A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Gene Transfer Techniques
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
A muscle protein localized in surface membranes which is the product of the Duchenne/Becker muscular dystrophy gene. Individuals with Duchenne muscular dystrophy usually lack dystrophin completely while those with Becker muscular dystrophy have dystrophin of an altered size. It shares features with other cytoskeletal proteins such as SPECTRIN and alpha-actinin but the precise function of dystrophin is not clear. One possible role might be to preserve the integrity and alignment of the plasma membrane to the myofibrils during muscle contraction and relaxation. MW 400 kDa.
Mice, Inbred mdx
A strain of mice arising from a spontaneous MUTATION (mdx) in inbred C57BL mice. This mutation is X chromosome-linked and produces viable homozygous animals that lack the muscle protein DYSTROPHIN, have high serum levels of muscle ENZYMES, and possess histological lesions similar to human MUSCULAR DYSTROPHY. The histological features, linkage, and map position of mdx make these mice a worthy animal model of DUCHENNE MUSCULAR DYSTROPHY.
Muscular Dystrophy, Duchenne
An X-linked recessive muscle disease caused by an inability to synthesize DYSTROPHIN, which is involved with maintaining the integrity of the sarcolemma. Muscle fibers undergo a process that features degeneration and regeneration. Clinical manifestations include proximal weakness in the first few years of life, pseudohypertrophy, cardiomyopathy (see MYOCARDIAL DISEASES), and an increased incidence of impaired mentation. Becker muscular dystrophy is a closely related condition featuring a later onset of disease (usually adolescence) and a slowly progressive course. (Adams et al., Principles of Neurology, 6th ed, p1415)
Gene Transfer, Horizontal
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Killer Cells, Natural
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
Allergy and Immunology
Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.
Small, monomeric GTP-binding proteins encoded by ras genes (GENES, RAS). The protooncogene-derived protein, PROTO-ONCOGENE PROTEIN P21(RAS), plays a role in normal cellular growth, differentiation and development. The oncogene-derived protein (ONCOGENE PROTEIN P21(RAS)) can play a role in aberrant cellular regulation during neoplastic cell transformation (CELL TRANSFORMATION, NEOPLASTIC). This enzyme was formerly listed as EC 18.104.22.168.
Bioluminescence Resonance Energy Transfer Techniques
A G-protein-coupled, proteinase-activated receptor that is expressed in a variety of tissues including ENDOTHELIUM; LEUKOCYTES; and the GASTROINTESTINAL TRACT. The receptor is activated by TRYPSIN, which cleaves off the N-terminal peptide from the receptor. The new N-terminal peptide is a cryptic ligand for the receptor. The uncleaved receptor can also be activated by the N-terminal peptide present on the activated THROMBIN RECEPTOR and by small synthetic peptides that contain the unmasked N-terminal sequence.
Fluorescence Resonance Energy Transfer
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
RNA Processing, Post-Transcriptional
A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
A generic name for film produced from wood pulp by the viscose process. It is a thin, transparent sheeting of regenerated cellulose, moisture-proof and sometimes dyed, and used chiefly as food wrapping or as bags for dialysis. (Grant & Hackh's Chemical Dictionary, 5th ed & McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Peptide Nucleic Acids
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
"Gene transfer (transfection) methods in animals , Genetic Engineering and Biotechnology Gene Transfer Methods and Transgenic ... The bacteria uses conjugation to transfer a DNA segment called T-DNA from its plasmid into the plant. The transferred DNA is ... Chemical based methods of gene delivery can use natural or synthetic compounds to form particles that facilitate the transfer ... Joos H, Timmerman B, Montagu MV, Schell J (1983). "Genetic analysis of transfer and stabilization of Agrobacterium DNA in plant ...
Genetic engineering techniques
"Gene transfer (transfection) methods in animals , Genetic Engineering and Biotechnology Gene Transfer Methods and Transgenic ... The bacteria uses conjugation to transfer a DNA segment called T-DNA from its plasmid into the plant. The transferred DNA is ... Another method is Embryonic Stem Cell-Mediated Gene Transfer. The gene is transfected into embryonic stem cells and then they ... Genetically modified viruses can be used as viral vectors to transfer target genes to another organism in gene therapy. First ...
Current transfection efficiency remains poor. One example of the successful use of L. monocytogenes in in vitro transfer ... "Listeria monocytogenes mediated CFTR transgene transfer to mammalian cells". The Journal of Gene Medicine. 4 (6): 655-67. doi: ...
Methods for transferring foreign genetic material into a recipient cell include transfection and transduction. The choice of ... The genetic material that is transferred typically must be within a format that encourages the recipient cell to express the ... Heterologous (meaning 'derived from a different organism') refers to the fact that often the transferred protein was initially ... Typically the protein itself is not transferred, but instead the 'correctly edited' genetic material coding for the protein ( ...
Cell line creation involves transfection (transferring the gene into the cell line), and selection (applying selective pressure ... Transfection efficiency is dependent on cell type, cell density, vector, and transfection method. Selection efficiency depends ... Brielmeier, M; Béchet, JM; Falk, MH; Pawlita, M; Polack, A; Bornkamm, GW (1 May 1998). "Improving stable transfection ...
The introduction of exogenous DNA into a cell is called transformation (transfection in animal cells). This can take place ... DEAE-dextran complexation and lipid-mediated DNA transfer; (b) physical methods, including electroporation, microinjection, and ... Methods of artificial transfection include (a) chemical methods, including calcium phosphate precipitation, ...
Synthesis by Group Transfer Polymerization, Characterization and Evaluation as Transfection Reagents". Biomacromolecules. 5 (6 ... For some polymers it was demonstrated that thermoresponsive behavior can be transferred to surfaces. The surface is either ...
Lower critical solution temperature
Synthesis by Group Transfer Polymerization, Characterization and Evaluation as Transfection Reagents". Biomacromolecules. 5 (6 ...
Because of this, electroporation is one of the key methods of transfection as well as bacterial transformation. It has even ... Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982). "Gene transfer into mouse lyoma cells by electroporation in high ... Flippases are members of a larger family of lipid transport molecules that also includes floppases, which transfer lipids in ... August 2001). "Laboratory-scale evidence for lightning-mediated gene transfer in soil". Appl. Environ. Microbiol. 67 (8): 3440- ...
In contrast, other commonly used non-viral transfection methods rely on cell division for the transfer of DNA into the nucleus ... Nucleofection is an electroporation-based transfection method which enables transfer of nucleic acids such as DNA and RNA into ... The non-viral gene transfer methods available were not suitable for the efficient transfection of primary cells. Non-viral ... Nucleofection is a method to transfer substrates into mammalian cells so far considered difficult or even impossible to ...
Induced pluripotent stem cell
Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). Since iPSCs can be ... The Yamanaka group successfully reprogrammed mouse cells by transfection with two plasmid constructs carrying the reprogramming ... In June 2014, Takara Bio received technology transfer from iHeart Japan, a venture company from Kyoto University's iPS Cell ... Epigenetic reprogramming Promoter demethylation: Methylation is the transfer of a methyl group to a DNA base, typically the ...
"Polyplus transfection". CNRS. "Les quinze lauréats de la Médaille d'argent du CNRS 1998". cnrs.fr. Archived from the original ... 86 (18), p. 6982-6986 Boussif, O, « A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo ... These vectors are widely used as transfection agents for animal cells in culture, but also as drug-gene carriers in clinical ... 27, p. 5861-5864 Behr, J.P., « Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine- coated DNA ...
Bacterial artificial chromosome
They are transferred over to these genetic disease models by electroporation/transformation, transfection with a suitable virus ... These constructs are referred to as "infectious clones", as transfection of the BAC construct into host cells is sufficient to ...
This could result in a PAMAM-DNA complex, which would make DNA transfection more efficient due to neutralization of the charges ... In the context of existing approaches to gene transfer, PAMAM dendrimers hold a strong position relative to major classical ... As with drug delivery applications, specific targeting of the transfection complex is ideal and must be explored as well. ... Kukowska-Latallo JF, Bielinska AU, Johnson J, Spindler R, Tomalia DA, Baker JR (May 1996). "Efficient transfer of genetic ...
The purpose of this method is to transfer the droplet from a lower planar surface to an upper parallel planar surface and back ... PCR amplified products are then validated by transfection into yeast cells and a Western blot protein identification. Problems ... In one application, microbial cultures have been transferred onto the chip and allowed to grow with the use of sterile ... Application of an electric potential allows for automation of droplet transfer directly to the hanging cell culture.] This is ...
An oocyte can reprogram an adult nucleus into an embryonic state after somatic cell nuclear transfer, so that a new organism ... It is carried out by the transfection of stem-cell associated genes into mature cells using viral vectors such as retroviruses ... During somatic cell nuclear transfer, the oocyte turns off tissue specific genes in the Somatic cell nucleus and turns back on ... Yamanaka was the first to demonstrate (in 2006) that this somatic cell nuclear transfer or oocyte-based reprogramming process ( ...
Vectors in gene therapy
Replication-defective vectors always contain a "transfer construct". The transfer construct carries the gene to be transduced ... The use of particle bombardment, or the gene gun, is another physical method of DNA transfection. In this technique, DNA is ... This is mostly examined for gene transfer in the nervous system. The wild type HSV-1 virus is able to infect neurons and evade ... The most common use of lipoplexes has been in gene transfer into cancer cells, where the supplied genes have activated tumor ...
SR-B1 mediated delivery has also been used in the transfection of cancer cells with siRNA, or small interfering RNAs. This ... While SR-B1 normally mediates the transfer of cholesterol between high-density lipoproteins (HDL) and healthy cells, it also ...
An example is the viral transfer of DNA from one bacterium to another and hence an example of horizontal gene transfer. ... Signal transduction Transfection - means of inserting DNA into a cell. Transformation (genetics) - means of inserting DNA into ... insertion of the DNA to be transferred into the cellular genome. However, since the transferred genetic material does not ... In these cases, a plasmid is constructed in which the genes to be transferred are flanked by viral sequences that are used by ...
The challenge has always been how to deliver the siRNA using a topical method or retroviral vectors and ex vivo gene transfer. ... and do not require barrier disruption or transfection agents, such as liposomes, peptides, or viruses. "Topical application of ...
Vector (molecular biology)
The purpose of a vector which transfers genetic information to another cell is typically to isolate, multiply, or express the ... Insertion of a vector into the target cell is usually called transformation for bacterial cells, transfection for eukaryotic ... Such vectors have bacterial or viral elements which may be transferred to the non-bacterial host organism, however other ... ISBN 978-0-471-45329-1. Waksman Scholars introduction to vectors A comparison of vectors in use for clinical gene transfer Gene ...
The complete mtDNA genome or just fragments of PCR-generated mtDNA can be transferred into the target mitochondria through the ... Protofection is a protein-mediated transfection of foreign mitochondrial DNA (mtDNA) into the mitochondria of cells in a tissue ...
Förster resonance energy transfer
Heitz M, Zamolo S, Javor S, Reymond JL (June 2020). "Fluorescent Peptide Dendrimers for siRNA Transfection: Tracking pH ... Dexter electron transfer Förster coupling Surface energy transfer Time-resolved fluorescence energy transfer Cheng P (2006). " ... Förster or fluorescence resonance energy transfer (FRET), resonance energy transfer (RET) or electronic energy transfer (EET) ... "Förster resonance energy transfer" is preferred to "fluorescence resonance energy transfer"; however, the latter enjoys common ...
August 2005). "Lentiviral transfection with the PDGF-B gene improves diabetic wound healing". Plast. Reconstr. Surg. 116 (2): ... "HIV-2 and SIV Vector Systems." Lentiviral Vector Systems for Gene Transfer. Ed. Gary L. Buchschacher. Georgetown, TX: Eurekah. ...
Single cell transfections are used to virtually transfer any type of mammalian cell into another using a syringe which creates ... This is important because it allows for tip penetration into the cytosol, which is critical for viable DNA transfer into the ... Although advances in gene transfer technology, including viral and non-viral vectors, have been made, an ideal vector system ... Mehierhumbert, S.; Guy, R. (2005-04-05). "Physical methods for gene transfer: Improving the kinetics of gene delivery into ...
was acquired by Molecular Transfer, Inc. in 2009. In 2015, the newly organized company was rebranded as MTI-GlobalStem. MTI- ... human induced pluripotent stem cells and culture reagents Transfection and Protein production Tools Characterization services " ...
Potter, H (2003). "Transfection by Electroporation". Current Protocols in Molecular Biology. Arena CB, Sano MB, Rossmeisl JH, ... Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982). "Gene transfer into mouse lyoma cells by electroporation in high ... Electroporation has proven efficient for use on tissues in vivo, for in utero applications as well as in ovo transfection. ... Particularly, the electroporation allows for a more efficient transfection of DNA, RNA, shRNA, and all nucleic acids into the ...
... gene transfer technique that has gained widespread use because of advantages when compared to other transfection methods, (for ... Baculovirus gene transfer into Mammalian cells, known from scientific research articles as BacMam, is the use of baculovirus to ... Bisafety and viral gene transfer vectors. In Biological safety, principles and practices, 3rd ed. D. O. Fleming and D. L. Hunt ... Boyce, FM; Bucher, NL (1996). "Baculovirus-mediated gene transfer into mammalian cells". Proceedings of the National Academy of ...
... then they are transferred to a cell culture in the process called transfection and once the cell culture produces the desired ...
The purified minicircle can be transferred into the recipient cell by transfection or lipofection and into a differentiated ... The Future of Nonviral and Viral Gene Transfer. Wiley‐VCH Verlag GmbH & Co. KGaA. pp. 115-162. doi:10.1002/9783527670420.ch8. ...
High Efficiency Gene Transfer by Multiple Transfection Protocol | SpringerLink
Using LipofectAMINE, a widely used transfection reagent, we transfected 293T cells with a plasmid harboring... ... A high efficiency transfection protocol employing a common polycationic lipid is described. ... Lipid Gene Transfer 293T Cell Transfection Efficiency Transfection Reagent These keywords were added by machine and not by the ... The transfection efficiency was determined by direct staining for X-gal. The conventional transfection protocol achieved an ...
Neuronal transfection in brain slices using particle-mediated gene transfer. - PubMed - NCBI
Neuronal transfection in brain slices using particle-mediated gene transfer.. Lo DC1, McAllister AK, Katz LC. ... We describe here an alternative strategy using particle-mediated gene transfer for the transfection of neurons and glia in ... Conventional transfection techniques have been of limited effectiveness, particularly in intact neural tissues. Viral vectors ... Difficulties in neuronal transfection continue to restrict the applicability of molecular approaches to neurobiology. ...
Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection - Infoscience
We also demonstrate that expression-enhancing treatment with glycerol during transfection did not result in increased DNA ... Analyses by flow cytometry and confocal microscopy have also supported results suggesting endocytosis during Ca-Pi transfection ... but also that DNA transfer into cells is a dynamic process that follows a defined kinetics of uptake and intracellular ... Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection Batard, P. ; Jordan, M. ; Wurm, F. ...
Differential effects of STAT5 and PI3K/AKT signaling on effector and memory CD8 T-cell survival | PNAS
D) CA-AKT (blue bar) or CTRL (white bar) RV+ (Thy1.1+) P14 CD8 T cells from day 15 post-LCMV infection was transferred into ... Retroviral Transfection.. Activated P14 TCR transgenic T cells were transfected with murine stem cell virus retroviruses ... 2, P14 CD8 T cells were transduced with CA-AKT or CTRL RVs, and ∼2,500 cells were transferred into C57BL/6 mice that were ... 4, P14 CD8 T cells were transduced with CA-STAT5 or CTRL RVs and ∼2,500 cells were transferred into C57BL/6 mice that were ...
Local and distant transfection of mdx muscle fibers with dystrophin and LacZ genes delivered in vivo by synthetic microspheres ...
High levels of dystrophin cDNA expression, and an efficient distant transfection effect with preferential intranuclei inclusion ... Coonrod A, Li F-Q, Horwitz M . On the mechanism of DNA transfection: efficient gene transfer without viruses Gene Therapy 1997 ... Efficient transfection of muscle nuclei with dystrophin cDNA in combination with a clearcut distant transfection effect and ... Distant transfection phenomena studies. Transfection of contralateral muscles after a single administration of human dystrophin ...
Gene Transfer to Mammalian Cells - transfection & transduction 레포트
따라서 mammalian cell에서는 특별히 transfection이라고 한다. 먼저 transfection은 mammalian cell에 외부 유전자를 DNA 형태로 도입하는 방.. ... 따라서 mammalian cell에서는 특별히 transfection이라고 한다. 먼저 transfection은 mammalian cell에 외부 유전자를 DNA 형태로 도입하는 방법이다. DNA를 culture 중인 cell ... 따라서 DNA를 cell 속으로, 더구나 핵 속으로 까지 유입 시키는 것은 여러 가지 특별한 agent를 통한 transfection method를 사용해야 하는 까다로운 일이며, 높은 tranfection efficiency를 ... 이번 실험에서는 외부 gene을 mammalian cell 내로 옮겨 발현시키는 여러 가지 방법 중 transfection과 transduction에 대해
Bead transfection of adherent cells. Gene transfer into adhere...
Gene transfer into adherent mammalian cells using glass beads.: Bead transfection is a simple, rapid, efficient, and cost-e ... Bead transfection of adherent cells. Gene transfer into adherent mammalian cells using glass beads.. Authors * Matthews, K E ... Bead transfection is a simple, rapid, efficient, and cost-effective method of gene transfer into adherent mammalian cells. It ... As this technique delivers high transfection efficiency with little manipulation of the exogenous DNA and does not require the ...
ATM controls DNA repair and mitochondria transfer between neighboring cells | SpringerLink
Plasmid DNA transfection. Cells were plated onto uncoated 35 mm dishes with a 0.17 mm glass bottom (MatTek) and allowed to ... a-f Mitochondria transfer in ATMwt and ATM−/− fibroblasts upon irradiation. Mitochondria transfer was monitored between donor ... Nuclear genome transfer in human oocytes eliminates mitochondrial DNA variants. Nature. 2013;493:632-7.CrossRefGoogle Scholar ... Transfer of mitochondria via tunneling nanotubes rescues apoptotic PC12 cells. Cell Death Differ. 2015;22:1181-91.CrossRef ...
Ras Oncoproteins Transfer from Melanoma Cells to T Cells and Modulate Their Effector Functions | The Journal of Immunology
Plasmids and transfection. The expression vectors containing cDNA encoding GFP, GFP-H-RasG12V, were previously described (8). ... We also found that the transfer of GFP-H-RasG12V to TILs was substantial by 30 min and moreover the degree of transfer ... Cocultures and FACS analysis of intercellular transfer. To examine the intercellular transfer of GFP-tagged molecules, the ... various aspects of the transfer of information from cancer to immune cells and how this transfer regulates immunoediting of ...
Frontiers | Interaction of Protease-Activated Receptor 2 with G Proteins and β-Arrestin 1 Studied by Bioluminescence Resonance...
Here, we applied bioluminescence resonance energy transfer (BRET) to shed more light on the G protein coupling profile of ... Here, we applied bioluminescence resonance energy transfer (BRET) to shed more light on the G protein coupling profile of ... Transient transfections were performed by reverse transfection in 96-well plate using Lipofectamine 2000 following the ... Cell Culture and Transfection. COS-7 cells were grown in complete medium [DMEM supplemented with 10% (v/v) fetal bovine serum, ...
Regulation of Microsomal Triglyceride Transfer Protein Gene by Insulin in HepG2 Cells | Diabetes
Transfection and reporter assay.. Cells were seeded at ∼70% density in 12-well plates. A DNA/ liposome mix containing 2 μg of ... Regulation of Microsomal Triglyceride Transfer Protein Gene by Insulin in HepG2 Cells. Roles of MAPKerk and MAPKp38. Wo-Shing ... Transfections were allowed to proceed for 6 h in the absence of serum, and the cells were recovered in medium supplemented with ... Microsomal triglyceride transfer protein (MTP) is rate limiting for the assembly and secretion of apolipoprotein B-containing ...
Comparison of transfection efficiency of nonviral gene transfer reagents<...
Comparison of transfection efficiency of nonviral gene transfer reagents. / Yamano, Seiichi; Dai, Jisen; Moursi, Amr M. ... Yamano, Seiichi ; Dai, Jisen ; Moursi, Amr M. / Comparison of transfection efficiency of nonviral gene transfer reagents. In: ... Yamano, S., Dai, J., & Moursi, A. M. (2010). Comparison of transfection efficiency of nonviral gene transfer reagents. ... Comparison of transfection efficiency of nonviral gene transfer reagents. Molecular Biotechnology. 2010 Nov;46(3):287-300. ...
High-throughput screening of clinically approved drugs that prime polyethylenimine transfection reveals modulation of...
... drugs that prime polyethylenimine transfection reveals modulation of mitochondria dysfunction response improves gene transfer ... To develop a library of transfection priming compounds, a high-throughput screen was performed of the NIH Clinical Collection ( ... Fold-changes for each compound were linked to drug clusters, from which drug classes that prime transfection were identified. ... In addition to modifications to nucleic acid design and nonviral carriers, new tools are sought to enhance transfection. ...
Mast cells and dendritic cells form synapses that facilitate antigen transfer for T cell activation | JCB
Transfection. BMMCs were transfected with either GPI-GFP or CD9-GFP expression plasmids (both are the pEGFP backbone with ... This material transfer was rapid, occurring within 30 min of MC activation. Direct contact was required for material transfer, ... Quantitation of material transfer and cellular polarization. To determine DCs that contained material transferred from ... where transfer occurs rapidly at the actMC-imDC synapse. Transferred material is found within DC endosomal compartments as ...
Patent US20030228601 - Transfection method and uses related thereto - Google Patents
6 shows a transfection array that has been transferred to a nitrocellulose filter. ... Transfers can be performed so that 1) the entire cellular material on the microarray is transferred (i.e. both the endogenous ... Reverse transfection method. US6951757 *. Mar 4, 2003. Oct 4, 2005. Whitehead Institute For Biomedical Research. Transfection ... in order to promote co-transfection of the host cells with at least two different target sequences. Co-transfection refers to ...
Adenovirus-mediated Transfer of Inducible Caspases | Cancer Research
Transfections/Luciferase Assay.. Human prostate cell lines, PC-3, DU145, and TSU-Prl, were plated at 1 × 105 cells/35-mm tissue ... Adenovirus-mediated Transfer of Inducible Caspases. Shahrokh F. Shariat, Smruti Desai, Weitao Song, Tahira Khan, Julie Zhao, ... Adenovirus-mediated Transfer of Inducible Caspases. Shahrokh F. Shariat, Smruti Desai, Weitao Song, Tahira Khan, Julie Zhao, ... Adenovirus-mediated Transfer of Inducible Caspases Message Subject (Your Name) has forwarded a page to you from Cancer Research ...
A heterotypic bystander effect for tumor cell killing after adeno-associated virus/phage-mediated, vascular-targeted suicide...
Plasmids and Transfections. HSVtk was expressed in endothelial SVEC4-10 cells by transfection of a adeno-associated virus (pAAV ... Gene transfer to mammalian cells using genetically targeted filamentous bacteriophage. FASEB J 1999;13:727-34. ... Suicide gene transfer is the most commonly used cytotoxic approach in cancer gene therapy; however, a successful suicide gene ... It is based on the correction of pathologic gene expression patterns (e.g. by the transfer of tumor suppressor genes) or on the ...
Difference between revisions of "Griffin:shRNA Transfection" - OpenWetWare
shRNA Transfer Vector Transient Transfection Procedure. *In a six well tissue culture plate, grow cells to a 50-70% confluency ... Solution B: For each transfection, dilute 1 - 6 μl of shRNA Plasmid Transfection Reagent with enough shRNA Plasmid Transfection ... thereby reducing the transfection efficiency. For each transfection, add 0.8 ml shRNA Plasmid Transfection Medium to well. ... shRNA Transfer Vector Transfection Reagents. Transient gene silencing using RNAi is critically dependent on highly efficient ...
Suppression of Autoimmune Diabetes by Viral IL-10 Gene Transfer | The Journal of Immunology
High dose transfection with rAAV in vitro did not cause dysfunction of insulin secretion to glucose stimulation in isolated ... Blocking diabetes induction by adoptive splenocyte transfer. Adoptive transfer of splenocytes from diabetic NOD mice induces ... Cumulative incidence of hyperglycemia (%) in NOD recipients after adoptive splenocyte transfer. A, Adoptive transfer ... Suppression of Autoimmune Diabetes by Viral IL-10 Gene Transfer. Zandong Yang, Meng Chen, Runpei Wu, Lawrence B. Fialkow, ...
PULSin - Powerful delivery reagent - Polyplus-transfection
Highly efficient transfer. The comparison of PULSin with two other protein delivery reagents shows a higher efficiency of ... For the first time, achieve superior protein yields using transient transfection and gene expression. FectoPRO® Transfection ... siRNA transfection to brain. Products. Plasmid DNA. Oligo-nucleotides. mRNA. Proteins. Adherent. Suspension. Specific. ... Number of transfection. 0.4 ml of PULSin delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and ...
Patent US6071276 - Cell transfection apparatus and method - Google Patents
... a catheter arrangement with various embodiments for applying heat to a patients cells in vivo in order to improve transfection ... "Gene Transfer Into Mammalian Cells By Rapid Freezing", Kiyoshi Sasaki et al, In Vitro Cell. Dev. Biol. 27A: pp. 86-88, Jan. ... SMC transfection was performed using DNA coprecipitated with CaPO4. SMCs were immediately heated for up to 1 hour at 42 to 45 ... One technique for transfection of cells has used laser poration. This approach has been performed in vitro using a laser beam ...
Luciferase mRNA Transfection of Antigen Presenting Cells Permits Sensitive Nonradioactive Measurement of Cellular and Humoral...
Generation of IVT RNA and Transfer into Cells. IVT RNA was generated as previously described  and added to cells suspended ... 24 h after transfection, 1.2 mg/mL D-luciferin was added and luminescence of different amounts of cells was measured. (e) ... 24 h after transfection, 1.2 mg/mL D-luciferin was added and luminescence of different amount of cells was measured. Cell ... 24 h after transfection, luminescence of 5 × 104 viable or 0.2% Triton X-100 treated cells was repeatedly measured after a ...
Pharmaceutics | Free Full-Text | Exploring the Correlation Between Lipid Packaging in Lipoplexes and Their Transfection...
Of the lipoplexes prepared, those formulated with DSTAP showed higher transfection efficacy, however, the effect of buffer on ... In this research, we investigate if lipid packaging can give further insights into transfection efficacy. DNA lipoplexes ... to prepare the lipoplexes increased the size of vesicles in most of formulations and resulted in variation in transfection ... to investigate the effect of buffer salts on lipoplex physicochemical characteristics and in vitro transfection. In addition, ...
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences
Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system | Cell Death & Disease
In our study, we obtained IAPP gene-humanized miniature pigs via the CRISPR/Cas9 system and somatic cell nuclear transfer (SCNT ... Somatic cell nuclear transfer (SCNT) and embryo transfer (ET). SCNT and ET were performed according to previous research55. ... ① Isolation and culture of miniature PFFs; ② Transfection of miniature PFFs; ③ Inoculate cells into 10 cm culture dishes using ... ⑧ Somatic cell nuclear transfer; ⑨ Embryo transfer; ⑩ Identification of hIAPP cloned pigs. b, c Positive cloned cells were ...
PDLSCs Regulate Angiogenesis of Periodontal Ligaments via VEGF Transferred by Exosomes in Periodontitis
Transfection of miRNA mimics and inhibitor. Cells were used for transfection when reached to 65% confluence. PDLSCs were ... Effect of inflammation on angiogenesis via regulating exosome-transferred VEGF signaling of PDLSCs targeted by miR-17-5p. VEGFA ... The proteins were transferred to PVDF membrane after separation. 5% non-fat milk powder solution was used to block non-specific ... The transfection medium was replaced after 8 h, and the cells were collected for protein analysis after 48 h. ...
Transfer of polyglutamine aggregates in neuronal cells occurs in tunneling nanotubes | Journal of Cell Science
Transfer of GFP-480-68Q aggregates occurs through TNTs in co-cultured CAD cells. 1-day post-transfection, GFP-480-68Q- ... Overall, these data indicate that at 48 hours post-transfection, aggregates formed within a cell are able to transfer to ... Intercellular transfer of Htt aggregates requires cell-cell contact. The transfer of Htt aggregates could have occurred either ... To characterize the transfer mechanism we had to distinguish between direct cell-cell transfer, which requires cell contact, ...
Genomic DNA transfer with a high-capacity adenovirus vector results in improved in vivo gene expression and decreased toxicity
Adenoviral vectors are frequently used for gene transfer because of their high cellular transduction efficiency in vitro and in ... Transfection / methods* * alpha 1-Antitrypsin / biosynthesis * alpha 1-Antitrypsin / genetics Substances * DNA-Binding Proteins ... Genomic DNA transfer with a high-capacity adenovirus vector results in improved in vivo gene expression and decreased toxicity ... Adenoviral vectors are frequently used for gene transfer because of their high cellular transduction efficiency in vitro and in ...
Gene transfer by the use of laser-induced stress wave: Cell type dependence of transfection efficiency<...
Gene transfer by the use of laser-induced stress wave : Cell type dependence of transfection efficiency. / Terakawa, Mitsuhiro ... Gene transfer by the use of laser-induced stress wave : Cell type dependence of transfection efficiency. European Conference on ... title = "Gene transfer by the use of laser-induced stress wave: Cell type dependence of transfection efficiency", ... Gene transfer by the use of laser-induced stress wave: Cell type dependence of transfection efficiency. ...
Robust Adenoviral and Adeno-Associated Viral Gene Transfer to the In Vivo Murine Heart | Circulation
PLB Transfection: Functional Studies. An important component of whole-heart transfection is the capacity to alter net chamber ... In Vivo Gene Transfer of PLB to the Myocardium of the PLB−/− Mouse. To test for chronic functionality of gene transfer, we ... For AdV transfection (using either LV or intravenous injection routes), hearts were stained 3 days after transfection, whereas ... 3 days after transfection), or AAVCMV β-gal (7 days after transfection). *P,0.05 vs control. **P,0.05 vs AdVCMV β-gal. B, ...
ReagentCellsReverse transfectionReagentsTransductionPlasmidVectorsOptimizationFluorescence resonancCalcium phosphate transfectionTransformationLiposomeEfficienciesLipidHigher transfectionStable transfectionEfficient transfectionSuccessful transfectionViralNonviralCytotoxicityVivoElectroporationCationic liposomesHigh-throughputPhysicochemical characteristicsMammalian cellRNAiMAXSiRNARecombinant ProteinsNeuronalNeuronsAdeno-assocImprovesSerumConventionalGene deliveryVectorMethodTherapeuticLipidsGenetic materialMSCsPolymerPlasmids
- Using LipofectAMINE, a widely used transfection reagent, we transfected 293T cells with a plasmid harboring the β-galactosidase (β-gal) gene. (springer.com)
- Felgner PL (1993) LipofectAMINE ™ reagent: a new, high efficiency polycationic liposome transfection reagent. (springer.com)
- These results suggest that FuGENE HD is the most preferred transfection reagent for many cell lines, followed by Arrest-In and jetPEI. (nyu.edu)
- and (ii) removing the non-complexed transfection reagent. (google.com)
- This is true for most of the reagent based transfection reactions. (marketsandmarkets.com)
- Fourth, the printed slide treated with transfection reagent is placed into a square dish with the printed side facing up. (wikipedia.org)
- Effectene Reagent is used in conjunction with the enhancer and the DNA condensation buffer (Buffer EC) to achieve high transfection efficiency. (wikipedia.org)
- The process of highly condensing DNA molecules and then coating them with Effectene Reagent is an effective way to transfer DNA into eukaryotic cells. (wikipedia.org)
- Chemical reagents were optimized for reagent-to-DNA ratio, transfection solution plating volume, and cell density. (cornell.edu)
- The following protocols provide instructions for lipid-mediated transfection of plasmid DNA or siRNA into Gibco Human Astrocytes using the Lipofectamine LTX Reagent or the Lipofectamine RNAiMax Reagent. (thermofisher.com)
- Lipofectamine LTX Reagent is a proprietary, animal-origin free formulation for the transfection of DNA into eukaryotic cells with low cytotoxicity. (thermofisher.com)
- Lipofectamine RNAiMAX Transfection Reagent (Cat. (thermofisher.com)
- Using PLUS™ Reagent enhances transfection performance in human astrocytes. (thermofisher.com)
- In this webinar, we discuss the key steps and protocol considerations for performing high-quality transfections on a consistent basis, taking into consideration variables such as cell type, culture conditions, reagent concentrations, and other critical parameters. (sciencemag.org)
- The time-consuming, error-prone DNA dispensing and multiplexing, but also the transfection reagent dispensing, are software-driven and performed by a nanodispenser device. (jove.com)
- It can dispense and multiplex DNA and transfection reagent according to a predesigned spreadsheet. (jove.com)
- The four-steps transfection protocol involves i) a diluent dispense with a classical liquid handler, ii) plasmid distribution and multiplexing, iii) a transfection reagent dispense by the nanodispenser, and iv) cell plating on the prefilled wells. (jove.com)
- Produce high titer lentivirus with the TransIT®-Lenti Transfection Reagent. (fishersci.com)
- The TransPass™ R2 Transfection Reagent is a two component non-liposomal reagent combination developed specifically for efficient transfection of siRNA in a variety of mammalian cell lines that include "difficult to transfect" cells such as primary cells, endothelial cells, lymphocytes, muscle cells, etc. (neb.com)
- HeLa cells were transfected with different concentrations of p38 MAPK ShortCut siRNA Mix (+) or 24 nM eGFP ShortCut siRNA Mix (C) using TransPass R2 Transfection Reagent. (neb.com)
- We show for the first time that up to 100,000 plasmid molecules can be delivered into individual cells, but also that DNA transfer into cells is a dynamic process that follows a defined kinetics of uptake and intracellular processing. (epfl.ch)
- Bead transfection of adherent cells. (mysciencework.com)
- Gene transfer into adherent mammalian cells using glass beads. (mysciencework.com)
- Bead transfection is a simple, rapid, efficient, and cost-effective method of gene transfer into adherent mammalian cells. (mysciencework.com)
- It involves a brief incubation of the cells with glass beads in a solution containing the DNA to be transferred. (mysciencework.com)
- Stable transfection efficiency assessed using the selectable marker gene neomycin phosphotransferase (NEOR) was 27% in COS-7 cells. (mysciencework.com)
- Thus, we asked whether Ras oncoproteins can transfer from melanoma to T cells, including tumor-infiltrating lymphocytes (TILs), and subsequently induce functional effects in the adopting T cells. (jimmunol.org)
- In this study, we show by an in vitro coculture system that GFP-tagged H-Ras, but not GFP, transfers from MEL526 to T cells and localizes to the inner aspect of their plasma membrane. (jimmunol.org)
- Notably, these various forms of tight contact formed between lymphocytes and target cells allow intercellular transfer of a large variety of molecules including membrane-associated proteins ( 5 , 6 ). (jimmunol.org)
- Even though the transfer of molecules among lymphocytes and the cells they scan is an intriguing topic, it is one of the less understood and studied aspects of cellular immunity. (jimmunol.org)
- Determination of cytotoxicity showed that the largest amount of viable cells was found after transfection with jetPEI and ExpressFect. (nyu.edu)
- After 48-hr culture, primed and transfected cells were assayed for transfection, cell proliferation, and cell viability by fluorescence measurement of EGFP reporter, Hoechst 33342 nuclei stain, and resazurin metabolic assay. (scienceexchange.com)
- The transferred material is ultimately processed and presented by DCs and can activate T cells. (rupress.org)
- It is based on the correction of pathologic gene expression patterns (e.g. by the transfer of tumor suppressor genes) or on the delivery of cytotoxic genes that directly or indirectly kill tumor cells irrespective of its gene expression. (aacrjournals.org)
- Consequently, poor efficiency of gene transfer potentially limits the number of vector-transduced tumor cells ( 6 ) and thus prevents effective systemic cancer gene therapy. (aacrjournals.org)
- Indeed, a small number of transduced cells that are accessible to the circulation could in theory mediate a much more pronounced effect that is relatively independent of gene transfer efficiency. (aacrjournals.org)
- For many disease models, the most desirable cell types such as immune system or primary cells are not amenable to transfection. (openwetware.org)
- The apparatus may be a catheter arrangement with various embodiments for applying heat to a patient's cells in vivo in order to improve transfection efficiency or application efficiency. (google.com)
- As a parallel development, due to maturation of technology and promising clinical data, the interest in redirecting adoptively transferred T cells by recombinant T cell receptors (TCRs) and chimeric antigen receptors (CARs) has moved into the spotlight [ 8 , 9 ], as has the pursuit of cancer-cell surface directed antibodies recruiting and activating immune effectors such as FcR positive immune cells (ADCC) or the complement cascade (CDC). (hindawi.com)
- These include the use of difficult-to-label target cells, or, regarding reporter gene transfection-based assays, the use of difficult-to-transfect targets such as primary human professional antigen presenting cells (APCs). (hindawi.com)
- However, there is no direct evidence of aggregate transfer between cells and the mechanism has not been explored. (biologists.org)
- By expressing recombinant fragments of mutant Htt in neuronal cells and in primary neurons, we found that aggregated fragments formed within one cell spontaneously transfer to neighbors in cell culture. (biologists.org)
- 1-3 Although cardiomyocytes are good vector targets, efforts have been impeded by low transfection efficiencies and difficulties in culturing and studying mature cells. (ahajournals.org)
- Transfection in hard to transfect cells is the need of hour and it is a major challenge. (marketsandmarkets.com)
- Transfection (the process of deliberately introducing genes into cells) technologies have been used in life science research and has been exploited for therapeutic delivery like for eg, gene therapy and electro-chemotherapy. (marketsandmarkets.com)
- Reverse transfection is a technique for the transfer of genetic material into cells. (wikipedia.org)
- As DNA is printed on a glass slide for the transfection process (the deliberate introduction of nucleic acids into cells) to occur before the addition of adherent cells, the order of addition of DNA and adherent cells is reverse that of conventional transfection. (wikipedia.org)
- The advantages of reverse transfection (over conventional transfection) are: The addition and attachment of target cells to the DNA-loaded surface can lead to a higher probability of cell-DNA contact, potentially leading to higher transfection efficiency. (wikipedia.org)
- Although equine MSCs have great importance, prior to this study, effective non-viral transfection parameters for these cells had not been determined. (cornell.edu)
- Transfection and Transformation of Human Thyroid Epithelial Cells. (barnesandnoble.com)
- We provide a theory for employing Förster resonance energy transfer (FRET) measurements to determine altered heteropentameric ion channel stoichiometries in intracellular compartments of living cells. (nih.gov)
- Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing means other than viral infection. (thermofisher.com)
- In transfection, the introduced nucleic acid may exist in the cells transiently, such that it is only expressed for a limited period of time and does not replicate, or it may be stable and integrate into the genome of the recipient, replicating when the host genome replicates. (thermofisher.com)
- Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. (thermofisher.com)
- Transformation is often used to describe non-viral DNA transfer in bacteria, non‑animal eukaryotic cells, and plant cells. (thermofisher.com)
- The two main purposes of transfection are to produce recombinant proteins, or to specifically enhance or inhibit gene expression in transfected cells. (thermofisher.com)
- Transfection is most commonly performed to express a protein of interest in cultured cells (or an animal model) through the use of a plasmid vector or mRNA. (thermofisher.com)
- Lipofectamine RNAiMAX is a proprietary formulation specifically developed for the transfection of siRNA and Stealth RNAi duplexes into eukaryotic cells. (thermofisher.com)
- make sure that cells are healthy and greater than 90% viable before transfection. (thermofisher.com)
- Transfection of cells in culture is a key technique in the molecular and cell biology toolbox. (sciencemag.org)
- Transfection refers to a DNA insertion into eukaryotic animal cells. (mito-hollyhock.net)
- The methods of introducing genes into cells can be performed in liquids (solution-based) or on the surface of a solid substrate (solid phase gene transfection). (nanowerk.com)
- In the solid phase gene transfection, DNA is fixed on the solid surface and then cells adhere on the DNA-bearing surface. (nanowerk.com)
- However, this approach had been considered problematic in clinical application situations, where the gene transferred cells are returned to the patient s body. (nanowerk.com)
- This protocol describes high-throughput plasmid transfection of mammalian cells in a 384-well plate using acoustic droplet ejection technology. (jove.com)
- The method presented here describes in detail how to perform DNA plasmid multiplexing and transfection in mammalian cells at high throughput using an acoustic-based liquid nanodispenser in a 384-well plate, even for nonspecialists in the field. (jove.com)
- Differences can come from the transfection lipid chosen, the concentrations of the lipids and nucleic acids, and the number of cells plated. (beckman.com)
- Chemical based methods of gene delivery can use natural or synthetic compounds to form particles that facilitate the transfer of genes into cells. (wikipedia.org)
- Nanogels enable efficient miRNA delivery and target gene downregulation in transfection-resistant multiple myeloma cells. (bioportfolio.com)
- While the delivery of growth inhibiting miRNA to multiple myeloma cells would be a promising strategy to evaluate treatment options, most multiple myeloma cells are transfection-resistant with established methods. (bioportfolio.com)
- These TAT/miRNA loaded nanogels delivered and released miR-34a with high efficiency into OPM-2 multiple myeloma cells that are known as transfection-resistant. (bioportfolio.com)
- Miami Winter Symposia, Volume 16: From Gene to Protein: Information Transfer in Normal and Abnormal Cells presents the expression and processing of genetic information at the levels of both proteins and nucleic acids. (elsevier.com)
- 6. Gene transfer and a mplification in mammalian cells (F. Wurm). (booktopia.com.au)
- 7. Co-transfer of multiple p lasmids/viruses to introduce several genes in mammalian cells (F. Wurm). (booktopia.com.au)
- Efficient Gene Transfer into Mammalian Primary Endocrine Cells with Lipopolyamine-coated DNA, Proceedings of the National Academy of Sciences of USA , 86, 6982-6986 (1989) Month Unavailable. (patents.com)
- Cationic liposomes are effective non-viral transfection of animal cells in vitro (Felgner P., G. Ringold, Nature 337/1989 /, 387-388). (google.com)
- Transfection is the process of deliberately introducing DNA or RNA into cells . (wikipedia.org)
- Transfection can result in unexpected morphologies and abnormalities in target cells. (wikipedia.org)
- Transfection with RNA molecules produces changes which cannot be permanently transmitted down a line of cells. (wikipedia.org)
- Transfection of animal cells usually involves opening temporary pores (holes) in the cell membrane , to allow the cells to take up the vector. (wikipedia.org)
- It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. (cshlpress.com)
- Adoptive cell transfer (ACT) therapy focuses of using the patient's own cells for therapeutic treatment. (nexcelom.com)
- Here we present a method for the transfection of Cas9 RNP's into HEK293 FT cells using Thermo Fisher Lipofectamine ® RNAiMAX. (neb.com)
- Seed the cells so that they will be around 70-90% confluent on the day of transfection. (neb.com)
- These cells are then transferred to recipient embryos to populate the gonads. (scielo.br)
- Optimization and characterization of calcium phosphate transfection in mesenchymal stem cells. (stanford.edu)
- In this report, we optimized the protocol of calcium phosphate transfection for murine MSCs, and compared this protocol with other gene transfer methods in different strains of mice and in human cells. (stanford.edu)
- Values of zeta potential of the liposomes were well consistent with those of transfection efficiency of pSV2CAT into the cells. (nii.ac.jp)
- This suggested that a novel cationic cholesterol derivative should be very promising in liposome-mediated gene transfection of plasmid and antisense DNA into target cells. (nii.ac.jp)
- From the results it was found that the vesicles with moderate diameters (from 0.4 to 1.4 mum) were most effective for gene transfection of plasmid DNA into the target cells. (nii.ac.jp)
- The most common way to study gene function is to transfer the gene into cells. (medindia.net)
- Currently, non-viral gene delivery methods demand a trade-off between efficiency of gene transfer and survival of the target cells, making these methods ineffective for genetic-modification of some important cell types. (medindia.net)
- The transfection efficiency of U-87 (human glioblastoma) cells was evaluated using fluorescence microscopy and flow cytometry. (dovepress.com)
- The introduction and subsequent expression of foreign DNA inside living mammalian cells (transfection) is achieved by photoporation with a violet diode laser. (osapublishing.org)
- These constructs are referred to as "infectious clones", as transfection of the BAC construct into host cells is sufficient to initiate viral infection. (wikipedia.org)
- Schematic representation of the optimal automated high-throughput reverse transfection protocol (from experimental design to custom biological assay). (jove.com)
- This is a 'reverse transfection' method that uses a final concentration of 10 nM RNP per transfection in a 96-well culture plate. (neb.com)
- Home brew reagents are still the preferred choice of reagents for transfection by researchers all over the world. (marketsandmarkets.com)
- She has led product development for Novagen and EMD Biosciences (now EMD Millipore) in the areas of transfection reagents and expression vectors. (sciencemag.org)
- Nucleic acid transfection is a procedure used in nearly any cellular laboratory and the abundance of commercially available reagents has made this a seemingly simple endeavor. (beckman.com)
- Successful gene transfer into murine pancreatic islets using polyamine transfection reagents. (nii.ac.jp)
- 이번 실험에서는 외부 gene을 mammalian cell 내로 옮겨 발현시키는 여러 가지 방법 중 transfection과 transduction에 대해 알아 보았다. (kyobobook.co.kr)
- Adenoviral vectors are frequently used for gene transfer because of their high cellular transduction efficiency in vitro and in vivo. (nih.gov)
- Transduction is used to describe virus-mediated DNA transfer. (thermofisher.com)
- In the NOD model, rIL-10 protein, IL-10 fusion protein, expression plasmid, or adoptive transfer with IL-10-cDNA-transduced islet-specific T cell clones have all been shown to prevent the onset of spontaneous diabetes ( 6 , 7 , 8 , 9 ). (jimmunol.org)
- To evaluate the in vitro and in vivo tolerance and transfection efficiency in ocular surface structures of a new nanoparticle system loaded with a plasmid coding for a modified MUC5AC protein (pMUC5AC). (arvojournals.org)
- Here we present an optimal protocol to perform ADE-based high-throughput plasmid transfection which makes it possible to reach an efficiency of up to 90% and a nearly 100% cotransfection in cotransfection experiments. (jove.com)
- The described software-based control of ADE plasmid multiplexing and transfection allows even nonspecialists in the field to perform a reliable cell transfection in a fast and safe way. (jove.com)
- This recently published method 1 allows performing as much as 384 independent plasmid DNA multiplexing and transfection conditions in one experiment, in less than 1 h. (jove.com)
- However, whether one is transfecting plasmid DNA or inhibitory RNAs, different cell lines can have significant differences in the ease of transfection or cellular survival. (beckman.com)
- Transfection efficiencies of several polymeric gene carriers were compared and correlated quantitatively to the amounts of cellular accumulation of plasmid DNA and to the expression of mRNA by quantitative real time PCR. (mit.edu)
- The results showed that the diameter of the complex was well related to the transfection activity of plasmid DNA to cultured cell lines. (nii.ac.jp)
- Nonviral gene delivery methods are advantageous over viral vectors in terms of safety, cost, and flexibility in design and application, but suffer from lower gene transfer efficiency. (scienceexchange.com)
- Viral delivery of RNAi vectors is a powerful alternative to transfection for these cell types as well as for in vivo applications. (openwetware.org)
- These results indicate significant advantages of regulated gene expression using genomic DNA for gene transfer and of adenoviral gene transfer vectors devoid of all viral coding sequences. (nih.gov)
- The use of viral-based vectors for myocardial gene transfer is an attractive approach for studying fundamental mechanisms of cardiac function and developing novel disease treatments. (ahajournals.org)
- Besides gelatin, atelocollagen and fibronetin are also successful transfection vectors for introducing foreign DNA into the cell nucleus. (wikipedia.org)
- These synthetic vectors have the ability to electrostatically bind DNA or RNA and compact the genetic information to accommodate larger genetic transfers. (wikipedia.org)
- Other techniques used successfully in poultry include cell transfection with retroviral vectors, lipofection in ovo , electroporation and biolistics (Muramatsu et al . (scielo.br)
- Recently the study of the interaction of GPCRs with their specific signaling and regulatory proteins has been widely studied using energy transfer-based approaches BRET and FRET (bioluminescence/fluorescence resonance energy transfer) ( 1 - 5 ). (frontiersin.org)
- Fluorescence resonance energy transfer (FRET) and co-immunoprecipitation techniques were employed to determine the effects of functional variants. (biomedcentral.com)
- Methods and Results- With the use of fluorescence resonance energy transfer, coimmunoprecipitation, and subcellular colocalization methods, it was found that TRPC1 interacts physically with TRPV4 to form a complex. (ahajournals.org)
Calcium phosphate transfection1
- We found that transfection efficiency and cell viability showed an inverse relationship depending on serum concentration during the process of calcium phosphate transfection, in which 2% serum was chosen in the optimized protocol. (stanford.edu)
- Although transformation in this sense can arise from infection with a transforming virus or from gene transfection, it can also arise spontaneously or following external stressors such as ionizing radiation or chemical mutagens. (thermofisher.com)
- When genes are delivered to bacteria or plants the process is called transformation and when it is used to deliver genes to animals it is called transfection. (wikipedia.org)
- They are transferred over to these genetic disease models by electroporation/transformation, transfection with a suitable virus or microinjection. (wikipedia.org)
- The invention also pertains to new methods of direct liposomal gene transfer in vivo, characterized in that liposome-DNA complexes can be applied repeatedly using automatic or reprimable pump systems either continuously or with purposeful time intervals. (google.com)
- Publications] R.Okayama: 'Cationic cholesterol with a hydroxyethylamino head group promotes significantly liposome-mediated gene transfection. (nii.ac.jp)
- High-throughput screening of clinically approved drugs that prime polyethylenimine transfection reveals modulation of mitochondria dysfunction response improves gene transfer efficiencies. (scienceexchange.com)
- This technique resulted in transfection of 54% of the cell population, a percentage that is on the higher end of nonviral MSC transfection efficiencies. (cornell.edu)
- A high efficiency transfection protocol employing a common polycationic lipid is described. (springer.com)
- Thus, a dramatic increase in transfection efficiency can be obtained by simply repeating transfection with the use of a common polycationic lipid. (springer.com)
- In this research, we investigate if lipid packaging can give further insights into transfection efficacy. (mdpi.com)
- PITPs can transfer phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes, exchanging PI for PC and vice versa, in order to maintain balanced membrane lipid levels upon consumption of phosphoinositides ( Wiedemann and Cockcroft, 1998 ). (elifesciences.org)
- The physicochemical characteristics, cytotoxicity, and transfection efficiency of cationic vesicles prepared from various molar ratios of amphiphilic surfactant Tween 80 (T), squalene (S), cationic charge lipid didodecyldimethylammonium bromide, and polyethylenimine were investigated. (dovepress.com)
- Of the lipoplexes prepared, those formulated with DSTAP showed higher transfection efficacy, however, the effect of buffer on transfection efficiency was formulation dependent. (mdpi.com)
- While transient transfection is advantageous for fast analysis of shRNA mediated effects, stable transfection ensures long-term, reproducible as well as defined shRNA effects. (openwetware.org)
- Stable transfection, on the other hand, provides the means for the bioproduction of various therapeutic molecules. (thermofisher.com)
- These systems allow stable transfection of recombinant constructs, and can permit inducible expression of precursor molecules. (thermofisher.com)
- We report here for the first time efficient transfection of mdx mice myofibers with dystrophin gene constructs delivered in vivo by the microsphere particles MF-2. (nature.com)
- Further efforts have been made to develop dedicated tools to enhance the ease of use while avoiding human errors during the overall process and to promote successful transfection even for nonspecialists in the field. (jove.com)
- As this technique delivers high transfection efficiency with little manipulation of the exogenous DNA and does not require the use of any viral sequences, it may be a useful alternative method of gene delivery in the development of gene therapy protocols. (mysciencework.com)
- In this study, we transferred the BCRF-1 gene, an open reading frame in the Epstein-Barr viral genome with remarkable homology to mouse IL-10 (viral IL-10 or vIL-10), by an adeno-associated viral (AAV) vector to NOD mice to attain sustained vIL-10 gene expression. (jimmunol.org)
- Background- Viral gene transfer to the whole heart in vivo has been achieved in several mammalian species but remained difficult to accomplish in murine hearts. (ahajournals.org)
- Conclusions- The new simplified approach for murine whole-heart viral transfection should assist molecular physiology studies. (ahajournals.org)
- Viral based transfection. (marketsandmarkets.com)
- The major types of Transfection include physical based (such as electroporation, nucleofection, magnetofection and gene gun), biochemical based(lipofection, calcium phosphate and dextran mediated) and viral based(adeno viruses and adeno-associated viruses based) transfection. (marketsandmarkets.com)
- Classically, the term transfection was used to denote the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, resulting in an infection and the production of mature virus particles. (thermofisher.com)
- However, the term transfection is also used to refer to infecting a cell specifically with viral nucleic acid that is isolated either from a eukaryote virus or from a bacteriophage. (thermofisher.com)
- His laboratory focuses on improving viral vector design, delivery efficiency, and vector safety for gene transfer applications. (sciencemag.org)
- Non-viral nanoparticulate transfection systems are particularly promising in this context, but so far struggle with transfection and knockdown efficiency. (bioportfolio.com)
- In addition to modifications to nucleic acid design and nonviral carriers, new tools are sought to enhance transfection. (scienceexchange.com)
- With the emerging fields of cancer vaccines and adoptive cell transfer therapies, there is an increasing demand for high-throughput in vitro cytotoxicity assays that efficiently analyze immune effector functions. (hindawi.com)
- Determining the optimal plating conditions for high transfection efficiency and low cytotoxicity can be highly involved. (beckman.com)
- 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium, inner salt assay, fluorescence microscope images, and flow cytometry analyses confirmed that (T:S) 1,040 µM with 4.3 µg/mL of PEI vesicles provided effective transfection without significant cytotoxicity. (dovepress.com)
- Finally, this heterotypic bystander effect is accompanied by a suppression of tumor growth in vivo that is independent of primary gene transfer into host-derived tumor vascular endothelium. (aacrjournals.org)
- The present study tested this methodology for both adenovirus (AdV)-mediated and adeno-associated virus (AAV)-mediated gene transfer to the in vivo murine heart. (ahajournals.org)
- In vivo tolerance and transfection efficiency was studied in C57BL/6 mice. (arvojournals.org)
- We tested the involvement of nuclear factor κB (NFκB) in the sodium channel dysfunction and evaluated the effects of in vivo transfection of manganese superoxide dismutase gene and NFκB shRNA on the baroreflex function in CHF rats. (ahajournals.org)
- In vivo transfection of adenoviral manganese superoxide dismutase gene or lentiviral NFκB p65 shRNA into the nodose ganglia partially reversed CHF-reduced sodium channel expression and cell excitability in the baroreceptor neurons and improved CHF-blunted arterial baroreflex sensitivity. (ahajournals.org)
- There are few upcoming transfection technologies like nucleofection and magnetofection which are an off-shoot of electroporation, which have the potential to address these challenges but are currently cost prohibitive due to which the technologies may have a much slower uptake in the developing world. (marketsandmarkets.com)
- In addition, a particular method and apparatus are disclosed in which electroporation and/or mass transfer across a cell membrane are accomplished by securing a cell across an opening in a barrier between two chambers such that the cell closes the opening. (google.es)
- Whilst there is a large body of evidence looking at the design of cationic liposomes as transfection agents, correlates of formulation to function remain elusive. (mdpi.com)
- We have studied the use of the cationic liposomes for gene transfection. (nii.ac.jp)
- The results showed that zeta potential of cationic liposomes was one of the important factors which control gene transfection. (nii.ac.jp)
- The transfection efficiency by the new cholesterol derivative was much higher than those by the cationic liposomes containing cationic derivatives with a dialkylamino head group described above. (nii.ac.jp)
- Further, atomic force microscopy (AFM) was used for studying gene transfection mediated by cationic liposomes which contain a cationic cholesterol derivative. (nii.ac.jp)
- Publications] C.Kawaura: 'Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative. (nii.ac.jp)
- Publications] M.Nakanishi: 'Intracellular localization of antisense DNA transferred by cationic liposomes with a cationic cholesterol derivative. (nii.ac.jp)
- To develop a library of transfection priming compounds, a high-throughput screen was performed of the NIH Clinical Collection (NCC) to identify clinical compounds that prime polyethylenimine (PEI) transfection. (scienceexchange.com)
- Each of the formulations was prepared by hydration in dH 2 O or phosphate buffer saline (PBS) to investigate the effect of buffer salts on lipoplex physicochemical characteristics and in vitro transfection. (mdpi.com)
- 1) Transfection lipids (Lipofectamine RNAiMAX (L), DharmaFECT 1-4, and negative control (0)), Opti-MEM media (light orange), and two siGLO Green concentrations (purple) were added to a 96-well plate. (beckman.com)
- The Span-8 pipettors and the selective tip feature of the multichannel head were used to serially dilute different lipids and combine them with two concentrations of a FAM-labelled siRNA (siGLO Green) which acted as a transfection readout. (beckman.com)
- Effective silencing of p38 MAPK is achieved at low siRNA nanomolar concentration 48 hours post-transfection. (neb.com)
- Transfection is an enabler technology used for many cell based research activities with applications spanning production of recombinant proteins and recombinant cell lines, gene therapy, delivery of therapeutics and also drug discovery. (marketsandmarkets.com)
- Neuronal transfection in brain slices using particle-mediated gene transfer. (nih.gov)
- Difficulties in neuronal transfection continue to restrict the applicability of molecular approaches to neurobiology. (nih.gov)
- In-tube transfection improves the efficiency of gene transfer in primary neuronal cultures. (rochester.edu)
- We describe here an alternative strategy using particle-mediated gene transfer for the transfection of neurons and glia in intact brain slices. (nih.gov)
- Additionally, transfection of adenoviral manganese superoxide dismutase also inhibited the augmentation of phosphorylated NFκB p65 in the nodose neurons from CHF rats. (ahajournals.org)
- This approach yielded robust cardiac transfection with adenovirus (AdV) and adeno-associated virus (AAV) injected into the left ventricle cavity or more simply via a central vein. (ahajournals.org)
- Under the direction of Dr. Deepak Agrawal , Moffitt researchers have developed a proprietary technology that dramatically improves the functionality and performance of transfection technology. (medindia.net)
- Transfections can be performed both in the presence or absence of serum. (thermofisher.com)
- Conventional transfection techniques have been of limited effectiveness, particularly in intact neural tissues. (nih.gov)
- 11 Here we describe the results of our recent findings in mdx mice (a biological model of DMD) after intramuscular gene delivery by means of synthetic microspheres (a novel type of transfer vehicle). (nature.com)
- The shRNA transfer vector alone can be transiently introduced into the dividing cell where the shRNA is synthesized by cellular machinery. (openwetware.org)
- Stable expression can be influenced by two factors: The transfection method used and the vector containing the shRNA of interest. (openwetware.org)
- Although there is still some debate as to the effectiveness of this approach, a regular shRNA transfer vector may be able to integrate into the genome of the target cell by antibiotic selection alone. (openwetware.org)
- Therefore, we examined the effects of transferring the cyclin-dependent kinase inhibitor p21 sdi1 gene into human esophageal cancer cell lines as well as normal keratinocytes using an adenovirus vector system. (aacrjournals.org)
- 따라서 DNA를 cell 속으로, 더구나 핵 속으로 까지 유입 시키는 것은 여러 가지 특별한 agent를 통한 transfection method를 사용해야 하는 까다로운 일이며, 높은 tranfection efficiency를 얻는 것이 transfection의 관건이다. (kyobobook.co.kr)
- The transfection method determines which cell type can be targeted for stable integration through antibiotic selection . (openwetware.org)
- As such, transfection is a powerful analytical tool for the study of the function and regulation of genes or gene products, for the production of transgenic organisms, and as a method for gene therapy. (thermofisher.com)
- This solid-mediated transfection has attracted attention due to the higher delivery efficiency of DNA than liquid phase transfection method. (nanowerk.com)
- We developed an easy, fast, and accurate method to perform cell transfection in a 384-well plate layout using acoustic droplet ejection (ADE) technology. (jove.com)
- there is a growing demand for new transfection technologies to address unmet needs for therapeutic delivery which is driving the transfection market. (marketsandmarkets.com)
- 1 Some excipients similar to cholesterol or squalene can be applied for stabilizing the vesicular structure and others such as cationic lipids or polymers can be used to achieve appropriate properties for the nucleic acid transfection. (dovepress.com)
- This is a general term for a vehicle used to transfer foreign genetic material into another cell. (wikipedia.org)
- Here both chemical and physical transfection methods were optimized for equine MSCs, using red fluorescent protein as a reporter gene. (cornell.edu)
- The transfection parameters determined in this study will undoubtedly be useful to all researchers who wish to genetically modify equine MSCs. (cornell.edu)
- To fulfill the requirements for different MSC therapies, safe and effective gene transfer methods for MSCs are critical. (stanford.edu)
- Results showed that the methods of polymer conjugation and the amount of VTW peptide were important factors to polymer synthesis and transfection. (dovepress.com)
- However, with the invention of different types of microarray printing systems, hundreds of transfection mixes (containing different DNA of interest) may be printed on the same slide for cell uptake of plasmids. (wikipedia.org)
- The described protocol includes a 'user-friendly' macro spreadsheet that we developed in order to manage 384 independent transfection conditions with multiplexing possibilities of up to four plasmids in each well. (jove.com)