Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Application of principles and practices of engineering science to biomedical research and health care.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)
Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.
Artificial organs that are composites of biomaterials and cells. The biomaterial can act as a membrane (container) as in BIOARTIFICIAL LIVER or a scaffold as in bioartificial skin.
The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.
A field of medicine concerned with developing and using strategies aimed at repair or replacement of damaged, diseased, or metabolically deficient organs, tissues, and cells via TISSUE ENGINEERING; CELL TRANSPLANTATION; and ARTIFICIAL ORGANS and BIOARTIFICIAL ORGANS and tissues.
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
Submicron-sized fibers with diameters typically between 50 and 500 nanometers. The very small dimension of these fibers can generate a high surface area to volume ratio, which makes them potential candidates for various biomedical and other applications.
Procedures for enhancing and directing tissue repair and renewal processes, such as BONE REGENERATION; NERVE REGENERATION; etc. They involve surgically implanting growth conducive tracks or conduits (TISSUE SCAFFOLDING) at the damaged site to stimulate and control the location of cell repopulation. The tracks or conduits are made from synthetic and/or natural materials and may include support cells and induction factors for CELL GROWTH PROCESSES; or CELL MIGRATION.
Renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone.
Materials fabricated by BIOMIMETICS techniques, i.e., based on natural processes found in biological systems.
A network of cross-linked hydrophilic macromolecules used in biomedical applications.
Methods and techniques used to genetically modify cells' biosynthetic product output and develop conditions for growing the cells as BIOREACTORS.
Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Salts and esters of the 10-carbon monocarboxylic acid-decanoic acid.
Implants constructed of materials designed to be absorbed by the body without producing an immune response. They are usually composed of plastics and are frequently used in orthopedics and orthodontics.
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
Methods for maintaining or growing CELLS in vitro.
The application of engineering principles and methods to living organisms or biological systems.
A biocompatible polymer used as a surgical suture material.
The process of bone formation. Histogenesis of bone including ossification.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
A continuous protein fiber consisting primarily of FIBROINS. It is synthesized by a variety of INSECTS and ARACHNIDS.
A generic term for all substances having the properties of stretching under tension, high tensile strength, retracting rapidly, and recovering their original dimensions fully. They are generally POLYMERS.
Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.
Manufacturing technology for making microscopic devices in the micrometer range (typically 1-100 micrometers), such as integrated circuits or MEMS. The process usually involves replication and parallel fabrication of hundreds or millions of identical structures using various thin film deposition techniques and carried out in environmentally-controlled clean rooms.
The maximum compression a material can withstand without failure. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed, p427)
The physiological renewal, repair, or replacement of tissue.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.
The properties and processes of materials that affect their behavior under force.
Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.
Artificial substitutes for body parts and materials inserted into organisms during experimental studies.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
An interdisciplinary field in materials science, ENGINEERING, and BIOLOGY, studying the use of biological principles for synthesis or fabrication of BIOMIMETIC MATERIALS.
Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.
Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.
Polymorphic cells that form cartilage.
The maximum stress a material subjected to a stretching load can withstand without tearing. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed, p2001)
A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.
Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.
Synthetic or natural materials for the replacement of bones or bone tissue. They include hard tissue replacement polymers, natural coral, hydroxyapatite, beta-tricalcium phosphate, and various other biomaterials. The bone substitutes as inert materials can be incorporated into surrounding tissue or gradually replaced by original tissue.
A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Characteristics or attributes of the outer boundaries of objects, including molecules.
Fibrous proteins secreted by INSECTS and SPIDERS. Generally, the term refers to silkworm fibroin secreted by the silk gland cells of SILKWORMS, Bombyx mori. Spider fibroins are called spidroins or dragline silk fibroins.
The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.
A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)
Nanometer-scale composite structures composed of organic molecules intimately incorporated with inorganic molecules. (Glossary of Biotechnology and Nanobiotechology Terms, 4th ed)
A normal intermediate in the fermentation (oxidation, metabolism) of sugar. The concentrated form is used internally to prevent gastrointestinal fermentation. (From Stedman, 26th ed)
Methods and techniques used to modify or select cells and develop conditions for growing cells for biosynthetic production of molecules (METABOLIC ENGINEERING), for generation of tissue structures and organs in vitro (TISSUE ENGINEERING), or for other BIOENGINEERING research objectives.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Numerical expression indicating the measure of stiffness in a material. It is defined by the ratio of stress in a unit area of substance to the resulting deformation (strain). This allows the behavior of a material under load (such as bone) to be calculated.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
A group of thermoplastic or thermosetting polymers containing polyisocyanate. They are used as ELASTOMERS, as coatings, as fibers and as foams.
Synthetic material used for the treatment of burns and other conditions involving large-scale loss of skin. It often consists of an outer (epidermal) layer of silicone and an inner (dermal) layer of collagen and chondroitin 6-sulfate. The dermal layer elicits new growth and vascular invasion and the outer layer is later removed and replaced by a graft.
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
The quality of surface form or outline of CELLS.
The fibrous CONNECTIVE TISSUE surrounding the TOOTH ROOT, separating it from and attaching it to the alveolar bone (ALVEOLAR PROCESS).
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
A potent osteoinductive protein that plays a critical role in the differentiation of osteoprogenitor cells into OSTEOBLASTS.
The properties, processes, and behavior of biological systems under the action of mechanical forces.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
A type of CARTILAGE whose matrix contains large bundles of COLLAGEN TYPE I. Fibrocartilage is typically found in the INTERVERTEBRAL DISK; PUBIC SYMPHYSIS; TIBIAL MENISCI; and articular disks in synovial JOINTS. (From Ross et. al., Histology, 3rd ed., p132,136)
Products made by baking or firing nonmetallic minerals (clay and similar materials). In making dental restorations or parts of restorations the material is fused porcelain. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed & Boucher's Clinical Dental Terminology, 4th ed)
Cells with high proliferative and self renewal capacities derived from adults.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Adherence of cells to surfaces or to other cells.
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Flaps of tissue that prevent regurgitation of BLOOD from the HEART VENTRICLES to the HEART ATRIA or from the PULMONARY ARTERIES or AORTA to the ventricles.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
Operative procedures performed on the SKIN.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC
Transference of cells within an individual, between individuals of the same species, or between individuals of different species.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS.
The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
A polyester used for absorbable sutures & surgical mesh, especially in ophthalmic surgery. 2-Hydroxy-propanoic acid polymer with polymerized hydroxyacetic acid, which forms 3,6-dimethyl-1,4-dioxane-dione polymer with 1,4-dioxane-2,5-dione copolymer of molecular weight about 80,000 daltons.
The branch of medicine concerned with the application of NANOTECHNOLOGY to the prevention and treatment of disease. It involves the monitoring, repair, construction, and control of human biological systems at the molecular level, using engineered nanodevices and NANOSTRUCTURES. (From Freitas Jr., Nanomedicine, vol 1, 1999).
A type of CARTILAGE characterized by a homogenous amorphous matrix containing predominately TYPE II COLLAGEN and ground substance. Hyaline cartilage is found in ARTICULAR CARTILAGE; COSTAL CARTILAGE; LARYNGEAL CARTILAGES; and the NASAL SEPTUM.
Microbial, plant, or animal cells which are immobilized by attachment to solid structures, usually a column matrix. A common use of immobilized cells is in biotechnology for the bioconversion of a substrate to a particular product. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The flexible rope-like structure that connects a developing FETUS to the PLACENTA in mammals. The cord contains blood vessels which carry oxygen and nutrients from the mother to the fetus and waste products away from the fetus.
Device constructed of either synthetic or biological material that is used for the repair of injured or diseased blood vessels.
Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).
Prosthesis, usually heart valve, composed of biological material and whose durability depends upon the stability of the material after pretreatment, rather than regeneration by host cell ingrowth. Durability is achieved 1, mechanically by the interposition of a cloth, usually polytetrafluoroethylene, between the host and the graft, and 2, chemically by stabilization of the tissue by intermolecular linking, usually with glutaraldehyde, after removal of antigenic components, or the use of reconstituted and restructured biopolymers.
Mesodermal tissue enclosed in the invaginated portion of the epithelial enamel organ and giving rise to the dentin and pulp.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
The structures surrounding and supporting the tooth. Periodontium includes the gum (GINGIVA), the alveolar bone (ALVEOLAR PROCESS), the DENTAL CEMENTUM, and the PERIODONTAL LIGAMENT.
A fibrillar collagen found predominantly in CARTILAGE and vitreous humor. It consists of three identical alpha1(II) chains.
A protein derived from FIBRINOGEN in the presence of THROMBIN, which forms part of the blood clot.
The interarticular fibrocartilages of the superior surface of the tibia.
Fibrous bands or cords of CONNECTIVE TISSUE at the ends of SKELETAL MUSCLE FIBERS that serve to attach the MUSCLES to bones and other structures.
Techniques for enhancing and directing cell growth to repopulate specific parts of the PERIODONTIUM that have been damaged by PERIODONTAL DISEASES; TOOTH DISEASES; or TRAUMA, or to correct TOOTH ABNORMALITIES. Repopulation and repair is achieved by guiding the progenitor cells to reproduce in the desired location by blocking contact with surrounding tissue by use of membranes composed of synthetic or natural material that may include growth inducing factors as well.
Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.
Thin outer membrane that surrounds a bone. It contains CONNECTIVE TISSUE, CAPILLARIES, nerves, and a number of cell types.
Methods of creating machines and devices.
Restoration of integrity to traumatized tissue.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
Cartilage of the EAR AURICLE and the EXTERNAL EAR CANAL.
Artificial substitutes for body parts, and materials inserted into tissue for functional, cosmetic, or therapeutic purposes. Prostheses can be functional, as in the case of artificial arms and legs, or cosmetic, as in the case of an artificial eye. Implants, all surgically inserted or grafted into the body, tend to be used therapeutically. IMPLANTS, EXPERIMENTAL is available for those used experimentally.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
The bonelike rigid connective tissue covering the root of a tooth from the cementoenamel junction to the apex and lining the apex of the root canal, also assisting in tooth support by serving as attachment structures for the periodontal ligament. (Jablonski, Dictionary of Dentistry, 1992)
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
A group of phosphate minerals that includes ten mineral species and has the general formula X5(YO4)3Z, where X is usually calcium or lead, Y is phosphorus or arsenic, and Z is chlorine, fluorine, or OH-. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
The MUSCLES, bones (BONE AND BONES), and CARTILAGE of the body.
Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.
X-RAY COMPUTERIZED TOMOGRAPHY with resolution in the micrometer range.
The use of computers for designing and/or manufacturing of anything, including drugs, surgical procedures, orthotics, and prosthetics.
Propylene or propene polymers. Thermoplastics that can be extruded into fibers, films or solid forms. They are used as a copolymer in plastics, especially polyethylene. The fibers are used for fabrics, filters and surgical sutures.
The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Therapies that involve the TRANSPLANTATION of CELLS or TISSUES developed for the purpose of restoring the function of diseased or dysfunctional cells or tissues.
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Any of the 23 plates of fibrocartilage found between the bodies of adjacent VERTEBRAE.
A type of MONOTERPENES, derived from geraniol. They have the general form of cyclopentanopyran, but in some cases, one of the rings is broken as in the case of secoiridoid. They are different from the similarly named iridals (TRITERPENES).
Loose connective tissue lying under the DERMIS, which binds SKIN loosely to subjacent tissues. It may contain a pad of ADIPOCYTES, which vary in number according to the area of the body and vary in size according to the nutritional state.
Macroporous hydrogels that are produced at subzero temperatures. Cryogels have pores that are produced by growing ice crystals and have been developed with a tissue-like elasticity that is suitable for cell immunization experiments.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Resistance and recovery from distortion of shape.
The behaviors of materials under force.
A TGF-beta subtype that plays role in regulating epithelial-mesenchymal interaction during embryonic development. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta3 and TGF-beta3 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor.
Cellular functions, mechanisms, and activities.
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
A field of biological research combining engineering in the formulation, design, and building (synthesis) of novel biological structures, functions, and systems.
The SKELETON of the HEAD including the FACIAL BONES and the bones enclosing the BRAIN.
The study of the deformation and flow of matter, usually liquids or fluids, and of the plastic flow of solids. The concept covers consistency, dilatancy, liquefaction, resistance to flow, shearing, thixotrophy, and VISCOSITY.
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from
The profession concerned with the teeth, oral cavity, and associated structures, and the diagnosis and treatment of their diseases including prevention and the restoration of defective and missing tissue.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices.
The branch of surgery concerned with restoration, reconstruction, or improvement of defective, damaged, or missing structures.
A biocompatible, hydrophilic, inert gel that is permeable to tissue fluids. It is used as an embedding medium for microscopy, as a coating for implants and prostheses, for contact lenses, as microspheres in adsorption research, etc.
The differentiation of pre-adipocytes into mature ADIPOCYTES.
The application of discoveries generated by laboratory research and preclinical studies to the development of clinical trials and studies in humans. A second area of translational research concerns enhancing the adoption of best practices.
Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.
The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues.
An articulation between the condyle of the mandible and the articular tubercle of the temporal bone.
Non-striated, elongated, spindle-shaped cells found lining the digestive tract, uterus, and blood vessels. They are derived from specialized myoblasts (MYOBLASTS, SMOOTH MUSCLE).
A family of structurally related collagens that form the characteristic collagen fibril bundles seen in CONNECTIVE TISSUE.
Compounds based on fumaric acid.
A subclass of iridoid compounds that include a glycoside moiety, usually found at the C-1 position.
Colorless, odorless crystals that are used extensively in research laboratories for the preparation of polyacrylamide gels for electrophoresis and in organic synthesis, and polymerization. Some of its polymers are used in sewage and wastewater treatment, permanent press fabrics, and as soil conditioning agents.
Procedures that stimulate nerve elongation over a period of time. They are used in repairing nerve tissue.
A mutant strain of Rattus norvegicus without a thymus and with depressed or absent T-cell function. This strain of rats may have a small amount of hair at times, but then lose it.
Transference of tissue within an individual, between individuals of the same species, or between individuals of different species.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Nanometer-sized tubes composed of various substances including carbon (CARBON NANOTUBES), boron nitride, or nickel vanadate.
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
Procedures used to reconstruct, restore, or improve defective, damaged, or missing structures.
Biocompatible materials usually used in dental and bone implants that enhance biologic fixation, thereby increasing the bond strength between the coated material and bone, and minimize possible biological effects that may result from the implant itself.
Dense fibrous layer formed from mesodermal tissue that surrounds the epithelial enamel organ. The cells eventually migrate to the external surface of the newly formed root dentin and give rise to the cementoblasts that deposit cementum on the developing root, fibroblasts of the developing periodontal ligament, and osteoblasts of the developing alveolar bone.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
The process by which cells convert mechanical stimuli into a chemical response. It can occur in both cells specialized for sensing mechanical cues such as MECHANORECEPTORS, and in parenchymal cells whose primary function is not mechanosensory.
The ability to recognize when information is needed and to locate, evaluate, and use the needed information effectively.
Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.
Endothelial cells that line venous vessels of the UMBILICAL CORD.
Differentiated tissue of the central nervous system composed of NERVE CELLS, fibers, DENDRITES, and specialized supporting cells.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
Degenerative changes in the INTERVERTEBRAL DISC due to aging or structural damage, especially to the vertebral end-plates.
One of a set of bone-like structures in the mouth used for biting and chewing.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Relating to the size of solids.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
The fern plant family of the order Polypodiales, class Filicopsida, division Pteridophyta, subkingdom Tracheobionta.
A negatively-charged extracellular matrix protein that plays a role in the regulation of BONE metabolism and a variety of other biological functions. Cell signaling by osteopontin may occur through a cell adhesion sequence that recognizes INTEGRIN ALPHA-V BETA-3.
Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from:
Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
Devices intended to replace non-functioning organs. They may be temporary or permanent. Since they are intended always to function as the natural organs they are replacing, they should be differentiated from PROSTHESES AND IMPLANTS and specific types of prostheses which, though also replacements for body parts, are frequently cosmetic (EYE, ARTIFICIAL) as well as functional (ARTIFICIAL LIMBS).
Acrylic acids or acrylates which are substituted in the C-2 position with a methyl group.
Devices for simulating the activities of the liver. They often consist of a hybrid between both biological and artificial materials.
Nanometer-sized tubes composed mainly of CARBON. Such nanotubes are used as probes for high-resolution structural and chemical imaging of biomolecules with ATOMIC FORCE MICROSCOPY.
A species of SWINE, in the family Suidae, comprising a number of subspecies including the domestic pig Sus scrofa domestica.
Organic compounds that contain silicon as an integral part of the molecule.
Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.
Striated muscle cells found in the heart. They are derived from cardiac myoblasts (MYOBLASTS, CARDIAC).
Chemical reactions effected by light.
Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.
Chemical reaction in which monomeric components are combined to form POLYMERS (e.g., POLYMETHYLMETHACRYLATE).
Derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a carboxy terminated six carbon aliphatic structure.

Born again bone: tissue engineering for bone repair. (1/3513)

Destruction of bone tissue due to disease and inefficient bone healing after traumatic injury may be addressed by tissue engineering techniques. Growth factor, cytokine protein, and gene therapies will be developed, which, in conjunction with suitable carriers, will regenerate missing bone or help in cases of defective healing.  (+info)

Engineering virtual cardiac tissue. (2/3513)

The kinetics of proteins involved in ion transfer, sequestration and binding in cardiac cells can be modelled to construct a model of the electrical activity of isolated cardiac cells as a system of ordinary differential equations. These cell models may be incorporated into tissue models, which, when combined with histology and anatomy, form virtual tissues. The effects of changes in specific protein expression, or changes in protein kinetics, produced by mutations or pharmacological agents, can be simulated using these tissue models and used to account for the whole organ effects of changes in specific ion-transport protein activity.  (+info)

Cellular integration of thyrocytes and thyroid folliculogenesis: a perspective for thyroid tissue regeneration and engineering. (3/3513)

Thyroid gland is composed of many spheroid structures called thyroid follicles, in which thyrocytes are integrated in their specific structural and functional polarization. In conventional monolayer and floating cultures, the cells cannot reorganize follicle structures with normal polarity. By contrast, in a 3-D collagen gel culture thyrocytes easily and stably reconstruct follicles with physiological polarity. Integration of thyrocyte growth and differentiation appears to result in eventual thyroid folliculogenesis. 3-D collagen gel culture and subacute thyroiditis, a specific thyroid disorder, are the promising models for addressing the mechanism of thyroid folliculogenesis. Because formation of 3-D follicles actively occurs both in this culture system and at the regenerative stage of the disease. The understanding of the mechanistic basis of folliculogenesis is prerequisite for establishment of an artificial thyroid tissue, which would enable a more physiological approach to the treatment of hypothyroidism caused by various diseases and surgical processes than conventional hormone replacement therapy. In this review, we have discussed thyrocyte integration, and thyroid folliculogenesis and tissue regeneration, to further thyroid biology. Also, we briefly discussed a perspective on thyroid tissue regeneration and engineering.  (+info)

Oxygen diffusion and consumption of aortic valve cusps. (4/3513)

To maintain tissue oxygenation, normal aortic valves contain a vascular bed where tissue thickness is greatest. Avascular "living" tissue-engineered heart valves have been proposed, yet little information exists regarding the magnitude of valve tissue metabolic activity or oxygen requirements. We therefore set out to measure the oxygen diffusivity (DO(2)) and oxygen consumption (VO(2)) of seven porcine aortic valve cusps in vitro at 37 degrees C using a chamber with a Clark oxygen sensor. Mean DO(2) and VO(2) were 1.06 x 10(-5) cm(2)/s and 3.05 x 10(-5) x ml O(2). ml tissue(-1) x s(-1), respectively. When modeled as a three-layered structure by using these values and a boundary condition of 100 mmHg at both surfaces, the average aortic cusp predicted a central mean PO(2) of 27 mmHg (range of 0-50 mmHg). The DO(2) value obtained was similar to that found for other vascular structures, but because our studies were carried out in vitro, the VO(2) measurements may be lower than that required by the functioning valves. These values provide an initial understanding of the oxygen supply possible from the cusp surfaces and the oxygen needs of the tissue.  (+info)

Self-assembly and mineralization of peptide-amphiphile nanofibers. (5/3513)

We have used the pH-induced self-assembly of a peptide-amphiphile to make a nanostructured fibrous scaffold reminiscent of extracellular matrix. The design of this peptide-amphiphile allows the nanofibers to be reversibly cross-linked to enhance or decrease their structural integrity. After cross-linking, the fibers are able to direct mineralization of hydroxyapatite to form a composite material in which the crystallographic c axes of hydroxyapatite are aligned with the long axes of the fibers. This alignment is the same as that observed between collagen fibrils and hydroxyapatite crystals in bone.  (+info)

Contaminants from the transplant contribute to intimal hyperplasia associated with microvascular endothelial cell seeding. (6/3513)

OBJECTIVES: seeding prosthetic grafts with fat-derived microvascular endothelial cells (MVEC) results not only in a non-thrombogenic EC layer, but also in intimal hyperplasia. Here we investigated incidence, composition, progression, and cause of this intimal hyperplasia. DESIGN: EPTFE grafts with MVEC were implanted as carotid interpositions in six dogs with 1 month, and in three dogs with 4, 8 and 12 months follow-up. Grafts seeded without cells, implanted in the contralateral carotid, served as a control. In another three dogs labelled cells were seeded to investigate the contribution of the seeded cells (2-3 weeks). MATERIALS AND METHODS: MVEC were isolated from the falciform ligament. Cells were pressure seeded on ePTFE grafts. Labelling was performed using retroviral gene transduction. The grafts were analysed with immunohistochemical techniques. RESULTS: after 1 month, all patent non-seeded grafts (5/6) showed fibrin and platelet deposition, and all patent seeded grafts (5/6) were covered with a confluent endothelial monolayer on top of a multilayer of myofibroblasts, elastin and collagen. After long term follow-up, all non-seeded grafts were occluded, all patent seeded grafts (4 and 12 months) were covered with an EC-layer with intimal hyperplasia underneath. The thickness of the intima did not progress after 1 month. Transduced cells were found in the endothelial monolayer, hyperplastic intima and luminal part of the prosthesis. CONCLUSIONS: MVEC seeding in dogs results in intimal hyperplasia in all patent grafts, which contains myofibroblasts. Contaminants from the transplant contribute to this intimal hyperplasia.  (+info)

Replacing and renewing: synthetic materials, biomimetics, and tissue engineering in implant dentistry. (7/3513)

Hundreds of thousands of implantations are performed each year in dental clinical practice. Dental implants are a small fraction of the total number of synthetic materials implanted into the human body in all fields of medicine. Basically, these millions of implants going into humans function adequately. But longevity and complications still are significant issues and provide opportunities for the creation of improved devices. This manuscript briefly reviews the history of dental implant devices and the concepts surrounding the word "biocompatibility." It then contrasts the foreign body reaction with normal healing. Finally, the article describes how ideas gleaned from the study of normal wound healing can be applied to improved dental implants. In a concluding section, three scenarios for dental implants twenty years from now are envisioned.  (+info)

Expression of renal cell protein markers is dependent on initial mechanical culture conditions. (8/3513)

The rotating wall vessel is optimized for suspension culture, with laminar flow and adequate nutrient delivery, but minimal shear. However, higher shears may occur in vivo. During rotating wall vessel cultivation of human renal cells, size and density of glass-coated microcarrier beads were changed to modulate initial shear. Renal-specific proteins were assayed after 2 days. Flow cytometry antibody binding analysis of vitamin D receptor demonstrated peak expression at intermediate shears, with 30% reduction outside this range. Activity of cathepsin C showed the inverse pattern, lowest at midshear, with twofold increases at either extreme. Dipeptidyl-peptidase IV had no shear dependence, suggesting that the other results are specific, not universal, changes in membrane trafficking or protein synthesis. On addition of dextran, which changes medium density and viscosity but not shear, vitamin D receptor assay showed no differences from controls. Neither cell cycle, apoptosis/necrosis indexes, nor lactate dehydrogenase release varied between experiments, confirming that the changes are primary, not secondary to cell cycling or membrane damage. This study provides direct evidence that mechanical culture conditions modulate protein expression in suspension culture.  (+info)

TY - JOUR. T1 - Hydrostatic pressure in articular cartilage tissue engineering. T2 - From chondrocytes to tissue regeneration. AU - Elder, Benjamin D.. AU - Athanasiou, Kyriacos A.. PY - 2009/3/1. Y1 - 2009/3/1. N2 - Cartilage has a poor intrinsic healing response, and neither the innate healing response nor current clinical treatments can restore its function. Therefore, articular cartilage tissue engineering is a promising approach for the regeneration of damaged tissue. Because cartilage is exposed to mechanical forces during joint loading, many tissue engineering strategies use exogenous stimuli to enhance the biochemical or biomechanical properties of the engineered tissue. Hydrostatic pressure (HP) is emerging as arguably one of the most important mechanical stimuli for cartilage, although no optimal treatment has been established across all culture systems. Therefore, this review evaluates prior studies on articular cartilage involving the use of HP, with a particular emphasis on the ...
TY - JOUR. T1 - Functional tissue engineering : ten more years of progress. AU - Guilak, F.. AU - Baaijens, F.P.T.. PY - 2014. Y1 - 2014. N2 - Functional tissue engineering is a subset of the field of tissue engineering that was proposed by the United States National Committee on Biomechanics over a decade ago in order to place more emphasis on the roles of biomechanics and mechanobiology in tissue repair and regeneration. Over the past decade, there have been tremendous advances in this area, pointing out the critical role that biomechanical factors can play in the engineered repair of virtually all tissue and organ systems. In this special issue of the Journal of Biomechanics, we present a series of articles that address a broad array of the fundamental topics of functional tissue engineering, including: (1) measurement and modeling of the in vivo biomechanical environment and history in native and repair tissues; (2) further understanding of the biomechanical properties of native tissues ...
Engineering technology is that part of the technological field which requires the application of scientific and engineering knowledge and methods, combined with technical skills, for the implementation and extension of existing technologies. Engineering technology education focuses on preparing engineering technologists for positions that involve product development and improvement, system development, management, manufacturing and engineering operational functions. Graduates also enter the technical sales and customer services field, or continue in graduate work in engineering or management. Placement of graduates has been excellent.. The Engineering Technology Program awards Bachelor of Science in Engineering Technology (BSET) degrees for each of the following degree options: Construction Engineering Technology (CET), Electrical and Computer Engineering Technology (ECET), Mechanical Engineering Technology (MET), Medical Informatics Technology (MIT), Surveying Engineering Technology (SET), and ...
A key factor in the tissue engineering approach to tissue repair and regeneration is the use of appropriate cells. Mesenchymal stem cells (MSCs) are derived from bone marrow stroma or connective tissues and they have the potential to differentiate into various mesenchymal cell lines in vitro and in vivo. These cells hold great promise for musculoskeletal tissue engineering. This review is based mainly on the work which has been done in the National University of Singapore on the use of MSCs for engineering cartilage, growth plate, bone and tendon/ligament as well as the clinical trail of autologous chondrocyte implantation. It can help to shape future research on musculosketetal tissue engineering ...
The ability to heal soft tissue injuries and regenerate cartilage is the Holy Grail of musculoskeletal medicine. Articular cartilage repair and regeneration is considered to be largely intractable due to the poor regenerative properties of this tissue. Due to their low self-repair ability, cartilage defects that result from joint injury, aging, or osteoarthritis, are the most often irreversible and are a major cause of joint pain and chronic disability. However, current methods do not perfectly restore hyaline cartilage and may lead to the apparition of fibro- or continue hypertrophic cartilage. The lack of efficient modalities of treatment has prompted research into tissue engineering combining stem cells, scaffold materials and environmental factors. The field of articular cartilage tissue engineering, which aims to repair, regenerate, and/or improve injured or diseased cartilage functionality, has evoked intense interest and holds great potential for improving cartilage therapy. Plasma-rich ...
Biomaterials †Khademhosseini Laboratory. The Journal mainly promotes the novel emerging Biomaterial applications to Medical Sciences like Biomaterials, Tissue Engineering Biomaterials Applications,, Buy Biomaterials for Tissue Engineering Applications from Dymocks online BookStore. Find latest reader reviews and much more at Dymocks. Biomaterials and Tissue Engineering MSc UCL Mechanical Engineering. Application and next steps. Applications. Students are advised Application fee: Biomaterials and scaffolds for tissue engineering OBrien F. Biomaterials and scaffolds for tissue the art of scaffolds for tissue engineering applications.. With advancements in biological and engineering sciences, the definition of an ideal biomaterial has evolved over the past 50 years from a substance that is inert to Enhancing cell penetration and proliferation in chitosan hydrogels for tissue engineering applications Chengdong Jia, Ali Khademhosseinib,c,d, Fariba Dehghania,*. Interdisciplinary research into ...
[email protected] Engineering Technology is a technologically advanced program at the Bachelor of Science level utilizing theoretical concepts and hands-on instruction. Program selection is from the following concentrations: Computer Engineering Technology, Electromechanical Engineering Technology, and Mechanical Engineering Technology.. The Mechanical Engineering Technology concentration requires 67 hours is accredited by ABET, Inc. ( and enables students to obtain the skills necessary for placement in highly competitive jobs in machine design, manufacturing, engineering, field service engineering, technical sales, thermal analysis, product design, utilities operations, air conditioning design, plant operations, and a variety of other professions. Through design projects and laboratory training, students examine how to relate such skills to a variety of fields in mechanical engineering technology including product and machine design, power generation, utilities, and ...
This thesis presents a foundation for developing a business case for companies interested in the reconstructive and cosmetic procedure markets. The focus is on reviewing adipose tissue engineering research and proposing technology opportunities that could be applied to challenging soft tissue reconstruction cases and adjacently applied to cosmetic applications. To establish the foundation for this type of program, this thesis includes an evaluation of the reconstructive and cosmetic procedure markets, current practices in these markets and their constraints, as well as a literature review of research in adipose tissue engineering and its potential clinical applications. Additionally it captures the competitive landscape of major players in the reconstructive market as well as up-and-coming players in the adipose tissue engineering field. Technology development opportunities with associated customer and business value are discussed with a recommendation for the development of a detailed business ...
The cardiovascular tissue engineering laboratory aims to develop tissue engineering and cell-based therapeutic approaches for the treatment of cardiac injury and disease.
Cardiac tissue engineering is an emerging field that may hold great promise for advancing the treatment of heart diseases. Cardiac tissue engineering is in its infancy, and the overall field of tissue engineering, which was formalized in the late 1980s at conferences and workshops sponsored by the National Science Foundation, is still new enough to warrant some description. By broad definition, tissue engineering involves the construction of tissue equivalents through the manipulation and combination of living cells and biomaterials. It is a multidisciplinary field combining diverse aspects of the life sciences, engineering, and clinical medicine. The overall goal of tissue engineering is to develop tissue equivalents for use in the repair, replacement, maintenance, or augmentation of tissues or organs. Although some aspects of cardiac tissue engineering research have been ongoing for generations, albeit without being known as such, directed efforts in the field are only beginning.. The main ...
Bone marrow derived mesenchymal stem cells (bmMSCs) are widely used for the generation of tissue engineering constructs, since they can differentiate into different cell types occurring in bone tissues. Until now their use for the generation of tissue engineering constructs is limited. All cells inside a tissue engineering construct die within a short period of time after implantation of the construct because vascularization and establishment of connections to the recipient circulatory system is a time consuming process. We therefore compared the influences of bmMSC, VEGF and a combination of both on the early processes of vascularization, utilizing the mice skinfold chamber model and intravital fluorescence microscopy.. Tissue engineering constructs based on collagen coated Poly d,l-lactide-co-glycolide (PLGA) scaffolds, were either functionalized by coating with vascular endothelial growth factor (VEGF) or vitalized with bmMSC. PLGA without cells and growth factor was used as the control ...
Our MRes Tissue Engineering for Regenerative Medicine course gives students from biological, engineering and/or medical-related backgrounds the specialist knowledge and research skills to pursue a career in this field. The national average salary for a Director of Orthopedics and Tissue Engineering is $142,392 in United States. There is a $95.00 USD manuscript submission fee for all submissions to Tissue Engineering: Part A; Tissue Engineering: Part B (Reviews); and Tissue Engineering: Part C (Methods). Pittsburgh Tissue Engineering Initiative average salary is $102,300, median salary is $102,300 with a salary range from $102,300 to $102,300. The best-paid 25 percent made $114,930 that year, while the lowest-paid 25 percent made $67,830. 2 Director of Orthopedics and Tissue Engineering Salaries in Bothell provided anonymously by employees. Pittsburgh Tissue Engineering Initiative Salaries trends. ACRO Biomedical was founded in June 2014 and positioned to develop biomaterials for human tissue ...
TY - JOUR. T1 - Electrically Stimulated Adipose Stem Cells on Polypyrrole-Coated Scaffolds for Smooth Muscle Tissue Engineering. AU - Björninen, Miina. AU - Gilmore, Kerry. AU - Pelto, Jani. AU - Seppänen-Kaijansinkko, Riitta. AU - Kellomäki, Minna. AU - Miettinen, Susanna. AU - Wallace, Gordon. AU - Grijpma, Dirk. AU - Haimi, Suvi. N1 - EXT=Pelto, Jani. PY - 2016/11/14. Y1 - 2016/11/14. N2 - We investigated the use of polypyrrole (PPy)-coated polymer scaffolds and electrical stimulation (ES) to differentiate adipose stem cells (ASCs) towards smooth muscle cells (SMCs). Since tissue engineering lacks robust and reusable 3D ES devices we developed a device that can deliver ES in a reliable, repeatable, and cost-efficient way in a 3D environment. Long pulse (1 ms) or short pulse (0.25 ms) biphasic electric current at a frequency of 10 Hz was applied to ASCs to study the effects of ES on ASC viability and differentiation towards SMCs on the PPy-coated scaffolds. PPy-coated scaffolds promoted ...
Theoretical and experimental studies were performed to address the relationships between the microstructure, composition, and mechanical behaviors of articular cartilage and hydrogel-based engineered constructs for functional tissue engineering of articular cartilage. The contributions of the two major components of articular cartilage - negatively charged proteoglycans and bimodular collagen fibrils - to electromechanical properties was described by a triphasic model (Lai, Hou et al, 1991) that is incorporated with conewise linear elasticity constitutive model (Cumier, He et al 1995). The model was solved analytically for the unconfined compression stress relaxation. The fixed charge density of the tissue was successfully quantitatively calculated from stress-relaxation experiments on whole tissue samples. The interaction between collagen and proteoglycans, and the resulting residual stress and curling behaviors of cartilage strips were analyzed with a layeredinhomogeneous, orthotropic, ...
In recent years, significant success has been made in the field of regenerative medicine. Tissue engineering scaffolds have been developed to repair and replace different types of tissues. The overall goal of the current work was to develop scaffolds of native extracellular matrix components for soft tissue regeneration, more specifically, neural tissue engineering. To date, much research has been focused on developing a nerve guidance scaffold for its ability to fill and heal the gap between the damaged nerve ends. Such scaffolds are marked by several intrinsic properties including: (1) a biodegradable scaffold or conduit, consisting of native ECM components, with controlled internal microarchitecture; (2) support cells (such as Schwann cells) embedded in a soft support matrix; and (3) sustained release of bioactive factors. In the current dissertation, we have developed such scaffolds of native biomaterials including hyaluronic acid (HA) and collagen. HA is a nonsulphated, unbranched, ...
TY - JOUR. T1 - Carboxymethyl cellulose - Hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications. AU - Pasqui, Daniela. AU - Torricelli, Paola. AU - De Cagna, Milena. AU - Fini, Milena. AU - Barbucci, Rolando. PY - 2014. Y1 - 2014. N2 - Natural bone is a complex inorganic-organic nanocomposite material, in which hydroxyapatite (HA) nanocrystals and collagen fibrils are well organized into hierarchical architecture over several length scales. In this work, we reported a new hybrid material (CMC-HA) containing HA drown in a carboxymethylcellulose (CMC)-based hydrogel. The strategy for inserting HA nanocrystals within the hydrogel matrix consists of making the freeze-dried hydrogel to swell in a solution containing HA microcrystals. The composite CMC-HA hydrogel has been characterized from a physicochemical and morphological point of view by means of FTIR spectroscopy, rheological measurements, and field emission scanning electron microscopy (FESEM). No ...
Biomaterials for bone tissue engineering applications free online course video tutorial by IISc Bangalore.You can download the course for FREE !
Hydrogels are hydrophilic polymers that have a wide range of biomedical applications including bone tissue engineering. In this study we report preparation and characterization of a thermosensitive hydrogel (Zn-CS/β-GP) containing zinc (Zn), chitosan (CS) and beta-glycerophosphate (β-GP) for bone tissue engineering. The prepared hydrogel exhibited a liquid state at room temperature and turned into a gel at body temperature. The hydrogel was characterized by SEM, EDX, XRD, FT-IR and swelling studies. The hydrogel enhanced antibacterial activity and promoted osteoblast differentiation. Thus, we suggest that the Zn-CS/β-GP hydrogel could have potential impact as an injectable in situ forming scaffold for bone tissue engineering applications. Copyright © 2012 Elsevier B.V. All rights reserved.. ...
In situ tissue engineering has become a promising new technique to restore native tissue structure and function by providing a microenvironment necessary to promote tissue regeneration. A biodegradable synthetic starter matrix (scaffold) is introduced to the body to provide this microenvironment at the place of interest. By initiating an inflammatory response upon implantation, a natural wound healing process can be induced to regenerate new tissue. In time, the scaffold will be replaced by this newly formed tissue, resulting in a native, living tissue with growth potential and the capability of remodeling. Within this project, we particularly focus on using in situ tissue engineering to create living heart valves and arteries, as an alternative to the conventional heart valve and small diameter artery replacement therapies, which are accompanied by considerable decrease of life expectancy and therapy-induced complications.
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Bone is the second most transplanted tissue in the body, with approximately 2.2 million bone graft procedures performed annually worldwide. Currently, autogenous bone is the gold standard for bone grafting due to its ability to achieve functional healing; however, it is limited in supply and results in secondary injury at the donor site. Tissue engineering has emerged as a promising means for the development of new bone graft substitutes in order to overcome the limitations of the current grafts. In this research project, the specific approach for bone tissue engineering involves seeding osteoprogenitor cells within a biomaterial scaffold then culturing this construct in a biodynamic bioreactor. The bioreactor imparts osteoinductive mechanical stimuli on the cells to stimulate the synthesis of an extracellular matrix rich in osteogenic and angiogenic factors that are envisioned to guide bone healing in vivo. Fluid flow, which exerts a hydrodynamic shear stress on adherent cells, has been ...
Blood vessels mimics (BVMs) are tissue-engineered blood vessels used to test vascular devices in an environment that mimics some simple anatomical factors of native blood vessels. It is important to accurately and consistently assess tissue-engineered blood vessels, although there is currently a lack of standardization in Cal Polys Tissue Engineering Lab and in the entirety of the field. The goal of this thesis was to develop and optimize imaging and image quantification techniques for tissue-engineered blood vessels. The first aim of this thesis optimized and compared imaging and assessment techniques for electrospun scaffolds. Images from different SEMs were compared to determine the benefits and drawbacks of each microscope. Several materials were also imaged using these microscopes to characterize polymers at the microscopic scale and to compare the quality of images from different SEMs. The second aim of this thesis validated and implemented a MATLAB-based automatic fiber diameter measurement tool
Novel tissue engineering approaches are emerging to meet regenerative medicine demands and challenges towards successful therapies to completely restore the function in damaged or degenerated tissues. Among them, magnetic tissue engineering envisions the development of complex systems in which magnetic elements are exploited as remotely controlled multidimensional tools with potential for diagnostic and therapeutic actions. This chapter provides an overview of the latest developments in the design and assessment of magnetic tissue engineering strategies with particular emphasis on smart magnetic materials and their relevance for tissue regeneration. Special attention will be given to the fabrication of sophisticated systems from the nano to the macro scale, and to the role of magnetic smart materials for providing alternative approaches to address the demanding tissue requirements and meet successful alternative strategies for regenerative medicine. The cellular response to the presence of ...
Automotive Engineering Technology (BS). Electrical/Electronics Engineering Technology (BS). Manufacturing Engineering Technology (BS). Manufacturing Tooling Technology (AAS). Mechanical Engineering Technology (BS). Plastics Engineering Technology(BS). Plastics Technology (AAS). Product Design Engineering Technology (BS). Quality Engineering Technology (BS). Mechanical Engineering Technology (AAS). ...
View Notes - Stem Cells in Tissue Engineering from BIO 4400 at Cornell. Stem Cells in Tissue Engineering 4/16/07 Definition 1: g g gg p Tissue engineering is the emerging discipline of design and
Request for Customization @ Table of contents. Chapter 1. Market Synopsis. 1.1. Market Definition. 1.2. Research Scope & Premise. 1.3. Methodology. 1.4. Market Estimation Technique. Chapter 2. Executive Summary. 2.1. Summary Snapshot, 2019-2027. Chapter 3. Indicative Metrics. Chapter 4. Tissue Engineering Market Segmentation & Impact Analysis. 4.1. Tissue Engineering Market Material Segmentation Analysis. 4.2. Industrial Outlook. 4.2.1. Market indicators analysis. 4.2.2. Market drivers analysis. Rising chronic condition incidences. Rising prevalence of disorders associated with the kidney. Increasing resistance to animal use in medical research. Growing demand for tissue engineering processes and regenerative medicine. 4.2.3. Market restraints analysis. The high cost of treatment with tissue engineering. Lack of tissue engineering awareness. 4.3. Technological Insights. 4.4. ...
TY - JOUR. T1 - Principles of cell mechanics for cartilage tissue engineering. AU - Shieh, Adrian C.. AU - Athanasiou, Kyriacos A.. PY - 2003. Y1 - 2003. N2 - The critical importance of mechanical signals to the health and maintenance of articular cartilage has been well demonstrated. Tissue engineers have taken a cue from normal cartilage physiology and incorporated the use of mechanical stimulation into their attempts to engineer functional cartilage. However, the specific types of mechanical stimulation that are most beneficial, and the mechanisms that allow a chondrocyte to perceive and respond to those forces, have yet to be elucidated. To develop a better understanding of these processes, it is necessary to examine the mechanical behavior of the single chondrocyte. This paper reviews salient topics related to chondrocyte biomechanics and mechanotransduction, and attempts to put this information into a context both appropriate and useful to cartilage tissue engineering. It also describes ...
To date, most in vitro and in vivo studies in the field of cardiovascular tissue research rely on the conventional monolayer (2D) cell cultures. Such 2D culture systems may introduce false positive and/or negative results in the mechanistic studies and translational applications primarily due to the microenvironment of 2D cultures that substantially differ from the in vivo cardiovascular cellular and extracellular matrix (ECM) organizations. Recently, it is found that transition from conventional monolayer cell cultures to 3D culture systems contributes to a closer recapitulation of in vivo features, such as cell heterogeneity, ECM, cell signalling, proliferation, maturation, and response to stimuli. Moreover, recent advances in 3D histotypic and organotypic cultures have escalated the impact and scope in the studies of cardiovascular development, diseases, and therapies. For example, the engineered heart tissue/muscle and cardiovascular spheroids have shown great promises in the in vitro modelling of
Myocardial Infarction leads to end-stage heart failure and it is the major cause of death in many industrialized nations. Tissue engineering approaches for treatment of the infarcted tissue has gained huge attention over the recent years and research in this direction mainly aims for the optimization of a biomaterial scaffold with cell-source for tissue regeneration. In this regard, we fabricated absolutely natural polymeric composite scaffolds, using the blood protein, namely fibrinogen, the denatured collagen glycoprotein gelatin and collagen by electrospinning process. Scaffolds with different weight ratios of fibrinogen:gelatin (Fib:Gel) and Fibrinogen:Collagen (Fib:Coll) was prepared and cross-linking (CL) of the electrospun scaffolds was carried out using glutaraldehyde vapors to improve their mechanical properties. The fiber diameters of the fabricated scaffolds were in the range of 150 ? 300 nm which was close to the size of the native protein fibers in the myocardial extracellular ...
Advances in Materials Science and Engineering is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles in all areas of materials science and engineering.
Cartilage tissue engineering remains a top priority due to the limited intrinsic capacity of articular cartilage for self-repair. In this study, the tissue engineering potential of a decellularized porcine cartilage scaffold, in which the proteoglycans (PG) had also been removed, was evaluated. To improve cell distribution within the scaffold, a novel cell seeding technique using centrifugation and a cell seeding device designed for this technique was developed. The modified porcine cartilage scaffolds were seeded with chondrocytes using the novel cell seeding technique and left in static culture for up to 21 days. A previously described bioreactor was used to measure the properties of the constructs at 7, 14, and 21 days. The ability of the scaffolds to support cell viability and proliferation and extracellular matrix deposition was evaluated at these time points as well. The novel cell seeding technique was also evaluated at 24 hours. Results indicated that the scaffold was capable of supporting cell
The development of human liver scaffolds retaining their 3-dimensional structure and extra-cellular matrix (ECM) composition is essential for the advancement of liver tissue engineering. We report the design and validation of a new methodology for the rapid and accurate production of human acellular …
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Tissue Engineering Society International Annual Conference, Oct. 24, 2005. First Clinical Use of Tissue Engineered Blood Vessels in the Adult Arterial Circulation. Nicolas L´Heureux, Todd McAllister, Sergio Garrido, Alicia Marini, Hernan Avila, Luis de la Fuente, Ximena Manglano, Robert C Robbins, Gerhardt Konig, Nathalie Dusserre. The historical challenge in the field of Cardiovascular Tissue Engineering has been to produce a conduit with the appropriate mechanical strength (i.e. burst pressure in excess of 1700 mmHg). To achieve the requisite strength, most approaches have relied upon permanent synthetic or xenogeneic scaffolds. These scaffolds, however, may be associated with chronic inflammation, disease transmission, or mechanical degradation that limit their clinical use. In an effort to eliminate the deleterious effects of permanent biomaterials, more recent trends have focused on either resorbable scaffolds or completely biological approaches. Previously we reported a completely ...
We have previously reported on the use of Bay K8644-release strategies in combination with perfusion-compression bioreactor systems for up regulating bone formation in three-dimensional PLLA scaffolds. Here we report on the analysis of Bay activity following its release from our PLLA scaffolds over the culture period imposed in our tissue engineering protocol using UV spectroscopy in combination with whole cell patch clamping techniques. Bay was released continually from scaffolds within the physiological range required for agonist activity (1-10 microM). Patch clamping allowed for the effects of Bay released from scaffolds to be monitored directly with respect to osteoblast electrophysiology. A characteristic shift in the current-voltage (I-V) relationship of L-type VOCC currents was observed in rat osteoblast sarcoma (ROS) cells patched in a solution with Bay released from scaffolds following 14 and 28 days incubation, with statistically significant differences observed in peak currents compared to
Autologous tissue-engineered blood vessels (TEBVs) generated using adult stem cells have shown promising results, but many preclinical evaluations do not test the efficacy of stem cells from patient populations likely to need therapy (i.e., elderly and diabetic humans). Two critical functions of these cells will be (i) secreting factors that induce the migration of host cells into the graft and (ii) differentiating into functional vascular cells themselves. The purpose of this study was to analyze whether adipose-derived mesenchymal stem cells (AD-MSCs) sourced from diabetic and elderly patients have a reduced ability to promote human smooth muscle cell (SMC) migration and differentiation potential toward SMCs, two important processes in stem cell-based tissue engineering of vascular grafts ...
ARTEC - Advanced Regenerative Tissue Engineering Centre. Looking for abbreviations of ARTEC? It is Advanced Regenerative Tissue Engineering Centre. Advanced Regenerative Tissue Engineering Centre listed as ARTEC
Cardiac tissue engineering (CTE) is currently a prime focus of research due to an enormous clinical need. In this work, a novel functional material, Poly(3-hydroxyoctanoate), P(3HO), a medium chain length polyhydroxyalkanoate (PHA), produced using bacterial fermentation, was studied as a new potential material for CTE. Engineered constructs with improved mechanical properties, crucial for supporting the organ during new tissue regeneration, and enhanced surface topography, to allow efficient cell adhesion and proliferation, were fabricated. Our results showed that the mechanical properties of the final patches were close to that of cardiac muscle. Biocompatibility of the P(3HO) neat patches, assessed using Neonatal ventricular rat myocytes (NVRM), showed that the polymer was as good as collagen in terms of cell viability, proliferation and adhesion. Enhanced cell adhesion and proliferation properties were observed when porous and fibrous structures were incorporated to the patches. Also, no ...
CR (n = 10) were removed from the bypass system after surgery. Isolation was performed using different isolation methods: blood samples were taken from the cardiopulmonary bypass and centrifuged at low density. The venous filter screen was cut out and placed into petri dishes for cultivation. The spongelike filter was removed, washed and treated in the same way as the blood samples. After cultivation, cell lines of fibroblasts (FB) and endothelial cells (EC) were obtained for analysis. The cells were seeded on polyurethane patches and analyzed via scanning electron microscopy (SEM), Life/Dead assay and immunohistochemistry.. ...
THESIS 8757 Tissue engineering (or regenerative medicine) is defined as the application of scientific principles to the synthesis of living tissues using bioreactors, cells, scaffolds, growth factors, or a combination (Rose and Oreffo, 2002). One of the principal methods in tissue engineering involves the use of a porous scaffold to support and guide synthesis of a 3D tissue or organ (Sachlos and Czernuszka, 2003). Collagen-Glycosaminoglycan scaffolds have found success in several clinical applications of tissue engineering (Yannas et al., 1989, Chamberlain et al., 1998). ...
Bone and Cartilage Engineering provides a complete overview of recent knowledge in bone and cartilage tissue engineering. It follows a logical approach to the various aspects of extracorporal bone and cartilage tissue engineering. The cooperation between a basic scientist and a clinician made it possible to structure the books content and style according to the interdisciplinary character of the field. The comprehensive nature of the book, including detailed descriptions of laboratory procedures, preclinical approaches, clinical applications, and regulatory issues, will make it an invaluable basis for everyone working in this field. This book will serve as a fundamental tool for basic researchers to establish or refine tissue engineering techniques as well as for clinicians to understand and use this modern therapeutic option. ...
This review discusses the role of the cannabinoid system in cartilage tissue and endeavors to establish if targeting the cannabinoid system has potential in mesenchymal stem cell based tissue-engineered cartilage repair strategies. The review discusses the potential of cannabinoids to protect against the degradation of cartilage in inflamed arthritic joints and the influence of cannabinoids on the chondrocyte precursors, mesenchymal stem cells (MSCs). We provide experimental evidence to show that activation of the cannabinoid system enhances the survival, migration and chondrogenic differentiation of MSCs, which are three major tenets behind the success of a cell-based tissue-engineered cartilage repair strategy. These findings highlight the potential for cannabinoids to provide a dual function by acting as anti-inflammatory agents as well as regulators of MSC biology in order to enhance tissue engineering strategies aimed at cartilage repair.
Until now, Tissue Engineering techniques have frequently been shown to be promising in vitro and in vivo in experimental settings, but have widely failed to enter the clinical routine when it comes to large defects or major organ functional replacements [13]. One of the key roles for this insufficient transition into clinical practice has been discussed to be dependent on the lack of sufficient vasculature at the time of transplanting laboratory-grown constructs into relevant and especially into poorly vascularized recipient areas. Large bone defects present a prototype of such difficult to handle clinical replacement problems, as vascularized bone grafts are associated with a significant donor-site morbidity and non-vascularized bone grafts do not heal into problematic sites. The optimal bone graft for any successful reconstruction of a large osseus defect would consist of a custom-designed vascularized bone substitute without creating any donor-site morbidity. Almost all bone tissue ...
The development of a functional tissue-engineered human skeletal muscle model in vitro would provide an excellent platform on which to study the process of myogenesis, various musculoskeletal disease states, and drugs and therapies for muscle toxicity. We developed a protocol to culture human skeletal muscle bundles in a fibrin hydrogel under static conditions capable of exerting active contractions. Additionally, we demonstrated the use of joint miR-133a and miR-696 inhibition for acceleration of muscle differentiation, elevation of active contractile force amplitudes, and increasing Type II myofiber formation in vitro. The global hypothesis that motivated this research was that joint inhibition of miR-133a and miR-696 in isolated primary human skeletal myoblasts would lead to accelerated differentiation of tissue-engineered muscle constructs with higher proportion of Type I myofibers and that are capable of significantly increased active contractile forces when subjected to electrical ...
Bektas, C. K., & Hasirci, V. (2018). Mimicking Corneal Stroma Using Keratocyte Loaded Photopolymerizable Methacrylated Gelatin Hydrogels. Journal of Tissue Engineering and Regenerative Medicine.. ...
Sigma-Aldrich offers abstracts and full-text articles by [Paul W Riem Vis, Carlijn V C Bouten, Joost P G Sluijter, Gerard Pasterkamp, Lex A van Herwerden, Jolanda Kluin].
The US Food and Drug Administration approves a device, seeded with a patient’s own cells, which can help repair damaged knee cartilage—a first for autologous cartilage technology.
Worldwide market for Tissue Engineering technologies explored in this study includes Cell Culture, Immunomodulation and Stem Cell. The report also focuses on therapeutic applications of tissue engineering comprising Cardiovascular, Dental/Oral Neurological, Oncology, Orthopedic, Skin/Integumentary and Others. The markets for the above mentioned technologies and therapeutic applications are analyzed in terms of USD. Global market for Tissue Engineering, estimated at US$23 billion in 2015, forecast to reach US$27.3 billion in 2015, and is further expected to register a CAGR of about 23% between 2016 and 2022 to touch a projected US$94.2 billion by 2022.
Nanotechnology-enabled tissue engineering is receiving increasing attention. The ultimate goal of tissue engineering as a medical treatment concept is to replace or restore the anatomic structure and function of damaged, injured, or missing tissue. At the core of tissue engineering is the construction of three-dimensional scaffolds out of biomaterials to provide mechanical support and guide cell growth into new tissues or organs. Biomaterials can be variously permanent or biodegradable, naturally occurring or synthetic, but inevitably need to be biocompatible. Using nanotechnology, biomaterial scaffolds can be manipulated at atomic, molecular, and macromolecular levels. Creating tissue engineering scaffolds in nanoscale also may bring unpredictable new properties to the material, such as mechanical (stronger), physical (lighter and more porous) or chemical reactivity (more active or less corrosive), which are unavailable at micro- or macroscales. For bone tissue engineering, a special subset of ...
Cardiac tissue regeneration is an integrated process involving both cells and supporting matrix. Cardiomyocytes and stem cells are utilized to regenerate cardiac tissue. Hydrogels, because of their tissue-like properties, have been used as supporting matrices to deliver cells into infarcted cardiac muscle. Bioactive and biocompatible hydrogels mimicking biochemical and biomechanical microenvironments in native tissue are needed for successful cardiac tissue regeneration. These hydrogels not only retain cells in the infarcted area, but also provide support for restoring myocardial wall stress and cell survival and functioning. Many hydrogels, including natural polymer hydrogels, synthetic polymer hydrogels, and natural/synthetic hybrid hydrogels are employed for cardiac tissue engineering. In this review, types of hydrogels used for cardiac tissue engineering are briefly introduced. Their advantages and disadvantages are discussed. Furthermore, strategies for cardiac regeneration using hydrogels are
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Macroporous hydroxyapatite scaffolds for bone tissue engineering applications: Physicochemical characterization and assessment of rat bone marrow stromal cells ...
This project will develop and demonstrate a prototype Modular Perfusion Bioreactor (MPB) for tissue engineering applications. While many unique styles of bioreactors have been proposed for various types of stem cell and tissue cultures, there is not a single, easy-to-use device that accommodates the multiple diverse needs of multiple tissue culture types.. In order to take advantage of recent advances in stem cell culture, biomaterials, and tissue engineering techniques, Resodyn Corporation proposes to develop, design, fabricate, and test a multi-functional bioreactor platform system. As a starting point for this multi-functional system, Resodyn Corporation will use its highly scalable (50-1,500ml) and successful (>1x108 cells/ml) hypoxia perfusion bioreactor. The focus of the proposed work will be to design additional culture modules that can be plugged into an advanced platform system developed by Resodyn Corporation. Modules that allow the culture of cellular monolayers, encapsulated cells, ...
Kortsmit J, Driessen NJ, Rutten MC, Baaijens FP. Nondestructive and noninvasive assessment of mechanical properties in heart valve tissue engineering. Tissue Eng Part A. 2009 Apr; 15(4):797-806 ...
TY - JOUR. T1 - Effective decellularisation of human saphenous veins for biocompatible arterial tissue engineering applications. T2 - bench optimisation and feasibility in vivo testing. AU - Sulaiman, Nadiah B. AU - Bond, Andrew R. AU - Bruno, Vito Domenico. AU - Joseph, John. AU - Baz Lopez, Daniel. AU - Johnson, Jason L. AU - Suleiman, M-Saadeh. AU - George, Sarah J. AU - Ascione, Raimondo. PY - 2020/12/22. Y1 - 2020/12/22. KW - Decellularisation. KW - Bioengineering. KW - Tissue Engineering. KW - Vascular Graft. M3 - Article (Academic Journal). JO - Journal of Tissue Engineering. JF - Journal of Tissue Engineering. SN - 2041-7314. ER - ...
Mol, A; Hoerstrup, S P (2004). Heart valve tissue engineering -- where do we stand? International Journal of Cardiology, 95(Suppl 1):S57-S58. ...
Scaffold-based tissue engineering requires for transplanted or host cells a biodegradable matrix, which provides a specific environment for tissue development. Efficiency of tissue regeneration through cell implantation in scaffolds depends mainly on the architecture of the scaffold and on the properties of the biomaterial used for their fabrication. The scaffold architecture is characterized by the pore shape and size, size distribution, pore interconnectivity and throat size. Among the polymers selected for tissue engineering, polyurethanes (PUR) represent a very important group. By varying the molecular weight of polyol and the composition of the hard segments, properties of PUR can be tuned for use in tissue engineering, either for reconstruction of soft tissue or for cartilage and bone regeneration. The objective of this study was to characterize polyurethane porous scaffolds fabricated by the salt-leaching/polymer coagulation method. The effect of solution concentration and salt particles ...
In the industrialized world, cardiovascular disease alone is responsible for almost half of all deaths. Many of the conditions can be treated successfully with surgery, often using transplantation techniques; however, autologous vessels or human-donated organs are in short supply. Tissue engineering aims to create specific, matching grafts by growing cells on appropriate matrices, but there are many steps between the research laboratory and the operating theatre. Neo-tissues must be effective, durable, non-thrombogenic and non-immunogenic. Scaffolds should be bio-compatible, porous (to allow cell/cell communication) and amenable to surgery. In the early days of cardiovascular tissue engineering, autologous or allogenic cells were grown on inert matrices, but patency and thrombogenicity of grafts were disappointing. The current ethos is toward appropriate cell types grown in (most often) a polymeric matrix that degrades at a rate compatible with the cells production of their own extracellular ...
TY - JOUR. T1 - Investigating breast cancer cell behavior using tissue engineering scaffolds. AU - Guiro, Khadidiatou. AU - Patel, Shyam A.. AU - Greco, Steven J.. AU - Rameshwar, Pranela. AU - Arinzeh, Treena L.. N1 - Publisher Copyright: © 2015 Guiro et al. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 2015/4/2. Y1 - 2015/4/2. N2 - Despite early detection through the use of mammograms and aggressive intervention, breast cancer (BC) remains a clinical dilemma. BC can resurge after ,10 years of remission. Studies indicate that BC cells (BCCs) with self-renewal and chemoresistance could be involved in dormancy. The majority of studies use in vitro, two-dimensional (2-D) monolayer cultures, which do not recapitulate the in vivo microenvironment. Thus, to determine the effect of three-dimensional (3-D) microenvironment on BCCs, this study fabricated tissue engineering scaffolds made of poly (ε-caprolactone) (PCL) having aligned or random fibers. Random and aligned fibers ...
Electrospun tissue engineering scaffolds are attractive due to their distinctive advantages over other types of scaffolds. As both osteoinductivity and osteoconductivity play crucial roles in bone tissue engineering, scaffolds possessing both properties are desirable. In this investigation, novel bicomponent scaffolds were constructed via dual-source dual-power electrospinning (DSDPES). One scaffold component was emulsion electrospun poly(D ,L -lactic acid) (PDLLA) nanofibers containing recombinant human bone morphogenetic protein (rhBMP-2), and the other scaffold component was electrospun calcium phosphate (Ca-P) particle/poly(lactic-co-glycolic acid) (PLGA) nanocomposite fibers. The mass ratio of rhBMP-2/PDLLA fibers to Ca-P/PLGA fibers in bicomponent scaffolds could be controlled in the DSDPES process by adjusting the number of syringes used to supply solutions for electrospinning. Through process optimization, both types of fibers could be evenly distributed in bicomponent scaffolds. The ...
Patients with critical-size bone defects, as a result of trauma, congenital malformations or tumor resections, generally have limited healing without clinical intervention. The autograft is the current standard of care for repair of these defects due to capacity for osteointegration and immunological compatibility. However, potential limitations, such as donor site morbidity, have motivated the development of alternative autologous approaches for the treatment of these defects. Materials used in tissue engineering, such as scaffolds, growth factors and adult stem cells, can be derived from patient blood and adipose tissue and are potential autologous therapeutic options. This dissertation investigates a prospective procedure to improve craniofacial bone healing using fibrin scaffolds and platelet rich plasma from patient blood, and adipose-derived stem cells from liposuction. The objectives of these studies are to evaluate the effects of fibrin scaffolds and platelet-rich plasma on ...
Biological compatibility of a biological derivation bone tissue engineering scaffold was all sidedly evaluated by biological test of basic and additional evaluation. Results showed that the grades of cell culture with the material were grade Ⅰ. There was no sensitization effect; no irritant reactions were found in test of genotoxicity and test of chronic toxicity, there was no irritant reaction to the material implanted in bone and the hemolytic rate was 0 61%. The results demonstrated that the biological derivation bone tissue engineering scaffold is a satisfactory biomaterial.
Learn how Dr. Sam Pashneh-Tala from the University of Sheffield uses SLA 3D printing to enable the production of tissue-engineered blood vessels.
The histomorphometrical analysis of the total scans permitted an evaluation and comparison of the respective cell distribution in the PRF clots. The total length of each clot was measured and a mean of ± SEM was calculated. The distribution/allocation of each cell type was evaluated in the corresponding total scan of the immunohistochemical staining. The analyses revealed that platelets were the only ones found in each area of the clot up to 87 ± 13% in the S-PRF group and up to 84 ± 16% in the A-PRF group (Figure 5). Furthermore, the results showed that T-lymphocytes (S-PRF: 12 ± 5%, A-PRF: 17 ± 9%), B-lymphocytes (S-PRF: 14 ± 7%, A-PRF: 12 ± 9%), CD34-positive stem cells (S-PRF: 17 ± 6%, A-PRF: 21 ± 11%), and monocytes (S-PRF: 19 ± 9%, A-PRF: 22 ± 8%) were not found beyond a certain point of maximally 30% of the total clot length, as they are distributed in or near the BC generated by the centrifugation process (Figure 5). Statistical analysis revealed no statistically significant ...
The tissue engineering and biomaterials research thrust in the Department of Biomedical Engineering focuses on the development of new materials for applications in medicine and biology as well as on engineering biological tissues from adult stem cells. Specific areas of active research include cardiovascular tissue engineering, biopolymers, nitric oxide releasing materials for improved biocompatibility, tissue-biomaterial interaction, and biomimetic materials.
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The field of tissue engineering has advanced and evolved to focus on biomimetic strategies to meet the rise in demands of tissue replacements for surgical reconstruction. One of the key strategies focuses on developing growth factor delivery systems, by incorporating growth factors into tissue scaffolds. While growth factors are crucial cell-inducing components, their limitations such as short half-lives and dose related adverse effects remain a challenge. To overcome these challenges, this thesis is focused on the development of a novel biomimetic tissue scaffold concept incorporating cell-mediated activation of growth factors for cartilage regeneration. The latent transforming growth factor-β1 (TGF-β1) was selected as a model latent protein due to its well established effects on cartilage as well as its ubiquity in many other tissue types. The thesis first focused on the development and characterisation of the tissue scaffold. A non-woven fibrous scaffold was fabricated by electrospinning, ...
Ver más] Tissue engineering is an emerging field of research which combines the use of cell-seeded biomaterials both in vitro and/or in vivo with the aim of promoting new tissue formation or regeneration. In this context, how cells colonize and interact with the biomaterial is critical in order to get a functional tissue engineering product. Cell-biomaterial interaction is referred to here as the phenomenon involved in adherent cells attachment to the biomaterial surface, and their related cell functions such as growth, differentiation, migration or apoptosis. This process is inherently complex in nature involving many physico-chemical events which take place at different scales ranging from molecular to cell body (organelle) levels. Moreover, it has been demonstrated that the mechanical environment at the cell-biomaterial location may play an important role in the subsequent cell function, which remains to be elucidated. In this paper, the state-of-the-art research in the physics and mechanics ...
Polymeric multilayered capsules (PMCs) have found great applicability in bioencapsulation, an evolving branch of tissue engineering and regenerative medicine. Here, we describe the production of hierarchical PMCs composed by an external multilayered membrane by layer-by-layer assembly of poly(L-lysine), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface functionalized collagen II-TGF-β3 poly(L-lactic acid) microparticles for cartilage tissue engineering.. ...
Acellular matrix obtained from homologous muscular tissue has been previously used to repair muscular defects. However, the implants, although not rejected, give rise to an intense inflammatory response and are rapidly replaced by fibrous tissue. In this study we examined the possibility that co-culture with autologous satellite cells can improve the efficiency of homologous acellular matrix as skeletal muscle substitute. Satellite cells, isolated from rat dorsal muscle, were cultured in vitro on homologous acellular matrix obtained by detergent-enzymatic treatment of abdominal muscle fragments. Scanning electron microscopy revealed that after 24 h of co-culture satellite cells were attached to the matrix, but still possessed a round shape. After 96 h, seeded cells began to flatten and to differentiate, originating few multinucleated myotubes. Patches of homologous matrix, seeded or not with autologous satellite cells, were implanted in the dorsal muscle of rats. At autopsy, the implants were ...
Injuries to articular cartilage are one of the most challenging issues of musculoskeletal medicine due to the poor intrinsic ability of this tissue for repair. Despite progress in orthopaedic surgery, the lack of efficient modalities of treatment for large chondral defects has prompted research on tissue engineering combining chondrogenic cells, scaffold materials and environmental factors. The aim of this review is to focus on the recent advances made in exploiting the potentials of cell therapy for cartilage engineering. These include: 1) defining the best cell candidates between chondrocytes or multipotent progenitor cells, such as multipotent mesenchymal stromal cells (MSC), in terms of readily available sources for isolation, expansion and repair potential; 2) engineering biocompatible and biodegradable natural or artificial matrix scaffolds as cell carriers, chondrogenic factors releasing factories and supports for defect filling, 3) identifying more specific growth factors and the appropriate
The objective of this article is to systematically present the emerging understanding that 3D porous scaffolds serve not only as structural templates for tissue fabrication but also provide complex signaling cues to cells and facilitate oxygen and therapeutic agent delivery. Strategies in the field of tissue engineering and regenerative medicine often rely on 3D scaffolds to mimic the natural extracellular matrix as structural templates that support cell adhesion, migration, differentiation and proliferation, and provide guidance for neo-tissue formation. In addition to providing a temporary support for tissue fabrication, 3D scaffolds have also been used to study cell signaling that best mimics physiological conditions, thereby expanding our understanding beyond 2D cell cultures. It is now understood that cell responses to 3D scaffolds are distinctively different from 2D surfaces. Recently, 3D scaffolds emerged as a vehicle for improved oxygen transport to seeded cells and also to deliver relevant
Many strategies for tissue engineering of replacement structures, such as heart valves, depend in part on preparing a tissue scaffold from a natural tissue matrix. An essential step in tissue scaffold fabrication is decellularization of the matrix. The objective of any decellularization method is twofold: (1) the preservation of the physical and biochemical properties of the extracellular matrix (ECM), and (2) the removal of all cellular material. Decellularization is currently done by contacting xenographic tissue with a combination of chemical detergents and biological agents. These processes can alter ECM structure and composition, which can trigger host immune response and inflammation. Currently, there are no accepted quantitative standards against which to certify the viability of the decellularized material. However, future standards will certainly include characteristics like removal of nuclear material, biochemical composition, and mechanical strength. We are evaluating a novel ... provides the latest news on engineering technology, engineering science, computer engineering , civil engineering, chemical engineering, aerospace engineering and environmental engineering.( ... sorted: liverank/all)(... continued page 6)
Despite huge efforts, tissue engineers and orthopedic surgeons still face a great challenge to functionally repair osteochondral (OC) defects. Nevertheless, over the past decade great progress has been made to find a suitable strategy towards OC regeneration. In the clinics, some osteochondral tissue engineering (OCTE) approaches have already been applied although with some incongruous outcomes as OC tissue is complex in its architecture and function. In this chapter, we summarize current OCTE strategies that are focused on hierarchical scaffold design, mainly layered scaffolds. Most suitable candidates towards functional regeneration of OC tissues are envisaged from monophasic to the layered scaffolds. Herein is documented a variety of approaches with their intrinsic properties applied as bare scaffolds or in combination with biologics, either in in vitro or in vivo evaluations aiming at functional OC regeneration. The most noteworthy studies in OC regeneration developed within the past 5 years ...
TY - JOUR. T1 - Application of stem cells for articular cartilage regeneration.. AU - Hwang, Nathaniel S.. AU - Elisseeff, Jennifer. PY - 2009/1. Y1 - 2009/1. N2 - Articular cartilage is a highly organized tissue lacking self-regeneration capacity upon lesion. Current surgical intervention by application of in vitro-expanded autologous chondrocytes transplantation procedure is associated with several disadvantages, including donor-site morbidity and inferior fibrocartilage formation at the defect site. However, recent advancements in tissue engineering have provided notable strategies for stem cell-based therapies and articular cartilage tissue engineering. In this review, we discuss the current strategies to engineer cartilage tissues from adult stem cells and human embryonic stem cell-derived cells. The characteristics of adult stem cells, the microenvironmental control of cell fate determination, and the limitation imposed by the intrinsic nature of stem cells are discussed. The strategy to ...
Worldwide, an estimated 2.5 million people live with spinal cord injury, with more than 130,000 new injuries reported each year. Spinal cord injury has a significant impact on patients quality of life, life expectancy and economic burden, with considerable costs associated with primary care and loss of income. Stroke is currently the second leading cause of death in the Western world, ranking after heart diseases and before cancer, and could raise secondary dysfunctions too. In the case of focal brain ischemia and chronic spinal cord injuries, namely whenever an extensive loss of tissue occurs, cell therapy is helpful but not sufficient for the regeneration of the lost tissues. Within these regions, scaffolds are needed in order to provide physical support for axonal regeneration and for the transplanted cells to effectively integrate within the host tissues. To this purpose, tissue engineering, an interdisciplinary field of medical science bringing together the principles of material science, ...
0001]An enormous expenditure of health-care resources was required for the repair and replacement of diseased tissue structures and organs. The most common treatment, replacement with an autograft, produces less than optimal results. However, the supply of autograft, and even allograft, is very limited. Engineering tissues and organs with mammalian cells and a scaffolding material as emerged as a promising alternative approach in the treatment of malfunctioning or lost organs compared to the use of harvested tissues and organs (see Langer, R. S. and J. P. Vacanti, Tissue engineering: the challenges ahead, Scientific American 280(4), 86 (1999)). In this approach, a temporary scaffold is needed to serve as an adhesive substrate for the implanted cells and a physical support to guide the formation of the new organs. Accordingly, the scaffold materials must be custom-engineered to match the biomechanical, biochemical, and biological needs of the specific tissue or organ they are designed to ...
Cardiac patch, which is a suitable alternative to heart transplant, recovers the heart tissue and ensures its sound functioning. The integration of advanced features such as therapeutic control through drug release is another groundbreaking innovation which is expected to be introduced in the cardiac patches and marketed globally in the coming decade. Such improvisations and additional features to the existing cardiac patches is anticipated to fuel the growth of the global cardiac patch market significantly during the 2017-2025 period. However, the limitations of cardiac patches such as the formation of aneurysms and the obstruction of growth potential because of ingrown tissue and calcification leads to multiple replacements and can hamper the market growth.. The global cardiac patch market can be segmented based on end-users and region. In terms of end-users, the market can be divided into hospitals and specialty clinics. The hospitals segment is likely to hold a major share in the global ...
TY - JOUR. T1 - Characterization of chitosan-gelatin scaffolds for dermal tissue engineering. AU - Tseng, Hsiang Jung. AU - Tsou, Tai Li. AU - Wang, Hsian Jenn. AU - Hsu, Shan hui. PY - 2013/1. Y1 - 2013/1. N2 - Porous scaffolds for dermal tissue engineering were fabricated by freeze-drying a mixture of chitosan and gelatin (CG) solutions. Different crosslinking agents including glutaraldehyde, 1-(3-dimethylaminopropyl)-3-ethyl-carbodimide hydrochloride (EDC), and genipin were used to crosslink the scaffolds and improve their biostability. The porous structure and mechanical properties were determined for the scaffolds. The proliferation of human fibroblasts in the scaffolds was analyzed. It was found that EDC crosslinked scaffolds had the greatest amount of cells after four days. EDC crosslinked CG scaffolds had tensile modulus in a dry state and compressive modulus in a wet state similar to commercial collagen wound dressing. They also showed appropriate pore size, high water absorption, and ...
McFetridge, P. S., Abe, K., Horrocks, M. and Chaudhuri, J. B., 2007. Vascular tissue engineering: Bioreactor design considerations for extended culture of primary human vascular smooth muscle cells. Asaio Journal, 53 Sep-Oct (5), pp. 623-630.. ...
Purpose : Upon injury to the cornea, the composition of the extracellular matrix (ECM) rapidly changes to promote wound healing through its interactions with integrins. We hypothesize that ECM remodelling occurring during corneal wound healing causes the activation of very specific signal transduction mediators that favor faster closure of the wound. Our goal is to proceed to the pharmacological inhibition and/or activation of the PI3K/Akt mediators Akt and CREB using the human tissue-engineered cornea (hTECs) as a model. Methods : hTECs produced by the self-assembly approach were wounded with a 8-mm diameter biopsy punch and deposited on another reconstructed human corneal stroma to allow wound closure on a natural ECM. Total RNAs and proteins were prepared from the epithelial cells of wounded and unwounded areas and their gene expression pattern was determined by microarrays. The wounded tissues were then incubated with or without C646 (a CREB inhibitor) or with or without SC79 (an AKT ...
Learn more about stem cells by reading Stem Cell-Based Bone Tissue Engineering with a Hydrogel Scaffold Shows Promise for Bone Repair on the Stemodontics® website.
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Thomas Gaborskis research may be in ultra-thin nano-membranes, but its going to be titanic in advancing tissue engineering.. Gaborski, assistant professor of biomedical engineering at Rochester Institute of Technology, and his research team are developing ways to use ultra-thin nano-membranes and adipose stem cells to create the vascular network necessary in engineering tissue, skin and organs.. For these organs to be viable, there is a need for not only the organ structure but also the inner network of micro-vessels and capillaries. Gaborski is helping develop that complex structure, using transparent and permeable membrane scaffolds to support cell and tissue growth, essential to tissue engineering.. Using adipose-derived stem cells that come from fat tissue, acquired from adults rather than embryos, Gaborski has been able to create functional microenvironments that help support and differentiate stem cells into the specialized cells that make up the human body. Creating engineered tissues ...
This review detailed the most commonly used biomaterial scaffolds for engineering tissues from stem cells by covering the types of materials available and their unique properties. This information allows readers to determine which material best suits their specific application. As mentioned in earlier in this review, many of these materials have not been fully optimized for specific tissue engineering applications and further work will continue to optimize these formulations for translation to the clinic for targeted applications. For example, optimized scaffolds could enhance the survival and differentiation of neural stem cells being transplanted into the diseased or damaged nervous system, which could lead to improved function. The type of material and the cues that are incorporated in the scaffold play a large role in directing the fate of the stem cells seeded inside as detailed in this review. The ability to further functionalize the materials discussed in this review in terms of their ...
QMULs School of Engineering and Materials Science (SEMS) provides outstanding degree programmes coupled with internationally leading research: Study reveals how manipulation of primary cilia may improve cartilage tissue engineering
2002). The tortuosity of scaffolds fabricated using solvent casting is not controllable because it requires the contact of the particulates during the fabrication procedure, which is a random process facilitated by using high humidity. Similarly, poor interconnectivity of pores is reported for scaffolds produced using gas foaming techniques, where only 10%À30% of the scaffolds pores are connected (Hutmacher, 2001). Control of pore size distribution in scaffolds fabricated using freeze-drying is an interesting research topic. 2010. The acellular matrix (ACM) for bladder tissue engineering: a quantitative magnetic resonance imaging study. Magn. Reson. Med. 64 (2), 341À348. 22404. , 2014. One-pot synthesis of macromesoporous bioactive glasses/polylactic acid for bone tissue engineering. Mater. Sci. Eng. C Mater. Biol. Appl. 43, 367À374. 1016/j. 042. Epub 2014 Jul 19. , 2014. Designer functionalised selfassembling peptide nanofibre scaffolds for cartilage tissue engineering. Expert Rev. Mol. ...
TY - JOUR. T1 - Combining back scattered electron microscopy and secondary emission scanning electron microscopy to study articular cartilage morphology without decalcifying and staining the samples. AU - Merolli, A.. AU - Manunta, A.. AU - Phillips, Gary. AU - Santin, Matteo. AU - Catalano, F.. PY - 2010/8/13. Y1 - 2010/8/13. U2 - 10.3969/j.issn.1673-8225.2010.33.001. DO - 10.3969/j.issn.1673-8225.2010.33.001. M3 - Article. VL - 14. SP - 6081. EP - 6086. JO - Journal of Clinical Rehabilitative Tissue Engineering Research. JF - Journal of Clinical Rehabilitative Tissue Engineering Research. SN - 1673-8225. IS - 33. ER - ...
Stem cells have shown huge potential for regenerative medicine, but there are several critical issues to be addressed to further improve therapeutic efficacy and regenerative potential of stem cell therapy and stem cell-based tissue engineering. Functional biomaterials can solve these the limitations of current stem cell therapy by promoting prolieration, specific lineage differentiation and improving in vivo survival and engraftment of transplanted stem cells. This thematic series in Biomaterials Research aims to provide collections of recent studies on developing biomaterials for improving stem cell-based regenerative medicine.. To submit a paper to this series, please visit the Biomaterials Research submission site. Make sure to choose the name of the series under the Are you submitting to a thematic series? question on the Additional information tab.. New articles in the series will appear here as they are published.. ...
Dr. Lee has spearheaded in the development of in vitro tissue models and novel bioreactors in the field of cardiovascular tissue engineering in the past several years. She has developed spontaneously beating heart chambers exhibiting key characteristics of native heart for the first time, which is truly novel and powerful for answering questions that cannot easily be approached in vivo. She has also developed a uniaxial and a biaxial stretching device, which can be used to study the impact of mechanical stimulation on engineered cardiac tissues. More importantly, she has developed a novel flow bioreactor, which allows culture of microvasculature in vitro under the influence of flow, which is critical for any functional tissues. To the best of her knowledge, this was the first gel-based flow bioreactor, which provide a new basis for subsequent co-culture studies with various cell types to develop complex engineered tissue constructs with vascularization capacity, which is extremely critical for ...
A common design constraint in functional tissue engineering is that scaffolds intended for use in load-bearing sites possess similar mechanical properties to the replaced tissue. Here, we tested the hypothesis that in vivo loading would enhance bone morphogenetic protein-2 (BMP-2)-mediated bone regeneration in the presence of a load-bearing PLDL scaffold, whose pores and central core were filled with BMP-2-releasing alginate hydrogel. First, we evaluated the effects of in vivo mechanical loading on bone regeneration in the structural scaffolds. Second, we compared scaffold-mediated bone regeneration, independent of mechanical loading, with alginate hydrogel constructs, without the structural scaffold, that have been shown previously to facilitate in vivo mechanical stimulation of bone formation.. Contrary to our hypothesis, mechanical loading had no effect on bone formation, distribution, or biomechanical properties in structural scaffolds. Independent of loading, the structural scaffolds ...
Background: Cell-based tissue engineering represents a promising management for meniscus repair and regeneration. The present study aimed to investigate whether the injection of parathyroid hormone (PTH) (1-34) could promote the regeneration and chondroprotection of 3D printed scaffold se...
Skeletal muscle plays an important role in the bodys physiology but there are still no effective treatments for volumetric muscle loss (VML) resulting from severe traumatic injury or tumor excision. Recent studies show that a tissue engineering strategy using a compound containing mesenchymal stem cells (MSCs) and decellularized extracellular matrix (ECM) scaffold generates significant regenerative effects on VML injury, but the underlying mechanisms are not fully understood. The characteristics of human umbilical cord MSCs, including multiplication capacity and multidifferentiation ability, were determined. We constructed a compound containing MSCs and decellularized ECM scaffold which was used for tissue regeneration in a VML model. We found that MSCs and decellularized ECM scaffold generated synergistic effects on promoting skeletal muscle tissue regeneration. Interestingly, both MSCs and decellularized ECM scaffold could promote macrophage polarization toward the M2 phenotype and suppress
TY - JOUR. T1 - Optimizing the medium perfusion rate in bone tissue engineering bioreactors. AU - Grayson, Warren L. AU - Marolt, Darja. AU - Bhumiratana, Sarindr. AU - Fröhlich, Mirjam. AU - Guo, X. Edward. AU - Vunjak-Novakovic, Gordana. PY - 2011/5. Y1 - 2011/5. N2 - There is a critical need to increase the size of bone grafts that can be cultured in vitro for use in regenerative medicine. Perfusion bioreactors have been used to improve the nutrient and gas transfer capabilities and reduce the size limitations inherent to static culture, as well as to modulate cellular responses by hydrodynamic shear. Our aim was to understand the effects of medium flow velocity on cellular phenotype and the formation of bone-like tissues in three-dimensional engineered constructs. We utilized custom-designed perfusion bioreactors to culture bone constructs for 5 weeks using a wide range of superficial flow velocities (80, 400, 800, 1,200, and 1,800μm/s), corresponding to estimated initial shear stresses ...
Tissue Engineering[edit]. Melt electrospinning is used to process biomedical materials for tissue engineering research. ... Dalton PD, Jörgensen N, Groll J, Möller M (2008) Patterning of melt electrospun substrates for tissue engineering. Biomed Mater ... Not using solvents to process a polymer assists in tissue engineering applications where solvents are often toxic. Additionally ... Melt electrospun fibers were used as part of a "bimodal tissue scaffold", where both micron-scale and nano-scale fibers were ...
Tissue engineering[edit]. This is mainly concerned with the replacement of tissues which have been destroyed by sickness or ... Biosensors, tissue engineering, drug delivery, or enzymatic catalysis is just a few of the possible examples. The incorporation ... For a broad range of applications including catalysis, tissue engineering, and surface modification of implants this infinite ... "Tungsten disulfide nanotubes reinforced biodegradable polymers for bone tissue engineering". Acta Biomaterialia. 9 (9): 8365-73 ...
Tissue engineering[edit]. Nanotechnology may be used as part of tissue engineering to help reproduce or repair or reshape ... Tissue engineering if successful may replace conventional treatments like organ transplants or artificial implants. ... "Tungsten disulfide nanotubes reinforced biodegradable polymers for bone tissue engineering". Acta Biomaterialia. 9 (9): 8365-73 ... "Two-dimensional nanostructure-reinforced biodegradable polymeric nanocomposites for bone tissue engineering". Biomacromolecules ...
"Ligament Tissue Engineering and Its Potential Role in Anterior Cruciate Ligament Reconstruction". Stem Cells International. ... Autografts (employing bone or tissue harvested from the patient's body). *Allografts (using bone or tissue from another body, ... Synthetic tissue for ACL reconstruction has also been developed, but little data exists on its strength and reliability.[ ... Anterior cruciate ligament reconstruction (ACL reconstruction) is a surgical tissue graft replacement of the anterior cruciate ...
... ex vivo engineering of living tissues with adult stem cells". Tissue Engineering. 12 (11): 3007-19. doi:10.1089/ten.2006.12. ... Yen AH, Sharpe PT (January 2008). "Stem cells and tooth tissue engineering". Cell and Tissue Research. 331 (1): 359-72. doi: ... "Advances in Biochemical Engineering/Biotechnology. Advances in Biochemical Engineering/Biotechnology. 114: 185-99. Bibcode: ... "Genetic Engineering & Biotechnology News. Mary Ann Liebert, Inc. p. 13. Retrieved 2008-07-06. (subtitle) Procymal is being ...
Tissue engineering. 11 (1): 1-18. doi:10.1089/ten.2005.11.1. PMID 15738657.. ... Foo, K. Y.; Hameed, B. H. (2010). "Insights into the modeling of adsorption isotherm systems". Chemical Engineering Journal. ... Industrial and Engineering Chemistry Research. 37 (6): 2239-2245. doi:10.1021/ie970696d.. ...
"Journal of Tissue Engineering. 8: 2041731417731546. doi:10.1177/2041731417731546. PMC 5624345. PMID 28989698.. ...
"Tissue Engineering Part B: Reviews. 17 (4): 249-262. doi:10.1089/ten.TEB.2011.0040. PMC 3142632. PMID 21491967.. ... "Tissue Engineering Part A. 16 (2): 629-641. doi:10.1089/ten.tea.2009.0458. PMC 2813151. PMID 20001738.. ... Tissue Engineering Part A. 17 (15-16): 1901-1909. doi:10.1089/ten.TEA.2010.0563. PMID 21417693.. ... Tissue Engineering Part A. 15 (2): 331-342. doi:10.1089/ten.tea.2008.0145. PMID 19193130.. ...
Yen AH, Sharpe PT (January 2008). "Stem cells and tooth tissue engineering". Cell Tissue Res. 331 (1): 359-72. doi:10.1007/ ... In the case of wounded fetal tissue, however, wounded tissue is replaced with normal tissue through the activity of stem cells. ... tissue-engineered bone regeneration". Tissue Eng. 10 (5-6): 955-64. doi:10.1089/1076327041348284. PMID 15265313.. ... "Stem cell-based tissue engineering in veterinary orthopaedics". Cell Tissue Res. 347 (3): 677-88. doi:10.1007/s00441-011-1316-1 ...
Journal of Tissue Engineering and Regenerative Medicine. 13 (2): 261-273. doi:10.1002/term.2789. PMID 30554484.. ... PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent ... During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in ... The first engineered dominant negative protein was designed to inhibit PDGF [29] ...
... ex vivo engineering of living tissues with adult stem cells". Tissue Eng. 12 (11): 3007-3019. doi:10.1089/ten.2006.12.3007. ... Journal of Tissue Engineering and Regenerative Medicine. 1 (1): 74-79. doi:10.1002/term.8. PMID 18038395.. ... MSCs have been isolated from placenta, adipose tissue, lung, bone marrow and blood, Wharton's jelly from the umbilical cord,[23 ... Pluripotent stem cells, i.e. cells that can give rise to any fetal or adult cell type, can be found in a number of tissues, ...
Journal of Tissue Engineering and Regenerative Medicine. 9 (5): 528-39. doi:10.1002/term.1957. PMID 25370612.. ... tissue homeostasis. • heart process. • positive regulation of cardiac muscle cell proliferation. • cardiac muscle tissue ... It is reported that several genes are regulated by YAP1, including Birc2, Birc5, connective tissue growth factor (CTGF), ...
Lavik, E. and R. Langer, Tissue engineering; current state and perspectives. Applied Microbiology Biotechnology, 2004. 65: p. 1 ... Rectal mucosa is highly vascularized tissue that allows for rapid and effective absorption of medications.[15] A suppository is ... This is the most reliable route, as in acutely ill patients the absorption of substances from the tissues and from the ...
Research has also been conducted into the engineering of heart valves. Tissue-engineered heart valves derived from human cells ... In humans with non-injured tissues, the tissue is naturally regenerated over time; by default these tissues have new cells ... Indeed, in 2008, there was a successful clinical transplantation of a tissue-engineered trachea in a 30-year-old woman with end ... 2010). "Tissue-Engineered Lungs for in Vivo Implantation". Science. 329: 538-541. doi:10.1126/science.1189345. Bonvillain RW, ...
... can also be used for medical purposes.[51] The electrospun scaffolds made for tissue engineering applications ... This early application of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types ... Sill, Travis J.; von Recum, Horst A. (May 2008). "Electrospinning: Applications in drug delivery and tissue engineering". ... A novel scaffold for tissue engineering". Journal of Biomedical Materials Research. 60 (4): 613-621. doi:10.1002/jbm.10167.. ...
... making HA very suitable for tissue-engineering studies. For example, HA hydrogels are appealing for engineering vasculature ... Zheng Shu X, Liu Y, Palumbo FS, Luo Y, Prestwich GD (2004). "In situ crosslinkable hyaluronan hydrogels for tissue engineering ... Granulation tissue is the perfused, fibrous connective tissue that replaces a fibrin clot in healing wounds. It typically grows ... Cell migration is essential for the formation of granulation tissue.[18] The early stage of granulation tissue is dominated by ...
"Tissue Engineering Part A. 20 (17-18): 2305-2315. doi:10.1089/ten.tea.2013.0328. PMC 4172384. PMID 24593020.. ... "Porous three-dimensional carbon nanotube scaffolds for tissue engineering". Journal of Biomedical Materials Research Part A. ... "Collagen-carbon nanotube composite materials as scaffolds in tissue engineering". Journal of Biomedical Materials Research Part ... Their potential and pitfalls for bone tissue regeneration and engineering". Nanomedicine: Nanotechnology, Biology and Medicine ...
... on chondrocytes has shown potential as a means to produce therapeutic cellular biomaterials via tissue engineering and ... bone and connective tissues. Overwhelming disorganization of cellular processes involved in the formation of cartilage and bone ... specialized cells that make up fibrous connective tissue, which plays a role in the formation of cellular structure and ... causing abnormal fibrous development of cartilage and related tissues. It is a lethal rhizomelic (malformations which result in ...
Techniques such as the EELS-TALC to enhance ACI and MACI with enabling chondrocytes to be tissue engineered with long term ... with the engineered tissue construct containing stem cell progenitors along with those expressing pluripotency markers and with ... Tissue Engineering. 12 (5). PMID 16771637. Arumugam, S (2007). "Transplantation of autologous chondrocytes ex-vivo expanded ... This drives efforts to develop ways of using a person's own cells to grow, or re-grow cartilage tissue to replace missing or ...
Tissue Engineering. 13 (10): 2431-40. doi:10.1089/ten.2006.0406. PMC 2835465. PMID 17630878. Tu Q, Valverde P, Chen J (March ... During development, a mouse embryo model with Sp7 expression knocked out had no formation of bone tissue. Through the use of ... Calcified Tissue International. 78 (2): 98-102. doi:10.1007/s00223-005-0146-0. PMID 16467978. S2CID 7621703. Wu L, Wu Y, Lin Y ... a severe phenotype in which there were unaffected chondrocytes and cartilage but absolutely no formation of bone tissue. ...
Charles Vacanti; Anasthesiologist; Tissue engineering; Stem Cells; Known for the Vacanti Mouse; Uxbridge has a Board of ... "West Hill Dam, Uxbridge Massachusetts". US Army Corps of Engineers. Archived from the original on October 1, 2007. Retrieved ...
Biomimetic approach to cardiac tissue engineering: oxygen carriers and channeled scaffolds. Tissue Engineering, 12(8), pp. 2077 ... American Institute for Medical and Biological Engineering as well as Tissue Engineering and Regenerative Medicine Society. ... She also researched on the biometric cues in vitro and developed an engineered oriented cardiac tissue. Radisic has also worked ... Milica Radisic is a Serbian Canadian tissue engineer, academic and researcher. She is a Professor at the University of ...
Bhatia co-authored the first undergraduate textbook on tissue engineering, Tissue engineering (2004), written for senior-level ... Bhatia, S. N.; Underhill, G. H.; Zaret, K. S.; Fox, I. J. (16 July 2014). "Cell and tissue engineering for liver disease". ... Palsson, Bernhard Ø.; Bhatia, Sangeeta N. (2004). Tissue engineering. Upper Saddle River, N.J.: Pearson Prentice Hall. Nahmias ... Palsson, Bernhard Ø.; Bhatia, Sangeeta N. (2004). Tissue engineering. Upper Saddle River, N.J.: Pearson Prentice Hall. Nahmias ...
Tissue Engineering. 13 (11): 2681-7. doi:10.1089/ten.2006.0447. PMID 17691866. Chachques JC, Azarine A, Mousseaux E, El Serafi ... which has since advanced into the exciting realms of tissue engineering science. In 2008, Carpentier announced a fully ... The prototype uses electronic sensors and is made from chemically treated animal tissues, called "biomaterials," or a "pseudo- ...
After 20 days of perfusion with growth factors, the engineered heart tissues started to beat again and were responsive to drugs ... Therefore, there is an urgent need for effective cell therapy and lung tissue engineering. Several protocols have been ... Tissue Engineering Part A. 16 (4): 1363-1368. doi:10.1089/ten.tea.2009.0339. PMC 2862604. PMID 19912046. Wu, X., Wang, S., Li, ... Tissue Engineering Part B: Reviews. 20 (4): 257-66. doi:10.1089/ten.teb.2012.0757. PMC 4123466. PMID 23957872. Outani, H.; ...
... tissue engineering, implants and more. Tissue Engineering Substrates Polyethyleneterephthalate (PET) cell adhesion Improved ... Tissue Engineering. Part B, Reviews. 24 (5): 359-372. doi:10.1089/ten.TEB.2018.0056. ISSN 1937-3376. PMC 6199621. PMID 29631491 ...
Tissue Engineering. Part A. 23 (11-12): 535-545. doi:10.1089/ten.TEA.2016.0494. PMC 5467120. PMID 28125933. Mistriotis P, ...
Tissue Engineering. Part A. 15 (8): 1897-1907. doi:10.1089/ten.tea.2008.0351. PMC 2792108. PMID 19196144. Kumar, Rani (2008). ... The correct specification of the deriving tissues, skeletal, cartilage, endothelia and connective tissue is achieved by a ... Somitic derivatives are determined by local signaling between adjacent embryonic tissues, in particular the neural tube, ... ISBN 978-0-632-02182-6. Liu, Shu Q. (2007). "Early Embryonic Organ Development". Bioregenerative engineering: principles and ...
Adipose tissue secretes the hormone leptin, and leptin suppresses appetite. Long-term satiety signals from adipose tissue ... Sharia and Social Engineering: p 143, R. Michael Feener - 2013 *^ FOOD & EATING IN MEDIEVAL EUROPE - Page 73, Joel T. Rosenthal ... The long-term signals of satiety come from adipose tissue.[20] The taste and odor of food can contribute to short-term satiety ... Long-term satiety comes from the fat stored in adipose tissue. ...
"Detritus can be broadly defined as any form of non-living organic matter, including different types of plant tissue (e.g. leaf ... Many of the Earth's elements and minerals (or mineral nutrients) are contained within the tissues and diets of organisms. Hence ... litter, dead wood, aquatic macrophytes, algae), animal tissue (carrion), dead microbes, faeces (manure, dung, faecal pellets, ...
Keratinocytes engineered to not express alpha-catenin have disrupted cell adhesion and activated NF-κB. A tumor cell line with ... Mice lacking plakoglobin have cell adhesion defects in many tissues, although β-catenin substitutes for plakoglobin at many ... Mice engineered to specifically have vascular endothelium cells deficient in β-catenin showed disrupted adhesion between ... F9 cells were genetically engineered to lack β-catenin, resulting in increased association of plakoglobin with E-cadherin. In ...
A CT scan can distinguish muscle tissue from other tissues and thereby estimate the amount of muscle tissue in the body. Fast ... D.Zhang et al., Functional Electrical Stimulation in Rehabilitation Engineering: A survey, Nenyang technological University, ... Disuse of the muscles, such as when muscle tissue is immobilized for even a few days of unuse - when the patient has a primary ... There are a few known factors that contribute to the sustaining of muscle tissue. During the summer period, bears take ...
"Challenges in Cardiac Tissue Engineering". Tissue Engineering Part B: Reviews 16 (2): 169-187. doi:10.1089/ten.teb.2009.0352. ...
Once the shell is penetrated, the prey dies almost instantaneously, its muscles relax, and the soft tissues are easy for the ... IEEE Engineering in Medicine and Biology Society. 25 (3): 40-56. doi:10.1109/MEMB.2006.1636351.. ... The skin consists of a thin outer epidermis with mucous cells and sensory cells, and a connective tissue dermis consisting ... Extensive connective tissue lattices support the respiratory muscles and allow them to expand the respiratory chamber.[37] The ...
Biomedical Engineering is a field dealing with the application of engineering principles to medical practice. ... Histology is the study of the structures of biological tissues by light microscopy, electron microscopy and ... the knowledge of what happens at the cellular and molecular level in the tissues being stitched arises through science. ...
Ultimately, the connectivity (and consequently the functionality) of the tissue is changed from what the original template ... Artificial life Artificial neural networks Brain-computer interface CoDi Cybernetics Neural ensemble Neural engineering ... IEEE Transactions on Biomedical Engineering. 55 (4): 1382-1390. doi:10.1109/TBME.2007.913987. PMID 18390329. "Axion MEA Systems ...
Shroy Jr RE (1995). "X-Ray equipment". In Bronzino JD (ed.). The Biomedical Engineering handbook. CRC Press and IEEE Press. pp ... Fluoroscopy is mainly performed to view movement (of tissue or a contrast agent), or to guide a medical intervention, such as ... while it could pass through human tissue, it could not pass through bone or metal.[24] Röntgen referred to the radiation as "X ... and engineers. The medical speciality of radiology grew up over many years around the new technology. When new diagnostic tests ...
"Cesium and Rubidium Hit Market". Chemical & Engineering News. 37 (22): 50-56. 1959. doi:10.1021/cen-v037n022.p050.. ... organ changes and tissue accumulation". Journal of Environmental Science and Health, Part A. 16 (5): 549-567. doi:10.1080/ ... "Chemical and Engineering News. 81 (36): 159. doi:10.1021/cen-v081n036.p159.. ... Lindsey, Jack L (1997). Applied illumination engineering. p. 112. ISBN 978-0-88173-212-2. .. ...
2008). "Monitoring Tissue Engineering Using Magnetic Resonance Imaging". Journal of Bioscience and Bioengineering. 106 (6): 515 ... Connective tissue (CT) is one of the four basic types of animal tissue, along with epithelial tissue, muscle tissue, and ... and special connective tissue.[5][6] Connective tissue proper consists of loose connective tissue and dense connective tissue ( ... Special connective tissue consists of reticular connective tissue, adipose tissue, cartilage, bone, and blood.[8] Other kinds ...
"Feet of Engineering." 99% Invisible. Jun 2014. *^ a b c d e Morris, Paul; Jenny White; Edward Morrison; Kayleigh Fisher (May ... decreased muscle movement and even tissue deformation. ...
Engineering modifications have added hand-controlled levers to the LFC, to enable users to move the chair over uneven ground ... the parts of the body that are the most at risk for tissue breakdown include the ischial tuberosities, coccyx, sacrum and ... The chair has been engineered to be low-cost and usable on the rough roads common in developing countries. ... and his disabled friend Herbert Everest, both mechanical engineers, invented the first lightweight, steel, folding, portable ...
Initially, moving organisms such as sharks and hagfish, scavenge the soft tissues at a rapid rate over a period of months, and ... "Whales as marine ecosystem engineers". Frontiers in Ecology and the Environment. 12 (7): 377-385. doi:10.1890/130220.. ... These vibrations are received through fatty tissues in the jaw, which is then rerouted into the ear-bone and into the brain ... In addition to their streamlined bodies, they can slow their heart rate to conserve oxygen; blood is rerouted from tissue ...
IEEE Engineering in Medicine and Biology Society. Annual Conference. 2011. pp. 8408-11. doi:10.1109/IEMBS.2011.6092074. ISBN ... First, an infected tissue sample is taken from the patient. Then an oligonucleotide complementary to the suspected pathogen's ... Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. ... The tissue sample is chemically treated in order to make the cell membranes permeable to the fluorescently tagged ...
Bazin, Hervé (2011). Vaccination: a history from Lady Montagu to genetic engineering. Montrouge: J. Libbey Eurotext. p. 407. ... A French team developed the French neurotropic vaccine (FNV), which was extracted from mouse brain tissue. Since this vaccine ... reduced tissue damage in the liver and spleen, prevented hepatocellular steatosis, and normalised levels of alanine ...
Biomaterials science encompasses elements of medicine, biology, chemistry, tissue engineering, and materials science. ... chemical engineering, mechanical engineering, and electrical engineering; and more. ... Askeland, Donald R.; Pradeep P. Phulé (2005). The Science & Engineering of Materials (5th ed.). Thomson-Engineering. ISBN 978-0 ... The field of materials science and engineering is important both from a scientific perspective, as well as from an engineering ...
Engineers. and inventors. *William Beebe. *Georges Beuchat. *John R. Clarke. *Jacques Cousteau ...
Investigative teams recovered duct tape which was hanging from Caylee's hair and some tissue left on her skull.[4] Over the ... a retired engineer and computer expert in Connecticut, revealed that her password-protected computer account shows activity on ...
Luciferase systems are widely used in the field of genetic engineering. They have also been used in biomedical research, to ... Usually these light organs are separate from the tissue containing the bioluminescent bacteria. ...
Genetic engineering[edit]. TATA box modification[edit]. Evolutionary changes have pushed plants to adapt to the changing ... "Polymorphisms in the 5' regulatory region of the tissue factor gene and the risk of myocardial infarction and venous ...
Caye Drapcho; Nhuan Phú Nghiêm; Terry Walker (August 2008). Biofuels Engineering Process Technology. [McGraw-Hill]. ISBN 978-0- ... Escherichia coli strains have also been successfully engineered to produce butanol by modifying their amino acid metabolism.[36 ... Li, H.; Cann, A. F.; Liao, J. C. (2010). "Biofuels: Biomolecular Engineering Fundamentals and Advances". Annual Review of ... Biomass and Alternate Fuel Systems: An Engineering and Economic Guide. ISBN 978-0-470-41028-8 Wiley, 280 pages ...
Jagadeeswara Raoa; K.A. Venkatesana; K. Nagarajana; T.G. Srinivasan & P.R. Vasudeva Rao (2007). "Treatment of tissue paper ... Zhou, Feng; Liang, Yongmin; Liu, Weimin (2009-08-19). "Ionic liquid lubricants: designed chemistry for engineering applications ... The dissolution of cellulose-based materials like tissue paper waste, generated in chemical industries and at research ...
Doble, Mukesh; Gummadi, Sathyanarayana N. (August 5, 2010). Biochemical Engineering. New Delhi: Prentice-Hall of India Pvt.Ltd ... where specialized cells of the same type aggregate to form tissues, then organs and ultimately systems.[12] The G phases along ...
"Engineers resolve Orion will 'lose weight' in 2015". NASA. 16 January 2013. Retrieved 22 March 2013.. This article incorporates ... which will measure tissue radiation dose deposition and test the effectiveness of the AstroRad radiation vest in the radiation ... "Engineers resolve Orion will 'lose weight' in 2015". WAFF. 13 January 2015. Retrieved 15 January 2015.. ... measure radiation exposure not only at the surface of the body but also at the exact location of sensitive organs and tissues ...
The brain type is predominant in adult brain and embryonic tissues, whereas the liver and muscle types are predominant in adult ... Coats WS, Browner MF, Fletterick RJ, Newgard CB (Aug 1991). "An engineered liver glycogen phosphorylase with AMP allosteric ... David ES, Crerar MM (January 1986). "Quantitation of muscle glycogen phosphorylase mRNA and enzyme amounts in adult rat tissues ... all stemming from low glucose levels in muscle tissue.[17] ...
These compounds are stored in the body's fat, and when the fatty tissues are used for energy, the compounds are released and ...
GAG - gamma globulin - gamma interferon - ganglion - GART - gastrointestinal (GI) - gene - gene therapy - genetic engineering ... tissue - titer - toxicity - toxoplasmic encephalitis - toxoplasmosis - transaminase - transcription - transfusion - translation ... lymphoid tissue - lymphokine-activated killer cells (LAK) - lymphokines - lymphoma - lymphopenia - lymphoproliferative response ...
"Stem cells and tooth tissue engineering". Cell Tissue Res. 331 (1): 359-72. 2008. doi:10.1007/s00441-007-0467-6. PMID 17938970. ... Genetic Engineering & Biotechnology News (Mary Ann Liebert, Inc.), 2008-06-15. 13. lpp. Skatīts: 2008-07-06. (subtitle) ... Advances in Biochemical Engineering/Biotechnology 114: 185-99. 2009. Bibcode doi:10.1007/10_2008_45. ISBN ... "A hypothesis for an embryonic origin of pluripotent Oct-4(+) stem cells in adult bone marrow and other tissues". Leukemia 21 (5 ...
... Cross-section of a lamprey spinal cord stained with ... The Eugene Bell Center for Regenerative Biology and Tissue Engineering was established in 2010 through the extraordinary ... Tissue-specific gene inactivation in Xenopus laevis: knockout of lhx1 in the kidney with CRISPR/Cas9. Genetics 208(2): 673-686 ... genetic and cellular mechanisms underlying the growth and replacement of highly differentiated tissues during development, ...
The Cartilage Tissue Engineering Laboratory in the Department of Bioengineering at the University of California, San Diego was ... We are members of the Institute of Engineering in Medicine and also the UCSD Stein Institute for Research on Aging.. ... We collaborate with several groups at UCSD in the Department of Bioengineering within the Jacobs School of Engineering, as well ...
Experimental Medicine and Biology includes much of the research presented at the recent Second International Tissue Engineering ... This issue should be a useful reference for tissue engineering courses as well as for researchers developing engineered tissues ... Evaluation of Various Types of Scaffold for Tissue Engineered Intervertebral Disc Soon Hee Kim, Sun Jung Yoon, Bangsil Choi, ... Injectable Synthetic Extracellular Matrices for Tissue Engineering and Repair Glenn D. Prestwich, Xiao Zheng Shu, Yanchun Liu, ...
Tissue Engineering at, the leading libertarian magazine and video website covering news, politics, culture, science ...
The term tissue engineering was introduced in the late 1980s. By the early 1990s the concept of applying engineering to the ... Tissue engineering, scientific field concerned with the development of biological substitutes capable of replacing diseased or ... tissue engineering; regenerative medicineA section of tissue engineered to serve as a vascular graft.. HIA. ... Examples of tissues that are candidates for tissue engineering include skin, cartilage, heart, and bone. The production of skin ...
... cirtique of tissue engineered products skin, cardiovascular substitutes, cartilage, bone, ligaments/tendons Tissue Engineering ... bioreactors for tissue engineered constructs, preservation of tissue engineered constructs Clinical applications - anatomy, ... Construct Technology 3-D structure, multi-cellular systems, transport of nutrients and metabolites in tissue engineering, ... models for tissue engineering, physical characteristics for transplantation, cell function in constructs; influence of ...
The advancements in scaffold-supported cell therapy for musculoskeletal tissue engineering have been truly dramatic in the last ... the integration of tissue-engineered "biological implants" with the native tissue. All these developments in regenerative ... Hydrogels Minimally invasive Musculoskeletal tissue engineering Scaffold Stem cell Abbreviations. ACT. Autologous Chondrocyte ... The advancements in scaffold-supported cell therapy for musculoskeletal tissue engineering have been truly dramatic in the last ...
This session will have presentations on the engineering of skeletal tissue, with topics such as the design of materials that ... 55f) Understanding the Tissue Growth Process Via Fluid Shear and Nutrient Transport Simulation in 3D Porous Scaffolds Used in a ... AI For Chemical Engineers: Fast Track Your Journey to Get Ahead March 24, 2021. ... AIChE Engage connects AIChE members with each other and their chemical engineering communities. ...
Now indexed in PubMed/MEDLINEWe are pleased to announce that Tissue Engineering and Regenerative Medicine has been accepted for ...
Studies in Mechanobiology, Tissue Engineering and Biomaterials. Series Volume. 6. Copyright. 2011. Publisher. Springer-Verlag ... Myocardial tissue engineering (MTE), a strategy that uses materials or material/cell constructs to prolong patients life after ... Intramyocardial Stem Cell Transplantation Without Tissue Engineered Constructs: The Current Clinical Situation ... Common MTE strategies include an engineered vehicle, which may be a porous scaffold or a dense substrate or patch, made of ...
... and the use of such an implant as a scaffold for tissue-engineering and as a transplant tissue in reconstructive or replacement ... The invention provides a process for the production of a monolithic implant for use in tissue engineering, the process ... maintain and improve tissue functions. Tissue engineering can be applied to both hard and soft tissues. Hard tissue includes, ... Tissue Engineering Scaffolds. * [0101] Extrusion of monolithic structures can be applied to tissue engineering scaffolds for ...
Purchase Developmental Biology and Musculoskeletal Tissue Engineering - 1st Edition. Print Book & E-Book. ISBN 9780128114674, ... Discusses the role of genes in the development of musculoskeletal tissues and their potential use in tissue engineering ... Recent developments in orthopedic tissue engineering have sought to recapitulate developmental processes for tissue repair and ... Developmental biology of musculoskeletal tissues for Tissue Engineers. 2. The Mechanics of Musculoskeletal Development. 3. ...
Purchase Biomedical Foams for Tissue Engineering Applications - 1st Edition. Print Book & E-Book. ISBN 9780857096968, ... which are increasingly being used for tissue engineering applications. Biomedical Foams for Tissue Engineering Applications ... biomedical foams for tissue engineering, porous hydrogel biomedical foam scaffolds for tissue repair, and titanium biomedical ... Biomedical Foams for Tissue Engineering Applications is a technical resource for researchers and developers in the field of ...
Tissue Engineering[/I] brings together scientific and medical experts in the fields of biomedical engineering, material science ... Tissue Engineering, Part A (image). Mary Ann Liebert, Inc./Genetic Engineering News ... Tissue Engineering brings together scientific and medical experts in the fields of biomedical engineering, material science, ... molecular and cellular biology, and genetic engineering. ... Mary Ann Liebert, Inc./Genetic Engineering News. Journal. ...
... of engineering and life sciences to advance the field with cutting-edge research and applications on all aspects of tissue ... Tissue Engineering, Part A Tissue Engineering, Part A Published 24 times per year, the flagship provides a fundamental ... Tissue Engineering, Part B: Reviews Tissue Engineering, Part B: Reviews This bimonthly complement to the flagship presents ... Tissue Engineering, Part C: Methods Tissue Engineering, Part C: Methods Presents procedures and protocols of research methods ...
... the Laboratory for Tissue Engineering and Organ Fabrication designs and builds organs and tissues using cells combined with ... Tissue Engineering is a new field in science, medicine and engineering in which living replacements for organs and tissues of ... Liver Tissue Engineering. Extensive work has been devoted to developing a tissue engineered liver. This liver is comprised of ... Lung Tissue Engineering. Efforts to develop a tissue engineered lung have only recently begun to gain momentum despite a ...
How good must cartilage repair be for it to be worthwhile? What is the best source of cells for tissue engineering of both bone ... tissue engineering was driven by material scientists who designed novel bio-resorbable scaffolds on which to seed cells and ... this timely publication will prove essential reading for anyone with an interest in the field of tissue engineering. ... grow tissues. This ground-breaking work generated high expectations, but there have been significant stumbling blocks holding ...
14:00 CARDIOVASCULAR TISSUE ENGINEERING. Dr Mark Eastwood, Reader in Biomedical Science, Centre for Tissue Engineering Research ... Valuation of tissue engineering companies *Attractiveness of tissue engineering companies to investors *Impact on strategy and ... Tissue Engineering, Smith & Nephew. *Technologies used for production of tissue engineered skin substitutes *Specific ... 15:40 COLLAGEN BIOMATERIALS IN TISSUE REPAIR AND TISSUE ENGINEERING. Dr Jean-Louis Tayot, Chief Scientific Officer, Imedex. * ...
... properties of musculofascial tissues have great importance in musculofascial tissue engineering. However, detailed ... Decellularized musculofascial extracellular matrix for tissue engineering.. Wang L1, Johnson JA, Chang DW, Zhang Q. ... tissue repair and regeneration but also a useful standard for scaffold design in musculofascial tissue engineering. ... Evaluation of cellular components removal from musculofascial tissues. (A) Images of muscle and fascial tissue after ...
9 billion/year market for tissue engineering products finds that 21 of 49 companies in the segment are responsible for the ... Tissue Engineering Market in the U.S.. In the $9 billion/ year market for tissue engineering products, 21 of 49 companies ... Since the term tissue engineering was coined in 1993, the fields of tissue engineering, regenerative medicine, and cell therapy ... This report seeks to provide an update of the tissue engineering industry from 2011 to 2018. Public tissue engineering ...
... tissue engineering firms are inspiring optimistic growth projections. Especially celebrated are the firms bringing injectable ... Tissue-engineered heart vesicles are heart valves grown in specially designed bioreactors and are composed of biological ... CRISPR-Cas genome editing can be used to modify cells to make them more potent for application in tissue engineering. ... Stratistics MRC ( reports that the global tissue engineering market is expected to grow from $7.06 billion ...
Engineering New Tissue for Pediatric Heart Surgery. Bioengineering professor explores potential to grow cardiac cells to repair ... Jacots endothelial cell work was published last June in Tissue Engineering.. Jacot is conducting his research under an NSF ... The National Science Foundation- (NSF) funded scientist is designing new tissue engineering approaches with the aim of making ... Both will go into making cardiac engineered heart tissues, but we turn them into different cell types. ...
... of tissue produce fully functional livers.Many diseases, including cirrhosis and hepatitis, can lead to liver failure. ... "Engineered liver tissue expands after transplant." Medical News Today. MediLexicon, Intl., 24 Jul. 2017. Web.. 10 Dec. 2017. , ... Using tissue engineering, researchers show a way forward for treating type 1 diabetes by implanting insulin-producing islet ... In 2011, she developed an engineered tissue scaffold, about the size and shape of a contact lens, that could be implanted into ...
... engineers, academic, scientific and university practitioners to present research activities that might want to attend events, ... Tissue Engineering Conferences in 2022 is for the researchers, scientists, scholars, ... Tissue Engineering. Tissue Engineering Conferences in 2022. Tissue Engineering Conferences in 2022 is an indexed listing of ... ICTE 2022: Tissue Engineering Conference, Baku (Oct 04-05, 2022) * ICTEDI 2022: Tissue Engineering and Dental Implants ...
... Incidences of bone disorders constitute a significant economic burden to societies globally. In the ... To address those disadvantages, bone tissue engineering has emerged as an alternative regenerative strategy. ... "Developing New Tissue Engineering Technology for Bone Implants". The grant includes $100,000 for undergraduate research ... Current approaches for replacing the damaged bone tissues include the use of bone grafts (ie, auto-grafts or allografts). ...
Tissue engineering promotes regrowth of cells lost to trauma or disease. The McGowan Institute is a pioneer in the development ... Tissue Engineering. The term tissue engineering refers to methods that promote the regrowth of cells lost to trauma or ... Whole Organ Engineering. Organ engineering, as opposed to tissue engineering, poses significant challenges including the ... Tissue engineers use many methods, in​cluding the manipulation of artificial and natural materials that provide structure and ...
Japans largest platform for academic e-journals: J-STAGE is a full text database for reviewed academic papers published by Japanese societies
Information about the open-access journal AIMS Cell and Tissue Engineering in DOAJ. DOAJ is an online directory that indexes ...
Tissue engineering of temporomandibular joint cartilage. [K A Athanasiou;] -- The temporomandibular joint (TMJ) is a site of ... Central to TMJ afflictions are the cartilaginous tissues of the TMJ, ... ... Tissue engineering of the disc --. Introduction --. Previous tissue engineering efforts --. Scaffolds --. Bioactive agents --. ... Mandibular condyle tissue engineering studies --. Current perspectives --. Cell sources for tissue engineering of cartilage -- ...
  • The Eugene Bell Center for Regenerative Biology and Tissue Engineering was established in 2010 through the extraordinary leadership gifts of Millicent Bell and John and Valerie Rowe. (
  • We work closely with stem cell biologists, material scientists and engineers from the Center for Regenerative Medicine, MIT, Brigham and Women's Hospital and the Draper Laboratories. (
  • Since the term tissue engineering was coined in 1993, the fields of tissue engineering, regenerative medicine, and cell therapy have greatly matured from benchtop ideas to commercially available products that are widely used in the clinic. (
  • Currently, the terms "tissue engineering" and "regenerative medicine" bring up 1.2 and 0.6 million results on Google Scholar, respectively. (
  • To address those disadvantages, bone tissue engineering has emerged as an alternative regenerative strategy. (
  • Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends," Journal of the Royal Society Interface , vol. 4, no. 17, pp. 999-1030, 2007. (
  • Tissue engineering is considered one of the most important therapeutic strategies of regenerative medicine. (
  • Creating an organ - Dr. Yuanyuan Zhang, assistant professor at Wake Forest Institute for Regenerative Medicine, demonstrates the process used to engineer a vaginal organ. (
  • Key biomaterials focussed activities in the Department of Materials include the development of new scaffolds for regenerative medicine, biomaterials characterisation, stem cell therapy, cell-materials interface engineering, self-assembled biomimetic copolymers and nanomaterials for biosensing applications. (
  • Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. (
  • In 2013, Methuselah Foundation which is a medical charity announced a million dollar New Organ Liver award to competitors for development over a five-year international competition to advance the field of tissue engineering and regenerative medicine. (
  • It is important to regenerative medicine or tissue repair by using a tissue scaffold for the creation of new feasible tissue for medical purposes. (
  • The theme of the 2015 conference will be "Regenerative Engineering and Functional Materials Integration" and will feature multidisciplinary presentations by bioengineers, chemists and clinical scientists on emerging topics in immunology and stem cells as well as basic and translational aspects of biomaterials science. (
  • Session topics include: Emerging Concepts in Immunity, Harnessing Inflammation for Tissue Repair, Phenotypic Diversity of Inflammary Response, Therapeutic Neovascularization, Strategies to Direct Neural Regeneration, Harnessing Niche Concepts for Regenerative Medicine, Stimulus Responsive Biomaterials, Emerging Concepts in Regenerative Engineering, and Translational Biomaterials Research. (
  • This GRC will highlight such cutting-edge advances to understand how material properties and material-biomolecule conjugation strategies affect biological activities, and have thereby enabled the application of instructive biomaterials in tissue engineering, regenerative medicine, immune engineering, and diagnostics, to name a few. (
  • However, if the amount of sustained damage exceeds the body's repair capacity, regenerative medicine, including tissue engineering, provides temporary or permanent assistance. (
  • For this reason they are studied as targets and tools for tissue engineering and regenerative medicine, which reiterate a number of these processes. (
  • This chapter highlights current strategies using miRNAs for tissue engineering and regenerative medicine-for example, their influence in of mesenchymal stem cell differentiation to osteoblasts and chondrocytes for bone and cartilage repair as well their role in myogenic differentiation of satellite cells. (
  • The technology helps nudge medical science closer to one day growing human organ tissues from a person's own cells for regenerative therapy, say study investigators at Cincinnati Children's Hospital Medical Center in the U.S. and Yokohama City University (YCU) in Japan. (
  • covers the recent developments, trends and innovations in the application of nanotechnologies in tissue engineering and regenerative medicine. (
  • It is aimed for R&D and academic scientists, lab engineers, lecturers and PhD students engaged in the fields of tissue engineering or more generally regenerative medicine, nanomedicine, medical devices, nanofabrication, biofabrication, nano- and biomaterials and biomedical engineering. (
  • 10 MSCs are easily harvested from the iliac crest and are suitable for regenerative therapies due to their high duplication capacity (up to forty times), ability to withstand preservation by freezing and capacity to build new tissue in a defect. (
  • Other drug therapy revenues (e.g., painkillers, analgesics) that can be used along with regenerative therapy are not included in the report, as they do not contribute to cell or tissue regeneration. (
  • Cellular engineering focuses on cell-level phenomena, while tissue engineering and regenerative medicine seek to generate or stimulate new tissue for disease treatment. (
  • The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues. (
  • Tissue Engineering is a new field in science, medicine and engineering in which living replacements for organs and tissues of the body are designed and built. (
  • The Laboratory for Tissue Engineering and Organ Fabrication at Massachusetts General Hospital has been designing and building organs and tissues for almost 20 years. (
  • Growing usage of tissue engineering as an alternative for transplant organs is the major factor propelling the market's growth. (
  • The main objective of these new technologies is the development of substitutes made with biomaterials that are able to heal, repair or regenerate injured or diseased tissues and organs. (
  • These constructs seek to unlock the limited ability of human tissues and organs to regenerate. (
  • It is not the first time scientists have engineered body parts -- in effect, creating organs where before there were none. (
  • This is a move forward to even more challenging (organs) , " said Ivan Martin, a professor of tissue engineering at University Hospital Basel in Switzerland, and co-author of the nasal cartilage study. (
  • Researchers around the world are making groundbreaking progress in engineering replacement organs. (
  • Since the first successes with bioengineered skin - which can be used for grafts to treat people with burns, for example - tissue engineers have created lab-grown cartilage, bone and, most recently, whole organs such as bladders . (
  • Tissue engineers now hope to bypass lengthy waits for donor organs by using synthetic scaffolds. (
  • The goal of tissue engineering is to repair or replace tissues and organs by delivering implanted cells, scaffolds, DNA, proteins, and/or protein fragments at surgery. (
  • Tissue engineering" uses implanted cells, scaffolds, DNA, protein, and/or protein fragments to replace or repair injured or diseased tissues and organs. (
  • The tissue-engineering process also uses two types of embryonic-stage progenitor cells, which support formation of the body and its specific organs. (
  • Tissue engineering is an interdisciplinary field in which scaffolds, cells, and biologically active molecules are used to construct body tissues and organs. (
  • The field aims to find solutions for the repair of damaged tissues and enable the production of organs for transplantation. (
  • Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine surveys tools that are currently available to scientists and engineers in the field and how they have been used to fabricate physiologically appropriate tissues and organs. (
  • This book is therefore essential reading for all scientists, engineers, and physicians interested in the replacement, repair, or regeneration of human organs and tissues. (
  • MIT engineers report a new approach to creating three-dimensional samples of human tissue that could push researchers closer to their ultimate goal: tissues for therapeutic applications and replacement organs. (
  • Lead surgeon Dr. Paolo Macchiarini discusses the procedure and the benefits of tissue-engineered synthetic organs. (
  • The research program focuses on the design and synthesis of bioinspired materials that actively direct the fate of mammalian cells, and facilitate regeneration of damaged tissues and organs. (
  • They may to hold the key to restoring compromised immune systems and even regenerating tissues and organs damaged by disease or trauma. (
  • He proposed the joining of the terms tissue (in reference to the fundamental relationship between cells and organs) and engineering (in reference to the field of modification of said tissues). (
  • Myocardial tissue engineering (MTE), a strategy that uses materials or material/cell constructs to prolong patients' life after cardiac damage by supporting or restoring heart function, is continuously improving. (
  • Engineering sophisticated 3-D constructs mimicking tissues remains a technological challenge. (
  • The ability to develop tissue constructs with matrix composition and biomechanical properties that promote rapid tissue repair or regeneration remains an enduring challenge in musculoskeletal engineering. (
  • A prerequisite for successful tissue engineering is adequate vascularization that would allow tissue engineering constructs to survive and grow. (
  • To allow engineered tissue or organ constructs to survive and then thrive, a comprehensive network of healthy functional blood vessels is necessary for oxygen and nutrient delivery and waste product removal. (
  • Later tissue staining showed that the implants' vessels grew into the host tissue and the host's vessels grew into the constructs. (
  • However, in the case of the 3D-bioprinted NFC/A (60/40, dry weight % ratio) constructs, pluripotency was initially maintained, and after five weeks, hyaline-like cartilaginous tissue with collagen type II expression and lacking tumorigenic Oct4 expression was observed in 3D -bioprinted NFC/A (60/40, dry weight % relation) constructs. (
  • Chang, R. and Sun, W., Bi ofabrication of three-dimensional liver cell-embedded tissue constructs for in vitro drug metabolism models , LAP Lambert Academic Publishing, 2009, ISBN: 978-3-8383-2150-9. (
  • Histological and immunohistochemical analyses of the excised hybrid tooth-bone constructs revealed the presence of tooth tissues, including primary and reparative dentin and enamel in the tooth portion of hybrid tooth-bone implants, and osteocalcin and bone sialoprotein-positive bone in the bone portion of hybrid tooth-bone constructs. (
  • Cell-instructive and structural scaffolds, including biomimetic and nanotechnology-based scaffolds capable of delivering bioactive molecules at specific concentrations in a temporally and spatially defined fashion and to confer external geometry, internal architecture and mechanical properties to engineered constructs. (
  • Engineering of composite multi-tissue constructs, such as vascularized and innervated bone and skeletal muscle. (
  • NIDCR encourages research concerning functional and structural integration between the engineered tissue constructs and host DOC tissues. (
  • Optimization of grafting strategies of engineered tissue constructs. (
  • Vascularization and innervation of grafted engineered constructs. (
  • Small and large animal models to assess short- and long-term structural and functional integrity of engineered tissue constructs in vivo . (
  • This model is being tailored to develop a bridge to liver transplantation, a full replacement engineered liver and to model liver pathology such as liver fibrosis. (
  • The field of tissue engineering research offers hope to bridge the gap between organ shortage and transplantation needs. (
  • The grafts remained intact 6 months after transplantation, and boosted the regeneration of TMJ tissue more effectively than other graft designs that could only repair bone. (
  • Rising cost of organ transplantation, long waiting lines and rising medical applications of 3D printing in tissue and organ regeneration will be the major growth drivers for this market in the near future. (
  • Such use of the tissue will reduce the dependency on donors and reduce waiting time for transplantation. (
  • Clinical transplantation of a tissue-engineered airway. (
  • Among the major challenges now facing tissue engineering is the need for more complex functionality, biomechanical stability, and vascularization in laboratory-grown tissues destined for transplantation. (
  • With contributions from some of the leading experts in this field, this timely publication will prove essential reading for anyone with an interest in the field of tissue engineering. (
  • Based on previous reports and market trends, the field of tissue engineering is forecasted to continue to build revenue for the years to come. (
  • The burgeoning field of tissue engineering holds promise that replacement tissues can be constructed in the laboratory to recapitulate the functional requirements of native tissues. (
  • Finally it serves as a reference and teaching text for the rapidly increasing population of students and investigators in the field of tissue engineering. (
  • I have 30 years of experience, and specialize in the field of tissue engineering with specific knowledge in the areas of cartilage regeneration, adult stem cells and abdominal adhesion's. (
  • Biomarkers can be used as endpoints to monitor any genetic damage that could occur upon the preservation of cells and whether novel compounds designed to minimize damage from freezing/storing are effectively working at the molecular level.Recently, our laboratory has implemented the use of cellular biomarkers that can be used in the field of tissue engineering. (
  • Developments in the multidisciplinary field of tissue engineering have yielded a novel set of tissue replacement parts and implementation strategies. (
  • Neural tissue engineering is a specific sub-field of tissue engineering. (
  • From the control of cellular energetics to the processes of organ development and spinal cord regeneration these transformative discoveries are allowing new insights into the basic mechanisms of tissue growth, repair and regeneration in all metazoans and will permit novel approaches to the understanding, treatment and prevention of human disease. (
  • The Tissue Engineering & Organ Fabrication Lab uses cells combined with special plastics and natural materials, which act as the scaffolding upon which the living tissue is built. (
  • Organ engineering, as opposed to tissue engineering, poses significant challenges including the requirement for an immediately functional vascular network, functional parenchymal cells, and lymphatic and innervation potential. (
  • Creating an organ - The scaffold is then placed in an incubator that maintains optimal temperature, humidity, etc. for tissue growth and development. (
  • Creating an organ - This MRI image shows the lab-engineered vaginal organ inside the patient. (
  • Engineering an organ usually means starting with a scaffold to supply the basic structure. (
  • Tissue engineering and organ regeneration market encompasses those products used in medicine. (
  • The increasing use of tissue engineering and organ regeneration will reduce the use of metals and alloys in the body that are in most cases toxic and have a very high rejection. (
  • Do tissue engineered repairs and replacements need to exactly duplicate the structure and function of the normal tissue or organ? (
  • But the ability to generate organ tissue fragments that vascularize in the body-like pancreatic islets-had been an elusive goal until the current study, investigators said. (
  • Tissue and organ biofabrication or "printing" technologies that use layered manufacturing processes, such as rapid prototyping. (
  • The team hopes that the research will lead to improvements in replacing damaged tissue in the body, based on a better understanding of organ connectivity. (
  • Our research aims in this area are focused toward the creation of safe, appropriate and good quality in vitro models for the purpose of solving complex issues in craniofacial tissue disease. (
  • I'm an undergraduate physics major right now but I may go for biophysics because I am very interested in tissue engineering, specifically in-vitro meat production. (
  • What schools have graduate programs in the areas of muscle tissue or in-vitro meat development? (
  • Three-dimensional in vitro bioreactors, including nanotechnology and microfluidics-based bioreactors that recapitulate normal and pathological tissue development, structure and function. (
  • To assure that tissue-engineered materials are free of molecular changes that could occur during the in vitro development phase, cellular biomarkers are being identified that can be used during the manufacturing process. (
  • A few methods currently being investigated to treat CNS injuries are: implanting stem cells directly into the injury site, delivering morphogens to the injury site, or growing neural tissue in vitro with neural stem or progenitor cells in a 3D scaffold. (
  • Tissue engineering , scientific field concerned with the development of biological substitutes capable of replacing diseased or damaged tissue in humans. (
  • By the early 1990s the concept of applying engineering to the repair of biological tissue resulted in the rapid growth of tissue engineering as an interdisciplinary field with the potential to revolutionize important areas of medicine . (
  • Tissue engineering integrates biological components, such as cells and growth factors, with engineering principles and synthetic materials. (
  • Published 24 times per year, the flagship provides a fundamental understanding of structure-function relationships in normal and pathologic tissues with the ultimate goal of developing biological substitutes. (
  • Dr. Voronov begins as an Assistant Professor at the New Jersey Institute of Technology's York Otto Department of Chemical, Pharmaceutical and Biological Engineering. (
  • When she decided she wanted to return to school, it only took a meeting with Schiele for her to decide on biological engineering and pursing a doctorate. (
  • Now, in a feat of reverse tissue engineering, Stanford University researchers have begun to unravel the complex genetic coding that allows embryonic cells to proliferate and transform into all of the specialized cells that perform myriad biological tasks. (
  • A large proportion of our work focuses on materials that can stimulate beneficial biological responses from the body, such as the stimulation of tissue repair. (
  • Tissue engineering, referring to the technologies that use physical, chemical, biological and engineering processes to control and direct the aggregate behaviour of cells, has become a field of increasing commercial interest. (
  • The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of reproducible methods. (
  • Functional Requirements of Engineered Tissues * Functional Requirements for the Engineering of a Blood Vessel Substitute * In vivo Force and Strain of Tendon, Ligament and Capsule * Requirements for Biological Replacement of the Articular Cartilage at the Knee Joint * Functional Requirements: Cartilage * Part III. (
  • The goal of tissue engineering is to improve upon or completely replace biological functions, and this is primarily carried out through a combination of engineering materials, cells, and biochemical factors. (
  • I have published on such topics as nanosilicon tissue engineering, luminescent semiconductor nanostructures and semiconductors fabricated on biological templates. (
  • Yildirim E.D., Gandhi M., Fridman A., Guceri S. and Sun W., Plasma Surface Modification of Three Dimensional Poly ( e -Caprolactone) Scaffolds for Tissue Engineering Application , in Plasma Assisted Decontamination of Biological and Chemical Agents , NATO Science for Peace and Security Series A: Chemistry and Biology, Springer Publishing, 2008: ISBN: 1874-6468, 191-202. (
  • Building on this rich history, the 2021 GRC and GRS on Biomaterials and Tissue Engineering will focus on the design, function, and translation of bio-instructive materials, with a particular emphasis on material modulation of biological activity. (
  • For example, conjugation of biomolecules and drugs to biomaterials can augment or suppress their native biological activities, in part by changing the way in which they are presented to cells and tissues. (
  • The procedure most often used in cartilage tissue engineering involves a suitable combination of seeded cells, a biocompatible scaffold, and biological factors that support cartilage formation. (
  • In this work, we attempted to design a suitable combination of cells and biological factors and a suitable scaffold for cartilage tissue engineering. (
  • Major discoveries from his laboratory have centered on the control of cell fate and tissue formation in contract with materials that are tunable in both their biological content and mechanical properties. (
  • Tissue engineering is an emerging new area of biotechnology that will provide replacement tissues for patients, as well as complex, functional biological systems for research and testing in the pharmaceutical industry. (
  • Fundamentals to many tissue-engineered devices are problems of inflammation associated with how biological cells respond to a given device when inserted into the body. (
  • Tissue engineering is a biomedical engineering discipline that uses a combination of cells, engineering, materials methods, and suitable biochemical and physicochemical factors to restore, maintain, improve, or replace different types of biological tissues. (
  • Engineered replacements for musculoskeletal tissues generally require extensive ex vivo manipulation of stem cells to achieve controlled differentiation and phenotypic stability. (
  • The ability to control cell differentiation using cell-instructive scaffolds that have biomechanical properties approximating those of native tissue would represent a transformative advance in functional tissue engineering. (
  • The goal of this study was to develop a bioactive scaffold capable of mediating cell differentiation and formation of an extracellular matrix with the biochemical composition and mechanical features that mimic native tissue properties. (
  • Current approaches require extensive cell manipulation ex vivo, using exogenous growth factors to drive tissue-specific differentiation, matrix accumulation, and mechanical properties, thus limiting their potential clinical utility. (
  • The ability to induce and maintain differentiation of stem cells in situ could bypass these steps and enhance the success of engineering approaches for tissue regeneration. (
  • In 2009, Zhang and colleagues demonstrated the dramatic effect of tissue-specific ECM compounds on the enhancement of cell proliferation and differentiation from each specific tissue 10 . (
  • We are a 'biomaterials' and 'stem-cell based tissue engineering' laboratory and use biomaterials to culture, proliferate and induce differentiation in stem cells to understand how the ECM-stem cell interaction modulates cell fate and turnover. (
  • This is the first report, to our knowledge, of the preparation of an injectable in situ-forming click-crosslinked hyaluronic acid (Cx-HA) hydrogel (Cx-HA-CM) containing chemical immobilized cytomodulin-2 (CM), a chondrogenic differentiation factor, and on the utility of human periodontal ligament stem cells (hPLSCs) as a cell source for cartilage tissue engineering. (
  • Cell differentiation during sphere formation and the integrity of the osteomicrospheres was evaluated by analyzing the immunohistochemical expression of osteonectin, osteocalcin, and collagen type I. Transmission electron microscopy facilitated the proof of the tissue-like microstructure inside the osteomicrospheres and the. (
  • The differentiation of embryonic stem cells (ESC) into tissue-specific cells utilizes either monolayer cultures or three-dimensional cell aggregates called embryoid bodies (EB). (
  • Isolation, characterization, expansion, and differentiation of stem and progenitor cells for engineering of dental, oral and craniofacial (DOC) tissues. (
  • Scientific advances in biomaterials, stem cells, growth and differentiation factors, and biomimetic environments have created unique opportunities to fabricate or improve existing tissues in the laboratory from combinations of engineered extracellular matrices ("scaffolds"), cells, and biologically active molecules. (
  • Micropatterning Techniques to Control Cell-Biomaterial Interface for Cardiac Tissue Engineering. (
  • Many hydrogels, including natural polymer hydrogels, synthetic polymer hydrogels, and natural/synthetic hybrid hydrogels are employed for cardiac tissue engineering. (
  • In this review, types of hydrogels used for cardiac tissue engineering are briefly introduced. (
  • Li Z, Guan J. Hydrogels for Cardiac Tissue Engineering. (
  • This method opens new avenues in the development of bioactive implants that circumvent the need for ex vivo tissue generation by enabling the long-term goal of in situ tissue engineering. (
  • Diffusion and in vivo capillary networks can only support tissue less than 2 mm thick, preventing practical application [ 1 ]. (
  • What are the mechanical properties of these tissues when subjected to expected in vivo stresses and strains, as well as under failure conditions? (
  • These in vivo data provide mechanical thresholds that tissue repairs/replacements will likely encounter after surgery. (
  • One of our goals is to define pharmacology for enhancing maintenance and repair of adult tissues in vivo. (
  • The reconstruction of skeletal muscle tissue either lost by traumatic injury, tumor ablation, or due to congenital abnormalities is hampered by the lack of availability of functional substitutes to this native tissue. (
  • The book helps readers to gain a working knowledge about the nanotechnology aspects of tissue engineering and will be of great use to those involved in building specific tissue substitutes in reaching their objective in a more efficient way. (
  • Cell- and tissue-based therapies are gaining ground, but basic principles still need to be addressed to ensure successful development of clinical treatments. (
  • Presents procedures and protocols of research methods that will be adopted by the tissue engineering community as research is translated into clinical applications to help the field to grow and mature. (
  • Efforts to develop a tissue engineered lung have only recently begun to gain momentum despite a significant clinical need for patients of all ages. (
  • Therefore, overcoming the challenges of neovascularization is critical in the clinical applicability of tissue engineering. (
  • The vision of Brown and other leaders to make BU into a highly collaborative and top-tier research institution reached a milestone with the opening of the ERC, which has the goal to develop personalized heart tissue for clinical use. (
  • During the ceremony, Dean Kenneth R. Lutchen remarked that it wasn't just the idea to create functionalized heart tissue that was the center of the grant, but "it was the idea that we could use these breakthrough technologies to do this at a scale in a low-cost and easy-to-manufacture way that could have a massive impact on cardiovascular medicine and transform clinical care. (
  • The article is misleading as tissue engineering research is in its infancy and products of sufficient quality for routine clinical use remain a long way away. (
  • Additionally, a summary of ongoing clinical trials of tissue engineered products is provided, organized by trial phase, tissue type, and sponsor. (
  • This market is broken down into key applications of therapies (clinical use) and tissue models (research use). (
  • Functional Tissue Engineering will be useful to students and researchers as it will remind tissue engineers of the clinical importance of restoring function to damaged tissue and structures. (
  • And although stem cell-based tissue engineering has tremendous therapeutic potential, its future clinical use still faces the critical challenge of ensuring a blood supply to nourish the transplanted tissues, according to researchers. (
  • These results demonstrate the utility of a hybrid tooth-bone tissue-engineering approach for the eventual clinical treatment of tooth loss accompanied by alveolar bone resorption. (
  • Biocompatibility, immunogenicity, biotoxicity, and biodegradability of tissue engineering biomaterials and scaffolds in animal models, including pre-clinical large animal models. (
  • The clinical development underlying such scientific research includes a number of novel tissue-engineered products. (
  • This report seeks to provide an update of the tissue engineering industry from 2011 to 2018. (
  • Adapted and abridged from " An Overview of the Tissue Engineering Market in the United States from 2011 to 2018 ," Tissue Engineering , Part A , September 4, 2018, published by Mary Ann Liebert, Inc. (
  • A market forecast for tissue engineered products is provided for the years 2018 - 2028, where the total value for tissue engineered products is predicted to surpass $4.8 billion. (
  • Separately, a forecast for 3D bioprinters, an up-and-coming technology for tissue engineering, is also provided for the years 2018 - 2028. (
  • The global market for tissue engineering and regeneration should grow from $24.7 billion in 2018 to $109.9 billion by 2023 with a compound annual growth rate (CAGR) of 34.8% from 2018 to 2023. (
  • Hydrogels, because of their tissue-like properties, have been used as supporting matrices to deliver cells into infarcted cardiac muscle. (
  • Bioactive and biocompatible hydrogels mimicking biochemical and biomechanical microenvironments in native tissue are needed for successful cardiac tissue regeneration. (
  • To help address that shortage, researchers at MIT, Rockefeller University, and Boston University have developed a new way to engineer liver tissue, by organizing tiny subunits that contain three types of cells embedded into a biodegradable tissue scaffold. (
  • In a study of mice with damaged livers, the researchers found that after being implanted in the abdomen, the tiny structures expanded 50-fold and were able to perform normal liver tissue functions. (
  • To boost their hepatocyte population, the researchers decided to take advantage of a key trait of liver cells, which is that they can multiply to generate new liver tissue. (
  • McGowan Institute researchers-led by Stephen Badylak, DVM, PhD, MD -are working on a method that uses natural scaffolds seeded with the patient's own cells to encourage the growth of healthy tissue instead of scar tissue. (
  • This book was written to serve as a reference for researchers seeking to learn about the TMJ, for undergraduate and graduate level courses, and as a compendium of TMJ tissue engineering design criteria. (
  • The researchers used the reverse-engineering technique to study the cells in the alveoli, the small, balloon-like structures at the tips of the airways in the lungs. (
  • Now, researchers in Institute Professor Robert Langer's lab at MIT have used a novel cocktail of cells to coax muscle tissue to develop its own vascular network, a process called pre-vascularization. (
  • Using two non-invasive live imaging techniques (labeled lectin injected into the tail vein and a luminescent luciferase-based system), the researchers could watch the host's blood flow into the engineered vessels. (
  • Toronto researchers have invented a new device that may allow for the uniform, large-scale engineering of tissue. (
  • Researchers within the Matrix Biology & Tissue Repair Research Unit have developed a human 3D cartilage model engineered from stem cells. (
  • Researchers tissue-engineered human pancreatic islets in a laboratory that develop a circulatory system, secrete hormones like insulin and successfully treat sudden-onset type 1 diabetes in transplanted mice. (
  • After the tissue-engineered islets were transplanted into humanized mouse models of severe type 1 diabetes, they resolved the animals' disease, report researchers. (
  • Researchers though face a range of problems in generating tissue which can be circumvented by employing nanotechnology. (
  • By treating the scaffold/stem cell structure with chemical cues, or growth factors, known to stimulate the formation of specific cell types, the researchers coaxed the stem cells to form tissues with characteristics of developing human cartilage, liver, nerves and blood vessels. (
  • The scaffold provides physical cues for cell orientation and spreading, and pores provide space for remodeling of tissue structures," the researchers wrote. (
  • The researchers who developed and conducted studies on the tissue published their work in the Nature Medicine . (
  • Researchers have earlier developed intestinal tissue in the laboratory to study various intestinal functions using cells called pluripotent stem cells. (
  • The researchers used the tissue to study a condition called Hirschsprung Disease or toxic megacolon . (
  • In fact, the researchers were able to transplant the tissue into laboratory mice and found that it functions just like the normal intestine. (
  • The researchers also developed a control scheme to simulate compliant tissues using the shape display and an actuated positioner. (
  • The researchers eventually hope to build a cubic millimetre of tissue which would comprise of around 100,000 cells and 900,000 million connections. (
  • Current approaches for replacing the damaged bone tissues include the use of bone grafts (ie, auto-grafts or allografts). (
  • We also provide our recent work on tissue engineering for DSAEK grafts using cultured HCECs. (
  • Scientists have engineered tissue grafts that, in pigs, regenerated both bone and cartilage in the temporomandibular joint (TMJ), a part of the jaw that can cause debilitating pain and disability when damaged. (
  • David Chen and colleagues took a different approach where they engineered tissue grafts that, unlike previous bone-focused designs, can repair both cartilage and bone within the ramus-condyle unit of the TMJ. (
  • Chen, D., (2020) Tissue engineered autologous cartilage-bone grafts for temporomandibular joint regeneration. (
  • Imagine a machine that makes layered, substantial patches of engineered tissue-tissue that could be used as grafts for burn victims or vascular patches. (
  • We have worked closely with scientists and engineers at MIT and have studied 27 tissues of the body. (
  • In its early days, tissue engineering was driven by material scientists who designed novel bio-resorbable scaffolds on which to seed cells and grow tissues. (
  • Scientists genuinely believe that in years to come, labs will be filled with rows of hearts and livers that can be taken off the shelf and tailored to you," says Lindsey Dew, who has just started a PhD in tissue engineering at the University of Sheffield . (
  • The placement of the cells is so precise, in fact, that scientists can spell words (such as "Toronto," shown here) and can precisely mimic the natural placement of cells in living tissues. (
  • Scientists are taking a close look at fish skeletons for inspiration to solve engineering problems. (
  • The 2015 Gordon Research Conference on Biomaterials and Tissue Engineering will bring together world-class clinicians, scientists and engineers to discuss materials-related strategies for disease remediation and tissue repair. (
  • Substitute tissues of the renal system, including urinary bladders and urethras , have also been engineered and transplanted successfully, thereby broadening therapeutic opportunities for complicated renal disorders. (
  • Organovo's NovoTissue ® , a 3D-bioprinted liver therapeutic tissue used for the treatment of alpha-1 antitrypsin deficiency (A1AT) was granted orphan drug designation by the FDA in December 2017. (
  • Globally recognized as the trusted source for critical, even controversial coverage of emerging hypotheses and novel findings on stem cells of all tissue types and their potential therapeutic applications. (
  • Hence, alternative therapeutic approaches including tissue engineering using stem/progenitor cells and/or their derived progeny to rescue tissue function are urgently needed. (
  • Controlled release of therapeutic factors in turn will enhance the efficacy of tissue engineering. (
  • Intestinal tissue with nerve supply produced in the laboratory has several research and therapeutic implications in medicine. (
  • Therapeutic products are further segmented into cell therapy, gene therapy, tissue engineering, and small molecules and biologics based on product type. (
  • Therapeutic products are also categorized into synthetic materials, biologically derived materials, genetically engineered materials, and small molecules and biologics based on material. (
  • The Cartilage Tissue Engineering Laboratory in the Department of Bioengineering at the University of California, San Diego was formed in July, 1992 by Prof. Robert Sah . (
  • First, we chose human periodontal ligament stem cells (hPLSCs) as a cell source for the cartilage tissue engineering in this work because hPLSCs can easily be harvested in large quantities from teeth obtained during dental repair or surgical procedures. (
  • Tissue specific vascular networks have been developed and are being optimized for use in engineered lung and liver tissues. (
  • This liver is comprised of an engineered biomimetic vascular network and parenchymal chamber separated by a semi-permeable membrane and utilizes our engineered vascular network technology. (
  • Common applications of tissue engineering involve ischemic tissue (such as in myocardial infarction and peripheral vascular disease), or trauma resulting in loss of vascularization. (
  • This tri-culture system shows a whole new way of creating a vascular network in the tissue," summarizes Langer. (
  • Pancreatic islets tissue-engineered in the current generated by the process not only quickly developed a vascular network after transplant into animal models of type 1 diabetes, the tissues also functioned efficiently as part of the endocrine system-secreting hormones like insulin and stabilizing glycemic control in the animals. (
  • In the book also explained are fabrication techniques for production of scaffolds to a series of tissue-specific applications of scaffolds in tissue engineering for specific biomaterials and several types of tissue (such as skin bone, cartilage, vascular, cardiac, bladder and brain tissue). (
  • Common MTE strategies include an engineered 'vehicle', which may be a porous scaffold or a dense substrate or patch, made of either natural or synthetic polymeric materials. (
  • One aspect of Jacot's research involves using stem cells from an infant's own amniotic fluid to generate natural heart tissue that he hopes will vastly improve upon the synthetic patches currently used in heart repair. (
  • Scaffold materials used to engineer tissues have traditionally been synthetic polymers, biocompatible porous inorganic materials and purified extracts of natural extra-cellular matrices (ECMs). (
  • However, a lack of a formal regulatory framework has the potential for creating differences in the marketplace for products where there is no scientific basis, thereby leading to inappropriate competitive disadvantages for some products.A new research area of tissue engineering involves the investigation of how living cells interact and respond to synthetic biomaterial surfaces. (
  • Examples include replacement skin as a synthetic dermal matrix for burns patients/chronic ulcer patients, nerve guidance channels to enhance repair of damaged peripheral nervous tissue or suitable material for the design of 'second generation' coronary artery stents for patients with heart disease. (
  • The term first appeared in a 1984 publication that described the organization of an endothelium-like membrane on the surface of a long-implanted, synthetic ophthalmic prosthesis The first modern use of the term as recognized today was in 1985 by the researcher, physiologist and bioengineer Y.C Fung of the Engineering Research Center. (
  • Inadequate vascularization remains one of the major challenges facing tissue engineering. (
  • An accompanying News and Views commentary says this "landmark paper" provides "a compelling demonstration of the benefits of pre-vascularization for engineering larger pieces of tissue. (
  • Unfortunately, the engraftment success rate is relatively low because the tissues lose their vascularization and blood supply as islets are being processed before transplant. (
  • We demonstrate in this study that the self-condensation cell culturing system promotes tissue vascularization. (
  • This part of the program welcomes basic and translational research directed at patterning of host tissue microenvironment to resolve acute and chronic inflammation, to reduce tissue fibrosis, promote vascularization, innervation and scarless wound healing. (
  • This book outlines the biomechanical, biochemical, and anatomical characteristics of the disc and condylar cartilage, and also provides a historical perspective of past and current TMJ treatments and previous tissue engineering efforts. (
  • Despite its early success, tissue engineers have faced challenges in repairing or replacing tissues that serve a predominantly biomechanical function. (
  • These 21 companies made an estimated $9 billion in sales of tissue engineering-related products in 2017. (
  • Leadership from BU and the College of Engineering cut the ribbon celebrating the opening of the Engineering Research Center on October 2, 2017. (
  • DMM comprising muscle and fascia ECM as a whole unit can thus provide not only a clinically translatable platform for musculofascial tissue repair and regeneration but also a useful standard for scaffold design in musculofascial tissue engineering. (
  • Before that, he worked in the engine room of the Waldorf Astoria Hotel in New York, where he constructed a small laboratory at the hotel for famous electrical engineer Nikola Tesla. (
  • My laboratory is currently pursuing different applications of the technology-different tissues," says Guenther. (
  • The MPB system has significant long-term commercial potential both in the laboratory and the clinic as a tool to facilitate the translation of stem cell developments into practical replacement tissues for neural, hematopoietic, and mesenchymal lineages. (
  • Takebe's and Taniguchi's research team already demonstrated the ability to use a "self-condensation" cell culture process using iPS cells to tissue engineer three-dimensional human liver organoids that can vascularize after transplant into laboratory mice. (
  • Professor Yves Grohens is the Director of the LIMATB (Material Engineering) Laboratory of Universit de Bretagne Sud, France. (
  • In many cases, such as in implants and engineered tissues, the interaction of the cells with the biomaterial is one of the main determinants of the success of the system. (
  • When implanted in living mice and rats, these tissues integrated more robustly with the body's own tissues than similar implants without blood vessels. (
  • We anticipate that stringent testing of these products will be carried out before they are considered for human implantation.Furthermore we disagree with the statement 'Human tissue engineered products may carry greater risks' as current momentum has shifted towards developing autologous products, devoid of the complication and drawbacks associated with artificial implants. (
  • And finally, can tissue engineers mechanically stimulate these implants before surgery to produce a better repair outcome? (
  • Developmental Biology and Musculoskeletal Tissue Engineering: Principles and Applications focuses on the regeneration of orthopedic tissue, drawing upon expertise from developmental biologists specializing in orthopedic tissues and tissue engineers who have used and applied developmental biology approaches. (
  • Recent developments in orthopedic tissue engineering have sought to recapitulate developmental processes for tissue repair and regeneration, and such developmental-biology based approaches are also likely to be extremely amenable for use with more primitive stem cells. (
  • The National Science Foundation- (NSF) funded scientist is designing new tissue engineering approaches with the aim of making pediatric heart surgery less invasive, possibly even unnecessary. (
  • Whereas traditional tissue-engineering scaffolds were based on hydrolytically degradable macroporous materials, current approaches emphasize the control over cell behaviors and tissue formation by nano-scale topography that closely mimics the natural extracellular matrix (ECM). (
  • A wide range of approaches are still being explored in tissue engineering, and this report covers both the most innovative and cutting-edge techniques as well as more established technologies. (
  • However, detailed characterization of musculofascial tissues' ECM (particularly, of fascia) from large animals is still lacking. (
  • Characterization of in situ stem and progenitor populations and stem cell niches that contribute to tissue regeneration of DOC tissues. (
  • Tissue Engineering brings together scientific and medical experts in the fields of biomedical engineering, material science, molecular and cellular biology, and genetic engineering. (
  • Working with Christopher Chen, a professor of biomedical engineering at Boston University, Bhatia's team designed microfabricated structures that incorporate spherical "organoids" made of hepatocytes and fibroblasts, as well as cords of endothelial cells, which are the building blocks of blood vessels. (
  • International Journal for Numerical Methods in Biomedical Engineering. (
  • The book provides a solid starting point for elucidating and exploiting the different aspects of cellular interactions with materials for biomedical engineering. (
  • When I came to work with Bob Langer for my postdoc, it was my dream to vascularize a tissue," recalls first author Shulamit Levenberg, who is now on the faculty of the biomedical engineering department at Technion in Haifa, Israel where she completed these studies. (
  • I have 20 years of experience in the field of biomedical engineering, with specific knowledge of biomaterial, growth factor, and animal models. (
  • I have 10 years of experience, and specialize in the field of biomedical engineering with specific knowledge in the areas of tissue engineering, biotechnologies and micro fluidics. (
  • I have 16 years of experience and specialize in the field of biomedical engineering, with specific knowledge of medical device design, biomaterials and tissue engineering. (
  • The artificial 3D-printed tissue will be used as a drug testing platform, and the company plans to file an IND with the FDA by 2020. (
  • The artificial patches must be replaced as a child grows, whereas, if successful, the bioengineered tissue 'will grow along with the patient and be functional tissue, not just a piece of plastic in the heart,' Jacot says. (
  • Tissue engineers use many methods, in​cluding the manipulation of artificial and natural materials that provide structure and biochemical instructions to young cells as they grow into specific kinds of tissue. (
  • Biomaterials with nano-scale organizations have been used as controlled release reservoirs for drug delivery and artificial matrices for tissue engineering. (
  • Unique to this new approach to tissue engineering, however, and unlike more typical methods for tissue engineering (for instance, scaffolding, the seeding of cells onto an artificial structure capable of supporting three-dimensional tissue formation) cells planted onto the mosaic hydrogel sheets are precisely incorporated into the mosaic hydrogel sheet just at the time it's being created-generating the perfect conditions for cells to grow. (
  • Our strategic focus areas are 1) Cardiac, 2) Neural, 3) Bone tissue and 4) Gastro-intestinal tissue engineering. (
  • I have published on such topics as cell delivery to the central nervous system, localized delivery to the spinal cord and neural tissue engineering. (
  • Neural tissue engineering is primarily a search for strategies to eliminate inflammation and fibrosis upon implantation of foreign substances. (
  • The need for neural tissue engineering arises from the difficulty of the nerve cells and neural tissues to regenerate on their own after neural damage has occurred. (
  • Recent research into creating miniature cortexes, known as corticopoiesis, and brain models, known as cerebral organoids, are techniques that could further the field of neural tissue regeneration. (
  • The native cortical progenitors in corticopoiesis are neural tissues that could be effectively embedded into the brain. (
  • Cerebral organoids are 3D human pluripotent stem cells developed into sections of the brain cortex, showing that there is a potential to isolate and develop certain neural tissues using neural progenitors. (
  • Research into the stimulation of PNS neurons in patients with paralysis and prosthetics could further the knowledge of reinnervation of neural tissue in both the PNS and the CNS. (
  • This research is capable of making one difficult aspect of neural tissue engineering, functional innervation of neural tissue, more manageable. (
  • Other concerns of neural tissue engineering include establishing safe sources of stem cells and getting reproducible results from treatment to treatment. (
  • Molecules that promote the regeneration of neural tissue, including pharmaceutical drugs, growth factors known as morphogens, and miRNA can also be directly introduced to the injury site of the damaged CNS tissue. (
  • Abstract This project will develop and demonstrate a prototype Modular Perfusion Bioreactor (MPB) for tissue engineering applications. (
  • In general, the main issues currently under investigation include the sourcing of an appropriate cell source, design of biomaterials for guided tissue growth, provision of a biomolecular stimulus to enhance cellular functions and the development of bioreactors to allow for prolonged periods of cell culture under specific physiological conditions. (
  • While many unique styles of bioreactors have been proposed for various types of stem cell and tissue cultures, there is not a single, easy-to-use device that accommodates the multiple diverse needs of multiple tissue culture types. (
  • Research in the Bell Center is intended to elucidate the molecular, genetic and cellular mechanisms underlying the growth and replacement of highly differentiated tissues during development, physiological turnover and repair following injury. (
  • Musculoskeletal tissues have an inherently poor repair capacity, and thus biologically-based treatments that can recapitulate the native tissue properties are desirable. (
  • Smi's 2nd Annual Tissue Engineering event will highlight the current key issues in the industry and the opportunities for the future, focusing on tissue repair, replacement and regeneration. (
  • Unfortunately, these tissues have limited ability to heal, necessitating the development of treatments for repair or replacement. (
  • Tissue engineering has focused primarily on repair: burned skin, muscle shorn off during in an accident, a dysfunctional bladder. (
  • What's even more exciting than being able to make skeletal muscles for reconstructive surgery or to repair congenitally defective muscles, for instance, is that this a generic approach that can be applied towards making other complex tissues. (
  • More information on the Matrix Biology & Tissue Repair Research Unit . (
  • MicroRNAs (miRNAs) influence several processes in damage, repair, and regeneration of tissues. (
  • Two areas in which the department has established special leadership are cellular mechanobiology, which focuses on understanding the interaction and conversion between force-based and biochemical information in living systems, and stem cell engineering, which includes platforms to expand, implant, and mobilize stem cells for tissue repair and replacement. (
  • Play media While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc. (
  • In this paper, the authors present principles of functional tissue engineering that should be addressed when engineering repairs and replacements for load-bearing structures. (
  • Incorporating each of these principles of functional tissue engineering should result in safer and more efficacious repairs and replacements for the surgeon and patient. (
  • Professor Sabu Thomas is Professor of Polymer Science & Engineering, School of Chemical Sciences and the Director of Centre for Nanoscience and Nanotechnology at Mahatma Gandhi University, India. (
  • She received Masters in Engineering in 'Nanotechnology in Medical Science' from Amrita Centre for Nanosciences, Kochi, India. (
  • NIDCR encourages research that takes advantage of advances in biology, chemistry, material science, nanotechnology, computer science, and engineering to facilitate regeneration of endogenous DOC tissues. (
  • Substitute tissues can be produced by first seeding human cells onto scaffolds, which may be made from collagen or from a biodegradable polymer . (
  • In this study, honeycomb collagen sheet was used for three-dimensional (3D) cultures of human skin fibroblasts and characterized as an effective and suitable scaffold for dermal tissue engineering. (
  • Our study proved that honeycomb collagen sheet is a mechanically stable, biocompatible and biodegradable scaffold for dermal tissue engineering, and also potentially useful for other cell-based therapies and tissue engineering applications. (
  • Collagen type III-positive connective tissue resembling periodontal ligament and tooth root structures were present at the interface of bioengineered tooth and bone tissues. (
  • Collagen Type I is the largest component of the bone tissue. (
  • The platelet-like bioapatite crystals are inserted in a parallel fashion into the collagen fibrils, replacing the water found in other tissue collagens ( Figure 1 ). (
  • In this hydrogel, many cell types are shown to adhere and proliferate, and injection of the gel with or without cells, promotes regeneration of tissues such as bone, brain and cardiac tissue. (
  • The biomaterials are then mixed, causing a chemical reaction that forms a "mosaic hydrogel"-a sheet-like substance compatible with the growth of cells into living tissues, into which different types of cells can be seeded in very precise and controlled placements. (
  • These findings not only provide more supramolecular hydrogel candidates for tissue engineering, but also offer a new strategy for designing biomaterials that mimic nature. (
  • Cardiomyocytes and stem cells are utilized to regenerate cardiac tissue. (
  • It provides information on methodologies for designing and using biomaterials to regenerate tissue, on novel nano-textured surface features of materials (nano-structured polymers and metals e.g.) as well as on theranostics, immunology and nano-toxicology aspects. (
  • These therapies are extensively used to replace and regenerate the cells, genes and tissues in a patient's body. (
  • Furthermore, these matrices can serve as a starting material for the development of tissue engineering products. (
  • From a materials point of view, both the drug-delivery vehicles and tissue-engineering scaffolds need to be biocompatible and biodegradable. (
  • Tissue engineering requires a mechanically stable, biocompatible, and biodegradable scaffold that permits cell adherence and proliferation, allows preservation of cell-specific properties, and suitable for surgical implantations. (
  • Pluripotent embryonic stem cells are immature cells that have the capability to mature into a wide range of blood and tissue cells. (
  • Tissue engineering merges aspects of engineering and biology, and many rapid achievements in this field have arisen in part from significant advances in cell and molecular biology. (
  • Inflammation resolution, wound healing, connective tissue remodeling and scarless wound healing. (
  • Materials-based strategies to alter bioavailability, such as immobilization, controlled release, or gradient generation, along with the precise method of bioconjugation, have also been shown to actively influence how instructive cues are presented and thereby how cells and tissues respond. (
  • To do so, naturally-derived and tissue-specific ECM can serve as a basis to develop tissue engineering products for SUI therapies. (
  • We have investigated the utility of a tissue-engineering approach to provide corrective therapies for tooth-bone loss. (
  • The advancements in scaffold-supported cell therapy for musculoskeletal tissue engineering have been truly dramatic in the last couple of decades. (
  • The PhD student will employ human stem cells and iPS cell technology, along with other molecular biology and cell biology techniques as well as microfluidic technology in musculoskeletal tissue engineering. (
  • The preeminent, biomedical journal bringing together the principles of engineering and life sciences to advance the field with cutting-edge research and applications on all aspects of tissue growth and regeneration. (
  • We established the decellularization of porcine urethras to produce acellular urethra bioscaffolds for future tissue engineering applications, using bioscaffolds or bioscaffold-derived soluble products. (
  • Nanostructured materials for applications in drug delivery and tissue engineering. (
  • This review summarizes the most recent development in utilizing nanostructured materials for applications in drug delivery and tissue engineering. (
  • The biggest applications for engineered tissues are in research and development, and in medicine. (
  • Engineered cell growth has been present over the past many years with little or no applications in medicine. (
  • Several cardiovascular applications such as heart valve and myocardial tissue regeneration is also expected to provide excellent sustainability for the growth of the market. (
  • Tissue Chips) for drug screening, studying mechanisms of disease and other applications. (
  • Tissue engineering often involves the use of cells placed on tissue scaffolds in the formation of new viable tissue for a medical purpose but is not limited to applications involving cells and tissue scaffolds. (
  • The continued success of tissue engineering and the eventual development of true human replacement parts will grow from the convergence of engineering and basic research advances in tissue, matrix, growth factor, stem cell, and developmental biology, as well as materials science and bioinformatics. (
  • MACI is the first FDA-approved tissue engineering product that combines scaffolds and autologous cells from the patient. (
  • Keegan, 1875-1942, supervised the installation and testing of all mechanical and electrical equipment in the Vanderbilt Hotel in New York while it was under construction and was chief engineer from its 1910 opening to his death in 1942. (
  • Mechanical Engineering Chair Professor Alice White (ME, MSE) will lead nanomechanics research, and Photonics Center Director Professor Thomas Bifano (ME, MSE) will lead imaging research. (
  • The resulting tissues, cites Lian Leng, lead author on the project and a 3rd year PhD Candidate in the Department of Mechanical and Industrial Engineering, are remarkably stable. (
  • Second, the mechanical properties of the native tissues must be established for subfailure and failure conditions. (
  • This subset is important, given that the mechanical properties of the designs are not expected to completely duplicate the properties of the native tissues. (
  • Application of mechanical forces and electrical stimulation in shaping functional characteristics of engineered tissues. (
  • Often, the tissues involved require certain mechanical and structural properties for proper functioning. (
  • The invention also relates to a monolithic implant as thereby manufactured, and the use of such an implant as a scaffold for tissue-engineering and as a transplant tissue in reconstructive or replacement surgery. (
  • CAMBRIDGE, Mass. - For years, a major obstacle has dashed the hopes of creating "replacement parts" for the human body: the lack of an internal, nourishing blood system in engineered tissues. (
  • What is the best source of cells for tissue engineering of both bone and cartilage? (
  • This session will have presentations on the engineering of skeletal tissue, with topics such as the design of materials that promote osteroblast growth or mineralization. (
  • These materials are called scaffolds because they provide support and materials for tissue regrowth in the same way that a scaffold supports workers and materials for a building under construction. (
  • Once you hear that it's possible, you have to do it," said Professor David Bishop (ECE, Physics), head of the Division of Materials Science & Engineering and director of the ERC. (
  • Tissue engineering has the potential to achieve this by combining materials design and engineering with cell therapy. (
  • There's a lot of interest in soft materials, particularly biomaterials," explains Guenther of the materials that help create functional tissue cultures, "but until now no one has demonstrated a simple and scalable one-step process to go from microns to centimeters. (
  • Tissue engineering merges the disciplines of study like cell biology, materials science, engineering and surgery to enable growth of new living tissues on scaffolding constructed from implanted polymeric materials. (
  • Dr.Neethu Ninan was awarded PhD in Materials Engineering from Universite de Bretagne Sud, Lorient, France. (
  • Valuable information is available on a broad range of technologies including material separation, laser processes, measuring techniques and robot engineering in addition to testing methods and coating and materials analysis processes. (
  • Professor Desai's research spans multiple disciplines including materials engineering, cell biology, tissue engineering, and drug delivery. (
  • Research in the Healy Lab emphasizes the relationship between materials and the cells or tissues they contact. (
  • The present review will discuss how investigators have approached the challenge of neovascularization in tissue engineering through protein therapy and angiogenic growth factors, as well as cell therapy through endothelial cells and endothelial progenitor cells. (
  • Optimization, standardization and side-by-side comparison and quality control of stem and progenitor cell sources for use in DOC tissue engineering and regeneration. (
  • Within 90 days, the scaffold was replaced with functional tissue. (
  • Functional Tissue Engineering addresses the key issues in repairing and replacing load-bearing structures effectively. (
  • Functional Tissue Engineering also provides an invaluable resource to help tissue engineers incorporate these functional criteria into the design, manufacture, and optimization of tissue engineered products. (
  • Ligament Healing: Present Status and the Future of Functional Tissue Engineering * Native Properties of Cardiovascular Tissues: Guidelines for Functional Tissue Engineering * Functional Properties of Native Articular Cartilage * Excitability and Contractility of Skeletal Muscle: Measurements and Interpretations * Part II. (
  • An evolving discipline called "functional tissue engineering" (FTE) seeks to address these challenges. (
  • Services include tools, along with cell and gene bank charges, where the cells and tissues are stored and treated properly pre- and post-experimentation and treatment. (
  • The invention provides a process for the production of a monolithic implant for use in tissue engineering, the process comprising the steps of plasticising at least one biocompatible material into a paste, extruding the paste through a die, and drying the extruded material to eliminate an aqueous and/or. (
  • Evaluation of cellular components' removal from musculofascial tissues. (
  • Chen will serve as the center's deputy director and lead its cellular engineering efforts. (
  • Fifth, the effects of physical factors on cellular activity must be determined in engineered tissues. (
  • Knowing these signals may shorten the iterations required to replace a tissue successfully and direct cellular activity and phenotype toward a desired end goal. (
  • NIDCR encourages research on cellular and molecular mechanisms of DOC tissue damage, degeneration, aging and regeneration. (
  • As well as being low-cost, the microfabrication technique is billed as being versatile, scalable, and generalisable, meaning that it could be adapted to create different types of tissue by replicating their particular structural, cellular and compositional features. (