Thermolysin
Trypsin
Amino Acid Sequence
Bromosuccinimide
Peptide Fragments
Chymotrypsin
Bacillus
Protease Inhibitors
Endopeptidases
Glycopeptides
Neprilysin
Enzyme that is a major constituent of kidney brush-border membranes and is also present to a lesser degree in the brain and other tissues. It preferentially catalyzes cleavage at the amino group of hydrophobic residues of the B-chain of insulin as well as opioid peptides and other biologically active peptides. The enzyme is inhibited primarily by EDTA, phosphoramidon, and thiorphan and is reactivated by zinc. Neprilysin is identical to common acute lymphoblastic leukemia antigen (CALLA Antigen), an important marker in the diagnosis of human acute lymphocytic leukemia. There is no relationship with CALLA PLANT.
Zinc
A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.
Enzyme Stability
Carboxypeptidases A
Carboxypeptidases that are primarily found the DIGESTIVE SYSTEM that catalyze the release of C-terminal amino acids. Carboxypeptidases A have little or no activity for hydrolysis of C-terminal ASPARTIC ACID; GLUTAMIC ACID; ARGININE; LYSINE; or PROLINE. This enzyme requires ZINC as a cofactor and was formerly listed as EC 3.4.2.1 and EC 3.4.12.2.
Binding Sites
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Peptide Hydrolases
Cyanogen Bromide
Thiorphan
Amino Acids
Tanning
A process of preserving animal hides by chemical treatment (using vegetable tannins, metallic sulfates, and sulfurized phenol compounds, or syntans) to make them immune to bacterial attack, and subsequent treatments with fats and greases to make them pliable. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Membrane Proteins
Cell Membrane
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Acetone
Genetic Code
Transferrin
Iron
Anions
Receptors, Transferrin
Iron Chelating Agents
Commerce
The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)
Prescription Fees
Philately
Bone Morphogenetic Proteins
Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Protein 7
Bone and Bones
Bone Morphogenetic Protein Receptors, Type I
Different regulation of the p53 core domain activities 3'-to-5' exonuclease and sequence-specific DNA binding. (1/552)
In this study we further characterized the 3'-5' exonuclease activity intrinsic to wild-type p53. We showed that this activity, like sequence-specific DNA binding, is mediated by the p53 core domain. Truncation of the C-terminal 30 amino acids of the p53 molecule enhanced the p53 exonuclease activity by at least 10-fold, indicating that this activity, like sequence-specific DNA binding, is negatively regulated by the C-terminal basic regulatory domain of p53. However, treatments which activated sequence-specific DNA binding of p53, like binding of the monoclonal antibody PAb421, which recognizes a C-terminal epitope on p53, or a higher phosphorylation status, strongly inhibited the p53 exonuclease activity. This suggests that at least on full-length p53, sequence-specific DNA binding and exonuclease activities are subject to different and seemingly opposing regulatory mechanisms. Following up the recent discovery in our laboratory that p53 recognizes and binds with high affinity to three-stranded DNA substrates mimicking early recombination intermediates (C. Dudenhoeffer, G. Rohaly, K. Will, W. Deppert, and L. Wiesmueller, Mol. Cell. Biol. 18:5332-5342), we asked whether such substrates might be degraded by the p53 exonuclease. Addition of Mg2+ ions to the binding assay indeed started the p53 exonuclease and promoted rapid degradation of the bound, but not of the unbound, substrate, indicating that specifically recognized targets can be subjected to exonucleolytic degradation by p53 under defined conditions. (+info)The cyclic structure of microcin J25, a 21-residue peptide antibiotic from Escherichia coli. (2/552)
Microcin J25 (MccJ25) is the single representative of the immunity group J of the microcin group of peptide antibiotics produced by Enterobacteriaceae. It induces bacterial filamentation in susceptible cells in a non-SOS-dependent pathway [R. A. Salomon and R. Farias (1992) J. Bacteriol. 174, 7428-7435]. MccJ25 was purified to homogeneity from the growth medium of a microcin-overproducing Escherichia coli strain by reverse-phase HPLC. Based on amino acid composition and absolute configuration determination, liquid secondary ion and electrospray mass spectrometry, extensive two-dimensional NMR, enzymatic and chemical degradations studies, the structure of MccJ25 was elucidated as a 21-residue peptide, cyclo(-Val1-Gly-Ile-Gly-Thr- Pro-Ile-Ser-Phe-Tyr-Gly-Gly-Gly-Ala-Gly-His-Val-Pro-Glu-Tyr-Phe21- ). Although MccJ25 showed high resistance to most of endoproteases, linearization by thermolysin occurred from cleavage at the Phe21-Val1 bond and led to a single peptide, MccJ25-L. While MccJ25 exhibited remarkable antibiotic activity towards Salmonella newport and several E. coli strains (minimal inhibitory concentrations ranging between 0.01 and 0.2 microgram.mL-1), the thermolysin-linearized microcin showed a dramatic decrease of the activity, indicating that the cyclic structure is essential for the MccJ25 biological properties. As MccJ25 is ribosomally synthesized as a larger peptide precursor endowed with an N-terminal extremity, the present study shows that removal of this extension and head-tail cyclization of the resulting propeptide are the only post-translational modifications involved in the maturation of MccJ25, that appears as the first cyclic microcin. (+info)Studies on the formation and stability of a complex between Streptomyces proteinaceous metalloprotease inhibitor and thermolysin. (3/552)
The effects of certain physicochemical parameters on the formation and stability of a complex between Streptomyces proteinaceous metalloprotease inhibitor (SMPI) and thermolysin were investigated. SMPI had its lowest Ki value at a pH of around 6.5 (similar to the pH dependence of the kcat/K(m) of thermolysin catalysis), reflecting the splitting mechanism of the SMPI inhibition of thermolysin. This Ki increased with an increase in pressure, and in (Ki-1) was almost linear with respect to pressure. The volume of the reaction (delta Vcomp), which is the volume change accompanying enzyme-inhibitor complex formation, was calculated as +8.1 +/- 0.3 mL.mol-1, which has a sign opposite to delta Vcomp for neutral peptide inhibitors and acyl-peptide substrates. The temperature dependence of Ki-1 gave the reaction enthalpy (delta Hcomp) and reaction entropy (delta Scomp) of the complex formation as 34.6 +/- 1.4 kJ.mol-1 and 298 +/- 5 J.mol-1.K-1, respectively. These positive reaction volumes and reaction entropies were related to the electrostatic interactions and ionic strength dependence of Ki which corresponded to the key ionic interaction during complex formation. Complex formation with SMPI stabilized thermolysin against pressure perturbation as observed by the changes in the Trp fluorescence of thermolysin with increasing pressure. Thermal stability, however, was affected very little by complex formation with SMPI. Phosphoramidon, Cbz-Phe-Gly-NH2 and Cbz-Phe also positively affected the pressure-tolerance of thermolysin, in the following order: Cbz-Gly-Phe-NH2 < Cbz-Phe << phosphoramidon. The third compound exhibited stabilizing effects comparable with those of SMPI, which suggests that the interaction between SMPI and thermolysin was localized to the reactive site. (+info)A Kazal-type trypsin inhibitor from the protochordate Ciona intestinalis. (4/552)
A trypsin inhibitor from Ciona intestinalis, present throughout the animal, was purified by ion-exchange chromatography followed by four HPLC steps. By MS the molecular mass of the native form was determined to be 6675 Da. The N-terminal amino acid sequence was determined by protein sequencing, but appeared to be partial because the theoretical molecular mass of the protein was 1101 Da too low. Thermolysin treatment gave rise to several fragments each containing a single disulphide bridge. By sequence analysis and MS intramolecular disulphide bridges could unequivocally be assigned to connect the pairs Cys4-Cys37, Cys8-Cys30 and Cys16-Cys51. The structure of the inhibitor is homologous to Kazal-type trypsin inhibitors. The inhibitor constant, KI, for trypsin inhibition was 0.05 nM whereas chymotrypsin and elastase were not inhibited. To reveal the complete sequence the cDNA encoding the trypsin inhibitor was isolated. This cDNA of 454 bp predicts a protein of 82 amino acid residues including a 20 amino acid signal peptide. Moreover, the cDNA predicts a C-terminal extension of 11 amino acids compared to the part identified by protein sequencing. The molecular mass calculated for this predicted protein is in accordance with the measured value. This C-terminal sequence is unusual for Kazal-type trypsin inhibitors and has apparently been lost early in evolution. The high degree of conservation around the active site strongly supports the importance of the Kazal-type inhibitors. (+info)Parvalbumin from rabbit muscle. Isolation and primary structure. (5/552)
A parvalbumin, with its characteristic low molecular weight (approximately 12000) acidic isoelectric point (approximately 5.5), ultraviolet spectrum (maxm 259 nm) and Ca2+-binding capacity (2 mol/mol protein) has been isolated from rabbit (Oryctolagus cuniculus) muscle. Its primary structure has been determined from a study of its tryptic peptides and of overlapping peptides generated by limited tryptic digestion and by chymotryptic and thermolytic digestions of the protein. The amino acid sequence so obtained is considered in comparison with those known for other parvalbumin and for rabbit troponin C. (+info)A three-dimensional construction of the active site (region 507-749) of human neutral endopeptidase (EC.3.4.24.11). (6/552)
A three-dimensional model of the 507-749 region of neutral endopeptidase-24.11 (NEP; E.C.3.4.24.11) was constructed integrating the results of secondary structure predictions and sequence homologies with the bacterial endopeptidase thermolysin. Additional data were extracted from the structure of two other metalloproteases, astacin and stromelysin. The resulting model accounts for the main biological properties of NEP and has been used to describe the environment close to the zinc atom defining the catalytic site. The analysis of several thiol inhibitors, complexed in the model active site, revealed the presence of a large hydrophobic pocket at the S1' subsite level. This is supported by the nature of the constitutive amino acids. The computed energies of bound inhibitors correspond with the relative affinities of the stereoisomers of benzofused macrocycle derivatives of thiorphan. The model could be used to facilitate the design of new NEP inhibitors, as illustrated in the paper. (+info)The compact conformation of fibronectin is determined by intramolecular ionic interactions. (7/552)
Fibronectin exists in a compact or extended conformation, depending upon environmental pH and salt concentration. Using recombinant fragments expressed in bacteria and baculovirus, we determined the domains responsible for producing fibronectin's compact conformation. Our velocity and equilibrium sedimentation data show that FN2-14 (a protein containing FN-III domains 2 through 14) forms dimers in low salt. Experiments with smaller fragments indicates that the compact conformation is produced by binding of FN12-14 of one subunit to FN2-3 of the other subunit in the dimer. The binding is weakened at higher salt concentrations, implying an electrostatic interaction. Furthermore, segment FN7-14+A, which contains the alternatively spliced A domain between FN11 and 12, forms dimers, whereas FN7-14 without A does not. Segment FN12-14+A also forms dimers, but the isolated A domain does not. These data imply an association of domain A with FN12-14, and the presence of A may favor an open conformation by competing with FN2-3 for binding to FN12-14. (+info)Insertion of atToc34 into the chloroplastic outer membrane is assisted by at least two proteinaceous components in the import system. (8/552)
Toc34 is a member of the outer membrane translocon complex that mediates the initial stage of protein import into chloroplasts. Toc34, like most outer membrane proteins, is synthesized in the cytosol at its mature size without a cleavable transit peptide. The majority of outer membrane proteins do not require thermolysin-sensitive components on the chloroplastic surface or ATP for their insertion into the outer membrane. However, different results have been obtained concerning the factors required for Toc34 insertion into the outer membrane. Using an Arabidopsis homologue of pea Toc34, atToc34, we show that the insertion of atToc34 was greatly reduced by thermolysin pretreatment of chloroplasts as assayed either by protease digestion or by alkaline extraction. The insertion was also dependent on the presence of ATP or GTP. A mutant of atToc34 with the GTP-binding domain deleted still required ATP for optimal insertion, indicating that ATP was used by other protein components in the import system. The ATP-supported insertion was observed even in thermolysin-pretreated chloroplasts, suggesting that the protein component responsible for ATP-stimulated insertion is a different protein from the thermolysin-sensitive component that assists atToc34 insertion. (+info)
Munin: Eksperimentell in vitro screening og molekylær dokking av forbindelser med mulig hemmende effekt på Thermolysin og...
Kinetic study of thermolysin-catalyzed synthesis of N-(benzyloxycarbonyl)-L-phenylalanyl-L-leucine ethyl ester in an ethyl...
Membrane protein shaving with thermolysin can be used to evaluate topology predictors
A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from...
RCSB PDB
- 1TRL: NMR SOLUTION STRUCTURE OF THE C-TERMINAL FRAGMENT 255-316 OF THERMOLYSIN: A DIMER FORMED BY SUBUNITS...
RCSB PDB
- 1HYT: RE-DETERMINATION AND REFINEMENT OF THE COMPLEX OF BENZYLSUCCINIC ACID WITH THERMOLYSIN AND ITS...
PDB-1fjq: THERMOLYSIN (70% ACETONE SOAKED CRYSTALS) - Yorodumi
Inouye, Kaoru | Article about Inouye, Kaoru by The Free Dictionary
KEGG ENZYME: 3.4.24.28
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3D-QSAR of Angiotensin-Converting Enzyme and Thermolysin Inhibitors: A Comparison of CoMFA Models Based on Deduced and...
ReP USP - Detalhe do registro: Thermolysin immobilized on Fe IND. 3O IND. [email protected] nanoparticle: preparation and...
2ltb - Proteopedia, life in 3D
Solubility and effective enzymatic hydrolysis of oat globulins
UV-STABILIZED PROTEIN-POLYMER COMPOSITIONS - Patent application
Aceetate [ ACEETATE, n. [See Acid.] In chimistry, a neutral salt formed by ... ] :: Search the 1828 Noah Websters...
Alpha-2-macroglobulin-like protein 1
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Joachim Jentsch : Former : Publications : Organic Chemistry : Universität Hamburg
KEGG ENZYME: 3.4.24.25
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SMART: Pfam domain Peptidase M28
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Neuroblastoma
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Brian Matthews (biochemist)
Kresge, Nicole; Simoni, Robert D.; Hill, Robert L. (2009). "Structural Studies of Thermolysin: the Work of Brian W. Matthews". ... Matthews BW, Colman PM, Jansonius JN, Titani K, Walsh KA, Neurath H (1972). "Structure of thermolysin". Nature New Biology. 238 ... He also solved early structures of the thermophilic bacterial enzyme thermolysin, the helix-turn-helix DNA-binding ...
NLN (gene)
The catalytic site is contained within a thermolysin-like region found in many metallopeptidases and located in the domain near ... Matthews BW, Weaver LH, Kester WR (December 1974). "The conformation of thermolysin". The Journal of Biological Chemistry. 249 ...
Phosphoramidon
It is an inhibitor of the enzyme thermolysin, a membrane metallo-endopeptidase inhibitor, and an endothelin converting enzyme ... "Binding between thermolysin and its specific inhibitor, phosphoramidon". Journal of Biochemistry. 95 (2): 529-34. PMID 6715312 ...
Fast parallel proteolysis
The protease thermolysin can be fully inactivated by EDTA. This feature of thermolysin makes FASTpp compatible with subsequent ... in a thermal gradient PCR cycler to different maximal temperatures in presence of the thermostable protease thermolysin (see ...
Microbial collagenase
"Circular dichroism comparative studies of two bacterial collagenases and thermolysin". Biochimica et Biophysica Acta (BBA) - ...
Talopeptin
It is a known reversible inhibitor of thermolysin and is expected to inhibit other metalloproteinases. Chemically, talopeptin ... Kitagishi, Keiko; Hiromi, Keitaro (1984). "Inhibitory spectrum of talopeptin (MKI), a specific inhibitor of thermolysin". ... "Equilibrium study on the binding between thermolysin and Streptomyces metalloprotease inhibitor, talopeptin (MKI)". Journal of ...
MMP3
Alternatively, it has been shown for thermolysin (another metalloproteinase) that the amide product can be released in its ... Hangauer DG, Monzingo AF, Matthews BW (Nov 1984). "An interactive computer graphics study of thermolysin-catalyzed peptide ... "A theoretical study of the mechanism for peptide hydrolysis by thermolysin". Journal of Biological Inorganic Chemistry. 7 (3): ...
Ecadotril
Thermolysin complexed with the inhibitor (S)-thiorphan are isomeric thiol-containing inhibitors of endopeptidase EC 24-11 (also ... The peptidase domain for members of this family also contains a bacterial member and resembles that of thermolysin the ... "Thiorphan and retro-thiorphan display equivalent interactions when bound to crystalline thermolysin". Biochemistry. 28 (4): ... predicted active site residues for members of this family and thermolysin occur in the motif HEXXH. ...
Thermostability
"Extreme stabilization of a thermolysin-like protease by an engineered disulfide bond". The Journal of Biological Chemistry. 272 ...
Dispase
2006). "A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases". Analytical Biochemistry. 352 (1 ...
Silanol
Some silanediols and silanetriols inhibit hydrolytic enzymes such as thermolysin and acetycholinesterase. Literally, silanol ...
Endopeptidase
Thermolysin - cuts before Ile, Met, Phe, Trp, Tyr, or Val, unless preceded by Pro. Sometimes cuts after Ala, Asp, His or Thr. ...
Astacin
The overall topology of this domain is shared by the archetypal zinc-endopeptidase thermolysin. Astacin protease domains also ... the type example for clan MA and the predicted active site residues for members of this family and thermolysin occur in the ... The protein fold of the peptidase domain for members of this family resembles that of thermolysin, ... molecular structure and comparison with thermolysin". Journal of Molecular Biology. 229 (4): 945-68. doi:10.1006/jmbi.1993.1098 ...
Peptide deformylase
"Substrate recognition and selectivity of peptide deformylase Similarities and differences with metzincins and thermolysin". J. ...
Ilomastat
Grobelny D, Poncz L, Galardy RE (August 1992). "Inhibition of human skin fibroblast collagenase, thermolysin, and Pseudomonas ... Examples of enzymes that ilomastat inhibit include rabbit MMP9, thermolysin, peptide deformylase, and anthrax lethal factor ...
Decellularization
Lipases, thermolysin, galactosidase, nucleases, and trypsin have all been used in the removal of cells. After a cell is lysed ...
Matrix metalloproteinase
Kester WR, Matthews BW (1977). "Crystallographic study of the binding of dipeptide inhibitors to thermolysin: implications for ...
Bacillolysin
This enzyme catalyses the following chemical reaction Similar, but not identical, to that of thermolysin This enzyme is present ... "The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral ... from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but more thermostable enzyme thermolysin". ...
Dactylysin
Joudiou C, Carvalho KM, Camarao G, Boussetta H, Cohen P (June 1993). "Characterization of the thermolysin-like cleavage of ...
Transition state analog
Thermolysin is an enzyme produced by Bacillus thermoproteolyticus that catalyses the hydrolysis of peptides containing ... Holden, Hazel; Tronrud, D. E.; Monzingo, A. F.; Weaver, L. H. (1987). "Slow-and fast-binding inhibitors of thermolysin display ... mechanism starts form the small peptide molecule and replaces the zinc binding water molecule towards Glu143 of thermolysin. ...
Thermal shift assay
However, commercially available thermolysin is dependent on calcium ions for activity and denatures itself just above 85 ... In fast parallel proteolysis the researcher adds a thermostable protease (thermolysin) and takes out samples in parallel upon ... FastPP exploits that proteins become increasingly susceptible to proteolysis when unfolded and that thermolysin cleaves at ... showed that stabilization by ligand binding could impart resistance to proteolytic digestion with thermolysin. Protection ...
Thermoase
... may refer to: Subtilisin, an enzyme Thermolysin, an enzyme This disambiguation page lists articles associated with ...
Candoxatril
Holland, D.R.; Barclay, P.L.; Danilewicz, J.C.; Matthews, B.W.; James, K. (January 1994). "Inhibition of thermolysin and ... the gluzincins a faint but significant structural relationship of the metzincins to the thermolysin-like enzymes, Zincin is the ... neutral endopeptidase 24.11 by a novel glutaramide derivative: X-ray structure determination of the thermolysin-inhibitor ...
Vibriolysin
... which distinguished this enzyme from thermolysin This thermostable enzyme is isolated from Vibrio proteolyticus. Holmquist B, ...
Oligopeptidase
... involvement of a thermolysin-like metalloendopeptidase (enkephalinase), angiotensin-converting enzyme, and other unidentified ...
Protease inhibitor (biology)
It has 102 amino acid residues with two disulphide bridges and specifically inhibits metalloproteinases such as thermolysin, ...
Protamine
It was initially produced as a mixture made by thermolysin digestion of protamine, but the actual effective peptide portion ...
Scytalidopepsin B
"Isolation and amino acid sequence of a peptide containing an epoxide-reactive residue from the thermolysin-digest of ...
Pseudolysin
... thermolysin family). Morihara K, Tsuzuki H (1975). "Pseudomonas aeruginosa elastase: affinity chromatography and some ... Insulin B chain cleavage pattern identical to that of thermolysin, but specificity differs in other respects This enzyme ...
ADAM10
... its active site is homologous to those of well studied zinc-proteases such as carboxypeptidase A and thermolysin. Therefore, it ...
Thermolysin - Wikipedia
However thermolysin is also widely used for peptide bond formation through the reverse reaction of hydrolysis. Thermolysin is ... Like all bacterial extracellular proteases thermolysin is first synthesised by the bacterium as a pre-proenzyme. Thermolysin is ... Thermolysin has a T50 value of 86.9 °C, making it the most thermo stable member of the TLP family. Studies on the contribution ... Thermolysin has a molecular weight of 34,600 Da. Its overall structure consists of two roughly spherical domains with a deep ...
Thermolysin
Sequence Similarity
- 3FLF: Thermolysin inhibition Sequence Similarity Report Page
Thermolysin 1427 3.4.24.27 , Details 100 2WHZ 1 A THERMOLYSIN THE DI-PEPTIDE IY IS BOUND TO THE PROTEIN 1427 3.4.24.27 , ... Thermolysin 1427 3.4.24.27 , Details 138 2WI0 1 A THERMOLYSIN THE DI-PEPTIDE LW IS BOUND TO THE PROTEIN 1427 3.4.24.27 , ... Thermolysin UNP residues 233-548 1427 3.4.24.27 , Details 27 5M5F 1 E Thermolysin UNP residues 233-548 1427 3.4.24.27 , Details ... Thermolysin UNP residues 233-548 1427 3.4.24.27 , Details 15 4MWP 1 E Thermolysin Mature form (UNP residues 233-548) 1427 3.4. ...
RCSB PDB
- 1PE5: Thermolysin with tricyclic inhibitor Macromolecule Annotations Page
Substrate recognition and selectivity of peptide deformylase. Similarities and differences with metzincins and thermolysin. -...
Model building of a thermolysin-like protease by mutagenesis - Research database - University of Groningen
The Advantages to Using Arg-C, Elastase, Thermolysin and Pepsin for Protein Analysis
In this article, we use four alternative proteases: Arg-C, elastase, thermolysin and pepsin, and show typical applications for ... The Elastase, Thermolysin and Pepsin Advantage. Elastase (Cat.# V1891), thermolysin (Cat.# V4001) and pepsin (Cat.# V1959) are ... Thermolysin and pepsin. Thermolysin and pepsin are distinct from other proteases because they tolerate extreme conditions: high ... Protein digestion with thermolysin is rapid because the reaction occurs at a higher temperature. However, when high temperature ...
Membrane protein shaving with thermolysin can be used to evaluate topology predictors
iv) Fewer detected peptides from thermolysin shaving originate from the transmembrane regions. Using thermolysin shaving we can ... we detect a similar number of peptides and proteins using thermolysin and trypsin; (ii) thermolysin shaving can be run at a ... At the end we suggest that the usage of thermolysin, an enzyme working at the natural pH of the cell for membrane shaving, is ... Membrane protein shaving with thermolysin can be used to evaluate topology predictors. Bendz, Maria ...
Frontiers | The selective biotin tagging and thermolysin proteolysis of chloroplast outer envelope proteins reveals information...
... based on specific tagging with biotin or proteolysis using thermolysin, a non-membrane permeable protease. To evaluate this ... based on specific tagging with biotin or proteolysis using thermolysin, a non-membrane permeable protease. To evaluate this ... away by the thermolysin treatment, whereas IEP37 and MGD1 were still detected in thermolysin-treated samples (Figures 4B-D, ... At this stage, thermolysin treatment or biotin tagging was performed as described below. Envelope, thylakoids, and stroma were ...
AID 778789 - Inhibition of Bacillus thermoproteolyticus thermolysin using FA-Fly-Leu-NH2 as substrate at pH 7.5 after 15 mins...
Magiran | comparable studies on the stability of proteases from Pseudomonas aeruginosa against thermolysin from Bacillus...
However, activity of thermolysin was abolished in all organic solvents. In the presence of DTT 1mM, the PAE activity was ... Thermolysin from Bacillus thermoproteolyticus is a highly thermostable Zn-metalloprotease and is applied in industry for ... However, thermolysin has low stability at organic solvents.In contrast, its homologous enzyme, Pseudomonas aeruginosa elastase ... In the present study, recombinant PAE was purified and compared with thermolysin in different organic solvents. For this ...
Shear stress-mediated regulation of thermolysin-like zinc metallopeptidase expression levels in vascular endothelial cells -...
Evidence by site-directed mutagenesis that arginine 203 of thermolysin and arginine 717 of neprilysin (neutral endopeptidase)...
... thermolysin (EC 3.4.24.27). One active site residue of thermolysin, Arg-203, is involved in inhibitor binding by forming ... In addition, the replacement of Asp-170 of thermolysin by Ala residue resulted in a decrease in kcat/Km of 220-fold. The ... This led, in both cases, to decreases in kcat/Km values, of 122-fold for neprilysin and 2300-fold for thermolysin, essentially ... Sequence alignment data shows that Arg-717 of neprilysin could play a similar role to Arg-203 of thermolysin. This was ...
Gentaur Molecular :Sceti K.K. \ Thermolysin \ 3504
Thermolysin \ 3504 for more molecular products just contact us ... Thermolysin (EC 3.4.24.27). [SCATT_p12040] Thermolysin. [nprS ... OV320_0165] Thermolysin (EC 3.4.24.27). [OV320_1504] Thermolysin (EC 3.4.24.27). [OV450_3101] Thermolysin (EC 3.4.24.27). [npr2 ... npr2 BT246_27540] Thermolysin (EC 3.4.24.27). [npr2 BTGOE6_18890] Thermolysin (EC 3.4.24.27). [npr2_1 BTGOE7_18030] Thermolysin ... npr2 BTI247_27420] Thermolysin (EC 3.4.24.27). [nprS SsS58_07371] Thermolysin (EC 3.4.24.27). [MME] Neprilysin (EC 3.4.24.11) ( ...
PDB-1fjq: THERMOLYSIN (70% ACETONE SOAKED CRYSTALS) - Yorodumi
THERMOLYSIN (70% ACETONE SOAKED CRYSTALS). Components. THERMOLYSIN. Details. Keywords. HYDROLASE / METALLOPROTEINASE / ORGANIC ... THERMOLYSIN. / Details. Sequence. Seq. region. Function. Str. site (34). Validation (3). Mass: 34362.305 Da / Num. of mol.: 1 ... thermolysin / metalloendopeptidase activity / extracellular region / metal ion binding. Peptidase M4/M1, CTD superfamily / ... Details: 4.6MM THERMOLYSIN, 45% DMSO, 50MM TRIS-HCL, 2.5M CSCL, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K. ...
Reversible acetonitrile-induced inactivation/activation of thermolysin<...
title = "Reversible acetonitrile-induced inactivation/activation of thermolysin",. abstract = "Thermolysin is catalytically ... Reversible acetonitrile-induced inactivation/activation of thermolysin. R.V. Ulijn, A.E.M. Janssen, B.D. Moore, P.J. Halling, S ... Reversible acetonitrile-induced inactivation/activation of thermolysin. / Ulijn, R.V.; Janssen, A.E.M.; Moore, B.D.; Halling, P ... N2 - Thermolysin is catalytically inactive in mixtures or 70-15% acetonitrile in aqueous buffer. Unexpectedly, dilution of the ...
A novel member of the thermolysin family, cloning and biochemical characterization of metalloprotease from Staphylococcus...
Colman PM, Jansonius JN, Matthews BW (1972) The structure of thermolysin: an electron density map at 2-3 Ã… resolution. J Mol ... Thermolysins constitute a family of secreted bacterial metalloproteases expressed, among others, by several pathogens. Strains ... The internal region contains the HEXXH catalytic domain that is conserved in members of the thermolysin family. Regardless of ... Buchanan JD, Corbett RJ, Roche RS (1986) The thermodynamics of calcium binding to thermolysin. Biophys Chem 23: 183-199. ...
1kjp.1 | SWISS-MODEL Template Library
Thermolysin complexed with Z-L-Glutamic acid (benzyloxycarbonyl-L-Glutamic acid) ... Thermolysin complexed with Z-L-Glutamic acid (benzyloxycarbonyl-L-Glutamic acid). Coordinates. PDB Format Method. X-RAY ... Senda, M. et al., Crystal structure analyses of thermolysin in complex with its inhibitors. To be Published Release Date. 2002- ...
SWISS-MODEL Template Library | 5m9w.1
Proteins & Enzymes, Hamster
Elastase
α-Synuclein strains target distinct brain regions and cell types | Nature Neuroscience
4 Additional thermolysin digestions of brain homogenates from inoculated TgM83 mice.. a) Immunoblots of detergent-insoluble α- ... a) Thermolysin digestion of brain extracts from the three human synucleinopathy samples inoculated into TgM83 mice. α-Syn was ... with or without digestion with thermolysin (TL). Brain homogenates from asymptomatic TgM83 mice from the second passage of PBS ...
Compound Report Card
Membrane transporters in the relict plastid of malaria parasites | PNAS
25 μg of thermolysin per mg of protein (+ thermolysin), (iii) 10 mM EDTA and 25 μg of thermolysin per mg of protein (inhibited ... thermolysin), and (iv) 0.1% Triton X-100 and 25 μg of thermolysin per mg of protein (permeabilized organelles + thermolysin). ... Intact apicoplasts were incubated with thermolysin, then examined by Western blotting (28). Thermolysin treatment of intact ... D) Thermolysin digestion of free, intact apicoplasts from parasites transfected with an N-terminally tagged copy of PfoTPT (HA- ...
Heat-stability of a proteinase from psychrotrophic Pseudomonas fluorescens P38, chymotrypsin and thermolysin<...
Owusu R, Doble C. Heat-stability of a proteinase from psychrotrophic Pseudomonas fluorescens P38, chymotrypsin and thermolysin ... Heat-stability of a proteinase from psychrotrophic Pseudomonas fluorescens P38, chymotrypsin and thermolysin. Richard Owusu, C ... Heat-stability of a proteinase from psychrotrophic Pseudomonas fluorescens P38, chymotrypsin and thermolysin. / Owusu, Richard ... title = "Heat-stability of a proteinase from psychrotrophic Pseudomonas fluorescens P38, chymotrypsin and thermolysin", ...
Zeitschrift für Naturforschung B
Patent US4789732 - Bone morphogenetic protein composition - Google Patents
Molecules | Free Full-Text | Anti-Inflammatory and Antioxidant Properties of Peptides Released from β-Lactoglobulin by High...
Thermolysin. Protease from Bacillus thermoproteolyticus. 14 U/mg. pH 7; 50 °C. ... The BLG was hydrolysed by neutrase (Neu), alcalase (Alc), savinase (Sav), elastase (Ela), thermolysin (Ther), and trypsin (Try ... Thermolysin (Ther), and Savinase (Sav). The statistical analysis of the results was performed by two-way ANOVA followed by a ... Thermolysin (Ther), and Savinase (Sav). The statistical analysis of the results was performed by two-way ANOVA followed by a ...
Thermolysin in human cultured keratinocyte isolation | Braz. j. biol;67(1): 105-109, Feb. 2007. ilus | LILACS
Thermolysin in human cultured keratinocyte isolation Gragnani, A; Sobral, C. S; Ferreira, L. M. *Gragnani, A; Federal ... Full text: Available Index: LILACS (Americas) Main subject: Thermolysin / Trypsin / Keratinocytes / Cell Separation / Colony- ... Full text: Available Index: LILACS (Americas) Main subject: Thermolysin / Trypsin / Keratinocytes / Cell Separation / Colony- ... To compare the action of trypsin and thermolysin in the keratinocyte isolation using newborn foreskin. METHODS:. This method ...
BacillusProteaseProteasesChymotrypsinEnzyme thermolysinTrypsin24.27BacterialEnzymesProteinsPeptidesInhibitorsStudies of thermolysinActivation of thermolysinMetalloproteasesRecombinantHydrolysisOrganic solventsInactivationZincProteinAmino acidMetalloendopeptidaseInhibitorSequenceAqueousRESIDUESSynthesisStructuralPeptideMoleculeCalciumMolecularActivityFamilyStableYieldTopologyEssentiallyReactionMethod
Bacillus6
- Thermolysin (EC 3.4.24.27, Bacillus thermoproteolyticus neutral proteinase, thermoase, thermoase Y10, TLN) is a thermostable neutral metalloproteinase enzyme produced by the Gram-positive bacteria Bacillus thermoproteolyticus. (wikipedia.org)
- Thermolysin is the most stable member of a family of metalloproteinases produced by various Bacillus species. (wikipedia.org)
- Changing these amino acids to threonine (T) and alanine (A) respectively in a less stable thermolysin-like proteinase produced by Bacillus stearothermophillus (TLP-ste), results in individual reductions in stability of 7 °C (F63→T) and 6.3 °C (P69→A) and when combined a reduction in stability of 12.3 °C. In the synthesis of aspartame, less bitter-tasting byproduct is produced when the reaction is catalyzed by thermolysin. (wikipedia.org)
- 1976). "Role of calcium ions in the thermostability of thermolysin and Bacillus subtilis var. (wikipedia.org)
- Thermolysin from Bacillus thermoproteolyticus is a highly thermostable Zn-metalloprotease and is applied in industry for peptide synthesis, a reaction essentially performs in organic media. (magiran.com)
- De eksperimentelle forsøkene gav Km verdi for Bradykinin lignende substrat for Thermolysin fra Bacillus thermoproteolyticus eubakterie på 17.7 +/- 4.3 μM og Pseudolysin fra Pseudomonas aeruginosa på 7.4 +/- 1.3 μM. (uit.no)
Protease6
- We sought a method providing information at the surface of the outer envelope membrane (OEM), based on specific tagging with biotin or proteolysis using thermolysin, a non-membrane permeable protease. (frontiersin.org)
- However, thermolysin has low stability at organic solvents.In contrast, its homologous enzyme, Pseudomonas aeruginosa elastase (PAE) is an organic solvent stable protease. (magiran.com)
- 8 of these 5 mL samples were prepared, and each of the four proteases (proteinase K, trypsin, chymotrypsin, and thermolysin) were added in the amounts specified in their respective reaction protocols to achieve final protease concentrations of 1 and 100 nM. (openwetware.org)
- The serine protease ALP-901 produced the highest yield after 48 hours reaction time (53 %), while the reaction with thermolysin achieved the overall highest yield (63 %) after 6 hours, with only monoesters synthesised. (aau.dk)
- The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or. (patents.com)
- HA/protease belongs to the thermolysin family of bacterial zinc metalloproteases ( 36 ). (asm.org)
Proteases4
- Like all bacterial extracellular proteases thermolysin is first synthesised by the bacterium as a pre-proenzyme. (wikipedia.org)
- Based on the assumption that leptospiral proteases play a crucial role during infection, the aim of this work was to obtain a functional recombinant thermolysin for future studies on the role of these metalloproteases on leptospiral infection. (butantan.gov.br)
- Beynon, R.J. & Beaumont, A. (1998) M4 proteases: thermolysins. (liverpool.ac.uk)
- The enzymes reviewed include collagenase, trypsin, clostripain, and the neutral proteases dispase and thermolysin. (mendeley.com)
Chymotrypsin1
- The heat-stabilities of proteinases from a psychrotrophic Psuedomonas fluorescens strain P38 (P38 proteinase), chymotypsin, succinyl-chymotrypsin and thermolysin are compared on the basis of their respective thermoinactivation-rate constants and Arrhenius plots (40-145°C). The Arrhenius plots for P38 proteinase and succinyl-chymotrypsin showed an inversion at 80-90°C characteristic of enzymes showing low-temperature inactivation (LTI). (ulster.ac.uk)
Enzyme thermolysin2
- He also solved early structures of the thermophilic bacterial enzyme thermolysin, the helix-turn-helix DNA-binding transcription factor lambda Cro repressor, and the light-antenna bacteriochlorophyll protein. (wikipedia.org)
- The enzyme thermolysin catalyses the break up of peptides - components of proteins - in bacterial cells. (newscientist.com)
Trypsin4
- To compare the action of trypsin and thermolysin in the keratinocyte isolation using newborn foreskin . (bvsalud.org)
- The number of colonies in the thermolysin group was significantly greater than in the trypsin group. (bvsalud.org)
- B) Analysis of conformational changes due to thermolysin and trypsin. (asm.org)
- srr7 virions were incubated with either 40 nM soMHVR (+) or BSA (−) at 37°C for 30 min, and then samples were digested with 15 μg of thermolysin/ml or 100 μg of trypsin/ml at 4°C for 30 min. (asm.org)
24.271
- Although no atomic structure is available for this enzyme, site-directed mutagenesis studies have shown that its active site resembles closely that of the bacterial zinc-endopeptidase, thermolysin (EC 3.4.24.27). (inserm.fr)
Bacterial3
- Thermolysins constitute a family of secreted bacterial metalloproteases expressed, among others, by several pathogens. (edu.pl)
- A volumetric yield of 46 mg thermolysin/L of bacterial culture and a yield of near 100% in relation to the total thermolysin present in TL-IBs were obtained. (butantan.gov.br)
- Bacterial Thermolysin (TLN). (biolegend.com)
Enzymes5
- These enzymes are also termed 'neutral' proteinases or thermolysin -like proteinases (TLPs). (wikipedia.org)
- The results, coupled with a molecular modeling study, suggest that Arg-717 of neprilysin corresponds to Arg-203 of thermolysin and that in both enzymes a hydrogen bond network exists, involving His-142, Asp-170, and Arg-203 in thermolysin and His-583, Asp-650, and Arg-717 in neprilysin, which is crucial for hydrolytic activity. (inserm.fr)
- Enzymes from the thermolysin family are crucial factors in the pathogenesis of several diseases caused by bacteria and are potential targets for therapeutic interventions. (butantan.gov.br)
- Enzymes that may be species variants of thermolysin are reported from Micrococcus caseolyticus [4] and Aspergillus oryzae [5]. (genome.jp)
- S. Blumberg, Z. Vogel, and M. Altstein, Inhibition of enkephalin-degrading enzymes from rat brain and of thermolysin by amino acid hydroxamates, Life Sci. (springer.com)
Proteins7
- In contrast to many proteins that undergo conformational changes upon heating and denaturation, thermolysin does not undergo any major conformational changes until at least 70 °C. The thermal stability of members of the TLP family is measured in terms of a T50 temperature. (wikipedia.org)
- Preventing this calcium from originally binding to the molecule by mutation of its binding site, reduced thermolysin stability by 7 °C. However, while calcium binding makes a significant contribution to stabilising thermolysin, more crucial to stability is a small cluster of N-terminal domain amino acids located at the proteins surface. (wikipedia.org)
- Thermolysin encoded by the gene LIC13322 of the causative agent of leptospirosis, Leptospira interrogans, was shown to cleave proteins from the Complement System. (butantan.gov.br)
- Major proteins of these globules, and those treated with thermolysin after isolation, were identical. (springer.com)
- However, the purified proteins were sensitive to thermolysin, indicating that domains of them are normally hidden in the globule matrix. (springer.com)
- These results indicate that the five thermolysin-resistant proteins (apparent M r values: 56, 52, 32, 29, 27 kDa) are close to the surface and might be crucial for globule stabilization, whereas the thermolysin-accessible proteins are probably involved in globule/globule interactions and/or globule/eyespot-membrane interactions. (springer.com)
- An analysis of successful SAD phasing on three proteins, lysozyme, glucose isomerase and thermolysin based on the signal of weak anomalous scatterers such as sulfur atom and chloride ion have been carried out. (scirp.org)
Peptides3
- iv) Fewer detected peptides from thermolysin shaving originate from the transmembrane regions. (diva-portal.org)
- Novel Angiotensin I-Converting Enzyme Inhibitory Peptides Found in a Thermolysin-Treated Elastin with Antihypertensive Activity. (peptide.com)
- After digestion by thermolysin and pronase, cofactor peptides were purified by HPLC and sequenced. (sigmaaldrich.com)
Inhibitors5
- Crystal structure analyses of thermolysin in complex with its inhibitors. (expasy.org)
- The ability of comparative molecular field analysis (CoMFA), a three-dimensional, quantitative structure-activity relationship (3-D QSAR) paradigm, to predict the activity of inhibitors of angiotensin-converting enzyme (ACE) and thermolysin was examined. (wustl.edu)
- 1987, 1, 3-16) were comparable to those derived for the thermolysin inhibitors using alignment rules defined by crystallographic data. (wustl.edu)
- Experimentally derived alignment rules based on known structures of three-dimensional complexes produced predictive correlations for thermolysin inhibitors comparable, but not superior, to the correlations for ACE inhibitors based on alignment rules which were computationally deduced. (wustl.edu)
- Methyl, Ethyl, Propyl, Butyl: Futile But Not for Water, as the Correlation of Structure and Thermodynamic Signature Shows in a Congeneric Series of Thermolysin Inhibitors. (cathdb.info)
Studies of thermolysin1
- Circular dichroism and fluorescence studies of thermolysin in the same solvent mixtures reveal discontinuous changes in the overall secondary and tertiary protein structure that correlate well with the reversible differences in catalytic activity. (strath.ac.uk)
Activation of thermolysin2
- Price, N.C. / Reversible acetonitrile-induced inactivation/activation of thermolysin . (strath.ac.uk)
- Inouye, K , Lee, SB & Tonomura, B 1996, ' Effect of amino acid residues at the cleavable site of substrates on the remarkable activation of thermolysin by salts ', Biochemical Journal , vol. 315, no. 1, pp. 133-138. (elsevier.com)
Metalloproteases1
- Several species including Vibrio cholerae and V. vulnificus are known to produce thermolysin-like metalloproteases termed vibriolysin. (frontiersin.org)
Recombinant2
- In the present study, recombinant PAE was purified and compared with thermolysin in different organic solvents. (magiran.com)
- However, the production of this recombinant protein using traditional refolding processes with high levels of denaturing reagents for thermolysin inclusion bodies (TL-IBs) solubilization results in poor recovery and low proteolytic activity probably due to improper refolding of the protein. (butantan.gov.br)
Hydrolysis5
- Thermolysin specifically catalyzes the hydrolysis of peptide bonds containing hydrophobic amino acids. (wikipedia.org)
- However thermolysin is also widely used for peptide bond formation through the reverse reaction of hydrolysis. (wikipedia.org)
- Based on the numerous homologies that deformylase displays with thermolysin and metzincins, a mechanism of enzyme:substrate recognition and hydrolysis could finally be proposed. (nih.gov)
- Evidence by site-directed mutagenesis that arginine 203 of thermolysin and arginine 717 of neprilysin (neutral endopeptidase) play equivalent critical roles in substrate hydrolysis and inhibitor binding. (inserm.fr)
- The activity of thermolysin in the hydrolysis of N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide and N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester is remarkably enhanced in the presence of high concentrations (1-5 M) of neutral salts. (elsevier.com)
Organic solvents1
- However, activity of thermolysin was abolished in all organic solvents. (magiran.com)
Inactivation1
- Studies on the contribution of calcium to thermolysin stability have shown that upon thermal inactivation a single calcium ion is released from the molecule. (wikipedia.org)
Zinc5
- Dette er en interessant observasjon siden man antar at spesifikke hemmere må binde til bindingsseter som ikke inkulderer det katalytiske Zinc atomet, siden katalytisk Zinc finnes i alle zinc metalloproteaser. (uit.no)
- The predictive ability of the ACE model for a series of molecules not included in the training set was improved by the addition of a zinc indicator variable which explicitly defined the nature of the zinc-ligand interaction, an effect not observed within the thermolysin series. (wustl.edu)
- Thermolysin: a zinc metalloenzyme. (genome.jp)
- W. Structural basis of the action of thermolysin and related zinc peptidases. (genome.jp)
- The overall topology of this domain is shared by the archetypal zinc-endopeptidase thermolysin. (ebi.ac.uk)
Protein2
- Thermolysin showed a biphasic log k versus 1/T plot consistent with the two-state model for protein and enzyme denaturation. (ulster.ac.uk)
- This group of metallopeptidases belong to the MEROPS peptidase family M12, subfamily M12A (astacin family, clan MA(M)). The protein fold of the peptidase domain for members of this family resembles that of thermolysin, the type example for clan MA and the predicted active site residues for members of this family and thermolysin occur in the motif HEXXH [ PMID: 7674922 ]. (ebi.ac.uk)
Amino acid2
- In particular a phenylalanine (F) at amino acid position 63 and a proline (P) at amino acid position 69 contribute significantly to thermolysin stability. (wikipedia.org)
- Amino-acid sequence of thermolysin. (genome.jp)
Metalloendopeptidase1
- Human NEP can be classified as a thermolysin-type metalloendopeptidase (2,10,11). (springer.com)
Inhibitor2
- One active site residue of thermolysin, Arg-203, is involved in inhibitor binding by forming hydrogen bonds with the carbonyl group of a residue in the P1 position and also participates in a hydrogen bond network involving Asp-170. (inserm.fr)
- Experimental MAD phased structure of thermolysin in complex with inhibitor JC65. (expasy.org)
Sequence1
- Sequence alignment data shows that Arg-717 of neprilysin could play a similar role to Arg-203 of thermolysin. (inserm.fr)
Aqueous2
- Thermolysin is catalytically inactive in mixtures or 70-15% acetonitrile in aqueous buffer. (strath.ac.uk)
- The kinetics of the thermolysin-catalyzed synthesis of N-(benzyloxycarbonyl)-L-phenylalanyl-L-leucine ethyl ester (Z-Phe-LeuOEt) from N-(benzyloxycarbonyl)-L-phenylalanine (Z-Phe) and L-leucine ethyl ester (LeuOEt) in an ethyl acetate saturated aqueous system in a batch operation were studied. (elsevier.com)
RESIDUES1
- This was investigated by site-directed mutagenesis with Arg-203 of thermolysin and Arg-717 of neprilysin being replaced by methionine residues. (inserm.fr)
Synthesis1
- Within the pH and temperature range examined, the k cat value for the synthesis of Z-Phe-LeuOEt reached a maximum at pH 7.0 and 45°C, whereas the affinity between Z-Phe and thermolysin reached a maximum at pH 6.0 and 40°C. The inhibitory effect of Z-Phe on the condensation reaction decreased as the pH and temperature decreased. (elsevier.com)
Structural1
- Crystal structures of alpha-mercaptoacyldipeptides in the thermolysin active site: structural parameters for a Zn monodentation or bidentation in metalloendopeptidases. (cathdb.info)
Peptide2
- Thermolysin is synthesized as a pre-proenzyme consisting of a signal peptide 28 amino acids long, a pro-peptide 204 amino acids long and the mature enzyme itself 316 amino acids in length. (wikipedia.org)
- Human FXI (hFXI) activity is measured by its ability to cleave a fluorogenic peptide substrate Boc-IEGR-AMC after hFXI is activated by thermolysin. (biolegend.com)
Molecule1
- SEC-purified thermolysin suffers fragmentation, likely due to autoproteolysis and presents proteolytic activity against complement C3 a-chain, possibly by a generation of a C3b-like molecule. (butantan.gov.br)
Calcium1
- Buchanan JD, Corbett RJ, Roche RS (1986) The thermodynamics of calcium binding to thermolysin. (edu.pl)
Molecular1
- Thermolysin has a molecular weight of 34,600 Da. (wikipedia.org)
Activity2
- The proteolytic activity of thermolysin against C3 was time and dose-dependent. (butantan.gov.br)
- Thermolysin-activated human FXI hydrolyzes a fluorogenic substrate, Boc-IEGR-AMC, with a specific activity value greater than 200 pmol/µg/min. (biolegend.com)
Family2
- Thermolysin has a T50 value of 86.9 °C, making it the most thermo stable member of the TLP family. (wikipedia.org)
- The internal region contains the HEXXH catalytic domain that is conserved in members of the thermolysin family. (edu.pl)
Stable1
- These globules were not stable and easily fused, whereas thermolysin-treated globules were relatively stable. (springer.com)
Yield1
- Thermolysin was added to improve the yield of epithelial cells , while endothelin-3 was added to stimulate their growth . (bvsalud.org)
Topology1
- Using thermolysin shaving we can also provide a clear separation between the best and the less accurate topology predictors, indicating that using data from shaving can provide valuable information when developing new topology predictors. (diva-portal.org)
Essentially1
- This led, in both cases, to decreases in kcat/Km values, of 122-fold for neprilysin and 2300-fold for thermolysin, essentially due to changes in kcat. (inserm.fr)
Reaction2
- The highest concentration of 2- O -lauroyl sucrose observed was 23.7 mM after 24 hours in the thermolysin-catalysed reaction. (aau.dk)
- The concentration of sucrose laurate was observed to decline with reaction times above 6 hours in the reaction with thermolysin due to oligoester formation, and declining concentration with increasing reaction time was also observed for some specific sucrose laurate regioisomers in the reactions with other enzyme formulations. (aau.dk)
Method2
- This method used thermolysin as it selectively digests the dermo-epidermal junction. (bvsalud.org)
- Differentiated human intestinal epithelial cells , with the expression of surface markers (cytokeratins 8, 18 and mouse intestinal mucosa -1/39) and secretion of cytokines (sucrase-isomaltase, aminopeptidase N and dipeptidylpeptidase IV), may be cultured by the thermolysin and endothelin-3 method and maintained for at least 20 passages. (bvsalud.org)