Anterior polar cataracts in CS rats: a predictor of mature cataract formation. (1/148)

PURPOSE: The objective of this study was to characterize the morphology of the anterior opacities formed during recovery from posterior subcapsular cataract (PSC) in Royal College of Surgeons (RCS) rats. METHODS: Lenses from RCS rats at 8 and 12 weeks postnatal (n = 14 and 12, respectively) were examined under a dissecting microscope for the presence of anterior opacities. Lenses with anterior opacities were fixed, embedded in epoxy resin, and sectioned along the optic axis for light microscopy (LM) and transmission electron microscopy (TEM). RESULTS: At eight weeks postnatal, 21.5% of animals (3/14) had anterior cataracts. Light microscopy of 1- to 2-microm-thick sections revealed an anomalous layer of material located at the epithelium-fiber interface, which was identified as a zone of liquefaction by TEM. Epithelial cells had minor structural defects but were not necrotic. Anterior portions of elongating and cortical fibers under the zone of liquefaction were undisrupted, whereas their posterior portions had numerous vacuoles. The anterior opacities were classified as anterior polar cataracts (APCs) based on the location and type of morphologic damage in the affected lenses. At twelve weeks postnatal, 25% of animals (3/12) had APCs that involved prominent vesiculation of the anterior cortex. Ultrastructural examination showed that large vesicles were located between and inside anterior fibers and that most extracellular spaces were abnormally widened. Posteriorly, internalization of the PSC by new fiber growth was disordered and displayed vesiculation and density variations. In the bow region, LM revealed minor structural irregularities that were identified as groups of apparently degenerating fibers by TEM. CONCLUSIONS: APCs in RCS rats are caused by degeneration of elongating fibers in the bow region and subsequent damage in the superficial anterior cortex. The percentage of animals with APCs (25%) was consistent with the percentage of animals in which mature cataracts eventually develop. The morphologic changes, time of onset, and percentage of animals affected suggest that APC is the initial manifestation of mature cataract formation in RCS rats.  (+info)

Localization of ubiquitin and ubiquitin cross-reactive protein in human and baboon endometrium and decidua during the menstrual cycle and early pregnancy. (2/148)

We have examined the distribution of ubiquitin and the related ubiquitin cross-reactive protein (UCRP) in paraffin-embedded sections of human and baboon endometrium and decidua by immunoperoxidase or immunofluorescence cytochemistry with antibodies raised against ubiquitin, UCRP, CD45, and insulin-like growth factor-binding protein-1. Anti-ubiquitin immunoreactivity was present in the nonpregnant endometrium, particularly in the glandular epithelial cells, and up-regulated in endometrial stromal cells as they decidualized at the beginning of pregnancy. Anti-UCRP immunoreactivity was absent from nonpregnant tissue but accumulated to high levels in decidual cells during pregnancy. Western blotting indicated that immunoreactivity was primarily due to the presence of ubiquitin and UCRP conjugated to other proteins, and that although levels of ubiquitin-protein conjugates do not change substantially during pregnancy, decidualization is accompanied by the appearance of conjugates of UCRP. Baboon uterine tissues demonstrated a similar distribution of the two proteins, which indicates that the baboon may be a useful model for study of the role of the ubiquitin system and UCRP in the establishment of pregnancy in humans.  (+info)

Senescence-associated beta-galactosidase histochemistry for the primate eye. (3/148)

PURPOSE: To develop a senescence-associated beta-galactosidase histochemistry and bleaching protocol for the primate posterior pole. METHODS: Rhesus monkey eyes of different ages were enucleated after death, fixed in 4% paraformaldehyde for up to 16 hours, and cryoprotected using a graded sucrose infiltration technique. Ten-micrometer tissue sections were treated with beta-galactosidase, pH 4 (lysosomal) or pH 6 (senescence-associated) activity, for various times. Bleaching of retinal pigment epithelial (RPE) cell and choroidal melanocyte pigment was performed after beta-galactosidase histochemistry using 0.1% to 1% potassium permanganate incubation for 1 minute to 2 hours followed by 0.5% oxalic acid immersion. RESULTS: A 6-hour incubation with beta-galactosidase, pH 4 or 6, demonstrated optimal staining of the RPE. Uniform staining of the RPE for pH 4 beta-galactosidase was seen in both young and old eyes. In contrast, senescence-associated beta-galactosidase (pH 6) staining was seen in the RPE of 16 and 29-year-old, but not 1- and 2-year-old eyes. Senescence-associated beta-galactosidase staining was evident in RPE cells adjacent to cuticular drusen. Optimal bleaching without loss of beta-galactosidase staining was obtained using a 25-minute incubation with 0.05% permanganate. CONCLUSIONS: The senescence-associated beta-galactosidase histochemistry assay, adapted for use in the primate posterior pole, showed staining of RPE cells in older eyes. Visualization of beta-galactosidase activity in the RPE was enhanced by permanganate bleaching of melanin pigment. This technique could be valuable for identifying senescent RPE cells in human eyes.  (+info)

Loss of PTEN expression in paraffin-embedded primary prostate cancer correlates with high Gleason score and advanced stage. (4/148)

The tumor suppressor gene PTEN/MMAC-1/TEP-1 (referred to hereafter as PTEN) maps to chromosome 10q23 and encodes a dual specificity phosphatase. The PTEN protein negatively regulates cell migration and cell survival and induces a G1 cell cycle block via negative regulation of the phosphatidylinositol 3'-kinase/protein kinase B/Akt signaling pathway. PTEN is frequently mutated or deleted in both prostate cancer cell lines and primary prostate cancers. A murine polyclonal antiserum was raised against a glutathione S-transferase fusion polypeptide of the COOH terninus of PTEN. Archival paraffin tissue sections from 109 cases of resected prostate cancer were immunostained with the antiserum, using DU145 and PC-3 cells as positive and negative controls, respectively. PTEN expression was seen in the secretory cells. Cases were considered positive when granular cytoplasmic staining was seen in all tumor cells, mixed when areas of both positive and negative tumor cell clones were seen, and negative when adjacent benign prostate tissue but not tumor tissue showed positive staining. Seventeen cases (15.6%) of prostate cancer were positive, 70 cases (64.2%) were mixed, and 22 cases (20.2%) were negative. Total absence of PTEN expression correlated with the Gleason score (P = 0.0081) and correlated more significantly with a Gleason score of 7 or higher (P = 0.0004) and with advanced pathological stage (American Joint Committee on Cancer stages T3b and T4; P = 0.0078). Thus, loss of PTEN protein is correlated with pathological markers of poor prognosis in prostate cancer.  (+info)

Morphological adaptation of the cardiovascular system in fetal rats during late gestation. (5/148)

The aim of this study was to evaluate morphological changes of the cardiovascular system in fetal rats during late gestation. We used the rapid whole-body freezing technique for rats of day 17 through 21 of gestation. The right and left ventricular volumes increased markedly and significantly during this period by about 11- and 24-fold, respectively. Although the right ventricular volume was 108% larger with statistical significance than the left ventricular volume on day 17, they were almost equal after day 19. The length of the primum septum of the atrium significantly increased by 92% within 4 days, but the opening distance of foramen ovale significantly decreased by 14%. The ratio of the inner diameter (the sum of right and left pulmonary arteries to ductus arteriosus) significantly increased from 0.72+/-0.03 on day 17 to 1.17+/-0.07 on day 21. There was also a significant increase in the ratio of the inner diameters of the ascending to descending aorta. These observations suggest that the reduction of the opening distance of foramen ovale reflect the growth of pulmonary arteries.  (+info)

Reappraisal of potassium permanganate oxidation applied to Lowicryl K4M embedded tissues processed by high pressure freezing/freeze substitution, with special reference to differential staining of the zymogen granules of rat gastric chief cells. (6/148)

The high pressure freezing/freeze substitution technique is known to yield a deep vitreous freezing of tissues. Combination of this technique with Lowicryl K4M embedding allows us histochemical studies of dynamic cellular processes with improved structural preservation. The disadvantage of Lowicryl K4M embedding is its poor electron density in electron microscopy. To address this problem, we examined the effects of KMnO4 oxidation applied to Lowicryl K4M embedded rat gastric glands processed by high pressure freezing. The KMnO4 oxidation-uranyl acetate-lead citrate sequence succeeded not only in contrast enhancement of cellular components, but also in differential staining of the zymogen granules of rat gastric chief cells. This technique could be applied to semi-thin sections of Lowicryl K4M embedded rat gastric glands. The KMnO4 oxidation-toluidine blue staining provided sufficient contrast with regard to the zymogen granules. Various experiments used in this study verified that the KMnO4 oxidation plays an essential role in the differential staining of the zymogen granules. Combined use of the KMnO4 oxidation with phospholipase A2-immunostaining demonstrated that gold labeling was localized to the zymogen granules without the loss of immunolabeling. Energy dispersive X-ray microanalysis revealed some manganese depositions on the zymogen granules. It is highly anticipated that the KMnO4 oxidation will become a useful tool for histochemical investigations combined with cryofixation/freeze substitution and low temperature embedding techniques.  (+info)

Widefield deconvolution epifluorescence microscopy combined with fluorescence in situ hybridization reveals the spatial arrangement of bacteria in sponge tissue. (7/148)

Widefield deconvolution epifluorescence microscopy (WDEM) combined with fluorescence in situ hybridization (FISH) was performed to identify and characterize single bacterial cells within sections of the mediterranean sponge Chondrosia reniformis. Sponges were embedded in paraffin wax or plastic prior to the preparation of thin sections, in situ hybridization and microscopy. Serial digital images generated by widefield epifluorescence microscopy were visualized using an exhaustive photon reassignment deconvolution algorithm and three-dimensional rendering software. Computer processing of series of images taken at different focal planes with the deconvolution technique provided deblurred three-dimensional images with high optical resolution on a submicron scale. Results from the deconvolution enhanced widefield microscopy were compared with conventional epifluorescent microscopical images. By the application of the deconvolution algorithm on digital image data obtained with widefield epifluorescence microscopy after FISH, the occurrence and spatial arrangement of Desulfovibrionaceae closely associated with micropores of Chondrosia reniformis could be visualized.  (+info)

Structure of cytomatrix and nuclear matrix revealed by embedment-free electron microscopy. (8/148)

Embedment-free electron microscopy (EFEM) is a new method which allows the visualisation of cytoskeleton in whole-mounted cells. In this study we employed EFEM to investigate the structure of cellular scaffolds in glioma C6 cell line. The cells were extracted with Triton X-100 that dissolves phospholipids in the membranes and removes most of cytoplasmic soluble proteins. The DNA and nuclear histones were removed with DNase I and high-salt buffer, respectively. The remaining cellular frameworks were temporary embedded in diethylene glycol distearate (DGD), sectioned and observed in transmission and scanning electron microscope after the removal of DGD. The predominant structure was the extensive meshwork of 10-20 nm filaments in the cytoplasm (cytomatrix) and 15-30 nm filaments in the nucleus (nuclear matrix). The 5 nm filaments, presumably corresponding to the actin filaments, were present in the cytomatrix, but not in the nuclear matrix. Moreover, the ultrathin (3 nm) filaments, connecting other cytoskeletal components were detected. Those are possibly identical with the previously described plectin filaments. For the first time we report the occurrence of ultrathin filaments in the nuclear matrix. Thus, in a addition to the well known cytoskeletal components (microtubules, intermediate filaments, actin microfilaments) EFEM showed a new type of filaments (the ultrathin filaments) in the cytomatrix and nuclear matrix. Further immunocytochemical studies are needed to determine the biochemical identity of the filaments observed in EFEM.  (+info)

... is the insertion of foreign objects either into soft tissues under the skin or into muscle. Self-embedding is ... He also embedded needles into his abdomen. In 1986 Gould and Pyle described self-embedding behavior in their book Anomalies and ... The majority of people who engage in self-embedding are white teenage females with psychiatric diagnoses. Self-embedding has a ... Other distinctions between self-embedding and other self-injurious behaviors are that self-embedding is highly comorbid with ...
The test is run on formalin fixed, paraffin-embedded tissue. MammaPrint traditionally used rapidly frozen tissue but a room ... The test is run on formalin fixed, paraffin-embedded tissue. Oncotype results are reported as a Recurrence Score (RS), where a ... October 2005). "Gene expression profiles in paraffin-embedded core biopsy tissue predict response to chemotherapy in women with ... Carcinoma in situ is proliferation of cancer cells within the epithelial tissue without invasion of the surrounding tissue. In ...
They are solitary and embedded into the host plant tissue. A pseudothecium has small dark hairs around its opening, and ... In addition to direct removal of fallen infected tissues, mulching of fallen leaves into the soil to destroy the ascospores is ... Scab lesions located on the woody tissues may also overwinter in place, but will not undergo a sexual reproduction cycle; these ... A fungal mycelium forms between the cuticle and underlying epidermal tissue, developing asexually the conidia, that germinate ...
Fixed or fresh tissue pieces are embedded in low gelling temperature agarose. The resulting agarose block containing the tissue ... No need to dehydrate tissues prior to embedding, thus decreased loss of cell constituents No messy paraffin embedding Allows ... Preparing the specimen Specimen preparation includes fixation and embedding as needed. Adjusting the thickness Tissue location ... Decreased tissue autofluorescence due to avoidance of formalin-fixation and paraffin embedding Less wait period from tissue ...
Engbaek, K; Johansen, KS; Jensen, ME (February 1979). "A new technique for Gram staining paraffin-embedded tissue". Journal of ... Acetone is used in the field of pathology to find lymph nodes in fatty tissues for tumor staging (such as looking for lymph ... in which acetone is formed in body tissue. Certain health conditions, such as alcoholism and diabetes, can produce ketoacidosis ...
These cells are embedded within collagen bundles which generally dominate the tumor tissues. Compared to GF tumor tissue, DT ... the predominant structural protein in connective tissue). Slit-like empty spaces and loci of adipose tissue and/or skeletal ... Sbaraglia M, Bellan E, Dei Tos AP (April 2021). "The 2020 WHO Classification of Soft Tissue Tumours: news and perspectives". ... Baranov E, Hornick JL (March 2020). "Soft Tissue Special Issue: Fibroblastic and Myofibroblastic Neoplasms of the Head and Neck ...
Tissue embedded samples can also be examined if thinly sectioned. Sample preparations vary depending on protocol and user but ... The TCID50 (50% Tissue Culture Infectious Dose) assay is the measure of infectious virus titer. This endpoint dilution assay ... The FFA method typically yields results in less time than plaque assays or fifty-percent-tissue-culture-infective-dose (TCID50 ... of inoculated tissue culture cells. This assay may be more common in clinical research applications where the lethal dose of ...
They are embedded in the tissue, dark brown and globular. The conidia come in two types, but the most common is beta conidia ... At the end of the season, perithecia formed on leaves and other diseased tissues will remain in the debris and survive for the ... Perithecia are found on cortical tissues and will produce globular to sub-cylindrical asci with two-celled ascospores. It has ... Pycnidia, the anamorphic fruiting body, will form in the infected tissue and release two types of conidia - alpha and beta. ...
... cell cultures and aliquots of solid tissue. Frozen and paraffin embedded archival tissue may also be used. Emmert-Buck MR, ... An IR laser gently heats the adhesive on the cap fusing it to the underlying tissue and an UV laser cuts through tissue and ... The membrane-tissue entity now adheres to the cap and the cells on the cap can be used in downstream applications (DNA, RNA, ... And the system uses a 355 nm Solid-state laser(UV-A) which is the safest way to cut the tissues without RNA or DNA damage.[ ...
Many are found embedded in the tissues of the white encrusting sponge Geodia gibberosa. Male individuals, being motile, are ...
"Fluorescence in Situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays". ... Tissue samples are securely attached to a surface, which is usually a glass slide, with paraffin. The tissue samples must then ... Another advantage of using bright-field microscopy is that the tissue or cell sample as a whole can be visualized through CISH ... A novel alternative in screening archival breast cancer tissue samples for HER-2/neu status". Breast Cancer Research. 6 (5): ...
They contain numerous nuclei which lie embedded in the thin lining layer of protoplasm. They are irregularly distributed in the ... These tissues are classified as either laticiferous tissues or glandular tissues. Cells or organizations of cells which produce ... They may also act as food storage organs or as reservoir of waste products, or as translocatory tissues. This tissue consists ... The tissues that are concerned with the secretion of gums, resins, volatile oils, nectar latex, and other substances in plants ...
"Glycol Methacrylate in Light Microscopy a Routine Method for Embedding and Sectioning Animal Tissues". Stain Technology. 49 (6 ... doi:10.1016/0032-3861(82)90020-9. Rosenberg, M.; Bartl, P.; Lesko, J. (1960). "Water-soluble methacrylate as an embedding ... Gerrits, P. O.; Horobin, R. W. (1996). "Glycol Methacrylate Embedding for Light Microscopy: Basic Principles and Trouble- ... In electron microscopy, later in light microscopy, hydroxyethylmethacrylate serves as an embedding medium. When treated with ...
It can become completely embedded in the root and become an endoparisite. Necrotic tissue is often found as a result of the ... H. Mangiferae anterior becomes embedded in the cortex of the root. It is typically an ectoparasite of the root. ...
See also freeze drying.[citation needed] Embedding, biological specimens - after dehydration, tissue for observation in the ... tissues may also be embedded directly in water-miscible acrylic resin. After the resin has been polymerized (hardened) the ... The fresh tissue or cell suspension is frozen rapidly (cryofixation), then fractured by breaking (or by using a microtome) ... transmission electron microscope is embedded so it can be sectioned ready for viewing. To do this the tissue is passed through ...
Lau SK, Chu PG, Weiss LM (November 2004). "CD163: a specific marker of macrophages in paraffin-embedded tissue samples". ...
... can become embedded in animals' tissue through ingestion or respiration. Various annelid species, such as deposit ... become embedded in animal tissue, and are then undetectable by unaided visual inspection. Furthermore, consequences of plastic ... Microplastics are also found within the many other types of marine particles such as dead biological material (tissue and ... Microplastics were found in every human tissue studied by graduate students at Arizona State University. A study published in ...
A cryostat takes fresh or fixed tissue and immerses it into liquid nitrogen for flash freezing. Then tissue is embedded in ... The Compresstome cuts tissue into thin slices without a freeze process; free-floating sections are cut after being embedded in ... In addition, in-situ hybridization on tissue sections require that tissue slices be very thin, usually 3 µm to 7 µm in ... or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells, and ...
... paraffin-embedded cancer tissue". Proceedings of the National Academy of Sciences. 110 (29): 11982-11987. Bibcode:2013PNAS.. ...
... paraffin-embedded cancer tissue". Proceedings of the National Academy of Sciences. 110 (29): 11982-11987. Bibcode:2013PNAS.. ... "An improved method for discrimination of cell populations in tissue sections using microscopy-based multicolor tissue cytometry ... Detection of 2000 Cell Surface Protein Clusters in a Single Tissue Section and Cell Type Specific Annotation by Using a Three ... The ICM is the core technology for the systematic mapping of the complete protein network code in tissues (human toponome ...
"Beam deceleration for block-face scanning electron microscopy of embedded biological tissue". Micron. 43 (5): 612-620. doi: ... Lee, Joung-Hyun; Gu, Yexin; Wang, Hongjun; Lee, Woo Y. (2012). "Microfluidic 3D bone tissue model for high-throughput ... "3-D illustration of network orientations of interstitial cells of Cajal subgroups in human colon as revealed by deep-tissue ... a multiscale porous media study from cellular to tissue scales, P S. Takhar, Department of Animal and Food Sciences, ...
This process is called embedding. The substance used to embed tissue is embedding media, which is chosen depends on the ... A tissue specimen can keep for several years after finishing embedding this tissue into the wax. Paraffin wax, which is soft ... embed and slice the tissue with celloidin. Moreover, this slicing method can slice large tissues and has the advantage that its ... Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. Since few plant tissues have a color, ...
... which may also be known as detergent or hydrogel embedding methods. Tissue-expansion clearing methods use hydrogel, and may be ... Tissue clearing has also been applied to human cancer tissues Zhao J, Lai HM, Qi Y, He D, Sun H (January 2021). "Current Status ... Tissue clearing refers to a group of chemical techniques used to turn tissues transparent. This allows deep insight into these ... Tissue opacity is thought to be the result of light scattering due to heterogeneous refractive indices. Tissue clearing methods ...
... entirely surrounded by pulp tissue 2) Adherent: partly fused with dentin 3) Embedded: entirely surrounded by dentin Pulp stones ... Embedded pulp stone is fully embedded in dentine and most commonly found in the apical portion of the root. Adherent pulp ... Free pulp stones are found within the pulp tissue and is the most common encounter. The size vary from 50μm in diameter to ... It is believed that pulp stones develop around a central nidus of pulp tissue, for instance collagen fibril, ground substance ...
It is surrounded by a ring of small plates overlaid by softer tissue known as the peristome. Embedded in the peristome are five ...
MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissue Enables the Delineation of Ovarian Cancer Tissues". ... "Unlocking Cancer Glycomes from Histopathological Formalin-fixed and Paraffin-embedded (FFPE) Tissue Microdissections". ... and human tissues, but have subsequently been found also to decorate non-lysosomal glycoproteins. Particularly, the granules of ... In humans, the Hex-mediated GnT-I-dependent truncation pathway is known to facilitate, at least in some tissues including ...
SCS are embedded in tissue and persist in differentiated areas throughout tumor progression. MCS are located at the tumor-host ... This is most often associated with tissues with a high rate of cell turnover (such as the skin or gut). In these tissues, ASCs ... Hence, at least for certain neoplastic tissues, dedicated stem cell compartments maintain and enlarge the size of the ... Many normal stem cells such as hematopoietic or stem cells from tissues, under special culture conditions, form three- ...
Embedded in the coenenchyme are minute capstan-shaped and rod-shaped calcified sclerites. As well as strengthening the tissue, ... The sea whip slug (Tritonia wellsi) feeds on the soft tissues of the sea fan. It is difficult to detect because it is disguised ... a thin layer of living tissue from which the polyps protrude. ...
Inside the yellow margin are conspicuous white mantle glands embedded in the tissue. The rhinophores and gills are purple-pink ...
Many antigens can be successfully demonstrated in formalin-fixed paraffin-embedded tissue sections. However, some antigens will ... Tissue preparation or fixation is essential for the preservation of cell morphology and tissue architecture. Inappropriate or ... Under these conditions, tissues should be rapidly fresh frozen in liquid nitrogen and cut with a cryostat. The disadvantages of ... The disadvantage of vibratome sections is that the sectioning process is slow and difficult with soft and poorly fixed tissues ...
Histosette II Tissue Processing/Embedding Cassettes with Combination Lid and Base - Open and close as often as necessary, ... Embedding Cassettes and Storage * Fisherbrand™ Histosette™ II Tissue Processing/Embedding Cassettes with Combination Lid and ... Histosette™ II Tissue Processing/Embedding Cassettes with Combination Lid and Base open and close as often as necessary - ... Fisherbrand™ Histosette™ II Tissue Processing/Embedding Cassettes with Combination Lid and Base ...
"Tissue Embedding" by people in Harvard Catalyst Profiles by year, and whether "Tissue Embedding" was a major or minor topic of ... "Tissue Embedding" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Tissue Embedding" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Tissue Embedding". ...
II Tissue Processing/Embedding Cassettes at Thomas Scientific, your trusted partner in Science. ... HISTOSETTE® II Tissue Processing/Embedding Cassettes. * PRODUCT AVAILABILITY: Did you know you can view a products ... Disposable plastic cassettes hold tissue specimens during processing and embedding, as well as in storage. Molded from a ...
Mega-Cassette embedding cassettes have been designed to accommodate a variety of large specimens, including bone, eye, teeth ... Tissue Embedding Accessories Embedding Cassettes Embedding Media Embedding Moulds Fixatives Handling and Tools Sealing Film Wax ... "Tissue-Tek Mega-Cassette embedding cassettes - 30 x 24 x 11mm \/ Pack\/125","public_title":"30 x 24 x 11mm \/ Pack\/125"," ... "Tissue-Tek Mega-Cassette embedding cassettes - 30 x 24 x 11mm \/ Pack\/125","public_title":"30 x 24 x 11mm \/ Pack\/125"," ...
... Pathol Res ... We analyzed the specificity of the antibody on a panel of normal and neoplastic tissues, and we assessed its sensitivity in a ... T311 is a specific and sensitive marker for the detection of melanocytic lesions in formalin-fixed paraffin-embedded tissues ... T311 revealed intense reactivity on paraffin-embedded material. Immunoreactivity was limited to cells of melanocytic ...
Self-Embedding Disorder and Removal of Soft Tissue Foreign Bodies Figure 1 (Young et al., 2008). This x-ray image illustrates 3 ... In cases of self-embedding disorder, objects are used to puncture the skin or are embedded into the wound after cutting.. ... Self-Embedding Disorder appears to be a newly-coined term1 described in a press release issued by the Radiological Society of ... Figure 2 (Young et al., 2008). This x-ray image illustrates 8 metal pieces embedded in the left arm of a teenage girl.. ...
Context: While formalin fixation and paraffin embedding has become a universal mechanism of tissue preservation and a gold ... paraffin-embedded tissue Arch Pathol Lab Med. 2011 May;135(5):537-43. doi: 10.5858/2010-0702-RAIR.1. ...
We successfully detected T. whipplei in tissue biopsies with a sensitivity of 83% in untreated (5/6) and 40% in treated (4/10) ... We successfully detected T. whipplei in tissue biopsies with a sensitivity of 83% in untreated (5/6) and 40% in treated (4/10) ... 19 formalin-fixed paraffin-embedded (FFPE) duodenal biopsy specimens of 12 patients with treated (6/12) and untreated (6/12) WD ... 19 formalin-fixed paraffin-embedded (FFPE) duodenal biopsy specimens of 12 patients with treated (6/12) and untreated (6/12) WD ...
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Active Motif has leveraged its epigenetics expertise to develop unique kits that enable ChIP from FFPE tissue for use in Next ... The tissue then undergoes an incubation in lysis buffer, tissue homogenization, a 30 second sonication followed by digestion ... The paraffin is removed and the tissue is rehydrated from each slide/tube. The tissue then undergoes a sonication. A final ... The ChIP-IT® FFPE II Kit requires chromatin prepared from FFPE slides or tissue blocks using the ChIP-IT® FFPE Chromatin ...
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One-step embedding method for maintaining orientation of pathological tissue specimens using agar thin films ... One-step embedding method for maintaining orientation of pathological tissue specimens using agar thin films ... One-step embedding method for maintaining orientation of pathological tissue specimens using agar thin films ...
Protein extraction from methanol fixed paraffin embedded tissue blocks: A new possibility using cell blocks ... Normal (Stomach, Colon) (n = 8/tissue group) and malignant (Colon, Kidney) (n = 3-4/tissue group) tissue specimens were ... These processed blocks containing the scrapings and tissues were termed as C-MFPE tissue scraping blocks and as C-MFPE tissue ... followed-by tissue processing - 10 h and paraffin embedding - 20 min. The scrapings and the tissue aliquots collected into ...
Connective tissue is the non-living intracellular material in which the tissues remain embedded. - 76x4ttt ... Connective tissue is the non-living intracellular material in which the tissues remain embedded. ... Name the non-living intracellular material in which the tissues remain embedded. ... A bundle containing complex permanent tissue d) Tissue responsible for conduction of water (NOTE: The following picture will ...
Unfortunately we dont have an oven in the lab where the embedding equipment is. Our Tissue Tek II embedding station has a ... Our concern was keeping the tissue warm so that we could embed quickly when needed without having to warm the tissue up. We ... Histonet] Tissue transport from processor to embedding station. Maria Christensen mariac ,@t, creighton.edu Fri Sep 10 10:04:40 ... Next message: [Histonet] Tissue transport from processor to embedding station * Messages sorted by: [ date ] [ thread ] [ ...
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Evaluation of the Novaplex II HPV28 Detection Assay for HPV Typing in Formalin-Fixed Paraffin-Embedded Tissues Cite ... 2023). Evaluation of the Novaplex II HPV28 Detection Assay for HPV Typing in Formalin-Fixed Paraffin-Embedded Tissues. 25(4). ... "Evaluation of the Novaplex II HPV28 Detection Assay for HPV Typing in Formalin-Fixed Paraffin-Embedded Tissues" vol. 25, no. 4 ... Title : Evaluation of the Novaplex II HPV28 Detection Assay for HPV Typing in Formalin-Fixed Paraffin-Embedded Tissues Personal ...
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  • Formalin -fixed paraffin -embedded tissue samples were collected and archived from autopsies conducted over 13 years in the forensic medicine department of the University of Pretoria (Pretoria, South Africa ). (bvsalud.org)
  • Mega-Cassette embedding cassettes have been designed to accommodate a variety of large specimens, including bone, eye, teeth etc. (proscitech.com.au)
  • We analyzed the specificity of the antibody on a panel of normal and neoplastic tissues, and we assessed its sensitivity in a large number of metastatic and primary malignant melanomas, nevi, three angiomyolipomas, and two vitiligo specimens. (nih.gov)
  • Thresholds identified in the literature were then compared with published immunohistochemistry guidelines for formalin-fixed, paraffin-embedded specimens. (nih.gov)
  • 19 formalin-fixed paraffin-embedded (FFPE) duodenal biopsy specimens of 12 patients with treated (6/12) and untreated (6/12) WD were retrospectively examined using PAS diastase staining, immunohistochemistry, and FISH. (frontiersin.org)
  • MFPE blocks were made by Cellient™ automated system using human tissue specimens from normal and malignant specimens collected in ThinPrep™ Vials. (cytojournal.com)
  • In the current era of minimally invasive techniques for obtaining tissue samples for diagnostic and prognostic markers, cellblocks containing "limited material" specimens are routinely used to provide the valuable information about pre-disease and disease processes. (cytojournal.com)
  • We established that FFPE sections retain a similar phosphoproteome to fresh tissue specimens during storage for at least 9 months, confirming the utility of our method for evaluating phosphorylation profiles in various diseases. (figshare.com)
  • Tissue Processing Cassettes provide a modular, organized method for safely and consistently handling, embedding, partitioning, and storing specimens. (superfirt.com)
  • Formalin -fixed paraffin -embedded (FFPE) tissue archives in hospitals , biobanks, and others offer a vast collection of extensive, readily available specimens for molecular testing . (bvsalud.org)
  • This Sakura Tissue-Tek® Tec™ 4 Embedding Station is fully refurbished and in excellent condition. (histologyequipment.com)
  • The Sakura Tissue Tek TEC 4 Tissue Embedding Center is economically priced and truly refurbished. (histologyequipment.com)
  • Sakura Tissue-Tek TEC 5 Tissue Embedd. (newlifescientific.com)
  • Elevate your histology lab with the Sakura Tissue-Tek TEC 6 Embedding Station, a hallmark of reliability and comfort. (rankinbiomed.com)
  • Histosette™ II Tissue Processing/Embedding Cassettes with Combination Lid and Base open and close as often as necessary - cassettes will always relock securely. (fishersci.com)
  • Our Tissue Tek II embedding station has a heated compartment for the molds, but no compartment for the cassettes. (utsouthwestern.edu)
  • Two heated trays provide a pre-warming capacity of 80 cassettes and 200 embedding moulds. (kalstein.ee)
  • Tissue Embedding Cassettes, which designed to eliminate "chatter" during segmentation. (superfirt.com)
  • While formalin fixation and paraffin embedding has become a universal mechanism of tissue preservation and a gold standard for immunohistochemistry, fixation and processing variables that may confound assay effectiveness have received little attention from the scientific community. (nih.gov)
  • This IHC protocol provides a basic guide for the fixation, microtome sectioning, and staining of paraffin-embedded tissue samples. (rndsystems.com)
  • After fresh-tissue harvest, the prostate gland is reassembled and sutured using metal skin staples to avoid distortion of the specimen during fixation. (medscape.com)
  • Optimal fixation is critical for the histologic assessment of prostate cancer , and most artifacts related to the histologic or immunohistologic evaluation are a manifestation of poor tissue fixation. (medscape.com)
  • Even today, pathologists still follow the same basic steps to prepare and investigate tissue: chemical fixation, slicing, staining and microscopy. (newswise.com)
  • Imaging of tissue samples without fixation or embedding is of interest in studies of meniscus biomechanics. (lu.se)
  • Formalin-fixed paraffin-embedded (FFPE) clinical samples are a valuable resource for retrospective research. (activemotif.com)
  • Active Motif has leveraged its epigenetics expertise to develop commercially available kits to enable ChIP from FFPE tissue for use in Next Generation Sequencing (ChIP-Seq). (activemotif.com)
  • The ChIP-IT ® FFPE Chromatin Preparation Kits contain specially formulated reagents and protocol guidelines to extract high quality chromatin from histological slides or tissue sections. (activemotif.com)
  • In ChIP-IT ® FFPE Chromatin Preparation, 10-20 µm sections from FFPE tissue blocks, or 5-10 µm sections from unstained FFPE slides are used as the starting material. (activemotif.com)
  • Advances in the field of molecular biotechnology have made it possible to extract proteins from formalin fixed and paraffin embedded (FFPE) tissue blocks. (cytojournal.com)
  • Protein was extracted from Cellient-methanol fixed and paraffin embedded blocks with CHAPS buffer method as well as FFPE and Mammalian Qiagen ® kits. (cytojournal.com)
  • Despite advances in technology, the relatively small size of the cytology scrapings MFPE cell blocks in comparison to the formalin fixed and paraffin embedded (FFPE) counterparts have caused them to be often overlooked in biomarker discovery. (cytojournal.com)
  • Formalin-fixed and paraffin-embedded (FFPE) sections mounted on microscope slides are one of the largest available resources for retrospective research on various diseases, but quantitative phosphoproteome analysis of FFPE sections has never been achieved because of the extreme difficulty of procuring sufficient phosphopeptides from the limited amounts of proteins on the slides. (figshare.com)
  • Liver micro- proteomics based on the routinely used formaldehyde -fixed paraffin -embedded (FFPE) samples is valuable for innovative research , but the technical approach for sample preparation is often challenging. (bvsalud.org)
  • thus, the forensic pathology FFPE tissue archives in Africa have been a largely unexploited genetic resource , with the usability of DNA obtainable from these samples being unknown.Intervention The study, conducted from January 2015 to August 2016, determined the usefulness of FFPE tissue as a reliable source of genetic material for successful post-mortem molecular applications and diagnostics. (bvsalud.org)
  • Deoxyribonucleic acid from FFPE tissue samples and control blood samples was amplified by high-resolution melt real-time polymerase chain reaction before sequencing. (bvsalud.org)
  • Paraffin embedding of tissue is a common technique used in histology and pathology to preserve and prepare tissue samples for microscopic examination. (ibiospecimen.com)
  • With their new imaging approach, these changes can be visualized for the first time in larger tissue volumes, without cutting and staining or damaging the tissue as in conventional histology. (newswise.com)
  • Heavy metal staining of paraffin epoxy and glycol methacrylate embedded biological tissue for scanning electron microscope histology. (cdc.gov)
  • Assessment using a wide variety of normal and neoplastic tissue indicates reactivity with all tissues of simple or glandular epithelial origin, and in addition with many squamous carcinomas. (ox.ac.uk)
  • Please review the photos to make sure the current physical condition of the embedding module and cryo module is to your standards. (newlifescientific.com)
  • Methyl methacrylate embedding to study the morphology and immunohistochemistry of adult guinea pig and mouse cochleae. (harvard.edu)
  • The following immunohistochemistry (IHC) protocol has been developed and optimized by R&D Systems' IHC/ICC laboratory for chromogenic IHC experiments using paraffin-embedded tissue samples. (rndsystems.com)
  • Once the tissue has been sectioned and stained, it can be further analyzed using immunohistochemistry, molecular testing, or other specialized techniques. (ibiospecimen.com)
  • Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. (blunck-medical-books.de)
  • NCL-5D3: a new monoclonal antibody recognizing low molecular weight cytokeratins effective for immunohistochemistry using fixed paraffin-embedded tissue. (ox.ac.uk)
  • Pre-embedding immunogold labeling to optimize protein localization at subcellular compartments and membrane microdomains of leukocytes. (harvard.edu)
  • We conclude that T311 is a specific and sensitive marker for the detection of melanocytic lesions in formalin-fixed paraffin-embedded tissues and a useful serological reagent for diagnostic pathology. (nih.gov)
  • It is designed for use in pathology laboratories and for embedding histological tissue samples only in molten paraffin for the following tasks: To embed the sample, melt the solid paraffin and keep the melted paraffin at the required temperature. (ukaelab.com)
  • Slides are pre-coated with gelatin to enhance adhesion of the tissue. (rndsystems.com)
  • Please refer to the Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections for instructions on how to prepare gelatin-coated slides. (rndsystems.com)
  • Slides with paraffin-embedded sections can be stored either at room temperature or in at 2-8 °C for several years in slide storage boxes. (rndsystems.com)
  • This embedded tissue block is then sliced into thin sections using a microtome and mounted onto glass slides. (ibiospecimen.com)
  • 2.Independently developed the first printer that can print both embedding cassette and slide in the world. (xwlabware.com)
  • Our laser printer series includes embedding cassette printer, slide printer and cassette & slide 2 in 1 printer, which can meet the different needs of the lab. (xwlabware.com)
  • Meanwhile, we pioneered the concept of double channel printing, the embedding cassette or slide can be printed alternately at the same time. (xwlabware.com)
  • It is also the first printer in the world that can print the embedding cassette and slide at the same time. (xwlabware.com)
  • Superfirt's Histopathology Tissue Cassette is suitable for use with routine tissue samples. (superfirt.com)
  • This study was performed to evaluate T311 as a diagnostic immunohistochemical reagent for use on formalin-fixed paraffin-embedded pathological material. (nih.gov)
  • In the current era of minimally invasive techniques to obtain minimal amount of tissue for diagnostic and prognostic purposes, the use of commercial and lab made buffer on low weight MFPE scrapings obtained by Cellient ® processor opens new possibilities for protein biomarker research. (cytojournal.com)
  • In USA, there are several laboratories and hospitals that offer blood tests and diagnostic services that may involve the use of paraffin embedded tissue. (ibiospecimen.com)
  • This burnt orange Histo Plas™ Uni-Capsette™ with detachable plastic lid provides a modular, organized means for safe and consistent processing, embedding, sectioning, and storage of specimen samples. (capitolscientific.com)
  • Embed the tissue in paraffin at 58 °C. Tissues can be embedded into paraffin using specialized automated tissue processing systems. (rndsystems.com)
  • The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins. (harvard.edu)
  • Tissues were embedded in paraffin, epoxy (EponAraldite), and butoxyethanol-glycol methacrylate (BGMA). (cdc.gov)
  • Epoxy embedded tissue was postfixed in Os04, mounted on carbon-coated nylon grids and stained with uranyl acetate and lead citrate. (cdc.gov)
  • Epoxy embedding - for the best intracellular details and tissue patterns, without interference from grid bars. (cdc.gov)
  • We investigated commonly available CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate) buffer, as well as two commercially available Qiagen ® kits and compared their effectiveness on MFPE tissue for protein yields. (cytojournal.com)
  • Paraffin embedded tissue is commonly used in histopathology, a branch of medicine that involves the microscopic examination of tissue samples. (ibiospecimen.com)
  • The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. (harvard.edu)
  • Cut 5 - 15 µm thick tissue sections using a rotary microtome. (rndsystems.com)
  • High Capacity for High Efficiency.Integrated ParaTrimmer.Only workstation with a heated wax trimmer built directly into the workspace.Remove excess paraffin at the embedding station, eliminating the need for additional equipment or wax scraping at the microtome. (xwlabware.com)
  • Paraffin embedded tissue refers to a method of preserving tissue samples for examination and analysis in a laboratory setting. (ibiospecimen.com)
  • This means that tissue samples can be stored for future research or reevaluation, even years after the initial diagnosis. (ibiospecimen.com)
  • Polyester fibers, a common byproduct of laundry machines, were the most common in tissue samples, as was polyethylene, which is a component of beverage containers. (curbearth.com)
  • But real attempts to extract DNA from amber or similar substances were unsuccessful to this day, and resin-embedded samples were deemed unsuitable for genetic examinations. (forbes.com)
  • Only after carefully cleaning the samples and repeating the PCR-process various times, enough DNA was replicated to study the genomics of the embedded organism. (forbes.com)
  • Since the start of the outbreak in September 2012, CDC and State health laboratories have tested tissue and fluid samples from patients with probable or confirmed fungal infection. (cdc.gov)
  • Development of a sample preparation method for micro-proteomics analysis of the formaldehyde-fixed paraffin-embedded liver tissue samples. (bvsalud.org)
  • Conclusion: Dilution of the DNA extracted of paraffin-embedded materials did not modify statistically the amount of positive samples β-globin gene amplified in PCR, although the results suggest that this is a way to increase the method for efficacy amplification of PCR. (bvsalud.org)
  • Name the non-living intracellular material in which the tissues remain embedded. (topperlearning.com)
  • Osmicated tissue shows good intracellular detail with SEM. (cdc.gov)
  • To preserve tissue morphology and retain the antigenicity of the target molecules, fix the tissue by vascular perfusion with 500 - 700 mL of Formaldehyde Fixative Solution. (rndsystems.com)
  • Our broad range of tissue processors means you can choose the right instrument for your laboratory's space, throughput, and workflow needs. (leicabiosystems.com)
  • It includes an embedding console and a cryoconsole that features solid-state electronics and a modular uniplane design configured for left-to-right or right-to-left workflow. (histologyequipment.com)
  • T311 revealed intense reactivity on paraffin-embedded material. (nih.gov)
  • Whipple's disease (WD) is a rare chronic systemic infection with a wide range of clinical symptoms, routinely diagnosed in biopsies from the small intestine and other tissues by periodic acid-Schiff (PAS) diastase staining and immunohistological analysis with specific antibodies. (frontiersin.org)
  • In this protocol, signal visualization is achieved using R&D Systems' Cell and Tissue Staining Kits . (rndsystems.com)
  • Specialized staining techniques are also employed to detect specific molecules or structures within the tissue. (ibiospecimen.com)
  • This staining process helps pathologists and other medical professionals examine the tissue under a microscope and make accurate diagnoses. (ibiospecimen.com)
  • Other distinctions between self-embedding and other self-injurious behaviors are that self-embedding is highly comorbid with behavioral health diagnoses and has a high prevalence of repetitive behavior. (wikipedia.org)
  • The majority of people who engage in self-embedding are white teenage females with psychiatric diagnoses. (wikipedia.org)
  • In order to assess self-embedding different aspects of the behavior must be examined such as the type of object used, the site of insertion, the number of objects inserted, the motivation behind the behavior and if the patient has other psychiatric diagnoses. (wikipedia.org)
  • Radiologists are in a unique position to be the first to detect self-embedding disorder, make the appropriate diagnosis and mobilize the healthcare system for early and effective intervention and treatment," said the study's principal investigator, William E. Shiels II, D.O., chief of the Department of Radiology at Nationwide Children's Hospital in Columbus, Ohio. (blogspot.com)
  • Your aim is to have an accurate orientation of the tissue to achieve quality sections, which enable you to make an accurate diagnosis. (sakura.eu)
  • Tissue embedding requires accurate orientation of the tissue to achieve sections that pathologists can use to make an accurate diagnosis. (sakura.eu)
  • It allows for detailed analysis of cellular and tissue structures, aiding in the diagnosis of various diseases, including cancer, infections, and inflammatory conditions. (ibiospecimen.com)
  • Tree resin with embedded ambrosia beetles. (forbes.com)
  • The researchers will apply the new method to other examples of resin-embedded insects, from most recent to the oldest one, to determine the decay rate of DNA. (forbes.com)
  • The resin creates a fully waterproof barrier, keeping moisture in the tissue. (forbes.com)
  • The interventional radiologists enter the scene (and intervene) when they use imaging to assist in the removal of self-inflicted soft tissue foreign bodies (STFBs). (blogspot.com)
  • To evaluate the efficacy and clinical impact of image-guided foreign body removal (IGFBR) for treatment of self-inflicted soft tissue foreign bodies (STFBs). (blogspot.com)
  • The novel and later successful movie franchise " Jurassic Park " popularized the idea that amber could preserve soft tissue and even DNA-molecules over millions of years. (forbes.com)
  • The objective of this work is to study the effect of carbon nanobrushes embedded within hydrogel composites on tissue engineering. (asme.org)
  • For instance, embedding nanomaterials into 3D printed composites can enhance the properties of these complex parts, extending composite capabilities for electrical/thermal gradients, increasing strength, and decreasing overall weight. (cdc.gov)
  • However, the resolution and contrast of this conventional technique are not sufficient to detect the tissue structure with cellular or sub-cellular resolution. (newswise.com)
  • The methods evaluated in this thesis have the potential to detect and follow tissue degeneration in articular cartilage and meniscus and could become valuable tools in future studies to increase our knowledge of disease progression in OA. (lu.se)
  • In this case, the tumor is macroscopically visible, allowing a fresh sample of malignant tissue to be harvested. (medscape.com)
  • Because paraffin is immiscible with water, tissue must be dehydrated before adding molten paraffin wax. (rndsystems.com)
  • The tissue is then immersed in molten paraffin wax, which infiltrates and impregnates the tissue, ensuring its preservation. (ibiospecimen.com)
  • Optimal tissue embedding is essential for smooth sectioning and ribboning. (leicabiosystems.com)
  • CHICAGO - Minimally invasive, image-guided treatment is a safe and precise method for removal of self-inflicted foreign objects from the body, according to the first report on "self-embedding disorder," or self-injury and self-inflicted foreign body insertion in adolescents. (blogspot.com)
  • 3.Our product applied to the whole operation process from tissue sectionto tissue storage inpathology laboratory. (xwlabware.com)
  • Paraffin embedded tissue is also compatible with a wide range of laboratory techniques. (ibiospecimen.com)
  • As the outbreak progressed, fluids from epidural abscesses (accumulations of pus around the spine) and tissues became the focus of laboratory testing. (cdc.gov)
  • Despite their optimism to add this type of analysis of fossil DNA to more common methods - like DNA recovered from skeletal material, mummified and frozen tissues - the researchers have no intention of raising dinosaurs. (forbes.com)
  • We successfully detected T. whipplei in tissue biopsies with a sensitivity of 83% in untreated (5/6) and 40% in treated (4/10) cases of WD. (frontiersin.org)
  • To answer some of your questions, our processor and embedding center are in separate rooms now, but not far from each other (10-12 steps). (utsouthwestern.edu)
  • By preserving the tissue in paraffin wax, it can be stored for long periods of time and can be easily transported to different laboratories for analysis. (ibiospecimen.com)
  • They can also provide recommendations for reputable laboratories or hospitals that offer paraffin embedded tissue analysis. (ibiospecimen.com)
  • 8,9 3D printing of nanocellulose hydrogels is being used in biomedical applications to support living cell growth for tissue engineering, implants, and cardiovascular devices. (cdc.gov)
  • Tissue Embedding" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Wire-mesh gloves or tongs to support the tissue can be used. (medscape.com)
  • This x-ray image illustrates 3 metal staples embedded in the hand of a teenage girl. (blogspot.com)
  • A surgical stapler for internal use is a specialized prescription device used to deliver compatible staples to internal tissues during surgery for resection, transection, and creating anastomoses. (fda.gov)
  • about 66% is found in the skeleton, 16% in the liver, 8% in the kidneys and 10% in other tissues. (who.int)
  • In Oklahoma, paraffin-embedded tissue sections are stained with various dyes to highlight different cellular components. (ibiospecimen.com)
  • The deparaffinized tissue sections are then stained with various dyes to highlight specific structures or cells of interest. (ibiospecimen.com)
  • This article describes a method for tissue preparation of radical prostatectomy that allows the production of whole-mount sections and the collection of fresh tissues for research without the use of specialized equipment. (medscape.com)
  • Briefly, paraffin-embedded tissue sections were deparaffinized, treated with 0.01?M Tris/HCl protease K (20?g/mL at 37?C) for 15?min, and then incubated with the terminal deoxynucleotidyl transferase labeling reaction combination for 60?min at 37?C. RIP1 KO not only significantly suppressed BoNT-IN-1 the tumor growth but also greatly attenuated cisplatins anticancer activity. (innovation-ecosystems-agora.com)
  • This paper describes three techniques for preparing biological tissue sections for correlation of light microscopy (LM) and scanning (SEM) and transmission (TEM) electron microscopy. (cdc.gov)
  • SE imaging of deparaffinized sections shows tissue surface, while BSE imaging reveals deeper internal structure. (cdc.gov)
  • Using zoom tomography, large areas of lung tissue embedded in wax can be scanned enabling detailed examination to locate particularly interesting areas around inflammation, blood vessels or bronchial tubes," says lead author Professor Tim Salditt from the Institute of X-ray Physics at the University of Göttingen. (newswise.com)
  • Here we show that synchrotron radiation (SR)-based microcomputed tomography (μCT) with phase contrast enhancement can resolve the collagen fibre structure of meniscus tissue, including fibre crimping and structural changes related to degeneration (Paper IV). (lu.se)
  • The antibody recognizes several low molecular weight cytokeratins, in particular cytokeratin Moll number 8 as determined by immunoblotting studies, and is highly effective for immunocytochemistry using routinely processed paraffin-embedded material. (ox.ac.uk)
  • Since X-rays penetrate deep into tissue, this enables scientists to understand the relation between the microscopic tissue structure and the larger functional architecture of an organ. (newswise.com)
  • Microscopic plastic particles have been found in the fats and lungs of two-thirds of the marine mammals in a graduate student's study of ocean microplastics, suggesting that microplastics can travel out of the digestive tract and lodge in the tissues. (aau.edu)
  • Even though MRI-based techniques may have the ability to probe the microscopic composition, other methods are needed to directly visualize the microstructure of the tissue. (lu.se)
  • This process involves taking a small piece of tissue from a patient, usually during a surgical procedure or biopsy, and placing it in a fixative solution to prevent decay and maintain its integrity. (ibiospecimen.com)
  • When a viscous substance traps a small animal, the soft tissues start to decay immediately and most DNA is lost before the entire animal is even encapsulated. (forbes.com)
  • The tissue then undergoes an incubation in lysis buffer, tissue homogenization, a 30 second sonication followed by digestion with Enzymatic Shearing Cocktail. (activemotif.com)
  • Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. (blunck-medical-books.de)
  • The researchers have yet to determine what harms embedded microplastics might cause marine mammals, but other studies have implicated plastics as possible hormone mimics and endocrine disruptors. (curbearth.com)
  • Methanol fixed and paraffin embedded (MFPE) cellblocks are an essential cytology preparation. (cytojournal.com)
  • It is commonly accepted that the volume of fixative should be 50 times greater than the size of the immersed tissue. (rndsystems.com)
  • The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. (blunck-medical-books.de)
  • Material and Method: Paraffin-embedded blocks from 30 patients with oropharynx squamous cell carcinomas, diagnosed and treated at the Oral Oncology Center were selected. (bvsalud.org)
  • Unfortunately we don't have an oven in the lab where the embedding equipment is. (utsouthwestern.edu)