and neck squamous cell carcinoma (HNSCC) cell lines and other aggressive tumour cell lines. (biomedsearch.com)
  • We present experimental evidence of vasculogenic mimicry in HNSCC cell lines and compare them with other tumours and a positive control vascular cell line. (biomedsearch.com)
  • Other tumour cell lines also displayed this property. (biomedsearch.com)
  • Characterization of several clonal lines of cultured Leydig tumor cells: gonadotropin receptors and steroidogenic responses. (nih.gov)
  • Several clonal lines of cultured Leydig tumor cells have been established and characterized in terms of gonadotropin receptors and steroid production. (nih.gov)
  • Although freshly isolated cells derived from the M5480P tumor have functional hCG receptors, only two of the five clonal lines established were shown to bind significant quantities of hCG. (nih.gov)
  • In future research, Hughes-Fulford will be looking at the overall effect of different types of fatty acids on different tumor types in cell lines as well as human biopsies. (ucsf.edu)
  • Protein extracts were fractionated on Sephadex G-50 columns, and prostate tumor cell lines DU-145, LNCaP, PC-3, and HPC8L (established in our laboratory from a tumor resected from an African American patient) were treated with MBP extracts from the pooled third peaks. (scirp.org)
  • The focus of this study was to examine the CTC-iChip and determine if the device could properly capture CTCs to aid the formation of cell lines to represent the genetic status of all existing tumor sites. (oncologynurseadvisor.com)
  • Cell lines with longevity were successfully established from 6 patients. (oncologynurseadvisor.com)
  • Additional samples from 3 of these patients were used to set up new cell lines to track the effectiveness of therapy via tumor change. (oncologynurseadvisor.com)
  • Our experimental research benefits from the unrivalled collection of leukemia and lymphoma cell lines stocked at the DSMZ. (dsmz.de)
  • Genomic Landscape of Primary Mediastinal B-Cell Lymphoma Cell Lines. (dsmz.de)
  • The efficacies of two of the identified compounds, QNZ (EVP4593) and papaverine, were validated in four different tumor cell lines. (mdpi.com)
  • whereas, SF50 values were 1,000 nM or more in four (25%: resistant) cell lines. (biomedcentral.com)
  • In addition, knocking down PTEN by siRNA did not alter the sensitivity to olaparib in 2 cell lines with wild-type PTEN. (biomedcentral.com)
  • Therefore, JA induced ER stress in both cell lines, but the consequences of ER stress were different for the epidermal immortalized non-tumorigenic and tumor cells. (springer.com)
  • I: Three HER2 overexpressing cell lines were exposed to α-particles from the HER2 binding affibody molecule 211At- (ZHER2:4)2. (eurekaselect.com)
  • The division of Cell Factory, where Associate Professor Hoshi works, uses patient-derived tumor organoids (PDOs) original to Fukushima Medical University (F-PDOs), cells derived from human iPS cells, and cell lines obtained from such sources as cell banks. (essenbioscience.com)
  • Fifty cell sources, including both immortalized cell lines and primary carcinoma cells, were incubated under normoxic conditions for 48 hours. (biomedcentral.com)
  • The cell lines express either wild-type p53 (A172, CCF-STTG1, and U87MG) or mutant p53 (T98G, U118MG, and U251MG), whereas one of six cell lines overexpresses MDM2 protein (CCF-STTG1), which acts to disrupt the p53 cell cycle pathway ( Fig. 1 Refs. (aacrjournals.org)
  • The cellular uptake and metabolism of trans -AVTG in two human renal carcinoma cell lines that were used as models were rapid and associated with depletion of intracellular GSH. (aspetjournals.org)
  • Coexpression of TGFβ-1 and its receptors TGFβR I and TGFβR II was detected in 67% of human NETs and in all three NET cell lines examined. (bmj.com)
  • NET cell lines expressed the TGFβ signal transducers Smad 2, 3, and 4. (bmj.com)
  • Here, we show that pretreatment of two cervical carcinoma cell lines, HeLa and SiHa, with curcumin before ionizing radiation (IR) resulted in significant dose-dependent radiosensitization of these cells. (aspetjournals.org)
  • Peroxidation of n-3 and n-6 polyunsaturated fatty acids in the acidic tumor environment leads to ferroptosis-mediated anticancer effects. (technologynetworks.com)
  • Verjans ET, Doijen J, Luyten W, Landuyt B, Schoofs L. Three-dimensional cell culture models for anticancer drug screening: worth the effort? (eurekaselect.com)
  • No matter the tumor entity, gentleMACS Technology provides excellent cell viability and functionality for further downstream cell separation, analysis, and culture. (miltenyibiotec.com)
  • This prevents overestimation of cell viability and absolute cell count due to RBCs that falsely appear in the PI - fraction. (miltenyibiotec.com)
  • The viability of tumour spheroids was confirmed after five and ten days by using Annexin V/Propidium Iodide staining for flow-cytometry. (nih.gov)
  • Lower viability was observed in A549 monocultures compared to co-cultures, whereas Colo699 monocultures showed better viability compared to co-cultures. (nih.gov)
  • This study demonstrates that the Corning 384-well spheroid microplate can be used to generate and perform viability assays with co-culture spheroids in an easy-to-use, high throughput format. (selectscience.net)
  • Mesenchymal stem cells (MSCs) are multipotent and self-renewing progenitor cells that can differentiate into multiple mesoderm lineages. (medsci.org)
  • Rapid-onset PRDM14-induced T-ALL requires factors that are present in stem and progenitor cells: R26PR;dLck-cre animals, which express Prdm14 starting at the double-positive stage of thymocyte development, do not develop disease. (biologists.org)
  • As such, stem and progenitor cells are the likely targets for oncogenic insults that lead to transformation and subsequent conversion to CICs. (biologists.org)
  • We demonstrate an efficient approach to evaluate drug response using patient-derived circulating tumor cell (CTC) cultures obtained from liquid biopsy. (sciencemag.org)
  • To establish a sensitive and specific isolation and enumeration system for circulating tumor cells (CTC) in patients with hepatocellular carcinoma (HCC). (aacrjournals.org)
  • Detection of circulating tumor cells (CTC) has considerable clinical significance in predicting recurrence and monitoring treatment response in patients with hepatocellular carcinoma (HCC). (aacrjournals.org)
  • In collaboration with UCLouvain's Cyril Corbet, Prof. Feron demonstrated in 2020 that these same cells are the most aggressive and acquire the ability to leave the original tumour to generate metastases. (technologynetworks.com)
  • A comparison of the steroids produced by freshly isolated cells and two of the clones revealed some changes in the steroidogenic pathway. (nih.gov)
  • We found that these agents inhibited the mTOR(Mechamistic\Mammilian Target of Rapamycin) pathway in tumor cells growing under glucose starvation, but not under normal conditions. (mdpi.com)
  • Disruption of the Rb cell cycle control pathway occurs in 80% of adult GBM through loss of p16 INK4a function via gene deletion, amplification of CDK4, or mutation in Rb ( 2 , 3 ). (aacrjournals.org)
  • Natural killer (NK) cells are tightly regulated by the JAK-STAT signaling pathway and cannot survive in the absence of STAT5. (aacrjournals.org)
  • This raises the possibility that tumors might compensate for therapy directed against one pathway by upregulating a different one. (aacrjournals.org)
  • GBM cells are at least partially resistant to long-term MRK-003 treatment, despite ongoing Notch pathway suppression, and show concomitant upregulation of Wnt and Hedgehog activity. (aacrjournals.org)
  • HGF-induced anterograde lysosome trafficking depended upon the PI3K pathway, microtubules and RhoA, resulting in increased cathepsin B secretion and invasion by the cells. (biologists.org)
  • The isolated cells are then cultured on soft elastic substrates (polyacrylamide hydrogels) functionalized by an extracellular matrix protein, and embedded with fluorescent microbeads. (jove.com)
  • Isolated colon cells are then successfully cultured on soft (2 kPa stiffness) and stiff (10 kPa stiffness) polyacrylamide hydrogels and rigid polystyrene (~3.6 GPa stiffness) substrates functionalized by an extracellular matrix (fibronectin in this case). (jove.com)
  • The pseudocysts are surrounded by tumor cells and are filled with extracellular matrix components such as basement membrane molecules. (nii.ac.jp)
  • Collagen synthesis in vascular smooth muscle cells (SMCs) after exposure to tumor necrosis factor alpha (TNF-α) was investigated using a culture system. (elsevier.com)
  • However, due to defective vasculature and increased glucose uptake, tumor cells must overcome glucose deprivation. (mdpi.com)
  • LDH) release in cell supernatant and the measurement of Neutral Red (NR) uptake are used for cytotoxicity as- says. (scribd.com)