Tandem mass spectrometry. Medical search

A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.

An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.

A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.

Chromatographic techniques in which the mobile phase is a liquid.

A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.

Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.

The systematic study of the complete complement of proteins (PROTEOME) of organisms.

A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.

The protein complement of an organism coded for by its genome.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.

Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.

A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.

The identification of selected parameters in newborn infants by various tests, examinations, or other procedures. Screening may be performed by clinical or laboratory measures. A screening test is designed to sort out healthy neonates (INFANT, NEWBORN) from those not well, but the screening test is not intended as a diagnostic device, rather instead as epidemiologic.

A mass-spectrometric technique that is used for microscopic chemical analysis. A beam of primary ions with an energy of 5-20 kiloelectronvolts (keV) bombards a small spot on the surface of the sample under ultra-high vacuum conditions. Positive and negative secondary ions sputtered from the surface are analyzed in a mass spectrometer in regards to their mass-to-charge ratio. Digital imaging can be generated from the secondary ion beams and their intensity can be measured. Ionic images can be correlated with images from light or other microscopy providing useful tools in the study of molecular and drug actions.

The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.

The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.

A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.

Copies of DNA sequences which lie adjacent to each other in the same orientation (direct tandem repeats) or in the opposite direction to each other (INVERTED TANDEM REPEATS).

Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.

Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.

Errors in metabolic processes resulting from inborn genetic mutations that are inherited or acquired in utero.

Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.

Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.

Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)

An atom or group of atoms that have a positive or negative electric charge due to a gain (negative charge) or loss (positive charge) of one or more electrons. Atoms with a positive charge are known as CATIONS; those with a negative charge are ANIONS.

A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.

Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.

Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.

Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)

Mixtures of many components in inexact proportions, usually natural, such as PLANT EXTRACTS; VENOMS; and MANURE. These are distinguished from DRUG COMBINATIONS which have only a few components in definite proportions.

Methods for assessing flow through a system by injection of a known quantity of an indicator, such as a dye, radionuclide, or chilled liquid, into the system and monitoring its concentration over time at a specific point in the system. (From Dorland, 28th ed)

Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.

Deuterium. The stable isotope of hydrogen. It has one neutron and one proton in the nucleus.

A research technique to measure solvent exposed regions of molecules that is used to provide insight about PROTEIN CONFORMATION.

Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.

A chromatography technique in which the stationary phase is composed of a non-polar substance with a polar mobile phase, in contrast to normal-phase chromatography in which the stationary phase is a polar substance with a non-polar mobile phase.

The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)

Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.

Techniques for using whole blood samples collected on filter paper for a variety of clinical laboratory tests.

Atomic species differing in mass number but having the same atomic number. (Grant & Hackh's Chemical Dictionary, 5th ed)

The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.

A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.

Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992)

The pressure at any point in an atmosphere due solely to the weight of the atmospheric gases above the point concerned.

The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.

Compounds in which a methyl group is attached to the cyano moiety.

Stable oxygen atoms that have the same atomic number as the element oxygen, but differ in atomic weight. O-17 and 18 are stable oxygen isotopes.

Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).

Glycosides of GLUCURONIC ACID formed by the reaction of URIDINE DIPHOSPHATE GLUCURONIC ACID with certain endogenous and exogenous substances. Their formation is important for the detoxification of drugs, steroid excretion and BILIRUBIN metabolism to a more water-soluble compound that can be eliminated in the URINE and BILE.

Sequential operating programs and data which instruct the functioning of a digital computer.

The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc.

The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.

A plant genus of the family RANUNCULACEAE. Members contain a number of diterpenoid alkaloids including: aconitans, hypaconitine, ACONITINE, jesaconitine, ignavine, napelline, and mesaconitine. The common name of Wolfbane is similar to the common name for ARNICA.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.

The vapor state of matter; nonelastic fluids in which the molecules are in free movement and their mean positions far apart. Gases tend to expand indefinitely, to diffuse and mix readily with other gases, to have definite relations of volume, temperature, and pressure, and to condense or liquefy at low temperatures or under sufficient pressure. (Grant & Hackh's Chemical Dictionary, 5th ed)

Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.

A constituent of STRIATED MUSCLE and LIVER. It is an amino acid derivative and an essential cofactor for fatty acid metabolism.

Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.

Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.

A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).

The chemical and physical integrity of a pharmaceutical product.

The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.

Derivatives of phosphatidic acid in which the hydrophobic regions are composed of two fatty acids and a polar alcohol is joined to the C-3 position of glycerol through a phosphodiester bond. They are named according to their polar head groups, such as phosphatidylcholine and phosphatidylethanolamine.

A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)

The characteristic 3-dimensional shape of a carbohydrate.

Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.

Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

The products of chemical reactions that result in the addition of extraneous chemical groups to DNA.

Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.

Drugs and their metabolites which are found in the edible tissues and milk of animals after their medication with specific drugs. This term can also apply to drugs found in adipose tissue of humans after drug treatment.

A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.

The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.

Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)

A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.

An essential amino acid. It is often added to animal feed.

The sum of the weight of all the atoms in a molecule.

Drugs used by veterinarians in the treatment of animal diseases. The veterinarian's pharmacological armamentarium is the counterpart of drugs treating human diseases, with dosage and administration adjusted to the size, weight, disease, and idiosyncrasies of the species. In the United States most drugs are subject to federal regulations with special reference to the safety of drugs and residues in edible animal products.

Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.

Proteins found in any species of bacterium.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Devices for accelerating charged particles in a spiral path by a constant-frequency alternating electric field. This electric field is synchronized with the movement of the particles in a constant magnetic field.

The process of cleaving a chemical compound by the addition of a molecule of water.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.

The dynamic collection of metabolites which represent a cell's or organism's net metabolic response to current conditions.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

Proteins prepared by recombinant DNA technology.

Organic compounds containing a carbonyl group in the form -CHO.

A territory of Australia consisting of Canberra, the national capital and surrounding land. It lies geographically within NEW SOUTH WALES and was established by law in 1988.

Targeted comparative proteomics by liquid chromatography-tandem Fourier ion cyclotron resonance mass spectrometry. (1/5193)

In proteomics, effective methods are needed for identifying the relatively limited subset of proteins displaying significant changes in abundance between two samples. One way to accomplish this task is to target for identification by MS/MS only the "interesting" proteins based on the abundance ratio of isotopically labeled pairs of peptides. We have developed the software and hardware tools for online LC-FTICR MS/MS studies in which a set of initially unidentified peptides from a proteome analysis can be selected for identification based on their distinctive changes in abundance following a "perturbation". We report here the validation of this method using a mixture of standard proteins combined in different ratios after isotopic labeling. We also demonstrate the application of this method to the identification of Shewanella oneidensis peptides/proteins exhibiting differential abundance in suboxic versus aerobic cell cultures. (+info)

The identification of 3,4-MDMA from its mass equivalent isomers and isobaric substances using fast LC-ESI-MS-MS. (2/5193)

3,4-methylenedioxymethamphetamine (3,4-MDMA, "Ecstacy") and its 17 isomers and isobaric substances are studied using liquid chromatography (LC)-positive electrospray ionization-mass spectrometry (MS). 3,4-MDMA is a controlled substance, whereas in many countries the other studied isobaric compounds are not. A method for confirmation of the presence of 3,4-MDMA in drug seizures is developed and validated. Using single MS, the compounds produce an intense protonated molecule and some characteristic fragments; but tandem MS (MS-MS) is applied to enhance specificity. The MS-MS fragmentation is studied in order to distinguish 3,4-MDMA from the other 17 related compounds. However, the MS-MS spectra of 3,4-MDMA and six related compounds are very similar. Therefore, the LC-MS-MS method is developed for the unambiguous identification of 3,4-MDMA. The use of a monolithic column allows for 5-min gradient runs. This qualitative method is tested with 49 Ecstacy samples seized by the police. All results are congruent with the ones obtained with other methods. (+info)

Parallel ion parking: improving conversion of parents to first-generation products in electron transfer dissociation. (3/5193)

Electron-transfer dissociation (ETD) in a tandem mass spectrometer is an analytically useful ion/ion reaction technique for deriving polypeptide sequence information, but its utility can be limited by sequential reactions of the products. Sequential reactions lead to neutralization of some products, as well as to signals from products derived from multiple cleavages that can be difficult to interpret. A method of inhibiting sequential ETD fragmentation in a quadrupole ion trap is demonstrated here for the reaction of a triply protonated peptide with nitrobenzene anions. A tailored waveform (in this case, a filtered noise field) is applied during the ion/ion reaction time to accelerate simultaneously first-generation product ions and thereby inhibit their further reaction. This results in a approximately 50% gain in the relative yield of first-generation products and allows for the conversion of more than 90% of the original parent ions into first-generation products. Gains are expected to be even larger when higher charge-state cations are used, as the rates of sequential reaction become closer to the initial reaction rate. (+info)

Some structural properties of plant serine:glyoxylate aminotransferase? (4/5193)

The structural properties of photorespiratory serine:glyoxylate aminotransferases (SGAT, EC 2.6.1.45) from maize (Zea mays L.) and wheat (Triticum aestivum L.) leaves were examined. By means of molecular sieving on Zorbax SE-250 column and filtration through centrifugal filters it was shown that dimers of wheat enzyme (molecular mass of about 90 kDa) dissociate into component monomers (molecular mass of about 45 kDa) upon decrease in pH value (from 9.1 or 7.0 to 6.5). At pH 9.1 a 50-fold decrease of ionic strength elicited a similar effect. Under the same conditions homodimers of the maize enzyme (molecular mass similar to that of the wheat enzyme) remained stable. Immunoblot analysis with polyclonal antiserum against wheat seedling SGAT on leaf homogenates or highly purified preparations of both enzymes showed that the immunogenic portions of the wheat enzyme are divergent from those of the maize enzyme. The sequence of 136 amino acids of the maize enzyme and 78 amino acids of the wheat enzyme was established by tandem mass spectrometry with time of flight analyzer. The two enzymes likely share similarity in tertiary and quaternary structures as well as high level of hydrophobicity on their molecular surfaces. They likely differ in the mechanism of transport from the site of biosynthesis to peroxisomes as well as in some aspects of secondary structure. (+info)

Two-dimensional gas-phase separations coupled to mass spectrometry for analysis of complex mixtures. (5/5193)

Ion mobility spectrometry (IMS) has been explored for decades, and its versatility in separation and identification of gas-phase ions is well established. Recently, field asymmetric waveform IMS (FAIMS) has been gaining acceptance in similar applications. Coupled to mass spectrometry (MS), both IMS and FAIMS have shown the potential for broad utility in proteomics and other biological analyses. A major attraction of these separations is extremely high speed, exceeding that of condensed-phase alternatives by orders of magnitude. However, modest separation peak capacities have limited the utility of FAIMS and IMS for analyses of complex mixtures. We report 2-D gas-phase separations that join FAIMS to IMS, in conjunction with high-resolution and accuracy time-of-flight (TOF) MS. Implementation of FAIMS/IMS and IMS/MS interfaces using electrodynamic ion funnels greatly improves sensitivity. Evaluation of FAIMS/IMS/TOF performance for a protein mixture tryptic digest reveals high orthogonality between FAIMS and IMS dimensions and, hence, the benefit of FAIMS filtering prior to IMS/MS. The effective peak capacities in analyses of tryptic peptides are approximately 500 for FAIMS/IMS separations and approximately 10(6) for 3-D FAIMS/IMS/MS, providing a potential platform for ultrahigh-throughput analyses of complex mixtures. (+info)

Newborn screening of inherited metabolic diseases by tandem mass spectrometry. (6/5193)

Application of TMS technology in newborn screening has resulted in major expansion of disorder panel for metabolic diseases in recent years. This automated, multiplex testing methodology detects multiple analytes from single analysis of one blood spot, which leads to detection of 30-35 disorders of amino acids, organic acids, and fatty acids metabolism. The early identification of persons affected with inborn errors of metabolism has led to unexpected discoveries related to the natural history of the disorder or options for therapy. This article summarized (1) the basic principles of this technology and methodology. (2) Current status of application of this methodology in the United States, European countries and in China. (3) The positive impacts on the public health and advances in medical genetics. Finally (4) Challenges, issues and possible solutions. The purpose of this article aimed at introducing new technology and exploring the possibilities of implementing into developing countries where medical genetics is not developed and foreseeing the possible problems and obstacles. (+info)

Peptide-phospholipid cross-linking reactions: identification of leucine enkephalin-alka(e)nal-glycerophosphatidylcholine adducts by tandem mass spectrometry. (7/5193)

The covalent interactions between peptides and lipid oxidation products, with formation of Schiff and Michael adducts, are known to occur during free radical oxidative damage. In this study, leucine-enkephalin-glycerophosphatidylcholine alka(e)nal adducts were analyzed by electrospray tandem mass spectrometry (MS/MS). Upon collision-induced dissociation of the Leucine enkephalin-2-(9-oxo-nonanoyl)-1-palmitoyl-3-glycerophosphatidylcholine, an alkanal Schiff adduct observed at m/z 1187.7, the main product ions were attributed to the phosphocholine polar head and loss of the peptide. Also, product ions resulting from characteristic losses of phosphatidylcholines and cleavages of the peptide chain (mainly b-type) were observed. Additional product ions formed by combined peptide and phosphatidylcholine fragmentations were identified. The fragmentation pattern of the leucine enkephalin-alkanal Schiff adduct and the leucine enkephalin-alkenal phosphatidylcholine Schiff and Michael adducts were similar, although the loss of the peptide for the Michael adduct should occur through a distinct mechanism. These fragmentation pathways differ greatly from those described for peptide-lipid Schiff and Michael adducts, in which only peptide chain cleavages are reported, probably due to charge retention in the glycerophosphatidylcholine polar head in peptide-glycerophosphatidylcholine adducts. (+info)

Cytoskeletal components of an invasion machine--the apical complex of Toxoplasma gondii. (8/5193)

The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility are greatly impeded by lack of knowledge of its molecular composition. We have partially purified the conoid/apical complex, identified approximately 200 proteins that represent 70% of its cytoskeletal protein components, characterized seven novel proteins, and determined the sequence of recruitment of five of these proteins into the cytoskeleton during cell division. Our results provide new markers for the different subcompartments within the apical complex, and revealed previously unknown cellular compartments, which facilitate our understanding of how the invasion machinery is built. Surprisingly, the extreme apical and extreme basal structures of this highly polarized cell originate in the same location and at the same time very early during parasite replication. (+info)

Tandem mass spectrometry can be used to sequence proanthocyanidins. Oligomeric proanthocyanidins (OPC) strictly refer to dimer ... Li, Hui-Jing; Deinzer, Max L (2007). "Tandem mass spectrometry for sequencing proanthocyanidins". Analytical Chemistry. 79 (4 ... Monomers of proanthocyanidins can be characterized by analysis with HPLC and mass spectrometry. Condensed tannins can undergo ... using MALDI-TOF/TOF mass spectrometry". Analytical and Bioanalytical Chemistry. 402 (3): 1327-1336. doi:10.1007/s00216-011-5557 ...

https://en.wikipedia.org/wiki/Proanthocyanidin

... oil by electrospray and tandem mass spectrometry". Journal of the Science of Food and Agriculture. 86 (3): 445-452. doi:10.1002 ...

https://en.wikipedia.org/wiki/Pequi_oil

Tandem mass spectrometry: non-invasive, rapid method; a significant peak at C16 is indicative of generalized CPT II deficiency ... 2002). Tandem Mass Spectrometric Assay for the Determination of Carnitine Palmitoyltransferase II Activity in Muscle Tissue. ...

https://en.wikipedia.org/wiki/Carnitine_palmitoyltransferase_II_deficiency

Sleno L, Volmer DA (2004). "Ion activation methods for tandem mass spectrometry". Journal of mass spectrometry : JMS. 39 (10): ... It is the science that studies ions and molecules in the gas phase, most often enabled by some form of mass spectrometry. By ... Adiabatic ionization Mass-analyzed ion kinetic energy spectrometry Plasma (physics) Michael T. Bowers R. Graham Cooks Helmut ... Chemical Ionization Mass Spectrometry. I. General Introduction. IUPAC, Compendium of Chemical Terminology, 2nd ed. (the "Gold ...

https://en.wikipedia.org/wiki/Gas-phase_ion_chemistry

Identification of IQGAP1 by nanoelectrospray tandem mass spectrometry". The Journal of Biological Chemistry. 272 (24): 15419-25 ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. doi: ... Zhao C, Ma H, Bossy-Wetzel E, Lipton SA, Zhang Z, Feng GS (September 2003). "GC-GAP, a Rho family GTPase-activating protein ...

https://en.wikipedia.org/wiki/CDC42

by liquid chromatography/electrospray ionisation tandem mass spectrometry". Phytochemical Analysis. 19 (4): 335-41. doi:10.1002 ... When they are viewed in mass, such as with a spore print, the spores appear white. Observing with a light microscope reveals ...

https://en.wikipedia.org/wiki/Hygrophorus_eburneus

Kinter, Michael; Sherman, Nicholas E. (2000). Protein sequencing and identification using tandem mass spectrometry. New York, ... "Lung Cancer Serum Biomarker Discovery Using Glycoprotein Capture and Liquid Chromatography Mass Spectrometry". Journal of ...

https://en.wikipedia.org/wiki/Leroy_Hood

Identification of IQGAP1 by nanoelectrospray tandem mass spectrometry". J. Biol. Chem. 272 (24): 15419-25. doi:10.1074/jbc. ...

https://en.wikipedia.org/wiki/IQGAP1

Miles RR, Crockett DK, Lim MS, Elenitoba-Johnson KS (2006). "Analysis of BCL6-interacting proteins by tandem mass spectrometry ...

https://en.wikipedia.org/wiki/ZMYND8

Tandem mass spectrometry is usually employed for added specificity. Standard curves and internal standards are used for ... Covey TR, Lee ED, Henion JD (October 1986). "High-speed liquid chromatography/tandem mass spectrometry for the determination of ... Pharmacokinetics is often studied using mass spectrometry because of the complex nature of the matrix (often plasma or urine) ... There is currently considerable interest in the use of very high sensitivity mass spectrometry for microdosing studies, which ...

https://en.wikipedia.org/wiki/Pharmacokinetics

The usual method of analysis is tandem mass spectrometry. Jones, P. M.; Bennett, M. J. (2010). "Urine Organic Acid Analysis for ... Inherited Metabolic Disease Using Gas Chromatography-Mass Spectrometry". Clinical Applications of Mass Spectrometry. Methods in ...

https://en.wikipedia.org/wiki/Urine_organic_acids

Tandem mass spectrometry is then used to identify the peptides. Targeted proteomics using SRM and data-independent acquisition ... Bottom-up proteomics Mass spectrometry software Protein mass spectrometry Shotgun lipidomics Top-down proteomics Alves, P; ... As the peptides elute from the column, they are ionized and separated by m/z in the first stage of tandem mass spectrometry. ... The charged fragments are separated in the second stage of tandem mass spectrometry. The "fingerprint" of each peptide's ...

https://en.wikipedia.org/wiki/Shotgun_proteomics

"Using Tandem Mass Spectrometry for Metabolic Disease Screening Among Newborns". www.cdc.gov. Centers for Disease Control (CDC ... Most newborn screening uses tandem mass spectroscopy to detect biochemical abnormalities that suggest specific disorders. DNA- ... Clinical geneticists often work in tandem with a genetic counselor and play an important role in providing genetic testing, ... MA; McGuire, AL; Milko, LV; Mooney, SD; Parad, RB; Pereira, S; Petrikin, J; Powell, BC; Powell, CM; Puck, JM; Rehm, HL; Risch, ...

https://en.wikipedia.org/wiki/Elective_genetic_and_genomic_testing

Beisken, Stephan (2015). Informatics for Tandem Mass Spectrometry-based Metabolomics (PDF) (PhD thesis). University of ... "LipidHome: A Database of Theoretical Lipids Optimized for High Throughput Mass Spectrometry Lipidomics". PLoS ONE. 8 (5): ...

https://en.wikipedia.org/wiki/Christoph_Steinbeck

Biemann K, Scoble HA (1987). "Characterization by tandem mass spectrometry of structural modifications in proteins". Science. ... Annexin A1 protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Glucocorticoids ...

https://en.wikipedia.org/wiki/Annexin_A1

by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry". Journal of Pharmaceutical and ...

https://en.wikipedia.org/wiki/Sophora_flavescens

Using Liquid Chromatography Tandem Atmospheric Pressure Chemical Ionization Mass Spectrometry". Phytochemical Analysis. 18 (5 ... "Liquid Chromatography-Electrospray Ionization Mass Spectrometry Study of the Flavonoids of the Roots of Astragalus mongholicus ... Xu, Q.; Ma, X.; Liang, X. (2007). "Determination of Astragalosides in the Roots of Astragalus spp. ...

https://en.wikipedia.org/wiki/Astragalus_propinquus

"Search for new alkaloids in Pachycereus weberi by tandem mass spectrometry". Analytical Chemistry. 57 (1): 109-114. doi:10.1021 ...

https://en.wikipedia.org/wiki/Anhalidine

"Detection and Quantitation of Circulating Human Irisin by Tandem Mass Spectrometry". Cell Metabolism. 22 (4): 734-40. doi: ... Via mass spectrometry, irisin has been found to circulate in humans in levels similar to other key hormones, such as insulin. ... Pedersen BK, Febbraio MA (October 2008). "Muscle as an endocrine organ: focus on muscle-derived interleukin-6". Physiological ... Wrann CD, White JP, Salogiannnis J, Laznik-Bogoslavski D, Wu J, Ma D, Lin JD, Greenberg ME, Spiegelman BM (November 2013). " ...

https://en.wikipedia.org/wiki/FNDC5

"Detection of neonatal argininosuccinate lyase deficiency by serum tandem mass spectrometry". J Inherit Metab Dis. 24 (3): 370-8 ...

https://en.wikipedia.org/wiki/Argininosuccinic_aciduria

The development of tandem mass spectrometry screening in the early 1990s led to a large expansion of potentially detectable ... Tandem mass spectrometry has greatly expanded the potential number of diseases that can be detected, even without satisfying ... ISBN 0-932727-91-3. Chace DH; Kalas TA; Naylor EW (2003). "Use of tandem mass spectrometry for multianalyte screening of dried ... With the advent of tandem mass spectrometry as a screening tool, several fatty acid oxidation disorders were targeted for ...

https://en.wikipedia.org/wiki/Newborn_screening

Mass spectrometry Tandem mass spectrometry Sector instrument Olah, George A. (2008). "Superelectrophiles and their chemistry". ... Holman, R. (1986). "Mass spectrometry for investigations of gas-phase radical cation chemistry the two step cycloaddition of ... Mass Spectrom. 17 (7): 1014-22. doi:10.1016/j.jasms.2006.03.008. PMID 16713292. Ibrahim Y, Mabrouki R, Meot-Ner M, El-Shall MS ...

https://en.wikipedia.org/wiki/Distonic_ion

... the use of Gas Chromatography coupled with tandem Mass Spectrometry (GC-MS/MS) and Isotope-ratio mass spectrometry (IRMS) is ... Identification of various banned small peptides in human urine using liquid chromatography tandem mass spectrometry. The ... Detection of Stanozolol conjugated metabolites by liquid chromatography tandem-mass spectrometry. Prednisone excretion study ... Rapid determination of urinary phthalates using liquid chromatography tandem mass spectrometry. ...

https://en.wikipedia.org/wiki/National_Dope_Testing_Laboratory

"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry". Nature Methods. 2 ...

https://en.wikipedia.org/wiki/TRIO_(gene)

"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry". Nature Methods. 2 ... Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale ...

https://en.wikipedia.org/wiki/BAG3

"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry". Nature Methods. 2 ... Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (February ...

https://en.wikipedia.org/wiki/DDX3X

indigo by column liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry". Journal of ...

https://en.wikipedia.org/wiki/Omphalotus_olivascens

"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry". Nat. Methods. 2 (8 ... Zhang Y, Wolf-Yadlin A, Ross PL, Pappin DJ, Rush J, Lauffenburger DA, White FM (2005). "Time-resolved mass spectrometry of ... "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry". Proc. Natl. Acad. Sci. U.S.A. 100 (2): ...

https://en.wikipedia.org/wiki/Plakophilin-4

... by tandem mass spectrometry". Biochem. Biophys. Res. Commun. 217 (1): 257-63. doi:10.1006/bbrc.1995.2772. PMID 8526920. Celis ... Dias Neto E, Correa RG, Verjovski-Almeida S, Briones MR, Nagai MA, da Silva W, Zago MA, Bordin S, Costa FF, Goldman GH, ... Gemmill RM, Bemis LT, Lee JP, Sozen MA, Baron A, Zeng C, Erickson PF, Hooper JE, Drabkin HA (2002). "The TRC8 hereditary kidney ...

https://en.wikipedia.org/wiki/S100A7

"Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry". Nat. Methods. 2 (8 ... tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/ ... Marra MA (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. ...

https://en.wikipedia.org/wiki/Drebrin-like

... tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry ... Ostendorff HP، Peirano RI، Peters MA، Schlüter A، Bossenz M، Scheffner M، Bach I (2002). "Ubiquitination-dependent cofactor ... "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.". Nat ...

https://ar.wikipedia.org/wiki/PDLIM1

Typically the presence of an amine functional group is deduced by a combination of techniques, including mass spectrometry as ... Weiberth, Franz J.; Hall, Stan S. (1986). "Tandem alkylation-reduction of nitriles. Synthesis of branched primary amines". ...

https://en.m.wikipedia.org/wiki/Amines

... lateriflora by liquid chromatography with ultraviolet photodiode array and electrospray ionization tandem mass spectrometry". ... Liu X, Hong SI, Park SJ, Dela Peña JB, Che H, Yoon SY, Kim DH, Kim JM, Cai M, Risbrough V, Geyer MA, Shin CY, Cheong JH, Park H ...

https://en.wikipedia.org/wiki/Scutellaria

Williamson BL, Johnson KL, Tomlinson AJ, Gleich GJ, Naylor S (October 1998). "On-line HPLC-tandem mass spectrometry structural ... These were characterized using accurate mass LC-MS, LC-MS/MS and multistage mass spectrometry (MSn).[16] The last of the six ...

https://en.wikipedia.org/wiki/Eosinophilia–myalgia_syndrome

... tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry ... Andersson B، Wentland MA، Ricafrente JY، وآخرون. (1996). "A "double adaptor" method for improved shotgun library construction ...

https://ar.wikipedia.org/wiki/ATXN2L

The spray is then ionised by extractive electrospray ionisation (EESI) and analysed using tandem mass spectrometry (MS/MS). An ... and its metabolite melamine in chard samples by ion-pair liquid chromatography coupled to electrospray tandem mass spectrometry ... However, electrospray ionization methods coupled with mass spectrometry allow a rapid and direct analysis of samples with ... The existing methods for melamine determination using a triple quadrupole liquid chromatography - mass spectrometry (LC/MS) ...

https://en.m.wikipedia.org/wiki/Melamine

Since then tandem mass spectrometry, gas chromatography-mass spectrometry , and DNA analysis has made it possible for a much ... Simonsen, H (25 November 2002). "[Screening of newborns for inborn errors of metabolism by tandem mass spectrometry]". ... Tandem mass spectroscopy can also be used for IEM, and investigation of sudden infant death, and shaken baby syndrome.[82] ... Cui, W; Ma, C. X.; Tang, Y; Chang, V; Rao, P. V.; Ariet, M; Resnick, M. B.; Roth, J (2005). "Sex differences in birth defects: ...

https://en.wikipedia.org/wiki/Congenital_disorder

"Quantification of chlordesmethyldiazepam by liquid chromatography-tandem mass spectrometry: application to a cloxazolam ... and determination of benzodiazepines in whole blood using solid-phase extraction and gas chromatography/mass spectrometry". ...

https://en.wikipedia.org/wiki/Cloxazolam

Many mass spectrometers use two or more mass analyzers for tandem mass spectrometry (MS/MS). In addition to the more common ... Main article: Ion-mobility spectrometry-mass spectrometry. Ion mobility spectrometry-mass spectrometry (IMS/MS or IMMS) is a ... accelerator mass spectrometry (AMS), thermal ionization-mass spectrometry (TIMS) and spark source mass spectrometry (SSMS). ... Another type of tandem mass spectrometry used for radiocarbon dating is accelerator mass spectrometry (AMS), which uses very ...

https://en.m.wikipedia.org/wiki/Mass_spectrometer

Wiens MD, Shen Y, Li X, Salem MA, Smisdom N, Zhang W, Brown A, Campbell RE (December 2016). "A Tandem Green-Red Heterodimeric ... Peptide mass fingerprinting/Protein mass spectrometry. *Dual-polarization interferometry. *Microscale thermophoresis. * ... Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW, Royant A (2012). " ... Baker SS, Vidican CB, Cameron DS, Greib HG, Jarocki CC, Setaputri AW, Spicuzza CH, Burr AA, Waqas MA, Tolbert DA (2012-01-01). ...

https://en.wikipedia.org/wiki/Green_fluorescent_protein

Nirogi et al.[5] reported a liquid chromatographic method coupled to tandem mass spectrometry for the quantification of ... "Quantification of Metaxalone in Human Plasma by Liquid Chromatography Coupled to Tandem Mass Spectrometry". J Anal Toxicol. 30 ...

https://en.wikipedia.org/wiki/Metaxalone

... long-term outcome and effects of expanded newborn screening using tandem mass spectrometry". J Inherit Metab Dis. 29 (2-3): 383 ...

https://en.wikipedia.org/wiki/Methylmalonic_acidemia

... by dispersive solid-phase extraction coupled with ultra-performance liquid chromatography/Tandem orbitrap mass spectrometry". ... Rapid Communications in Mass Spectrometry. 32 (20): 1791-1798. Bibcode:2018RCMS...32.1791Z. doi:10.1002/rcm.8222. PMID 29964303 ... Journal of the American Society for Mass Spectrometry, Volume 18, Issue 10, October 2007, Pages 1774-1782 ... Determination of Pyridine Alkaloids in Tobacco by PFTBA Ions/Analyte Molecule Reaction Ionization Ion Trap Mass Spectrometry, ...

https://en.wikipedia.org/wiki/Nicotiana_tabacum

https://en.wikipedia.org/wiki/PKP4

... polyphenol components using liquid chromatography-tandem mass spectrometry: Overall contribution to antioxidant activity". Food ...

https://zh.m.wikipedia.org/wiki/野櫻莓

Covey TR, Lee ED, Henion JD "High-speed liquid chromatography/tandem mass spectrometry for the determination of drugs in ... Covey TR, JB Crowther, EA Dewey, JD Henion "Thermospray liquid chromatography/mass spectrometry determination of drugs and ...

https://sr.wikipedia.org/wiki/Farmakokinetika

Tandem mass spectrometry is usually employed for added specificity. Standard curves and internal standards are used for ... Mass spectrometryEdit. Pharmacokinetics is often studied using mass spectrometry because of the complex nature of the matrix ( ... Covey TR, Lee ED, Henion JD (October 1986). "High-speed liquid chromatography/tandem mass spectrometry for the determination of ... There is currently considerable interest in the use of very high sensitivity mass spectrometry for microdosing studies, which ...

https://en.m.wikipedia.org/wiki/Pharmacokinetics

Tandem mass spectrometry, on the other hand, can get sequence information from individual peptides by isolating them, colliding ... Mass spectrometryEdit. Mass spectrometry has augmented proteomics.[6] Peptide mass fingerprinting identifies a protein by ... "Mass-Spectrometry-Based Draft of the Human Proteome". Nature.. *^ Kim, Min-Sik; et al. (May 2014). "A draft map of the human ... This map was generated using high-resolution Fourier-transform mass spectrometry. This study profiled 30 histologically normal ...

https://en.m.wikipedia.org/wiki/Proteome

... adenine dinucleotide and related compounds by liquid chromatography/electrospray ionization tandem mass spectrometry». Anal. ... Ma B, Pan SJ, Zupancic ML, Cormack BP «Assimilation of NAD(+) precursors in Candida glabrata». Mol. Microbiol., 66, 1, 2007, ... laboradors al Massachusetts Institute of Techonolgy el 2000) de les proteïnes diacilcetases dependents de NAD anomenades ...

https://ca.wikipedia.org/wiki/Nicotinamida_adenina_dinucleòtid

2005). "Quantification of docetaxel and its main metabolites in human plasma by liquid chromatography/tandem mass spectrometry ... Mass Spectrom. 19 (17): 2419-26. Bibcode:2005RCMS...19.2419G. doi:10.1002/rcm.2072. PMID 16059877.. ... Yvon AM, Wadsworth P, Jordan MA (April 1999). "Taxol Suppresses Dynamics of Individual Microtubules in Living Human Tumor Cells ...

https://en.wikipedia.org/wiki/Docetaxel

Gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) does not distinguish ... High-resolution mass spectrometry facilitated the development of multicomponent analytical methods for 150 pesticide ... The approach requires a relatively high amount of substance for gas chromatography-isotope ratio mass spectrometry (GC-IRMS) or ... Carbon 14-labeled pesticides do enable mass balances, but investigations with radioactively tagged substrates cannot be ...

https://en.wikipedia.org/wiki/Pesticide_degradation

"Multiplexed and data-independent tandem mass spectrometry for global proteome profiling". Mass Spectrometry Reviews. 33 (6): ... Targeted mass spectrometry. References[edit]. *^ Doerr, Allison (2014). "DIA mass spectrometry". Nature Methods. 12 (1): 35-35 ... precursor ion mass spectra whereas the high-energy CID is used to obtain product ion information by tandem mass spectrometry.[5 ... Tandem mass spectra are acquired either by fragmenting all ions that enter the mass spectrometer at a given time (called ...

https://en.wikipedia.org/wiki/Data-independent_acquisition

... adenine dinucleotide and related compounds by liquid chromatography/electrospray ionization tandem mass spectrometry". Anal. ... Ma B, Pan SJ, Zupancic ML, Cormack BP (2007). "Assimilation of NAD(+) precursors in Candida glabrata". Mol. Microbiol. 66 (1): ... descubrisen no Instituto de Tecnoloxía de Massachusetts (MIT) as proteínas chamadas sirtuínas; que son encimas deacetilases ...

https://gl.wikipedia.org/wiki/NADH

... milk using automated on-line column-switching-high performance liquid chromatography-isotope dilution tandem mass spectrometry ... 同時用母乳哺育二個不是雙胞胎或多胞胎的嬰兒的作法稱為協力車母乳哺育（tandem nursing）。每個嬰兒的胃口和吃母乳的習慣不同，因此可能是在不同的時間吃母乳，也可能是同時吃母乳，

https://zh.wikipedia.org/wiki/母乳餵養

... monitoring are most often performed through measurement of white blood cell cystine level using tandem mass spectrometry. ... Park MA,Thoene JG. Potential role of apoptosis in development of the cystinotic phenotype. Pediatr Nephrol 2005;20:441-446. ...

https://en.wikipedia.org/wiki/Cystinosis

... tandem mass spectrometry (LC-MS/MS), and other analytical methods, revealed a range from 48% to 80% of selected product samples ... "Determination of aflatoxins in commercial nuts and nut products using liquid chromatography tandem mass spectrometry". World ...

https://en.wikipedia.org/wiki/Aflatoxin

4-DMAA in Geranium Plants Using High Performance Liquid Chromatography with Tandem Mass Spectrometry at ng/g Concentrations". ... Methylhexanamine may be quantified in blood, plasma, or urine by gas or liquid chromatography-mass spectrometry to confirm a ...

https://en.wikipedia.org/wiki/Methylhexanamine

... now a commonly used technique for large molecules and routine liquid chromatography-tandem mass spectrometry. Early in his ... the American Society for Mass Spectrometry presented him with their Award for Distinguished Contributions in Mass Spectrometry ... Fenn shared half of the award with Koichi Tanaka for their work in mass spectrometry. The other half of the 2002 award went to ... He was semi-retired when he first published his research on electrospray ionization for mass spectrometry.[5] Fenn felt that ...

https://en.wikipedia.org/wiki/John_Bennett_Fenn

"Determination of picamilon concentration in human plasma by liquid chromatography-tandem mass spectrometry". Journal of ...

https://en.wikipedia.org/wiki/Picamilon

... Rapid Commun ...

https://pubmed.ncbi.nlm.nih.gov/22847692/?dopt=Abstract

Performance Metrics for Liquid Chromatography-Tandem Mass Spectrometry Systems in Proteomics Analyses. Paul A. Rudnick, Karl R ... Performance Metrics for Liquid Chromatography-Tandem Mass Spectrometry Systems in Proteomics Analyses ... Performance Metrics for Liquid Chromatography-Tandem Mass Spectrometry Systems in Proteomics Analyses ... Performance Metrics for Liquid Chromatography-Tandem Mass Spectrometry Systems in Proteomics Analyses ...

https://www.mcponline.org/content/9/2/225/tab-e-letters

Simultaneous determination of beta-blockers in human plasma using liquid chromatography-tandem mass spectrometry.. [Hironobu ... tandem mass spectrometry (MS-MS) using an MSpak GF column, which enables direct injection of crude plasma samples, is presented ...

https://www.sigmaaldrich.com/catalog/papers/18318013

... request pricing from manufacturers of Tandem Mass Spectrometers (MS/MS) , Mass Spectrometry ... Xevo TQ-S tandem quadrupole mass spectrometer - designed for the most demanding quantitative UPLC/MS/MS applications. The ... MALDI SYNAPT G2-Si High Definition Mass Spectrometry. Waters. Redefining Resolution - Resolution in 3 Dimensions. MALDI SYNAPT ... The Waters SYNAPT G2-S i High Definition Mass Spectrometry provides complete characterization of complex mixtures and molecules ...

https://www.selectscience.net/mass-spectrometry/product-directory/tandem-mass-spectrometers-

Analysis of isobars and isomers by tandem mass spectrometry is complicated by the similarity between their mass spectra and ... Tandem mass spectrometry is attractive for many applications due to its high selectivity, wide dynamic range, and high ... Analysis of isobars and isomers by tandem mass spectrometry is complicated by the similarity between their mass spectra and ... Tandem mass spectrometry is attractive for many applications due to its high selectivity, wide dynamic range, and high ...

https://www.ideaconnection.com/patents/8635-Quantitative-Analysis-of-Unresolved-Isomers-and-Isoba.html?c=6

... Kushnir, Mark M. ARUP ... Liquid chromatography tandem mass spectrometry is one of the most specific techniques available in clinical laboratories. In ... Steroids, Adrenal steroids, Androgens, Estrogens, Reference intervals, Tanner stage, Mass spectrometry National Category ...

http://uu.diva-portal.org/smash/record.jsf?pid=diva2:399245

Detection was carried out using Waters Micromass® Quattro Premier mass spectrometer in multiple reaction monitoring (MRM) mode ... sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MSMS) method for the determination of R-acenocoumarol ... A stereoselective, sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MSMS) method for the determination of ... Salem, I. , Abdullah, M. and Najib, N. (2015) Stereoselective High-Performance Liquid Chromatography Tandem Mass Spectrometry ...

http://scirp.org/journal/paperabs.aspx?paperid=53725

Analysis of Fusarium Toxins in Processed Grain Products Using High-Performance Liquid Chromatography/Tandem Mass Spectrometry ... and quantified using high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS). With wheat flour, the proposed ...

https://www.jstage.jst.go.jp/article/jpchrom/37/2/37_2016.003/_article

A liquid chromatography tandem mass spectrometry method was developed for quantifying ten cannabinoids in oral fluid (OF). This ... Title: Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid.. ... Abstract: A liquid chromatography tandem mass spectrometry method was developed for quantifying ten cannabinoids in oral fluid ... Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid. ...

https://www.cmcr.ucsd.edu/index.php/news-2/homeblog/225-validation-of-a-liquid-chromatography-tandem-mass-spectrometry-method-for-analyzing-cannabinoids-in-oral-fluid

Mass spectrometry/mass spectrometry: techniques and applications of tandem mass spectrometry. New York, N.Y: VCH Publishers. ... Tandem mass spectrometry, also known as MS/MS or MS2, involves multiple steps of mass spectrometry selection, with some form of ... The resulting ions are then separated and detected in a second stage of mass spectrometry (MS2). For tandem mass spectrometry ... Sleno L, Volmer DA (October 2004). "Ion activation methods for tandem mass spectrometry". Journal of Mass Spectrometry. 39 (10 ...

https://en.wikipedia.org/wiki/Tandem_mass_spectrometry

... tandem mass spectrometry, not tandem mass spectroscopy. * The standard abbreviation for tandem mass spectrometry is MS/MS, not ... of genetic disorders in human blood and urine using tandem mass spectrometry with liquid secondary ion mass spectrometry. ... atom bombardment with tandem mass spectrometry and liquid chromatography/mass spectrometry to the analysis of acylcarnitines in ... Automated tandem mass spectrometry for mass newborn screening for disorders in fatty acid, organic acid, and amino acid ...

https://www.cdc.gov/mmwr/preview/mmwrhtml/rr5003a1.htm

Tandem parallel fragmentation of peptides for mass spectrometry.. Ramos AA1, Yang H, Rosen LE, Yao X. ... of tandem mass spectrometers are sequentially combined to develop parallel collision-induced-dissociation mass spectrometry ( ... We implement the method using a quadrupole time-of-flight tandem mass spectrometer. The instrument is operated in TOF-MS mode ... p2CID MS). Compared to MS/MS spectra, the p2CID mass spectra show increased signal intensities (2-400-fold) and number of ...

https://www.ncbi.nlm.nih.gov/pubmed/16970313?dopt=Abstract

We applied TagGraph to a published human proteomic dataset of 25 million mass spectra and tripled confident spectrum ... Although mass spectrometry is well suited to identifying thousands of potential protein post-translational modifications (PTMs ... Ma, B. et al. PEAKS: powerful software for peptide de novo sequencing by tandem mass spectrometry. Rapid Commun. Mass Spectrom. ... Na, S., Bandeira, N. & Paek, E. Fast multi-blind modification search through tandem mass spectrometry. Mol. Cell. Proteomics 11 ...

https://www.nature.com/articles/s41587-019-0067-5?error=cookies_not_supported&code=0dab2e2a-8720-48f9-a5ea-84304e1d183f

Mass spectrometry has become a key technology for modern large-scale protein sequencing. Tandem mass spectrometry, the process ... Decision tree-driven tandem mass spectrometry for shotgun proteomics.. Swaney DL1, McAlister GC, Coon JJ. ... Recent advances in mass spectrometry now permit two discrete, and complementary, types of peptide ion fragmentation: collision- ... The probability of either a ETD or CAD tandem MS event resulting in a high confidence sequence identification for peptide ...

https://www.ncbi.nlm.nih.gov/pubmed/18931669?dopt=Abstract

Fast Gas Chromatography with Tandem Mass Spectrometry Analysis of Selected Persistent Organic Pollutants and Organophosphorus ... "Fast Gas Chromatography with Tandem Mass Spectrometry Analysis of Selected Persistent Organic Pollutants and Organophosphorus ... A fast GC with tandem MS method was developed and validated for multiresidue determination of 95 chemical contaminants (24 ...

https://www.ingentaconnect.com/content/aoac/jaoac/2017/00000100/00000003/art00007

... followed by ion chromatography-tandem mass spectrometry (IC-MS/MS) determination. A triple stage quadrupole mass spectrometer, ... Rapid Determination of Perchlorate Anion in Foods by Ion Chromatography-Tandem Mass Spectrometry. * Share ... Rapid Determination of Perchlorate Anion in Foods by Ion Chromatography-Tandem Mass Spectrometry ... Mass Spectrometry Software: Micromass Masslynx™ version 3.5 Manchester, UK).. Sample Preparation for High Moisture Fruits and ...

https://www.fda.gov/food/chemicals/rapid-determination-perchlorate-anion-foods-ion-chromatography-tandem-mass-spectrometry

Ion-electron reaction based fragmentation methods (ExD) in tandem mass spectrometry (MS), such as electron capture dissociation ... of a new qQq-FTICR mass spectrometer for post-translational modification analysis and top-down tandem mass spectrometry of ... and can be easily identified using mass spectrometry (MS) [40]. Isomerization does not change the molecular mass of the protein ... 2.3 Mass Spectrometry. Most ECD experiments were performed on a custom built qQq-FTICR MS with a nanospray source and a 7 T ...

https://link.springer.com/article/10.1007%2Fs13361-010-0049-9

Schilling O., auf dem Keller U., Overall C.M. (2011) Protease Specificity Profiling by Tandem Mass Spectrometry Using Proteome- ... Craig, R., and Beavis, R. C. (2004) TANDEM: matching proteins with tandem mass spectra, Bioinformatics 20, 1466-1467.PubMed ... Protease Specificity Profiling by Tandem Mass Spectrometry Using Proteome-Derived Peptide Libraries. ... and identified by liquid chromatography-tandem mass spectrometry. The corresponding nonprime-side sequences are derived ...

https://link.springer.com/protocol/10.1007/978-1-61779-148-2_17

Generating Peptide Sequence Tags for Peptide Identification via Tandem Mass Spectrometry ... TANDEM: matching proteins with tandem mass spectra by Robertson Craig, Ronald C. Beavis - Bioinformatics , 2004 ... Summary: Tandem mass spectra obtained from fragmenting peptide ions contain some peptide sequence specific information, but ... Summary: Tandem mass spectra obtained from fragmenting peptide ions contain some peptide sequence specific information, but ...

https://citeseerx.ist.psu.edu/search?q=Generating+Peptide+Sequence+Tags+for+Peptide+Identification+via+Tandem+Mass+Spectrometry

Newborn Screening by Tandem Mass Spectrometry, 2nd Edition, from CLSI. Browse this and other Newborn Screening standards in ...

https://clsi.org/standards/products/newborn-screening/documents/

Californias Experience Implementing a Pilot Newborn Supplemental Screening Program Using Tandem Mass Spectrometry. Lisa ... Californias Experience Implementing a Pilot Newborn Supplemental Screening Program Using Tandem Mass Spectrometry ... Newborn Screening for Isovaleric Acidemia Using Tandem Mass Spectrometry: Data from 1.6 Million Newborns ... Californias Experience Implementing a Pilot Newborn Supplemental Screening Program Using Tandem Mass Spectrometry ...

https://pediatrics.aappublications.org/content/117/Supplement_3/S261/tab-article-info

High Mass-Accuracy LC-MSMS Library The Wiley Registry of Tandem Mass Spectral Data: MS for ID contains 10,000 positive and ... The Wiley Registry of Tandem Mass Spectral Data: MS for ID comes bundled with one of the most accurate search algorithms ... Wiley Registry of Tandem Mass Spectral Data, MS for ID. Herbert Oberacher ... On the Development of a Robust and Transferable Tandem Mass Spectral Library for the Identification of Small Bioorganic ...

https://www.wiley.com/en-gb/Wiley+Registry+of+Tandem+Mass+Spectral+Data%2C+MS+for+ID-p-9781118037447

Antifungal Therapeutic Drug Monitoring by Liquid Chromatography Tandem Mass Spectrometry. *Are You Network Ready for the AACC ... Quantitation of azoles and echinocandins in compartments of peripheral blood by liquid -chromatography-tandem mass spectrometry ... Multiplex ultra-performance liquid chromatography-tandem mass spectrometry method for simultaneous quantification in human ... Antifungal Therapeutic Drug Monitoring by Liquid Chromatography Tandem Mass Spectrometry ...

https://www.aacc.org/Publications/CLN/Articles/2017/June/Antifungal-Therapeutic-Drug-Monitoring-by-Liquid-Chromatography-Tandem-Mass-Spectrometry.aspx

Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase ... Tandem mass spectrometry identifies many mouse brain O-GlcNAcylated proteins including EGF domain-specific O-GlcNAc transferase ... A method to form and detect low mass product ions in a quadrupole ion trap mass spectrometer. J Am Soc Mass Spectrom 17:81-84. ... detection and site-analysis of O-GlcNAc-modified glycopeptides by beta-elimination and tandem electrospray mass spectrometry. ...

https://www.pnas.org/content/109/19/7280?ijkey=7acb8fd4ef62399f662dc5fe724d6e25f976d054&keytype2=tf_ipsecsha

"Tandem mass spectrometry with collision induced dissociation isolates molecules of a single mass and then breaks them apart. ... The team used an analytical technique called tandem mass spectrometry-using an instrument provided by Lawrence Berkeley Lab and ... Sandia identifies unusual polycyclic aromatic hydrocarbons using tandem mass spectrometry. February 7, 2019 by Michael Padilla ... "Without the tandem component of this new mass spectrometer, each molecules mass is obtained but no information about its ...

https://phys.org/news/2019-02-sandia-unusual-polycyclic-aromatic-hydrocarbons.html

... to allow searching of tandem mass spectra for mass modifications. The search can consider any mass modification within a mass ... Most mass spectrometry search engines are reliable at matching peptide sequences to a significant number of tandem mass spectra ... In-depth Analysis of Tandem Mass Spectrometry Data from Disparate Instrument Types. Robert J. Chalkley, Peter R. Baker, Katalin ... in 55th ASMS Conference on Mass Spectrometry, Indianapolis, June 3-7, 2007, MPI 189, American Society for Mass Spectrometry, ...

https://www.mcponline.org/content/7/12/2386?ijkey=d8c08368e6fd974aacac30d8a7691ae17badc9e3&keytype2=tf_ipsecsha

Performance Metrics for Liquid Chromatography-Tandem Mass Spectrometry Systems in Proteomics Analyses. Paul A. Rudnick, Karl R ... Performance Metrics for Liquid Chromatography-Tandem Mass Spectrometry Systems in Proteomics Analyses ... 2004) TANDEM: matching proteins with tandem mass spectra. Bioinformatics 20, 1466- 1467. ... Performance Metrics for Liquid Chromatography-Tandem Mass Spectrometry Systems in Proteomics Analyses ...

https://www.mcponline.org/content/9/2/225?ijkey=8344d9c3e3a3d212a0112c72775396539cedb3f2&keytype2=tf_ipsecsha

... TAMS is a leading primary centre of research, training and service expertise in ...

http://tlabs.ac.za/?page_id=143

THPLC-Tandem Mass Spectrometry is a powerful analytical tool in order to define the fingerprint of the molecular species of ... in the book "Tandem Mass Spectrometry - Applications and Principles" edited by Jeevan K. Prasain, ISBN 978-953-51-0141-3, ... You are in: Characterization of Phospholipid Molecular Species by Means of HPLC-Tandem Mass Spectrometry ... Characterization of Phospholipid Molecular Species by Means of HPLC-Tandem Mass Spectrometry ...

http://www.univpm.it/English/Engine/RAServePG.php/P/811410010400/T/Characterization-of-Phospholipid-Molecular-Species-by-Means-of-HPLC-Tandem-Mass-Spectrometry

Tandem mass spectrometry, which has been previously shown by the Turecek lab to be of use in both newborn screening and in ... The Clinical Diagnosis of Porphyrias by Tandem Mass Spectrometry. dc.contributor.advisor. Turecek, Frantisek. en_US. ...

https://digital.lib.washington.edu/researchworks/handle/1773/22494?show=full

What is Tandem mass spectrometry? Meaning of Tandem mass spectrometry as a finance term. What does Tandem mass spectrometry ... Definition of Tandem mass spectrometry in the Financial Dictionary - by Free online English dictionary and encyclopedia. ... tandem mass spectrometry, hyphenated separation techniques, organic mass spectrometry, inorganic mass spectrometry, structure ... The growth of mass spectrometry and more specifically liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) [2] ...

https://financial-dictionary.thefreedictionary.com/Tandem+mass+spectrometry

Using tandem affinity purification (TAP) and mass spectrometry we have identified several novel protein interactions with the ... we have established the AHR-PIN using tandem affinity purification (TAP) and mass spectrometry (MS). Here we report the AHR-PIN ... Gel Electrophoresis and Mass Spectrometry. Samples were separated on a 4-12% Bis-Tris gradient gel (NuPage, Invitrogen) by ... a) Venn diagram of proteins identified by mass spectrometry in 3 data sets of AHR-TAP 30 min TCDD (10 nM) dosing. (b) Table ...

https://www.hindawi.com/journals/jt/2013/279829/

... are normal phase and reversed phase separation strategies for fatty acyls that include detection via tandem mass spectrometry. ... are normal phase and reversed phase separation strategies for fatty acyls that include detection via tandem mass spectrometry. ... Common analytical methodologies involve chromatographic separation and mass spectrometric techniques; however, a single step is ... Common analytical methodologies involve chromatographic separation and mass spectrometric techniques; however, a single step is ...

https://www.frontiersin.org/articles/10.3389/fphys.2016.00608/full

Measurement of trimethylamineNoxide by stable isotope dilution liquid chromatography tandem mass spectrometry.. Posted on March ... Measurement of trimethylamineNoxide by stable isotope dilution liquid chromatography tandem mass spectrometry. ...

http://www.strokecenter.org/measurement-of-trimethylaminenoxide-by-stable-isotope-dilution-liquid-chromatography-tandem-mass-spectrometry/

SS: Why is tandem mass spectrometry, LC-MS/MS so important to the work being conducted in your laboratory? ... Tandem mass spectrometry is indispensable to our high-throughput screening program, which covers the analysis of approximately ... Editorial Article: Enhancing the Lives of Children with Tandem Mass Spectrometry. How scientists at Birmingham Womens and ... We wouldnt be able to analyze the quantity of samples that meets the quality of national guidelines without these tandem mass ...

https://www.selectscience.net/editorial-articles/selectscience/enhancing-the-lives-of-children-with-tandem-mass-spectrometry/?artID=46766

Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Tyrosine - analogs & derivatives - analysis - metabolism ... Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Abstract. Analytical methods are generally developed and ... Tandem Mass Spectrometry - methods Waste Water - analysis - chemistry Water Pollutants, Chemical - analysis Water Purification ... Tandem Mass Spectrometry Thyrotropin - blood Young Adult Abstract. Perfluoroalkyl substances (PFASs) are a group of highly ...

https://arctichealth.org/en/list?q=topic%3A%22Tandem+Mass+Spectrometry%22&p=1&ps=&sort=title_sort+asc

Liquid Chromatography/Tandem Mass Spectrometry for the Simultaneous Determination of Alverine and its Metabolite, Monohydroxy ... "Liquid Chromatography/Tandem Mass Spectrometry for the Simultaneous Determination of Alverine and its Metabolite, Monohydroxy ...

https://www.hindawi.com/journals/jchem/2011/109414/cta/

Liquid Chromatography and Tandem Ion Trap-Mass Spectrometry. Maryam Khaksari1, Lynn R. Mazzoleni2, Chunhai Ruan3, Peng Song4, ... and Fat-Soluble Vitamins with Liquid Chromatography and Tandem Ion Trap-Mass Spectrometry. ... with a C18 reversed-phase column and an ion trap mass spectrometry (MS) detector coupled with an electrospray ionization (ESI) ...

https://www.aiche.org/conferences/aiche-annual-meeting/2014/proceeding/paper/simultaneous-detection-and-quantification-water-and-fat-soluble-vitamins-liquid-chromatography-and-1

Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has become one of the most used tools in mass spectrometry based proteomics. (biomedcentral.com)
In shotgun proteomics, peptides are separated using liquid chromatography and introduced into a mass spectrometer via an ionization interface. (biomedcentral.com)
Peptide identification via tandem mass spectrometry sequence database searching is a key method in the array of tools available to the proteomics researcher. (broadinstitute.org)
The ability to rapidly and sensitively acquire tandem mass spectrometry data and perform peptide and protein identifications has become a commonly used proteomics analysis technique because of advances in both instrumentation and software. (broadinstitute.org)

Address the most stringent analytical challenges for targeted quantitation workflows with the Thermo Scientific™ TSQ Altis™ Triple Quadrupole Mass Spectrometer. (selectscience.net)
Identify and quantify more proteins, peptides, lipids, glycans and small molecules accurately and in less time with the Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer. (selectscience.net)
Cannabinoids were separated by liquid chromatography over a BEH C18 column and detected by a Waters TQ-S micro tandem mass spectrometer. (ucsd.edu)
The model is rigorously derived and sensitive to the searching tolerance determined by the accuracy of mass spectrometer. (biomedcentral.com)
Tandem mass spectrometry (MS-MS) is a related technology in which compounds are separated by molecular weight by one mass spectrometer. (cclluueess.com)
The simplest form of tandem mass spectrometry combines two Simple Schematic of a Two Mass Spectrometer Tandem MS/MS Mass Spectrometry - Essays and Tutorials Mini-Tutorial: Understanding Mascot Reports (MS/MS Ion Search Results) data from tandem mass spectrometry experiments. (cclluueess.com)

A detailed procedure for the analysis of four beta-blockers, acebutolol, labetalol, metoprolol and propranolol, in human plasma by high-performance liquid chromatography (LC)-tandem mass spectrometry (MS-MS) using an MSpak GF column, which enables direct injection of crude plasma samples, is presented. (sigmaaldrich.com)
Baker, Sharyn D. / Quantification of sunitinib in human plasma by high-performance liquid chromatography-tandem mass spectrometry . (elsevier.com)
Determination of pharmaceuticals and personal care products in fish using high performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry. (tdl.org)

The sensitivity, selectivity, flexibility, and ease-of-use provided by hybrid quadrupole-Orbitrap mass spectrometers set the standard for screening, quantitation, identification, and confirmation of targeted and untargeted compounds. (selectscience.net)
This webinar will present research on different liquid chromatography tandem mass spectrometry testosterone analytical methods that can be performed on low-, mid- and high-sensitivity mass spectrometers. (labroots.com)
these analyzers can also be combined to form tandem mass spectrometers. (cclluueess.com)

Solid phase extraction was used for sample preparation, followed by liquid chromatography tandem mass spectrometry analysis. (revistadechimie.ro)

Sample preparation followed by GC or HPLC separation and mass spectrometry (MS) detection has become the standard approach for evaluating PPCPs in environmental samples. (tdl.org)

Analysis of isobars and isomers by tandem mass spectrometry is complicated by the similarity between their mass spectra and often requires extensive chromatographic separation in order to eliminate mutual interference, and many methods for the separation of structural isomers are not compatible for quantitative analysis. (ideaconnection.com)
All tandem MS spectra in the data set are then compared with the theoretical spectra [ 1 ]. (biomedcentral.com)
OMSSA, X!Tandem and Crux were applied to identify simulated mass spectra on a database of 7857 mouse neuropeptides from 92 prohormones. (illinois.edu)

Dr. Hayden is a board certified clinical chemist with a special interest in the development and implementation of new diagnostics, in particular mass spectrometry-based assays for small molecule analysis. (labroots.com)
This has led many clinical societies to advocate for research into mass spectrometry-based testing. (labroots.com)
Given this recommendation, and the often high volume of testing, many clinical laboratories are considering the development of their own in-house mass spectrometry-based testosterone analytical methods. (labroots.com)

We describe here a sensitive and rapid testosterone assay based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) utilizing on-line sample extraction and multiplexing. (elsevier.com)
Objective To determine whether liquid chromatography-tandem mass spectrometry (LC-MS/MS) determination of total (TT) and free (FT) testosterone is more specific than extraction chromatography-radioimmunoassay (RIA) for distinguishing women with polycystic ovary syndrome (PCOS) from controls and whether differing cutoff values should be used depending on the setting. (elsevier.com)

A method for simultaneous determination of metribuzin (MET) and three metribuzin metabolites in livestock products and seafoods by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. (elsevier.com)

Simultaneous determination of beta-blockers in human plasma using liquid chromatography-tandem mass spectrometry. (sigmaaldrich.com)

2004) Simultaneous quantitative analysis of isobars by tandem mass spectrometry from unresolved chromatographic peaks. (ideaconnection.com)

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a well-established tool for the identification and quantification of small molecules in research and industrial settings, but the technique has only recently moved into the healthcare arena. (spectroscopyeurope.com)

Tools to identify proteins in tandem mass spectrometry experiments are not optimized to identify neuropeptides due to complex processing, post-translational modifications and neuropeptide size. (illinois.edu)

The analytes were monitored by tandem-mass spectrometry with electrospray positive ionization. (elsevier.com)

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful technique for the quantification of small molecules in body fluids, but some sample preparation is necessary prior to analysis. (spectroscopyeurope.com)
The Crux mass spectrometry analysis toolkit is an open source project that aims to provide users with a cross-platform suite of analysis tools for interpreting. (cclluueess.com)
Introduction to MS Quantitation This tutorial applies to the quantitation of small molecules using mass spectrometry in pharmacokinetic analysis. (cclluueess.com)
Chiriac FL, Paun I, Pirvu F, Pascu LF, Niculescu M, Galaon T. Liquid Chromatography Tandem Mass Spectrometry Method for Ultra-Trace Analysis of Organic UV Filters in Environmental Water Samples. (revistadechimie.ro)
Liquid Chromatography Tandem Mass Spectrometry Method for Ultra-Trace Analysis of Organic UV Filters in Environmental Water Samples', Revista de Chimie, 71(1), pp. 92-99. (revistadechimie.ro)
Chambliss, C. K. "Analysis of pharmaceuticals in fish using liquid chromatography-tandem mass spectrometry. (tdl.org)

Although many different tandem mass spectrometry database search tools are currently available from both academic and commercial sources, these algorithms share similar core elements while maintaining distinctive features. (broadinstitute.org)

The method can be adapted to any tandem mass spectroscopy platform. (ideaconnection.com)
Tandem Mass Spectroscopy Basics 1. (cclluueess.com)

A probability based statistical scoring model for assessing peptide and protein matches in tandem MS database search was derived. (biomedcentral.com)
Protein ID With MS/MS Data Mass Spectrometry Resource. (cclluueess.com)

Journal of Mass Spectrometry 39:532-40. (ideaconnection.com)

Tandem parallel fragmentation of peptides for mass spectrometry. (nih.gov)
Parallel fragmentations of peptides in the source region and in the collision cell of tandem mass spectrometers are sequentially combined to develop parallel collision-induced-dissociation mass spectrometry (p2CID MS). Compared to MS/MS spectra, the p2CID mass spectra show increased signal intensities (2-400-fold) and number of sequence ions. (nih.gov)
A statistical model is presented for computing probabilities that proteins are present in a sample on the basis of peptides assigned to tandem mass (MS/MS) spectra acquired from a proteolytic digest of the sample. (nih.gov)
Primary amines of all peptides are then chemically protected so that after incubation with a test protease, the neo-N-termini of the prime-side cleavage products with exposed α-amines can be specifically biotinylated, enriched, and identified by liquid chromatography-tandem mass spectrometry. (springer.com)
Immobilized metal affinity chromatography in combination with tandem mass spectrometry was utilized to identify 19 phosphorylation sites on the five major H1 isoforms plus H1.X. Fourteen of these phosphorylation sites were located on peptides containing the cyclin dependent kinase (CDK) consensus motif (S/T)-P-X-Z (where X is any amino acid and Z is a basic amino acid). (nih.gov)
While electron transfer dissociation (ETD) has been shown to outperform collision-induced dissociation (CID) in sequencing glycated peptides by tandem mass spectrometry, ETD instrumentation is not yet available in all laboratories. (unt.edu)
Quantification of serum IgG subclasses by use of subclass-specific tryptic peptides and liquid chromatography-tandem mass spectrometry. (thefreelibrary.com)
The use of proteotypic peptides along with liquid chromatography-tandem mass spectrometry (LC-MS/ MS), a technique that does not suffer from antigen excess, has emerged as a useful tool for quantifying proteins (9-12). (thefreelibrary.com)
The article discusses a study which quantified multiple amyloid beta peptides in human cerebrospinal fluid (CSF) for preclinical or biomarker discovery using an improved solid-phase extraction-ultraperformance liquid chromatography-mass spectrometry-mass spectrometry (SPE-UPLC-MS-MS). (ebscohost.com)

Several modifications never previously reported in proteomics data were identified in these standard data sets using this mass modification searching approach. (mcponline.org)
There are many search engines available to the researcher for the analysis of proteomics data produced by tandem mass spectrometry. (mcponline.org)

Sandia National Laboratories scientists Scott Skeen, left, Nils Hansen, center, and Brian Adamson discuss tandem mass spectrometry, which was used for the detection of aliphatically linked polycyclic aromatic hydrocarbons found in soot producing flames. (phys.org)
As well as searching for known modification types, Protein Prospector permits the detection and identification of unexpected or novel modifications by searching for any mass shift within a user-specified mass range to any chosen amino acid(s). (mcponline.org)
Described in this manuscript are normal phase and reversed phase separation strategies for lipids that include detection of the bioactive primary fatty acid amides and N-acyl glycines via tandem mass spectrometry. (frontiersin.org)
1.1 This procedure covers the determination of selected polyfluorinated compounds (PFCs) in a soil matrix using solvent extraction, filtration, followed by liquid chromatography (LC) and detection with tandem mass spectrometry (MS/MS). These analytes are qualitatively and quantitatively determined by this method. (astm.org)
1.1 This test method covers the determination of dioctyl sulfosuccinate (DOSS) in sea water by direct injection using liquid chromatography (LC) and detection with tandem mass spectrometry (MS/MS). This analyte is qualitatively and quantitatively determined by this test method. (astm.org)
In a previous work, coupled-column LC (LC/LC) with direct large-volume sample injection for the analysis of beta 2-agonists in bovine urine was investigated, in view of its combination with thermospray (TSP) tandem mass spectrometric (MS/MS) detection. (unboundmedicine.com)
For the first time a quantitative method for the analysis of trace levels of eight chloramphenicol isomers in urine by chiral liquid chromatography in combination with tandem mass spectrometric detection is reported. (wur.nl)
Lamotrigine was extracted from plasma through a simple protein precipitation and analyzed by fast liquid chromatography coupled to heated electrospray ionization with tandem mass spectrometric detection. (ovid.com)
The method consists of a rapid protein precipitation by methanol followed by high throughput online solid phase extraction (SPE), ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS), and negative electrospray ionization detection. (uio.no)
Differential mobility spectrometry (DMS) has the potential to improve the performance of LC-MS methods for PSTs in terms of selectivity and limits of detection. (springer.com)
The determination of lipoxin A(4) has been accomplished by chromatographic separation using a C18 reversed phase column and tandem mass spectrometry detection. (fraunhofer.de)
Hereafter, Adding to an improvement on detection accuracy of the heavier element, we are planning to measure 129I with di-molecular pilot beam method as a very important elements especially on our large tandem accelerator. (nii.ac.jp)
A 2 μL aliquot of this extract was injected onto a C 18 ‐column, gradient elution was applied and triple‐quadrupole mass spectrometry in positive‐ion mode was used for detection. (separationsnow.com)

Increasingly, tandem mass spectrometry (MS/MS) is being used for newborn screening because this laboratory testing technology substantially increases the number of metabolic disorders that can be detected from dried blood-spot specimens. (cdc.gov)
The introduction of tandem mass spectrometry (MS/MS) in the 1990s for population-based newborn screening has enabled health-care providers to detect an increased number of metabolic disorders in a single process by using dried blood-spot specimens routinely collected from newborns ( 13 ). (cdc.gov)
Tandem mass spectrometry (TMS) and T-cell-receptor excision circles (TRECs) can identify early-onset ADA deficiency at birth, on dried blood spot (DBS) taken within the routine newborn screening procedure. (aaaai.org)
Tandem mass spectrometry, which has been previously shown by the Turecek lab to be of use in both newborn screening and in clinical diagnostics, has the potential to be used in the diagnosis of the porphyrias, as the sensitivity and selectivity of the technique can be utilized to develop more efficient and effective assays than those that currently exist. (washington.edu)
State Newborn Screening in the Tandem Mass Spectrometry Era: More False-Positive Results. (thefreedictionary.com)
Tandem Mass Spectrometry QA/QC for Newborn Screening: Routine Operations. (slideserve.com)

A simple and fast method for analysis of hydroxylated polycyclic aromatic hydrocarbons using pressurized liquid extraction and high performance liquid chromatography utilizing photoionization tandem mass spectrometry was developed. (diva-portal.org)
Accordingly, this study shows that high performance liquid chromatography photoionization tandem mass spectrometry can be a good option for the determination of hydroxylated polycyclic aromatic hydrocarbons in complex environmental samples. (diva-portal.org)
The method is based on liquid chromatography/atmospheric pressure photoionization tandem mass spectrometry (LC/APPI-MS/MS). Validation was provided in smoked cigarette filter. (spectroscopynow.com)

Electrospray mass spectrometIy has aided the structural characterization of ecdysteroid moulting hormones as a means of understanding factors controlling the moulting cycle in crabs. (hindawi.com)
Age-associated tyrosine nitration of rat skeletal muscle glycogen phosphorylase b: characterization by HPLC-nanoelectrospray-tandem mass spectrometry. (arctichealth.org)

This experiment is commonly performed to identify transitions used for quantification by tandem MS. In a neutral loss scan, the first mass analyzer scans all the masses. (wikipedia.org)
Although collision-induced dissociation (CID)-based tandem mass spectrometry (MS/MS) is commonly applied for identification and quantification of cystine in both biomedical and nutritional studies, gas-phase fragmentation reactions of cystine in CID has remained unclear. (mdpi.com)
Successful and cost-efficient replacement of immunoassays by tandem mass spectrometry for the quantification of immunosuppressants in the clinical laboratory. (thefreedictionary.com)
Liquid chromatography--tandem mass spectrometry work flow for parallel quantification of methotrexate and other immunosuppressants. (thefreelibrary.com)
A fast and easy-to-use liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination and quantification of a novel antifungal drug, olorofim (F901318), a member of the novel class of orotomides, in human plasma and serum was developed and validated. (asm.org)
A rapid and sensitive liquid chromatography/tandem mass spectrometric (LC-MS/MS) method for simultaneous quantification of fentanyl (F), norfentanyl (NF), despropionylfentanyl (DPF), and hydroxynorfentanyl (OHNF) in human plasma and urine specimens has been developed and validated according to. (ebscohost.com)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful technique for the quantification of small molecules in body fluids, but some sample preparation is necessary prior to analysis. (spectroscopyeurope.com)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a well-established tool for the identification and quantification of small molecules in research and industrial settings, but the technique has only recently moved into the healthcare arena. (spectroscopyeurope.com)
To support therapeutic drug monitoring of patients with cancer, a fast and accurate method for simultaneous quantification of the registered anticancer drugs afatinib, axitinib, ceritinib, crizotinib, dabrafenib, enzalutamide, regorafenib and trametinib in human plasma using liquid chromatography tandem mass spectrometry was developed and validated. (separationsnow.com)

Fast Gas Chromatography with Tandem Mass Spectrometry Analysis of. (ingentaconnect.com)
During the past 15 years, liquid chromatography tandem mass spectrometry (LC-MS/MS) has evolved into a vital technology used to perform routine tests in many clinical laboratories. (aacc.org)
Individual procedures for foods involve extraction, graphitized carbon solid phase extraction (SPE) cleanup, filtration, followed by ion chromatography-tandem mass spectrometry (IC-MS/MS) determination. (fda.gov)
The analyte is recovered from plasma or brain homogenate by liquid-liquid extraction and subsequently analyzed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). A simple experimental protocol renders the procedure valuable for obtaining information rapidly on brain penetration and plasma exposure of specific classes of compounds. (nih.gov)
Histone H1 isoforms isolated from asynchronously grown HeLa cells were subjected to enzymatic digestion and analyzed by nano-flow reversed-phase high performance liquid chromatography (RP-HPLC) tandem mass spectrometry (MS/MS) on both quadrupole ion trap and linear quadrupole ion trap-Fourier transform ion cyclotron resonance mass spectrometers. (nih.gov)
This review highlights the utility of antifungal TDM and explains how to perform testing using liquid chromatography tandem-mass spectrometry (LC-MS/MS). (aacc.org)
Liquid chromatography/tandem mass spectrometry study of forced degradation of azilsartan medoxomil potassium. (sigmaaldrich.com)
Simultaneous determination of beta-blockers in human plasma using liquid chromatography-tandem mass spectrometry. (sigmaaldrich.com)
A detailed procedure for the analysis of four beta-blockers, acebutolol, labetalol, metoprolol and propranolol, in human plasma by high-performance liquid chromatography (LC)-tandem mass spectrometry (MS-MS) using an MSpak GF column, which enables direct injection of crude plasma samples, is presented. (sigmaaldrich.com)
The growth of mass spectrometry and more specifically liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) [2] continues to accelerate in the clinical laboratory. (thefreedictionary.com)
Analytical methods that employ UltraPerformance Liquid Chromatography (UPLC(R)) coupled with tandem mass spectrometry can screen for more than 100 different drug residues at a time at concentrations of less than 10 ng/mL, a big improvement in speed, efficiency, and sensitivity over the traditional seven-plate bioassay method. (thefreedictionary.com)
Measurement of trimethylamineNoxide by stable isotope dilution liquid chromatography tandem mass spectrometry. (strokecenter.org)
The method uses liquid chromatography (LC) with a C18 reversed-phase column and an ion trap mass spectrometry (MS) detector coupled with an electrospray ionization (ESI) probe. (aiche.org)
This paper presents the development of a high-throughput and sensitive method for determining four vitamin D metabolites in dried blood spots using liquid chromatography coupled with tandem mass spectrometry. (rsc.org)
As an alternative to reagent-dependent proprietary methods, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, with an improved specificity, can be used to measure methotrexate (2,3). (thefreelibrary.com)
Gas chromatography coupled to mass spectrometry (GC/MS) has been extensively applied for determination of volatile, nonpolar, compounds in many applied fields like food safety, environment, or toxicology. (wur.nl)
Liquid chromatography tandem mass spectrometry is one of the most specific techniques available in clinical laboratories. (diva-portal.org)
A stereoselective, sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MSMS) method for the determination of R-acenocoumarol and S-acenocoumarol in human plasma was developed and validated at IPRC bioanalytical labs. (scirp.org)
Liquid chromatography coupled to mass spectrometry is now the reference method for small analyte determination. (archives-ouvertes.fr)
In this article, a bioanalytical method for the extraction and determination of inositol hexaphosphate in whole blood is described by using solid-phase extraction followed by liquid chromatography-mass spectrometry/mass spectrometry using selected reaction monitoring mode for its specificity. (archives-ouvertes.fr)
Here, we compare absolute and ranked concentrations of estrone, estradiol, and estriol measured by indirect RIA and of 2-hydroxyestrone and 16α-hydroxyestrone measured by ELISA to the concentrations obtained using a novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, which measures 15 estrogen metabolites concurrently. (aacrjournals.org)
Direct Quantitation of the Quorum Sensing Signal, Autoinducer-2, in Clinically Relevant Samples by Liquid Chromatography-Tandem Mass Spectrometry. (rti.org)
Herein we report a liquid chromatography−tandem mass spectrometric technique that enables reproducible, quantitative, and sensitive measurement of the concentration of autoinducer-2 from a variety of sources. (rti.org)
Hyphenation of coupled-column liquid chromatography and thermospray tandem mass spectrometry for the rapid determination of beta 2-agonist residues in bovine urine using direct large-volume sample injection. (unboundmedicine.com)
1.1 This procedure covers the determination of aldicarb, carbofuran, oxamyl and methomyl (referred to collectively as carbamates in this test method) in surface water by direct injection using liquid chromatography (LC) and detected with tandem mass spectrometry (MS/MS). These analytes are qualitatively and quantitatively determined by this method. (environmental-expert.com)
Since the introduction of Liquid Chromatography-Tandem Mass Spectrometry (LC/MS/MS) into the clinical research laboratory over a decade ago, it has widely become accepted as the technique of choice for specific and accurate quantitation of many clinically relevant molecules in complex biological matrices. (separationsnow.com)
The trace analysis of alkyl alkylphosphonic acids in urine using gas chromatography-ion trap negative ion tandem mass spectrometry. (biomedsearch.com)
We report here a powerful analytical method based on hydrophilic interaction liquid chromatography-electrospray ionization tandem mass spectrometry (HILIC-ESI-MS/MS), further tested on rat retina. (arvojournals.org)
A sensitive and specific ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for the measurement of climbazole deposition from hair care products onto artificial skin and human scalp. (uva.nl)
Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid. (ucsd.edu)
A liquid chromatography-heated electrospray ionization-tandem mass spectrometry method including a backflush step was developed and validated to measure lamotrigine concentration in patient plasma. (ovid.com)
Liquid chromatography (LC) coupled to mass spectrometry (MS) offers highly selective and sensitive analysis of a wide variety of compounds. (diva-portal.org)
Methods: Analysis was performed by liquid chromatography-tandem mass spectrometry in positive ionization mode. (ebscohost.com)
Determination of homocitrulline in urine of patients with HHH syndrome by liquid chromatography tandem mass spectrometry. (ebscohost.com)
A liquid chromatography tandem mass spectrometric method is described for the analysis of homocitrulline in human urine, a key metabolite in the differential diagnosis of hyperammonemia, hyperornithinemia, homocitrullinuria (HHH) syndrome. (ebscohost.com)
Hence, this thesis focuses on applying a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to measure BAs in blood samples collected from a bariatric surgery study and a gestational probiotic intervention study. (researchgateway.ac.nz)
Derivatized thiols were preconcentrated by online solid-phase extraction (SPE) followed by liquid chromatography separation and electrospray ionization tandem mass spectrometry determination (SPE/LC-ESI-MS/MS). The robustness of the method was validated for wide ranges in pH, salinity, and concentrations of sulfide and dissolved organic carbon (DOC) to cover contrasting natural water types. (diva-portal.org)
Using the bottom-up approach and liquid chromatography (LC) in combination with mass spectrometry, the primary structure and sequence microheterogeneity of a plaque-specific anti-Î²-amyloid (1â€"17) monoclonal antibody (clone 6E10) was characterized. (ebscohost.com)
The extraction procedure is very simple and steroid separation is ensured on a BEH C18 column and an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Analysis was done in positive ionization mode (ESI+) and recorded in multiple reaction monitoring mode (MRM). (ovid.com)
The sample was directly injected into the turbulent flow liquid chromatography tandem mass spectrometry system (TFLC-MS/MS) for online extraction followed by HPLC separation. (omicsonline.org)
Measurement of Pancreatic Polypeptide and Its Peptide Variant in Human Serum and Plasma by Immunocapture-Liquid-Chromatography-Tandem Mass Spectrometry. (omicsonline.org)
We developed an Immunocapture-Liquid Chromatography- Tandem Mass Spectrometry (IC-LC-MS/MS) assay to quantify Pancreatic Polypeptide (PP) and its metabolite, PP3-36, in human serum or plasma. (omicsonline.org)
To overcome this limitation, drug metabolism scientists predict likely metabolic transformations and use full-scan liquid chromatography with mass spectrometry (LC-MS) data to search for predicted products of metabolism ( Gibson and Skett, 1986 ). (aspetjournals.org)
Methods Serum from 183 women and 146 men was analyzed using equilibrium dialysis (ED), with FT directly measured by liquid chromatography-tandem mass spectrometry. (deepdyve.com)
Baiwei Lin and Joseph H. Pease, "A Novel Method for High Throughput Lipophilicity Determination by Microscale Shake Flask and Liquid Chromatography Tandem Mass Spectrometry", Combinatorial Chemistry & High Throughput Screening (2013) 16: 817. (eurekaselect.com)
The authors have developed a simple, sensitive and precise liquid-liquid extraction aldosterone method for the ABSCIEX API-5000 liquid chromatography and tandem mass spectrometry (LC-MS/MS) system. (bmj.com)

Guo, M. Studies on Transition Metal-Quercetin Complexes Using Electrospray Ionization Tandem Mass Spectrometry. (mdpi.com)
Liu Y, Guo M. Studies on Transition Metal-Quercetin Complexes Using Electrospray Ionization Tandem Mass Spectrometry. (mdpi.com)

During this presentation the basic principles of operation of the two most commonly used LC- Tandem Mass Spectrometry technologies, triple quadrupole and hybrid-triple quadrupole (QTRAP ® ) systems, will be discussed in terms how they are used to quantitate and /or confirm the identity of clinically relevant compounds in the clinical research laboratory. (separationsnow.com)

An accelerator mass spectrometry (AMS) system has been developed with a large 12UD tandem static accelerator as a three year project starting from 1992.A 'di-molecular pilot beam method' has been tested successfully and is used for AMS measurements without any modification to the existing tandem system. (nii.ac.jp)
Publications] Yasuo Nagashima: 'The Construction of an Accelerator Mass Spectrometry System with 12UD Pelletron Tandem Accelerator at the University of Tsukuba' Proceedings of the Fifth Japan-China Joint Symposium on Accelerator for Nuclear Science and Their Application. (nii.ac.jp)
Publications] Y.Nagashima, N.yoshizawa, H.shioya, T.Takahashi, T.Kaikura, Y.Tajima, T,Aoki, and K.Furuno: 'The Construction of an Accelerator Mass Spectrometry System with 12UD Pelletron Tandem Accelerator at the University of Tsukuba. (nii.ac.jp)

Each instrumental configuration utilizes a unique mode of mass identification. (wikipedia.org)
On the Development of a Robust and Transferable Tandem Mass Spectral Library for the Identification of Small Bioorganic Molecules. (wiley.com)
The instrument enables mass spectrum measurement for qualitative analysis or identification of unknowns and Selected Ion Monitoring (SIM) and MRM Measurement for quantitation of trace elements. (utm.my)
In this article, we describe the analytical methods for extraction, identification, and quantitation of sphingolipids using state-of-the-art tandem mass spectrometry (MS/MS). Precursor ion scans are used to distinguish various species of sphingolipids in crude extracts by their unique molecular decomposition products. (sciencemag.org)
10 ppm) mass measurement allowed identification of human glycoproteins and revealed differences in glycosylation. (lu.se)

The parallel approach using LC coupled to both inductively coupled plasma (ICP) mass spectrometry, and electrospray ionization (ESI) tandem MS is also evaluated as a tool to identify unknown siderophores in a sample. (diva-portal.org)
Over the last few decades, inductively coupled plasma mass spectrometry (ICP-MS) has been increasingly used in the nuclear sector as a rapid alternative to decay counting techniques for long-lived radionuclides, as well as an expanding range of shorter-lived radionuclides. (soton.ac.uk)
Warwick, Phillip , Russell, Benjamin , Croudace, Ian and Zacharauskas, Zilvinas (2019) Evaluation of inductively coupled plasma tandem mass spectrometry for radionuclide assay in nuclear waste characterisation. (soton.ac.uk)

Tandem mass spectrometry can be used to solve a number of protein structural problems that are not amenable to conventional methods for amino acid sequencing. (sciencemag.org)
Systems and methods are used to analyze a sample using variable mass selection window widths. (freepatentsonline.com)

The selection of the different mass selection window widths can be based on one or more properties of sample compounds. (freepatentsonline.com)
5. The system of claim 1, wherein the precursor mass selection window widths are based on one or more properties of sample compounds. (freepatentsonline.com)
The formation of a highly abundant (quasi) molecular ion was the main goal because of the enhanced selectivity when used as precursor ion in tandem MS. The addition of water as a modifier was tested to promote the generation of protonated molecules, resulting in notable improvement of sensitivity and selectivity for most compounds. (wur.nl)

The researchers used tandem mass spectrometry to retrospectively analyze dust samples taken from a 350- to 420-head pig-fattening farm from 1981 to 2000. (thefreedictionary.com)
Tandem mass spectrometry (MS/MS) has enabled research workers to analyze organic biological samples because the primary idea inception. (exposed-skin-care.net)

Nevertheless, the team maintains that these values could still provide an accurate mass measurement in MS2. (thermofisher.com)
This study evaluates the potential application of tandem ICP-MS/MS for the measurement of a range of radionuclides of interest to nuclear decommissioning and waste management. (soton.ac.uk)

This approach is a conceptual extension of tandem mass spectrometry in which interrogation of the full data domain is performed by simultaneous implementation of precursor and neutral loss scans. (rsc.org)

Judy Stone, who works for TPMG Kaiser Regional Laboratory, has been using tandem mass spectrometry technologies for years to conduct testing of fewer than 100 samples per day. (thefreedictionary.com)
As far as we know, hydroxylated polycyclic aromatic hydrocarbons were determined in wood smoke and soil samples using photoionization mass spectrometry for the first time in this present study. (diva-portal.org)

When using multiple quadrupoles, they can act as both mass analyzers and collision chambers. (wikipedia.org)
The method uses two mass filters arranged sequentially with a collision cell between them. (aacc.org)
Tandem mass spectrometry with collision induced dissociation isolates molecules of a single mass and then breaks them apart. (phys.org)

On the other hand, MS/MS has superior selectivity for many analytes since it recognizes them by at least two physical properties-their precursor and product ion mass. (aacc.org)
Tandem mass spectrometry is attractive for many applications due to its high selectivity, wide dynamic range, and high throughput capabilities. (ideaconnection.com)

Mass Spectrometry Conditions: For those using Waters Micromass equipment, we have found that lowering the capillary voltage from 3.25 kV to 0.58 kV improves sensitivity by two fold. (fda.gov)

OBJECTIVE To establish criteria for the diagnosis of medium chain acyl-CoA dehydrogenase (MCAD) deficiency in the UK population using a method in which carnitine species eluted from blood spots are butylated and analysed by electrospray ionisation tandem mass spectrometry (ESI-MS/MS). (bmj.com)
Mass spectrometry offers a means to overcome problems with method-dependent bias between competitive immunoassays for aldosterone. (bmj.com)

Anatomy9

Organisms14

Diseases4

Chemicals and Drugs98

Analytical, Diagnostic and Therapeutic Techniques and Equipment72

Phenomena and Processes44

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture4

Information Science8

Named Groups1

Health Care13

Geographicals1

Targeted comparative proteomics by liquid chromatography-tandem Fourier ion cyclotron resonance mass spectrometry. (1/5193)

The identification of 3,4-MDMA from its mass equivalent isomers and isobaric substances using fast LC-ESI-MS-MS. (2/5193)

Parallel ion parking: improving conversion of parents to first-generation products in electron transfer dissociation. (3/5193)

Some structural properties of plant serine:glyoxylate aminotransferase? (4/5193)

Two-dimensional gas-phase separations coupled to mass spectrometry for analysis of complex mixtures. (5/5193)

Newborn screening of inherited metabolic diseases by tandem mass spectrometry. (6/5193)

Peptide-phospholipid cross-linking reactions: identification of leucine enkephalin-alka(e)nal-glycerophosphatidylcholine adducts by tandem mass spectrometry. (7/5193)

Cytoskeletal components of an invasion machine--the apical complex of Toxoplasma gondii. (8/5193)

Tandem Mass Spectrometry

Mass Spectrometry

Spectrometry, Mass, Electrospray Ionization

Chromatography, Liquid

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Chromatography, High Pressure Liquid

Proteomics

Gas Chromatography-Mass Spectrometry

Proteome

Molecular Sequence Data

Amino Acid Sequence

Peptides

Solid Phase Extraction

Electrophoresis, Gel, Two-Dimensional

Spectrometry, Mass, Fast Atom Bombardment

Neonatal Screening

Spectrometry, Mass, Secondary Ion

Reproducibility of Results

Molecular Structure

Reference Standards

Tandem Repeat Sequences

Calibration

Peptide Mapping

Metabolism, Inborn Errors

Limit of Detection

Isotope Labeling

Sensitivity and Specificity

Ions

Sequence Analysis, Protein

Proteins

Databases, Protein

Indicators and Reagents

Complex Mixtures

Indicator Dilution Techniques

Peptide Fragments

Deuterium

Deuterium Exchange Measurement

Chemical Fractionation

Substance Abuse Detection

Trypsin

Chromatography, Reverse-Phase

Isomerism

Protein Processing, Post-Translational

Carbohydrate Sequence

Dried Blood Spot Testing

Isotopes

Biotransformation

Algorithms

Fourier Analysis

Atmospheric Pressure

Metabolomics

Acetonitriles

Oxygen Isotopes

Magnetic Resonance Spectroscopy

Glucuronides

Software

Blood Specimen Collection

Microchemistry

Aconitum

Protein Binding

Phosphopeptides

Electrophoresis, Polyacrylamide Gel

Gases

Analytic Sample Preparation Methods

Carnitine

Oligosaccharides

Cattle

Oxidation-Reduction

Drug Stability

Glycosylation

Glycerophospholipids

Electrophoresis, Capillary

Paper

Polysaccharides

Carbohydrate Conformation

Biological Markers

Models, Molecular

DNA Adducts

Models, Chemical

Drug Residues