Transformation, Bacterial: The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.Altitude: A vertical distance measured from a known level on the surface of a planet or other celestial body.Love: Affection; in psychiatry commonly refers to pleasure, particularly as it applies to gratifying experiences between individuals.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Cell Transformation, Viral: An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.Ice: The solid substance formed by the FREEZING of water.Pollen Tube: A growth from a pollen grain down into the flower style which allows two sperm to pass, one to the ovum within the ovule, and the other to the central cell of the ovule to produce endosperm of SEEDS.Biotechnology: Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.Intubation, Gastrointestinal: The insertion of a tube into the stomach, intestines, or other portion of the gastrointestinal tract to allow for the passage of food products, etc.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Ice Cover: A thick mass of ICE formed over large regions of land; RIVERS; LAKES; ponds; or SEAWATER.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Papaver: A genus of Eurasian herbaceous plants, the poppies (family PAPAVERACEAE of the dicotyledon class Magnoliopsida), that yield OPIUM from the latex of the unripe seed pods.Social Work: The use of community resources, individual case work, or group work to promote the adaptive capacities of individuals in relation to their social and economic environments. It includes social service agencies.Superior Mesenteric Artery Syndrome: DUODENAL OBSTRUCTION by the superior mesenteric artery (MESENTERIC ARTERY, SUPERIOR) which travels in the root of the MESENTERY and crosses over the DUODENUM. The syndrome is characterized by the dilated proximal duodenum and STOMACH, bloating, ABDOMINAL CRAMPS, and VOMITING. Often it is observed in patient with body casts after spinal surgery.LondonAnimal Welfare: The protection of animals in laboratories or other specific environments by promoting their health through better nutrition, housing, and care.Animals, LaboratoryEvidence-Based Nursing: A way of providing nursing care that is guided by the integration of the best available scientific knowledge with nursing expertise. This approach requires nurses to critically assess relevant scientific data or research evidence, and to implement high-quality interventions for their nursing practice.Nursing Research: Research carried out by nurses, generally in clinical settings, in the areas of clinical practice, evaluation, nursing education, nursing administration, and methodology.Biomedical Research: Research that involves the application of the natural sciences, especially biology and physiology, to medicine.Electroporation: A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.Rickettsiaceae: A family of small, gram-negative organisms, often parasitic in humans and other animals, causing diseases that may be transmitted by invertebrate vectors.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Agrobacterium: A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Bacillus subtilis: A species of gram-positive bacteria that is a common soil and water saprophyte.Foreign Bodies: Inanimate objects that become enclosed in the body.Desulfovibrio desulfuricans: The type species of gram-negative, anaerobic bacteria of the genus DESULFOVIBRIO. It is found in FRESHWATER; SOIL, and in marine or brackish water.Technical ReportProkaryotic Cells: Cells lacking a nuclear membrane so that the nuclear material is either scattered in the cytoplasm or collected in a nucleoid region.Methylmercury Compounds: Organic compounds in which mercury is attached to a methyl group.Mercury: A silver metallic element that exists as a liquid at room temperature. It has the atomic symbol Hg (from hydrargyrum, liquid silver), atomic number 80, and atomic weight 200.59. Mercury is used in many industrial applications and its salts have been employed therapeutically as purgatives, antisyphilitics, disinfectants, and astringents. It can be absorbed through the skin and mucous membranes which leads to MERCURY POISONING. Because of its toxicity, the clinical use of mercury and mercurials is diminishing.Health ResortsNuclear Weapons: A weapon that derives its destructive force from nuclear fission and/or fusion.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Nanotechnology: The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.Mercury PoisoningSarcoma, YoshidaNanotubes, Carbon: Nanometer-sized tubes composed mainly of CARBON. Such nanotubes are used as probes for high-resolution structural and chemical imaging of biomolecules with ATOMIC FORCE MICROSCOPY.Magnesium Silicates: A generic term for a variety of compounds that contain silicon, oxygen, and magnesium, and may contain hydrogen. Examples include TALC and some kinds of ASBESTOS.Calcium Chloride: A salt used to replenish calcium levels, as an acid-producing diuretic, and as an antidote for magnesium poisoning.Nanostructures: Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.

RecA-Mediated gene conversion and aminoglycoside resistance in strains heterozygous for rRNA. (1/2249)

Clinical resistance to aminoglycosides in general is due to enzymatic drug modification. Mutational alterations of the small ribosomal subunit rRNA have recently been found to mediate acquired resistance in bacterial pathogens in vivo. In this study we investigated the effect of 16S rRNA heterozygosity (wild-type [wt] and mutant [mut] operons at position 1408 [1408wt/1408mut]) on aminoglycoside resistance. Using an integrative vector, we introduced a single copy of a mutated rRNA operon (1408 A-->G) into Mycobacterium smegmatis, which carries two chromosomal wild-type rRNA operons; the resultant transformants exhibited an aminoglycoside-sensitive phenotype. In contrast, introduction of the mutated rRNA operon into an M. smegmatis rrnB knockout strain carrying a single functional chromosomal wild-type rRNA operon resulted in aminoglycoside-resistant transformants. Subsequent analysis by DNA sequencing and RNase protection assays unexpectedly demonstrated a homozygous mutant genotype, rRNAmut/rRNAmut, in the resistant transformants. To investigate whether RecA-mediated gene conversion was responsible for the aminoglycoside-resistant phenotype in the rRNAwt/rRNAmut strains, recA mutant strains were generated by allelic exchange techniques. Transformation of the recA rrnB M. smegmatis mutant strains with an integrative vector expressing a mutated rRNA operon (Escherichia coli position 1408 A-->G) resulted in transformants with an aminoglycoside-sensitive phenotype. Subsequent analysis showed stable heterozygosity at 16S rRNA position 1408 with a single wild-type allele and a single resistant allele. These results demonstrate that rRNA-mediated mutational resistance to aminoglycosides is recessive.  (+info)

Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide. (2/2249)

Pyrazinamide (PZA), an analog of nicotinamide, is a prodrug that requires conversion to the bactericidal compound pyrazinoic acid (POA) by the bacterial pyrazinamidase (PZase) activity of nicotinamidase to show activity against Mycobacterium tuberculosis. Mutations leading to a loss of PZase activity cause PZA resistance in M. tuberculosis. M. kansasii is naturally resistant to PZA and has reduced PZase activity along with an apparently detectable nicotinamidase activity. The role of the reduction in PZase activity in the natural PZA resistance of M. kansasii is unknown. The MICs of PZA and POA for M. kansasii were determined to be 500 and 125 micrograms/ml, respectively. Using [14C]PZA and [14C]nicotinamide, we found that M. kansasii had about 5-fold-less PZase activity and about 25-fold-less nicotinamidase activity than M. tuberculosis. The M. kansasii pncA gene was cloned on a 1.8-kb BamHI DNA fragment, using M. avium pncA probe. Sequence analysis showed that the M. kansasii pncA gene encoded a protein with homology to its counterparts from M. tuberculosis (69.9%), M. avium (65.6%), and Escherichia coli (28.5%). Transformation of naturally PZA-resistant M. bovis BCG with M. kansasii pncA conferred partial PZA susceptibility. Transformation of M. kansasii with M. avium pncA caused functional expression of PZase and high-level susceptibility to PZA, indicating that the natural PZA resistance in M. kansasii results from a reduced PZase activity. Like M. tuberculosis, M. kansasii accumulated POA in the cells at an acidic pH; however, due to its highly active POA efflux pump, the naturally PZA-resistant species M. smegmatis did not. These findings suggest the existence of a weak POA efflux mechanism in M. kansasii.  (+info)

Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili. (3/2249)

We have recently described the expression of two pili of different lengths on the surface of Legionella pneumophila (B. J. Stone and Y. Abu Kwaik, Infect. Immun. 66:1768-1775, 1998). Production of long pili requires a functional pilEL locus, encoding a type IV pilin protein. Since type IV pili in Neisseria gonorrhoeae are associated with competence for DNA transformation, we examined the competence of L. pneumophila for DNA transformation under conditions that allowed the expression of type IV pili. We show that L. pneumophila is naturally competent for DNA transformation by isogenic chromosomal DNA and by plasmid DNA containing L. pneumophila DNA. Many different L. pneumophila loci are able to transform L. pneumophila after addition of plasmid DNA, including gspA, ppa, asd, and pilEL. The transformation frequency is reduced when competing DNA containing either L. pneumophila DNA or vector sequences is added to the bacteria, suggesting that uptake-specific sequences may not be involved in DNA uptake. Competence for DNA transformation correlates with expression of the type IV pili, and a pilEL mutant defective in expression of type IV pili is not competent for DNA transformation. Complementation of the mutant for competence is restored by the reintroduction of a cosmid that restores production of type IV pili. Minimal competence is restored to the mutant by introduction of pilEL alone. We conclude that competence for DNA transformation in L. pneumophila is associated with expression of the type IV pilus and results in recombination of L. pneumophila DNA into the chromosome. Since expression of type IV pili also facilitates attachment of L. pneumophila to mammalian cells and protozoa, we designated the type IV pili CAP (for competence- and adherence-associated pili).  (+info)

Metabolic engineering of a 1,2-propanediol pathway in Escherichia coli. (4/2249)

1,2-Propanediol (1,2-PD) is a major commodity chemical that is currently derived from propylene, a nonrenewable resource. A goal of our research is to develop fermentation routes to 1,2-PD from renewable resources. Here we report the production of enantiomerically pure R-1,2-PD from glucose in Escherichia coli expressing NADH-linked glycerol dehydrogenase genes (E. coli gldA or Klebsiella pneumoniae dhaD). We also show that E. coli overexpressing the E. coli methylglyoxal synthase gene (mgs) produced 1,2-PD. The expression of either glycerol dehydrogenase or methylglyoxal synthase resulted in the anaerobic production of approximately 0.25 g of 1,2-PD per liter. R-1,2-PD production was further improved to 0.7 g of 1,2-PD per liter when methylglyoxal synthase and glycerol dehydrogenase (gldA) were coexpressed. In vitro studies indicated that the route to R-1,2-PD involved the reduction of methylglyoxal to R-lactaldehyde by the recombinant glycerol dehydrogenase and the reduction of R-lactaldehyde to R-1, 2-PD by a native E. coli activity. We expect that R-1,2-PD production can be significantly improved through further metabolic and bioprocess engineering.  (+info)

The specific genes for lantibiotic mutacin II biosynthesis in Streptococcus mutans T8 are clustered and can be transferred en bloc. (5/2249)

Mutacin II is a ribosomally synthesized peptide lantibiotic produced by group II Streptococcus mutans. DNA sequencing has revealed that the mutacin II biosynthetic gene cluster consists of seven specific open reading frames: a regulator (mutR), the prepromutacin structural gene (mutA), a modifying protein (mutM), an ABC transporter (mutT), and an immunity cluster (mutFEG). Transformations of a non-mutacin-producing strain, S. mutans UA159, and a mutacin I-producing strain, S. mutans UA140, with chromosomal DNA from S. mutans T8 with an aphIII marker inserted upstream of the mutacin II structural gene yielded transformants producing mutacin II and mutacins I and II, respectively.  (+info)

Bacterial evolution: bacteria play pass the gene. (6/2249)

DNA transfer between related bacterial species is enhanced by species-specific uptake sequences. These sequences have been used to identify genes that have been transferred from Haemophilus to Neisseria, providing a clear example of interspecific transfer of DNA in the evolution of the pathogenic Neisseria.  (+info)

Characterization of the recD gene of Neisseria gonorrhoeae MS11 and the effect of recD inactivation on pilin variation and DNA transformation. (7/2249)

Pilin antigenic variation in Neisseria gonorrhoeae may result following intrachromosomal recombination between homologous pil genes. Despite extensive study, recA is the only previously characterized gene known to be involved in this process. In this study, the gonococcal recD gene, encoding one subunit of the putative RecBCD holoenzyme, was characterized and its role in pilin variation assessed. The complete recD gene of N. gonorrhoeae MS11 was cloned and its nucleotide sequence determined. The gonococcal recD gene complemented a defined Escherichia coli recD mutant, based on plaque formation of bacteriophage lambda and the restoration of ATP-dependent nuclease activity. Inactivation of the gonococcal recD gene had no measurable effect on cell viability or survival following UV exposure, but did decrease the frequency of DNA transformation approximately threefold. The frequency at which non-parental pilin phenotypes were spawned was 12-fold greater in MS11 recD mutants compared with the parental MS11 rec+ strain. Similar results were obtained using recD mutants that were not competent for DNA transformation. Complementation of the MS11 recD mutant with a wild-type recD gene copy restored the frequency of pilin phenotypic variation to approximately wild-type levels. The nucleotide changes at pilE in the recD mutants were confined to the variable regions of the gene and were similar to changes previously attributed to gene conversion.  (+info)

Identification of Haemophilus influenzae Rd transformation genes using cassette mutagenesis. (8/2249)

Genes required for natural transformation of Haemophilus influenzae Rd were identified by a cassette mutagenesis protocol consisting of the following steps: random insertional mutagenesis, phenotypic screening, sequencing of genome sequence tags from the DNA flanking the insertion in the selected mutants and comparison of genome sequence tags to genomic sequence data. The cassette mutagenesis screen for transformation genes resulted in five distinct mutant classes, two of which have been identified in previous studies. Insertions in the three newly identified loci interrupted genes with predicted protein products homologous to a type IV pilin-like protein biogenesis operon, drug-efflux transporters and a phospholipid-biosynthesis enzyme. The most significant finding of this screen is the requirement for type IV pilin-like proteins in genetic transformation of H. influenzae. These surface structures are utilized for DNA uptake in a number of Gram-positive and Gram-negative bacteria, and appear to be the common component among the systems for DNA binding.  (+info)

*Genetics

2000). "Bacterial transformation". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. ... 2000). "Bacterial conjugation". An Introduction to Genetic Analysis (7th ed.). New York: W. H. Freeman. ISBN 0-7167-3520-2. ... Inductions of transformation by a desoxyribonucleic acid fraction isolated from pneumococcus type III". The Journal of ... Biology portal Bacterial genome size Cryoconservation of animal genetic resources Eugenics Embryology Evolution Genetic ...

*PBLU

"BIOTECHNOLOGY: BACTERIAL TRANSFORMATION" (PDF). CollegeBoard. Retrieved 29 October 2017. ... pBLU is a commercially produced bacterial plasmid that contains genes for ampicillin resistance (beta lactamase and beta ... coli strain to teach students about transformation of eubacteria. It is 5,437 base pairs long. There is a multiple cloning site ...

*Vibrio

Natural transformation is a common bacterial adaptation for DNA transfer that employs numerous bacterial gene products. For a ... 496-8. ISBN 0-387-24144-2. Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): ... Natural transformation has also been described for V. fisheri, V. vulnificus and V. parahaemolyticus. V. cholerae has been used ... However, Vibrio Müller, 1773 became regarded as the name of a zoological genus, and the name of the bacterial genus became ...

*Microbial genetics

Natural transformation is a bacterial adaptation for DNA transfer between two cells through the intervening medium. The uptake ... Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge ... Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nature Reviews Microbiology. 2 (3): 241-9. doi:10.1038/ ... "Studies on the chemical nature of the substance inducing transformation of pneumococcal types. Inductions of transformation by ...

*Genetic engineering

Chen, I; Dubnau, D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-9. doi:10.1038/ ... Due to the damage caused to the cells and DNA the transformation efficiency of biolistics and electroporation is lower than ... National Academies Press (US). Gelvin, S. B. (2003). "Agrobacterium-Mediated Plant Transformation: The Biology behind the "Gene ... "Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome". Science. 329 (5987): 52-6. doi:10.1126/science. ...

*Mating system

Transformation appears to be common among bacterial species, and at least 60 species are known to have the natural ability to ... Transformation, unlike transduction or conjugation, depends on numerous bacterial gene products that specifically interact to ... This response appears to be a primitive form of sexual interaction similar to the more well-studied bacterial transformation ... Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-9. doi:10.1038/ ...

*Homologous recombination

Transformation, unlike bacterial conjugation and transduction, depends on numerous bacterial gene products that specifically ... Thus transformation is clearly a bacterial adaptation for DNA transfer. In order for a bacterium to bind, take up and integrate ... Natural bacterial transformation involves the transfer of DNA from a donor bacterium to a recipient bacterium, where both donor ... The RecA/Rad51/DMC1 gene family plays a central role in homologous recombination during bacterial transformation as it does ...

*Organism

In prokaryotes, natural bacterial transformation involves the transfer of DNA from one bacterium to another and integration of ... Natural bacterial transformation is considered to be a primitive sexual process and occurs in both bacteria and archaea, ... Transformation is clearly a bacterial adaptation and not an accidental occurrence, because it depends on numerous gene products ... Chen I; Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-9. doi:10.1038/ ...

*Genetic engineering techniques

Transformation using electroporation was developed in the late 1980s, increasing the efficiency and bacterial range. In 1907 a ... "DNA uptake during bacterial transformation". Nature Reviews Microbiology. 2 (3): 241-49. doi:10.1038/nrmicro844. PMID 15083159 ... In biolistic transformation, particles of gold or tungsten are coated with DNA and then shot into young plant cells or plant ... Another transformation method for plant and animal cells is electroporation, which involves subjecting cells to an electric ...

*Horizontal gene transfer

Natural transformation is a bacterial adaptation for DNA transfer (HGT) that depends on the expression of numerous bacterial ... Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-9. doi:10.1038/ ... The capacity for natural transformation occurs in at least 67 prokaryotic species. Competence for transformation is typically ... In general, transformation is a complex, energy-requiring developmental process. In order for a bacterium to bind, take up and ...

*Frederick Griffith

Griffith also reported transformation of serological type-bacterial antigenicity-distinct from presence or absence of a capsule ... Ottolenghi-Nightingale E (October 1969). "Spontaneously occurring bacterial transformations in mice". Journal of Bacteriology. ... the first widely accepted demonstrations of bacterial transformation, whereby a bacterium distinctly changes its form and ... "Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types - Induction of Transformation by ...

*Gene flow

Johnston C, Martin B, Fichant G, Polard P, Claverys JP (March 2014). "Bacterial transformation: distribution, shared mechanisms ... either through transformation (direct uptake of genetic material by a cell from its surroundings), conjugation (transfer of ... genetic material between two bacterial cells in direct contact), transduction (injection of foreign DNA by a bacteriophage ...

*Gram-negative bacteria

Johnston C, Martin B, Fichant G, Polard P, Claverys JP (2014). "Bacterial transformation: distribution, shared mechanisms and ... in the bacterial phylum Firmicutes". Int. J. Syst. Evol. Microbiol. 60 (Pt 6): 1271-9. doi:10.1099/ijs.0.013102-0. PMID ... Transformation is one of three processes for horizontal gene transfer, in which exogenous genetic material passes from ... Transformation has been studied in medically important Gram-negative bacteria species such as Helicobacter pylori, Legionella ...

*Gram-positive bacteria

Johnston, C.; Martin, B.; Fichant, G.; Polard, P; Claverys, J. P. (2014). "Bacterial transformation: distribution, shared ... injection of donor bacterial DNA by a bacteriophage virus into a recipient host bacterium). In transformation, the genetic ... For the bacterial cells bounded by a single cell membrane, the term "monoderm bacteria" or "monoderm prokaryotes" has been ... Transformation is one of three processes for horizontal gene transfer, in which exogenous genetic material passes from a donor ...

*Proteobacteria

Interactive Tree of Life Johnston C, Martin B, Fichant G, Polard P, Claverys JP (2014). "Bacterial transformation: distribution ... Natural genetic transformation is a sexual process involving DNA transfer from one bacterial cell to another through the ... In pathogenic "Proteobacteria", transformation appears to serve as a DNA repair process that protects the pathogen's DNA from ... Many move about using flagella, but some are nonmotile or rely on bacterial gliding. The latter include the Myxobacteriales, an ...

*Unicellular organism

Transformation is a bacterial process for transferring DNA from one cell to another, and is apparently an adaptation for ... Johnston C, Martin B, Fichant G, Polard P, Claverys JP (2014). "Bacterial transformation: distribution, shared mechanisms and ... Kleckner, Nancy; Fisher, Jay K.; Stouf, Mathieu; White, Martin A.; Bates, David; Witz, Guillaume (2014-12-01). "The bacterial ... However, about 80 different species can undergo a sexual process referred to as natural genetic transformation. ...

*Natural competence

In experimental tests, bacterial cells exposed to agents damaging their DNA, and then undergoing transformation, survived ... Most proposals made for the primary evolutionary function of natural competence as a part of natural bacterial transformation ... Redfield R, Schrag M, Dead A (1997). "The evolution of bacterial transformation: sex with poor relations". Genetics. 146 (1): ... Specifically with respect to bacterial transformation, competence requires the high cost of a global protein synthesis switch, ...

*Prokaryote

... transformation is clearly a bacterial adaptation for DNA transfer, because it depends on numerous bacterial gene products that ... also appears to be an accidental process rather than a bacterial adaptation. Play media Natural bacterial transformation ... Chen I; Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-9. doi:10.1038/ ... Harold F (1 June 1972). "Conservation and transformation of energy by bacterial membranes". Bacteriol Rev. 36 (2): 172-230. PMC ...

*Evolutionary history of life

Bacterial transformation is a complex process involving the products of numerous bacterial genes and can be regarded as a ... On the other hand, bacterial transformation is clearly an adaptation for transfer of DNA between bacteria of the same species. ... Sexual reproduction in eukaryotes may have evolved from bacterial transformation. (Also see Evolution of sexual reproduction# ... Bacteria also exchange DNA by bacterial conjugation, the benefits of which include resistance to antibiotics and other toxins, ...

*Ampicillin resistance

... and allowing bacterial cells to transformation. Transformation is the act of taking in extracellular DNA. Most bacteria will ... It is used as a selectable marker in bacterial transformation. A selectable marker is a feature that allows for the scientist ... Ampicillin resistance is bacterial resistance to the antibiotic ampicillin. Many strains of bacteria are resistant to a variety ... However, a few cells will accept it and allow it to integrate into their cell, and now that bacterial cell can produce insulin ...

*Pathophysiology

Lacks SA (Jan 2003). "Rambling and scrambling in bacterial transformation-a historical and personal memoir". J Bacteriol. 185 ( ... However, the transformation of Type I to Type II was the equivalent of the transformation of one species into another, a ... Early work on bacterial transformation, 1928-1940", Profiles in Science, US National Library of Medicine, Web: 24 Jan 2013]. ... A transformation from type to type in vivo presented a disturbing clinical picture, as well as a challenge to the theoretical ...

*Chromosomal crossover

Bacterial transformation itself has been linked to DNA repair many times. The second theory comes from the idea that meiosis ... Thus, this evidence suggests that it is a question of whether cross over is linked to DNA repair or bacterial transformation, ... It is likely that crossing over may have evolved from bacterial transformation, which in turn developed from DNA repair, thus ... The second theory comes from the idea that meiosis evolved from bacterial transformation, with the function of propagating ...

*Avery-MacLeod-McCarty experiment

In 1945, the Royal Society awarded Avery the Copley Medal, in part for his work on bacterial transformation. Between 1944 and ... Hotchkiss, Roland D. "The role of deoxyribonucleotides in bacterial transformations". In W. D. McElroy; B. Glass. Phosphorus ... Fruton (1999), p. 438 The Oswald T. Avery Collection: "Shifting Focus: Early Work on Bacterial Transformation, 1928-1940." ... French microbiologist André Boivin claimed to extend Avery's bacterial transformation findings to Escherichia coli, although ...

*Aliivibrio fischeri

Natural bacterial transformation is an adaptation for transferring DNA from one individual cell to another. Natural ... The bacterial luciferin-luciferase system is encoded by a set of genes labelled the lux operon. In A. fischeri, five such genes ... Natural transformation of A. fischeri facilitates rapid transfer of mutant genes across strains and provides a useful tool for ... The bacterium is a key research organism for examination of microbial bioluminescence, quorum sensing, and bacterial-animal ...

*Rollin Hotchkiss

His work on bacterial transformation helped lay the groundwork for the field of molecular genetics. Hotchkiss was born in South ... In 1951, Hotchkiss showed that purified bacterial DNA could be used to transfer penicillin resistance from one strain of ... Hotchkiss found that virtually all the detected nitrogen in the purified DNA used in for the transformation experiments came ... His subsequent worked helped establish the basics of bacterial genetics, showing that many features of classical genetics ( ...

*Fermented tea

"Lao Man'e: a Bulang Village in Transformation" (PDF). p. 3. Retrieved 12 August 2014. 溫, 志杰; 張, 凌云; 吳, 平; 何, 勇強 (2010), "黑茶加工中微 ... with bacterial fermentation. Batabatacha has been found to contain significant amounts of Vitamin B12. Goishicha (碁石茶) from ...
Bacterial transformation studies have shown that - Biology - Lab 4 (the Bacterial Transformation Lab). Bowtrol Probiotic improve gastrointestinal function & intestinal good bacterial microbial balance.
Pglo bacterial transformation kit - Teachers Manual Pglo Bacterial Transformation Kit Answers. Bowtrol Probiotic improve gastrointestinal function & intestinal good bacterial microbial balance.
Join the Bay Area community of bioscience teachers for a day of hands-on learning and collaboration in bringing to your students a NGSS-ized Bacterial Transformation experiment.. ...
Opa proteins are major proteins involved in meningococcal colonization of the nasopharynx and immune interactions. Opa proteins undergo phase variation (PV) due to the presence of the 5-CTCTT-3 coding repeat (CR) sequence. The dynamics of PV of meningococcal Opa proteins is unknown. Opa PV, including the effect of transformation on PV, was assessed using a panel of Opa-deficient strains of Neisseria meningitidis. Analysis of Opa expression from UK disease-causing isolates was undertaken. Different opa genes demonstrated variable rates of PV, between 6.4 × 10(-4) and 6.9 × 10(-3) per cell per generation. opa genes with a longer CR tract had a higher rate of PV (r(2) = 0.77, p = 0.1212). Bacterial transformation resulted in a 180-fold increase in PV rate. The majority of opa genes in UK disease isolates (315/463, 68.0%) were in the on phase, suggesting the importance of Opa proteins during invasive disease. These data provide valuable information for the first time regarding meningococcal Opa PV.
Many bacteria are highly sexual, but the reasons for their promiscuity remain obscure. Did bacterial sex evolve to maximize diversity and facilitate adaptation in a changing world, or does it instead help to retain the bacterial functions that work right now? In other words, is bacterial sex innovative or conservative? Our aim in this review is to integrate experimental, bioinformatic and theoretical studies to critically evaluate these alternatives, with a main focus on natural genetic transformation, the bacterial equivalent of eukaryotic sexual reproduction. First, we provide a general overview of several hypotheses that have been put forward to explain the evolution of transformation. Next, we synthesize a large body of evidence highlighting the numerous passive and active barriers to transformation that have evolved to protect bacteria from foreign DNA, thereby increasing the likelihood that transformation takes place among clonemates. Our critical review of the existing literature provides ...
Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; chemical transformation and electroporation. There are advantages and disadvantages to both transformation methods. In general, chemical transformation is less prone to error and faster however electroporation produces a higher transformation efficiency (fraction of transformed cells that actually uptake the foreign DNA). See Molecular Cloning for a fuller discussion of both approaches. ...
Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called "competent". Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; chemical transformation and electroporation. There are advantages and disadvantages to both transformation methods. In general, chemical transformation is less prone to error and faster however electroporation produces a higher transformation efficiency (fraction of transformed cells that actually uptake the foreign DNA). See Molecular Cloning for a fuller discussion of both approaches. ...
BIO-A #6A: In this experiment, you will Use a plasmid vector to transform bacteria with genes for Green Fluorescent Protein (GFP) and antibiotic resistance in a controlled experiment. Use the heat shock method of transforming E. coli. Regulate the expression of the GFP gene using arabinose. Describe the biological process involved in transforming bacterial cells. Calculate your transformation efficiency. Learn basic molecular biology techniques.
Demonstrate the power of genetic transformation! Students will glow with excitement when they transform bacteria with pGLO plasmid. Ideal for AP Biology Lab 6.
The first step in the lab is to acquire two micro tubes, one for the - plasmid and one for the + plasmid. The next step is to use a pipette to add 250 micro liters of CaCl2 or transformation fluid into each tube. Then take a sterile inoculation loop and acquire a colony of E.coli bacteria from the starter plate. Add the bacteria to one of the micro tubes by submersing it in the transformation fluid and by spinning the loop. Then repeat this step but add the bacteria to the other tube. Next pipette 10 micro liters of plasmid solution into the + plasmid micro tube. Then make sure both of the tubes are sealed tight and incubate them in ice for 10 minutes (make sure the tops of the tubes are not submerged in the ice). While you are waiting you can label your agar plates (LB/AMP - pGLO, LB, LB/amp +pGLO, LB/amp/ara). When the tubes are done in the ice put them in a test tube float and insert it into a water bath set at 42 degrees Celsius for only 50 seconds. Next put te tubes back on ice for two ...
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Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Tips and interesting applications using PASCO sensors, software and equipment.. Have innovative lab ideas youd like to share? Wed love to hear from you!. ...
Author Summary Genetic elements, such as transposons and integrons, frequently carry antimicrobial resistance determinants and can be found widely disseminated among pathogenic bacteria. Their distribution pattern suggests dissemination through horizontal gene transfer. The role of natural transformation in horizontal transfer of genetic elements other than those that are self-replicative (plasmids) has remained largely unexplored. We have tested if natural transformation can facilitate transfer of transposons and class 1 integrons between bacterial species. We here provide experimental evidence showing that natural transformation can be a general mechanism for dissemination of genetic elements that by themselves do not encode interspecies transfer functions (e.g. transposons, insertion sequences). We demonstrate that antibiotic resistance determinants present in such genetic elements can spread by natural transformation between species of clinical interest. We show by quantitative data that
TY - JOUR. T1 - Putative mechanism of natural transformation as deduced from genome data. AU - Yura, Kei. AU - Toh, Hiroyuki. AU - Go, Mitiko. PY - 1999. Y1 - 1999. N2 - Genetic transformation is widely utilized in molecular biology as a tool for gene cloning in Escherichia coli and for gene mapping in Bacillus subtilis. Several strains of eubacteria can naturally take up exogenous DNA and integrate the DNA into their own genomes. Molecular details of natural transformation, however, remained to be elucidated. The complete genome of a cyanobacterium, Synechocystis sp. PCC6803, has been sequenced. This bacterium has been used to examine functions of a particular gene. The genome is considered to carry information on natural transformable characteristics of Synechocystis. The first step in genetic transformation is the uptake of exogenous DNA. Proteins with non-specific DNA binding features are required, because specificity in the exogenous DNA has not been demonstrated. Such proteins have modules ...
pGLO™ & GFP Central Framework of Molecular Biology DNA RNA Protein Trait What is Transformation? • Uptake of foreign DNA, often a circular plasmid GFP Beta-lactamase Ampicillin Resistance What is a plasmid? • A circular piece of autonomously replicating DNA • Originally evolved by bacteria • May express antibiotic resistance gene or be modified to express proteins of interest Protein Size • Beta Lactamase - Ampicillin resistance • Green Fluorescent Protein (GFP) - Aequorea victoria jellyfish gene • araC regulator protein - Regulates GFP transcription Transformation Procedure Day 1 Day 2 Bacterial Transformation Cell wall GFP Bacterial chromosomal DNA Beta lactamase (ampicillin resistance) pGLO plasmids Bacterial DNA Bacterial cell Plasmid DNA Genomic DNA Transcriptional Regulation • Lactose operon • Arabinose operon • pGLO plasmid Methods of Transformation • Electroporation - Electrical shock makes cell membranes permeable to DNA • Calcium Chloride/Heat-Shock - ...
Edvotek. In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have …
The next steps in DNA cloning are bacterial transformation and selection. Once we have the recombinant DNA with your gene of interest, it is inserted into bacteria (transformation) and those bacteria that contain the plasmid were looking for are selected. We can then induce those bacteria to produce the protein were interested in (whether it is red fluorescent protein or a medical product such as insulin) and purify it ...
Theres a lot of degraded DNA in the environment. Its estimated that more than 1,000 tons of sedimentary DNA is released by rivers every year. The detritus is from plants and animals that have reached the end of life by natural and not so natural means. With soil constantly being exposed through erosion, glacial retreat, and overflowing rivers, some of this DNA could be several centuries old. With that amount of degraded DNA lying around, is it possible that bacteria could absorb some of it into their genomes? Led by Søren Overballe-Petersen, Ph.D., researchers at the University of Copenhagen decided to find out. Most of the free DNA in the environment is , 100 bp. The team set out to find the lower limit of bacterial transformation, identifying a process that isnt dependent on the presence of RecA, the protein that integrates DNA into the genome. This basal transformation occurred with fragments down to 20 bp. It even occurred with short modern DNA that had been damaged with uracils, ...
The DNA Blunting Kit allows the conversion of 3 and 5 overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3 to 5 exonuclease and 5 to 3 polymerase activities of T4 DNA Polymerase. The resulting blunt-ended DNA can be ligated efficiently into a vector using the same optimized buffer system employed in Takaras DNA Ligation Kits. The reaction can then be used directly in bacterial transformation or in vitro packaging procedures without need for further DNA purification.. ...
This laboratory course introduces students to methods for manipulating and analyzing nucleic acids. Students gain extensive hands-on experience with plasmid purification, restriction mapping, ligations, bacterial transformations, gel electrophoresis, as well as applications of the polymerase chain reaction. This course is not recommended for students with substantial experience in these methodologies. Prerequisites: 410.601 Biochemistry; 410.602 Molecular Biology.. ...
trouble with plasmid transformation in BL21DE3 E. coli cells - posted in Molecular Cloning: Hi everyone, I have a gene cloned in pRSETa vector that has an ampicillin resistance selection. I transformed this plasmid in BL21DE3 E.coli expression system and I get no colonies. I tried checking the amp concentration on LB plates, changed the comp cells but the problem never resolved. I simultaneously transformed this clone in rosetta cells and bingo! I get the colonies. Now, the problem is,...
Aqueorea victoria---the source of the GFP gene in pGLO. ANNOUNCEMENTS. Check out these awesome resourses: Science Daily (news site). DNA Learning Center. IFL science :). LECTURE MATERIALS. Syllabus 2016. General expectations. Powerpoint lectures:. Lecture 1. Lecture 2. Lecture 3. Lecture 4. Lecture 5. Lecture 6. Lecture 7. Lecture 8. Lecture 9. Lecture 10. -------------. Lecture 11. Animation Essential Biochemistry - DNA Replication. DNA replication 2. Lecture 12. Lecture 13. Lecture 14. Lecture 15. Lecture 16. Lecture 17. Lecture 18. Lecture 19. Lecture 20. Lecture 21. Lecture 22. Lecture 23. Lecture 24. Lecture 25. Lecture 26. Lecture 27. Lecture 28. Lecture 29. Lecture 30. Lecture 31. Lecture 32 Beta galactosidase paper. Lecture 33. Lecture 34. Lecture 35. Lecure 36---I will bring copies to class.. Topics Lists for Exams. Topics list 1. Topics list 2. Topics list 3. Topics list 4. Histones in replication. LAB MATERIALS. Lab syllabus: here. Lab 1Bacterial Transformation/DNA IS the genetic ...
Intra-peritoneal injection and oral gavage on mice; organs and embryos harvesting. - Preparation of primary cultures (cardiac explants) and growth of cardiac progenitor cells and mESCs. - Langendorff preparation to disperse cells from the heart. - Cloning and transfection of cells in culture. - Cellular characterization by Immunohistochemistry, Immunofluorescence and FACS sorting. - Proteins extraction and Western Blotting. - RNA and DNA extraction and purification. - PCR (Polymerase Chain Reaction)/ RT-PCR (Sybr Green and Taqman). - Bacterial cultures and bacterial transformation Good knowledge of Bioinformatic tools. Use of ImageJ, OligoExplorer, BLAST. Basic knowledge of CorelDraw, CLUSTALW, Rasmol v.2.6, Swiss Pdb Viewer, Clone, Gene Runner. ...
Free flashcards to help memorize facts about Vocablary for heredity, bacterial transformation and Reebops. Other activities to help include hangman, crossword, word scramble, games, matching, quizes, and tests.
RESEARCH INTERESTS Dr. Pascal Simonet obtained his PhD in 1983. Currently his research group investigates the evolution potential of bacteria in environments such as the soil and plants. For more than 15 years the research group entitled « Gene Transfer and Bacterial Adaptation » that he led at the University of Lyon has had the general objectives of determining the involvement of horizontal gene transfers (HGT) in the adaptation and evolution of bacteria to new environments. Studies of the group focused mainly on natural genetic transformation and also, but at a lesser extent, on conjugation. These objectives lead the group to develop soil DNA extraction methods and it was among the first to investigate environmental bacteria with metagenomic approaches. Several of the studies were devoted to investigate the fate of DNA released by genetically engineered organisms including the possibility that recombinant DNA, and particularly antibiotic resistance genes transforms indigenous bacteria. The ...
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
3. Each mutant strain does have more Sxy protein (between 3-fold and 50-fold more, depending on the mutation and the growth conditions) (Fig. 3A). The competence of each strain (measured as transformation frequency) increases with the amount of Sxy protein in the cells (Fig. 3B). How could the mutations cause this? We propose that they cause this by interfering with how part of the sxy mRNA folds back on itself. The folding lets the two different parts of the mRNA where the mutations occur form base pairs with each other (Fig. 4 ...
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Author Summary Many bacteria can actively acquire novel genetic material from their environment, which leads to the rapid spreading of, for example, antibiotic resistance genes. The bacterium Bacillus subtilis can differentiate into the state of competence, in which cells take up ssDNA through a DNA uptake complex that is specifically localized at a single cell pole. DNA can be integrated into the chromosome, via RecA, or can be reconstituted as circular dsDNA, if derived from plasmid or from viral DNA. We show that RecO, RecU, and Ku proteins, but not RecA, are important for plasmid transformation, and differentially accumulate at the polar DNA uptake machinery. Upon addition of any kind of DNA, the assembly of RecU at the competence pole dissipated, while RecA formed filamentous structures that rapidly grew and shrank within a 1 minute time scale. RecO visibly accumulated at the competence machinery only upon addition of plasmid DNA, but not of chromosomal DNA. In vitro, RecO was highly efficient at
Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. This is based on the competence of the cells. It can be calculated by dividing the number of successful transformants by the amount of DNA used during a transformation procedure. Transformants are cells that have taken up DNA (foreign, artificial or modified) and which can express genes on the introduced DNA. Transformation efficiency should be determined under conditions of cell excess. The number of viable cells in a preparation for a transformation reaction may range from 2×108 to 1011; most common methods of E. coli preparation yield around 1010 viable cells per reaction. The standard plasmids used for determination of transformation efficiency in Escherichia coli are pBR322 or other similarly-sized or smaller vectors, such as the pUC series of vectors. Different vectors however may be used to determine their transformation efficiency. 10-100 pg of DNA may be used for ...
I expect the E. coli-grown plasmid to transform quite poorly, because the DNA will lack methylation at the HindII and HincII restriction sites. In my first experiment the difference was dramatic: I got 10,000 transformants into E. coli but none into H. influenzae. The Rd strain of H. influenzae carries both of these restriction systems (Ham Smith got a Nobel Prize for discovery of these), and its cytoplasm is chock full of the HindIII and HincII restriction enzymes. Although its own DNA is appropriately methylated and thus immune to cleavage, incoming DNA from other species is cut. The restriction enzymes only cut double-stranded DNA so they dont affect chromosomal transformation (watching the DNA uptake movie might help this make sense), but plasmids introduced by electroporation or plasmid transformation remain double-stranded, so theyre vulnerable ...
DNA Sequence Analysis. Broad and Long Term Objective. To characterize a single clone from an Emiliania huxleyi cDNA library using sequence analysis. Research Plan. Preparation of Competent Cells and Bacterial Transformation. Growth of Transformant and Plasmid MiniPrep. Cycle Sequencing. Slideshow 6718522 by gabriel-russell
Molecular Biology: Students are introduced to the basics of molecular biology, with an emphasis on the exciting field of Biotechnology. Topics of study include the human genome and genomics, recombinant DNA technology and cloning, gene expression, and an introduction to gene editing techniques, such as RNA interference and CRISPR/Cas9. Students laboratory experiences include: isolation of genomic DNA, bacterial transformation and gene expression, DNA fingerprinting and forensics. They discover how these techniques are applied to such diverse purposes as criminal investigations, testing foods for genetic modification, disease diagnosis and the development of new therapies and pharmaceutical products.. Cell Biology: Students are introduced to the basic facts of cell biology, including cell morphology and physiology, and learn about the macroscopic and microscopic aspects of reversible and irreversible cell injury. Students also study the morphology of cancer cells in comparison to normal healthy ...
O experimento de Griffith, relatado em 1928 por Frederick Griffith, foi o primeiro experimento sugerindo que bactérias são capazes de transferir informação genética através de um processo conhecido como Transformação. A descoberta de Griffith foi seguida pela do final da década de 1930 e início da década de 1940 que isolou o DNA como o material que comunicava esta informação genética. Griffith, Fred. (janeiro de 1928). «The Significance of Pneumococcal Types» 2 ed. Cambridge University Press. Journal of Hygiene. 27: 113-159. JSTOR 4626734. PMC 2167760. PMID 20474956. doi:10.1017/S0022172400031879 Lorenz, M. G.; Wackernagel, W. (1 de setembro de 1994). «Bacterial gene transfer by natural genetic transformation in the environment» 3 ed. Microbiological Reviews. 58: 563-602. PMC 372978. PMID 7968924 Downie, A. W. (1972). «Pneumococcal transformation - a backward view: Fourth Griffith Memorial Lecture» (PDF) 1 ed. Journal of General Microbiology. 73: 1-11. PMID 4143929. ...
In order to achieve the functionality of pneumosensor, we must have a highly specific reporting system which will only give fluorescent signal under the presence of S. pneumoniae. In search for the suitable gene circuit, the discovery by Prof. Morrison on the competence for genetic transformation in S. pneumoniae which depends on quorum-sensing system to control many competence-specific genes acting in DNA uptake, processing, and integration has provided the ideal framework for this module. (Lee and Morrison, 1999) There is a link between this quorum-sensing system and the competence-specific genes, which is an alternative σx (ComX protein) that serves as a competence-specific global transcription modulator. (Luo and Morrison, 2003) In S. pneumoniae, competence (a state capable of being genetic transformed) happens transiently during the log phase growth, and is regulated by a quorum sensing system utilizing the Competence Signal Peptide (CSP). Upon stimulation by CSP, σx will be expressed and ...
Genetic transformation is the method by which a receiver bacterial cell usually takes up DNA from a neighboring mobile and integrates this DNA to the receivers genome by recombination. In N. meningitidis, DNA transformation involves the existence of short DNA sequences (9-10 mers residing in coding areas) on the donor DNA. These sequences are termed DNA uptake sequences (DUSs). Specific recognition of these sequences is mediated by a type IV pilin ...
The soil organism Bacillus subtilis has the ability to take up DNA from its environment and, provided there is homology, recombine it into its genome. This process is called transformation. In order for the bacterium to be transformed it must be in a specific physiological state called competence. During the competent state specific proteins are synthesized which are required for the binding and transport of the DNA molecule into the competent cell. It has been found that as transformability increases many competence specific proteins localize to the poles of the rod shaped Bacillus subtilis bacterium and as transformability declines the competence proteins delocalize. These proteins colocalize and interact and seem to form a complex that helps internalize the DNA molecule, preferentially at the poles of the cell. Jeanette Hahn s focus is understanding how the polar localization and delocalization of the competence proteins occurs. Localization seems to occur via a diffusion and capture ...
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Oral Presentations Cellular Regulation: From Cells to Human Health Natural Transformation: The role of ecsA and ecsB in Bacillus subtilis Christian Loyo, University of Wisconsin-Madison Natural transformation is the process of translocating exogenous DNA from the environment into a cell and incorporating it into the genome. As a mode of horizontal gene…
I always prepare fresh competent cells by Hanahan Method using TFB buffer and DnD (Sambrook). I would like to prepare frozen stocks of competent cells by this method, using FSB buffer and DMSO as the protocol describes. At the end the protocol, It says you have to snap-freeze the competent cells by immersing the tubes in liquid nitrogen and then store at -70°C until needed ...
A paper just came out in PNAS entitled "Promotion of direct reprogramming by transformation-deficient Myc". The main thrust of this paper is that the tumorigenic and pluripotency-related functions of Myc could be separated. It focused primarily on the lesser studied […]. ...
For transformation of PCR-TRAP and pAPtag-5 vectors. The GH Competent cells can be used with both our PCR-TRAP PCR product cloning system & with our pAPtag-5 AP fusion cloning vector (AP-TAG Kit B). The pAPtag-5 vector contains the ampicillin resistance gene. It can be easily and efficiently transformed and propagated in GH Competent cells.. 3 mLs (6 x 0.5 mL tubes) Detailed protocol included.. ...
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Edvotek Series 200 Experiments. For 10 Transformations and controls. Time Required: Transformation -- 45 min.; Plating -- 5 min.; Incubation -- overnight; Transformation Efficiency …
Are there any reasons why transformation of bacteria with linear DNA should not work? Does it work? any comments appreaciated. Thanks for your help ...
The nucleus is a very crowded place, filled with DNA, proteins packing up DNA, proteins patching up DNA, proteins opening up DNA to transcribe it etc. Statements like this produce no physical intuition of the sizes of the various players (to me at least). How do you go from the 1 Angstrom hydrogen atom, the…
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description?: late competence operon required for DNA binding and uptake comEB. descriptions from strain specific annotations: ...
Express difficult or even toxic proteins using any E. coli T7 vector. Available as chemically competent or electrocompetent cells.
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Developed in 2011. Atlantic Sea Scallop Dredge Design Challenge (Grades 9 & 10). The Cell Cycle and Mitosis (Grades 8 & 9). Conducting Action Research on the Impact of Environmental Issues on Community Health (Grades 9 & 10). Genetic Mutations (Grades (9 & 10). Kidney Dissection (Grades 9 & 10). Lifesaver Protein Modeling (Grades 9 - 12). Developed in 2010. Genetic Variation (Grades 9 & 10). Investigating Factors That Affect Rate of Enzyme Action (Grades 9 & 10). Micropipette and the Metric System (Grades 9 & 10). Interactive Meiosis (Grades 9 & 10). Modeling Protein Structure. Thinking Like a Scientist (Grades 9 & 10). Understanding Kidney Disease (Grades 3-5). Whose Brain is THAT?!. Developed in 2009. Bacterial Transformation. Blood Analysis. Blood, I Presume?. Concept Mapping. DNA Extraction From Living Things. Investigating Factors That Affect Cell Membrane Permeability. Lets Grow Some Microbes!. Sheep Brain Dissection. Developed in 2008. Amino acids and protein. Amoeba this!!!! Amoeba ...
Detection of Horizontal Gene Transfer by Natural Transformation in Native and Introduced Species of Bacteria in Marine and Synthetic Sediments (Printed Ephemera) : Stewart, Gregory John
Artificial competence of Synechococcus PCC 6301 cells was induced by lysozyme treatment and the cells were transformed to chloramphenicol resistance with foreign plasmid pBR325 at a frequency of approximately 5 times 10-5 or 5 times 10-4 with the transformant DNA. The transformation frequencies were higher than those reported by other workers for the same strain with cloned DNA employing a physiological transformation system. Analyses of DNA electrophoresis, secondary transformation and dot blotting demonstrated that foreign plasmid had integrated into the recipient chromosome by a single crossover event. The results showed that the artificial transformation system was efficient and reproducible. Conditions that affected transformation, such as, incubation time of cells with DNA, age of the cells, light or dark incubation were also studied ...
An image processing method is provided for reducing noise in a sampled image, particularly for reducing noise in an image divided into blocks of sampled image elements that are transformed by a linear procedure, such as the Walsh-Hadamard transform, and improved regarding visible noise by non-linear thresholding of the transform coefficients. By operating the process in a hierarchy of stages, each stage employing a block operating on image signals derived from a preceding stage, and by overlapping the blocks processed in each stage, the processed signal from each image element is the linear combination of many transform coefficients from each stage and from each overlapped block within each stage. Such a large number of contributions making up each processed image element assures that the processed image is generated without a characteristic block-like structure due to block transform processing while the wanted components of the image are rendered with minimal image loss or distortion.
ELISA Kit,antibodies,plasmid,Competent Cells,strains : Ask a Question - ELISA KIT Primary Antibody Protein Peptide Competent Cells CLIA kit Plasmid Strains Plasmid Extraction Lysis Buffer Reagent Antibody ELISA Kit,antibodies,plasmid,Competent Cells,strains
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NEB offers several stains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies. NEB Express is an enhanced BL21 derivative available with (NEB #C3037) or without (NEB #C2523) the added control from lacIq.. Within the realm of E. coli expression, the T7 system is the most popular approach for producing proteins. In this system, an expression vector containing a gene of interest cloned downstream of the T7 promoter is introduced into a T7 expression host. T7 expression hosts, such as DE3 strains or T7 Express strains, carry a chromosomal copy of the phage T7 RNA Polymerase gene, which is controlled by a lac promoter. When inducer is added, T7 RNA Polymerase is expressed and becomes dedicated to transcription of the gene of interest. T7 Express strains are available with the control features lacIq and/or lysY. The lysY gene expresses a variant T7 lysosome which naturally inhibits T7 RNA Polymerase, thereby controlling basal ...
The aim of this work was to develop a method to evaluate the kinetics of bainite transformation by theoretical deduction and thermal dilatation curve analysis. A Gleeble-3500 thermomechanical simulator and dilatometer (DIL805A) were employed to study the isothermal transformation in deformed (360 ∘ C , 600 ∘ C , and 860 ∘ C ) and undeformed conditions. The thermal dilatation information during isothermal transformation was recorded, and the dilatation curves were well smoothed. By taking a derivative of the dilation curve with respect to the transformation time, the peak time of transformation rate (PTTR) was obtained, which can serve as the essence of isothermal transformation time. The relative change of length ( Δ L / L ) due to phase transformation was theoretically deduced, and the effect of temperature was taken into consideration. Combing experimental data, the volume fraction of bainite in isothermal transformation was calculated. Making a graph of
Dear All, I just want to know that how long could the competent cells be stored at -70degC? I had prepared the competent cells (for electroshock tranformation) just one month back. Thanks for your input in advance. with best regards, Sulakshana ...
Ith DpnI (1 hour at 37uC) and transformed into E-coli competent cells (Stratagene). All mutated plasmids were checked by sequencing and primer sequences are
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Nucleotides are the sub-units that make up DNA, and are made up of even smaller sub-units. The differences between these sub-units result in the unique base pairs that record genetic information and make up genes. More » ...
Whenever an Xtend function is invoked - whether for M2M Xtend transformations or M2T Xpand templates, a Java Model Exception is thrown ...
If Y is uniformly distributed over the interval (0,1), i.e. f(y) = 1 for 0| y | 1. Show that U = -2 loge(Y) has a negative exponential
Streptococcus infantarius is a species of bacteria. S. infantarius is competent for natural genetic transformation. Competence can be induced in cultures at high cell density, and is transient. Schlegel, L.; Grimont, F.; Collins, M. D.; Regnault, B.; Grimont, P.; Bouvet, A. (2000). "Streptococcus infantarius sp. nov., Streptococcus infantarius subsp. infantarius subsp. nov. and Streptococcus infantarius subsp. coli subsp. nov., isolated from humans and food". International Journal of Systematic and Evolutionary Microbiology. 50 (4): 1425-1434. doi:10.1099/00207713-50-4-1425. ISSN 1466-5026. PMID 10939646. Morrison DA, Guédon E, Renault P (2013). "Competence for natural genetic transformation in the Streptococcus bovis group streptococci S. infantarius and S. macedonicus". J. Bacteriol. 195 (11): 2612-20. doi:10.1128/JB.00230-13. PMC 3676052 . PMID 23543718. Corredoira, J.; Alonso, M. P.; Coira, A.; Varela, J. (2008). "Association between Streptococcus infantarius (Formerly S. bovis II/1) ...
Plasmid transformation in Leuconostoc carnosum 4010 was analyzed. A successful transformation protocol for L. carnosum was established by modifying an existing protocol for Lactococcus lactis. Several parameters, including the number of generations that the cells had grown at the time of harvest, glycine concentration, the time of incubation for phenotypic expression, and the electrical field strength, were investigated and proved to have influence on the transformation frequency. Electrocompetence was found to be transient and to peak in the early exponential growth phase. Optimized conditions resulted in transformation frequencies of up to 6.7 x 10(5) transformants per microgram of plasmid DNA. A total of five plasmids in L. carnosum were successfully introduced and maintained. Interestingly, we discovered that DNA uptake was of a frequency of 3 x 10(-6) to 19 x 10(-6) transformants per CFU in the absence of an applied electrical field. We concluded that L. carnosum is naturally competent ...
The physiological state of natural competence for transformation allows certain bacteria to take up free DNA from the environment and to recombine such newly acquired DNA into their chromosomes. However, even though conserved components that are required to undergo natural transformation have been identified in several naturally competent bacteria, our knowledge of the underlying mechanisms of the DNA uptake process remains very limited. To better understand these mechanisms, we investigated the competence-mediated DNA transport in the naturally transformable pathogen Vibrio cholerae. Previously, we used a cell biology-based approach to experimentally address an existing hypothesis, which suggested the competence protein ComEA plays a role in the DNA uptake process across the outer membrane of Gram-negative bacteria. Here, we extended this knowledge by investigating the dynamics of DNA translocation across both membranes. More precisely, we indirectly visualized the transfer of the external DNA ...
In streptococci, entry in competence is dictated by ComX abundance. In Streptococcus thermophilus, production of ComX is transient and tightly regulated during growth: it is positively regulated by the cell-cell communication system ComRS during the activation phase and negatively regulated during the shut-off phase by unidentified late competence gene(s). Interestingly, most S. thermophilus strains are not or weakly transformable in permissive growth conditions (i.e. chemically defined medium, CDM), suggesting that some players of the ComRS regulatory pathway are limiting. Here, we combined mathematical modeling and experimental approaches to identify the components of the ComRS system which are critical for both dynamics and amplitude of ComX production in S. thermophilus. We built a deterministic, population-scaled model of the time-course regulation of specific ComX production in CDM growth conditions. Strains LMD-9 and LMG18311 were respectively selected as representative of highly and weakly
Also, DNA damaging conditions induces competence in a cell. For example, exposure to UV light, a DNA damaging agent. By electrifying the plasma membrane of a cell, you can alter its permeability by creating hydrophilic pores. How does this work? DNA is a polar molecule and, like water, does not easily pass through the hydrophobic regions of the membrane. However, once pores are made within the membrane, the genetic material can easily pass through. The more coiled the DNA is, the better its chances of being taken up during transformation are. This is why plasmids are used instead of linear DNA fragments. Also the DNA usually has an antibiotic-resistance marker, to help identify the successfully transformed bacteria. (chemical competence) It can still work! Transformation is still used to alter plant cells. Many plants can regrow from a single cell, making them optimal for genetic modification via transformation. Transformation can also occur naturally in parts of plants, though it doesnt ...
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With current advances in sequencing technologies (link to sequencing section), however, blight-resistant genes from Chinese and Japanese chestnuts may soon be identified, cloned, and used in this process.. After you have your gene complex is created, the next step is to figure out a way to get it into your species of interest. There are a few ways to do this, but the easiest and current method used for chestnut is Agrobacterium-mediated transformation. After the transformation is completed, the next stage is to get the tree out into the field. There are several steps involved in this, and the resulting treelets need to be handled very carefully until they are ready to be put outside. Transformed embryonic tissue need to first be multiplied and start turning into shoots. The resulting shoots are then rooted in tissue culture boxes, where only 10-60% of them may actually develop roots. After the rooting cycle, the treelets need to be acclimatized and readied for the outdoors ...
4 credits. Last Update Effective: 8/21/16. Biotechnology field, laboratory techniques, applications, and bioethical considerations. Metric system, solutions, spectrophotometry, bacteria culturing, gel electrophoresis, plasmid transformation and purification, polymerase chain reaction, Deoxyribonucleic Acid (DNA) structure, recombinant techniques, quantitation, sequencing, and microarray.. Prerequisite: BIS 222◊, BOT 200, CHM 110◊ or CHM 140◊, MAT 110◊ or MAT 111◊. Lecture: 2 ...
Possible mechanism for donor DNA binding and transport in Haemophilus.: Morphological differences were observed in competent and noncompetent Haemophilus parain
BL21 Chemically Competent Cells are provided in aliquots of 80 μL sufficient for two transformation reactions of 40 μL each. Transformation is performed by heat shock at 42°C, followed by incubation on ice.
Diversities have recently been developed as multiway metrics admitting clear and useful notions of hyperconvexity and tight span. In this note, we consider the analytical properties of diversities, in particular the generalizations of uniform continuity, uniform convergence, Cauchy sequences, and completeness to diversities. We develop conformities, a diversity analogue of uniform spaces, which abstract these concepts in the metric case. We show that much of the theory of uniform spaces admits a natural analogue in this new structure; for example, conformities can be defined either axiomatically or in terms of uniformly continuous pseudodiversities. Just as diversities can be restricted to metrics, conformities can be restricted to uniformities. We find that these two notions of restriction, which are functors in the appropriate categories, are related by a natural transformation.
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Access the latest market research report on Global Competent Cells Market 2018 by Manufacturers, Countries, Type and Application, Forecast to 2023 with complete table of contents and segmentations.
I had cloning issues that sounds very similar to yours (somewhere within the gel extraction step). I finally found the issue was related to the quality of the agarose. I tried 4 different brands of agarose (normal agarose and low melting point agarose, old and new), each yielded DNA. However, transformation with 3 different brands of agarose did not yield any colonies (gel extracted uncut vector). Contaminants in old agarose or how the agarose was made by the company appears to be a major factor in transformation efficiency ...
Apart from BactoDHL, a synthetic biology creation, there are many different protein delivery systems derived from naturally-invasive, attenuated pathogens like Salmonella ssp. and Listeria ssp., which seem to show great potential. There are three main reasons why the makers of BactoDHL decided to create a new invasive microorganism instead of following the alternative path mentioned above:. First and foremost the laboratory E. coli strain chosen cannot successfully thrive in the cytoplasm of a mammalian cell.. Secondly the this strain of E. coli has no natural competence, so there is no risk of it picking up pathogenic genes from the environment.. Thirdly the bacterium will be equipped with a kill switch in the form of a MinC, a protein that acts upon the cytoskeleton and prevents proliferation.. ...
Transformations utilizing a 168-like recipient and mixtures of homologous and heterologous DNA lead to an unexpected increase in the number of transformants when the two DNAs are in equal concentration. The absolute requirement for native heterologous DNA to produce the effect was demonstrated. The increase may be due to a helping effect analogous to that found in Streptococcus.
Drinking alcohol has been found to damage Deoxy ribonucleic Acid (DNA)/genetic material - causing an increased chance of breast cancer and other major types of the disease.
GH Competent Cells. $230.00 For transformation of PCR-TRAP and pAPtag-5 vectors The GH Competent cells can be used with both our PCR-TRAP PCR product cloning system & with our pAPtag-5 AP fusion cloning vector (AP-TAG Kit B). The pAPtag-5... More Info ...
View Notes - BMI_Lab8DNAPurification from PHS 2301 at St. Johns Duplicate. Laboratory VIII Topic: Purification of DNA, plasmid isolation using alkaline method Introduction: Genetic transformation
Lucigen strives to provide life scientists with the highest quality products and services for RNA/DNA amplification, cloning, next gen sequencing, and protein expression. Experience outstanding performance with time-saving convenience at an exceptional price.
The following guide can be used for troubleshooting transformation reactions. You may also be interested in reviewing additional tips for Chemical Transformation or Electroporation
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We have found 3 NRICH Mathematical resources connected to Enlargements and scale factors, you may find related items under Transformations and constructions
Nuclear transformation occurs when the atoms of one element change to become atoms of another element. This is most commonly seen with...
PRO-TF Protein, Protein Bars, and Renuvo will change your life and give you that Transformation you have always been searching for.
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The excited husband shared the amazing transformation with his fans online. He wrote: Here is the little project my wife and I have been working on the last 365 days, she has lost 58lbs and I have lost 224lbs
Its officially the New Year, a time to make a fresh start and renewed plans. And were wondering…are you finally ready to commit to a plan to help you achieve healthier-looking skin? For those new to Nu-Derm If you dont know about or arent using the Obagi Nu-Derm® System* yet, allow us to introduce you to our flagship transformation system and the #1 physician-dispensed skin care system.
Impediments to Change: In considering the forces for change, the business decision to be made, and their organizational consequences, leaders need to choose between treating the change in an incremental and linear way or in a.
Horizontal gene transfer is an important source of genetic variation among Neisseria species and has contributed to the spread of resistance to penicillin and sulfonamide drugs in the pathogen Neisseria meningitidis. Sulfonamide resistance in Neisseria meningitidis is mediated by altered chromosomal folP genes. At least some folP alleles conferring resistance have been horizontally acquired from other species, presumably from commensal Neisseriae. In this work, the DNA sequence surrounding folP in commensal Neisseria species was determined and compared to corresponding regions in pathogenic Neisseriae, in order to elucidate the potential for inter-species DNA transfer within this region ...
The Neisseria species are believed to be non-clonal bacteria with a high degree of genetic transfer within and between different species [2, 3, 14-18]. Previous reports of transformation in N. meningitidis have suggested that different regions of the genome may have different recombination rates [19, 20]. This work identified the region upstream of folP as particularly active in recombination events. The most obvious example is the insertion of a complete Correia element, upstream of folP in N. lactamica. Correia elements are small putative transposable elements, around 152 base pairs long with inverted repeats at the ends [12]. Hundreds of Correia copies are scattered around the genomes of pathogenic Neisseriae [13, 21, 22] and they have also been reported in N. lactamica [23]. We detected another copy of a Correia repeat in the N. subflava sequence, suggesting that these elements are ubiquitous in the Neisseria genus and possibly was introduced before the divergence into different ...
Haemophilus influenzae is a gram-negative bacterium that exclusively colonizes humans. Nonencapsulated strains of the bacterium are found in the upper respiratory tract of healthy humans, but can also cause the respiratory diseases otitis media, bronchitis, and pneumonia. Many of the chromosomal genes of H. influenzae were acquired by lateral transfer from other bacterial genera. Recently, we investigated a cluster of unusually virulent nonencapsulated H. influenzae implicated in human invasive disease. An island between aspA and groES in which a urease gene cluster present in H. influenzae had been replaced by a homolog of Neisseria meningitides mtrF was discovered (Erwin et al., 2005). We compared the aspA-groES region with that of Pasteurella multocida, a member of the same family of bacteria. The two genomes have scattered synteny and share about 83% of their DNA. Both species have natural genetic transformation and use the same recognition site for DNA uptake. The mtrF gene is found between ...
25 APPENDIX C. FINDING MEDICAL LIBRARIES Overview In this Appendix, we show you how to quickly find a medical library in your area. Preparation Your local public library and medical libraries have interlibrary loan programs with the National Library of Medicine (NLM), one of the largest medical collections in the world. According to the NLM, most of the literature in the general and historical collections of the National Library of Medicine is available on interlibrary loan to any library. 15 Finding a Local Medical Library The quickest method to locate medical libraries is to use the Internet-based directory published by the National Network of Libraries of Medicine (NN/LM). It is analogous to bacterial transformation. [NIH] Veterinary Medicine: The medical science concerned with the prevention, diagnosis, and treatment of diseases in animals. It is composed of two parts: DIRLINE and Health Hotlines. The DIRLINE database comprises some 10,000 records of organizations, research centers, and ...
General Information: This is a serogroup A strain isolated in Gambia in 1983. Causes septicemia and meningitis. The second of two pathogenic Neisseria, this organism causes septicemia and is the leading cause of life-threatening meningitis (inflammation of the meninges, the membrane surrounding the brain and spinal cord) in children. This organism typically residies in the nasopharynx cavity but can invade the respiratory epthelial barrier, cross into the bloodstream and the blood brain barrier, and cause inflammation of the meninges. Pathogenicity factors include the surface proteins (porins and opacity proteins), and the type IV pilus (which is also found in Neisseria gonorrhoeae). Pathogenicity factors include the surface proteins (porins and opacity proteins), and the type IV pilus (which is also found in Neisseria gonorrhoeae). This organism, like Neisseria gonorrhoeae, is naturally competent, and protein complexes at the cell surface recognize the uptake signal sequence in extracellular ...
© 2015 Plackett et al. Background: The inability to genetically transform any fern species has been a major technical barrier to unlocking fern biology. Initial attempts to overcome this limitation were based on transient transformation approaches or achieved very low efficiencies. A highly efficient method of stable transformation was recently reported using the fern Ceratopteris richardii, in which particle bombardment of callus tissue achieved transformation efficiencies of up to 72%. As such, this transformation method represents a highly desirable research tool for groups wishing to undertake fern genetic analysis. Results: We detail an updated and optimized protocol for transformation of C. richardii by particle bombardment, including all necessary ancillary protocols for successful growth and propagation of this species in a laboratory environment. The C. richardii lifecycle comprises separate, free-living gametophyte and sporophyte stages. Callus is induced from the sporophyte apex through

Bacterial Transformation: PASCOBacterial Transformation: PASCO

Bacterial Transformation. Lab Summary. Use the tools of biotechnology to transform competent E.coli cells and then select for ... Home / Products / Lab Manuals / College Biology Instructor Guide / Bacterial Transformation. Featured in PASCOs College ... Investigate transformation as a mechanism of genetic exchange.. *Create competent E. coli cells by chemically and thermally ... Calculate the efficiency of transformation.. Method. Students will gain experience conducting the following procedures: * ...
more infohttps://www.pasco.com/products/experiments/college-biology/bacterial-transformation.cfm

Bacterial TransformationBacterial Transformation

Transformation: Occurs when a cell takes up a new piece of DNA and expresses that DNA. [Content Standard C- The cell] ... 4. The transformation kits are [email protected] Genes (pieces of DNA) are responsible for the expression of traits -- Example, eye ... Motivation: 1. Posters of actual transformation. 2. The idea of actually growing bacteria -- Most of the students think of ... 3. They can make these bacterial colonies to Ashow them the [email protected] -- to glow in UV light. ...
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Role of a Deoxyribonuclease in Bacterial Transformation | SpringerLinkRole of a Deoxyribonuclease in Bacterial Transformation | SpringerLink

In the genetic transformation of Diplococcus pneumoniae, donor DNA is converted to single strands either during or just after ... Genetic Transformation Brookhaven National Laboratory DNase Activity Defective Mutant Bacterial Transformation This is a ... Lacks S. (1974) Role of a Deoxyribonuclease in Bacterial Transformation. In: Grell R.F. (eds) Mechanisms in Recombination. ... Molecular fate of DNA in genetic transformation of pneumococcus. J. Mol. Biol. 5: 119.PubMedCrossRefGoogle Scholar ...
more infohttps://link.springer.com/chapter/10.1007/978-1-4684-2133-0_20

Biotechnology: Bacterial Transformation Lab by Ezra Hall on PreziBiotechnology: Bacterial Transformation Lab by Ezra Hall on Prezi

Transcript of Biotechnology: Bacterial Transformation Lab. Results. We expected to see bacterial growth on the LB/amp pGLO ... Bacterial transformation occurs when bacterium absorbs a naked DNA located on there surface and integrates it into there own ... Biotechnology: Bacterial Transformation Lab. Discussion. Our experiment yielded results that were unexpected. One thing that we ... Plasmids are usually used in bacterial transformation because they are small circular DNA molecules that are easily ...
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Bacterial transformation problems - Molecular BiologyBacterial transformation problems - Molecular Biology

Bacterial transformation problems - (Oct/11/2013 ). Im having trouble ligating my insert (Pyp1 promoter region, 1.6kb) into my ... For the bacterial transformation I added 100ul competent E. coli cells to the whole ligation mix (vector, and vector+insert 1:1 ... however when the bacterial transformations didnt produce any colonies, I decided to try digesting with each enzyme separately ... I managed to make this plasmid using the same bacterial transformation procedure as I am using now (transforming Pyp1C20S into ...
more infohttp://www.protocol-online.org/biology-forums-2/posts/30679.html

Bacterial Transformation Kits - MP BiomedicalsBacterial Transformation Kits - MP Biomedicals

Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
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Difference between revisions of Bacterial transformation - OpenWetWareDifference between revisions of "Bacterial transformation" - OpenWetWare

OpenWetWare already has a number of protocols relating to bacterial transformation but more are always welcome. If you plan on ... Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which ... In general, chemical transformation is less prone to error and faster however electroporation produces a higher transformation ... Non-competent cells can be made competent and then transformed via one of two main approaches; chemical transformation and ...
more infohttps://openwetware.org/wiki/?title=Bacterial_transformation&diff=35657&oldid=35557

pGLO™ Bacterial Transformation Kit | Life Science Education | Bio-RadpGLO™ Bacterial Transformation Kit | Life Science Education | Bio-Rad

Demonstrate the power of genetic transformation! Students will glow with excitement when they transform bacteria with pGLO ... 1660003edu-pglo-bacterial-transformation-kit. 1. 1660003EDU. pGLO Bacterial Transformation Kit. pGLO™ Bacterial Transformation ... LSE/pGLO-GFP-Kits/pGLO-Bacterial-Transformation-. N. 0. ,p,Use the pGLO™ Bacterial Transformation Kit Instruction Manual with ... 1660033edu-pglo-bacterial-transformation-kit-instruction-manual. 1. 1660033EDU. pGLO Bacterial Transformation Instruction ...
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Bacterial Transformation - Educational ProductsBacterial Transformation - Educational Products

Bacterial Transformation. Bacterial transformation allows researchers to insert their recombinant DNA into bacteria, which then ... Bacterial Transformation For 6 groups of 4-5 or 24-30 students. $264.00 ... Students will explore the principles of bacterial transformation and will learn how to make bacteria susceptible, or competent ... The results of the transformation can be easily visualized under UV light as glowing bacteria. ...
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pGLO™ Bacterial Transformation Kit | Life Science Education | Bio-RadpGLO™ Bacterial Transformation Kit | Life Science Education | Bio-Rad

Demonstrate the power of genetic transformation! Students will glow with excitement when they transform bacteria with pGLO ... 1660003edu-pglo-bacterial-transformation-kit. 1. 1660003EDU. pGLO Bacterial Transformation Kit. pGLO™ Bacterial Transformation ... LSE/pGLO-GFP-Kits/pGLO-Bacterial-Transformation-. N. 0. ,p,Use the pGLO™ Bacterial Transformation Kit Instruction Manual with ... 1660033edu-pglo-bacterial-transformation-kit-instruction-manual. 1. 1660033EDU. pGLO Bacterial Transformation Instruction ...
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Lab Skills Nights! - Bacterial Transformation of Plasmid DNA Tickets, Thu, Jul 25, 2019 at 7:00 PM | EventbriteLab Skills Nights! - Bacterial Transformation of Plasmid DNA Tickets, Thu, Jul 25, 2019 at 7:00 PM | Eventbrite

Bacterial Transformation of Plasmid DNA - Thursday, July 25, 2019 at Baltimore Under Ground Science Space, Baltimore, MD. Find ... Lab Skills Nights! - Bacterial Transformation of Plasmid DNA. by Baltimore Under Ground Science Space ... Lab Skills Nights! - Bacterial Transformation of Plasmid DNA at Baltimore Under Ground Science Space 101 North Haven Street # ... The month of July we will be offering Bacterial Transformation of Plasmid DNA! ...
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Bacterial Transformation: Laboratory Experiment Lesson Plan for 9th - 10th Grade | Lesson PlanetBacterial Transformation: Laboratory Experiment Lesson Plan for 9th - 10th Grade | Lesson Planet

Students participate in a group lab in which they complete the process of bacterial transformation. If lab procedures are ... This Bacterial Transformation: Laboratory Experiment Lesson Plan is suitable for 9th - 10th Grade. ... This Bacterial Transformation: Laboratory Experiment lesson plan also includes:. * Worksheet * Join to access all included ... Students participate in a group lab in which they complete the process of bacterial transformation. If lab procedures are ...
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Teachers Guide: Bacterial TransformationTeachers Guide: Bacterial Transformation

... Probably the greatest strength of molecular biology is the ability to manipulate ... Transformation involves mixing competent bacteria with plasmid DNA and then selecting bacteria containing the plasmid using ... This process is called transformation. Bacteria are treated so they will take the plasmid up into their cells. These are called ... This laboratory protocol will allow students to demonstrate the phenomenon of transformation in a relatively simple procedure. ...
more infohttp://www.sci-ed-ga.org/teachers-guide-bacterial-transformation

Teach bacterial transformation and the use of plasmids in genetic engineeringTeach bacterial transformation and the use of plasmids in genetic engineering

... competent cells and bacterial transformation. A complete hands-on teaching kit to introduce students to genetic engineering. ... The second, BE-309 "Bacterial Transformation" is a hands-on teaching kit from the BioScience Excellence™ program by G- ... competent cells and bacterial transformation providing an excellent introduction to genetic engineering. ... Easily detect succesful transformation with supplied antibiotic screening and visualization of the plasmid under UV light. ...
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Teach bacterial transformation and the use of plasmids in genetic engineeringTeach bacterial transformation and the use of plasmids in genetic engineering

... competent cells and bacterial transformation. A complete hands-on teaching kit to introduce students to genetic engineering. ... The second, BE-309 "Bacterial Transformation" is a hands-on teaching kit from the BioScience Excellence™ program by G- ... competent cells and bacterial transformation providing an excellent introduction to genetic engineering. ... Easily detect succesful transformation with supplied antibiotic screening and visualization of the plasmid under UV light. ...
more infohttps://www.gbiosciences.com/Educational-Products/BBED-8H_GFP-Genetic-Engineering-of-Bacteria

How to perform a Bacterial Transformation - DnaTube.com - Scientific Video and Animation SiteHow to perform a Bacterial Transformation - DnaTube.com - Scientific Video and Animation Site

Bacterial transformation is process where one forces bacteria to uptake foreign DNA and to built it up in its own genome. If ... This video is shooting of protocol for bacterial transformation. ... Tags: Bacterial Transformation Lab Uploaded by: bresca ( Send ... How to perform a Bacterial Transformation This video is shooting of protocol for bacterial transformation. Bacterial ... If transformation was successful, bacteria with new ability will grow. Here it is used ability to be resistant to ampicillin, ...
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Bacterial natural transformation by highly fragmented and damaged DNA | PNASBacterial natural transformation by highly fragmented and damaged DNA | PNAS

Bacterial natural transformation by degraded DNA. Søren Overballe-Petersen, Klaus Harms, Ludovic A. A. Orlando, J. Victor ... Bacterial natural transformation by degraded DNA. Søren Overballe-Petersen, Klaus Harms, Ludovic A. A. Orlando, J. Victor ... Bacterial natural transformation by highly fragmented and damaged DNA Message Subject (Your Name) has sent you a message from ... Bacterial natural transformation by highly fragmented and damaged DNA. Søren Overballe-Petersen, Klaus Harms, Ludovic A. A. ...
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Arabidopsis Transformation with Large Bacterial Artificial Chromosomes | SpringerLinkArabidopsis Transformation with Large Bacterial Artificial Chromosomes | SpringerLink

Alonso J.M., Stepanova A.N. (2014) Arabidopsis Transformation with Large Bacterial Artificial Chromosomes. In: Sanchez-Serrano ... TAC Transformation Arabidopsis T-DNA DNA deletions Electroporation Agrobacterium This is a preview of subscription content, log ... Chang Y-C, Henriquez XH, Preuss DP, Copenhaver GC, Zhang HZ (2003) A plant-transformation-competent BIBAC library from the ... Sheng Y, Mancino V, Birren B (1995) Transformation of Escherichia coli with large DNA molecules by electroporation. Nucleic ...
more infohttps://link.springer.com/protocol/10.1007%2F978-1-62703-580-4_15

Transformation, Bacterial - Semantic ScholarTransformation, Bacterial - Semantic Scholar

Transformation, Bacterial. Known as: Bacterial transformation The heritable modification of the properties of a competent ... Improvement of bacterial transformation efficiency using plasmid artificial modification. *Kazumasa Yasui, Yasunobu Kano, +4 ... Adenosine 3:5-cyclic monophosphate as a regulator of bacterial transformation.. *Edmund Merriman Wise, Susan Alexander, M B ... Bacterial transformation, in which cells take up and recombine free strands of DNA, is the simplest process thought to have… ( ...
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Essay about Bacterial Transformation - 2346 WordsEssay about Bacterial Transformation - 2346 Words

Essay on Bacterial Transformation Using pGLO Involving X and Y Genes ... Bacterial Transformation using pGLO involving X and Y ... Introduction Bacterial transformation is the permanent alteration of a bacterial cell genotype as a result of its uptake and ... Bacterial transformation has a lot of uses such as mapping bacterial chromosomes (Anthony et al, 2008). This experiment is ... Genetic transformation is where one organism takes on a characteristic from another organism (Bacterial Transformation 2013). ...
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IJMS | Free Full-Text | Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida EffectIJMS | Free Full-Text | Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect

In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in ... Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism ... which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be ... previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. ...
more infohttp://www.mdpi.com/1422-0067/11/12/4962

Biological Physics: Bacterial transformationBiological Physics: Bacterial transformation

Illustration of bacterial transformation. Bacteria import DNA from the environment and integrate the newly acquired DNA by ... The simplest mechanism for gene transfer is called transformation. Transformation is the import and inheritable integration of ... Since the gene encoding for eyfp is integrated into the chromosome, the offspring of the initial transformation are also red ... Transformation enables bacteria to acquire parts of genes, entire genes, and even operons. ...
more infohttp://www.biophysics.uni-koeln.de/12762.html

Bacterial Transformation Post lab Questions: - PDFBacterial Transformation Post lab Questions: - PDF

Bacterial Transformation Post lab Questions: 1. This graph represents typical bacteria growth and death on any culture plate. ... Lab 9: Bacterial Transformation with pglo Name: Lab 9: Bacterial Transformation with pglo OBJECTIVES: ο Practice formulating ... Genetic transformation literally means change caused by genes. pglo Bacterial Transformation Practical What is transformation? ... Bacterial Transformation with Green Fluorescent Protein. Table of Contents Fall 2012 Bacterial Transformation with Green ...
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Bacterial Transformation Protocol with Competent Cells - SciGineBacterial Transformation Protocol with Competent Cells - SciGine

Enjoy a protocol guide: Bacterial Transformation using Competent Cells and Plasmid DNA. ... Transformation is the process of introducing new DNA into bacteria. ... Bacterial Transformation Video Tutorial. Applications of DNA Transformation on Scigine. *Bacterial Transformation with pORFES ... Excellent Book about Bacterial Transformation. Guide to Common terms in Transformation - Oklahoma University. Compilation of ...
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  • Our experiment yielded some unexpected results as we discovered no growth on any of the plates except for the LB pGLO - plate which had approximately 320 small bacterial colonies growing on it. (prezi.com)
  • The presentation provides educators with background on the pGLO Transformation and Green Fluorescent Protein Chromatography Kits and includes instructions for using the kits. (bio-rad.com)
  • The simplest mechanism for gene transfer is called transformation. (uni-koeln.de)
  • Since the gene encoding for eyfp is integrated into the chromosome, the offspring of the initial transformation are also red fluorescent. (uni-koeln.de)
  • Another key challenge is that the transformation process may lead to some DNA being recombined so that your gene of interest is no longer functional. (scigine.com)
  • Explanation: Molecular weights of DNA in the range of 300,000 to 8 million daltons have been shown to result in successful transformation. (sanfoundry.com)