A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
Neoplasms located in the blood and blood-forming tissue (the bone marrow and lymphatic tissue). The commonest forms are the various types of LEUKEMIA, of LYMPHOMA, and of the progressive, life-threatening forms of the MYELODYSPLASTIC SYNDROMES.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed)
Established cell cultures that have the potential to propagate indefinitely.
A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The parent alcohol of the tumor promoting compounds from CROTON OIL (Croton tiglium).
Mammalian pigment cells that produce MELANINS, pigments found mainly in the EPIDERMIS, but also in the eyes and the hair, by a process called melanogenesis. Coloration can be altered by the number of melanocytes or the amount of pigment produced and stored in the organelles called MELANOSOMES. The large non-mammalian melanin-containing cells are called MELANOPHORES.
The two longitudinal ridges along the PRIMITIVE STREAK appearing near the end of GASTRULATION during development of nervous system (NEURULATION). The ridges are formed by folding of NEURAL PLATE. Between the ridges is a neural groove which deepens as the fold become elevated. When the folds meet at midline, the groove becomes a closed tube, the NEURAL TUBE.
Insoluble polymers of TYROSINE derivatives found in and causing darkness in skin (SKIN PIGMENTATION), hair, and feathers providing protection against SUNBURN induced by SUNLIGHT. CAROTENES contribute yellow and red coloration.
An enzyme of the oxidoreductase class that catalyzes the reaction between L-tyrosine, L-dopa, and oxygen to yield L-dopa, dopaquinone, and water. It is a copper protein that acts also on catechols, catalyzing some of the same reactions as CATECHOL OXIDASE. EC 1.14.18.1.
A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445)
A genus of BIRDS in the family Phasianidae, order GALLIFORMES, containing the common European and other Old World QUAIL.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Tumors or cancer of the SKIN.
Neoplasms composed of glandular tissue, an aggregation of epithelial cells that elaborate secretions, and of any type of epithelium itself. The concept does not refer to neoplasms located in the various glands or in epithelial tissue.
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
A circumscribed benign epithelial tumor projecting from the surrounding surface; more precisely, a benign epithelial neoplasm consisting of villous or arborescent outgrowths of fibrovascular stroma covered by neoplastic cells. (Stedman, 25th ed)
Tumors or cancer of the PROSTATE.
Compounds that interact with ANDROGEN RECEPTORS in target tissues to bring about the effects similar to those of TESTOSTERONE. Depending on the target tissues, androgenic effects can be on SEX DIFFERENTIATION; male reproductive organs, SPERMATOGENESIS; secondary male SEX CHARACTERISTICS; LIBIDO; development of muscle mass, strength, and power.
An indolocarbazole that is a potent PROTEIN KINASE C inhibitor which enhances cAMP-mediated responses in human neuroblastoma cells. (Biochem Biophys Res Commun 1995;214(3):1114-20)
Tumor-promoting compounds obtained from CROTON OIL (Croton tiglium). Some of these are used in cell biological experiments as activators of protein kinase C.
Proteins, generally found in the CYTOPLASM, that specifically bind ANDROGENS and mediate their cellular actions. The complex of the androgen and receptor migrates to the CELL NUCLEUS where it induces transcription of specific segments of DNA.

The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (1/10860)

Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha-stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha-inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness.  (+info)

Activation of IkappaB kinase beta by protein kinase C isoforms. (2/10860)

The atypical protein kinase C (PKC) isotypes (lambda/iotaPKC and zetaPKC) have been shown to be critically involved in important cell functions such as proliferation and survival. Previous studies have demonstrated that the atypical PKCs are stimulated by tumor necrosis factor alpha (TNF-alpha) and are required for the activation of NF-kappaB by this cytokine through a mechanism that most probably involves the phosphorylation of IkappaB. The inability of these PKC isotypes to directly phosphorylate IkappaB led to the hypothesis that zetaPKC may use a putative IkappaB kinase to functionally inactivate IkappaB. Recently several groups have molecularly characterized and cloned two IkappaB kinases (IKKalpha and IKKbeta) which phosphorylate the residues in the IkappaB molecule that serve to target it for ubiquitination and degradation. In this study we have addressed the possibility that different PKCs may control NF-kappaB through the activation of the IKKs. We report here that alphaPKC as well as the atypical PKCs bind to the IKKs in vitro and in vivo. In addition, overexpression of zetaPKC positively modulates IKKbeta activity but not that of IKKalpha, whereas the transfection of a zetaPKC dominant negative mutant severely impairs the activation of IKKbeta but not IKKalpha in TNF-alpha-stimulated cells. We also show that cell stimulation with phorbol 12-myristate 13-acetate activates IKKbeta, which is entirely dependent on the activity of alphaPKC but not that of the atypical isoforms. In contrast, the inhibition of alphaPKC does not affect the activation of IKKbeta by TNF-alpha. Interestingly, recombinant active zetaPKC and alphaPKC are able to stimulate in vitro the activity of IKKbeta but not that of IKKalpha. In addition, evidence is presented here that recombinant zetaPKC directly phosphorylates IKKbeta in vitro, involving Ser177 and Ser181. Collectively, these results demonstrate a critical role for the PKC isoforms in the NF-kappaB pathway at the level of IKKbeta activation and IkappaB degradation.  (+info)

Enhanced adhesion of Pasteurella multocida to cultured turkey peripheral blood monocytes. (3/10860)

Capsular hyaluronic acid (HA) mediates adhesion of serogroup A strains of Pasteurella multocida to elicited turkey air sac macrophages (TASM). In contrast, freshly isolated turkey peripheral blood monocytes (TPBM) do not bind serogroup A strains. Following culture of TPBM for 6 days in chamber slides, adhesion of the bacteria to TPBM increased gradually. Incubation in chamber slides coated with entactin-collagen IV-laminin (ECL) attachment matrix or exposure to phorbol myristate acetate (PMA) further enhanced the adhesion of P. multocida to TPBM. Addition of HA, but not Arg-Gly-Asp peptide, to TPBM culture inhibited bacterial adherence similarly to the inhibition previously reported for TASM. Exposure of TPBM to monoclonal antibody directed against HA-binding cell surface proteoglycan (CD44) decreased binding of P. multocida. Collectively, these findings indicate that P. multocida adhesion to TPBM is mediated by capsular HA and can be increased by culture on ECL attachment matrix or PMA exposure. Additionally, the findings suggest that the capsular mucopolysaccharide of serogroup A strains of P. multocida recognizes an isoform of CD44 expressed on cultured TPBM.  (+info)

Modulation of basal intracellular calcium by inverse agonists and phorbol myristate acetate in rat-1 fibroblasts stably expressing alpha1d-adrenoceptors. (4/10860)

In rat-1 fibroblasts stably expressing alpha1d-adrenoceptors BMY 7378, phentolamine, chloroethylclonidine and 5-methyl urapidil decreased basal [Ca2+]i. WB 4101 induced a very small effect on this parameter but when added before the other antagonists it blocked their effect. All these agents inhibited the action of norepinephrine. Phorbol myristate acetate also blocked the effect of norepinephrine and decreased basal [Ca2+]i. Staurosporine inhibited these effects of the phorbol ester. Our results suggest that: (1) alpha1d-adrenoceptors exhibit spontaneous ligand-independent activity, (2) BMY 7378, phentolamine, chloroethylclonidine and 5-methyl urapidil act as inverse agonists and (3) protein kinase C activation blocks spontaneous and agonist-stimulated alpha1d-adrenoceptor activity.  (+info)

Down-regulation of oxytocin-induced cyclooxygenase-2 and prostaglandin F synthase expression by interferon-tau in bovine endometrial cells. (5/10860)

Oxytocin (OT) is responsible for the episodic release of luteolytic prostaglandin (PG) F2alpha from the uterus in ruminants. The attenuation of OT-stimulated uterine PGF2alpha secretion by interferon-tau (IFN-tau) is essential for prevention of luteolysis during pregnancy in cows. To better understand the mechanisms involved, the effect of recombinant bovine IFN-tau (rbIFN-tau) on OT-induced PG production and cyclooxygenase-2 (COX-2) and PGF synthase (PGFS) expression in cultured endometrial epithelial cells was investigated. Cells were obtained from cows at Days 1-3 of the estrous cycle and cultured to confluence in RPMI medium supplemented with 5% steroid-free fetal calf serum. The cells were then incubated in the presence or absence of either 100 ng/ml OT or OT+100 ng/ml rbIFN-tau for 3, 6, 12, and 24 h. OT significantly increased PGF2alpha and PGE2 secretion at all time points (p < 0.01), while rbIFN-tau inhibited the OT-induced PG production and reduced OT receptor binding in a time-dependent manner. OT increased the steady-state level of COX-2 mRNA, measured by Northern blot, which was maximal at 3 h (9-fold increase) and then decreased with time (p < 0.01). OT also caused an increase in COX-2 protein, which peaked at 12 h (11-fold increase), as measured by Western blot. Addition of rbIFN-tau suppressed the induction of COX-2 mRNA (89%, p < 0.01) and COX-2 protein (50%, p < 0.01) by OT. OT also increased PGFS mRNA, and this stimulation was attenuated by rbIFN-tau (p < 0.01). To ensure that the decrease in COX-2 was not solely due to down-regulation of the OT receptor, cells were stimulated with a phorbol ester (phorbol 12-myristate 13-acetate; PMA) in the presence and absence of rbIFN-tau. The results showed that rbIFN-tau also decreased PMA-stimulated PG production and COX-2 protein. It can be concluded that rbIFN-tau inhibition of OT-stimulated PG production is due to down-regulation of OT receptor, COX-2, and PGFS.  (+info)

Acetyl-CoA:1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase is directly activated by p38 kinase. (6/10860)

Acetyl-CoA:1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase, along with phospholipase A2, is a key regulator of platelet-activating factor biosynthesis via the remodeling pathway. We have now obtained evidence in human neutrophils indicating that this enzyme is regulated by a specific member of the mitogen-activated protein kinases, namely the p38 kinase. We earlier demonstrated that tumor necrosis factor-alpha (TNF-alpha) as well as N-formyl-methionyl-leucyl-phenylalanine treatment leads to increased phosphorylation and activation of p38 kinase in human neutrophils. Strikingly, in the present study these stimuli increased the catalytic activity of acetyltransferase up to 3-fold, whereas 4-phorbol 12-myristate 13-acetate, which activates the extracellular-regulated kinases (ERKs) but not p38 kinase, had no effect. Furthermore, a selective inhibitor of p38 kinase, SB 203580, was able to abolish the TNF-alpha- and N-formyl-methionyl-leucyl-phenylalanine-induced activation of acetyltransferase. The same effect was not observed in the presence of an inhibitor that blocked ERK activation (PD 98059). Complementing the findings in intact cells, we have shown that recombinant, activated p38 kinase added to microsomes in the presence of Mg2+ and ATP increased acetyltransferase activity to the same degree as in microsomes obtained from TNF-alpha-stimulated cells. No activation of acetyltransferase occurred upon treatment of microsomes with either recombinant, activated ERK-1 or ERK-2. Finally, the increases in acetyltransferase activity induced by TNF-alpha could be ablated by treating the microsomes with alkaline phosphatase. Thus acetyltransferase appears to be a downstream target for p38 kinase but not ERKs. These data from whole cells as well as cell-free systems fit a model wherein stimulus-induced acetyltransferase activation is mediated by a phosphorylation event catalyzed directly by p38 kinase.  (+info)

Molecular mechanisms of proliferation in endometrial tumour cells. (7/10860)

The human endometrium normally undergoes a cyclic proliferation process followed by differentiation under the influence of ovarian steroids and locally produced growth and differentiation factors. Understanding of the molecular mechanisms involved in controlling these processes is of great interest, since imbalances between proliferation- and differentiation-promoting signals can have pathophysiological consequences ranging from infertility to endometrial hyperplasia and tumour formation. The present work reviews aspects of the role played by oncogenes and ovarian steroid receptors in modulating proliferation of endometrial tumour cells. The expression pattern and possible roles of protein kinase C (PKC) subunits are discussed in the context of response-specificity of endometrial tumour cells to tumour-promoting agents such as 12-O-tetradecanoyl-phorbol acetate (TPA) and possible implications for anti-tumour therapy.  (+info)

Expression of dominant negative Erk2 inhibits AP-1 transactivation and neoplastic transformation. (8/10860)

The mitogen activated protein (MAP) kinases or extracellular signal-regulated kinases (Erks) are activated in response to Ras expression or exposure to tumor promoters or to growth factors, and have been implicated in AP-1 transactivation in some models. We have shown that tumor promoter induced activation of the transcription factor AP-1 is required for induced neoplastic transformation in the Balb/C JB6 cell model. Jun and Fos family protein levels have been found not to be limiting for AP-1 response. The present study asks whether activation of Erks1 and 2 is required for AP-1 transactivation and transformation of JB6 cells and whether Erks might be targeted for cancer prevention. Expression of either of two different dominant negative kinase inactive Erk2 mutants in transformation sensitive (P+) JB6 cells substantially inhibited the tumor promoter induced activation of Erks1 and 2 and of AP-1 measured by a collagenase-luciferase reporter. Multiple mutant Erk2 expressing clonal lines were also rendered non-responsive to induced neoplastic transformation. These observations, together with our recent finding attributing AP-1 non-responsiveness to Erk deficiency in a clonal line of transformation resistant (P-) cells, argue for a requirement for Erks1 and/or 2 activation in AP-1 transactivation in the mouse JB6 neoplastic progression model, and suggest the utility of Erks as a prevention target.  (+info)

TY - JOUR. T1 - Effect of phorbol myristate acetate-induced lung injury on airway blood flow. AU - Barman, Scott A. AU - Ardell, J. L.. AU - Taylor, A. E.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - The effects of phorbol myristate acetate (PMA) induced lung injury on the pulmonary and systemic blood flow contributions to the trachea and main bronchi (upper airways) were assessed in anesthetized dogs by injecting 15 μm radiolabeled microspheres into the right and left heart, respectively. Upper airway blood flow was studied in lungs given the following treatments: (1) PMA; (2) PMA in lungs pretreated with the thromboxane synthetase inhibitor OKY-046, and (3) PMA in lungs pretreated with the antioxidant catalase. After microsphere injections, the tracheal cartilage, tracheal muscle-mucosa, and main bronchi were excised. The results of this study indicate that under normal conditions, tracheal mucosa [33-52 ml·min-·(100 g)-1] and tracheal cartilage [18-27 ml·min-1·(100 g)-1] blood flow is primarily ...
We isolated a group of genes that are rapidly and transiently induced in 3T3 cells by tetradecanoyl phorbol acetate (TPA). These genes are called TIS genes (for TPA-inducible sequences). Epidermal growth factor (EGF), fibroblast growth factor (FGF), and TPA activated TIS gene expression with similar induction kinetics. TPA pretreatment to deplete protein kinase C activity did not abolish the subsequent induction of TIS gene expression by epidermal growth factor or fibroblast growth factor; both peptide mitogens can activate TIS genes through a protein kinase C-independent pathway(s). We also analyzed TIS gene expression in three TPA-nonproliferative variants (3T3-TNR2, 3T3-TNR9, and A31T6E12A). The results indicate that (i) modulation of a TPA-responsive sodium-potassium-chloride transport system is not necessary for TIS gene induction either by TPA or by other mitogens and (ii) TIS gene induction is not sufficient to guarantee a proliferative response to mitogenic stimulation. ...
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascades involving MAPK1/3 (ERK1/2) and RAP1GAP. Depending on the cell type, is involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation. In cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 ...
TY - JOUR. T1 - Characterization of the inhibition of interleukin 2 mRNA accumulation by 12-O-tetradecanoylphorbol-13-acetate in primary lymphocytes. AU - Garlisi, C. G.. AU - Mastro, A. M.. PY - 1992. Y1 - 1992. N2 - We found previously that bovine lymph node cells (LNC) incubated with 12- O-tetradecanoylphorbol-13-acetate (TPA) for 18 h proliferate only to a limited degree on subsequent stimulation with concanavalin A (Con A), or with the comitogenic combination of Con A plus TPA. The lack of proliferation was traced to a lack of secretion of interleukin 2 (IL-2). Lack of secretion was paralled by a decrease in IL-2 mRNA levels. In this study we further characterized how TPA pretreatment affected IL-2 mRNA production. We found that TPA depressed IL-2 mRNA accumulation in a dose-dependent manner after at least 10 h of pretreatment. In contrast, pretreatment from 4 to 6 h augmented IL-2 mRNA accumulation. Furthermore, LNC stimulated with ionomycin plus TPA were less susceptible to inhibition by ...
Effect of PMA treatment on the expression of cyclins and cdks in IEC-18 cells. Cells were exposed to 100 nM PMA for the indicated times (U, untreated) and subje
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TY - JOUR. T1 - Comparison of M-line and other myofibril components during reversible phorbol ester treatment.. AU - Doetschman, T. C.. AU - Eppenberger, H. M.. PY - 1984/3/1. Y1 - 1984/3/1. N2 - The events occurring during phorbol ester mediated destruction of myofibrils in differentiated muscle cells were followed at the fluorescence and electron microscope levels using antibodies which bind troponin-T, a newly discovered 185 000 dalton M-line protein called myomesin and muscle type creatine kinase. The following series of events is proposed. Within one day of phorbol ester treatment, Z-bands and thin filaments, including troponin-T, are absent from many myofibrils resulting in the rapid loss of longitudinal and lateral alignment. A-bands become randomly oriented and clustered into ever smaller compartments within the rounding, myosac-like, multinucleated cells until after 3 days of treatment they too disappear. The M-line proteins are always present in existing A-bands. These results suggest ...
The present studies were undertaken to determine whether the CDKI FP could enhance PMA-induced maturation in human leukemia cells. The rationale for this investigation stemmed from several considerations: (a) FP has been shown to induce differentiation in some cell types (e.g., non-small cell lung cancer cells; Ref. 21 ); and (b) inhibition of cell cycle progression by FP might promote a leukemic cell differentiation program (47) . Contrary to expectations, coexposure to FP for 24 h strikingly opposed PMA-induced differentiation in U937 cells and instead significantly increased apoptosis. These events were associated with increased mitochondrial dysfunction, activation of caspases, and loss of clonogenic survival; moreover, enhanced cell death after PMA/FP cotreatment was also observed in promyelocytic leukemia cells (HL-60) and in U937 cells overexpressing the antiapoptotic protein Bcl-2. These events may reflect the complex reciprocal relationship that exists between differentiation and ...
Fingerprint Dive into the research topics of Enhanced vascular reactivity to protein kinase C activators in genetically hypertensive rats. Together they form a unique fingerprint. ...
Recent studies have indicated a link between levels of cyclooxygenase-2 (COX-2) and development of the multidrug resistance (MDR) phenotype. The ATP-binding cassette sub-family G member 2 (ABCG2) is a major MDR-related transporter protein that is frequently overexpressed in cancer patients. In this study, we aimed to evaluate any positive correlation between COX-2 and ABCG2 gene expression using the COX-2 inducer 12-O-tetradecanoylphorbol-13-acetate (TPA) in human breast cancer cell lines. ABCG2 mRNA and protein expression was studied using real-time RT-PCR and flow cytometry, respectively. A significant increase of COX-2 mRNA expression (up to 11-fold by 4 h) was induced by TPA in MDA-MB-231 cells, this induction effect being lower in MCF-7 cells. TPA caused a considerable increase up to 9-fold in ABCG2 mRNA expression in parental MCF-7 cells, while it caused a small enhancement in ABCG2 expression up to 67 % by 4 h followed by a time-dependent decrease in ABCG2 mRNA expression in MDA-MB-231 cells.
In the present study, ISL, a flavonoid isolated from licorice, was revealed to be a potent therapeutic agent for ACC. Although this natural flavonoid has been extensively considered as an antineoplastic agent in various human cancers (Hsu et al., 2005; Yoshida et al., 2008; Ye et al., 2009) and has shown inhibitory effects on the phorbol myristate acetate-induced angiogenic process of endothelial cells (Kang et al., 2010), this is the first study regarding prevention of tumor angiogenesis as an important component of the antitumor mechanisms of ISL. In this study, we demonstrated for the first time that ISL was a potent inhibitor of tumor-induced angiogenesis in ACC. Initially, by using growth analysis and viability measurement, we determined that ISL at a concentration of 0 to 20 μM did not induce significant ACC cell death but only effectively decreased the cell growth activity. Further investigation revealed that ISL at these concentrations not only significantly prevented ACC-mediated ...
Using oligonucleotide microarray, we have identified and cloned a novel gene that was induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in CD18 pancreatic cancer cells. In-silico analysis suggested localization of the gene product to the endoplasmic reticulum, thus we named it TPA induced Trans-Membrane Protein (TTMP). We found that TTMP is highly expressed in normal human pancreas, but has low expression in cancer cell lines. Confocal immunofluorescence microscopy localized TTMP to the endoplasmic reticulum. CD 18 and HeLa cells stably expressing TTMP inhibited cell proliferation. Conversely, siRNA duplexes targeted to TTMP in CD18 cells led to an increase in cell proliferation, as did clones expressing an in-frame N-terminal truncation of TTMP. Cell cycle analysis showed that TTMP induced a G1 phase arrest in pancreatic cancer cells. Finally, the promoter of TTMP was cloned, and basal activity was found to be dependent on Sp1 ...
The protein kinase C (PKC) activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) activated cell death in androgen-sensitive LNCaP cells but not in androgen-independent DU-145 or PC-3 cells, whose growth was significantly decreased by PKC inhibitors staurosporine and H7. All cell lines had similar le …
Heparin is a potent inhibitor of the proliferation and migration of vascular smooth muscle cells. This agent selectively inhibits the transcription of tissue-type plasminogen activator and interstitial collagenase, probably by decreasing the binding of activator protein-1 (AP-1) to phorbol ester-responsive elements in the promoters of these genes. Decreased AP-1 binding is not due to a direct inhibition by heparin, since heparinase digestion of nuclear extracts prepared from heparin-treated smooth muscle cells does not restore AP-1 binding activity. Treatment of cells with heparin suppresses the expression of Jun B, one of the components of AP-1. The major effect of heparin is at the level of posttranslational modification of Jun B. Results from pulse-chase labeling experiments show that the newly synthesized Jun B is rapidly converted to a higher-molecular-weight form and that conversion is suppressed by heparin. Evidence is presented suggesting that the heparin-inhibited event is ...
TY - JOUR. T1 - Reversal of defective IL-6 production in lipopolysaccharide-tolerant mice by phorbol myristate acetate. AU - Mengozzi, Manuela. AU - Sironi, Marina. AU - Gadina, Massimo. AU - Ghezzi, Pietro. PY - 1991/8/1. Y1 - 1991/8/1. N2 - The development of LPS tolerance has been suggested to be mediated by an inhibition of cytokine synthesis. Here we have studied serum IL-6 and TNF levels in mice after LPS administration. Repeated administration of LPS (35 μg daily for 4 days) to mice induced a refractoriness (tolerance) to subsequent administrations of LPS in terms of induction of circulating IL-6 and TNF. To investigate the mechanism by which LPS down-regulates its own induction of cytokine synthesis and the relationship between IL-6 and TNF production, we attempted to revert the inhibition of IL-6 and TNF production using agents like PMA or IFN-γ, previously reported to activate macrophage production of cytokines. Pretreatment with PMA (4 μg, 10 min before LPS) partially restored IL-6 ...
cytosolic-nuclear tumor promoter binding protein: binds tumor promoters such as tetradecanoylphorbol-13 acetate, teleocidins, aplysiatoxin & thapsigargin; MW 70 kDa existing in cytosolic fraction as a complex with the 90 kDa heat-shock protein; natural ligand is yakkasterone; amino acid sequence has been determined
Activation of PPI hydrolysis by carbachol elicits a robust translocation of CaM from membranes into cytosol which was previously shown to be mimicked by the addition of the calcium ionophore ionomycin and the phorbol ester TPA28 ...
T cell activation via the T cell receptor (T3-Ti complex) by OKT3 results in modulation of the T3-Ti complex, but does not affect T4, T8, or T11 antigen expression. To study the effect of other T cell activators on these T cell membrane antigens, the authors incubated mononuclear cells for 0-3 days with lectins or pharmacologic agents and stained with monoclonal antibodies to their antigens. The median fluorescence intensity (MFI) was measured with a fluorescence activated cell sorter. Activation of PBL with Con A, PHA, calcium ionophore A23187, or with dbcAMP, isoproterenol, or theophyllin had minimal effects on the MFI of T3, T4, T8, or T11. Phorbol myristate acetate (PMA), a protein kinase C activator which stimulates PBL though an alternate pathway, caused a 90-100% reduction of T3 and T4 MFI, a 25% reduction in T8 MFI, and a 400% increase in T11 MFI after 2 days. Addition of A23187 slightly increased these effects. PMA induced a 2-3-fold increase in cell diameter concomitant with the ...
Carcinogenesis is caused by a cumulative, multistage process that mainly consists of initiation, promotion, and progression. ROS, which are produced as a result of the metabolism of molecular oxygen via biochemical reactions in cells, play a key role in tumor promotion. Some tumor promoters accelerate/induce the conversion of initiated cells (carcinogen-mediated mutation or potential stem cells) into tumorigenic cells possibly via the production of oxidative/inflammatory responses (30, 31). TPA (12-O-tetradecanoylphorbol-13-acetate), a phorbol ester, is a tumor promoter that induces the neoplastic/tumorigenic transformation of preneoplastic JB6 cells through the overproduction of ROS (32). In this study, we investigated the inhibitory effect of SFN on TPA-stimulated neoplastic transformation in the mouse epidermal JB6 P+ cell line to assess whether SFN is able to block tumor promoter-induced tumorigenesis in skin cells. Our results show that SFN was effective when it was given together with ...
Amplifier Denon PMA-800NE, PMA800NEB, The Denon PMA-800NE integrated amplifier sets new heights in premium audio performance with digital inputs and a built-in phono equalizer, From deep bass to detailed highs, listen to your favourite high-resolution audio content with the 192kHz/24bit D/A converter, At 85W (4 Ohms) per channel, the PMA-800NE supplies enough power to drive your speakers for optimal sound
Topical application of marine briarane-type diterpenes effectively inhibits 12-O-tetradecanoylphorbol-13-acetate-induced inflammation and dermatitis in murine skin. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Perecko T, Drabikova K, Rackova L, Ciz M, Podborska M, Lojek A, Harmatha J, Smidrkal J, Nosal R, Jancinova V. Molecular targets of the natural antioxidant pterostilbene: effect on protein kinase C, caspase-3 and apoptosis in human neutrophils in vitro. Neuro Endocrinol Lett. 2010 Jan; 31(Suppl 2): 84-90 ...
The responsiveness to IL-4 with and without costimulation with anti-IgM antibodies or phorbolester was studied in 35 cases of low grade non-Hodgkin Iymphoma by analyzing enhancement of CD23 and HLA dass li expression. The predominant phenotype responds directly to IL-4. Separate differentiation states can be distinguished according to coordinate or differential upregulation of CD23 and HLA dass II molecules by IL-4 alone, and differences in responsiveness to anti-IgM antibodies. A particular subgroup of B-lymphoma cells defines a separate stage of B-eeil differentiation. They fail to express high affinity binding sites for IL-4 and accordingly do not respond to IL-4- mediated signals. Cross-linking membrane lgM receptors or direct activation of protein kinase C via phorbolester induces IL-4 receptor expression and subsequent IL-4 reactivity ...
The responsiveness to IL-4 with and without costimulation with anti-IgM antibodies or phorbolester was studied in 35 cases of low grade non-Hodgkin Iymphoma by analyzing enhancement of CD23 and HLA dass li expression. The predominant phenotype responds directly to IL-4. Separate differentiation states can be distinguished according to coordinate or differential upregulation of CD23 and HLA dass II molecules by IL-4 alone, and differences in responsiveness to anti-IgM antibodies. A particular subgroup of B-lymphoma cells defines a separate stage of B-eeil differentiation. They fail to express high affinity binding sites for IL-4 and accordingly do not respond to IL-4- mediated signals. Cross-linking membrane lgM receptors or direct activation of protein kinase C via phorbolester induces IL-4 receptor expression and subsequent IL-4 reactivity ...
I have started measuring in-situ Protein Kinase C activity in permeabilized cells grown in 96-well plates, based on a few references from the literature such as: Heasley L.E., J.Biol.Chem., 1989, 264, 8646. I always get high activity after stimulation with phorbol esters and very low activity after treatment with PKC inhibitors. The problem is that the activity in untreated cells is often almost as high as in stimulated cells. Any advice or protocol would be welcome. Thanks Bernard medbpl at emory.edu ...
During activation, PKC isoforms translocate to the plasma membrane (Nishizuka, 1984), and are autophosphorylated at multiple amino acid residues (Keranen et al., 1995; Newton, 2003). We attempted to measure PKC activation directly via: 1) PKCδ immunostaining (Fig. 6A), 2) membrane/cytosol fractionation and PKCδ Western blotting (Brown et al., 2005), 3) anti-phospho-PKCδ Western blotting (Sumandea et al., 2008), 4) anti-pan-phospho-PKC Western blotting (Iwabu et al., 2004), 5) a GFP biosensor based on the C1 domains of PKCγ (Oancea et al., 1998), and 6) a FRET biosensor of PKC activity (Violin et al., 2003). Unfortunately, we did not observe endogenous PKC activation with any of these techniques after stimulating endogenous M3 receptors with carbachol, and were therefore unable to directly measure the effect of DGKη on endogenous PKC activity. This did not reflect technical limitations, as we did observe endogenous PKCδ activation after direct stimulation with PMA (Fig. 6A). Instead, our ...
We have previously shown that multiple topical applications, over 11 days, of the phorbol ester 12- O-tetradecanoylphorbol-13-acetate (TPA) induces a persistent inflammatory reaction characterized by...
Viktige forbindelser for romstasjonen, slike som ulike væsker, miljøkontroll og livsstøtte-systemer, elektriske og datasystemer går igjennom Unity for å forsyne arbeids- og boligområdene på romstasjonen. Mer enn 50 000 mekaniske deler, 216 ledninger for å frakte væsker og gass og 121 interne og eksterne elektriske kabler, er installert i modulen. Unity er laget av aluminium. Før oppskyting ombord i Endeavour ble «Pressurized Mating Adapters» (PMA-1 og PMA-2) montert på koblingsmekanismene foran og bak på Unity. Disse adapterne tillater bruk av både de amerikanske romfergene og russiske moduler. PMA-1 forbinder nå permanent Unity med modulen Zarja. PMA-2 brukes for tilkobling av romfergen. Koblet til utsiden av PMA-1 er datamaskiner, eller «multiplexer-demultiplexers» (MDMs), som sørget for den tidlige kontrollen over Unity. Unity er også utstyrt med et kommunikasjonssystem som tillater data, lyd og lavhastighets data video-overføringer til Houston. Dette var ment som et ...
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Aldrich-547158; trans,trans-2,4-Hexadienyl acetate 0.97; CAS No.: 1516-17-2; Synonyms: Sorbyl acetate; Linear Formula: CH3(CH=CH)2CH2OC(O)CH3; Empirical Formula: C8H12O2; find related products, papers, technical documents, MSDS & more at Sigma-Aldrich.
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In vitro studies have shown that the phorbol ester, 12-tetradecanoylphorbol 13-acetate (TPA) induces neural crest cell differentiation into melanocytes, and stimulates proliferation and differentiation of normal melanocytes. As TPA is not a physiological agent, its action is clearly mimicking some in vivo pathway involved in these processes. An understanding of the effect of TPA on the expression of melanogenic genes will therefore provide valuable insight into the molecular mechanisms regulating melanocyte differentiation. In this study, we utilized primary cultures of neural crest cells and an immortalized melanocyte cell line (DMEL-2) which proliferates in the absence of TPA, to explore the effects of TPA on key melanogenic effectors. In neural crest cells, TPA was found to be necessary for both microphthalmia associated transcription factor (Mitf) up-regulation and for melanin synthesis. Using northern blots, we show that in DMEL-2 cells, TPA significantly increases the messenger ribonucleic acid
Phorbol ester-sensitive EL4 murine thymoma cells respond to phorbol 12-myristate 13-acetate with activation of ERK mitogen-activated protein kinases, synthesis of interleukin-2, and death, whereas phorbol ester-resistant variants of this cell line do not exhibit these responses. Additional aspects of the resistant phenotype were examined, using a newly-established resistant cell line. Phorbol ester induced morphological changes, ERK activation, calcium-dependent activation of the c-Jun N-terminal kinase (JNK), interleukin-2 synthesis, and growth inhibition in sensitive but not resistant cells. A series of protein kinase C activators caused membrane translocation of protein kinase Cs (PKCs) alpha, eta, and theta in both cell lines. While PKC eta was expressed at higher levels in sensitive than in resistant cells, overexpression of PKC eta did not restore phorbol ester-induced ERK activation to resistant cells. In sensitive cells, PKC activators had similar effects on cell viability and ERK ...
TY - JOUR. T1 - Selectivity of connexin 43 channels is regulated through protein kinase C-dependent phosphorylation. AU - Ek-Vitorin, Jose F.. AU - King, Timothy J.. AU - Heyman, Nathanael S.. AU - Lampe, Paul D.. AU - Burt, Janis M.. PY - 2006/6. Y1 - 2006/6. N2 - Coordinated contractile activation of the heart and resistance to ischemic injury depend, in part, on the intercellular communication mediated by Cx43-composed gap junctions. The function of these junctions is regulated at multiple levels (assembly to degradation) through phosphorylation at specific sites in the carboxyl terminus (CT) of the Cx43 protein. We show here that the selective permeability of Cx43 junctions is regulated through protein kinase C (PKC)-dependent phosphorylation at serine 368 (S368). Selective permeability was measured in several Cx43-expressing cell lines as the rate constant for intercellular dye diffusion relative to junctional conductance. The selective permeability of Cx43 junctions under control ...
Phorbol ester tumor promoters and the anti-tumor-promoter dexamethasone share a molecular target: modulation of the transcription factor AP-1 by novel type of ...
DDT1 MF-2 cells, which are derived from hamster vas deferens smooth muscle, contain alpha 1-adrenergic receptors (54,800 +/- 2700 sites per cell) that are coupled to stimulation of inositol phospholipid metabolism. Incubation of these cells with tumor-promoting phorbol esters, which stimulate calcium- and phospholipid-dependent protein kinase, leads to a marked attenuation of the ability of alpha 1-receptor agonists such as norepinephrine to stimulate the turnover of inositol phospholipids. This turnover was measured by determining the 32P content of phosphatidylinositol and phosphatidic acid after prelabeling of the cellular ATP pool with 32Pi. These phorbol ester-treated cells also displayed a decrease in binding affinity of cellular alpha 1 receptors for agonists with no change in antagonist affinity. By using affinity chromatography on the affinity resin Affi-Gel-A55414, the alpha 1 receptors were purified approximately equal to 300-fold from control and phorbol ester-treated 32Pi-prelabeled ...
TY - JOUR. T1 - Anti-inflammatory effects of licorice and roasted licorice extracts on TPA-induced acute inflammation and collagen-induced arthritis in mice. AU - Chung, Won Yoon. AU - Kim, Ki Rim. AU - Jeong, Chan Kwon. AU - Park, Kwang Kyun. AU - Choi, Jong Hoon. AU - Park, Jung Han Yoon. AU - Lim, Soon Sung. PY - 2010/5/6. Y1 - 2010/5/6. N2 - The anti-inflammatory activity of licorice (LE) and roated licorice (rLE) extracts determined in the murine phorbol ester-induced acute inflammation model and collagen-induced arthritis (CIA) model of human rheumatoid arthritis. rLE possessed greater activity than LE in inhibiting phorbol ester-induced ear edema. Oral administration of LE or rLE reduced clinical arthritis score, paw swelling, and histopathological changes in a murine CIA. LE and rLE decreased the levels of proinflammatory cytokines in serum and matrix metalloproteinase-3 expression in the joints. Cell proliferation and cytokine secretion in response to type II collagen or ...
TY - JOUR. T1 - Effect of bee venom and its fractions on the release of pro-inflammatory cytokines in PMA-differentiated U937 cells co-stimulated with LPS. AU - Tusiimire, Jonans. AU - Wallace, Jennifer. AU - Woods, Nicola. AU - Dufton, Mark J.. AU - Parkinson, John A.. AU - Abbott, Grainne. AU - Clements, Carol J.. AU - Young, Louise. AU - Park, Jin Kyu. AU - Jeon, Jong Woon. AU - Ferro, Valerie A.. AU - Watson, David G.. PY - 2016/4/19. Y1 - 2016/4/19. N2 - The venom of Apis mellifera (honey bee) has been reported to play a role in immunotherapy, but existing evidence to support its immuno-modulatory claims is insufficient. Four fractions from whole bee venom (BV) were separated using medium pressure liquid chromatography. Their ability to induce the production of cytokines TNFα, IL-1β and IL-6 in phorbol-12-myristate-13-acetate (PMA)-treated U937 cells was assessed. The levels of the three cytokines produced by stimulation with the four fractions and crude BV without LPS were not ...
The activity of calcium-, phospholipid-dependent protein kinase (PKc) was measured in (a) total extracts, (b) crude membrane, and (c) cytosolic fractions of chick embryo myogenic cells differentiating in culture. Total PKc activity slowly declines during the course of terminal myogenesis in contrast to the activity of cAMP-dependent protein kinase, which was also measured in the same cells. Myogenic cells at day 1 of culture possess high particulate and low soluble PKc activity. A dramatic decline of particulate PKc activity occurs during myogenic cell differentiation and is accompanied, through day 4, by a striking rise of the soluble activity. The difference in the subcellular distribution of PKc between replicating myoblasts and myotubes is confirmed by phosphorylation studies conducted in intact cells. These studies demonstrate that four polypeptides whose phosphorylation is stimulated by the tumor promoter 12-O-tetradecanoyl phorbol 13-acetate in myotubes, are spontaneously phosphorylated ...
AP-1 transcriptional activity is stimulated by the transformation promoters phorbol 12-myristate 13-acetate (12-O-tetradecanoylphorbol 13-acetate, TPA) and epidermal growth factor (EGF) in promotion-sensitive (P+) but not in promotion-resistant (P-) JB6 mouse epidermal cell lines. Although TPA stimulates expression of the jun and fos family genes, only c-jun expression shows higher elevation in P+ cells than in P- cells. The present study tests the hypothesis that induced AP-1 activity is required for tumor promoter-induced transformation in JB6 P+ cells. Both retinoic acid and the glucocorticoid fluocinolone acetonide inhibited basal and TPA-induced AP-1 activities that were tested with a stromelysin promoter-chloramphenicol acetyltransferase reporter gene in P+ cells. Since both retinoic acid and fluocinolone acetonide are active in inhibiting TPA-induced anchorage-independent transformation of P+ cells in the dose range that blocks TPA-induced AP-1 activity, their antipromoting effects may ...
TY - JOUR. T1 - Differential effects of protein kinase C on the levels of epithelial Na+ channel subunit proteins. AU - Stockand, James D. AU - Hui-Fang, B.. AU - Schenck, J.. AU - Malik, B.. AU - Middleton, P.. AU - Schlanger, L. E.. AU - Eaton, D. C.. PY - 2000/8/18. Y1 - 2000/8/18. N2 - Regulation of epithelial Na+ channel (ENaC) subunit levels by protein kinase C (PKC) was investigated in A6 cells. PKC activation altered ENaC subunit levels, differentially decreasing the levels of both β and γ, but not αENaC. Temporal regulation of β and γENaC by PKC differed; γENaC decreased with a time constant of3.7 ± 1.0 h, whereas βENaC decreased in 13.9 ±3.0h. Activation of PKC also resulted in a decrease in trans-epithelial Na+ reabsorption for up to 48h. PMA activation of PKC resulted in negative feedback inhibition of PKC protein levels beginning within 4h. Both β and γENaC levels, as well as transport tended toward pretreatment values after 48 h of PMA treatment. PKC inhibitors ...
RESULTS: Pterostilbene possessed comparable antioxidant properties as resveratrol in cell free system. Computational methods were used to establish the molecular characteristics of stilbene derivatives. The values of electronic parameters suggest a slight enhancement of electron donor properties of pterostilbene compared to resveratrol. Phosphorylation and thus activation of protein kinase C alpha/beta II in activated neutrophils was not decreased by pterostilbene. Pterostilbene in concentrations of 10-100 μM was found to inhibit the activity of human caspase-3 purified enzyme and did not influence cell viability significantly ...
TY - JOUR. T1 - Regulation of fibronectin gene expression by cyclic AMP and phorbol myristate acetate in HT-1080 human fibrosarcoma cells. AU - Lee, Byung Heon. AU - Park, Rang Woon. AU - Kim, In San. PY - 1998/12/31. Y1 - 1998/12/31. N2 - We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.. AB - We studied the regulation of ...
FIG. 3. Expression of PKC-α, -βI, and -βII isoforms in membrane and cytosolic fractions of DRG in experimental animals. Western blot analysis showed a single band of each isoform in all groups (A). Compared with nondiabetic littermate control mice (Lm) and transgenic mice (Tg), densitometric analysis disclosed reduced expression of membrane α isoform in both diabetic littermate mice (LmDM) and diabetic transgenic mice (TgDM), and the change in diabetic transgenic mice was more severe than in diabetic littermate mice (B). By contrast, cytosolic fraction of α isoform was contrariwise increased to a similar extent in both diabetic transgenic mice and diabetic littermate mice. Treatment with an ARI (fidarestat) corrected these changes in both diabetic groups (LmDM+ARI and TgDM+ARI). There was no change in βI expression in either membrane or cytosolic fraction among all groups. On the other hand, membrane βII expression tended to be elevated in diabetic littermate mice, and the increase was ...
B lymphocytes are necessary cells in defense replies. B lymphocytes HVCN1S appearance is normally higher in B-cell lines and in B cells from sufferers with chronic lymphocytic leukemia where it could donate to disease pathogenesis. and and relationship did not differ significantly. HVCN1S Responds More Strongly Avasimibe Avasimibe to PKC-Dependent Phosphorylation. Proton currents in phagocytes and other cells are greatly augmented by phosphorylation of the channel by PKC (8). The enhanced gating response is usually stimulated effectively by the PKC activator PMA (phorbol myristate acetate) and is best analyzed using the perforated-patch configuration that preserves intracellular signaling pathways (9). Fig. 2 illustrates families of proton currents in cells expressing HVCN1L and HVCN1S before and after PMA activation. In response to PMA the currents turn on more rapidly and at more unfavorable voltages and turn off more slowly and the current amplitude is usually increased. Although HVCN1L ...
Quercetin (QUE; 3,5,7,3′,4′-tetrahydroxyflavone) has been shown to possess several beneficial biological activities including antitumor, anti-inflammation and antioxidant properties; however, the effects of QUE in preventing invasion by breast carcinoma cells are still undefined. Increases in the protein, messenger RNA and enzyme activity levels of matrix metalloproteinase (MMP)-9 were observed in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated MCF-7 cells, and these were blocked by QUE, but not by quercitrin or rutin. A translocation of protein kinase C (PKC)δ from the cytosol to the membrane followed by activation of extracellular signal-regulated kinase (ERK) and c-Jun/activator protein-1 (AP-1) by TPA was demonstrated, and TPA-induced MMP-9 activation and migration were inhibited by the pan PKC inhibitor, GF109203X, the specific PKCδ inhibitor, rottlerin, an ERK inhibitor (PD98059) and an AP-1 inhibitor (curcumin). Application of QUE significantly suppressed TPA-induced activation ...
Author: Rauscher, Andreas et al.; Genre: Journal Article; Published in Print: 2000-09-07; Keywords: Vimentin; Gene expression; Electroporation; Stress; 12-O-Tetradecanoylphorbol-13-acetate; Plasmacytoma; MPC-11 cell; Title: Similar effects of electroporational stress and treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate on vimentin expression in mouse plasmacytoma cells
Although Fc receptors (FcRs) for switched immunoglobulin (Ig) isotypes have been extensively characterized, FcR for IgM (FcµR) has defied identification. By retroviral expression and functional cloning, we have identified a complementary DNA (cDNA) encoding a bona fide FcµR in human B-lineage cDNA libraries. FcµR is defined as a transmembrane sialoglycoprotein of ~60 kD, which contains an extracellular Ig-like domain homologous to two other IgM-binding receptors (polymeric Ig receptor and Fc/µR) but exhibits an exclusive Fcµ-binding specificity. The cytoplasmic tail of FcµR contains conserved Ser and Tyr residues, but none of the Tyr residues match the immunoreceptor tyrosine-based activation, inhibitory, or switch motifs. Unlike other FcRs, the major cell types expressing FcµR are adaptive immune cells, including B and T lymphocytes. After antigen-receptor ligation or phorbol myristate acetate stimulation, FcµR expression was up-regulated on B cells but was down-modulated on T cells, ...
Spirulina, a water blue-green microalga, is considered a complex natural product that is widely used in treatment of chronic diseases including cancer, hypercholesterolemia, arterial hypertension, obesity and diabetes. Phycocyanin from spirulina is considered to be a strong radical scavenger (hydroxyl, peroxyl and alkoxyl radicals) providing significant antioxidant and anti-inflammatory effects. The aim of this study consists in the evaluation of the anti-inflammatory effect of SP in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear inflammation in hairless SKH1 mice. The ears of mice treated with a higher concentration of SP (1000 μg/mL) showed a significant reduction of the inflammatory process than those treated with a smaller concentration of SP (200 μg/mL). Consequently, spirulina has proved dose-dependent anti-inflammatory effects in controlling and, also, in improving the acute inflammation process in mice, being a future alternative therapy for treating inflammation diseases ...
Spirulina, a water blue-green microalga, is considered a complex natural product that is widely used in treatment of chronic diseases including cancer, hypercholesterolemia, arterial hypertension, obesity and diabetes. Phycocyanin from spirulina is considered to be a strong radical scavenger (hydroxyl, peroxyl and alkoxyl radicals) providing significant antioxidant and anti-inflammatory effects. The aim of this study consists in the evaluation of the anti-inflammatory effect of SP in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear inflammation in hairless SKH1 mice. The ears of mice treated with a higher concentration of SP (1000 μg/mL) showed a significant reduction of the inflammatory process than those treated with a smaller concentration of SP (200 μg/mL). Consequently, spirulina has proved dose-dependent anti-inflammatory effects in controlling and, also, in improving the acute inflammation process in mice, being a future alternative therapy for treating inflammation diseases ...
The expression of proteases such as MMP-9 is regulated by diverse growth factors, cytokines, and xenobiotics such as PMA. Studies have shown that the mechanism responsible for PMA-mediated responses may involve direct alteration of transcription factors, but these mechanisms are not completely understood. Small molecular weight inhibitors that target the pathways that regulate MMP-9 expression could improve our understanding of these pathways and potentially be of clinical utility for the treatment of cancer. Using a cervical cancer cell line, our study demonstrates the ability of dykellic acid to reduce the expression of MMP-9.. We also investigated the molecular mechanism by which dykellic acid inhibits PMA-mediated expression of MMP-9 using AP-1 and NFκB reporter constructs and found that NFκB activity, but not AP-1 activity, is significantly reduced by treatment with dykellic acid. Thus, dykellic acid suppresses expression of MMP-9 via inhibition of NFκB transactivation. To our knowledge, ...
In this study, short-term phorbol ester exposure was found to prevent acute AA + Fe (also AA alone or iron alone) oxidative stress and toxicity in the CYP2E1-expressing E47 cells. 4-α-TPA, a TPA-derived biologically inactive molecule unable to activate PKC, was not protective. Accordingly, the mechanism by which TPA exerts its protection seems to be related to its ability to activate certain PKC isoform(s) (Nishizuka, 1984) rather than via a direct effect as an antioxidant molecule. The protective effect of TPA on CYP2E1-mediated AA + Fe toxicity in E47 cells was dose- and time-dependent and occurred simultaneously with an increased translocation of phosphorylated PKC to membranes.. Inhibitors of PKC can interact with the ATP/substrate-binding sites or with regulatory sites of the enzyme. TPA-stimulated PKC isoform(s) involved in the protective action of this phorbol ester on CYP2E1-dependent AA + Fe toxicity in E47 cells were sensitive to the PKC inhibitors Ro 31-8425 and staurosporine, which ...
adducin: membrane-skeleton associated calmodulin-binding protein of erythrocytes; major substrate for Ca+- & phospholipid-dependent protein kinase C; alpha-beta heterodimer with subunits of MW 103kDa (alpha) & 97kDa (beta); human erythrocyte GenBank X58199; alternatively spliced human transcript GenBank U43959
Effects of PKC activators on ACh-induced increases in [Ca2+]i. Fura-2 loaded endothelial cells were treated with ACh (3 μmol/L) followed by washing. Cells were
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Sigma-Aldrich offers abstracts and full-text articles by [Shinichi Meguro, Noriko Osaki, Koji Onizawa, Noriyuki Yajima, Tadashi Hase, Noboru Matsuo, Ichiro Tokimitsu].
The key difference between methyl acetate and ethyl acetate is that methyl acetate has a methyl group attached to an acetate group whereas ethyl acetate ha
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I cut an 8 1/2 x 11 to 4.25 x 11. Scored at 5.5, 7.25 and 9. The second and third score lines are were you are going to cut out your center panel. Save your bottom piece when you cut , you will reattach to the acetate panel. For the acetate panel I cut a 2.5 x 4.25. attach to inside of card bring in 1/2 to adhere to card. Take your bottom panel and reattach bringing in 1/2 to attache. Whala, you now have an acetate panel card ...
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TPA (12-O-Tetradecanoylphorbol-13-acetate), a potent PKC activator, induces mS100a7a15 expression. TPA induces mS100a7a15 ...
TPA (12-O-Tetradecanoylphorbol-13-acetate) is a tumour promoter used in biomedical research. EBV-EA is Epstein-Barr virus early ...
Phorbol 12-myristate 13-acetate is also known as 12-O-tetradecanoylphorbol-13-acetate (TPA). TASK channels are additionally ... and strongly inhibited by phorbol 12-myristate 13-acetate. ...
... a gene induced in Swiss 3T3 cells by the tumor promoter tetradecanoyl phorbol acetate". Oncogene. 4 (10): 1263-5. PMID 2797820 ...
JDP2 regulates 12-O-tetradecanoylphorbol-13-acetate (TPA) response element (TRE)- and cAMP-responsive element (CRE)-dependent ...
... activation by 12-O-tetradecanoylphorbol-13-acetate". The Journal of Investigative Dermatology. 100 (1): 10-5. doi:10.1111/1523- ...
Kim YJ, Pan H, Verma AK (July 1994). "Non-AP-1 tumor promoter 12-O-tetradecanoylphorbol-13-acetate-responsive sequences in the ...
In particular, 12-O-tetradecanoylphorbol-13-acetate (TPA) is used as a biomedical research tool in models of carcinogenesis. ... "Tumour promoter phorbol-12-myristate-13-acetate induces chromosomal damage via indirect action". Nature. 293 (5828): 144-6. ...
"Ultrastructural Analysis of Epidermal Hyperplasia Induced by Multiple 12-0-Tetradecanoyl-Phorbol-13-Acetate (TPA) Treatment of ...
As an activator of protein kinase C, it is a weak tumor promoter compared to 12-O-tetradecanoylphorbol-13-acetate. PDBu is ...
Tumor promotion can be induced with treatments of 12-O-tetradecanoylphorbol-13-acetate (TPA) in some models of two-stage ...
Expression of miR-22 can be induced by adding 12-O-Tetradecanoylphorbol-13-acetate (TPA) to HL-60 cells (leukaemia cell line). ...
"Identification by differential display of a mRNA specifically induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in T84 ...
Chang PL, Prince CW (May 1991). "1 alpha,25-Dihydroxyvitamin D3 enhances 12-O-tetradecanoylphorbol-13-acetate- induced ...
... of nuclear factor kappaB in up-regulation of aldose reductase gene expression by 12-O-tetradecanoylphorbol-13-acetate in HeLa ...
"Inhibitory effects of sterols isolated from Chlorella vulgaris on 12-O-tetradecanoylphorbol-13-acetate-induced inflammation and ...
"A variant CD30 protein lacking extracellular and transmembrane domains is induced in HL-60 by tetradecanoylphorbol acetate and ...
... such as 12-O-tetradecanoylphorbol-13-acetate. In the Amazon, the red latex from the species C. lechleri, known as sangre de ...
April 1996). "Inhibitory effects of caffeic acid phenethyl ester (CAPE) on 12-O-tetradecanoylphorbol-13-acetate-induced tumor ... and ferulic acid on tumor promotion in mouse skin by 12-O-tetradecanoylphorbol-13-acetate". Cancer Res. 48 (21): 5941-6. PMID ...
... significantly inhibited tumor promotion caused by 12-O-tetradecanoylphorbol-13-acetate (TPA, 1.6 nmol). This article ...
The most common and potent phorbol ester is 12-O-tetradecanoylphorbol-13-acetate (TPA), also called phorbol-12-myristate-13- ... Tseng SS, van Duuren BL, Solomon JJ (1977). "Synthesis of 4aα-Phorbol 9-Myristate 9a-Acetate and Related Esters". J. Org. Chem ... pentacyclic triterpene α-amyrin in the mouse skin inflammation induced by phorbol ester 12-O-tetradecanoylphorbol-13-acetate". ... acetate (PMA), which is used as a biomedical research tool in contexts such as models of carcinogenesis. Phorbol is a natural ...
"Comparison of the effects of bilobol and 12-O-tetradecanoylphorbol-13-acetate on skin, and test of tumor promoting potential of ...
... complete amino acid sequence of a protein produced by the 12-0-tetradecanoylphorbol-13-acetate-treated human breast ...
1990). "Comparison of the effects of bilobol and 12-O-tetradecanoylphorbol-13-acetate on skin, and test of tumor promoting ...
The AP-1 binding site was identified as the 12-O-Tetradecanoylphorbol-13-acetate (TPA) response element (TRE) with the ...
... tetradecanoyl phorbol acetate-inducible sequence 21) protein in mouse. Tis21 had been originally isolated as a sequence induced ...
CS1 maint: discouraged parameter (link) "Synergistic stimulatory effect of 12-O-tetradecanoylphorbol-13-acetate and capsaicin ...
Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 to ...
... the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), cAMP, vitamin D3, estrogen and tamoxifen, angiotensin II, hypoxia ...
... that allixin exerts an anti-promoting activity against skin tumors induced by the chemical 12-O-tetradecanoylphorbol-13-acetate ...
Lithium (lithium acetate, lithium carbonate, lithium chloride, lithium citrate, lithium hydroxide, lithium orotate) ...
The hormone prolactin stimulates lactation (production of breast milk). Dopamine, released by the hypothalamus stops the release of prolactin from the pituitary gland. Domperidone, by acting as an anti-dopaminergic agent, results in increased prolactin secretion, and thus promotes lactation (that is, it is a galactogogue). In some nations, including Australia, domperidone is used off-label, based on uncertain and anecdotal evidence of its usefulness, as a therapy for mothers who are having difficulty breastfeeding.[24][25] In the United States, domperidone is not approved for this or any other use.[26][27] A study called the EMPOWER trial was designed to assess the effectiveness and safety of domperidone in assisting mothers of preterm babies to supply breast milk for their infants.[28] The study randomized 90 mothers of preterm babies to receive either domperidone 10 mg orally three times daily for 28 days (Group A) or placebo 10 mg orally three times daily for 14 days followed by domperidone ...
... (DHP) is a molecule based upon pyridine, and the parent of a class of molecules that have been semi-saturated with two substituents replacing one double bond. They are particularly well known in pharmacology as L-type calcium channel blockers, used in the treatment of hypertension. Compared with certain other L-type calcium channel blockers (for example those of the phenylalkylamine class such as verapamil) that have significant action at the heart, they are relatively vascular selective in their mechanism of action in lowering blood pressure. ...
InChI=1S/C20H27N5O5S/c1-15-14-18(23-30-15)19(26)21-11-10-16-6-8-17(9-7-16)31(28,29)24-20(27)22-25-12-4-2-3-5-13-25/h6-9,14H,2-5,10-13H2,1H3,(H,21,26)(H2,22,24,27) ...
Lithium (lithium acetate, lithium carbonate, lithium chloride, lithium citrate, lithium hydroxide, lithium orotate) ...
A channel that is "inwardly-rectifying" is one that passes current (positive charge) more easily in the inward direction (into the cell) than in the outward direction (out of the cell). It is thought that this current may play an important role in regulating neuronal activity, by helping to stabilize the resting membrane potential of the cell. By convention, inward current (positive charge moving into the cell) is displayed in voltage clamp as a downward deflection, while an outward current (positive charge moving out of the cell) is shown as an upward deflection. At membrane potentials negative to potassium's reversal potential, inwardly rectifying K+ channels support the flow of positively charged K+ ions into the cell, pushing the membrane potential back to the resting potential. This can be seen in figure 1: when the membrane potential is clamped negative to the channel's resting potential (e.g. -60 mV), inward current flows (i.e. positive charge flows into the cell). However, when the ...
1990). "Comparison of the effects of bilobol and 12-O-tetradecanoylphorbol-13-acetate on skin, and test of tumor promoting ...
... also binds to and blocks α2δ subunit-containing VDCCs, similarly to gabapentin and pregabalin, and hence is a gabapentinoid.[9][16] Both (R)-phenibut and (S)-phenibut display this action with similar affinity (Ki = 23 and 39 μM, respectively).[9] Moreover, (R)-phenibut possesses 4-fold greater affinity for this site than for the GABAB receptor (Ki = 92 μM), while (S)-phenibut does not bind significantly to the GABAB receptor (Ki , 1 mM).[9] As such, based on the results of this study, phenibut would appear to have much greater potency in its interactions with α2δ subunit-containing VDCCs than with the GABAB receptor (between 5- to 10-fold).[9] For this reason, the actions of phenibut as a α2δ subunit-containing voltage-gated calcium channel blocker or gabapentinoid may be its true primary mechanism of action, and this may explain the differences between phenibut and its close relative baclofen (which, in contrast, has essentially insignificant activity as a gabapentinoid; Ki = 6 ...
InChI=1S/C24H34N2O/c1-21(2)19-27-20-24(25-15-9-10-16-25)18-26(23-13-7-4-8-14-23)17-22-11-5-3-6-12-22/h3-8,11-14,21,24H,9-10,15-20H2,1-2H3 ...
Lithium (lithium acetate, lithium carbonate, lithium chloride, lithium citrate, lithium hydroxide, lithium orotate) ...
Class Ib antiarrhythmic agents are sodium channel blockers. They have fast onset and offset kinetics, meaning that they have little or no effect at slower heart rates, and more effects at faster heart rates. Class Ib agents shorten the action potential duration and reduce refractoriness. These agents will decrease Vmax in partially depolarized cells with fast response action potentials. They either do not change the action potential duration, or they may decrease the action potential duration. Class Ib drugs tend to be more specific for voltage gated Na channels than Ia. Lidocaine in particular is highly frequency dependent, in that it has more activity with increasing heart rates. This is because lidocaine selectively blocks Na channels in their open and inactive states and has little binding capability in the resting state. Class Ib agents are indicated for the treatment of ventricular tachycardia and symptomatic premature ventricular beats, and prevention of ventricular fibrillation. Class Ib ...
... has anticonvulsive properties, attenuating vascular smooth muscle contraction through interactions with voltage-dependent Na+ and Ca2+ channels.[1] How this effect is mediated and to what extent this mechanism is involved in the anxiolytic and analgesic effects of kavalactones on the central nervous system is unknown. Kavain's pharmacological activities have not been sufficiently investigated, and neither its effect as a serotonin reuptake inhibitor nor its monoamine (norepinephrine) uptake inhibitions and activation of NMDA receptors have been confirmed. The mechanism behind the psychotropic, sedative, and anxiolytic actions of kavain and related kavalactones is still debated. Direct binding to the benzodiazepine/flumazenil binding site of the GABA-A receptor does not occur with kavain enantiomers.[2] Many studies involved kava extracts from different plant parts and are, therefore, not applicable to kavain itself. In 2016, kavain was shown to bind at the α4β2δ GABAA receptor and ...
... psoriatic lesion development upon stimulation with the lesion-causing tumor promoter 12-O-tetradecanoylphorbol-13-acetate.[6] ...
EBV in B cells can also be reactivated by treating the cells with sodium butyrate or 12-O-Tetradecanoylphorbol-13-acetate.[ ...
... occludes the pore of calcium-activated voltage-gated shaker K+ channels by binding to one of four independent, overlapping binding sites.[6][7] It binds both to the open and the closed states. In addition, the block is enhanced as the ionic strength is lowered.[8] This block occurs as the Asn 30 on the CTX interacts with the Asp 381 on the K+ channel.[9] The blockade of K+ channels by the charybdotoxin peptide causes neuronal hyperexcitability. Mutations of the Lys31Gln and the Asn30Gln had the effect of lessening the CTX block of the pore on the shaker channel.[9] ...
... selectively binds to sulfonylurea receptors (SUR-1) on the surface of the pancreatic beta-cells. It was shown to provide cardiovascular protection as it does not bind to sulfonylurea receptors (SUR-2A) in the heart.[10] This binding effectively closes these K+ ion channels. This decreases the efflux of potassium from the cell which leads to the depolarization of the cell. This causes voltage dependent Ca2+ ion channels to open increasing the Ca2+ influx. The calcium can then bind to and activate calmodulin which in turn leads to exocytosis of insulin vesicles leading to insulin release.[citation needed] The mouse model of MODY diabetes suggested that the reduced gliclazide clearance stands behind their therapeutic success in human MODY patients, but Urbanova et al. found that human MODY patients respond differently and that there was no consistent decrease in gliclazide clearance in randomly selected HNF1A-MODY and HNF4A-MODY patients.[11] Its classification has been ambiguous, as ...
... has several uses including the treatment of abnormal heart rhythms or arrhythmias, chronic pain, and myotonia. In general when treating arrhythmias, mexiletine is reserved for use in dangerous heart rhythm disturbances such as ventricular tachycardia.[2] It is of particular use when treating arrhythmias caused by long QT syndrome.[3] The LQT3 form of long QT syndrome is amenable to treatment with mexiletine as this form is caused by defective sodium channels that continue to release a sustained current rather than fully inactivating, however other forms of long QT syndrome can also be treated with this medication.[3] Mexiletine has been used to treat chronic pain and may also be used to treat muscle stiffness resulting from myotonic dystrophy (Steinert's disease) or nondystrophic myotonias such as myotonia congenita (Thomsen syndrome or Becker syndrome).[4][5] ...
Abiraterone acetate. *Alestramustine. *Almestrone. *Anabolic steroids (e.g., testosterone and esters, methyltestosterone, ...
... is effective for prevention of shivering during surgery or recovery from surgery.[5][6] Nefopam was significantly more effective than aspirin as an analgesic in one clinical trial,[7] although with a greater incidence of side effects such as sweating, dizziness and nausea, especially at higher doses.[8][9] The estimated relative potency of nefopam to morphine indicates that 20 mg of nefopam HCl is the approximate analgesic equal of 12 mg of morphine with comparable analgesic efficacy to morphine,[10][11][12] or oxycodone,[13] while Nefopam tends to produce fewer side effects, does not produce respiratory depression,[14] and has much less abuse potential, and so is useful either as an alternative to opioids, or as an adjunctive treatment for use alongside opioid(s) or other analgesics.[12][15] Nefopam is also used to treat severe hiccups.[16] ...
12-O-Tetradecanoylphorbol-13-acetate (Phorbol-12-myristate-13-acetate) GRCh38: Ensembl release 89: ENSG00000141682 - Ensembl, ... Phorbol-12-myristate-13-acetate-induced protein 1 is a protein that in humans is encoded by the PMAIP1 gene, and is also known ... "Entrez Gene: PMAIP1 phorbol-12-myristate-13-acetate-induced protein 1". Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, ... "Molecular cloning and characterization of a cDNA for a novel phorbol-12-myristate-13-acetate-responsive gene that is highly ...
12-O-Tetradecanoylphorbol-13-acetate (TPA), also commonly known as tetradecanoylphorbol acetate, tetradecanoyl phorbol acetate ... Tetradecanoylphorbol+Acetate at the US National Library of Medicine Medical Subject Headings (MeSH) "NCI Dictionary Entry". ... O-tetradecanoylphorbol-13-acetate for patients with relapsed/refractory malignancies". Cancer Chemotherapy and Pharmacology. 57 ... and phorbol 12-myristate 13-acetate (PMA), is a diester of phorbol and a potent tumor promoter often employed in biomedical ...
12-O-tetradecanoylphorbol-13-acetate listen (12-O-TEH-truh-DEH-kuh-noyl-FOR-bol-13-A-seh-tayt) A substance being studied in the ... 12-O-tetradecanoylphorbol-13-acetate affects many cell actions and may cause tumor cells to die. It is a type of phorbol ester ...
Recent studies have shown that topical application of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) to murine ... Inhibition of 12-O-tetradecanoylphorbol-13-acetate and other skin tumor-promoter-caused induction of epidermal interleukin-1 ...
12-O-tetradecanoylphorbol-13-acetate (TPA) in patients with relapsed or refractory. hematologic malignancies or bone marrow ... Patients receive 12-O-tetradecanoylphorbol-13-acetate (TPA) IV over 1 hour on days 1 and 8. followed by 2 weeks of rest. ... Phase I Study of 12-O-tetradecanoylphorbol-13-acetate (TPA) in Patients With Refractory Hematologic Malignancies/Bone Marrow ... Phase I Study of 12-O-tetradecanoylphorbol-13-acetate (TPA) in Patients With Refractory Hematologic Malignancies/Bone Marrow ...
Inhibition of 12-O-tetradecanoylphorbol 13-acetate-induced ornithine decarboxylase activity in mouse ME308 cells. ...
O-tetradecanoylphorbol 13-acetate.. By in vitro experiment monitoring with 12 - O - tetradecanoylphorbol-13-acetate-induced ... Inhibition of the Tumor-promoting Action of 12-O-Tetradecanoylphorbol-13-acetate by Some Oleanane-type Triterpenoid Compounds. ... Inhibition of the Tumor-promoting Action of 12-O-Tetradecanoylphorbol-13-acetate by Some Oleanane-type Triterpenoid Compounds ... Inhibition of the Tumor-promoting Action of 12-O-Tetradecanoylphorbol-13-acetate by Some Oleanane-type Triterpenoid Compounds ...
Tested for its antiinflammatory activity in the tetradecanoyl phorbol acetate induced ear edema model (TPA Ear). ...
We isolated a group of genes that are rapidly and transiently induced in 3T3 cells by tetradecanoyl phorbol acetate (TPA). ... Induction of tumor promotor-inducible genes in murine 3T3 cell lines and tetradecanoyl phorbol acetate-nonproliferative 3T3 ... Induction of tumor promotor-inducible genes in murine 3T3 cell lines and tetradecanoyl phorbol acetate-nonproliferative 3T3 ... Induction of tumor promotor-inducible genes in murine 3T3 cell lines and tetradecanoyl phorbol acetate-nonproliferative 3T3 ...
Induction of early-response genes (tetradecanoyl phorbol acetate [TPA]-induced sequences, or TIS genes; R.W. Lim, B.C. Varnum, ... Granulocyte-macrophage colony-stimulating factor and tetradecanoyl phorbol acetate induce a distinct, restricted subset of ... Granulocyte-macrophage colony-stimulating factor and tetradecanoyl phorbol acetate induce a distinct, restricted subset of ... Granulocyte-macrophage colony-stimulating factor and tetradecanoyl phorbol acetate induce a distinct, restricted subset of ...
2 The abbreviations used are: AR, androgen receptor; PKC, protein kinase C; TPA, 12-O-tetradecanoylphorbol acetate; NFκB, ... 12-O-tetradecanoylphorbolacetate (TPA) influences proliferation, differentiation, and apoptosis in a variety of cells including ... Interruption of Nuclear Factor κB Signaling by the Androgen Receptor Facilitates 12-O-Tetradecanoylphorbolacetate-Induced ... Interruption of Nuclear Factor κB Signaling by the Androgen Receptor Facilitates 12-O-Tetradecanoylphorbolacetate-Induced ...
Effects of the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate on the wasp Bracon hebetor.. код для вставки. код для ... Louis, MO). 20-[3H](N)-12-0-tetradecanoyl-phorbol-13-acetate with a specific activity of 250 pCilmmo1 was obtained from New ... Key words: anti-vitellogenic benzo(a)pyrene, braconid, habrobracon, TPA INTRODUCTION 12-0-Tetradecanoyl-phorbol-13-acetate has ... 12-O-Tetradecanoyl-phorbol-13acetate. Acknowledgments: The work presented here was supported by grant no. ES-07046 from the ...
The effects of tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), cAMP analogue dibutyryl cAMP (DB) and histamine were ... Changes in spermine levels are involved in the effects of phorbol ester (12-O-tetradecanoylphorbol-13-acetate) and dibutyryl ... Changes in spermine levels are involved in the effects of phorbol ester (12-O-tetradecanoylphorbol-13-acetate) and dibutyryl ... The effects of tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), cAMP analogue dibutyryl cAMP (DB) and histamine were ...
Induction of chromosomal alterations in primary mouse keratinocyte cultures by 12 o tetradecanoylphorbol 13 acetate but not 12 ... o retinoylphorbol 13 acetate and inhibition of the clastogenic effects by 4 8 11 14 eicosatetraynoic acid and antipain ... tetradecanoylphorbol 13 acetate use of 12 o tetradecanoylphorbol 13 acetate sensitive and 12 o tetradecanoylphorbol 13 acetate ... tetradecanoylphorbol-13-acetate in vivo. Carcinogenesis 8(1): 191-192, 1987. Inhibition of 12-O-tetradecanoylphorbol-13-acetate ...
We have studied the effects of the tumor promoter 12-O-tetradecanoylphorbol-13- acetate (TPA) in the differentiation phenotype ... The tumor promoter 12-O-tetradecanoylphorbol-13-acetate blocks differentiation of HT-29 human colon cancer cells ... The tumor promoter 12-O-tetradecanoylphorbol-13-acetate blocks differentiation of HT-29 human colon cancer cells ... The tumor promoter 12-O-tetradecanoylphorbol-13-acetate blocks differentiation of HT-29 human colon cancer cells ...
A tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), was found to increase the level of HPV18 transcripts in an HPV18- ... A tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), was found to increase the level of HPV18 transcripts in an HPV18- ... Enhancement of human papillomavirus type 18 gene expression in HeLa cells by 12-O-tetradecanoylphorbol-13-acetate, 3 beta,5 ... Enhancement of human papillomavirus type 18 gene expression in HeLa cells by 12-O-tetradecanoylphorbol-13-acetate, 3 beta,5 ...
N2 - We found previously that bovine lymph node cells (LNC) incubated with 12- O-tetradecanoylphorbol-13-acetate (TPA) for 18 h ... AB - We found previously that bovine lymph node cells (LNC) incubated with 12- O-tetradecanoylphorbol-13-acetate (TPA) for 18 h ... We found previously that bovine lymph node cells (LNC) incubated with 12- O-tetradecanoylphorbol-13-acetate (TPA) for 18 h ... abstract = "We found previously that bovine lymph node cells (LNC) incubated with 12- O-tetradecanoylphorbol-13-acetate (TPA) ...
Ultrastructural studies on cell fusion induced by Epstein-Barr virus or N-butyrate and 12-O-tetradecanoylphorbol-13-acetate. ...
Identification, characterization and expression analysis of a novel TPA (12-0-Tetradecanoylphorbol-13-Acetate) induced gene. ... we have identified and cloned a novel gene that was induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in CD18 pancreatic ... CHAN CHUNG YIP (2007-02-07). Identification, characterization and expression analysis of a novel TPA (12-0-Tetradecanoylphorbol ... Acetate) induced gene.. [email protected] Repository.. Abstract: Using oligonucleotide microarray, ...
keywords = "12-O-tetradecanoyl-phorbol-13-acetate, Apoptosis, Nasopharyngeal carcinoma, Proteomics",. author = "Peizhou Jiang ... T1 - Proteomics-based identification of proteins with altered expression induced by 12-O-tetradecanoylphorbol 13-acetate in ... Etiology studies indicate that chemical carcinogen promoters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA), are important ... title = "Proteomics-based identification of proteins with altered expression induced by 12-O-tetradecanoylphorbol 13-acetate in ...
The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the regulation of the estrogen receptor (ER) was investigated in ... Post-transcriptional destabilization of estrogen receptor mRNA in MCF-7 cells by 12-O-Tetradecanoylphorbol-13-acetate. Journal ... N2 - The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the regulation of the estrogen receptor (ER) was investigated ... AB - The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the regulation of the estrogen receptor (ER) was investigated ...
Furthermore, we explore the mechanism whereby exposure to retinoic acid (RA) and the phorbol ester 12-O-Tetradecanoylphorbol-13 ... acetate (TPA) can induce this cell line to differentiate into a stable population of human neurons, with significantly ... Tetradecanoylphorbol-13-acetate (TPA) significantly increases AAV2/5 transduction of human neuronal cells in vitro ... Tetradecanoylphorbol-13-acetate (TPA) significantly increases AAV2/5 transduction of human neuronal cells in vitro ...
We first determined the effect of topical application of GUG to mice against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ... We first determined the effect of topical application of GUG to mice against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ... We first determined the effect of topical application of GUG to mice against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ... was found to significantly suppress the invasion of cancer cells stimulated by the 12-O-tetradecanoyl-phorbol-13-acetate (TPA). ...
TETRADECANOYL PHORBOL ACETATE PRKCD Interaction Score: 3.75 Interaction Types & Directionality: n/a. ...
12-tetradecanoylphorbol 13-acetate (TPA) induces neural crest cell differentiation into melanocytes, and stimulates ... Stimulation of melanogenesis by tetradecanoylphorbol 13-acetate (TPA) in mouse melanocytes and neural crest cells. ... In vitro studies have shown that the phorbol ester, 12-tetradecanoylphorbol 13-acetate (TPA) induces neural crest cell ... Stimulation of melanogenesis by tetradecanoylphorbol 13-acetate (TPA) in mouse melanocytes and neural crest cells. ...
O-tetradecanoylphorbol-13-acetate (TPA) results in the formation of benign papillomas (PAPs) and malignant tumours (SCCs). ...
... and 12-O-tetradecanoylphorbol-13-acetate (TPA). These DMBA/TPA-induced squamous cell carcinomas … ... and 12-O-tetradecanoylphorbol-13-acetate (TPA). These DMBA/TPA-induced squamous cell carcinomas overexpress cyclooxygenase-2 ( ...
... activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) activated cell death in androgen-sensitive LNCaP cells but not in ... The protein kinase C (PKC) activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) activated cell death in androgen-sensitive ...
O-tetradecanoylphorbol-13-acetate (TPA) three times a week for 12 weeks. Mice were shaved before each treatment to allow a ...
  • 12-O-Tetradecanoylphorbol-13-acetate (TPA), also commonly known as tetradecanoylphorbol acetate, tetradecanoyl phorbol acetate, and phorbol 12-myristate 13-acetate (PMA), is a diester of phorbol and a potent tumor promoter often employed in biomedical research to activate the signal transduction enzyme protein kinase C (PKC). (wikipedia.org)
  • Induction of tumor promotor-inducible genes in murine 3T3 cell lines and tetradecanoyl phorbol acetate-nonproliferative 3T3 variants can occur through protein kinase C-dependent and -independent pathways. (asm.org)
  • We isolated a group of genes that are rapidly and transiently induced in 3T3 cells by tetradecanoyl phorbol acetate (TPA). (asm.org)
  • Granulocyte-macrophage colony-stimulating factor and tetradecanoyl phorbol acetate induce a distinct, restricted subset of primary-response TIS genes in both proliferating and terminally differentiated myeloid cells. (asm.org)
  • Effects of the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate on the wasp Bracon hebetor. (docme.ru)
  • Key words: anti-vitellogenic benzo(a)pyrene, braconid, habrobracon, TPA INTRODUCTION 12-0-Tetradecanoyl-phorbol-13-acetate has been widely studied in recent years because of its efficacy and potency as a tumor promotor in two-stage carcinogenesis studies with mice. (docme.ru)
  • 20-[3H](N)-12-0-tetradecanoyl-phorbol-13-acetate with a specific activity of 250 pCilmmo1 was obtained from New England Nuclear (Boston, MA). (docme.ru)
  • The protein kinase C (PKC) activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) activated cell death in androgen-sensitive LNCaP cells but not in androgen-independent DU-145 or PC-3 cells, whose growth was significantly decreased by PKC inhibitors staurosporine and H7. (nih.gov)
  • In this study, we used 5-aminolevulinic (ALA) acid and 3 water soluble photosensitizers-PP(Arg)(2), PP(Ser)(2)Arg(2), PP(Ala)(2)Arg(2), all diamino acid derivatives of protoporphyrin IX-to treat benign papillomas in FVB/N mice induced by 7,12-dimethylbenz(a)anthracene (DMBA)-12-O-tetradecanoyl-phorbol-13-acetate (TPA). (biomedsearch.com)
  • Composition of a chemopreventive proanthocyanidin-rich fraction from cranberry fruits responsible for the inhibition of 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced ornithine decarboxylase (ODC) activity. (cranberryinstitute.org)
  • Here, using dimethylbenzanthracene (DMBA) plus 12-o-tetradecanoyl phorbol-13-acetate (TPA) treatment developing sebaceous neoplasms (SNs) were identified with H&E and Oil red O staining. (medsci.org)
  • Previous studies also demonstrated that 12-O-tetradecanoyl-phorbol-13-acetate (TPA) can recruit HFSCs to maintain skin homeostasis and contribute to papilloma, squamous cell carcinoma and basal cell carcinoma [ 3 , 6 , 7 ]. (medsci.org)
  • In vitro studies have shown that the phorbol ester, 12-tetradecanoylphorbol 13-acetate (TPA) induces neural crest cell differentiation into melanocytes, and stimulates proliferation and differentiation of normal melanocytes. (ox.ac.uk)
  • Arkhammar, P, Nilsson, T & Berggren, PO 1986, ' Stimulation of insulin release by the phorbol ester 12-O-tetradecanoylphorbol 13-acetate in the clonal cell line RINm5F despite a lowering of the free cytoplasmic Ca 2+ concentration ', BBA - Molecular Cell Research , vol. 887, no. 2, pp. 236-241. (elsevier.com)
  • 12-O-tetradecanoylphorbol-13-acetate affects many cell actions and may cause tumor cells to die. (cancer.gov)
  • TPA (12-O-Tetradecanoylphorbol-13-acetate), a potent PKC activator, induces mS100a7a15 expression. (wikipedia.org)
  • 12-O-tetradecanoylphorbol-13-acetate (TPA) has been shown to induce differentiation and/or apoptosis in multiple cell lines and primary cells. (elsevier.com)
  • Recent studies have shown that topical application of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) to murine skin results in increased expression of the highly inflammatory cytokine interleukin (IL)-1 alpha in the epidermis. (nih.gov)
  • Treating animals with 3-Methylcholantrene (3MCA) and 7,12-Dimethylbenz(a) anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA), two potent carcinogens, confirmed Spalax high resistance to chemically induced cancers. (nih.gov)
  • 12-O-Tetradecanoylphorbol-13-acetate (TPA), a component of croton oil, is a potent mouse skin tumor promoter [ 1 , 2 ]. (hindawi.com)
  • Inhibition of 12-O-tetradecanoylphorbol-13-acetate and other skin tumor-promoter-caused induction of epidermal interleukin-1 alpha mRNA and protein. (nih.gov)
  • Garlisi, CG & Mastro, AM 1992, ' Characterization of the inhibition of interleukin 2 mRNA accumulation by 12-O-tetradecanoylphorbol-13-acetate in primary lymphocytes ', Lymphokine and Cytokine Research , vol. 11, no. 1, pp. 1-8. (elsevier.com)
  • Inhibition of 12-O-Tetradecanoylphorbol-13-Acetate-Induced Inflammatory Skin Edema and Ornithine Decarboxylase Activity by Theaflavin-3,3? (encognitive.com)
  • Determine the maximum tolerated dose and dose limiting toxicity of 12-O-tetradecanoylphorbol-13-acetate (TPA) in patients with relapsed or refractory hematologic malignancies or bone marrow disorders. (knowcancer.com)
  • We have studied the effects of the tumor promoter 12-O-tetradecanoylphorbol-13- acetate (TPA) in the differentiation phenotype of mucus-secreting (HT-29 M6) and absorptive (HT-29 M3) cells. (biologists.org)
  • Enhancement of human papillomavirus type 18 gene expression in HeLa cells by 12-O-tetradecanoylphorbol-13-acetate, 3 beta,5 alpha-dihydroxycholestan-6-one, and cholesterol. (semanticscholar.org)
  • We found previously that bovine lymph node cells (LNC) incubated with 12- O-tetradecanoylphorbol-13-acetate (TPA) for 18 h proliferate only to a limited degree on subsequent stimulation with concanavalin A (Con A), or with the comitogenic combination of Con A plus TPA. (elsevier.com)
  • Using oligonucleotide microarray, we have identified and cloned a novel gene that was induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in CD18 pancreatic cancer cells. (nus.edu.sg)
  • Stimulation of melanogenesis by tetradecanoylphorbol 13-acetate (TPA) in mouse melanocytes and neural crest cells. (ox.ac.uk)
  • Previous results have established that 12-O-tetradecanoylphorbol-13-acetate (TPA)-type tumor promoters can alter the properties of the epidermal growth factor (EGF) receptor through activation of protein kinase C. In order to determine whether other, non-TPA-type tumor promoters might similarly influence growth-mediating receptors, we investigated the effect of palytoxin on EGF binding in Swiss 3T3 fibroblasts and human epidermal carcinoma (A431) cells. (umn.edu)
  • This experiment focused on the expressional pattern of keratin 10 (K10 normal differentiation marker), and keratin 8 & 13 (K8 & K13 pathologic differentiation marker) together with their cellular localization after treating HaCaT cells with 12-Otetradecanoylphorbol 13-acetate (TPA). (bvsalud.org)
  • To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-0-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay . (bvsalud.org)
  • The method has been utilized to examine the stimulation of phosphatidylcholine breakdown in quiescent Swiss 3T3 cells in response to bombesin and 12-O-tetradecanoylphorbol 13-acetate (TPA). (biochemj.org)
  • We further investigated the preventive effect of DSW on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced invasive/metastatic tumor features in non-invasive MCF-7 cells. (spandidos-publications.com)
  • Basal and epidermal growth factor-induced ERK1/2 phosphorylation was inhibited in several cell lines as well as 12- O -tetradecanoylphorbol-13-acetate-induced ERK1/2 phosphorylation in isolated peripheral blood mononuclear cells. (aacrjournals.org)
  • Herein we demonstrate that vanillin reduced 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced MMP-9 gelatinolytic activity and suppressed cell invasion through the down-regulation of MMP-9 gene transcription in HepG2 cells. (aspetjournals.org)
  • Here, treatment of the skin with the carcinogen 7,12-dimethylbenz-[ α ]-anthracene (DMBA) and the tumour promoter 12- O -tetradecanoylphorbol-13-acetate (TPA) results in the formation of benign papillomas (PAPs) and malignant tumours (SCCs). (nature.com)
  • In the present study, we found that the topical application of xanthorrhizol before 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment significantly inhibits TPA-induced mouse ear edema and TPA-induced tumor promotion in 7,12-dimethylbenz[a]anthracene (DMBA)-initiated ICR mouse skin. (elsevier.com)
  • Carcinogenesis was initiated with 7,12-dimethylbenz( a )anthracene and promoted with 12- O -tetradecanoylphorbol-13-acetate (TPA). (aacrjournals.org)
  • 12-O-tetradecanoylphorbol-13-acetate TPA can stimulate acute and chronic inflammation and tumor promotion in skin. (duhnnae.com)
  • Treatment with anti-IL-12/23p40 or anti-IL-23p19 Abs greatly inhibited 12- O -tetradecanoylphorbol-13-acetate-induced epidermal hyperplasia in the ears of K5.Stat3C mice, whereas the inhibitory effect of an anti-IL-17A Ab was relatively less prominent. (jimmunol.org)
  • Identification, characterization and expression analysis of a novel TPA (12-0-Tetradecanoylphorbol-13-Acetate) induced gene. (nus.edu.sg)
  • Effects of electroporation on vimentin gene expression were compared at the cellular and chromatin level to those caused by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). (mpg.de)
  • We first determined the effect of topical application of GUG to mice against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced conventional markers and other novel markers of skin tumor promotion. (isharonline.org)
  • The papilloma response in mice, receiving pretreatments with 2 μgof 12-O-tetradecanoylphorbol-13-acetate (TPA) either 3 days, 1, 2, 3 or 5 weeks before initiation, was similar to that seen when TPA was given after initiation during stage I of promotion followed by stage II of promotion with mezerein (4-5 papillomas per mouse in allgroups). (uthscsa.edu)
  • Crossing IL-17A-deficient mice with K5.Stat3C mice resulted in partial attenuation of 12- O -tetradecanoylphorbol-13-acetate-induced lesions, which were further attenuated by anti-IL-12/23p40 Ab treatment. (jimmunol.org)
  • For example, K5.Stat3C transgenic mice, in which Stat3 is constitutively expressed in keratinocytes, developed psoriasiform lesions following wounding stimuli or topical treatment with the tumor promoter 12- O -tetradecanoylphorbol-13-acetate (TPA). (jimmunol.org)
  • Outbred CD-1 and outbred Sencar mice received a single topical application of the hydrocarbons followed by twice weekly applications of the tumor promoter 12-O-tetradecanoylphorbol 13-acetate for 16-26 weeks. (curehunter.com)
  • These compounds elicited significant in vivo anti-allergic and anti-inflammatory effects, suppressing an immunoglobulin E (IgE)-induced passive cutaneous anaphylactic reaction in mice and a 12-O-tetradecanoylphorbol-13-acetate-induced inflammatory mouse ear edema, respectively. (spandidos-publications.com)
  • Etiology studies indicate that chemical carcinogen promoters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA), are important factors causing NPC development. (elsevier.com)
  • The artificial in vitro activation of CD4+ T lymphocytes by a combination of 12-O-tetradecanoylphorbol-13-acetate and ionomycin, the so-called T/I model, led to an inducible production of cytokines, such as interferon-γ, tumor necrosis factor-α, and interleukin-2. (elsevier.com)
  • 12-O-Tetradecanoylphorbo-13-acetate (TPA) modulates DNA synthesis in bovine lymph node lymphocytes in culture. (elsevier.com)
  • Mastro, AM & Pepin, KG 1980, ' Suppression of Lectin-Stimulated DNA Synthesis in Bovine Lymphocytes by the Tumor Promoter 12-O-Tetradecanoylphorbol-13-acetate ', Cancer Research , vol. 40, no. 9, pp. 3307-3312. (elsevier.com)
  • Maximal expression of this phenotype required 72 h preactivation with phorbol myristate acetate and expression was abolished using the protein kinase C inhibitor staurosporine. (biomedsearch.com)
  • Apigenin is a nonmutagenic bioflavonoid that has been shown to be an inhibitor of mouse skin carcinogenesis induced by the two-stage regimen of initiation and promotion with dimethylbenzanthracene (DMBA) and 12-O-tetradecanoylphorbol-13-acetate (TPA). (nih.gov)
  • The painting of mouse dorsal skin with 1 2-0-tetradecanoylphorbol-l3-acetate (TPA) (0.2-2.5 nrTiol/mouse) induced a dose-related increase in vascular permeability, which was determined by pontamine sky blue exudation mto the skin 5 hr after the TPA treatment. (elsevier.com)
  • Since glycyrrhetinic acid was proved to suppress tumor promoter effects, several oleanane-type triterpenes which were chemically derived from oleanolic acid and hederagenin were tested in vitro and in vivo against the action of tumor promoter, 12- O -tetradecanoylphorbol 13-acetate. (aacrjournals.org)
  • abstract = "The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the regulation of the estrogen receptor (ER) was investigated in this study. (elsevier.com)
  • The effects of tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), cAMP analogue dibutyryl cAMP (DB) and histamine were studied on arginine decarboxylase activity, polyamine content and growth rate of Trypanosoma cruzi, RA strain epimastigotes. (brillonline.com)
  • The effects of 12-O-tetradecanoylphorbol 13-acetate (TPA) on the handling of Ca 2+ and insulin release were investigated in the clonal insulin-producing cell line RINm5F. (elsevier.com)
  • A tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), was found to increase the level of HPV18 transcripts in an HPV18-harboring cervical cancer cell line, HeLa. (semanticscholar.org)
  • Ultrastructural studies on cell fusion induced by Epstein-Barr virus or N-butyrate and 12-O-tetradecanoylphorbol-13-acetate. (uni-regensburg.de)
  • 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced increase in depressed white blood cell counts in patients treated with cytotoxic cancer chemotherapeutic drugs. (lclabs.com)
  • Patients receive 12-O-tetradecanoylphorbol-13-acetate (TPA) IV over 1 hour on days 1 and 8 followed by 2 weeks of rest. (knowcancer.com)
  • Effect of intravenous infusions of 12-O-tetradecanoylphorbol-13-acetate (TPA) in patients with myelocytic leukemia: preliminary studies on therapeutic efficacy and toxicity. (lclabs.com)