Drug Resistance, Microbial
Microbial Sensitivity Tests
Drug Resistance, Bacterial
DNA Transposable Elements
Drug Resistance, Multiple, Bacterial
Molecular Sequence Data
Electrophoresis, Gel, Pulsed-Field
Disk Diffusion Antimicrobial Tests
Drug Resistance, Multiple
Various forms of chemically induced liver injury and their detection by diagnostic procedures. (1/2398)A large number of chemical agents, administered for therapeutic or diagnostic purposes, can produce various types of hepatic injury by several mechanisms. Some agents are intrinsically hepatotoxic, and others produce hepatic injury only in the rare, uniquely susceptible individual. Idiosyncrasy of the host is the mechanism for most types of drug-induced hepatic injury. It may reflect allergy to the drug or a metabolic aberation of the host permitting the accumulation of hepatotoxic metabolites. The syndromes of hepatic disease produced by drugs have been classified hepatocellular, hepatocanalicular, mixed and canalicular. Measurement of serum enzyme activities has provided a powerful tool for studies of hepatotoxicity. Their measurement requires awareness of relative specificity, knowledge of the mechanisms involved, and knowledge of the relationship between known hepatotoxic states and elevated enzyme activities. (+info)
Assessment of hepatotoxic potential. (2/2398)Philosophic concepts and pragmatic approaches toward improved understanding of the effect of drugs in the hepatocyte are reviewed. No set pattern of studies is advocated but rather observations are encouraged within the framework of studies that provide for varied exposure of the hepatocyte. Clinical usage should be imitated to provide earliest possible indications of toxicity in man. The need for definitive characterization through utilization of appropriate methodology derived from cross-fertilization of related disciplines is stressed. Both minimal and maximal dose effects should be established. Selected use of electron microscopy has become essential for characterizing responses of the liver to injury. The advantages of the toluidine blue-stained Epon "thick" sections are emphasized. Such observations are used to implement the utility of serial biopsies from the beagle dog prior to and during long-term study of potential hepatic injury. Examples of the critical effects of drug concentration within the hepatocyte are presented. (+info)
Endemic tropical sprue in Rhodesia. (3/2398)The existence of tropical sprue in Africa is controversial. In this paper we present 31 cases seen in Rhodesia over a 15 month period. They have the clinical features, small intestinal morphology, malabsorption pattern, and treatment response of tropical sprue. Other causes of malabsorption, and primary malnutrition, have been excluded. The severity of the clinical state and intestinal malabsorption distinguish these patients from those we have described with tropical enteropathy. The previous work on tropical sprue in Africa is reviewed and it is apparent that, when it has been adequately looked for, it has been found. It is clear that the question of tropical sprue in Africa must be re-examined and that it existence may have hitherto been concealed by the assumption that primary malnutrition is responsible for the high prevalence of deficiency states. (+info)
In vivo chaperone activity of heat shock protein 70 and thermotolerance. (4/2398)Heat shock protein 70 (Hsp70) is thought to play a critical role in the thermotolerance of mammalian cells, presumably due to its chaperone activity. We examined the chaperone activity and cellular heat resistance of a clonal cell line in which overexpression of Hsp70 was transiently induced by means of the tetracycline-regulated gene expression system. This single-cell-line approach circumvents problems associated with clonal variation and indirect effects resulting from constitutive overexpression of Hsp70. The in vivo chaperone function of Hsp70 was quantitatively investigated by using firefly luciferase as a reporter protein. Chaperone activity was found to strictly correlate to the level of Hsp70 expression. In addition, we observed an Hsp70 concentration dependent increase in the cellular heat resistance. In order to study the contribution of the Hsp70 chaperone activity, heat resistance of cells that expressed tetracycline-regulated Hsp70 was compared to thermotolerant cells expressing the same level of Hsp70 plus all of the other heat shock proteins. Overexpression of Hsp70 alone was sufficient to induce a similar recovery of cytoplasmic luciferase activity, as does expression of all Hsps in thermotolerant cells. However, when the luciferase reporter protein was directed to the nucleus, expression of Hsp70 alone was not sufficient to yield the level of recovery observed in thermotolerant cells. In addition, cells expressing the same level of Hsp70 found in heat-induced thermotolerant cells containing additional Hsps showed increased resistance to thermal killing but were more sensitive than thermotolerant cells. These results suggest that the inducible form of Hsp70 contributes to the stress-tolerant state by increasing the chaperone activity in the cytoplasm. However, its expression alone is apparently insufficient for protection of other subcellular compartments to yield clonal heat resistance to the level observed in thermotolerant cells. (+info)
Inducible long-term gene expression in brain with adeno-associated virus gene transfer. (5/2398)Recombinant adeno-associated virus (rAAV) vectors hold promise for treating a number of neurological disorders due to the ability to deliver long-term gene expression without toxicity or immune response. Critical to these endeavors will be controlled expression of the therapeutic gene in target cells. We have constructed and tested a dual cassette rAAV vector carrying a reporter gene under the control of the tetracycline-responsive system and the tetracycline transactivator. Transduction in vitro resulted in stable expression from the vector that can be suppressed 20-fold by tetracycline treatment. In vivo experiments, carried out to 6 weeks, demonstrated that vector-transduced expression is sustained until doxycycline administration upon which reporter gene expression is reduced. Moreover, the suppression of vector-driven expression can be reversed by removal of the drug. These studies demonstrate long-term regulated gene expression from rAAV vectors. This system will provide a valuable approach for controlling vector gene expression both in vitro and in vivo. (+info)
Transcriptional regulation and induction of apoptosis: implications for the use of monomeric p53 variants in gene therapy. (6/2398)The p53 tumour suppressor protein is a transcriptional activator, which can induce cell cycle arrest and apoptosis. p53 Gene mutations occur in more than 50% of all human tumours. Reintroduction of wild-type p53 but also of oligomerisation-independent p53 variants into tumour cells by gene transfer methods has been considered. We have investigated the biological properties of two carboxy-terminal deletion mutants of p53, p53 delta 300 (comprising amino acids 1-300) and p53 delta 326 (amino acids 1-326), to evaluate their potential deployment in gene therapy. Transactivation was measured in transiently transfected HeLa and SKBR3 cells. Both monomeric variants showed reduced activities compared with wild-type p53. Individual promoters were differently affected. In contrast to wild-type p53, monomeric variants were not able to induce apoptosis. We also provided wild-type p53 and p53 delta 326 with tetracycline-regulated promoters and stably introduced these constructs into Saos2 and SKBR3 cells. Upon induction, wild-type p53 expressing cells, but not p53 delta 326 expressing cells underwent apoptosis. Consistently, only wild-type p53 expressing cells accumulated p21/waf1/cip1 mRNA and protein and showed increased bax, Gadd45 and mdm2 mRNA. Neither wild-type p53 nor p53 delta 326 repressed the transcription of the IGF-1R gene in these cell lines. We conclude that the transactivation potential of monomeric, carboxy-terminally truncated p53 is not sufficient to cause induction of the endogenous target genes which trigger apoptosis. (+info)
The stability and fate of a spliced intron from vertebrate cells. (7/2398)Introns constitute most of the length of typical pre-mRNAs in vertebrate cells. Thus, the turnover rate of introns may significantly influence the availability of ribonucleotides and splicing factors for further rounds of transcription and RNA splicing, respectively. Given the importance of intron turnover, it is surprising that there have been no reports on the half-life of introns from higher eukaryotic cells. Here, we determined the stability of IVS1Cbeta1, the first intron from the constant region of the mouse T-cell receptor-beta, (TCR-beta) gene. Using a tetracycline (tet)-regulated promoter, we demonstrate that spliced IVS1Cbeta1 and its pre-mRNA had half-lives of 6.0+/-1.4 min and 3.7+/-1.0 min, respectively. We also examined the half-lives of these transcripts by using actinomycin D (Act.D). Act.D significantly stabilized IVS1Cbeta1 and its pre-mRNA, suggesting that Act.D not only blocks transcription but exerts rapid and direct posttranscriptional effects in the nucleus. We observed that in vivo spliced IVS1Cbeta1 accumulated predominantly as lariat molecules that use a consensus branchpoint nucleotide. The accumulation of IVS1Cbeta1 as a lariat did not result from an intrinsic inability to be debranched, as it could be debranched in vitro, albeit somewhat less efficiently than an adenovirus intron. Subcellular-fractionation and sucrose-gradient analyses showed that most spliced IVS1Cbeta1 lariats cofractionated with pre-mRNA, but not always with mRNA in the nucleus. Some IVS1Cbeta1 also appeared to be selectively exported to the cytoplasm, whereas TCR-beta pre-mRNA remained in the nucleus. This study constitutes the first detailed analysis of the stability and fate of a spliced nuclear intron in vivo. (+info)
Functional importance and local environments of the cysteines in the tetracycline resistance protein encoded by plasmid pBR322. (8/2398)The properties of the cysteines in the pBR322-encoded tetracycline resistance protein have been examined. Cysteines are important but not essential for tetracycline transport activity. None of the cysteines reacted with biotin maleimide, suggesting that they are shielded from the aqueous phase or reside in a negatively charged local environment. (+info)
There are several types of acne, including:
1. Comedonal acne: characterized by blackheads and whiteheads.
2. Inflammatory acne: characterized by papules, pustules, and nodules.
3. Cystic acne: characterized by large, painful cysts that can cause scarring.
4. Acne rosacea: a type of acne that occurs in adults, characterized by redness, flushing, and telangiectasias (small blood vessels).
There are several treatment options for acne vulgaris, including:
1. Topical treatments: such as benzoyl peroxide, salicylic acid, and sulfur.
2. Oral antibiotics: such as doxycycline and minocycline.
3. Retinoids: derived from vitamin A, used to unclog pores and reduce inflammation.
4. Hormonal therapies: such as birth control pills, used to regulate hormones that can contribute to acne.
5. Isotretinoin: a powerful oral medication used for severe cases of cystic acne that have not responded to other treatments.
6. Laser and light therapy: such as blue light therapy and photodynamic therapy, used to reduce inflammation and kill bacteria.
7. Lifestyle modifications: such as using non-comedogenic products, wearing sunscreen, and avoiding picking or popping pimples.
It is important to note that acne can be a persistent condition, and it may take time and experimentation to find the right treatment approach. It's best to consult with a dermatologist for personalized advice on treating acne vulgaris.
Symptoms of gonorrhea in men include:
* A burning sensation when urinating
* Discharge from the penis
* Painful or swollen testicles
* Painful urination
Symptoms of gonorrhea in women include:
* Increased vaginal discharge
* Painful urination
* Painful intercourse
* Abnormal vaginal bleeding
Gonorrhea can be diagnosed through a physical exam and laboratory tests, such as a urine test or a swab of the affected area. It is typically treated with antibiotics.
If left untreated, gonorrhea can cause serious complications, including:
* Pelvic inflammatory disease (PID) in women
* Epididymitis (inflammation of the tube that carries sperm) in men
* Chronic pain
* Increased risk of HIV transmission
Gonorrhea is a reportable disease, meaning that healthcare providers are required by law to report cases to public health authorities. This helps to track and prevent the spread of the infection.
Prevention methods for gonorrhea include:
* Safe sex practices, such as using condoms or dental dams
* Avoiding sexual contact with someone who has gonorrhea
* Getting regularly tested for STIs
* Using pre-exposure prophylaxis (PrEP) for HIV prevention
It is important to note that gonorrhea can be asymptomatic, meaning that individuals may not experience any symptoms even if they have the infection. Therefore, regular testing is important for early detection and treatment.
The symptoms of cholera include:
1. Diarrhea: Cholera causes profuse, watery diarrhea that can last for several days.
2. Dehydration: The loss of fluids and electrolytes due to diarrhea can lead to severe dehydration, which can be life-threatening if not treated promptly.
3. Nausea and vomiting: Cholera patients may experience nausea and vomiting, especially in the early stages of the disease.
4. Abdominal cramps: The abdomen may become tender and painful due to the inflammation caused by the bacteria.
5. Low-grade fever: Some patients with cholera may experience a mild fever, typically less than 102°F (39°C).
Cholera is spread through the fecal-oral route, which means that it is transmitted when someone ingests food or water contaminated with the bacteria. The disease can also be spread by direct contact with infected fecal matter, such as through poor hygiene practices or inadequate waste disposal.
There are several ways to diagnose cholera, including:
1. Stool test: A stool sample can be tested for the presence of Vibrio cholerae using a microscope or a rapid diagnostic test (RDT).
2. Blood test: A blood test can detect the presence of antibodies against Vibrio cholerae, which can indicate that the patient has been infected with the bacteria.
3. Physical examination: A healthcare provider may perform a physical examination to look for signs of dehydration and other symptoms of cholera.
Treatment of cholera typically involves replacing lost fluids and electrolytes through oral rehydration therapy (ORT) or intravenous fluids. Antibiotics may also be given to shorten the duration of diarrhea and reduce the risk of complications. In severe cases, hospitalization may be necessary to provide more intensive treatment.
Prevention of cholera involves maintaining good hygiene practices, such as washing hands with soap and water, and avoiding consumption of contaminated food and water. Vaccines are also available to protect against cholera, particularly for people living in areas where the disease is common.
In conclusion, cholera is a highly infectious disease that can cause severe dehydration and even death if left untreated. Early diagnosis and treatment are critical to preventing complications and reducing the risk of transmission. Prevention measures such as vaccination and good hygiene practices can also help control the spread of the disease.
In women, urethritis is more common than in men due to the shorter length of their urethra and their closer proximity to the anus, which can increase the risk of bacterial infection. In addition, certain sexually transmitted infections (STIs), such as chlamydia and gonorrhea, can cause urethritis in both men and women.
If you suspect that you or a partner may have urethritis, it is important to seek medical attention as soon as possible. Untreated urethritis can lead to complications such as recurrent infections, infertility, and an increased risk of certain types of cancer. A healthcare provider can diagnose urethritis by performing a physical examination, taking a urine sample for testing, and possibly performing additional tests such as a pelvic exam or ultrasound to rule out other conditions. With prompt and appropriate treatment, however, most cases of urethritis can be effectively managed and cured.
Tetracycline-controlled transcriptional activation
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George J. Armelagos
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Idiopathic intracranial hypertension
List of allergens
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Tetracycline-Resistant Neisseria gonorrhoeae -- Georgia, Pennsylvania, New Hampshire
TETRACYCLINE HYDROCHLORIDECAPSULES, USP
Tetracycline class drugs
Tetracycline hydrochloride 10381-S
Tetracycline: MedlinePlus Drug Information
Dental and oral discolorations associated with minocycline and other tetracycline analogs
MedlinePlus - Search Results for: TETRACYCLINE
Tetracycline dosage for ear infection
International Standard for Tetracycline
Tetracycline - PubMed
Tetracycline - PubMed
TETRACYCLINE - Mar Vista Animal Medical Center
Tetracycline - Drugs and Lactation Database (LactMed®) - NCBI Bookshelf
Tetracyclines - Infectious Diseases - Merck Manuals Professional Edition
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"Diversity of Tetracycline Resistance Among Bacterial Isolates from Swi" by Craig D. Adams, J Macauley et al.
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Tetracycline hydrochloride, yellow powder | VWR
tetracycline - NIH Director's Blog
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Tetracycline class drugs
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- An overview of Genetic Toxicology Mammalian Cell Mutagenicity study conclusions related to Tetracycline hydrochloride (64-75-5). (nih.gov)
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- All were resistant to tetracycline (MIC 16-32 ug/ml), doxycycline (MIC 8-24 ug/ml), and minocycline (MIC 12-32 ug/ml). (cdc.gov)
- It has been well acknowledged in recent literature that minocycline, a semisynthetic tetracycline derivative, causes discolorations in adult teeth and various other collagenous tissues. (nih.gov)
- This article presents the most common patterns of minocycline staining in addition to comparing the staining patterns of other tetracycline analogs in the permanent dentition. (nih.gov)
- Minocycline and other tetracycline analogs are well known for causing discoloration of developing teeth in children. (nih.gov)
- tell your doctor and pharmacist if you are allergic to tetracycline, minocycline, doxycycline, demeclocycline, any other medications, or any of the ingredients in the tetracycline capsule. (medlineplus.gov)
- Minocycline is in a class of medications called tetracycline antibiotics. (nih.gov)
- Tetracycline has since been supplanted, at least in small animal medicine, by minocycline and doxycycline , which have less side effects potential and more convenient dosing schedules. (marvistavet.com)
- More modern forms of tetracycline include doxycycline and minocycline which are much more commonly used and have similar indications. (nih.gov)
- Chronic therapy with tetracycline is effective in ameliorating acne, but because of their better absorption and tissue penetration, minocycline and doxycycline have largely replaced tetracycline for this indication. (nih.gov)
- Tetracycline is in a class of medications called tetracycline antibiotics. (medlineplus.gov)
- Antibiotics such as tetracycline will not work for colds, flu, or other viral infections. (medlineplus.gov)
- This syndrome is rarely seen currently as tetracycline is no longer available in parenteral form and the use of intravenous tetracyclines has been superseded by availability of safer, better tolerated and more effective broad spectrum antibiotics. (nih.gov)
- Take tetracycline exactly as directed. (medlineplus.gov)
- Take tetracycline 2 hours before or 6 hours after antacids, calcium supplements, zinc products, and laxatives containing magnesium. (medlineplus.gov)
- Take tetracycline 2 hours before or 4 hours after iron preparations and vitamin products that contain iron. (medlineplus.gov)
- Take tetracycline 2 hours before or after zinc containing products. (medlineplus.gov)
- Take Tetracycline orally on an empty stomach at least 1 hour before or 2 hours after eating. (canadianpharm.org)
- To clear up your infection completely, take Tetracycline for the full course of treatment. (canadianpharm.org)
- Take Tetracycline at regular intervals. (cryptocoinpharma.com)
- Take Tetracycline exactly as directed by your doctor or according to the instructions on the label. (cryptocoinpharma.com)
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- For people who have been exposed to anthrax but do not have symptoms, 60 days of ciprofloxacin, a tetracycline (including doxycycline), or penicillin is given to reduce the risk or progression of disease due to inhaled anthrax. (medscape.com)
- When the dual transgenic mice are fed tetracycline or doxycycline, the transgene is activated in glomerular podocytes. (nih.gov)
Forms of tetracycline1
- High doses of several forms of tetracycline given intravenously have been associated with acute fatty liver that can be severe and result in liver failure and death. (nih.gov)
- Store Tetracycline at room temperature, between 68 and 77 degrees F (20 and 25 degrees C), in a tightly closed, light-resistant container. (canadianpharm.org)
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- Tetracycline was first approved for use in the United States in 1957 and was one of several oral tetracyclines used at that time (oxytetracycline, chlortetracycline), many of which are no longer available or are used in veterinary medicine only. (nih.gov)
- Since February 1985, CDC has identified 12 isolates of Neisseria gonorrhoeae that have high-level resistance to tetracycline (minimal inhibition concentration (MIC) 24-32 ug/ml) but are susceptible to penicillin. (cdc.gov)
- Therefore, tetracyclines should not be used for streptococcal disease unless the organisms have been demonstrated to be susceptible. (nih.gov)
- If the Kirby-Bauer method of disk susceptibility testing is used, a 30 mcg tetracycline disk should give a zone of at least 19 mm when tested against a tetracycline susceptible bacterial strain. (nih.gov)
- Tetracycline is an oral, broad-spectrum antibiotic used to treat mild-to-moderate infections due to susceptible microbial organisms. (nih.gov)
- Six patients were initially treated with oral tetracycline alone. (cdc.gov)
- Oral tetracycline use has been rarely and not very convincingly linked to acute hepatic injury. (nih.gov)
- Tetracycline is an oral, broad-spectrum antibiotic and semisynthetic derivative of Streptomyces actinobacteria. (nih.gov)
- Oral tetracycline has been associated with rare instances of acute liver injury, but the association with tetracycline use as opposed to other agents being taken has not always been very well shown. (nih.gov)
- Despite frequency of its use, oral tetracycline remains a very rare cause of liver injury. (nih.gov)
- digoxin, methotrexate, methoxyflurane, or oral anticoagulants (warfarin) because the risk of their side effects may be increased by Tetracycline. (canadianpharm.org)
- The drugs in the tetracycline class have closely similar antimicrobial spectra, and cross-resistance among them is common. (nih.gov)
- A tetracycline disk may be used to determine microbial susceptibility to drugs in the tetracycline class. (nih.gov)
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- The tetracycline antibiotic family provides broad anti-bacterial protection by inhibiting bacterial protein synthesis. (marvistavet.com)
- In other words, tetracycline binds to bacterial protein-synthesis structures but not to mammalian ones. (marvistavet.com)
- Another use would be the treatment of a feline condition known as a "Tetracycline Responsive Abscess" where draining abscesses are caused by "L-form" bacteria (a bacterial type that lacks a cell wall). (marvistavet.com)
- Hepatic injury associated with small bowel bacterial overgrowth in rats is prevented by metronidazole and tetracycline. (nih.gov)
- Diversity of Tetracycline Resistance Among Bacterial Isolates from Swi" by Craig D. Adams, J Macauley et al. (usu.edu)
- Tetracycline is a broad-spectrum polyketide antibiotic produced by the Streptomyces genus of Actinobacteria, indicated for use against many bacterial infections. (radjay.com)
- Tetracycline is a broad-spectrum polyketide antibiotic used to treat certain bacterial infections of the lungs, skin, and sexually transmitted diseases (STD). (cryptocoinpharma.com)
- Tetracycline exerts its bacteriostatic effect by passively diffuses through porin channels in the bacterial membrane and reversibly binding to the ribosome's 30S subunits, thereby preventing the binding of aminoacyl transfer RNA to messenger RNA-ribosome complex and inhibiting protein synthesis, thus arresting cell growth. (cryptocoinpharma.com)
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- Each capsule contains 250 mg of Tetracycline as its active ingredient. (cryptocoinpharma.com)
- Bismuth, metronidazole, and tetracycline is used along with other ulcer medications to treat duodenal ulcers. (nih.gov)
- Tetracycline is available in multiple generic forms as capsules or tablets of 250 and 500 mg and generally recommended in doses of 250 to 500 mg three to four times daily for 7 to 30 days. (nih.gov)
- High doses of intravenous tetracycline can induce fatty liver disease and may result in severe hepatic dysfunction, acute liver failure and death. (nih.gov)
- Tetracycline is an antibiotic, and in high doses can permanently stain teeth. (flaxdental.com)
- you should know that when tetracycline is used during pregnancy or in babies or children up to age 8, it can cause the teeth to become permanently stained. (medlineplus.gov)
- Antacids commonly contain calcium, which binds tetracycline in the GI tract. (marvistavet.com)
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Acute fatty liver1
- However, instances of acute fatty liver attributed to intravenous tetracycline have been reported in nonpregnant women and in men and even in children. (nih.gov)
- Silagra is a generic version of the brand name drug called Viagra does tetracycline cure std. (vivalaslearn.com)
- Tetracyclines are readily absorbed and are bound to plasma protein in varying degrees. (nih.gov)
- This list does not include all medicines that may interact with Tetracycline. (cryptocoinpharma.com)
- This was a 28-year-old homosexual male who had positive posttreatment cultures from both the rectum and pharynx after initial treatment with tetracycline for gonococcal infection at those sites. (cdc.gov)
- Currently, tetracycline is most frequently used for upper respiratory and skin and soft tissue infection and more than 2 million prescriptions are filled yearly. (nih.gov)
- Tetracycline is also sometimes used to treat Lyme disease and malaria, and to prevent plague and tularemia in people who have been exposed to plague or tularemia germs. (medlineplus.gov)
- Tetracycline is also active against infections with several rickettsial, spirochetal, chlamydial and mycoplasmas infections and are often used for therapy of nonspecific urethritis and several Rickettsia diseases, such as Rocky Mountain spotted fever and Lyme disease. (nih.gov)
- Long term use may induce actual urinary stones made of tetracycline (a rare but interesting complication). (marvistavet.com)
- When it comes to hard-to-remove stains, like those from tetracycline, the ballgame changes a little. (flaxdental.com)
- The management of patients with stains by tetracycline is complicated depending on the degree of stain that each patient presents when attending These situations may occur and the professional should be properly prepared to handle these cases. (bvsalud.org)
- Pharmacie En Ligne Andorre Cialis does tetracycline cure std . (vivalaslearn.com)
- Tetracycline may cause side effects. (medlineplus.gov)