Tissue Inhibitor of Metalloproteinase-1: A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It is a N-glycosylated protein, molecular weight 28 kD, produced by a vast range of cell types and found in a variety of tissues and body fluids. It has been shown to suppress metastasis and inhibit tumor invasion in vitro.Tissue Inhibitor of Metalloproteinases: A family of secreted protease inhibitory proteins that regulates the activity of SECRETED MATRIX METALLOENDOPEPTIDASES. They play an important role in modulating the proteolysis of EXTRACELLULAR MATRIX, most notably during tissue remodeling and inflammatory processes.Tissue Inhibitor of Metalloproteinase-2: A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It is a 21-kDa nonglycosylated protein found in tissue fluid and is secreted as a complex with progelatinase A by human fibroblast and uncomplexed from alveolar macrophages. An overexpression of TIMP-2 has been shown to inhibit invasive and metastatic activity of tumor cells and decrease tumor growth in vivo.Tissue Inhibitor of Metalloproteinase-3: A member of the family of tissue inhibitor of metalloproteinases. Mutations of the gene for TIMP3 PROTEIN causes Sorsby fundus dystrophy.Matrix Metalloproteinase 2: A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.Matrix Metalloproteinase 9: An endopeptidase that is structurally similar to MATRIX METALLOPROTEINASE 2. It degrades GELATIN types I and V; COLLAGEN TYPE IV; and COLLAGEN TYPE V.Metalloendopeptidases: ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism.Matrix Metalloproteinases: A family of zinc-dependent metalloendopeptidases that is involved in the degradation of EXTRACELLULAR MATRIX components.Gelatinases: A class of enzymes that catalyzes the degradation of gelatin by acting on the peptide bonds. EC 3.4.24.-.Matrix Metalloproteinase 3: An extracellular endopeptidase of vertebrate tissues similar to MATRIX METALLOPROTEINASE 1. It digests PROTEOGLYCAN; FIBRONECTIN; COLLAGEN types III, IV, V, and IX, and activates procollagenase. (Enzyme Nomenclature, 1992)Matrix Metalloproteinase 1: A member of the metalloproteinase family of enzymes that is principally responsible for cleaving FIBRILLAR COLLAGEN. It can degrade interstitial collagens, types I, II and III.Matrix Metalloproteinase Inhibitors: Compounds that inhibit the enzyme activity or activation of MATRIX METALLOPROTEINASES.Collagenases: Enzymes that catalyze the degradation of collagen by acting on the peptide bonds.Matrix Metalloproteinases, Membrane-Associated: Matrix metalloproteinases that are associated with the CELL MEMBRANE, either through transmembrane domains or GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORS. Membrane-type matrix metalloproteinases may act within the pericellular environment to influence the process of CELL MIGRATION.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Matrix Metalloproteinase 8: A member of the MATRIX METALLOPROTEINASES that cleaves triple-helical COLLAGEN types I, II, and III.Metalloproteases: Proteases which use a metal, normally ZINC, in the catalytic mechanism. This group of enzymes is inactivated by metal CHELATORS.Enzyme Precursors: Physiologically inactive substances that can be converted to active enzymes.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Microbial Collagenase: A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC 3.4.24.3.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.Matrix Metalloproteinase 7: The smallest member of the MATRIX METALLOPROTEINASES. It plays a role in tumor progression.Matrix Metalloproteinases, Secreted: A subclass of matrix metalloproteinases that are secreted into the pericellular space.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Matrix Metalloproteinase 14: A transmembrane domain-containing matrix metalloproteinase. It is synthesized as an inactive zymogen that is activated by the action of PROPROTEIN CONVERTASES such as FURIN. Matrix metalloproteinase 14 plays a direct role in the cleavage of proteins in the pericellular environment. In addition, it can function indirectly by enzymatically activating the proprotein form of MATRIX METALLOPROTEINASE 15.Matrix Metalloproteinase 13: A secreted matrix metalloproteinase that plays a physiological role in the degradation of extracellular matrix found in skeletal tissues. It is synthesized as an inactive precursor that is activated by the proteolytic cleavage of its N-terminal propeptide.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Matrix Metalloproteinase 10: A secreted matrix metalloproteinase that may play a role in matrix degradation during WOUND HEALING. It is expressed at high levels by KERATINOCYTES, suggesting its role in keratinocyte migration.Phenylmercuric Acetate: A phenyl mercury compound used mainly as a fungicide. Has also been used as a herbicide, slimicide, and bacteriocide.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.ADAM Proteins: A family of membrane-anchored glycoproteins that contain a disintegrin and metalloprotease domain. They are responsible for the proteolytic cleavage of many transmembrane proteins and the release of their extracellular domain.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Matrix Metalloproteinase 16: A transmembrane domain-containing matrix metalloproteinase. It is synthesized as an inactive zymogen that is activated by the proteolytic action of PROPROTEIN CONVERTASES. Matrix metalloproteinase 16 plays a direct role in the cleavage of proteins in the pericellular environment. In addition, it can function indirectly by enzymatically activating the proprotein form of other MATRIX METALLOPROTEINASES such as the zymogen of MATRIX METALLOPROTEINASE 2.Gelatin: A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Pepsin A: Formed from pig pepsinogen by cleavage of one peptide bond. The enzyme is a single polypeptide chain and is inhibited by methyl 2-diaazoacetamidohexanoate. It cleaves peptides preferentially at the carbonyl linkages of phenylalanine or leucine and acts as the principal digestive enzyme of gastric juice.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Matrix Metalloproteinase 15: A transmembrane domain-containing matrix metalloproteinase that plays a role in the cleavage of proteins in the pericellular environment. It is synthesized as an inactive zymogen that is activated by the action of ENDOPEPTIDASES such as MATRIX METALLOPROTEINASE 14.Collagen Type I: The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.Matrix Metalloproteinase 12: A secreted matrix metalloproteinase which is highly expressed by MACROPHAGES where it may play a role in INFLAMMATION and WOUND HEALING.Neoplasm Invasiveness: Ability of neoplasms to infiltrate and actively destroy surrounding tissue.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Matrix Metalloproteinase 11: A secreted matrix metalloproteinase that is believed to play a role in EXTRACELLULAR MATRIX remodeling and cell fate determination during normal and pathological processes. Matrix metalloproteinase 11 was originally isolated in primary BREAST NEOPLASMS and may be involved in the process of tumorigenesis.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Extracellular Matrix Proteins: Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).Fibrosis: Any pathological condition where fibrous connective tissue invades any organ, usually as a consequence of inflammation or other injury.Culture Media, Conditioned: Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).Collagen Type III: A fibrillar collagen consisting of three identical alpha1(III) chains that is widely distributed in many tissues containing COLLAGEN TYPE I. It is particularly abundant in BLOOD VESSELS and may play a role in tissues with elastic characteristics.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Transforming Growth Factor beta: A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.Ventricular Remodeling: The geometric and structural changes that the HEART VENTRICLES undergo, usually following MYOCARDIAL INFARCTION. It comprises expansion of the infarct and dilatation of the healthy ventricle segments. While most prevalent in the left ventricle, it can also occur in the right ventricle.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Interleukin-1: A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Oncostatin M: A cytokine with both pro- and anti-inflammatory actions that depend upon the cellular microenvironment. Oncostatin M is a 28 kDa monomeric glycoprotein that is similar in structure to LEUKEMIA INHIBITORY FACTOR. Its name derives from the the observation that it inhibited the growth of tumor cells and augmented the growth of normal fibroblasts.Liver Cirrhosis: Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules.Transforming Growth Factor beta1: A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Procollagen N-Endopeptidase: An extracellular endopeptidase which excises a block of peptides at the amino terminal, nonhelical region of the procollagen molecule with the formation of collagen. Absence or deficiency of the enzyme causes accumulation of procollagen which results in the inherited connective tissue disorder--dermatosparaxis. EC 3.4.24.14.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Synovial Membrane: The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Pentosan Sulfuric Polyester: A sulfated pentosyl polysaccharide with heparin-like properties.In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Liver Cirrhosis, Experimental: Experimentally induced chronic injuries to the parenchymal cells in the liver to achieve a model for LIVER CIRRHOSIS.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.HemopexinDisease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Mice, Inbred C57BLNeoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Autoimmune Lymphoproliferative Syndrome: Rare congenital lymphoid disorder due to mutations in certain Fas-Fas ligand pathway genes. Known causes include mutations in FAS, TNFSF6, NRAS, CASP8, and CASP10 proteins. Clinical features include LYMPHADENOPATHY; SPLENOMEGALY; and AUTOIMMUNITY.Procollagen: A biosynthetic precursor of collagen containing additional amino acid sequences at the amino-terminal and carboxyl-terminal ends of the polypeptide chains.Urokinase-Type Plasminogen Activator: A proteolytic enzyme that converts PLASMINOGEN to FIBRINOLYSIN where the preferential cleavage is between ARGININE and VALINE. It was isolated originally from human URINE, but is found in most tissues of most VERTEBRATES.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Collagen Type IV: A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer.Hepatic Stellate Cells: Perisinusoidal cells of the liver, located in the space of Disse between HEPATOCYTES and sinusoidal endothelial cells.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Cartilage, Articular: A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.Endometrium: The mucous membrane lining of the uterine cavity that is hormonally responsive during the MENSTRUAL CYCLE and PREGNANCY. The endometrium undergoes cyclic changes that characterize MENSTRUATION. After successful FERTILIZATION, it serves to sustain the developing embryo.Aggrecans: Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Disease Progression: The worsening of a disease over time. This concept is most often used for chronic and incurable diseases where the stage of the disease is an important determinant of therapy and prognosis.ElastinAdenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Hydroxyproline: A hydroxylated form of the imino acid proline. A deficiency in ASCORBIC ACID can result in impaired hydroxyproline formation.Leukocyte Elastase: An enzyme that catalyzes the hydrolysis of proteins, including elastin. It cleaves preferentially bonds at the carboxyl side of Ala and Val, with greater specificity for Ala. EC 3.4.21.37.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Corpus Luteum: The yellow body derived from the ruptured OVARIAN FOLLICLE after OVULATION. The process of corpus luteum formation, LUTEINIZATION, is regulated by LUTEINIZING HORMONE.Fibrosarcoma: A sarcoma derived from deep fibrous tissue, characterized by bundles of immature proliferating fibroblasts with variable collagen formation, which tends to invade locally and metastasize by the bloodstream. (Stedman, 25th ed)Extraembryonic Membranes: The thin layers of tissue that surround the developing embryo. There are four extra-embryonic membranes commonly found in VERTEBRATES, such as REPTILES; BIRDS; and MAMMALS. They are the YOLK SAC, the ALLANTOIS, the AMNION, and the CHORION. These membranes provide protection and means to transport nutrients and wastes.Bruch Membrane: The inner layer of CHOROID, also called the lamina basalis choroideae, located adjacent to the RETINAL PIGMENT EPITHELIUM; (RPE) of the EYE. It is a membrane composed of the basement membranes of the choriocapillaris ENDOTHELIUM and that of the RPE. The membrane stops at the OPTIC NERVE, as does the RPE.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Synovial Fluid: The clear, viscous fluid secreted by the SYNOVIAL MEMBRANE. It contains mucin, albumin, fat, and mineral salts and serves to lubricate joints.Cell Line: Established cell cultures that have the potential to propagate indefinitely.ThiophenesCell Line, Tumor: A cell line derived from cultured tumor cells.Carbon Tetrachloride: A solvent for oils, fats, lacquers, varnishes, rubber waxes, and resins, and a starting material in the manufacturing of organic compounds. Poisoning by inhalation, ingestion or skin absorption is possible and may be fatal. (Merck Index, 11th ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Enzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.Cytokines: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.Chondrocytes: Polymorphic cells that form cartilage.Neoplasm Metastasis: The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Osteoarthritis: A progressive, degenerative joint disease, the most common form of arthritis, especially in older persons. The disease is thought to result not from the aging process but from biochemical changes and biomechanical stresses affecting articular cartilage. In the foreign literature it is often called osteoarthrosis deformans.Stromal Cells: Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Central Nervous System Helminthiasis: Infections of the BRAIN; SPINAL CORD; or MENINGES caused by HELMINTHS (parasitic worms).Vascular Endothelial Growth Factor A: The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.Relaxin: A water-soluble polypeptide (molecular weight approximately 8,000) extractable from the corpus luteum of pregnancy. It produces relaxation of the pubic symphysis and dilation of the uterine cervix in certain animal species. Its role in the human pregnant female is uncertain. (Dorland, 28th ed)Cartilage: A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.Fibril-Associated Collagens: A family of non-fibrillar collagens that interact with FIBRILLAR COLLAGENS. They contain short triple helical domains interrupted by short non-helical domains and do not form into collagen fibrils.Ovulation: The discharge of an OVUM from a rupturing follicle in the OVARY.Basement Membrane: A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.Nested Genes: Genes whose entire sequences are contained within other genes.Laminin: Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.Scleroderma, Localized: A term used to describe a variety of localized asymmetrical SKIN thickening that is similar to those of SYSTEMIC SCLERODERMA but without the disease features in the multiple internal organs and BLOOD VESSELS. Lesions may be characterized as patches or plaques (morphea), bands (linear), or nodules.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Neovascularization, Pathologic: A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Kinetics: The rate dynamics in chemical or physical systems.Tumor Markers, Biological: Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Osteopontin: A negatively-charged extracellular matrix protein that plays a role in the regulation of BONE metabolism and a variety of other biological functions. Cell signaling by osteopontin may occur through a cell adhesion sequence that recognizes INTEGRIN ALPHA-V BETA-3.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.Dipeptides: Peptides composed of two amino acid units.Phenylalanine: An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE.Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Gingiva: Oral tissue surrounding and attached to TEETH.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Rats, Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.Connective Tissue Growth Factor: A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It is found in hypertrophic CHONDROCYTES where it may play a role in CHONDROGENESIS and endochondral ossification.Immunoblotting: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.Joint Capsule: The sac enclosing a joint. It is composed of an outer fibrous articular capsule and an inner SYNOVIAL MEMBRANE.Trophoblasts: Cells lining the outside of the BLASTOCYST. After binding to the ENDOMETRIUM, trophoblasts develop into two distinct layers, an inner layer of mononuclear cytotrophoblasts and an outer layer of continuous multinuclear cytoplasm, the syncytiotrophoblasts, which form the early fetal-maternal interface (PLACENTA).
Destroy host tissues: Inhibit host repair of tissues: Adhesins Leukotoxin Cytotoxins Inhibitors of fibroblast proliferation ... Episodic nature of attachment loss: Two main tissue responses have been found in GAP cases:[31] *Tissue may have severe acute ... The amount of plaque present is inconsistent with the amount and severity of tissue destruction [26][27] but with a high plaque ... The other response is known as a period of quiescence, where gingival tissue may appear with no inflammation, pink appearance ...
Main article: Brown adipose tissue. Brown fat or brown adipose tissue (BAT) is a specialized form of adipose tissue important ... The two types of adipose tissue are white adipose tissue (WAT), which stores energy, and brown adipose tissue (BAT), which ... In biology, adipose tissue, body fat, or simply fat is a loose connective tissue composed mostly of adipocytes.[1] In addition ... Adipose tissue has a density of ~0.9 g/ml.[90] Thus, a person with more adipose tissue will float more easily than a person of ...
... and by endogenous inhibitors known as tissue inhibitors of metalloproteases (TIMPs). The role of matrix metalloproteases and ... Murphy, G; Willenbrock, F (1995). "Tissue inhibitors of matrix metalloendopeptidases". Methods Enzymol. Methods in Enzymology. ... Inhibitors of CD13/APN dramatically impair tumor growth. It has become clear in the past years that ectodomain shedding is an ... Endogenous inhibitors or activators generated by proteolytic degradation of larger proteins mostly from the ECM have proven to ...
... tissue factor pathway inhibitor precursor; and Kunitz STI protease inhibitor contained in legume seeds. Kunitz domains are ... bovine pancreatic trypsin inhibitor, BPTI), Alzheimer's amyloid precursor protein (APP), and tissue factor pathway inhibitor ( ... Salier JP (1990). "Inter-alpha-trypsin inhibitor: emergence of a family within the Kunitz-type protease inhibitor superfamily ... and partial characterization of a second human tissue-factor-pathway inhibitor". Proc. Natl. Acad. Sci. U.S.A. 91 (8): 3353- ...
... soft tissue injury treatment. EMR Licence Endorsements: maintenance of intravenous lines without medications or blood products ... platelet inhibitors; use and interpretation of a glucometer; chest auscultation. (f) insertion and maintenance of ...
COX-2 inhibitors[edit]. Main article: COX-2 inhibitor. These drugs have been derived from NSAIDs. The cyclooxygenase enzyme ... Soft tissue inflammation and pain.. As per bufexamac. Flurbiprofen. Comes in sodium salt and free acid forms; fairly insoluble ... COX-2 selective inhibitors Celecoxib. Comes in free form; practically insoluble in water, fairly soluble in organic solvents. ... Selective COX-2 inhibitor.. PO.. Protein binding = 97%; hepatic metabolism, mostly via CYP2C9; faeces (57%), urine (27%).[81]. ...
Castora, F. J.; Vissering, F. F.; Simpson, M. V. (September 1983). "The effect of bacterial DNA gyrase inhibitors on DNA ... Bergan T; Dalhoff A; Thorsteinsson SB (1985). "A review of the pharmacokinetics and tissue penetration of ciprofloxacin": 23-36 ... due to their ability to enter bone tissue without chelating it, as tetracyclines are known to do. ... "Fluoroquinolones vs beta-lactams for empirical treatment of immunocompetent patients with skin and soft tissue infections: a ...
TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is ... Nothnick WB, Soloway P, Curry TE (May 1997). "Assessment of the role of tissue inhibitor of metalloproteinase-1 (TIMP-1) during ... Hornebeck W (December 2003). "Down-regulation of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) in aged human skin ... Nothnick WB (September 2000). "Disruption of the tissue inhibitor of metalloproteinase-1 gene results in altered reproductive ...
"Studies on the Mechanism of Hydrogen Transport in Animal Tissues : Vi. Inhibitor Studies with Succinic Dehydrogenase". The ... The observation that malonate is a competitive inhibitor of succinate dehydrogenase was used to deduce the structure of the ... I. Spectral properties of the purified enzyme and formation of enzyme-competitive inhibitor complexes". Biochim. Biophys. Acta ... Malonate is a competitive inhibitor of the enzyme succinate dehydrogenase: malonate binds to the active site of the enzyme ...
Coagulation inhibitors. *Antithrombin (inhibits II, IX, X, XI, XII). *Protein C (inhibits V, VIII)/Protein S (cofactor for ... Tissue factor also binds to factor VII in the blood, which initiates the intrinsic coagulation cascade to increase thrombin ... NETs bind tissue factor, binding the coagulation centres to the location of infection. They also activate the intrinsic ... The platelets congregate around the wound in order to create a cap to stop blood flow out of the tissue. ...
... factor Xa and tissue factor in the quaternary complex tissue factor-factor VIIa-factor Xa-tissue factor pathway inhibitor". ... The action of the factor is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after ... Golino P (May 2002). "The inhibitors of the tissue factor:factor VII pathway". Thrombosis Research. 106 (3): V257-65. doi: ... Tissue factor is found on the outside of blood vessels - normally not exposed to the bloodstream. Upon vessel injury, tissue ...
... agents are pharmaceutical or herbal medicines that protect mucous membrane tissues. They include such things as ... "Effect of Althaea officinalis on cough associated with ACE inhibitors." Pakistan Journal of Nutrition 6.3 (2007): 256-258. Boyd ...
Chao J, Miao RQ, Chen V, Chen LM, Chao L (January 2001). "Novel roles of kallistatin, a specific tissue kallikrein inhibitor, ... The MEROPS online database for peptidases and their inhibitors: A01.009. *GeneReviews/NIH/NCBI/UW entry on Neuronal Ceroid- ... Umezawa H, Aoyagi T, Morishima H, Matsuzaki M, Hamada M (May 1970). "Pepstatin, a new pepsin inhibitor produced by ... processing of enzyme activators and inhibitors, brain antigen processing and regulation of programmed cell death.[17][18][19][ ...
1988). «Expression of pancreatic secretory trypsin inhibitor gene in neoplastic tissues.». FEBS Lett. 225 (1-2): 113-9. PMID ... endopeptidase inhibitor activity. •serine-type endopeptidase inhibitor activity. •protein binding. Componente celular. •região ... Schneider A (2005). «Serine protease inhibitor kazal type 1 mutations and pancreatitis.». Clin. Lab. Med. 25 (1): 61-78. PMID ... 1988). «Primary structure of human pancreatic secretory trypsin inhibitor (PSTI) gene.». Biochem. Biophys. Res. Commun. 149 (2 ...
Maskos K, Bode W (November 2003). "Structural basis of matrix metalloproteinases and tissue inhibitors of metalloproteinases". ... Gross J, Lapiere CM (June 1962). "Collagenolytic activity in amphibian tissues: a tissue culture assay". Proc. Natl. Acad. Sci ... Krane SM (1995). "Is collagenase (matrix metalloproteinase-1) necessary for bone and other connective tissue remodeling?". Clin ... The MEROPS online database for peptidases and their inhibitors: M10.001 Molecular and Cellular Biology portal. ...
This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer ... Järvinen M, Rinne A, Hopsu-Havu VK (1988). "Human cystatins in normal and diseased tissues--a review". Acta Histochem. 82 (1): ... Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory ... Turk V, Bode W (1991). "The cystatins: protein inhibitors of cysteine proteinases". FEBS Lett. 285 (2): 213-9. doi:10.1016/0014 ...
Agapova OA, Ricard CS, Salvador-Silva M, Hernandez MR (2001). "Expression of matrix metalloproteinases and tissue inhibitors of ... Belaaouaj AA, Li A, Wun TC (2000). "Matrix metalloproteinases cleave tissue factor pathway inhibitor. Effects on coagulation". ... 2001). "Crystal structure of human macrophage elastase (MMP-12) in complex with a hydroxamic acid inhibitor". J. Mol. Biol. 312 ... The MEROPS online database for peptidases and their inhibitors: M10.009 Molecular and Cellular Biology portal. ...
1996). "Kallistatin, a novel human tissue kallikrein inhibitor: levels in body fluids, blood cells, and tissues in health and ... Zhou GX, Chao L, Chao J (1993). "Kallistatin: a novel human tissue kallikrein inhibitor. Purification, characterization, and ... 2001). "Novel roles of kallistatin, a specific tissue kallikrein inhibitor, in vascular remodeling". Biol. Chem. 382 (1): 15-21 ... a novel human serine proteinase inhibitor. Molecular cloning, tissue distribution, and expression in Escherichia coli". J Biol ...
Not all inhibitors of oxidative phosphorylation are toxins. In brown adipose tissue, regulated proton channels called ... Lambert AJ, Brand MD (2004). "Inhibitors of the quinone-binding site allow rapid superoxide production from mitochondrial NADH: ... Spectral properties of the purified enzyme and formation of enzyme-competitive inhibitor complexes". Biochim. Biophys. Acta. 92 ...
... is a matrix metalloproteinase inhibitor (MMPI). Excision of malignant tumors comprises first line treatment for ... cancer of solid tissues. This procedure not infrequently misses small fragments of the tumor that may have broken off before ... Batimastat Marimastat Coussens, L. M. (2002). "Matrix Metalloproteinase Inhibitors and Cancer--Trials and Tribulations". ...
A histone deacetylase inhibitor Trichostatin A increased levels of TET1 in breast cancer tissues but was a less effective tumor ... "TET1 suppresses cancer invasion by activating the tissue inhibitors of metalloproteinases". Cell Reports. 2 (3): 568-79. doi: ... "TET1 partially mediates HDAC inhibitor-induced suppression of breast cancer invasion". www.spandidos-publications.com. ... "TET1 is a tumour suppressor that inhibits colon cancer growth by derepressing inhibitors of the WNT pathway". Oncogene. 34 (32 ...
... inhibitors show great potential in the treatment of osteoporosis. Cathepsin K is degraded by Cathepsin S, called ... 1996). "Evaluation of the extent of the binding site in human tissue kallikrein by synthetic substrates with sequences of human ... 1998). "Design of potent and selective human cathepsin K inhibitors that span the active site". Proc. Natl. Acad. Sci. U.S.A. ... McGrath ME, Klaus JL, Barnes MG, Brömme D (1997). "Crystal structure of human cathepsin K complexed with a potent inhibitor". ...
1996). "Inhibitory properties of a novel human Kunitz-type protease inhibitor homologous to tissue factor pathway inhibitor". ... 1999). "Matrix localization of tissue factor pathway inhibitor-2/matrix-associated serine protease inhibitor (TFPI-2/MSPI) ... cDNA cloning of the 33-kDa inhibitor reveals its identity to tissue factor pathway inhibitor-2". Arch. Biochem. Biophys. 335 (1 ... Tissue factor pathway inhibitor 2 is a protein that in humans is encoded by the TFPI2 gene. GRCh38: Ensembl release 89: ...
Sodium tungstate is a competitive inhibitor of molybdenum. Dietary tungsten reduces the concentration of molybdenum in tissues ... However, an extremely high concentration of molybdenum reverses the trend and can act as an inhibitor in both purine catabolism ...
... are binding to body tissue; damaging body tissue. In some cases physical urticaria can be a symptom of an underlying health ... Urticarial syndromes Acquired C1 esterase inhibitor deficiency List of cutaneous conditions Rapini, Ronald P.; Bolognia, Jean L ...
... dipeptidyl peptidase-4 inhibitors, SGLT2 inhibitors, and glucagon-like peptide-1 analogs.[87] As of 2015 there was no ... and fat tissue.[51] In the liver, insulin normally suppresses glucose release. However, in the setting of insulin resistance, ... There is some evidence that angiotensin converting enzyme inhibitors (ACEIs) are superior to other inhibitors of the renin- ... 2015 American Diabetes Association recommendations are that people with diabetes and albuminuria should receive an inhibitor of ...
The Tissue Inhibitors of Metalloproteinases (TIMPs) are a family of secreted proteins whose primary function is to limit the ... degradative actions of the matrix metalloproteinases (MMPs) during tissue... ... 1997) Murine tissue inhibitor of metalloproteinases-4 (Timp-4): cDNA isolation and expression in adult mouse tissues. FEBS ... 1998) Synthetic matrix metalloproteinase inhibitors and tissue inhibitor of metalloproteinase -2 (TIMP-2), but not TIMP-1, ...
1 Abstracts with Tissue Inhibitors of Metalloproteinases (TIMPs) Research. Filter by Study Type. Human: Case Report. ... 2 Diseases Researched for Tissue Inhibitors of Metalloproteinases (TIMPs) Name. AC. CK. Focus. ... 1 Substances Researched for Tissue Inhibitors of Metalloproteinases (TIMPs) Name. AC. CK. Focus. ... Cannabinoids inhibit glioma (brain cancer) through the down-regulation of Tissue Inhibitors of Metalloproteinases (TIMPs). Jan ...
... is a factor Xa dependent inhibitor of tissue factor initiated blood coagulation. In recent years several alternatively spliced ... Tissue factor pathway inhibitor (TFPI) is a factor Xa dependent inhibitor of tissue factor initiated blood coagulation. In ... Alternatively spliced tissue factor pathway inhibitor: functional implications Front Biosci (Schol Ed). 2011 Jun 1;3:1457-62. ... They are differentially expressed in human and mouse tissues and may have distinct physiological functions. ...
... Summary. Summary: A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It ... tissue inhibitor of metalloproteinases*gelatinases*tissue inhibitor of metalloproteinase 3*extracellular matrix*neoplasm ... tissue inhibitor of metalloproteinases , tissue inhibitor of metalloproteinase 2 ... Tissue inhibitor of metalloproteinase 2 (TIMP-2) is an angiogenesis inhibitor initially characterized for its ability to block ...
Expression profiling of metalloproteinases and tissue inhibitors of metalloproteinases in normal and degenerate human achilles ... 4 genes of tissue inhibitors of metalloproteinases (TIMPs), and ADAM genes 8, 10, 12, and 17 in normal, painful, and ruptured ...
Visceral adipose tissue-derived serine protease inhibitor: A unique insulin-sensitizing adipocytokine in obesity. Kazuyuki Hida ... Visceral Adipose Tissue-derived Serine Proteinase Inhibitor Inhibits Apoptosis of Endothelial Cells as a Ligand for the Cell- ... Visceral adipose tissue-derived serine protease inhibitor: A unique insulin-sensitizing adipocytokine in obesity ... Visceral adipose tissue-derived serine protease inhibitor: A unique insulin-sensitizing adipocytokine in obesity ...
Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) (ab108904) has been cited in 1 publications. References for Human in ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ... Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By research area. Cancer. Cardiovascular. ...
... tissue collagenase), MMP-2 (gelatinase A), MMP-3 (stromelysin-1), MMP-9 (gelatinase B), tissue inhibitors of metalloproteinase ... We examined production and tissue localization of matrix metalloproteinase (MMP)-1 ( ... tissue collagenase), MMP-2 (gelatinase A), MMP-3 (stromelysin-1), MMP-9 (gelatinase B), tissue inhibitors of metalloproteinase ... Production of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human breast carcinomas Jpn J Cancer Res ...
Tissue factor and tissue factor pathway inhibitor are important in extrinsic coagulation. We investigated their clinical ... and determined tissue factor and tissue factor pathway inhibitor levels by ELISA. In the patients the tissue factor level was ... Tissue factor and tissue factor pathway inhibitor are important in extrinsic coagulation. We investigated their clinical ... tissue factor pathway inhibitor levels were significantly higher than in those without it. Therefore, the tissue factor pathway ...
1991) Decreased tissue inhibitor of metalloproteinases (TIMP) in abdominal aortic aneurysm tissue: A preliminary report. J Surg ... Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Polymorphisms in a Caucasian Population with Abdominal Aortic Aneurysm. ... 2003) Tissue inhibitor of metalloproteinases-1, -2, and -3 polymorphisms in a white population with intracranial aneurysms. ... Their activity is controlled by tissue inhibitors of metalloproteinases (TIMPs). Decreased TIMP-1 and TIMP-2 expression in the ...
Tissue factor pathway inhibitor (or TFPI) is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa). While Xa ... Tissue factor pathway inhibitor has been shown to interact with Factor X. Hemostasis GRCh38: Ensembl release 89: ... Higuchi DA, Wun TC, Likert KM, Broze GJ (1992). "The effect of leukocyte elastase on tissue factor pathway inhibitor". Blood. ... 1995). "The carboxy terminus of tissue factor pathway inhibitor is required for interacting with hepatoma cells in vitro and in ...
Tissue Inhibitor of Metalloproteinases at the US National Library of Medicine Medical Subject Headings (MeSH). ... Brew K, Dinakarpandian D, Nagase H (2000). "Tissue inhibitors of metalloproteinases: evolution, structure and function". ... The matrix metalloproteinases are inhibited by specific endogenous tissue inhibitors of metalloproteinases (TIMPs), which ... comprise a family of four protease inhibitors: TIMP1, TIMP2, TIMP3 and TIMP4. Overall, all MMPs are inhibited by TIMPs once ...
Direct thrombin inhibitors, Myocardial infarction, Platelet-monocyte aggregates, Tissue factor National Category Medical and ... The Influence of Direct Thrombin Inhibitors on the Formation of Platelet-leukocyte Aggregates and Tissue Factor Expression. ... Both in vivo and in vitro the direct thrombin inhibitor diminished procoagulant activity and tissue factor (TF) presenting ... Open this publication in new window or tab ,,Regulation of Tissue Factor and Coagulation Activity: Translation Studies with ...
Serum Levels of Tissue Inhibitors of Metalloproteinase 2 in Patients With Systemic Sclerosis With Duration More Than 2 Years: ... Amira Shahin, Amani Elsawaf, Shahira Ramadan, Olfat Shaker, Mona Amin, and Mohamed Taha, "Serum Levels of Tissue Inhibitors of ...
Mixed connective-tissue disease (MCTD) was first recognized by Sharp and colleagues (1972) in a group of patients with ... Cyclooxygenase-2 (COX-2) inhibitors. Class Summary. Although increased cost can be a negative factor, COX-2 inhibitors may be ... encoded search term (Mixed%20Connective-Tissue%20Disease) and Mixed Connective-Tissue Disease What to Read Next on Medscape. ... Phosphodiesterase (type 5) Enzyme Inhibitor. Class Summary. Phosphodiesterase inhibitors can ameliorate symptoms of pulmonary ...
In this study, a series of Burkitts lymphoma (BL) cell lines were assayed for their expression of tissue inhibitor of ... In vitro suppression of programmed cell death of B cells by tissue inhibitor of metalloproteinases-1.. ... In vitro suppression of programmed cell death of B cells by tissue inhibitor of metalloproteinases-1.. ... These results demonstrate that TIMP-1 suppresses apoptosis in B cells and suggests a novel activity for TIMP-1 in tissue ...
Protease Inhibitors · Thiophenes · Thrombosis · Tissue Inhibitor of Metalloproteinase-1 · Tissue Inhibitor of Metalloproteinase ... Chemicals/CAS: marimastat, 154039-60-8; tissue inhibitor of metalloproteinase 1, 140208-24-8; tissue inhibitor of ... Tissue Inhibitor of Metalloproteinase-1 · Tissue Inhibitor of Metalloproteinase-3 · Transduction, Genetic · Adenoviridae · ... tissue inhibitor of metalloproteinase 3, 145809-21-8, 164781-40-2; Matrix Metalloproteinase 2, EC 3.4.24.24; Tissue Inhibitor ...
Blood sample was collected to analyze serum level of matrix metalloproteinase 3 (MMP3) and tissue inhibitor metalloproteinase 1 ... and tissue inhibitor metallo-proteinase 1 (TIMP1) in vascular dementia patients and healthy control subjects. Methods: A case ... Multiinfarct Dementia, Matrix Metalloproteinase 3, Tissue Inhibitor Me, Talloproteinase 1, Vascular Dementia, Vascular Dementia ... Aim: To compare serum level of matrix metalloproteinase 3 (MMP3) and tissue inhibitor metallo-proteinase 1 (TIMP1) in vascular ...
Tissue factor pathway inhibitor activity and APC resistance are related to CHD risk in women, but may not explain the increased ... Tissue Factor Pathway Inhibitor, Activated Protein C Resistance, and Risk of Coronary Heart Disease Due To Combined Estrogen ... Tissue factor pathway inhibitor, activated protein C resistance, and risk of coronary heart disease due to combined estrogen ... To examine whether tissue factor pathway inhibitor or acquired activated protein C (APC) resistance influences the increased ...
... and tctPA and little work on inhibition by the specific inhibitor plasminogen activator inhibitor-1 (PAI-1) under physiological ... C. Longstaff, C. Thelwell, S. C. Williams, M. M. C. G. Silva, L. Szabo, K. Kolev, The interplay between tissue plasminogen ... Background: Tissue plasminogen activator (tPA) is unusual in the coagulation and fibrinolysis cascades in that it is produced ... Arnaud Chevilley, Flavie Lesept, Sophie Lenoir, Carine Ali, Jérôme Parcq, Denis Vivien, Impacts of tissue-type plasminogen ...
... its tissue inhibitor (TIMP-2) and transforming growth factor beta 1 (TGF-β1) in the aqueous humor and serum samples of patients ... tissue inhibitor of matrix metalloproteinase-2, and transforming growth factor beta 1 in the aqueous humor and serum of ... and their tissue inhibitors (tissue inhibitors of matrix metalloproteinases [TIMPs]). This imbalance leads to accumulation of ... MMPs and their inhibitors have been previously shown in human aqueous humor and the surrounding tissues using zymographic and ...
... and their inhibitors (tissue inhibitor of metalloproteinases [TIMPs]), mainly secreted from activated macrophages.1 However, ... Adiponectin Specifically Increased Tissue Inhibitor of Metalloproteinase-1 Through Interleukin-10 Expression in Human ... Adiponectin Specifically Increased Tissue Inhibitor of Metalloproteinase-1 Through Interleukin-10 Expression in Human ... Adiponectin Specifically Increased Tissue Inhibitor of Metalloproteinase-1 Through Interleukin-10 Expression in Human ...
Thank you for submitting your article "Progenitors Oppositely Polarize WNT Activators and Inhibitors to Orchestrate Tissue ... For the porcupine inhibitor experiment, each half skin was treated with either porcupine inhibitor LGK974 (1 mM or 10 mM; ... by polarizing WNT-inhibitors apically in basal hair bud cells, a WNT-inhibitor free zone appeared to be generated at this ... Porcupine inhibitor washout experiment was performed by treating each half skin with 1 mM LGK974. After 12 hr one of the ...
Complex membrane type-1 matrix metalloproteinase (MT1-MMP) with tissue inhibitor of metalloproteinase-1 (TIMP-1). *DOI: 10.2210 ... A single point mutation at the distal site of the endogenous tissue inhibitor of metalloproteinase 1 (TIMP-1) enables this ... Metalloproteinase inhibitor 1. B, C. 125. Homo sapiens. Mutation(s): 3 Gene Names: TIMP1, CLGI, TIMP. ... The Intrinsic Protein Flexibility of Endogenous Protease Inhibitor TIMP-1 Controls Its Binding Interface and Affects Its ...
Murine tissue inhibitor of metalloproteinases-4 (Timp-4): cDNA isolation and expression in adult mouse tissues. FEBS Lett. 401 ... and their specific inhibitors, tissue inhibitors of metalloprotease (TIMP). ... Tissue inhibitor of metalloproteinases-1 promotes liver fibrosis development in a transgenic mouse model. Hepatology 32: 1248. ... Binding of tissue inhibitor of metalloproteinases 2 to two distinct sites on human 72-kDa gelatinase. Identification of a ...
  • Abstract 1019: Cytotoxicity in vitro, pharmacokinetics and tissue distribution of Link-N3 and Link-F3, two bivalent small molecules inhibitors of c-Myc/Max interactions in C.B-17 SCID mice bearing Daudi Burkitt's lymphoma xenografts. (aacrjournals.org)
  • Despite apparent differences, mineralized tissues are similarly derived by highly concerted extracellular processes involving matrix proteins, proteases, and mineral ion fluxes that collectively regulate the nucleation, growth and organization of forming mineral crystals. (frontiersin.org)
  • Although the mechanisms by which these mineralized tissues form are not fully understood, it is apparent that the unique structure of each mineralized tissue, including dental enamel, is the result of highly concerted cell and extracellular processes that regulate the on-set, growth rate, shape, location and arrangement of forming mineral crystals ( Weiner, 1986 ). (frontiersin.org)
  • Proteolytic remodeling of components of the extracellular matrix (ECM) in tissue interstitium is a prerequisite of the metastatic process ( 1 ). (aacrjournals.org)
  • BACKGROUND/AIMS Alterations in synthesis and breakdown of extracellular matrix components are known to play a crucial role in tissue remodelling during inflammation and wound healing. (bmj.com)
  • Chronic mucosal inflammation is characterised by an inflammatory cell infiltrate associated with changes in epithelial proliferation and migration, 1 paralleled by intensive remodelling of the subepithelial connective tissue leading to increased turnover of extracellular matrix (ECM) components. (bmj.com)
  • It is a 21-kDa nonglycosylated protein found in tissue fluid and is secreted as a complex with progelatinase A by human fibroblast and uncomplexed from alveolar macrophages. (labome.org)
  • Tissue Factor Pathway Inhibitor, Activated Protein C Resistance, and Risk of Coronary Heart Disease Due To Combined Estrogen Plus Progestin Therapy. (nih.gov)
  • To examine whether tissue factor pathway inhibitor or acquired activated protein C (APC) resistance influences the increased risk of coronary heart disease (CHD) due to estrogen plus progestin therapy. (nih.gov)
  • Adiponectin is an adipocyte-specific plasma protein that we identified in a human adipose tissue cDNA library. (ahajournals.org)
  • 7 Moreover, we recently found that C-reactive protein levels are negatively correlated with adiponectin levels in both human plasma and adipose tissue. (ahajournals.org)
  • Here we argue that controlling the intrinsic protein dynamics of MMP endogenous inhibitors may be utilized for rationalizing the design of selective novel protein inhibitors for this class of enzymes. (rcsb.org)
  • Although these tissues are similarly comprised of a crystalline calcium apatite mineral phase and a protein component, they differ with respect to crystal size and shape, level and distribution of trace mineral ions, the nature of the proteins present, and their relative proportions of mineral and protein components. (frontiersin.org)
  • We investigated acute effects of two allosteric protein kinase B (PKB) inhibitors, MK-2206 and Akti-1/2, on insulin-stimulated lipogenesis in rat epididymal adipocytes incubated with fructose as carbohydrate substrate. (portlandpress.com)
  • An increasing number of BET family protein inhibitors have recently entered clinical trials. (aacrjournals.org)
  • Occlusion of the left anterior descending coronary artery for 2 hours followed by 4 hours of reperfusion in anesthetized cats results in a large washout of creatine kinase into the blood (32 +/- 7 IU/mg protein) and an area of necrotic tissue comprising 52 +/- 5% of the area at risk and 9 +/- 0.6% of the left ventricle. (ahajournals.org)
  • Administration of the same doses of both t-PA and CGS-13080 together markedly attenuated creatine kinase release to 10 +/- 2 IU/mg protein (p less than 0.01) and reduced the area of necrotic tissue to 9 +/- 2% of the area at risk and only 1.3 +/- 0.3% of the left ventricle (p less than 0.001). (ahajournals.org)
  • Pohar, N., Godenschwege, T. A., and Buchner, E. (1999) Invertebrate tissue inhibitor of metalloproteinase: structure and nested gene organization within the synapsin locus is conserved from Drosophila to human. (springer.com)
  • Their similar biochemical activity in blocking CDK enzymes and maintaining the growth-suppressive activity of Rb predict a tumor suppression function for CDK inhibitor genes, yet only the p16 INK4a gene has been directly linked to tumor growth by genetic alterations found in human cancers ( 17 , 25 ) and by the early development of spontaneous tumors in mice lacking p16 ( 31 ). (asm.org)
  • Global gene expression profiling studies were carried out using cancer cells and surrogate tissues, such as whole blood and skin, to identify genes that are modulated by BET family proteins. (aacrjournals.org)
  • HEXIM1 was found to be the only gene that exhibited robust and consistent modulation by BET inhibitors across multiple cancer indications and surrogate tissues. (aacrjournals.org)
  • Bachman KE, Herman JG, Corn PG, Merlo A, Costello JF, Cavenee WK, Baylin SB, Graff JR Methylation-associated silencing of the tissue inhibitor ofmetalloproteinase-3 gene suggest a suppressor role in kidney, brain, andother human cancers. (urogene.org)
  • Vaspin cDNA was isolated by from visceral white adipose tissues (WATs) of Otsuka Long-Evans Tokushima fatty (OLETF) rat, an animal model of abdominal obesity with type 2 diabetes. (pnas.org)
  • Despite these important advances on the establishment of two-dimensional patterns, comparatively little is known about the molecular nature of the positional information needed to generate three-dimensional tissue patterns, or how closely juxtaposed cells within a developing tissue and organ adopt and maintain distinct cellular fates. (elifesciences.org)
  • Molecular-sieve chromatography of the inhibitor gives an Mr value for the inhibitor of 28 500. (biochemj.org)
  • Previous studies with high molecular weight thrombin inhibitors, such as hirudin, have shown variable improvement in the frequency of sustained thrombolysis. (aspetjournals.org)
  • This study was conducted to examine the modulation of thrombolysis, indices of thrombin generation and activated partial thromboplastin time (APTT) by a novel low molecular weight direct thrombin inhibitor, inogatran. (aspetjournals.org)
  • The ability to build asymmetry in molecular, cellular, and tissue structures is a fundamental property of development, growth, and regeneration and is essential for tissue function. (nature.com)
  • However, even though many of the molecular players required for barrier formation are shared between simple and multi-layered epithelia 2 , the mechanisms that drive this tissue polarity are unknown. (nature.com)
  • Notably, both the in vitro and in vivo efficacy of nintedanib was superior to that of imatinib, another multi-kinase inhibitor, previously tested with minimal success in clinical trials in sarcoma. (aacrjournals.org)
  • This study aimed to isolate normal tonsillar keratinocytes (NTK) from human tonsils, increase the lifespan of these cells using the Rho kinase inhibitor Y-27632 and to develop tissue-engineered equivalents of healthy and infected tonsil epithelium. (whiterose.ac.uk)
  • During the steady state of the U46619-induced contraction, Y27632 (10 μ M ), a Rho-kinase inhibitor, partially inhibited [Ca 2+ ] i , although it substantially inhibited tension and MLC phosphorylation. (wiley.com)
  • Wortmannin (10 μ M ), an MLC kinase inhibitor, had no significant effect on [Ca 2+ ] i , but it completely inhibited MLC phosphorylation and partially inhibited tension. (wiley.com)
  • Apoptosis, the programmed cell death, is critical for the development and maintenance of healthy tissues. (aacrjournals.org)
  • These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. (gbiosciences.com)
  • Incorporating well-characterized pharmacodynamic markers, such as HEXIM1 and other genes described here, can provide a better understanding of potential efficacy and toxicity associated with inhibiting BET family proteins and informs early clinical decisions on BET inhibitor development programs. (aacrjournals.org)
  • GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin), Human, ELISA Kit. (antibody-antibodies.com)
  • Biomineralization is the process by a wide variety of living organisms, including mollusks, sponges and unicellular diatoms, for example, produce functional mineralized tissues ( Mann, 2001 ). (frontiersin.org)
  • Our results suggest that functional collaborations between distinct CDK inhibitor genes are tissue specific and confer yet another level of regulation in cell growth control and tumor suppression. (asm.org)
  • Early treatment following an acute stroke with the thrombolytic tissue plasminogen activator (tPA) increases functional recovery. (ovid.com)
  • The goal of the current study was to test the effect of a combination of argatroban, a thrombin inhibitor, and tPA on functional recovery and brain infarction size following thromboembolic ischemia in nonhuman primates. (ovid.com)
  • However, in contrast to previous findings in rats, the combination of argatroban and tPA does not appear to further enhance tissue or functional recovery from a thromboembolic stroke compared to tPA treatment alone. (ovid.com)
  • This functional tissue-engineered equivalent of tonsil epithelium will provide a valuable tool for studying tonsil biology and host-pathogen interactions in a more physiologically relevant manner. (whiterose.ac.uk)
  • Phosphodiesterase inhibitors can ameliorate symptoms of pulmonary hypertension and Raynaud phenomenon in patients with MCTD. (medscape.com)
  • Metastasis and progressive scattering of tumor cells throughout tissues is the main cause of organ failure and subsequent death of cancer patients. (aacrjournals.org)
  • With further testing and development, such a compound could lead to therapies that would help patients heal tissues damaged by disease or excised through surgery. (acs.org)
  • In previous studies on animals or patients, scientists have tried to accelerate tissue and blood cell growth by administering analogs of prostaglandin E 2 (PGE 2 ), a lipid that promotes regeneration by initiating signals that cause stem cells to proliferate. (acs.org)
  • Hematologist Leonard I. Zon of Harvard Medical School, whose group played a leading part in discovering PGE 2 's natural role in tissue regeneration, comments that when prostaglandins are given to patients directly, they can degrade quickly in the bloodstream, diminishing the effectiveness of the therapy. (acs.org)
  • It has been reported that attempts of monitoring target engagement of the BET bromodomain inhibitor OTX015 using literature-described putative pharmacodynamic markers, such as c-Myc, BRD2 , etc., failed to detect pharmacodynamic marker responses in AML patients treated at active dose and those with clinical responses. (aacrjournals.org)
  • The research team aimed to develop an inactive TKI that activates selectively in malignant tissue only, thereby preventing damage to healthy tissue and minimizing side effects in patients. (oncologynurseadvisor.com)
  • Tissue inhibitor of metalloproteinase 4 in aqueous humor of patients with primary open angle glaucoma, pseudoexfoliation syndrome and pseudoexfoliative glaucoma and its role in proteolysis imbalance. (ebscohost.com)
  • AM from 13 chronic obstructive pulmonary disease (COPD) patients and 10 smoking controls and lung tissue from 7 COPD patients were stimulated with LPS following preincubation with roflumilast (0.000001-10 µM), CHF6001 (0.000001-0.1 µM), or vehicle. (manchester.ac.uk)
  • PDE4 A, B and D expression were increased in alveolar macrophages and lung tissue of COPD patients compared to controls. (manchester.ac.uk)
  • Information, treatment algorithms and educational materials for healthcare professionals and patients relating to the management of dermatological toxicities in patients treated with multikinase inhibitors. (esmo.org)
  • Methods: Tissue samples from the lower uterine segment were obtained from 32 patients undergoing secondary cesarean delivery of a singleton infant before term. (uni-bielefeld.de)
  • Previously, we isolated a ≈135-bp cDNA fragment during differential screening of the genes up-regulated in visceral adipose tissues of obese OLETF rats and down-regulated in nonobese and diabetes-resistant Long-Evans Tokushima Otsuka (LETO) rats ( 22 ). (pnas.org)
  • The presence of two families of seven distinct mammalian cyclin-dependent kinase (CDK) inhibitor genes is thought to mediate the complexity of connecting a variety of cellular processes to the cell cycle control pathway. (asm.org)
  • The distinct pattern of tissue expression of CDK inhibitor genes suggests that they may function as tumor suppressors with different tissue specificities. (asm.org)
  • Conceptually, genes that negatively regulate the growth-suppressing activity of either p53 or pRb may be proto-oncogenes, as exemplified by the observation that MDM2 ( 26 ) and cyclin D1 ( 20 ), negative regulators of p53 and pRb, respectively, are frequently activated in human cancers and promote tumor growth when targeted for transgenic expression in mouse mammary tissues ( 22 , 35 ). (asm.org)
  • Two families of cyclin-dependent kinase (CDK) inhibitors, totaling seven genes, have been identified in mammalian cells. (asm.org)
  • Neither mutational analysis in human tumors nor phenotypic examination of genetically targeted mice lacking any of the other individual CDK inhibitor genes has provided strong evidence for a direct role for any of the other CDK inhibitors as tumor suppressors. (asm.org)
  • Here, we report the identification and characterization of HEXIM1 and other genes as robust pharmacodynamic markers for BET inhibitors. (aacrjournals.org)
  • EGLN inhibitor protects normal tissue and enables RT in mouse model of pancreatic cancer. (ecancer.org)
  • Administration of the EGLN inhibitor FG-4592 prior to ablative radiotherapy provided protection against fatal gastrointestinal bleeding and improved survival in a mouse model of unresectable pancreatic cancer according to a new study posted in Cancer Research , a journal of the American Association for Cancer Research. (ecancer.org)
  • We identified an adipocytokine, designated as visceral adipose tissue-derived serpin (vaspin), which is a member of serine protease inhibitor family. (pnas.org)
  • In this study, we describe the isolation of the full-length cDNA of this potential inhibitor, designated as "vaspin" (visceral adipose tissue-derived serpin). (pnas.org)
  • Adipose tissue secretes various bioactive molecules, termed adipocytokines, that directly contribute to obesity-linked metabolic and vascular diseases. (ahajournals.org)
  • ACL and ACC purified from white adipose tissue were poor substrates for PKBa in vitro . (portlandpress.com)
  • Freshly collected visceral adipose tissue did not exhibit a higher content of PAI-1 or TGF-beta1 than did SC tissue. (diabetesjournals.org)
  • CONCLUSION Profound overexpression of MMP-1 and MMP-3 mRNA transcripts suggests an important role for these enzymes in the process of tissue remodelling and destruction in inflammatory bowel disease. (bmj.com)
  • Nintedanib, a potent triple angiokinase inhibitor, targets PDGFR, VEGFR and FGFR pathways critical for tumor angiogenesis and vasculature. (aacrjournals.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • These data indicate that MMP-1, MMP-2 and MMP-9 are highly expressed in human breast carcinoma tissue and suggest that activation of proMMP-2 may be an indicator of lymphnode metastasis of the breast carcinoma. (nih.gov)
  • Six of these hyperplastic tissues and tumors were in endocrine organs, and several types of tumors routinely developed within the same animal, a phenotype reminiscent of that seen in combined human multiple endocrine neoplasia syndromes. (asm.org)
  • Pseudoexfoliation (PEX) syndrome is a systemic disease characterized by excessive synthesis and progressive accumulation of a fibrillar material in various tissues, including the eye. (dovepress.com)