Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
Methods for maintaining or growing CELLS in vitro.
Techniques used in studying bacteria.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Techniques used in microbiology.
Established cell cultures that have the potential to propagate indefinitely.
Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Elements of limited time intervals, contributing to particular results or situations.
A series of steps taken in order to conduct research.
Process of growing viruses in live animals, plants, or cultured cells.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Infections by bacteria, general or unspecified.
A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
Substances that reduce the growth or reproduction of BACTERIA.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A suborder of PRIMATES consisting of six families: CEBIDAE (some New World monkeys), ATELIDAE (some New World monkeys), CERCOPITHECIDAE (Old World monkeys), HYLOBATIDAE (gibbons and siamangs), CALLITRICHINAE (marmosets and tamarins), and HOMINIDAE (humans and great apes).
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
... in vitro research using human cell and tissue cultures; clinical studies; human autopsy examinations; computerised patient-drug ... mathematical models and computer simulations and non-invasive imaging techniques. Since the launch of the campaign, two major ...
Introduction to Cell and Tissue Culture: Theory and Technique. Plenum Press. New York City and London.. ... The decrease in viable cells after plating is due to "anchorage-dependence"--cells must attach to the bottom of the culture ... cells inoculated}}}}\times 100} The first method is more accurate. Cell growth in culture generally undergoes a decline after ... cells plated on day 0 × 100 {\displaystyle \mathrm {PE} ={\frac {\#\,{\text{cells on day 1}}}{\#\,{\text{cells plated on day 0 ...
Myopathies have been modeled with cell culture systems of muscle from healthy or diseased tissue biopsies. Another source of ... Research on skeletal muscle properties uses many techniques. Electrical muscle stimulation is used to determine force and ... Another group of cells, the myosatellite cells are found between the basement membrane and the sarcolemma of muscle fibers.[2] ... The fibres and muscles are surrounded by connective tissue layers called fasciae. Muscle fibres, or muscle cells, are formed ...
She obtained cells which possessed osteogenic characteristics from cultured tissue using chick limb bones which were separated ... Enzymatic digestion is one of the most advanced techniques for isolating bone cell populations and obtaining osteoblasts. Peck ... She obtained cells which possessed osteogenic characteristics from cultured tissue. ... Bone is a highly vascular tissue, and active formation of blood vessel cells, also from mesenchymal stem cells, is essential to ...
Burrows studied tissue culture. At Yale, Burrows also successfully established tissue cultures of embryonic chick cells. Upon ... Burrows and Carrel went on to expand the application of their tissue culture techniques to additional types of tissues ... One significant innovation was the method they developed for serial activation of tissues in culture. This technique ... Their batch of cultures lasted from 1912 to 1946. Because the tissue cultures far outlived their organisms, they were deemed " ...
Myopathies have been modeled with cell culture systems of muscle from healthy or diseased tissue biopsies. Another source of ... Research on skeletal muscle properties uses many techniques. Electrical muscle stimulation is used to determine force and ... Connective tissue is present in all muscles as fascia. Enclosing each muscle is a layer of connective tissue known as the ... Another group of cells, the myosatellite cells are found between the basement membrane and the sarcolemma of muscle fibers. ...
In this analogy the field is a tissue culture dish, the plates the cells growing on it. While this was a reasonable and ... Changes in the amounts of proteins synthesized by cells are measured using a variety of techniques such as the green ... In review, classical 20th century cell biology used cell lines grown in culture where the experiments were measured using very ... in cell-based systems uses living cells as tools in biological research to elucidate the workings of normal and diseased cells ...
Counting Actively Metabolizing Tissue Cultured Cells, Exp. Cell. Res., 1957, 13, 341-347. Masters R.W., Animal Cell Culture, ... Culture of Animal Cells, A Manual of Basic Technique, 5th edition, Wiley-Liss, 200- 201. Mohanta T.K., Patra J.K., Rath S.K., ... Masters R.W., Animal Cell Culture, Cytotoxicity and Viability Assays, Third Edition, 202-203.. ... Skehan P., Evaluation of Colorimetric Protein and Biomass Stains for Assaying Drug Effects Upon Human Tumor Cell Lines, Proc. ...
... cell cloning is an arduous task as these cells will not readily grow in standard media. A useful tissue culture technique used ... Cloning a cell means to derive a population of cells from a single cell. In the case of unicellular organisms such as bacteria ... ISBN 978-0-8053-4665-7. McFarland, Douglas (2000). "Preparation of pure cell cultures by cloning". Methods in Cell Science. 22 ... 24 January 2018). "Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer". Cell. 172 (4): 881-887.e7. doi:10.1016/j.cell. ...
The tumor was cultured in vivo, which became known as the Ehrlich cell. After 1948 Ehrlich cultures spread around research ... Culture techniques in large-scale, mice passage is less attractive, due to the contamination of the tumor with multifarious ... This made it useful for biochemical studies involving large amounts of tissues. It could also be maintained in vitro for more ... "Osmotic properties of ehrlich ascites tumor cells during the cell cycle". J Cell Physiol. 97 (3): 381-96. doi:10.1002/jcp. ...
Through tissue culture techniques a single tobacco cell was selected that contained the gene and a new plant grown from it. The ... Predieri S (2001). "Mutation induction and tissue culture in improving fruits". Plant Cell, Tissue and Organ Culture. 64 (2/3 ... Electroporation is used when the plant tissue does not contain cell walls. In this technique, "DNA enters the plant cells ... In the 1980s techniques were developed to introduce isolated chloroplasts back into a plant cell that had its cell wall removed ...
Through tissue culture techniques a single tobacco cell was selected that contained the gene and a new plant grown from it. The ... The range of plants that can be transformed has increased as tissue culture techniques have been developed for different ... To do this the cells undergoes a process called resolution, where during bacterial cell division one new cell receives the ... In the 1980s techniques were developed to introduce isolated chloroplasts back into a plant cell that had its cell wall removed ...
Simmons contributes to the fields of mechanobiology, stem cells, microfluidics and tissue engineering. Simmons reports being ... the lab uses in vitro co-culture systems to mechanically stimulate heart valve cells and investigate the phenotypic expression ... It tries to identify though microgenomics techniques, the cellular and molecular differences in diseased vs. healthy heart ... The goal is to stimulate cells in a controlled manner. The lab's microfluidic platforms models vascularized cell environments. ...
... studies mechanical and electrical energy in living cells and tissues Cell biology: studies cell-level organization and ... These can include cultured human or animal cells grown outside of the whole organism, small animals such as flies, worms, fish ... This approach emphasizes focus on a particular body or disease process as a whole and drawing upon the techniques of multiple ... tissues, and cells Pharmacology: studies effects of drugs on biological systems Physiology: studies how various body systems ...
Viruses, which only replicate in living cells, are studied in the laboratory in cell or tissue culture, and many animal ... They now involve the full range of techniques used in molecular biology, such as the omics. In contrast, studies conducted in ... in vivo tissue and organ sensitivities can be completely different or even inverse of those observed on cells cultured and ... in cell or tissue culture); subcellular components (e.g. mitochondria or ribosomes); cellular or subcellular extracts (e.g. ...
In cell culture, it has been used to study EGF receptor signaling[16] and trafficking.[17] Time domain FLIM (tdFLIM) has also ... Imaging technique based on fluorescence. Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the ... where light scattering by brain tissue is problematic for ratiometric imaging.[19] In neurons, FLIM imaging using pulsed ... Methods in Cell Biology. 81. pp. 495-524. doi:10.1016/S0091-679X(06)81024-1. ISBN 9780123740250. . PMID 17519182.. ...
The technique will leverage computer assisted design models, co-culture media which support muscle and fat cells as well as ... Cultured meat is produced using many of the same tissue engineering techniques traditionally used in regenerative medicine. The ... Cultured meat is meat produced by in vitro cell culture of animal cells, instead of from slaughtered animals. It is a form of ... the density of the cells on the scaffold means that not all cells have an interface with culture media, leading to cell death ...
... cultured in vitro". Plant Cell, Tissue and Organ Culture. 25 (2): 117-23. doi:10.1007/BF00042182 (inactive 10 January 2021).CS1 ... a technique employed by isolating and harvesting meristem cells from pieces of tissue. Auxins NAA, 2,4-D and IAA alone or ... 1995). "Calcium restriction induces cardenolide accumulation in cell suspension cultures of Digitalis thapsi L". Plant Cell ... manganese and lithium on growth and cardenolide content in cell suspension cultures of Digitalis thapsi L.". Plant Cell Reports ...
As an outgrowth of the latter topic, Loeb developed the cell culture technique as applied to both normal and abnormal tissues. ... he continued his work on tissue transplantation and cell culture, as well as research on endocrine disease. Loeb was known as a ... Loeb became interested in blood coagulation and the growth properties of malignant cells. ...
... establishing the methodology of tissue culture. Cell culture techniques were advanced significantly in the 1940s and 1950s to ... Cancer Cells in Culture Evolution of Cell Culture Surfaces Hypertext version of the Cell Line Data Base Microcarrier Cell ... Plant cell lines Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell) Other species cell ... Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and ...
"The research for the SV40 by means of tissue culture technique". Nippon Rinsho. 21 (6): 1201-1219. Shimizu B (1993). Seno K, ... ISBN 978-4-320-05386-1. "Main Types of Cell Culture". Fundamental Techniques in Cell Culture: a Laboratory Handbook. Retrieved ... Vero cells are a lineage of cells used in cell cultures. The 'Vero' lineage was isolated from kidney epithelial cells extracted ... F6B1.1) HeLa cells Immortalised cell line History and Characterization of the Vero Cell Line -- A Report prepared by CDR ...
... such as acinar structures in healthy and cancerous breast tissue models. These techniques have been applied to for in vitro ... Cell culture Cell lines Cell culture assay Hydrogel Madin-Darby Canine Kidney cell line Microphysiometry Fey S, Wrzesinski K ( ... cell-cell/cell-matrix interaction, and/or cell migration, but the available assays are not optimized for 3D cell culturing. ... In living tissue, cells exist in 3D microenvironments with intricate cell-cell and cell-matrix interactions and complex ...
Mesenchymal stem cells, but not red blood cells or haematopoetic progenitors, are adherent to tissue culture plastic within 24 ... The majority of modern culture techniques still take a colony-forming unit-fibroblasts (CFU-F) approach, where raw unpurified ... Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside ... May 2012). "Mesenchymal-stem-cell-induced immunoregulation involves FAS-ligand-/FAS-mediated T cell apoptosis". Cell Stem Cell ...
Having learned about tissue culture techniques from Alexis Carrel, Strangeways took great interest in the new field, including ... St Bartholomew's Hospital so that the laboratory could focus on then-newly developing technologies in tissue culture and cell ... In the 1920s and 30s, the laboratory was the only British institution focused specifically on tissue culture technique, the ... Wilson, D (August 2005). "The Early History of Tissue Culture in Britain: The Interwar Years". Social History of Medicine. 18 ( ...
In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed ... Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding ... and the pieces are placed in a cell culture dish containing growth media. Over time, progenitor cells migrate out of the tissue ... The tissue must be placed and harvested in an aseptic environment such as sterile laminar flow tissue culture hood. The samples ...
Electron microscopy is a common method that uses the immunolabeling technique to view tagged tissues or cells. The electron ... Moreover, cell cultures of human pituitary adenomas were viewed by light microscopy and immunocytochemistry, where these cells ... This is an example of how a immunolabeled cell culture of pituitary adenoma cells that were viewed via light microscopy and by ... For instance, a study was conducted to view the morphology and the production of hormones in pituitary adenoma cell cultures ...
The experimental units can for example be cell-cultures, tissues, organs or living animals. The biological response may be ... Statistical techniques such as optimization by Maximum Likelihood can be used to calculate estimates of the parameters. Of ... ISBN 0-02-844640-2 Govindarajulu, Z. (2001). Statistical Techniques in Bioassay, 2nd revised and enlarged edition, Karger, New ... usually by interpolation techniques, and in many cases to express the potency/activity of the test preparation(s) relative to a ...
... which can deliver oligos into tissues of later embryonic stages. Common techniques for delivery into cultured cells include the ... enter cells when administered systemically in adult animals or in tissue cultures. In eukaryotic organisms, pre-mRNA is ... Techniques for targeting Morpholinos to RNAs and delivering Morpholinos into cells have recently been reviewed in a journal ... the minor spliceosome acts outside the nucleus and controls cell proliferation". Cell. 131 (4): 718-29. doi:10.1016/j.cell. ...
... techniques to discover pharmaceutically-important compounds produced from local isolates and to develop plant cell culture ... and tissue culture technology for the identification and mass production of identified novel compounds. Continuing efforts are ... It is a member of the World Federation of Culture Collections and serves as the headquarters of the Philippine Network of ... PNCM has active linkages with culture collection laboratories here and abroad. CASL handles routine chemical analysis of food, ...
... since she had been trained in both cell culture and tissue staining techniques in the United States. Turpin agreed to provide ... Gautier was also working half-time as a technician in a laboratory for cell culture to obtain in-vitro cultures of fibroblast ... At Harvard, one of the tasks of her internship was to be trained as a laboratory technician working on cell culture. Besides ... At the time, Turpin favored the hypothesis of a chromosomal origin of trisomy but there was no laboratory for cell culture in ...
... but they are fragile and difficult to keep alive in cell ... Adult neural stem cells are thought of as having a lot of ... New 3D Tissue Printing Technique Being Adapted to Culture Adult Neural Stem Cells. July 24th, 2014 Medgadget Editors News ... Nanostimulators Activate Damaged Tissue to Heal Itself. Artificial Red Blood Cells to Carry Oxygen, Drugs, and Work as ... The researchers created a 3-D tissue printer that lays down cells, hydrogels, and other components layer by layer. They also ...
Course Title: Perform tissue and cell culture techniques. Part B: Course Detail. Teaching Period: Term2 2009 ... Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture Select specified culture ... Introduction to plant tissue culture and plant physiology. 2. Tissue Culture environment. Practical One: Brassica Rapa seeds ... Monitor growth of tissue and cell lines and products Detect contamination and troubleshoot materials, equipment and techniques ...
Cell cultures are utilized in cytogenetic, biochemical, and ... for Mammalian Cell Tissue Culture UNIT 1.1 Tissue culture ... technology has found wide application in the field of cell biology. ... Essential Techniques of Cancer Cell Culture Basic Techniques of Cell Culture 17 2 Essential Techniques of Cancer Cell Culture ... 1 Basic Techniques for Mammalian Cell Tissue Culture UNIT 1.1 Tissue culture technology has found wide application in the field ...
Techniques in the (bio)chemical labs Tissue culture *Two separate TC labs for ML-I and ML-II tissue culture ...
Researchers recreate pancreatic tissue in vitro using 3D cell culture technique. *Download PDF Copy ... Posted in: Cell Biology , Life Sciences News. Tags: Cancer, Cancer Treatment, Cell, Cell Culture, Drugs, Fibrosis, in vitro, ... cell culture technique, they devised a method to mix pancreatic cancer cells together with fibrotic components to generate ... Considering this, the research team came up with a simple strategy: mix and culture pancreatic cancer cells and fibroblasts at ...
Cultured Cells for Cartilage Tissue Engineering. * Front Matter Pages 163-163 PDF. ... Injectable Autologous Bone-Marrow-Derived Mesenchymal Stem Cells for Cartilage Repair: The Singapore Technique ... Surgical Techniques in Cartilage Repair Surgery: Osteochondral Autograft Transfer (OATS, Mosaicplasty) László Hangody, Ágnes ... This book fills a gap in surgical techniques for cartilage repair. All of the surgical chapters are logically organised, ...
Cell and tissue culture techniques for cereal crop improvement : proceedings of a workshop [Beijing, 1981]. Open All Close All ... "Cell and tissue culture techniques for cereal crop improvement : proceedings of a workshop [Beijing, 1981]"@en ... "Cell and tissue culture techniques for cereal crop improvement : proceedings of a workshop sponsored by the Institute of ... "Cell and tissue culture techniques for cereal crop improvement proceedings of a workshop"@en ...
Fluorescence microscopy techniques. *Gene expression. *Gene expression profiling - tissue level. *Histology. *Human cell ... 4)Musculoskeletal engineering: 1) Mechanisms for directing cell differentiation in 3D models of tissue constructs 2) Cell ... Less is more: new biomimetic approach to control spatial and temporal cell loading for tissue engineering. Journal article ... Less is more: New biomimetic approach to control spatial and temporal cell loading for tissue engineering Journal article ...
Purchase Cells and Tissues in Culture Methods, Biology and Physiology - 1st Edition. Print Book & E-Book. ISBN 9781483231464, ... Techniques of Organ, Tissue and Free Cell Culture References 9. The Nutrition and Metabolism of Plant Tissue and Organ Cultures ... Sources of Cells and Tissues V. Growth by Cell Division in Tissue Cultures VI. Aggregation of Dissociated Cells VII. Culture of ... 4. Cell, Tissue and Organ Cultures in Virus Research. I. Introduction II. Isolation of Viruses in Tissue Culture III. ...
staining techniques. Medical[edit]. *Cell and tissue culture. *Patient sample handling techniques ... Although books exist to teach these techniques, experienced lab workers have special tricks and techniques that they do not ... Biology Laboratory Techniques. From Wikibooks, open books for an open world. The latest reviewed version was checked on 17 ... Even so, there are some basic techniques that all beginning lab students should understand. ...
Biochemical Microbiological Hematological Cell/ tissue culture * 23. Learning objective ,ul,,li,The meaning of pathology and ... li,,/ul,2. How to study pathology? 4. The general main causes of disease 3. Techniques of Pathology ... Sample comparison Structure Normal squamous cell VS abnormal squamous cell Function of organ, tissue, cell Normal blood sugar ... ul,,li,Cell ,/li,,/ul,,ul,,li,Tissue ,/li,,/ul,,ul,,li,Organ ,/li,,/ul,Environmental factors Genetic factors Change with ...
... a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques f ... genes phorbol ester regulation reproducible techniques tissue Editors and affiliations. *Joanna Picot*1 ... Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach ... authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types ...
Cell and tissue culture techniques,. *Production and purification of agronomic relevant proteins in different expression ... Current topics in cell biology and biochemistry of plants (e.g. chromatin, signalling, cell polarity, cell-cell communication ... and cell fusion);. *Current topics in plant biotechnology (e.g. transformation methods and tissue culture, breeding traits and ... Molecular Cell and Developmental Biology of Plants. Prof. Dr. Thomas Dresselhaus. Plant Biotechnology. Prof. Dr. Thomas ...
Tissue-resident mast cells may act as IL-17A-loaded sentinel cells, which release IL-17A to amplify tissue inflammation. ... IL-17A-positive mast cells are present across SpA target tissues and correlate inversely with inflammation, indicating that ... Cell Culture Techniques * Humans * Inflammation * Interleukin-17 / metabolism* * Mast Cells / metabolism* * Spondylarthritis / ... Tissue-resident mast cells may act as IL-17A-loaded sentinel cells, which release IL-17A to amplify tissue inflammation. ...
... classification of soft-tissue sarcomas (STS) has improved with cytogenetic analyses, but their clinical behavior is still not ... Cell Culture Techniques * Cell Line, Tumor * Disease-Free Survival * Female * Follow-Up Studies ... Median mRNA values of all genes studied were higher in tumors than in paired normal tissues. In agreement with data on STS cell ... Real-time PCR was performed on human STS cell lines and tissues from highly malignant STS, including leiomyosarcomas and ...
Embryology and Cell Biology by Christine C. Stichel-Gunkel. Buy a discounted Paperback of The Role of Microenvironment in ... cell culture techniques; surgical procedures; tissue procedures; tissue processing; histological methods; immuno-histochemical ... Series: Advances in Anatomy, Embryology and Cell Biology. Audience: General Format: Paperback Language: English Number Of Pages ... It also demonstrates the strong growth-promoting actitivies of implanted glial cells; these studies could have major practical ...
13 Electrospun Nano﫿berfor Three Dimensional Cell Culture 417. Yashpal Sharma, Ashutosh Tiwari and Hisatoshi Kobayashi ... 3.6.4 In Vivo MR Microimaging Technique of Rat Skin Exposed to CNT 127 ... 3.6.2 Static Model: 3-Dimensional Tissue Engineered Lung 124. 3.6.3 Dynamic Model: Integration of 3D Engineered Tissues into ... 14 Magnetic Nanoparticles in Tissue Regeneration 435. Anuj Tripathi, Jose Savio Melo and Stanislaus Francis DSouza ...
Purchase Biomedical Foams for Tissue Engineering Applications - 1st Edition. Print Book & E-Book. ISBN 9780857096968, ... 3.4 Pore structure versus in vitro cell culture. 3.5 Pore structure vs. in vivo new tissue regeneration ... 3.2 Micro-structure of biomedical foams and processing techniques. 3.3 Improving control of scaffold pore structure by combined ... whose structure can be engineered to meet the requirements of nutrient trafficking and cell and tissue invasion, and to tune ...
The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of ... The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three- ... dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) ... extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue. ...
Plant Cell and Tissue Culture: PPH-30306. *Regulation of Plant Development: MOB 30806 ... Advanced Cellular Imaging Techniques: CLB-30306. *Plant Biotechnology: GEN-20806. *Bioresources: PBR-31306 ...
... labeling technique for performing immunocytochemistry and in situ hybridization in virus infected cell cultures and tissues. / ... A double labeling technique for performing immunocytochemistry and in situ hybridization in virus infected cell cultures and ... A double labeling technique for performing immunocytochemistry and in situ hybridization in virus infected cell cultures and ... labeling technique for performing immunocytochemistry and in situ hybridization in virus infected cell cultures and tissues. ...
... this handy paperback describes modern tissue culture methods within a conceptual framework of cell biology, genetics, nutrition ... The books unique contribution is its rendering of technical cell culture information in a comprehensive, logical format. ... Introduction to cell and tissue culture : theory and technique / Jennie P. Mather and Penelope E. Roberts. August 26, 2014. Ann ... while exercises and techniques stimulate thought about the broader aspects of cell culture. Nearly 100 illustrations, including ...
Erratum: Development of a refined tenocyte differentiation culture technique for tendon tissue engineering (Cells Tissues ... Erratum: Development of a refined tenocyte differentiation culture technique for tendon tissue engineering (Cells Tissues ...
Humans , Connective Tissue Cells , Cell Culture Techniques/methods , Bioengineering/methods , Gingiva/cytology , Cell Biology ... Full text: Available Index: LILACS (Americas) Main subject: Connective Tissue Cells / Cell Culture Techniques / Bioengineering ... Collection and culture of human connective tissue cells from gingival explant technique for oral tissue bioengineering / ... Collection and culture of human connective tissue cells from gingival explant technique fo ...
Evaluation of smooth muscle cell phenotype is often done in place of functional testing in engineered tissues, assuming a ... Cell Culture Techniques. Collagen* / metabolism. Immunohistochemistry. Rats. Tissue Engineering*. Tunica Media / anatomy & ... Due to large differences in cell-compaction and cell distribution in the tissues, no correlation was found between α-actin ... Evaluation of smooth muscle cell phenotype is often done in place of functional testing in engineered tissues, assuming a ...
... tissue and organ culture : fundamental methods. [O L Gamborg; Gregory C Phillips;] -- A manual providing all relevant protocols ... for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis ... Techniques de culture dorganes--Manuel de laboratoire. a schema:Intangible ;. schema:name "Techniques de culture dorganes-- ... tissue_manuel_de_laboratoire> # Culture tissue--Manuel de laboratoire. a schema:Intangible ;. schema:name "Culture tissue-- ...
This webinar reviews some of the systems available,best practices when imaging 3D cell culture mode... ... There are many important considerations for how best to image a 3D cell culture model. Moreover, there are numerous high ... Apply tissue clearing techniques to fully characterize 3D cell culture models in a single workflow. •Utilize water immersion ... 3D Cell Cultures and Tissue Clearing. There are many important considerations for how best to image a 3D cell culture model. ...
Introduction to Cell and Tissue Culture from Dymocks online BookStore. Find latest reader reviews and much more at Dymocks ... It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The- ory and Techniques by Mather and ... ebook) Introduction to Cell and Tissue Culture Estimated Delivery:. Between Tuesday 18th December - Thursday 20th December when ... ebook) Introduction to Cell and Tissue Culture Penelope E. Roberts, Jennie P. Mather ...
... the cells are first swollen in a buffered hypo‐osmotic medium containing potassium chloride, magnesium acetate and ... Techniques in Cell Biology Contact Editor Submit a note to the editor about this article by filling in the form below. * ... Graham JM (1993) Isolation of membranes from tissue culture cells. In: Graham JM and Higgins JA (eds) Methods in Molecular ... Graham, John M(Mar 2004) Homogenization of Tissue Culture Cells in Suspension. In: eLS. John Wiley & Sons Ltd, Chichester. http ...
Screening techniques were developed to obtain disease (Fusarium wilt, black Sigatoka) and nematode resistant plants, while ... entitled Cellular biology and biotechnology including mutation techniques for creation of new useful banana genotypes . In 1996 ... Plant Cell Tissue Organ Culture 66 (2001) 189-197.. [33] SAS INSTITUTE INC. 1996. SAS/STAT Users Guide release 6 (12th ed.), ... In vitro culture, radiosensitivity and mutation induction. In vitro cultures of the cultivar Lakatan were provided by the IPB ...
  • 4)Musculoskeletal engineering: 1) Mechanism's for directing cell differentiation in 3D models of tissue constructs 2) Cell sourcing, using mechanical cues and monitoring through mechano-responsive genes in tissue engineered tendon, skin and muscle constructs 3) Identification of molecular and cellular markers which respond to defined mechanical cues. (
  • and growth, differentiation and organogenesis in plant tissue and organ cultures. (
  • Each technique can be used to investigate a wide spectrum of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to control of proliferation and differentiation. (
  • For over 30 years, Corning Matrigel matrix has been widely used in a variety of applications such as general cell culture, in vivo studies, hESC or organoid cultures and differentiation. (
  • The lectures focus on germline stem cells and their differentiation into functional gametes through the processes of spermatogenesis and folliculogenesis, maturation of gametes and acquisition of fertilization and developmental competence, sperm-egg interaction, egg activation and preimplantation embryo development. (
  • Efforts to induce vascular growth into tissue-engineered scaffolds have recently been dedicated to developing novel strategies to deliver specific biological factors that direct the recruitment of endothelial cell (EC) progenitors and their differentiation. (
  • Corning Matrigel Matrix is a reconstituted basement membrane preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors and is widely used for a variety of applications such as general cell culture, in vivo study, hESC or organoid culture and differentiation. (
  • Differentiation into this state is primarily completed before birth with the cells continuing to grow in size thereafter. (
  • Heilshorn's interests include biomaterials in regenerative medicine, engineered proteins with novel assembly properties, microfluidics and photolithography of proteins, and synthesis of materials to influence stem cell differentiation. (
  • 1 Basic Techniques for Mammalian Cell Tissue Culture UNIT 1.1 Tissue culture technology has found wide application in the field of cell biology. (
  • Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. (
  • Written for both novices and experts, this handy paperback describes modern tissue culture methods within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology. (
  • In this book, Mather and Roberts present the relevant method- ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for- mat. (
  • Journal of Cell Biology 97: 270-275. (
  • Journal of Cell Biology 104: 875-886. (
  • Section 1 laboratories teach methods key to studying hormone action and cell biology. (
  • He has over 25 years experience in cell biology with specific interests in cell culture technology, tissue engineering and stem cell research. (
  • Series: Introductory Cell and Molecular Biology Techniques. (
  • Keep informed of new developments and technical advances in light microscopy in the fields of molecular and cell biology by reading primary literature and microscopy and manufacturer websites. (
  • Describe and perform molecular biology and genetic engineering techniques with particular emphasis on restriction digests, cloning, Southern blotting, Western blotting, PCR, and DNA typing. (
  • Describe the applications of molecular biology and genetic engineering techniques in human health and welfare, medicine, agriculture and the environment. (
  • Cell- and tissue culture techniques , 3D Printing in biology,3D bird eggs. (
  • Our primary goal is to develop human biology-based in vitro platforms for disease modelling and toxicity testing-areas of interest include human stem cell-based disease modelling, cellular and tissue engineering, synthetic biology, in vitro & computational toxicology, and science policy. (
  • It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs. (
  • In addition, recent progress in stem cell biology has enabled iPS cells to be efficiently differentiated and purified into subtypes of cardiomyocytes, such as ventricular, atrial and nodal cells as well as smooth muscle cells and endothelial cells. (
  • New technologies such as genetic engineering, systems biology, protein engineering, and mammalian cell and plant cell systems are expanding rapidly, as is the demand for sustainable production of bioingredients, drugs, bioenergy and biomaterials. (
  • M. Phelan, "Basic techniques for mammalian cell tissue culture," Current Protocols in Cell Biology , pp. 15-35, 1998. (
  • Fibrosis consists mainly of cells called 'fibroblasts' and other extracellular matrix components secreted by fibroblasts. (
  • Considering this, the research team came up with a simple strategy: mix and culture pancreatic cancer cells and fibroblasts at various ratios. (
  • The team successfully showed that by varying the ratio of pancreatic cancer cells seeded against a fixed number of fibroblasts, they could create pancreatic cancer tissues in vitro with different amounts of fibrosis. (
  • 1) Investigate and test mechanisms for spatial orientation and effect of mechanical cues on cellular responses in 3D with applications in bioreactor technology 2) Investigate and identify cell sources, (stem cells, dermal fibroblasts, tendon fibroblasts and myoblasts) for mechanical and spatial cue responses. (
  • New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. (
  • Gingival fibroblasts present structural function, being able to modulate their metabolic capacity, which is reflected in the tissue morphology. (
  • The possibility of culturing fibroblasts in vitro , in monolayer or on three-dimensional scaffolds, for subsequent transplants in vivo opens important perspectives for the periodontal surgical clinic. (
  • Aging of Cultured Human Skin Fibroblasts. (
  • Fibroblasts and stromal cells have previously mainly been considered to provide tissue integrity, but it has become evident that they also play an active role in directing and controlling immune responses. (
  • It is characterized by a dense stroma with activated fibroblasts, in which tumor cells are embedded. (
  • Cancer-associated fibroblasts have been shown to promote tumor growth, but the interactions between T-cells and cancer-associated fibroblasts is poorly understood. (
  • Since iPS cells may be generated from somatic cells, such as skin fibroblasts or blood cells, they offer an ethical advantage over embryonic stem cells. (
  • The global cell and tissue culture supplies market is driven by factors such as increasing investments on R&D for cell-based research and tissue engineering, growing demand for artificial organs, rising need for serums and vaccines, and among others. (
  • There is an unmet need for artificial tissue to address current limitations with donor organs and problems with donor site morbidity. (
  • Direct 3D printing presents rapid, bespoke, acellular solutions for skull and bone repair or replacement, and can potentially address the need for elastic tissue, which is a major constituent of smooth muscle, cartilage, ligaments and connective tissue that support organs. (
  • A technique for maintenance or growth of animal organs in vitro. (
  • In conclusion, culture of explant of human gingival connective tissue is a viable method for obtaining gingival connective tissue cells suitable for laboratory tests in cell culture , aiming at obtaining constructs for gingival tissue engineering . (
  • Analysis of lung tissue RNA using Ingenuity Pathway Analysis (IPA) indicated trends in inflammatory response and connective tissue organization markers, with an emphasis on immune cell trafficking, phagocytosis, and adaptive immune responses. (
  • The fibres and muscles are surrounded by connective tissue layers called fasciae . (
  • Connective tissue is present in all muscles as fascia . (
  • enclosing each fascicle is a layer called the perimysium , and enclosing each muscle fiber is a layer of connective tissue called the endomysium . (
  • skin is substantially influenced by the organization of connective fibers and underlying subcutaneous tissue. (
  • In that cases mucogingival surgery , such as connective tissue grafts, is necessa. (
  • Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries. (
  • The next three chapters deal with placental hemodynamics, use of placental tissues and cells in pharmacological assays, and placental transfer of drugs used in pregnancy. (
  • Many different recipes for tissue culture media are available and each laboratory must determine which medium best suits their needs. (
  • To this end, researchers at Okayama University, Japan, including Professor Mitsunobu R. Kano and Assistant Professor Hiroyoshi Y. Tanaka, found a way to recreate pancreatic cancer tissue 'in vitro' (in the laboratory). (
  • In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. (
  • The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. (
  • Potential activation of mast cells by IL-17A was assessed by gene expression analysis of the Laboratory of Allergic Diseases 2 (LAD2) mast cell line. (
  • With this volume, it should be possible to establish and maintain a cell culture laboratory devot- ed to any of the many disciplines to which cell culture methodology is applicable. (
  • A non-radioactive silver staining technique for AFLP markers suitable for local laboratory conditions has been established. (
  • Scientists have long cultured them in the laboratory (in vitro) using different methods and under a variety of conditions. (
  • By making an objective assessment of these different laboratory techniques, we found that some may have more of an edge over others in better replicating a natural state," Huang said. (
  • Their laboratory is currently pursuing two research objectives using electrophysiological, cell/molecular, neurochemical, anatomical and computational techniques. (
  • Train laboratory staff on different experimental techniques used in the core facility. (
  • Good laboratory technique and superior problem-solving, organizational, and interpersonal skills are required. (
  • Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition , addresses the most current principles and methods in plant tissue culture research. (
  • New topic discussions and laboratory exercises in the Second Edition include "Micropropagation of Dieffenbachia," "Micropropagation and in vitro flowering of rose," "Propagation from nonmeristematic tissue-organogenesis," "Variation in culture" and "Tissue culture of ferns. (
  • In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition , is a veritable harvest of information for the continued study and research in plant tissue culture science. (
  • The appeal of this practical manual is that it contains very detailed information on how to establish a tissue culture laboratory, from provision of lists of equipment and chemicals and their suppliers, to procedures and protocols for preparing media and carrying out experiments…I would recommend this book highly for teachers and researchers in plant tissue culture as a valuable resource for practical plant tissue culture. (
  • Median mRNA values of all genes studied were higher in tumors than in paired normal tissues. (
  • In agreement with data on STS cell lines, significant up-regulation for uPA and PAI-1 genes compared to reference values was seen. (
  • Expression of Foreign Genes in Cultured Insect Cells Using a Recombinant Baculovirus Vector. (
  • IPA analysis of the 280 significantly differentially expressed genes in the whole blood was suggestive of increased hematopoiesis and cell development, activation of cancer/tumor development pathways, and atopy. (
  • To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. (
  • Traditional techniques inserted the genes randomly into the host's genome. (
  • It is virtually impossible to talk about the conservation and sustainable use of genes, species and ecosystems separate from human cultures. (
  • 3. The plant cell of claim 2 wherein the chloroplast insertion site locus is between the trnV and rps7 genes in the inverted repeat regions of the chloroplast genome. (
  • The presence of IL-17A-positive mast cells was assessed by immunohistochemistry in synovial tissue obtained before and after secukinumab treatment, as well as in skin and gut tissues from SpA-related conditions. (
  • 3) Testing tissue engineered constructs in in vivo models specially developed using measurable outcomes. (
  • La posibilidad de cultivar fibroblastos in vitro , en monocapa o en andamios tridimensionales, para trasplantes posteriores in vivo abre perspectivas importantes para la clínica de cirugía periodontal. (
  • Though it has been known that culture techniques can affect what kind of cells pluripotent stem cells eventually become, no "gold standard" has yet been established to help scientists determine how the artificial environment can more closely replicate that found in a natural state (in vivo). (
  • Primary cells can more closely mimic an in vivo -like state and generate more physiologically relevant data than immortalized cells. (
  • It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (
  • Transfection and Transformation of Human Thyroid Epithelial Cells. (
  • Our goal was achieved by isolation and transfection of adipose-derived stromal cells (ADSCs) with adenovirus encoding the cDNA of VEGF. (
  • Transfection and preparation of stably transfected cell lines. (
  • The purified DNA is ready for all applications, including sensitive automated sequencing and mammalian cell transfection. (
  • Endotoxins are toxic to mammalian cell cultures and can greatly affect transfection efficiencies. (
  • LipoTAXI transfection reagent 3,4 and calcium phosphate 5 reagent methods were used to transfect StrataPrep endotoxin-free purified DNA into Cos-7, DU 145, and JAR cell lines and resulted in higher transfection efficiencies, compared to anion exchange enndotoxin-free purified plasmid DNA ( Figure 5 ). (
  • I have the cells grown on coverslips 16 hours prior to transfection. (
  • I can see that the transfection has worked when I try to image these cells, but they're so unhealthy that I don't want to trust the data. (
  • This report describes a combined immunocytochemical and in situ hybridization procedure which allows visualization of cellular or viral antigens and viral RNA in the same cell. (
  • Cultures infected with visna or measles virus were fixed in periodate-lysine-paraformaldehyde-glutaraldehyde, stained by the avidin-bio-tin-peroxidase technique using antibodies to viral or cellular proteins and then incubated with radiolabeled specific DNA probes (in situ hybridization). (
  • The in situ hybridization signal (silver grains/cell) on peroxidase-stained cells was reduced relative to hybridization with unstained cells. (
  • In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears. (
  • Nonspecific 'sticking' of probes over peroxidase stained cells was prevented by incorporation of 0.1% Triton X-100 into the hybridization solution and the post-hybridization washes. (
  • We and others have previously shown that placental stromal cells have an immunoregulatory capacity and can suppress T cell responses. (
  • A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. (
  • 2) Research Design and Participation - aiding the Director and independently assisting users in the design of experiments involving light microscopy methods including confocal and live cell microscopy, lightsheet microscopy, total-internal-reflection (TIRF) microscopy, multi-photon microscopy, and super-resolution microscopy (PALM/STORM, SIM, and Airyscan). (
  • Incumbent will also assist with advanced tissue preparation methods (e.g. tissue clearing, expansion microscopy), image processing (e.g. deconvolution), and data analysis methods (e.g. (
  • Design, and carry out experiments involving collection and processing of tissues and tissue culture cells and their analysis by immunofluorescence, live-cell imaging, confocal, two-photon, lightsheet, TIRF and super-resolution microscopy. (
  • Perform advanced sample preparation for high resolution light microscopy techniques, such as tissue clearing and expansion microscopy. (
  • 9. A method as claimed in claim 8 using bright field microscopy at low magnification to locate the desired cells. (
  • 11. A method as claimed in claim 10 comprising revisiting the desired cells to carry out a rapid examination of fine features with a different mode of microscopy from the coarse scan and by scanning in two dimensions in the magnified image of the desired cells. (
  • Tools for studying cells including histology, different types of microscopy, tissue culture and specialised cell staining techniques. (
  • You will learn retinal anatomy and the techniques of ocular microdissection, cryosectioning, plastic sectioning and fluorescence microscopy. (
  • Draw cell suspension into a Pasteur pipet and rinse cell layer two or three times to dissociate cells and to dislodge any remaining adherent cells. (
  • The research was supported by the National Institutes of Health and UCLA Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research. (
  • Tissue Culture of Skeletal Muscle. (
  • To better understand why sensory neurons express voltage-gated Na + channel isoforms that are different from those expressed in other types of excitable cells, we compared the properties of the hNE sodium channel [a human homolog of PN1, which is selectively expressed in dorsal root ganglion (DRG) neurons] with that of the skeletal muscle Na + channel (hSkM1) [both expressed in human embryonic kidney (HEK293) cells]. (
  • Differential effects of Latrunculin-A on myofibrils in cultures of skeletal muscle cells: insights into mechanisms of myofibrillogenesis. (
  • Dynamics of Z-band based proteins in developing skeletal muscle cells. (
  • Assembly of the skeletal muscle cell. (
  • Inhibitors arrest myofibrillogenesis in skeletal muscle cells at early stages of assembly. (
  • Myofibrillogenesis in skeletal muscle cells in the presence of taxol. (
  • Myofibrillogenesis in skeletal muscle cells. (
  • A skeletal muscle refers to multiple bundles ( fascicles ) of cells joined together called muscle fibers. (
  • Graham JM (1993) Isolation of membranes from tissue culture cells. (
  • Content includes separation techniques of biological macromolecules such as nucleic acids and proteins, growing bacteria, isolation of DNA, DNA cloning, and PCR. (
  • Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue. (
  • The success of the isolation can be analyzed by cell viability test (Trypan Blue exclusion test) and by flow cytometric analysis to assess apoptosis. (
  • Support Protocols describe freezing of monolayer cells, thawing and recovery of cells, counting cells using a hemacytometer, and preparing cells for transport. (
  • The project successfully adapted established protocols for mutation induction and subsequent propagation of in vitro cultured shoot tips [15] in two Philippine banana cultivars. (
  • The use of primary cells as a model system is expanding and so are the protocols in which they are used. (
  • Personnel are required to optimise equipment set-up, media and growth techniques. (
  • An important factor influencing the growth of cells in culture is the choice of tissue culture medium. (
  • Secondary cultures are checked for growth, fed periodically, and may be subsequently subcultured to produce tertiary cultures, etc. (
  • The time between passaging cells depends on the growth rate and varies with the cell line. (
  • Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. (
  • The aim of this study was to detect alterations in the urokinase-type plasminogen system, involved in tumor growth and invasion, by comparing mRNA levels of its components with those of paired normal tissues, and relating them with patient clinical course. (
  • Professor Przyborski is developing new and innovative ways to manage the growth and function of cultured cells. (
  • Utilizing the upstream regulator (i.e. transcription factors, cytokines, growth factors) analysis, the lung had 285, blood cells 30, aorta 39, and heart 32 significantly altered mediators 4 h post-exposure. (
  • Complexity of cell culture techniques and moral concern over mismanagement of animals may hamper the market growth. (
  • The growth is attributed to rising need for automated cell culture process, increasing demand for drug discovery and increasing number of people suffer from chronic diseases. (
  • One of the fundamental principles underlying tissue engineering approaches is that newly formed tissue must maintain sufficient vascularization to support its growth. (
  • In this approach, the neotissue can be constructed by using a combination of specific cells, growth factors, and 3D porous scaffolds alone or in combination ( 3 ). (
  • To promote biomaterial vascularization, a number of strategies have been developed by using ( i ) growth factors that induce endothelial cell (EC) migration and proliferation ( 9 ⇓ - 11 ) and ( ii ) ECs or stem cells that can differentiate toward the endothelial lineage ( 12 , 13 ). (
  • But unlike immortalized cells, primary cells have complex nutritional needs and require optimized growth conditions. (
  • To test this hypothesis, AIR Lab students are examining the effects of harvested secretions from normal and CF airway cells on P. aerugionsa growth, flagellar motility, and biofilm formation in vitro. (
  • [2] These cells are normally quiescent but can be activated by exercise or pathology to provide additional myonuclei for muscle growth or repair. (
  • From quantitative tissue culture, the "gold standard" in microbiology and medical diagnosis, to the development and growth of new tissue culture cell lines, standard research methods are being optimized and adapted for new applications, and new techniques are being developed and tested. (
  • d) repeating steps (b) and (c) as necessary to produce extracellular matrix proteins in the three dimensional stromal culture. (
  • Expression of green or red fluorescent protein (GFP or DsRed) linked proteins in non-muscle and muscle Cells. (
  • 8. The plant cell of claim 7 wherein the soluble RUBISCO enzyme comprises at least about 30% of the soluble proteins of the cell. (
  • Gibco Serum-Free Media (SFM), available for a variety of cell lines, offers numerous advantages over serum-supplemented cell culture, and our Protein-Free Media (PFM) eliminate many of the concerns involved with the use of animal proteins. (
  • Our team of scientists has developed a number of new cell and tissue culture products , including our CD CHO (Chinese Hamster Ovary) Optimization Kit, designed to maximize proteins yields in your CHO system, and TrypLE Select, a non-animal, room temperature stable trypsin replacement. (
  • Culture of Human Brain Tumors on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells and Cultured Human Glioma Cells. (
  • Adult neural stem cells are thought of as having a lot of potential for therapeutic applications, but they are fragile and difficult to keep alive in cell culture media (Petri dishes), making them a challenge to study. (
  • In order to provide a more hospitable environment, researchers at Rensselaer Polytechnic Institute are working on printing 3-D tissues that will stay alive and supply the neural stem cells with nutrients and oxygen. (
  • 4) Mesenchymal stem cells with applications in Musculoskeletal Engineering. (
  • The labs in Section 2 focus on stem cell transplantation and meiotic staging of germ cells, follicular culture and in vitro techniques related to maturation of oocytes, sperm physiology, fertilization of various animal models, intracytoplasmic sperm injection, and other micromanipulation procedures. (
  • New research from UCLA establishes a standard to assess how conditions used to procure stem cells in the lab compare to those found in a human embryo, and could provide the foundation for scientists to more precisely replicate natural stem cell development in an artificial environment. (
  • The study, which was led by Guoping Fan, professor of human genetics at David Geffen School of Medicine at UCLA, was published in the October edition of the journal Cell Stem Cell . (
  • Pluripotent stem cells are cells that can transform into almost any cell in the human body. (
  • These previously published studies used different culture methods newly developed in vitro in the hopes of coaxing human stem cells into a type of pluripotency that is in a primitive or ground-zero state. (
  • We hope this work will help the research community to reach a consensus to quality-control human pluripotent stem cells," Fan said. (
  • Derivation and Maintenance of Embryonic Stem Cell Cultures. (
  • See other webinars in this series on our webinar page - Advanced Trends and Tools for Immune Cell Therapy and Advanced Trends and Tools for Stem Cell Therapy. (
  • Induced Pluripotent Stem Cell-Based Disease Modeling and Drug Discovery: Can We Recapitulate Cardiovascular Disease on a Culture Dish? (
  • Retinoic acid promotes neural and represses mesodermal gene expression in mouse embryonic stem cells in culture. (
  • Suppression of SHP‐2 and ERK signalling promotes self‐renewal of mouse embryonic stem cells. (
  • Human ES cell‐derived neural rosettes reveal a functionally distinct early neural stem cell stage. (
  • Current projects include tissue engineering for spinal cord and blood vessel regeneration, designing injectable materials for use in stem cell therapies, and the design of microfluidic devices to study the directed migration of cells (i.e., chemotaxis). (
  • For animals, the gene is typically inserted into embryonic stem cells, while in plants it can be inserted into any tissue that can be cultured into a fully developed plant. (
  • Plant cell, tissue and organ culture. (
  • A novel striated tropomyosin incorporated into organized myofibrils of cardiomyocytes in cell and organ culture. (
  • Cell culture is an important tool in medical, odontological and biological research laboratories , supporting cell therapies and tissue bioengineering strategies . (
  • Tissues were collected 4 h and 28 d post-exposure, RNA was isolated and microarray results were analyzed using Ingenuity Pathway Analysis for pertinent biological and molecular networks associated with effects. (
  • A method of introducing biological material into cells includes providing one or more target cells and establishing a spray of substantially dispersed particles including biological material. (
  • An apparatus for introducing biological material into one or more target cells includes a biological material source including at least biological material and a dispensing device operably connected to the biological material source to receive biological material therefrom. (
  • The chemical constituents of living cells and biological reactions. (
  • This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture. (
  • The loss of cultural diversity and traditional knowledge - of farm communities, languages, and indigenous cultures - is intricately linked to the loss of biological diversity. (
  • It is a form of striated muscle tissue , which is under the voluntary control of the somatic nervous system . (
  • Establishment of Mouse Epithelial Lung Cell Strains and Cell Lines. (
  • Human Thyroid Epithelial Cells. (
  • Covers methods for creating mammalian fibroblastic cell cultures and maintaining culture conditions for epithelial, neuronal, and hematopoietic cells among others. (
  • However, experimental models that are used to study pancreatic cancer have not yet been able to fully replicate the extent of fibrosis in human tissue. (
  • Using a three-dimensional (3D) cell culture technique, they devised a method to mix pancreatic cancer cells together with fibrotic components to generate tissues that resemble human pancreatic cancer. (
  • In pancreatic cancer, fibrotic tissue often occupies much more space than the cancer cells themselves. (
  • Thus, the scientists reasoned that it was important to establish relevant pancreatic cancer models that comprise not only cancer cells but also cells involved in fibrosis. (
  • There were other challenges as well: an analysis by the research team revealed that the area occupied by fibrosis in human pancreatic cancer tissues ranges from 40 to 80% among patients. (
  • Prof Kano says, 'There was considerable heterogeneity among patients, and for our 3D cell culture model to fully resemble human pancreatic cancer, we wanted to make sure that we could recreate tissue with any given amount of fibrosis. (
  • Utilizing recently published gene expression profiles of human embryos that hadn't yet implanted as the benchmark to analyze the data, Huang and his colleagues found that the newer culture methods appear to better resemble those found in the natural environment of developing embryos. (
  • Whole blood gene expression profiling is a promising approach to identification of novel biomarkers for tissues over traditional, invasive biopsy. (
  • Helium Plasma Skin Regeneration: Evaluation of Skin Tissue Effects in a Porcine Model and Comparison to Nitrogen Plasma Skin Regeneration. (
  • Helium plasma skin regeneration (PSR) is a novel skin rejuvenation technology with significant differences compared with nitrogen PSR technology but that may exert similar skin tissue effects. (
  • This report analyzes the worldwide markets for Cell and Tissue Culture Supplies in US$ Thousands by the following Product Segments: Media, Sera, and Reagents. (
  • Biodegradable microfluidic scaffolds for tissue engineering from amino alcohol-based poly(ester amid. (
  • The utility of this approach was evaluated by using 3D poly(lactide-co-glycolide) (PLAGA) sintered microsphere scaffolds for bone tissue engineering applications. (
  • The success of tissue engineering strategies, however, critically depends on the extent of blood vessel infiltration into the scaffolds ( 4 ). (
  • The primary function of vasculature is to facilitate the transport of nutrients and oxygen to cells invading from the host tissue and cells transplanted in the scaffolds. (
  • Over the past few years, 3D Cell Culture has gained momentum within the pharmaceutical industry due to the benefits that this model offers for in vitro applications patient-derived tissues, drug discovery, predictivity and validation, and safety and toxicity. (
  • Endotoxin, prevalent in plasmid preparations, is difficult to separate from plasmid DNA and results in toxicity to cultured mammalian cells. (
  • Sreedevi, S. 2016-06-29 00:00:00 Explantation and trypsinisation methods for tissue dissociation were attempted for the establishment of primary cell cultures from the caerulean damsel, Pomacentrus caeruleus. (
  • Histological examination of skin tissue in the porcine animal model after simultaneous and consecutive application of monopolar radiofrequency and targeted pressure energy. (
  • CAUTION: When working with human blood, cells, or infectious agents, appropriate biosafety practices must be followed. (
  • Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today. (
  • IL-17A did not induce a pro-inflammatory response in human LAD2 mast cells according to the canonical IL-17A signalling pathway. (
  • Real-time PCR was performed on human STS cell lines and tissues from highly malignant STS, including leiomyosarcomas and malignant fibrous histiocytomas, to evaluate the expression of urokinase-type plasminogen activator (uPA), uPA receptor (uPAR) and plasminogen activator inhibitor-1 (PAI-1). (
  • These enabling technologies have multiple applications and will be particularly relevant to bioengineering models of human tissues to advance basic research and drug discovery. (
  • When you have culture conditions that more consistently match a non-artificial environment, you have the potential for a much better reflection of what is going on in actual human development," said Kevin Huang, postdoctoral fellow in Fan's lab and a lead author of the study. (
  • Establishment, Maintenance, and Cloning of Human Primary Cell Strains. (
  • Human Long-Term Bone Marrow Culture. (
  • In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells. (
  • Cytogenetic Techniques for Human Leukemias. (
  • My research also extends into animal models and human tissue as this is important in translation. (
  • Dr. Bankers-Fulbright's research in the Airway Inflammation Research (AIR) Lab focuses on the interactions of human airway cells with pathogens and immune cells. (
  • Mechanical characterisation of human and porcine scalp tissue at dynamic strain rates. (
  • This clinical study has been initiated to assess the effect of laser exposure on human skin and determine the threshold for tissue damage. (
  • To investigate the mechanisms of retinal iron regulation, the lab uses conditional knockout mouse models, human retinal tissue, and retinal cell tissue culture. (
  • Tissue culture, simply put, is the method of keeping tissue alive and growing in vitro in a tissue culture media containing a rich mixture of essential amino acids, vitamins, and peptides, thus replicating conditions in which the tissue normally grows in an animal or human. (
  • Transmission and Scanning Electron Microscope Preparation of Whole Cultured Cells. (
  • The resultant cell suspension is then subdivided, or reseeded, into fresh cultures. (
  • the Alternate Protocol 1 describes passaging of suspension cultures. (
  • Alternate Protocol 2 describes freezing of suspension cells. (
  • If cultures are to be split 1/3 or 1/4 rather than 1/2, add sufficient medium such that 1 ml of cell suspension can be transferred into each fresh culture vessel. (
  • Graham, John M(Mar 2004) Homogenization of Tissue Culture Cells in Suspension. (
  • Scale-Up of Suspension and Anchorage-Dependent Animal Cells. (
  • She furthered developed her career in Belgium, in the field of antibody production in suspension cell lines. (
  • Gamborgs B5 medium is widely used for cell suspension cultures (no ammonium). (
  • In recent years, classification of soft-tissue sarcomas (STS) has improved with cytogenetic analyses, but their clinical behavior is still not easily predictable. (
  • methods for phenotypic analyses employing culture systems/techniques to construct physiologically-relevant tissues such as three dimensional organoids. (
  • Maintenance of cells in long-term culture requires strict adherence to aseptic technique to avoid contamination and potential loss of valuable cell lines (see UNIT 1.3). (
  • Besides providing nutrients for growing cells, medium is generally supplemented with antibiotics, fungicides, or both to inhibit contamination. (
  • Using proper technique is imperative for avoiding cross-contamination that can ruin the sample and affect the entire cell culture process. (
  • The Syracuse Biologics Manufacturing operation produces clinical, commercial and launch biologics drug substance at its 5000L scale manufacturing facility (mammalian cell process) in Syracuse, New York. (
  • Researchers globally are realizing the potential of 3D cell culture for various applications, including models for personalized medicine, complex and multi-cell type models, translation and clinical and industrial applications. (
  • The clinical symptoms of most of these neurodegenerative diseases are not due to cell loss or the pathology, but the loss of connections between nerve cells, so they can no longer 'talk' to each other,' said Associate Professor King. (
  • Despite the success with sophisticated tissue engineering endeavours, which employ cells as building blocks, they are limited to dedicated labs suitable for cell culture, with associated high costs and long tissue maturation times before available for clinical use. (
  • NOTE: All solutions and equipment coming into contact with living cells must be sterile, and aseptic technique should be used accordingly. (
  • 2 Sterile Pasteur pipets 37 C warming tray or incubator Tissue culture plasticware or glassware including pipets and 25-cm 2 flasks or 60-mm petri plates, sterile NOTE: All culture incubations should be performed in a humidified 37 C, 5% CO 2 incubator unless otherwise specified. (
  • DMEM) may require altered levels of CO 2 to maintain ph Remove all medium from primary culture with a sterile Pasteur pipet. (
  • They also mimicked vascular channels that distribute oxygen and nutrients through the tissues. (
  • A major cost component of cell culture is fetal bovine serum (FBS), an often essential and very expensive. (
  • Cell cultures are utilized in cytogenetic, biochemical, and molecular laboratories for diagnostic as well as research studies. (
  • The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. (
  • provides assistance in acquiring those skills that are critical for plant tissue culture and plant biotechnology research. (
  • Cell Culture in an important tool for research and development within the life science industry. (
  • Sometimes we need to take a step backwards to do some basic research to see how nerve cells work and interact with each other. (
  • The scope of the report encompasses the major types of 3D cell culture that are being used by industry, academic researchers, government labs, and independent research groups. (
  • Although the limiting reasons for this matter may be attributed to "immature" properties of iPS cell-derived cardiomyocytes compared to the native ones, much remain to be solved in this area of research that is quickly gaining traction from researchers. (
  • This Research Topic welcomes papers tackling iPS cell-based disease modeling and/or drug discovery, and that address the above questions related to the cardiovascular field. (
  • This Research Topic hopes to foster discussion and potential strategies for iPS cell-based research in the cardiovascular fields. (
  • Tissue engineering of skin is a field with high research activities and major importance for wound healing, especially following burn injuries. (
  • Recent research in the lab indicates that inflammation promotes cellular iron overload in a vicious cycle leading to cell death. (
  • Gives in-depth information and the latest research on the vital concepts and techniques of in vitro plant breeding. (
  • Gibco® cell and tissue culture products, the broadest selection in the industry, are optimized to support innovative research and leading-edge applications. (
  • Our outstanding and innovative tissue culture products, services and resources keep your research on the cutting edge, maximizing yields while maintaining the superior quality and reliability for which we've become known in the industry. (
  • With these findings, Fan's team hopes to encourage further investigation into other cell characteristics and molecular markers that determine the effectiveness of culture conditions on the proliferation and self-renewal of PSCs. (
  • Finally, the isolated cells can be used for further investigations like comparative studies of mRNA expression, cell-proliferation assay or protein analysis. (
  • Compounds and compositions are provided for treatment, prevention, or amelioration of a variety of medical disorders associated with viral infections, cell proliferation and bone metabolism. (
  • The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. (
  • Explants derived from periodontal surgical discards were used, grown in 25 cm2 bottles to obtain a primary cell culture . (
  • Corning Life Sciences knows cells - you might even say it's in our DNA with over 100 years of experience in the field. (
  • In this pre-recorded webinar, Corning scientists put their expertise in the areas of 3D cell culture and bioprocess to work as they answered some of the commonly asked questions they receive. (
  • Building on a long and expert history in cell culture, Corning offers viral vector manufacturing platforms that are easily integrated into modular production set-ups, such as Corning® HYPERStack® and CellSTACK® vessels, which are enabling viral vectors to be produced at large scales. (
  • Trypsin 0.05%/0.53 mM EDTA for COS‐7 cells (Corning, cat. (
  • The goal of this project is to utilize computer-aided design and injection molding technologies to tissue engineer precisely-shaped cartilage in the shape of butterfly tympanic membrane patches out of chondrocyte-seeded calcium alginate gels. (
  • We will fabricate cartilage-seeded tympanic membrane patches using cell culture techniques. (
  • However, the number of vegetative propagation cycles for the shoot-tip technique used may not be sufficient to eliminate chimeras completely in the mutated populations. (
  • Induced mutation (either by chemicals or irradiation), coupled with in vitro propagation technique such as shoot-tip culture, has been established as a tool to generate variation in a number of vegetatively propagated crops [2-4]. (
  • Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. (
  • Many of these channels have specific developmental, tissue, or cellular distributions. (
  • Based on these advances, disease-specific iPS cells can be generated from patients with intractable cardiovascular diseases, such as inherited cardiomyopathies, to elucidate the molecular and cellular mechanisms underlying disease onset and/or the pathophysiological process. (
  • Studies on colonic cells in the lamina propria (LP) of mice are important for understanding the cellular and immune responses in the gut, especially in inflammatory bowel diseases (such as morbus crohn and colitis ulcerosa). (
  • The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue. (
  • Attention is given to the diversity of culture media and extracellular matrices needed to maintain the differential functions of the cultured cells. (
  • She is the author of more than 125 papers and twenty-five book chapters on biotechnology, biomechanics, orthopedics, and tissue engineering. (
  • Despite the occasional appearance of thought- ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. (
  • This methodology for building 3D structure can facilitate material anisotropy, which is an attractive feature when aiming for hetergenous tissue biomimicry. (
  • This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. (
  • These in vitro techniques include utilizing cell or tissue cultures, isolated cells. (
  • These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. (
  • In addition to the fundamentals, attention is also given to mod- ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. (