Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.
Organic polymeric materials which can be broken down by naturally occurring processes. This includes plastics created from bio-based or petrochemical-based materials.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.
A group of thermoplastic or thermosetting polymers containing polyisocyanate. They are used as ELASTOMERS, as coatings, as fibers and as foams.
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.
Submicron-sized fibers with diameters typically between 50 and 500 nanometers. The very small dimension of these fibers can generate a high surface area to volume ratio, which makes them potential candidates for various biomedical and other applications.
A biocompatible polymer used as a surgical suture material.
Implants constructed of materials designed to be absorbed by the body without producing an immune response. They are usually composed of plastics and are frequently used in orthopedics and orthodontics.
Salts and esters of the 10-carbon monocarboxylic acid-decanoic acid.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone.
Procedures for enhancing and directing tissue repair and renewal processes, such as BONE REGENERATION; NERVE REGENERATION; etc. They involve surgically implanting growth conducive tracks or conduits (TISSUE SCAFFOLDING) at the damaged site to stimulate and control the location of cell repopulation. The tracks or conduits are made from synthetic and/or natural materials and may include support cells and induction factors for CELL GROWTH PROCESSES; or CELL MIGRATION.
A continuous protein fiber consisting primarily of FIBROINS. It is synthesized by a variety of INSECTS and ARACHNIDS.
The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.
Materials fabricated by BIOMIMETICS techniques, i.e., based on natural processes found in biological systems.
Synthetic or natural materials for the replacement of bones or bone tissue. They include hard tissue replacement polymers, natural coral, hydroxyapatite, beta-tricalcium phosphate, and various other biomaterials. The bone substitutes as inert materials can be incorporated into surrounding tissue or gradually replaced by original tissue.
The field of medicine concerned with understanding the biochemical basis of health and disease and involved in developing diagnostic and therapeutic methods that utilize MOLECULAR BIOLOGY techniques.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
Protein of the annexin family exhibiting lipid interaction and steroid-inducibility.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.
The physiological renewal, repair, or replacement of tissue.
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
Stents that are covered with materials that are embedded with chemicals that are gradually released into the surrounding milieu.
Biocompatible materials placed into (endosseous) or onto (subperiosteal) the jawbone to support a crown, bridge, or artificial tooth, or to stabilize a diseased tooth.
Exclusive legal rights or privileges applied to inventions, plants, etc.
The use of focused short radio waves to produce local hyperthermia in an injured person or diseased body area.
Electropositive chemical elements characterized by ductility, malleability, luster, and conductance of heat and electricity. They can replace the hydrogen of an acid and form bases with hydroxyl radicals. (Grant & Hackh's Chemical Dictionary, 5th ed)
Lipid-laden macrophages originating from monocytes or from smooth muscle cells.
The invasion of living tissues of man and other mammals by dipterous larvae.
Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
The study of natural phenomena by observation, measurement, and experimentation.
The application of scientific knowledge to practical purposes in any field. It includes methods, techniques, and instrumentation.
Ionized gases, consisting of free electrons and ionized atoms or molecules which collectively behave differently than gas, solid, or liquid. Plasma gases are used in biomedical fields in surface modification; biological decontamination; dentistry (e.g., PLASMA ARC DENTAL CURING LIGHTS); and in other treatments (e.g., ARGON PLASMA COAGULATION).
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
The quality or state of being wettable or the degree to which something can be wet. This is also the ability of any solid surface to be wetted when in contact with a liquid whose surface tension is reduced so that the liquid spreads over the surface of the solid.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Elements which exhibit atomic emission due to natural or artificial nuclear transformation. These elements spontaneously undergo radioactive decay.
Large collections of small molecules (molecular weight about 600 or less), of similar or diverse nature which are used for high-throughput screening analysis of the gene function, protein interaction, cellular processing, biochemical pathways, or other chemical interactions.
Techniques using energy such as radio frequency, infrared light, laser light, visible light, or acoustic energy to transfer information without the use of wires, over both short and long distances.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Application of principles and practices of engineering science to biomedical research and health care.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.
A type VI intermediate filament protein expressed mostly in nerve cells where it is associated with the survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
In tissue culture, hairlike projections of neurons stimulated by growth factors and other molecules. These projections may go on to form a branched tree of dendrites or a single axon or they may be reabsorbed at a later stage of development. "Neurite" may refer to any filamentous or pointed outgrowth of an embryonal or tissue-culture neural cell.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.

Embryonic stem cells cultured in biodegradable scaffold repair infarcted myocardium in mice. (1/1771)

Our previous findings demonstrated that directly injecting embryonic stem cells (ESCs) into ischemic region of the heart improved cardiac function in animals with experimental myocardial infarction (MI). Tissue engineering with stem cells may provide tissue creation and repair. This study was designed to investigate the effectiveness of grafting of ESC-seeded biodegradable patch on infarcted heart. MI in mice was induced by ligation of the left coronary artery. Mouse ESCs were seeded on polyglycolic-acid (PGA) material patches. Three days after culture, an ESC-seeded patch was transplanted on the surface of ischemic and peri-ischemic myocardium. Eight weeks after MI operation and patch transplantation, hemodynamics and cardiac function were evaluated in four (sham-operated, MI, MI + cell-free patch, and MI + ESC-patch) groups of mice. The blood pressure and left ventricular function were significantly reduced in the MI animals. Compared with MI alone and MI + cell-free patch groups, the animals received MI + ESC-seeded patches significantly improved blood pressure and ventricular function. The survival rate of the MI mice grafted with MI + ESC-seeded patches was markedly higher than that in MI alone or MI + cell-free patch animals. GFP-positive tissue was detected in infarcted area with grafting of ESC-seeded patch, which suggests the survivors of ESCs and possible myocardial regeneration. Our data demonstrate that grafting of ESC-seeded bioabsorbable patch can repair infarcted myocardium and improve cardiac function in MI mice. This novel approach of combining stem cells and biodegradable materials may provide a therapeutic modality for repairing injured heart.  (+info)

Instrumented fusion of thoracolumbar fracture with type I mineralized collagen matrix combined with autogenous bone marrow as a bone graft substitute: a four-case report. (2/1771)

In order to avoid the morbidity from autogenous bone harvesting, bone graft substitutes are being used more frequently in spinal surgery. There is indirect radiological evidence that bone graft substitutes are efficacious in humans. The purpose of this four-case study was to visually, manually, and histologically assess the quality of a fusion mass produced by a collagen hydroxyapatite scaffold impregnated with autologous bone marrow aspirate for posterolateral fusion. Four patients sustained an acute thoracolumbar fracture and were treated by short posterior segment fusion using the AO fixateur interne. Autologous bone marrow (iliac crest) impregnated hydroxyapatite-collagen scaffold was laid on the decorticated posterior elements. Routine implant removal was performed after a mean of 15.3 months (12-20). During this second surgery, fusion mass was assessed visually and manually. A bone biopsy was sent for histological analysis of all four cases. Fusion was confirmed in all four patients intraoperatively and sagittal stress testing confirmed mechanical adequacy of the fusion mass. Three out of the four (cases 2-4) had their implants removed between 12 and 15 months after the index surgery. All their histological cuts showed evidence of newly formed bone and presence of active membranous and/or enchondral ossification foci. The last patient (case 1) underwent implant removal at 20 months and his histological cuts showed mature bone, but no active ossification foci. This four-case report suggests that the fusion mass produced by a mineralized collagen matrix graft soaked in aspirated bone marrow is histologically and mechanically adequate in a thoracolumbar fracture model. A larger patient series and/or randomized controlled studies are warranted to confirm these initial results.  (+info)

Designer self-assembling peptide nanofiber scaffolds for adult mouse neural stem cell 3-dimensional cultures. (3/1771)

Biomedical researchers have become increasingly aware of the limitations of conventional 2-dimensional tissue cell culture systems, including coated Petri dishes, multi-well plates and slides, to fully address many critical issues in cell biology, cancer biology and neurobiology, such as the 3-D microenvironment, 3-D gradient diffusion, 3-D cell migration and 3-D cell-cell contact interactions. In order to fully understand how cells behave in the 3-D body, it is important to develop a well-controlled 3-D cell culture system where every single ingredient is known. Here we report the development of a 3-D cell culture system using a designer peptide nanofiber scaffold with mouse adult neural stem cells. We attached several functional motifs, including cell adhesion, differentiation and bone marrow homing motifs, to a self-assembling peptide RADA16 (Ac-RADARADARADARADA-COHN2). These functionalized peptides undergo self-assembly into a nanofiber structure similar to Matrigel. During cell culture, the cells were fully embedded in the 3-D environment of the scaffold. Two of the peptide scaffolds containing bone marrow homing motifs significantly enhanced the neural cell survival without extra soluble growth and neurotrophic factors to the routine cell culture media. In these designer scaffolds, the cell populations with beta-Tubulin(+), GFAP(+) and Nestin(+) markers are similar to those found in cell populations cultured on Matrigel. The gene expression profiling array experiments showed selective gene expression, possibly involved in neural stem cell adhesion and differentiation. Because the synthetic peptides are intrinsically pure and a number of desired function cellular motifs are easy to incorporate, these designer peptide nanofiber scaffolds provide a promising controlled 3-D culture system for diverse tissue cells, and are useful as well for general molecular and cell biology.  (+info)

Prospects of micromass culture technology in tissue engineering. (4/1771)

Tissue engineering of bone and cartilage tissue for subsequent implantation is of growing interest in cranio- and maxillofacial surgery. Commonly it is performed by using cells coaxed with scaffolds. Recently, there is a controversy concerning the use of artificial scaffolds compared to the use of a natural matrix. Therefore, new approaches called micromass technology have been invented to overcome these problems by avoiding the need for scaffolds. Technically, cells are dissociated and the dispersed cells are then reaggregated into cellular spheres. The micromass technology approach enables investigators to follow tissue formation from single cell sources to organised spheres in a controlled environment. Thus, the inherent fundamentals of tissue engineering are better revealed. Additionally, as the newly formed tissue is devoid of an artificial material, it resembles more closely the in vivo situation. The purpose of this review is to provide an insight into the fundamentals and the technique of micromass cell culture used to study bone tissue engineering.  (+info)

Biological designer self-assembling peptide nanofiber scaffolds significantly enhance osteoblast proliferation, differentiation and 3-D migration. (5/1771)

A class of self-assembling peptide nanofiber scaffolds has been shown to be an excellent biological material for 3-dimension cell culture and stimulating cell migration into the scaffold, as well as for repairing tissue defects in animals. We report here the development of several peptide nanofiber scaffolds designed specifically for osteoblasts. We designed one of the pure self-assembling peptide scaffolds RADA16-I through direct coupling to short biologically active motifs. The motifs included osteogenic growth peptide ALK (ALKRQGRTLYGF) bone-cell secreted-signal peptide, osteopontin cell adhesion motif DGR (DGRGDSVAYG) and 2-unit RGD binding sequence PGR (PRGDSGYRGDS). We made the new peptide scaffolds by mixing the pure RAD16 and designer-peptide solutions, and we examined the molecular integration of the mixed nanofiber scaffolds using AFM. Compared to pure RAD16 scaffold, we found that these designer peptide scaffolds significantly promoted mouse pre-osteoblast MC3T3-E1 cell proliferation. Moreover, alkaline phosphatase (ALP) activity and osteocalcin secretion, which are early and late markers for osteoblastic differentiation, were also significantly increased. We demonstrated that the designer, self-assembling peptide scaffolds promoted the proliferation and osteogenic differentiation of MC3T3-E1. Under the identical culture medium condition, confocal images unequivocally demonstrated that the designer PRG peptide scaffold stimulated cell migration into the 3-D scaffold. Our results suggest that these designer peptide scaffolds may be very useful for promoting bone tissue regeneration.  (+info)

In vitro chondrogenesis of mesenchymal stem cells in recombinant silk-elastinlike hydrogels. (6/1771)

PURPOSE: In this study the chondrocytic differentiation and cartilage matrix accumulation of human mesenchymal stem cells (hMSCs) were investigated after encapsulation in a genetically engineered silk-elastinlike protein polymer SELP-47 K as an injectable matrix for delivery of cell-based therapeutics. MATERIALS AND METHODS: hMSCs were encapsulated in SELP-47 K and cultured for 4 weeks in chondrogenic medium with or without transforming growth factor-beta3 (TGF). Chondrogenic differentiation was evaluated by histological, RNA and biochemical analyses for the expression of cartilage extracellular matrix components. RESULTS: Histological and immunohistochemical staining revealed that the cells acquired a rounded morphology and were embedded in significant amounts of chondrogenic extracellular matrix. Reverse transcriptase (RT)-PCR showed an up-regulation in aggrecan, type II and type X collagen and SOX9 in presence of TGF-beta3. By day 28, constructs cultured in the presence of TGF-beta3 exhibited significant increase in sulfated glycosaminoglycan and total collagen content up to 65 and 300%, respectively. CONCLUSIONS: This study demonstrates that SELP-47 K hydrogel can be used as a scaffold for encapsulation and chondrogenesis of hMSCs. The ability to use recombinant techniques to precisely control SELP structure enables the investigation of injectable protein polymer scaffolds for soft-tissue engineering with varied physicochemical properties.  (+info)

Porous silk scaffolds can be used for tissue engineering annulus fibrosus. (7/1771)

There is no optimal treatment for symptomatic degenerative disc disease which affects millions of people worldwide. One novel approach would be to form a patch or tissue replacement to repair the annulus fibrosus (AF) through which the NP herniates. As the optimal scaffold for this has not been defined the purpose of this study was to determine if porous silk scaffolds would support AF cell attachment and extracellular matrix accumulation and whether chemically decorating the scaffold with RGD peptide, which has been shown to enhance attachment for other cell types, would further improve AF cell attachment and tissue formation. Annulus fibrosus cells were isolated from bovine caudal discs and seeded into porous silk scaffolds. The percent cell attachment was quantified and the cell morphology and distribution within the scaffold was evaluated using scanning electron microscopy. The cell-seeded scaffolds were grown for up to 8 weeks and evaluated for gene expression, histological appearance and matrix accumulation. AF cells attach to porous silk scaffolds, proliferate and synthesize and accumulate extracellular matrix as demonstrated biochemically and histologically. Coupling the silk scaffold with RGD-peptides did not enhance cell attachment nor tissue formation but did affect cell morphology. As well, the cells had higher levels of type II collagen and aggrecan gene expression when compared to cells grown on the non-modified scaffold, a feature more in keeping with cells of the inner annulus. Porous silk is an appropriate scaffold on which to grow AF cells. Coupling RGD peptide to the scaffold appears to influence AF cell phenotype suggesting that it may be possible to select an appropriate scaffold that favours inner annulus versus outer annulus differentiation which will be important for tissue engineering an intervertebral disc.  (+info)

An overview of tissue engineering approaches for management of spinal cord injuries. (8/1771)

Severe spinal cord injury (SCI) leads to devastating neurological deficits and disabilities, which necessitates spending a great deal of health budget for psychological and healthcare problems of these patients and their relatives. This justifies the cost of research into the new modalities for treatment of spinal cord injuries, even in developing countries. Apart from surgical management and nerve grafting, several other approaches have been adopted for management of this condition including pharmacologic and gene therapy, cell therapy, and use of different cell-free or cell-seeded bioscaffolds. In current paper, the recent developments for therapeutic delivery of stem and non-stem cells to the site of injury, and application of cell-free and cell-seeded natural and synthetic scaffolds have been reviewed.  (+info)

Electrospun tissue engineering scaffolds are attractive due to their distinctive advantages over other types of scaffolds. As both osteoinductivity and osteoconductivity play crucial roles in bone tissue engineering, scaffolds possessing both properties are desirable. In this investigation, novel bicomponent scaffolds were constructed via dual-source dual-power electrospinning (DSDPES). One scaffold component was emulsion electrospun poly(D ,L -lactic acid) (PDLLA) nanofibers containing recombinant human bone morphogenetic protein (rhBMP-2), and the other scaffold component was electrospun calcium phosphate (Ca-P) particle/poly(lactic-co-glycolic acid) (PLGA) nanocomposite fibers. The mass ratio of rhBMP-2/PDLLA fibers to Ca-P/PLGA fibers in bicomponent scaffolds could be controlled in the DSDPES process by adjusting the number of syringes used to supply solutions for electrospinning. Through process optimization, both types of fibers could be evenly distributed in bicomponent scaffolds. The ...
The objective of this article is to systematically present the emerging understanding that 3D porous scaffolds serve not only as structural templates for tissue fabrication but also provide complex signaling cues to cells and facilitate oxygen and therapeutic agent delivery. Strategies in the field of tissue engineering and regenerative medicine often rely on 3D scaffolds to mimic the natural extracellular matrix as structural templates that support cell adhesion, migration, differentiation and proliferation, and provide guidance for neo-tissue formation. In addition to providing a temporary support for tissue fabrication, 3D scaffolds have also been used to study cell signaling that best mimics physiological conditions, thereby expanding our understanding beyond 2D cell cultures. It is now understood that cell responses to 3D scaffolds are distinctively different from 2D surfaces. Recently, 3D scaffolds emerged as a vehicle for improved oxygen transport to seeded cells and also to deliver relevant
The field of tissue engineering has advanced and evolved to focus on biomimetic strategies to meet the rise in demands of tissue replacements for surgical reconstruction. One of the key strategies focuses on developing growth factor delivery systems, by incorporating growth factors into tissue scaffolds. While growth factors are crucial cell-inducing components, their limitations such as short half-lives and dose related adverse effects remain a challenge. To overcome these challenges, this thesis is focused on the development of a novel biomimetic tissue scaffold concept incorporating cell-mediated activation of growth factors for cartilage regeneration. The latent transforming growth factor-β1 (TGF-β1) was selected as a model latent protein due to its well established effects on cartilage as well as its ubiquity in many other tissue types. The thesis first focused on the development and characterisation of the tissue scaffold. A non-woven fibrous scaffold was fabricated by electrospinning, ...
Skeletal muscle plays an important role in the bodys physiology but there are still no effective treatments for volumetric muscle loss (VML) resulting from severe traumatic injury or tumor excision. Recent studies show that a tissue engineering strategy using a compound containing mesenchymal stem cells (MSCs) and decellularized extracellular matrix (ECM) scaffold generates significant regenerative effects on VML injury, but the underlying mechanisms are not fully understood. The characteristics of human umbilical cord MSCs, including multiplication capacity and multidifferentiation ability, were determined. We constructed a compound containing MSCs and decellularized ECM scaffold which was used for tissue regeneration in a VML model. We found that MSCs and decellularized ECM scaffold generated synergistic effects on promoting skeletal muscle tissue regeneration. Interestingly, both MSCs and decellularized ECM scaffold could promote macrophage polarization toward the M2 phenotype and suppress
This review detailed the most commonly used biomaterial scaffolds for engineering tissues from stem cells by covering the types of materials available and their unique properties. This information allows readers to determine which material best suits their specific application. As mentioned in earlier in this review, many of these materials have not been fully optimized for specific tissue engineering applications and further work will continue to optimize these formulations for translation to the clinic for targeted applications. For example, optimized scaffolds could enhance the survival and differentiation of neural stem cells being transplanted into the diseased or damaged nervous system, which could lead to improved function. The type of material and the cues that are incorporated in the scaffold play a large role in directing the fate of the stem cells seeded inside as detailed in this review. The ability to further functionalize the materials discussed in this review in terms of their ...
Natural biomaterials such as collagen show promise in tissue engineering applications due to their inherent bioactivity. The main limitation of collagen is its low mechanical strength and somewhat unpredictable and rapid degradation rate; however, combining collagen with another material, such as chitosan, can reinforce the scaffold mechanically and may improve the rate of degradation. Additionally, the high cost and the risk of prion transmission associated with mammal-derived collagen has prompted research into alternative sources such as marine-origin collagen. In this context, the overall goal of this study was to determine if the incorporation of chitosan into collagen scaffolds could improve the mechanical and biological properties of the scaffold. In addition the study assessed if collagen, derived from salmon skin (marine), can provide an alternative to collagen derived from bovine tendon (mammal) for tissue engineering applications. Scaffold architecture and mechanical properties were ...
To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not yet been fully explored. Here, we report the potential use of silicate-containing hydroxyapatite (SiHA) microparticles and microparticle aggregates in the predominant range from 2.23 to 12.40 μm in combination with polycaprolactone (PCL) as a hybrid scaffold with randomly oriented and well-aligned microfibers for regeneration of bone tissue. Chemical and mechanical properties of the developed 3D scaffolds were investigated with XRD, FTIR, EDX and tensile testing. Furthermore, the internal structure and surface morphology of the scaffolds were analyzed using synchrotron X-ray μCT and SEM. Upon culturing human mesenchymal stem cells (hMSC) on PCL-SiHA scaffolds, we found that both SiHA inclusion and microfiber orientation affected cell adhesion. The best hMSCs
Many strategies for tissue engineering of replacement structures, such as heart valves, depend in part on preparing a tissue scaffold from a natural tissue matrix. An essential step in tissue scaffold fabrication is decellularization of the matrix. The objective of any decellularization method is twofold: (1) the preservation of the physical and biochemical properties of the extracellular matrix (ECM), and (2) the removal of all cellular material. Decellularization is currently done by contacting xenographic tissue with a combination of chemical detergents and biological agents. These processes can alter ECM structure and composition, which can trigger host immune response and inflammation. Currently, there are no accepted quantitative standards against which to certify the viability of the decellularized material. However, future standards will certainly include characteristics like removal of nuclear material, biochemical composition, and mechanical strength. We are evaluating a novel ...
We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73 and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic and chondrogenic and lost pluripotency - as seen with the loss of markers OCT3/4 and TRA-1-81 - and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new
Cardiac patch, which is a suitable alternative to heart transplant, recovers the heart tissue and ensures its sound functioning. The integration of advanced features such as therapeutic control through drug release is another groundbreaking innovation which is expected to be introduced in the cardiac patches and marketed globally in the coming decade. Such improvisations and additional features to the existing cardiac patches is anticipated to fuel the growth of the global cardiac patch market significantly during the 2017-2025 period. However, the limitations of cardiac patches such as the formation of aneurysms and the obstruction of growth potential because of ingrown tissue and calcification leads to multiple replacements and can hamper the market growth.. The global cardiac patch market can be segmented based on end-users and region. In terms of end-users, the market can be divided into hospitals and specialty clinics. The hospitals segment is likely to hold a major share in the global ...
A common problem in the design of tissue engineered scaffolds using electrospun scaffolds is the poor cellular infiltration into the structure. To tackle this issue, three approaches to scaffold design using electrospinning were investigated: selective leaching of a water-soluble fiber phase (poly ethylene oxide (PEO) or gelatin), the use of micron-sized fibers as the scaffold, and a combination of micron-sized fibers with codeposition of a hyaluronic acid-derivative hydrogel, Heprasil. These designs were achieved by modifying a conventional electrospinning system with two charged capillaries and a rotating mandrel collector. Three types of scaffolds were fabricated: medical grade poly(ε-caprolactone)/collagen (mPCL/Col) cospun with PEO or gelatin, mPCL/Col meshes with micron-sized fibers, and mPCL/Col microfibers cosprayed with Heprasil. All three scaffold types supported attachment and proliferation of human fetal osteoblasts. However, selective leaching only marginally improved cellular ...
2002). The tortuosity of scaffolds fabricated using solvent casting is not controllable because it requires the contact of the particulates during the fabrication procedure, which is a random process facilitated by using high humidity. Similarly, poor interconnectivity of pores is reported for scaffolds produced using gas foaming techniques, where only 10%À30% of the scaffolds pores are connected (Hutmacher, 2001). Control of pore size distribution in scaffolds fabricated using freeze-drying is an interesting research topic. 2010. The acellular matrix (ACM) for bladder tissue engineering: a quantitative magnetic resonance imaging study. Magn. Reson. Med. 64 (2), 341À348. 22404. , 2014. One-pot synthesis of macromesoporous bioactive glasses/polylactic acid for bone tissue engineering. Mater. Sci. Eng. C Mater. Biol. Appl. 43, 367À374. 1016/j. 042. Epub 2014 Jul 19. , 2014. Designer functionalised selfassembling peptide nanofibre scaffolds for cartilage tissue engineering. Expert Rev. Mol. ...
Biologic scaffold materials composed of extracellular matrix (ECM) have been used in a variety of surgical and tissue engineering/regenerative medicine
Biologic scaffolds composed of naturally occurring extracellular matrix (ECM) can provide a microenvironmental niche that alters the default healing response toward a constructive and functional outcome. The present study showed similarities in the remodeling characteristics of xenogeneic ECM scaffolds when used as a surgical treatment for volumetric muscle loss in both a preclinical rodent model and five male patients. Porcine urinary bladder ECM scaffold implantation was associated with perivascular stem cell mobilization and accumulation within the site of injury, and de novo formation of skeletal muscle cells. The ECM-mediated constructive remodeling was associated with stimulus-responsive skeletal muscle in rodents and functional improvement in three of the five human patients.. ...
Computational approaches have great potential for aiding clinical product development by finding promising candidate designs prior to expensive testing and clinical trials. Here, an approach for designing multilevel bone tissue scaffolds that provide structural support during tissue regeneration is developed by considering mechanical and biological perspectives. Three key scaffold design properties are considered: (1) porosity, which influences potential tissue growth volume and nutrient transport, (2) surface area, which influences biodegradable scaffold dissolution rate and initial cell attachment, and (3) elastic modulus, which influences scaffold deformation under load and, therefore, tissue stimulation. Four scaffold topology types are generated by patterning beam or truss-based unit cells continuously or hierarchically and tuning the element diameter, unit cell length, and number of unit cells. Parametric comparisons suggest that structures with truss-based scaffolds have higher surface ...
TY - JOUR. T1 - Biomechanical Comparison of Glutaraldehyde-Crosslinked Gelatin Fibrinogen Electrospun Scaffolds to Porcine Coronary Arteries. AU - Tamimi, E.. AU - Ardila, D. C.. AU - Haskett, D. G.. AU - Doetschman, T.. AU - Slepian, M. J.. AU - Kellar, R. S.. AU - Vande Geest, J. P.. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Cardiovascular disease (CVD) is the leading cause of death for Americans. As coronary artery bypass graft surgery (CABG) remains a mainstay of therapy for CVD and native vein grafts are limited by issues of supply and lifespan, an effective readily available tissue-engineered vascular graft (TEVG) for use in CABG would provide drastic improvements in patient care. Biomechanical mismatch between vascular grafts and native vasculature has been shown to be the major cause of graft failure, and therefore, there is need for compliance-matched biocompatible TEVGs for clinical implantation. The current study investigates the biaxial mechanical characterization of acellular electrospun ...
at New York University are testing out a 3D bone tissue scaffold printer that could substantially reduce the time it takes bones in the body to regenerate.. Bone generated from the scaffolds could be used to shore up alveolar (jaw) bone to support dental implants, as well as to repair cleft palates, fill in missing pieces of skull and repair other large and small defects.. The printer, known as a robotic deposition, or Robocaster, converts 3D information from CT scans into custom 3D printed tissue scaffolds with an unprecedented level of precision.. Because the structural elements of the scaffolds are similar in size to ingrowing bone structure (around 200µm), the bone is expected to grow faster and more accurately than bone generated from other types of tissue scaffolds.. The scaffolds are made from composites of hydroxyapatite and tricalcium phosphate that remodel with bone. Unlike metal plates used for bone replacement, the scaffolds disappear completely from the body once the bone has ...
Cell supports based on electroactive materials, that generate electrical signal variations as a response to mechanical deformations and vice-versa, are gaining increasing attention for tissue engineering applications. In particular, poly(vinylidene fluoride), PVDF, has been proven to be suitable for these applications in the form of films and two-dimensional membranes. In this work, several strategies have been implemented in order to develop PVDF three-dimensional scaffolds. Three processing methods, including solvent casting with particulate leaching and three-dimensional nylon, and freeze extraction with poly(vinyl alcohol) templates are presented in order to obtain three-dimensional scaffolds with different architectures and interconnected porosity. Further, it is shown that the scaffolds are in the electroactive β-phase and show a crystallinity degree of ~ 45%. Finally, quasi-static mechanical measurements showed that an increase of the porous size within the scaffold leads to a tensile strengths
Optimized electrospinning conditions were applied to produce single and multilayered (ML) scaffolds composed of polycaprolactone, collagen and elastin. The ML scaffold was cross-linked with glutaraldehyde to increase the stability. Morphological and structural characteristics of the scaffolds were measured by SEM and FTIR analyses. Results revealed that polymers combined to each other well and uniform fibers were obtained with the diameters ranging from 156 +/- 53 to 1536 +/- 293 nm. Contact angle measurements were performed to investigate the hydrophilic character of each structure. It was observed that incorporation of the natural polymers into the blends increased the hydrophilicity. Mechanical tests proved that collagen contributed to fabricate stiffer structures while elastin provided more elasticity. Biocompatibility of the scaffolds was examined by SEM analysis and WST-1 test with mouse fibroblast cells (L929) in vitro. Results exhibited that the addition of natural polymers increased the ...
Fingerprint Dive into the research topics of Cell detachment from porous poly(L-Lactic Acid) scaffolds cultured under flow perfusion for bone tissue engineering. Together they form a unique fingerprint. ...
The engineering of dermal skin substitutes, using autologous fibroblasts, requires high seeding efficiencies, a homogeneous cell distribution in the scaffolds, and optimal culture conditions. Dynamic seeding in spinner flasks was used to seed and subsequently culture fibroblasts in three-dimensional scaffolds. Several seeding and culture variables were investigated. Simulation of medium movement with microspheres showed that three different regions existed in medium (outer, middle, and inner), where overall particle movement was different. In the middle region the flow was turbulent and scaffolds were best placed in this region. After fibroblast seeding, methylene blue staining and scanning electron microscopy analysis of the scaffolds showed that at a low stirring speed (20 rpm) fibroblasts attached mainly onto the upper part of the scaffold, and at 40 and 60 rpm fibroblasts attached and spread throughout the scaffolds. Measurements of total DNA content per scaffold showed that lower stirring ...
agp-version 2.0 # ORGANISM: Homo sapiens # TAX_ID: 9606 # ASSEMBLY NAME: EG1 # ASSEMBLY DATE: 09-November-2011 # GENOME CENTER: NCBI # DESCRIPTION: Example AGP specifying the assembly of chromosome Y from WGS scaffolds # COMMENTS: # Three scaffolds are placed but have unknown orientation. chrY 1 10000 1 N 10000 telomere no na chrY 10001 13043 2 W EG1_scaffold1 1 3043 ? chrY 13044 63043 3 N 50000 contig no na chrY 63044 3434094 4 W EG1_scaffold2 1 3371051 + chrY 3434095 3484094 5 N 50000 contig no na chrY 3484095 3576421 6 W EG1_scaffold3 1 92327 + chrY 3576422 3626421 7 N 50000 contig no na chrY 3626422 3633571 8 W EG1_scaffold4 1 7150 + chrY 3633572 3683571 9 N 50000 contig no na chrY 3683572 3689149 10 W EG1_scaffold5 1 5578 + chrY 3689150 3739149 11 N 50000 contig no na chrY 3739150 3817095 12 W EG1_scaffold6 1 77946 + chrY 3817096 3867095 13 N 50000 contig no na chrY 3867096 5466918 14 W EG1_scaffold7 1 1599823 + chrY 5466919 5516918 15 N 50000 contig no na chrY 5516919 6945193 16 W ...
Highly aligned nanofibers created by fibroblasts form a biological scaffold that could prove an ideal foundation for engineered tissues. Stem cells placed on
In previous in vitro and in vivo studies, decellularized adipose tissue (DAT) has demonstrated unique bioactivity, but little is known about the bioactive components preserved in the decellularized scaffold. With the goal of characterizing the bioactive components in the DAT, protein was extracted from DAT samples from 3 donors using 5 different buffers. The resulting DAT extracts were found to have very low protein content so molecular weight fractioning centrifugation was used to concentrate the samples. Concentrated extracts were screened for the presence of the bioactive components adiponectin, vascular endothelial growth factor A (VEGF-A), bone morphogenetic protein 2 (BMP-2) and Dickkopf related protein 1 (DKK-1) using Western blotting. Positive signal for BMP-2 was found for one donor in Rogers Sample Buffer and Urea Buffer, but all other proteins investigated with Western blotting went undetected for all extraction buffers. Immunohistochemistry (IHC) was also used to determine the ...
Dr. Manitha B. Nair, Dr. Deepthy Menon, and Shantikumar V. Nair, Porous Composite Fibrous Scaffold for Bone Tissue Regeneration, U.S. Patent 15/341,866 2016.. ...
The most important finding in the present case was that in addition to favourable clinical, functional and radiographic results, transformation of the cell-free COL1 scaffold took place. The biopsy showed no signs of remaining COL1, but instead showed COL2 with embedded vital chondrocytes.. Several studies have shown that the use of cell-free scaffolds leads to favourable results comparable to those for cell-seeded scaffolds in different animal models [10, 12]. Similar results regarding clinical and morphologic outcome after implantation of cell-free COL1 matrices in humans were recently published [13].. The clinical course for the present case is in line with these results. After implantation of the cell-free scaffold, clinical, functional and morphological assessment revealed continuing improvement over time. The deterioration in results at the latest follow-up can undoubtedly be attributed to the traumatic meniscal tear the patient suffered. This injury also explains the slight deterioration ...
Vascular Tissue engineering (VTE) has emerged as a promising approach to develop blood vessel substitutes. Investigators have explored the use of arterial tissue cells combined with various types of natural and synthetic scaffolds to make tubular constructs in order to develop a functional small-diameter arterial replacement graft. The grafts must mimic the unique viscoelastic nature of an artery and be non-disruptive to blood ?ow. Moreover, after implantation, the scaffold must be gradually populated by cells and replaced by extra cellular matrix; with this respect, it is crucial that this replacement takes place with a well-defined timescale. In this work tubular scaffolds for VTE were produced via Diffusion Induced Phase Separation. Several PLA/ PLLA blends (100/0, 90/10, 75/25 wt/wt) were utilized in order to tune the crystallinity of the scaffolds and thus the rate of biodegrada- tion. In-Vitro biological tests were carried out in order to estimate the non-cytotoxicity of the scaffolds and ...
TY - JOUR. T1 - Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity. AU - Takei, Takayuki. AU - Ijima, Hiroyuki. AU - Sakai, Shinji. AU - Ono, Tsutomu. AU - Kawakami, Koei. PY - 2005/11/20. Y1 - 2005/11/20. N2 - We fabricated a scaffold of poly (lactic acid) and acidic gelatin, a sustained-release carrier of basic fibroblast growth factor (bFGF), which is a positive regulator of angiogenesis. After 1 week of implantation into mesentery of rats, blood vessels induced in the bFGF-containing scaffolds were almost double those in the bFGF-free scaffolds. Further, histological examination of hepatocyte-immobilizing scaffolds retrieved after 1 week of implantation into the mesentery of the rats that received a 70% hepatectomy revealed that the bFGF-containing scaffolds were more efficient for immobilized cell survival than the bFGF-free scaffolds. As well, hepatocytes in the bFGF-containing scaffolds kept ...
Researchers have developed a stable collagen scaffold for use with glucose sensors. This will prevent loss of function when the ND3D porous collagen is integrated as a scaffold around implantable glucose sensors. This will help minimize tissue reactions while stimulating angiogenesis. This invention will be useful in the treatment of diabetes as well as with other implantable devices.
Tofu not only is a delicious vegetarian food, but also shows potential biomedical applications for its high protein content and typical porous scaffold structure. Herein, two kinds of porous soybean scaffolds were developed, the first based on the traditional tofu manufacturing processes, the second modified
Data Availability StatementThe datasets helping the conclusions of this article are included within the article. scanning electron microscopy (SEM). Moreover, scaffold properties, such as pore size and morphology of the cells, onto the scaffolds were evaluated using SEM. Furthermore, biocompatibility of these scaffolds was confirmed by 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results The matrix mineralization was proved by alizarin reddish staining, and the osteogenic media-treated cultures positively expressed osteocalcin and osteopontin markers. Moreover, qRT-PCR results confirmed the positive gene expression of osteopontin and osteonectin in the differentiated osteoblast-like cells. The results of behavior assessment of the cultured cells on electrospinning and freeze-dried scaffolds demonstrated the fact that behavior from the cultured cells in the freeze-dried PLGA/HA scaffolds was considerably much better than the electrospinning PLGA/HA scaffolds. Bottom ...
A medical device includes a polymer scaffold crimped to a catheter having an expansion balloon. A single piece sheath is placed over the scaffold immediately following crimping of the scaffold to the balloon. The single piece sheath is replaced by a two-piece sheath, which is removed prior to performing a medical procedure using the medical device.
A mammalian tissue scaffold and method for making a tissue scaffold including a rigid scaffold body of biocompatible glass fibers bonded together and in special alignment to define open channels within the scaffold to allow fluid flow into and within the scaffold.
SpinPlant produces a unique collagen scaffold. Our biological scaffold is the platform technology basis for bone and cartilage repair, wound healing and soft tissue regeneration. The unique characteristics of our biological scaffold create evident advantages towards competing products ...
Background and Objective: Tissue engineering is a new method for replacing damaged tissue components in order to improve its function. In this method, a porous scaffold mixed with polysaccharide and synthetic antioxidants is first produced and then stem cells are cultured inside it. In this study, the polycaprolactane-chitosan-tannic acid scaffold was used ...
The time span needed for obtaining a functional cartilage substitute using tissue engineering strategies, together with the need for specific patient oriented constructs has stimulated the growing interest for developing
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Establishing structure activity relationships (SARs) in hit identification during early stage drug discovery is important in accelerating hit confirmation and expansion. We describe the development of En Core, a systematic molecular scaffold enumeration protocol using single atom mutations, to enhance the application of objective scaffold definitions and to enrich SAR information from analysis of high-throughput screening output. A list of 43 literature medicinal chemistry compound series, each containing a minimum of 100 compounds, published in the Journal of Medicinal Chemistry was collated to validate the protocol. Analysis using the top representative Level 1 scaffolds this list of literature compound series demonstrated that EnCore could mimic the scaffold exploration conducted when establishing SAR. When EnCore was applied to analyze an HTS library containing over 200 000 compounds, we observed that over 70% of the molecular scaffolds matched extant scaffolds within the library after ...
Scaffolds are a core concept in medicinal chemistry and they can be the focus of multiple independent development efforts, over an extended period. Thus, scaffold associated properties can vary over time, possibly showing consistently increasing or decreasing trends. We posit that such trends characterize the attention that the community pays to a scaffold.. This application allows you to query ChEMBL for a scaffold (represented as a SMILES) and visualize properties of the compounds containing the scaffold over time. This functionality is analogous to Google Trends.. Currently the properties considered are. ...
Stem cells grew, multiplied and differentiated into brain cells on a new three-dimensional scaffold of tiny protein fragments designed to be more like a living body than any other cell culture system.
(Medical Xpress) -- Johns Hopkins scientists have discovered a scaffolding protein that holds together multiple elements in a complex system responsible for regulating pain, mental illnesses and other complex neurological ...
Repopulating acellular biological scaffolds with phenotypically best suited cells is normally a appealing approach for regenerating functional organs and tissue. as the model program. Decellularization and recellularization had been optimized and a well balanced isotope labeling technique originated to differentiate remnant protein constituting the initial scaffold from protein recently synthesized by reseeded cells. Turnover of matrix and mobile proteins and the consequences of cell-scaffold connections were elucidated. This system sheds brand-new light on tissues remodeling and the procedure of tissues regeneration and it is easily applicable to various other tissue and body organ systems. remodeling from the ECM. The complete workflow is normally summarized in Fig. 1. This research is the initial to investigate the dynamic romantic relationship between your matrix and its own resident cells offering biological system-wide understanding into the proteins turnover thats central to SU6668 ...
This multi-layered scaffold could be applicable for tissue engineering/regeneration, drug delivery and wound dressing. This process may be used for phase-wise delivery of the growth factors which would be suitable for the studies of developmental biology. The novelty disclosed is the various ratios of the therapeutics/pharmaceuticals like antimicrobial agents, analgesics, antioxidants, antiallergic, growth factors and/or other signaling molecules like Lysophosphatidic acid (LPA), either in free form or microparticles encapsulated or combination of both can be taken to formulate the multi-layered porous scaffold with different concentrations of natural or chemically modified collagen. Microparticles comprise minimum of two types of polymers e.g. gelatin, chitosan, alginate, casein, PLGA, PCL and multi-layer scaffold contains at least two layers out of which one may be thin film layer without therapeutics, may act as a protective layer, which is desirable for wound dressing. The multi-layered ...
Show moreCentral nervous system (CNS) injuries present one of the most challenging problems. Regeneration in the mammal CNS is often limited because the injured axons cannot regenerate beyond the lesion. Implantation of a scaffolding material is one of the possible approaches to this problem. Recent implantations by our collaborative research group using electrospun polyamide nanofibrillar scaffolds have shown promising results in vitro and in vivo. The physical properties of the tissue scaffolds have been neglected for many years, and it has only recently been recognized that significant aspects include nanophysical properties such as nanopatterning, surface roughness, local elasticity, surface polarity, surface charge, and growth factor presentation as well as the better-known biochemical cues.The properties of: surface polarity, surface roughness, local elasticity and local work of adhesion were investigated in this thesis. The physical and nanophysical properties of the cell culture ...
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We have successfully demonstrated a polarization-maintaining (PM) fused silica microfiber by adiabatically tapering a conventional PM fiber. Compared to standard single-mode microfibers, the proposed PM microfibers exhibit robust polarization, preserving characteristics under the presence of external perturbations, such as bending. A polarization-extinction ratio of ...
0001.scaffold00002 AUGUSTUS gene 1386 2772 0.12 + . ID=Bv_00001z1_qhas;Name=Bv_00001z1_qhas 0001.scaffold00002 AUGUSTUS mRNA 1386 2772 0.12 + . ID=Bv_00001z1_qhas.t1;Parent=Bv_00001z1_qhas;Name=Bv_00001z1_qhas.t1 0%;Note=cDNAcoverage_0% 0001.scaffold00002 AUGUSTUS five_prime_UTR 1386 1976 . + . ID=Bv_00001z1_qhas.t1.UTR;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS start_codon 1977 1979 . + 0 ID=Bv_00001z1_qhas.t1.start_codon;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS CDS 1977 2325 0.96 + 0 ID=Bv_00001z1_qhas.t1.CDS;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS intron 2326 2619 0.81 + . ID=Bv_00001z1_qhas.t1.intron;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS CDS 2620 2747 0.8 + 2 ID=Bv_00001z1_qhas.t1.CDS;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS stop_codon 2745 2747 . + 0 ID=Bv_00001z1_qhas.t1.stop_codon;Parent=Bv_00001z1_qhas.t1 0001.scaffold00002 AUGUSTUS three_prime_UTR 2748 2772 . + . ID=Bv_00001z1_qhas.t1.UTR;Parent=Bv_00001z1_qhas. ...
T cells are finicky. You cant just inject them into a tumour and have them go to work. They will die within a couple of days if they arent in an environment equipped with the right mix of nutrients and ways to eliminate waste. Plus, tumours release self-defense chemicals that stop T cells from working.. Stephan and his team have designed a homey environment to outwit those obstacles.. The sticky, spongey, dissolvable biopolymer scaffold consists of tiny pores and can be made into just about any size or shape. With collaborators at the Massachusetts Institute of Technology and University of Washington, Stephan and his Fred Hutch team loaded the scaffold with CAR-T cells and proteins to keep the T cells healthy, enable them to rapidly grow and quickly attack the tumour. The mix also included a substance known as STING agonist, which ramps up the immune response to go after nearby cancer cells that arent recognized by CAR-T cells.. When placed on the tumours of mice, the scaffolds sway the ...
The goal of the project is to quantitatively understand the role of one of the key regulatory cytokines, i.e. IL-4, in order to predict the outcome of the inflammatory process and to control the delicate balance between fibrotic or functional regenerated ECM production. The effect of the IL-4 boosted natural human host response on early tissue formation will be investigated in vitro. Data will be used to allow the development of an IL-4 loaded synthetic scaffold that selectively activates the wound healing M2 phenotype.
TY - JOUR. T1 - Effects of scaffold architecture on mechanical characteristics and osteoblast response to static and perfusion bioreactor cultures. AU - Bartnikowski, Michal. AU - Klein, Travis J.. AU - Melchels, Ferry P. W.. AU - Woodruff, Maria A.. PY - 2014/7. Y1 - 2014/7. N2 - Tissue engineering focuses on the repair and regeneration of tissues through the use of biodegradable scaffold systems that structurally support regions of injury while recruiting and/or stimulating cell populations to rebuild the target tissue. Within bone tissue engineering, the effects of scaffold architecture on cellular response have not been conclusively characterized in a controlled-density environment. We present a theoretical and practical assessment of the effects of polycaprolactone (PCL) scaffold architectural modifications on mechanical and flow characteristics as well as MC3T3-E1 preosteoblast cellular response in an in vitro static plate and custom-designed perfusion bioreactor model. Four scaffold ...
TY - JOUR. T1 - Simultaneous electrospin-electrosprayed biocomposite nanofibrous scaffolds for bone tissue regeneration. AU - Francis, Lijo. AU - Venugopal, J.. AU - Prabhakaran, Molamma P.. AU - Thavasi, V.. AU - Marsano, E.. AU - Ramakrishna, S.. PY - 2010/10. Y1 - 2010/10. N2 - Currently, the application of nanotechnology in bone tissue regeneration is a challenge for the fabrication of novel bioartificial bone grafts. These nanostructures are capable of mimicking natural extracellular matrix with effective mineralization for successful regeneration of damaged tissues. The simultaneous electrospraying of nanohydroxyapatite (HA) on electrospun polymeric nanofibrous scaffolds might be more promising for bone tissue regeneration. In the current study, nanofibrous scaffolds of gelatin (Gel), Gel/HA (4:1 blend), Gel/HA (2:1 blend) and Gel/HA (electrospin-electrospray) were fabricated for this purpose. The morphology, chemical and mechanical stability of nanofibres were evaluated by means of field ...
Surface properties of scaffolds such as hydrophilicity and the presence of functional groups on the surface of scaffolds play a key role in cell adhesion, proliferation and migration. Different modification methods for hydrophilicity improvement and introduction of functional groups on the surface of scaffolds have been carried out on synthetic biodegradable polymers, for tissue engineering applications. In this study, alkaline hydrolysis of poly (ε-caprolactone) (PCL) nanofibrous scaffolds was carried out for different time periods (1 h, 4 h and 12 h) to increase the hydrophilicity of the scaffolds. The formation of reactive groups resulting from alkaline hydrolysis provides opportunities for further surface functionalization of PCL nanofibrous scaffolds. Matrigel was attached covalently on the surface of an optimized 4 h hydrolyzed PCL nanofibrous scaffolds and additionally the fabrication of blended PCL/matrigel nanofibrous scaffolds was carried out. Chemical and mechanical characterization ...
3D bioprinting techniques have been attracting attention for tissue scaffold fabrication in nerve tissue engineering applications. However, due to the inherent complexity of nerve tissues, bioprinting scaffolds that can appropriately promote the regeneration of damaged tissues is still challenging. This paper presents our study on bioprinting Schwann cell-laden scaffolds from low-viscosity hydrogel compositions including RGD modified alginate, hyaluronic acid and fibrin, with a focus on investigating the printability of hydrogel compositions and characterizing the functions of printed scaffolds for potential use in nerve tissue regeneration. We assessed the rheological properties of hydrogel precursors via temperature, time and shear rate sweeps, and then designed/determined the bioprinting process parameters including printing pressure and needle type/size. Bioprinting with a submerged crosslinking method was applied for scaffold fabrication, where the key was to rigorously regulate the ...
TY - JOUR. T1 - Improvement of osteoblast functions by sustained release of bone morphogenetic protein-2 (BMP-2) from heparin-coated chitosan scaffold. AU - Yun, Young Pil. AU - Lee, Su Young. AU - Kim, Hak Jun. AU - Song, Jae-Jun. AU - Kim, Sung Eun. PY - 2013/1/1. Y1 - 2013/1/1. N2 - The aim of this study was to investigate the improvement in osteoblast functions by using bone morphogenetic protein-2 (BMP-2) immobilized heparin-coated chitosan scaffolds and comparing it with that using chitosan scaffold or BMP-2/chitosan scaffold in vitro. BMP-2 was released from the heparin-coated chitosan scaffold in a sustained manner compared to that released from the chitosan scaffold. The osteoblast functions of MG-63 cells grown on the chitosan scaffold, the BMP-2/chitosan scaffold, the BMP-2/Hep/chitosan scaffold were investigated by assessing cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of the in vitro studies demonstrated that MG-63 ...
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Background. The ADSCs, have the capacity for renewal and the potential to differentiate into multiple lineages of mesenchymal tissues. These cells are capable of forming bone when implanted in an appropriate scaffold. Aims. We evaluate the effect of reconstructing bone defect by using degradable porous scaffolds seeded with ADSCs and compare the suitability of different biomaterial porous scaffolds; Hydroxyapatite/beta-Tricalcium Phosphate, Beta-Tricalcium Phosphate, Calcium Meta phosphate , Collagen-coated CMP.. Methods. ADSCs were obtained 24 adult Beagles undergoing lipectomy. The cells were isolated, cultured, labeled and seeded in vitro. The experimental group received ADSCs scaffolds and the control group received the acellular scaffolds into femoral defects, respectively. The bone blocks were retrieved at 4, 8, 12 weeks after the surgery for radiographic, biochemistry, histomorphologic analysis.. Results. Histological analysis revealed that implants loaded with autologous ADSCs, had ...
Description: Nanostructured Bioactive Glasses for Hard Tissue Regeneration and Tissue Engineering. Amorphous bioactive materials as tissue engineering scaffolds can regenerate hard tissues using the natural repair mechanisms of the body. This presentation will highlight underlying investigations on nanostructured bioactive glasses in vitro and in vivo.. 3D scaffolds for craniofacial tissue engineering Large bony defects present special challenges in craniofacial surgeries. Two strategies in using 3D fabricated scaffolds to enhance bone regeneration in the craniofacial area will be discussed. Biologically Active Nanostructured Membranes for Periodontal Tissue Regeneration. Exogenous growth factors, bone grafting materials and cell-based approaches have contributed to the advancement of periodontal tissue regeneration. This presentation will address electrospinning-based strategies that are currently being used.. Injectable scaffolds for dental pulp tissue engineering. The development of scaffolds ...
Polycaprolactone (PCL) has been regarded as a promising synthetic material for bone tissue engineering application. Owing to its unique biochemical properties and great compatibility, PCL fibers have come to be explored as a potential delivering scaffold for stem cells to support bone regeneration during clinical application. The human derived mesenchymal stem cells (MSCs) were obtained from umbilical cord (UC), bone marrow (BM), and adipose tissue (AD), respectively. The osteogenic differentiation potency of various human MSCs on this novel synthetic biomaterial was also investigated in vitro. Here, we illustrated that those human UC-, BM-, and AD-derived MSCs exhibited fibroblast-like morphology and expressed characteristic markers. Impressively, PCL nanofiber scaffold could support those MSC adhesion and proliferation. Long-term culture on PCL nanofiber scaffold maintained the viability as well as accelerated the proliferation of those three different kinds of human MSCs. More importantly, the
Bone is the second most transplanted tissue in the body, with approximately 2.2 million bone graft procedures performed annually worldwide. Currently, autogenous bone is the gold standard for bone grafting due to its ability to achieve functional healing; however, it is limited in supply and results in secondary injury at the donor site. Tissue engineering has emerged as a promising means for the development of new bone graft substitutes in order to overcome the limitations of the current grafts. In this research project, the specific approach for bone tissue engineering involves seeding osteoprogenitor cells within a biomaterial scaffold then culturing this construct in a biodynamic bioreactor. The bioreactor imparts osteoinductive mechanical stimuli on the cells to stimulate the synthesis of an extracellular matrix rich in osteogenic and angiogenic factors that are envisioned to guide bone healing in vivo. Fluid flow, which exerts a hydrodynamic shear stress on adherent cells, has been ...
In recent years, significant success has been made in the field of regenerative medicine. Tissue engineering scaffolds have been developed to repair and replace different types of tissues. The overall goal of the current work was to develop scaffolds of native extracellular matrix components for soft tissue regeneration, more specifically, neural tissue engineering. To date, much research has been focused on developing a nerve guidance scaffold for its ability to fill and heal the gap between the damaged nerve ends. Such scaffolds are marked by several intrinsic properties including: (1) a biodegradable scaffold or conduit, consisting of native ECM components, with controlled internal microarchitecture; (2) support cells (such as Schwann cells) embedded in a soft support matrix; and (3) sustained release of bioactive factors. In the current dissertation, we have developed such scaffolds of native biomaterials including hyaluronic acid (HA) and collagen. HA is a nonsulphated, unbranched, ...
Background: Total meniscectomy leads to knee osteoarthritis in the long term. The poly(epsilon-caprolactone) (PCL) scaffold is a promising material for meniscal tissue regeneration, but cell-free scaffolds result in relatively poor tissue regeneration and lead to joint degeneration. Hypothesis: A novel, 3-dimensional (3D)-printed PCL scaffold augmented with mesenchymal stem cells (MSCs) would offer benefits in meniscal regeneration and cartilage protection. Study Design: Controlled laboratory study. Methods: PCL meniscal scaffolds were 3D printed and seeded with bone marrow-derived MSCs. Seventy-two New Zealand White rabbits were included and were divided into 4 groups: cell-seeded scaffold, cell-free scaffold, sham operation, and total meniscectomy alone. The regeneration of the implanted tissue and the degeneration of articular cartilage were assessed by gross and microscopic (histological and scanning electron microscope) analysis at 12 and 24 weeks postoperatively. The mechanical properties of
Surgical repairs of rotator cuff tears have high re-tear rates and many scaffolds have been developed to augment the repair. Understanding the interaction between patients cells and scaffolds is important for improving scaffold performance and tendon healing. In this in vitro study, we investigated the response of patient-derived tenocytes to eight different scaffolds. Tested scaffolds included X-Repair, Poly-Tape, LARS Ligament, BioFiber (synthetic scaffolds), BioFiber-CM (biosynthetic scaffold), GraftJacket, Permacol, and Conexa (biological scaffolds). Cell attachment, proliferation, gene expression, and morphology were assessed. After one day, more cells attached to synthetic scaffolds with dense, fine and aligned fibres (X-Repair and Poly-Tape). Despite low initial cell attachment, the human dermal scaffold (GraftJacket) promoted the greatest proliferation of cells over 13 days. Expression of collagen types I and III were upregulated in cells grown on non-cross-linked porcine dermis (Conexa).
Surgical repairs of rotator cuff tears have high re-tear rates and many scaffolds have been developed to augment the repair. Understanding the interaction between patients cells and scaffolds is important for improving scaffold performance and tendon healing. In this in vitro study, we investigated the response of patient-derived tenocytes to eight different scaffolds. Tested scaffolds included X-Repair, Poly-Tape, LARS Ligament, BioFiber (synthetic scaffolds), BioFiber-CM (biosynthetic scaffold), GraftJacket, Permacol, and Conexa (biological scaffolds). Cell attachment, proliferation, gene expression, and morphology were assessed. After one day, more cells attached to synthetic scaffolds with dense, fine and aligned fibres (X-Repair and Poly-Tape). Despite low initial cell attachment, the human dermal scaffold (GraftJacket) promoted the greatest proliferation of cells over 13 days. Expression of collagen types I and III were upregulated in cells grown on non-cross-linked porcine dermis (Conexa).
Surgical repairs of rotator cuff tears have high re-tear rates and many scaffolds have been developed to augment the repair. Understanding the interaction between patients cells and scaffolds is important for improving scaffold performance and tendon healing. In this in vitro study, we investigated the response of patient-derived tenocytes to eight different scaffolds. Tested scaffolds included X-Repair, Poly-Tape, LARS Ligament, BioFiber (synthetic scaffolds), BioFiber-CM (biosynthetic scaffold), GraftJacket, Permacol, and Conexa (biological scaffolds). Cell attachment, proliferation, gene expression, and morphology were assessed. After one day, more cells attached to synthetic scaffolds with dense, fine and aligned fibres (X-Repair and Poly-Tape). Despite low initial cell attachment, the human dermal scaffold (GraftJacket) promoted the greatest proliferation of cells over 13 days. Expression of collagen types I and III were upregulated in cells grown on non-cross-linked porcine dermis (Conexa).
There has been a considerable growth and development in electrospun nanofibers for research activity, as well as commercial fabrication over the past couple of decades. These continuous nanofibers are solution driven exclusively by an electric field. Numerous studies on electrospun fibrous scaffolds have demonstrated sufficient mechanical properties and support of cell growth for tissue engineering. Despite these substantial achievements, there is still an Edisonian-type procedure to acquire the desired scaffold orientation and mechanical response that mimics the native tissue behavior. In this study, the electrospun scaffolds are fabricated with different fiber orientation -i.e. aligned and patterned (0/90) - by modifying the electrospinning process, specifically electric field and target, over large areas and lengths (30 mm x 30 mm). Mechanical behavior of controlled scaffold parameters at nanoscale, e.g., fiber orientation, and porosity, is investigated for an effective tissue replacement. In
Introducing porosity in electrospun scaffolds is critical to improve cell penetration and nutrient diffusion for tissue engineering. Nanofibrous cellulose scaffolds were prepared by electrospinning cellulose acetate (CA) followed by saponification to regenerate cellulose. Using a computer-assisted design approach, scaffolds underwent laser ablation resulting in pores with diameters between 50 and 300 mu m. without damaging or modifying the surrounding scaffold area. A new mineralization method was employed in conjunction with microablation using commercial phosphate buffered saline (PBS) to soak carboxymethylcellulose surface-modified electrospun scaffolds. The resulting crystals within the scaffold on the interior of the pore had a calcium to phosphate ratio of 1.56, similar to hydroxyapatite. It was observed that porosity of the cellulose scaffolds enhanced osteoblast cell attachment at the edge of the pores, while mineralization enhanced overall cell density.
We have previously reported on the use of Bay K8644-release strategies in combination with perfusion-compression bioreactor systems for up regulating bone formation in three-dimensional PLLA scaffolds. Here we report on the analysis of Bay activity following its release from our PLLA scaffolds over the culture period imposed in our tissue engineering protocol using UV spectroscopy in combination with whole cell patch clamping techniques. Bay was released continually from scaffolds within the physiological range required for agonist activity (1-10 microM). Patch clamping allowed for the effects of Bay released from scaffolds to be monitored directly with respect to osteoblast electrophysiology. A characteristic shift in the current-voltage (I-V) relationship of L-type VOCC currents was observed in rat osteoblast sarcoma (ROS) cells patched in a solution with Bay released from scaffolds following 14 and 28 days incubation, with statistically significant differences observed in peak currents compared to
Polyurethane scaffolds (PUs) have a good biocompatibility but lack cell recognition sites. In this study, we functionalized the surface of a PU, P(D/L)LA and PCL (50:50) containing urethane segments, with heparin. The first step in this functionalization, aminolysis, lead to free amine groups on the surface of the PU. Free amine content was determined to be 6.4 nmol/mL/mg scaffold, a significant increase of 230%. Subsequently, heparin was crosslinked. Immunohistochemistry demonstrated the presence of heparin homogeneous throughout the 3D porous scaffold. Youngs modulus decreased significantly till 50% of the native stiffness after aminolysis and did not change after heparin crosslinking. Contact angle on PU films significantly decreased from 82.7 degrees to 64.3 degrees after heparin crosslinking, indicating a more hydrophilic surface. This functionalization beholds great potential for tissue engineering purposes. When used in a load-bearing environment, caution is necessary due to reduction in ...
TY - JOUR. T1 - Multivariate analysis of variance (MANOVA) on the microstructure gradient of biomimetic nanofiber scaffolds fabricated by cone electrospinning. AU - Wang, Min. AU - Zhou, Yingge. AU - Tan, George Z.. PY - 2019/8. Y1 - 2019/8. N2 - Biomimetic scaffolds for tissue engineering should exhibit structural complexity close to native tissues, which typically have non-homogenous nanostructures. This study presents a novel electrospinning process using a cone-shape rotating collector to fabricate polymer nanofiber scaffold with continuous gradient microstructures. The effects of rotation speed (RS) and tip-to-axis distance (TAD) on microstructure gradients were investigated through multivariate analysis of variance (MANOVA), a generalization of univariate ANOVA that allows the researchers to analyze more than one dependent variable in designed experiments. The covariation against multivariate null hypotheses relative to error covariation was visualized through an ellipsoid plot. We found ...
TY - JOUR. T1 - Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering. AU - Björninen, Miina. AU - Gilmore, Kerry. AU - Pelto, Jani. AU - Seppänen-Kaijansinkko, Riitta. AU - Kellomäki, Minna. AU - Miettinen, Susanna. AU - Wallace, Gordon. AU - Grijpma, Dirk. AU - Haimi, Suvi. N1 - EXT=Pelto, Jani. PY - 2016/11/14. Y1 - 2016/11/14. N2 - We investigated the use of polypyrrole (PPy)-coated polymer scaffolds and electrical stimulation (ES) to differentiate adipose stem cells (ASCs) towards smooth muscle cells (SMCs). Since tissue engineering lacks robust and reusable 3D ES devices we developed a device that can deliver ES in a reliable, repeatable, and cost-efficient way in a 3D environment. Long pulse (1 ms) or short pulse (0.25 ms) biphasic electric current at a frequency of 10 Hz was applied to ASCs to study the effects of ES on ASC viability and differentiation towards SMCs on the PPy-coated scaffolds. PPy-coated scaffolds promoted ...
Tissue engineering is a new concept emerged as an alternative approach to tissue and organ reconstruction. It differs from organ transplantation by regenerating patients own tissue and organs avoiding the biocompatibility and low biofunctionality problems as well as severe immune rejection; which are the main problems of organ transplantation. In tissue engineering approach developed in 3Bs Research Group, the scaffold performs a critical role. The architecture of the tissue engineered scaffold is an important factor to take into consideration that can modulate biological response and the clinical success of the scaffold. Despite the periodical and completely interconnected pore network that characterizes rapid prototyped (RP) scaffolds, cell seeding efficiency still remains a critical factor for optimal tissue engineering applications. Hierarchical fibrous scaffolds, obtained by the combination of RP micro- and electrospun nano-motifs, have been considered a solution to overcome this drawback. ...
Cardiac tissue engineering is an emerging field that may hold great promise for advancing the treatment of heart diseases. Cardiac tissue engineering is in its infancy, and the overall field of tissue engineering, which was formalized in the late 1980s at conferences and workshops sponsored by the National Science Foundation, is still new enough to warrant some description. By broad definition, tissue engineering involves the construction of tissue equivalents through the manipulation and combination of living cells and biomaterials. It is a multidisciplinary field combining diverse aspects of the life sciences, engineering, and clinical medicine. The overall goal of tissue engineering is to develop tissue equivalents for use in the repair, replacement, maintenance, or augmentation of tissues or organs. Although some aspects of cardiac tissue engineering research have been ongoing for generations, albeit without being known as such, directed efforts in the field are only beginning.. The main ...
Greengate is the medieval heart of the city of Salford and lies on the Salford - Manchester border. Greengate has been a key investment focus of the council for a number of years now, with a vision to create a new corporate centre for Salford.. There are still a number of key opportunities for further development within the area and Salford City Council therefore commissioned Urban Vision (planning consultants), and Feilden Clegg Bradley Studios, (architects and masterplanners) to update the regeneration strategy during 2017. We have now reached a stage where we wish to consult on the new draft regeneration strategy (at the bottom of the page).. We are undertaking an eight week consultation on the draft regeneration strategy starting on 27 November 2017.. As part of the consultation, we will be holding a drop-in session on the 13 December 2017 at Block 12, Spectrum, Blackfriars Road, Salford between 1pm and 7pm (access from Blackfriars Road). This will be an opportunity to meet officers from ...
Scaffolds for bone tissue engineering lack often control of cellular instructions. We propose a triple sequential approach for customizing scaffold features from the macro to the nanoscale. The nano/meso-scale is composed by human platelet lysate and marine-origin polysaccharides assembled by layer-by-layer and shaped into fibrils by freeze-drying. We show that osteogenic induction of stem cells is tunable within a low range of layers. This approach has the potential to develop new scaffolds with enhanced cell-instructive capabilities using affordable autologous sources of bioactive molecules ...
If you have a question about this talk, please contact Anna Walczyk.. Fracture toughness has occasionally been neglected in the development of tissue engineering scaffolds. In fact, almost all recent corneal scaffolds developments aim to achieve transparent scaffolds with the tensile strength and elastic modulus closely-matched to those of native cornea despite the fact that cornea is normally subjected to below-ultimate-strength cyclic tensile loadings due to intraocular pressure, ocular muscle contractions and eye blink. Similarly to other soft collagenous tissues, toughening mechanisms in cornea are not well understood, but the lamellar structure of orthogonally aligned collagen fibrils in corneal stroma is thought to account for its toughness. To examine this, transparent laminates of gelatin nanofibers in alginate gel, mimicking the corneal lamellar structure, were created in a three-step process. First, stacks of orthogonally aligned gelatin nanofibers were created by electrospinning ...
THESIS 8757 Tissue engineering (or regenerative medicine) is defined as the application of scientific principles to the synthesis of living tissues using bioreactors, cells, scaffolds, growth factors, or a combination (Rose and Oreffo, 2002). One of the principal methods in tissue engineering involves the use of a porous scaffold to support and guide synthesis of a 3D tissue or organ (Sachlos and Czernuszka, 2003). Collagen-Glycosaminoglycan scaffolds have found success in several clinical applications of tissue engineering (Yannas et al., 1989, Chamberlain et al., 1998). ...
Bone related diseases and disorders are a significant socioeconomic burden in the United States. Autografts and allografts are most commonly used for the treatment of bone defects and non-unions; however, they are associated with limitations such as donor site morbidity and immune rejection, respectively. Over the past few decades, bone tissue engineering (BTE) using scaffold and cells has garnered significant interest as an alternative method for the repair and regeneration of bone defects. Recreation of the tissue microenvironment via the development of biomimetic scaffolds that resemble the physicochemical aspects (i.e., composition, topography, stiffness) of native bone is a promising approach that has been previously shown to improve scaffold properties and augment cellular response. In this realm, collagen type I and bioactive glass (Bioglass 45S5 (BG); an osteostimulative glass-ceramic) have been combined in numerous studies to generate hybrid scaffolds that mimic the organic and ...
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The iaxsys™ is a unique in vitro mechanobiology actuator that is compatible with established cell culture methods, consumables and incubators.. This versatile bioreactor platform facilitates near-physiologic strain of 2D membranes and scaffolds, thick 3D scaffolds (variotis™), ex vivo tissues, and soft tissue implants (eg bovine pericardium xenograft heart valve materials) within standard cell culture plates and flasks.. Quasi-static strain or cyclic strain can be applied uniformly to 6 samples simultaneously within a 6 well cell plate with a specified rate and number of cycles. Depending on the configuration, tensile or compression stresses are imparted.. When the iaxsys™ is used to actuate variotis™ scaffolds for mechanobiology studies in standard 6 well cell culture plates, the quality of RNA sampled is of a very high level. Unlike biologically derived gels and scaffolds, the fully synthetic variotis™ with b-glass™ avoids any contamination. The use of standard cell well plates ...
Fat grafting is emerging as a promising alternative to silicon implants in breast reconstruction surgery. Unfortunately, this approach does not provide a proper mechanical support and is affected by drawbacks such as tissue resorption and donor site morbidity. Synthetic scaffolds can offer a valuable alternative to address these challenges, but poorly recapitulate the biochemical stimuli needed for tissue regeneration. Here, we aim at combining the positive features of a structural, synthetic polymer to an engineered, devitalized extracellular matrix (ECM) to generate a hybrid construct that can provide a mix of structural and biological stimuli needed for adipose tissue regeneration. A RGD-mimetic synthetic scaffold OPAAF, designed for soft tissue engineering, was decorated with ECM deposited by human adipose stromal cells (hASCs). The adipoinductive potential of the hybrid ECM-OPAAF construct was validated in vitro, by culture with hASC in a perfusion bioreactor system, and in vivo, by ...
The present thesis was focused on the design and development of novel porous bioactive ceramic materials and scaffolds addressed to the regeneration of large and load-bearing bone defects. This research was carried out to meet the increasing demand for bioactive scaffolds enabling bone regeneration, due to the several drawbacks affecting the use of autologous bone, especially for large bone reconstruction. Nowadays, it is well established that effective tissue regeneration requires the implantation of scaffolds exhibiting tissue-mimicking compositional, morphological and mechanical features to promote the formation and maturation of new healthy tissue. In this context, my work was organized in three research topics on the basis of specific clinical requirements, thus leading to the development of different types of bioceramic scaffolds based on calcium phosphates, particularly: i) macroporous implants for cranio-maxillofacial defects obtained by direct foaming of ceramic suspensions, ii) 3D ...
TY - JOUR. T1 - Proliferation of genetically modified human cells on electrospun nanofiber scaffolds. AU - Borjigin, Mandula. AU - Strouse, Bryan. AU - Niamat, Rohina A.. AU - Bialk, Pawel. AU - Eskridge, Chris. AU - Xie, Jingwei. AU - Kmiec, Eric B.. PY - 2012. Y1 - 2012. N2 - Gene editing is a process by which single base mutations can be corrected, in the context of the chromosome, using single-stranded oligodeoxynucleotides (ssODNs). The survival and proliferation of the corrected cells bearing modified genes, however, are impeded by a phenomenon known as reduced proliferation phenotype (RPP); this is a barrier to practical implementation. To overcome the RPP problem, we utilized nanofiber scaffolds as templates on which modified cells were allowed to recover, grow, and expand after gene editing. Here, we present evidence that some HCT116-19, bearing an integrated, mutated enhanced green fluorescent protein (eGFP) gene and corrected by gene editing, proliferate on polylysine or ...
Abstract Chitosan-based porous scaffolds are of great interest in biomedical applications especially in tissue engineering because of their excellent biocompatibility in vivo, good texture, surface contact, controllable degradation rate and tailorable mechanical properties. These days biomaterials scaffolds have contributed as an alternative choice of therapy mainly due to the increase failure rates in autografts and allografts techniques. Terbinafine HCl is allylamines group of drugs which is used topically to treat dermatophyte group of fungi like ringworm. Chitosan possesses both anti-bacterial and antifungal property which synergises with Terbinafine HCl (TBH) for both prophylactic and therapeutic actions in treating fungal wound infection (FWI). The haemostatic property of chitosan allows sorption of plasma, erythrocyte coagulation and platelets activation. These properties contribute additional role in repairing debilitated tissue. The prolong drug release property of fabricated scaffold ...
Recent work demonstrates that osteoprogenitor cell culture on nanofiber scaffolds can promote differentiation. This response may be driven by changes in cell morphology caused by the three-dimensional (3D) structure of nanofibers. We hypothesized that nanofiber effects on cell behavior may be mediated by changes in organelle structure and function. To test this hypothesis, human bone marrow stromal cells (hBMSCs) were cultured on poly(ϵ-caprolactone) (PCL) nanofibers scaffolds and on PCL flat spuncoat films. After 1 day-culture, hBMSCs were stained for actin, nucleus, mitochondria, and peroxisomes, and then imaged using 3D confocal microscopy. Imaging revealed that the hBMSC cell body (actin) and peroxisomal volume were reduced during culture on nanofibers. In addition, the nucleus and peroxisomes occupied a larger fraction of cell volume during culture on nanofibers than on films, suggesting enhancement of the nuclear and peroxisomal functional capacity. Organelles adopted morphologies with greater 3D
Synthetic hydrogel scaffold is an effective vehicle for delivery of INFUSE (rhBMP2) to critical-sized calvaria bone defects in rats Journal Article ...
Roofing works - worker on scaffold Stock Footage. csp13353806 - silhouette of worker on the scaffold against the blue sky. Affordable Royalty Free Stock Photography. Downloads for just $2.50, with thousands of images added daily. Subscriptions available for just $39.00. Our stock photo image search engine contains royalty free photos, vector clip art images, clipart illustrations.
This book addresses important biomaterials which are commonly used to fabricate scaffolds and it describes two major protocols employed in scaffold fabrication. Tissue engineering or regenerative medi
TY - JOUR. T1 - The influence of electrically conductive and non-conductive nanocomposite scaffolds on the maturation and excitability of engineered cardiac tissues. AU - Navaei, Ali. AU - Rahmani Eliato, Kiarash. AU - Ros, Robert. AU - Migrino, Raymond Q.. AU - Willis, Brigham C.. AU - Nikkhah, Mehdi. PY - 2019/2/1. Y1 - 2019/2/1. N2 - Utilization of electrically conductive nanomaterials for developing nanocomposite scaffolds has been at the center of attention for engineering functional cardiac tissues. The primary motive in the use of conductive nanomaterials has been to develop biomimetic scaffolds to recapitulate the extracellular matrix (ECM) of the native heart and to promote cardiac tissue maturity, excitability and electrical signal propagation. Alternatively, it is well accepted that the inclusion of nanomaterials also alters the stiffness and nano-scale topography of the scaffolds. However, what is missing in the literature is that to what extent the sole presence of nanomaterials ...
We demonstrated the scaffolds full biocompatibility in vitro and safety in vivo, and preliminary results on efficacy are extremely encouraging, De Lucrezia says. Thanks to five years of EU support, we were able to progress THE GRAIL project from a simple sketch on a paper to a working prototype, paving the way for the commercial exploitation of the results.. Three SME partners in THE GRAIL have set up a dedicated spin-off company that owns the intellectual property generated in the project and will engage with larger companies capable of supporting sales. The consortium has also sought to exploit by-products of the research, for instance, testing a new technique for peripheral vascular bypass surgery.. Meanwhile, expertise gained in the project has allowed Explora to offer advanced in-vivo models for safety and efficacy testing of advanced medical devices, making the company one of the leading SMEs in Europe in the field of advanced therapy medicinal product testing.. The project partners ...
Biomedical scaffolds are described that may be used, for example, for the treatment of bone diseases and bone reconstruction and restoration. The described scaffolds having ingress and habitiaion property for cells and growth factors with serum by capillary action via engineered micro-channles. Also, the scaffolds permit nutrient and ion flow such that bone regeneration in the area surrounding the scaffold is promoted. Kits that include such scaffolds and methods of preparing and using such scaffolds are also provided.
The present invention relates to biomimetic scaffolds, methods for making the same, and methods for using the same. The scaffolds comprise a plurality of graded or tapered microchannels that provide s
Each scaffold was placed aseptically into a well of a non-tissue culture treated 24-well (in the semiconfocal Raman study) or 48-well plate (in the confocal and fibre-optic Raman study; both Nunc, Thermo Fisher Scientific, Roskilde, Denmark).. In the semiconfocal Raman study, human fibronectin (300 µg ml−1, BD Biosciences), which is known to support cell adhesion [30], was applied to the chondrocyte suspension before seeding onto the scaffolds. The cell-fibronectin suspension, 3 × 106 cells in a final volume of 50 µl for each scaffold, was pipetted onto scaffolds and left to completely adsorb into the porous structure. Samples were left in an incubator at 37°C under a humidified atmosphere of 5% CO2 for 45 min. Subsequently, 2 ml of culture medium was carefully added drop wise. After 7 and 21 days of culture, the samples were studied using Raman microspectroscopy. Chondrocytes of C-28/I2 human immortalized chondrocyte cell line were also seeded on PEOT/PBT scaffolds. C-28/I2 cells were ...
MyJournals.org - Science - IJMS, Vol. 19, Pages 3148: Screening of Additive Manufactured Scaffolds Designs for Triple Negative Breast Cancer 3D Cell Culture and Stem-Like Expansion (International Journal of Molecular Sciences)
Abstract A computational structural deterministic modeling strategy has been developed and experimentally validated to (1) assist tissue engineering scaffold fabrication, and as a consequence to improve in vivo scaffolds performances, and (2) provide a better understanding of cellular mechanical and metabolic response to local micro-structural deformations of the extracellular matrix (ECM). Image analysis software was developed and tested on electrospun poly (ester urethane) urea (PEUU) scaffolds, collagen gels, decellularized tissues. The algorithm analyzed SEM and multi-photon images (maximum imaging penetration depth: 160 µm) providing a full 3D characterization of engineered constructs morphology (n ≥ 6). The detected material topologies were adopted to generate statistically equivalent scaffold biomechanical models minimizing the difference between the real material and network model architectural features. The mechanical response at the macro scale was fully characterized by stress control
"Porous three-dimensional carbon nanotube scaffolds for tissue engineering". Journal of Biomedical Materials Research Part A. ... "Collagen-carbon nanotube composite materials as scaffolds in tissue engineering". Journal of Biomedical Materials Research Part ... all-carbon scaffolds using single- and multi-walled carbon nanotubes as building blocks.[5] These scaffolds possess macro-, ... Riverside have shown that carbon nanotubes and their polymer nanocomposites are suitable scaffold materials for bone tissue ...
... scaffolds are used in bone tissue engineering to mimic the natural extracellular matrix of the bones. The bone tissue ... "Ligament tissue engineering using synthetic biodegradable fiber scaffolds". Tissue Engineering. 5 (5): 443-52. doi:10.1089/ten. ... Using silk scaffolds as a guide for growth for bone tissue regeneration, Kim et al. observed complete bone union after 8 weeks ... These scaffolds can be used to deliver bioactive agents that promote tissue regeneration. These bioactive materials should ...
... glycosaminoglycan scaffolds". Journal of Tissue Engineering and Regenerative Medicine. 13 (2): 261-273. doi:10.1002/term.2789. ... PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent ... The addition of PDGF at specific time‐points has been shown to stabilise vasculature in collagen‐glycosaminoglycan scaffolds.[ ... During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in ...
Elastic tissue is classified as "connective tissue proper".[2] The elastic fiber is formed from the elastic microfibril ( ... The microfibril scaffolds and organizes the deposition of amorphous elastin. Amorphous elastin forms from monomers of soluble ... Elastic fibers (or yellow fibers) are bundles of proteins (elastin) found in extracellular matrix[1] of connective tissue and ... Elastosis is the buildup of elastic fibers in tissues, and is a form of degenerative disease.[5] There are a multitude of ...
Each type of connective tissue in animals has a type of ECM: collagen fibers and bone mineral comprise the ECM of bone tissue; ... Hadjipanayi E, Mudera V, Brown RA (February 2009). "Close dependence of fibroblast proliferation on collagen scaffold matrix ... The ECM can exist in varying degrees of stiffness and elasticity, from soft brain tissues to hard bone tissues. The elasticity ... Sound Medicine - Heart Tissue Regeneration - July 19 interview discussing ECM and its uses in cardiac tissue repair (requires ...
Microfluidic scaffolds for tissue engineering. Nature Materials (2007) vol. 6 pp. 908-915. ...
"Chitin scaffolds in tissue engineering". Int J Mol Sci. 12 (3): 1876-87. doi:10.3390/ijms12031876. PMC 3111639. PMID 21673928. ... Additionally, keeping in mind the size and shape of the final tissue, the potential of the physical dimensions of the tissue of ... Similarly, whole organs can be decellularized to create 3-D ECM scaffolds. These scaffolds can then be re-cellularized in an ... the ECM biomaterial retains some characteristics of the original tissue. The ECM tissues can be harvested from varying stages ...
Microfluidic scaffolds for tissue engineering. Nature Materials (2007) vol. 6 pp. 908-915 Bernard, Claude. Introduction à ...
"Biodegradable Polymer Scaffolds for Tissue Engineering". Bio/Technology. 12 (7): 689-693. doi:10.1038/nbt0794-689. PMID 7764913 ... Macromolecules tend to be broken down by digestion and blocked by body tissues if they are injected or inhaled, so finding a ... He and the researchers in his lab have made advances in tissue engineering, such as the creation of engineered blood vessels ... He is a widely recognized and cited researcher in biotechnology, especially in the fields of drug delivery systems and tissue ...
... and scaffolds for the production of artificial human tissues. These scaffolds fulfill a similar purpose as the extracellular ... Electrospinning can also be used for medical purposes.[51] The electrospun scaffolds made for tissue engineering applications ... A novel scaffold for tissue engineering". Journal of Biomedical Materials Research. 60 (4): 613-621. doi:10.1002/jbm.10167.. ... This early application of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types ...
Scaffolds in tissue engineering. When used as scaffolds, hydrogels may contain human cells to repair tissue. They mimic 3D ... This technique has seen considerable use in cell and tissue engineering applications due to the ability to inject or mold a ... Discher, D. E.; Janmey, P.; Wang, Y.L. (2005). "Tissue Cells Feel and Respond to the Stiffness of Their Substrate" (PDF). ... Hydrogels also possess a degree of flexibility very similar to natural tissue, due to their significant water content. As ...
PMID 17584904.CS1 maint: multiple names: authors list (link) D.W. Hutmacher (2000). "Scaffolds in tissue engineering bone and ... S.W. Kim, H.-D. Jung, M.-H. Kang, H.-E. Kim, Y.-H. Koh, Y. Estrin (2013). "Fabrication of porous titanium scaffold with ... It has been shown that a macropore size of 200-500 µm is preferred for ingrowths of new bone tissues and transportation of body ... As a result of this imbalance, the starting bone density will be reduced, there will be tissue death and, eventually, implant ...
"Fibrochondrogenesis of free intraarticular small intestinal submucosa scaffolds". Tissue Engineering. 10 (1-2): 129-37. doi: ... bone and connective tissues. Overwhelming disorganization of cellular processes involved in the formation of cartilage and bone ... specialized cells that make up fibrous connective tissue, which plays a role in the formation of cellular structure and ... causing abnormal fibrous development of cartilage and related tissues. It is a lethal rhizomelic (malformations which result in ...
These can then be used to re-cellularise a three-dimensional lung tissue scaffold. Indeed, in 2008, there was a successful ... In humans with non-injured tissues, the tissue is naturally regenerated over time; by default these tissues have new cells ... Though tissues with a non-interrupted morphology, like non-injured soft tissue, completely regenerate consistently; the ... Therefore, to regenerate hollow organs and tissues with a long diffusion distance, the tissue had to be regenerated inside the ...
"Porous three-dimensional carbon nanotube scaffolds for tissue engineering". Journal of Biomedical Materials Research Part A. ... These 3D graphene (all-carbon) scaffolds/foams have potential applications in fields such as energy storage, filtration, ... "Fabrication and characterization of three-dimensional macroscopic all-carbon scaffolds". Carbon. 53: 90-100. doi:10.1016/j. ...
"Porous three-dimensional carbon nanotube scaffolds for tissue engineering". Journal of Biomedical Materials Research Part A. ... These 3D graphene (all-carbon) scaffolds/foams have applications in several fields such as energy storage, filtration, thermal ... "Fabrication and characterization of three-dimensional macroscopic all-carbon scaffolds". Carbon. 53: 90-100. doi:10.1016/j. ... "Two-dimensional nanostructure-reinforced biodegradable polymeric nanocomposites for bone tissue engineering". Biomacromolecules ...
"Tissue regeneration in vivo within recombinant spidroin 1 scaffolds". Biomaterials. 33 (15): 3887-98. doi:10.1016/j. ... could be used in medicine without risk of biocompatibility issues and thus potentially lead to many new opportunities in tissue ...
Matrices and Scaffolds for Drug Delivery in Tissue Engineering. 59 (4-5): 263-273. doi:10.1016/j.addr.2007.03.013. PMID ... "Injectable matrices and scaffolds for drug delivery in tissue engineering". Advanced Drug Delivery Reviews. ... The described behavior can be exploited in tissue engineering since the adhesion of cells is strongly dependent on the ... Promising areas of application are tissue engineering, liquid chromatography, drug delivery and bioseparation. Only a few ...
"Porous three-dimensional carbon nanotube scaffolds for tissue engineering". Journal of Biomedical Materials Research Part A. ... all-carbon scaffolds using single- and multi-walled carbon nanotubes as building blocks. These scaffolds possess macro-, micro ... all-carbon scaffolds using single- and multi-walled carbon nanotubes as building blocks. These scaffolds possess macro-, micro ... These 3D all-carbon scaffolds/architectures may be used for the fabrication of the next generation of energy storage, ...
Because of this, they are of great interest for gene delivery and tissue engineering scaffolds. Most hydroxyapatite ceramics ... These, in turn, compose the basic proteins, which are the primary constituents of the 'soft tissues' common to most biominerals ... Orthopaedic implants made from these materials bond readily to bone and other tissues in the body without rejection or ...
... glycosaminoglycan scaffolds". Journal of Tissue Engineering and Regenerative Medicine. 13 (2): 261-273. doi:10.1002/term.2789. ... The hypoxia that is noted in these areas causes the tissues to demand the presence of nutrients and oxygen that will allow the ... The presence of blood vessels where there should be none may affect the mechanical properties of a tissue, increasing the ... The absence of blood vessels in a repairing or otherwise metabolically active tissue may inhibit repair or other essential ...
"Tissue engineering of complex tooth structures on biodegradable polymer scaffolds". J Dent Res. 81 (10): 695-700. doi:10.1177/ ... Biopolymer methods in tissue engineering Hill, David J. (2012-05-10). "Toothless no more - Researchers using stem cells to grow ... Tooth regeneration is a stem cell based regenerative medicine procedure in the field of tissue engineering and stem cell ...
Radisic developed a flexible shape-memory scaffold for minimally invasive delivery of functional tissues. The scaffold utilizes ... Biomimetic approach to cardiac tissue engineering: oxygen carriers and channeled scaffolds. Tissue Engineering, 12(8), pp. 2077 ... "Moldable elastomeric polyester-carbon nanotube scaffolds for cardiac tissue engineering". "Flexible shape-memory scaffold for ... e18 Flexible shape-memory scaffold for minimally invasive delivery of functional tissues M Montgomery, S Ahadian, LD Huyer, ML ...
Gerhardt, Lutz-Christian (2010). "Bioactive Glass and Glass-Ceramic Scaffolds for Bone Tissue Engineering". Materials. 3 (7): ... with foam-like scaffolds being introduced to maximize the interfacial area between the implant and body tissue. One issue that ... Glass-ceramics are used in medical applications due to their unique interaction, or lack thereof, with human body tissue. ... Resorbable ceramics are intended to gradually dissolve entirely, all the while new tissue grows in its stead. The architecture ...
... seeding of polymer scaffolds for cartilage tissue engineering, cultivation parameters, and tissue construct characterization, ... The focus of her research is on engineering functional human tissues, by an integrated use of stem cells, biomaterial scaffolds ... "Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering". Biotechnology Progress. 14 (2): 193-202. doi: ... "Cardiac tissue engineering: Cell seeding, cultivation parameters, and tissue construct characterization". Biotechnology and ...
Scaffolds act as three-dimensional artificial templates in which the tissue targeted for reconstruction is cultured to grow ... Scaffolds are subject to harsh processing conditions during tissue culturing. These include mechanical stimulation to promote ... Current research is exploring the effectiveness of using various types of hydrogel scaffolds for tissue engineering and ... This high water content makes hydrogel more similar to living body tissues than any other material for tissue regeneration. ...
Tissue-engineered nerve guidance conduits are a combination of many elements: scaffold structure, scaffold material, cellular ... In tissue engineering, the three main levels of scaffold structure are considered to be: the superstructure, the overall shape ... Thus, aragonite scaffolds may be useful for nerve tissue repair and regeneration. It is hypothesized that aragonite-derived ... The superstructure of a conduit or scaffold is important for simulating in vivo conditions for nerve tissue formation. The ...
This setback makes it more difficult for the degradation rate of the scaffold to coincide with the rate of tissue formation. ... Touri, R (September 2013). "The Use of Carbon Nanotubes to Reinforce 45S5 Bioglass-Based Scaffolds for Tissue Engineering". ... Chen, Q.; Thompson, I.; Boccaccini, A. (2006). "45S5 Bioglass®-derived glass-ceramic scaffolds for bone tissue engineering". ... A setback to using Bioglass 45S5 is that it is difficult to process into porous 3D scaffolds. These porous scaffolds are ...
Also as an example, the use of silicon nanowires in nanoporous materials to create scaffolds for synthetic tissues allows for ... "Macroporous nanowire nanoelectronic scaffolds for synthetic tissues". Nature Materials. 11 (11): 986-994. Bibcode:2012NatMa..11 ... a way to create a synthetic tissue structure that could be used to monitor the electrical activity of the cells on the scaffold ... A biointerface is the region of contact between a biomolecule, cell, biological tissue or living organism or organic material ...
... stem cells in adult bone marrow and other tissues". Leukemia 21 (5): 860-7. PMID 17344915. ... of Spinal Cord Injury Following Application of Intralesional Bone Marrow Mononuclear Cells Embedded in Polymer Scaffold - Two ... "Stem-cell therapy shows promise for horse soft-tissue injury, disease". DVM Newsmagazine. Vaadatud 2013-10-21 ...
Lee, M.S.Y. (2013). "Turtle origins: Insights from phylogenetic retrofitting and molecular scaffolds". Journal of Evolutionary ... Morphological and cellular aspects of tail and limb regeneration in lizards a model system with implications for tissue ... via connective tissue) to the rest of the viscera. By using a series of special muscles (roughly equivalent to a diaphragm), ...
... balloon-like lesions that may be filled with air or pus or scar tissue) and very high concentrations of the serum antibody IgE ... 4) β-Scaffold factors. with minor groove contacts. 4.2. *Hyperimmunoglobulin E syndrome ...
"Bromine Is an Essential Trace Element for Assembly of Collagen IV Scaffolds in Tissue Development and Architecture". Cell. 157 ... Possibly important to basement membrane architecture and tissue development, as a needed catalyst to make collagen IV.[37] ...
October 2005). "Meniscal regeneration using tissue engineering with a scaffold derived from a rat meniscus and mesenchymal ... These cells are held in place by a small piece of soft tissue from the tibia, called a periosteal flap, which is sutured over ... This drives efforts to develop ways of using a person's own cells to grow, or re-grow cartilage tissue to replace missing or ... For years, the concept of harvesting stem cells and re-implanting them into one's own body to regenerate organs and tissues has ...
skeletal muscle tissue development. · embryo development. · post-embryonic development. · cerebellum development. · caudate ... smooth muscle tissue development. · regulation of sequence-specific DNA binding transcription factor activity. · righting ... 4) β-Scaffold factors with minor groove contacts. (4.1) Rel homology region(英语:Rel homology domain) ...
... , C20orf32, CAS4, HEFL, HEPL, Cas scaffolding protein family member 4, Cas scaffold protein family member 4. ... the CASS4 transcripts are most highly expressed in spleen and lung among normal tissues, and are highly expressed in ovarian ...
... or adipose tissue deposition in the breasts without true glandular tissue, a trait that is not indicative of pubertal ... 4) β-Scaffold factors. with minor groove contacts. 4.2. *Hyperimmunoglobulin E syndrome ... exclude the possibility that some residual estrogen sensitivity could be present in some tissues", which is in accordance with ...
"Bromine Is an Essential Trace Element for Assembly of Collagen IV Scaffolds in Tissue Development and Architecture". Cell. 157 ... Hillyer, Julián F.; Albrecht, Ralph M. (2001). "Gastrointestinal persorption and tissue distribution of differently sized ...
This can happen anywhere in the body, such as the skin, breast tissue, or lung. This type of tumor can often, depending on ... frequently from scaffolds, after the building has been erected.[7][8] Also commonly used to describe the site specific dance ...
There is also poly(L-lactide-co-D,L-lactide) (PLDLLA) - used as PLDLLA/TCP scaffolds for bone engineering.[25][26] ... Bose, S.; Vahabzadeh, S.; Bandyopadhyay, A. (2013). "Bone tissue engineering using 3D printing". Materials Today. 16 (12): 496- ... Thanks to its bio-compatibility and biodegradability, PLA has also found ample interest as a polymeric scaffold for drug ... Pavia FC; La Carrubba V; Piccarolo S; Brucato V (August 2008). "Polymeric scaffolds prepared via thermally induced phase ...
Discs large homolog 1 (DLG1), also known as synapse-associated protein 97 or SAP97, is a scaffold protein that in humans is ... tissue morphogenesis. • reproductive structure development. • membrane raft organization. • hard palate development. • ... protein complex scaffold activity. • protein kinase binding. • L27 domain binding. • ligand-gated ion channel activity. • ... smooth muscle tissue development. • negative regulation of epithelial cell proliferation. • regulation of sodium ion ...
Autografts (employing bone or tissue harvested from the patient's body). *Allografts (using bone or tissue from another body, ... Mechanical properties of the scaffolds are further enhanced through braiding and twisting of the scaffold materials. ... In order for MSCs to differentiate into an ACL, they must be placed in a proper scaffold on which to grow, and must be in a ... Synthetic tissue for ACL reconstruction has also been developed, but little data exists on its strength and reliability.[ ...
The tissue, animal model, and animal and human genetic studies cited above implicate ALOX5 in a wide range of diseases: a) ... Based on in vitro studies, this protein binding serves to stabilize ALOX5 by acting as a Chaperone (protein) or scaffold, ... 5-HEPE, 5-oxo-HEPE, LTB5, LTC5, LTD5, and LTE5 are generally less potent in stimulating cells and tissues than their ... Furthermore, the ALOX5-related tissue reactions studied to date are influenced by multiple genetic, environmental, and ...
Cycloalkanol ethylamine scaffold[edit]. Venlafaxine and desvenlafaxine contain a cycloalkanol ethylamine scaffold. Increasing ... moderate tissue distribution and a reasonably long elimination half-life. Milnacipran's lack of drug-drug interactions via ... Aryloxypropanamine scaffold[edit]. Several reuptake inhibitors contain an aryloxypropanamine scaffold. This structural motif ... The application of an aryloxypropanamine scaffold has generated a number of potent MAOIs.[33] Before the development of ...
... "bimodal tissue scaffold", where both micron-scale and nano-scale fibers were deposited simultaneously.[13] Scaffolds made via ... Tissue Engineering[edit]. Melt electrospinning is used to process biomedical materials for tissue engineering research. ... Dalton PD, Jörgensen N, Groll J, Möller M (2008) Patterning of melt electrospun substrates for tissue engineering. Biomed Mater ... Not using solvents to process a polymer assists in tissue engineering applications where solvents are often toxic. Additionally ...
Origin and subsequent variaton and development of neurons and neural tissues and organs ... "A post-synaptic scaffold at the origin of the animal kingdom". PLoS ONE. 2 (6): e506. Bibcode:2007PLoSO...2..506S. doi:10.1371 ...
Researchers at Tufts developed scaffolds made of spongy silk that feel and look similar to human tissue. They are implanted ... during reconstructive surgery to support or restructure damaged ligaments, tendons, and other tissue. They also created ...
The structural role of the residues left by the dead cells is reminiscent of the products of PCD in plant tissue. D. discoideum ... and the scaffold protein FIP200. Class III PI3K complex, containing hVps34, Beclin-1, p150 and Atg14-like protein or ... In "APL regulates vascular tissue identity in Arabidopsis", Martin Bonke and his colleagues had stated that one of the two long ... The concept of "programmed cell-death" was used by Lockshin & Williams in 1964 in relation to insect tissue development, around ...
... a stent may be passed first into the parent artery to serve as a scaffold for the coils.[citation needed] ... Genetic conditions associated with connective tissue disease may also be associated with the development of aneurysms.[5] This ...
Effects of donor's age, tissue, and genotype". Laboratory Investigation. 23 (1): 86-92. PMID 5431223.. ... by acting as a scaffold protein that forms a filamentous meshwork underlying the inner nuclear envelope, the membrane that ... Misrepair-accumulation aging theory[88][89] suggests that the abnormality of tissue structure is the common point between ... They affect only one tissue and can be classified as unimodal progeroid syndromes. Segmental progeria, which is more frequently ...
Implantation of stem cells from culture into an existing tissue structure Implantation of stem cells into a tissue scaffold ... namely repair of the damage that is associated with aging or replacement of damaged tissue with new tissue. Rejuvenation can be ... For other tissues it needs various growth factors to stimulate cell division, or in some cases it needs stem cells. Senescent ... Of course animal cells are not able to be included in human tissue, but they can secrete factors for rejuvenating. That's why ...
1c has also been shown to upregulate in a tissue specific manner the expression of PGC1alpha expression in brown adipose tissue ... 4) β-Scaffold factors with minor groove contacts. (4.1) Rel homology region. *NF-κB *NFKB1 ...
Scanziani E (1998). "Immunohistochemical staining of fixed tissues". Methods Mol Biol. 104: 133-140. doi:10.1385/0-89603-525-5: ... "Engineered protein scaffolds as next-generation antibody therapeutics". Curr Opin Chem Biol. 13 (3): 245-255. doi:10.1016/j. ... Milland J, Sandrin MS (December 2006). "ABO blood group and related antigens, natural antibodies and transplantation". Tissue ... Common advantages over antibodies are better solubility, tissue penetration, stability towards heat and enzymes, and ...
"A Post-Synaptic Scaffold at the Origin of the Animal Kingdom". PLOS ONE. 2 (6): e506. doi:10.1371/journal.pone.0000506. PMC ... The amount of tissue that needs food and oxygen is determined by the volume, but the pumping capacity that supplies food and ... Unlike other animals, they lack true tissues and organs, and have no body symmetry. The shapes of their bodies are adapted for ... Adult sponges lack neurons or any other kind of nervous tissue. However most species have the ability to perform movements that ...
... hyaluronan is gaining popularity as a biomaterial scaffold in tissue engineering research.[77][78][79] In particular, a number ... Granulation tissue is the perfused, fibrous connective tissue that replaces a fibrin clot in healing wounds. It typically grows ... The initial granulation tissue formed is highly inflammatory with a high rate of tissue turnover mediated by matrix degrading ... Cell migration is essential for the formation of granulation tissue.[26] The early stage of granulation tissue is dominated by ...
... scaffolds with epidermal growth factor for neural tissue engineering". Biomatter. 6 (1): e1231276. doi:10.1080/21592535.2016. ... EGF has since been found in many human tissues including submandibular gland (submaxillary gland) and parotid gland.[7] ... These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy to bone tissue engineering ... EGF is used to modify synthetic scaffolds for manufacturing of bioengineered grafts by emulsion electrospinning or surface ...
A 37 °C folding efficiency (F64L) point mutant to this scaffold, yielding enhanced GFP (EGFP), was discovered in 1995 by the ... "Fluorescent proteins and their applications in imaging living cells and tissues". Physiological Reviews. 90 (3): 1103-63. doi: ... is nonfluorescent in the absence of the properly folded GFP scaffold and exists mainly in the un-ionized phenol form in wtGFP.[ ... transgenic rats display high expression in most tissues, and many cells that have not been characterized or have been only ...
These scaffold proteins are thought to be the constituents of the dense pyramid like structures of the active zone and are ... Tissue Types. *Grey matter. *White matter *Projection fibers. *Association fiber. *Commissural fiber ... There are at least five major scaffold proteins that are enriched in the active zone; UNC13B/Munc13, RIMS1 (Rab3-interacting ... 2004). "Dap160/Intersectin Scaffolds the Periactive Zone to Achieve High-Fidelity Endocytosis and Normal Synaptic Growth". ...
Tissue scaffold technology could help rebuild large organs. An artists impression of myoglobin-based artificial membrane ... Scientists have developed a new tissue scaffold technology that could one day enable the engineering of large organs. ... of Bristol and Liverpool has shown that it is possible to combine cells with a special scaffold to produce living tissue in the ... "Creating larger pieces of cartilage gives us a possible way of repairing some of the worst damage to human joint tissue, such ...
... based tissue engineering scaffolds. High diffusivities facilitate the supply of oxygen and nutrients to... ... The design of scaffolds for use in tissue engineering-traditional factors. Tissue Eng. 2001;7:679-89.CrossRefGoogle Scholar ... based tissue engineering scaffolds. High diffusivities facilitate the supply of oxygen and nutrients to grown tissue as well as ... Scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000;21(24):2529-43.CrossRefGoogle Scholar ...
Active Implants and Scaffolds for Tissue Regeneration. Editors. * Meital Zilberman Series Title. Studies in Mechanobiology, ... 2. Scaffolds for bone regeneration. 3. Scaffolds based on natural polymers 4. New polymeric systems for active implants The 18 ... This effect is achieved by controlled release of bioactive agents to the surrounding tissue. This book presents a broad ... Chitosan-Derivative Based Hydrogels as Drug Delivery Platforms: Applications in Drug Delivery and Tissue Engineering ...
Tissue engineering scaffolds are designed to support tissue self-healing within physiological environments by promoting the ... Oxygen Diffusion Bioactive Glass Tissue Engineering Scaffold Strut Thickness Tissue Phase These keywords were added by machine ... Tissue engineering scaffolds are designed to support tissue self-healing within physiological environments by promoting the ... Optimising bioactive glass scaffolds for bone tissue engineering. Biomaterials. 2006;27:964-73.CrossRefGoogle Scholar ...
Scaffold Porosity. The porosity of the scaffold was measured by liquid displacement, as calculated according to , where is the ... Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering. Hui Fan,1,2 Junfeng Hui,1,2 Zhiguang Duan,1,2 Daidi ... D. W. Hutmacher, "Scaffolds in tissue engineering bone and cartilage," Biomaterials, vol. 21, no. 24, pp. 2529-2543, 2000. View ... is the dry weight of the scaffold, is the density of the dehydrated alcohol, and is the volume of the scaffold. Three parallel ...
As of yet, these scaffolds cannot electrically probe the physicochemical and biological microenvironments throughout … ... synthetic biomaterials as structural and bioactive scaffolds is central to fields ranging from cellular biophysics to ... Macroporous nanowire nanoelectronic scaffolds for synthetic tissues Nat Mater. 2012 Nov;11(11):986-94. doi: 10.1038/nmat3404. ... 3D macroporous nanoES mimic the structure of natural tissue scaffolds, and they were formed by self-organization of coplanar ...
... Cijun Shuai,1,2 Yiyuan Cao,1 Chengde Gao,1 ... Q. Fu, E. Saiz, M. N. Rahaman, and A. P. Tomsia, "Bioactive glass scaffolds for bone tissue engineering: state of the art and ... I. Zein, D. W. Hutmacher, K. C. Tan, and S. H. Teoh, "Fused deposition modeling of novel scaffold architectures for tissue ... The results of all these studies indicated that HANw reinforced 63s glass scaffolds could be an ideal candidate for bone tissue ...
However, there are other aspects of tissue engineering where the presence of a scaffold may be beneficial:. • Scaffolds provide ... Porous scaffold design for tissue engineering. Nat. Mater. 4, 518 (2005). 10.1038/nmat1421pmid:16003400 doi:10.1038/nmat1421. ... Smart materials as scaffolds for tissue engineering. J. Cell. Physiol. 203, 465 (2005). 10.1002/jcp.20270pmid:15744740 doi: ... Scaffolds in tissue engineering bone and cartilage. Biomaterials 21, 2529 (2000). 10.1016/S0142-9612(00)00121-6pmid:11071603 ...
... The analyst has been monitoring the tissue scaffolds market and it is poised to grow ... The tissue scaffolds market is segmented as below:. By Product. • Natural Tissue Scaffolds. • Synthetic Tissue Scaffolds. By ... Our report on tissue scaffolds market covers the following areas:. • Tissue scaffolds market sizing. • Tissue scaffolds market ... Global Tissue Scaffolds Market 2021-2025. The analyst has been monitoring the tissue scaffolds market and it is poised to grow ...
In one aspect, the invention provides a porous metal scaffold comprising a porous metal network having pores defined by metal ... In other aspects, the invention provides methods of forming porous scaffolds. In one such aspect, the method includes providing ... In yet other aspects, the invention provides methods of improving stability of porous scaffolds. ... The porous scaffold facilitates in-growth of bone tissue into the pores of the scaffold, contributing to a long useful life of ...
... of Bristol and Liverpool has shown that it is possible to combine cells with a special scaffold to produce living tissue in the ... scaffold technology that could one day enable the engineering of large organs. Research led by the universities ... Tissue scaffold technology could help rebuild large organs. University of Bristol. Journal. Nature Communications. Keywords. ... Scientists have developed a new tissue scaffold technology that could one day enable the engineering of large organs. ...
Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Jonathan M. Brunger, Nguyen P. T. ... Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage Message Subject (Your Name) has sent ... The ability to develop tissue constructs with matrix composition and biomechanical properties that promote rapid tissue repair ... Scaffold-mediated transduction of MSCs. Jonathan M. Brunger, Nguyen P. T. Huynh, Caitlin M. Guenther, Pablo Perez-Pinera, ...
Biodegradable porous polyurethanes scaffold have themselves opportunities in service, in-cluding controlled degradation rate, ... "Biodegradable and bioactive porous polyurethanes scaffolds for bone tissue engineering" written by Mei-Na Huang, Yuan-Liang ... Synthesis of poly (ε-caprolactone) based polyurethane semi-interpenetrating polymer networks as scaffolds for skin tissue ... Synthesis of poly (ε-caprolactone)-based polyurethane semi-interpenetrating polymer networks as scaffolds for skin tissue ...
... reader is introduced to the concept of NTP and its application in plasma-assisted surface modification of nanofibrous scaffolds ... This paper provides a comprehensive overview of nanofibrous structures for tissue engineering purposes and the role of non- ... Fabrication and Plasma Modification of Nanofibrous Tissue Engineering Scaffolds by Mahtab Asadian 1,*, Ke Vin Chan 1, Mohammad ... "Fabrication and Plasma Modification of Nanofibrous Tissue Engineering Scaffolds." Nanomaterials 10, no. 1: 119. ...
In this review we summarize the current developments on scaffold systems available for cartilage tissue engineering. The ... and fabricated forms of the scaffolds (three-dimensional scaffolds, micro- or nanoparticles, and their composites). In the end ... to provide recommendations on the combination of qualities that would produce the ideal scaffold system for cartilage tissue ... The lack of efficient treatment modalities motivates researches into cartilage tissue engineering such as combing cells, ...
... and biodegradable scaffold that permits cell adherence and proliferation, allows preservation of cell-specific properties, and ... Biodegradable honeycomb collagen scaffold for dermal tissue engineering J Biomed Mater Res A. 2008 Dec 15;87(4):1103-11. doi: ... Tissue engineering requires a mechanically stable, biocompatible, and biodegradable scaffold that permits cell adherence and ... cultures of human skin fibroblasts and characterized as an effective and suitable scaffold for dermal tissue engineering. About ...
A new study describes the development of a novel hybrid polymer suitable for producing 3-D-printed scaffolds on which living ... cells can be seeded to create engineered tissues. The ability to use these hybrid polymer spools with easy-to-operate, ... A novel hybrid polymer simplifies 3-D printing of scaffolds for tissue engineering. Mary Ann Liebert, Inc./Genetic Engineering ... A novel hybrid polymer simplifies 3-D printing of scaffolds for tissue engineering ...
... depend in part on preparing a tissue scaffold from a natural tissue matrix. An essential step in tissue scaffold fabrication is ... Decellularizing Tissue Engineering Scaffolds with Carbon Dioxide Many strategies for tissue engineering of replacement ... Compressed carbon dioxide (CO2) for decontamination of biomaterials and tissue scaffolds. Pedro J. Tarafa, Aidaris Jiménez, ... We are evaluating a novel decellularization method using liquid or supercritical carbon dioxide that will prevent tissue ...
665c) Self-Assembled Peptides with RGD Motifs As Scaffolds for Tissue Engineering. ... Self-assembled peptides gain increasing interest as biocompatible and biodegradable scaffolds for tissue engineering (1). ... Self-assembled proteins and peptides as scaffolds for tissue regeneration. Advanced Healthcare Materials 16: 2557-86. ... that are amenable to computational design offer open-ended possibilities towards multifunctional tissue engineering scaffolds ...
... (Received ... Title Fabrication and Characterization of Gelatin/Carbon Black-Based Scaffolds for Neural Tissue Engineering Applications. ... as scaffolds for neural tissue engineering applications. The presented hydrogel constructs were fabricated in the form of films ... Neural tissue engineering has recently emerged as an alternative strategy to repair nerve damage and promote nerve regeneration ...
Review of polymer-based scaffolds revealed that a composite scaffold formed by copolymerization is more effective than single ... Methods of TE have advanced significantly in recent years, but there are challenges to using engineered tissues and organs ... Analysis of biomaterials used as scaffolds may, however, elucidate how TE can be enhanced. Ideally, biomaterials should closely ... offers a potential solution for the shortage of transplantable organs and the need for novel methods of tissue repair. ...
The field of Tissue Engineering is currently limited in the range of biomaterials available for development of tissue ...
A second effort in this thesis addressed the potential separation of the scaffold from the tissue (loss of apposition) due to ... One of the most important aspects of tissue engineering related to material science is design of the polymer scaffolds. The ... engineering and surgery to enable growth of new living tissues on scaffolding constructed from implanted polymeric materials. ... polymer scaffolds needs to have some specific mechanical strength over certain period of time. In this work bioresorbable ...
Scaffolds in bone tissue engineering - evolution of a concept. Attempts at tissue engineering of bone long precede the current ... The use of mineralized scaffolds for guiding bone tissue engineering has revealed unexpected manners in which the scaffold and ... Scaffolds and skeletal stem cells - mimicking physiology in tissue engineering. The recognition of the existence of skeletal ... Both in natural settings and in bone tissue engineering, bone cells handle a scaffold surface in a similar manner. They deposit ...
The thesis first focused on the development and characterisation of the tissue scaffold. A non-woven fibrous scaffold was ... To overcome these challenges, this thesis is focused on the development of a novel biomimetic tissue scaffold concept ... by incorporating growth factors into tissue scaffolds. While growth factors are crucial cell-inducing components, their ... The field of tissue engineering has advanced and evolved to focus on biomimetic strategies to meet the rise in demands of ...
Two kinds of novel biodegradable porous scaffolds for tissue engineering have been developed by our group by hybridizing ... The hybrid scaffolds will be useful for tissue engineering. ... The hybrid scaffolds were used for three-dimensional culture of ... cartilage and osteochondral tissue. These cells adhered and spread well in the hybrid scaffolds, proliferated, secreted ... The synthetic polymer sponge, or mesh serving as a skeleton, reinforced the hybrid scaffolds and resulted in easy handling, ...
Laser microfabricated poly(glycerol sebacate) scaffolds for heart valve tissue engineering.. [Nafiseh Masoumi, Aurélie Jean, ... Microfabricated poly(glycerol sebacate) (PGS) scaffolds may be applicable to tissue engineering heart valve leaflets by virtue ... This study provides a basis for the computational design of biomimetic microfabricated PGS scaffolds for tissue-engineered ... Scaffolds cultivated with fibroblasts for 3 weeks accumulated collagen (736 ± 193 μg/g wet weight) and DNA (17 ± 4 μg/g wet ...
Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering. Research and Teaching Output of the MIT ... Laminin coated scaffolds promoted the attachment of NSCs to the scaffold struts and resulted in a more even distribution of ... Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering. Download ... Rat neural stem cells (NSCs) were cultured in monolayer or in porous collagen scaffolds and exposed to neurogenic or non- ...
About Tissue Scaffolds Tissue scaffolds are cell growth supporting structures composed of natural or synthetic biocompatible ... Global Tissue Scaffolds Market 2018-2022 Global Tissue Scaffolds Market 2018-2022 ... Global Tissue Scaffolds Market 2018-2022. About Tissue Scaffolds. Tissue scaffolds are cell growth supporting structures ... Tissue scaffolds market in Americas*Table Tissue scaffolds market in Americas - Market size and forecast 2017-2022 ($ mn) ...
  • The ability to develop tissue constructs with matrix composition and biomechanical properties that promote rapid tissue repair or regeneration remains an enduring challenge in musculoskeletal engineering. (pnas.org)
  • The ability to induce and maintain differentiation of stem cells in situ could bypass these steps and enhance the success of engineering approaches for tissue regeneration. (pnas.org)
  • The factors that are critical to successfully design an ideal scaffold for cartilage regeneration were discussed. (ingentaconnect.com)
  • 1. Loo Y, Goktas M, Tekinay AB, Guler MO, Hauser CAE, Mitraki A. (2015) Self-assembled proteins and peptides as scaffolds for tissue regeneration. (aiche.org)
  • Neural tissue engineering has recently emerged as an alternative strategy to repair nerve damage and promote nerve regeneration. (astm.org)
  • It involves the fabrication of scaffolds with properties mimicking those of the natural extracellular matrix for guiding a three-dimensional (3D) neural regeneration. (astm.org)
  • Overall, the study suggests that the composite structures could be used as a potential candidate for fabrication of scaffolds for neural regeneration with tunable electrical and mechanical properties by varying the CB concentration in a finite range. (astm.org)
  • To overcome these challenges, this thesis is focused on the development of a novel biomimetic tissue scaffold concept incorporating cell-mediated activation of growth factors for cartilage regeneration. (bl.uk)
  • This thesis demonstrates that the latent TGF-β1 biofunctionalised scaffolds induced chondrocytic differentiation in chondrocytes and more importantly the proof of concept of cell-mediated activation of growth factors as a novel approach for functional tissue regeneration. (bl.uk)
  • In this study biomaterial scaffolds for regeneration of nucleus pulposus were developed by freeze drying slurries with different proportions of collagen II (CII), chondroitin-6-sulfate (CS), and hyaluronic acid (HA). (mit.edu)
  • Matrix-enhancing molecules, such as TGF-β, are conjugated to or immobilized on scaffolds to increase ECM production by cells for tissue engineering, tissue regeneration and wound healing applications. (rice.edu)
  • Synthetic three-dimensional scaffolds for application to the regeneration of bone tissue should present an architecture similar to bone extracellular matrix and provide a suitable microenvironment for cell adhesion, proliferation and differentiation, ensuring tissue growth [1][2]. (scirp.org)
  • Mechanical strength of porous scaffolds are also crucial with respect to the regeneration of hard tissue such as bone, which must support a load and meet specific mechanical needs while stimulating bone regeneration. (scirp.org)
  • Such is the key to successful tendon/ligament tissue regeneration construct. (scientific.net)
  • The PLLA/HAp may interact with osteoblast and obtain desired effects that were stimulating osteoblast function and restraining the osteoclast function for bone tissue regeneration. (sigmaaldrich.com)
  • The fabrication method has a strong potential in the regenerative medicine field to produce customizable and defect-fillable scaffolds for tissue regeneration. (frontiersin.org)
  • Dr. Manitha B. Nair, Dr. Deepthy Menon, and Shantikumar V. Nair, "Porous Composite Fibrous Scaffold for Bone Tissue Regeneration", U.S. Patent 15/341,866 2016. (amrita.edu)
  • Bioactive three-dimensional (3D) scaffolds play a key role in the repair or regeneration of large bone defects. (rsc.org)
  • Our results suggest that the combination of 3D-plotting with the freeze-drying method is a viable strategy to construct hierarchical pore structures in 3D-plotted scaffolds, and the hierarchical pore structure plays an important role in improving the in vitro and in vivo osteogenesis of 3D-plotted bioceramic scaffolds for bone regeneration application. (rsc.org)
  • In this strategy, progenitors or mature cells are combined with biocompatible scaffolds to initiate partial or full bone and/or cartilage regeneration.Scaffolding materials in tissue engineering should be bioactive and biodegradable. (washington.edu)
  • Regeneration of damaged or malfunctioning tissues or organs is important goal of tissue engineering. (springer.com)
  • This chapter discusses about the importance of electrospun polymer fibers for regeneration of various tissues including bone, cartilage, heart muscles, liver and neural tissues. (springer.com)
  • They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses. (umassmed.edu)
  • adiponectin, VEGF-A, BMP-2 and DKK-1, with positive signals detected for adiponectin and VEGF-A. In order to develop an injectable, DAT-derived hydrogel for soft tissue regeneration, DATgels were fabricated from an enzymatically treated and homogenized DAT pre-gel suspension, which was neutralized to physiological pH and salt concentration. (queensu.ca)
  • For bone tissue engineering, human Adipose Derived Stem Cells (hADSCs) are proposed to be associated with a scaffold for promoting bone regeneration. (archives-ouvertes.fr)
  • The total market for the regeneration and repair of tissues and organs was estimated to be $25 billion worldwide in 2001 and is expected to rise steadily ( D&MD 2001 ). (royalsocietypublishing.org)
  • We are developing various types of materials, stimuli and processes so that we can have the means to achieve the regeneration of different tissue. (phys.org)
  • The overall goal of the current work was to develop scaffolds of native extracellular matrix components for soft tissue regeneration, more specifically, neural tissue engineering. (utexas.edu)
  • Regeneration of multi-tissue structures like the tendon-bone junction (TBJ) requires dynamic new biomaterial technologies. (nanohub.org)
  • Collagen-GAG (CG) scaffolds are FDA-approved biomaterials that have been applied to a variety of tissue regeneration challenges including skin, nerves, and cartilage. (nanohub.org)
  • Collagen-glycosaminoglycan (CG) scaffolds have previously been successfully employed in a variety of soft tissue regeneration applications. (nanohub.org)
  • His work is focused on the fabrication and characterization of multi-compartment collagen and gylcosaminoglycan scaffolds for tissue regeneration, with a particular interest in controlling the interface between two chemically and microstructurally different compartments. (nanohub.org)
  • Together with osteoconductive nanocomposite material and controlled growth factor delivery strategies, the use of SLS technique to form complex scaffolds will provide a promising route towards individualized bone tissue regeneration. (royalsocietypublishing.org)
  • The matrices or scaffolds serve as extracellular matrix to direct cell adhesion, proliferation and differentiation, and thus promote tissue regeneration. (royalsocietypublishing.org)
  • Intelligent scaffolds are designed to trigger the healing regeneration process through the controlled released or disposition of bioactive signals. (upc.edu)
  • These findings improve our understanding of how the mechanism scaffold microenvironmental cues guide bone tissue regeneration. (unl.edu)
  • Stevens MM. Biomaterials for bone tissue engineering. (springer.com)
  • State of the art and future directions of scaffold-based bone engineering from a biomaterials perspective. (springer.com)
  • The development of three-dimensional (3D) synthetic biomaterials as structural and bioactive scaffolds is central to fields ranging from cellular biophysics to regenerative medicine. (nih.gov)
  • As of yet, these scaffolds cannot electrically probe the physicochemical and biological microenvironments throughout their 3D and macroporous interior, although this capability could have a marked impact in both electronics and biomaterials. (nih.gov)
  • Here, we address this challenge using macroporous, flexible and free-standing nanowire nanoelectronic scaffolds (nanoES), and their hybrids with synthetic or natural biomaterials. (nih.gov)
  • NanoES exhibited robust electronic properties and have been used alone or combined with other biomaterials as biocompatible extracellular scaffolds for 3D culture of neurons, cardiomyocytes and smooth muscle cells. (nih.gov)
  • Compressed carbon dioxide (CO2) for decontamination of biomaterials and tissue scaffolds. (sc.edu)
  • Analysis of biomaterials used as scaffolds may, however, elucidate how TE can be enhanced. (mdpi.com)
  • Investigation of novel biomaterials for bone engineering represents an essential area for the design of tissue engineering strategies. (scielo.br)
  • In a paper in Biomaterials titled "Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold," (doi:10.1016/j.biomaterials.2010.10.056), UAB's Ho-Wook Jun and others in his group describe how they created their method to build a three-dimensional "cotton ball" of electrospun polycaprolactone that has the more desirable low density structure. (ceramics.org)
  • Scaffold biomaterials for tissue engineering can be produced in many different ways depending on the applications and the materials used. (royalsocietypublishing.org)
  • Instead, computer simulation applied to tissue engineering can offer a more exhaustive approach to test and screen out biomaterials. (royalsocietypublishing.org)
  • First we review the approach used in tissue engineering in the development of scaffolds and the interactions existing between biomaterials, cells and mechanical stimuli. (royalsocietypublishing.org)
  • Biomaterials development has evolved from first- and second-generation biomaterials, which sought primarily, respectively, biocompatibility and bioactivity with the surrounding tissues, to third-generation biomaterials, where biomaterials are designed to elicit a favourable and controlled bioactive response from the surrounding tissues ( Hench & Polak 2002 ). (royalsocietypublishing.org)
  • Within biomaterials, tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physico-chemical factors, to improve or replace biological functions. (royalsocietypublishing.org)
  • Overall, this chapter seeks to give an overview of the available biomaterials for use in combination with directed stem cell differentiation as means of replacing diseased or damaged tissues. (stembook.org)
  • Since 1987, the field has advanced considerably with recent work resulting in the development of implantable scaffolds that consist entirely of specific biomaterials. (stembook.org)
  • This review will focus on commonly used biomaterials that have been tested in combination with stem cells and the different applications for such scaffolds. (stembook.org)
  • Tissue engineering is a multidisciplinary field of research in which the cells, biomaterials, and processes can be optimized to develop a tissue substitute. (springer.com)
  • O'Brien, F.J.: Biomaterials & scaffolds for tissue engineering. (springer.com)
  • aims to bring together leading academic scientists, researchers and research scholars to exchange and share their experiences and research results on all aspects of Applying Polymeric Biomaterials in 3D Tissue Constructs and Scaffold. (waset.org)
  • Also, high quality research contributions describing original and unpublished results of conceptual, constructive, empirical, experimental, or theoretical work in all areas of Applying Polymeric Biomaterials in 3D Tissue Constructs and Scaffold are cordially invited for presentation at the conference. (waset.org)
  • ICAPBTCS 2021 has teamed up with the Special Journal Issue on Applying Polymeric Biomaterials in 3D Tissue Constructs and Scaffold . (waset.org)
  • Thus, this review provides an overview of the available biomaterials for directing stem cell differentiation as a means of producing replacements for diseased or damaged tissues. (iospress.com)
  • Since 1987, this field of study has expanded to include the design and development of implantable scaffolds with defined properties produced from a wide variety of biomaterials. (stembook.org)
  • In the current dissertation, we have developed such scaffolds of native biomaterials including hyaluronic acid (HA) and collagen. (utexas.edu)
  • This article reviewed advances in fabrication technology that enable the creation of biomaterials with well-defined pore structure and surface topography, which can be sensed by host tissue cells (esp. (unl.edu)
  • Scientific advances in biomaterials, stem cells, growth and differentiation factors, and biomimetic environments have created unique opportunities to fabricate or improve existing tissues in the laboratory from combinations of engineered extracellular matrices ("scaffolds"), cells, and biologically active molecules. (wikipedia.org)
  • Moreover, bioactivity and biocompatibility of the scaffolds were evaluated in vitro. (hindawi.com)
  • Likewise, the manner in which biomaterial scaffolds are 'resorbed' by osteoclasts in vitro and in vivo highlights more complex scenarios than predicted from knowledge of physiological bone resorption per se . (scielo.br)
  • The cell-loaded scaffolds were cultured in vitro for 5 days before implantation. (scientific.net)
  • Results revealed that nano-HA/PLGA composite scaffolds facilitated adheration of cells in vitro, and the nano-HA particles could prevented the scaffolds from collapsing and promoted the formation of cartilaginous tissue in vivo. (scientific.net)
  • These materials will be used next in In Vitro studies to determine their viability as regenerative tissue engineering constructs. (mit.edu)
  • The effect of the IL-4 boosted natural human host response on early tissue formation will be investigated in vitro. (tue.nl)
  • The fabrication of tubular structures is a promising approach to tissue engineering of vascular networks, which remains one of the major challenges in cultivation of thick tissues in-vitro. (jove.com)
  • The aim of this study is to prepare hierarchically porous composite scaffolds in order to improve the cell attachment, and further stimulate the in vitro and in vivo osteogenesis. (rsc.org)
  • The hybrid scaffolds demonstrated significantly-improved mechanical strength, water permeability and stability compared to pure chitosan scaffolds at increased porosity (92-95% vs. 87-89%).The in vitro study showed that the hybrid scaffold induced better cell attachment and proliferation rate, and maintained the functionality of the cells better than chitosan scaffolds. (washington.edu)
  • Scaffolds for tissue engineering prepared from biocompatible, biodegradable polymer-based, lighter than or light as water microcarriers and designed for cell culturing in vitro in a rotating bioreactor are provided. (freepatentsonline.com)
  • 2. The scaffold of claim 1 which is seeded with cells via culturing in vitro in a rotating bioreactor. (freepatentsonline.com)
  • and (c) seeding the scaffold with cells via culturing in vitro in a rotating bioreactor. (freepatentsonline.com)
  • In vitro assessment of Leu to hard tissue formation with coating on the well or addition in rBMC culture medium was also performed in a 6-well plate for 2 weeks. (scirp.org)
  • For example, autologous chondrocyte implantation (ACI) and matrix-induced ACI (MACI) are popular procedures that harvest and expand chondrocytes in vitro from the patient's own tissue, which are then grafted into the cartilage defect site with or without accompanying extracellular matrix (ECM). (frontiersin.org)
  • The in vitro fabrication of fully functional 3D vascular tissue construct represents one of the most fundamental challenges in vascular tissue engineering. (amrita.edu)
  • In vitro assessment of antibacterial activity and cytocompatibility of silver-containing PHBV nanofibrous scaffolds for tissue engineering. (biomedsearch.com)
  • The adhesion, viability, and proliferation properties of fibroblasts (NIH 3T3) and differentiation of osteoblasts (MC3T3-E1) were done to study in vitro cell compatibility of the scaffolds. (biomedsearch.com)
  • In previous in vitro and in vivo studies, decellularized adipose tissue (DAT) has demonstrated unique bioactivity, but little is known about the bioactive components preserved in the decellularized scaffold. (queensu.ca)
  • Preliminary in vitro cell work with adipose-derived stem cells (ASCs) showed that the 40 mg/mL formulation DATgel supported cell attachment and viability greater than 70% for all scaffolds up to 7 days after seeding. (queensu.ca)
  • Labelled cells were then seeded within a 3D porous polysaccharide-based scaffold and imaged in vitro using fluorescence microscopy and MRI. (archives-ouvertes.fr)
  • Traditionally, tissue engineering consists of harvesting cells from a patient, expanding them in vitro and culturing them into a biomaterial (also called a scaffold) that serves as a structural framework to allow cell attachment, proliferation and differentiation into a controlled phenotype ( Langer & Vacanti 1993 ). (royalsocietypublishing.org)
  • A relic process is used to produce bioresorbable ceramic scaffolds that can be used for in vitro or in vivo growth of human or animal tissue such as bone or cartilage. (patents.com)
  • The use of hybrid self-assembling peptide (EFK8)-carbon nanotube (SWNT) hydrogels for tissue engineering and in vitro 3D cancer spheroid formation is reported. (uwaterloo.ca)
  • Regarding tissue development in vitro, culture conditions such as pH, osmolality, temperature, nutrient, and metabolite concentrations dictate cell viability inside the constructs. (springer.com)
  • This work reviews the main culture and scaffold factors that affect tissue development in vitro regarding the culture of cells inside 3D matrices. (springer.com)
  • Combining stem cells with biomaterial scaffolds serves as a promising strategy for engineering tissues for both in vitro and in vivo applications. (iospress.com)
  • Few in vitro studies on PRF and no studies using human periosteal cells for tissue engineering have been published. (edu.au)
  • PRF membranes are suitable for in vitro cultivation of periosteal cells for bone tissue engineering. (edu.au)
  • Tissue engineering scaffolds are designed to support tissue self-healing within physiological environments by promoting the attachment, growth and differentiation of relevant cells. (springer.com)
  • Engineered replacements for musculoskeletal tissues generally require extensive ex vivo manipulation of stem cells to achieve controlled differentiation and phenotypic stability. (pnas.org)
  • The ability to control cell differentiation using cell-instructive scaffolds that have biomechanical properties approximating those of native tissue would represent a transformative advance in functional tissue engineering. (pnas.org)
  • The goal of this study was to develop a bioactive scaffold capable of mediating cell differentiation and formation of an extracellular matrix with the biochemical composition and mechanical features that mimic native tissue properties. (pnas.org)
  • Current approaches require extensive cell manipulation ex vivo, using exogenous growth factors to drive tissue-specific differentiation, matrix accumulation, and mechanical properties, thus limiting their potential clinical utility. (pnas.org)
  • The goal of this study was to generate a self-contained bioactive scaffold capable of mediating stem cell differentiation and formation of a cartilaginous extracellular matrix (ECM) using a lentivirus-based method. (pnas.org)
  • Chondrogenesis induced by scaffold-mediated gene delivery was as effective as traditional differentiation protocols involving medium supplementation with TGF-β3, as assessed by gene expression, biochemical, and biomechanical analyses. (pnas.org)
  • Rat neural stem cells (NSCs) were cultured in monolayer or in porous collagen scaffolds and exposed to neurogenic or non-neurogenic medium to determine the effects on neural differentiation and neurite growth. (mit.edu)
  • Tissue scaffolds are cell growth supporting structures composed of natural or synthetic biocompatible materials that provide a favorable environment for cell proliferation and differentiation. (marketresearch.com)
  • Efforts to induce vascular growth into tissue-engineered scaffolds have recently been dedicated to developing novel strategies to deliver specific biological factors that direct the recruitment of endothelial cell (EC) progenitors and their differentiation. (pnas.org)
  • Biomimetic scaffolds are therefore necessary to recapitulate this natural environment and provide various cues to direct cell processes and differentiation [ 4 , 5 ]. (omicsonline.org)
  • We successfully fabricated hierarchically porous bioceramic-silk (BC-silk) composite scaffolds by a combination of the 3D-plotting technique with the freeze-drying method, and further investigated the attachment, proliferation and osteogenic differentiation of bone marrow stromal cells (BMSCs) in the scaffolds as well as the in vivo osteogenesis of the prepared porous scaffolds. (rsc.org)
  • Among these techniques, scaffold assisted method is extensively employed as it acts as a supporting matrix for the cells, providing suitable microenvironment to facilitate the cell attachment, proliferation and differentiation. (springer.com)
  • It was concluded from the results that Leu might prevent the emigration of rBMCs to the outside of the scaffold and promote the differentiation of cells to osteoblasts in the scaffold. (scirp.org)
  • Scaffold construction with live stem cell incorporation and subsequent differentiation presents a promising route. (frontiersin.org)
  • This creates a need for more advanced studies that include aspects of mechanobiology in tissue engineering in order to be able to predict over time the growth and differentiation of tissues within scaffolds. (royalsocietypublishing.org)
  • Such biomaterial scaffolds can be used to promote the viability and differentiation of stem cells seeded inside-based on both the intrinsic properties of the material and the incorporation of specific cues into the material. (stembook.org)
  • It will also highlight studies that involve directed differentiation of stem cells into mature phenotypes as opposed to using biomaterial scaffolds for expansion of undifferentiated stem cells. (stembook.org)
  • These scaffolds are cultured with human mesenchymal stem cells (hMSCs) and support cell adhesion, growth, and chondrogenic differentiation. (illinois.edu)
  • The scaffolds support both hMSCs and mammary microvascular endothelial cells (MMECs) as well as osteogenic differentiation of the hMSCs. (illinois.edu)
  • Such biomaterial scaffolds promote the viability and differentiation of stem cells seeded inside depending on the intrinsic properties of the material as well as the incorporation of specific chemical and physical cues into the material. (stembook.org)
  • Finally, the biocompatibility of hMSCs within CG and CGCaP scaffold compartments was investigated using bioactivity, gene expression, and functional metrics as a precursor to both in vivo testing as well as subsequent work creating fully interpenetrating TBJ grafts that can drive multi-lineage MSC differentiation in a spatially-selective manner. (nanohub.org)
  • The surface-modified Ca-P/PHBV nanocomposite scaffolds loaded with rhBMP-2 significantly enhanced the alkaline phosphatase activity and osteogenic differentiation markers in gene expression of C3H10T1/2 mesenchymal stem cells. (royalsocietypublishing.org)
  • For cell-based tissue engineering, autologous or allogeneic cells, either determined or capable of differentiation, are first isolated. (royalsocietypublishing.org)
  • The scaffold should provide certain circumstance full of structural cues to enhance multipotent mesenchymal stem cell (MSC) differentiation, osteoblast growth, extracellular matrix (ECM) deposition, and subsequent new bone formation. (unl.edu)
  • Tissue engineering requires a mechanically stable, biocompatible, and biodegradable scaffold that permits cell adherence and proliferation, allows preservation of cell-specific properties, and suitable for surgical implantations. (nih.gov)
  • Our study proved that honeycomb collagen sheet is a mechanically stable, biocompatible and biodegradable scaffold for dermal tissue engineering, and also potentially useful for other cell-based therapies and tissue engineering applications. (nih.gov)
  • Self-assembled peptides gain increasing interest as biocompatible and biodegradable scaffolds for tissue engineering (1). (aiche.org)
  • Poly (L-lactic acid) (PLLA), a biodegradable and biocompatible polyester, has been used as material of for tissue engineered scaffold and regenerative medicine. (sigmaaldrich.com)
  • Plus, biocompatible materials, bioactive molecules, and cells can be printed together or separately to enhance scaffolds, which can save patients who suffer from shortage of transplantable organs. (frontiersin.org)
  • In vivo study showed that the hybrid scaffolds were biocompatible and degradable, and, promoted rapid vascularization, deposited connective tissue, and calcified matrix within the whole scaffold structure. (washington.edu)
  • With the goal of replacing damaged meniscus with engineered biologic tissue that can restore meniscal function, this work focuses on scaffolds composed of aligned biocompatible and biodegradable nanofibers that mimic the length-scale and organization of fibrous tissues. (upenn.edu)
  • 1. A scaffold for tissue engineering comprising biocompatible, biodegradable polymer-based, lighter than water or light as water microcarriers. (freepatentsonline.com)
  • We previously showed that implantation of porous scaffolds composed of a biodegradable and biocompatible block copolymer of Poly-lactic-co-glycolic acid and Poly-l-lysine improves functional recovery and reduces spinal cord tissue injury after spinal cord hemisection injury in rats. (mit.edu)
  • 1. A process for making biocompatible, bioresorbable ceramic implant device comprising a resorbable hard tissue scaffold, comprising: a) impregnating an organic fabric with at least one metal ceramic precursor and at least one phosphate ceramic precursor, b) heat treating the organic fabric to oxidize the fabric and form a biocompatible, bioresorbable ceramic green body, and c) sintering the biocompatible, bioresorbable ceramic green body, thereby forming said resorbable hard tissue scaffold. (patents.com)
  • 3D pHEMA scaffolds are patterned with varied architectures are rendered biocompatible by absorption of polylysine. (illinois.edu)
  • This research provides a foundation for future efforts based on biocompatible polymer and composite scaffolds in three-dimensional motifs. (illinois.edu)
  • The combination of biocompatible scaffolds formed from silk components, and stimulation of cells by means of magnetism is valid for generating bone tissue. (phys.org)
  • The material developed comprises a scaffold or matrix which in turn is made up of one of the main components of silk (fibroin), a biocompatible material of natural origin, and which is loaded with magnetic nanoparticles . (phys.org)
  • Cellulose and cellulose/recombinant type II collagen sponges were biocompatible for at least four weeks in cultivation, and gradual filling of the scaffold was observed. (iospress.com)
  • Abstract: A new method for the preparation of biodegradable porous scaffolds has been developed by using preprepared ice particulates as porogen material. (scientific.net)
  • Abstract: A novel porous composite scaffold of nano-HA/poly (lactic-co-glycolic) (PLGA) was fabricated by solvent casting/particulate leaching method. (scientific.net)
  • Abstract: Tissue defects and organ failure have seriously threatened the health and life of human beings and are challenges in modern medicine we have been trying to overcome. (scientific.net)
  • This paper addresses the computation of the effective diffusivity in new bioactive glass (BG) based tissue engineering scaffolds. (springer.com)
  • Bioactive glass and glass-ceramic scaffolds for bone tissue engineering. (springer.com)
  • This effect is achieved by controlled release of bioactive agents to the surrounding tissue. (springer.com)
  • Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. (springer.com)
  • Bioactive glass (BG) is widely used for bone tissue engineering. (hindawi.com)
  • This method opens new avenues in the development of bioactive implants that circumvent the need for ex vivo tissue generation by enabling the long-term goal of in situ tissue engineering. (pnas.org)
  • Tissue-engineering scaffolds were designed to mimic several features of the extracellular matrix using a combination of the synthetic polymer, PEG diacrylate, and bioactive factors. (rice.edu)
  • Scaffolds were formed by exposing aqueous solutions of PEG diacrylate and bioactive factors modified with PEG monoacrylate to ultraviolet or visible light in the presence of a suitable photoinitiator. (rice.edu)
  • Light exposure generated free radicals that targeted acrylate groups in the monomer and in PEG conjugated bioactive factors resulting in crosslinked hydrogel scaffolds with bioactive factors covalently incorporated. (rice.edu)
  • DeLong, Solitaire A.. "Bioactive scaffolds for optimizing engineered tissue formation. (rice.edu)
  • The ideal scaffold will also have bioactive characteristics, such as mechanical or chemical properties, to direct cells towards specific tissue phenotypes [ 5 ]. (omicsonline.org)
  • In this work a hybrid porous scaffold containing chitosan, polyvinyl alcohol and bioactive glass was successfully obtained and subsequently characterized by scanning electron microscopy. (scirp.org)
  • This paper provides a comprehensive overview of nanofibrous structures for tissue engineering purposes and the role of non-thermal plasma technology (NTP) within this field. (mdpi.com)
  • From thereon, the reader is introduced to the concept of NTP and its application in plasma-assisted surface modification of nanofibrous scaffolds. (mdpi.com)
  • Intervertebral disc tissue engineering using a novel hyaluronic acid-nanofibrous scaffold (HANFS) amalgam. (nih.gov)
  • In this study, we have seeded multipotent, adult human mesenchymal stem cells (MSCs) into a novel biomaterial amalgam to develop a biphasic construct that consisted of electrospun, biodegradable nanofibrous scaffold (NFS) enveloping a hyaluronic acid (HA) hydrogel center. (nih.gov)
  • The cartilaginous hyaluronic acid-nanofibrous scaffold (HANFS) construct architecturally resembled a native IVD, with an outer annulus fibrosus-like region and inner nucleus pulposus-like region. (nih.gov)
  • After isolation and expansion, MSCs are loaded onto the nanofibrous scaffold NFS ( A ) to ensure uniform cell distribution ( B ). A 250 μL aliquot of hyaluronic acid (HA)/MSC slurry is injected into the center of the NFS ( C ) to create the NP and tension the NFS layer to approximate the AF ( D ). (nih.gov)
  • Electrospinning has recently emerged as a widespread technology to produce synthetic nanofibrous and the best candidates for many important applications like scaffolds in tissue engineering. (igi-global.com)
  • Poly(L-lactic acid)/hydroxyapatite nanocylinders as nanofibrous structure for bone tissue engineering scaffolds. (sigmaaldrich.com)
  • To explore the use of nanofibrous scaffolds for engineering fibrocartilage, the effect of nanofiber alignment on the organization of cells and cell-deposited collagen was investigated. (upenn.edu)
  • Towards improving the functionality of aligned nanofibrous scaffolds, composites of multiple polymer fibers were investigated. (upenn.edu)
  • Additionally, the tissue forming potential of cells isolated from human sources was examined in a nanofibrous context. (upenn.edu)
  • Wang X, Ding B, Li B (2013) Biomimetic electrospun nanofibrous structures for tissue engineering. (springer.com)
  • Li W-J, Mauck RL, Cooper JA, Yuan X, Tuan RS (2007) Engineering controllable anisotropy in electrospun biodegradable nanofibrous scaffolds for musculoskeletal tissue engineering. (springer.com)
  • Morphological and chemical characterization of the nanofibrous scaffolds was carried out and the size of fibers ranged from 100 to 500nm. (amrita.edu)
  • Thus the results were suggestive of the fact that topographically aligned nanofibrous scaffolds control cellular organization and possibly provide a good support for achieving the vital organization and physical properties of blood vessel. (amrita.edu)
  • As the results, only silver-containing PHBV nanofibrous scaffolds showed a high antibacterial activity and an inhibitory effect on the growth of both Staphylococcus aureus and Klebsiella pneumoniae bacteria. (biomedsearch.com)
  • The work is described in the paper "Highly Aligned Nanofibrous Scaffold Derived from Decellularized Human Fibroblasts," coauthored by Zhao, postdoctoral researcher Qi Xing and undergraduate Caleb Vogt of MTU and Kam W. Leong of Duke University and published Jan. 29 in Advanced Funcational Materials . (kurzweilai.net)
  • Native tissues are endowed with a highly organized nanofibrous extracellular matrix (ECM) that directs cellular distribution and function. (kurzweilai.net)
  • The aligned nanofibrous ECM scaffold holds great potential in engineering organized tissues. (kurzweilai.net)
  • Such techniques have opened new areas of research in tissue engineering and regenerative medicine. (sciencemag.org)
  • The broad areas of tissue engineering and regenerative medicine seek to avoid some of these problems though the augmentation of the healing process or the fabrication of autologous tissue grafts that will not cause an immune response in the host. (sciencemag.org)
  • Although such a goal is unlikely to be attainable in the near future, printing and related technologies are of current use in the areas of tissue engineering and regenerative medicine. (sciencemag.org)
  • Regenerative medicine is a vastly growing field that aims to restore biological functions of damaged tissues or organs. (omicsonline.org)
  • Fitzpatrick, L.E., McDevitt, T.C.: Cell-derived matrices for tissue engineering and regenerative medicine applications. (springer.com)
  • In particular, the discovery of induced pluripotent stem cells (iPSCs) in 2006 catalyzed the fields of tissue engineering and regenerative medicine as they represented a major advance in stem cell technology [ 4 ]. (iospress.com)
  • The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues. (wikipedia.org)
  • Scaffolds based on natural marine sponges are characterised by lower oxygen diffusivity due to their lower porosity compared with the PU replica foams, which should enable the best oxygen supply to newly formed bone according the numerical results. (springer.com)
  • As a result, the oxygen diffusivity is determined by the scaffold porosity and pore architecture. (springer.com)
  • Scaffolds made by the FR method were found to have the highest oxygen diffusivity due to their high porosity and interconnected pores. (springer.com)
  • Karande TS, Ong JL, Agrawal CM. Diffusion in musculoskeletal tissue engineering scaffolds: design issues related to porosity, permeability, architecture, and nutrient mixing. (springer.com)
  • Creating porosity is the primary challenge of tissue engineering scaffolds. (igi-global.com)
  • Microspheres are being used to create scaffolds that allow controlled release of encapsulated matter (e.g. cells, growth factors, other phases) and with predetermined porosity depending on the size of the spheres. (europa.eu)
  • The produced hybrid scaffold was thoroughly characterized for mechanical and biological properties and compared to pure chitosan scaffolds of the same concentration and similar porosity. (washington.edu)
  • The diffusion coefficients of the composite scaffolds during the water absorption were much faster than those for the nonporous thin films, suggesting that the water uptake process depends on the presence of porosity and porous microstructure of the composite scaffolds. (hindawi.com)
  • One of the main characteristics that we look for in scaffolds and drug delivery systems is the formation of an adequate porosity. (upc.edu)
  • The role of this porosity is to ensure cell colonisation, flow transport of nutrients and metabolic waste in scaffolds for tissue engineering and to enhance the loading capacity and delivery rate in delivery systems. (upc.edu)
  • Characterization of the scaffolds included: microstructural evaluation by scanning electron microscopy, evaluation of the percentage of isolated porosity by helium pycnometry, rigidity test by mechanical testing and the study of the proportion of phases in the scaffold by X-ray diffraction analysis. (upc.edu)
  • Regarding the creation of scaffolds of inorganic matrix, the results, in terms of porosity and mechanical properties, were very promising and opened a new area of investigation. (upc.edu)
  • The scaffold made with glucose particles as porogen showed a narrower range of pore size with higher porosity and better inter-pore communications and seemed to encourage near normal cell morphology. (biomedsearch.com)
  • Three-dimensional (3D) architectural features from electrospun scaffolds, such as porosity, tortuosity, fiber diameter, pore size, and interconnectivity have a great impact on cell behavior. (springer.com)
  • Based on calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanocomposite microspheres, three-dimensional Ca-P/PHBV nanocomposite scaffolds with customized architecture, controlled porosity and totally interconnected porous structure were successfully fabricated using selective laser sintering (SLS), one of the rapid prototyping technologies. (royalsocietypublishing.org)
  • Importantly, multiple pore size distribution, interconnected pore structure and high porosity of scaffolds will facilitate vascularization and diffusion of nutrients and gases. (royalsocietypublishing.org)
  • Three important cues, including scaffold pore structure (i.e., porosity and pore size), grain size, and surface topography were studied. (unl.edu)
  • New manufacturing technologies under the banner of rapid prototyping enable the fabrication of structures close in architecture to biological tissue. (sciencemag.org)
  • However, the development of rapid prototyping techniques since the 1980s has enabled fabrication of fine-scale internal porous structures with the desired complexity, allowing a true engineering of the scaffold ( 1 , 2 ). (sciencemag.org)
  • An essential step in tissue scaffold fabrication is decellularization of the matrix. (sc.edu)
  • This collagen polymer supports custom fabrication of a broad range of collagen materials for various applications including tissue restoration, therapeutic cell and drug delivery, or enhancement of tissue-implantable devices interfaces. (purdue.edu)
  • Presented here is a simple-to-use, core/shell, three-dimensional bioprinting set-up for one-step fabrication of hollow scaffolds, suitable for tissue engineering of vascular and other tubular structures. (jove.com)
  • The main advantage of the core/shell technique is the simple production of hollow filament scaffolds in a single step, reducing the fabrication time and potentially allowing direct printing with cells. (jove.com)
  • A paper describing the results, "Low-Pressure Foaming: A Novel Method for the Fabrication of Porous Scaffolds for Tissue Engineering ," was featured in the February issue of the journal Tissue Engineering . (medicalxpress.com)
  • This review focuses on conducting polymer-based materials with biomimetic chemical, mechanical and topological properties, and recent progress toward the fabrication of clinically relevant tissue scaffolds is highlighted. (lancs.ac.uk)
  • Different strategies were tested in order to optimize the fabrication process of the scaffolds. (upc.edu)
  • Then, scaffold fabrication through CAD is presented and characterization of existing scaffolds through computed images is reviewed. (royalsocietypublishing.org)
  • In summary, direct-write assembly is a facile method for fabrication of 3D microperiodic scaffolds that model real tissues. (illinois.edu)
  • However, the developed scaffold fabrication procedure only enabled the achievement of the mechanical properties of the superficial zone, whereas the mechanical properties were too low to target the middle, deep and calcified zone. (epfl.ch)
  • This has signified a step forward in the line of research of this research group in the quest for suitable materials and methods for tissue fabrication. (phys.org)
  • Finally, we fabricated scaffolds of photopolymerizable HA with controlled microarchitecture and developed designer scaffolds for neural repair using layer-by-layer fabrication technique. (utexas.edu)
  • Recently strategies for creating mineralized CG (CGCaP) scaffold variants along with fabrication methods to create layered osteochondral scaffolds have been demonstrated. (nanohub.org)
  • Here I will discuss methods for the fabrication and characterization of both single and multi-compartment scaffolds targeted for the TBJ. (nanohub.org)
  • He has particularly enjoyed programming and applying his knowledge of material fabrication to tissue engineering. (nanohub.org)
  • Non-designed manufacturing techniques include most of the traditional scaffold fabrication methods, such as solvent casting/particulate leaching, phase separation, melt moulding and electrospinning. (royalsocietypublishing.org)
  • Puñet Ortiz, X. Design and fabrication of functionalized high porous poly(lactic acid)-based scaffolds for tissue engineering. (upc.edu)
  • The fabrication of intelligent scaffolds needs the development of novel techniques able to introduce biomimetic properties on the final product through easy and scalable techniques. (upc.edu)
  • This thesis is focused on the characterization and development of two versatile techniques for the fabrication of intelligent scaffolds. (upc.edu)
  • The second technique is based on the fabrication of highly porous poly(lactic) acid scaffolds with a nano-fibrous structure that resembles the natural extracellular matrix by means of a novel non-toxic, cheap and easy process. (upc.edu)
  • Human-like collagen (HLC) and nanohydroxyapatite (n-HAp) were used to fabricate the composite scaffold by way of cross-linking. (hindawi.com)
  • In this study, honeycomb collagen sheet was used for three-dimensional (3D) cultures of human skin fibroblasts and characterized as an effective and suitable scaffold for dermal tissue engineering. (nih.gov)
  • The cells attached quickly to the collagen scaffold, proliferated inside the honeycomb pores, and formed a structure similar to dermis within 60 days. (nih.gov)
  • Scanning electron microscopy of the 3D cultures showed a well-formed structure similar to dermis and biodegradation of the honeycomb collagen scaffold. (nih.gov)
  • Given the ubiquitous nature of the major collagen species in bone, collagen type I, the search for noncollagenous components endowed with the 'King Mida's touch' which would turn tissue into stone, focused mostly on noncollagenous components. (scielo.br)
  • Two kinds of novel biodegradable porous scaffolds for tissue engineering have been developed by our group by hybridizing synthetic poly(α-hydroxy acids) with naturally derived collagen. (scientific.net)
  • The synthetic polymer sponge, or mesh serving as a skeleton, reinforced the hybrid scaffolds and resulted in easy handling, while the collagen microsponges provided the hybrid sacffolds with a microporous structure and hydrophilicity, and therefore, easy cell seeding. (scientific.net)
  • A novel kind of hybrid biodegradable porous scaffold has been developed by forming collagen microsponges in the pores or interstices of a synthetic polymer sponge or mesh. (scientific.net)
  • A hybrid sponge of synthetic polymer, collagen and hydroxyapatite has been developed for hard tissue engineering. (scientific.net)
  • Bovine articular cartilage-like tissue has been engineered by culturing chondrocytes in the PLGA-collagen scaffolds. (scientific.net)
  • Scaffolds cultivated with fibroblasts for 3 weeks accumulated collagen (736 ± 193 μg/g wet weight) and DNA (17 ± 4 μg/g wet weight). (sigmaaldrich.com)
  • NSCs cultured in monolayer expressed more nestin and III-tubulin and had significantly longer neurite extensions than NSCs cultured in collagen scaffolds. (mit.edu)
  • Comparison of platelet rich fibrin and collagen as osteoblast-seeded scaffolds for bone tissue engineering applications. (nih.gov)
  • The aim of the present study is a comparison of platelet rich fibrin (PRF) with the commonly used collagen membrane BioGide(®) as a scaffold for human osteoblast cell seeding for bone tissue engineering. (nih.gov)
  • Although there are 28 known variations of this protein in the collagen superfamily, type I collagen is the most abundant in the human body and is commonly used for scaffold synthesis [ 10 ]. (omicsonline.org)
  • This is a first-of-a-kind, in situ scaffold-forming collagen. (purdue.edu)
  • This filler represents a highly purified liquid collagen protein, that when brought to physiologic conditions by mixing with a proprietary buffer, can be applied to tissue voids. (purdue.edu)
  • The liquid collagen conforms to patient-specific void geometries and then undergoes a self-assembly reaction to form a fibrillar collagen scaffold like those that make up the body's tissues. (purdue.edu)
  • This tissue filler represents the first planned medical product developed using our innovative collagen polymer technology," Harbin said. (purdue.edu)
  • In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering. (rsc.org)
  • Similarly to other soft collagenous tissues, toughening mechanisms in cornea are not well understood, but the lamellar structure of orthogonally aligned collagen fibrils in corneal stroma is thought to account for its toughness. (cam.ac.uk)
  • The hybrid scaffolds were also shown to promote the biomineralization of MG-63 osteoblast and the production of collagen type II, and maintain the characteristic cell morphology of chondrocytes. (washington.edu)
  • The highly ordered extracellular matrix of the meniscus is primarily composed of co-aligned collagen fibers, enabling the tissue to bear the high tensile loads seen with normal joint loading. (upenn.edu)
  • We have attempted to develop a tissue-engineered artery and heart valve based on the approach of entrapping tissue cells within a forming collagen gel. (umn.edu)
  • Adipose-derived aldehyde dehydrogenase-expressing cells promote dermal regenerative potential with collagen-glycosaminoglycan scaffold. (umassmed.edu)
  • The TGF-β3 group also produced a collagen type II and glycosaminoglycan-rich extracellular matrix, detected by immunohistochemistry, Alcian blue staining, and Safranin O staining suggesting robust chondrogenesis within the scaffold. (frontiersin.org)
  • The resulting RPE was cultured as a polarized monolayer on two different types of biodegradable scaffolds: a dual layer poly lacto-co-glycolic acid (PLGA) and a cross-linked electro-spun collagen scaffold. (arvojournals.org)
  • We compared cell viability on collagen and PLGA scaffolds and monitored the degradation of the scaffolds and cell growth over time. (arvojournals.org)
  • As compared to collagen, PLGA provides a better substrate for RPE scaffold and helps cells form a confluent monolayer. (arvojournals.org)
  • The aim is a comparison of PRF with the commonly used collagen membrane Bio-Gide® as scaffolds for periosteal tissue engineering. (edu.au)
  • Conclusion: PRF appears to be superior to collagen (Bio-Gide®) as a scaffold for human periosteal cell proliferation. (edu.au)
  • Technavio's analysts forecast the global tissue scaffolds market to grow at a CAGR of 12.14% during the period 2018-2022. (marketresearch.com)
  • The report covers the present scenario and the growth prospects of the global tissue scaffolds market for 2018-2022. (marketresearch.com)
  • Technavio's report, Global Tissue Scaffolds Market 2018-2022, has been prepared based on an in-depth market analysis with inputs from industry experts. (marketresearch.com)
  • There is an increasing trend of technological advances in the global tissue scaffolds market. (marketresearch.com)
  • Global Tissue Scaffolds Market 2018-2022, has been prepared based on an in-depth market analysis with inputs from industry experts. (sbwire.com)
  • Cheung ATM, Hu MS, Malhotra S, Peter Lorenz H, Longaker MT (2015) Biomimetic Scaffolds for Tissue Engineeringbiomimetic Scaffolds for Skin and Skeletal Tissue Engineering. (omicsonline.org)
  • Relative orientation of fibers in adjacent layers was found to significantly affect the overall fracture toughness and time-independent tensile behaviors of the fiber-reinforced hydrogels and is therefore a key to achieve tough biomimetic scaffolds for corneal tissue engineering. (cam.ac.uk)
  • Comparative characterization of 3-D hydroxyapatite scaffolds developed via replication of synthetic polymer foams and natural marine sponges. (springer.com)
  • The authors developed hybrid spools using the FDA-approved PCL polymer as the base material and mixing in poly-l-lactic acid or hydroxyapatite particles, and demonstrated the compatibility of these hybrid polymer spools with a readily accessible, commercial 3D printer to fabricate scaffolds capable of supporting the growth of live cells," says Editor-in-Chief Skylar Tibbits, Director, Self-Assembly Lab, MIT, and Founder & Principal, SJET LLC. (eurekalert.org)
  • Water uptake characteristics of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV-) based composite tissue engineering (TE) scaffolds incorporating nanosized hydroxyapatite (nHA) have been investigated. (hindawi.com)
  • Moreover, it was considered that a hybrid scaffold composed of a sponge and enveloped cylindrical porous hydroxyapatite (HA) would be convenient. (scirp.org)
  • Yabuuchi, T. , Yoshikawa, M. , Kakigi, H. and Hayashi, H. (2014) Hybrid Scaffolds Composed of Amino-Acid Coated Sponge and Hydroxyapatite for Hard Tissue Formation by Bone Marrow Cells. (scirp.org)
  • Supercritical carbon dioxide processing of poly--lactide (PLLA)/hydroxyapatite (nHA) nanocomposites was investigated as a means to prepare foams suitable as scaffolds in bone tissue engineering applications. (epfl.ch)
  • To produce 3D silk scaffolds at larger length scales for bone tissue engineering, hydroxyapatite (HA) nanoparticles are added to the silk fibroin inks to form a HA-filled silk ink. (illinois.edu)
  • An artist's impression of myoglobin-based artificial membrane binding proteins supplying oxygen to the stem cells during tissue engineering. (bristol.ac.uk)
  • Research led by the Universities of Bristol and Liverpool has shown that it is possible to combine cells with a special scaffold to produce living tissue in the laboratory. (bristol.ac.uk)
  • Until now, the approach has generally been limited to growing small pieces of tissue, as larger dimensions reduce the oxygen supply to the cells in the centre. (bristol.ac.uk)
  • By attaching an oxygen-carrying protein, myoglobin, to the stem cells before they are used to engineer cartilage, they ensure that each cell has its own oxygen reservoir that it can access when the oxygen in the scaffold drops to dangerously low levels. (bristol.ac.uk)
  • Professor Hollander's pioneering work includes the development of a method of creating cartilage cells from stem cells, which helped to make possible the first successful transplant of a tissue-engineered trachea, utilising the patient's own stem cells. (bristol.ac.uk)
  • Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue ' by J.P.K. Armstrong et al in Nature Communications [open access]. (bristol.ac.uk)
  • The ideal scaffold for use as a transplant not only has good biocompatibility, appropriate mechanical properties, and a well-matched degradation rate [ 3 - 5 ] but also has an appropriate pore size and high interconnectivity to promote cells attachment, proliferation, and bone repair [ 6 - 10 ]. (hindawi.com)
  • In their simplest form, these technologies allow the manufacture of scaffolds upon which cells can grow for later implantation into the body. (sciencemag.org)
  • Cells donated by the patient are expanded in culture and are then transferred to the scaffold. (sciencemag.org)
  • The scaffold provides a surface on which cells adhere, thrive, multiply, and generate the extracellular matrix (ECM) of structural and functional proteins and saccharides that make up living tissue. (sciencemag.org)
  • Both the scaffold material composition and its internal architecture (dimensions of the struts, walls, pores, or channels) control the behavior and well-being of the cells seeded inside. (sciencemag.org)
  • Similarly, within a tissue scaffold, all cells must be supplied with the means to maintain life, and this is achieved initially by providing a highly porous open structure to allow the uninterrupted flow and access of culture media in a bioreactor. (sciencemag.org)
  • The lack of efficient treatment modalities motivates researches into cartilage tissue engineering such as combing cells, scaffolds and growth factors. (ingentaconnect.com)
  • New Rochelle, NY, August 22, 2016--A new study describes the development of a novel hybrid polymer suitable for producing 3D-printed scaffolds on which living cells can be seeded to create engineered tissues. (eurekalert.org)
  • The authors incorporated living cells mixed with gelatin hydrogels into the scaffolds and achieved high levels of cell survival. (eurekalert.org)
  • Many aspects of the physiological process are recapitulated in systems of autologous or xenogeneic transplantation of osteogenic precursor cells developed for tissue engineering or modeling. (scielo.br)
  • The use of mineralized scaffolds for guiding bone tissue engineering has revealed unexpected manners in which the scaffold and cells interact with each other, so that a complex interplay of integration and disintegration of the scaffold ultimately results in efficient and desirable, although unpredictable, effects. (scielo.br)
  • The second part of the thesis evaluated the chondrogenic efficacy of the biofunctionalised scaffolds, using the aforementioned chondrocytes and human mesenchymal stem cells (MSC), in an in-vivo rat model. (bl.uk)
  • Three-dimensional biodegradable porous scaffolds play an important role in tissue engineering as temporary templates for transplanted cells to guide the formation of the new organs. (scientific.net)
  • The hybrid scaffolds were used for three-dimensional culture of fibroblast, tenocytes, chondrocytes and mesenchymal stem cells for tissue engineering of skin, ligament, cartilage and osteochondral tissue. (scientific.net)
  • These cells adhered and spread well in the hybrid scaffolds, proliferated, secreted extracellular matrices and formed the respective tissues. (scientific.net)
  • Laminin coated scaffolds promoted the attachment of NSCs to the scaffold struts and resulted in a more even distribution of nestin and [beta]III-tubulin positive cells throughout the scaffold. (mit.edu)
  • The essential precondition for new tissue generation is an extracellular matrix which acts as a scaffold so that cells can migrate, differentiate, and proliferate. (nih.gov)
  • 7. The biomaterial scaffold of claim 1, wherein the reconstituted extracellular matrix is derived from cultured cells or animal tissue. (freepatentsonline.com)
  • 15. The biomaterial scaffold of claim 1, further comprising at least one biologically active agent, and wherein the biologically active agent comprises a plurality of cells seeded within the polyelectrolyte complex fibers. (freepatentsonline.com)
  • 21. A biomaterial scaffold comprising reconstituted extracellular matrix, polyelectrolyte complex fibers and seeded cells, wherein the extracellular matrix is derived from the same or similar cell type as the seeded cells. (freepatentsonline.com)
  • A novel polymer-surfactant complex of oxygen-carrying protein myoglobin is delivered to the cytoplasmic membrane of stem cells, which are then used to engineer cartilage constructs with significantly enhanced oxygen distribution to the center of the tissue (credit: James P.K. Armstrong et al. (kurzweilai.net)
  • Currently, tissue engineering has been limited to growing small pieces of tissue, because larger dimensions reduce the oxygen supply to the cells in the center of the tissue. (kurzweilai.net)
  • Additionally, PEG hydrogels were modified with a degradable peptide sequence to enable cells to remodel the scaffold by secreting matrix metalloproteinases (MMPs). (rice.edu)
  • Cells were observed to change their morphology and migrate when seeded within these degradable hydrogel scaffolds, but not in scaffolds lacking this degradable peptide sequence. (rice.edu)
  • The matrix-enhancing molecule retains activity after attachment to the scaffold, and causes cells growing in or on the scaffold to increase extracellular matrix (ECM) production, without substantially increasing proliferation of the cells, even when the scaffold additionally contains cell adhesion ligands. (rice.edu)
  • The increased ECM produced by the cells aids in maintaining the integrity of the scaffold, particularly when the scaffold is degradable, either by hydrolysis or by enzymatic degradation. (rice.edu)
  • In this approach, the neotissue can be constructed by using a combination of specific cells, growth factors, and 3D porous scaffolds alone or in combination ( 3 ). (pnas.org)
  • The primary function of vasculature is to facilitate the transport of nutrients and oxygen to cells invading from the host tissue and cells transplanted in the scaffolds. (pnas.org)
  • In situ tissue engineering (TE) using a biodegradable synthetic scaffold that recruits endogenous cells from the bloodstream is emerging as a promising technology to create living heart valves inside the human body having the potential to last a life-time. (tue.nl)
  • Monocytes differentiate into macrophages, which in turn attract progenitor cells that differentiate into tissue-producing cells. (tue.nl)
  • By seeding cells on cadaveric hearts stripped down to their underlying structure, a team of researchers led by Andrew Wan and Karthikeyan Narayanan at the A*STAR Institute of Bioengineering and Nanotechnology (IBN) have produced two different types of heart tissues, both of which will be needed to make a fully beating organ. (nextbigfuture.com)
  • The researchers set out with a simple question: could the scaffold left behind in so-called 'decellularized' hearts guide pluripotent cells to form new heart tissues without the need for external cues? (nextbigfuture.com)
  • Next, the researchers repopulated the scaffold with either human embryonic stem cells (ESCs), which can change into all cell types of the human body, or mesendodermal cells (MECs), which can give rise to heart cells only. (nextbigfuture.com)
  • Independent analyses of stem cell-derived heart muscle tissues have shown that the more mature cells tend to favor the expression of the light chain over the heavy chain. (nextbigfuture.com)
  • Although the IBN team did not observe any beating cells in their scaffold, they believe that MECs alone would be the better choice as they are the more committed cell type. (nextbigfuture.com)
  • In addition to forming heart muscle cells, the seeded stem and progenitor cells also gave rise to endothelial cells, another crucial type of heart tissue, with the differentiated cell type ultimately dependent on where the implanted cells came into contact with the scaffold. (nextbigfuture.com)
  • Load the cells into a sterile 5 mL syringe equipped with a 27 gauge needle and locate an entry point in the scaffold. (jove.com)
  • Scaffolds artificial, lattice-like structures capable of supporting tissue formation are necessary in this process to provide a template to support the growing cells. (medicalxpress.com)
  • Scaffolds are typically engineered with pores that allow the cells to migrate throughout the material. (medicalxpress.com)
  • Various techniques such as cell sheet engineering, cell spheroids, scaffold assisted methods and 3D printing of the cells with polymers have been tested in tissue engineering. (springer.com)
  • 5. A method for regenerating a selected tissue comprising seeding the scaffold of claim 1 with cells which generate the selected tissue and culturing the scaffold and seeded cells in a rotating bioreactor. (freepatentsonline.com)
  • Numerous unphosphorylated neurofilament H and neuronal growth associated protein positive fibers and myelin basic protein positive cells may indicate neural sprouting inside the remodeled tissue layer of treated monkeys. (mit.edu)
  • Embryonically inspired scaffolds regulate tenogenically differentiating cells. (umassmed.edu)
  • Influence of scaffold properties on the inter-relationship between human bone marrow derived stromal cells and endothelial cells in pro-osteogenic conditions. (umassmed.edu)
  • Stem cells placed on the scaffold thrived, and it had the added advantage of provoking a very low immune response. (kurzweilai.net)
  • Feng Zhao of Michigan Technological University (MTU) has persuaded fibroblasts - cells that make up the extracellular matrix in the body - to make a well-organized nanopatterned scaffold (support structure). (kurzweilai.net)
  • And, since her scaffold is made by cells, it is composed of the same intricate mix of all-natural proteins and sugars found in the body. (kurzweilai.net)
  • The trick was to orient the cells on a nano-grate (130 nm in depth) that guided their growth and the creation of the scaffold. (kurzweilai.net)
  • Stem cells placed on her scaffold thrived, and it had the added advantage of provoking a very low immune response. (kurzweilai.net)
  • Reseeding human mesenchymal stem cells (hMSCs) shows the excellent capacity of the scaffold in directing and supporting cell alignment and proliferation along the underlying fibers. (kurzweilai.net)
  • Organs are made by plac- ing a culture of cells from a patient's organ onto a shaped tissue scaffold. (sciencephoto.com)
  • Fed by special nutrients the cells multiply using the fibres as a scaffold to form an organ in a few weeks. (sciencephoto.com)
  • Forty million renal cortical tubular epithelial (RCTE) cells are injected through the renal artery, and rapidly perfused through the scaffold under high flow (25 ml/min) and pressure (~230 mmHg) for 15 min before reducing the flow to a physiological rate (4 ml/min). (jove.com)
  • Building functioning kidney tissue from a patient's own cells would eliminate the need for lifelong immunosuppression, decrease the amount of time patients spend on dialysis waiting for a kidney transplant, and extend life-saving transplantation to more patients with chronic kidney disease. (jove.com)
  • In a UAB news release, Bryan A. Blakeney, a recent graduate student and one of the authors is quoted as saying, "Our three-dimensional electrospun nanoscaffolds better mimic nature and encourage cells to live longer and generate more viable, or functional, tissues. (ceramics.org)
  • We've separated the grains of sand to create gaps, and the cells fill and penetrate the gaps between the sand grains to form tissues. (ceramics.org)
  • In the paper, the group says they tested the performance of the FLUF ePCL material and report, "Cells seeded on the cotton ball-like scaffold infiltrated into the scaffold after seven days of growth, compared to no penetrating growth for the traditional electrospun scaffold. (ceramics.org)
  • Quantitative analysis showed approximately a 40 percent higher growth rate for cells on the cotton ball-like scaffold over a seven-day period, possibly due to the increased space for in-growth within the three-dimensional scaffolds. (ceramics.org)
  • Magnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering. (archives-ouvertes.fr)
  • These data suggested that this scaffold might behave as a cell carrier capable of both holding a cell fraction and delivering cells to the site of implantation. (archives-ouvertes.fr)
  • Several case studies of finite-element studies in tissue engineering show the usefulness of computer simulations in determining the mechanical environment of cells when seeded into a scaffold and the proper design of the geometry and stiffness of the scaffold. (royalsocietypublishing.org)
  • These committed cells can form an extracellular matrix to produce the desired tissue within the biomaterial scaffold. (royalsocietypublishing.org)
  • Combining stem cells with biomaterial scaffolds provides a promising strategy for engineering tissues and cellular delivery. (stembook.org)
  • This review seeks to describe the current types of scaffolds and evaluate their use in combination with stem cells for tissue engineering applications. (stembook.org)
  • Finally, conclusions about the current state of biomaterial scaffolds containing stem cells for tissue engineering applications are drawn and suggestions for the future direction of the field are given. (stembook.org)
  • This review will focus mainly on three-dimensional (3D) scaffolds as they are the most appropriate for developing engineered tissues and delivering cells for in vivo applications. (stembook.org)
  • Previous work has shown that transplanting RPE cells on a scaffold rather than cell suspension provides greater viability and integration into the host retina. (arvojournals.org)
  • In addition, the idea would be to use the stem cells of the patients themselves and be capable of differentiating them towards the type of cell we want to form the tissue with, be it bone, muscle, heart or whatever might be needed. (phys.org)
  • This updated review details commonly used biomaterial scaffolds for engineering tissues from stem cells. (iospress.com)
  • We first define the different types of stem cells and their relevant properties and commonly used scaffold formulations. (iospress.com)
  • Tissue engineering combines biomaterial scaffolds with these different types of cells to replace damaged organs. (iospress.com)
  • Cells and/or biomolecules are encapsulated within the matrices or seeded onto the surface of three-dimensional scaffolds. (royalsocietypublishing.org)
  • Tissue cells reside in a complex scaffold physiological microenvironment. (unl.edu)
  • The following are among the main causes of these failures: studying molecular mechanisms of cancer development, identifying therapeutic targets, and testing drug candidates using inappropriate tissue culture models, which do not recapitulate the native microenvironment where the cancer cells originate. (sciencemag.org)
  • Cancer cells in human tissues have contacts with the extracellular matrix (ECM) in all directions and interact with other cells of the same (or different) type in their vicinity. (sciencemag.org)
  • Tissue engineering is a biomedical engineering discipline that uses a combination of cells, engineering, materials methods, and suitable biochemical and physicochemical factors to restore, maintain, improve, or replace different types of biological tissues. (wikipedia.org)
  • Tissue engineering often involves the use of cells placed on tissue scaffolds in the formation of new viable tissue for a medical purpose but is not limited to applications involving cells and tissue scaffolds. (wikipedia.org)
  • He proposed the joining of the terms tissue (in reference to the fundamental relationship between cells and organs) and engineering (in reference to the field of modification of said tissues). (wikipedia.org)
  • In addition, the cross-linking technique can increase the mechanical properties of the scaffolds. (hindawi.com)
  • The results showed that HANw not only improved the mechanical properties but also enhanced bioactivity of the scaffolds. (hindawi.com)
  • The influence of HANw on the microstructure and mechanical properties of 63s glass scaffolds were investigated. (hindawi.com)
  • The introduction of 3D printing or additive manufacturing is increasingly being adopted for manufacturing scaffolds as it allows the researcher to control the size, shape, pore size, geometry, and mechanical properties of the scaffold. (marketresearch.com)
  • Bombyx mori silk, though used in biomedical sutures for decades due to its excellent mechanical properties, has been overlooked for applications in ligament tissue engineering, only until recently. (scientific.net)
  • There are many methods to prepare 3D scaffolds, among which the 3D-plotting technique is a promising strategy as the scaffolds prepared by this method possess not only improved mechanical properties and interconnectivity, but also ordered large-pore structure. (rsc.org)
  • The prepared BC-silk composite scaffolds possessed excellent apatite-mineralization ability and mechanical properties with compressive strength up to 25 MPa. (rsc.org)
  • The mechanical properties of the foams were nevertheless comparable with those of trabecular bone, and by adjusting the saturation pressure and depressurization rate it was possible to generate porosities of about 85 %, an interconnected morphology and cell diameters in the range 200-400 mu m from PLLA containing 4.17 vol% nHA, satisfying established geometrical requirements for bone replacement scaffolds. (epfl.ch)
  • By comparing the ink viscoelasticity to that of the cured scaffolds, we find that the chemical gel provides the dominant contribution to the mechanical properties. (illinois.edu)
  • The mechanical properties of the 3D silk scaffolds are probed by atomic force microscopy nanoindentation. (illinois.edu)
  • Recently, it was hypothesized that one possibility to engineer pure hyaline cartilage is the production of scaffolds which mimic the mechanical properties and zonal structure of native cartilage. (epfl.ch)
  • The overall goal with my Ph.D. project is the development of a scaffold based on decellularised articular cartilage, which has zone-specific mechanical properties to induce zonal lineage commitment in chondro-progenitors. (epfl.ch)
  • In the first section, the zonal mechanical properties of human articular cartilage were measured by instrumented indentation which was information crucial to targeting the appropriate properties in scaffolds. (epfl.ch)
  • It was demonstrated that the mechanical properties of these scaffolds could be tailored by changing the digest concentration prior to lyophilization. (epfl.ch)
  • This confirms that mimicking the zone-specific mechanical properties in these prepared scaffolds can produce zonal lineage commitment. (epfl.ch)
  • biocompatibility, mechanical properties similar to the target tissue, biodegradability at a rate commensurate with tissue formation rate, etc. (royalsocietypublishing.org)
  • They will also affect the mechanical properties and degradation rate of the scaffolds. (royalsocietypublishing.org)
  • Chemically cross-linked scaffolds were analyzed to determine pore size and cross-link density. (mit.edu)
  • The addition of CS to the scaffold increased pore size. (mit.edu)
  • The scaffold presented satisfactory pore size range and open interconnected pores, which are essential for tissue ingrowth. (scirp.org)
  • Among other properties, these scaffolds should exhibit biocompatibility with the damaged tissue, interconnected pore network, pore size ranging from 100 to 300 µm, mechanical strength similar to bone tissue, and biodegradability at the rate at which tissue regenerates [3][4]. (scirp.org)
  • Sintering results in fibers of the fabric being cross-sintered with one another to form a three-dimensional scaffold structure having controlled pore size and distribution. (patents.com)
  • Characterization of a Novel Polymeric Scaffold for Potential Application in Tendon/Ligament Tissue Engineering. (scientific.net)
  • Bulk mechanical and permeability characterization methods were applied and then compared with ultrasound elastography approaches targeting non-destructive analysis of individual scaffold compartments within the heterogeneous multi-compartment scaffolds. (nanohub.org)
  • Then we present examples of selected material types (natural polymers and synthetic polymers) and fabricated forms of the scaffolds (three-dimensional scaffolds, micro- or nanoparticles, and their composites). (ingentaconnect.com)
  • Data will be used to allow the development of an IL-4 loaded synthetic scaffold that selectively activates the wound healing M2 phenotype. (tue.nl)
  • The performance of biopolymer and synthetic degradable polymer based electrospun fibers in tissue engineering will also be briefly reported. (springer.com)
  • Okamoto M, John B (2013) Synthetic biopolymer nanocomposites for tissue engineering scaffolds. (springer.com)
  • Tissue engineering has shown great potential in the treatment and understanding of a large number of degenerative ailments, including osteoarthritis and cardiovascular disease, through the design of biological scaffolds using both synthetic biodegradable polyesters and naturally occurring extracellular matrix materials. (findaphd.com)
  • Typically, researchers construct scaffolds from synthetic materials or natural animal or human substances grown in a Petri dish. (kurzweilai.net)
  • KurzweilAI has reported a variety of such synthetic approaches to creating scaffolds for extracellular matrices. (kurzweilai.net)
  • Potential scaffold materials are classified as natural or synthetic and the advantages and drawbacks of each material are detailed. (stembook.org)
  • Next, we discuss natural and synthetic scaffold materials typically used when engineering tissues, along with their associated advantages and drawbacks and gives examples of target applications. (iospress.com)
  • Scaffolds exist in hydrogel or porous forms and are made from either natural materials or synthetic polymers, as previously described ( 1 , 9 , 10 ). (sciencemag.org)
  • In all cases, oxygen diffusion is confined to the scaffold pores that are assumed to be completely occupied by newly formed tissue. (springer.com)
  • In one aspect, the invention provides a porous metal scaffold comprising a porous metal network having pores defined by metal webs, the metal webs covered with at least one layer of metal particles. (google.com)
  • The results showed that the hierarchical structure in the composite scaffolds was composed of first-level pores (∼1 mm) of the bioceramic scaffold and second-level pores (∼50-100 μm) of the silk matrix. (rsc.org)
  • Leu-coated PVF sponge in the scaffolds showed marked new bone formation in the pores by histological examination. (scirp.org)
  • The structure network has been improved in the case of scaffolds with organic matrix, by decreasing the content of closed pores and also by ensuring total incorporation of the α-TCP powder in the scaffold. (upc.edu)
  • Fluorescence staining, the Cell Counting Kit-8 (CCK-8) assay, and scanning electron microscopy (SEM) indicated that the scaffolds enhanced cell adhesion and proliferation. (hindawi.com)
  • The potential of the developed composite microcylinders could be a promising way to fabricate the microfibrous structural scaffold that accelerates cell proliferation and functions for bone tissue engineering. (sigmaaldrich.com)
  • BC-silk scaffolds with hierarchical pore structures showed distinctively improved cell proliferation, ALP activity and bone-related gene expression as compared to BC scaffolds without hierarchical pore structure. (rsc.org)
  • Cardiomyocytes cultured on a PMNT scaffold triggered proliferation and significantly increased the expression of cardiac-specific gap junction protein, namely connexin 43. (diva-portal.org)
  • 3D macroporous nanoES mimic the structure of natural tissue scaffolds, and they were formed by self-organization of coplanar reticular networks with built-in strain and by manipulation of 2D mesh matrices. (nih.gov)
  • There is also an increasing focus on the development of scaffolds containing nanomaterials as they can mimic the architecture of the normal extracellular matrix containing both nanofibers and microfibers. (marketresearch.com)
  • But such scaffolds have not been able to mimic the highly organized structure of the matrix made by living things - at least until now. (kurzweilai.net)
  • Such scaffolds are necessary to accurately mimic the complex environment of real tissues. (illinois.edu)
  • This review focuses on three-dimensional (3D) scaffolds as they mimic the environment found in the human body, making them highly relevant when engineering tissues, including the use of 3D printing tissues using biomaterial-based inks. (stembook.org)
  • Drury JL, Mooney DJ (2003) Hydrogels for tissue engineering: scaffold design variables and applications. (springer.com)
  • Statement of significance For the first time we used hybrid self-assembling peptide-carbon nanotube hybrid hydrogels (that we have recently introduced briefly in the "Carbon" journal in 2014) for tissue engineering and 3D tumor engineering. (uwaterloo.ca)
  • We showed the potential of these hybrid hydrogels to enhance the efficiency of the peptide hydrogels for tissue engineering application in terms of cell behavior (cell attachment, spreading and migration). (uwaterloo.ca)
  • Also our system (peptide and peptide-CNT hydrogels) was used for cancer cell spheroid formation showing the effect of both tumor microenvironment stiffness and cell-scaffold adhesion on cancer cell invasion. (uwaterloo.ca)
  • However, the lack of the necessary tissue ECM components limits the applications of these types of hydrogels in studies of mammalian cell biology and compromises the reliability of the related data for the interpretation of human pathophysiological conditions. (sciencemag.org)
  • Furthermore, we will present how resveratrol scaffolds affect adipose tissue remodeling that could play a role in fatty acid utilization. (aiche.org)
  • The regenerative tissue filler, when applied to breast tissue voids, such as those associated with breast conserving surgery, restored breast shape and consistency and supported new breast tissue formation over time, including mammary glands, ducts and adipose tissue. (purdue.edu)
  • hADSCs were isolated from adipose tissue and labelled with USPIO-rhodamine (Ultrasmall SuperParamagnetic Iron Oxide). (archives-ouvertes.fr)
  • In this study, we developed conductive composite hydrogel films based on gelatin and carbon black (CB) as scaffolds for neural tissue engineering applications. (astm.org)
  • This hydrogel system is expected to be useful for studying tissue formation leading eventually to an improved understanding of the factors needed to form engineered tissues. (rice.edu)
  • Preparing a hydrogel formulation with the appropriate viscoelastic properties, and sustaining a continuous balanced flow of the core/shell materials is crucial for 3D printing of stable scaffolds. (jove.com)
  • Interconnected channels between sponge skeleton and hydrogel may further aid in tissue integration and nutrient perfusion. (arvojournals.org)
  • Decellularization is currently done by contacting xenographic tissue with a combination of chemical detergents and biological agents. (sc.edu)
  • Biological effects of the biofunctionalised scaffold were assessed using human nasal chondrocytes in a serum free environment and compared with conventional TGF-β1 supplementation on non-biofunctionalised scaffolds (as control). (bl.uk)
  • Taken together, these findings suggest the prototypic potential of MSC-seeded HANFS constructs for the tissue engineering of biological replacements of degenerated IVD. (nih.gov)
  • The scaffolds can also incorporate nano-sized fibers, providing a new range of mechanical and biological properties, Ameer said. (medicalxpress.com)
  • Herath, H.M., Di Silvio, L. and Evans, J.R. (2010) Biological Evaluation of Solid Free Formed, Hard Tissue Scaffolds for Orthopedic Applications. (scirp.org)
  • Highly aligned nanofibers created by fibroblasts form a biological scaffold that could prove an ideal foundation for engineered tissues. (kurzweilai.net)
  • However, they are not considered ideal models for cancer studies because of the inconsistencies in their formation [which varies with cell types ( 6 )], the challenges encountered in handling them, the absence of tissue ECM components, and their controversial biological relevance ( 8 ). (sciencemag.org)
  • A non-woven fibrous scaffold was fabricated by electrospinning, surface modified using ammonia plasma, followed by scaffold surface biofunctionalisation with the latent TGF-β1. (bl.uk)
  • Cartilaginous tissues were observed at defects repaired with the cell-loaded scaffolds, while only fibrous tissues were found for the control groups. (scientific.net)
  • This work represents a significant advance in meniscus tissue engineering, and has bearing on other fibrous, anisotropic tissues such as the temporomandibular fibrocartilage, annulus fibrosus of the intervertebral disc, as well as tendon and ligament. (upenn.edu)
  • Cheng L, Sun X, Zhao X, Wang L, Yu J, Pan G et al (2016) Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars. (springer.com)
  • The objective of this study is to create a purely natural, uniform, and highly aligned nano-fibrous ECM scaffold for potential tissue engineering applications. (kurzweilai.net)
  • Effect of scaffold architecture on diffusion of oxygen in tissue engineering constructs. (springer.com)
  • Furthermore, we show the integrated sensory capability of the nanoES by real-time monitoring of the local electrical activity within 3D nanoES/cardiomyocyte constructs, the response of 3D-nanoES-based neural and cardiac tissue models to drugs, and distinct pH changes inside and outside tubular vascular smooth muscle constructs. (nih.gov)
  • Cell-scaffold constructs were implanted into subcutaneous pockets of athymic rats for six weeks. (bl.uk)
  • Restricted oxygen diffusion can result in central cell necrosis in engineered tissue, a problem that is exacerbated when engineering large tissue constructs for clinical application. (kurzweilai.net)
  • Attempts to incorporate engineered three-dimensional liver tissue in bioartificial liver devices or in implantable tissue constructs, to treat or bridge patients to self-recovery, were met with many challenges, amongst which is to find suitable polymeric matrices. (biomedsearch.com)
  • The mathematical modeling of the relationship between these factors and cell behavior inside 3D constructs has also been critically reviewed, focusing on mesenchymal stem cell culture in electrospun scaffolds. (springer.com)
  • We investigated the feasibility of oleuropein as a cross-linking agent for fabricating three-dimensional (3D) porous composite scaffolds for bone tissue engineering. (hindawi.com)
  • Modelling oxygen diffusion and cell growth in a porous, vascularizing scaffold for soft tissue engineering applications. (springer.com)
  • Analysis of cell growth and diffusion in a scaffold for cartilage tissue engineering. (springer.com)
  • Tissue engineering merges the disciplines of study like cell biology, materials science, engineering and surgery to enable growth of new living tissues on scaffolding constructed from implanted polymeric materials. (unt.edu)
  • The biofunctionalised scaffold group induced a lower cell metabolic activity and significantly higher gene expression of cartilage specific transcription factor Sox9 after 14 days. (bl.uk)
  • Full-thickness articular cartilage defects were created in rabbits, and filled with and without the cell-loaded nano-HA/PLGA scaffolds. (scientific.net)
  • 11. The biomaterial scaffold of claim 1, wherein the reconstituted extracellular matrix is derived from an osteoblast cell line or a hepatocarcinoma cell line. (freepatentsonline.com)
  • 12. The biomaterial scaffold of claim 10, wherein the osteoblast cell line is MC-3T3. (freepatentsonline.com)
  • 13. The biomaterial scaffold of claim 10, wherein hepatocarcinoma cell line is HepG2. (freepatentsonline.com)
  • The functionalization technology is facile, versatile and non-disruptive, and in addition to tissue oxygenation, it should have far-reaching application in a host of tissue engineering and cell-based therapies. (kurzweilai.net)
  • The matrix-enhancing molecule is conjugated to a tether, such as polyethylene glycol (PEG) monoacrylate, for attachment to a tissue engineering or cell growth scaffold. (rice.edu)
  • Rather, it can be considered as a natural agent of tissue remodeling, orchestrated by various cell types and potent signaling molecules. (tue.nl)
  • Mimicking the 3D structure and extracellular matrix (ECM) of native tissue is critical for successful cell transplantation and growth of artificial tissue [ 3 ]. (omicsonline.org)
  • However, there were some important differences between the tissues depending on the cell source. (nextbigfuture.com)
  • Then place the scaffold into a colorless cell culture medium and incubate the scaffold at 37 degrees Celsius with 5%carbon dioxide for at least 24 hours. (jove.com)
  • Confirm that the needle is within the hollow filament of the channel and gently depress the plunger to inject approximately 1-2 mL of cell suspension into the scaffold. (jove.com)
  • When the entire scaffold has been filled with cell suspension, submerge the scaffold in fresh cell culture medium, and return the scaffold to the cell culture incubator for up to 10 days. (jove.com)
  • With the ability to generate organized collagenous tissues via nanofiber alignment, the remainder of this work focused on optimizing the combination of scaffold, relevant cell type, and inducers of tissue formation. (upenn.edu)
  • Using a multi-pronged approach, the formation of fibrocartilage was optimized through scaffold design, cell source selection, and modulation of the mechanical environment. (upenn.edu)
  • From Asymmetric stem cell division to tissue engineering. (cam.ac.uk)
  • NanobioMatrix Scaffolds are novel electrospun scaffolds made with nano-scale polymer fibers for applications in tissue engineering, stem cell research, cancer research and three-dimensional culture. (reprap.org)
  • NanobioMatrix scafflolds are similar in size and structure to native extracellular matrix (ECM) and mimics the three-dimensional in vivo environment to provide a superior alternative to traditional cell culture on flat, tissue culture polystyrene (TCPS) plates or inside bioreactors. (reprap.org)
  • These protocols can be used to recellularize kidney scaffolds with a variety of cell types, including vascular endothelial, tubular epithelial, and stromal fibroblasts, for rapid evaluation within this system. (jove.com)
  • The problem the group was trying to solve when they made their discovery was that the typical electrospun mat doesn't do so well serving as a framework for facilitating cell and tissue growth. (ceramics.org)
  • Nanoscaffolds support the adhesion, growth and function of various cell types as they mature into specific tissues such as tendons, muscles and bones during tissue engineering. (ceramics.org)
  • In addition, the present findings evidenced that MRI is a reliable technique to validate cell-seeding procedures in 3D porous scaffolds, and to assess the fate of hADSCs transplanted in vivo. (archives-ouvertes.fr)
  • The scaffold architecture, cell attachment and morphology were studied with scanning electron microscopy, and we assessed cell viability and functionality. (biomedsearch.com)
  • RESULTS: Cell attachment to the scaffolds was demonstrated. (biomedsearch.com)
  • Confocal laser scanning microscopy reveals that both cell distribution and extent of neuronal process alignment depend upon scaffold architecture. (illinois.edu)
  • The superficial zone-specific protein lubricin was evident on the surface of the scaffolds after 14 and 28 days of cell culture when seeded with human chondro-progenitors. (epfl.ch)
  • These results are discussed in terms of the roles of both microenvironment stiffness and cell-scaffold adhesion in cancer cell invasion. (uwaterloo.ca)
  • iPS cell derived RPE was successfully cultured as electrically intact monolayers on biodegradable scaffolds. (arvojournals.org)
  • The effect of different electrospun scaffold properties, bioreactor designs, mesenchymal stem cell culture parameters, and seeding techniques on cell behavior can be studied individually or combined with phenomenological modeling techniques. (springer.com)
  • The advantages and drawbacks of each material type will be detailed along with relevant examples of how such scaffolds can influence stem cell behavior. (stembook.org)
  • Integrated together, this suite of technologies is broadly applicable to fundamental challenges in engineering and medicine including the repair of tissue interfaces and the engineering of materials to regulate stem cell fate. (nanohub.org)
  • With the increasing understanding of the microenvironment of tumor tissues and the signaling cue-oriented cell phenotypes, many tumor biomedical studies that investigate cell signaling, gene and small-molecule expression, and drug sensitivities have adopted different three-dimensional (3D) tissue culture models ( 1 ). (sciencemag.org)
  • Cell spheroids and scaffolds are the most popular 3D tissue culture models currently used in the field. (sciencemag.org)
  • The continued success of tissue engineering and the eventual development of true human replacement parts will grow from the convergence of engineering and basic research advances in tissue, matrix, growth factor, stem cell, and developmental biology, as well as materials science and bioinformatics. (wikipedia.org)
  • Researchers at Northwestern University have developed a new method for creating scaffolds for tissue engineering applications, providing an alternative that is more flexible and less time-intensive than current technology. (medicalxpress.com)
  • These scaffolds demonstrate promise for tissue engineering applications and could potentially be used as replacements for diseased or damaged tissues. (stembook.org)
  • Hutmacher ( 1 ) gave an early analysis of the topological requirements of a suitable scaffold material and reviewed the manufacturing routes that could be used to achieve the desired structure. (sciencemag.org)
  • Fibrin, a biopolymer responsible for blood clot formation, has been shown to be suitable for tissue engineering applications. (nih.gov)
  • The overall aim of this thesis was to establish whether selected Leeds' β-structure self- assembling peptides were suitable candidates for use in skeletal tissue engineering. (bl.uk)
  • Hence, the electrospinning is a suitable tool in regenerative tissue engineering. (springer.com)
  • For me, the payoff seems to be that the FLUF method isn't limit to ePCL and should be suitable to those investigating the use of inorganic fibers for tissue engineering, too. (ceramics.org)
  • One goal of functional tissue engineering is to manufacture scaffolds infiltrated with chondrocytes which are suitable for transplantation into the lesion areas of articular cartilage. (iospress.com)
  • 2. The biomaterial scaffold of claim 1, wherein the polyelectrolyte complex fibers are comprised of a polycation precursor and a polyanion precursor. (freepatentsonline.com)
  • 3. The biomaterial scaffold of claim 2, wherein the polycation precursor is sodium alginate. (freepatentsonline.com)
  • 4. The biomaterial scaffold of claim 2 wherein reconstituted extracellular matrix is incorporated into the polycation precursor and the polyanion precursor. (freepatentsonline.com)
  • 9. The biomaterial scaffold of claim 8, wherein the animal tissue is rat liver tissue. (freepatentsonline.com)
  • 14. The biomaterial scaffold of claim 1, further comprising at least one stabilising agent. (freepatentsonline.com)
  • 20. The composite material according to claim 19 wherein the composite material comprises a constituent element of a biomaterial scaffold. (freepatentsonline.com)
  • and d) forming the scaffold from the fibers. (freepatentsonline.com)
  • Tong H-W, Wang M (2007) Electrospinning of aligned biodegradable polymer fibers and composite fibers for tissue engineering applications. (springer.com)
  • The elastic modulus of the scaffold is well maintained after the decellularization process because of the preservation of elastin fibers. (kurzweilai.net)
  • But with our 3D nanoscaffold, our rocks, or the fibers that constitute the scaffold, are the size of sand particles. (ceramics.org)
  • The sponge skeleton is a highly porous scaffold with interconnected channels formed by spongin fibers, a type of collagenous material, and calcium carbonate spicules. (arvojournals.org)
  • In vivo results allow us to consider this scaffold as a promising biomaterial to be applied in bone tissue engineering. (scirp.org)
  • All safety parameters investigated in the in vivo model (presence of localised tissue reaction, general well being of the animal, IgG levels, detection of specific anti-peptide antibodies, histological evidence of necrosis/inflammation/foreign body reaction, presence of amyloid deposits) were all negative, suggesting SAPs had low immunogenicity and were safe to use. (bl.uk)
  • Furthermore, hierarchically porous BC-silk scaffolds significantly enhanced the formation of new bone in vivo as compared to BC scaffolds. (rsc.org)
  • After implantation, cellularised scaffolds require a non-invasive method for monitoring their fate in vivo. (archives-ouvertes.fr)
  • In vivo MRI revealed that hADSCs remain detectable until 28 d after implantation and could migrate from the scaffold and colonise the area around it. (archives-ouvertes.fr)
  • Although there was no significance against the control in functionality and viability, the demonstrable attachment on scanning electron microscopy suggest potential roles for this polymer and in particular for scaffolds made with glucose particles in liver tissue engineering. (biomedsearch.com)
  • Scanning electron microscopy and confocal microscopy were used to study the structure of the scaffold during four weeks of cultivation. (iospress.com)
  • Lucas Albrecht, Stephen Sawyer, and Pranav Soman, Syracuse University, NY, present the methods used to produce polycaprolactone-based polymers and to fabricate scaffolds using a Makerbot 3D Fused Deposition Modelling printer. (eurekalert.org)
  • Projection stereolithography (PSL) offers high resolution and processing speed as well as the ability to fabricate scaffolds that precisely fit the anatomy of cartilage defects using medical imaging as the design template. (frontiersin.org)
  • This review attempts to provide recommendations on the combination of qualities that would produce the ideal scaffold system for cartilage tissue engineering. (ingentaconnect.com)
  • The ideal scaffold material must have high biocompatibility , tunable biodegradability, and nontoxic by-products [ 6 , 7 ]. (omicsonline.org)
  • The hybrid scaffolds will be useful for tissue engineering. (scientific.net)
  • In this study, high-concentration (4.8% w/v) chitosan-alginate hybrid scaffolds were successfully synthesized through a thermally-induced phase separation technique. (washington.edu)
  • The main pore sizes of the porous hybrid scaffolds was found to be 50--300 mum, which can be controlled through freezing and coalescence processes. (washington.edu)
  • The hybrid scaffolds were then implanted subcutaneously of syngeneic rats for 6 weeks. (scirp.org)
  • We then demonstrated that scaffold-mediated gene delivery of transforming growth factor β3 (TGF-β3), using a 3D woven poly(ε-caprolactone) scaffold, induced robust cartilaginous ECM formation by hMSCs. (pnas.org)
  • Laser microfabricated poly(glycerol sebacate) scaffolds for heart valve tissue engineering. (sigmaaldrich.com)
  • Microfabricated poly(glycerol sebacate) (PGS) scaffolds may be applicable to tissue engineering heart valve leaflets by virtue of their controllable microstructure, stiffness, and elasticity. (sigmaaldrich.com)
  • The utility of this approach was evaluated by using 3D poly(lactide-co-glycolide) (PLAGA) sintered microsphere scaffolds for bone tissue engineering applications. (pnas.org)
  • Therefore, our strategy to bypass this issue is to deliver the drug locally, once, using a poly(lactide-co-glycolide) (PLG) scaffold designed for extended release of resveratrol. (aiche.org)
  • fabricated poly(ε-caprolactone) (PCL) scaffolds with a honeycomb-like porous structure using an FDM-type printer. (frontiersin.org)
  • Such short self-assembling peptides that are amenable to computational design offer open-ended possibilities towards multifunctional tissue engineering scaffolds of the future. (aiche.org)
  • Integrating an advanced manufacturing technique, nanocomposite material and controlled delivery of growth factor to form multifunctional tissue engineering scaffolds was investigated in this study. (royalsocietypublishing.org)
  • Scientists have developed a new tissue 'scaffold' technology that could one day enable the engineering of large organs. (bristol.ac.uk)
  • Methods of TE have advanced significantly in recent years, but there are challenges to using engineered tissues and organs including but not limited to: biocompatibility, immunogenicity, biodegradation, and toxicity. (mdpi.com)
  • Researchers at the Universities of Bristol and Liverpool have developed a new tissue scaffold (support structure) technology that could one day make it possible to engineer large organs. (kurzweilai.net)
  • Here, different types of organs (bone, cartilage, heart valve, liver, and skin) that are aided by 3D printed scaffolds and printing methods that are applied in the biomedical fields are reviewed. (frontiersin.org)
  • In this context, designing scaffolds which mimics extracellular matrix (ECM) is essential to regenerate the damaged tissues and organs. (springer.com)
  • Scientist makes materials used in tissue scaffolds to grow artificial human organs outside the body for implantation. (sciencephoto.com)
  • A team of researchers, led by Dr Adam Perriman from the University of Bristol and Professor Anthony Hollander from the University of Liverpool, used cartilage tissue engineering as a model system for testing a new method of overcoming the oxygen limitation problem. (bristol.ac.uk)
  • The team's findings, published today [17 June] in Nature Communications , could really expand the possibilities in tissue engineering, not only in cartilage, but also for other tissue such as cardiac muscle or bone. (bristol.ac.uk)
  • Scaffolds in tissue engineering bone and cartilage. (springer.com)
  • 45S4 Bioglass-derived glass ceramic scaffolds for bone tissue engineering. (springer.com)
  • A comparative study of oxygen diffusion in tissue engineering scaffolds. (springer.com)
  • Vascularization in bone tissue engineering: physiology, current strategies, major hurdles and future challenges. (springer.com)
  • Our results indicate the potential of these scaffolds for bone tissue engineering. (hindawi.com)
  • To overcome these limitations, various bone tissue engineering strategies have been proposed. (hindawi.com)
  • However, little attention has been focused on polyphenol as a cross-linking agent for bone tissue engineering. (hindawi.com)
  • An important concept in tissue engineering is the scaffold, a three-dimensional (3D), highly porous substrate. (sciencemag.org)
  • In many ways, the engineering component of tissue engineering lies in the design and manufacture of the scaffold. (sciencemag.org)
  • In this review we summarize the current developments on scaffold systems available for cartilage tissue engineering. (ingentaconnect.com)
  • In the end of review, we conclude with an overview of the ways in which biomedical nanotechnology is widely applied in cartilage tissue engineering, especially in the design of composite scaffolds. (ingentaconnect.com)
  • In the article "Developing 3D Scaffolds in the Field of Tissue Engineering to Treat Complex Bone Defects" , the researchers report how they overcame the challenges associated with creating composite polymer spools. (eurekalert.org)
  • They discuss potential applications of these techniques, including tissue engineering to repair complex bone defects. (eurekalert.org)
  • Mary Ann Liebert, Inc., publishers is a privately held, fully integrated media company known for establishing authoritative medical and biomedical peer-reviewed journals, including Big Data, Soft Robotics, New Space, and Tissue Engineering. (eurekalert.org)
  • Many strategies for tissue engineering of replacement structures, such as heart valves, depend in part on preparing a tissue scaffold from a natural tissue matrix. (sc.edu)
  • One of the most important aspects of tissue engineering related to material science is design of the polymer scaffolds. (unt.edu)
  • The field of tissue engineering has advanced and evolved to focus on biomimetic strategies to meet the rise in demands of tissue replacements for surgical reconstruction. (bl.uk)
  • The use of scaffolds in tissue engineering is associated with various limitations such as material associated infection, mechanical failure of materials, and immunogenic reactions to implanted materials. (marketresearch.com)
  • Thus, the pivotal goal in bone tissue engineering is to reconstruct these defects. (nih.gov)
  • The team's open-access findings , published June 18 in Nature Communications , could expand the possibilities in tissue engineering, including other tissue such as cardiac muscle or bone. (kurzweilai.net)
  • West, Jennifer L. and Mann, Brenda K., "Tissue engineering scaffolds promoting matrix protein production. (rice.edu)
  • One of the fundamental principles underlying tissue engineering approaches is that newly formed tissue must maintain sufficient vascularization to support its growth. (pnas.org)
  • To overcome these limitations, tissue engineering has evolved as a means to develop viable bone grafts. (pnas.org)
  • The success of tissue engineering strategies, however, critically depends on the extent of blood vessel infiltration into the scaffolds ( 4 ). (pnas.org)
  • Funding by ImaValve (Intelligent materials for in situ heart valve tissue engineering) . (tue.nl)
  • While autologous grafting remains the gold standard for treating skin and skeletal defects, complications from antigenicity, donor site morbidity, and limited donor tissue have spurred the development of tissue engineering for regenerative therapies [ 1 , 2 ]. (omicsonline.org)
  • In this review, we discuss advances in scaffold technology for tissue engineering and highlight current trends in scaffold materials used ( Table 1 ). (omicsonline.org)
  • Bone tissue engineering aims to use biodegrade able scaffolds to replace damaged tissue. (scirp.org)
  • The biodegradability of chitosan favors its use as a biomaterial in tissue engineering and drug delivery systems. (scirp.org)
  • Moreover, for tissue engineering applications, it is important to have a predictable kinetics of the biodegradability of the polymer. (scirp.org)
  • Gellan xanthan gels have been shown to be excellent carriers for growth factors and as matrices for several tissue engineering applications. (dovepress.com)
  • Scaffold technology is integral in advancing tissue engineering and one of the tissues of interest here is the tendon/ligament. (scientific.net)
  • Advancement in the tissue engineering of tendon/ligament has become very much a materials engineering problem than ever, with the selection of appropriate biomaterial and scaffold architecture. (scientific.net)
  • 2] Cato T. Laurencin, Joseph W. Freeman: Ligament Tissue Engineering: An Evolutionary Material Science Approach. (scientific.net)
  • 3] Bell E.: Strategy for Selection of Scaffolds for Tissue Engineering. (scientific.net)
  • The SphereScaff project aimed at producing tissue engineering scaffolds via 3D printing of microspheres. (europa.eu)
  • The results suggest that they as good as the market leads in inducing bone repair, their biocompatibilitv and low immunogenicity strongly support their further development for use in human tissue engineering applications. (bl.uk)
  • Through tissue engineering, researchers seek to regenerate human tissue , such as bone and cartilage , that has been damaged by injury or disease. (medicalxpress.com)
  • Fracture toughness has occasionally been neglected in the development of tissue engineering scaffolds. (cam.ac.uk)
  • In recent years, a new cutting-edge procedure, bone- and cartilage-tissue engineering, has emerged as a new strategy for healing musculoskeletal conditions. (washington.edu)
  • The results support the potential applications of chitosan-alginate scaffolds as an alternative to other natural polymer-based scaffolds in tissue engineering. (washington.edu)
  • Hutmacher DW (2000) Scaffolds in tissue engineering bone and cartilage. (springer.com)
  • Kidoaki S, Kwon IK, Matsuda T (2005) Mesoscopic spatial designs of nano- and microfiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques. (springer.com)
  • Methods for preparation and use of these scaffolds as tissue engineering devices are also provided. (freepatentsonline.com)
  • Tissue Engineering: Part A, 18, 1677-1685. (scirp.org)
  • R. (2005) Tissue Engineering of Bone: Search for a Better Scaffold. (scirp.org)
  • Polymer blending is an effective method for developing, desirable bio-composites for tissue engineering. (amrita.edu)
  • Applications are invited for postgraduate research leading to a PhD degree in Biomedical Engineering within the subject area of Tissue Engineering. (findaphd.com)
  • in addition they will be important for an academic career in Tissue Engineering/Biomedical Engineering. (findaphd.com)
  • Both materials are well known to be promising candidates for tissue engineering and drug delivery applications. (upc.edu)
  • However, a group of investigators the University of Alabama at Birmingham's School of Engineering have come up with an ingenious method of using electrospinning to build a superior three-dimensional scaffold of nanofibers that, it is hoped, will provide a boost to tissue engineering. (ceramics.org)
  • Thus, research in tissue engineering still remains very active but it has failed to realize viable commercial products. (royalsocietypublishing.org)
  • Three-dimensional (3D) microperiodic scaffolds have been fabricated by direct-write assembly for tissue engineering. (illinois.edu)
  • Which is very difficult to achieve, so recently we have developed an approach to using very high tech tissue engineering. (hstalks.com)
  • In this work the research group developed a new composite material that can be used for tissue engineering, specifically for regenerating bone tissue. (phys.org)
  • A. Reizabal et al, Silk fibroin magnetoactive nanocomposite films and membranes for dynamic bone tissue engineering strategies, Materialia (2020). (phys.org)
  • New approaches to engineering tissues, such as 3D bioprinting, are described as they provide exciting opportunities for future work along with current challenges that must be addressed. (iospress.com)
  • We have investigated the suitability of viscose cellulose sponges as a scaffold for cartilage tissue engineering. (iospress.com)
  • Tissue engineering scaffolds have been developed to repair and replace different types of tissues. (utexas.edu)
  • Steven Caliari received his bachelor's degree in chemical engineering from the University of Florida in 2007 and following graduation worked at RTI Biologics, a small tissue engineering company. (nanohub.org)
  • For scaffold-based tissue engineering, although ideal scaffolds have not been achieved yet (or actually there are no such ideal models and each design should follow specific tissue requirements), certain minimum criteria should be met, viz . (royalsocietypublishing.org)
  • Play media While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc. (wikipedia.org)
  • Among the major challenges now facing tissue engineering is the need for more complex functionality, biomechanical stability, and vascularization in laboratory-grown tissues destined for transplantation. (wikipedia.org)
  • In 2003, the NSF published a report entitled "The Emergence of Tissue Engineering as a Research Field", which gives a thorough description of the history of this field. (wikipedia.org)
  • When applied as a filler for soft tissue defects and voids, it shows promise for accelerating and improving tissue restoration outcomes. (purdue.edu)
  • Such an approach may also benefit other patient populations in need of soft tissue restoration or reconstruction, including children with congenital defects, individuals with difficult-to-heal skin ulcers, individuals suffering from traumatic injuries and cancer patients requiring resection of tumors within tissues other than breast. (purdue.edu)
  • This scaffold has soft tissue consistency and persists, where it induces a regenerative healing response. (purdue.edu)
  • Tissue-Engineered Soft-Tissue Reconstruction Using Noninvasive Mechanical Preconditioning and a Shelf-Ready Allograft Adipose Matrix. (umassmed.edu)
  • Here we developed new photopolymerizable HA based materials for soft tissue repair application. (utexas.edu)
  • Review of polymer-based scaffolds revealed that a composite scaffold formed by copolymerization is more effective than single polymer scaffolds because it allows copolymers to offset disadvantages a single polymer may possess. (mdpi.com)
  • The use of tissue scaffolds in clinical applications is still in its relatively early stage. (marketresearch.com)
  • This event will discuss the latest technologies to recreate the 3D tissue environment for medical research and therapeutic applications. (cam.ac.uk)
  • Precisely controlling the macro- and micro-architecture of the scaffold and hence fulfilling a custom design with complex anatomic shapes are of significant importance for clinical applications of the scaffold. (royalsocietypublishing.org)
  • Scaffolds derived from decellularized cardiac tissue offer an enormous advantage for cardiac applications as they recapitulate biophysical and cardiac specific cues. (diva-portal.org)
  • At the end of the experiment, there was no significant difference in viability and functionality between the scaffolds and the control. (biomedsearch.com)
  • However, their high cytotoxicity may influence the biocompatibility of the scaffolds [ 19 ]. (hindawi.com)