Cells or feeding stage in the life cycle of sporozoan protozoa. In the malarial parasite, the trophozoite develops from the MEROZOITE and then splits into the SCHIZONT. Trophozoites that are left over from cell division can go on to form gametocytes.
A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.
A genus of flagellate intestinal EUKARYOTES parasitic in various vertebrates, including humans. Characteristics include the presence of four pairs of flagella arising from a complicated system of axonemes and cysts that are ellipsoidal to ovoidal in shape.
A species of parasitic EUKARYOTES that attaches itself to the intestinal mucosa and feeds on mucous secretions. The organism is roughly pear-shaped and motility is somewhat erratic, with a slow oscillation about the long axis.
An infection of the SMALL INTESTINE caused by the flagellated protozoan GIARDIA LAMBLIA. It is spread via contaminated food and water and by direct person-to-person contact.
A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract.
Agents which are destructive to amebae, especially the parasitic species causing AMEBIASIS in man and animal.
Infection with any of various amebae. It is an asymptomatic carrier state in most individuals, but diseases ranging from chronic, mild diarrhea to fulminant dysentery may occur.
DYSENTERY caused by intestinal amebic infection, chiefly with ENTAMOEBA HISTOLYTICA. This condition may be associated with amebic infection of the LIVER and other distant sites.
A species of parasitic protozoa having both an ameboid and flagellate stage in its life cycle. Infection with this pathogen produces PRIMARY AMEBIC MENINGOENCEPHALITIS.
A genus of ameboid protozoa characterized by the presence of beaded chromatin on the inner surface of the nuclear membrane. Its organisms are parasitic in invertebrates and vertebrates, including humans.
Infection with amoebae of the genus ENTAMOEBA. Infection with E. histolytica causes DYSENTERY, AMEBIC and LIVER ABSCESS, AMEBIC.
Infection of the cornea by an ameboid protozoan which may cause corneal ulceration leading to blindness.
Single or multiple areas of PUS due to infection by any ameboid protozoa (AMEBIASIS). A common form is caused by the ingestion of ENTAMOEBA HISTOLYTICA.
A species of free-living soil amoebae in the family Acanthamoebidae. It can cause ENCEPHALITIS and KERATITIS in humans.
Proteins found in any species of protozoan.
A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics.
A vegetative stage in the life cycle of sporozoan protozoa. It is characteristic of members of the phyla APICOMPLEXA and MICROSPORIDIA.
A genus of ascomycetous FUNGI, family Pneumocystidaceae, order Pneumocystidales. It includes various host-specific species causing PNEUMOCYSTIS PNEUMONIA in humans and other MAMMALS.
Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.
Multinucleate cells or a stage in the development of sporozoan protozoa. It is exemplified by the life cycle of PLASMODIUM FALCIPARUM in the MALARIA infection cycle.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Infections of the brain, spinal cord, or meninges by single celled organisms of the former subkingdom known as protozoa. The central nervous system may be the primary or secondary site of protozoal infection. These diseases may occur as OPPORTUNISTIC INFECTIONS or arise in immunocompetent hosts.
The relationship between an invertebrate and another organism (the host), one of which lives at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically.
Tests that demonstrate the relative effectiveness of chemotherapeutic agents against specific parasites.
Deoxyribonucleic acid that makes up the genetic material of protozoa.
The functional hereditary units of protozoa.
Sterile solutions used to clean and disinfect contact lenses.
A genus of minute EUKARYOTES that are characterized by the preponderance of binucleate over uninucleate forms, the presence of several distinct granules in the karyosome, and the lack of a cystic stage. It is parasitic in the large intestine of humans and certain monkeys.
A free-living soil amoeba pathogenic to humans and animals. It occurs also in water and sewage. The most commonly found species in man is NAEGLERIA FOWLERI which is the pathogen for primary amebic meningoencephalitis in primates.
The continuous sequence of changes undergone by living organisms during the post-embryonic developmental process, such as metamorphosis in insects and amphibians. This includes the developmental stages of apicomplexans such as the malarial parasite, PLASMODIUM FALCIPARUM.
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Gastrointestinal infection with organisms of the genus DIENTAMOEBA.
The study of parasites and PARASITIC DISEASES.
A genus of ameboid protozoa. Characteristics include a vesicular nucleus and the formation of several lodopodia, one of which is dominant at a given time. Reproduction occurs asexually by binary fission.
A subclass of peptide hydrolases that depend on a CYSTEINE residue for their activity.
A pulmonary disease in humans occurring in immunodeficient or malnourished patients or infants, characterized by DYSPNEA, tachypnea, and HYPOXEMIA. Pneumocystis pneumonia is a frequently seen opportunistic infection in AIDS. It is caused by the fungus PNEUMOCYSTIS JIROVECII. The disease is also found in other MAMMALS where it is caused by related species of Pneumocystis.
A subfamily of the Muridae consisting of several genera including Gerbillus, Rhombomys, Tatera, Meriones, and Psammomys.
Agents used to treat trichomonas infections.
A class of amoeboid EUKARYOTES that forms either filiform subpseudopodia or lobopodia. Characteristics include the absence of sorocarps, sporangia, or similar fruiting bodies. Lobosea were formerly members of the phylum Sarcomastigophora, subphylum Sarcodina, under the old five kingdom paradigm.
Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.
Infections with unicellular organisms formerly members of the subkingdom Protozoa.
Substances that are destructive to protozoans.
A property of the surface of an object that makes it stick to another surface.
A nitroimidazole used to treat AMEBIASIS; VAGINITIS; TRICHOMONAS INFECTIONS; GIARDIASIS; ANAEROBIC BACTERIA; and TREPONEMAL INFECTIONS. It has also been proposed as a radiation sensitizer for hypoxic cells. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985, p133), this substance may reasonably be anticipated to be a carcinogen (Merck, 11th ed).
Agents used in the treatment of malaria. They are usually classified on the basis of their action against plasmodia at different stages in their life cycle in the human. (From AMA, Drug Evaluations Annual, 1992, p1585)
Ribonucleic acid in protozoa having regulatory and catalytic roles as well as involvement in protein synthesis.
One of the protein CROSS-LINKING REAGENTS that is used as a disinfectant for sterilization of heat-sensitive equipment and as a laboratory reagent, especially as a fixative.
Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
The prototypical antimalarial agent with a mechanism that is not well understood. It has also been used to treat rheumatoid arthritis, systemic lupus erythematosus, and in the systemic therapy of amebic liver abscesses.
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
A protozoan disease caused in humans by four species of the PLASMODIUM genus: PLASMODIUM FALCIPARUM; PLASMODIUM VIVAX; PLASMODIUM OVALE; and PLASMODIUM MALARIAE; and transmitted by the bite of an infected female mosquito of the genus ANOPHELES. Malaria is endemic in parts of Asia, Africa, Central and South America, Oceania, and certain Caribbean islands. It is characterized by extreme exhaustion associated with paroxysms of high FEVER; SWEATING; shaking CHILLS; and ANEMIA. Malaria in ANIMALS is caused by other species of plasmodia.
One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A tri-benzene-ammonium usually compounded with zinc chloride. It is used as a biological stain and for the dyeing and printing of textiles.
A phylum of unicellular parasitic EUKARYOTES characterized by the presence of complex apical organelles generally consisting of a conoid that aids in penetrating host cells, rhoptries that possibly secrete a proteolytic enzyme, and subpellicular microtubules that may be related to motility.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Substances used on inanimate objects that destroy harmful microorganisms or inhibit their activity. Disinfectants are classed as complete, destroying SPORES as well as vegetative forms of microorganisms, or incomplete, destroying only vegetative forms of the organisms. They are distinguished from ANTISEPTICS, which are local anti-infective agents used on humans and other animals. (From Hawley's Condensed Chemical Dictionary, 11th ed)
The N-acetyl derivative of galactosamine.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
Malaria caused by PLASMODIUM FALCIPARUM. This is the severest form of malaria and is associated with the highest levels of parasites in the blood. This disease is characterized by irregularly recurring febrile paroxysms that in extreme cases occur with acute cerebral, renal, or gastrointestinal manifestations.
11- to 14-membered macrocyclic lactones with a fused isoindolone. Members with INDOLES attached at the C10 position are called chaetoglobosins. They are produced by various fungi. Some members interact with ACTIN and inhibit CYTOKINESIS.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Proteins that contain an iron-porphyrin, or heme, prosthetic group resembling that of hemoglobin. (From Lehninger, Principles of Biochemistry, 1982, p480)
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
A benzimidazole that acts by interfering with CARBOHYDRATE METABOLISM and inhibiting polymerization of MICROTUBULES.
Change in the surface ANTIGEN of a microorganism. There are two different types. One is a phenomenon, especially associated with INFLUENZA VIRUSES, where they undergo spontaneous variation both as slow antigenic drift and sudden emergence of new strains (antigenic shift). The second type is when certain PARASITES, especially trypanosomes, PLASMODIUM, and BORRELIA, survive the immune response of the host by changing the surface coat (antigen switching). (From Herbert et al., The Dictionary of Immunology, 4th ed)
Rendering pathogens harmless through the use of heat, antiseptics, antibacterial agents, etc.
The heath plant family of the order Ericales, subclass Dilleniidae, class Magnoliopsida that are generally shrubs or small trees. Leaves are alternate, simple, and leathery; flowers are symmetrical with a 4- or 5-parted corolla of partly fused petals.
Invertebrate organisms that live on or in another organism (the host), and benefit at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically.
A protozoan parasite that occurs primarily in subtropical and temperate areas. It is the causal agent of quartan malaria. As the parasite grows it exhibits little ameboid activity.
A genus of protozoa that comprise the malaria parasites of mammals. Four species infect humans (although occasional infections with primate malarias may occur). These are PLASMODIUM FALCIPARUM; PLASMODIUM MALARIAE; PLASMODIUM OVALE, and PLASMODIUM VIVAX. Species causing infection in vertebrates other than man include: PLASMODIUM BERGHEI; PLASMODIUM CHABAUDI; P. vinckei, and PLASMODIUM YOELII in rodents; P. brasilianum, PLASMODIUM CYNOMOLGI; and PLASMODIUM KNOWLESI in monkeys; and PLASMODIUM GALLINACEUM in chickens.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
A species of TRICHOMONAS that produces a refractory vaginal discharge in females, as well as bladder and urethral infections in males.
PHENOTHIAZINES with an amino group at the 3-position that are green crystals or powder. They are used as biological stains.
ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
Adherence of cells to surfaces or to other cells.
The complete genetic complement contained in a set of CHROMOSOMES in a protozoan.
An inflammatory process involving the brain (ENCEPHALITIS) and meninges (MENINGITIS), most often produced by pathogenic organisms which invade the central nervous system, and occasionally by toxins, autoimmune disorders, and other conditions.
Zygote-containing cysts of sporozoan protozoa. Further development in an oocyst produces small individual infective organisms called SPOROZOITES. Then, depending on the genus, the entire oocyst is called a sporocyst or the oocyst contains multiple sporocysts encapsulating the sporozoites.
Infections of the INTESTINES with PARASITES, commonly involving PARASITIC WORMS. Infections with roundworms (NEMATODE INFECTIONS) and tapeworms (CESTODE INFECTIONS) are also known as HELMINTHIASIS.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
A species of PLASMODIUM causing malaria in rodents.
The concentration of a compound needed to reduce population growth of organisms, including eukaryotic cells, by 50% in vitro. Though often expressed to denote in vitro antibacterial activity, it is also used as a benchmark for cytotoxicity to eukaryotic cells in culture.

Transport of the essential nutrient isoleucine in human erythrocytes infected with the malaria parasite Plasmodium falciparum. (1/135)

The intraerythrocytic malaria parasite derives much of its requirement for amino acids from the digestion of the hemoglobin of its host cell. However, one amino acid, isoleucine, is absent from adult human hemoglobin and must therefore be obtained from the extracellular medium. In this study we have characterized the mechanisms involved in the uptake of isoleucine by the intraerythrocytic parasite. Under physiologic conditions the rate of transport of isoleucine into human erythrocytes infected with mature trophozoite-stage Plasmodium falciparum parasites is increased to approximately 5-fold that in uninfected cells, with the increased flux being via the new permeability pathways (NPPs) induced by the parasite in the host cell membrane. Transport via the NPPs ensures that protein synthesis is not rate limited by the flux of isoleucine across the erythrocyte membrane. On entering the infected erythrocyte, isoleucine is taken up into the parasite via a saturable, ATP-, Na+-, and H+-independent system which has the capacity to mediate the influx of isoleucine in exchange for leucine (liberated from hemoglobin). The accumulation of radiolabeled isoleucine within the parasite is mediated by a second (high-affinity, ATP-dependent) mechanism, perhaps involving metabolism and/or the concentration of isoleucine within an intracellular organelle.  (+info)

Effect of caspofungin on trophozoites and cysts of three species of Acanthamoeba. (2/135)

OBJECTIVES: Amoebic keratitis is difficult to treat, without total efficacy in some patients because of cysts that are less susceptible than trophozoites to the usual treatments. We investigated here the in vitro effectiveness of caspofungin, a new antifungal, against three species of Acanthamoeba. METHODS: Trophozoites and cysts of Acanthamoeba castellanii, Acanthamoeba culbertsoni and Acanthamoeba polyphaga were incubated with caspofungin at concentrations varying from 16 to 500 mg/L. RESULTS: The trophozoites of the three tested species were susceptible in vitro to caspofungin at a concentration of 250 mg/L (206 microM). Furthermore, this drug was cysticidal at a concentration of 500 mg/L (412 microM) against A. castellanii and A. culbertsoni. CONCLUSIONS: Caspofungin could represent, if in vivo studies confirm its efficacy, a new anti-Acanthamoeba compound.  (+info)

Characterization of the Plasmodium falciparum M17 leucyl aminopeptidase. A protease involved in amino acid regulation with potential for antimalarial drug development. (3/135)

Amino acids generated from the catabolism of hemoglobin by intra-erythrocytic malaria parasites are not only essential for protein synthesis but also function in maintaining an osmotically stable environment, and creating a gradient by which amino acids that are rare or not present in hemoglobin are drawn into the parasite from host serum. We have proposed that a Plasmodium falciparum M17 leucyl aminopeptidase (PfLAP) generates and regulates the internal pool of free amino acids and therefore represents a target for novel antimalarial drugs. This enzyme has been expressed in insect cells as a functional 320-kDa homo-hexamer that is optimally active at neutral or alkaline pH, is dependent on metal ions for activity, and exhibits a substrate preference for N-terminally exposed hydrophobic amino acids, particularly leucine. PfLAP is produced by all stages in the intra-erythrocytic developmental cycle of malaria but was most highly expressed by trophozoites, a stage at which hemoglobin degradation and parasite protein synthesis are elevated. The enzyme was located by immunohistochemical methods and by transfecting malaria cells with a PfLAP-green fluorescent protein construct, to the cytosolic compartment of the cell at all developmental stages, including segregated merozoites. Amino acid dipeptide analogs, such as bestatin and its derivatives, are potent inhibitors of the protease and also block the growth of P. falciparum malaria parasites in culture. This study provides a biochemical basis for the antimalarial activity of aminopeptidase inhibitors. Availability of functionally active recombinant PfLAP, coupled with a simple enzymatic readout, will aid medicinal chemistry and/or high throughput approaches for the future design/discovery of new antimalarial drugs.  (+info)

Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence. (4/135)

The pathogenic mechanism of granulomatous amebic encephalitis (GAE) and amebic keratitis (AK) by Acanthamoeba has yet to be clarified. Protease has been recognized to play an important role in the pathogenesis of GAE and AK. In the present study, we have compared specific activity and cytopathic effects (CPE) of purified 33 kDa serine proteinases from Acanthamoeba strains with different degree of virulence (A. healyi OC-3A, A. lugdunensis KA/E2, and A. castellanii Neff). Trophozoites of the 3 strains revealed different degrees of CPE on human corneal epithelial (HCE) cells. The effect was remarkably reduced by adding phenylmethylsulfonylfluoride (PMSF), a serine proteinase inhibitor. This result indicated that PMSF-susceptible proteinase is the main component causing cytopathy to HCE cells by Acanthamoeba. The purified 33 kDa serine proteinase showed strong activity toward HCE cells and extracellular matrix proteins. The purified proteinase from OC-3A, the most virulent strain, demonstrated the highest enzyme activity compared to KA/E2, an ocular isolate, and Neff, a soil isolate. Polyclonal antibodies against the purified 33 kDa serine proteinase inhibit almost completely the proteolytic activity of culture supernatant of Acanthamoeba. In line with these results, the 33 kDa serine proteinase is suggested to play an important role in pathogenesis and to be the main component of virulence factor of Acanthamoeba.  (+info)

Effects of human serum on Balamuthia mandrillaris interactions with human brain microvascular endothelial cells. (5/135)

Balamuthia mandrillaris is a free-living amoeba and a causative agent of fatal granulomatous encephalitis. In the transmission of B. mandrillaris into the central nervous system (CNS), haematogenous spread is thought to be the primary step, followed by blood-brain barrier penetration. The objectives of the present study were (i) to determine the effects of serum from healthy individuals on the viability of B. mandrillaris, and (ii) to determine the effects of serum on B. mandrillaris-mediated blood-brain barrier perturbations. It was determined that normal human serum exhibited limited amoebicidal effects, i.e. approximately 40 % of trophozoites were killed. The residual subpopulation, although viable, remained static over longer incubations. Using human brain microvascular endothelial cells (HBMEC), which form the blood-brain barrier, it was observed that B. mandrillaris exhibited binding (>80 %) and cytotoxicity (>70 %) to HBMEC. However, normal human serum exhibited more than 60 % inhibition of B. mandrillaris binding and cytotoxicity to HBMEC. ELISAs showed that both serum and saliva samples exhibit the presence of anti-B. mandrillaris antibodies. Western blots revealed that normal human serum reacted with several B. mandrillaris antigens with approximate molecular masses of 148, 115, 82, 67, 60, 56, 44, 42, 40 and 37 kDa. Overall, the results demonstrated that normal human serum has inhibitory effects on B. mandrillaris growth and viability, as well as on their binding and subsequent cytotoxicity to HBMEC. A complete understanding of B. mandrillaris pathogenesis is crucial to develop therapeutic interventions and/or to design preventative measures.  (+info)

Erythrocyte G protein as a novel target for malarial chemotherapy. (6/135)

BACKGROUND: Malaria remains a serious health problem because resistance develops to all currently used drugs when their parasite targets mutate. Novel antimalarial drug targets are urgently needed to reduce global morbidity and mortality. Our prior results suggested that inhibiting erythrocyte Gs signaling blocked invasion by the human malaria parasite Plasmodium falciparum. METHODS AND FINDINGS: We investigated the erythrocyte guanine nucleotide regulatory protein Gs as a novel antimalarial target. Erythrocyte "ghosts" loaded with a Gs peptide designed to block Gs interaction with its receptors, were blocked in beta-adrenergic agonist-induced signaling. This finding directly demonstrates that erythrocyte Gs is functional and that propranolol, an antagonist of G protein-coupled beta-adrenergic receptors, dampens Gs activity in erythrocytes. We subsequently used the ghost system to directly link inhibition of host Gs to parasite entry. In addition, we discovered that ghosts loaded with the peptide were inhibited in intracellular parasite maturation. Propranolol also inhibited blood-stage parasite growth, as did other beta2-antagonists. beta-blocker growth inhibition appeared to be due to delay in the terminal schizont stage. When used in combination with existing antimalarials in cell culture, propranolol reduced the 50% and 90% inhibitory concentrations for existing drugs against P. falciparum by 5- to 10-fold and was also effective in reducing drug dose in animal models of infection. CONCLUSIONS: Together these data establish that, in addition to invasion, erythrocyte G protein signaling is needed for intracellular parasite proliferation and thus may present a novel antimalarial target. The results provide proof of the concept that erythrocyte Gs antagonism offers a novel strategy to fight infection and that it has potential to be used to develop combination therapies with existing antimalarials.  (+info)

Transcriptional analysis of three major putative phosphatidylinositol kinase genes in a parasitic protozoan, Giardia lamblia. (7/135)

The current investigation evaluates the expression of phosphatidylinositol kinase (PIK) genes in the parasitic protozoan, Giardia lamblia. The G. lamblia Genome Database revealed the presence of two putative phosphatidylinositol-3-kinase (gPI3K) and one phosphatidylinositol-4-kinase (gPI4K) genes resembling the catalytic subunit of eukaryotic PIKs. Primers, designed to amplify mRNA of these three genes, were used to measure transcription by quantitative reverse-transcriptase polymerase chain reactions. Results suggest that all three PIK genes are expressed in non-encysting and encysting trophozoites. The relative levels of the mRNA were highest in parasites cultured in pre-encysting medium that contained no bile. Two inhibitors of PI3K, LY 294002 and wortmannin were found to inhibit the growth of the trophozoite in culture. However, wortmannin was more effective than LY294002. Altogether, the present study indicates that Giardia is capable of expressing PIKs that are necessary for the growth and differentiation of this pathogen.  (+info)

The 29-kilodalton thiol-dependent peroxidase of Entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress. (8/135)

The 29-kDa surface antigen (thiol-dependent peroxidase; Eh29) of Entamoeba histolytica exhibits peroxidative and protective antioxidant activities. During tissue invasion, the trophozoites are exposed to oxidative stress and need to deal with highly toxic reactive oxygen species (ROS). In this investigation, attempts have been made to understand the role of the 29-kDa peroxidase gene in parasite survival and pathogenesis. Inhibition of eh29 gene expression by antisense RNA technology has shown approximately 55% inhibition in eh29 expression, maximum ROS accumulation, and significantly lower viability in 29-kDa downregulated trophozoites during oxidative stress. The cytopathic and cytotoxic activities were also found to decrease effectively in the 29-kDa downregulated trophozoites. Size of liver abscesses was substantially lower in hamsters inoculated with 29-kDa downregulated trophozoites compared to the normal HM1:IMSS. These findings clearly suggest that the 29-kDa protein of E. histolytica has a role in both survival of trophozoites in the presence of ROS and pathogenesis of amoebiasis.  (+info)

In this study we performed light, immunofluorescent and transmission electron microscopy of Colpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguish Colpodella life cycle stages has not been performed previously. Rhoptry and β-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage of Colpodella (ATCC 50594). We report novel data identifying doughnut-shaped vesicles in the cytoplasm and apical end of Colpodella trophozoites reactive with antibodies specific to Plasmodium merozoite rhoptry proteins. Giemsa staining and immunofluorescent microscopy identified different developmental stages of Colpodella trophozoites, with the presence or absence of vesicles corresponding to maturity of the trophozoite. These data demonstrate for the first time evidence of rhoptry protein conservation between Plasmodium and Colpodella and ...
In this study, some differences between heme polymerase activity in rings and trophozoites of P. falciparum have been detected. Although equal numbers (2.7 × 107) of infected erythrocytes were used, mean hemozoin synthesis in ring hemolysate was only 50% of that in trophozoites. The initial quantity of hemozoin in the lysates was also lower in the rings than in the trophozoites, but Slater and Cerami (1992) have shown that hemozoin by itself does not have heme polymerase activity. They showed that heme polymerase activity increased linearly with protein concentration in parasite lysate. Obviously, hemoglobin-free P. falciparumparasites are expected to contain less protein at the ring than at the trophozoite stage, and this may account for the differences in hemozoin synthesis observed in the present study. However, Dorn et al. (1995) have shown that protein-free hemozoin can promote formation of additional hemozoin. In the present study, there was more preexisting hemozoin in the trophozoite ...
In vitro studies have proved the presence of epitopes of CD59 in the surface of trophozoites of Entamoeba histolytica (E. histolytica). However, it has not been proved if CD59 molecules are expressed in the surface during the trophozoites tissue invasion. The aim of the present study was to determine whether the complement-regulatory protein CD59 is present on trophozoites of E. histolytica in human colon. Eleven specimens of amoebic colitis were studied by immunohistochemistry and electron microscopy techniques with a monoclonal antibody against human CD59 molecule. Our results show that a CD59-like molecule is expressed in trophozoites of E. histolytica found in colonic amebic lesions. Also, a CD59-like molecule was detected by western blot analysis in whole lysate of E. histolytica as well as on the plasma membrane by immunocytochemistry. These results suggest that E. histolytica can use CD59-like protein against the lytic action of membrane attack complex.
Background Strongyloides stercoralis is an intestinal nematode that usually causes asymptomatic infection in immunocompetent individuals and reverts to life-threatening hyperinfection in immunocompromised individuals. Objective The aim of the study was to evaluate the effect of ivermectin (IVM) treatment on S. stercoralis larvae in-vitro cultured forms. In-vivo evaluation was performed by assessment of parasitological, histopathological, and ultrastructural changes in the lungs of mice with Strongyloides hyperinfection syndrome before and after treatment with IVM. Materials and methods S. stercoralis larvae were collected from agar plates cultures of positive stool samples from different areas in Menuofyia Governorate. The in-vitro study involved the examination of S. stercoralis larvae grown in agar plates (APC) after exposure for 2 h to IVM (15 μl/ml) by scanning electron microscope (SEM) and after 24 h for larval motility. In-vivo study involved 96 mice that were divided into four groups (24 ...
Background Giardia lamblia trophozoites colonize in the upper small intestine resulting in diarrhea and various clinical manifestations, including abdominal pain, anorexia, and signs of malabsorption. A decrease in the level of trace elements might occur because of this absorption deficiency resulting from giardiasis. Experimentally, the excretory secretory product of G. lamblia trophozoites increased the level of reactive oxygen species in mice enterocytes. The levels of bilirubin, uric acid, and albumin are often used as major nonenzymatic oxidative biomarkers. Objective This study was designed to determine the effect of therapy by metronidazole (MTZ) and artemether (ART) on trophozoite and cyst forms in experimentally Giardia spp.-infected hamsters and to reveal the changes in iron (Fe), manganese (Mn), copper (Cu), and chromium (Cr) serum levels pretreatment and post-treatment. Another objective was to evaluate the impact of this therapy on serum levels of bilirubin, uric acid, and albumin ...
G. Penkler, F. Du Toit, W. Adams, M. Rautenbach, D. C. Palm, D. D. Van Niekerk, & J. L. Snoep. (2014). Glucose metabolism in Plasmodium falciparum trophozoites. FAIRDOMHub. http://doi.org/10.15490/seek.1.investigation.56 ...
PfAP2-I Is a Nuclear Protein that May Bind a Conserved TGCA DNA Motif Upstream of Some Invasion Genes. Live fluorescence microscopy of synchronized parasites shows that PfAP2-I-GFP (see Figure S1B) localizes to the nucleus of trophozoite and schizont stage parasites but is not detected in ring stages. Hoechst was used as a nuclear marker. Live microscopy and nuclear fractionation assays show that PfAP2-I localizes exclusively to the nucleus of trophozoite and schizont stage parasites.Santos JM, Josling G, Ross P, Joshi P, Orchard L, Campbell T, Schieler A, Cristea IM, Llinás M. Red Blood Cell Invasion by the Malaria Parasite Is Coordinated by the PfAP2-I Transcription Factor. Cell Host Microbe. 2017 21(6).. See original on MMP ...
Localization of the antigen binding to antibody EH5 in E. histolytica trophozoites by indirect immunofluorescence using confocal microscopy. (A) Amebas treate
Supplementary MaterialsFIGURE S1: The PfDis3-ADARcd reproducibly edits particular sites in through the IDC. the editing rate of recurrence in the transcripts with an increase of than one edit sites. Picture_1.JPEG (1.6M) GUID:?DA3700B1-A11E-4C9E-ACF2-4216D199F6B4 FIGURE S2: Reproducibility of PfDis3-RIP assay across developmental phases. Relationship of genic RIP indicators in feeling (s) transcripts and antisense (as) transcripts Rabbit Polyclonal to IRAK2 between natural replicates. Pearson relationship coefficients between natural replicates are shown at top remaining part. The inset Venn diagram displaying the overlap of PfDis3 focuses on determined by PfDis3-RIP between your two replicates. R, T, S shows Ring, Schizont and Trophozoite stage, respectively. Picture_2.jpg (1.3M) GUID:?3CE2FFB1-19A0-4EC3-8727-049FC475ADF8 FIGURE S3: Functions of PfDis3-TRIBE target genes through the IDC in strategies of RIP-seq, HITS-CLIP, or GoldCLIP because of the high history and complicated manipulation ...
The photograph on the left is an iron haematoxylin stained amoeba trophozoite from a fecal smear. The patient had periodic bouts of diarrhea, abdominal cramping, and some blood in the stool. The parasite measures about 25 micrometers in length. What species is represented ...
Compared with many protists, Giardia lamblia has a simple life cycle alternating between cyst and trophozoite. Most research on the molecular biology of Giardia parasites has focused on trophozoites and the processes of excystation and encystation, whereas cysts have attracted less interest. The striking morphological differences between the dormant cyst and the rapidly dividing and motile trophozoite implies profound changes in the metabolism as the parasite encysts in the hosts intestine and excysts upon ingestion by a new host. To investigate the magnitude of the transcriptional changes occurring during the G. lamblia life cycle we compared the transcriptome of G. lamblia trophozoites and cysts using single-color oligonucleotide microarrays. Cysts were found to possess a much smaller transcriptome, both in terms of mRNA diversity and abundance. Genes encoding proteins related to ribosomal functions are highly over-represented. The comparison of the transcriptome of cysts generated in culture or
Abstract The distribution of peroxidase-labeled normal or specific rabbit immunoglobulin (Ig) in Entamoeba histolytica trophozoites was studied by transmission electron microscopy. Small amounts of normal Ig became attached to the cell surface but did not redistribute. Internalized specific Ig was firmly bound to the inner membranes of phagocytosis vacuoles, while aggregates of normal Ig were dispersed over the vacuolar lumen. The distribution of fluorescein isothiocyanate-labeled Ig in living amebae was correlated with the cell motility at different times. Immobilization coincided with the cellular metabolic processing of internalized material. Remobilization occurred when the Ig was degraded and antibodies again bound to the reappearing surface antigens. E. histolytica activated complement by the classical pathway. Fresh guinea pig serum alone did not produce lysis which, however, did occur when it was added together with normal rabbit Ig. Normal rabbit Ig may constitute a complement-fixing substrate
Entamoeba is a protozoan parasite that thrives in its niche by alternating its two forms between a proliferative trophozoite and dormant cyst. Cysts are the only form able to transmit to a new host and are differentiated from trophozoites in a process termed encystation. During Entamoeba encystation, cell metabolites, components, and morphology drastically change, which occur sequentially in an orchestrated manner.... ...
The parasite has 2 stages in its life cycle: the trophozoite and the cyst. The cyst is the resting, resistant stage and is the infectious ingested form. The trophozoite form causes manifestations of infection. The cysts are spherical or ovoid with a diameter of 40 to 60 μm and remain viable at room temperature for at least 2 weeks, particularly if kept moist and away from direct sunlight. The motile trophozoite is the form for division. Its shape and size vary with the amount of ingested food, from 30 to 300 μm in length and 30 to 100 μm in width. ...
This video shows the characteristic falling leaf motility of Giardia. I really like how you can see the shape of the trophozoite in this video. Note in the still shots below that is somewhat flattened laterally and therefore looks like a leaf. It helps explain the pattern of motility that we see ...
Fresh diarrhoeic specimen: Try to find Giardia lamblia trophozoites. Generally difficult to detect as they attach themselves to the wall of the intestine. A Giemsa or Fields stained faecal smear should be examined if giardiasis is suspected but no trophozoites are detected in a wet mount of the faeces ...
Without confirmation using the specific immunoassay to detect the true pathogen, E. histolytica, the report must indicate: Entamoeba histolytica/E. dispar group (indicate cysts and/or trophozoites); make sure your clients know what this report means in terms of pathogens/nonpathogen (based on trophozoite morphology). If cysts are seen containing a single nucleus and the typical inclusion body, then the organism could be identified to the correct species: Entamoeba polecki. Report Comment: Submit a fresh stool if you want confirmation of the true pathogen (Entamoeba histolytica). The laboratory will then test the fresh stool (fresh, frozen, some acceptable in Cary-Blair) for the presence of the true pathogen, Entamoeba histolytica, antigen. If confirmation of E. histolytica is not performed, then the physician will usually treat if the patient is symptomatic.. Treatment: ...
A total of 90 stool sample was collected from patients with gastroenteritis to detect adenovirus antigen among diarrhea cases. They were tested by general stool examination (GSE), rapid immunochromatographic test and Enzyme Linked Immunosorbent Assay (ELISA). GSE showed that adenovirus gastroenteritis infection resulted in non-bloody diarrhea, the existence of RBCs in 7% and Pus in 37% of the samples, Entamoeba histolytica trophozoite and cyst were seen in 3% and 2% of the samples respectively. The rapid test showed that 21% of samples were positive for rotavirus, 8% for adenovirus and 3% for astrovirus. Meanwhile, the ELISA test showed that adenovirus was positive in 9% of the samples. These findings established the evidence that adenovirus is significantly the second viral agent that cause gastroenteritis among children after rotavirus. Furthermore, it must be stated that rapid diagnostic methods are important for detection of adenovirus infection.. ...
Giardia lamblia protozoan. Coloured transmission electron micrograph (TEM) of a sagittal section through a Giardia lamblia protozoan (green/pink) attached to the microvilli (pink) in the intestine. G. lamblia is a parasite that causes the intestinal illness giardiasis. It exists first as an infectious cyst (not seen), entering the intestine via contaminated water or food. It then develops into a trophozoite, which multiplies in the intestine and blocks nutrient absorption by covering the surface of the intestine and/or damaging the microvilli, the absorptive structures in the intestine. Symptoms of giardiasis include diarrhoea and abdominal cramps. - Stock Image Z100/0193
Most species in this family infect the Malpigian tubes of beetles. The trophozoite is conical in shape. Two types of schizogony occur in this family. In the first type the schizonts divide into merozoites with small nuclei. These are known as mycetoids merozoites. These develop into trophozoites. In the second form and more common form, the schizonts divide into merozoites with large nuclei: these are known as gregarinoid merozoites and give rise to gametocytes. The merozoites are uninucleated, pyriform cells. These are released into the lumen of the tube and from there infect other cells of the tube. The gametocytes possesses only a single nucleus and are globular in shape. When mature, these become detached from the epithelium. Within the lumen, the gametocytes associate in pairs, fuse and form a zygote. The zygote subsequently becomes a single octozoic spore. ...
Expression of arginine-metabolizing enzymes in IECs upon Giardia infection. Differentiated Caco-2 IECs were in vitro infected with Giardia trophozoites of three
Giardiasis is the most common cause of acute protozoan intestinal infection worldwide, and chronic giardiasis is a major contributor to high morbidity in develo...
Giardia lamblia, суммарные антитела -, Серологические и иммунохимические исследования - 630 руб.
Math 30 IB Standard Level Body Mass Index Portfolio Assignment Words May 4th, 11 Pages This portfolio is an investigation into how the median Body Mass Index of a girl will change as she ages.
This portfolio is an investigation into how the median Body Mass Index of a girl will change as she ages. Body Mass Index (BMI) is a comparison between a persons height (in meters) and weight (in kilograms) in order to determine whether one is overwei...
Os ywr person sydd wedii heintio mewn gr?p risg uchel e.e. yn trin bwyd, yn gweithio mewn meithrinfa, yn nyrs neun gofalu am yr henoed ac ati, ni allant ddychwelyd ir gwaith tan iddynt wellan llwyr am 48 awr. Ar adegau bydd angen sicrhau canlyniadau negyddol i samplau o garthion. Hefyd, maen bosibl y bydd angen gwahardd rhai plant heintiedig o grwpiau chwarae, meithrinfeydd, gofalwyr plant neu ysgolion. Bydd swyddog or adran yn rhoi gwybod i chi os bydd angen gwahardd a phryd y gallwch ailddechrau gweithgareddau ...
Parazitologia: coproparazitologic (ouă, larve paraziți, Giardia lamblia), anticorpi parazitari (trichineloză, toxoplasmoză, toxocaroză, echinococoză).
Accepted November 29, 2011.. Abstract. The MeOH extract of Cupania dentata bark (Sapindaceae) as well as its hexane, CH2Cl2, EtOAc, and BuOH fractions showed high activity against Giardia lamblia trophozoites (IC50 = 2.12 9.52 μg/mL). The phytochemical study of fractions resulted in the isolation of taraxerone (1), taraxerol (2), scopoletin (3), and two mixtures of steroidal compounds. Taraxerone was the metabolite with the highest giardicidal activity (IC50 = 11.33 μg/mL).. Key words: Cupania dentata, Sapindaceae, giardicidal activity, taraxerone, taraxerol, scopoletin, sterols.. Resumen. El extracto MeOH de Cupania dentata corteza (Sapindaceae) así como sus fracciones de hexano, CH2C12, AcOEt y BuOH mostraron gran actividad contra los trofozoítos de Giardia lamblia (CI50 = 2.12 9.52 μg/mL). El estudio fitoquímico de estas fracciones resultó en el aislamiento de taraxerona (1), taraxerol (2), escopoletina (3) y dos mezclas esteroidales. Taraxerona tuvo la más alta actividad giardicida ...
Entamoeba histolytica is an intestinal protozoan parasite of humans that causes amebic colitis and amebic liver abscess: diseases associated with significant levels of morbidity and mortality worldwide [1]. The organism has a simple life cycle, existing as either the motile trophozoite or the infectious, hardy cyst form. Trophozoites of E. histolytica reside within the anaerobic confines of the human colon, lack mitochondria, derive energy from fermentation and reproduce by binary fission. E. histolytica trophozoites can be potent pathogens, possessing an armamentarium that includes a galactose-N-acetyl-galactosamine-binding lectin that mediates adherence to host cells, pore-forming proteins (amebapores) that can lyse bacteria or eukaryotic cells, cysteine proteinases that can cleave extracellular-matrix proteins and facilitate invasion into the colonic mucosa, and impressive phagocytic capabilities [1]. During the past three decades, the tools of molecular biology have greatly increased the ...
Balantidium coli is a parasitic species of ciliate alveolates that causes the disease balantidiasis. It is the only member of the ciliate phylum known to be pathogenic to humans. Balantidium coli has two developmental stages, a trophozoite stage and a cyst stage. In trophozoites, the two nuclei are visible. The macronucleus is long and sausage-shaped, and the spherical micronucleus is nested next to it, often hidden by the macronucleus. The opening, known as the peristome, at the pointed anterior end leads to the cytostome, or the mouth. Cysts are smaller than trophozoites and are round and have a tough, heavy cyst wall made of one or two layers. Usually only the macronucleus and sometimes cilia and contractile vacuoles are visible in the cyst. Living trophozoites and cysts are yellowish or greenish in color. Balantidium is the only ciliated protozoan known to infect humans. Balantidiasis is a zoonotic disease and is acquired by humans via the feco-oral route from the normal host, the pig, where ...
A Detailed, Hierarchical Study of Giardia lamblias Ventral Disc Reveals Novel Microtubule-Associated Protein Complexes Journal Article ...
Giardiasis is the gastrointestinal illness caused by the flagellated protozoan Giardia intestinalis, also known as G. lamblia or G. duodenalis. Giardia is the most commonly diagnosed intestinal parasite in public health laboratories in the United States (1--4). Giardia was the most frequently identified etiologic agent of outbreaks associated with drinking water in the United States for the years 1976--1994 (5). Giardia is spread from person to person and from animals to humans through fecal-oral transmission, has an incubation period of 3--25 days (median, 7--10 days), and has a two-stage life cycle --- trophozoite and cyst. The life cycle begins with ingested cysts, which release trophozoites (10--20 µm x 5--15 µm) in the duodenum. These trophozoites attach to the surface of the intestinal epithelium by using a ventral sucking disk and then reproduce by binary fission. The trigger for encystment is unclear, but the process results in the inactive, environmentally resistant form of Giardia ...
Looking for Giardia lamblia? Find out information about Giardia lamblia. A genus of zooflagellates that inhabit the intestine of numerous vertebrates, and may cause diarrhea in humans Explanation of Giardia lamblia
It can be difficult to diagnose Giardia in dogs, because the protozoa are very, very small and they dont appear in every bowel movement. Your vet may need to perform multiple fecal examinations to diagnose Giardia. Vets use special procedures to identify Giardia. These procedures are the same as the ones used to diagnose hookworms and roundworms. These procedures kill trophozoites and can help make encysted protozoa easier to find. Your vet will mix some of your dogs stool with water and look at it under a microscope to search for trophozoites. Because of their flagella, trophozoites can be seen moving under a microscope. Cysts are often found in firm bowel movements, and can be isolated using a special solution. Your vet will then examine the solution under a microscope. Even interpreting the results of Giardia tests can be difficult, because Giardia is often present in the stools of uninfected dogs, and your dogs diarrhea symptoms may be caused by another disease entirely. Your vet will ...
Giardia lamblia protozoan, coloured scanning electron micrograph (SEM). This is a single-celled parasite of the intestinal tract. It is most common in tropical regions. This is the trophozoite (active, feeding) life stage of the protozoan. The cell has flagella (hair-like structures), which are used for locomotion. Giardia lamblia is the causative agent of giardiasis, an intestinal disease that causes abdominal pain, diarrhoea and nausea. It is spread through contaminated food and water. Treatment is with antibiotics or antiprotozoal drugs, such as metronidazole. - Stock Image Z100/0235
activity. Late trophozoite stage parasites were incubated with rabbit polyclonal anti-P12 or anti-P41 antibodies either independently or in combination. Final
Giardia atau lengkapnya Giardia lamblia, Giardia intestinalis, atau Giardia lamblia intestinalis duodenalis adalah parasit protozoa ber-flagela yang berkoloni di usus manusia dan hewan. Infeksi giardia pada manusia dapat menyebabkan kondisi yang disebut giardiasis.. ...
(2006) Davids et al. PLoS ONE. Since the Giardia lamblia cyst wall is necessary for survival in the environment and host infection, we tested the hypothesis that it contains proteins other than the three known cyst wall proteins. Serial analysis of gene expression during growth and encystation re...
GUIMAR ES, Sem ramis, SOGAYAR, Maria In s T. Leme and FRANCO, Marcello Analysis of proteins from membrane and soluble fractions of Giardia duodenalis trophozoites of two Brazilian axenic strains. Rev. Inst. Med. trop. S. Paulo, Oct 2002, vol.44, no.5, p.239-244. ISSN 0036- ...
De asemenea, chisturile paraziților pot fi depozitate pe vase și haine. Malaria nu se poate lua doar daca stam in preajma unui pacient infectat. Garlicaps usturoi eficient în eliminarea viermilor intestinali limbrici şi oxiuri.
Vitamin A, Ability, Absorption, Adaptive Immunity, Architecture, Children, Developing Countries, Diseases, Giardia, Giardia Lamblia, Giardiasis, Health, Immunity, Infectious Diseases, Lamblia, Liver, Persons, Prevalence, Retinol, School
After 35 years striving to practice the art of medicine, Ive realized that modern medical practice has devolved into a series of business transactions. As I walk down the corridor to see my next patient, I find myself wondering how I will survive.
Scaunul materiile fecale animalelor infectate sau ale oamenilor contin parazitii. Interiorul prezintă resturi de flageli şi nuclei. Care sunt simptomele infestarii cu Giardia?
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Entamoeba histolytica (es); Entamoeba histolytica (hu); Entamoeba histolytica (eu); Entamoeba histolytica (ast); Entamoeba histolytica (ca); Entamoeba histolytica (de); Entamoeba histolytica (ga); Дизентерична амеба (sr-ec); 溶组织内阿米巴 (zh); Entamoeba histolytica (da); Entamoeba histolytica (tr); 溶組織內阿米巴 (zh-hk); Entamoeba histolytica (sv); Entamoeba histolytica (uk); Entamoeba histolytica (la); 溶組織內阿米巴 (zh-hant); 溶组织内阿米巴 (zh-cn); Suoliameba (fi); Дизентерия амебасы (kk); měňavka úplavičná (cs); Entamoeba histolytica (it); Entamoeba histolytica (fr); Entamoeba histolytica (nl); Dizenteriya amyobasi (uz); Entaméba istulìtica (eml); Entamoeba histolytica (en); Դիզենթերիայի ամեոբա (hy); Entamoeba histolytica (vi); дизентерийная амёба (ru); Entamoeba histolytica (pt); انتاموبا هیستولیتیکا (fa); 溶組織內阿米巴 (zh-tw); Entamoeba histolytica (nb); ...
The influence of inflammation on the number of trophozoites and on the murine amoebic liver abscess area following infection with Entamoeba histolytica and E. dispar was evaluated. Immunohistochemistry and digital morphometry were used to identify and quantify the trophozoites, neutrophils, macrophages, and lesions. Positive correlation was observed between the number of trophozoites and inflammatory cells. A significant decrease in parasitism and inflammation in groups treated with dexamethasone was observed. The scarceness or absence of trophozoites in the treated groups suggest the importance of the inflammatory response in the production of amoebic hepatic abscesses in spite of the inherent virulence of the parasite being decisive in the establishment of the lesion.
The protozoan parasite Entamoeba histolytica causes invasive intestinal and extraintestinal infections in about 50 million people world-wide resulting in a death toll of up to 100,000 people annually. It remains a significant cause of human death in developing countries such as Bangladesh and Vietnam. However, four out of five E. histolytica infections remain asymptomatic. What determines the outcome of an E. histolytica infection is largely unknown. The DNA content of E. histolytica grown in axenic cultures (in the absence of bacteria) is at least 10 fold higher than in xenic cultures. In turn, re-growth of axenized parasites in the presence of bacteria leads to reduction of DNA content to the original xenic values. A similar reversible increase in genomic DNA content has been described during passage from cyst to trophozoite in E. invadens, a model organism for encystations. There exists evidence to suggest that variation in genome content is the result of the accumulation of multiple copies ...
Balantidiasis is an infectious disease caused by the ciliated protozoan parasite called Balantidium coli. The life cycle of the Balantidium coli begins with cysts in the host. The host consumes contaminated water or food, developing cysts which are considered as the parasite stage that transmits Balantidiasis. Excystation then takes place within the small intestine of the host. During this stage, trophozoites inhabit the large intestine. Within the lumen of the large intestine, the trophozoites replicate through binary fission and it is possible for conjugation to take place during this stage. Encystation of the trophozoites follows resulting in infectious cysts. A number of trophozoites will occupy the colon and multiply there while others go back to the lumen of the large intestine for disintegration. Finally, mature cysts are expelled from the body of the host with feces. Balantidium coli bacterium can be found all over the world. Pigs serve as its ideal animal reservoir, thus, Balantidiasis ...
Giardia lamblia, a pathogen causing diarrhoeal outbreaks, is interesting how it triggers immune response in the human epithelial cells. This study defined the crucial roles of signalling components involved in G. lamblia-induced cytokine production in human epithelial cells. Incubation of the gastrointestinal cell line HT-29 with G. lamblia GS trophozoites triggered production of interleukin (IL)-1β, IL-8 and tumour necrosis factor (TNF)-α. IL-8 production was not significantly decreased by physically separating the HT-29 cells and G. lamblia GS trophozoites. Indeed, treatment of HT-29 with G. lamblia excretory-secretory products (ESP) induced IL-8 production. Electrophoretic mobility gel shift and transfection assays using mutagenized IL-8 promoter reporter plasmids indicated that IL-8 production by G. lamblia ESP occurs through activation of two transcriptional factors, nuclear factor kappaB (NF-κB) and activator protein 1 (AP-1) in HT-29 cells. In addition, activation of two ...
Alpha-giardins constitute the annexin proteome (group E annexins) in the intestinal protozoan parasite Giardia and, as such, represent the evolutionary oldest eukaryotic annexins. The dominance of alpha-giardins in the cytoskeleton of Giardiawith its greatly reduced actin content emphasises the importance of the alpha-giardins for the structural integrity of the parasite, which is particularly critical in the transformation stage between cyst and trophozoite. In this study, we report the crystal structures of the apo- and calcium-bound forms of a1-giardin, a protein localised to the plasma membrane of Giardia trophozoites that has recently been identified as a vaccine target. The calcium-bound crystal structure of a1-giardin revealed the presence of a type III site in the first repeat as known from other annexin structures, as well as a novel calcium binding site situated between repeats I and IV. By means of comparison, the crystal structures of three different alpha-giardins known to date ...
Peripheral blood smears were available for staging from 14 of 17 cases. Ring forms comprised the majority of parasite types observed in all cases (Fig. 1), and mature-stage parasites were observed in only 7 of 14 cases examined. Overall, 96.5% ± 1.9% (range, 79.4 to 100%) of all IEs were ring forms (Fig. 1), and no schizonts were seen. The mean parasitemia of pigmented trophozoites in placental washings was over 200 times higher than that of peripheral blood (11.2% versus 0.048%).. These findings indicate that IEs selectively accumulate in the blood spaces of the placenta. This is predominantly a feature of pigmented trophozoites, which express PfEMP1 on their surface (11) and may adhere to CSA and HA or bind immunoglobulins (4, 8, 14, 18). The intraerythrocytic parasite life cycle lasts approximately 48 h, with 24 h in the ring stage, 12 h as pigmented trophozoites, and 12 h as schizonts (20). Therefore, in the absence of selective processes, random sampling of parasites should yield ...
Most helminths and protozoa exit the body in the fecal stream.The patient should be instructed to collect feces in a clean waxed or cardboard container and to record the time of collection on the container. Contamination with water (which could contain free-living protozoa) or with urine (which can damage trophozoites) should be avoided. Fecal samples should be collected before ingestion of barium or other contrast agents for radiologic procedures and before treatment with antidiarrheal agents and antacids, because these substances change the consistency of the feces and interfere with microscopic detection of parasites. Because of the cyclic shedding of most parasites in the feces, a minimum of three samples collected on alternate days should be examined. Examination of a single sample can be up to 50% less sensitive. When delays in transport to the laboratory are unavoidable, fecal samples should be kept in polyvinyl alcohol or another fixative to preserve protozoal trophozoites. New ...
Entamoeba histolytica ATCC ® 50543™ Designation: HK-9 Clone 4 Isolation: strain HK-9 (=ATCC 30015) monoxenized and cloned via microisolation, then re-axenized
Definition of giant schizonts in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is giant schizonts? Meaning of giant schizonts as a legal term. What does giant schizonts mean in law?
Summary[edit] DescriptionAPI English: ER morphology in E. histolytica treated by nitric oxide. Trophozoites expressing GFP-KDEL-FLAG were seeded on glass bottom culture dishes (MatTeck) embedded in a collagen matrix and incubated at 37°C. Immediately before imaging 1 mM SNP was added directly to the top of the matrix that then was covered by a coverslip. The amoebas were then imaged using the Perkin Elmer Spinning Disc Confocal Microscope. All images were acquired using the 40à objective and taking one stack every minute for 1 h using a 0.5 µm Z-step. Date 2012 Source Video S2 from Santi-Rocca J, Smith S, Weber C, Pineda E, Hon C, Saavedra E, Olivos-GarcÃ-a A, Rousseau S, Dillies M, Coppée J, Guillén N (2012). Endoplasmic Reticulum Stress-Sensing Mechanism Is Activated in Entamoeba histolytica upon Treatment with Nitric Oxide. PLOS ONE. DOI:10.1371/journal.pone.0031777. PMID 22384074. PMC: 3286455. Author Santi-Rocca J, Smith S, Weber C, Pineda E, Hon C, Saavedra E, ...
A dog becomes infected by eating the cyst form of the parasite. In the small intestine, the cyst opens and releases an active form called a trophozoite. These have flagella, hair-like structures that whip back and forth allowing them to move around. They attach to the intestinal wall and reproduce by dividing in two. After an unknown number of divisions, at some stage, in an unknown location, this form develops a wall around itself (encysts) and is passed in the feces. The Giardia in the feces can contaminate the environment and water and infect other animals and people ...
Proteins expressed during this time are known in Plasmodium falciparum, Plasmodium berghei, Toxoplasma gondii.. This specific developmental stage comes under the not trophozoite umbrella.. ...
Indeed, thats what many thought-and what many still teach. In an article titled, A Homage to Giardia, Kim Nasmyth observed: To learn more about eukaryotes common ancestor, we need to know about protozoan organisms that descend from the earliest offshoots of the eukaryotic lineage. A good candidate is the archaezoan Giardia lamblia,… (1996, 6:1042). Soltys described it this way: Giardia lamblia is considered one of the deepest branching or most primitive eukaryotes in existence… (n.d.).. However, a recent study has evolutionists scrambling for a new missing link. In the report documenting this latest discovery, Tovar and colleagues noted: …here we demonstrate that Giardia contain mitochondrial remnant organelles (mitosomes) bounded by double membranes that function in iron-sulphur protein maturation. Our results indicate that Giardia is not primitively amitochondrial… (2003, 426:172, parenthetical item in orig.). Henze and Martin noted: For years, a unicellular creature ...
Flagellates are unicellular microorganisms. Their locomotion is by lashing a tail-like appendage called a flagellum or flagella and reproduction is by simple binary fission.
Some examples of flagellates include trypanosomes and Giardia lamblia. Trypanosome is a parasite that causes sleeping sickness when it comes into contact with a human. Giardia lamblia is also a...
Learning and Relearning Movement Human motor learning depends on a suite of brain mechanisms that are driven by different signals and operate on timescales ranging from minutes to years. Understanding these processes requires identifying how new ...
Giardiasis is an intestinal infection by a parasite known as Giardia lamblia. Giardia is the most common parasite to infect travelers in places with unsanitary conditions, according to Drugs.com.
Giardiasis is an intestinal illness caused by infection with the parasite Giardia lamblia, which lives in contaminated water. Although the illness most…
Giardia is a devastating parasite for humans. SureAqua products are compact, easy to use and safeguard you against the risk of water borne illnesses.
The trophozoite is conical in shape. Two types of schizogony occur in this family. In the first type the schizonts divide into ... These develop into trophozoites. In the second form and more common form, the schizonts divide into merozoites with large ...
The trophozoites are solitary. Syzygy occurs late in the life cycle. They parasitise the coelom of earthworms and are spread by ...
Trophozoites: Ring forms are unusual. The trophozoite is usually irregular in outline. The nucleus is small and there may be a ...
Young trophozoites contain prominent vacuoles. Schizonts contain 40 to 74 nuclei and have pigment. This species is found in ...
The trophozoites inhabit the gut lumen. They are cylindrical in shape with folds along the surface of the cell. The nucleus is ...
The trophozoites are characterized by a nucleus and a surrounding halo. They travel by pseudopodia, which means that they ... The trophozoite attaches to olfactory epithelium, where it follows the olfactory cell axon through the cribriform plate (in the ... N. fowleri occurs in three forms - as a cyst, a trophozoite (ameboid), and a biflagellate. It does not form a cyst in human ... Trophozoites encyst due to unfavorable conditions. Factors that induce cyst formation include a lack of food, overcrowding, ...
Trophozoite[muuda , muuda lähteteksti]. Hõivates erütrotsüüdi, kaotab parasiit invasiooniorganellid ja muundub trophozoite ...
Trophozoite[muuda , muuda lähteteksti]. Hõivates erütrotsüüdi, kaotab parasiit invasiooniorganellid ja muundub trophozoite ...
The trophozoites may develop intracellularly or extracellularly. In species where the trophozoites develop extracellularly, ...
... has two developmental stages, a trophozoite stage and a cyst stage. In trophozoites, the two nuclei are ... Once the cyst reaches the small intestine, trophozoites are produced. The trophozoites then colonize the large intestine, where ... Some trophozoites invade the wall of the colon using proteolytic enzymes and multiply, and some of them return to the lumen. In ... Living trophozoites and cysts are yellowish or greenish in color. Balantidium is the only ciliated protozoan known to infect ...
The trophozoites appear opaque and occasionally granular. Schizogony occurs in the host cell. Morphological sexual ...
The trophozoites live within the gut lumen. They measure 200-300 μm × 40-70 μm. There are 30-40 grooves along the body. Four ...
Structural observations of cultured trophozoites". Experimental Parasitology. 130 (1): 86-90. doi:10.1016/j.exppara.2011.10.009 ...
The trophozoite is aseptate. The gamont is sac-like and extends at one end into two branches which divide dichotomously, ...
Trophozoites The trophozoite shape varies among Retortamonas species. Pyriform, fusiform, and rounded structures have all been ... Retortamonas trophozoites then multiply asexually through lateral binary fission. Eventually the trophozoites encyst, and the ... The trophozoites feed by endocytosis with the help of the posterior, vaned flagellum. Movement in a waving-like motion by the ... Retortamonas trophozoites live within the intestinal tract of its host while cysts are more resilient and can survive in the ...
Young trophozoites are small and occupy three-tenths to four-tenths of the erythrocytes. The nucleus stains rose-red. Larger ... trophozoites are more irregular tending toward amoeboidity. Pigment is in granules and there is no stippling of the host cell. ...
It forms cysts with four nuclei which excyst in the body and become trophozoites. Endolimax nana nuclei have a large endosome ... In stained preparations, the nucleus has a distinct karyosome that, while not as large as that seen in the trophozoite, is ... Living trophozoites are sluggish and generally non-progressive. The single nucleus sometimes is visible in unstained ...
The trophozoites are vermiform with an apical complex. They have few epicytic folds. A dense array of microtubules lies under a ...
It has a direct action on the trophozoites. Its greater concentration and duration of action in the liver as compared to that ... It is the involvement of liver tissue by trophozoites of the organism Entamoeba histolytica and of its abscess due to necrosis ... This amoebicide acts directly on the trophozoites of E. Histolytica. Studies showed that because of very high concentration in ... trophozoites and cysts) Radiography Aspiration exploratory Medical ultrasonography and CT scanning Sigmoidoscopy Liver function ...
The trophozoites are found in the intestinal lumen. They measure 23-31 microns x 11-15 microns. They are initially ovoid and ... The trophozoites develop in multinucleated schizonts which give rise to 8-20 merozoites. The merozoites are 5-6 microns x 1 ...
Species within the genus, Acanthamoeba, are generally free-living trophozoites. These trophozoites are relatively ubiquitous ... In addition to the trophozoite stage, the organism can also form a double-walled cyst which may also be present in the ... Early use of high dose dual therapy helps to eliminate both trophozoite and cyst forms of the organism, while also preventing ... However, no single therapy has been found to eliminate both trophozoite and cystic forms, and to eradicate corneal infection. ...
The trophozoites are worm like with longitudinal folds. The schizonts are globular with multiple nuclei. The gamonts are ...
Trophozoites develop into schizonts which contain multiple merozoites. After a minimum of one week, the sexual stage begins ...
The trophozoites are irregularly shaped with filiform extensions. The schizonts contain 8 to 20 nuclei arranged in a fan. ...
Microscopic diagnosis can be difficult because the early trophozoites ("ring form") of all four species look identical and it ... species identification is always based on several trophozoites. The biggest pitfall in most laboratories in developed countries ...
The trophozoites are large, band like and wide. They have longitudinal striae and are aseptate. A mucron of small pseudopods or ... Cytokinesis occurs to form uninucleate merozoites arranged in clusters in the shape of the trophozoite. The merozoites become ... free by dropping off the parent trophozoite. Gamonts, gametocysts and oocysts are of the actinocephalid type with syzygy ...
The active (trophozoite) stage exists only in the host and in fresh loose feces; cysts survive outside the host in water, in ... Once the trophozoites are excysted in the terminal ileum region, they colonize the large bowel, remaining on the surface of the ... The trophozoites will then ingest these dead cells. This damage to the epithelial cell layer attracts human immune cells and ... The trophozoites can also end up in other organs, sometimes via the bloodstream, sometimes via liver abscess rupture or ...
Diagnosis of species can be difficult because the early trophozoites ("ring form") of all four species look similar and it is ... species identification is always based on several trophozoites. As malaria becomes less prevalent due to interventions such as ...
The trophozoites are 9-14 micrometres in diameter. Trophozoites are one of the two forms of I. buetschlii. This form has a ... trophozoite in cecum & colon-->cyst in feces- ingested-->trophozoite in cecum & colon In a research study, amebas were seen in ... Trophozoites are often identified by a stool smear, found in loose stools. The cysts are 8-10 micrometres in diameter, with a ... The trophozoite has a single nucleus, prominent for nuclear endosome and many cytoplasmic vacuoles. The ectoplasm and the ...
T. tenax trophozoites multiply by longitudinal binary fission. These trophozoites are unable to survive the digestive process. ... T. tenax trophozoites survive in the body as mouth scavengers that feed primarily on local microorganisms located between the ... The specimen of choice for diagnosing Trichomonas tenax trophozoite is mouth scrapings. Microscopic examination of tonsillar ... crypts and pyorrheal pockets of patients suffering from T. tenax infections often yields the typical trophozoites. Tartar ...
The trophozoite life stage of Giardia colonizes and proliferates in the small intestine. Trophozoites develop during the course ... The complement of the trophozoite state is the thick-walled cyst form). Trophozoite and cyst stages are shown in the life cycle ... A trophozoite (G. trope, nourishment + zoon, animal) is the activated, feeding stage in the life cycle of certain protozoa such ... In the apicomplexan life cycle the trophozoite undergoes schizogony (asexual reproduction) and develops into a schizont which ...
The proteinase activity of Giardia lamblia trophozoites, Portland 1 strain, was characterized with respect to substrate ... Giardia lamblia: characterization of proteinase activity in trophozoites Exp Parasitol. 1989 Feb;68(2):168-75. doi: 10.1016/ ... The proteinase activity of Giardia lamblia trophozoites, Portland 1 strain, was characterized with respect to substrate ...
... the amoebicidal and amoebistatic efects of Artemisia argyi leaf methanolic extract by testing the effects on trophozoites and ... The growth of trophozoites stopped in A. argyi methanolic extract with 50% inhibitory concentrations (IC50)/8 h for 37.4 mg/ ml ... In vitro amoebicidal activity of Origanum syriacum and Origanumla evigatum on Acanthamoeba castellanii cysts and trophozoites. ... In vitro amoebicidal activities of Teucrium polium and T. chamaedrys on Acanthamoeba castellanii trophozoites and cysts. ...
c) and (d) Toluidine blue stained thick resin sections of trophozoites. The morphology of the trophozoites is very diverse, ... Bar = 1.0 μm. Insert = 0.2 μm. (f) A trophozoite in a lateral view is shown in a displacing position with a portion of the cell ... d) Trophozoites (T) were found in close contact with the surface of epithelial cells (Ec) and also under the cell layer. ... Trophozoites were added in a 1 : 2 target cell : amoeba ratio in a mixture of equal proportions of Bacto Casitone and ...
In in vitro assays the hemolytic activity of total homogenates of trophozoites of seven strains of amebae were tested for ... In in vitro assays the hemolytic activity of total homogenates of trophozoites of seven strains of amebae were tested for ...
Major surface trophozoite antigen 11Add BLAST. 650. Amino acid modifications. Feature key. Position(s). DescriptionActions. ... sp,Q03185,TS11_GIAIN Major surface trophozoite antigen 11 OS=Giardia intestinalis OX=5741 GN=TSP11 PE=3 SV=1 ...
ULTRASTRUCTURE OF THE GREGARINA NIPHANDRODES NUCLEUS THROUGH STAGES FROM UNASSOCIATED TROPHOZOITES TO GAMONTS IN SYZYGY AND THE ... The nucleus goes through dramatic changes from multiple spheres of condensed chromatin in unassociated trophozoites, to mostly ... we describe the ultrastructure of the syzygy junction and the nucleus during the transition from unassociated trophozoites to ... "ULTRASTRUCTURE OF THE GREGARINA NIPHANDRODES NUCLEUS THROUGH STAGES FROM UNASSOCIATED TROPHOZOITES TO GAMONTS IN SYZYGY AND THE ...
Abstract Giardia muris has been maintained for years in the mouse by administering by gavage 10,000 trophozoites taken from the ... Oral Infection of Mice with Trophozoites of Giardia muris * Ivo de Carneri*, Franca Trane ... Giardia muris has been maintained for years in the mouse by administering by gavage 10,000 trophozoites taken from the small ...
... histolytica trophozoites. We now demonstrate that gamma interferon (IFN-γ) is produced by these stimulated lymphocytes and is ... histolytica trophozoites, respectively, over 6 hr at 37°C (P , 0.001 for each compared to control). Macrophages incubated with ... The Role of Gamma Interferon in the Generation of Human Macrophages Cytotoxic for Entamoeba histolytica trophozoites * Robert A ... histolytica trophozoites, respectively, over 6 hr at 37°C (P , 0.001 for each compared to control). Macrophages incubated with ...
Lfcin-B-treated trophozoites; (vii) Lfcin 17-30-treated trophozoites; and (viii) Lfampin-treated trophozoites. (B) Percentage ... Lfcin-B-treated trophozoites; (vii) Lfcin 17-30-treated trophozoites; and (viii) Lfampin-treated trophozoites. (B) Percentage ... E. histolytica trophozoites internalize lactoferrin (Lf)-derived peptides. Trophozoites (1 × 105) were cultured in 200 μl of ... E. histolytica trophozoites internalize lactoferrin (Lf)-derived peptides. Trophozoites (1 × 105) were cultured in 200 μl of ...
Finally, we report the successful microscopy imaging of both Giardia trophozoites and cysts with an OFM that has a focal plane ... This paper reports the adaptation of the OFM for imaging Giardia lamblia trophozoites and cysts, a disease-causing parasite ... The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts ... The application of on-chip optofluidic microscopy for imaging Giardia lamblia trophozoites and cysts. Biomedical Microdevices, ...
For trophozoite isolation, parasite and host nuclei are stained using propidium iodide (PI) followed by flow cytometry and cell ... RESULTS AND CONCLUSION: The results demonstrate that single trophozoite genotyping is possible and that cell sorting can be ... METHODS: The method described allows isolation of Plasmodium falciparum trophozoites, genotyping and whole genome sequencing ... sorting to separate trophozoites from host cells. Before genotyping or sequencing, whole genome amplification is used to ...
Trophozoite Isolation and Lysate Preparation. Assay for the isolation of intact Plasmodium falciparum trophozoites from ... SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the ... SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the ... of a detailed mathematical model for glycolysis of the malaria parasite Plasmodium falciparum in the blood stage trophozoite ...
trophozoite Giardiasis in Dogs & Cats - An Under-Appreciated Cause of Diarrhea. 2018-01-01. by CriticalCareDVM ...
Wet mount trophozoite - Giardia lamblia: This organism is a pathogenic flagellate. This animated GIF shows the view one would ... The trophozoites measure 50 to 200 microns. Viable organisms in unfixed stool samples are very active moving across the field ... see as they focus up and down through the organisms trophozoite stage in a wet mount preparation. ...
Intact trophozoite of G. duodenalis (clone P1C10) also took up and metabolised L-[U14C-] alanine to 14CO2 at rates of 20.6 ± ... Intact trophozoites of Giardia duodenalis (clone P1C10) took up and metabolised L-[U14C-] aspartate to 14CO2 at rates of 10.27 ... The uptake and conversion of l-[U14C-] aspartate and l-[U14C-] alanine to 14CO2 by intact trophozoites of Giardia duodenalis ... Trophozoite sonicates contained significant levels of aspartate-2-oxoglutarate transaminase (AST; EC 2.6.1.1) and alanine-2- ...
To analyse the cytotoxic effects of KPF on Giardia duodenalis trophozoites and to identify a likely parasite target of this ... The ultrastructure of the trophozoites was analysed by transmission electron microscopy (TEM) whilst apoptosis/necrosis, ... KPF exerted a proapoptotic effect on G. duodenalis trophozoites involving partial interruption of DNA synthesis without ... Ultrastructural and proapoptotic-like effects of kaempferol in Giardia duodenalis trophozoites and bioinformatics prediction of ...
Giardia lamblia GS strain trophozoite antigen/GS Ag (20ug/mL) or WB6 strain trophozoite antigen/WB6 Ag (20ug/mL). The cells ... Giardia trophozoites are typically non-invasive and preferentially colonize the upper small intestinal tract, where they attach ... In vitro stimulation of splenocytes with Giardia lamblia trophozoite antigen. For assessment of parasite-specific IL-17A ... Heyworth, M. F. & Pappo, J. Recognition of a 30,000 MW antigen of Giardia muris trophozoites by intestinal IgA from Giardia- ...
Țara noastră, printre popoarele care vor atinge. Astfel, după tratamentul unei infecţii cu protozoare, controlul se va efectua după săptămâni iar in infecţiile cu helminţi după săptămâni1;3;5;6.
Il est intéressant de noter que pour la première fois des trophozoites de G. muris ont été détectés dans les vaisseaux sanguins ... Certains de ces trophozoites ont été détectés au contact des vaisseaux sanguins et lymphatiques à lintérieur des villosités ... Linvasion des tissus profonds par des trophozoites de Giardia et la présence de ceux-ci au niveau des vaisseaux ouvre sur des ... Some of these trophozoites were detected within central lacteal of damaged villi and nearby blood vessels. Interestingly, and ...
Trophozoites viability under CAD medium. Pyruvate protects Giardia trophozoites from the toxicity induced CAD medium. ... 2). The trophozoites previously incubated with pyruvate (2 mM) and (5 mM) protects trophozoites significantly at 87.1% and 89.3 ... After 8 hr incubation, trophozoites were washed with PBS and added H2DCFDA (1.5 μM). The H2DCFDA loaded trophozoites were ... Pyruvate decreases the level of ROS in Giardia lamblia trophozoites under cysteine-ascorbate deprivation. Giardia trophozoites ...
The aim of the present study was to determine whether the complement-regulatory protein CD59 is present on trophozoites of E. ... Our results show that a CD59-like molecule is expressed in trophozoites of E. histolytica found in colonic amebic lesions. Also ... it has not been proved if CD59 molecules are expressed in the surface during the trophozoites tissue invasion. ... In vitro studies have proved the presence of epitopes of CD59 in the surface of trophozoites of Entamoeba histolytica (E. ...
The trophozoite is conical in shape. Two types of schizogony occur in this family. In the first type the schizonts divide into ... These develop into trophozoites. In the second form and more common form, the schizonts divide into merozoites with large ...
P. falciparum Culture and Trophozoite Isolation. Mycoplasma-free blood-stage parasites of P. falciparum (strain 3D7) were ... For instance, while mature trophozoite-stage P. falciparum parasites are typically sequestered in the microvasculature (to ... Trophozoite stage parasites were isolated by density gradient centrifugation following layering onto a gradient of 40/60/80% ... with non-infected control erythrocytes or trophozoite-stage iRBCs. An iRBC:PBMC ratio of 2:1 was chosen (Scholzen et al., 2009 ...
Oregano kills Giardia intestinalis trophozoites in vitro.May 01, 2006. Click here to read the entire abstract. ...
Unscramble letters trophozoites, Point value for trophozoites, Word Decoder for trophozoites, Word generator using the letters ... trophozoites, Word Solver trophozoites, Possible Scrabble words with trophozoites, Anagram of trophozoites ... Definition of "trophozoites" from Dictionary.com Words that start with trophozoites Words that end with trophozoites Words that ... Scrabble point value for trophozoites: 26 points. Trophozoites is a Words with Friends word. Words with Friends point value for ...
  • A trophozoite (G. trope, nourishment + zoon, animal) is the activated, feeding stage in the life cycle of certain protozoa such as malaria-causing Plasmodium falciparum and those of the Giardia group. (wikipedia.org)
  • The trophozoite life stage of Giardia colonizes and proliferates in the small intestine. (wikipedia.org)
  • The proteinase activity of Giardia lamblia trophozoites, Portland 1 strain, was characterized with respect to substrate specificities and inhibitor sensitivities. (nih.gov)
  • Giardia muris has been maintained for years in the mouse by administering by gavage 10,000 trophozoites taken from the small intestine of infected mice. (ajtmh.org)
  • This paper reports the adaptation of the OFM for imaging Giardia lamblia trophozoites and cysts, a disease-causing parasite species that is commonly found in poor-quality water sources. (caltech.edu)
  • Finally, we report the successful microscopy imaging of both Giardia trophozoites and cysts with an OFM that has a focal plane resolution of 0.8 microns. (caltech.edu)
  • Intact trophozoites of Giardia duodenalis (clone P1C10) took up and metabolised L-[U14C-] aspartate to 14CO2 at rates of 10.27 ± 0.76 and 27.6 ± 2.07 ng hr-1 10-6 cells in a simple maintenance medium (MM) and in a complex bile supplemented (BIS-33) medium respectively. (edu.au)
  • The extrusion or uptake of alanine by Giardia trophozoites may be dictated by the intracellular redox-status of the protozoan parasite or components in the external mileu. (edu.au)
  • Ultrastructural and proapoptotic-like effects of kaempferol in Giardia duodenalis trophozoites and bioinformatics prediction of its potential protein target. (bvsalud.org)
  • To analyse the cytotoxic effects of KPF on Giardia duodenalis trophozoites and to identify a likely parasite target of this compound. (bvsalud.org)
  • During routine transmission electron microscopic (TEM) examination of mice naturally infected with Giardia muris , an intense infection with Giardia trophozoites was demonstrated within intestinal and renal tissues. (parasite-journal.org)
  • Duodenal sections were found loaded with Giardia trophozoites in intimate contact with necrotic gut cells. (parasite-journal.org)
  • Involvement of deep tissues by Giardia trophozoites and their presence within vascular channels could open up questions about the possible invasive and disseminative behavior of G. muris, particularly in heavily and naturally infected hosts. (parasite-journal.org)
  • À l'occasion d'un examen en microscopie électronique de souris naturellement infectées par Giardia muris , des trophozoites de Giardia en très grand nombre ont été observés au niveau des tissus intestinaux et rénaux. (parasite-journal.org)
  • L'invasion des tissus profonds par des trophozoites de Giardia et la présence de ceux-ci au niveau des vaisseaux ouvre sur des questions au sujet des modalités d'invasion et de dissémination de G. muris , particulièrement chez des hôtes naturellement fortement parasités. (parasite-journal.org)
  • Pyruvate recovers Giardia trophozoites from oxidative stress by decreasing the number of DNA breaks that might favor DNA repair. (parasitol.kr)
  • The trophozoites of Giardia are also protected by ascorbic acid under high partial pressure of oxygen [ 11 ]. (parasitol.kr)
  • Compared with many protists, Giardia lamblia has a simple life cycle alternating between cyst and trophozoite. (biomedcentral.com)
  • Most research on the molecular biology of Giardia parasites has focused on trophozoites and the processes of excystation and encystation, whereas cysts have attracted less interest. (biomedcentral.com)
  • We have examined by gelatin - SDS-PAGE the protease activity in cell lysates of Giardia duodenalis trophozoites of two axenic strains isolated in Brazil from a symptomatic patient (BTU-11) and an asymptomatic carrier (BTU-10), and the reference strain Portland 1 (P1). (bvsalud.org)
  • Alterations on growth and cell organization of Giardia intestinalis trophozoites after treatment with KH-TFMDI, a novel class III histone deacetylase inhibitor. (ox.ac.uk)
  • Giardia trophozoites have developed resistance mechanisms to currently available compounds, leading to treatment failures. (ox.ac.uk)
  • Detection of secreted Giardia proteins during human cell-trophozoite interactions in vitro . (nih.gov)
  • The presence of ADI, enolase and OCT was detected in concentrated culture supernatants from co-cultures of Giardia lamblia WB-C6 and differentiated Caco-2 human intestinal epithelial cells and to lesser extent G. lamblia trophozoites alone (A). The ADI secretion increases 7-fold within the first 15 min upon interaction, while enolase increases 3-fold. (nih.gov)
  • The traditional basis of diagnosis is identification of Giardia intestinalis trophozoites or cysts in the stool of infected patients via a stool ova and parasite (O&P) examination. (medscape.com)
  • cysts and trophozoites (31/70), and Giardia sp. (nih.gov)
  • Nitazoxanide inhibits the growth of Cryptosporidium parvum and Giardia lamblia trophozoites. (medscape.com)
  • Cytopathogenicity of Entamoeba histolytica: hemolytic activity of trophozoite homogenates. (biomedsearch.com)
  • Upon exposure to Entamoeba histolytica antigen, lymphocytes from patients treated for amebic liver abscess produce lymphokines which activate monocyte-derived macrophages to kill E. histolytica trophozoites. (ajtmh.org)
  • In vitro studies have proved the presence of epitopes of CD59 in the surface of trophozoites of Entamoeba histolytica (E. histolytica). (semanticscholar.org)
  • Entamoeba histolytica: adhesins and lectins in the trophozoite surface. (semanticscholar.org)
  • Early interactions of Entamoeba histolytica trophozoites with parenchymal and inflammatory cells in the hamster liver: an immunocytochemical study. (semanticscholar.org)
  • Feb 07, 2021 · Entamoeba coli has 'three different morphological forms in each life cycle: trophozoite, precystic stage and cystic stage. (yahoo.com)
  • Intraperitoneal inoculation of axenically cultured Entamoeba histolytica trophozoites constitutes an easy to perform, highly reproducible procedure for inducing amebic liver abscesses in hamsters. (elsevierpure.com)
  • 63/74), Entamoeba hartmanni cysts and trophozoites (19/70), Endolimax nana cysts (31/70), Iodamoeba buetschlii cysts (11/70), Endolimax nana or Iodamoeba buetschlii trophozoites (63/70). (nih.gov)
  • Entamoeba coli cysts and trophozoites (14/70), Entamoeba histolytica trophozoite (1/70), Chilomastix sp. (nih.gov)
  • Do Entamoeba histolytica trophozoites signal via enteric microvilli? (ugent.be)
  • Disturbance of tight junctions by Entamoeba histolytica: Resistant vertebrate cell types and incompetent trophozoites. (ugent.be)
  • In summary, IFN-γ has a major but not exclusive role in activating human monocyte-derived macrophages in vitro to kill virulent E. histolytica trophozoites. (ajtmh.org)
  • MTT assays in trophozoite cultures of E. histolytica exposed to each peptide (1-1000 μM) showed that Lfampin is far more amoebicidal than Lfcins. (portlandpress.com)
  • The aim of the present study was to determine whether the complement-regulatory protein CD59 is present on trophozoites of E. histolytica in human colon. (semanticscholar.org)
  • Our results show that a CD59-like molecule is expressed in trophozoites of E. histolytica found in colonic amebic lesions. (semanticscholar.org)
  • Based on the present morphological time-sequence study, we suggest that inflammatory cells associated with E. histolytica trophozoites play an important role in commencing the damage of liver sheaths and producing the subsequent parenchymal lesions. (elsevierpure.com)
  • The extraordinary phagocytic activity of E. histolytica trophozoites has been accepted as one of the virulence mechanisms responsible for their invasive capacity. (hindawi.com)
  • E. histolytica invasion starts when trophozoites residing in the colon deplete the mucus, interact with enterocytes, dismantle cell junctions, and lyse host cells [ 6 ], whereas E. dispar does not break down the mucus barrier or cause epithelial cell damage when in contact with cells on human colonic explants [ 7 ]. (hindawi.com)
  • In 90% of the infected patients, E. histolytica trophozoites normally inhabit the colon and spend their time in the host as a non-pathogenic commensal. (frontiersin.org)
  • Isolation of Plasmodium falciparum by flow-cytometry: implications for single-trophozoite genotyping and parasite DNA purification for whole-genome high-throughput sequencing of archival samples. (inserm.fr)
  • For trophozoite isolation, parasite and host nuclei are stained using propidium iodide (PI) followed by flow cytometry and cell sorting to separate trophozoites from host cells. (inserm.fr)
  • The investigation entails the construction and validation of a detailed mathematical model for glycolysis of the malaria parasite Plasmodium falciparum in the blood stage trophozoite form. (fairdomhub.org)
  • The striking morphological differences between the dormant cyst and the rapidly dividing and motile trophozoite implies profound changes in the metabolism as the parasite encysts in the host's intestine and excysts upon ingestion by a new host. (biomedcentral.com)
  • These cells form a rim of polymorphonuclear leukocytes surrounding the amebas, which adhere to the trophozoite and can sometimes be observed polarized to one end of the parasite, suggesting capping of surface receptors. (elsevierpure.com)
  • The motile form of the parasite, the trophozoite, usually lives as a harmless commensal in the lumen of the large intestine where it multiplies and differentiates into a cyst, the resistance form, responsible for transmission of the infection. (hindawi.com)
  • Once released within the intestine, the trophozoites multiply by feeding on intestinal bacteria or by invading the lining of the large intestine . (encyclopedia.com)
  • Within the lining of the large intestine, the trophozoites secrete a substance that destroys intestinal tissue and creates sores (ulcers) or abscesses. (encyclopedia.com)
  • Trophozoites may carpet the intestinal villi. (vetstream.com)
  • Occasionally, trophozoites would invade the intestinal mucosa and produce dysentery or amoeba and spread to other organs [ 3 ]. (hindawi.com)
  • Intestinal protozoa are transmitted by the fecal-oral route and tend to exhibit similar life cycles consisting of a cyst stage and a trophozoite stage (Figure). (tulane.edu)
  • METHODS: The method described allows isolation of Plasmodium falciparum trophozoites, genotyping and whole genome sequencing from the blood of infected patients. (inserm.fr)
  • A large scale Plasmodium vivax- Saimiri boliviensis trophozoite-schizont transition proteome. (cdc.gov)
  • Intact trophozoite of G. duodenalis (clone P1C10) also took up and metabolised L-[U14C-] alanine to 14CO2 at rates of 20.6 ± 1.1 and 91.4 ± 17.5 ng hr-1 10-6 cells in the simple (MM) and complex (BIS-33) medium respectively. (edu.au)
  • KPF exerted a proapoptotic effect on G. duodenalis trophozoites involving partial interruption of DNA synthesis without oxidative stress or structure damage to chromatin and cytoskeletal structures. (bvsalud.org)
  • In general, the results revealed protease activities in G. duodenalis trophozoites of Brazilian axenic strains and the predominance of cysteine proteinases . (bvsalud.org)
  • Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch & C.A. Mey) and Inula oculus - christi (L.) on Acanthamoeba castellanii cysts and trophozoites. (springer.com)
  • In vitro amoebicidal activity of Origanum syriacum and Origanumla evigatum on Acanthamoeba castellanii cysts and trophozoites. (springer.com)
  • Light and transmission electron microscopy observations are reported on the structure and in vitro cytopathic effect of Acanthamoeba griffini trophozoites isolated from a clinical case. (hindawi.com)
  • In in vitro assays the hemolytic activity of total homogenates of trophozoites of seven strains of amebae were tested for specificity and potency against the erythrocytes of eight mammalian species. (biomedsearch.com)
  • Research on G. lamblia is aided by the fact that the entire life cycle can be studied outside the host, and that the differentiation from cyst to trophozoite and the reverse process of encystation can be reproduced in vitro. (biomedcentral.com)
  • In this study, we observed the cytopathic effect, in vitro cytotoxicity, and secretion pattern of cytokines in human corneal epithelial cells (HCECs) induced by A. castellanii trophozoites and/or cysts . (bvsalud.org)
  • An external ventricular drain was placed because of clinical instability, and CSF microscopy showed motile trophozoites on a wet preparation and Giemsa stain, consistent with N. fowleri . (mja.com.au)
  • After ingestion by an appropriate host, the cysts transform into trophozoites which exhibit an active metabolism and are usually motile. (tulane.edu)
  • Specifically, a diagnosis of balantidiasis is made by finding B. coli cysts or trophozoites in the patient's stools or by finding trophozoites in tissue samples (biopsy) taken from the large bowel. (encyclopedia.com)
  • The secreted G. lamblia proteins were localized to the cytoplasm and the inside of the plasma membrane of trophozoites. (nih.gov)
  • Here, we use live-cell imaging and photobleaching to monitor the uptake of the pH-sensitive fluorescent tracer SNARF-1-dextran from the erythrocyte cytoplasm in ring-stage and trophozoite-stage parasites. (biologists.org)
  • Rounded, compact Dark 8, merozoites (6 to merozoites trophozoite with brown, "rosette"P. malariae Mature normal Zeimanns 12) giving a coarse released dense cytoplasm/ rounded cons- schizonts granular every 36- band form troph are picuous are appearance. (slideshare.net)
  • In the apicomplexan life cycle the trophozoite undergoes schizogony (asexual reproduction) and develops into a schizont which contains merozoites. (wikipedia.org)
  • Irregular, ameboid Golden larger than uninfected red of this species (16- 12-24 merozoites in trophozoite, has brown, P.vivax Young enlarge Schauffners blood cells. (slideshare.net)
  • Trophozoites develop during the course of the infection into cysts which is the infectious life stage. (wikipedia.org)
  • Using a recently published P. vivax reference genome, we report the proteome from two biological replicates of infected Saimiri boliviensis host reticulocytes undergoing transition from the late trophozoite to early schizont stages. (cdc.gov)
  • Functional categories of P . vivax and S . boliviensis trophozoite-schizont transition iRBC proteins. (cdc.gov)
  • To investigate the magnitude of the transcriptional changes occurring during the G. lamblia life cycle we compared the transcriptome of G. lamblia trophozoites and cysts using single-color oligonucleotide microarrays. (biomedcentral.com)
  • The comparison of the G. lamblia cyst and trophozoite transcriptome showed that transcripts of most genes are present at a lower level in cysts. (biomedcentral.com)
  • Localization of identified proteins in G. lamblia trophozoites by polyclonal mouse antibodies (green) raised against purified recombinant ADI (A), OCT (B) and enolase (C). Corresponding light microscopy pictures are shown in panels (D-F). Figs. (G-I) shows the localization of ADI (G), OCT (H) and enolase (I) during interaction with differentiated Caco-2 cells. (nih.gov)
  • Trophozoites viability under cysteine-ascorbate deprived (CAD) medium in presence and absence of extracellular pyruvate has also been measured. (parasitol.kr)
  • Interestingly, and for the first time to be demonstrated, morphologically identical G. muris trophozoite was detected in a renal blood vessel. (parasite-journal.org)
  • Il est intéressant de noter que pour la première fois des trophozoites de G. muris ont été détectés dans les vaisseaux sanguins rénaux. (parasite-journal.org)
  • In the present study we evaluated the efficacies of various biocides against trophozoites and cysts of several Acanthamoeba strains. (asm.org)
  • RESULTS AND CONCLUSION: The results demonstrate that single trophozoite genotyping is possible and that cell sorting can be successfully applied to reduce the contaminating host DNA for subsequent whole genome sequencing of parasites extracted from infected blood samples. (inserm.fr)
  • However, it has not been proved if CD59 molecules are expressed in the surface during the trophozoites' tissue invasion. (semanticscholar.org)
  • Trophozoites infect people and animals by penetrating the nasal tissue and migrating to the brain through the olfactory nerves, causing primary amoebic meningoencephalitis or, 'PAM. (disabled-world.com)
  • Naegleria fowleri trophozoites are found in cerebrospinal fluid (CSF) and tissue, while flagellated forms are occasionally found in CSF. (disabled-world.com)
  • These organisms have the same life cycle (Figure 262-1), characterized by a trophozoite stage, which lives in the colon, and a cyst stage, which is infectious. (yahoo.com)
  • A number of host and pathogen proteins contained highly oxidized or nitrated residues, extending prior trophozoite-enriched stage observations from S. boliviensis infections, and supporting the possibility of oxidative stress in relation to the disease. (cdc.gov)
  • The cyst and trophozoite transcriptome were compared by plotting mean Cy3 fluorescence values from six replicate microarrays hybridized with cDNA from independent live cyst suspensions and two replicate microarrays hybridized with trophozoite cDNA. (biomedcentral.com)
  • Trophozoites replicate by binary division during which the nuclear membrane remains intact. (disabled-world.com)
  • The nucleus goes through dramatic changes from multiple spheres of condensed chromatin in unassociated trophozoites, to mostly uncondensed chromatin in paired gamonts, to a large single sphere of condensed chromatin encasing many smaller spheres in gamonts in syzygy. (bioone.org)
  • every 72 chromatin mass of multiple trophozoites gametocyte hrs in one cell. (slideshare.net)
  • Three distinct morphological forms exist airing the life cycle-Trophozoite, Pre-cystic stage and Cystic stage. (yahoo.com)
  • These MAbs reacted with the three morphological forms of N. fowleri (trophozoites, cysts, and flagellates). (coventry.ac.uk)
  • Here, we describe the ultrastructure of the syzygy junction and the nucleus during the transition from unassociated trophozoites to paired gamonts to gamonts in syzygy. (bioone.org)
  • Marc A. Toso and Charlotte K. Omoto "ULTRASTRUCTURE OF THE GREGARINA NIPHANDRODES NUCLEUS THROUGH STAGES FROM UNASSOCIATED TROPHOZOITES TO GAMONTS IN SYZYGY AND THE SYZYGY JUNCTION," Journal of Parasitology 93(3), 479-484, (1 June 2007). (bioone.org)
  • However, the reasons why these trophozoites become virulent and invasive are unknown. (frontiersin.org)
  • Thin smears were prepared from Percoll-gradient enriched iRBC, and show early nucleated schizonts (iRBC 1, 4 and 5) and large late-stage trophozoites (iRBC 2 and 3). (cdc.gov)
  • most of the time, the active (feeding) stage is termed a "trophozoite. (k-state.edu)
  • During the first stage, the vegetative trophozoite form is feeding and replicating. (asm.org)
  • The second stage develops not only under unfavorable environmental conditions, such as nutrient starvation, heat, cold, and desiccation, but also in the presence of elevated concentration of various chemical compounds, with the trophozoites differentiating into cysts ( 13 , 40 ). (asm.org)
  • Trophozoites can turn into a temporary and non-feeding flagellated stage when stimulated by adverse environmental changes, such as a reduced food source. (disabled-world.com)
  • They revert back to the trophozoite stage when favorable conditions return. (disabled-world.com)
  • Trophozoite and cyst stages are shown in the life cycle of Balantidium coli the causative agent of balantidiasis. (wikipedia.org)
  • Here, we evaluated the activity of KH-TFMDI, a novel 3-arylideneindolin-2-one-type sirtuin inhibitor, on G. intestinalis trophozoites. (ox.ac.uk)
  • Taken together, these data show that KH-TFMDI has significant effects against G. intestinalis trophozoites proliferation and structural organization and suggest that histone deacetylation pathway should be explored on this protozoon as target for chemotherapy. (ox.ac.uk)
  • In the digestive tract, the cysts are transported to the intestine where the walls of the cysts are broken open by digestive secretions, releasing the mobile trophozoites. (encyclopedia.com)
  • An intense cellular immune reaction was obviously demonstrated with remarkable interaction between giant macrophages and the trophozoites particulates. (parasite-journal.org)
  • The ultrastructure of the trophozoites was analysed by transmission electron microscopy (TEM) whilst apoptosis / necrosis , production of reactive oxygen species (ROS) and cell cycle progression were assessed by flow cytometry (FCM) and confocal laser microscopy (CLM). (bvsalud.org)
  • Light and electron microscopy analysis showed the presence of multinucleated cell clusters suggesting that the cytokinesis could be compromised in treated trophozoites. (ox.ac.uk)
  • and the disease-producing form, the trophozoite, which although capable of moving, cannot survive once excreted in the feces and, therefore, cannot infect others. (encyclopedia.com)
  • In the presence of methanolic extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellani trophozoites and cysts were decreased during the experimental process. (cumhuriyet.edu.tr)
  • Both extracts showed time- and dose-dependent amoebicidal action on the trophozoites and cysts. (cumhuriyet.edu.tr)
  • Among the extracts tested, T. chamaedrys showed the strongest amoebicidal effect on the trophozoites. (cumhuriyet.edu.tr)
  • As expected, cysts were found more resistant to the extracts than the trophozoites. (cumhuriyet.edu.tr)
  • Some of these trophozoites were detected within central lacteal of damaged villi and nearby blood vessels. (parasite-journal.org)
  • This global view of the cyst and trophozoite transcriptome complements studies focused on the expression of selected genes during trophozoite multiplication, encystation and excystation. (biomedcentral.com)
  • The present study aimed to investigate the amoebicidal and amoebistatic efects of Artemisia argyi leaf methanolic extract by testing the effects on trophozoites and on cysts. (springer.com)
  • In 100 μl culture, different concentrations of A. argyi methanolic extract (in quantities from 1.2, 2.3, 4.7, 9.4, 18.7, 37.4, 74.8 mg/ml) and the same volume of trophozoite/cyst suspension were mixed for the determination of the amoebicidal activity of the plant extract. (springer.com)
  • The growth of trophozoites stopped in A. argyi methanolic extract with 50% inhibitory concentrations (IC50)/8 h for 37.4 mg/ ml and 74.8 mg/ml extract solution and had stronger amoebicidal activity on the cysts with IC50/72 h. (springer.com)
  • These different types can be distinguished by the overall shape and dimensions of the trophozoite body and by the distinctive shapes of the median bodies. (lww.com)
  • In the presence of 16 mg/ml or above extract concentrations, no viable trophozoites were observed within 48 h. (cumhuriyet.edu.tr)
  • Une réaction cellulaire intense a été observée avec d'importantes interactions entre cellules macrophagiques géantes et trophozoites. (parasite-journal.org)
  • In the case of T. polium, no viable trophozoites were observed within 48 h at 32 mg/ml concentration. (cumhuriyet.edu.tr)
  • Trophozoites were inactivated by all treatments except those using glutaraldehyde as an active compound: for these treatments, we observed resistance even after 30 min exposure. (asm.org)
  • The complement of the trophozoite state is the thick-walled cyst form). (wikipedia.org)